LOCUS       DATCGS010000001         3262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_692843, whole genome shotgun sequence.
ACCESSION   DATCGS010000001 DATCGS010000000
VERSION     DATCGS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692843"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VIL39_00005"
     CDS             complement(<1..313)
                     /locus_tag="VIL39_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY5479837.1"
                     /translation="MASADDTPKLRMAPLETAEETAHRCEREQRVVELQRESALEHSL
                     APFRVGSVKYLNGIPLTRGLEEEVTYATPSKLAEMLQRDALDAALVSVVEVLFNDRYD
                     IL"
     gene            complement(313..1482)
                     /gene="mqnE"
                     /locus_tag="VIL39_00010"
     CDS             complement(313..1482)
                     /gene="mqnE"
                     /locus_tag="VIL39_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY5479838.1"
                     /translation="MQFFVQHSKLRDLYDKVAAGQRISEADALRLFESTDLNAVGAIA
                     DLARQRKVGRRASYIVNRYINYSNYCILSCQFCSFARKKRDADGFQLTVEEIVQKARE
                     ALCLGITELHIVGGLHPSLPFSYYTDMLKALRALDSRLQLKCFTAIEVLHLAWLAKKS
                     VAETLGALKEAGLDSLTGGGAEIFRKEVRSAIAQGKESAEEYLDVHRTWHRLGGRSTC
                     TMLYGHVESLADRVDHLRLLRNLQDETHGFVGLVPLPYHPENNDIPVQKPPTGFDTLR
                     TIAVSRIYLDNFDHITAYWVGMGLKLAQVALSYGADDLHGTIIEEHIFHMAGAESPQL
                     QTEAAMIKAIRESGCTPVRRNTFYEPIKVWDAPVAAAVEPSDQRSKVLENNLANA"
     gene            complement(1589..>3262)
                     /gene="uvrA"
                     /locus_tag="VIL39_00015"
     CDS             complement(1589..>3262)
                     /gene="uvrA"
                     /locus_tag="VIL39_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5479839.1"
                     /translation="ETDSDYVRQKIEEYVSQVPCPECKGARLKPTSLAITVGAKNIHE
                     FTLLSVREALGFLGQIQMTDTERLIGGRVIKEIAERLRFLNDVGVGYLTLQRAAATLS
                     GGEAQRIRLATQIGSALVGVLYILDEPSIGLHQRDNDRLLRTLAGLRDLGNSVLVVEH
                     DADTIRHADYILDLGPGAGVRGGELVAAGTLQEVLVHPHSLTAKYLRGELSIPIPRHR
                     VKPSPDRGWLEVLGARENNLKNIDARIPLGTLTCVTGVSGSGKSTLVDDILRRALFRK
                     FFGSKERPGAHRALKGFENLDKVIVIDQTPIGRTPRSNAATYTGMFNHIRNLFARLPA
                     AKIRGYGAGRFSFNVKGGRCEKCQGDGLIKIEMHFLPPVYVTCEACNGRRYNRETLEI
                     TYKGQNIADVLDMTVDEAATFFRAVPQIYEPLLTLAEVGLGYIGLGQPATTLSGGEAQ
                     RVKLAAELSRKATGRTLYILDEPTTGLHFHDVAKLLEVLFKLRASGNTLVVIEHNLDV
                     IKTADWIIDLGPEGGDAGGQIVAQGPPEMVAQCPASYTGQYLASTLAQR"
BASE COUNT          572 a          967 c         1093 g          630 t
ORIGIN      
        1 ccaggatatc gtagcggtcg ttgaagagga cctcgaccac actgaccagc gcggcgtcca
       61 gcgcatcgcg ctgcagcatc tccgccagct tggagggtgt ggcataggtc acttcctcct
      121 ccagaccgcg cgtgagcggg atgccgttaa ggtatttgac cgacccgacg cggaaggggg
      181 ccagcgagtg ttcgagggcg ctctcgcgct gcaattcgac cacgcgctgc tcacgctcgc
      241 aacgatgggc cgtttcttcc gccgtttcga gcggcgccat gcggagtttg ggagtatcgt
      301 cagcggaagc cattatgcgt ttgccaggtt gttctccaga acctttgaac gctggtcgct
      361 gggctcgacc gctgctgcca cgggcgcatc ccatactttg atgggctcgt agaaggtgtt
      421 ccgtcgcaca ggggtgcagc ccgattcgcg gatggccttg atcatcgcgg cctcggtttg
      481 gagttgcggc gattctgcgc cggccatgtg gaagatatgc tcctcgatga tggtgccgtg
      541 gagatcgtca gcgccgtagc tcagcgcgac ttgcgccagt ttgagtccca tacccaccca
      601 ataggcagtg atgtgatcga agttgtccag gtaaatccgg ctgacggcaa tcgtgcgcaa
      661 agtgtcaaag ccggtgggcg gtttctgcac cgggatgtcg ttgttctcgg ggtgataggg
      721 cagcgggaca agaccgacga acccgtgcgt ttcatcctgc aggtttcgca gaaggcgcaa
      781 gtggtccacg cggtcggcga gagattcaac atggccataa agcatggtgc aggtgctgcg
      841 gccgcccagg cgatgccagg tgcgatggac gtcgaggtat tcctcagccg attccttgcc
      901 ctgggcaata gcggaccgga cttccttgcg gaagatctct gcgccgccgc cggtaaggga
      961 atcgagcccg gcttctttca aggcacccag ggtctcagcg acggacttct tcgccagcca
     1021 cgccaggtgc aagacctcaa tcgccgtgaa gcacttcagt tgcagccggg aatccagcgc
     1081 gcggagcgcc ttgagcatgt cggtgtagta gctgaacggc agcgagggat gcaggccgcc
     1141 cacgatgtgc agttcggtta tgcccaggca gagcgcctcg cgggcctttt gaacgatttc
     1201 ctcaaccgta agctggaagc catctgcgtc acgcttcttg cgggcgaagg agcagaactg
     1261 gcaactcagg atgcagtagt tcgagtagtt gatgtagcga ttgacgatgt agctggcgcg
     1321 gcggccaacc ttgcgctggc gggcgaggtc agcgatagcg ccaacggcgt tcaggtccgt
     1381 gctctcgaaa aggcggagcg cgtcggcctc ggagatccgt tggccggcgg cgaccttgtc
     1441 ataaaggtca cggagtttgc tgtgctggac gaaaaactgc acaaactacg attagcagaa
     1501 cgggaaggat tagtaaattg agtttcgagc ggtggagggg tagcctccgc cttcgggcat
     1561 accccattcc cgctccaacc ctcaccgatc agcgttgagc cagggtgctg gcgaggtatt
     1621 gaccggtata gctggccggg cactgggcga ccatttcggg cgggccttgc gcgacgattt
     1681 ggccgccggc gtcgccgcct tccggcccga gatcaataat ccagtccgcc gtcttgatca
     1741 cgtccaggtt atgctcgatg accaccaggg tgttgccgga ggcgcggagt ttgaacagga
     1801 cttcgagcag cttggccaca tcgtggaagt gcaggccggt ggtcggctcg tcgagaatgt
     1861 agagcgtgcg cccggtcgcc ttgcggctca gttccgcggc gagtttcacg cgctgcgcct
     1921 cgccgccgct gagcgtcgtg gccggctgcc ccaggccaat gtagccgagg ccgacctccg
     1981 ccagcgtgag cagcggctca taaatctgcg gtacagcgcg gaaaaacgtg gcagcctcat
     2041 ctaccgtcat gtccagcacg tcggcgatgt tctggccttt gtaggtgatc tccagtgtct
     2101 cgcggttgta gcggcggccg ttgcaggctt cgcaagtaac gtacaccggc ggcaggaagt
     2161 gcatctcaat cttgatcagg ccgtcaccct ggcacttctc gcagcgcccg cctttcacgt
     2221 tgaagctgaa gcgccctgcc ccgtagccgc gaatcttggc ggcgggcagg cgcgcaaaga
     2281 ggttccgaat atggttgaac atgccggtat aggtggccgc gttgctgcgg ggcgtgcggc
     2341 caatcggcgt ttggtctatg acaatgacct tgtcaaggtt ctcaaagccc ttgagggcgc
     2401 ggtgggcgcc cggtcgctct ttggagccga agaacttccg gaacagcgcg cggcgcagaa
     2461 tgtcgtccac cagcgtgctc ttgcccgagc cgctgacgcc ggtgacgcag gtgagggtgc
     2521 cgagcgggat gcgcgcatca atgttcttca ggttgttctc gcgcgcgccg agcacctcca
     2581 gccagccgcg atcgggcgaa ggtttgacgc ggtggcgggg gatgggaatt gacagctcgc
     2641 ctcgcaggta cttggccgtg agcgaatggg gatggacgag cacctcctgc aacgtgcccg
     2701 cagcgacgag ttcgccgccg cgcacgccag cgccaggccc caaatccagg atgtaatcag
     2761 catgacgaat ggtgtcggcg tcgtgctcaa ccaccagcac cgagttgccg aggtcgcgca
     2821 gcccggccag ggtgcggagc aggcggtcgt tgtcgcgctg gtgcaagccg atgctgggct
     2881 cgtcgagaat gtagagcacg ccgaccagtg ccgagccgat ctgggtggcc aggcggatgc
     2941 gctgcgcctc gccgccggag agggtggcgg cggcccgctg cagcgtgagg tagccgacgc
     3001 cgacgtcgtt gaggaagcgc aggcgctcgg cgatctcctt gatgacgcgt ccgccgatga
     3061 ggcgctcggt gtcggtcatc tgaatctgac cgagaaagcc gagcgcctcc cgtacgctta
     3121 gcagcgtgaa ttcgtggatg ttcttcgcgc cgaccgtgat cgccagactc gtcggcttca
     3181 gacgggcccc cttgcactcc gggcagggca cctggctgac gtactcctcg atcttctggc
     3241 gcacgtagtc cgagtcggtc tc
//
LOCUS       DATCGS010000002         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_297142, whole genome shotgun sequence.
ACCESSION   DATCGS010000002 DATCGS010000000
VERSION     DATCGS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297142"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VIL39_00020"
     CDS             complement(<1..915)
                     /locus_tag="VIL39_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5479840.1"
                     /translation="MPTTIHPAPTVTSVCRKPVPLHRGLLRLGLLAAGLCAGLLANAA
                     NWPQYRGLGARGLDESRASPTNWNIETGANIRWQTPVPGLAHASPIVWGDRVYIATAV
                     KAGKAELKVGLYGDITPLEEKESHQWRLLALDKATGKIIWNTLGHEGVPRAKRHPKAT
                     PCNSTPATDGHHIVALFGSEGLFCFDTAGKLVWQKDLGPMDSGFYLVPSAQWGFGSSP
                     IIAEGKVIVLCDVLTNSFLAAFELVDGKEVWRTARKDVPTWSTPTVVETPGRKQIAVN
                     GWHYSGGYDFATGQNLWHLEGGGDVPVPT"
     gene            1208..2284
                     /gene="proB"
                     /locus_tag="VIL39_00025"
     CDS             1208..2284
                     /gene="proB"
                     /locus_tag="VIL39_00025"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY5479841.1"
                     /translation="MRSEILKDITRIVVKLGTGVLTDSRKQPDLAQMEQLVAQVAEQR
                     QAGREIVLVSSGAVGAGMGALGYDKRPSDLAELQACAAVGQSRLMAIYEKLFRAHGLS
                     VAQILLTHDDLQHHERHLNARNTLVTLMNHGVVPIINENDVVSVTELKFGDNDKLSAL
                     VASLLPADLLLILTTVDGVLENFGEANPRTIPLVERVDEALEQKAGGTDSATAVGGMA
                     SKIQAAKIAMRSGIPLVIASGRKKQVMARVVAGEEEGTLFVPQATRLRGRKRWIAFFH
                     HPNGALFVDAGARKALREGGKSLLPPGVTRCDGDFAAGDVVRICDLDGTEFARGIAAF
                     GAAEIKARKLQRVEVVHRDNLVIL"
     gene            2354..3025
                     /locus_tag="VIL39_00030"
     CDS             2354..3025
                     /locus_tag="VIL39_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEY5479842.1"
                     /translation="MKKHSAISDLLKVVARLRAPGGCPWDREQDHMSLRWHAVEEVYE
                     VLDAIEAGDDHELEEELGDLLLQVVLHCQLARERGVFDFEKVARHITDKLVRRHPHVF
                     GTLRVKDVDQVWANWEKIKRAEKQGTRHARSSALDGIPKRLPALLQAAKLVKKAIRAK
                     LMAEVREKPAGTKRELAERLFALAQFAQEQGWSAEDLLRSETKRRERVLRAKEKREAK
                     PSPGS"
     gene            3078..>3426
                     /locus_tag="VIL39_00035"
     CDS             3078..>3426
                     /locus_tag="VIL39_00035"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY5479843.1"
                     /translation="MTDPRYKKLAKLLVQYSTALKRGDRVLLDMIDVPDEFSIELMRA
                     ARAAGATPLIEVRHTRIHREILRATNKEHARLIRDLELFRMKKVQAYIAVRGAGNATE
                     NADVASDRMALYSS"
BASE COUNT          660 a         1008 c         1093 g          665 t
ORIGIN      
        1 cgtcggcacc ggaacgtcgc ccccgccttc caggtgccac aagttctggc cggtcgcaaa
       61 gtcataacct ccgctataat gccagccatt gacggcgatt tgtttgcggc ccggcgtttc
      121 caccaccgtg ggagtgctcc acgtcggcac gtccttgcgg gcggttcgcc aaacctcctt
      181 cccgtccacc agctcaaaag ccgctagaaa tgagttggtc agtacgtcac acaacacaat
      241 caccttgccc tccgcgataa tcggtgaact gccaaaaccc cactgggcgc tgggcacgag
      301 gtaaaagcca ctgtccattg gcccgaggtc cttctgccaa accagcttcc ccgccgtgtc
      361 gaagcagaat aacccttcgc tgccgaagag cgcgacgatg tggtggccgt ccgttgccgg
      421 tgtggagttg cacggggttg ccttcgggtg gcgcttcgcc cgcggcactc cctcgtggcc
      481 gagcgtgttc cagatgatct tgcccgtcgc tttgtccagg gcgagcagcc gccactggtg
      541 ggactctttt tcttccagcg gcgtgatgtc cccgtagagc cccactttaa gctccgcctt
      601 ccccgccttc accgcggtgg caatatagac gcggtcaccc cacacaattg ggctcgcatg
      661 agccagcccg ggcacggggg tctgccagcg gatgtttgcg cccgtttcaa tgttccagtt
      721 ggtcggcgat gcccggcttt catcgagacc tcgagcgccc agcccgcggt attgcggcca
      781 attggcggcg ttggccaata accctgcgca caaaccggcc gccaacagac ccaggcgcaa
      841 gagcccgcga tgtagcggaa ccggtttgcg gcatactgac gtgacggtgg gggctgggtg
      901 aattgtagtt ggcatagtgg tcatcgtttg acaagccccg cggccattcg ttccgccgtg
      961 gggtatgcaa atcagtggca gacccagttt cgccgtggat ctcaaagtgt ttcgggccgc
     1021 cctcaagggg cagtaccctg ccggctaaag ccgggactcc atacaccgca ccaaacccac
     1081 gtttgttatc gaaccgtgcg ctcactcgtt cccttaggaa gcttgagcat caatttcaat
     1141 ctgctcggcc ttcgcgattg ggatttggaa atcgacatcg gcagtggggt ttgctaccaa
     1201 ccttcacgtg cgttctgaaa tcctcaaaga catcacgcgc attgtggtga aactgggcac
     1261 cggggtcctg accgacagcc ggaaacagcc ggaccttgcc cagatggagc agttggtggc
     1321 gcaagtcgcg gagcagcggc aggcgggccg ggagattgtg ctggtatcgt cgggggctgt
     1381 gggcgcgggc atgggggcgc tgggctatga caagcgcccg agcgatctgg cggagttgca
     1441 ggcttgcgcg gcggtgggcc aatcacgcct catggcgatc tacgagaagc tcttccgcgc
     1501 gcatggccta tccgtcgccc agatcttgct cacgcatgat gacctccaac atcacgaacg
     1561 ccatctcaat gcccgcaaca cgctggtgac gctcatgaac cacggcgttg tcccgatcat
     1621 taacgagaat gacgtcgtct ccgttacgga actgaagttt ggcgacaacg acaagctctc
     1681 cgcgctggtc gcctcgctgt tgccggcgga tctgctgctt atcctgacca cggtagatgg
     1741 cgtgctggaa aacttcggcg aggcaaatcc caggacgatc cctctcgtcg agcgggtgga
     1801 cgaagcgctg gaacagaagg ctggcgggac ggacagcgcc accgcggtgg gcgggatggc
     1861 gtcgaagatt caggcagcca agatcgccat gcggtccggg atcccgctcg tgatcgcctc
     1921 gggcagaaag aagcaagtaa tggcccgcgt ggtggcgggc gaagaagagg gcactttgtt
     1981 cgtgccgcag gcgacccggc ttcgtggccg caagcgatgg attgccttct ttcaccatcc
     2041 caacggcgcg ttgttcgtgg atgcgggcgc ccggaaggcg ttgcgcgaag gggggaagag
     2101 cctcctgcca cccggcgtga cccggtgcga cggtgatttt gcggccggtg acgtggtgcg
     2161 gatttgcgat ttggacggca ccgaattcgc tcgcggcatc gccgcttttg gcgccgccga
     2221 gatcaaagcc aggaaactcc aacgggtgga ggtggttcac agggataacc tggtgatact
     2281 gtgagggaac aatggagtaa tggagtattg gagcgttggg gtgctcgttc ccaccactcc
     2341 agccctccgc tttatgaaga aacattctgc aattagcgat ttgctcaaag tcgtggctcg
     2401 gctccgggcg cccgggggtt gtccttggga ccgcgagcag gaccacatgt cgctgcgttg
     2461 gcatgccgtc gaagaggtct atgaggtgct cgatgccatt gaagccggcg acgaccatga
     2521 gctggaagaa gaactgggcg atttgctctt gcaagtcgtt ctccattgcc aactggcgag
     2581 ggaacgcggg gtctttgatt tcgagaaagt cgcccgccac atcactgata agttggttcg
     2641 gcggcatccg cacgtgtttg gcacgctccg ggtcaaggat gtggaccagg tctgggcgaa
     2701 ctgggagaag attaagcggg ccgaaaagca ggggacccgg catgcgcggt cctcggcttt
     2761 ggacggaatc ccgaagcgtt tgccagcgct gctgcaggcc gcgaagctgg tgaagaaggc
     2821 gatcagggcg aagttgatgg cggaagttcg tgagaagccg gctggcacca aaagggagct
     2881 ggctgaaagg ctgtttgcct tggcgcaatt tgcccaggaa cagggatggt cggccgagga
     2941 tttgttgcgg agcgaaacca agaggcgcga gcgtgtgctc cgcgccaagg aaaagcgcga
     3001 ggccaaaccg tcccctggca gttgagaatc tctgctcgac tcctgattcc gcccgtgctt
     3061 acttttcgga acgctttatg actgacccac gttacaagaa gctggcgaag ctgctggtac
     3121 agtattcaac tgccttgaag aggggggacc gtgtcctgct ggacatgatt gatgtgcctg
     3181 acgagttttc gattgaactg atgcgcgcgg cccgggcggc cggcgcgacg ccgctcattg
     3241 aagtccgcca cacgcgcatc caccgcgaaa tactgcgcgc gacgaacaag gagcacgccc
     3301 gcctgatccg cgacctcgag ctgttccgga tgaagaaggt gcaggcctac atcgccgttc
     3361 gcggcgccgg caacgccacc gagaacgccg acgtggccag cgatcgaatg gcgctctatt
     3421 ccagcg
//
LOCUS       DATCGS010000003         5023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_198440, whole genome shotgun sequence.
ACCESSION   DATCGS010000003 DATCGS010000000
VERSION     DATCGS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198440"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1174)
                     /locus_tag="VIL39_00040"
     CDS             complement(86..1174)
                     /locus_tag="VIL39_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5479844.1"
                     /translation="MLQVQLIQPSGFGPSDFLRLSAFGFRLLPLLAALLLLAGCRSVP
                     PEKPLERFAFSHPAMGTLISITLYASDPAAAKAAAAAAFQRIDALEDILSDYQADSEL
                     MRLCDQPFGTPVPVSVELFEVLQRAQKISQLSDGAFDVTVGPYIRLWRFARKRKVLPT
                     PAEIAAARVAVGWQKLRLDQRARTVTLLVPNMRLDLGSIGKGYAADQALRLLKGRGID
                     RALVAASGDIAIGNPPPGQRGWKLGIAAFDGSTNAPPRTLLLHNAGISTSGDTEQFIE
                     INGVHYSHIVNPATGLGMTERIQSTVIGPDATTTDSLDTTVSLLGVGRGLALIDSWPH
                     TAALVVTKEGGRTQTFPSRRFGQIPQVH"
     gene            complement(1242..2579)
                     /locus_tag="VIL39_00045"
     CDS             complement(1242..2579)
                     /locus_tag="VIL39_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5479845.1"
                     /translation="MQDYLTKPLSAFSRRQFIKSSTVIAASAAAAASFPSVLRGQSKQ
                     PINAVVIGVGGRGSGAGRDFTDAAKVIGAEAKIVAVADIFPEAAKKAGQTFEVPEDKC
                     FSGFDAFQKALAVPGVNYAILATPPGFRASHFKAAVEAGKNVFMEKPVAVDGPGCQVI
                     YAAAEMAKQKNLKVAAGTQRRHQAGYLDTIKRIKDGAIGDIVTLRAYWVNGGPIWHRG
                     DQGSTELERQIRNWYHYIWLSGDHVCEQHVHNLDVCNWIMGGHPTKCWGMGARQQLDG
                     KSGEIWDNFAMEYEYPNGVRMYSYCGQINRKWSSVSEGVAGSKGSADPSGRIQPKEGD
                     QWRYKKSEKDISPYVQEHVDLINAIQKDTELNEAKQVTDSTLTAIMGREAAYSGAGVE
                     WDQILNSKFTYGPDILYKDCARMEWGAFRTLQPPMPNMHDILSNPPVVPVVKG"
     gene            complement(2662..4833)
                     /locus_tag="VIL39_00050"
     CDS             complement(2662..4833)
                     /locus_tag="VIL39_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY5479846.1"
                     /translation="MKHSSFLAMAATAVLTVSAMPVLAEQRVPAFIYETPREFFSSGD
                     FDKDGRTDLVVVDKESGKYRLGYQLTPGVFSWVDCRMSGIKGVAGFSLGHLLAANQEA
                     LAFTSPDDNQITLVDVSSSTTPGRPVPVPFTAALGPNTVVAVDIGGDGNTGLDDLYVG
                     SIYNSPDPNQATLLRNDGKEFPKLAEAPLAGAALRGNRLSLKAGQPELLCLLVHGDKA
                     DTLRIEDLSSGKPVAVATAADLPAGSDYAVGSFRGSPLREFLVYKPGDKNLTVRPVEE
                     AAAGKFQFGASQSFDLGQPVRRVVVLDELSAKKLFIIFGEGEKAGVYDFDGAKAPTLV
                     QSLAATNDLFASAASFPGGFIAFSQPAGGKFTTRYQVFKAEGAAYVPGQFGSLASLAD
                     NDNITIPDIHARITAKPAAANDSEMKAYTNTIPGTRVTFAMVPIPGGEFLMGSPDSEP
                     ARKPDEGPQHKVKVSAFWMGQCEVTWNEYELFMYPDEEKRTRVTIPTDAAGDKLADAV
                     THPSKPYVEMSFGMGKDGFPAISMTQHAANKYCQWLSAKTGQFYRLPTEAEWEYAARA
                     GTTTAYFFGDDKAKLGEYAWFEQNSDFKYQKVGKKKPNPWGLYDIYGNVVEWVLDQYN
                     PDFYKQSAANGGVVDPWCRATKPYPHAVRGGSWDDEATMARSAARRGSDRSWKMQDPQ
                     LPKSIWYFSDAQFVGFRVVRPLKVPPPEQMQKYWTSGVERD"
BASE COUNT          957 a         1556 c         1498 g         1012 t
ORIGIN      
        1 cggcggggaa gcgttcaaag aaagcgctaa cccctgagtc tacgggttag tgagtatcat
       61 gaattggccc tgaagtcaac ccagtttagt gcacctgtgg aatctgcccg aatcgacggg
      121 aggggaaggt ctgagtccgc ccgccttctt tggtcaccac cagcgccgcc gtgtgcggcc
      181 aggaatcgat taacgccaga ccacgcccca cgccgaggag gcttacggtg gtgtccaggc
      241 tgtcggtggt ggtggcgtcc ggcccaatga cggtggattg aattcgctcc gtcatgccga
      301 ggccggtcgc ggggttcaca atgtgcgaat aatggacgcc gttgatctca atgaattgct
      361 cggtgtcgcc ggaggtcgag atgccggcgt tgtgcagcag gagggtccga gggggggcgt
      421 tcgtactgcc gtcaaaggcc gcgatgccta atttccaccc gcgctgtcca ggaggagggt
      481 tcccaatcgc gatgtcaccg ctcgccgcca cgagcgcacg gtcgattccc ctgcccttca
      541 gcagccgcaa cgcctggtcg gcggcatagc ctttgccgat gctgcccaag tccaaacgca
      601 tgttcggcac cagcaaggtc accgtgcgcg cgcgctgatc cagccgcagt ttctgccagc
      661 ccaccgccac gcgggcggca gcaatctcag cgggcgtggg gaggactttg cgtttccgcg
      721 cgaagcgcca tagccggatg taaggcccaa ccgtcacgtc gaacgcaccg tcggaaagct
      781 gcgagatctt ctgggcgcgc tggaggactt cgaagagttc cacgctgacg ggcaccggcg
      841 tcccaaaggg ctgatcgcag aggcgcatca gttcgctgtc cgcctggtag tcgctcagga
      901 tgtcttccag cgcgtcgatg cgctgaaagg ccgcggcggc ggcggctttg gccgcagcag
      961 ggtccgacgc gtaaagggtg atgctgatca gggttcccat cgctggatgg gagaaagcaa
     1021 agcgctcaag cggcttctcg ggaggcacag aacggcagcc ggcgagaagc agcagggcgg
     1081 cgagaagggg gagaagccga aagccgaaag ccgaaagccg aaggaagtcc gagggcccga
     1141 aacccgaagg ctgtatgagt tgaacttgga gcattctctg cctaagaaaa aaggccggcc
     1201 aatcaggccg gccttgattt taaaccatgc cgcgcaaccc gtcacccttt aacgaccgga
     1261 accacgggtg gattgctgag aatgtcgtgc atgttaggca tgggcggttg cagagttctg
     1321 aaggcgcccc actccatcct ggcgcagtcc ttgtaaagga tgtccgggcc gtaggtaaac
     1381 ttggagttca gaatctggtc ccattccacg ccggctccgc tataggccgc ctcgcgaccc
     1441 atgatggcgg tgagcgtgct atcagtgacc tgcttggcct cgttcaattc cgtatccttc
     1501 tggatggcat tgatgaggtc aacatgctct tgcacgtaag ggctaatgtc cttctctgac
     1561 ttcttgtagc gccactggtc gccttccttg ggctgaatgc ggccgctcgg atcggcggag
     1621 cccttcgagc ccgcgacccc ttcactgacc gaagaccact tgcggttgat ttgaccgcaa
     1681 taactgtaca tccgcacgcc gtttgggtat tcgtattcca tggcgaaatt gtcccagatc
     1741 tcgcctgact tgccgtccaa ctgctggcgg gcgcccatgc cccaacactt cgtggggtga
     1801 ccgcccatga tccagttgca gacgtcaagg ttgtggacgt gctgttcgca aacatggtcg
     1861 ccgctgagcc agatgtaatg gtaccagttg cgaatttgcc gttcgagctc ggtgctgccc
     1921 tgatcaccgc gatgccagat ggggccgccg ttgacccaat aggcgcgcag ggtgacgatg
     1981 tcgccaatcg cgccgtcctt gatgcgcttg atggtatcga ggtagccggc ttgatggcgg
     2041 cgctgggtgc cggcggcgac cttgaggttt ttctgcttcg ccatttctgc cgccgcgtat
     2101 atcacctggc agccgggacc gtccaccgcc accggctttt ccatgaacac attcttgccg
     2161 gcttcgaccg cggccttgaa atgagaggcg cggaacccgg gcggggtggc cagaatggcg
     2221 tagttcacac caggaaccgc cagcgccttc tgaaaggcgt cgaaaccact gaagcacttg
     2281 tcctcgggca cttcgaaggt ctggcccgcc ttcttagccg cttccgggaa gatatccgcc
     2341 accgcgacga ttttggcttc ggcgccgatc actttggcag cgtcggtaaa gtcccggccc
     2401 gcgccgctgc cgcgcccgcc cacgccgata accaccgcgt tgatgggttg tttgctctgg
     2461 ccgcggagaa ccgaggggaa gcttgcggcg gcagccgccg aggctgcgat gacggttgaa
     2521 ctcttgatga attgacggcg ggagaacgcc gacaacggtt tagttaagta atcttgcatt
     2581 agacctttca agttgttgtt tctatttcag attgcggtcg agaactggtg tatctgctga
     2641 cgacgcgcgg ataccggaga ttcagtccct ttccacgccg cttgtccaat acttttgcat
     2701 ctgttcgggc ggcggcacct taagcggacg cacgacacgg aagccgacga actgtgcgtc
     2761 tgagaaatac cagatgctct tcggcaactg cgggtcctgc atcttccagg agcggtccga
     2821 gcctcgccga gccgcgctgc gggccatcgt agcctcgtca tcccacgaac cgccgcgcac
     2881 cgcatgcgga tagggtttgg tcgcccggca ccacggatca acgacgccgc cgttcgcggc
     2941 ggactgcttg tagaagtcag ggttatactg gtcaagcacc cattcgacga cgttgccgta
     3001 gatgtcgtaa agcccccaag gattgggttt cttcttgccg accttctggt atttgaagtc
     3061 gctattctgt tcgaaccagg cgtattcgcc cagcttggcc ttgtcgtcgc cgaagaaata
     3121 tgccgtcgtc gtgccggccc gcgcggcgta ttcccattcc gcctcagtgg gcaagcggta
     3181 gaactgtccg gtcttggcgc tgagccactg gcaatacttg ttcgccgcgt gctgcgtcat
     3241 gctgatggcc gggaaaccgt ccttgcccat gccgaagctc atctcgacgt agggcttgga
     3301 tggatgcgtc acggcatcag ccagcttgtc tccggcggca tccgtcggga tggtgacccg
     3361 cgtccgcttt tcctcatcag gatacatgaa cagctcgtat tcgttccaag tcacttcaca
     3421 ctggcccatc cagaaggcgg aaaccttcac cttgtgctgc gggccttcgt ccggcttacg
     3481 ggccggctca ctatccgggc tgcccatcag aaactcgccg ccgggaattg gcaccatggc
     3541 aaaggtcaca cgcgtgcctg ggatcgtgtt ggtgtaagcc ttcatctcgc tgtcattggc
     3601 cgcagccggt ttggccgtga tccgcgcgtg gatgtcagga atagtgatgt tgtcgttgtc
     3661 cgccagcgat gccaggctgc cgaactggcc gggaacataa gccgcgcctt ccgccttgaa
     3721 gacctggtag cgcgtggtaa acttgccgcc cgccggctgt gaaaaggcga tgaagccccc
     3781 cggaaaactt gctgcggagg caaacaaatc gttggtcgcc gccaggcttt gcacaagcgt
     3841 gggcgctttc gccccgtcga aatcatacac cccggccttc tcgccctcac cgaatatgat
     3901 gaacagcttc ttcgcgctga gctcgtctaa cacaacgact cgccgcaccg gctggcccag
     3961 gtcgaaggac tggctagcgc caaattgaaa ctttccggcc gcagcctctt ccaccggccg
     4021 cacggtcagg ttcttgtcac ccggcttgta aaccaggaac tcgcgcaacg gcgacccgcg
     4081 aaaacttccc acagcgtagt ccgagccagc gggcaaatcc gccgcagtag ccaccgccac
     4141 tggcttgccg ctgctgagat cctcgatgcg cagcgtgtcc gccttgtctc cgtgcaccag
     4201 caggcagagg agttccggtt ggcccgcttt gagcgagagc cggttacccc gaagagcggc
     4261 accagccaaa ggcgcctcgg ccagtttcgg aaactctttc ccgtcattgc gcaacagggt
     4321 cgcctggttc gggtcgggcg aattgtatat ggagccgaca taaagatcgt ccaggcccgt
     4381 gttcccgtcg ccgccgatgt caactgccac gaccgtattc ggccccagcg ctgccgtgaa
     4441 cggcaccggc accggccggc ccggggtggt cgagctggag acgtccacca gggtgatctg
     4501 attgtcgtca ggggacgtaa aggccagcgc ctcctggttc gccgccagca agtgaccgag
     4561 gctgaacccg gcaacccctt tgatcccgct catccggcaa tccacccagg agaacacccc
     4621 aggcgtcaac tggtagccga gccggtactt gccgctctcc ttgtcaacga ccaccaagtc
     4681 tgtccgccca tccttgtcaa agtcgccact gctgaagaac tcgcgcgggg tttcataaat
     4741 gaaagcaggc acacgctgct cagccaaaac cggcattgcc gaaaccgtca ggaccgccgt
     4801 cgctgccatc gccaaaaaag aagaatgttt catgcttata aaaatgcgga ccacagtgtt
     4861 gtgagcccac gtcagattgt caaacctaga tcgtcccagg ttgtcccccc atcggagtat
     4921 agccttcaca cgtcgtagcc cccccccccg ccaatcccta ttttgtggct tgatttcagc
     4981 tctaaacgcc gtcaaggtgc gcttaaacga atcaagtctg agc
//
LOCUS       DATCGS010000004         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_198472, whole genome shotgun sequence.
ACCESSION   DATCGS010000004 DATCGS010000000
VERSION     DATCGS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198472"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VIL39_00055"
     CDS             complement(<1..1029)
                     /locus_tag="VIL39_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY5479847.1"
                     /translation="MAVAAPSAPRVSVDGKFFRLGEEKFYAKGVAYGPFAPNAAGQPF
                     ASPEQTVRDFAQIRELGANVIRVYHVPARWFLDLAAEYKLKVLIDIPWNKHLCFLDSP
                     AQRAQACEAVRRAVFACARHPSVFAFSIANEIPPDIVRWSGAQAVADFIDALVHEAKR
                     ADPECLCTFTNYPPTEFLRPQSVDFLCFNVYLHQRQPFENYLARLQMLAESKPLLLGE
                     FGIDSLREGEARQGEILAWQIESAFRGGVAGAVLFTFTDDWWRGGRQVEDWKMGLTTR
                     DRQPKPAFWTVQKMFRAAPYFPLPRCPKVSVVVASYNGERTLKACLDSLEQLNYPDYE
                     VILVDDGST"
     gene            complement(1135..>2267)
                     /locus_tag="VIL39_00060"
     CDS             complement(1135..>2267)
                     /locus_tag="VIL39_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5479848.1"
                     /translation="SDSYGSAGPDQIPFDQSRGDAGITLSQPLLKNFWIDQTRLNIRV
                     AKNRVKYSELTLKLQIMQTVTTVEIAYYDLIYDREYVTVQQKAVELATQLVVENRKRV
                     EVGALAPLDQQQAEAQAASTDAALIAAKSALAIQEHLVKQLITDQYSQWANVSVEPSG
                     TLAAPRQSFSLQDSWSKGLSQRPDLLQAKLDIEKQGVTIKYNHNQLFPELDLVGTYGH
                     TAAGFSEYNQYLGQIRDGTFPYYSYGGKLTFPIGNTGARAAYKSSKLVMEQLLLTLKK
                     LEQTIMVQIDNDIKQAESSFQQVAATRTAREYAAAALDAEQKKLESGKSTTYTVLQIQ
                     RDLTTARGNEIQALDSYNKALSQLSFDEGSTLERLGINLEVK"
BASE COUNT          409 a          634 c          748 g          476 t
ORIGIN      
        1 ggtcgagcca tcgtccacca ggatcacttc atagtccggg taattcaact gttccagcga
       61 atccaggcag gccttgagcg tgcgctcgcc gttgtagctg gccaccacga ccgagacttt
      121 tgggcagcgg ggcagcggga agtagggtgc cgcgcgaaac atcttctgca cggtccaaaa
      181 cgccggcttt ggctgccggt cgcgcgtagt cagccccatc ttccagtctt cgacctgccg
      241 cccgccgcgc caccagtcat cagtgaaggt gaagaggact gcgccggcca ccccaccgcg
      301 aaatgcgctt tcgatctgcc acgccaggat ttcaccttgg cgggcttcgc cctcgcgcag
      361 cgagtcgatg ccgaattcgc ccagcagcag gggcttggat tcggccagca tctgcaggcg
      421 cgcgaggtaa ttctcaaacg gctggcgctg gtgcagatag acgttgaaac aaaggaagtc
      481 cacgctttgc gggcgcagaa actcggtcgg cggataattg gtaaaggtgc acaggcattc
      541 ggggtccgcg cgcttggctt cgtggaccag ggcatcgatg aagtcggcga cagcctgcgc
      601 gccactccag cgcacaatgt caggtggaat ctcgttggca atgctgaacg cgaagaccga
      661 cggatggcgg gcgcaggcga agacggcccg gcgcaccgct tcgcacgcct gcgcccgctg
      721 agcgggcgag tcaaggaaac agagatgctt gttccacgga atgtcgatga ggactttgag
      781 cttgtactcg gcggccagat cgagaaacca tctggcgggg acgtgataaa cgcggatgac
      841 gttggccccg agttcacgga tttgggcaaa gtcccgcacg gtctgttccg gcgaggcgaa
      901 cggttggccg gccgcatttg gcgcaaacgg accgtacgcc acgcccttgg cgtagaactt
      961 ctcttcgccc aggcggaaga atttgccgtc cacgctcacg cgtggcgcag agggagccgc
     1021 gactgccatg cgctttagac gagacctggg cctgatttca tcatcgccgc gttggatagc
     1081 agacccccgg gcgcggggca agcgaggagg cgaggttgtg gcagcgattc tcagttactt
     1141 cacttcgaga tttatgccga ggcgttcgag cgtcgaaccc tcatcgaagc tgagctggga
     1201 gagagctttg ttgtagctgt ccagggcctg gatttcgtta ccgcgggccg tcgtgagatc
     1261 tcgctgtatt tgcaggacgg tgtaggtggt gctcttgccg ctctcgagct tcttttgctc
     1321 ggcatccaac gcggcggcgg catactcgcg ggcagtgcgg gtggcggcca cttgctggaa
     1381 actggattcg gcctgtttga tgtcgttgtc aatctggacc atgatcgtct gctcgagctt
     1441 cttgagcgtc agcagcaatt gctccatcac cagcttgctg ctcttgtagg cggcgcgagc
     1501 gcccgtgttg ccgatgggga aggtcagttt cccgccgtag gaatagtacg ggaaggtgcc
     1561 atcccggatt tggcccaagt attggttgta ctcgctgaag cctgcggcag tgtggccgta
     1621 agtgccgacg aggtccaatt cggggaagag ctggttgtgg ttgtatttga tggtgacgcc
     1681 ctgcttttcg atgtccagct tggcctggag caggtctggc cgttgggaca agcctttgct
     1741 ccagctatcc tggaggctga aggactggcg gggagcggcg agtgtgccgg agggctcgac
     1801 gctgacgttg gcccattgag aatactggtc ggtgatgagt tgtttcacca ggtgctcctg
     1861 gatggccaag gcgctcttgg cggcgatcag ggcggcatcg gtggaagcgg cttgcgcctc
     1921 cgcctgctgc tggtccaaag gagccagcgc gccgacttcg acacgcttcc tgttttcgac
     1981 aaccaactgc gtggccaact cgacggcctt ctgctgcacg gtgacgtatt cccggtcgta
     2041 gatgaggtcg taatacgcaa tctccaccgt ggtaaccgtc tgcatgatct gcagcttgag
     2101 ggtcagttcc gagtacttaa cccggttctt ggcgacgcgg atgttcaagc gggtctgatc
     2161 gatccagaaa ttcttgagca ggggctggct gagggtgatc cctgcatcgc cgcgggactg
     2221 gtcaaaaggg atctggtcag gtccggcgct tccgtaagag tctgaga
//
LOCUS       DATCGS010000005         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_446032, whole genome shotgun sequence.
ACCESSION   DATCGS010000005 DATCGS010000000
VERSION     DATCGS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446032"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VIL39_00065"
     CDS             complement(<1..1757)
                     /locus_tag="VIL39_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479849.1"
                     /translation="MKRFVYLLLMLTASTVFCGDAKVVFHDEFEQGSTASPPPGWAMW
                     GAEQYKTPSNYTRDTTNPHGGQACLRIHHPAKSAGYIVSSPDRAINPKPAMVYAVSFW
                     ARAEKPGKATFRWTAYRSIKPFVDAPSPGTFTFDAGGGWKEFSFTVREGLDLFADQSP
                     FLMLTFIAATESGEERTLWIDDVRVTELPDPHPARLVNSEVIPHDPLQHRLRPGDQLE
                     FTVDATKRLRRANQDVGGVSFHRVCGWTGQPYNGKGDYTLAPELETAIREMRLPMTRF
                     YALGDEPFGAEAAIDKVAELLRRVGVPEDRCVLEFERQGAETKLPPETWARGVAYSLR
                     HGYKFCRWEIANEPYASMWGFGQAFPTAETFIAHFRAVSRAIRAVDPQAQIGVDIHRD
                     DVKWGNYLLKELAGGYEFVAPHYYCGANVRELPFEEIALTENYRMLDLALRTEALIRA
                     YNHDCDVYQYDTEWGMICNAPNGKDADFEGRNANIIGTLHRAVRLIYYAREDILRGAS
                     GWQMLSRLNAPGFGILAQEAPDKRFMLYWLYYYFNRHLGEWALATDGVAPYYQPRQEA
                     DRARFAGPLTPALATLSKD"
     gene            complement(1764..>2754)
                     /locus_tag="VIL39_00070"
     CDS             complement(1764..>2754)
                     /locus_tag="VIL39_00070"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEY5479850.1"
                     /translation="KARYRIEATSSFDIEARRERTKFHAANVKRCLYRPFDIRWLYYD
                     PELTSRPAEKVMRHMLAGQNLGLITLRQTRRSETGTFLAARGLINKDAVSIFDIGTVF
                     PLYLYADGHREKKGGGGTMLMALFEPAAAYGRRRANLNPKFIADVTQRLGLKWVPVDR
                     GDLKKTVGPEDIFHYAYAVFHSPTYRTRYAEFLKVDFPRLPLTTDLALFRALAGKGAE
                     LVALHLMESPRLNDFITEFPVKGDNEVEKAHYTDNDRRVWINSSQYFGGVPKAVWDFH
                     VGGYQVCEKWMKDRKTRKLTYEDLQHYQKIVVALQETIRLMAEIDSVSENYGS"
BASE COUNT          521 a          848 c          836 g          549 t
ORIGIN      
        1 tcgtccttgc tcagcgttgc cagcgccggc gtcaacgggc cggcgaagcg ggcgcggtcg
       61 gcttcctgcc gcggctggta atagggggcg acaccatcgg ttgccaaggc ccattcgccc
      121 aggtggcggt taaagtaata gtaaagccag tagagcatga accgtttgtc gggcgcctcc
      181 tgcgcgagga tgccgaagcc gggcgcattg agtcggctga gcatctgcca cccgctcgct
      241 ccccgcagaa tgtcctcccg ggcatagtaa atcagccgca ccgcgcggtg cagcgtgccg
      301 atgatgtttg cgttgcggcc ttcgaagtcg gcatccttgc cgttgggtgc gttgcagatc
      361 atgccccatt cggtgtcata ctgataaacg tcgcagtcgt ggttgtaagc gcggatcagc
      421 gcctccgtgc gcaaggcgag gtccagcatc cggtagttct cagtgagcgc gatttcttca
      481 aacggcaatt cccgaacatt ggccccgcaa taataatgcg gcgccacgaa ctcataacct
      541 ccggcgagtt ccttcagcag atagttgccc catttcacgt cgtcgcgatg gatgtccacg
      601 ccgatctggg cttgcgggtc aaccgcgcga atggcgcggc tgacggccct gaagtgcgcg
      661 atgaaagtct ccgccgtcgg aaacgcctgg ccaaagcccc acatcgaagc gtaaggctcg
      721 ttggcgattt cccagcggca aaacttgtag ccgtgccgca gcgaataggc gacgccgcgc
      781 gcccaggtct ccggcggcaa cttcgtctcc gcgccctgcc gttcgaactc cagcacgcag
      841 cgatcctcag gcacgccgac gcgccgcagc agctcggcga ccttgtcaat cgccgcctcg
      901 gcgccgaagg gctcgtcgcc caaggcgtag aaccgcgtca tgggcagacg catctcgcgg
      961 atggccgttt ccagttccgg cgcgagtgtg tagtcaccct tcccgttgta tggctggccc
     1021 gtccatccgc aaacgcgatg aaacgacacg ccgcccacgt cctgattggc acggcgcaac
     1081 cgcttcgtcg catccacggt aaactcaagc tgatcgcccg gccgcagccg gtgctggagc
     1141 gggtcgtgtg ggatcacctc gctattgacc aaacgggcgg gatgcggatc gggcagctcg
     1201 gtgacgcgca cgtcgtcaat ccacagcgtt cgttcctccc ccgattcggt cgccgctatg
     1261 aatgtgagca tcaggaacgg gctctgatcg gcgaacaggt cgagcccctc gcgcaccgtg
     1321 aacgagaact ccttccaccc gccgccagca tcaaacgtga atgttcccgg cgatggcgca
     1381 tcaacgaagg gcttaatgct ccggtaagcg gtccaccgaa atgtcgcctt gcctggcttc
     1441 tccgcacgcg cccaaaatga caccgcatac accatcgccg gcttcggatt gatggcgcgg
     1501 tcgggcgacg acacaatata cccggcggac ttagcgggat gatggatgcg aagacacgct
     1561 tgtccgccgt gcggatttgt tgtgtcgcgc gtgtagtttg acggcgtctt atactgctcc
     1621 gcgccccaca ttgcccagcc gggcggtgga cttgccgtcg agccctgctc gaactcgtcg
     1681 tggaagacca ccttcgcatc gccgcaaaac accgtgctgg ccgtcaacat gaggagcaga
     1741 tagacgaatc gtttcataat tggctaactg ccgtagttct cgctgaccga gtcaatttcg
     1801 gccatcagcc ggatggtttc ctggagggca acgacgatct tctggtaatg ctggaggtcc
     1861 tcgtaggtca gtttgcgggt cttgcggtct ttcatccact tctcgcaaac ctggtagccg
     1921 ccgacgtgaa agtcccaaac cgctttgggg acgccaccga aatattggct ggagttgatc
     1981 cacacccgcc ggtcgttgtc ggtgtaatgg gccttctcga cctcgttgtc gcccttgacg
     2041 ggaaattcgg tgatgaagtc gttgagccgg ggcgactcca tcaagtgcag cgcgaccaat
     2101 tctgcgcctt tgccagccag tgcgcggaac aatgcgagat cggttgtgag cggcaggcgc
     2161 gggaaatcaa ccttaaggaa ctcggcgtag cgtgtccggt acgtagggga gtgaaacacc
     2221 gcgtaggcat agtggaaaat atcttccggg ccgacggtct tcttaaggtc gccccgatcc
     2281 accgggaccc atttcagccc gagccgttgc gtcacgtcgg cgatgaactt ggggttgaga
     2341 tttgcccgcc gcctgccgta cgcggcagcg ggttcaaaca gcgccatgag catggtgccg
     2401 ccgccacctt tcttttcccg gtggccatcg gcatagaggt aaagcgggaa gacggttcca
     2461 atatcgaaaa tggagacggc gtccttgtta atcaatcctc tcgcagccag gaaggttccg
     2521 gtttccgatc tccgggtctg gcgcaaagtg atgaggccaa gattctggcc cgccagcatg
     2581 tggcgcatga ccttttccgc cgggcggctg gtcaattccg ggtcgtaata aagccagcga
     2641 atatcgaaag ggcgataaag gcagcgcttg acattggcgg cgtgaaactt cgtccgttcc
     2701 cggcgcgcct cgatgtcgaa gctggaggtg gcctcgatgc gatacctcgc cttg
//
LOCUS       DATCGS010000006         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_50167, whole genome shotgun sequence.
ACCESSION   DATCGS010000006 DATCGS010000000
VERSION     DATCGS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50167"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VIL39_00075"
     CDS             <1..388
                     /locus_tag="VIL39_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5479851.1"
                     /translation="VERHDKRSVHTDSIRIDVETDSGVTSVEGAVVLGKPRLLQVDGI
                     YCEAALTGFLIVMKNQDVPGVIGHVGTVLGKNSINIANFSLGRREGTSEAVALVSTDG
                     LVPESVLAQLKENPAVKFARSVEFRG"
     gene            892..1209
                     /locus_tag="VIL39_00080"
     CDS             892..1209
                     /locus_tag="VIL39_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY5479852.1"
                     /translation="MRQTAQRTRKQEPQTPPAKMYRFDARLNEEQKLLIQRAADLEGR
                     TMTDFVLHSAEAAAERAIEKRAMLILTARETETFVNATLNPPDPASVLRKAAREYREN
                     LAR"
     gene            1236..1730
                     /locus_tag="VIL39_00085"
     CDS             1236..1730
                     /locus_tag="VIL39_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018483766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5479853.1"
                     /translation="MESLGASHDRTAFHSGVLELDNYIHRQAGQDARRKVAAPFVMVD
                     SSGSILGYYTLSAYSVLLGELPEPIARKLPRYPLLPATLLGRLAIGSTCRGRNLGRFL
                     LMDALHRSWRTTSEVASVGVVVEALDETARSFYLHHEFVPFQDHPNKLFLAMVTIEKA
                     FRVK"
     gene            complement(1821..>2918)
                     /locus_tag="VIL39_00090"
     CDS             complement(1821..>2918)
                     /locus_tag="VIL39_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5479854.1"
                     /translation="GLNPGFDTRNVIAAEASLQDARYETSAAVNRLYTQTLERMRRIS
                     GVQSAAVALTLPYERPLNDGFRTVDGVDAKQHGGEFVYATATYFETMRIPMLSGRAFS
                     DSDTPRNARVVVVSASFARKYFHGDALGRHLKSGKDVSEIVGICGDVQQHSGLSGGLG
                     PFSVGPTVYLPASQLNDDFVKLVHTWFSPRWVIRTSGPPANLNGQIRTAVAEVDPNLP
                     IAHFRTMDQLRNVQTGSQRYLAALFSILAGLALLLAAIGLYGLISQSIAQRRHEIGIR
                     LALGATARQTIAGAMRPGILLSIVGIGVGLGMSLVAVRFLRSMLWGVRETDPVTFVAT
                     AAILLVVAALASLAPALRILRMDPAATLRNE"
BASE COUNT          546 a          935 c          913 g          524 t
ORIGIN      
        1 ggtggagcgc cacgacaagc gcagcgtgca taccgattcc atccgcatcg acgtggagac
       61 cgacagcggc gtgacctctg tggagggcgc ggtggtgctc ggcaagccgc ggctcttgca
      121 ggtggacggc atctactgcg aggcggcgct cacaggcttc ctgatcgtga tgaagaatca
      181 ggacgtgccg ggcgtgatcg gccacgtcgg cacggtgctc ggcaagaaca gcatcaacat
      241 cgcgaacttc tccctgggac gccgcgaagg gacctcggag gcggtggcgc tggtgtccac
      301 cgatggcctg gtgccggaga gcgtgctggc gcagttaaaa gagaaccccg ccgtcaagtt
      361 cgcgcgcagc gtggagttcc gggggtagct gaagaaaagt cctcgcaaag gcgcaaagct
      421 cgcaaagaga ccactacttt tcgccgggtt ctttgccgga aactcaccgc cgcagccggc
      481 cttcagccat cggcttggac tcaccccgat caacttgtgc ccggactcgg tcgagaaggg
      541 ccattcccgc cccaccgggc gagaagtcct gttcgagttc gaggtcccgc tggaggcgat
      601 cccgctccga aatccaaacg gcgaggctgg tttgctgttc ggacgggtcc ttgatcgcga
      661 ggcgctcgga acttgagcga acccgagacg tgaagtgtta cgagcactta acttccgggc
      721 cgggccgcgc tactcgttcc gcaacgtctc agccgggaga gcatgggcga agtccccggc
      781 gtcttcacct tcgtgatgcg tgggtgccgc cacgggcttt acctgctgcg ctctggaagg
      841 cgcttgattt cgtacggata tttaccgtac actggtcatg gagatcatcg agtgcgacag
      901 accgcccaac gaacccggaa gcaagagccg caaacgcctc cggccaaaat gtatcgcttt
      961 gatgcccggc tcaatgagga acagaagctc ctcattcaga gggcagcgga tctggaaggg
     1021 cgcaccatga cggacttcgt tctgcacagt gccgaagccg ccgcggagcg ggcgattgaa
     1081 aagcgcgcca tgttgatcct taccgcccgc gaaacggaga cttttgtgaa tgctactctg
     1141 aacccgcccg atccagcctc tgtcctgcgg aaagctgctc gcgagtaccg cgaaaacttg
     1201 gctcggtgac gactccgcct cattttgggt atcgggtgga gtccctcggc gcttctcacg
     1261 accggaccgc tttccactcg ggcgttctgg aactcgacaa ctacatccat cgtcaggccg
     1321 gccaggatgc cagacgaaaa gtcgccgcac cttttgtcat ggtcgacagc agtggctcga
     1381 ttctcggtta ctataccctc tcggcttaca gtgttctgct cggtgaattg ccggaaccca
     1441 ttgccaggaa gcttcctcgc tatccgcttc ttccagccac cctgctcggc cggctggcca
     1501 tcggcagcac gtgccggggt cgaaacctcg gtcggtttct cttgatggat gccctgcaca
     1561 gaagttggag aaccacgtct gaggtagcgt cggttggcgt tgtagttgaa gctctggacg
     1621 agaccgcccg atccttctat cttcaccacg aatttgtacc attccaggat cacccaaaca
     1681 agcttttcct cgccatggtc accatcgaaa aggctttcag ggtgaagtga acagcgcaag
     1741 atgcgctggc tggtcattca tgcatcggga aaccgcttgc caacgcgcgc ggctcggatt
     1801 ggaatcgcgg ccgcatccag ctactcgttc cgcagcgtcg cggccgggtc catgcgcaga
     1861 atgcgcagcg cgggcgcgag gctggccagc gcggccacca ccagcagaat cgcggcggtc
     1921 gccacgaagg tgaccgggtc cgtctcgcgc acgccccaca gcatactgcg caggaagcgc
     1981 accgcgacga gcgacatgcc cagccccacg ccgattccca caatcgagag caggattccg
     2041 ggccgcatcg cgccggcgat ggtctgccgc gccgtggccc ccagcgccag ccggatgccg
     2101 atctcgtgcc tgcgctgcgc gatggattgc gagatcaggc cgtacaggcc gatcgccgcc
     2161 aacagcagcg ccagccccgc cagtatcgag aacagcgccg cgaggtagcg ctggctgccc
     2221 gtctgcacgt tgcgcaattg atccattgtg cggaagtggg cgatgggcaa gttgggatcg
     2281 acctcggcca ccgccgtccg gatctgcccg ttcaggttgg ccggcggacc gctggtgcga
     2341 atcacccacc tgggcgaaaa ccaggtgtgg accagtttca cgaaatcgtc gttgagctgc
     2401 gatgccggca gatagacggt cggcccgacc gagaaggggc ccagaccgcc gctcaatccg
     2461 ctgtgctgct gcacgtcgcc gcaaatgccg acgatttcgc tgacatcctt ccccgacttc
     2521 agatggcggc ccagcgcgtc gccgtggaag tacttcctcg caaaactcgc gctcaccacg
     2581 acgacccgcg cattccgcgg agtatcggaa tcgctgaagg cgcggccgga gagcatgggg
     2641 atgcgcatgg tttcgaagta ggtcgcggtg gcatagacga actcgccccc gtgctgtttg
     2701 gcgtccacgc cgtccaccgt gcggaatccg tcgttcagcg ggcgctcgta gggcagcgtg
     2761 agcgccaccg cggcggactg cacgcccgaa atccgccgca tgcgctccag ggtctgcgtg
     2821 tagagccggt tgaccgccgc cgacgtctca tagcgcgcat cctgtaagga cgcctcggcg
     2881 gcgatcacgt tgcgcgtgtc gaagcccgga ttgaggcc
//
LOCUS       DATCGS010000007         3527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_545394, whole genome shotgun sequence.
ACCESSION   DATCGS010000007 DATCGS010000000
VERSION     DATCGS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545394"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1337
                     /locus_tag="VIL39_00095"
     CDS             174..1337
                     /locus_tag="VIL39_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5479855.1"
                     /translation="MKLELSRRTGTILKLALAAMVVVVIAYRLRFAPVPVLGHEIRPG
                     LIAGEVMGTGTLEARVKTTISPRIQERLAEVLVDQNDRVQAGQLLARLDDGELKLQVE
                     AAEAALAAARATTERVRVDEARAQAVEKQARLNYQRTSDLLTTKVAAQADLDKAVEAL
                     QVAEADLKRARAAIGEAESQVVTAEKNLLYRKELLGYTRILSPYNGLVVRRDRDPGGV
                     VVPGGSLLQIISTNEIWVSAWVDETASPDLKPGQPARVIFRSEPGKSYPGEVARLGRE
                     TDRETREFLVDVRVKELPPNWTIGQRAEVFIETGRKTSALTAPQSFIHWRSGNPGVFV
                     NDHGKAGWRKVTLGLRGRDTTELAQGATAGEQIVKPGEDKKQELTDGQRITVR"
     gene            1334..2545
                     /locus_tag="VIL39_00100"
     CDS             1334..2545
                     /locus_tag="VIL39_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5479856.1"
                     /translation="MNLATKDIRHNLGRFALTTVGIGLLLMIVMGMGGIYRGLIAEAK
                     LLVDQIGADLWVVQGSTRGPFAEISRLPASLEDRARAVPGVASARRFVSHTIQREHSG
                     RPLRIVVQGLAWPDDRGDWIPLLAGRPLRQAHYETIADRTLGLPLGEKIKLGKDVYEV
                     VGLTKGMVGSGGDGLAFFTASDALAVQFDTPGETTRLERAARRARVEAQDLGQVQPLL
                     LERSAGPASGIPAVAPPQVSAVMVTLQPGEDAVKVAATFSTWPDITVYTTQQQTDLLL
                     SGMVDKSRRQIGLFRVLLVIISTIIMALIVYTLTLDKIHDIAMLKLIGARNRVIVGLI
                     LQQALLIGALGYGLAYWLGTFAFPRFPRLVVIETPDLLSLAGIVLGISVVASLLGIWK
                     AMRVEPNKVLS"
     gene            2545..3291
                     /locus_tag="VIL39_00105"
     CDS             2545..3291
                     /locus_tag="VIL39_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5479857.1"
                     /translation="MKPNDSSTPAIEAFNLTKVYGSGNTEVVAMNDVSLRVARGEVVA
                     LLGPSGAGKSTLLTAVGLINLPTAGRIVIGGVSVLEADHALVDLRAFRRKHLGFIFQK
                     ANLIPFLKAVENVQIAMEVNDVAPRAARQRAMELLDYLGVADRAANLPDALSGGQQQR
                     VAVARALANHPSLILADEPTAALDSQRGRQVMELFRKVAHEQGAGVIVVTHDQRSLDV
                     FDRTFEMEDGHLKSRQEPDGSIAKDSPHHN"
     gene            3301..>3527
                     /locus_tag="VIL39_00110"
     CDS             3301..>3527
                     /locus_tag="VIL39_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5479858.1"
                     /translation="MKEDNWKPRKVVIVGAGAVGSTFAYALAQSGLADVIALRDANHE
                     LAMGQVLDLAHGQTFFPSVQIREAQAGDYADA"
BASE COUNT          681 a         1080 c         1115 g          651 t
ORIGIN      
        1 gaagcggacg agcaggaatt gagtgcgaag tttgccgagg tggacaagtc catcggcctt
       61 gaggccatcg cccggctgga gcgattcgcc aaaagtctct cacaaggtcc cgcagccccg
      121 ccggctggaa ccagcgccgc acagccgggc aattgcggct gtggttgcac gtcatgaaac
      181 ttgagctttc ccggcgaacc gggacgatcc tcaaactggc cttggcggcg atggtggtgg
      241 tggtcatcgc ctaccgcctc agattcgcgc cggtgcccgt cctgggccat gagatacgcc
      301 ccgggttgat tgcgggcgaa gtgatgggca ccgggacgct cgaagcgcgt gtcaagacca
      361 ctatcagccc acgcattcag gaacggctgg cggaagtgct ggtggatcaa aatgaccgtg
      421 ttcaggccgg ccaattgctc gcacggcttg acgacggcga actcaagctt caggtggaag
      481 cggcggaagc tgccttggct gcggcgcgtg ccacgacgga gcgggtgcgg gtggacgaag
      541 cccgcgcgca agccgtggaa aagcaggccc ggctcaacta ccaaaggacc tccgacctgt
      601 tgaccacaaa agtcgccgcg caggcggatc tcgacaaagc agtcgaggcg ttgcaggtag
      661 ccgaggcgga cctcaaacgc gcgcgtgccg ccatcggtga agccgaaagc caggtcgtta
      721 cggcggagaa gaatctgctc tatcgcaagg agcttctcgg ttacacgcgg attcttagtc
      781 cgtataacgg gctggttgtg cgccgggacc gtgacccggg cggggtggtg gtgccgggcg
      841 gttccctgct gcaaatcatt tccaccaatg aaatatgggt gtcagcctgg gtggacgaaa
      901 cggcgtcccc agacctgaaa cccggccagc ctgcccgtgt catttttcgc tccgaaccgg
      961 gaaagagtta tccgggcgaa gtcgcccgcc tgggccgcga aaccgaccgc gagacgcgcg
     1021 agtttctcgt tgatgtgcgg gtgaaggagc tcccgccaaa ctggactatt ggccaacgcg
     1081 ctgaagtgtt tatcgaaact ggccgcaaaa cctctgcgct cacggcgccg cagtcgttca
     1141 ttcactggcg cagcggcaac cccggcgtgt tcgtgaacga ccacggtaag gcaggctggc
     1201 gcaaggtcac cctcggtttg cgcgggcgag acaccacaga actcgcgcaa ggagcgactg
     1261 ccggcgagca gatcgtgaaa ccgggcgagg ataagaagca ggaactcacc gacggccaaa
     1321 gaatcaccgt gcgatgaacc tcgccactaa agatattcgc cacaacctgg ggcgcttcgc
     1381 gctgacaacc gttggcatcg gtctgctgct gatgattgtc atgggcatgg gcggcatcta
     1441 ccgcggcctg attgccgagg ccaaattgct ggtggaccag atcggtgccg acctgtgggt
     1501 ggtgcaaggc agcacgcgcg gaccctttgc cgaaatctcg cgcttgccgg ccagtctgga
     1561 ggaccgcgcc cgcgccgtac ccggtgtggc cagcgcccgg cgcttcgtct cgcacaccat
     1621 tcaacgtgaa cacagcggac gccccctgcg cattgtcgtg cagggacttg cctggccgga
     1681 tgatcggggc gattggattc cgctgctggc cggacgcccg ctgcggcagg cgcattacga
     1741 gacgattgcc gaccgcacgc ttggcttgcc gctcggcgag aagatcaagc tgggcaagga
     1801 tgtctatgaa gtcgtcggtc tcaccaaagg catggtcggt tccggcggcg atgggctggc
     1861 cttctttacc gccagtgacg cgctggcagt tcagtttgac acgccaggtg aaacgacgcg
     1921 attggagcgt gcggcgcgcc gggcccgcgt cgaagctcag gaccttggcc aagtccagcc
     1981 gctgctgctc gaacgcagcg ccggaccagc cagcggcatt cccgctgtcg ccccgccaca
     2041 agtcagcgcg gtcatggtca cgcttcagcc cggcgaggac gccgtgaaag tggccgcgac
     2101 cttttccact tggcccgaca tcacggtcta taccacgcag cagcaaaccg acctgctgtt
     2161 gtccggcatg gttgacaagt cccggcggca gattggcctc ttccgcgtcc tgctcgtcat
     2221 tatttccacc atcatcatgg cgcttattgt ctatacgttg acactcgata aaatccatga
     2281 catcgcgatg cttaagctca tcggcgcgcg caaccgcgtc atcgtgggct tgattctcca
     2341 gcaggcactg ctcatcggcg ccctcggcta cgggctggcg tattggctgg gtacgtttgc
     2401 gtttccccgg ttccctcgcc tggtggtgat tgagacccca gacctgctgt ctctcgcggg
     2461 tatcgtcttg ggcatttcgg tggtggccag cctgcttggc atctggaaag cgatgcgggt
     2521 ggagccaaac aaagtcctgt cgtaatgaag ccgaacgatt ccagcacacc tgccattgag
     2581 gctttcaacc tgacgaaagt ttacggcagc ggcaacaccg aggtcgtggc catgaacgac
     2641 gtttcgttgc gcgtggcgcg cggcgaagtg gtggcgctgc ttggccccag cggggccgga
     2701 aaatccactc tactcaccgc cgtcggcctc atcaacctgc ctacggcagg ccgcatcgtg
     2761 atcggcggcg tgtccgtcct ggaagccgac cacgccctgg tggacttgcg cgcgttccga
     2821 cggaagcatc tcggtttcat cttccaaaag gcgaacctga ttcccttcct aaaggccgtc
     2881 gagaacgtcc agatcgcgat ggaagtgaac gacgtcgcgc cgcgcgcagc ccgccagcgc
     2941 gcgatggaac tactcgacta cctcggcgtg gccgatcggg cggccaatct accggacgct
     3001 ctctccggcg gccagcagca gcgcgtagcc gtggcccgtg cgctcgcgaa ccacccaagc
     3061 ctcattctgg ccgatgaacc caccgccgcg ctcgacagcc agcggggccg gcaggtgatg
     3121 gagttgttcc gcaaagtggc gcacgaacag ggggctggcg tcatcgtcgt cacgcatgac
     3181 cagcggtcgc tggacgtttt tgatcgcaca ttcgagatgg aagatggcca tctgaagtct
     3241 agacaagagc ccgatggttc gattgccaaa gacagtccgc accacaactg aggaacatta
     3301 atgaaagagg ataattggaa accaagaaag gtggtaattg ttggcgccgg tgctgtaggt
     3361 tccacttttg cctacgcgct ggcccagagt ggtttggccg atgtgatcgc cttgcgcgat
     3421 gccaatcatg aactggccat gggacaggtc ctcgatttgg ctcatggcca gaccttcttc
     3481 ccttccgtcc agattcgtga agcccaagca ggggattatg ccgatgc
//
LOCUS       DATCGS010000008         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_545427, whole genome shotgun sequence.
ACCESSION   DATCGS010000008 DATCGS010000000
VERSION     DATCGS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545427"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2444)
                     /locus_tag="VIL39_00115"
     CDS             complement(<1..>2444)
                     /locus_tag="VIL39_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY5479859.1"
                     /translation="IFTPQIFSGLRDTVKVLRDLVRENAALATLDAELEQAPSNLRAA
                     FTLLERATGQATKIAASLANEEEELKGWGQTLQRNCEEHLEELRFLAPWLPLPIPTRS
                     SRREEAQTPPSLAAKEDQSLLTSAATRLEEKLAQLDQAPTLREVSTWDQSLSPLIEEG
                     VGLAPWNNERHTPSKLLPFYSTGQAELSPRGITSAKPRTDSPRVITGAEARADSEPPI
                     TRGEERSIPRGERCLREASDRARQRLLALETLAKQSDELAAAMDFTFLFDSTRDLFSI
                     GCNVTERRCDTSFYDLLASEARLCSYVAIALGQVPQDHWFSMGRLLVASRGEPILVSW
                     SGSMFEYLMPLLVMPNYENTLLDHTCQAAVQQQIEYGQLRGVPWGLSESGYNRTDVHL
                     NYQYRAFGVPGLGLKRGLAEDLVIAPYATALALMVAPVEACENLQRLAAEGCAGAYGF
                     YEAVDYTPARLPPGETSVTIRSFMAHHQGMSLLALVNLLQDRPMQRRFMACPLLKAAD
                     LLLQERVPKTAASVFAEDLELETSRMFAGNGEAVMRVFTNPTPPAPEVHLLSNGRYHV
                     VISSAGGGYSRWRDLAVTRWREDATRDCWGTFVYLRDMATGEFWSTAYQPTLRATKGY
                     EAIFTQARAEFRQRHVGLEIHTEISVSPEDDVELRRITVTNRSPVARVIELTSYAEVV
                     LAIPAADAAHPAFSNLFVQTEFVRKSSAILCTRRARSQEEKPPWLLHLMVGQGGEQGE
                     ISCETDRSRFVGRGGTLANPAAMQSMAPLSNTVGSVLDPIISLRRTVTLPPHETAIID
                     LVLGVTESREAALAHV"
BASE COUNT          486 a          741 c          744 g          473 t
ORIGIN      
        1 cacatgcgcc agcgcggctt cccggctttc ggtcacgccc agaaccagat caatgatggc
       61 ggtttcatgg ggcggcaaag tgaccgtgcg ccgcagcgaa atgatgggat cgagcacgga
      121 accgacggtg ttggacagtg gcgccatgct ctgcatcgcg gcagggttgg ccagggtgcc
      181 gccgcgtccg acaaatctgg agcgatccgt ttcgcaggag atttcgccct gttcaccccc
      241 ttgacccacc atcagatgca gcaaccacgg cggcttttct tcctgggaac gggcacggcg
      301 ggtgcagaga atggcggaag attttcgaac gaattcggtt tgcacgaaga gattgctgaa
      361 cgcgggatgc gccgcgtctg cggccggaat cgcgagcacc acttccgcgt aactggtcaa
      421 ttcaatgacg cgcgccacgg gtgaacgatt ggtgactgtg atgcgccgca actccacgtc
      481 atcttccggc gacacgctga tctcggtgtg gatttcgaga ccgacatgac gttgacgaaa
      541 ctcggcgcgc gcctgcgtga agatggcttc gtagcctttc gtcgcgcgta aggtgggttg
      601 atacgcggtg gaccagaatt ctcccgtcgc catgtcgcgc agataaacaa acgttcccca
      661 gcaatcacgc gtggcgtcct cgcgccagcg ggtgaccgcc agatcgcgcc agcgactgta
      721 gccgccgccg gcgctgctga tgacgacatg atagcgacca ttggacaaaa gatgaacctc
      781 cggtgccggt ggtgtgggat tcgtgaaaac gcgcatgacg gcttcgccgt ttccggcgaa
      841 catccgcgat gtctccaatt ccaaatcttc ggcgaacacg ctcgcggcgg tcttgggcac
      901 ccgttcctgc aacagcaggt ccgccgcctt gagcaacgga caggccatga agcgccgttg
      961 catggggcgg tcttgcaaca aattcaccag cgccagcaga ctcatccctt gatggtgcgc
     1021 catgaaggat cggatggtga cgctcgtttc gcccggcggc agacgcgccg gcgtgtaatc
     1081 caccgcttcg tagaaaccgt aagcgcctgc gcacccctcg gccgccagcc gttgcaggtt
     1141 ttcacaagct tcaaccggcg ccaccatcag cgccagcgcg gtggcgtaag gagcgatcac
     1201 caagtcctcg gccaatcctc gtttcaaacc caggcccggc acgccgaagg cgcggtattg
     1261 gtagttcagg tgaacgtcgg tccggttgta gcccgattcg gaaaggcccc acggcacgcc
     1321 gcgcaattgc ccatactcga tttgttgttg gaccgccgcc tggcaggtgt ggtcgagcaa
     1381 agtgttctca tagttgggca tgaccagcag cggcatcaga tattcgaaca tcgagccgct
     1441 ccacgaaacc aggatcggct cgcctcgcga agcgacaagc agacggccca tcgagaacca
     1501 gtggtcctgc ggcacctgcc ctaaagcgat ggcgacgtag ctgcacaacc gcgcctccga
     1561 ggccagcaga tcatagaagc tggtgtcaca ccggcgttcg gtgacgttgc acccgatgga
     1621 aaacaaatcc ctcgttgagt cgaacagaaa agtgaaatcc atcgcggcgg ccagttcgtc
     1681 gctttgcttg gccagggttt ccaacgcgag cagacgctgg cgggcgcggt cgctggcttc
     1741 acgcaggcaa cgctcgcccc gtggaataga acgctcctcg ccccgtgtaa taggcggctc
     1801 cgagtcggcg cgggcttccg cgcccgttat tacacggggc gagtctgtgc ggggtttcgc
     1861 gctcgttatt ccacggggcg agagttcagc ttgccccgtg gaatagaacg gtaggagttt
     1921 ggacggggta tggcgctcat tattccacgg ggcgaggcca acgccttctt caatcaacgg
     1981 actgagcgat tgatcccacg tcgagacttc acgcaaggtg ggtgcctggt cgagttgggc
     2041 gagcttctcc tcaagccttg tagcagccga cgtgaggagg ctctgatctt ctttcgccgc
     2101 aagggaagga ggagtttgag cctcctcacg tcggctgcta cgagttggga ttggcaaagg
     2161 cagccacgga gcgagaaacc gcaattcttc caaatgctcc tcgcaattcc gctgcagagt
     2221 ctggccccac cctttgagtt cttcctcttc gttcgccagc gaagcagcga tcttggtcgc
     2281 ctggcccgtc gctctttcca acaaggtaaa cgccgcgcgc agattggaag gtgcttgctc
     2341 caattctgcg tcgagcgttg ccagcgcagc attttcgcgg accaaatccc ggagcacctt
     2401 caccgtgtcg cgcagaccgg aaaaaatctg tggcgtgaaa attt
//
LOCUS       DATCGS010000009         6491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_100032, whole genome shotgun sequence.
ACCESSION   DATCGS010000009 DATCGS010000000
VERSION     DATCGS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100032"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..930
                     /locus_tag="VIL39_00120"
     CDS             418..930
                     /locus_tag="VIL39_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY5479860.1"
                     /translation="MEWHFESARSAVVARDPVLEAIHRYKYQRAFWFEPFLADLLIQA
                     AGPALAGKKRDMIVPVPLHPTKQREREFNQAERLADRLGAATRIPVNKRLLRRVVLTR
                     TQALLKRPERLANVRNAFAMRDGQRLNGERLVLVDDVFTTGATTGACAKVLVAAGAGE
                     VCVWTVARGI"
     gene            1038..1970
                     /gene="accD"
                     /locus_tag="VIL39_00125"
     CDS             1038..1970
                     /gene="accD"
                     /locus_tag="VIL39_00125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY5479861.1"
                     /translation="MTTTPFTKRTLGRETAEPAVTPPPATNNTTFAKKPKLPGHKSRK
                     REIPEGLWSKCPKCATMVFDKELDENLKVCSHCQYHFPIGARERIHSMVETCTFEEMD
                     AEMTSVDVLNFTGVASYVSKLEDYQKSTSLKDAVITGICKIGEHRAALGVMDFSFLGG
                     SMGSVVGEKLTRLIEKATEKTLPLIIISTSGGARMYEGMFSLMQMAKTCAALAYHAQA
                     RLPYISVLTHPTTAGVMASFASVGDLIIAEPGAMIGFAGPRVIKDTTQAELPPGFQTT
                     EFLLDHGLIDAIVSRKEMKPRLIEYLNYLTGGQN"
     gene            2007..4106
                     /locus_tag="VIL39_00130"
     CDS             2007..4106
                     /locus_tag="VIL39_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5479862.1"
                     /translation="MIRSLFAPALFAPALAWFLFVVCLGQSVLSAEPQADPAEKRPAV
                     KDFNTPREFPTIASREQWQARAKEIREQVLVSCGLWPLPEKTPLQPYIFGKIERDGYS
                     VEKVYFQPLPGFYLAGNLYRPLGRGKGPFPAILNPHGHWPEGRLTDTKDGSAAARCIS
                     FARQGMIAFSYDMVGYNDTFFPDYAGVPADKHSTRHRHFATKRVDLLYNVSLMGLQTW
                     DSIRALDFLASLPDVDRKRLACTGESGGGTQTFMLGAVEDRLAVQAPVVMVSHTMQGG
                     CLCENAPGLRVEYSNMEIAAAAAPRPQLLVAATGDWTKDTLTIEGPAIEHIYHLLDAP
                     DKLRYVRFDFGHNYNQTSREAVYDWFGKWLLKHPNPASLKEAPYQKEPDANLRVFPDG
                     KLPEDARSEARIISLLKEGHREQWESLVVRNKTGLRKFKRLIQPAWRHTLQVEWPNSQ
                     VRSHAEQVKESAGLTAATLRISRGESDTGILATYWAPPGILSNPAPKVVVICSDKSAA
                     APPNLDAKPAGLPLTLLQRGLAVLMVDRFSSGAPPNQFTNFYVTYNRTELQERVRDLL
                     TVCAAAGSMTDPRGPRSFDVTLLGTGRAGLWALLAAPAAGAVIADCDGLDVTSDEALL
                     APDLFCPGIRNIGTFEGGAMLAAPHPLLMHNTGENFPTEALRSTYRAMGASDKLRVVG
                     AALSDEELARWITQARQ"
     gene            complement(4228..5370)
                     /locus_tag="VIL39_00135"
     CDS             complement(4228..5370)
                     /locus_tag="VIL39_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY5479863.1"
                     /translation="MINPRLLKKLAEEHGTPLFVVDHDQLRRNYAQFKRYLPRVQAYY
                     AVKANSDPAIVKTFYDAGCSFDVASMPEFRIVYQYIKDLPDKQRQDWIWDKIIYANPI
                     KANETLQDLNQYKPLVTYDNYEEIKKIKKYAPLAGLALRIKVPNTGAMVELSSKFGAS
                     PGECVDMILAAAKAGLVVEGLSFHVGSQTTNFENYVAAINLAANLFKEARDRGYTKMN
                     LLDIGGGFPAPYDDSVKPFRELAKTINRELDRLFPKNIQILAEPGRFLVATAAVAVSK
                     IIGKAVRDGKLCYYINDGVYHTFSGVIFDHCHYHLKSFKRGPAQICSVFGPTCDALDV
                     VSMAEQLPAHLERGDLLYSENIGAYSHASSTWFNGFAPAKVVHINQ"
     gene            complement(5372..>6491)
                     /locus_tag="VIL39_00140"
     CDS             complement(5372..>6491)
                     /locus_tag="VIL39_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5479864.1"
                     /translation="PYENAKGASPQQMTLYWRQMNIRRMTAKWAQPGPTAVIEHGANP
                     GLISHFTKQGLLDIGNRAIKDGKVKGKAADEIQQLIAEQRFNHLAMKLGVKVIHVSER
                     DTQISNKPKQVGEFVNTWSVEGFREEGTTTAEMGWGTHEKELPPLAYEHTEGPRNQIC
                     LARMGMNTWVTSWVPNYCIHGMVVRHGEAFTISDRLTVWEKGKAIYRPTVHYAYCPSD
                     AAIASLNELRGNDYQLAGKQRILNDEIVSGSDILGALLMGHAYGAWWTGSDLSIEESR
                     RLLPHQNATTMQVAISVVSAAMWMIENPDRGVCAPDDLPHEYVLKVAKPYLGKWISKP
                     SDWTPLLHYTNTFPGHNNPQIDPDDPWQFKNFLITDGD"
BASE COUNT         1206 a         1919 c         2135 g         1231 t
ORIGIN      
        1 atgaacgacc gctacaccca tcacgccttg aagccgctgg aacaggcaat gtcggtgctg
       61 ccatctttca acgaggaggc cgaagagccg acgcagaaga agcagcccaa gtgacgtacg
      121 cggaggcccg caaccagttc catttgtcaa agatcgccct cagccgacac ccatttgggg
      181 attgaaaagc cgtccgatga ggccaagctg agcccgtctc atgatgatga tacggcaatt
      241 gcaggcgctg acggaagagc ttgaagaacg gctgcgcggg cgaccccggc ggagggctac
      301 gtttgcgacg ggtgccgggc caaggtgcga gttatcaagg cgccattctg tgagcggtgc
      361 ggccggccat acgaaggggc tatcaccaac gagtttgaat gcgccaattg ccgggggatg
      421 gaatggcatt tcgagtcggc gcgttcggcg gtagtggcgc gcgacccggt gctggaggca
      481 attcaccgtt acaaatatca gcgggcgttc tggttcgagc cgttcctggc cgatctgctc
      541 attcaagcag cgggaccggc attagccggg aagaagcggg atatgattgt gccggtgcca
      601 ctgcatccca cgaaacagcg cgagcgcgaa ttcaaccagg ccgagcgcct ggccgaccgg
      661 ctgggcgccg caacgcgaat tcccgtgaac aaacggctgc tgcgacgcgt cgtccttacg
      721 cgcacgcagg cgttgttgaa gcgcccggag cggctggcaa atgtccgcaa cgcctttgcc
      781 atgcgggacg gccagcgcct gaacggcgag cgcctggtgc tggtggacga cgttttcacg
      841 acgggcgcga cgaccggcgc gtgcgccaag gtgctggtgg cggctggggc gggcgaagtt
      901 tgcgtctgga cagttgcccg cggaatttga atattgttgc tctgaagctt ggaatgagat
      961 tcgccgtgaa cataaagagc gcgagccggg cggggcagcc cgcgacgcaa gccccttaac
     1021 ctccgacgtt tctaagaatg acaacgaccc ccttcacgaa gaggacgctt gggcgggaga
     1081 cggctgagcc ggcggtcacc ccgccgcccg cgaccaataa cacgacgttt gccaagaagc
     1141 ccaagctccc cggccacaaa tcccggaagc gggagattcc cgagggcctg tggagcaagt
     1201 gcccgaagtg cgcgaccatg gtgttcgaca aggaactgga cgagaatctg aaagtctgct
     1261 cgcattgcca gtatcatttt cccattggcg ctcgcgagcg catccattca atggtggaga
     1321 cgtgcacatt cgaggagatg gacgccgaga tgacgagcgt ggacgtgctc aacttcactg
     1381 gggtggcctc ctacgtctcc aagctggagg actatcaaaa atcaaccagc ctgaaggacg
     1441 cggtgatcac ggggatctgc aagatcggcg agcaccgggc agcgctgggc gtgatggatt
     1501 tcagtttcct gggcggctcg atgggatcgg tcgtgggcga gaagctgacg cggctgatcg
     1561 aaaaggcgac cgagaagacc ctgccgctca tcatcatttc gaccagcggc ggagcgcgca
     1621 tgtatgaagg catgttcagc ctcatgcaaa tggccaagac ctgcgccgcg ctggcttatc
     1681 acgcccaggc gcggctgccg tatatcagcg tgctgacgca cccaacgacg gcgggtgtga
     1741 tggccagctt cgccagcgtg ggggatctca tcattgccga accgggcgcc atgatcggct
     1801 tcgccggccc gcgcgtcatc aaagacacca cccaggcgga actgccgccc ggcttccaga
     1861 ccaccgagtt cctgctcgac cacggtctca ttgatgccat tgtatcgcgc aaagagatga
     1921 agccgcggct catcgagtat ctgaattacc tgacgggcgg ccagaactga gatccgaagc
     1981 ccaaaatcct gaaccagagc gctattatga taaggagcct ttttgcgcct gcactttttg
     2041 cgcctgcact tgcctggttc ctgttcgtcg tttgcctggg tcaatccgtc ttgtcggctg
     2101 agcctcaagc ggaccctgcg gagaaacggc cggcagtcaa ggacttcaac accccccgcg
     2161 agttcccaac gattgcctcg cgggagcaat ggcaggcacg ggccaaagag attcgcgagc
     2221 aggtgctggt gagctgtggc ctgtggccgc tgccggagaa gaccccgctt cagccgtata
     2281 tcttcggcaa gatcgagcgc gatgggtaca gcgtggagaa ggtctatttc cagccgctgc
     2341 ccggctttta cctggcaggc aacttgtatc gccccctcgg gcggggcaag gggccattcc
     2401 ccgccatact caacccgcac ggccattggc cagagggccg gctgacggac accaaggacg
     2461 gcagcgcggc ggcccgctgc atcagcttcg cgcgccaggg catgatcgcg ttttcctacg
     2521 acatggtggg ctataacgac accttcttcc ccgattacgc cggcgtgccg gccgacaagc
     2581 attctacgcg ccaccgccat ttcgcgacga agcgggtgga cctgctctat aatgtgagcc
     2641 tgatggggct gcaaacgtgg gacagcattc gggcgctgga tttcctggcg tcgctgcccg
     2701 acgtggaccg caaacggctg gcctgcacgg gggaatccgg cggcggcacg caaaccttca
     2761 tgctcggcgc ggtggaggac cggctggccg tccaggcgcc ggtggtgatg gtctcgcata
     2821 caatgcaagg cggctgcttg tgcgaaaacg cgccggggtt gcgcgtggag tactcgaaca
     2881 tggaaatcgc cgccgccgcc gcgccacgcc ctcagttgct ggtcgcggcg accggggatt
     2941 ggaccaaaga cacgctgacg atcgagggtc cagccattga gcacatctac cacctccttg
     3001 atgcgccgga caagctccgc tacgtgcgct tcgacttcgg ccacaattac aatcagacca
     3061 gccgggaggc ggtttatgac tggttcggga agtggctatt gaagcatccc aaccctgcct
     3121 cgctcaaaga agcgccgtat cagaaggagc cggacgccaa tttgcgggtg tttcccgacg
     3181 gcaagctgcc cgaagacgcg cgaagtgagg cgcggatcat cagcttgctg aaggagggtc
     3241 accgggagca gtgggaatcc ctcgtggtca ggaacaagac tggtttaagg aagttcaaga
     3301 ggctgataca gccggcctgg cggcacacgc tgcaagtcga gtggccgaac agccaggtgc
     3361 ggagccacgc cgaacaggtg aaagaatccg ccggattaac tgccgccacg ctcaggatta
     3421 gtcgcgggga aagcgacaca ggcatactgg cgacctattg ggcgccaccg ggcatcctca
     3481 gcaacccggc gcccaaggtg gtggtcatct gcagcgacaa gagcgccgcg gcgccaccga
     3541 acctcgatgc caagcccgcg gggctgccgc tgaccctcct ccagcgcggc ctggcggtgc
     3601 tcatggtgga tcgcttcagc agtggcgcac cccccaacca attcaccaat ttctatgtga
     3661 cctacaatcg cacggaactg caggagcgtg tgcgcgacct gctgacggtt tgcgcggctg
     3721 ccggctcgat gacagatccg cgcgggccgc gctccttcga tgtgaccctc ctgggcaccg
     3781 gtcgcgcggg tctctgggcc ttgttggctg cgcctgcggc gggagccgtc attgccgatt
     3841 gcgatggact ggatgttacg agcgacgagg cgctgctggc gccggacctg ttttgcccgg
     3901 gcattcgaaa catcggcacg ttcgaaggcg gcgctatgct cgcggcgcct catcccttgc
     3961 tcatgcacaa taccggcgag aatttcccga cggaagccct gcgctcgacc taccgcgcga
     4021 tgggcgcgag cgacaagttg cgcgtggtgg gcgccgccct gtcagatgag gaactggcga
     4081 ggtggattac tcaagcacgg cagtgaatca gccggaaggg cgtgcgcgag acgtggcggc
     4141 gcttcggctg ccgccgtcgg atttgctccg ccgccagtca ccgattacca atcacccgtc
     4201 accaatgtca gttatcggcg ccgcgcttta ctgatttatg tgcacgacct tcgccggcgc
     4261 aaagccgttg aaccaggtcg aagaggcgtg gctgtaggcg ccgatgtttt cactgtagag
     4321 caggtcgccc cgttcgaggt gggcggggag ttgctcggcc atggaaacca cgtcaagcgc
     4381 gtcgcaggtc gggccgaaca cggagcagat ttgcgccggc ccgcgtttga acgacttgag
     4441 atggtagtgg cagtggtcga agatgacacc ggagaaggtg tgatagacgc cgtcgttgat
     4501 gtagtaacag agtttcccat cgcggacggc cttgccgatg atcttggaga cggccacggc
     4561 ggcggtcgcc acgaggaacc ggcccggctc ggcgaggatt tggatattct tcgggaagag
     4621 gcgatcgagt tcccggttga tggtcttggc cagttcgcgg aagggcttga cgctgtcgtc
     4681 atagggagcg gggaagccgc caccgatgtc gagcaggttc atcttcgtat agccgcggtc
     4741 cctggcttcc ttgaagaggt tggcggccag gttgatggcg gccacgtagt tctcgaagtt
     4801 ggtggtctgg ctgccgacat ggaagctcag gccctcgacg accaggcccg ccttggcggc
     4861 ggcgaggatc atatcaacgc attcgccggg cgaggcgccg aacttggagg agagttcgac
     4921 catcgcgccg gtgttgggca ccttgatgcg cagggccagt cccgccagcg gggcgtactt
     4981 cttgatcttc ttgatctcct cgtagttgtc gtaggtgacc agcggcttgt attggttgag
     5041 gtcctgcagg gtttcgttgg ccttaatggg attggcgtag atgatcttgt cccagatcca
     5101 atcctgccgc tgcttgtcgg gcaggtcttt gatgtattga tagacgatgc ggaactccgg
     5161 catggaggcc acgtcgaagc tgcacccggc gtcgtagaag gtcttcacga tggccgggtc
     5221 ggagttggct ttgacggcgt agtaggcctg cacgcggggc aggtagcgct tgaactgggc
     5281 gtagttgcgg cgcaattggt cgtggtccac cacaaacagc ggcgtgccgt gttcttcggc
     5341 cagtttcttg agcagtcgag gattgatcat gttagtctcc gtcggtgatg aggaagttct
     5401 tgaactgcca ggggtcgtcc ggatcaattt gcgggttgtt gtggcccggg aatgtgttgg
     5461 tgtagtgcag cagcggcgtc cagtcggagg gcttggagat ccatttcccc aggtaaggct
     5521 tggcgacctt aagcacatac tcgtgcggca ggtcgtcggg cgcgcacacg ccccggtccg
     5581 ggttctcaat catccacatc gccgcgctga caacggagat ggccacctgc atggtcgtgg
     5641 cgttctggtg cgggaggagc cggcgcgatt cctcgatgct gaggtcgctg cccgtccacc
     5701 acgcgccgta agcgtgaccc atcagaagcg ccccgaggat gtcggaaccg ctgacgatct
     5761 cgtcgttcag gatgcgctgc ttcccggcga gctggtaatc gttgccgcgc agttcattca
     5821 gcgacgcgat ggccgcgtcg gacgggcaat aggcgtaatg caccgtgggc cggtagatcg
     5881 ccttgccctt ctcccagacc gtcaaccggt cggagatggt gaacgcctcg ccgtggcgca
     5941 cgaccatgcc gtggatgcag tagttgggca cccaggaggt tacccaggtg ttcatgccca
     6001 tgcgggccag gcaaatctgg ttgcgcgggc cttcggtgtg ctcgtaggcc agcggcggca
     6061 gctccttttc gtgcgtgccc cagcccatct cggcggtggt cgtgccttcc tcgcggaagc
     6121 cctcgacact ccaggtgttg acgaactcgc ccacctgttt cggtttgttg ctgatctggg
     6181 tgtcgcgctc gctgacatgg atcaccttca cgccaagctt catggccagg tgattgaacc
     6241 tctgctcggc gatgagctgc tggatctcgt cagcggcctt gcccttgacc ttcccgtcct
     6301 tgatggcccg gttgccgatg tccagcaggc cctgcttggt gaagtgcgag atgaggccgg
     6361 ggttggcgcc atgctcgatc accgccgtgg ggccgggctg agcccacttg gcggtcatgc
     6421 ggcgaatatt catctgccgc cagtagagcg tcatctgctg cggcgacgcg cccttggcgt
     6481 tctcgtaagg g
//
LOCUS       DATCGS010000010         5419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_446412, whole genome shotgun sequence.
ACCESSION   DATCGS010000010 DATCGS010000000
VERSION     DATCGS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446412"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="mutS"
                     /locus_tag="VIL39_00145"
     CDS             <1..1284
                     /gene="mutS"
                     /locus_tag="VIL39_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY5479865.1"
                     /translation="GRAIVDEPPLAVKEGGMIRDGFDPALDELRTAMRGGKDWIAKLQ
                     QDEITRTGIQSLKVRFNSVFGYYIEVTKANLDRVPPEYTRKQTIANGERFITPELKEM
                     EGKILGAEERGVKLEYELFQRMREAVLGRLAEIQQTAAALAQLDVLDSFAETARLYTY
                     CRPKIGSEGVLFIRDGRHPVLEQNLSEERFVPNDARLGGETQIALITGPNMAGKSTYI
                     RQVALLTLLAQTGSFIPAAEARIDLVDRIFTRIGASDDLTRGQSTFMVEMCETANILN
                     NATPRSLIILDEIGRGTSTFDGLSLAWSIVEHLHNQVGAKTLFATHYHELTELAARLP
                     RLRNFNVAVREWHDQIVFLRKIVEGGTDKSYGIQVARLAGVPKSVLERAKEILGNLEE
                     SELTPEGNVRQAARRQQDRDKLKQLAPPPQMDLFG"
     gene            1410..1859
                     /locus_tag="VIL39_00150"
     CDS             1410..1859
                     /locus_tag="VIL39_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5479866.1"
                     /translation="MKITEALVAEHTIFLGVFDQIERVLPSLTTPMEVVTMARIIEGL
                     LETHAQTETNLAYSALDHVLEHNGELKRMHQDHHEIDARLREVHTAATVAQARRLLKT
                     AITTSREHFRAEERSVFPLLEKVLQEETLIELGQNWLQRKPAPAPRA"
     gene            complement(1887..2405)
                     /locus_tag="VIL39_00155"
     CDS             complement(1887..2405)
                     /locus_tag="VIL39_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5479867.1"
                     /translation="MDEKLGFIFGRRSIRAYSPAAVSEAAVQKLLEAAMAAPSAAGKD
                     PWRFVVIHNRGKLAELAAALPNGKMLAGAGLGIAVCGDLQAAHDQQLSYLLQDCSAAI
                     ENLLLCAHVLGLGACWLGVHPREQRMSDLRQLLSLPPSVIPVACIAIGHPAEAKEPRT
                     RFKQNYVHPETW"
     gene            2863..3048
                     /locus_tag="VIL39_00160"
     CDS             2863..3048
                     /locus_tag="VIL39_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479868.1"
                     /translation="MPTTCCLHRAYTLLTRWQQAEWWLAVPGLEGWGTDGRRKRTQKF
                     RPGPEPPPSNTFRVRLD"
     gene            complement(3130..>5419)
                     /locus_tag="VIL39_00165"
     CDS             complement(3130..>5419)
                     /locus_tag="VIL39_00165"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479869.1"
                     /translation="SAGGVSPPAQTPGGTPGKLAGGTPAPHSAAALNLRSILNRALGP
                     GHVHGMDLWWDGSRVVFGYARTSSDEPPAGWLDRARSYTFRRTVEPTHLYEIGIDGKN
                     LRQLTDGEWSDLDPTYTPGGDIVFVSERCGTSLQCNEYDKDETSCNLYVMKPDGTGIR
                     RLSASKDGDYLPHTLDDGTVAYLRWEYHERSWAFIQSIWTIRPDGTGADALFKQHFVN
                     PWSLEDARSIPGSKKLVCIAAGHHTLAAGPLVVVDPSLGINEPKGISIVTPDVKPPEG
                     GMDGVRTPEGGTQDGGGLYSTPWALSEKCFLVSYTYSNKETDAKGYALYVIDVFGNKE
                     LIHRDPAISCFVPMPMGSRPRPPVFPEFVDLSKDRATCLISDVSFGSEAIADRIRYVR
                     IAEPIGWPYDNQLGGQRYGEKGPHLINWTPIRILGDVPVERDGSAHFEVPADTAVYFQ
                     LLDENRMELRRMRSFISFQPGETRACAGCHETRGLSPRPHAAPLAASLPPRALIPPPW
                     GNRPVSFLRDIQPILDRHCVQCHSGLKPAGGLDFCGGLTDWSREYEQAWGLVPGYGFN
                     RAFETITRAKLVATAEPNLQDASVTPPLAYGAHASKLFKALDSPAQRKRGRLTADELL
                     RMTMWMDANAPYHDRFVNKRANEKVYDIATDKELARQIAAVHERRCAACHKAAEVTRL
                     DWIDLRQPERTLFLRAPLNKTAGGSQSCQGTVYNDATDPDYQSLRELVAAAVRKAWAF
                     PRRDLQSLTDERLAAASPPHAP"
BASE COUNT         1039 a         1602 c         1814 g          964 t
ORIGIN      
        1 gggcgggcga ttgtggatga gccaccgctg gcggtgaagg agggcggcat gattcgcgac
       61 ggcttcgacc ccgcgctgga cgagctgcgc acggcgatgc gcggaggcaa ggactggatc
      121 gccaaattgc agcaggatga aatcacgcgc accggcatcc aatcgctcaa ggtgcgattc
      181 aactcggtgt tcggctatta catcgaggtg acgaaggcga acctggaccg cgtgccgccg
      241 gaatataccc gcaagcagac cattgccaac ggcgagcggt tcatcacgcc agagttgaag
      301 gagatggagg gaaagatcct gggcgcggag gagcggggcg tgaagctgga gtatgagttg
      361 ttccaacgga tgcgcgaggc cgtgctcggg cggctggcag aaattcaaca gaccgcggca
      421 gcgctggcac aactggacgt gctggattca tttgccgaaa cggcgcgttt gtatacttat
      481 tgccgcccga agatcggcag cgaaggggtc ctgtttatcc gcgatggtcg gcacccggtg
      541 ctggaacaga acttgagcga ggagcgattt gtgcccaatg acgcgcggtt gggcggcgag
      601 acgcaaatcg cgctcatcac ggggccaaac atggcgggca agagcactta catacgccag
      661 gtggcgctgc tcacgctgct ggcgcagaca gggtcgttca tcccggcagc ggaggcgcgc
      721 attgacctgg tggaccgcat ctttacgcgc attggggcaa gcgatgatct gacgcgtgga
      781 caatccacgt tcatggtgga gatgtgcgag acggcgaaca tcctcaacaa tgcgacgccg
      841 cgcagcctga tcatcctgga tgaaattggc cgaggcacga gcacgttcga cgggctgagc
      901 ctggcgtgga gcattgtcga gcacttgcac aaccaggtgg gcgcgaagac gctgtttgcg
      961 acgcattatc acgagttaac cgaactggcg gcgcgtttgc cgcggctgcg gaacttcaac
     1021 gtcgccgtgc gcgagtggca cgaccagatt gtgtttctgc ggaagattgt agaaggcggg
     1081 acggacaaga gctacggcat ccaggtggcc cgcctcgcgg gcgtgcccaa gagcgtcctg
     1141 gagcgagcca aggagatcct gggaaacctg gaggaatccg aactcacgcc cgagggcaat
     1201 gtgcgccaag ccgcgcggcg gcagcaggac cgcgacaagc tcaagcaact ggcgccgccc
     1261 ccgcagatgg atttgttcgg ttagcggcgg caccccggtc ccttcaagcc ggaaacaacg
     1321 gaacactctt gcatctttgg agcggcccgg ccaaccgcgc aactcgccgg ttgcggcttt
     1381 gtcagagttc agttaagata atgcacaata tgaagattac cgaagctctg gtcgcggagc
     1441 atactatatt cctgggtgtg ttcgatcaaa tcgagcgcgt tttgcccagc ctcaccacac
     1501 cgatggaggt ggtgacgatg gcccgcatca tagaggggtt gctggagacc cacgcccaga
     1561 cggaaaccaa ccttgcgtat tcggccctgg atcatgtgct ggagcacaac ggcgagctta
     1621 agcgcatgca ccaggatcat catgagattg atgctcggtt gcgagaagtg cacacagcgg
     1681 ccactgtcgc ccaggcgcgg cgcctgttga agaccgcgat cacgacctcg cgcgagcact
     1741 ttcgcgcgga agagcgcagc gtgtttccgc tgctcgagaa ggtgctccag gaggaaacgc
     1801 ttatcgaatt agggcagaac tggctgcaac gaaaaccggc cccggcgccc cgcgcttagc
     1861 ctcagttccc tggcggagca cagagctcac caggtctcgg gatgcacgta gttctgcttg
     1921 aatcttgtgc gcggttcctt tgcctcggcc gggtggccga tggcgatgca ggcgaccgga
     1981 atcacggaag gcggcagtga gagaagctgt ctgaggtcgc tcatccgctg ttccctggga
     2041 tggacgccca accagcaggc tcccaagccg aggacatgcg cgcacaagag cagattctca
     2101 atggctgccg agcaatcctg gagcaggtag ctgagctgct gatcgtgggc cgcctgtaga
     2161 tcgccgcaga ccgcaatgcc caaccccgca ccggcgagca tcttgccgtt cggcaaggcg
     2221 gcggccaact ctgcgagctt tccccggtta tggatcacca caaaccgcca cgggtccttg
     2281 ccggcagcgg aaggggccgc catcgcggcc tccagcagct tttggacagc cgcctcgctc
     2341 actgccgcag gggagtaagc gcggatgctc cttcggccaa aaataaaccc aagcttctcg
     2401 tccatattcg cattcataaa ggtctatgtg ggggaagaat atccagcctc ctgccggtca
     2461 atgcaagcat tcccgaacca gcaaagttcc gacggacccc atacacccca catttagccg
     2521 gcgtatgtct tggcatggtc tatgcgcaca ttcatgggaa cggaacaaga tgatgtcaca
     2581 atgccacggc ccacagtcgg gtgaattaca aacaatccgc acgtgaattc gcgggtgaga
     2641 cctcggcacc ggccccagag agaccgtctg gggcttcctt ggttgtaccg gtccatgctc
     2701 agtccatgct gagtccgcgc ttgcatcgcc aggctacctg agaagggctg tggtgtgcgg
     2761 agtgcgggat gcaccgtggg actggggagg tgggaggcgg aagaaaccca gcaaaaccat
     2821 gcgaagacca tgcgaagacc atgcgaagac tatgcgaaga ctatgccaac aacatgctgc
     2881 ttgcaccgtg cttacacgct gcttacacgc tggcagcagg ccgagtggtg gctggcagtg
     2941 ccggggctgg aggggtgggg cacggacgga cggaggaagc ggacgcagaa gttccggcct
     3001 ggccctgagc cgcccccgag caataccttc cgcgtccgtc tggattgagt caacgcgccg
     3061 ccgctcgaat cgcccgtccg cgcaacttct ccataagaag tcggctcaac cgcaggcgga
     3121 tcgtccgcct cagggtgcgt gaggcgggga agccgcagcc aatctttcgt cagtcaggct
     3181 ttggagatcg cggcggggga atgcccaggc cttcctgacg gcggcggcaa ccagttcgcg
     3241 cagtgactgg taatccggat cggtggcgtc gttgtaaacc gtgccctggc agctttggct
     3301 tccgccagcc gtcttgttga gcggtgcgcg caggaaaagc gtgcgctccg gttggcgcag
     3361 gtcaatccaa tccaggcggg tcacttcggc ggccttgtgg caggcggcgc aacggcgttc
     3421 atgcaccgca gcgatctgcc gggccagttc cttgtcggtg gcgatgtcgt agaccttctc
     3481 gttggcccgc ttgttgacga agcgatcgtg gtaaggcgca ttggcgtcca tccacatcgt
     3541 catccgcagc agctcgtcag cagtcaatcg gccgcgcttg cgttgggccg ggctatccag
     3601 cgccttgaac agtttgctcg cgtgcgctcc gtaggccagg ggcggggtga cactggcatc
     3661 ctggagattg ggttctgccg tggcgaccag cttggcccgg gtgatcgtct caaaggcgcg
     3721 gttgaagccg taaccgggca ccaggcccca cgcctgctcg tactcgcggg accagtcggt
     3781 caggccgccg cagaaatcca gtccgcccgc cggcttgaga ccgctatggc actggacgca
     3841 atgccggtcg aggatcggct ggatgtcgcg caggaagctc accgggcggt tgccccaggg
     3901 cggcggaatg agcgcgcggg gcggaagcga ggcggccagc ggcgccgcgt gaggacgcgg
     3961 cgacaggccg cgcgtttcgt ggcagccggc acaggcgcgg gtttcgcctg gctgaaagct
     4021 gatgaagctt ctcatgcggc gcagttccat gcggttctcg tccagcaact ggaaatagac
     4081 ggcggtgtcg gccggcactt cgaagtgcgc gctgccatcc cgctcgaccg gcacgtcgcc
     4141 gagaatgcgg atgggggtcc agttgatcag gtgcgggcct ttctcgccat agcgctgccc
     4201 gccgagctgg ttgtcgtagg gccagccaat cggctcggcg atgcggacat agcggatgcg
     4261 gtcggcgatt gcctccgagc cgaagctcac atcgctgatg aggcaggtgg cgcggtcctt
     4321 cgacaagtcc acgaattcag gaaacaccgg cgggcgcggc cgggacccca tcggcatcgg
     4381 cacgaagcag gaaatggccg ggtcgcgatg gatgagttcc ttgttgccaa agacatcgat
     4441 cacgtagagg gcgtaaccct tggcgtcggt ttccttgttt gagtaagtgt aagaaaccag
     4501 gaagcacttc tcggaaagcg cccagggcgt cgaatagagc ccgccgccgt cttgcgtgcc
     4561 gccctcgggc gtgcgcacgc cgtccatgcc gccttcgggc ggcttcacgt caggcgtgac
     4621 gatggatatg cctttgggtt cgttgatgcc aagggacggg tccaccacca ccagcggccc
     4681 ggccgccaag gtgtggtggc cggcagcgat gcaaacgagc ttcttgctgc cggggatcga
     4741 gcgggcgtct tcgagggacc agggattgac gaagtgctgc ttgaagaggg catccgcgcc
     4801 ggtgccgtca ggccggatgg tccagatgga ttggatgaag gcccaactgc gctcgtgata
     4861 ttcccagcgg agataggcca cggtgccgtc gtccagcgtg tggggcaggt aatcgccgtc
     4921 cttgctcgcg ctcaggcggc ggatgccggt gccgtcgggc ttcatcacgt agaggttgca
     4981 gcttgtttca tccttgtcgt attcattgca ctggagcgat gtgccgcacc gctcggaaac
     5041 gaagacaatg tcgccgcccg gcgtgtaagt cgggtccagg tcactccact cgccgtcggt
     5101 gagttggcgc agattctttc catcaatgcc gatctcgtag aggtgggtgg gctcaactgt
     5161 ccgccggaaa gtatagcttc gcgcgcggtc gagccagcct gcgggcggct cgtcgctcga
     5221 tgtgcgcgca tagccgaaca cgacgcggct gccatcccac cataggtcca taccatgcac
     5281 gtgaccgggc cccagcgcgc ggttgaggat ggaacggaga ttcaaggcgg ctgctgaatg
     5341 tggcgcgggc gtcccgcctg cgagtttccc gggcgtcccg cccggggtct gtgctggcgg
     5401 cgagacgccg ccggcactc
//
LOCUS       DATCGS010000011         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_644495, whole genome shotgun sequence.
ACCESSION   DATCGS010000011 DATCGS010000000
VERSION     DATCGS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644495"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VIL39_00170"
     CDS             complement(<1..1184)
                     /locus_tag="VIL39_00170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5479870.1"
                     /translation="MTLNRRAFLKQSGVGIAGLGLLSFVPGCRTSRQTAGTGKLPRST
                     PEAEGVSSASILAFLKAVRHSKHELHSFMLARHGRVVAEGWWSPYGPEFNHGLYSLSK
                     SFTSTAVGFAVAEGKLRVDDRVISFFPHDLPEQVSEHLAALRVKDLLTMSAGNGKEPT
                     STMVDQANWVKAFLACPIPNTPGTQFMYNSSATYMLSAIVQQVTGQKVLDYLQPRLFE
                     PLSIQGTRWETCPRDINVGGWGLSIQTEGLVKFGQLYLQKGVWQGRQILPPQWVEEAT
                     TFKIQQPAPARPGRPNDQNDWLQGYCYQFWRCRHNAFRGDGAFGQYMIVMPDQDAVVA
                     ITTETSDMPGELDLVWEHLLPAMKPQPLPRDPQPQAELRQTLSSLALTPSTGQSASPL
                     TGR"
     gene            complement(1580..>3515)
                     /locus_tag="VIL39_00175"
     CDS             complement(1580..>3515)
                     /locus_tag="VIL39_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5479871.1"
                     /translation="RRIYEDIVRKLKDPALLEYAGRDVFKVRIFPIEPNSKKRITLSY
                     TELLKADNGLVSYLLPLSTEKFSAKPIRNVSVKVDLESKRPLKSIYSPSHSVQVKRQG
                     PNRAVAGYEAEDVQPDTDFALYYAPEKDEIGVNLLAQRQSDEDGYFLLLASPGVDVKE
                     QQVVMKDIAFVLDTSGSMAGKKLEQAKKALQFCVENLNDGDRFEIIRFSTEVEPLFDK
                     LVKATEPNRARANDFIRELKPIGGTAIDDALRKALALRPGQDDRPFVVIFLTDGRPTI
                     GTTDEDQIVANVKKENEGRTRVFCFGIGTDVNTHLLDRITEETRAVSQYVLPEEDLEV
                     KVSAFFSKIKEPVLANPTLTFTGGIRATKLYPSPLPDLFKGEQLVLVGRYTGKGDAAV
                     IVEGTVNGAARKFTYEVNFPGEASESEFIPRLWATRRVGYLLDEIRLHGENSELRDEV
                     TELARKYSIVTPYTAYLILKDETQRNVPMRMRSLQSFDGDRGARTEAAKAWSEFKDVR
                     GGEQAVANARYGMAFKAADAAAPAGVSGALESRRALGLSAGSGRAPAASPTDKSKERL
                     VEYSQQAQFVAGKSFFQNDKQWIDSAVQKHPNMKRVRIQFGSTEYFDLAAKNAKALPW
                     LALGQNVQFVLDNTVYDIYE"
BASE COUNT          623 a         1106 c         1053 g          733 t
ORIGIN      
        1 cgccctgtca gaggcgaagc ggactgccct gttgatggcg tcagggccag tgaagagagc
       61 gtttgccgca gttccgcttg tggctgcggg tcgcggggca gaggctgtgg tttcatcgct
      121 ggcagcaaat gctcccagac caggtccaac tcgcctggca tgtcgctggt ttcagtggta
      181 atcgcgacca ccgcgtcctg atccggcatc acgatcatgt actgtccgaa ggcgccgtca
      241 ccgcggaacg cgttatgccg gcagcgccag aactggtagc aatagccctg caaccagtcg
      301 ttctggtcgt tgggccgacc cggcctggcg ggagcaggct gctggatctt gaaagtggtc
      361 gcctcttcca cccattgggg agggaggatc tgccggcctt gccaaacgcc cttttgcaga
      421 tacaactggc cgaacttcac gaggccctcg gtttgaatgc tcaagcccca gccgccaaca
      481 ttgatgtctc gcggacaggt ttcccagcgt gtgccttgga tgcttaacgg ttcgaacaga
      541 cggggctgca ggtaatcaag gaccttctgc cccgtgacct gttgaacgat agccgagagc
      601 atatatgtgg cgctgctgtt atacatgaac tgcgtgcccg gcgtattagg gatagggcag
      661 gccaggaagg ctttcaccca gttcgcttgg tccaccattg tcgaggtcgg ttccttcccg
      721 ttgccagccg acatcgtcag caggtccttc acgcgcaacg ccgccagatg ctcgcttacc
      781 tgttcaggca ggtcgtgtgg aaagaacgag atcacccggt catccacccg gagcttgcct
      841 tctgccacgg cgaaacccac cgcggtagag gtaaagctct tgctcagcga atacagtccg
      901 tgattgaact ctggtccgta gggcgaccac cagccttcgg ctacaacccg gccgtggcgg
      961 gccagcatga agctatgcaa ctcatgtttg ctgtgccgga ccgctttcag gaacgccagg
     1021 atgcttgccg aggatacgcc ttcagcttcc ggcgtgctgc gcgggagctt gccggtgccg
     1081 gccgtttgtc ggctcgtccg gcaaccagga acaaacgaaa gcagtcccag tcccgctatc
     1141 ccgactccgc tctgtttgag aaacgctctg cgattcagtg tcatgccttt ggcattcaat
     1201 cacaactccc ccctcaatct caagccatcc ttgaagtctg tccttccgga agcgcgcacc
     1261 ttcctgttcg tcgcaccatc ttggcgacgg ttggcgggca agagcgaagc taagagtcag
     1321 cttgcgtgca gcctgagcga ccaccgcagc gtctatacga aaagcagagg gaagcctgcg
     1381 caaggggaca gaaaaccctg attcccttcg taactccttc gtaacccctt cgaagcccct
     1441 tcgaagccct ttcgtgacaa cacgcggaca acacctgagc tgcgggatag ccgctggctt
     1501 gcagtgcgct gtgagcggca caccccacct ccagtttgcc ccgtggtggc gcacgcaagc
     1561 tgcgccccat ccggatattt tactcgtaga tgtcatagac cgtgttatcc aggacgaact
     1621 gcacgttctg ccccaacgcc agccagggca gcgcctttgc attcttagcg gccaggtcga
     1681 agtattccgt cgagccgaac tggatgcgca cgcgtttcat gttcggatgc ttctggacgg
     1741 cggagtcaat ccattgcttg tcgttctgga agaagctctt gccggcgacg aactgcgcct
     1801 gctgcgaata ctcaacaagc cgctccttgc tcttgtcggt cggcgatgcc gcgggtgcgc
     1861 gacctgaccc ggcagagagc ccgagcgccc gccgcgattc gagggcgccg ctgacccccg
     1921 cgggcgccgc tgcatccgct gctttgaagg ccatgccgta ccgggcgttc gccacggcct
     1981 gctcgccgcc acgcacatct ttgaactcgc tccaagcctt cgccgcttcc gtgcgcgcgc
     2041 cgcggtcgcc gtcaaagctc tgcaaggaac gcatccgcat cggcacattg cgctgggttt
     2101 cgtctttcag gatcaggtaa gcggtgtagg gcgtcacgat gctgtacttg cgcgccagct
     2161 cggtcacctc gtcgcgcagc tcggagttct ctccatgcag ccggatttcg tccaacagat
     2221 agccgacgcg ccgcgtcgcc cagaggcggg ggatgaactc gctttccgac gcctcacccg
     2281 ggaagttcac ttcgtaggta aacttcctgg ccgcgccgtt cacggtgcct tcgacaatta
     2341 cagccgcatc gcctttgccg gtgtagcgtc ccaccaatac cagttgttcg cccttgaaca
     2401 ggtcgggcag cggcgagggg tagagcttcg ttgcgcggat accgcccgtg aacgttagcg
     2461 tcgggttcgc caggaccggc tccttgatct tcgagaagaa ggccgagacc ttcacctcca
     2521 ggtcttcctc gggcaggacg tactggctga cggcacgggt ctcctcggta atgcggtcga
     2581 gcaggtgcgt gttcacgtcc gtgccgatgc cgaagcagaa gacgcgcgtg cggccctcgt
     2641 tctccttctt cacgttcgcc acgatctggt cctcgtcggt ggtgccgatg gtggggcggc
     2701 catcggtgag gaagatgacc acgaaggggc ggtcgtcctg gcctggccgc aaagccaatg
     2761 ccttgcgtag cgcgtcgtca atcgccgtgc cgccgatggg cttgagctcg cggatgaaat
     2821 cgttggccct ggcgcggttc ggttcggtgg ccttgacgag cttgtcgaag agcggctcga
     2881 cctcggtgga gaagcggatg atctcgaagc ggtcaccgtc gttgaggttc tcgacacaga
     2941 attgcagggc cttcttggcc tgctctagct tcttgccggc catggagccg gaggtgtcca
     3001 gcacaaatgc aatatccttc atcacgacct gctgttcctt cacgtccaca cccggggaag
     3061 cgagcagcag aaaatagcca tcctcatcgc tctggcgctg agccagcagg ttgacgccaa
     3121 tctcatcctt ctccggcgcg taatagagcg cgaagtccgt gtcgggctgg acatcttcgg
     3181 cctcataccc ggcgaccgcc cggtttggcc cctggcgctt cacctggacc gaatggctcg
     3241 gcgagtaaat ggacttgagc gggcgtttgc tctccaggtc caccttcacg ctcacgttcc
     3301 ggatcggctt cgcggagaac ttctccgtgc tgagcggcag caggtagctg acgaggccat
     3361 tgtccgcctt gagcagttcg gtgtaggaca gggtgatgcg cttcttgctg tttggctcga
     3421 tgggaaagat gcgcaccttg aacacgtcgc gccccgcata ttcgagcagc gccgggtcct
     3481 tgagcttgcg gacgatgtcc tcgtaaatcc gccgc
//
LOCUS       DATCGS010000012         5237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_496194, whole genome shotgun sequence.
ACCESSION   DATCGS010000012 DATCGS010000000
VERSION     DATCGS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496194"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1198
                     /locus_tag="VIL39_00180"
     CDS             308..1198
                     /locus_tag="VIL39_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5479872.1"
                     /translation="MKYFIQLFLSAACATHMLAAESKIIAPGATLQKLAGDFVFTEGP
                     ACDRQGNVFFTDQPNDRIHKWSIDGKLSTFMHPCGRANGLSFDHEGYLWACADEKNEL
                     WRIDPAGKAAVVVKDYKGKLLNGPNDVWIQPTGGLYFTDPYYKRDYWKRGPKEMDECV
                     YYLAPDHQTLTRVIEDLKQPNGIIGTPDGKLLYVADIGAGKTYRYSIQPDGALKEKKL
                     FCEMGSDGMTIDSAGNIYLTGKGVSVFDSSGKKIEHIEVPENWTANVCFGGKDKQTLF
                     ITATKSLYGLHMRVSGVDSQ"
     gene            complement(1238..2353)
                     /locus_tag="VIL39_00185"
     CDS             complement(1238..2353)
                     /locus_tag="VIL39_00185"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5479873.1"
                     /translation="MKHRDIVPRLLAWFAQNARDLPWRRTRDPYAIWVSEVMLQQTQV
                     KTVLPYWERWMRALPNLAALAKAKPQTLHKLWEGLGYYTRVRNLQRAAQLILAQPGGR
                     FPHDFEGVIALPGVGRYTAGAICSIAFNQPRPILDGNVIRVLTRSYGIAGNPRERSVN
                     ARLWQLAEELVLQAAETAPLKRPSLHASRITHHASRPCAQFNESLMELGALVCTPRQP
                     RCGACPIVKHCVAHQEDRVHELPGLSRPIPVTPQRFIAFVAGRGGRFLVRQRPAGVVN
                     AHLWEFPNLELPPGNSDLKRAARSVLGVRPETLELLGTIKHSITRYRITLEAYRVTSR
                     QAAGIPATKGHWFGRGRLHQLPFTSAHKQILQRLGSR"
     gene            2542..3522
                     /gene="rnhC"
                     /locus_tag="VIL39_00190"
     CDS             2542..3522
                     /gene="rnhC"
                     /locus_tag="VIL39_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEY5479874.1"
                     /translation="MKPLTSYTSKLTDAQATALRTWLQTGNYRFREVPYARFAAEKEK
                     TNVVFYESGKLVVQGKGTQEFVEFVLEPQILQEARLGYEAVLNPDLLLPRLGVDESGK
                     GDFFGPLCIAGAYVNESVVKAWADVGIRDSKNISSDKRIRELAELIRKTPGCVTTVVP
                     IGNEAYNRLYAKMRSVNTLLAWGHARVIENLMGQRHRMNPPPVRAISDQFAANKETVA
                     KALMTMGREIELVQKHRAEEDLAVAAASILARHEFVTRLAALEKQFGTKLPKGASAAV
                     DAAAKEIVAREGPDGLAKVAKMHFRTALRAQGLPEPPKVEWRRRASPGKN"
     gene            complement(3983..4942)
                     /locus_tag="VIL39_00195"
     CDS             complement(3983..4942)
                     /locus_tag="VIL39_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEY5479875.1"
                     /translation="MKLRNSSPVRRSAKVTLRLVLTSLILVLPGRLLAASEERFTSPQ
                     TAVDALKAAVEARDTNVLQTIFGPAGHGLVSVDVVEASEERDLFMSRVREKINLVAAS
                     DSKQELQLGGDGWPFPIPLIKQNGQWFFDVEAGKEEILNRRIGANEMGAIQVCRAYMQ
                     AQREYASADRNADEVLEYAQHLRSTPGAHDGLYWSTRTVDELSPLGPLVAQARVEGYR
                     KQTKILTEEQSPYHGYYFQILTRQGKHAPGGKYNYIINGHMIAGFALVAWPAEWGNTG
                     VMTFLVNQRGKVYQKNLGPKTSSIAKAMTTYDPDNTWTLTREE"
BASE COUNT         1027 a         1580 c         1628 g         1002 t
ORIGIN      
        1 tgtcaacgga gaccattcga cagggctgcc ttcgagtccg gccggcggag gcacgatcca
       61 agcaggccgc cctgaaaccc gagggaaagg tcccgaatca gccgccgccc tggcaaggca
      121 gccgcctgcc ggatcaagcc cctttcggat ttcggttttc ggccttcctt cgagcctcgg
      181 gtttcgagtt tcggatttgg gccggctccg accaccattg gcccacccct ggaacatcct
      241 gccggcctct cctttctctc tttacaccaa accgaatttc gacttcgatc agggcagtta
      301 gactcaaatg aaatacttca tccaactctt cctgagtgcc gcttgcgcca cacacatgct
      361 ggcggcggag agcaagatca tcgccccggg cgcgacgctg cagaagctgg cgggcgattt
      421 cgtcttcacc gaagggccgg cctgcgaccg ccaaggcaat gtgttcttca ccgaccaacc
      481 caacgatcgc attcataaat ggagcattga cggcaagctc tcgacgttca tgcacccgtg
      541 cgggcgtgcc aacggccttt ccttcgatca cgagggctat ctctgggcct gcgccgatga
      601 aaagaacgag ctgtggcgca ttgatcccgc cggcaaggca gccgtggtcg tgaaggacta
      661 caaaggcaag ctgctcaatg ggccgaacga tgtgtggatc cagcccaccg gcgggttgta
      721 tttcacggac ccttactaca agcgagatta ttggaagcgc ggccccaaag agatggacga
      781 atgcgtgtat taccttgcgc cggatcacca gacgctgact cgcgttatcg aagacctgaa
      841 gcagccgaat ggcatcatcg gcacgccaga cggcaagttg ctctacgtgg ccgacatcgg
      901 cgccgggaag acctaccgct acagtatcca gccggatggc gcgctcaaag agaagaagct
      961 gttctgcgaa atgggctcgg acgggatgac gattgacagc gcaggcaata tctatctcac
     1021 gggcaaaggg gtgtcagtct tcgattcctc cggcaagaag atcgagcaca tcgaggtgcc
     1081 ggagaactgg accgcgaacg tgtgctttgg gggcaaagac aagcagacgc tgttcatcac
     1141 cgccaccaaa agcctttacg ggctgcacat gagggtcagc ggcgttgata gccagtaggg
     1201 aaaaccatgt agcaaaccaa ctgctctggc gctgacccta ccggctgccg agacgttgca
     1261 ggatttgctt gtgagcgctg gtgaacggca actggtgcaa tcggccccgc ccaaaccaat
     1321 ggcctttggt tgccgggatt ccggctgcct gacgactcgt gacccggtac gcctccaaag
     1381 taatgcgata gcgcgtgatg gagtgcttga tcgtgccgag caactcaagc gtctccggcc
     1441 gcactcccag gacgctgcgg gccgcccgct tcaagtcgga attaccgggg ggcagttcca
     1501 ggttggggaa ttcccagagg tgcgcgttca ccacgccggc gggtctttgg cggacgagga
     1561 agcggccgcc tctccctgcg acgaaggcaa taaagcgctg cggcgtgacg gggatgggcc
     1621 gactgaggcc gggcagttca tggacgcggt cctcctgatg cgccacgcaa tgcttcacga
     1681 tcggacaggc accgcatcgg ggctgccggg gcgtgcagac gagcgcgccg agttccatga
     1741 gcgactcgtt gaactgggcg cacggacgtg atgcgtgatg cgtgatgcgt gatgcgtgaa
     1801 gtgagggacg tttgagtggg gcagtttcag cggcttggag caccaactct tccgcaagct
     1861 gccataggcg ggcgttgacg cttcgctcgc gcggatttcc cgcaatccca tacgagcgtg
     1921 tcaggacccg aatcacattg ccgtccagga tcggtcgcgg ctggttgaag gcgatgctgc
     1981 agatggcacc ggcggtgtaa cgcccgacac ccggcagcgc gatcacaccc tcgaagtcgt
     2041 gcgggaagcg gcccccaggt tgcgccagga tgagttgcgc ggcgcgttgc aggtttcgca
     2101 cgcgagtgta atagccgagt ccttcccaga gtttatgcag cgtttgcggt ttggctttgg
     2161 ccagcgccgc gaggttcggc agtgcccgca tccagcgctc ccaatagggc aggaccgttt
     2221 tgacctgggt ttgctggagc atgacttccg agacccagat agcgtatgga tcgcgggtgc
     2281 ggcgccaagg caggtcccgc gcattctgag cgaaccaagc cagcagtctc ggcacaatgt
     2341 ctcggtgttt catgcggtcg caatgagctt aaatccgaat tgcgaattcc gaaacccgaa
     2401 tcggaacccg cgcctgctta tgccaccccc tgccccattc gcatgtcacg catcacgaat
     2461 cacgcatcac gagccacccc ttcccgccca ttcccgccgc ttgccgttct cgcgccaact
     2521 ctctacgata tgacgacctt tgtgaaacct ttaacgtcct ataccagcaa gctcaccgac
     2581 gcccaggcaa ccgctctccg cacctggttg cagacgggta actaccgctt tcgcgaggtc
     2641 ccgtacgcgc ggttcgctgc cgagaaagag aagaccaatg tggtgtttta cgagagcggc
     2701 aagctggtgg tgcagggcaa agggacgcag gagttcgtgg agtttgtgct cgagccacag
     2761 atcctgcagg aagcgcggct cggctacgag gcggtgttga atcccgacct gctgctgcct
     2821 cgcctgggcg tggacgagag cggcaagggc gatttcttcg gtccgctctg catcgcgggc
     2881 gcttacgtca acgaatcggt ggtcaaggcc tgggccgacg tgggcatccg cgactcgaag
     2941 aacatctcca gcgacaagcg aattagggag ctagcggaat tgattcgcaa gacgcccggt
     3001 tgcgtgacca cggttgtccc catcggcaac gaagcctaca atcgccttta cgccaagatg
     3061 aggagcgtca acaccctgct tgcttggggg catgcgcggg taatcgagaa cctgatgggc
     3121 cagcgccatc ggatgaatcc gccgccggtg cgggccatca gcgaccagtt tgccgccaat
     3181 aaggaaaccg tcgccaaggc gctgatgact atgggccgcg agatcgaact ggtccagaag
     3241 caccgggccg aagaagacct ggccgtggcc gcggcttcaa tcctggcccg gcacgaattc
     3301 gtcactcgcc tggccgcttt ggaaaagcaa ttcggcacaa aactcccaaa aggtgcctcg
     3361 gcggccgtgg atgcagcagc caaggaaatc gtcgcccggg agggtcctga tggcctcgcc
     3421 aaggttgcca agatgcactt ccgcaccgcc ctccgggcgc aaggactacc agagccaccg
     3481 aaggtcgaat ggcgacgccg ggccagcccc ggcaagaact gatgccgacc cgcccaacgg
     3541 tcggatggcc ctgagccgcc caacgcgctc ctgcgcgact gacgactgaa gtttcccctt
     3601 ctccaaacca gcccttacgg cgttgggtcc aggccagaaa cgtgccggca tagccgtctg
     3661 agctaacaga gcttttgcgc ggtcaagctg gacgcgaagg atttcgtggc ttggcgaacg
     3721 tccgcaggca tcgcggaagc gctggtagaa agtgctgtgc gagatcccaa caggttgcag
     3781 cagatcctcc acactgagtc cgtggcaagc ctctttccgg atcagacata tcgcgttggc
     3841 aatgaggcgg tgctggacag cagtggcctc ggtagagcgg cgagcggcca ccccgagggg
     3901 cgcgaagagg agcggcgaaa ttccgggccg acgatgtgcg ttccctcatt cccgtaaaga
     3961 gcggtgcttc cgattcccga cttcattcct ctctcgtcag tgtccaggta ttgtccgggt
     4021 cgtaggtggt catcgccttg gcgattgagg aagtcttagg accgaggttc ttctgataga
     4081 ccttgcctcg ctggttgacg aggaaagtca tcacgccggt gttgccccac tccgccggcc
     4141 aggcgaccaa cgcaaagccc gctatcatgt gcccgttgat gatgtagttg tacttgccgc
     4201 cgggcgcgtg tttgccctgt cgggtcagga tctggaaata gtaaccatga tagggcgact
     4261 gctcttccgt caggatcttg gtctgtttcc gataaccttc tacccgggct tgcgcgacca
     4321 gcgggcctaa agggctgagt tcatcgaccg ttcgcgtgga ccagtagagg ccgtcgtggg
     4381 cgccgggcgt gctgcgaaga tgctgggcgt attcgagcac ttcatccgcg ttgcgatccg
     4441 cgctggcata ttcgcgctgg gcctgcatgt aagcgcggca gacctgaata gcgcccatct
     4501 cattggcccc aatgcgccgg ttgagaatct cctctttgcc cgcctcgaca tcgaagaacc
     4561 attgcccgtt ctgtttgatc aggggaatgg gaaagggcca gccgtcgccg cccagttgca
     4621 actcttgctt ggagtcggac gcggccacca gattaatctt ctccctgacg cggctcatga
     4681 acaaatcccg ctcctcagac gcctcgacga catcgactga taccaggccg tgcccggcag
     4741 gaccgaaaat cgtttgcaag acattggtat ctctggcctc aactgccgct ttgagcgcgt
     4801 ccactgcggt ttgcggagag gtaaatcgct cctcactcgc ggccagcagg cgccctggca
     4861 gcaccaggat gagggaagtg agcaccagtc tcagcgtaac cttggcggac cggcgaacag
     4921 gtgaagaatt gcggagtttc atcggctgtt gttctttcgt gaaatcgtta ttggaccaag
     4981 ggatcagtgt tttccgccac cggatgggcg gctcggagcc gccgaatgcg cgggcggggt
     5041 tggatgggcg gcaggtgccg ccggtcggga tacagtcgtc tggcggcttt cctggccgcg
     5101 gctgctaaac tgctgggtct gatgcgcatt ctggacccca atcagcgcgc cgttggctgg
     5161 ccgcgagccg ggcgctttcg ccggccgggg ttggcccccg attacagtta ccgtggtgtg
     5221 gaccggtgcg gggctgg
//
LOCUS       DATCGS010000013         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_496248, whole genome shotgun sequence.
ACCESSION   DATCGS010000013 DATCGS010000000
VERSION     DATCGS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496248"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3416
                     /locus_tag="VIL39_00200"
     CDS             <1..3416
                     /locus_tag="VIL39_00200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5479876.1"
                     /translation="LYGRLHVLFRQAWKRRVSLRLVSLKLSNVYKGIWRTELALEARG
                     QRHDAQRRLAAVVDELRRAHGHEVLLRGHDFRLRQPALDSAASLRLKPQPSSSPVRLP
                     PLRKATSYVPLNIHSHFSFLDSTLSIPAIIELARKHELPAVALTDTGNLHGAVEFCLA
                     ARQAGIKPIVGAEVDVDGQPVWLYVENAIGYANLCRLLSQDQAEAAPTQSEADVCLRH
                     VALRARQLDGLMDGLLAVTRHTALASLFKGRCYHAVTSGDGGQREQDTWPGVLLQPVH
                     YAAPADRQKFDIVQSIRTRTLLRQAHPGKRLTDEFHFRPPGEVLAACREHRNLLDHAQ
                     EIADRCTFVLSPAPPQFPAFHPPDGSTAHAFLRKLVFEGVHRRYRERAEGLRPQVEEE
                     LAIIAEVGYEEYFLVVWDMLQACRRRGIDWITRGSAADSLVCYCLGISDVCPLRFDLY
                     FRRFLNKERMAMNKLPDIDMDFPHDRKDDVVDLLFEKYGREHTAVVGGFSTFQARSAV
                     ADVAKVLGVSEFQIRRFTEHFPWTSARAITEALAGRQECRDLPIQEEPYRTALAMAAF
                     LDGFPRYPKMHPCGVVLSRQPMPQLTPTFMSSKGYPTTHFDMDAVEAIGLVKIDILAQ
                     GGLAVMRDAKTWLRQRGVEVDLGRLEPWEDPEVWAMIASGSARAVHHIESPAMVGLCR
                     QCNVRNIDGLVAIVSVIRPGAANEGKKQAFARRYQGLEPVTYPHPSLEPCLKSTFGLV
                     VYEEHILQICEAFAGLPPGRADALRRALGKNKAPLIEEIRQEFIAAARARGHPDTKIA
                     EVWDLVGGFAGYAFCKAHSTAYGVEAYQSAWLKRYHPVEFMAAVLSNGKGFYDPLVYV
                     LECHRLGIPILPPTVNQPGPQFGVESQAIRVPLTRVQGLTQRTQEQMTGERQRGAFRS
                     LSDFYRRVVPSREEMEALLQVGAFDEFGQSRTAQFWEVQQLYHAFGGTSDSSQGWLLP
                     APETNRLPGVPLQEPTRRQRLKWETELLGFTASGHPLELYGDIAWDTYCPVKRLGENV
                     GQQVVTCGLIIEQRTHHQVTGEPMKFLTLADWTGMVETELFASTYRSYGLATIRYPVL
                     EVEATVEPFENGRGFSLRVLRAGRPRQKLSAVSNGRDRTQAVRASFQRE"
BASE COUNT          609 a         1097 c         1090 g          624 t
ORIGIN      
        1 atctgtatgg acgcctccat gttttgttcc ggcaggcttg gaagcggcgg gtcagcctgc
       61 ggctcgtctc gctcaagctc tcgaatgttt acaagggcat ctggcgcacg gagctggcgt
      121 tggaggcgcg aggccagcgc cacgacgccc agcgccgcct cgccgccgta gtggacgagc
      181 ttcgccgcgc ccacgggcac gaagttctcc tgcgcgggca tgatttccgg ttgcgccaac
      241 cggccctgga cagcgccgca tcgctgcgac tcaaaccaca gccctcatct tctcccgtcc
      301 gactgccgcc gctgagaaaa gccaccagct acgtgccgtt gaacatccac agtcacttct
      361 ccttcctcga ttcgactctc tccattcctg ccatcatcga gctggcccgg aaacacgaac
      421 tgcccgcggt ggcgcttacg gacacgggca accttcacgg cgcggtcgag ttctgtttgg
      481 cggccaggca ggcgggcatt aaacccatcg ttggcgcgga ggttgacgtg gatggccaac
      541 cggtctggct ctacgtcgag aacgccatcg gctacgccaa cctctgccgc ctgttgtcgc
      601 aagaccaagc cgaagcggca cccacacaga gcgaggcaga tgtgtgcctg cggcacgtgg
      661 cgctgcgggc gcggcagttg gatggcctga tggatggttt gctggcggtc acccggcaca
      721 cggcgctggc gagcctgttc aaagggcgct gttatcacgc cgtcacgtcg ggtgacggag
      781 gacaacgcga gcaggacacg tggcccggcg tgctgttaca accggttcac tacgccgcac
      841 ccgccgaccg gcagaagttc gacatcgtgc aatccatccg cacccgcacc ttgctgcggc
      901 aagcgcatcc gggcaaacgg ctcacggatg agtttcattt tcgccccccc ggcgaagtgc
      961 tggccgcgtg ccgtgagcat cgtaatctgc tcgaccacgc gcaggagatt gccgaccgct
     1021 gcacctttgt gctcagcccc gctccgcccc agtttcccgc gtttcatccc ccggacggtt
     1081 ccaccgctca cgcgttcctg cgcaagctcg tgttcgaggg ggtgcaccgc cgctaccggg
     1141 aacgcgccga ggggctgcgc ccgcaagtcg aggaagaatt ggccatcatc gccgaggtcg
     1201 gttacgagga atacttcctg gtcgtctggg acatgcttca agcgtgccgc cgacgcggca
     1261 ttgactggat cacccgcggc agcgcggcgg actcgctggt gtgctattgc cttggcatct
     1321 cggacgtgtg ccccctccgg ttcgacctgt atttccgccg cttcttgaac aaggagcgca
     1381 tggcgatgaa caagctgccg gacattgaca tggacttccc gcacgaccgg aaggacgacg
     1441 tggtggacct gctctttgaa aagtatggac gcgaacacac ggcggttgtg ggcgggtttt
     1501 ccacgtttca agcccgcagc gccgtggcgg acgtggccaa ggtgctgggc gtttcggaat
     1561 ttcaaatccg gcgcttcacc gagcacttcc cgtggacgag cgcccgcgcc atcaccgagg
     1621 cgttggcggg ccgtcaggag tgccgtgacc tgccgatcca ggaagaacct taccgcacgg
     1681 cactggcgat ggcggcgttc ctcgatggtt ttccgcgcta ccccaagatg cacccgtgcg
     1741 gggtggtgct ctcgcgccag ccgatgcccc aactcacacc cacgttcatg tcgagcaagg
     1801 gctacccgac cacgcacttc gacatggatg ccgtcgaggc catcggcttg gtcaaaatag
     1861 acattctcgc ccagggcggt ctggccgtta tgcgcgatgc gaagacttgg ctccggcagc
     1921 gcggcgtcga ggttgacctt ggccggctgg aaccgtggga agaccccgaa gtgtgggcaa
     1981 tgatcgccag cggcagcgcc cgcgctgtgc atcacatcga atctccggca atggtgggcc
     2041 tgtgccgcca gtgcaacgtg cggaacattg acgggctggt ggccattgtc agcgtcatcc
     2101 gtcccggcgc ggccaatgag ggcaagaagc aggcgttcgc ccggcgctat caaggacttg
     2161 aacccgtcac gtacccgcat ccgtcgctgg aaccgtgtct caagagcacg ttcggcctgg
     2221 tggtgtacga ggaacacatc ctgcaaatct gtgaagcctt tgccggtctg ccgcccggtc
     2281 gcgccgacgc gttgcgccgt gccctcggca agaacaaggc gccgctcatc gaggagatcc
     2341 gacaggagtt catcgccgcc gcccgcgcgc gcggacaccc ggacacgaag attgcggagg
     2401 tctgggactt ggtgggcggc ttcgccggct atgcgttctg caaggcgcac agcaccgcct
     2461 acggcgtcga ggcgtatcag agcgcgtggt tgaagcggta tcacccggtc gaattcatgg
     2521 cggcggtgct gtcgaacggc aagggattct atgacccgct ggtgtacgtg ctggaatgtc
     2581 accggctcgg catccccatt ctgccgccga ccgtgaatca gcccggcccg cagttcggcg
     2641 ttgaaagcca agccatccgc gtcccgctga cccgcgtgca ggggctgacg cagcgcacac
     2701 aggagcagat gactggggaa cggcagcggg gcgcgtttcg ttcgttgtca gatttctatc
     2761 ggcgcgtggt gcccagccgc gaggaaatgg aagccctgtt gcaggtgggc gcgtttgatg
     2821 agttcggcca atcgcgcacc gcgcagttct gggaagtcca acagctttac cacgcctttg
     2881 gcggcacctc tgactccagc caaggctggc tgttgcctgc gcccgagact aaccgcctgc
     2941 ctggcgtgcc tttgcaggag ccgacgcgcc gccagcggct gaaatgggaa acggaattgc
     3001 tcggcttcac cgccagcggg catccgttgg agctatatgg tgacatcgcg tgggacacct
     3061 actgcccggt gaaaaggctc ggtgagaacg tcggccaaca ggtcgtcacc tgcggcctca
     3121 ttatcgagca gcgcacccat catcaggtca cgggcgagcc tatgaaattc ctcacgctag
     3181 ccgactggac gggcatggtg gaaaccgaac tgttcgcgtc cacctaccgc agctatggac
     3241 tggcgacgat tcgctatcca gtgctggaag tggaggcgac cgtcgagcca tttgagaacg
     3301 ggcgcggttt ttcgttgcgt gtgttacggg caggcaggcc acgacaaaaa ctcagtgccg
     3361 tctcgaacgg gcgggaccga actcaagccg tgcgggcaag ctttcagcgg gaataaagtc
//
LOCUS       DATCGS010000014         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_298360, whole genome shotgun sequence.
ACCESSION   DATCGS010000014 DATCGS010000000
VERSION     DATCGS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298360"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..892
                     /locus_tag="VIL39_00205"
     CDS             149..892
                     /locus_tag="VIL39_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY5479877.1"
                     /translation="MGRLDQALVERGLCESREKAKRAVMAGLVKVNERLASKPSDSVK
                     PEDALTLVATDKFVSRGGLKLEHALNHFHLDVSGHTAIDLGASTGGFTDCLLQRGAAK
                     VYAVDVGQGQLAWKLRCDPRVVVMEKTNARALRPANFPPPFVSADLVVIDCSFISLRK
                     ILPPAVALLRPFAKIVALIKPQFEAGKAEADRGAGVIRDPVIHQRVLRELNEFVTTCG
                     GLRWLGVTESPLLGPAGNKEFLALIENSG"
     gene            876..1760
                     /locus_tag="VIL39_00210"
     CDS             876..1760
                     /locus_tag="VIL39_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5479878.1"
                     /translation="MKTLADRIKRVGLIGNSQKISGAGAVKRAADLIVAAGRKIYSDP
                     ATARLARLKVVVCADAARLARQVDLLVVFGGDGTMLRVAREIAGSRTPILGVNIGGLG
                     FLTAVPSANMPHALKCVWTGQFRFESRVLIQATGRCGGRLVDQTALNDIVIGRSVASR
                     LIELDVSVDGAPLTRYRCDGLIFSSPTGSTAYSLSAGGAVVFPTAEVLALTPICPHTL
                     SNRSLILPLTATIDVKIISPKPATILSSDGQVISELAAGDKITIRRARRTVRLMHLAG
                     SSFCETLRCKLHWRGASL"
     gene            1757..2794
                     /locus_tag="VIL39_00215"
     CDS             1757..2794
                     /locus_tag="VIL39_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5479879.1"
                     /translation="MTRLKDIAQRAGLAIMTVSKALRDKPDVSAATKARVKLLAQQMG
                     YVPDSTAQGLRMRATRLFGLVISSMTNPIYSRVVLAIEERAYELGYGVLLAHTLNKPE
                     REEACLRRLLGRRVEGLFIAPVYRMTAEAPIYQELIARGIPTVVLGHVAPFCSQFANV
                     ETDDLLASYAVTQHLLKLGHKRIAFLSGPVATPWTQERFEGYRRALREAGLDVDDRLV
                     FQAGQTIEDGAKAALQMINESSDATAVQAVNDVVAVGCAETLLSHGLRIPEDISVTGF
                     GNILLGAHFRVPLTTTRQPKFRLGSAAVDAMQALLRGQRPESKRLPAALMVRASTGTA
                     PATSRLKQPKL"
BASE COUNT          573 a         1028 c          924 g          608 t
ORIGIN      
        1 tggctgagga cgacatgtgc ctggccacgc ccgctctggc cagcgaccgc ctgctgatac
       61 ggagcgcggc ccggatttat tgcatcggcc ataagccgta agcggtttaa cctcgcggga
      121 tgagcggtgg ttgaagcata ctccggagat gggccgtttg gaccaggcgt tagttgagcg
      181 cggactgtgc gagagccggg agaaggcgaa gcgcgcggtc atggccggcc tggtcaaggt
      241 caacgaacgc cttgccagca aacccagcga ttcggtaaag ccggaagacg cccttactct
      301 ggtcgccacc gataaatttg tcagtcgcgg cggcctcaag ctcgaacacg ccctcaacca
      361 cttccacctc gacgtctctg gacacacggc aattgacctc ggtgcctcga ccgggggctt
      421 caccgactgc ctcttgcagc gcggcgcggc caaggtgtac gccgtggacg taggtcaggg
      481 ccagcttgcc tggaaactgc gctgcgatcc gcgtgtggtg gtgatggaaa agaccaatgc
      541 ccgcgctctt agaccggcaa acttcccgcc gcccttcgtt tcagcagacc tggtggtaat
      601 tgattgctcc ttcatttccc tgcggaaaat tctcccgccc gccgttgcat tgctccggcc
      661 atttgctaaa atcgtcgcat taatcaaacc gcagttcgaa gccggcaagg cggaagccga
      721 tcggggtgcg ggcgttattc gcgatccggt aattcaccag cgcgtgttgc gcgagttgaa
      781 cgagttcgtc accacttgcg gtggcctgcg ttggctgggc gtcaccgaat ccccgctact
      841 gggccccgcg ggcaataaag aattcctggc actgattgaa aactctggct gacagaatca
      901 aacgcgttgg actgattggg aactcccaga agatctccgg cgcaggggcg gtcaaacgag
      961 cggccgacct tatcgtggct gccggccgca agatctacag cgaccctgcc actgcccgcc
     1021 tggcccgctt aaaggttgtg gtttgcgccg acgcggccag gctcgcgcgc caggtggact
     1081 tgctggtggt gtttggcggg gatggcacca tgctccgtgt cgcccgcgaa atcgccggct
     1141 cgcgcactcc cattttgggc gtcaacatcg gcggcctcgg tttcctcacc gccgtcccat
     1201 ccgcaaatat gccgcatgcc ctgaaatgcg tttggactgg ccaattcaga tttgagtccc
     1261 gtgttctcat ccaggccacc ggccgctgcg gcgggcggct ggtggatcaa accgctctca
     1321 acgacatcgt catcggccgc agcgtggctt cccgcctcat cgaactggat gtcagcgtgg
     1381 atggggctcc acttacgcgc taccgctgcg acggcctgat cttcagctcc cccactggct
     1441 ccaccgccta ttccctttcc gccggcggcg cggtcgtatt cccaacggcc gaggtcttgg
     1501 ccctcacccc gatttgcccg cacacgctct cgaatcgctc cctcatcctg cctctgaccg
     1561 cgacgattga cgtcaagatc atcagcccca agcccgccac gatcttgagc tctgatggtc
     1621 aggtcatcag cgagttggcc gccggcgaca agatcacgat tcgccgcgcc cggcgaacgg
     1681 tacgcttgat gcacctggcg ggcagttcgt tctgtgaaac gttgcgctgc aagctccact
     1741 ggcgaggcgc ctctttatga cgcggcttaa agacatcgcc cagcgggccg gcctggccat
     1801 aatgactgtt tccaaggccc tgcgggacaa gccggatgtt tccgccgcca ccaaggcccg
     1861 ggtgaagctt ctggcgcagc aaatgggcta cgtccccgac tcgactgccc agggcttgcg
     1921 catgcgcgcc acgcgactct ttggcctggt catttcttcc atgaccaatc cgatttactc
     1981 ccgcgtcgtg ctggccatcg aggagcgggc ctacgagctg gggtatgggg tcttgctcgc
     2041 acacaccctg aataagccgg agcgcgaaga agcctgcctc cggcggctgc tcgggcggcg
     2101 cgtcgagggt ctgttcatcg cccccgtcta tcgcatgacc gctgaggcgc cgatttacca
     2161 ggagttgatc gcccgcggca ttcccaccgt cgtcctcggt catgtcgccc ccttttgcag
     2221 ccagttcgcg aacgtggaaa ccgacgacct cctggccagc tacgccgtca cccagcactt
     2281 gctcaagctg ggacacaagc gcatcgcctt tttgtcgggc cccgtcgcca cgccgtggac
     2341 ccaggaacgc tttgaagggt atcgccgggc cttgcgcgaa gccgggctgg acgtggacga
     2401 tcgcctggtc ttccaggccg gccagacgat tgaagacggg gccaaagcgg ccttacagat
     2461 gatcaacgaa tcctccgatg ccaccgccgt ccaggccgtc aacgatgtcg tcgccgtcgg
     2521 ctgtgcggag acgctgctga gccatggcct tagaatcccg gaggacatat cggtgaccgg
     2581 cttcgggaat atcttgctcg gggcacattt tcgcgtgccc ttgacgacta cccgccaacc
     2641 caagttccgg ctgggttccg cggcagtgga cgctatgcaa gcactcctgc gcggccaacg
     2701 ccccgaatcc aagcggctgc ccgccgcact catggtccgg gccagtaccg gtaccgctcc
     2761 tgccacatct cgcctcaaac agccaaagct gtaaggattc ttcctgggac ccaatggcgc
     2821 cgtcgtccgg aaacccatcg acgcggcaaa ccgttgcaat ctgaggcacg taacgccaaa
     2881 aacgctgcag aaagcgctat atgtggggtt cttcggaagc ggtcaaccac tacaggtagt
     2941 gtgccttggc tggtcccaat ttccaatccc agaagcgcgc ctcgcaggca gggctgctct
     3001 aaagtccggc cagttgagag aagagcatgt tctgcagccc tgaaatccga agaccgaagg
     3061 ccgaaacccg aattccgaga atggcctaat tcgattggct gccgctccgc cggggtggct
     3121 atcaattcgg acc
//
LOCUS       DATCGS010000015         4014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_100570, whole genome shotgun sequence.
ACCESSION   DATCGS010000015 DATCGS010000000
VERSION     DATCGS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100570"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1531
                     /locus_tag="VIL39_00220"
     CDS             194..1531
                     /locus_tag="VIL39_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5479880.1"
                     /translation="MNLDLSRYARLGVLAACVITVVSSALRAQAEQGSTNYLLSVSTE
                     RADAIYHKGEKVTFKIRLLLDQQPLNDAEVQWTISKDGVPPTRTGKLTLAEGGGTVSG
                     KLDEPGFLQCRVTFQTPAKTALNAVAGAGIDPLRIKPSLPVPVDFDAFWSAQKKKLAG
                     VPINARLTPVQSPRGEVECFDLQADCVGAPVSGYFARPVAAKARSLPIILTVQGAGVR
                     SSSLGGAAGWAKEGFLALDINAHGIPNGKPDGFYTELANGELKDYRAQGRESRDTVYF
                     RGMFLRLVRAIDFLIAQPEWDGRTLIVYGSSQGGAQSIVAAGLDSRVTFFAAGVPAMC
                     DHTGAAVGRISGWPKLVPTGAAGEPDPRVLEAARYYDAMNFASRTRAAGIVTVGFIDT
                     TCPPTSVYAAYNALKGKKEIFNDPTSTHKVSAGAGEAMRHAILRHVETMKKRK"
     gene            complement(1656..3857)
                     /locus_tag="VIL39_00225"
     CDS             complement(1656..3857)
                     /locus_tag="VIL39_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY5479881.1"
                     /translation="MNTRKLELEPKLELKAVVLAGGKEAAEGAAPMVLRKLGDRTILD
                     YVLANARQVVSADDLYAVVGDWQDPVRAQLGDKCHYVVQPQPLGTGHAVLQLFPTLKD
                     YQGNLLILYGDTPLFRPASIRGLLNRHALRQADLTLLTAILDKPLPYGRIIRDAGGRI
                     VDIIEEREASPVVRQIHELNVGAYIVSAPLIFSALEKLAPSKNGKYQLTDCVHQLIRS
                     GLRVESYQICDPDEIQGINTSKDLEQAEFILQKRLFRPRREEEQNQVSFGTGGWRAII
                     GEGFTLHNVRRLSQALANEITRRGDEKRGVLIGYDRRFLSNRAGEAAAEVFAGNNIPV
                     TLLTEDAPTPLITYATALQKAAYGLAFTASHNPPEWNGLKVFQGDGSLLLDDQTEQIE
                     TETNRLTLEEVIKIDLDIALERGLVHRSDFTNEYVDAVEALIDLEAIRRAGLKVIVDP
                     MYGVGQLTLGTILTEARCRVTFIHERHNPLFGGRSPAPNLEALRLLATHVVEDHYDLG
                     LATDGDADRIAIVDERGEYITINDILLLVYWYLHEIRGERGGVVRNLATTHLLDRLAT
                     KFGEQCIEVPVGFKHIASAMVAHNALLGGESSGGLTIRGHILGKDGIFASALIVEMLA
                     RTHEKISQLRRRVYDLTGRLYSLEDSIPSTPEMCVAVPRRLAQTPPDHIGPCKVVKVS
                     HVDGTKFYLDNDGWALVRFSGTEPILRLFVEAESPEVAAALIQALREFVMK"
BASE COUNT          761 a         1245 c         1249 g          759 t
ORIGIN      
        1 cgctttccct gacagcggta atccgctgat ccagcagaag tgccacccgc agccaaagcg
       61 gcgtcgcgct tcgcttgccg ccgcactcca catccgtggt gccgggacat cccgtcgact
      121 ttgtcaaccg ccctgcccat gccttgggag gtttctcctt gtcatctctg gggccaccga
      181 atgaaatcac tggatgaatc ttgatctttc gcgttatgcg cggctgggcg ttctcgccgc
      241 gtgcgttatc accgttgttt cttcggccct ccgcgctcag gcggagcagg gatccaccaa
      301 ctacctgctg agcgtgagca cggaacgagc tgacgcgatt taccacaagg gcgagaaggt
      361 gacgttcaag atcaggctgc tccttgacca gcagcccctg aacgacgccg aggtgcagtg
      421 gacaatctcc aaagacggtg tgccgccgac ccggacgggg aaattgacgc tggccgaggg
      481 cggtggaacg gtctcaggca aacttgacga gccgggattc ctgcagtgcc gggtaacctt
      541 ccaaacccca gccaaaaccg cactgaatgc ggtggccggc gcgggcattg acccgcttcg
      601 aatcaagccc agcctgcccg tgccggttga ctttgacgcg ttttggtcgg cgcagaagaa
      661 gaaactcgcc ggcgtgccga tcaacgcaag actcacgcct gtgcaatcgc ctcggggaga
      721 agtcgagtgc tttgacctcc aggccgactg cgttggggca cccgtgtcgg gctacttcgc
      781 caggccggtt gcagccaagg ccaggagctt gccgatcatc cttaccgttc agggggcggg
      841 cgtgaggagc tccagtttgg gcggggccgc cggctgggcc aaggaaggct tcctcgcgct
      901 cgacatcaac gcgcatggga ttccgaatgg caaaccagac gggttttaca cggagctggc
      961 taacggcgag ttgaaagact accgcgcgca ggggcgcgag tcgcgcgaca cggtctattt
     1021 ccgtggcatg tttctgcgcc tggtgcgagc gattgatttt ctcatcgcgc agcccgagtg
     1081 ggatggacgc acgctcatcg tttacggcag cagccagggc ggcgcgcaat ccatcgtcgc
     1141 ggcgggactc gactcgcgcg tgaccttctt cgccgccgga gtgcccgcca tgtgcgatca
     1201 caccggagcg gcggtcggcc gcatcagcgg gtggccaaag cttgtgccga ccggcgccgc
     1261 cggcgagccc gacccgaggg tgctcgaagc cgcgcgctat tatgacgcga tgaactttgc
     1321 cagccggacc cgggcggctg gcattgtgac cgttggtttc attgacacca cttgtccgcc
     1381 aacgagcgtt tatgcggctt acaacgcgct taaaggcaag aaggaaatct tcaatgaccc
     1441 gaccagcacg cacaaggttt cggcgggcgc aggcgaggcc atgcgccatg ccatcctccg
     1501 gcacgtcgag acgatgaaga aaaggaagtg aggggtggtg actcggtcaa gatagccagc
     1561 ccacgaatcc ggcatatccg agggggcgag cgcctattct cttagctcag tctcaacggc
     1621 gcgatcacgc ccaacggcgc catcgcgcct tcgactcact tcatcacaaa ttcccggagc
     1681 gcctgaatca gcgcggctgc cacctccgga gactccgcct cgacgaacag acgcaggatc
     1741 ggctcggtgc cggaaaagcg cacgagcgcc cagccatcgt tgtccaggta aaacttcgtc
     1801 ccatccacat gggagacttt gacgaccttg caagggccga tatgatccgg cggcgtttgg
     1861 gccaggcgcc gcggaaccgc gacgcacatc tcgggtgtgg aggggatgct gtcttccagg
     1921 ctatagaggc ggccagtgag gtcgtaaacc cgacgccgca actgcgagat cttctcatgc
     1981 gtgcgagcca gcatttcgac gatcaacgcc gaggcgaaga tgccgtcttt gcccagaatg
     2041 tgcccgcgaa tcgtcagccc gccgcttgat tctcccccca gcaaggcatt gtgggcgacc
     2101 atggcgctcg cgatatgctt gaagcctacc ggcacttcga tgcactgctc gccgaacttc
     2161 gtggccagcc ggtcgaggag atgcgtcgtg gcgaggttgc gcacgacgcc gccccgttcg
     2221 ccgcggattt cgtgcagata ccagtacacc agcagcagga tatcattgat cgtaatgtat
     2281 tccccgcgct cgtccacgat cgcaatccgg tcggcatcac cgtcggtggc caacccgagg
     2341 tcgtagtgat cttccaccac atgcgtggcc aaaagacgca gcgcctccag gttgggcgcc
     2401 ggcgagcggc cgccgaacaa tggattatgg cgctcgtgaa tgaacgtcac ccggcaccgc
     2461 gcctcggtga ggatggtgcc gagcgtgagc tgtccaactc cgtacatcgg atcaacgatg
     2521 accttgaggc cagcccggcg gatcgcttcg aggtcaatca gggcttccac cgcgtccacg
     2581 tattcgttcg tgaaatcgct ccgatggacg aggccgcgct ccagcgcgat atccaagtct
     2641 attttgatga cctcctcaag cgtgagccgg ttcgtctcgg tctcgatctg ctccgtctgg
     2701 tcgtccagca ggagggagcc atcgccctga aagactttga ggccgttcca ttcgggcgga
     2761 ttgtggctgg cggtaaacgc caggccatag gcggcctttt ggagcgctgt cgcgtatgtg
     2821 atgagcggtg tcggcgcgtc ttcggtcaac agcgtcaccg ggatgttgtt ccccgcaaac
     2881 acctcggcgg ccgcttcgcc ggcgcggttg gagagaaagc ggcggtcata gccgatgagg
     2941 acgccgcgct tttcatcgcc ccggcgcgtg atctcattgg cgagcgcctg cgacaggcgc
     3001 cggacattgt ggagcgtgaa accctcccca atgatcgccc gccagccccc ggtgccgaac
     3061 gaaacctggt tctgctcctc ctcgcggcgc ggccggaaga ggcgcttttg cagaatgaat
     3121 tcggcttgct ccaggtcctt gctcgtgttg atgccctgaa tttcgtccgg gtcgcagatc
     3181 tggtagcttt caacccggag ccctgaacgg atgagctggt gcacgcaatc tgtcaactgg
     3241 tacttgccgt tcttggaagg cgccagcttc tccagggcgg aaaagatcag cggcgctgaa
     3301 acgatgtacg cgcccacatt gagttcgtgg atttggcgca cgacgggcga cgcctcccgc
     3361 tcctcgataa tgtcaacgat gcgccccccg gcatcgcgaa tgatgcggcc gtaaggcaag
     3421 ggcttgtcca ggatcgcggt caacagcgtc aggtccgcct gccgcaacgc atggcggttc
     3481 agcaacccgc gaatagacgc gggccggaac aggggtgtgt cgccatacag gatcagcagg
     3541 ttgccctgat agtctttcag ggtggggaat aactgcagca ccgcgtgccc ggtgcccagc
     3601 ggctgcggct gaacaacata gtggcacttg tctcccagtt gggcgcgcac ggggtcttgc
     3661 caatcgccta cgacagcata gagatcgtcg gcggagacga cctgccgcgc attcgcgagg
     3721 acatagtcca ggatcgtgcg gtcgcccagc tttcggagca ccatcggcgc ggctccctcg
     3781 gccgcctctt ttccgcccgc gaggaccacg gccttcagct caagcttcgg ctctaattcc
     3841 agttttctcg tattcacact tgtcaacgat actcacctat ccagaggggt ggaaaaagac
     3901 aaaagttgta atgggtcagt gacggcggag acgacggtat cggagtgcgc ccgtcctcgg
     3961 gaaatgccgc taagatttgg aagccgaatt tgaagtgatt gtcgggtgac ggac
//
LOCUS       DATCGS010000016         3463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_496595, whole genome shotgun sequence.
ACCESSION   DATCGS010000016 DATCGS010000000
VERSION     DATCGS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496595"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..682)
                     /locus_tag="VIL39_00230"
     CDS             complement(221..682)
                     /locus_tag="VIL39_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5479882.1"
                     /translation="MRIPVTQMSNEALLRQLLDAIPSYVFLVDRDVTILDYNAAAGAF
                     LGVGQRSILRHRGGDVFHCLHSREVAAGCGHGPFCKSCSVRDAVNEAFAGKKCVRRQV
                     RMELCSGRKVKELHVLLTASPFAYQGHERVVLILEDLSGLSALQHLLPLSP"
     gene            complement(688..1821)
                     /locus_tag="VIL39_00235"
     CDS             complement(688..1821)
                     /locus_tag="VIL39_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5479883.1"
                     /translation="MKSPDHLLMVADSEHDANMLHAVGMFVPDPFIFLRTRGRDYVVM
                     SDLEIDRARKQAPHCCVLSLSRYQHKLRREGIKRPGFAQVIPALLRDRGIRRVVVPQD
                     FPYGLASDLQRSGIRVTARPGSFFPDRECKTAAEVKKLSAALLMAEIGMAEAIQVLHR
                     TKITKGRRLLYHEVPLTSEKLRAVIDTAILRAGGLASHTIVAGGRQGCDPHEEGYGPL
                     RANEPIILDIFPRSQKTGYFGDITRTVVRGLASEAVRKLYDTVLQGQKLAFRKMRPGV
                     PTAEVLTAVQEFFAREGYPTGRRNGRMQGFFHGTGHGLGLEVHETPRLGPTSAGKLKA
                     GQVVTVEPGLYYAEIGGVRIEDVALVTGTRPRNLTQFEKVLEV"
     gene            complement(1823..2446)
                     /gene="upp"
                     /locus_tag="VIL39_00240"
     CDS             complement(1823..2446)
                     /gene="upp"
                     /locus_tag="VIL39_00240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY5479884.1"
                     /translation="MKRVTVITHPLVQHNLTRLRDRRTGAQEFRRVLSEVAALMVYEA
                     TRSFTVVPVSVRTPLANTRGVRLRREVVLVPVLRAGLGMLDSILQLVPHARVGFIGLK
                     REETTLRATTYHKSLPPDLTPFEIILIDPMLATGGSAVAAMDLLVELGAKHVRMVNLV
                     AAPEGIRRVHARYPKLPIFTAAVDQALNDKGYILPGLGDAGDRLFGV"
     gene            complement(2449..>3463)
                     /gene="sppA"
                     /locus_tag="VIL39_00245"
     CDS             complement(2449..>3463)
                     /gene="sppA"
                     /locus_tag="VIL39_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY5479885.1"
                     /translation="LVLLVLLGISVLANLGHLLSGLVPMKVAGANSVGPRLEEVTSED
                     NGASSKIAVIEVNGIIMSRAVDQGGYNMVDVIKAQLKRATEDNKVKAVLLKVDSPGGE
                     VLASDEISRAIAEFQATRNGKPVVCSMGGLAASGGYYVSAPCRWIVANDLTITGSIGV
                     IMSSWNYRGLMDKVGVRPQTFKSGKYKDMLSGSRDPESITPEERQMVQSLIDETYGKF
                     KSVVAAGRKRAQEKNRDKGRALSEDWADYADGRILSGTEAFKLGFVDELGTFDDAVKR
                     AKSIAGIPRANLIEYQQRFDISDLFRLFGKSDSKAIKVDLGMEIPKLQAGQLYFLSPT
                     FLH"
BASE COUNT          650 a         1081 c         1071 g          661 t
ORIGIN      
        1 tttccttgac ccgtttgttc agctcgagga accgctcata ggcctggccc tcacgaggga
       61 tcggcttaat gcattgatga agttcctggg tcggctcggt ctggccgaat acggcggcag
      121 atgccaagac tagcgcggca acaaggcgag cgcgaacgga gacaaaagaa ctcatgcccg
      181 gaatgttggc gcgccaggct ggtttttcaa gtcgtttcag ttagggggaa aggggcagga
      241 ggtgctggag cgcggaaaga ccgctgaggt cctccaggat gagcaccacc cgctcatggc
      301 cctggtacgc aaagggagag gcggtgagaa ggacgtgcaa ctctttgacc tttcgtccgg
      361 aacacagttc catacggacc tggcgccgga cgcatttctt gccggcgaaa gcttcgttga
      421 ctgcgtcccg cacggaacaa ctcttgcaga aagggccgtg tccacacccg gcggcaacct
      481 ccctcgagtg caggcagtgg aacacgtcgc ccccgcgatg ccgaaggatg ctgcgttgcc
      541 caacgcccag gaaagccccg gccgccgcgt tgtaatccag gatggtcacg tcccggtcca
      601 ccaggaaaac gtaggacgga atggcatcga gcaactgccg cagcagggcc tcgttgctca
      661 tctgtgtcac tggaatgcgc atgggcttta aacctccagc actttctcga attgggtcag
      721 gttgcgcggg cgggttccgg tgacgagcgc gacatcctcg attcgcaccc cgccgatctc
      781 agcgtagtaa agccccggct ccaccgtgac cacttgaccg gccttcaatt taccagccga
      841 agtcggcccc aggcgcggcg tctcgtggac ttccaaaccc agcccgtggc cagtgccgtg
      901 gaaaaaaccc tgcatgcggc cgttgcgtct gccggtcggg taaccttccc gcgcgaagaa
      961 ctcttggacg gccgtaagca cttccgccgt gggcaccccg gggcgcatct tccggaacgc
     1021 cagtttctgg ccctgaagca ccgtgtcata cagcttccga accgcctcgc ttgccaggcc
     1081 gcggacgacg gtgcgggtga tgtcgccgaa atagccggtc ttctgcgagc ggggaaagat
     1141 gtccaggatg atgggttcgt tggcgcgcag cggaccatag ccttcctcat gggggtcgca
     1201 gccttgtctg ccgccggcga caatggtatg gctggcgagt ccgccggctc gaaggattgc
     1261 ggtgtcaatg accgcccgca gcttctccga cgtcagcggc acctcatggt atagcagccg
     1321 gcggcctttg gtgatcttcg tccggtgcag cacctggatg gcctcggcca tgccgatctc
     1381 ggccatgagc agcgcggcgc tgagtttctt cacctcagcg gcggtcttgc attcgcggtc
     1441 gggaaagaag ctgccggggc gagctgtcac cctgatgccg cttcgttgta aatcggatgc
     1501 aaggccgtaa ggaaaatcct gcggcaccac gacgcgccga atgccccggt cgcgcagcag
     1561 ggccggaatg acttgggcaa agccgggccg cttgatcccc tcacgacgga gtttgtgctg
     1621 gtagcggctg agcgagagga cgcaacaatg cggggcctgc tttcgggcgc ggtcgatttc
     1681 caggtcgctc ataaccacgt agtcccgccc acgggtgcgc agaaagatga aggggtccgg
     1741 cacgaacatg ccgaccgcgt gaagcatgtt ggcgtcatgc tcgctgtcgg ccaccatcaa
     1801 tagatggtca gggctcttca tctcaaaccc caaagagccg gtcgccggca tcgcccaggc
     1861 cggggagaat gtagccttta tcgttcaatg cctggtcaac ggcggcagta aagatcggca
     1921 gctttggata gcgagcatga acccggcgaa tgccttcggg cgcggcgacg aggttcacca
     1981 ttcgcacatg cttcgctccc agctccacca gcaaatccat ggcagcgacg gcgctgcctc
     2041 cggtggccag catgggatca atcaggatga tttcaaacgg ggtcaggtcc gggggcagac
     2101 tcttgtgata tgtggtggcc cgcaaggtag tttcctcccg tttcagccca atgaacccga
     2161 cgcgcgcatg aggaacgagt tgcagaatcg aatccagcat gcccaggccg gcgcgcaaga
     2221 ctggcacgag caccacctcg cgccggagtc gaacgccacg ggtattggcc aggggagtcc
     2281 tgaccgagac gggcaccacg gtgaacgagc gggtcgcctc gtaaaccatc agggcagcga
     2341 cctcgctgag cacccggcgg aactcctgcg cgccggtgcg ccggtcgcgc agccgggtga
     2401 gattatgctg caccaggggg tgcgtgatga cagtgaccct tttcaccctc agtgcaggaa
     2461 cgtgggtgaa aggaagtaga gctggccggc ctggagcttg gggatttcca tgcccaggtc
     2521 caccttgatg gccttgctat cgctcttgcc aaacaaccgg aacaggtcgg agatatcaaa
     2581 gcgttgctgg tactcgatga ggttggctcg tgggattccg gcgatgctct tcgcccgctt
     2641 gacggcgtcg tcgaaggtgc ccagctcatc cacaaacccc agcttgaagg cttcggtgcc
     2701 ggagaggatg cggccgtcgg cgtaatccgc ccagtcctcg ctcaaagcgc ggcctttgtc
     2761 cctgttcttt tcctgcgccc gcttgcggcc ggcggctacg acgctcttga acttcccgta
     2821 ggtctcgtca atcagtgact gcaccatctg ccgttcttcc ggcgtgatgc tttccggatc
     2881 gcgcgaaccg ctcaacatgt ctttgtactt cccgctctta aaagtctgcg gccgcacccc
     2941 gactttgtcc atcaggccgc ggtaattcca cgagctcatg atcacgccaa tgctgccggt
     3001 gatggtcagg tcattcgcca cgatccagcg gcagggcgcc gagacgtagt agccgccgga
     3061 cgcggccaag cctcccatcg agcacaccac cggtttgccg tttcttgtgg cttggaactc
     3121 ggcgatggcc cggctgatct catccgaggc caggacttcg ccgcccgggg aatccacttt
     3181 gaggagcacc gctttgacct tgttgtcctc cgtcgcgcgc ttcaactgcg ccttgatcac
     3241 atcaaccata ttatagccgc cctggtccac ggcccggctc atgatgatgc cattgacttc
     3301 gatgacggcg atcttgctgg acgcgccgtt gtcctcggag gtcacttcct ccagccgcgg
     3361 tcctacggaa ttagcgccgg cgactttcat gggaacaagg ccgctcagca ggtggcccag
     3421 gttggcgagc acgctgattc ccagcaacac cagcagcacg agg
//
LOCUS       DATCGS010000017         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_348203, whole genome shotgun sequence.
ACCESSION   DATCGS010000017 DATCGS010000000
VERSION     DATCGS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348203"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..652
                     /gene="rpsD"
                     /locus_tag="VIL39_00250"
     CDS             44..652
                     /gene="rpsD"
                     /locus_tag="VIL39_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5479886.1"
                     /translation="MARYTGPRVRISRRFGVPIFGPSKYLERRNYGPGMHGPKSRRKH
                     TDYGLGLIEKQKLRYYYGLLERQFRGVYERALRRRGVTGEHMLQILETRLDNVVYHLG
                     FANTRAAARQLVAHGHVKVNGRKVNIPSYGLKLNDIIEIKNSNAARQLATKNLEVAGS
                     RIVPDWLSLNKEALKGVVMRVPTRDEIQPIANEQAVVEFYSR"
     gene            814..1839
                     /locus_tag="VIL39_00255"
     CDS             814..1839
                     /locus_tag="VIL39_00255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY5479887.1"
                     /translation="MPVRLGRFEMPKRLTKEDSTATDTYAKFVAEPFETGYGYTIGNS
                     LRRVLLSSLEGAAVCSIKIEGAMHEFATVDGVVEDVTDIVLNLKKILFKAHSRDPQML
                     LLSVNKEGDVTAADIQLNQNIELVNPTQHICTLDKKKKFEMELEVKVGRGYLPGDQNK
                     KPNQPIGVVAIDSLFSPVTRVRYAVESARVGQRTDYDRLILEIYTDGRITPDDALTQA
                     SAILQHHLDVFVGYDKNAVEFEEVVDKQDEEKTKLKKLLNMSVNEIELSVRAANCLNN
                     ANITTVGQLAMKSEQEMLKYRNFGKKSLNEIKDKLSGLGLTLGMTFEAELLEATKEQT
                     PAAPATH"
     gene            1916..2377
                     /gene="rplQ"
                     /locus_tag="VIL39_00260"
     CDS             1916..2377
                     /gene="rplQ"
                     /locus_tag="VIL39_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY5479888.1"
                     /translation="MRHLKRTAKLGRTGTHRNAMLANMVCSLIKHKRITTSLAKAKAA
                     RSVAEKMVTLGKSGTLHDRRLAAARLHQEDAVKILFNEIAPTQKDRRGGYTRIIRMNQ
                     RQGDACQKAILEWVDLGVVAATPAEEKPAKETKPRDAKETKPEEAKAEASK"
     gene            complement(2452..4164)
                     /locus_tag="VIL39_00265"
     CDS             complement(2452..4164)
                     /locus_tag="VIL39_00265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5479889.1"
                     /translation="MQASSDTKGSAQRLAALAAPPDAAAESITLYHWLVVLFASCGWL
                     FDCMGQRIFVLSREPALRELLGATASDDKVKYWGGIATLLMMVGWATGGILFGMLSDH
                     YGRVKAMVSTLLAYTIFSGLSGFSRTGVEFLVFRFLFGLGVGGMFGAATTLVAESVPK
                     HFRTLALGSMQALSAFGTMLASWLSLTITPGVEGFWGHYSGWQVLSFVSVFPAILAVP
                     MALILKEPQAWIKAKAEAATGGADKAVGSFADLFRHPRWRRSTLVGICLGVAGMVGLW
                     GIAFFSPELITTAFKNRPLQVEEILKPTQLCAALKSAANPGIAHLKAKLSPDVIQQLE
                     QVGSGKEVPSATVQALVQDLNRLIQQGDLHDEEAFQSVNLKKRTKNLIQLVQKNGERQ
                     NIISLNRQLVEQLFPGTIRELQKTIDRTRSRGTLLQDVGALLGMFAFTFAAAYFSRRT
                     AFLGSFVLCLCSVSFVFYSLKTETDVLWMLPLMGFATLSCFAGYSIYFPEIFPTRLRG
                     TGVGFCYNTVRYLAAPFPFLLGWLSTLMPFRTVAIVMSSIYLVGIIALIWAPETKGQP
                     LPED"
     gene            complement(4401..>5473)
                     /locus_tag="VIL39_00270"
     CDS             complement(4401..>5473)
                     /locus_tag="VIL39_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5479890.1"
                     /translation="HFHTVLERLMATSLRVLLIATRIEDLPRHVTHLLCVAQCRVVAA
                     GPRAQVLSLPRVKQLFSTPKTSLGRRADIPVRSNTRTLHISSTFETVPPAPPFCGQEC
                     PRAVNVGNNPPDWHPKSPVSELVQLRNVSVRYGDTIILSDINWTVRAGESWALLGPNG
                     SGKTTLLSLILGDNPQVYTNDVVVFGRPRGSGESVWEIKQHIGWVSPELHLHFNDSAT
                     CFEVVASGFHDTVGLFQSASARQRAVARQWLARFQLLEFAQRPLFSISAGLQRMVLLA
                     RAVVKNPRLLILDEPCQGLDSAHRALFVRAVDALIRAGTVTVIYVTHRPDEIPPSITR
                     VLRLPQSRQFRNSHLSSTCGTT"
BASE COUNT         1204 a         1594 c         1695 g          980 t
ORIGIN      
        1 gtctaaccct caactgaaaa agatcaacta cccactttag aaaatggctc gttatactgg
       61 tccccgcgtt cgcatcagcc gccgctttgg cgtccctatc tttggcccgt cgaagtatct
      121 tgaacggcgc aattacggcc ccggcatgca tggcccgaag tcgcgccgca agcacaccga
      181 ttacggtctg ggtctgattg agaaacagaa actccgctat tactacggtt tgctcgagcg
      241 ccaattccgg ggcgtttacg agcgcgctct aaggcgccgc ggagtcaccg gtgagcacat
      301 gctgcagatc ctggaaaccc ggctggacaa tgtcgtttat catcttggct tcgccaatac
      361 ccgggcggct gcccgacagt tggtcgccca cggccatgtg aaggtcaatg gccggaaggt
      421 caacattcct tcctatggcc tcaagctgaa tgacatcatc gagatcaaga actccaatgc
      481 cgcccgccag ttggccacca agaatctgga agtcgccggc agccgtatcg tgcccgattg
      541 gctctcgttg aacaaagagg ccctcaaggg cgtcgtcatg cgcgttccaa cgcgtgacga
      601 gatccagccg attgccaacg agcaggctgt cgtcgaattt tattcccgct aaaccaatga
      661 aacaggattg ttggactacg ggattgacgg aaggccgcac tgcgctcagt ctgtcatttc
      721 acgaatccag caattcatag caattatacg ggcctctcca gccggcggga atactggccg
      781 gaggggtcag attaaaattg ttgaaaggaa catatgccag tacgtttagg acgtttcgaa
      841 atgcccaagc ggttgacgaa agaagactca accgccaccg atacctacgc caagtttgtt
      901 gctgagccgt ttgaaaccgg ctacggctat accattggca actctctccg gcgagtgctg
      961 ctttcctccc tggaaggcgc cgccgtctgc tccatcaaga ttgaaggggc gatgcacgag
     1021 ttcgcgaccg ttgacggcgt cgtggaagat gtcacggaca tcgtcttgaa tctcaagaag
     1081 attctgttca aagcgcactc gcgcgatccc cagatgctgt tgctctccgt taacaaagag
     1141 ggcgatgtca cggccgctga catccagctc aaccagaaca tcgagctggt caatcccacg
     1201 cagcacatct gcacgctcga taagaagaag aagtttgaga tggaattgga ggtcaaggtg
     1261 ggccgcggct acctgccggg cgaccagaac aagaagccga atcagccgat cggtgtcgtt
     1321 gccattgact ctctcttttc gccggtgacc cgcgtccgct acgctgtcga aagcgcccgc
     1381 gtcggccagc gcaccgatta cgaccgcctc attctggaga tttacaccga cggccgcatc
     1441 acgcccgatg atgccctgac ccaggcctcc gctatcctgc agcaccacct cgacgtgttt
     1501 gtcggctacg acaagaatgc cgttgagttt gaggaggttg tggacaagca ggatgaagag
     1561 aagaccaagc tcaagaagct gctcaacatg agtgtcaacg agatcgagct gagcgtccgt
     1621 gcggccaact gcttgaacaa cgcgaacatc accaccgtcg gccagctcgc gatgaagtcc
     1681 gagcaggaga tgctcaagta tcgcaacttc ggcaagaagt cgctcaatga aatcaaagac
     1741 aagctgtccg ggcttggttt gacgctgggc atgaccttcg aggcggagtt gctggaggcc
     1801 acgaaagaac agactccggc cgcgccggct acccattagc aggatagtat tgcgttgagg
     1861 gggcggcagc ggcttgccct tggacaccaa aaacaccgat taccagtcac gatttatgcg
     1921 acaccttaag cgaactgcca agttgggccg caccggcaca caccggaacg cgatgctcgc
     1981 caacatggtt tgcagcctga tcaagcacaa acgcatcacc accagcctgg ccaaggccaa
     2041 agccgctcga tctgtggcgg agaagatggt cacgctcggc aagagcggca ccctgcacga
     2101 ccgccgtctg gccgccgcgc gcctccacca ggaagatgcg gtgaaaatcc tcttcaatga
     2161 gatcgcgccc acgcaaaaag accgccgggg cggctacacc cgtatcatcc ggatgaacca
     2221 gcgccaggga gacgcctgcc agaaggccat cctcgaatgg gtggatcttg gcgtggtcgc
     2281 cgcgaccccg gctgaagaga agccggccaa ggaaaccaag ccgagggacg ccaaggaaac
     2341 caagccggag gaagccaagg cagaggccag caagtaggcg gaccctgaac ctctatttca
     2401 acgcccgtcc cttttgagga cgggcttttt tttctaccgg gcgaccggcc ttcaatcttc
     2461 cggcaagggc tggcccttgg tttccggggc ccaaatcagg gcgatgatgc cgacgaggta
     2521 gatggaggac atgacgatgg ccacggtgcg gaagggcatc agcgtgctca gccagccgag
     2581 caggaacggg aagggcgccg ccaggtagcg gacggtgttg tagcagaagc ccacgccggt
     2641 gccgcgcagg cgcgtgggaa agatttccgg gaagtagatc gagtagcccg caaaacacga
     2701 cagcgtggca aagcccatca gcggcagcat ccagagcacg tctgtctccg ttttcaggga
     2761 atagaacaca aacgacaccg agcagaggca gaggacaaat gatccaagga atgccgtgcg
     2821 ccggctgaaa tacgcggccg cgaaggtgaa cgcgaacatt cccagcagcg cgcctacgtc
     2881 ctggaggagc gtgccccggc tgcgggtcct gtcgatggtc ttttgtaact cgcggatagt
     2941 gcccgggaag agctgttcca ccagttgccg gttgagcgaa atgatgttct gccgttcgcc
     3001 gttcttctgt accagttgga tcaggttctt cgtgcgcttc ttgaggttca cggattgaaa
     3061 ggcttcctcg tcgtggaggt cgccttgctg gatcaggcgg ttgaggtcct gcacgagcgc
     3121 ctggacggtg gccgacggaa cttccttgcc gctccccacc tgctccaatt gctggatgac
     3181 gtcgggcgac agtttggctt taaggtgggc gatgcctgga ttggccgcgg atttcaaggc
     3241 ggcgcagagc tgtgtgggct tgaggatttc ctcgacctgc aagggccggt tcttgaaggc
     3301 ggtggtaatc aactccggcg agaagaacgc gattccccac aagcccacca tgccggctac
     3361 acccaggcag atgccaacca gcgtgctgcg gcgccagcgc ggatggcgaa acaggtccgc
     3421 aaacgagccg acggccttgt cggcgcctcc ggtggctgct tccgccttgg cctttatcca
     3481 ggcctggggt tccttcaaga tcagcgccat cggcaccgcg aggatggcgg gaaaaacgct
     3541 cacaaaggac agcacttgcc agccgctgta gtgaccccag aagccttcca cgccgggggt
     3601 gattgtgagg ctgagccagg aggccagcat ggtgccgaac gctgacagcg cctgcatgga
     3661 accgagcgcc agcgtacgga aatgcttcgg cacactctcc gccaccaggg tcgtcgccgc
     3721 cccgaacatc ccgcccacgc ccaagccaaa caggaaacgg aagaccagga actcgacgcc
     3781 cgtgcgcgaa aaaccggaca ggccggagaa gatcgtgtag gcaagcaggg tgctgaccat
     3841 ggccttcacc cggccatagt ggtcgctcaa catgccgaag aggatgccgc cagtggccca
     3901 gccgaccatc atgagcaacg tggcaatgcc gccccaatac ttcactttgt cgtccgaagc
     3961 ggtggccccc agcaattccc tcagggccgg ttcccgcgac agaacaaaaa tccgctgtcc
     4021 catgcagtca aacaaccacc cgcacgaggc gaagaggacg acgagccagt gatagagggt
     4081 gatggattca gcagcggcgt ccggtggcgc ggcgagggca gccagccgtt gggctgagcc
     4141 cttggtgtcc gacgatgctt gcattggcgt ccatactccg aaatagcgtc ggcgtatgtc
     4201 aagttgcggt tgccaattcg cgcggcgcca attcgcgagg ccatacagag cgtcataatt
     4261 gcttgcgcca cgtgagggca cgtggctgcg aatgatagtc ggtcccgtaa aacaggtccg
     4321 cgacgcagtc ctcttttgaa accacaccaa aatagccgcc catatcaagt gctcgaagtt
     4381 agaaattcaa cggacgcagg ctaggttgtg ccgcacgtcg aactcaagtg tgaattgcgg
     4441 aattgccggg attgcgggag gcgcaagacg cgggtgatgg atggggggat ctcgtcaggg
     4501 cggtgcgtga cgtagattac cgttaccgtc ccggcgcgga tgagagcatc cacggcgcgg
     4561 acgaagaggg cccggtgcgc cgaatcaagg ccctgacagg gctcgtccaa aatcagcagg
     4621 cggggattct tgaccactgc ccgggccagg agaaccatcc gctggaggcc cgccgaaata
     4681 gagaacaagg gccgttgcgc gaattcgagt aattggaacc gggccagcca ttgccgcgcc
     4741 acggctcgct gacgggctga cgccgactgg aagagtccaa cggtgtcgtg gaagccggag
     4801 gccacgactt cgaagcaggt cgccgaatcg ttgaagtgga ggtgcagctc gggagacacc
     4861 cagccaatgt gctgcttgat ctcccagacg gattcgccgc tgcctcgcgg ccggccgaag
     4921 acgacgacgt cgttggtata gacctgtggg ttgtcgccga ggatgaggct gagcagagtg
     4981 gtcttgcccg acccgttggg tcccaggagt gcccagcttt ctccggcgcg gacggtccag
     5041 ttgatatcgc tcaggatgat ggtgtcgccg tagcgcacgc tgacgttgcg gagctggacc
     5101 agctcggaga caggcgattt tggatgccag tccggaggat tgtttccgac gttgacggcg
     5161 cgcggacatt cttgtccgca gaacggtgga gcgggaggaa cggtctcgaa ggtgcttgag
     5221 atgtggagcg ttcgcgtgtt gctgcggaca ggaatgtccg cgcgccggcc gaggctcgtt
     5281 ttgggggtcg agaagagctg cttcaccctc ggcagggaca gtacctgcgc gcgcggcccg
     5341 gcggccacga cccggcattg agcgacacag agcagatgcg tgacgtggcg gggcaggtct
     5401 tcgatccggg tcgcaatgag cagcacgcgc agggatgtgg ccatcaggcg ttcgaggaca
     5461 gtgtggaagt gcg
//
LOCUS       DATCGS010000018         3586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_595695, whole genome shotgun sequence.
ACCESSION   DATCGS010000018 DATCGS010000000
VERSION     DATCGS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595695"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VIL39_00275"
     CDS             complement(<1..1128)
                     /locus_tag="VIL39_00275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5479891.1"
                     /translation="MSLTFQCRSCGSAHGDLVLDLGVQPLANNLLRPEDLAKPEPKFP
                     LRLAVCRACWLLQITDLVPPVDLFSEYLYFSSFSDLMLRHAREAAQRYTSEFQLGPGS
                     LVVEIASNDGYLLKNFVAAGVPCLGIEPAANIAKVAREQGVETLVEFFGQGLAGRLAG
                     AGRQADLILGNNVFAHAPDTNDFVAGLRALLKPQGRVVFEFPYAADFIEKTEFDTIYH
                     EHVFYFSLTALKPLFERHGLTVFHVERLPIHGGSLRLFAGVTGAHAVQPSVTALLAEE
                     QRKGLAGLSYYQSFAARVRELKTALNTRIAELRRAGHTIAAYGASAKGSTLLNFYGLG
                     PAQLDFVVDRSTYKQGRLTPGTHLPILPPEELLARQPGYTLL"
     gene            complement(1125..2264)
                     /gene="rfbG"
                     /locus_tag="VIL39_00280"
     CDS             complement(1125..2264)
                     /gene="rfbG"
                     /locus_tag="VIL39_00280"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5479892.1"
                     /translation="MEQRQGALEGLVMFLNYYQGKRVLVTGHTGFKGAWLSLWLKQLG
                     ARVIGLSLPAPTQPNLHELVGAHACEREIICDIRDAGALQAAVAGAQPDVILHLAAQA
                     LVRRSYAAPLETLVTNAIGTANLLEAVRLTGLGCPVLIVTTDKCYENREWDFAYRETD
                     PLGGHDVYSASKAAAEIIAHSWRRSFFAPDPRLGPVATARGGNVIGGGDYAAERIVPD
                     CVRALLASQALIVRNPQAVRPWQHALECLSGYLWLAARLGTEGKQSPLNSAFNFGPDP
                     AARQPVRRLVEEFLRHWPGSWVDASDPKAPHEAALLTLSIDKAAARLAWRPVWTFAEA
                     IQHTAVWYHRRHVARADDLLQFSISQIETYTESARRQGVAWAQAS"
     gene            complement(2228..3001)
                     /gene="rfbF"
                     /locus_tag="VIL39_00285"
     CDS             complement(2228..3001)
                     /gene="rfbF"
                     /locus_tag="VIL39_00285"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY5479893.1"
                     /translation="MKVVILCGGLGTRLREETEYRPKPMVPVGERPILWHIMKTYAHH
                     GHKDFILCLGYKGEAIKEYFRNYHWNTSDITLRLGPNPQIKYHNRHDEEDWTVTLLDT
                     GQATMTGGRLKRVLPFIEEDTFLMTYGDGLTSSDITASIACHRKHGRIATITAVQPPG
                     RFGDLEIKDGLVTSFKEKAEHQVAYINGGYFVLNRRIGAYLTDDTCVFEQAPLGRLAD
                     EGQIAAYPHTGFWQCMDTYREEQLLTGLWNSGKAPWKVW"
     gene            complement(3107..>3586)
                     /locus_tag="VIL39_00290"
     CDS             complement(3107..>3586)
                     /locus_tag="VIL39_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479894.1"
                     /translation="GDYRLALNPVPAQAFATAVVSYGFVVHATVTSGGSGYVTSPAVS
                     IVGGGGSNATAVSHISGGVVTGITISDAGIGYTNAPTVRIAPPPAAAMFPTVEPVMRV
                     DSANLAPYNNYQIQLKPDLGGTWGNWNDGLFSPTDMTNSQYLFITNGAGFFRLQYVP"
BASE COUNT          638 a         1080 c         1244 g          624 t
ORIGIN      
        1 cagcaaggtg tagccgggct ggcgggccag cagttcttcc ggcggcagaa tcggcaggtg
       61 cgtgccgggc gtgaggcggc cttgcttgta ggtgctgcgg tccacgacaa agtccagttg
      121 cgccgggcca aggccgtaga aattcagcag cgtgctgcct ttggcggaag cgccgtaagc
      181 cgcaattgtg tgaccggcgc ggcgcaactc ggcgatcctg gtattcaggg cggtcttcag
      241 ctcccgcacg cgcgcagcga agctctggta gtaggacagg ccagcgagac ccttgcgctg
      301 ctcctcggcg agcaacgctg tcacgctcgg ctgaacggcg tgggcgcccg tcacgcccgc
      361 gaagagccgc agcgagccgc cgtgaatcgg gaggcgctca acatggaaga cagtcaaacc
      421 gtgccgctcg aacagcggct tgagggccgt caggctgaag tagaagacgt gctcgtggta
      481 gatcgtgtcg aattcggtct tctcgatgaa gtccgcggcg taggggaact cgaacacgac
      541 gcggccttgc ggcttcagca aggcgcggag gccggcgacg aagtcattcg tatccggcgc
      601 gtgggcaaag acattgttgc cgaggataag gtcggcctgc cgcccggcgc cggcgagacg
      661 gcccgcgagg ccctggccga agaactcgac cagcgtctcg acgccctgct cgcgggcgac
      721 cttggcgatg ttcgccgccg gttcaatgcc caggcagggc acgcctgcgg ccacgaagtt
      781 cttcaggagg taaccgtcgt tgctggcgat ctcgacgacc aggctgcccg ggccgagctg
      841 gaactccgaa gtgtaacgct gcgctgcttc gcgggcgtgg cgcagcatca ggtcggagaa
      901 cgaggagaaa tacagatact cggagaagag atccaccggc ggcacgaggt cggtgatctg
      961 caagagccag cacgcgcggc agacggcgag gcgcagcggg aacttcggct ccggtttggc
     1021 gaggtcttcg gggcgcagga ggttgttcgc cagcggttgc acgccgaggt cgaggacgag
     1081 atcgccatgc gcgctgccgc aggaacggca ttgaaaggtc aggctcatga tgcttgcgcc
     1141 caagcgactc cctgtcggcg ggcggattcg gtgtaggtct cgatttggga gatggagaac
     1201 tggagcaggt cgtccgctct ggcgacgtgg cgccggtgat accagacagc ggtgtgctgg
     1261 atggcctcgg cgaaagtcca gaccggccgc caggcgagcc gcgcggccgc cttgtcgatt
     1321 gaaagcgtga gcagcgcggc ctcatgcggg gccttcgggt cggaggcatc cacccacgag
     1381 ccgggccagt ggcgaagaaa ttcttccacc aaccggcgca cgggctgccg cgccgccggg
     1441 tcggggccga aattgaaagc ggagttcagc ggcgattgct tgccttcggt gcccagccgc
     1501 gcggcgagcc agaggtaacc gctcaggcac tcgagcgcat gctgccaggg ccgtaccgcc
     1561 tgggggttgc ggacgatcag ggcctggctt gccagcaggg cccgcacgca atccggcacg
     1621 atgcgctccg ccgcgtaatc gcccccgcca atgacattcc cgccgcgggc cgtcgccacc
     1681 gggccgagcc gcgggtcggg cgcaaagaac gagcgccgcc aggagtgggc gataatctcc
     1741 gccgccgcct tgctggccga atagacatca tgcccgccga gcgggtcggt ctcgcgatag
     1801 gcgaagtccc actcgcgatt ctcgtagcac ttgtcggtgg tgacgatgag caccgggcag
     1861 cccaagccgg tcaatcgcac cgcttccagc aaattggcgg tgccgatggc gttggtgacc
     1921 agcgtctcca gcggcgcggc gtaggagcgg cggaccagcg cctgcgcggc gagatgcaga
     1981 atcacgtccg gctgcgcgcc agcgacggcg gcctgcagcg ccccggcgtc gcggatgtcg
     2041 cagatgattt cccgctcgca ggcgtgcgcg ccgacgagtt catgcaggtt cggctgggtg
     2101 ggcgccggca gactgagacc gatgacgcgc gcgccgagct gcttgagcca gagggaaagc
     2161 cacgcgcctt tgaagcccgt gtggccggtg acgaggacgc gcttgccctg gtagtaattg
     2221 aggaacatca ccagaccttc cagggcgcct tgccgctgtt ccacaggccg gtgagcaact
     2281 gctcttcgcg gtaggtgtcc atgcactgcc agaagccggt gtgcgggtag gccgcgatct
     2341 ggccctcgtc ggcgaggcgg cccagcgggg cctgttcgaa gacgcaggtg tcgtcggtca
     2401 ggtaagcgcc gatccggcgg ttgagcacga aatagccgcc gttgatgtaa gccacctggt
     2461 gctcggcctt ctccttgaag gaggtcacca ggccatcctt gatttccagg tcgccaaaac
     2521 ggcccggcgg ttggacggcg gtgatcgtgg cgatcctgcc gtgcttccgg tggcaggcga
     2581 tggaagcggt gatgtcgctg ctggtcaagc cgtcgccgta ggtcatcagg aacgtatcct
     2641 cttcgatgaa cgggagcaca cgcttgagcc ggccgccggt catggtcgcc tggcccgtgt
     2701 caagcagcgt gaccgtccaa tcctcctcgt cgtgccggtt gtggtatttg atctgggggt
     2761 tgggcccgag acgaagggtg atgtcgctcg tgttccaatg gtaattgcgg aaatactcct
     2821 tgatcgcctc gcccttgtag cccaggcaca ggatgaagtc cttgtgccca tggtgggcgt
     2881 aggtcttcat gatatgccag aggatggggc gctcgcccac cggcaccatg ggtttgggcc
     2941 ggtattccgt ttcctcgcgc agccgtgtgc ccagaccgcc gcatagaatg acgactttca
     3001 ttgtttgctt tgtactgaag cgccgaaggg agctggtcca tttcgggccg cgcaggttat
     3061 agcctttcag gcgggcgaac gcaatggcga ttcgcgggcg cactctttac ggcacatatt
     3121 gcagccggaa aaaaccggcg ccattcgtaa tgaaaaggta ttgcgaattg gtcatgtcag
     3181 tcgggctaaa taatccgtca ttccaattcc cccacgttcc gccgaggtcc ggcttgagtt
     3241 ggatctgata attgttatac ggagccagat tggcggaatc gacccgcatt acaggctcta
     3301 ccgttgggaa catagcggcg gcaggaggcg gcgctatgcg aaccgttggc gcgttggtgt
     3361 agccgatgcc agcatcggag atggtgatgc ccgtcaccac gccaccagag atgtgagaaa
     3421 ccgccgtggc attgctgccg ccgccgccaa cgatggagac tgccggactg gtgacatagc
     3481 cggaaccgcc gctggtcacc gtcgcgtgga cgacaaaccc atagctcact actgctgtgg
     3541 cgaatgcttg agccgggacc ggattgagcg caagccggta atctcc
//
LOCUS       DATCGS010000019         4362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_150319, whole genome shotgun sequence.
ACCESSION   DATCGS010000019 DATCGS010000000
VERSION     DATCGS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150319"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VIL39_00295"
     CDS             complement(<1..609)
                     /locus_tag="VIL39_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY5479895.1"
                     /translation="MISRQDLRRVGDYLYEIPQTFRHDMRVSAHFYADDQILSKALED
                     RSLEQLVNTTTLPGVMPPAMAMPDIHQGYGFPIGGVVATRLPGGVISPGGVGYDINCG
                     VRLLAAHITADEVRPYLAELANALYERLPSGVGVKGHLHVNKSELDRILAEGSGWALR
                     EGYARSDDVEHTEERGHMAGADPERVSKGAKERGTDQVGTLGS"
     gene            complement(695..1108)
                     /gene="erpA"
                     /locus_tag="VIL39_00300"
     CDS             complement(695..1108)
                     /gene="erpA"
                     /locus_tag="VIL39_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY5479896.1"
                     /translation="MTVTTVEEAASEVQAPVITLTQSAAEKLQGLMQEKGLGDAYSLR
                     VFVGGGGCSGFQYGMTFDNNPRDVDIVFAEHGLRVIVDPRSLPYIVGANIDYEDSLMG
                     GGFHIENPNAVSSCGCGNSFRTKDDAGGGGCESSH"
     gene            complement(1129..2142)
                     /gene="moaA"
                     /locus_tag="VIL39_00305"
     CDS             complement(1129..2142)
                     /gene="moaA"
                     /locus_tag="VIL39_00305"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5479897.1"
                     /translation="MSFITLVDRPGAMYDSYGRHLHYLRVSLTDACNLRCVYCMPEDM
                     VFRKQASLLQYEELLLLVRVAASLGVDKIRLTGGEPTVRRGVVDLVRGIKSVPGIRRL
                     TMTTNGVRLAELAAPLAEAGLDQVNISIDSLDPARFRQITRRGDLEDVWQGLIAAEAA
                     GLEPIKINCVVTRGFNDGDVVDLARLTFEHPWDVRFIELMPFGKVSDFQHSQVVPSTE
                     TRARIEVAFGPLIAMPGWDGHDPARPYGLPGAQGRLGFISSVSEPFCEGCTRLRLTAD
                     GKLRLCLLRDDEADLLTPLRAGADFATLRQLMADAAYHKPWGHRLETEEIPLSREMSQ
                     IGG"
     gene            complement(2159..2800)
                     /locus_tag="VIL39_00310"
     CDS             complement(2159..2800)
                     /locus_tag="VIL39_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEY5479898.1"
                     /translation="MQTVRNHIVEILKENGTATVAELAGKLGMAQVSVRHHLDILVGE
                     DLVESAGVRRRNGAGRPSQVYSLTQQASKLFPQRHQVLANEMLSAMKRLLPAKELEGV
                     LLRLADKTVQDAPLARPDQAVEDRLNQVAEFLSGKGYTARWEVTDGYYRIFTCNCPYS
                     GVSEQNPELCEMDRTMMQRLVPEGVRRESRIVDGSPRCAYVLLLQPVAHSEKA"
     gene            3255..3464
                     /locus_tag="VIL39_00315"
     CDS             3255..3464
                     /locus_tag="VIL39_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479899.1"
                     /translation="MATITGAFPVLFLIARPAAGKSEIIDYLKRAVFENEDDVTTTRG
                     KALGDRLQETMGPLWTFFEAFHNNP"
     gene            4012..>4362
                     /locus_tag="VIL39_00320"
     CDS             4012..>4362
                     /locus_tag="VIL39_00320"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEY5479900.1"
                     /translation="MNNVYVADMPGALPPKEAWEVARSQLRLQMSKSTYETWVRDSVY
                     MAHEDGAFVIGVPNAYAKDWLSMRLRPLIKRTLSSIVGHSVDVTFVVRSVPLAESLKP
                     AGGLLLDMDKSRQAD"
BASE COUNT          895 a         1368 c         1247 g          852 t
ORIGIN      
        1 cgagccaagc gtccccacct ggtccgtccc gcgttccttg gcgcccttgc tgaccctctc
       61 cgggtcggcc ccggccatgt ggccgcgctc ctcggtatgc tccacgtcgt cgcttcgcgc
      121 atagccttca cgcagcgccc atccgctgcc ctcggccagg atgcggtcca gttcggactt
      181 attgacgtgc aagtgccctt tgacgcccac cccactgggc aagcgttcgt agagcgcgtt
      241 cgccagctcc gccaggtaag ggcgcacctc gtctgccgtg atgtgcgccg ccagcaacct
      301 gacaccacaa ttgatatcgt acccaacccc accgggcgag ataactccac cgggcaaacg
      361 cgtcgccacc acaccgccga tggggaaacc gtaaccctga tgaatgtccg gcatggccat
      421 tgccggcggc attaccccag gcagagtagt ggtattgact aactgttcga gagagcgatc
      481 ttcaagggct ttggaaagga tctggtcgtc ggcgtagaag tgggcagaca cgcgcatgtc
      541 atgccggaag gtttgaggaa tctcgtacag gtagtcgccg actctgcgca ggtcctggcg
      601 gctgatcatc gacccctcct cgctgaacaa ggcggcgcga aaaagttgca ggttgaagga
      661 tcaccctcaa cctgcaaccg atcagcgctt cagcttaatg actggactcg cagccgccgc
      721 caccggcgtc atccttcgtg cggaaggagt tgccgcagcc acagcttgag acggcgttgg
      781 gattctcgat gtggaagccg ccgcccatca ggctgtcttc gtagtcgatg ttcgcgccca
      841 caatgtacgg gaggctgcgc ggatctacga tcacgcgcag cccatgttcg gcaaacacaa
      901 tatcaacgtc gcgcgggttg ttgtcgaagg tcatgccgta ctgaaaaccg gagcagccgc
      961 ctccgcccac gaaaacgcgc aggctgtagg catcgcccaa gcccttctct tgcatgagac
     1021 cctgcaactt ttcagctgcc gactgggtca acgtaattac cggcgcctgc acctcggacg
     1081 ccgcctcttc tacggtcgta acggtcatcg aaatcctcca gaagtcaatc agccaccgat
     1141 ttggctcatc tcacgcgata gcgggatctc ctcggtctcc aggcggtggc cccatggttt
     1201 gtggtacgcc gcatccgcca tcagttggcg caatgtcgcg aaatcggcgc ccgcgcgcaa
     1261 tggggtcagc aagtcagcct cgtcatcccg caacaaacat aaacgtagtt taccatcggc
     1321 tgtcaagcgc agccgggtgc aaccttcgca gaatggctcg ctcacgctgc tgatgaaacc
     1381 gagacggccc tgcgcgcccg gcaacccata cggtctggcg gggtcatgtc catcccagcc
     1441 gggcatggca atcaacggac caaacgccac ttcaatgcgg gcacgcgttt cagtcgacgg
     1501 gaccacctgg ctgtgctgaa agtccgacac cttgccgaaa ggcatcagtt cgatgaaacg
     1561 cacatcccag ggatgctcaa aggtgagacg ggccaggtcg accacgtccc cgtcgttgaa
     1621 accgcgcgtg acgacgcaat tgatcttgat cggctcaagc ccggctgctt ccgccgcaat
     1681 cagtccctgc cagacatctt ccaggtcgcc gcgccgggta atctggcgga atctcgcggg
     1741 atccaacgaa tcaatactga tgtttacctg gtccaggccc gcctccgcca ggggagcagc
     1801 caactcagcc aggcggacgc cgttggtggt catcgtaagc cgccggatgc ccggtaccga
     1861 cttgatcccg cgcaccaaat ccaccacgcc gcggcgtacg gtgggctcgc ctcccgtcag
     1921 gcggattttg tccaccccca ggctggccgc cacccgcaca agcagcagca gttcctcgta
     1981 ttgcaggagg ctcgcctgct tgcggaagac catgtcctcg ggcatgcaat acacgcagcg
     2041 cagattacag gcgtcggtca gcgaaacgcg cagataatgc aggtgccgcc cataggaatc
     2101 atacatcgcg cccgggcgat caaccagggt gatgaaggac atcgaattct ctttggtttt
     2161 aggccttctc ggaatgcgcc accggctgga gcagcagcac ataagcacag cggggagagc
     2221 cgtctacgat ccgagactct cggcgcaccc cttcgggcac caggcgctgc atcatcgtgc
     2281 ggtccatctc acacagctcg gggttctgct ccgaaacacc ggaatacggg cagttgcaag
     2341 tgaaaatgcg atagtagccg tcagtcactt cccaacgcgc cgtgtaaccc ttgccgctca
     2401 gaaactctgc gacctggttc aaccggtcct cgacagcctg gtccggcctg gcgagaggag
     2461 cgtcctggac cgtcttgtcg gccaggcgca gcagcactcc ctcgagctcc ttggccggca
     2521 gcaatctctt catggcggac agcatctcgt tcgccagcac ttggtggcgc tgggggaaaa
     2581 gcttggacgc ctgctgcgtc aaggaataga cctggctcgg gcgtccggcg ccattgcgcc
     2641 gccgcacccc ggcagactcc acaaggtcct caccgaccag gatgtccagg tgatggcgca
     2701 cggaaacctg agccatgcct agcttcccgg ccagttccgc aacggtagcc gtgccgttct
     2761 ccttcaagat ctcgacgatg tgattgcgga ctgtctgcat attctcctcc ttgtgcggcc
     2821 cggagccctg atttggcctg aagctaccac tgcaaacaag cccgatcggg tcgccgccca
     2881 tccttgtcac tgatataaga atcttatcac ggctggcaat ctctatcaat tcttaaatac
     2941 ttatcactct aatattatta tcactattct accacgcccc tggggaaaac gcaagtagca
     3001 atccgtacaa agtcacaggc ggccagctgg gggactcccg tactttctac gtagcctcat
     3061 tcacgcatat cagtattatt acgcaagtcg ttcattttgt aaaattgacg caacccaatt
     3121 gattagaatt aaaactgcag ctaaatcctg cattaaacgg cggctgcgag cgcgatgcga
     3181 gattgcaccc ctgacgaaaa agcctggcgt tgggtttggc cggggccgcg aggtggtgct
     3241 atactggcga ccccatggca accatcactg gcgctttccc ggtcctgttc ctgatcgccc
     3301 gtccggccgc cggcaaaagc gagatcatcg actatctgaa gcgcgcggtc ttcgagaacg
     3361 aggacgacgt caccactacc cgtgggaagg cgctcggcga ccggctgcag gagacgatgg
     3421 gacctttatg gacattcttc gaagcttttc ataacaatcc ttaagagaaa tgtgccgcgc
     3481 agaaatttaa ggttcgtgac aaatatccac tgcaatacga gcgggctgtg gtcctgcgcc
     3541 gacacaccac gaccggtcgc gtagaacagc agtgcgtcat gcggccgttc tacataagct
     3601 cagagccccg gcgcagttct ccggcaactg gcggctgtac ggccgggggg agtgctgcta
     3661 gaatccgctg taggaaagtg aattctgccg gcaaaacggc atgacgaaat cggtttcttt
     3721 tctggcccgg aggccagaat cctccacatc tcctgatgac ggtcgtaggc cgtcgttgcg
     3781 cccttggctg acggcgctgc aaatggctgg cgatggtgcc agggtgtttg aactcgatgg
     3841 tccgcagcca cggaagtgca ctgggcgaac tgggtgaacg gttagtagca attgaggttt
     3901 cacgccaatt agtggcgcgt ctacgccacg gtggtttttg cgtcctcgaa cgccggctgg
     3961 gctgggcctc ctgacaggga tgcaacagca ctccggccgg cgagaataag gatgaataac
     4021 gtgtacgttg ccgatatgcc gggtgcgctg ccgccgaagg aagcctggga ggtggcccgc
     4081 agccaactgc gcctgcagat gagcaagagc acctacgaaa cctgggtgcg cgacagcgtc
     4141 tacatggcgc acgaggacgg cgcgtttgtg atcggtgttc cgaatgccta tgccaaggat
     4201 tggctctcca tgcgcttgcg gccgctgatc aagcggaccc tgagcagcat cgtgggccac
     4261 tctgtggatg tgacattcgt ggtgcgatct gtgcccctcg ccgaatcatt aaagcccgcc
     4321 ggcggtctcc tgttggacat ggacaaatcc cgccaggcgg at
//
LOCUS       DATCGS010000020         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_1932, whole genome shotgun sequence.
ACCESSION   DATCGS010000020 DATCGS010000000
VERSION     DATCGS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VIL39_00325"
     CDS             <1..1305
                     /locus_tag="VIL39_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5479901.1"
                     /translation="SGSSAVNNPLAILQTLDRHLDHPSIRTPNLDRMAAQGMRFTDFY
                     VAACVCTPSRAALLTGRLPIRSGMSGDLQHRVQTKNSPGGLPPEEITIARALKTKGYA
                     TQAIGKWHLGHYPQNMPTSHGFDAFYGLRWSNDMEPARGIPRDASSSLNPKVEWWNAA
                     LVRNDKIIEQPTDLHTLTKRYTEEAVHFIQQNKKKPFFLYFAHTYPHVPLFASKQFQQ
                     TSPRGLYGDVVEELDWSVGQVLDTLRKQGLADNTLVFFTSDNGPWLVKDRVGGCAGLL
                     FGGKGSTWEGGMREPGIAWWPGKIQAGVINREMACSMDLFSTCLTLAKVPIPADRIID
                     GVDMTPMLLGKGPGTRNLMIYYNGDEVYAVRKGPYKAHYTTFSGYGKDTPLKHEPPLL
                     FDLPDDPGERFDVAAEHPEVVADIQREVEKYRATLVPGKRQY"
     gene            complement(1486..1842)
                     /locus_tag="VIL39_00330"
                     /pseudo
     CDS             complement(1486..1842)
                     /locus_tag="VIL39_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            2180..>2384
                     /locus_tag="VIL39_00335"
     CDS             2180..>2384
                     /locus_tag="VIL39_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479902.1"
                     /translation="MSISIRRWLRADLLTIQRLLLETWLDAYAPFIPREDLAGYLNAQ
                     YSQAKLDALFEDPDVTGLVAEADG"
BASE COUNT          451 a          746 c          723 g          464 t
ORIGIN      
        1 agtggctcaa gcgccgtgaa taatccgctc gccatcctgc aaacgctgga ccgccacctc
       61 gatcatccga gcattcgcac gcccaacctc gaccgcatgg ccgcacaggg catgcgcttc
      121 accgatttct acgttgccgc ttgtgtctgc acgcccagcc gggcggcgct gctcaccggc
      181 cgcctgccca ttcgcagcgg catgtccggc gatctgcagc accgggtgca gacgaagaac
      241 tcaccgggcg gcctgccgcc cgaggagatc accatcgcgc gcgcgctcaa gaccaagggc
      301 tatgcgaccc aggccatcgg caaatggcac ctgggtcatt acccgcagaa catgcccacc
      361 agccacggct tcgacgcctt ctacggcttg cgctggtcca acgacatgga accggcgcgc
      421 ggcattccca gggatgcttc ctccagcctc aaccccaaag tcgaatggtg gaatgcggca
      481 ctggtgcgca acgataagat catcgagcag ccgaccgatc tccataccct gaccaagcgc
      541 tacacggagg aggccgtgca tttcattcag cagaacaaga agaagccgtt cttcctctat
      601 ttcgcgcaca cttacccgca cgtcccgctc ttcgcctcga agcagttcca gcagaccagc
      661 ccgcgcggct tgtatggcga tgtggtggaa gaactggatt ggagcgtcgg ccaggtgctg
      721 gacacattgc gcaagcaggg gttggccgac aacacactgg tcttctttac gagcgataat
      781 gggccgtggc tggtgaaaga ccgtgttggc ggctgcgcgg ggcttttgtt cgggggcaaa
      841 ggcagcacct gggaaggggg catgcgcgag ccgggcatcg cctggtggcc gggcaagatc
      901 caggccggcg tcatcaatcg ggagatggcc tgctcgatgg acctgttcag cacctgcctg
      961 actttggcca aagttcctat ccccgccgac cgcatcattg acggcgtgga catgaccccg
     1021 atgctgctgg gcaagggccc cggcacgcgg aacctgatga tctactacaa cggcgatgaa
     1081 gtgtatgccg tgcgcaaagg cccgtacaag gcccactaca ccaccttctc cggctacggc
     1141 aaagacaccc cgctgaagca cgagccgccg ctgctgttcg acctgccgga tgatcctggc
     1201 gaacgctttg acgtggccgc cgagcatccc gaggttgtcg ccgacattca gcgcgaggtg
     1261 gaaaagtacc gtgccaccct ggtgcccggg aaacggcaat actgaggagt attgagtgcg
     1321 ggctcacccc gaccggagcg cggacattcc tgtccgcagc agcgtaagga ggccaacacg
     1381 gcctatagac ccagaagtcg caggcaagcc cggggcttgc tgcggacaag aatgtccgcg
     1441 ctccggtgct gccgggaagc tccagccgct ttagacgcgg ggggcttact ggatgcctag
     1501 aagttccacc tcgaatacca gcgtgctgtt cgctgggatc ttcgggcttg ggctgttcgc
     1561 gccataagcc aggtcgggag gaatctcgaa cttgaacttg tcccccacct tcatgagttg
     1621 caggccttcg gtccagccgg aaatgactct gttcaacggg aagctgatcg gttgtccgcg
     1681 ttccacggaa ctgtcaaaga cggtgccgtt gagcagcgtg ccgtggtaat gcacattcac
     1741 cgtgtccgtg actttcggcg agatggttcc cgtgccctgt ttcagcacct ggtacttgag
     1801 tccgcttggt gttgttttca tttcgttctt ctgaccgcgg tcagcctgcc cgcccggggc
     1861 ggctttgacc gcgggtttat cagcggccgg ttccgctggt ttgctgcaac ccagcaggca
     1921 aatagccgct gccaatgtca ccgagagtcg tgttatcatg cttcaataat ccggtagttc
     1981 ctgatcaacg cggcggtagc ttccgccatg cgcgacccgc cgtcaagcca tgtgagcgac
     2041 agccatgtga gcgacgcttg cgacttgctc tccgcgggct tgagcgttag aagttggctt
     2101 gtatgtattc ctttgctggt ggacgtaata acttcgtcgc gcgtcagccg gcattcatgc
     2161 catttaccag ggaggcccaa tgagcatctc gattcggcgc tggctgcgcg cggacctgct
     2221 aacgatacaa cggctgctgc tcgagacatg gctggatgcc tacgctccat tcatcccgcg
     2281 cgaggacctg gccgggtacc tcaacgccca gtattcgcag gcgaagctcg acgcgctgtt
     2341 cgaggacccg gatgtgactg ggttggtcgc cgaggcggat gggg
//
LOCUS       DATCGS010000021         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_546555, whole genome shotgun sequence.
ACCESSION   DATCGS010000021 DATCGS010000000
VERSION     DATCGS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546555"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /gene="rpsH"
                     /locus_tag="VIL39_00340"
     CDS             <1..275
                     /gene="rpsH"
                     /locus_tag="VIL39_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5479903.1"
                     /translation="KQEGYITDFAVEGKVPRTIKVKLKYQGKKSIIEGLRRISTPGLR
                     RYVGATEIPRVRGGLGVAVLSTSEGLMTDVQARRKKIGGELICYVW"
     gene            377..913
                     /gene="rplF"
                     /locus_tag="VIL39_00345"
     CDS             377..913
                     /gene="rplF"
                     /locus_tag="VIL39_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5479904.1"
                     /translation="MSRIGKQPIAIPPKVKVEVKGQKVFVEGPKGKLDWELPRRTSLK
                     VEGGKIVVSREGDDARAKALHGLSRALVNNMVHGVSEGFVKKLEIQGVGFKAAVAGNN
                     VNMSLGYSHPIVYPIPAQVKVTVEENTKITIEGPDRQSVGQVASELRSFYPPEPYKGK
                     GVRYSDERVIRKEGKTVQ"
     gene            993..1358
                     /gene="rplR"
                     /locus_tag="VIL39_00350"
     CDS             993..1358
                     /gene="rplR"
                     /locus_tag="VIL39_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5479905.1"
                     /translation="MRTEQKHKLALRRRWRIRRKISGTKDRPRMSVCFTNKNIHIQFI
                     DDAAGVTLAAASTTSKAIPDRDKLAANAASAKTLGTLAAQAALGKGIKQVVFDRGGAR
                     YHGKIKALADAAREAGLQF"
     gene            1365..2030
                     /gene="rpsE"
                     /locus_tag="VIL39_00355"
     CDS             1365..2030
                     /gene="rpsE"
                     /locus_tag="VIL39_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY5479906.1"
                     /translation="METNTVPEPTKVESGEHRPARGPARGRRTRPEPAPTFDPQSGQQ
                     LTEKVVFINRSAKVVKGGRRFNFSALIVVGDKHGRVGVGLGKAGEVADAIRKGGEMAR
                     SQMVNVSLKDATIPHEVFTHYCGAKVLLRPASPGTGIIAGKTVRAVLELAGVKDVLTK
                     SLGSKNAANVVKATLQALLSQRLREDIYSRRGVEIKKAQVPEVPESTSPTPSLPTTPP
                     PTA"
     gene            2037..2525
                     /gene="rplO"
                     /locus_tag="VIL39_00360"
     CDS             2037..2525
                     /gene="rplO"
                     /locus_tag="VIL39_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5479907.1"
                     /translation="MRLHDLKPRPGARHRRKRLGQGESSGHGKTSGRGGKGQTARSGS
                     SIRIGFEGGQMPLIRRIPKRGFNNARHTTRYLPVNLEALNRFEDGARVDEAALRSAEL
                     ANGRAAGIKILGEGELTRTLTVIAHAFSASAKAKIEAKGGKWELTAPKSPKAEKSAPA
                     KP"
     gene            2598..3992
                     /gene="secY"
                     /locus_tag="VIL39_00365"
     CDS             2598..3992
                     /gene="secY"
                     /locus_tag="VIL39_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5479908.1"
                     /translation="MLSAIYTTFCNCFKIPELKSRILFTLVVLAICRLEAVVRIPGLD
                     GTVLSNYFNTQAGSAAGGGMLGMYSMFTGGALEHCAVGALGIMPYISATIIIQLLTAV
                     VPTLSKLAREEGGRTKIIQYGRYLTVLLCLGQGMVMAIGWENPEKLFPQWNPAERLVL
                     YSDAHLWFYRLQTLMMLTTGTMLLMWLGEQITERGIGNGVSLVITIGILARLPQAAVS
                     LKDMFFPDGGVEARFNFGHAIALVLLLAAVIAGVIAITQAQRKVPVQYAQRAVGRKVY
                     SGGTSFMPLRVNYSGVMPIIFAQCILMFPQKIFQFLGQSFNVKFFREIAVALNEGAIL
                     YMFVYGLMILFFSYFWVATQFNELQIADDLKKNGGYIPGVRPGQATSDYLHNAMSRIT
                     LAGAIFLTIIALIPIWLYRELQIPYAVATFFGGTSMLILVGVLLDTMRQMETHLLMRH
                     YDGFLKKGRLRGRF"
     gene            3992..>4453
                     /locus_tag="VIL39_00370"
     CDS             3992..>4453
                     /locus_tag="VIL39_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY5479909.1"
                     /translation="MIIVKSERDLEAMRPACAVAGTVLEEVAAFVQPGLTTRDVDEFA
                     ASRIKHHGGKSAFLGYRKYPCQICISVNDQVVHGMAGPRTLRFGDIASLDVGVVYNGF
                     VGDTARTVAVGGCDVLAQRLMDVTERALHEGIAQAVAGKRVADISRAVQNHV"
BASE COUNT          942 a         1194 c         1407 g          910 t
ORIGIN      
        1 tgaaacagga gggttacatt accgattttg ctgtggaagg aaaggtgccc aggacgatca
       61 aggtgaagct gaagtatcag ggcaagaaga gcatcatcga gggcctgcgc cgtataagca
      121 ctccgggctt gcgccgttac gttggcgcga cagaaatccc gcgcgtgcgc ggcggtttgg
      181 gggtggctgt cctctcaacc tcggagggcc tgatgaccga cgtgcaggcg cgccgaaaga
      241 agatcggggg agagttgatt tgttacgtgt ggtaagaaga tgaaacggag tataggagcg
      301 ttgaagtgct ggagttgtgc tggtcggcag cacctcaccg gcccggcgct ctaccgctcc
      361 atcgcagttt gattttatgt cacgaattgg aaagcaaccg attgccattc ctcccaaggt
      421 gaaagtggag gtcaaaggcc agaaggtttt cgttgagggg ccgaaaggca agctcgactg
      481 ggaactgccg cgccgcacca gcctgaaggt ggagggcgga aagatcgttg tcagccgcga
      541 gggcgatgac gcgcgggcca aggcgctgca tggcctcagc cgcgccctgg tcaacaatat
      601 ggtccacggc gtcagcgaag gcttcgtcaa gaagcttgaa attcaaggcg tcggcttcaa
      661 ggccgccgtg gcgggcaata acgtaaacat gagtttgggc tattcgcatc ctatcgtgta
      721 tccgattccg gcccaggtca aagtgacggt ggaagaaaac accaagatca ccatcgaagg
      781 accggaccgg cagtcggttg gccaggtggc ttcggagctc cgcagtttct acccgcctga
      841 accttacaag ggcaagggcg tgcgctactc ggatgaacga gtaatccgca aggaaggcaa
      901 gaccgtgcag taagtaatgc ctgacgcgtg aaacgtgaaa gacggacact tgatacttta
      961 gcagttcacg gccaaatccg tgacaaattg agatgagaac tgaacagaaa cataaactgg
     1021 cgctccggcg ccgctggcgc attcgtcgga agatcagcgg cacgaaggac cgtccgcgga
     1081 tgagcgtttg cttcaccaac aagaacatcc atatccaatt cattgatgac gcggcggggg
     1141 tgacgctggc ggcggcttcg acgacgagca aggcgatccc ggaccgggac aagctggccg
     1201 ccaatgccgc gagcgccaag acgctgggca ccttggcggc acaagccgcc ttgggcaagg
     1261 gaatcaaaca ggtggttttt gaccgcggcg gtgcgcgtta ccatggtaag ataaaagcct
     1321 tggccgacgc agcgcgcgaa gcaggtctcc aattctaacg cataatggaa acgaatactg
     1381 tgcctgaacc aaccaaagta gagagcggcg aacaccgccc tgcacgcgga ccagcccgcg
     1441 ggcgccgcac ccgcccggag ccagcaccca cttttgaccc gcagtccggg cagcaactga
     1501 ccgaaaaggt tgtgtttatc aatcgttccg ccaaagtcgt caaaggggga cgacgcttca
     1561 atttcagcgc gctgattgtc gtcggcgaca agcacggccg agtgggggtt ggtttgggca
     1621 aggccgggga agtggccgat gcaattcgca agggcggcga aatggcccgc tcgcaaatgg
     1681 tcaatgtgtc gttgaaggat gcgaccattc cgcacgaagt gttcacgcat tattgcggcg
     1741 caaaagtcct gctgcggccg gcttcgccgg gcacgggtat tatcgcgggc aagacagtgc
     1801 gggccgttct cgaattggca ggcgtcaaag acgtcctgac caagtcactc ggttccaaga
     1861 atgccgccaa tgtggtcaaa gccacgctcc aagcgctgtt gagccagcgt ctccgcgaag
     1921 acatttattc aagacggggc gtggaaatca agaaggcgca agtgcctgaa gttccggaat
     1981 cgacatcgcc gacaccttcg cttccgacga cgcctccacc aacggcttag ctttttatgc
     2041 gattacatga tctcaaacct cgtcctggcg cccggcaccg tcgtaaacga ttaggccagg
     2101 gcgaatccag cggccacggt aaaaccagcg gccgcggcgg caagggccag acagcccgct
     2161 cgggtagctc gattcgcatc ggttttgaag gcggtcaaat gccattgatc cgccgcattc
     2221 ccaagcgtgg atttaacaat gcacgccata ccacgcggta tctgccggtg aacctggagg
     2281 cgctcaaccg cttcgaggac ggcgcgcgtg tggacgaagc cgccttgcgg agcgcggagc
     2341 tcgcgaacgg gcgcgcggcc ggcatcaaga tcctcggaga aggggaactg actcggacgc
     2401 tcacggtgat cgcgcacgcg tttagcgcct cggccaaagc caaaatcgaa gctaaaggag
     2461 ggaagtggga acttaccgcc ccgaagagcc cgaaggcgga aaaatctgcg cctgccaagc
     2521 cttgaatctg atcatgcgct cgaaagcaac tcttgggtta accgttcggg tttcggactt
     2581 cggaatctaa taccgctatg ctttctgcca tctacactac cttctgcaat tgctttaaga
     2641 ttccggagct taagtcccgg attctgttca ccttggtggt gctggccatt tgccgcctgg
     2701 aggcggttgt gcgcatcccg gggctggatg gcactgtgct gtcgaattat ttcaacaccc
     2761 aggccggctc tgcggcgggc ggggggatgc tgggcatgta cagcatgttc accggcggcg
     2821 ccctggagca ttgcgcggtg ggcgctttgg gcatcatgcc ctacatcagc gcgaccatta
     2881 tcattcaatt gctgacggcg gttgttccca ccttgagcaa gcttgcccgc gaagagggcg
     2941 gtcgcaccaa gattattcag tatggccgtt acctgaccgt gctcctctgc ctggggcagg
     3001 gcatggtgat ggcgattggc tgggagaacc cggagaagct tttcccgcag tggaacccgg
     3061 cggagcggtt ggtgctctac tccgatgctc acctttggtt ctaccgcctc caaacgctca
     3121 tgatgctgac cacagggacg atgctgctga tgtggctagg cgagcagatc accgagcgtg
     3181 gcatcggcaa cggtgtttca ctggtcatca ccattggtat tctggcgcga cttccgcaag
     3241 cggcggtatc tctcaaagat atgttcttcc ccgacggcgg ggtcgaggct agattcaact
     3301 tcgggcatgc catcgcgctg gtgctgttgc tggccgctgt gattgccggg gtcatcgcca
     3361 tcacccaggc gcagcgaaag gtaccggttc aatacgcgca gcgggcggtg ggccgcaaag
     3421 tctattcggg cggcacctcg tttatgccgc tgcgagtcaa ctactcaggt gtgatgccga
     3481 ttatttttgc ccagtgtatt ctgatgtttc cgcagaagat cttccagttc cttggacaaa
     3541 gcttcaacgt gaagttcttt cgagagattg cggtcgcgct caacgaaggg gcgattctct
     3601 atatgtttgt ttacgggttg atgattctct tcttctccta tttctgggtg gccacccagt
     3661 tcaacgaatt gcagatcgcg gatgatttga agaagaacgg cggctacatt cccggggtgc
     3721 ggcccgggca ggcgacgagc gattatctgc acaatgccat gagccggatt acactggcgg
     3781 gtgcgatctt tctgaccatt attgccctga ttccgatctg gttgtatcgg gaactgcaga
     3841 ttccgtatgc ggtggccacg ttcttcggcg gcacgagcat gttgatcttg gtcggtgtgc
     3901 tgctggatac gatgcggcag atggaaaccc atctattgat gcgccattac gacggttttc
     3961 tgaagaaggg gcggctgcga ggccggttct gatgatcatc gtgaagagtg agcgagactt
     4021 ggaagcgatg cgaccagcct gcgcggtggc cggcaccgtc cttgaagaag ttgccgcctt
     4081 tgttcagcct gggttgacta cgagggacgt ggatgagttt gcggcctcgc ggatcaagca
     4141 tcatggcggg aagagcgcgt ttctgggtta ccgtaagtat ccgtgtcaga tctgcatctc
     4201 ggtgaatgat caggtcgttc acggcatggc gggcccccgc acgctgcgtt tcggcgacat
     4261 cgccagtttg gatgtcgggg ttgtttacaa cgggttcgtt ggggacacgg cacggacggt
     4321 cgcggtgggc ggctgcgatg tgctggctca gaggctcatg gacgtcacgg aaagagcctt
     4381 gcacgagggc atcgcccagg cggtagcggg caagcgggtg gctgatattt cgcgggcggt
     4441 acagaatcat gtg
//
LOCUS       DATCGS010000022         6877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_51774, whole genome shotgun sequence.
ACCESSION   DATCGS010000022 DATCGS010000000
VERSION     DATCGS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51774"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIL39_00375"
     CDS             <1..384
                     /locus_tag="VIL39_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5479910.1"
                     /translation="STFLGHPVGCAMALAEIKEIKARKLVQRSAALGEILLASLSILD
                     PRPSTLHVTPRGLGLLAGLELCLPHGAPATEATLKLVKAMLKRGFILLPEGEHSNVIG
                     FTPPLTISPNQLQTAVNALEKLLAL"
     gene            381..1229
                     /gene="rsmA"
                     /locus_tag="VIL39_00380"
     CDS             381..1229
                     /gene="rsmA"
                     /locus_tag="VIL39_00380"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5479911.1"
                     /translation="MNLNEMKQVLAERGIQLTKSLGQNFLHDANQLRRIMAVAELKQT
                     DRILEIGPGLGPLTEILIEQVGEVLAIEKDARLVEVLKQRFQTETGGTPVLRLLHDDA
                     LDYLKREARDLSGWKMVANLPFSVASPLLVELALSARGPQRMVGTLQVEVAKRLHAEP
                     GAPDYGVLTLLVQLNYEPQPWFKIPPSCFFPEPDVDSACVCLVRREPPPLTNDQRRVF
                     VRLVKRGFSQRRKMMLKLLKQEWPQPRLAQEFERLNLSPQIRAEKVSLDQFIQLARNL
                     SEAPSV"
     gene            1226..1732
                     /locus_tag="VIL39_00385"
     CDS             1226..1732
                     /locus_tag="VIL39_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5479912.1"
                     /translation="MSREIFDVVNERDEVIGQQERSEVHRLGLMHRAAHVLVFNAAGD
                     VFLQKRSMTKDRQPGLWDSSASGHVDAGEDCDACAARELGEEIGLQLSTAPQRLFKFA
                     ACAETDQEHVWVYRCEAEGPFRLHPEEIERGGWFAPAAVTRWMAERPEDFSGALLLIW
                     KKVNRELL"
     gene            1932..2573
                     /locus_tag="VIL39_00390"
     CDS             1932..2573
                     /locus_tag="VIL39_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY5479913.1"
                     /translation="MNNFLVPMVVEQTGRGERGYDIYSRLLVDRIVFLGTPVDDMVAN
                     LIIAQLLFLQMSDPKKDIHLYINSPGGSVTSGLAIYDTMQFLTCDVNTYCIGQAASMG
                     AVLLCGGTKGKRYALPNSNIMIHQLLGGAEGPASDVEIRVRYMLKLKQRLNSIVAKHS
                     GRSLEQVEKDTDRDNFMSPEEAKAYGLVDEVVQSRKEIPGLAENATSLADKKE"
     gene            2579..3871
                     /gene="clpX"
                     /locus_tag="VIL39_00395"
     CDS             2579..3871
                     /gene="clpX"
                     /locus_tag="VIL39_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY5479914.1"
                     /translation="MARPTNLTLCSFCGKSHAEVKKLIAGPGVYICDSCVILCKQVLD
                     KELAVQTRKPKQPVSIAKPAELKRQLDLACIGQEHAKKTLSVAVHNHYKRVLQEAPPP
                     PQEGEEAAPTNPHAEVEIEKSNILMIGPTGSGKTLLARTLAQILDVPFAIADATTLTE
                     AGYVGEDVENIILRLLQNADYDVKRAQRGIIYIDEIDKIARKTENVSITRDVSGEGVQ
                     QALLKILEGTICSVPPQGGRKHPQQEYIRVDTSDILFICGGAFVGLERIIQRRLGKHV
                     MGFRAVEDGLKAGTINRQEILQYVEPEDLLGFGFIPEFIGRLPMVTVLAELTESQLVS
                     ILTDPKNALTKQYAKLMAMEGVELEFSTDALHELAVQAIKKGTGARALRSLLERLMLD
                     IMYEVPSNDDILHIHINQAAVTGQSKPIIRRKHGRVAA"
     gene            4123..6093
                     /locus_tag="VIL39_00400"
     CDS             4123..6093
                     /locus_tag="VIL39_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5479915.1"
                     /translation="MLTLSGISKAYGERVLFADVLLQVNRQDRIGLVGPNGAGKTTLF
                     SIILGEESADTGTITSERGVSIGYLPQENAPVADETVIELATAITPEYMKLRRILKAW
                     ETDHPTEALHSEEIHDDVHDRFNELGGYRLEAKASQILAGLSFRNQDFERPAREMSGG
                     WVMRAHLARLLTQEPDLLLLDEPTNHLDLEALLWFQGYLKDYAGAILVISHDREFLNQ
                     LVGSIVEIRQSKLIRYRGGYDEYLQQREAYEQQQLAAFKHQEREIGRLMDFVNRFRAK
                     NTKAAQAQSKLKQIERMDKVEAPEGDDRKLVFRFPQPQRSGLRAIKLENIHHAYGEKV
                     VYRGVDFQAERGQRVVLVGPNGAGKSTLLKILAGVITPQAGTRTLGHNAKAGYYSQYR
                     VDMLQPDRTVLEEAFDTPQRLTEQFVRTLLGCFLFSGDDVFKKVEVLSGGEKSRLALV
                     KLLLDPPNLLLMDEPTTHLDLSSVDALAYALDQFEGTLIFISHDVYFIRALANHVVHV
                     NAGRLTHYSGGYQYYLDKTKAESERAGLTATGRDASNQATNQNKKGRDAVENGAATSK
                     KDQKRQEAEHRQARSRGRKAQQQIVHRLEKEIQQLEARQTELVAELEKQETYEQPGRA
                     QEINRDLMDVQHRLAELNPQWEQQATRLAALG"
     gene            6281..6571
                     /locus_tag="VIL39_00405"
     CDS             6281..6571
                     /locus_tag="VIL39_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY5479916.1"
                     /translation="MSTKLYVGNLPFDVTENDLRDMLAQHGPVNEINVIMDKGTGRAR
                     GFAFATMNTEEGAKAAILGLNGKDWKGRALTVNEARPREERPAGGGGQRARY"
     gene            6695..>6877
                     /locus_tag="VIL39_00410"
     CDS             6695..>6877
                     /locus_tag="VIL39_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479917.1"
                     /translation="MAEPSVNTVSETRQQRFERILSLEPFKGLKAILDNLSRDQVALC
                     DGVYGTNSYEDLLAKLG"
BASE COUNT         1480 a         2057 c         2104 g         1236 t
ORIGIN      
        1 agcacattcc tgggccatcc cgtcggctgc gcgatggcac tggcggagat taaggaaatc
       61 aaggcacgca aactagtgca gcgcagcgcg gcgctcggcg agatcttgct tgcctccctc
      121 tcgatcctcg accctcggcc ctcgaccctc cacgtgacgc cgcgcggctt gggtcttctg
      181 gctggtttgg aactttgcct gccgcatggc gctcccgcta cggaggccac gctgaagctg
      241 gttaaggcta tgctcaagcg cgggttcata ctgctgcccg aaggcgagca ctcgaacgtc
      301 atcggcttca ccccgccgct gaccatctcg ccgaaccaac tccagaccgc ggtcaatgct
      361 ctagagaagc tgttagccct atgaacctca atgagatgaa gcaagtgttg gcggagcgcg
      421 gcatccaact gaccaagtcg ctgggccaga actttctcca cgacgccaac cagttgcggc
      481 gcatcatggc cgtcgctgag ctgaagcaga ccgatcgcat attggagatc ggacctgggc
      541 tcgggccgct gaccgaaata ctgatcgagc aagtgggcga ggtcctggcc atcgagaagg
      601 acgccagatt ggtggaggtg ctcaagcagc gcttccagac cgagaccggc gggacgcccg
      661 tgctgcgttt gctccacgac gacgccctcg actacctgaa acgcgaagcg cgcgatttga
      721 gcgggtggaa gatggtcgcc aacctgccct tctccgtcgc ctcgcccctt ctggtggagt
      781 tggcgctgtc ggcccgcggc ccgcaacgga tggtgggcac gctgcaagtc gaggtggcga
      841 agcgattgca tgccgaaccg ggcgcgccgg attatggcgt gctcacgttg ctggtccaac
      901 tcaattacga accgcagccc tggttcaaga ttccacccag ttgctttttc ccggagccgg
      961 acgtggactc agcctgcgtc tgcctggtgc gccgtgagcc gccgccgcta acgaacgacc
     1021 agcgcagggt gttcgtccgg ctggtgaaac gcggcttctc ccagcggcgt aagatgatgc
     1081 tcaaactgct caagcaggag tggccgcagc cgcgcttggc ccaggaattt gagcgcctga
     1141 acctctcccc gcaaatccgc gcagagaagg tctctttgga tcagttcatc cagcttgccc
     1201 ggaatctaag cgaggcgcct tcagtatgag cagagagata ttcgatgtcg tcaacgagcg
     1261 cgatgaggtc atcggacagc aggagcgcag cgaggtgcat cgcctcggtc tgatgcaccg
     1321 ggcggcgcac gtgctggtgt tcaacgcggc aggagatgtc ttcttgcaga agcggtccat
     1381 gaccaaggac cggcaacctg gcttgtggga ttcgtccgcc tcgggccatg tggatgccgg
     1441 ggaggattgt gatgcctgcg ccgcgcgcga gctgggcgag gaaatcggcc tgcaattgag
     1501 cacagccccg cagcggctct tcaagttcgc cgcctgtgcc gaaacggacc aggagcatgt
     1561 ctgggtctat cgttgcgaag cggaagggcc gttccgcctg cacccggagg aaatcgaacg
     1621 cggcggctgg tttgcgcccg cagccgtcac gcgctggatg gcggaaaggc cggaggattt
     1681 ctccggagcg ttgttgttga tctggaagaa ggttaaccgc gaattgctct aattctcgct
     1741 aacaccacaa cggacagtgt cttgcgaggc cgaccgaacc caaactcctg tggtctgaag
     1801 gtcgcgctcc tgtccgacgg aggtagtgct ggttttggtc ggtgcgcgtt gtgcctgata
     1861 ctccgccgcg cacggttctt gacccgggtg ggccactcca ttaggttgca gccagagaat
     1921 atatagtatt tatgaacaat ttcttagtgc cgatggtggt tgagcagacc gggcgagggg
     1981 aacggggcta tgacatttac tcgcggctgc tggtggatcg gatcgtgttt ttgggcacgc
     2041 ccgtggatga catggtggcg aacctgatta tcgcgcaatt gctgttcctg cagatgagcg
     2101 atcccaagaa ggacatccat ctctacatca attcgccggg cggcagcgtc acctccgggc
     2161 tcgcgattta tgacaccatg cagttcctga cctgcgacgt gaacacgtat tgcatcgggc
     2221 aggcggccag catgggggcg gtgttgctgt gcggcggcac caagggcaaa cgctacgctc
     2281 tgccaaactc gaatatcatg atccaccaat tgctcggcgg cgcggagggc ccggccagcg
     2341 acgtggagat ccgcgtgcgc tacatgctca aactcaaaca gcgcctcaac agcatcgtcg
     2401 ccaagcacag cggtcgttcg ctcgaacagg tcgaaaagga caccgaccgc gacaacttca
     2461 tgagccccga agaggcgaaa gcctacgggc tcgtggacga agtcgtgcag tcccgcaaag
     2521 agattcccgg cctggccgag aacgccacga gtctggccga taagaaagaa tagccccgat
     2581 ggcccgtcca accaacctga cgctctgcag cttttgcggc aagtcccatg ccgaggtcaa
     2641 aaagctcatt gccgggccag gtgtctatat ctgcgacagt tgcgtcattc tctgcaagca
     2701 ggtcctcgac aaagagctcg ccgtacaaac caggaagccc aagcagcccg tcagcatcgc
     2761 caagcccgcc gaactcaagc gccagcttga cctcgcctgc atcggtcagg aacacgccaa
     2821 gaaaaccctg tccgtggcgg tgcacaacca ctacaaacgc gtccttcagg aagccccgcc
     2881 tccgccacag gaaggggaag aggcggcgcc aaccaacccg cacgcagaag tggagatcga
     2941 gaaaagcaac attctcatga tcgggccgac gggttcaggt aaaaccctgc tcgcccgcac
     3001 gcttgcgcaa atcctggacg tgccgttcgc aatcgcggac gcgaccacgc tgaccgaagc
     3061 gggctacgta ggtgaggacg tcgagaacat catcctgcgg ctgctgcaga acgccgatta
     3121 cgacgtgaag cgcgcccagc gggggattat ctatattgac gaaatagaca agatcgcgcg
     3181 caagaccgag aacgtctcca tcacccgcga cgtctcgggc gaaggtgtcc agcaggcctt
     3241 gttgaaaatc ctggaaggca cgatttgcag cgtcccgccg cagggcgggc gcaaacatcc
     3301 ccagcaagaa tatatccgcg tggacacgag cgacatcctg ttcatctgcg gcggcgcctt
     3361 cgtaggtttg gaacgtatca tccagcgccg cctgggcaaa cacgtcatgg gcttccgcgc
     3421 tgtggaggac ggcctcaagg ccgggactat taaccggcag gagatactgc agtatgtcga
     3481 gccggaagat ttgctcgggt tcggctttat tcctgagttc atcggccgtc tgccgatggt
     3541 gaccgtgctg gcagagctga ccgagtccca gttggtttca attctgaccg accccaagaa
     3601 cgccctgacc aagcaatacg ccaagctcat ggcgatggaa ggcgtggaac tggagttttc
     3661 gactgatgcc ctgcacgagt tggctgtcca ggcgatcaag aagggtaccg gcgcgcgcgc
     3721 gttgcgcagc ctgctggaac ggctgatgct cgacatcatg tacgaagttc cgagcaacga
     3781 cgatatcctc cacatccaca tcaaccaggc cgcggtgacc ggtcaaagca agcccatcat
     3841 ccgccgcaaa cacggccggg ttgcggcttg agttgccggc tgcggaaagt aaaggcgacc
     3901 caggctatgg ctacccgggc cgcctacaaa ccaacccact atgaaaaggc ggatggcgtt
     3961 tactcgatca ctgacccgcc agatgcaggt aaaccggcct gcaccaatgt atggtctgca
     4021 tacatcgtac caactcattg ccatcttccg ggagtcgagg attcgagaca gcgctggttt
     4081 tagactttgg atttggggcg ttgggagcta gactacccgt tcatgttgac tctgtccggc
     4141 atcagcaagg cgtacggcga acgggtgttg ttcgccgacg tgctgctcca agtaaaccgc
     4201 caggaccgga tcgggctggt cggcccgaac ggggccggga aaaccactct gttctcgatc
     4261 atcctgggcg aggaatccgc cgacacgggc accatcacct ccgaacgcgg cgtctccatc
     4321 ggctacctgc cgcaggaaaa cgcaccggtg gcggacgaga cagtcatcga actggcgacg
     4381 gccatcacgc ccgaatacat gaagctccgg cgcatcctta aggcgtggga gacggaccac
     4441 ccgaccgagg ctctgcactc ggaggaaatc catgacgacg tgcatgatcg gtttaacgag
     4501 ctgggcggct atcggctcga agccaaggcc agccagattc ttgccggcct cagtttccgc
     4561 aaccaggatt tcgaacggcc cgcgcgcgag atgagcgggg gctgggtgat gcgggcgcat
     4621 ctggcccgtt tgctgacgca agagccggac ctgctgctgc tggacgagcc aaccaaccat
     4681 ctcgatctgg aggcgctgct gtggttccag gggtatctga aggattacgc gggggcgatt
     4741 ctcgtcatct cgcacgatcg tgagttcctg aaccagttgg tggggagcat cgtcgagatc
     4801 cgccagagca agctgatccg gtaccgcggc ggctacgacg aatatctcca gcaacgcgag
     4861 gcctacgagc agcagcagtt ggccgccttc aaacaccagg agcgcgaaat cggccgcttg
     4921 atggatttcg tcaaccgctt ccgcgccaag aacaccaaag ccgcgcaggc ccaaagcaag
     4981 ctcaagcaga ttgagcgcat ggacaaggtc gaggcgcccg agggagacga ccgcaagctg
     5041 gtgttccggt tcccccagcc gcaacggagc gggctccggg cgattaagtt ggagaatatc
     5101 caccatgcct acggcgagaa ggtggtgtat cgcggggtgg attttcaagc ggagcgcggc
     5161 cagcgcgtcg tgctggtggg gccgaacggg gcaggcaaat ccaccctgct gaaaatcctg
     5221 gcgggcgtga tcacgccgca ggcgggcacg cgcacgctcg gccacaacgc caaggccggc
     5281 tattactcgc agtatcgcgt agacatgctg cagccggacc gcaccgtgct cgaagaggcc
     5341 ttcgacaccc cgcagcgcct gacggagcaa ttcgtccgca ccctgcttgg ctgtttcctc
     5401 tttagcggcg acgatgtgtt caagaaagtc gaggtgctga gcggcggcga gaaaagccgc
     5461 ctggcgctgg tgaagctgct gctggacccg ccgaacctcc tgctcatgga cgagccgacc
     5521 acgcacctgg acctgtcgag cgtggatgcg ctggcctacg cgctcgacca gttcgaaggc
     5581 acgctcatct tcatcagcca cgacgtgtat ttcatccgcg cgctggccaa ccacgtcgtc
     5641 cacgtcaacg ccggccggct gacgcattac tccggcggtt accagtatta cctggataag
     5701 acgaaggcgg agtccgagcg cgcggggttg acggccaccg gcagggacgc gtccaaccag
     5761 gccactaatc agaacaagaa aggccgggac gctgtggaaa acggcgctgc cacgtcgaag
     5821 aaagaccaaa agcggcagga agccgaacat cgccaggcgc gttcgcgcgg gcgcaaggcc
     5881 cagcagcaga tcgtgcaccg gctggaaaag gagattcagc aactcgaagc ccgccaaact
     5941 gagctggtcg ccgagctgga gaaacaggag acctacgagc agcccggtcg cgcccaggag
     6001 atcaatcggg acctaatgga cgtgcagcac cgcctggccg aactgaatcc ccagtgggaa
     6061 cagcaagcca cccggctggc ggcgctgggg tgattcaaag aggagctaaa atacttcttg
     6121 cgttcgctgc gacattgtgt agattacctg tgcttttagg caactagtcg atttcaaggc
     6181 cggtagatcc tcagttgaat ttgctttcaa tgatgatttg aaacccgatc gtcgggaaca
     6241 gtcttcgaag gagcggtccg tctaagaaac aaatatcatt atgagtacta agttatacgt
     6301 agggaatctt ccctttgacg ttaccgaaaa cgatctccgg gacatgctgg cacagcacgg
     6361 tccggtgaac gaaatcaacg tgatcatgga taaaggcact ggccgcgctc gcggtttcgc
     6421 cttcgccacc atgaacaccg aggaaggggc caaggccgcc atcctgggct tgaatggcaa
     6481 ggattggaaa ggccgcgccc tgacggtgaa cgaggctcgt ccccgtgagg aacgtccggc
     6541 cggcggcggc ggacagcgcg cccgctacta gttcgtccgg gtggaatgat catcgcagtg
     6601 ggattctgct ccgcagaatc ccactcgcgt ttttgagttt cgcagccgct aattggcgct
     6661 cgcgactcga cgctctaccc gcaaccgaag caatatggca gaaccttcag taaacacagt
     6721 cagtgaaacg aggcagcagc ggtttgagcg cattctaagt ttggaaccct tcaaagggct
     6781 caaagcaatt ctcgataacc tatcccgtga ccaggtggca ctctgcgacg gagtgtatgg
     6841 cacgaattct tacgaagacc tgctggccaa gctgggc
//
LOCUS       DATCGS010000023         5824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_348798, whole genome shotgun sequence.
ACCESSION   DATCGS010000023 DATCGS010000000
VERSION     DATCGS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348798"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VIL39_00415"
     CDS             complement(<1..316)
                     /locus_tag="VIL39_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY5479918.1"
                     /translation="MTKAGFWKTDWFLGVAVVISVVLFNRLSDLIPSLERKAYDLGVV
                     ATSRAPSDKIAVIAIDEQSLANLGRWPWSRDALAKMTDSLSAAKAKGIAYTVLFSEPQ
                     VDV"
     gene            complement(806..1726)
                     /locus_tag="VIL39_00420"
     CDS             complement(806..1726)
                     /locus_tag="VIL39_00420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5479919.1"
                     /translation="MRIAIIGQQDFGKAVLEAFLARGDEIAAVFCAPEKEGARPDALR
                     AAAEEKGLKVYQFKSLRAPEAAQALAEAKADIGIMAFVLQFAPQEFVSIPAHGTIQYH
                     PSLLPKFRGPSSINWALIKGETETGLTIFRPSDGLDEGAVILQKKTPVSADDTLGTAY
                     FDRLFPMGVAAMLAAADLVVAGKHKEAIQDETRASYEGWCREGEARINWASHVDFIHN
                     LIRGCDPAPGAWTTHNGEKLQLFGSKKHLVRTFGAVKGKIGEVTAISASSMFITAQGG
                     QIEVAKGKLGSGKKVSPADLGIAAGTILGS"
     gene            complement(1771..3042)
                     /gene="frc"
                     /locus_tag="VIL39_00425"
     CDS             complement(1771..3042)
                     /gene="frc"
                     /locus_tag="VIL39_00425"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY5479920.1"
                     /translation="MEALKGVRILDMTHVQAGPTCSQLLAWMGADVIKFENPQGDATR
                     GQLRDIPNADSLYFTMLNCNKRSITVNMKTAEGKAVFVDLLQKCDIIMENFGPGVLDR
                     FGFSWEKIHEINPRIVMGSIKGFGSSGPYSEFKAYENVAQAMGGAMSTTGVPDGPPFV
                     TGAQIGDTGTGLHLAIGLLAALRQAEKTGQGQYVEVAMMDGIMNLCRVKWRDHQRLTR
                     GPMPEYSVGTHQGMGAVPRGGNDSGGGQLGNAIRCKPGGTNDFIYIVVQEAVWAGLAK
                     LIGAELGMSDLATNPKFATIGERRRFQNDMWGIIGEFALKHTKREVMGILNEINVPCG
                     PVMSTEDLANDEHVRGREMYVELDHPQRGKWYNIGMPIKLSASPAKIKRSPLLGEHTD
                     EVLKEVLGYDDAKRTTLKKAGAFSLPPKQAK"
     gene            3383..4000
                     /locus_tag="VIL39_00430"
     CDS             3383..4000
                     /locus_tag="VIL39_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5479921.1"
                     /translation="MSSIQSHPQHNTIRLQLRQSLVLKDMPLAAWAELEPQLEIADFG
                     KGDLLVHQGHSAMEQFFILDGILKRVVSDPKGKEMILRFAAENDMDTSYAAWRLGTPM
                     PYSIVAVTKVRVAKLSMQHWVEFLECHSELKARFEYEVMSLMSEVMAHTITLHLLDAP
                     GRLSRFKRKHPELLDRIPKKELASYLNLTPETLSRLKKRRQPGAP"
     gene            complement(4288..5637)
                     /gene="oxlT"
                     /locus_tag="VIL39_00435"
     CDS             complement(4288..5637)
                     /gene="oxlT"
                     /locus_tag="VIL39_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY5479922.1"
                     /translation="MSNESTAQIDLAKQTFGPIVGNRWIQLAAGVVAMVVISNFQYAF
                     TLFTPGLKQQFPTVPYSDIALIFSLFILFETWPVPLAGYFIDKFGIRNLMLIGAILVF
                     LGWVLGGMFATSVFHLYIYYGVLAGTGCGIIYIATVANAVKWFPDKRGLAAGLTAAGF
                     GGGSALTLIPISGSIASMGWAGAMTAWGIGQGVVAIAMALILHHPPKGWLPKDWVQPK
                     AVAQTRREFSASQMLSQKEFYIMYFMFLMVCTGGLMTTGNMSQIAKSLNVFDAKVMGI
                     AIVPFVATIAGVTNAFARIMWGSVSDRFGREYTMAFAFALEGVLIFLMTQIMGQPIAF
                     MILMPFVFLAWGEIYALFSAITGDVFGPKNASGNYGILYTAKGLASIMAGWGAAAVAA
                     HFAGSFSVPYYIAAVFDLIAAALALFVLRPIIRKRIAGEVAGEVKGPVFAATAGTTR"
BASE COUNT         1194 a         1800 c         1627 g         1203 t
ORIGIN      
        1 cgacatcgac ctgcggttcg gaaaacaata cggtataagc gatccccttc gccttcgccg
       61 ccgacaggct atcggtcatt ttcgccagcg catcgcgcga ccagggccag cggccgagat
      121 tggcaaggct ctgctcatcg atggcgatga cggcgatctt gtccgatggc gcgcgcgaag
      181 tggcgacgac accgaggtca taggctttgc gctcgaggct cggaatgagg tcgctcaagc
      241 ggttgaacag aacgacggaa atgacgacgg cgaccccgag aaaccagtcc gtcttccaaa
      301 atcccgcctt tgtcattccc ggccccctgc gcacctgatc tttttctgct tttgcctggc
      361 tatctgcccg atagtcaagg cattttacga cattagcctc ggcgcttgtg ctatttccgc
      421 tgtggcaaaa cgacagcgcg cggcgcctcg cgctgagagg cgtctggcat caaggtcccg
      481 cgtcgcctcg acgcctgatt caggttcccc tgtttgctcg ctaccgcttg acccagcaca
      541 gcttgggtaa gtcctattta taaacagggc gataatggtc attcttgcag ttaaatatca
      601 gattagtcaa atgttttttg cgaattcatc tgatgcaggc atcagccgat ggcgcaggtg
      661 gcaggcgaaa acaggcaggg gagccaggac gaccctggcg aaaaaaagaa aacccttgcc
      721 gcaggacaag ggttttagtt ccggcataac gcgcttcgcg cattagcctc cactgaaact
      781 tcgttttctg attgtgagag aacgttcaac tgccgagtat cgttcccgcg gcgatcccca
      841 gatcggccgg tgaaactttc ttgccgctgc ccagcttgcc cttggcaacc tcgatctgtc
      901 cgccctgagc ggtgatgaac atgctgctgg cgctgatcgc ggtgacctcg ccgatcttgc
      961 ctttgaccgc gccgaaagtg cgcaccagat gcttcttcga gccaaacaac tgcagcttct
     1021 cgccgttgtg cgtggtccat gcacccggcg cggggtcgca gccgcggatc aggttgtgga
     1081 tgaaatcgac gtgactggcc cagttgatcc tggcctcgcc ctcgcggcac caaccttcgt
     1141 agctagcccg ggtttcgtcc tgtatcgctt ccttgtgctt gcctgcgaca accaggtcgg
     1201 ccgccgcgag catggctgcc acgcccatcg ggaacaggcg gtcgaagtag gccgtgccga
     1261 gcgtatcgtc ggcgctgacg ggagttttct tctgcaggat cactgcgcct tcgtccaggc
     1321 catccgacgg ccggaaaatg gtgaggccgg tttcggtctc acccttgatg agggcccagt
     1381 tgatcgagga ggggccgcgg aacttgggca gcagcgacgg gtgatactgg atggttccgt
     1441 gcgctggaat gctgacgaat tcctgcggcg cgaactgcag tacgaacgcc atgatgccga
     1501 tgtctgcctt ggcttcagcc agcgcctgcg ccgcctcggg ggcacgcaag gacttgaact
     1561 ggtagacctt gagccccttc tcctcggccg cagcgcgcag ggcgtcgggc cgcgccccct
     1621 ccttctccgg cgcgcagaac accgcggcaa tctcgtcccc gcgcgcgaga aaggcctcca
     1681 gcacagcctt gccgaagtct tgctgaccga taattgcaat acgcatgatg tcgtccttta
     1741 gcgaaaaccg aatccctcgt tggcgtacta ctacttcgct tgcttcggcg gcaaactgaa
     1801 agcccctgct ttcttgagcg tcgtgcgctt ggcgtcgtcg tagccgagca cttccttcaa
     1861 cacctcgtcg gtgtgctcgc ccaagagcgg cgagcgcttg atcttggccg gagaggcgga
     1921 cagcttgatc ggcataccga tgttgtacca cttgccgcgc tgcgggtgat ccaattcgac
     1981 atacatctcg cgccccctga cgtgctcgtc gttcgcaaga tcttcggtgg acatgaccgg
     2041 accacagggc acgttgatct cattcagaat gcccatgacc tcgcgcttgg tatgctttag
     2101 cgcgaattcg ccgatgattc cccacatgtc attctggaac ctgcggcgct cgccgatggt
     2161 ggcaaatttc ggattggtgg caagatcgga catgccgagt tccgcgccga tgagcttagc
     2221 gagcccggcc cacaccgctt cctgcacgac aatgtagata aaatcattgg tgccccccgg
     2281 cttgcagcgg atggcattac cgagctggcc gccgcctgaa tcattgccgc cacgcgggac
     2341 cgctcccatg ccctggtgcg tgcctaccga atactcgggc atcggcccgc gggtaagacg
     2401 ctggtgatcg cgccatttga cccggcacag gttcatgatg ccgtccatca ttgcgacctc
     2461 gacatactgc ccctgacccg tcttctcggc ttggcgcaaa gccgccagaa ggccgatcgc
     2521 cagatgcaga ccggtaccgg tatcgccgat ctgcgcaccg gtcacgaacg gaggaccatc
     2581 cgggacgccg gtggtgctca tcgcgccgcc catcgcctgc gccacgtttt catacgcctt
     2641 gaactccgaa tacggtccgg atgagccgaa gcccttgatc gaccccatga cgatgcgcgg
     2701 gttgatctcg tggattttct cccaactgaa accgaagcgg tcgagcacgc ccggcccgaa
     2761 gttctccatg atgatgtcgc acttctgcag caggtcgacg aacaccgcct tgccttcagc
     2821 cgttttcatg ttcacggtga tcgagcgctt gttgcagttg agcatggtga aatacaggct
     2881 gtccgcgttg ggaatgtcgc gcaactggcc gcgggtcgcg tcgccctgcg gattttcgaa
     2941 tttgattacg tccgcgccca tccacgcgag cagttgcgag caggtcggtc cggcctgcac
     3001 atgtgtcatg tcgagaatgc gaactccctt gagtgcttcc atgctagata cccctcctgt
     3061 gttcatttcc gcttgcgcgg gatcgcccgt gccctaggcg cgggtcattt gtatatcact
     3121 tgtgcatcgt ttggttcgag gtgcccgatg agcagacatc gcggtcaatc atcacgtcgc
     3181 gctagggctg atctgcgccg ctcgcaggac tcggcgcggc gcccgcacga gcgtccagcc
     3241 gggccaatcg actttcatcc gcgcgcttcg aaaccataat tcattgcgag ggcttatact
     3301 aagaaatcga accgcttgcg caccttgact gaggtcaata atttcgcttt taattcgctt
     3361 ctgaatctgg cacaaccctc acatgtcgtc catccaatcg catccccagc acaacaccat
     3421 ccggctgcag ctcaggcaga gcctggtgct caaggacatg cctctcgcgg catgggccga
     3481 gttggagccc cagctggaga tcgccgattt cggcaaaggc gacctgctgg tgcaccaggg
     3541 ccacagcgcg atggagcagt ttttcatcct cgacggcatc ctcaagcggg tggtcagcga
     3601 cccgaaaggc aaggagatga tcctgcgctt cgccgccgag aacgacatgg acacttccta
     3661 cgccgcctgg cgcctgggga cgccgatgcc ctacagcatc gtcgccgtga ccaaggtccg
     3721 cgtggccaag ctttcgatgc agcactgggt cgagtttctc gaatgtcact ccgagctcaa
     3781 agcgcgcttc gagtacgagg tcatgagcct gatgtcggaa gtgatggcgc acaccatcac
     3841 cctgcatctg ctcgacgcgc ccgggcggct gtcgcgcttc aagcgcaagc atccggaact
     3901 tctcgaccgc attccaaaaa aggaactggc ctcctatctg aacctcaccc cggaaaccct
     3961 gtcccgcctg aaaaagcgca gacagcccgg ggcaccctga caaagtccac ccagcgaaga
     4021 cagcgtcggc gcaaaaacaa caagcgccgg cttgagcctg ccaagctggc gcttctgggc
     4081 aaaacggctt cagccaggat cggcatccgc ctcggcgtcc aatctattgt gtgcgacggc
     4141 ctttgcacaa ccggccgcag gatcgtgccg atcgtgcccg cagtcagcca tcgaggccac
     4201 cctctgccaa gccacccgat caatcgcgtg gtcgggttgc tgccctttcg ctcgaagccc
     4261 gccgcttaac gccagcctac cgtatcatca cctggtagtc cccgcggtgg cggcgaatac
     4321 cggaccctta acttccccgg caacttcccc ggcgattctt tttctaatga taggccttag
     4381 gacgaaaagg gccagggccg ccgctatgag gtcgaatacg gctgcgatgt agtagggcac
     4441 cgagaaagag ccggcaaaat gtgcagccac cgcagcagca ccccatccgg ccataatgga
     4501 agccaagcct ttggccgtat acagtatgcc gtagttgccc gacgcgtttt tcgggccgaa
     4561 gacatctccc gttatcgccg agaacaaagc gtagatttcg ccccaggcaa ggaagacaaa
     4621 gggcatcagg atcatgaacg cgatcggctg tcccatgatc tgcgtcatca ggaagatgag
     4681 cacgccttca agggcaaagg caaaggccat cgtatactcg cgcccgaacc tgtctgaaac
     4741 cgagccccac atgattctgg cgaacgcatt ggtaactccc gctatggttg caacgaaggg
     4801 gacaatggcg atacccatta ccttcgcgtc gaacacgttg agggacttgg cgatctggga
     4861 catgttgccg gtagtcatca gtccgccggt acagaccatg aggaacatga agtacatgat
     4921 ataaaactct ttctgcgaca gcatctgcga cgcggaaaac tcccgccgcg tttgcgcgac
     4981 cgccttgggc tgcacccaat ctttaggcaa ccagcctttg ggggggtggt gcaaaatcag
     5041 cgccattgcg atggccacaa caccttgacc aatgccccac gcggtcatcg caccggccca
     5101 acccatggac gcaatactgc cgctgattgg gatgagcgta agcgctgagc cacctccgaa
     5161 tcccgcggcg gtcaggccgg ccgccaatcc ccttttgtcg ggaaaccatt tgacggcatt
     5221 ggcaactgtg gctatgtaga tgatgccgca gccagtaccg gcgagaacgc cgtaatagat
     5281 gtacaagtga aacacggatg ttgcgaacat tcctccgagc acccatccca gaaaaacgag
     5341 tatggcacct atgagcatga gattgcggat accgaattta tcgatgaagt atccggcaag
     5401 cggcaccggc caggtttcaa agagaataaa cagggaaaag atgagggcaa tatcggaata
     5461 gggcacggtc gggaactgtt gcttcagccc gggagtaaaa agcgtgaagg catactgaaa
     5521 gtttgatatg acgaccatcg ccacgacacc ggcagccaat tgaatccacc ggttgccgac
     5581 gatcggaccg aatgtttgtt tcgcgagatc tatctgagcg gtgctctcgt ttgacatttc
     5641 gcctcctcct ttgaactaca tttgacttgt tccatcgcga tcccttctag caacggacct
     5701 tgacattggt gtccggcaat tcgcttcttt gccttttttt tttgaagcgg caaccaaatc
     5761 aattgccgaa atccgcggct gagtgtcgac gcacgaagcc tacagcaaaa cattctgctc
     5821 gcgt
//
LOCUS       DATCGS010000024         4968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_398233, whole genome shotgun sequence.
ACCESSION   DATCGS010000024 DATCGS010000000
VERSION     DATCGS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398233"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VIL39_00440"
     CDS             <1..865
                     /locus_tag="VIL39_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5479923.1"
                     /translation="VREVRYPKLPPGQYRFQVTACNEDDVWNKEFATLSIKVLPPFWQ
                     TWWFLSLTTLCLLAMIVGSVHYVSTQRLQRQLAALRQKEALEKERARIARDIHDQVGA
                     NLTQLSLLSELVESDKDDPKEVEGHARQIEQTALETTRALDEIVWTVNPSNDTLDGLI
                     TYVCKYAQDYLALAALRYRLEVPPQLPSIPISPELRHNVFLAAKEAVNNIVKHSEATA
                     AWLRLRLEPGRFTLEIEDNGCGLSPGAADKGRNGLRNMRKRLEDVGGEFSIAPGAEGG
                     TRVRLTAPLGE"
     gene            1037..1459
                     /gene="rpsL"
                     /locus_tag="VIL39_00445"
     CDS             1037..1459
                     /gene="rpsL"
                     /locus_tag="VIL39_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5479924.1"
                     /translation="MPTINQLVRKGRRKLKHKSKSPALENSPFRRGVCIQVMTRTPKK
                     PNSAMRKVAKVRLTNGHEIIAYIPDEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DAAGVEKRRVSRSKYGVKRPKPGAKPAAAAAPAPAPAA"
     gene            1509..1982
                     /gene="rpsG"
                     /locus_tag="VIL39_00450"
     CDS             1509..1982
                     /gene="rpsG"
                     /locus_tag="VIL39_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5479925.1"
                     /translation="MSRRRRAVSRPIIKDAKFSSTLVTRLVNTVMQGGNKSVAQRIVY
                     GAFDQLIAKNPATNPLEVLQRAVDNAKPRLEVKARRMGGATYQVPLEVPSDRQFSLAL
                     RWLVDFADARKGIPMKEALAAEILEAYQGQGNAIRKRDEVHKMAQANKAFAHFRW"
     gene            2054..4246
                     /gene="fusA"
                     /locus_tag="VIL39_00455"
     CDS             2054..4246
                     /gene="fusA"
                     /locus_tag="VIL39_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5479926.1"
                     /translation="METVTETKKSSKTKGANVPQRQYPMERTRNIGIAAHIDAGKTTT
                     TERILFYTGLIHKMGDVDDGNTVTDWMEQERERGITITSAATTCYWTQKEDGSYKAFL
                     GLPHRINIIDTPGHVDFTAEVERSMRVLDGAVAVFCGVAGVQPQSETVWRQATKYKVP
                     RIAFINKMDRTGANFENALNDMRKKLGAYAFPIYLPIGKEDYFSGVVDVVNQKAIVYD
                     PTDDTGLKYQVTDIPAELKDSAKAALAELVDAVSNKDEKIAELIIENKPVSPELLKAA
                     IRRLTCKIELVPVLCGSAFKKRGVQPLVDAVVDYLPSPLDVPPAFGFVPGTETKVDVP
                     TDDNGKFCSLAFKLWTDPYVGKLVFFRLYSGRLSKGDVIYNPRTRKRDRVSRVMMIQA
                     DKRIDVETCFSGDIAALVGLRNITTGDTLCDEDFDVMLEPPTFPEPVISMAVEPKTKA
                     DREKMGEGLQRLAEEDPTFRCFTNVDTGQLIIAGMGELHLEIIRDRLFREFKVSANAG
                     APQIAYRETVTKAAEGEGKFIRQSGGKGQYGHACVTLEPNGRGKGVEIENKIVGGAIP
                     KEYIAPVIDGIEEAIKGGVLAGYPVVDVKVAIVDGTYHEVDSSELAFKMAGIFALKDA
                     AKKASPILLEPIMKVEVTTPDEYQGDLLGDVNRRRGKIVHIEAKDASTILTAEVPLAE
                     MFGYATAIRSLSKGRAAYSMEPFRFEPVPNSIVAGILDAAKGKPAART"
     gene            4348..>4968
                     /gene="dapF"
                     /locus_tag="VIL39_00460"
     CDS             4348..>4968
                     /gene="dapF"
                     /locus_tag="VIL39_00460"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5479927.1"
                     /translation="MNGAGNDFVLIDNRARKIKLSRDQVVRLCDRHRGVGADGAVLLV
                     PSVSGQADWAWEFYNSDGSPGAMCGNGARCFSRFVQKLTGLTRGFSFETEAGVITASF
                     QGDRVTVSLTRPKDLRLNEQVALSIGTQTIHSVNTGVPHAVLFVPDTDRVMVQQLGSE
                     IRHHAHFSPKGANVNFVQWLGPNHIGVRTFERGVEGETLACGTGVTA"
BASE COUNT         1034 a         1480 c         1558 g          896 t
ORIGIN      
        1 cgtccgcgag gtgcggtatc ccaagctgcc gccgggccag tatcgctttc aggtgacggc
       61 atgtaatgag gatgatgtct ggaacaagga gttcgccacg ttgtccatca aggtgttgcc
      121 tcccttctgg caaacgtggt ggttcctcag tctgacgacg ctgtgcctgc tggcgatgat
      181 cgtgggttcg gtgcactatg tttccacgca acggttgcag cgccagttgg ccgccttgcg
      241 gcagaaggaa gccctcgaga aagagcgggc gcggattgcg cgggacatcc acgaccaggt
      301 cggcgcgaac ctcacgcaac tttccctgct cagcgaactc gtcgagagcg acaaggacga
      361 tccgaaagaa gtcgagggcc acgcccggca aatcgagcag actgctctcg aaaccactcg
      421 cgccctcgac gagatcgtct ggaccgtcaa cccctcgaac gacacgctgg acggcctgat
      481 cacgtacgtc tgcaaatacg cccaggacta cctggcgctg gccgcgttgc gctaccggct
      541 ggaagtcccg ccgcaactcc ccagcatccc tatctcgcct gaactgcggc acaacgtctt
      601 cctggccgcc aaggaagccg tgaacaacat tgtcaagcac tctgaggcca ctgccgcctg
      661 gctgcgtctg cgtctggagc cgggccgctt caccctcgaa atcgaagaca acggctgcgg
      721 cctgagcccg ggcgccgccg acaaaggccg caacggcctg cgcaacatgc gcaaacgcct
      781 cgaagatgtc ggcggcgaat tctccattgc cccaggtgct gaaggcggca cgcgagttcg
      841 gctgaccgcg ccgctgggcg agtgatgggt gaggagaggt gaggagaggt gaggagaaat
      901 ctcattttga actcccgccg acattgatcg aaatagttgt tgcaactaaa gagccgtttg
      961 gtatatttcg cactccgttc gcccaaagat ctggccgcgt cagcgcggta ggcagggcac
     1021 gggaatgtaa aagcgaatgc cgacaattaa tcaattggtc cgcaagggcc gccgcaaact
     1081 gaagcataag tccaagtcgc ctgccttgga gaactcgccc tttcgtcggg gcgtctgtat
     1141 ccaggtgatg acgcgcacgc ctaagaaacc gaattcagcg atgcgcaagg tggcgaaggt
     1201 gcgcctgacc aacgggcatg aaatcattgc ctacatcccg gatgagggtc ataatttgca
     1261 ggagcactcg attgtgctgg tgcgtggcgg tcgcgtgaag gatttgcccg gcgtccgtta
     1321 ccacatcgtg cgtggcaccc tggacgccgc cggcgtggag aaacgccgcg tcagccgctc
     1381 gaagtacggc gtgaagcggc cgaagcccgg cgccaagccg gccgccgccg ccgccccggc
     1441 ccctgcgcca gccgcctgac gcgctgaaca aataacgatt taactcattt aactaattta
     1501 acgaacacat gtcgcgacgt cgtcgagcag tcagtcggcc catcatcaaa gacgcgaaat
     1561 tcagcagtac gctggttacg cgcctggtga atacggtcat gcaaggtggc aacaagagtg
     1621 tggcgcaacg gattgtctat ggcgcctttg atcaactaat tgccaagaac ccggctacca
     1681 acccgctgga agttttgcag cgggccgtgg ataatgcgaa gccgcgcctg gaagtcaaag
     1741 cgcggcggat gggcggggcc acctaccagg tgccgttgga agtgccgagc gaccgccagt
     1801 tctcgctggc gctccgctgg ctggtggatt ttgccgatgc gcgcaagggc attccgatga
     1861 aggaagccct ggcggcggag atcctggagg cctaccaggg gcagggcaac gcaattcgca
     1921 aacgcgacga ggttcacaag atggcacagg ccaacaaggc ttttgcccat ttccgctggt
     1981 aatccttttc agcttaacgc cccgagggcc gaagcgaccc ggggcgtttt tattttcaac
     2041 cccatatacc tggatggaaa cagttacaga aacaaagaaa tcgtcgaaga ccaaaggggc
     2101 gaacgtgccc cagcggcagt atccgatgga gcgcacgcgc aatatcggca tcgccgcgca
     2161 tattgacgcc ggcaagacca ccaccaccga gcgcattctc ttttacacgg ggctcatcca
     2221 caagatgggc gacgtggatg acggcaacac agtgactgac tggatggagc aggagcggga
     2281 acgcggcatt acgatcacct ccgcggcgac cacttgctac tggacccaga aggaagacgg
     2341 cagttacaag gcgtttctcg gcctgccgca ccggatcaat atcatcgaca ccccggggca
     2401 cgtggatttt accgccgaag tcgagcgctc catgcgcgtg ctggacggcg cggtggcggt
     2461 gttctgcggc gtggccggcg tgcagccgca atcggagacg gtctggcggc aggcaaccaa
     2521 gtacaaagtc ccccgcatcg ccttcatcaa caagatggat cgcaccgggg ccaacttcga
     2581 gaatgcgctc aacgacatgc gcaagaagct cggcgcctac gctttcccga tctacctgcc
     2641 cattggcaaa gaggattact tcagcggcgt cgttgatgtc gtgaaccaga aagccattgt
     2701 ctatgatccg actgacgaca ccggcctcaa ataccaggtc acggacattc cggcggaact
     2761 gaaagattcg gccaaggcgg ctttagcgga gctggttgat gccgtttcca acaaggacga
     2821 gaagattgcg gagctaatca tcgagaacaa gccggtcagc ccggagctct tgaaagcggc
     2881 catccggcgg ttgacttgca agatcgagct ggtccccgtg ctgtgcggct cggccttcaa
     2941 gaagcgcggc gtgcagccgc tcgtcgatgc cgtcgtcgat tatctgccct ccccgctcga
     3001 tgtgccgccc gccttcggct tcgtcccggg cacggagacg aaggttgatg tgcctaccga
     3061 cgacaacggg aagttctgct ccctggcgtt caagctctgg acggacccct acgttggcaa
     3121 gctggtgttc ttccgcctgt atagcggccg cctgagcaag ggcgatgtga tttacaatcc
     3181 gcgcacgcgc aagcgcgacc gcgtgagccg tgtcatgatg atccaggccg acaagcgcat
     3241 tgacgtggaa acctgttttt cgggcgatat cgccgcgctg gtgggcttga ggaacatcac
     3301 caccggtgac acgctttgcg acgaggactt cgacgtcatg ctcgagccgc cgaccttccc
     3361 ggagcccgtc atctcgatgg cggtcgagcc caagaccaag gccgaccggg agaagatggg
     3421 cgaggggctg cagcgcctgg cggaagagga cccgaccttc cgttgcttca ccaacgtgga
     3481 caccggccag ctcatcattg cgggcatggg tgagttgcac ctggagatca tccgggaccg
     3541 tctgttccgc gagttcaagg tgagcgccaa cgcgggcgcg ccgcagattg cctatcgcga
     3601 gaccgtcacc aaggccgccg aaggcgaagg caagttcatt cgccagtctg gcggtaaagg
     3661 ccaatacggc cacgcgtgcg tcacgctgga gcccaatggg cgcggcaaag gggtcgagat
     3721 cgagaacaag atcgtcggcg gcgctattcc caaggaatac atagcgccgg tgattgacgg
     3781 catcgaggag gccatcaagg gcggcgtgct ggcgggctac ccggtggtgg atgtcaaggt
     3841 ggccatcgtc gatggcacat accacgaagt tgattccagt gagctggcct tcaagatggc
     3901 cggcatattc gctctcaaag acgcggccaa gaaggccagc ccgatcctgc tcgagcccat
     3961 catgaaggtg gaggtcacca cgcccgacga ataccagggc gatttgctgg gcgacgtcaa
     4021 ccggcgccgg ggtaagatcg tccacatcga ggccaaagac gcttcgacga ttctgaccgc
     4081 cgaagtgccg ctggcggaga tgttcggtta tgcgacggcg atccgttcgc tgtcgaaagg
     4141 ccgggctgcg tattcgatgg agccgttccg cttcgagccg gtgccaaaca gcatcgtcgc
     4201 cggtatcctg gacgccgcga aaggcaagcc cgccgctcgc acctgaccca tcggctactt
     4261 gccggcggct gcgcccgagc caactttgga ctttcaactt cggactttgg actatacctt
     4321 ctgtgtatgg ttttggagtt cacaaagatg aatggcgcgg ggaatgattt tgtgctcatt
     4381 gacaaccggg cacgaaagat caagctttcc cgcgaccagg tcgtgcggct ttgcgaccgg
     4441 catcgcggcg tgggggcgga tggcgccgtg ctgctggtgc cgtcggtttc gggccaggcg
     4501 gattgggcat gggaatttta taacagcgac ggcagccccg gcgcgatgtg tggcaacggc
     4561 gcgcgctgct tctcgcgctt tgtgcaaaag ctgaccggcc tcactcgcgg gttttctttc
     4621 gagacagagg cgggcgtcat tacggccagc ttccagggcg accgggtcac ggtcagcctg
     4681 acccggccaa aagacctgcg gctcaatgag caagtggcgc tgtccatcgg cacccagacc
     4741 attcattcgg tcaatacggg cgtgccccat gcggtgctgt tcgtgcccga caccgaccgg
     4801 gtgatggtgc agcagctcgg ctccgaaatc cgccaccatg cgcacttcag tccgaaaggg
     4861 gcaaatgtga atttcgtgca gtggctgggg ccaaatcata ttggggtacg gacattcgag
     4921 cgcggggtgg agggcgaaac gctggcctgc ggcaccgggg tgacggcc
//
LOCUS       DATCGS010000025         4622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_2775, whole genome shotgun sequence.
ACCESSION   DATCGS010000025 DATCGS010000000
VERSION     DATCGS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1077)
                     /locus_tag="VIL39_00465"
     CDS             complement(355..1077)
                     /locus_tag="VIL39_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY5479928.1"
                     /translation="MTKISWSVLISTLLLAGGLSAQTFNALNAPLVPAPRTSPTNWLA
                     RHEGFVAQAKSGGIDLLFMGDSITDGWRTRGSNVWRKCYEPRHAANFGIGGDRTQHVL
                     WRIRTGELTGIKPKVTVLMIGTNNSKDDQPEPIANAIKLIVQGIRTQCPGTKVLLLAV
                     FPRNKTTDTPEQLATNRKVNEIIAKLDDGKNVRFLDINKVFLGPDGKVPKDIMPDFLH
                     PNEHGYQLWADAMEPTLAEMMK"
     gene            complement(1256..2074)
                     /locus_tag="VIL39_00470"
     CDS             complement(1256..2074)
                     /locus_tag="VIL39_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY5479929.1"
                     /translation="MELLSLMKWPLIASLILPWLLVYLGLHIVQRGVIFVDLALAQTA
                     AFGTCISLLAGYDVHDWQSYAFSLGFTFVGAVVLTFTRGRHKRVPQEALIGIVYVVAA
                     AAAILALSKSAGGHEELQRSLVGELLVVLPADVMKTFGLFVLLGAVHFAFRKKFLAIS
                     ADPEKAEAGGLNVRWWDFIFYMLFGLVVTSFVHIGGVLLVFSYLVVPAVCATYLVNSI
                     AARFAVGWGIATLASVVSLFVTARVDLPIGAAIVCALGAALVLVEIAVRCFRRA"
     gene            complement(2126..3046)
                     /locus_tag="VIL39_00475"
     CDS             complement(2126..3046)
                     /locus_tag="VIL39_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEY5479930.1"
                     /translation="MKLNIKFLLGCLLFAALCEPALAKLNVVATTADLASIAKAIGDE
                     RIDLTVLAKPTEDPHFVDAKPSLILKLNRADVVVEGGAELEIGWLPRLLDEARNTKLA
                     ASAAGHVLCSQGVALKEIPSTLDRSKGDIHAAGNPHYIVDPVNAKIVARNITEAFCAL
                     DEKSAAAYRANLQKFTEALDAKLVEWQKKLEPFKGQQVVAYHNSWLYFGDRFGLNIDL
                     FLEPKPGVPPTPRHLAEVILKMKQDKVHVILVDPYLDRRTAETVAAKTDATVVDVAQF
                     PGGVKGTEGGYIALVDYLVNSVAKALASTK"
     gene            complement(3069..4529)
                     /locus_tag="VIL39_00480"
     CDS             complement(3069..4529)
                     /locus_tag="VIL39_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479931.1"
                     /translation="MVAAALTAMGFSGAPTLRAQATNEVEQLKRQMQQLQENFERVQR
                     EQRQQIDALTRKLDDLTQQQTAEAEKKKLEQELAADLQKNQPATVAPVTPAAPSVPWS
                     PSQPITVARAGSAYMNISFDTLMDFGGSTAADPSRFLQLGDHDPISRGFSLRNAEIAL
                     DGAVDPYFKGFANIALKLDQNNETSIELEEAYLQTTSLPANLQVKAGQFYAAFGRQNS
                     QHPHQWAFVDDPLILTRAFGPDGLRNIGAQVSWLAPTPFYTEAFLGVFDPQGGTAFSF
                     RNPGEPDVFGVQRVHGRTPFDQNIRGPGDLLYVPRLASSFDLTDYQTLVVGASAAFGP
                     NDTGAGLHTQIYGLDAYWKWKPANAHAGFPFVSWQTEALYQRFEAGADPTASLPAETL
                     NDWGFYSQVLWGFKERWVAGLRGDYAGGNPGAYDPNDVFRGQRTRISPGLTFYPSEFA
                     KIRLQYNLDHGEQFGTEHSVWLQVEFLLGAHGAHKF"
BASE COUNT          898 a         1329 c         1518 g          877 t
ORIGIN      
        1 actgattttg cggccttgaa cgatttcggc ttccagcacg cggccaaact accaagcaga
       61 tttcgccgag tcccgtacaa agcgaaactg tttttgcgct aaatcccgcc gccagtgctc
      121 atctggcccc tgagtaatta cggttacagc gttaagagac ttggcactct ttgaccacgg
      181 tctcagcttt gaaacgccgg gctattcccg gtcatccctc cgcttgaggc gtatcaaaag
      241 ctagcggcat tggactctaa ggtccgcctc gctgcaaccc atccgcttca gcaacgacga
      301 actgtgccat tagcgcagcg gcggccggca ggaagtttcg catcggacgc tgcactactt
      361 catcatctct gccagcgtcg gttccatcgc gtcggcccag agctgataac cgtgttcgtt
      421 cggatgcagg aagtcgggca taatgtcttt cgggactttg ccatcggggc cgaggaacac
      481 tttgttgatg tcgaggaagc gaacgttctt gccgtcatca agcttggcga tgatttcgtt
      541 aacctttcgg ttagtggcga gttgctccgg cgtgtcagtg gttttgttgc gcgggaacac
      601 agccagcaac agcactttgg tgccggggca ctgggtgcga atcccctgca caatgagctt
      661 gatggcgttg gcgatgggtt cgggctgatc atccttgctg ttgttggtgc cgatcatgag
      721 cacggtaacc ttgggtttaa tgccggtcag ttcgccggtc ctgatgcgcc agagcacgtg
      781 ctgggtgcgg tcgccgccga tgccgaagtt ggcggcgtgg cggggctcgt agcacttgcg
      841 ccagacattg gagccgcgag tgcgccagcc atcggtgatg gaatcgccca tgaagagcag
      901 atcaatgccg ccgcttttgg cctgggcgac aaagccctcg tggcgggcga gccagttggt
      961 gggactggtg cgcggcgccg gcaccaaagg agcattcagc gcattgaagg tttgggccga
     1021 cagaccgcca gccagcaata gagttgagat gagaacggac caagatattt tcgtcatacg
     1081 aagtgccgca atctaggcaa agcagggcgc ggggcgcaag atcctttgcg gctcgccatt
     1141 gggactgatc acaaccaaca cttgacttgc agggcgcccc cctcccaaac agttggctgg
     1201 tatgtggctt tagggtggct ttagcgcgca agccgaatgg caacgggtgt cgcactcagg
     1261 ctcggcgaaa gcatctcacc gcaatctcca ccagcaccag ggccgcgccc agtgcgcaga
     1321 cgatggcggc gccgatgggg aggtccacgc gagcggtgac gaagaggctg acgacgctgg
     1381 ccagagtggc gatgccccag ccgacagcga agcgggcggc gatggagttg accaggtagg
     1441 tggcgcagac cgccggcacc acgaggtagg agaagacgag cagcacgccg ccgatgtgca
     1501 caaagctggt caccaccagg ccgaagagca tgtagaagat aaaatcccac cagcgtacat
     1561 tgagaccgcc ggcctccgcc ttttccgggt cggcggagat ggcgaggaac ttctttcgga
     1621 acgcgaagtg aacagcgccc agaaggacaa acaggccaaa ggtcttcatc acgtcagccg
     1681 gcagcacgac caggagttcg ccgacgagtg agcgttggag ttcctcgtgg cccccggcgc
     1741 tcttgctcag ggccaggatg gcagccgccg ccgcgacgac atagacgatg ccgatgaggg
     1801 cctcctgggg gacacgcttg tgacgaccac gcgtgaaggt caataccacc gcgccgacga
     1861 aggtgaagcc gagcgagaag gcgtagcttt gccagtcgtg gacgtcgtag ccggcgagca
     1921 gggagatgca ggtgccgaag gcggcggtct gggccagcgc caggtccacg aagatgacgc
     1981 cgcgctggac gatgtgcagt ccgaggtaaa ccagcagcca gggcaggatc aggctcgcaa
     2041 tgagcggcca tttcatcagg gagagaagtt ccatacgcga ttggagtggt ggagtggtgg
     2101 agtaatggag tcgtgacgtg gtggattact tagtgctggc gagagctttt gccacggagt
     2161 tgaccaggta atccaccagg gcgatgtagc cgccctcggt gcctttgacg ccgccgggga
     2221 attgcgccac atcaaccacg gtggcgtcgg tcttggcggc gacggtctcg gcggtgcgcc
     2281 ggtccaggta gggatcgacc aggatgacat ggaccttgtc ttgtttcatc ttcaagatca
     2341 cttccgccag gtgcctcggg gtcggcggga cgccgggctt gggttccagg aacaagtcaa
     2401 tgttcaggcc gaagcggtcg ccgaagtaga gccacgagtt gtggtaggcg acgacctgct
     2461 ggcctttgaa gggttcgagt ttcttctgcc actccaccag cttcgcgtcg agggcctcgg
     2521 tgaacttctg caagttggcg cggtaggcag cagcggactt ctcgtccagg gcgcagaaag
     2581 cttcggtgat attgcgggcg acgatcttgg cgttgaccgg gtccacgatg taatgaggat
     2641 tgccggcggc gtgaatgtcg cccttggagc ggtcgagggt ggaggggatc tccttcaggg
     2701 cgaccccctg ggagcaaaga acatggcccg cggcactggc ggccagcttg gtgttgcgcg
     2761 cctcgtccag caagcgcggc agccaaccta tctccagttc cgcgccgccc tcaacgacca
     2821 cgtcggcccg gttcagcttc aggatcaagc tgggcttggc atccacgaaa tgaggatcct
     2881 cagtcggctt ggcgaggacg gtgaggtcaa tgcgctcatc gccgatcgct ttggcgatgg
     2941 aggccaggtc tgccgtggtg gcaacgacgt tgagtttggc caaagccggc tcgcataacg
     3001 cggcgaatag cagacagccc agcaggaact taatgttcag tttcataatt gtattggtgg
     3061 attgttaatc agaacttgtg cgccccatgc gcgcccagca aaaactcgac ctgcagccag
     3121 accgaatgct ccgtgccgaa ctgctctccg tgatcgagat tatactggag ccgaatcttg
     3181 gcgaactcac tgggataaaa ggtcaggccc ggcgaaatgc gggtgcgctg gccgcggaaa
     3241 acgtcgttgg gatcgtaagc gccggggttg ccgccggcgt aatcgccgcg caagccggcg
     3301 acccagcgct ctttgaaccc ccagagcact tgtgagtaga agccccagtc attgagcgtt
     3361 tccgccggca acgaagccgt cgggtctgcg cccgcctcga atcgctgata cagcgcttcg
     3421 gtttgccagg agacgaaagg aaacccggcg tgggcgttgg ccggcttcca tttccagtaa
     3481 gcgtccaggc cgtagatttg ggtgtgcaac cccgcaccgg tgtcgttcgg accaaaggcc
     3541 gccgacgcgc cgacgaccag agtctggtag tccgtcagat cgaacgagga agccaggcgc
     3601 ggcacataca acaaatcgcc ggggccgcgt atattctggt cgaacggcgt gcggccatgg
     3661 acgcgctgca ctccgaatac atccggctcg cccgggttgc ggaaactgaa agcagtcccc
     3721 ccctgcggat cgaatacgcc cagaaatgcc tcggtgtaaa acggcgtcgg cgccagccac
     3781 gacacctggg cgccgatgtt ccgcaatccg tccgggccga aggcgcgggt gagaatcagc
     3841 ggatcatcaa caaacgccca ttggtgcgga tgctgggagt tctgccggcc gaaagcggca
     3901 tagaactggc cggccttgac ctgcaggttg gcgggcaggg aggtggtttg caggtaagcc
     3961 tcctccagtt caatgctggt ctcgttgttc tggtcgagct tcagcgcgat attggcgaac
     4021 cctttgaaat acgggtccac ggcgccatcc agcgcgattt ctgcgttgcg cagcgagaag
     4081 ccgcggctga tggggtcgtg atcgccgagt tgcaagaacc gggaagggtc ggcagccgtg
     4141 gacccgccaa agtccatcaa cgtgtcgaag ctgatgttca tataggccga accggcacgc
     4201 gcgacggtga tgggctgcga gggcgaccat gggacggacg gcgcggcggg ggtgaccggg
     4261 gcgacggtgg caggctggtt cttctggagg tctgccgcca gttcctgctc gagcttcttc
     4321 ttttccgcct cggcggtctg ctgttgggtc aggtcgtcca gcttcctggt gagcgcgtct
     4381 atttgctggc gctgctcgcg ctggacccgt tcgaagttct cctgcaactg ctgcatttgc
     4441 cgcttgagct gctccacttc attcgtggcc tgggcccgca gcgtcggcgc gccgctaaag
     4501 cccatcgcgg tcagcgcggc ggcaaccatg atagggaacc atgcacgcct tggacgtgcc
     4561 tcgcggacag gcggagaacg acggcctcgt ctccctgccc ctgaaccgcc atatagtggg
     4621 gc
//
LOCUS       DATCGS010000026         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_646143, whole genome shotgun sequence.
ACCESSION   DATCGS010000026 DATCGS010000000
VERSION     DATCGS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646143"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VIL39_00485"
     CDS             complement(<1..1258)
                     /locus_tag="VIL39_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5479932.1"
                     /translation="MSNDNGTPLTRRRFLTRGAMVAGAVALPCYIPATALGRNGAVAP
                     SERIVMGGIGMGGRGSYDLGAMLNERDVQWVAVCDVLKNRREAAKNTVDRKYGTKDCA
                     AYADLRQLLAERKDVDAVLIATGDRWHALASVMAMRAGKDVYCEKPACLTMAQSQTVV
                     ETARRYGRVYETGAQRLSEPNHVFAIEMARTGRLGQVHTVYADCRWRDGLRHDWLPAQ
                     PEPPKDELDWDVWLGPSPWRPYNTGYVNGGGWYHYYDLATDVAMWGAHTVTQALAGLD
                     MANVSFIEFEYAGPDVTMQTRLSNGVKLVLFRTTGSVWEPCEFWHGACGERFEGPEGW
                     VAAADGYSGPDVSSPALLREYKNVLADYTARTQRPLNHVRDFLDCIRSRRLTVANPEV
                     MCRSINICLAADICEQLKRSLKFDVRQ"
     gene            complement(1377..3056)
                     /locus_tag="VIL39_00490"
     CDS             complement(1377..3056)
                     /locus_tag="VIL39_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5479933.1"
                     /translation="MKTLAAIIAVSATMLTTSAPANASSLASDLVIVNASLHTMDDAH
                     PTADAVAILGNRIAAIGSTAEIRALAGPGTRVIDAAGKVVFPGFNDAHTHFLMGGFAL
                     ANVDLRSANSPEELARRLGEYAKKLPKGRWILGGEWDHEKWPGAPLPTKEMIDAATPD
                     YPVFVSRLDGHMALANSLALKLAGVTKETKDPPGGLIVHDAKTGEPTGVLKDGAESLV
                     ERVVPDKSFEEKHIAAKAATDHAAQVGVTSLTDMSADGDVGLYQYMLERGELKTRIYA
                     ASSIVSWEVMAKTGVRAAFGSDRLRIGGLKGFADGSLGSTTAFFFKPYNDAPDTRGLL
                     FDQMLPEGSMLKRVEGADKLGLQVMIHAIGDEANLRILDIYRQAAEKNGPRDRRFRIE
                     HAQHLRPSEILRFGQQKVIASMQPYHAADDGCWCDKRIGPERSKDTYAFRSLLDTGAV
                     LAFGSDWTVAPLNPMTGIKAAVTRQTLDGKHPNGWTPEQKLTVDQAVRAYTVGSAYAE
                     FAEKVKGTIAPGKLADLVMLDRNIYQINPAEIDQTRVVLTIMDGQVVWQAK"
BASE COUNT          626 a         1087 c         1024 g          666 t
ORIGIN      
        1 cctggcgcac atcgaacttc aggcttcgct ttaattgctc gcaaatgtcg gccgccaggc
       61 agatgtttat ggaccggcac atgacttcag gattcgctac cgtgagtcgg cgcgaccgaa
      121 tgcagtccag gaaatcccgc acgtggttca acggccgttg ggtccgggcc gtgtagtccg
      181 ccagcacgtt cttgtattcg cgcaacaggg ccggcgatga aacatcgggt cctgaataac
      241 cgtcggcggc cgccacccat ccttccggac cctcgaaccg ctcaccacaa gcgccatgcc
      301 agaactcgca cggttcccaa accgagccag tggttcggaa cagaaccaat ttcaccccgt
      361 tcgacaagcg cgtctgcatc gtgacatccg gccccgcata ctcgaactcg ataaaggaca
      421 cgtttgccat atcgagtccg gccagggcct gggtaacggt gtgcgcgccc cacatggcga
      481 catccgtagc caaatcgtaa tagtgatacc agcctccgcc gttcacatag ccagtgttgt
      541 aaggccgcca gggagaggga ccgagccaga catcccagtc cagctcatcc ttggggggtt
      601 ccggttgcgc cggcaaccag tcgtggcgca acccgtcgcg ccaacgacaa tcagcataga
      661 cggtgtgcac ctggcccaat cggccggtcc gggccatttc gatggcaaac acgtgattcg
      721 gctcgctgag ccgctgggcg ccggtttcat aaacccggcc atagcgccgg gccgtctcca
      781 ccactgtctg gctctgcgcc atggtgagac aggccggctt ctcacaatag acatccttgc
      841 ccgcccgcat cgccatgacc gaggcgagcg cgtgccagcg gtcgccggtg gcaatcaaca
      901 ccgcgtccac atccttccgc tcggccagaa gctggcgcag gtccgcatac gcagcacaat
      961 ctttggtgcc gtatttgcgg tccaccgtgt tcttggccgc ttcgcgccgg tttttcagga
     1021 catcacaaac agccacccac tgcacatccc gctcgttcag catggcgccc aaatcataag
     1081 aaccgcggcc gcccatcccg atgccgccca tcacaatgcg ttcgctgggc gcaacggctc
     1141 cgttacggcc cagcgcagtc gccggaatat agcagggcaa cgccaccgca cctgcgacca
     1201 tcgccccgcg tgtcaagaac cggcgtcgtg tgagtggagt tccgttgtca ttgctcataa
     1261 gcagttccat ggttaccatt gattcaccgg tacttgcaac ggcatttgca tcggcatttg
     1321 catcgagacg ggggtcagaa caaaggttct ccacaatggg ccctgctgga gcgaagttac
     1381 ttcgcctgcc agacgacctg gccgtccatg atcgtcagga cgacacgggt ctggtcaatc
     1441 tccgccggat tgatctgata gatgttccgg tcgagcatga cgaggtccgc cagcttgccg
     1501 ggcgcgattg tgcctttgac cttttcggcg aactcggcat aggctgagcc gacggtgtaa
     1561 gcgcgcacgg cttgatccac cgtgagcttc tgctcgggcg tccagccgtt gggatgcttg
     1621 ccgtcgagtg tctggcgcgt cacggccgcc ttgatgcctg tcatcgggtt gagcggggcg
     1681 acagtccagt ccgagccgaa ggccagcacc gccccggtat cgagcagtga gcggaaggca
     1741 taggtatcct tcgagcgttc cgggccaatg cgtttgtcgc accagcagcc gtcgtcagcg
     1801 gcgtgatagg gctgcatcga ggcaatcacc ttttgctggc cgaaccggag gatttcgctg
     1861 gggcgcagat gctgggcgtg ttcaatgcgg aagcggcggt cacgcggccc gttcttctcg
     1921 gcggcctgcc ggtagatgtc caggatgcgc aggtttgcct cgtccccgat ggcatgaatc
     1981 atgacctgca agccgagttt gtccgcgcct tcgacgcgtt tgagcatgct gccttcgggc
     2041 aacatctggt cgaacagcaa cccgcgtgtg tcgggcgcat cattataggg cttgaagaag
     2101 aaggcggtgg ttgaaccgag gctgccgtcg gcgaatcctt tcagcccgcc gatgcgcaaa
     2161 cggtcgctgc caaacgccgc gcgcacgccg gtcttggcca tcacctccca tgacacaatc
     2221 gaggaggcgg cgtaaatgcg cgtcttcaat tccccgcgtt ccagcatgta ctggtaaaga
     2281 ccgacatcgc catcggcgga catatcggtc aggctcgtca cacccacctg ggcggcgtgg
     2341 tccgtggccg ccttggccgc aatatgcttt tcttcaaagc tcttgtccgg caccacccgc
     2401 tccaccaggc tctcggcgcc atccttcaaa acgccggtcg gctcgcccgt tttagcgtcg
     2461 tgcacgatga ggccgccggg cgggtctttg gtttccttcg ttacccctgc cagcttgaga
     2521 gcgaggctgt tcgccagcgc catgtggcca tcgagccgac tcacgaacac cgggtaatcc
     2581 ggggtggcgg catctatcat ttccttcgtc ggcagcggcg cgcccggcca cttttcgtgg
     2641 tcccattcgc cgccgagaat ccagcggccc tttggcagct tcttcgcata ctcgcccagc
     2701 cgccgggcca gttcctcggg agaattcgcg ctgcgcaaat caacattggc tagggcaaac
     2761 ccgcccatga ggaaatgagt gtgggcgtcg ttgaaacccg ggaagaccac cttccctgcg
     2821 gcgtcaatga cccgcgtccc gggcccggcc agcgcgcgaa tctcggctgt ggaaccaatg
     2881 gcagcgatgc ggttccccag gatcgccacg gcgtcggctg tgggatgggc atcatccatc
     2941 gtgtgaaggg aggcgttaac gatgacgaga tcggaagcga gcgacgaggc attggccgga
     3001 gcagaagtgg tcagcatagt ggcagaaact gcgataatgg ctgcgagtgt tttcattgga
     3061 cgatggttta gctcatccga atcgcagacg gatacctcag ccctgggcgt ttgtcacgcc
     3121 cgataagccg gaccgccagc cgccgcagcg ccaattcatg aaggtccttt ggcgcagagg
     3181 aggtgcttca ttctgggctg ctggctgcgg ggtgattcgt gctcaggggc tagcgcccca
     3241 tgggctgagg gttctgaaag cagcaagagc cccgcagggt ggagttccaa gcgcgtcagg
     3301 atgggtcggc cccctggatg ccctccaggc gctcgacacc acccccggcc cggtgtcgct
     3361 agggtgtctc caccgtattg ctacggtatt gctacggtat ctc
//
LOCUS       DATCGS010000027         4130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_2993, whole genome shotgun sequence.
ACCESSION   DATCGS010000027 DATCGS010000000
VERSION     DATCGS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2731)
                     /gene="aceE"
                     /locus_tag="VIL39_00495"
     CDS             complement(65..2731)
                     /gene="aceE"
                     /locus_tag="VIL39_00495"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY5479934.1"
                     /translation="MLPETEQTEEEKWLESLETVLEYALKDKGPQKTARFLDGLTDRL
                     RGRGIEVPRVVSTPYINTIPADKQAPFPGDWEMERRIKSYVRWNAMAMVVNANREHSG
                     LGGHISTYASSATLCEVGFNHFFRGRTDDFIGDMVFFQGHATPGVYARAFLEGRLDQR
                     HLQNFRQELAEGGGLSSYPHPYLMPDFWQFPTVSMGLGPLVSIYQARFNRYLQARGFI
                     SGEEPKVWAFIGDGESDEPETLGSLTLASRENLDNLIWVVNCNLQRLDGPVRGNGKII
                     QELEAAFQGAGWNVIKVIWGSDWDKLLAADKTGLLLKRMEEAVDGDYQKYSVEPGSYT
                     RRHFFGKYPELLELVNHLTDEQIHKLLRGGHDSRKVYAAYKAAMEHKGQPTVILAKTV
                     KGYGLGEAGEGRNITHQQKKLNEKELREFRARFGIPVSDEEIAQTPFFRPAPDSRETK
                     YLLGLRKKLGGFLPRRIVNAKPLEVPKLEAFGDLLKGAGRSELSTTMGFVRLLGLLIR
                     NQTIGRQIVPIIPDEARTFGLDALFREIGIYSSKGQLYEPVDSKSLLYYNETKDGQIL
                     EEGITEAGSMASFVAAGTSYATHGRYMVPFYVYYSMFGFQRIGDLMWLAGDIRAKGFL
                     LGATSGRTTLNGEGLQHQDGHSLILSSTIPTLATYDPAFTYEVVVIIADGLRRMYVEG
                     ENLFYYLTLYNENYEMPPMPEGVAEGILKGLYKFNAGPGKKTLKAHIFGSGPIIREAL
                     RAQQILAEQFDVSADVWSATSYKLMRNDALRAKRWNMLHPLDPPKKSYIETVLAEEQG
                     VFVAVSDNLKIVPDQIAPWVPGGLTTLGTDGFGRSDTRERLRRFFEVDAESVVVATLH
                     ALAAKGQLDKRVIAKAIKDLDVDPEKAYPELV"
     gene            2893..3612
                     /locus_tag="VIL39_00500"
     CDS             2893..3612
                     /locus_tag="VIL39_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEY5479935.1"
                     /translation="MKLCDLTLPTPAENLACDEVLLDLCEAGDSDEILRLWALPQYFV
                     VLGYANKAATEVNLPLCQSLAIPVLRRCTGGGTVLQGAGVLNYSLILRTDASGPCHSL
                     PATNRFILERHRDALAKLTTAPVEWRGQSDLAIGGLKFAGNAQRRCRRFLLFHGSFLL
                     HLDLGLLEKILPLPSRQPDYRINRSHSDFLINLNVPDHSLKAALSEAWNATAPLDPIP
                     LDQCARLAREKYALDEWNFKF"
     gene            3830..>4130
                     /locus_tag="VIL39_00505"
     CDS             3830..>4130
                     /locus_tag="VIL39_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5479936.1"
                     /translation="MMKDVLPAMGAILAAVTILLEPQPAVAAAPPQPNILVILVDDMG
                     FSDIGCYGSEIPTPNLDRLASNGLRFTQFYNTGRCCPTRASVLTGLYPHQAGVGHM"
BASE COUNT          766 a         1388 c         1133 g          843 t
ORIGIN      
        1 cgctgcaatc tgccccaaac ccgtcgaagt tccagcacta ttacgatttg ggcagaacat
       61 agctttagac gagctccgga taggctttct ccgggtccac gtccaggtct ttgattgcct
      121 ttgcaatcac acgcttatcc aactggccct tcgcggctag cgcatgcagg gtggcgacga
      181 cgaccgattc ggcgtccacc tcgaagaagc ggcgcagccg ctcgcgcgtg tcgctccggc
      241 caaatccgtc ggtgccgagg gtcgtcaaac cacccgggac ccagggcgcg atctggtcag
      301 gaacaatctt caggttgtcg gagacggcaa cgaagacccc ctgctcttcc gccagcaccg
      361 tctcgatgta agacttcttc ggcgggtcca gcggatgcag catgttccag cgtttcgccc
      421 gcagcgcgtc gttgcgcatg agcttgtaac tggtcgcgct ccagacatcg gccgacacgt
      481 cgaattgctc ggccaggatt tgctgcgcgc gcagggcctc gcggatgatc ggcccgctgc
      541 cgaagatatg cgccttgagc gttttcttcc ccggaccagc gttgaacttg tagagccctt
      601 tgagaatccc ctccgccacg ccttcgggca tcggcggcat ctcatagttc tcgttgtaga
      661 gcgtcaggta atagaagagg ttttcgcctt cgacatacat gcgccgcaga ccgtcggcga
      721 taatcaccac cacctcgtac gtgaacgcgg gatcgtaggt cgccagcgtt ggaatcgtgc
      781 tcgacaggat caggctgtgc ccgtcctggt gctgcaagcc ctcgccgttg agggtagtgc
      841 ggcccgaggt cgccccaagc aggaagccct tggcacgaat atcgcccgcg agccacatca
      901 gatccccgat gcgctggaaa ccgaacatcg agtaatacac gtaaaagggc accatgtagc
      961 ggccgtgcgt cgcgtaggac gtgcccgccg ccacaaacga cgccatcgaa cccgcctcgg
     1021 tgataccctc ttcgagaatc tgtccgtctt tcgtctcgtt atagtagagc agcgacttgc
     1081 tatccaccgg ctcgtagagc tggcccttgg acgaataaat accgatctcg cggaacaacg
     1141 catccaggcc gaacgtgcgc gcctcgtccg gtatgatcgg cacgatctgc cggccgatgg
     1201 tctggttgcg gatgagcagc cccagcaggc gcacgaaccc catcgtcgtg gacagctctg
     1261 aacgccccgc ccctttaagc aggtcgccga acgcctccag cttgggcacc tccagtggtt
     1321 tggcgttcac gatgcgccgc ggcaggaacc cgccgagttt cttgcgcaag cccagcagat
     1381 acttggtctc gcggctgtcc ggtgccggac ggaagaacgg cgtctgcgcg atttcctcgt
     1441 cgctcaccgg gatgccgaac cgcgcccgga actcgcgcag ctccttttca ttgagcttct
     1501 tctgctggtg cgtgatgttg cggccttcgc ccgcttcgcc cagcccatag cctttgaccg
     1561 tcttggccag gatcaccgtc ggctgcccct tatgctccat ggcagctttg taggccgcat
     1621 agaccttgcg cgaatcgtgg ccgccgcgca gcagcttgtg tatctgctcg tcagtcaggt
     1681 gattcaccaa ctccagcagc tcgggatact tgccgaagaa atgccgccgc gtgtagctgc
     1741 ccggctcgac cgaatacttc tggtaatcgc catccaccgc ctcttccatc cgcttgagca
     1801 gcagaccggt tttgtcagcg gccagcagct tgtcccaatc cgagccccaa atcaccttga
     1861 tgacattcca gcccgcgccc tggaacgccg cttccagctc ctggataatc ttcccgttgc
     1921 cgcgcaccgg accgtccagt cgctgaaggt tgcagttcac cacccagatc aggttgtcga
     1981 ggttctcgcg cgaagccagc gtcaaggacc ccagcgtctc cggctcatcc gattccccgt
     2041 cgccgatgaa cgcccacacc ttcggctcct cgccactgat gaaaccacgc gcctgcaggt
     2101 agcggttaaa ccgcgcctgg taaatggaca ccaagggtcc cagtcccatt gataccgtag
     2161 ggaactgcca gaagtcgggc atcaggtagg ggtgcggata agaggacagc ccgccgcctt
     2221 cggccagttc ctggcggaag ttctgcaagt gccgctggtc cagccgtccc tccaggaatg
     2281 ccctcgcgta aacccccggc gttgcgtgcc cctggaagaa caccatgtca ccgatgaaat
     2341 cgtcagtccg cccccggaag aaatggttga acccgacctc gcacaacgtg gcggacgagg
     2401 cataggtcga gatatgcccg cccaaaccgc tatgctcgcg gttggcgtta accaccatcg
     2461 ccattgcgtt ccagcggacg tagctcttga tgcgccgttc catttcccaa tccccgggga
     2521 acggcgcttg tttatcggcg ggaatggtat tgatgtaggg cgtgctgacc acacgcggca
     2581 cttcgatacc gcgcccgcgc agccggtcgg taaggccatc caggaagcgc gccgtcttct
     2641 gcggcccttt gtccttcaga gcatactcga gcactgtttc cagcgactcc agccatttct
     2701 cttcctcagt ctgctccgtc tccggcagca cctgctgctc gagcggtgaa tccttttgtt
     2761 cttttcttgg cagtttcact ttgtcaatcg gaatccaatt acttaacctc tcactggcga
     2821 cgcgtcaaat gcctccaggc gcaccccatt agatcggatg accggttaaa tgtcagcgaa
     2881 atactatagc acatgaagct ctgcgacctc acgctgccga cgcccgcgga gaacctcgcc
     2941 tgcgacgagg tgctgctcga cctctgtgaa gccggcgaca gcgacgaaat cttgcgcctc
     3001 tgggcgctgc cgcagtactt cgtcgttctg ggttacgcca acaaggcggc gaccgaagtc
     3061 aatctccccc tttgccagtc gctcgccatt cccgtgctgc gccggtgcac cgggggtgga
     3121 accgttctgc aaggggctgg tgtcctcaac tactccctga tcctgcgcac agacgcctcc
     3181 gggccatgcc acagcctccc cgcgaccaac aggttcattc tggagcgcca ccgcgacgcc
     3241 ctggccaagc tcacgacggc gcccgtggaa tggcggggcc agagcgacct cgctattggc
     3301 ggcctcaagt ttgccggcaa tgcccaacgc cgctgccggc gtttcctcct ctttcatggc
     3361 tcttttctgc tgcacctcga cctcgggctg ctcgaaaaga tcctgcccct gccctcccgc
     3421 cagccggatt atcgcatcaa ccgctcacac tccgattttc tgatcaacct caacgtccca
     3481 gaccacagcc tcaaagccgc cctcagcgaa gcctggaacg ccactgcgcc gctagaccca
     3541 atccccttag atcaatgcgc ccgcctcgcc cgcgagaaat acgcgctgga tgagtggaac
     3601 tttaagttct gagagcctgt tgaaaaagat cattgagacc cgctgcggca ggtttggagt
     3661 cccgccttta ggcggtttcc gcgccgcgcc gcctaaaggc gggactccaa accgaatttt
     3721 caaacaggct cttagcagat taccgcgccg cggaaagatg gctggaggaa ttgtctggtg
     3781 acggtaacca tttgcccctg gccgccgtcc tctgctacaa tctatgttca tgatgaagga
     3841 tgtcctgccc gcgatgggcg ccatattggc tgccgtaacg attctgttgg agcctcagcc
     3901 tgcggttgcg gcagcgccgc cgcagcccaa cattctggtg attctggtgg atgacatggg
     3961 cttttccgac atcggctgct atggcagcga aatccccaca cccaacctcg accggctcgc
     4021 ctccaacggt ctccgcttca cccagttcta taataccggc cgctgctgcc ccacgcgcgc
     4081 ttcggtgctc acggggctgt atccgcatca ggcaggcgtg ggccacatga
//
LOCUS       DATCGS010000028         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_52482, whole genome shotgun sequence.
ACCESSION   DATCGS010000028 DATCGS010000000
VERSION     DATCGS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52482"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VIL39_00510"
     CDS             <1..1169
                     /locus_tag="VIL39_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5479937.1"
                     /translation="PESKLRETWQAVQAQAGPFQKQLGARATKVTGYDVVLVTCQFER
                     TALDTKVVFDAKGQVAGLFFVPSHAAAEASGPPPYARTNAFREKAFTVGRGEWRLPGT
                     LTLPSAGGGPWPAVVLVHGSGPNDRDETIGANKPFRDLAWGLATKGVAVLRYEKRTKE
                     HAGKFKAKGLGQLTVQEETIDDALSAVAQLRATEGLDPKRVFVLGHSLGGMVAPRIGQ
                     AEPQIAGLIMLAGCLSRPLEDVMVEQARYVLSLKAKPSAEEEAWLAEVESTAAKVKKL
                     TAADVSSSTLLMGAPPAYWLDLRQHDPLAAAKGLKQPLLILQGGRDYQATPVAFGRWK
                     EALGAQPKVTFKLYPKLNHLFIAGEGKSTPDEYAQPGHVEETVVADIAECIRGR"
     gene            1297..2283
                     /locus_tag="VIL39_00515"
     CDS             1297..2283
                     /locus_tag="VIL39_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5479938.1"
                     /translation="MAQVVLVKIAAMFLVIVAGWLARRRGFLAAEFTAMLSRLVVDVA
                     FPALVFTQMLRTVNADALRAEWFSPLLSVLIVFVAYFVGLVVSPLFSGPAQRNTFIFL
                     ITVPNWVFLPLPIAEALYGAAGVRTVLLCNAGAQLVLWSFGIWILHGSIRQALRNLLT
                     NTGLWATALGIVMALLFPAARELETINAASASLGGVMGGALVQALAMVGSLTIPLSLL
                     TIGAQLGELTIAVHRFPRPLWGVILARLIFAPLVTLALGWAAIRAGVQIPDTTRMVCY
                     LIATMPVAISCSVMTERYGGDATLAAEGTFYSTLFSLLTVPAMFWLIQQFGL"
     gene            complement(2305..>3772)
                     /locus_tag="VIL39_00520"
     CDS             complement(2305..>3772)
                     /locus_tag="VIL39_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479939.1"
                     /translation="GTHRFSTLFTAHDVRRYLASEEGLNQAMDWCKQTAITKVYIESY
                     RDGYTAEKAVLENARNRFKAAGFVVSGCVTPTKVGKPSTGWKDTISCYTDPATQAKVK
                     EIFTYAAGMFDEIMIDDFWFTDCACPECESARKARTVTVGDQKFPVPGEGWDEYRCEL
                     MVQLSRSHVLAASRQANPKAKLIIKYPQWYENFHVRGYEVVRETADFDRIWVGTETRD
                     YNDKQWGGTVQYESYFIMRWLGGIGGAKCGGGWYDWLGTTEKTYLEQARMTILGGARE
                     SMLFCYGGLQGTTGPKNVEALRLQIPRLLATAREVAKRKPIGLAAYRPPNSDPGKEPR
                     VYDFVGMMGLPLVPCHEFPTNAQAAFFPVHCLKDKDFPAKLAAFIAAGKPVLITDGLK
                     IRLEGRVKLDASNVAILDVKGDPKSLLNLSQETLDPLRAPLLRPFKSSFKAPNKVALQ
                     LYEDGSWVICNFNDDVAPVELNRTRYQVADRGWLDAWK"
BASE COUNT          648 a         1144 c         1235 g          745 t
ORIGIN      
        1 tgcccgaatc caagctgcgg gaaacctggc aagccgtcca ggcgcaagcc ggtcctttcc
       61 agaaacagct tggggcccgg gcgaccaagg tgaccgggta tgacgtggtg ctggtgactt
      121 gccagttcga gcggacggcg ctggatacga aagtggtgtt tgacgccaaa gggcaggtcg
      181 ccggcctgtt ctttgtcccg agccatgccg ctgccgaggc cagcggtccg ccgccctacg
      241 cccggaccaa cgcatttcgc gaaaaggcgt tcaccgtcgg tcgcggcgag tggcggttgc
      301 cgggcacgct gacattgccg agcgctggcg gcggcccttg gccggcggtg gtgctcgtac
      361 acggctccgg gccgaatgat cgtgatgaga ctatcggggc caacaaaccg tttcgcgacc
      421 tggcctgggg attggccacc aagggcgtcg ccgttctgcg ctatgaaaaa cggaccaagg
      481 aacacgccgg caagttcaag gcgaagggcc ttggccaact cacggtgcag gaagaaacca
      541 ttgatgatgc cctcagcgcc gtcgcccagt tgcgcgccac ggagggtctg gatccgaaac
      601 gtgtttttgt cctgggccat agtttgggcg gcatggtggc gcccagaatt ggccaggccg
      661 agccgcaaat tgccgggctg atcatgctgg cgggctgctt gtcgcgaccg ctggaggatg
      721 tgatggtcga gcaggcgcgc tatgtgcttt cgctgaaggc aaaaccgtcc gccgaggaag
      781 aggcgtggct ggccgaggtt gagtcaactg cggccaaggt gaagaagctg accgcggcgg
      841 atgtttcgtc ttcgacgcta ttgatggggg cgccgccggc ctactggctg gacttgcgcc
      901 agcacgaccc gctggccgcc gccaaaggac taaagcagcc actgctcatc ctgcaaggcg
      961 ggcgggatta ccaggccacc ccggtggctt tcggtcgctg gaaagaagcg ctcggcgctc
     1021 agcccaaggt cacattcaag ctgtatccca agctcaatca cctgttcatc gccggggaag
     1081 gcaagagcac gccggacgag tatgcccaac cggggcacgt cgaggaaacg gtggtggcgg
     1141 acattgcaga gtgcattcgg gggcgttaag acgttgcaac gcgggcacca gattggttga
     1201 gggagggaac ggaaacgctg aaacgctgaa agctagaacc ggaaccaaaa ctttagcctt
     1261 tggccttcaa tcgtcagcct ttagcctctc cgccccatgg ctcaggtcgt tttagtcaaa
     1321 atcgccgcga tgtttctcgt gatcgtcgcc ggctggctgg cccggcggcg cggatttctc
     1381 gcggcggagt tcaccgccat gctcagccgc ctggtcgtgg acgtcgcctt ccccgcgctc
     1441 gtgttcacgc aaatgctgcg gacagtcaac gcggatgccc tgcgcgccga gtggttcagc
     1501 ccgctgctgt ccgtgctgat cgtcttcgtg gcgtatttcg tggggcttgt ggtatcgccg
     1561 ctgttcagcg gtccggccca gcgcaacacc ttcatcttcc tcatcaccgt tcccaactgg
     1621 gtgttcctgc cactgccgat cgctgaggcg ctgtacggcg ccgctggggt gcggaccgtg
     1681 ctgctctgca acgccggcgc gcaactggtg ctgtggtcgt tcggcatctg gattctgcac
     1741 ggcagcattc gccaggcgct ccggaatctg ctgacgaaca ccggcctgtg ggccacagcg
     1801 ctgggcatcg tgatggcgct gctgttcccc gccgcgcgcg agctggaaac catcaatgcc
     1861 gcgagcgcat cgctcggcgg tgtgatgggc ggcgcgctgg tgcaggcgct ggccatggtc
     1921 gggagcctga ccattccgct gtcgctgctg acgatcggcg cgcaactggg cgagctgacc
     1981 attgcggtgc accggttccc gcggccgttg tggggcgtca tcctcgcgcg gctgatcttc
     2041 gcgccgctgg tgacgctggc actgggctgg gcagcgatcc gggcgggcgt gcagattccg
     2101 gataccacgc ggatggtgtg ttacctcatc gcgacgatgc cggtggcgat cagttgcagt
     2161 gtgatgaccg agcgatacgg cggcgacgcg acgctggcgg ccgaggggac tttctacagc
     2221 accctcttca gcctgctgac cgtgcccgcg atgttctggt tgatccagca gttcgggctg
     2281 tgatggaaag gctaaactaa cccttcattt ccacgcatcc aaccaccccc ggtcggccac
     2341 ctgataacgg gtgcggttta attccacggg cgccacgtca tcgttgaaat tgcagatcac
     2401 ccagctcccg tcctcgtaga gttgcagggc aaccttattg ggcgctttga aactggactt
     2461 gaatgggcgc agcaatggcg cgcgcaacgg gtcgagggtt tcctgggaca agttgagcaa
     2521 cgacttggga tcgcctttca catccaggat agcgacattc gacgcgtcca gctttaccct
     2581 gccttcgagg cggattttca acccatcggt gatcagcacc ggtttgcctg ccgcgatgaa
     2641 ggcggcgagc ttggccggga aatccttgtc cttaaggcaa tgcacgggga agaacgcggc
     2701 ctgggcgttg gtggggaatt catggcacgg cacgagcggc aggcccatca tgccgacgaa
     2761 gtcgtacacg cgtggttcct tgccgggatc gctgttgggc ggccggtagg cggccaggcc
     2821 gatgggcttg cgtttggcga cctcgcgcgc ggtggcgagc agccgcggga tttgcagccg
     2881 cagggcctcg acattcttcg ggccggtcgt gccctggagt cctccgtaac aaaacagcat
     2941 gctttcgcgc gccccgccca agatggtcat gcgggcctgc tccaggtagg ttttttcggt
     3001 ggtgcccagc caatcgtacc agccaccgcc gcatttggcg ccgccgatgc cacccagcca
     3061 gcgcatgatg aaataggact cgtattgcac cgtgccgccc cactgcttgt cgttgtagtc
     3121 gcgcgtctcc gtgcccaccc aaatccgatc aaagtccgcc gtttcgcgca ccacctcata
     3181 accgcgcacg tggaagttct cgtaccattg gggatacttg atgatgagct tcgccttggg
     3241 attggcctgc cgggaggccg caagcacgtg gctgcgcgag agttgcacca tcaactcgca
     3301 gcggtattcg tcccagcctt cgccggggac ggggaatttc tggtcgccca cggtgacggt
     3361 gcgcgccttg cgggccgact cgcactcggg gcaggcgcaa tccgtgaacc aaaagtcatc
     3421 tatcatgatc tcatcgaaca tcccggcggc gtaggtgaag atctctttga ccttcgcctg
     3481 cgtggcgggg tcggtgtagc aggagatggt gtccttccat cccgtcgagg gcttgccgac
     3541 tttggtgggg gtgacgcagc cggagaccac aaagcccgcc gccttgaatc tgttgcgggc
     3601 gttttccagc acggcctttt ccgccgtgta tccgtcgcgg tatgactcaa tatatacctt
     3661 ggtgatggcg gtttgcttgc accaatccat ggcctggttc agcccttcct cactggcgag
     3721 gtagcgacgc acatcatggg cagtgaacag ggtggaaaaa cgatgcgtgc cg
//
LOCUS       DATCGS010000029         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_102262, whole genome shotgun sequence.
ACCESSION   DATCGS010000029 DATCGS010000000
VERSION     DATCGS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102262"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1447)
                     /locus_tag="VIL39_00525"
     CDS             complement(65..1447)
                     /locus_tag="VIL39_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5479940.1"
                     /translation="MDKSKVKILVVDDEQGLCAGVQEALRREGYVVDAMTDSTAALKL
                     AEERLYNLVISDIKMPGLSGLELLVRVRAHSRDTLFILMTAYGTVESAVEAMKQGAYD
                     YLPKPIDLKRLRALVQRALEFQAVVAENSELRLRLQTRAESGLLVGECESMRAVARLI
                     EEVANSDVTVLIEGESGTGKEIVARSIHAQSARRNRPFISVNCAALAEQLLEAELFGH
                     VKGAFTGAIANKPGRFQLADDGTLFLDEIGDLSPKGQGDLLRVLEDGAFRMVGGSELI
                     RVNVRVIAATNKKLQEAVAAGRFREDLFYRLQIVPIVMPPLRERAEDIPLLIERFFEQ
                     LMAKHKRRPKRMSAEALQLCQRFPWPGNVRQLRNMIERLVITCREVEVGVNQLPDSLR
                     EYDRHTTTFTVRPGTSLAEVEKLLIRQTLTHVTSNRQEAAQALGISRRALQYKLKQYG
                     LLEGEERRVD"
     gene            complement(1597..2343)
                     /locus_tag="VIL39_00530"
     CDS             complement(1597..2343)
                     /locus_tag="VIL39_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5479941.1"
                     /translation="MESILAKALGQDVSQLAAAEKRDLLARLLGRLAHEVRNPLSSLD
                     IHVQLLEEDLAALAPQMREQLTPRLEIIHGELHRLEGIVERFLRLAGPAGVDLEPVAI
                     RKILTHVCELLRPEAAARGIDISTHTEDSLPPVMADPVRLTQALLNVVINAIQAVERN
                     GRVEVSASVADGRVSVAVSDNGPGIPPDKLASVFDPYYTTKNEGSGLGLWIAQQIVTA
                     HGGSLQAENRPTGGVVLTMRLPLKREAATA"
     gene            2493..3263
                     /locus_tag="VIL39_00535"
     CDS             2493..3263
                     /locus_tag="VIL39_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479942.1"
                     /translation="MKTRILPGWVLGMVLASGAITPAFAQNVIDPSPTAAPSEKTGTA
                     VLSPAVTTPAAPAPRAESASGSSPAALAAPGNPATNPPPAIRSLSPWFYEVEQLARAS
                     VEDSVIQTYITSSAGTFNLTADQVIYLKDLGVSSQVINTIIDHDQELISGARPLTASA
                     PPPYPPAVQAALAAGLHATGAATAPSSAPATSSSAPGESIVAPDDKSDLAGTWTSFEP
                     YYAPEQPASAGPVRVPYAVKLNDPIIILKLPTFTLPYW"
BASE COUNT          665 a         1335 c          955 g          674 t
ORIGIN      
        1 cgacgccacc accccctcaa cccgccaatc cgtcactcca tcactccgtc actccgctca
       61 tccatcaatc cacccgccgt tcttcccctt ccagcagccc atactgcttc aacttgtatt
      121 gcagcgcccg ccggctgatg cccaacgcct gagcggcctc ctggcgattg ctcgtgacat
      181 gcgtcagcgt ctgccggatc agcagcttct ccacttccgc cagcgaagtg cccggccgca
      241 cggtgaaggt cgtggtgtgg cggtcgtact cgcgcagaga atccggcagt tgattaacac
      301 ccacttcaac ctcgcggcaa gtaatgacca aacgttcaat catgttgcgg agctgccgca
      361 cgttgcccgg ccacggaaag cgttggcaga gctgcagcgc ctcggcggac attctcttgg
      421 gccgccgctt atgcttcgcc atgagttgct cgaagaaccg ctcgatcagc agcggtatat
      481 cctccgcccg ctcccgcagc ggcggcatga caatcggcac gatctgcaag cggtagaaaa
      541 ggtcttcgcg gaatctgcct gccgccaccg cctcctgaag cttcttattc gtagccgcga
      601 tgactcgcac attgacccgg atcagttcag aaccgccgac catccggaac gcgccgtcct
      661 ccagcacgcg caacaaatcc ccctgccctt tcggagaaag gtcgccaatc tcgtccagga
      721 acagcgtgcc gtcatcggcc agttggaacc gccccggctt gttggcaatc gcgcccgtga
      781 acgccccctt cacgtgcccg aacaactccg cctccagcaa ctgctccgcc agcgccgcgc
      841 agttcaccga tataaacggc cggttgcgcc gtgcgctctg cgcgtgaatc gagcgggcga
      901 caatctcctt gcccgtgccg ctctcgcctt cgatcagcac cgtcacgtca ctgttggcca
      961 cctcctcgat cagccgcgcc accgcccgca tggattcgca ttcgcccacc agcaggcccg
     1021 actccgcgcg cgtctgcaag cgcagccgca gctcgctgtt ctccgccacc accgcctgga
     1081 attccagcgc ccgctgcacc agtgcccgca gccgcttcag gtcaatcggc ttgggcaggt
     1141 aatcgtaagc cccctgcttc atcgcctcca cggcgctttc caccgtgccg taggcggtca
     1201 tcaggatgaa cagcgtgtcc cggctgtgcg cccgaacccg cacgagcagc tccaagccgc
     1261 tgagcccggg catcttgatg tcggaaatga ccaggttata gagccgctcc tcggctagct
     1321 tcaacgccgc cgtcgaatcc gtcatggcat ctacgacata cccctcccgc cgcaaagcct
     1381 cttgcacgcc cgcgcataga ccctgctcgt catccactac cagtatcttg actttagact
     1441 tatccatctc agcaattcaa ggagcgcgga cattcttgtc cgcagcaacg tccgtacgct
     1501 cgctggctcc gaaaaggccc atgatcggca accctctctc ccctgctgcg gacaagaatg
     1561 tccgcgctcc gtcctgtgtt catctgtggt tctctttcag gccgtcgctg cctctcgttt
     1621 cagcggcaat cgcatggtca gcactacccc gcccgtaggc cggttctccg cctgcaaact
     1681 cccgccgtgc gccgtcacaa tctgctgcgc gatccaaagc cccagaccgc taccctcgtt
     1741 tttcgtcgtg taatacgggt caaaaacgga cgccagctta tcgggtggaa tgcccggccc
     1801 gttgtcactc acggcaacgc tgacccgccc gtccgccaca ctggcgctca cttcgacccg
     1861 tccattgcgc tccaccgcct ggatggcgtt aatcaccacg ttcagcaacg cctgggtcag
     1921 acgaaccgga tcggccatga cgggcggcag cgaatcctcc gtgtgggtgg agatgtcaat
     1981 cccgcgcgcc gccgcctcgg gccgcagcag ctcgcacacg tgcgtgagga tcttgcggat
     2041 ggcgactggc tccaggtcaa ctcccgccgg tccggccagg cgcagaaaac gttccacgat
     2101 gccttcgagc cggtgcaatt cgccgtggat gatctccagc cgcggtgtca actgctcgcg
     2161 catttgcggc gccagcgcag ccagatcttc ctccagcaac tggacgtgga tgtccagcga
     2221 actgagcggg ttgcgcacct cgtgtgccaa ccgccccagc agccgcgcca gcaaatcccg
     2281 tttctctgcc gcggctaact gcgacacatc ctggcccagc gcctttgcca atatactttc
     2341 cactacccag cagcatagtg aaccccaggc cgcccgtcca gccgccaacg agaacgtgaa
     2401 acacccccca tgaataccgc gggaacgctg cgagtcgaat gttatgaacg acggttcagt
     2461 agctcaagac taccaacagc ttatgagatt atatgaagac gcgaatacta cccggttggg
     2521 ttctcgggat ggtgctggca agcggggcga tcacccccgc ctttgcccag aacgttattg
     2581 acccctcacc gacggcggct ccctccgaga aaaccggcac ggcggtcctt tccccggcag
     2641 ttacgacacc ggcggctcct gcgccccgcg ccgagtcagc ctccgggtct tcacctgcgg
     2701 cactcgctgc gcccggcaac cccgccacca acccgccccc ggccattcgc tctctgtccc
     2761 cttggtttta tgaagtcgag cagttggccc gggccagcgt agaagacagc gtgatacaaa
     2821 cctacatcac cagctccgcc ggcacgttca atctcactgc ggaccaggtc atctacttga
     2881 aggacctggg cgtctcctcc caggtgatca ataccatcat tgaccacgac caggaattga
     2941 tctccggcgc gcggcccctg acggcttctg ccccgccccc gtacccgccc gccgtccagg
     3001 cggccctcgc agccggtctc catgcgaccg gcgcggcgac cgcgccctcg tcagccccag
     3061 caacatcgtc atcagcgccc ggggaatcca tagtcgcgcc cgacgacaaa tccgacctgg
     3121 ccgggacttg gacctccttc gagccctact acgcccccga gcaaccggca agcgccggcc
     3181 cggtgcgggt gccctacgcg gtgaagctca acgacccaat catcatcctc aagctcccaa
     3241 ctttcactct cccgtactgg tagtccgaaa cgaggccgaa ccggagcaag gaaaaagggc
     3301 gtaaagtcgg cagcagcagc gggaaaaccc aaagaacttc cccaagccgc ccaccggaca
     3361 gccgttccct gccgccaagc cgcccccagc ccttcccgct ctccccaggc tctggtgtag
     3421 ccttggtgta gccttggtat agccttgggg tagccttggg gtagccttgg ggtgcctatc
     3481 agttggctat caacaggcct tggggtggct ttggtgtcgc attgatgtgg ccttggggtg
     3541 gcttgccggc ccagtcacga atcacgaatc acgaatcacc tctcgctttg ggtggctccg
     3601 cccggctgtt caaggttcga ggttcgagg
//
LOCUS       DATCGS010000030         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_250369, whole genome shotgun sequence.
ACCESSION   DATCGS010000030 DATCGS010000000
VERSION     DATCGS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250369"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2436)
                     /locus_tag="VIL39_00540"
     CDS             complement(<1..2436)
                     /locus_tag="VIL39_00540"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5479943.1"
                     /translation="MNRRDFLKTSAASGGSLLLAQTAVADPANSARKLIEPAPAPSVA
                     GPRLPDLAPARWIWFPSGRTLPNTFILFRRELHLAAKPRRATGWICADSRYRLEVNGQ
                     RVQWGPAPCDPRWAEADPLDLTDRLQAGANVLGATVLFYGAGDGTWPLGKPGFLFWLE
                     IEHADGHLEKIVSDATWQAMLCRAWPPGHPKRWYLRALQEEFDARLYPHNWSRADFQP
                     DAHWLPAMPMNGSPNKPALCTNYYEYMLDLGGGPPNTQLRPRSIPLMRESLVPAAKLA
                     ESCWLEWRITPQDYFDFRVPDAFRAQRGDVATAPAPGQWRVELDGSRGAALTFELAEQ
                     VVGWPGFAIEAPAGTTIELLVHEAHALGGPPLLNTHFDSWTRFICREGRNEFETFDFE
                     SLRWLQLHIHNAKGAVVVSDVRVRRRVFPWPNEPRIHTSEPALQRLFDASLNTLNNCA
                     QETLVDGMARERQQYSGDGGHQMHAVHLAFGERRLVARYLTTFSQGLTKDGYFLDCWP
                     AYDRLARLIERQFDLSGWGPILDHGVGFIMDCWSHYLYTGDLEPLREPYPRLLRFARY
                     LQGLTGTDGLLPVENIGIPSVWIDHAAYQRQRHKQCAFNLYAGAAMEHALAPLCRAFG
                     DASQAEAVEQFGRELVVATVSRYWSPEQGLFVNNRPWLAEEKSPRTCDRSLATAILFD
                     QCPGGQNAAAVRSLAECPREMGFSYPANAGWRLWALAKSGRADIIVADLRRRWATMAS
                     VKENNTLQEDWHAPPDSGSQWSHCAVVPLYIAYQGLLGLKPLEPGFKRFELRPQLGDL
                     EDIELTAFTIQGPV"
     gene            complement(2554..3339)
                     /locus_tag="VIL39_00545"
     CDS             complement(2554..3339)
                     /locus_tag="VIL39_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5479944.1"
                     /translation="MAFTLIELLVVIAIIAILAALLLPSLSKAKATALRAQCTSNLKQ
                     WGTAMVMYAGDNKDYFPDNSGGHDLSWMGLSLNTTFFQPYLYPNHKGTVVAGAVKQRT
                     RNDVIYCPTDDWHRISETGALDTDPILIGYFSLPGRTDNGWPYDSAGLAGWHFRKKLG
                     GPYRLAPTMSDRLQAAGTWSITANKGSVAWTTVFSGDGQTYKTASHRTTGDVPMGGNF
                     LFEDSHVDWRKFSLSNARATVDVGSMSGAWVLFYKPPNIATNL"
BASE COUNT          640 a         1064 c         1121 g          627 t
ORIGIN      
        1 caccggaccc tggatggtga atgccgtcag ctcgatgtct tcgaggtcac cgagctgggg
       61 ccggagttcg aagcgtttga agccgggctc caatggcttc aggcccagca aaccttggta
      121 cgcgatatag agcggcacga ccgcgcagtg gctccattgg gaacccgaat caggtggcgc
      181 gtgccagtct tcctgcaagg tgttgttctc tttcaccgag gccatcgtcg cccagcgccg
      241 gcgcaagtcc gcgacgatga tgtccgcgcg tccgctcttg gccagcgccc acaggcgcca
      301 gccggcgttg gcgggatagg agaagcccat ctcccgcgga cactccgcca gcgaacgcac
      361 tgccgcggcg ttctggcctc ccgggcactg gtcgaacagg atggccgtcg ccagcgagcg
      421 gtcgcaggtg cgcggtgact tttcctccgc gagccacggg cggttattga cgaagagtcc
      481 ttgctccggg ctccagtacc ggctgacggt tgccacgacc aattcccggc caaactgctc
      541 gacggcctcg gcctggctcg catcgccaaa cgcacggcag aggggcgcca acgcgtgttc
      601 catggcggcg ccggcatata gattaaaggc gcattgtttg tgccgctggc gctggtaagc
      661 ggcgtggtca atccacaccg acgggatgcc gatgttctcg accggcagca agccgtcggt
      721 gccggtcaga ccctgcaagt agcgcgcgaa ccggagcagt cgcggatacg gttcccgcag
      781 cggctcgagg tcgccggtgt agaggtaatg actccagcaa tccatgatga accccacgcc
      841 atgatccaga atcggtcccc aaccggagag atcaaactgg cgctcgatca gccgcgccag
      901 ccggtcatag gccggccagc aatccagaaa gtagccgtcc ttggtcaggc cctggctgaa
      961 ggtcgtcagg tagcgggcta ccaggcgccg ctctccaaac gccaggtgca cggcatgcat
     1021 ctggtggccg ccgtcgccgc tgtattgctg gcgctcgcga gccatgccgt ccaccagcgt
     1081 ttcctgggcg cagttgttga gggtgttgag ggaggcatca aagagacgtt ggagggccgg
     1141 ctcgctggtg tgaatgcgcg gctcattggg ccagggaaag acgcggcgcc gcacgcgcac
     1201 atcgctgacg accaccgcgc ctttggcgtt atggatgtgc agttgcagcc agcgcaggct
     1261 ctcgaagtcg aacgtctcga actcattcct gccctcgcgg cagatgaagc gcgtccacga
     1321 atcaaaatgc gtgttcagca gcggcggacc gccgagggcg tgggcctcgt gaaccaacaa
     1381 ctcgatcgtc gtgccggcgg gtgcttcaat ggcaaacccg ggccagccga cgacctgctc
     1441 ggccaactca aacgtcagcg cggcgccccg gctgccgtcc aattccactc gccactgacc
     1501 gggcgcgggc gcggtggcta catcgccgcg ttgagcacgg aaagcgtccg ggacacggaa
     1561 atcgaagtag tcctgcggcg tgatgcgcca ttccagccag cacgactcag ccagtttggc
     1621 ggcgggcacc agtgattccc gcatgagtgg aatgctgcgg ggccgcagtt gggtgttggg
     1681 cggtccgccg cccagatcca gcatgtattc gtagtagttg gtgcagagcg cgggcttgtt
     1741 gggcgagccg ttcatcggca tggccggcag ccaatgggca tccggttgga agtcggcgcg
     1801 ggaccagttg tgcggataga gtcgcgcgtc aaactcctcc tgcaaggcgc gcagatacca
     1861 gcgtttgggg tggcccggcg gccaggcccg gcacagcatc gcctgccagg ttgcgtccga
     1921 aacgatcttc tccagatggc cgtctgcatg ctcgatttcc agccagaaca ggaagcccgg
     1981 tttgcccagc ggccaggtgc cgtcgccagc gccgtagaac agaacggtgg cacccaggac
     2041 attcgccccc gcttgcagac gatcagtcag atccagcggg tcggcttcgg cccagcgggg
     2101 gtcgcacggg gctggccccc attggacgcg ctggccattg acttcgagcc ggtaacggct
     2161 gtcggcgcaa atccagcccg tcgcgcggcg aggcttggcg gccagatgca gttcgcgccg
     2221 gaagaggatg aaggtgttgg gcagcgtgcg gcccgaggga aaccaaatcc agcgcgcggg
     2281 cgcgaggtcc ggaagccttg gcccggcgac gcttggcgcg ggagccggct cgatcagttt
     2341 gcgcgcggag ttggcgggat cagccacagc agtttgcgcc agcagcagcg agccgccgct
     2401 ggcggcggag gtcttgagga agtcgcgacg attcatgcag ggaattgaac tgcggatagc
     2461 cctgtacgct ccggagacgg gtctccttcg ggataccaaa tcatgcagca aaggcgccca
     2521 ggaccggggc gaagaggccg cttgtgccaa agctcacaag ttggtcgcga tgttgggtgg
     2581 tttgtaaaac aagacccatg cgccggacat cgagccgacg tcaaccgtag cccgcgcatt
     2641 gctcagacta aatttccgcc agtccacgtg actatcttcg aacaggaaat tgcctcccat
     2701 gggaacatcc cctgtcgttc gatgactggc ggtcttatag gtctggccat cgccgctgaa
     2761 caccgtcgtc caggccacgc tacccttatt tgcggttata ctccatgtgc cggccgcctg
     2821 caaccggtcg ctcatggtcg gcgccaggcg atagggtccg ccgagtttct ttcggaagtg
     2881 ccatcccgca aggccagccg aatcgtaagg ccaaccgtta tcggttcggc ccggcaggga
     2941 gaaataaccg atcaatatag gatcagtatc caaggcgccc gtttcagaga tccggtgcca
     3001 gtcatccgtc gggcaataaa tgacatcgtt acgggtgcgc tgctttacgg cgccagccac
     3061 cacggtgcct ttgtggttcg ggtataaata gggctggaag aaagtggtgt tcaggctcag
     3121 ccccatccaa ctcaaatcgt gcccgccgga attgtccggg aagtaatctt tgttgtcccc
     3181 cgcatacatg accatggcgg ttccccactg tttaagattg ctggtgcact gggccctgag
     3241 ggcggtggcc ttcgccttgc tcagcgaggg caaaagcagg gccgccagaa tcgcgatgat
     3301 ggcaatcacc accagcagtt cgatcagggt aaacgccatc cccccgcgct cgccccgcac
     3361 ggctcgcttg ggggttgaag tggcttccag actcacctgg tagttggttg ctgtcttttt
     3421 catagactaa cttgggttgg gtgattggat tt
//
LOCUS       DATCGS010000031         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_201015, whole genome shotgun sequence.
ACCESSION   DATCGS010000031 DATCGS010000000
VERSION     DATCGS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201015"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="VIL39_00550"
     CDS             <1..2078
                     /locus_tag="VIL39_00550"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5479945.1"
                     /translation="PVLVELDGAAEFVLQIEETPDGIACDQADWVEAKITLLDGSDLW
                     LADLPLRDGERAPYSTEAPFSFVYGGKASAELLKEWKCERTSRKLDEHRTERTSVWTD
                     PGAGLQVRCVGIEYNDFPTVEWVVHFKNTGTQDTPILSDIQAVDLGLARTGQGEFVLN
                     HHAGDNCTPESYEPRRLTLEPKSEHRFAPAGGRPTSVGFPYFNLEWAGEGLIVVIGWP
                     GQWAAQFTRDAGDGLRVRGGQELTRFKLRPGEEARSPLIALQFWQGDRVRAQNVWRRW
                     MLAHNLPRTRDGKLPPPILSSCSGGFFPGLRTSEEGERQFITAFTQAGVKLDYWWIDA
                     GWYACADWPQTGTWTPDPTRYPHGFQPISELVHAKGVGLIVWFEPERVTPGTFLYTNN
                     PAWLLGHEGQQKLLNLGNAEARQWLTDHVDGMLRREDIDFYRQDFNIDPLPYWRAADA
                     PDRQGLTEMRDIEGYLAYWDELRRRHAGLLIDSCASGGRRNDLETLRRAVPLLRSDYQ
                     SFDGNSAFATGNQGHTYGLSSWIPYYGQGVYFTDRDFVYSARSYLSPAFAICADVRKP
                     GVNWPVIRRIGEQWRQVADCLLGDFYPLTSYQLNEELWLAWQFDLPERQKGIVQVFRR
                     ADSSYESARFKLRNLNPEASYVVTDLDQPAAAKEFTGRELLEHGVLISAPTQPSARIL
                     TYQMSNKRK"
     gene            2159..>2510
                     /locus_tag="VIL39_00555"
     CDS             2159..>2510
                     /locus_tag="VIL39_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEY5479946.1"
                     /translation="MLFHTLLVLALTGLFSTAGAVTPQPEELLACKTWSDGVFPAGGE
                     AGSGAFLKVLYEDAADTVARVRSWRGTPFQLGEKTYTNGLAFNSTKHILVHLSKPARR
                     FTADIGLENNDNTRQ"
BASE COUNT          471 a          794 c          800 g          445 t
ORIGIN      
        1 aaccggtcct ggtcgagctc gacggggcag cggagttcgt cttgcagata gaagaaacgc
       61 cggacgggat cgcatgcgac caggcggatt gggtggaggc aaagatcaca ctcctggacg
      121 ggagcgatct ctggctggcc gacctgccgc tgcgcgatgg ggaacgcgcg ccctactcga
      181 ctgaggcgcc gttttcgttt gtctatggtg gcaaagcctc agccgaactg ctcaaggaat
      241 ggaagtgcga gcggacctca cggaagctcg acgagcaccg caccgaacgg acgtcggtct
      301 ggaccgatcc cggtgccggc ttgcaggtgc gctgcgttgg catcgagtat aacgacttcc
      361 cgaccgtcga gtgggtggtg catttcaaga acaccggtac ccaggacacg ccgatcctgt
      421 cagacatcca ggccgtggat cttggcctgg cgcgcaccgg gcagggcgag ttcgttctca
      481 atcatcatgc aggcgataat tgcacgccgg agagctacga accccggcgg ctgaccctgg
      541 aaccgaagtc cgaacatcgc ttcgctccgg ctggcggtcg gcccacgagc gtaggctttc
      601 cctacttcaa cctggagtgg gccggcgaag ggctgatcgt cgtcatcggc tggccaggcc
      661 agtgggcggc acagtttacg cgcgacgccg gcgacggcct gcgcgtgcgc ggcgggcagg
      721 aactgacccg gttcaagctg cgtccggggg aagaggcgcg ctcgccgctc atcgcgctgc
      781 agttctggca gggcgaccgc gtgcgcgcgc agaacgtgtg gcggcgttgg atgcttgccc
      841 acaacctgcc gcgcacccgc gacggcaagc tgccaccgcc gattctcagc tcgtgcagcg
      901 gcggattctt tcccggcctc cggaccagcg aggagggcga gcgccagttc atcacggcct
      961 tcacgcaggc gggcgtgaaa ctggattact ggtggattga cgccggctgg tatgcctgcg
     1021 cggactggcc gcaaaccggc acctggacgc ccgatccgac gcgctacccg cacggcttcc
     1081 agcccatcag cgagctggtt catgcgaagg gtgtggggct gattgtctgg tttgagccgg
     1141 agcgcgtcac gccgggtaca ttcctttaca cgaataatcc ggcttggctg cttggccatg
     1201 agggccagca gaaacttctt aacctcggca acgcggaggc gcgccaatgg ctgaccgacc
     1261 acgtggatgg catgctccgg cgcgaggaca ttgacttcta ccggcaggat ttcaacattg
     1321 atcctctgcc ctattggcgc gcggctgatg cgccggaccg gcaggggctc accgagatgc
     1381 gcgacatcga aggctacctc gcctactggg acgagctgcg gcggcgccac gcgggcttgc
     1441 tgattgacag ttgcgcttcc ggcggacggc gcaacgacct ggaaacgctg cggcgcgcag
     1501 tgccgctgct gcggagcgac tatcagtcct tcgacggcaa ctccgccttc gccaccggca
     1561 accagggcca cacctacggg ctgtcctcgt ggattcccta ctacgggcag ggcgtctatt
     1621 tcactgaccg ggacttcgtc tactcggcga ggagctacct ctcgcccgcg ttcgccatct
     1681 gcgctgacgt ccgcaagccg ggagtgaact ggccagtgat acggcggatc ggggagcagt
     1741 ggcggcaggt ggcggactgt ttgctgggcg acttctatcc gctgacatcc taccaattga
     1801 acgaggagct gtggctggcg tggcaattcg atttgcccga acgccagaaa ggcatagtgc
     1861 aagtcttccg ccgcgccgac agcagctacg agtccgcccg gttcaagctc cggaacctca
     1921 atccagaagc cagctacgtc gtcaccgacc ttgaccagcc cgcggcagcc aaggagttta
     1981 ccggccgcga gttgctcgaa cacggggttc tgatcagcgc cccaacccag ccgtccgccc
     2041 gtatcctgac ttaccaaatg tcaaataaga gaaaatgaac acatacaaca tgatgactat
     2101 gagccgaaca ggttgccccg aactcagcat gtctctgatg aactgccaag cgaagcgtat
     2161 gctcttccat acgttgcttg tcctggccct gaccggcctg ttctcgaccg ccggagcagt
     2221 tacgccccaa cccgaagaac tcctcgcctg caagacctgg tcggatggcg ttttccccgc
     2281 tggtggcgag gctgggtccg gtgccttcct caaggtgctt tatgaggacg cggcggatac
     2341 ggtagcgcgg gtgcgctcgt ggcgcggcac gccgttccag cttggtgaaa agacttatac
     2401 gaacgggctc gccttcaact ccaccaaaca catcttggtg cacctgagca aaccggcccg
     2461 gcgtttcacg gcggacatcg ggctcgagaa caacgacaac acgcggcaag
//
LOCUS       DATCGS010000032         3583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_300197, whole genome shotgun sequence.
ACCESSION   DATCGS010000032 DATCGS010000000
VERSION     DATCGS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300197"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..3023)
                     /gene="mgtA"
                     /locus_tag="VIL39_00560"
     CDS             complement(354..3023)
                     /gene="mgtA"
                     /locus_tag="VIL39_00560"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEY5479947.1"
                     /translation="MKEFVPALTNPNASSQSDLARDAAGKDSAEVLRSLGTSPAGLTE
                     EEAAARLEQYGPNTVGREKHEGWLQRLYISARNPLVILLTILATISFVAPEGDARAGV
                     MMLLMLVLGLALRFIQETKADTAAAKLKAMISVTATVVRNGQARELPLQQLVPGDIVK
                     LSAGDMIPGDARLLSAKDLFVIQATLTGESMPVEKTEAPDLRGNVSAIERSNTCFLGT
                     SVESGSATAVIVATGVQTYFGKVAKSLGGRPVETAFERGVNRFTWLMIRFMLVMVPLV
                     FAIYGLTKHDWKMAFFFSLSVAVGLTPEMLPMIVSVCLSKGALAMSRKKVIVKRLNSI
                     QNFGAMNVLCTDKTGTLTMDHVILEIYCDVFKTESETVLQDAYLLSHFQTGLKNILDR
                     AVLHHQELHGALSIDKFSKIDEIPFDFTRRMMSVVVERPDGQRELLTKGAPESVFAKC
                     THFESEGDVFPMEPILVGDLIEQVNDLSEDGFRVLAVATKKVDKKAAYAKADEADLVL
                     TGYLAFLDPPKDSAAKAITVLRQHGVRVKVLTGDNDRVTRKVCTEVGINAEKIVLGSQ
                     VETMSDPQLAEAAETTDVFARLSPAHKQRIVQALQRKGHVVGFMGDGINDAPALRAAD
                     VGISVDNAVDIAKESADVILLEKSLMVLEEGVLEGRKVFVNILKYVRMGASSNFGNMF
                     SVIGASAWLKFVPMAPIQILTNNLLYDFSQVPIPTDNVGLQQIAKPRPWNMGEIAKFI
                     VFIGPISSIFDYSTYAIMWFVFKCHDLGLVPPPELAARFAQADVPNETYAAALFHTGW
                     FVESLMTQTLIIHVIRTNLLPFVQSRASWQLTMTTLLIMAVGAYLPYSPLNSTLGFVP
                     LPPLYWLLLLVTLLCYVGLTQVIKMWLIRKAWV"
     gene            complement(3270..>3583)
                     /locus_tag="VIL39_00565"
     CDS             complement(3270..>3583)
                     /locus_tag="VIL39_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479948.1"
                     /translation="QLLHVDSTGSKTWFLDSCGTTTRRDRRILLSNYDIGIAVPAHAL
                     QLGLNFAATLPEIFPGKEMGEDALRVLNDIIPLQAAIRADVAAELSHLRETAKWAARL
                     P"
BASE COUNT          707 a         1035 c         1175 g          666 t
ORIGIN      
        1 gagccaccta aagccggagg tgattcgtga ttcgtgattc gtgactgggc gggcaagcca
       61 ccccaaagcc acaattgggc aaaagctgaa atgctgaaag ctgaaagctg aaatcagaaa
      121 gagggctgca caaagccacc caatgcgaca tcaaagccac cccaaggcct gttgatagcc
      181 aactgatagg caccccaagg ctaccccaag gctaccccaa agccagttac aagccatgtg
      241 atccagtgca actccagcat ttgccatcct cgatcttcca tccgccagcc tccagctcgc
      301 gggctgcggg tccagcgggt cgggaagcac gcaaggcagc cgccggcagg agcttacacc
      361 caggccttgc ggatcagcca catcttgatg acctgggtca ggccgacgta gcaaaggagg
      421 gtgaccaaca gcagcagcca gtagagcggc ggcaagggca caaaacccag cgtcgagttt
      481 aggggcgagt agggcagata tgcgccgacg gccatgatga gcagggtggt catggtgagc
      541 tgccaactgg cgcgcgattg gacgaaggga agcaggttgg tgcggatgac gtggatgatg
      601 agcgtctgcg tcatcagaga ctcgacaaac cagccggtgt ggaacagggc ggcggcgtag
      661 gtttcgttcg ggacatccgc ctgggcgaag cgcgcggcca attccggcgg cggcacgagc
      721 ccgaggtcat ggcacttgaa cacaaaccac atgatcgcat aggtggagta gtcgaagatc
      781 gagctgattg ggccgatgaa gacgatgaac ttggcgatct cgcccatgtt ccaagggcgg
      841 ggtttggcga tctgttgcag gccgacgttg tcggtgggaa tcgggacctg ggagaagtca
      901 tagaggaggt tgttggtgag gatctggatg ggggccatgg gcacgaactt cagccacgcg
      961 ctggcgccaa tgacgctgaa catattgccg aagttcgagc tggcgcccat gcggacgtat
     1021 ttgaggatgt tgacgaagac cttgcggccc tccaacacgc cttcctccaa caccatcagg
     1081 ctcttttcca gcaggatgac atcggcggat tccttggcga tatcaacggc gttatcaacc
     1141 gagatgccca cgtcagcagc acgcaaggcc ggggcgtcgt tgatgccgtc gcccatgaac
     1201 ccaacgacgt ggcctttgcg ttggagggcc tgcacgatgc gctgcttgtg cgccggcgac
     1261 aggcgcgcga acacgtcggt ggtttccgcg gcttcggcca gttgggggtc ggacatcgtt
     1321 tcgacctggc tgccaagcac gatcttctcg gcattgatgc cgacctcggt gcacaccttc
     1381 cgggtgacgc ggtcgttatc gccggtcagc accttgaccc tcacgccatg ctggcgcagg
     1441 acggtgatgg ctttggcggc gctgtccttg ggcgggtcaa ggaaggcgag ataaccggtc
     1501 aggaccaagt cggcttcgtc ggctttggca taggcagcct tcttgtccac cttcttggtc
     1561 gcgacggcca gcacgcggaa gccgtcctcg ctcaggtcgt tcacctgctc gatgaggtcg
     1621 ccgacgagga tgggttccat cgggaaaacg tcgccctcgc tttcgaaatg ggtgcatttg
     1681 gcgaacacgg actcgggggc gcccttggtg agcagttccc gctggccatc cggccgctcc
     1741 acgacgacgg acatcatgcg gcgggtgaag tcgaagggaa tctcatcgat cttgctgaac
     1801 ttatcaatcg agagcgcgcc atgcagctcc tggtggtgca gcacagcgcg gtcgaggatg
     1861 ttcttgaggc cggtttggaa gtggctgagg aggtaggcgt cctgcagcac cgtctcgctt
     1921 tcggtcttga agacatcgca atagatttcg aggatgacgt ggtccatggt cagcgtgccg
     1981 gtcttgtccg tgcatagcac gttcatggcg ccgaagttct ggatcgagtt gagtcgtttg
     2041 acaatgacct tctttcggga catcgccagc gcgcccttgg acaggcacac cgagacaatc
     2101 atcggcagca tctccggcgt cagccccacc gccaccgaca gcgagaagaa gaaggccatc
     2161 ttccaatcgt gtttggtcag gccataaatg gcgaacacca gcggcaccat caccagcatg
     2221 aaccggatca tcagccacgt gaaccggttg actccccgct caaaagccgt ctcgaccggg
     2281 cggcctccca ggctcttggc cacctttccg aaataggtct gcacgccggt ggcgacaatc
     2341 accgccgtgg ccgaaccgct ctccacgctg gtgccgagga agcaggtgtt cgagcgctcg
     2401 atggctgaga cgttgccgcg caggtcgggg gcttcggtct tctcgaccgg catggattcg
     2461 ccggtcagcg tcgcttggat gacgaacagg tctttcgccg acaggagccg tgcgtcgccg
     2521 gggatcatat cgccggcgga gagcttgacg atgtcgcccg ggacgagctg ttggagcggg
     2581 agctccctgg cctgcccatt ccgcaccacc gtggcggtga cgctgatcat cgccttgagt
     2641 ttggcggcgg cagtgtccgc cttggtttcc tggataaacc gcagagccag tcccaatacc
     2701 agcatcagca gcatcatcac tccggcgcgg gcatcgcctt cgggcgcgac aaaggagatg
     2761 gtcgccagga tggtgagcag aatcaccagc gggttgcggg cagaaatata cagccgctgc
     2821 agccagcctt catgtttctc gcggccgacg gtattggggc cgtattgctc caggcgcgcg
     2881 gcagcttcct cctccgtcag gccggcgggc gaagtgccga gcgatcgcaa cacctcagcg
     2941 ctgtccttgc ccgccgcatc ccgggcgaga tcggactggc tggaggcatt cggattggtc
     3001 aaagcgggca caaactcttt caacggcgtg cgatcaaaga ttgtccggct tgggcagcgc
     3061 gtccacccta aagttaatgc cgtccagaat tgcgaggccc ggcggaggct ggaggcgctc
     3121 ccaggagtct ccgatgtgtg cccggcgact ctcccatgac tctcccggtg gtccagcaac
     3181 agtgtggctt atggaacggg gcctattcgc gctcgctacg ccatccatgg ccggttggga
     3241 cggaagcgtt ttccagtcca aactagaagt catggcaggc gggcggccca tttggcggtt
     3301 tcgcggaggt gggagagctc ggcggcgacg tcggcgcgaa tggcggcctg gagcgggatg
     3361 atgtcgttca gaacacgcag ggcgtcttcg cccatctcct tgccggggaa aatctcgggt
     3421 aaggtggcgg cgaagttcag gccgagctgg agcgcgtggg ccgggacagc gatgccgatg
     3481 tcgtaattgc tgaggagaat ccggcggtcg cggcgggtag tggtgccgca ggaatcgaga
     3541 aaccaggtct ttgagccggt ggaatccacg tggaggagct gga
//
LOCUS       DATCGS010000033         4163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_597464, whole genome shotgun sequence.
ACCESSION   DATCGS010000033 DATCGS010000000
VERSION     DATCGS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597464"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VIL39_00570"
     CDS             complement(<1..304)
                     /locus_tag="VIL39_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY5479949.1"
                     /translation="MTAIAPGREARKVILHEVGLRDGLQMEKQVVPLEQKIRWTEPLL
                     AAGVDIIQLGSFVHPVKVPQMADTDVLFRHFSVPGRKPSGAVLSGLVLNEKGLERGM"
     gene            complement(301..1419)
                     /locus_tag="VIL39_00575"
     CDS             complement(301..1419)
                     /locus_tag="VIL39_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY5479950.1"
                     /translation="MAKYKIAWLPGDGIGVDVLEAAKIVLDKVQLEAAYLPGDIGWDF
                     WCQEGDAFPPRTIELLKNVDAALFGAITSKPAKTAAAELAPALQNKGLSYRSPIVRMR
                     QLFDLYICLRPCKGYPGNPLNFKEGIDLVVYRENTEDLYAGVEFAPVPQELADTLSKL
                     AKPFAPFAKLPLDQYAVSCKINTRAGSERIARAAFEFARQHKRKKVTIVHKANVVRAT
                     DGLFLEAAKAVAKDFPDIKMDDANIDAMTMWLLKNPFNYDVLVAPNLYGDIISDLCAQ
                     MVGGLGFGCSGNIGEKLAVFEPTHGSAPKYAGQYKVNPIATILAVKMMLDWLGESKKA
                     VALESAVAQVIKEGKVRTYDMGGSSTTLQMAQAIAAKL"
     gene            1645..>4163
                     /locus_tag="VIL39_00580"
     CDS             1645..>4163
                     /locus_tag="VIL39_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY5479951.1"
                     /translation="MRAAERFLELVPVQTRERGRYYHATGHVLKVACLKPDQAYTAIV
                     RGNNDYAVRLTYSEQTWTSDCSCPMGYDCKHTVAAMLELQALWAKDSPATPATVSASS
                     NKPSKKKSARVFKPFVPQPPASPLYQRLVKNLGRPLNYPEAGFVRKVQMLYANAESRY
                     LTESDLNQLFPQHFSYGWTALDLWPKRPKDDYELWLYLAAEFRRRKIPLPDYMAAITD
                     FTTLEAAIRKWEREKEVAHWQAWFETNETSVPPAGDTLDLRLAFLAEEARLQWRTQPD
                     MPFADLKQAQAKRFSESVEHGTLTLTPDAMLFWGALHKPWGYESWWTIRYSSEASCLP
                     LNRLLRLPLPPERFVNANGEPLARPAEPLRLQLHPPPEGGDDYELRLTTADGQEPPPI
                     HFVLSGRPTLYITETAIYAGPPRNALERDSRKVIPAPALETPGGIRFLHALNLPLPEH
                     LAQRTKVVPVAVTLACALKESYPGSKSESIVLRATARAEGMEEEEFALNGWQTPNQGW
                     RPIKAAKPVKGWIPLYDRSAQAHFPRVLEPLAAKYANYGGGWTLRLTKKTPELFVAWL
                     QTIPPEITVELDRELATLRDAPISGTVSLDVQEAGIDWFDLKVILNVADLVLTPEETK
                     LLLNARGGYVRLGEKGWRRLQFNLTPEDDEQLARLGLAPRDFTAEPQRFHALQLADKA
                     AKKFLTPERVEQIHRRVSELKTRVSPTVPEPIRAELRPYQVEGYHFLAYLTTNRFGGV
                     LADDMGLGKTLQALVWIAWLRAEANGKAQPSLVVCPKSVTDNWVAEAEHFYPSLRVRR
                     WTGEGADALSAARDGSDIIVINYAQLRSLSPSIAQVTWQAA"
BASE COUNT          810 a         1415 c         1208 g          730 t
ORIGIN      
        1 ccataccgcg ctccaggccc ttctcgttga gcaccaggcc cgagagcacc gcgccggacg
       61 gcttacgccc cggcactgag aaatgccgga acaggacatc agtatccgcc atttgcggca
      121 ccttgaccgg atgcacgaac gagccgagct ggatgatgtc caccccggcc gccagcaggg
      181 gctcggtcca gcgtatcttc tgttcgagcg ggacgacctg cttctccatc tggagaccgt
      241 cacgcagccc gacttcgtgg aggataacct tccgcgcctc ccgtccggga gcaatggccg
      301 tcatagcttc gcggcgatcg cttgcgccat ttgcagggtc gtgctcgacc cgcccatatc
      361 gtaggtgcgg accttgcctt ccttgatgac ctgggccacg gcgctctcca aggcgacggc
      421 cttcttcgac tcgcccagcc aatcgagcat catcttcacg gccaggatgg tggcgatcgg
      481 gttcactttg tattggcccg cgtattttgg cgccgagccg tgcgtaggct cgaatacggc
      541 cagcttctcg ccgatattgc ccgagcaacc aaagcccagg ccgcccacca tttgggcgca
      601 cagatcggaa atgatgtccc cgtagaggtt cggcgcgacc agcacgtcgt agttgaaggg
      661 gttcttaagc agccacatcg tcatggcatc aatgttggcg tcgtccatct tgatgtcggg
      721 gaagtccttc gcgacagcct tggcggcttc gaggaacagc ccgtccgtcg cgcgcacgac
      781 gtttgccttg tgcacgatgg tgaccttctt gcgcttgtgc tgccgcgcga actcaaacgc
      841 cgcccgggca atgcgctccg atcccgcgcg ggtattgatc ttgcaggaga cggcgtattg
      901 atccaggggg agtttggcaa agggcgcaaa gggcttggcg agcttcgaga gcgtatccgc
      961 cagctcctgc ggcacgggcg caaactcgac gccggcatac aggtcctccg tgttctcgcg
     1021 ataaaccacc aggtcaatgc cttccttgaa attcaacggg ttgccagggt agcccttgca
     1081 gggccgcagg cagatataga ggtcaaacaa ctgccgcatc cgcacgatgg gcgaacggta
     1141 gctcaacccc ttgttttgta gcgcgggcgc aagctcggcg gcggcggtct tggcgggttt
     1201 ggaagtgatg gcgccgaaca gcgcggcgtc cacatttttc agcagctcaa tggtccgcgg
     1261 cgggaacgca tcaccctcct ggcaccagaa atcccagcca atatccccgg gaagatacgc
     1321 agcctcgagt tggaccttat cgaggacaat cttggctgcc tcaagcacat ccacgccgat
     1381 gccgtcgccc ggcaaccatg caatcttgta ctttgccata aaatatacgt gtgcgcctga
     1441 tgaaaccgcc ttacggccca ttcgtcaaga aagggaaggc gcgggtcccg accatcgggg
     1501 cgcatcttgc gggaaccgcc aaccagagaa agcgcatggc tgaaggagat gccagacata
     1561 atccacagtg aacaaactca cgcttgtccg cggacgcatc ctgcttcatc gttaccgctg
     1621 caaatgagcg aacgagacaa attgatgcgc gcagcggaac ggttcctgga actcgtgccg
     1681 gtgcagacgc gcgagcgcgg ccggtactac catgccaccg gccacgtgct caaggttgct
     1741 tgcctcaagc ccgaccaggc ttacaccgcc atcgtgcgcg gcaacaacga ttatgccgtc
     1801 aggctgacct attcggaaca gacctggacg tcggactgct cttgcccaat gggttacgat
     1861 tgcaagcata ccgtcgccgc gatgcttgag ttgcaggcgc tgtgggccaa ggattctccg
     1921 gcaacgcctg ccacggtatc ggcctccagt aataaaccct caaagaagaa atccgcgcgc
     1981 gtcttcaaac cattcgtgcc gcaaccgccg gcctcgccgc tttaccagcg tttggtaaaa
     2041 aacctcgggc gcccactgaa ctaccccgaa gccggcttcg tccgaaaggt gcaaatgctc
     2101 tatgccaacg ccgaatcccg ctatcttacg gaaagcgacc tcaaccagtt gtttccccag
     2161 cacttcagtt atggctggac ggccctcgat ctgtggccga agagacccaa ggatgactac
     2221 gaactctggc tctacctggc cgcggaattc cgccggcgga agattcccct gcccgactac
     2281 atggcggcca tcaccgattt caccacgctg gaagccgcca tcaggaagtg ggagcgcgag
     2341 aaggaggtcg cgcattggca ggcctggttt gagaccaacg aaaccagcgt gccccccgcc
     2401 ggggacacgc tcgacctgcg attggcgttc ctcgccgagg aagcccgctt gcaatggcgc
     2461 acccagcccg atatgccgtt tgccgatctc aagcaggcgc aggctaaacg cttcagtgag
     2521 tcggttgagc acggcacgtt gaccctcacg ccggacgcga tgctgttctg gggtgccctc
     2581 cacaagccgt ggggctacga aagctggtgg accatccgct acagctcgga ggcctcctgc
     2641 ctgccgctca accggttgct ccgcctcccg ctgccgcccg agcgtttcgt gaatgcaaac
     2701 ggggaaccgc tcgcgcgccc cgccgaaccg ctccgcctcc agttgcatcc gccgcccgag
     2761 ggcggagatg attacgagct ccgcctcacc accgccgacg gtcaggagcc gccgcccatt
     2821 catttcgtcc tgagcggacg ccccacgctc tacatcaccg aaacggccat ttacgccggc
     2881 ccgccccgga acgcgctcga acgtgactcg cgcaaagtca ttcctgcgcc cgccctggaa
     2941 actcccggcg gcattcggtt tctgcacgcg ctgaatttgc cgctgcccga acacctcgcg
     3001 cagcgcacca aagtcgttcc cgttgccgtt accctggcct gtgcgctcaa agaaagctat
     3061 ccgggcagca aatcggaatc catcgtcctg cgcgccaccg cccgggcgga aggaatggag
     3121 gaggaggagt tcgccctgaa cggctggcaa acgcccaatc agggctggag gccaataaag
     3181 gctgccaagc cagtgaaagg gtggatcccc ctttacgacc gctcggcgca ggcgcacttt
     3241 ccccgcgtgc tggagccgct cgccgccaaa tacgcaaact acggcggcgg ctggacgctc
     3301 cggctaacaa agaagacgcc cgagcttttc gttgcgtggc tgcaaaccat ccctcccgaa
     3361 atcacggtcg agctcgaccg cgaactcgcc accctccgcg acgcgcccat cagcggtacc
     3421 gtgtccctgg acgtgcagga ggccggcatt gattggttcg atctcaaagt catcctcaac
     3481 gtggccgacc tcgtgctcac gccggaggaa accaaattgc tcctcaacgc gcgcggcggc
     3541 tacgtgcgcc tgggcgagaa gggctggcgg cgcctgcagt tcaacctcac gcccgaggac
     3601 gacgagcagc tcgcccggct cggcctcgcc ccgcgcgact ttaccgccga accccagcgt
     3661 ttccacgccc tgcagctcgc ggacaaggcc gccaagaaat tcctcacgcc ggagcgcgtc
     3721 gagcaaatcc accgccgggt cagtgaattg aagacccgcg tctcgccgac ggtcccggag
     3781 ccgatccgcg ccgagctgcg cccgtatcag gtcgagggct accacttcct cgcctacctc
     3841 accacgaatc gtttcggagg cgtgctggcc gacgacatgg gcctcggcaa gaccctgcaa
     3901 gccctggtgt ggatcgcatg gttgcgcgcc gaggcaaacg gcaaagccca gcccagtctc
     3961 gtcgtctgcc ccaagtccgt caccgacaac tgggtcgccg aggcggagca cttctatccc
     4021 agcctgcgcg tccgccgctg gacgggtgaa ggcgccgacg cgctgagcgc cgcgcgggac
     4081 gggtccgaca tcatcgtcat caactacgcg caactccgct ccctctcgcc gtccatcgct
     4141 caagttacct ggcaggcggc gat
//
LOCUS       DATCGS010000034         8335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_399326, whole genome shotgun sequence.
ACCESSION   DATCGS010000034 DATCGS010000000
VERSION     DATCGS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399326"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VIL39_00585"
     CDS             complement(<1..834)
                     /locus_tag="VIL39_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479952.1"
                     /translation="MDEQAPNYHREFLKSPQHAALGLLTLGVGFMSGMMLPLIAGITC
                     YSLGWIYLPDLPFFRRWVDRRREAAKRAEEMQKVAAFIQRRDALIASLSPERRDCYTR
                     LAAVCRDIENASAENLLASAGPATDPRLRKLDELMWTYLRLLGIQESLDQFLETERSE
                     DVPAILKDAEAEAARLNTEVEALKAKGNTAALDTKQRYLGSRLERLEVLRKRQQRSEQ
                     AEANLAFVVSEQERLDQQVKLIRADAVATKNAETLTARIDATVEHLDQTNKWLSEMDE
                     FK"
     gene            complement(914..1825)
                     /locus_tag="VIL39_00590"
     CDS             complement(914..1825)
                     /locus_tag="VIL39_00590"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5479953.1"
                     /translation="MPEATHPPEGAAPQHAKPNHAEALLAAPPQQHLLAPPDVVTFKH
                     VSKTFGHGPDAKLALQDINFTIEDLPNVGELITLVGPSGCGKSTLLRIIAGLKPHFPQ
                     TSGEVLVLDKPVQEAGPDRGLVDQRYSLLPHLRVIENVAFGLKLRGVNRSERVARAQE
                     WVKKVGLEGAEKKFPSELSGGMQQRVAIAATLILQPRILLMDEPFGALDPGIRLRMQE
                     LLIQLWNEQQSTVFLVTHSVEEAVYLGDRVFLMAPNPGRLVDVLKVPRPDESPEASRR
                     KPWFIDFCQALLRRLEEESPTPHGVKV"
     gene            complement(1829..2710)
                     /locus_tag="VIL39_00595"
     CDS             complement(1829..2710)
                     /locus_tag="VIL39_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5479954.1"
                     /translation="MTDLRPLLGFLVFALVFGTLLTLVVRLARLHVRGELDYSRMLGL
                     TALFVGVVLAVWWFLTRGQIGERIVQPLILPSPMEVLRSFVPLHVEQGLVRSAITSWM
                     RVTTGFGLAALVALPLGVYMATFSSVAAFFRPLALAGAYVPIVVFIPLSMTWFGTGET
                     QKIGFLFIGCFVALLPLVIKDIADVPSAFLDVAVTKGASQWQLVRHVLFPVAQANIWD
                     HLRGVYGVGWGWIIMAEVYVRPQYGLGALIGISERRSQTASIFAVIIVIVLIAVACDQ
                     LWRLGGELLFPYRKTDK"
     gene            complement(2902..4605)
                     /locus_tag="VIL39_00600"
     CDS             complement(2902..4605)
                     /locus_tag="VIL39_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HEY5479955.1"
                     /translation="MLLAVVGFGVYRWWDKIAPVSKPQNVSVDVAKVKEEIARAKAVP
                     ADIPLLAGTNAATLVERSGIPAVTGVSDYKKETKDGKPVIEFPINVWPGWAPIIMANA
                     GMEPNDQSVFAKKYGFYVHLAIVDDPVKARDLFASGQSHILWGTLDMIALFAPELVKD
                     SRTVPVVCQQVDFSAGGDGMVARGEIRSINDLRIKDGKRKKVVLAQNSPSHYFIMSLL
                     IDAGIDPGDIDFKWSADAPSAAKIFVQDPSYDAFVGWSPDIYNVTDKLKGSRLVVTTG
                     TANHLIADVWAVRNDFYRDHPEIVSGLVRGIFEGVDMVRKDPSRAAQALAAAFSLPVE
                     DCKNMVGKDGGISEGDAHLTNYRENAKFFLDPFNPANFEVVWNSASTIYKSLGTISSS
                     VPVAKVKAATVLGAMSEAYKDVRDLSQPTFKPDALMKMSAEAGAGQVLTKAVMIAFEP
                     NKSGLNAEYDTTIPATLEEIGKLAGRFGNAYIIIEGNTDASRKGIVPTDLVRQLSYDR
                     ADAVRKAIMEKYQFDPNKFKVVGNGWDNPLPGCTDPSNSEHNKKNRRVEVKVFPLETG
                     S"
     gene            complement(4781..5539)
                     /locus_tag="VIL39_00605"
     CDS             complement(4781..5539)
                     /locus_tag="VIL39_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479956.1"
                     /translation="MFRAIKRLFIWMGLMAEKATETDAINQAVVERGIRDSKAKADKA
                     HYANGQLAGQIALLRDQIKRQERQREELQSLLQAAAAANDEGNGSHYAEELATLEADL
                     NDNKAQADNLEQLYKQNTSIIAESLREIQRFQREFEAVKAKVAIGRSLEGLAGMMKSS
                     ITELQGMMGGELGQSMQQLRQSAASGEGQMRATLDLAKEMGSGVARQQDARKLRGAAL
                     FQEYKKKMGMVQPEAAAATPAPTAQPERQKIPEQ"
     gene            complement(5557..6270)
                     /locus_tag="VIL39_00610"
     CDS             complement(5557..6270)
                     /locus_tag="VIL39_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY5479957.1"
                     /translation="MKIILSLAVMTALVALEQGTTCRAAEQDGVALAIIYDTSGSMHD
                     AVRGSTGKSSPKYVIANRALSAVAKQIQAFASSNASGAPRKVEAGLFVFSGESVREAV
                     KFGPFDAEALEQFARSFSSPSGNTPLGNALTVATRKVLSSPLSRKHVLIITDGMNTAG
                     PTPSEVMPRLRQQAQEKQATVSVHFVAFDVDAKVFAPVKKLGATVVGASDEKQLNTQL
                     EYILQRKILLEEEEPVKKK"
     gene            complement(6466..7209)
                     /locus_tag="VIL39_00615"
     CDS             complement(6466..7209)
                     /locus_tag="VIL39_00615"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HEY5479958.1"
                     /translation="MNKQKWIIVILALGLMGGTAALLTRMQATQRLGQPAVKTSPIPN
                     SQRLQVQLPERVLDCTSEAVEVDKTTLGWLPQDTSFGQRIYKAPDGFQTSVNVVLMGR
                     DRTSLHKTEFCLEGQGWVIDRNATSQATVRMDRPYPYDLPVTKFVATKQVTVQGQPVT
                     ARGIYVLWFVADNDEYTSRHWRRMWWMARDLLRTGVLQRWASIGYFATCAPGQEDATF
                     ERLKKFIVASAPEFQLVPRSAGAARASAP"
     gene            complement(7232..8221)
                     /locus_tag="VIL39_00620"
     CDS             complement(7232..8221)
                     /locus_tag="VIL39_00620"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEY5479959.1"
                     /translation="MANQPENGILEDFRIQFMECWNRLPNKGLFLGLLVAWLALFQFL
                     GNSTLGYISTPSLLEWMHTVYSGEDASGSDDGHGKFIPFVVLALFWWKRKQLLALPLK
                     TWWPGLLLVALALVLHLFGYLAQQTRISIIALFAGIYGLMGLAWGRQWLRESFFPFFL
                     FAFCVPLGWSAVSITFPLRVLVCKLVVLISNYILQIDVARVGTALMDPSGAYQYDVAA
                     ACSGIRSLFATIAVAIIYAMLCFRPWWKRGLLIAAAVPLAVLGNTIRMLSIIIAAEIW
                     GQKGGSYVHDGGPYGIISLLPYVAAFGGLMLLGYWLQESPSKPVAPPTTAPAT"
BASE COUNT         1565 a         2616 c         2538 g         1616 t
ORIGIN      
        1 cttgaactca tccatctcgg agagccattt gttagtctgg tctaggtgct cgacggtcgc
       61 gtcaatgcgg gcggtgaggg tctcggcgtt cttggtggcg acggcatcgg cgcggatgag
      121 cttgacctgc tggtcgaggc gttcctgctc ggagacgacg aaggcgaggt tggcctcagc
      181 ctgctcgctg cgttgctgac gtttgcgcag cacttcgagc cgctccaggc gggagcccag
      241 atagcgttgc ttggtgtcca gggcggcggt gttacctttg gctttgaggg cctcgacttc
      301 ggtgttgagg cgggcggctt cggcttcggc gtccttcaaa atggccggca cgtcttcact
      361 gcgctcggtc tccagaaatt ggtccagcga ctcctggatg cccagcaggc gcaggtaggt
      421 ccacatcagc tcgtccagct tgcgcaagcg cgggtcggtg gcggggccgg cggaggccag
      481 cagattttcc gcgctggcat tctcgatatc gcggcaaacg gcggcgaggc gggtgtagca
      541 gtcacgccgc tccggcgaga gcgaggcgat aagggcgtcc cggcgctgaa taaacgccgc
      601 gaccttctgc atttcctccg cgcgtttggc ggcttcgcgg cggcggtcca cccagcggcg
      661 gaagaacggc agatcgggaa gatagatcca gcccagcgag taacaggtta tcccggcgat
      721 gagcggcagc atcatcccgc tcatgaagcc cacccccagc gtcagcagcc caagcgccgc
      781 gtgctgcggg ctcttcagaa actcgcggtg atagttgggc gcttgctcgt ccatgcggtt
      841 ctaggcaggc agtttagtgc atgcaattgt tcgcgaacac acaaaaatta agcgctctgc
      901 gctccggccc gcgctaaacc ttgactccat gcggcgttgg tgattcttcc tcgagccgcc
      961 gcagcaacgc ctggcagaaa tcaatgaacc acggtttgcg ccggcttgct tcgggggact
     1021 cgtccgggcg cggcaccttc agaacgtcaa ccagccgccc cgggttgggg gccatcaaga
     1081 acacccggtc gcccaggtaa accgcctcct cgacggaatg ggtcaccagg aacaccgtgc
     1141 tctgctgttc attccacagt tggatgagca gctcctgcat gcgcaaccgg atgcccgggt
     1201 ccagggcgcc gaaaggctcg tccatgagca ggatgcgcgg ctggagaatg agcgtggccg
     1261 cgatggccac ccgctgctgc atcccgccgg agagctcgga aggaaacttc ttctccgccc
     1321 cttccaggcc gacctttttc acccattcct gcgcgcgggc gactcgctcc gagcggttga
     1381 ccccgcgcaa cttcagcccg aaggcgacgt tctcgatcac acgcaggtgt ggcagcagcg
     1441 agtaccgctg gtccaccagg ccgcggtccg gccccgcttc ctggaccggc ttgtccagca
     1501 cgagcacttc gccgctggtc tggggaaaat gcggtttcaa gccggcgatg atgcgcaaga
     1561 gggtggactt cccacagccc gaaggcccga cgagggtgat caactcgccg acgttgggca
     1621 ggtcttcgat ggtgaagttg atgtcctgaa gggcgagctt ggcatccggg ccgtggccga
     1681 aggtcttgga gacgtgcttg aaggtgacca cgtccggcgg cgccaggagg tgctgttgcg
     1741 gtggagccgc gagcagggcc tcggcgtgat tgggcttcgc atgctgagga gcggctcctt
     1801 cggggggatg agtggcttcc ggcataaatc acttgtcggt cttgcggtaa ggaaagagca
     1861 attcaccccc caggcgccac aactggtcac aggccacggc gatgagcacg atgacgataa
     1921 tgacggcaaa gatggaggcc gtttggctgc gcctttccga gatgccaatc agggcgccca
     1981 gcccatactg gggccgcaca tagacttccg ccatgatgat ccagccccaa ccgacgccat
     2041 acacgccgcg cagatggtcc cagatattgg cctgtgcgac gggaaacaac acgtgccgca
     2101 caagctgcca ctgtgatgct cccttcgtga ccgccacatc caggaaggcg gacggcacgt
     2161 ccgcgatgtc cttgatgacc agcggcagca gcgcgacaaa gcagccgatg aagaggaatc
     2221 cgatcttctg ggtctcgccg gtgccgaacc acgtcatgct cagcgggatg aagaccacaa
     2281 tcggcacata ggcccccgcc agggcgaggg ggcggaaaaa ggccgcgacg gaggaaaagg
     2341 tggccatata aacccccagc ggtagtgcga caagggcggc gagtccaaaa ccggtcgtta
     2401 cgcgcatcca cgaggttata gcgctgcgca cgaggccctg ttcgacatgc aggggcacga
     2461 aggaccgcag cacctccatg gggctgggta gaatgagggg ctgaacgatt ctctccccta
     2521 tctggccccg ggtgagaaac caccacacgg ccaggaccac ccccacgaac agggctgtca
     2581 ggccgagcat ccgggagtaa tccagctccc cgcgaacgtg gagccgagcc aggcgcacca
     2641 ccagcgtcag cagcgtgccg aacaccagcg cgaaaaccag gaagccaagt aaaggtcgca
     2701 agtcggtcat ggggttctag ctcaaataat acagcgcaac cacagatgga cacagataaa
     2761 cacagataag aaccactcaa aaccgcgctt cactattgcc agtatccgct cgtcgcacag
     2821 tgagccagag gaaagcaaga ggggagtttg gccctcagaa tctgtgttca tctgtgtcca
     2881 tctgtggttc tgtgcttcgg tttacgaccc ggtttccagc gggaacacct tgacctcaac
     2941 gcgccggttc ttcttgttat gctccgagtt gctcgggtcg gtgcagccgg gcagcggatt
     3001 gtcccagccg ttgccgacca ccttgaactt gttcgggtcg aactggtact tctccatgat
     3061 ggccttgcgc acggcgtcgg cacggtcgta ggagagttgc cggaccaggt cggtggggac
     3121 gatgcctttg cggctggcgt cggtgttgcc ctcgatgatg atgtaggcgt tgccgaaccg
     3181 gccagcgagc ttgccaatct cctccaacgt cgccgggatg gttgtatcat actccgcgtt
     3241 gagtcccgat ttgttcggct cgaaggcaat catcaccgcc ttggtcaaga cctggccggc
     3301 accggcctca gcgctcatct tcatcaacgc atcgggcttg aaggtcggct gggagaggtc
     3361 gcgcacatcc ttgtaggcct cggacatggc ccccaggacc gtcgccgcct tgactttggc
     3421 caccggcacc gagctgctga tcgtgcccaa ggacttatag atggtcgaag cgctgttcca
     3481 cacgacttca aagttcgccg ggttgaacgg gtcgaggaag aacttcgcgt tctcgcggta
     3541 gttcgtcagg tgggcatcgc cctccgaaat gcccccgtcc ttgccgacca tgttcttgca
     3601 atcctccact ggcaggctaa aggcggcagc cagcgcctgg gcggcccgac tgggatcttt
     3661 gcgcaccata tccacccctt caaagatgcc gcgcaccagg cccgacacga tctccgggtg
     3721 atcgcggtaa aaatcgttgc gcacggccca cacgtcggcg atcaggtggt tcgcggttcc
     3781 cgtcgtcacc accaggcgcg atcctttcag cttgtccgtc acattataga tgtccggcga
     3841 ccagcccacg aacgcatcgt agctcgggtc ctgcacaaag atcttcgccg ccgaaggcgc
     3901 gtcggcagac cacttgaaat caatgtcccc cgggtcgatg cccgcgtcga tgagcagcga
     3961 cataatgaag taatggctgg gcgagttctg cgccagcacc accttcttgc gcttgccgtc
     4021 cttgatccgc agatcgttga tgctgcgaat ctccccgcgc gcgaccatgc cgtcgccgcc
     4081 cgccgagaag tccacctgct ggcacaccac cggcaccgtg cgcgagtcct tgaccagctc
     4141 cggcgcgaat agcgcgatca tatccagcgt gccccacagg atgtggcttt ggccgctggc
     4201 gaagaggtcg cgcgccttca ccgggtcatc cacgatggcc aggtgaacat agaagccgta
     4261 tttcttggca aagacgcttt ggtcattcgg ttccatgccg gcgttggcca tgatgatggg
     4321 cgcccagccg ggccagacgt tgatagggaa ctcgatgacc ggcttgccgt ctttcgtctc
     4381 cttcttgtag tcgctgacgc cagtcaccgc gggaatcccc gagcgctcga ccagcgtggc
     4441 cgcgttggtg ccggccagca gggggatgtc cgccggaacg gctttggccc tggcgatttc
     4501 ctctttcacc ttggcgacgt caacggagac gttctgcggc ttgctcacgg gcgcgatctt
     4561 gtcccaccag cgatacaccc cgaagccgac cacggccagg agcacaattg ttaaaactat
     4621 ctttccacgt gttgtcatag cattaagggg attggggtgc tggagtgaga gagtgataga
     4681 gtgatgggaa gaatcccaat actccagcat gcctcgactc caaccccggg ttcatttgtc
     4741 agtgcattac tccattactc catcactccg tttgttcttt ttactgctct ggaatcttct
     4801 gccgttccgg ttgcgctgtc ggggctggcg ttgccgccgc cgcttccggt tggaccatgc
     4861 ccatcttctt cttgtattcc tggaacagcg ccgcgccgcg gagtttgcgg gcgtcctgct
     4921 gacgggcgac gcccgagccc atttccttcg ccaggtcgag cgtcgcgcgc atctggccct
     4981 cgccggacgc tgcggactgc cggagctgct gcatggactg gcccaactcg ccgcccatca
     5041 tgccctgcaa ttcggtgatt gaactcttca tcatgccggc caggccttcg agcgagcgcc
     5101 cgatggccac cttggctttg accgcctcga attcgcgctg gaacctctga atctcgcgca
     5161 ggctctcggc gatgatcgac gtgttttgct tgtaaagctg ctccaggttg tccgcctgag
     5221 ccttgttatc gttcaggtcg gcttccagtg tggccagttc ctcggcgtaa tgcgagccgt
     5281 tgccctcgtc attagccgcc gccgccgcct gcagcaggct ttgcagttcc tcccgctggc
     5341 gctcctgccg tttgatctgg tcccgcagca gggcgatctg cccggccagt tggccgttgg
     5401 cgtaatgcgc cttgtcggcc ttcgccttgg agtcgcggat gccgcgctcg acgacggctt
     5461 ggttgatggc atcggtttcg gtggccttct ccgccatcag gcccatccat ataaacaacc
     5521 gcttgatcgc tctgaacata ggttttcttt ctgttgttac ttctttttca ccggctcctc
     5581 ctcttcgagg agaatcttgc gctggaggat atactccaac tgcgtgttga gttgcttctc
     5641 gtccgacgcg ccaaccaccg tcgcgccgag cttcttgact ggggcaaaaa ccttggcgtc
     5701 cacgtcaaac gccacgaagt gcacggacac cgtcgcctgc ttctcctgtg cctgctgcct
     5761 gagccggggc atgacctctg aaggcgtcgg tcccgccgtg ttcatgccgt cagtgatgat
     5821 cagcacgtgc ttgcgggaca ggggggagct caggaccttc cgggtggcga cagtcaaggc
     5881 attgcccagc ggcgtgttgc cgctggggct ggaaaagctc cgggcaaact gctccagcgc
     5941 ctccgcatcg aacggcccga acttcacggc ctcccgcacg ctctccccgc tgaacacgaa
     6001 caggccggct tcgaccttcc ggggcgcgcc gctggcattg ctgctggcaa aggcttgaat
     6061 ctgcttggcc accgccgaca gggcgcggtt ggcgatgaca tatttgggag aggacttgcc
     6121 ggtgctgccg cgcaccgcat cgtgcatgct gccggaagta tcgtaaataa tggccagcgc
     6181 gacgccgtct tgctcggcgg cgcggcaagt tgtgccctgc tccagcgcaa ccagagcagt
     6241 cataacggcg agcgatagaa tgatcttcat attcaaccta agcctttctc ccactgctaa
     6301 ccgcacgcaa caacgtagga ttagcaattg gattacagcg tgtcaactat ccctttctgt
     6361 ggcccccacc gcatctataa aagctgcagg tgtttgtaac ccgcctcgca atccgtcccc
     6421 acggcgtcgg gcatgatgtt tccgagcgat ccctggtgat gcagctcagg gagcggaagc
     6481 gcgggcggca cctgcggacc gcgggacgag ttggaactcg ggcgccgagg cgacgataaa
     6541 cttcttcagc cgctcgaagg tggcgtcctc ctggccgggg gcgcaggtgg cgaaatagcc
     6601 gatcgaagcc cagcgctgca aaacgcccgt gcgcaacagg tcccgggcca tccaccacat
     6661 ccgccgccaa tgccgcgagg tgtactcgtc attgtcggcc acaaaccaaa ggacataaat
     6721 accgcgggcg gtcacgggtt ggccttgaac ggtaacctgc ttggtcgcca cgaacttcgt
     6781 caccggcaga tcgtaggggt aaggccggtc catccgcacg gtcgcttgcg aagtggcgtt
     6841 gcgatcgatc acccagcctt gcccctccag gcagaattcg gtcttgtgca ggctggtgcg
     6901 gtcgcggccc atgagcacga cgttcacgga ggtctggaat ccatccggcg ccttatagat
     6961 gcgctggccg aagctggtgt cctgcggcag ccagcccagc gtggttttgt ccacctccac
     7021 cgcttccgag gtgcagtcca gcacgcgctc gggcagttgg acctgaagcc gttggctgtt
     7081 cgggataggg ctggtcttca cggcgggctg gccgagcctt tgggtggctt gcatccgcgt
     7141 gagcagcgcc gcggtgccgc ccatcaggcc gagcgccaga attacgataa tccacttctg
     7201 cttgttcata gatgccggga ggcgcgccgg gtcaggtggc cggtgctgtc gtgggcggcg
     7261 cgacgggttt ggacggactc tcttgcagcc aatagcccag cagcatcaac cccccaaacg
     7321 ccgccacgta gggcagcagg ctgataatgc cgtaaggccc gccgtcatgc acgtaagacc
     7381 cgcccttctg cccccagatt tccgccgcaa taataatgct gagcatccgg atggtgtttc
     7441 ccaggacggc tagcggcact gctgcggcaa tcagcaaccc gcgtttccac cagggccgga
     7501 agcagagcat ggcatagata atcgcgaccg cgatggtggc aaacaggctg cgaatcccgc
     7561 tgcaggccgc cgccacgtca tactggtagg cgccgctcgg gtccattaac gccgtgccca
     7621 cccgcgcgac atcgatctgc aggatgtaat tggaaatcaa caccaccaac ttgcacacca
     7681 gcactcgcag cggaaaggtg attgacacgg ctgaccagcc cagcggcacg cagaaggcga
     7741 acaggaagaa cgggaagaag ctctcgcgca gccattggcg tccccacgcc aggcccatca
     7801 agccgtagat gccggcaaag agcgcgataa tggagatgcg ggtttgctgg gccaggtacc
     7861 cgaacaaatg cagcacgagc gccaaggcca ccagcagcag ccccggccac caggttttca
     7921 gcggcaaggc gagcaactgt ttgcgcttcc accagaagag cgccagcacg acgaacggta
     7981 tgaacttgcc atgcccgtcg tccgaaccgc tggcatcctc acccgaatag acagtgtgca
     8041 tccactctag cagcgacggg gtcgagatgt agcccagcgt cgaattgccc aggaattgaa
     8101 acagcgccag ccaggcgacc agcagaccaa ggaacaggcc cttgtttggc agccggttcc
     8161 agcattccat gaattggatg cgaaagtctt cgagaatgcc gttttccggt tgatttgcca
     8221 tgtgcacgtt gattcgaggc tattggatac tatattcgcc aatcctgctc aatcaattat
     8281 tcgggcgcgg cagcctcgcg cacgtagatc tcgaagcccg cgatagcggg aatag
//
LOCUS       DATCGS010000035         2973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_696556, whole genome shotgun sequence.
ACCESSION   DATCGS010000035 DATCGS010000000
VERSION     DATCGS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696556"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VIL39_00625"
     CDS             complement(<1..313)
                     /locus_tag="VIL39_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY5479960.1"
                     /translation="MLEYKNKILFVGYGAVARCTLPILVKHIKVPARDITVMDFEDRR
                     AVLAPWVKRGVKFVRQRVTRDNMDALLGKHLGAGDLLIDLAWNIDCCEVLQWCHDHGV
                     LY"
     gene            complement(403..1794)
                     /locus_tag="VIL39_00630"
     CDS             complement(403..1794)
                     /locus_tag="VIL39_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY5479961.1"
                     /translation="MHAPLAIEPSITTGRSGGGGGILVISAIVAALGSLLFGFDTAVI
                     SGTTDALRQVFTLNDNLLGFTVSSALIGTMVGSLLVGRPADWWGRRPVLAMLAVLFVV
                     SAVGCALAWNWYALLFFRWLGGVAVGGASVVCPMYITEIAPAKRRGLLVAVSQLNIVL
                     GILAAYFSNWLVARVLGADHPSTWRWMFGVMTVPAVAFLLTALMIPESPRWLVKRGRR
                     EQAETVLARFGHEDPAQQAGEIAESLKGEMSGVHQRLFQRKYLKPLLLACMIAAFNQL
                     DGINAVLYYASDIFHMAGADKASALMQSVIIGFTNLVMTILAMALIDRVGRKALLLVG
                     SVTFVMSHVLAAWVFATHAQGWIVMAALMGIVGTHAYSQGAVVWVIINELLPNAVRAS
                     GSAVVCCLVWVLCLGVSWSFPVVAAKPAGGAYAFGFFALMMVLQFVLVLKFLPETKGV
                     SLEELQKRLGVAD"
     gene            1966..2181
                     /locus_tag="VIL39_00635"
     CDS             1966..2181
                     /locus_tag="VIL39_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEY5479962.1"
                     /translation="MTIDTVHQLPRSEKLKLMETLWEELSRSDAGFESPAWHAKELAE
                     TEQRLAEGKEQVMDWEKAKKALRSKFE"
     gene            2178..2477
                     /locus_tag="VIL39_00640"
     CDS             2178..2477
                     /locus_tag="VIL39_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY5479963.1"
                     /translation="MRVRILTAALADLTSGRVFYERQGEGLGDYFFDSVFSDIDSLAL
                     YGGIHRKALGYHRLLARRFPYAVYYRMDTDGSVVVYRVLDCRQNPAKTAHELQTG"
     gene            2548..>2973
                     /locus_tag="VIL39_00645"
     CDS             2548..>2973
                     /locus_tag="VIL39_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5479964.1"
                     /translation="MEYVNLGSSGLKVSRICLGTMTYGSRKWREWVLDEPESRPLIRQ
                     AIEAGINFFDTADMYSVGVSEEILGRALKEFGPPRDRLVVATKVFNAMGEDPNQRGLS
                     RKHIHHAIDDSLRRLKMDYVDLYQIHRFDPSTPMEETLEA"
BASE COUNT          649 a          930 c          862 g          532 t
ORIGIN      
        1 tatacaacac gccgtggtcg tggcaccact ggaggacttc gcagcagtca atgttccacg
       61 ccaggtcaat cagcaggtcg cccgccccca ggtgtttgcc gagcagcgcg tccatgttgt
      121 cgcgcgtcac gcgctggcgc acaaacttaa cgccgcgctt cacccagggc gccagcaccg
      181 cccggcggtc ctcgaagtcc atgacggtaa tgtcccgggc gggcaccttg atatgcttga
      241 caagaatggg gagggtgcat cgcgccaccg ccccataacc gacgaataga atcttgttct
      301 tgtattcgag catagttatt gatcgaataa acgttcactg ccgtcaacca tcttcatcgg
      361 aaaacttcaa acattctatc acggcggagc ggttgctgga aatcaatccg ccacgccgag
      421 gcgtttctgg agctcttcca gcgaaacccc cttggtttcc ggcaggaact tcagcaccag
      481 cacgaattgc agcaccatca tcagcgcgaa gaacccaaac gcataggccc ctcccgccgg
      541 cttcgccgcc accaccggga aggaccaact cacgccaaga cagagcaccc agaccaggca
      601 gcagaccacg gccgagcccg aggcgcgcac cgcgttgggc agcaactcat tgatgatcac
      661 ccagaccacc gcgccctgcg aataggcatg agtgccgacg atgcccatca aggcggccat
      721 cacgatccag ccctgagcat gcgtggcgaa gacccaggcc gccagcacat gggacatgac
      781 gaacgtgacg gaaccgacca gcagcagggc cttgcgcccc acgcggtcaa tcagcgccat
      841 cgccagaatc gtcattacca ggttggtgaa gccaataatg accgattgca tcagcgcact
      901 ggccttgtcg gccccggcca tgtggaaaat atcggacgcg taatagagca ccgcattgat
      961 gccgtcgagc tggttgaagg cagcgatcat gcaggccagc agcagcggct taagatactt
     1021 gcgctggaac agccgctgat ggaccccgct catctcgcct ttcagcgatt ccgcaatctc
     1081 gcccgcctgc tgggccggat cttcatgccc aaaccgggcc agtaccgtct cggcttgttc
     1141 ccggcggcca cgcttgacca gccagcgcgg gctttccgga atcatcagcg ccgtcagcag
     1201 gaacgccacc gcggggacgg tcatgacgcc gaacatccag cgccaggtgc tgggatggtc
     1261 agcccccaat acgcgcgcca cgagccagtt cgagaaatac gccgccagga ttcccagcac
     1321 gatgttcagt tggctcaccg ccaccagcaa cccgcgccgt ttggccggag caatctcggt
     1381 gatatacatc gggcagacca ccgacgcccc acccaccgcc acgccgccca gccagcggaa
     1441 gaaaagcagc gcataccaat tccaagccag cgcgcagccc acggccgaaa cgacgaatag
     1501 caccgccagc atcgcgagca ccggccgccg gccccaccaa tccgccggtc gcccaaccaa
     1561 cagggagccg accattgtcc cgatcagcgc gctggatacg gtaaagccca ggaggttgtc
     1621 gttgagcgtg aacacctgcc gcaaggcgtc ggtggtgccg gaaatcacgg cggtatcgaa
     1681 gccgaagagc agcgacccga gggcggccac gatggcgctg attacaagga ttccgccgcc
     1741 gccgccgctg cggccggtgg tgatggaagg ttcaattgcg agtggagcat gcatgttcaa
     1801 ggctttggtt aatggccggc atcgggtggc gattctagcg ggcagggcca gacgatcaaa
     1861 cacttatgca ctcggatttc aggttttgcc gagtcggttc acttggaaga cattcaattg
     1921 gaagagggtt gccatcttct tatcccaacg ttaatttgag ctagcatgac cattgacacc
     1981 gtgcatcaat tgccccgaag cgagaaactg aagctgatgg agacgctgtg ggaagagctg
     2041 agccgctcgg acgcggggtt cgaatcgccc gcctggcacg ccaaggagct ggcagaaacc
     2101 gagcagcgtc tcgccgaagg caaggagcag gtaatggact gggaaaaggc caagaaggcg
     2161 cttcgcagca agtttgaatg agagtgcgga tactgaccgc ggctctcgct gatctgacct
     2221 ccgggcgtgt cttctacgaa aggcagggtg aaggcctggg agattatttc ttcgattctg
     2281 tcttctccga tatcgattcg cttgcgctgt acggcggcat tcatcggaag gcattaggct
     2341 accatcgctt gctggctcgc cgttttcctt acgcggttta ctaccggatg gacaccgatg
     2401 gttccgtagt cgtctatcgc gttctggatt gcaggcagaa tccagcaaag accgcacacg
     2461 aactgcaaac tggctagtgt cgggcaattc tcctcctgcg cgttcgaggg cttgaccgct
     2521 cgcatttctg ccctagcttg atgggctatg gaatacgtta atctcggttc gagcggactg
     2581 aaggtttcgc gcatctgcct gggcaccatg acctacggtt cgcggaaatg gcgcgaatgg
     2641 gtgttggacg agccggagag ccggccgctt atccgccagg ccatcgaggc cggcatcaac
     2701 ttctttgata cggcggacat gtactctgtc ggcgtcagcg aggaaattct cgggcgcgcc
     2761 ctcaaggagt tcgggccgcc gcgcgaccgg ctggtcgtgg ccaccaaggt cttcaacgcg
     2821 atgggtgagg acccaaacca gcgcggactt tcccgcaagc acatccatca tgccattgac
     2881 gatagcctcc ggcggctcaa gatggactac gttgaccttt accagatcca ccgctttgat
     2941 ccgtccacgc cgatggagga aaccctggaa gcg
//
LOCUS       DATCGS010000036         5472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_4110, whole genome shotgun sequence.
ACCESSION   DATCGS010000036 DATCGS010000000
VERSION     DATCGS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VIL39_00650"
     CDS             complement(<1..597)
                     /locus_tag="VIL39_00650"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY5479965.1"
                     /translation="MSKPQPDPKVAAARLKKLSRLRPTLAIVLGSGFHHVLSELRVNA
                     EVSYAKLPGFPPTGVSGHAGQLFIGHLGGTPVMVLSGRAHYYEGHPMTQVTFAVRALA
                     AYGIRDLLLTNAAGGLNRSFHPGDFMMLTDHINLMGVNPLRGAPVPGLPRFVDLTCAY
                     DKGLRGLLQAAGRACGTKLRAGTYLAVSGPSYETPAEIR"
     gene            647..1795
                     /gene="moeB"
                     /locus_tag="VIL39_00655"
     CDS             647..1795
                     /gene="moeB"
                     /locus_tag="VIL39_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY5479966.1"
                     /translation="MELTDAEIRRYSRHLILPEVGLEGQKKICSASVLCIGAGGLGSP
                     IAMYLAAAGIGRLGILDFDLVDLSNLQRQVIHGTLDVGRLKTHSAAETIHRLNPHVQV
                     VLHDTRLTNENALDIIRPYDIVVDGTDNFPTRYLTNDACVLLRKPNVYGSVFRFEGQA
                     SLFAPHLGGPCYRCLYPEPPPPGTVPSCAEGGVLGVLPGVIGLIQATETLKLVLGKGT
                     SLLNRLLLFNALDMQFRELKLRRDPQCPLCGEHPVITQLIDYQTFCGVPPPSTIPTMH
                     PDEVTVHELKRALDDPKLGIRVLDVREPDEYRIARINGARLVPLSTLPQRLTELDPDQ
                     PYYLHCKAGGRSMKALAFLRERGFKHLKSVKGGITAWSDEIDPTVPKY"
     gene            1875..1951
                     /locus_tag="VIL39_00660"
     tRNA            1875..1951
                     /locus_tag="VIL39_00660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1909..1911,aa:Arg,seq:ccg)
     gene            complement(2070..2906)
                     /locus_tag="VIL39_00665"
     CDS             complement(2070..2906)
                     /locus_tag="VIL39_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY5479967.1"
                     /translation="MRCYSHLILGAFVLGLAGVVVPCRASELAEKGREIFNQNQQAVV
                     TVQVVLKMSYSGAAKSSETRQEITGTVVNPSGLTVLALSAADPSEMYQRMMSEQGSQN
                     KLETEVTDLKILLADGTELPAEIVLRDKDLDLAFIRPKSKPATPMAAVDLSKSAPAQL
                     LDEVITLNRLNSAAGRAYSASVERISAVIQKPRTFYIPDSSMTATTLGSPAFALDGKV
                     VGLFVMRAVSSKSGGNRNMRDNMTSIILPAEDVLKGAKQAPEAKGDSEKKEEPKASTE
                     KK"
     gene            complement(2927..4984)
                     /locus_tag="VIL39_00670"
     CDS             complement(2927..4984)
                     /locus_tag="VIL39_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5479968.1"
                     /translation="MLGSNSCHILQRLALSFSLLFVSEAAQTGAAERSTPSALRSQID
                     SAIARVKPALVRIRVVSTDYSDGREVKMQAVGSGAIITRDGYLITNHHVAGHAARMFC
                     TMWNREEIEAELVGTDPLTDISILKLKPEHPVEFKYAEFGDSSKIQVGESVLAMGSPM
                     ALSQSVTLGIISNTEMTMPRFWGSQRRFRLDGEDVGSLVRWIGHDAAIYGGNSGGPLV
                     DLHGKIIGINEISYGLSGAIPGNLAQSVAKEIMAKGRVRRSWLGIDAQPLFKRSQDQH
                     GVLISGVLENSPADKAGFKPGDLLLSVGGAATDVRFEEQMADLMWRLASLTIGKEVTA
                     VVKRDGKEVSLRVVPIEREEAFPKEQEIRAWGLTVRDFSKLLAEEMKRPSLDGVLVTS
                     VRPGGPAGSAKPALQPSDVIVEVNHQPVKNTQDLIALTRKLTEGRTELVPAMATFERK
                     AARYLAVVRVGLEELKDPGLEVTKAWLPVETQVISREVAKQLGQPALKGFYITRVYTG
                     TTAEKAGLKPGDFITAVDGGKLSASDAEYEDELSSLIRQYDVGKTVELTVLREKAELK
                     VPVELARSPRLQREMRKYRSDDFEFTAREVSFFDAVEEQLASDQQGVLVESVKSGSWA
                     ELGSLSAGDLILEVDGRPVGNVDSLRRELERAAADKEKVVVIKVLRGIHTCYLEIEPN
                     WKN"
     gene            5110..>5472
                     /locus_tag="VIL39_00675"
     CDS             5110..>5472
                     /locus_tag="VIL39_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5479969.1"
                     /translation="MPDWIAVILLGIVEGITEFLPVSSTGHLLIFEHWLRIHQSDLFN
                     VVIQSGAVLAVIPLFHKRFHQFLFQWREKATQTYAAQIVLAVALTGGPGFVLEKKGFK
                     LPEQLAPVAWALLIGGIGF"
BASE COUNT          963 a         1766 c         1621 g         1122 t
ORIGIN      
        1 gcggatctca gccggggtct catagctcgg cccgctgacc gccaaatacg tgccggcccg
       61 aagcttcgtg ccgcacgccc ggccagccgc ctgcagcagg ccgcgcaatc ccttgtcgta
      121 ggcgcaagtc agatccacga accgcggcaa cccgggcacc ggcgcgcccc gcagcgggtt
      181 gacgcccatc aaattgatgt ggtcggtcaa catcatgaag tctcccgggt ggaagctgcg
      241 gttcaggccg cccgccgcgt ttgtcagcag caaatcccgg atgccatagg cggccagcgc
      301 gcgcactgcg aacgtcacct gtgtcatcgg gtgcccctcg taataatgcg cccggccact
      361 cagcaccatg accggggtgc ctcccaggtg gccgatgaac agttgccccg cgtggccgct
      421 tactcccgtt ggcggaaaac cgggcagctt cgcgtaagaa acctccgcgt tcactctcag
      481 ctcgctcaac acgtggtgaa agccgctgcc caagactatc gccagcgtcg gccgaagccg
      541 ggacagtttc ttcagccggg ccgcggcgac cttgggatcg ggttgtggtt tgctcatgct
      601 tgctctatgc tgcaattgaa ttagtttgcc cggtttccaa tttcctatgg aattaaccga
      661 tgccgaaatc cgccgttact cacggcattt gattctgccc gaagtgggcc ttgagggcca
      721 aaagaaaatt tgctccgcca gcgtgctttg catcggggcg gggggccttg gctcgcccat
      781 cgcgatgtac ctggccgccg ccggcatcgg ccggctgggc atcctcgact tcgacttagt
      841 ggatctctcg aatctccagc gtcaggtcat ccacggcacc ctggacgttg gccggctcaa
      901 gactcactct gccgcggaaa ccatccaccg tctcaacccg cacgtccaag tcgtcctgca
      961 cgacacccgc ctcaccaatg agaacgccct ggatatcatc cgcccttacg acatcgtcgt
     1021 ggacggcacg gacaactttc ccacccgtta cctcaccaac gacgcttgcg tgctgctccg
     1081 aaagccaaac gtgtatggct ccgttttccg ctttgagggc caggccagcc tcttcgcacc
     1141 gcacctgggc ggcccctgct atcgttgcct ttatcccgaa cccccgccgc ccggcaccgt
     1201 cccgagctgc gccgagggcg gcgtgctcgg cgtgctgccc ggcgtcatcg ggctcatcca
     1261 ggccaccgaa actctgaaac ttgtcctcgg caaaggcacc tcgctcctca accgcctgct
     1321 cctgttcaac gccctggaca tgcagttccg cgaactcaag ctgcggcgcg acccccaatg
     1381 ccccctttgc ggcgagcacc ctgttatcac ccagctcatt gactaccaaa ccttctgtgg
     1441 cgtcccgccg ccatccacca ttccaacgat gcatcccgac gaagtgaccg tgcatgaact
     1501 gaaacgcgcg ttggacgacc cgaagctcgg catccgggtc ctcgacgtgc gcgagccgga
     1561 cgaataccgc attgcccgca tcaacggcgc gcggctggtc ccgctcagca cgttgcccca
     1621 gcgactcacc gaattggacc cggaccagcc ctactacctc cactgcaagg ccggcggacg
     1681 ctccatgaaa gcgctggcgt ttctgcgcga gcggggcttc aaacacctca agagcgtcaa
     1741 aggcggcatc accgcctggt ctgacgaaat tgaccccacc gtgccgaagt actgagccgg
     1801 ctcattttcc atcgccgagg gcggcgaaaa caggttgccg gcagggaggg cttgatctac
     1861 agtcatctcg gtttgcgcga gtagctcaat tggatagagc gtcggcctcc gaagccgaag
     1921 gttgagagtt cgaccctctc ctcgcgcacc atctcccttt ggttttggac agagtatgtc
     1981 cagggtgccc ccagggcggc gctgatccgg gaggcaggtc tggcgaagga ggaattcgcc
     2041 gaaatgcttt gaacgccggc ggcgaagcgc tatttcttct ccgtgctcgc tttcggttct
     2101 tccttcttct ccgagtcgcc tttggcttcg ggggcctgct tcgcgccctt tagaacgtcc
     2161 tccgcgggga ggatgatcga ggtcatgttg tcgcgcatgt tacggttccc gccgctcttt
     2221 gagctaacgg cgcgcatgac gaacaggccg acgaccttcc cgtccagtgc aaatgcgggc
     2281 gagccgaggg tcgtcgcagt catggagctg tcagggatgt agaaggtccg gggtttttga
     2341 atgacggcgc taatgcgctc cactgaagcg gagtaagccc ggcccgcggc gctgttgagg
     2401 cggttgaggg tgatgacctc gtcgagcaac tgggcagggg cggatttgct caaatcaacc
     2461 gcagccatcg gcgtggcggg tttgctcttg ggccggatga acgccaggtc caggtccttg
     2521 tcccgcaaca caatctccgc cggcagttcc gtgccgtcgg ccagcaggat cttcaagtcc
     2581 gttacttccg tctccaactt gttctgggaa ccttgctcgg acatcatgcg ctggtacatc
     2641 tcgctgggat cagcggctga gagggcaagg acggtcagac ccgagggatt taccaccgtg
     2701 ccggtgatct cctgccgggt ctcgcttgac ttggcggcgc ccgagtagga cattttcagc
     2761 accacttgga cggttacgac cgcctgctgg ttctggttga agatttcccg gcccttctcg
     2821 gccagttcac tggcccggca gggaacgacg acgccggcca gcccgagtac aaaagcgcca
     2881 agaataaggt gtgagtagca acgcataaga gttctttgat tgaaggctag tttttccagt
     2941 tcggttcgat ttccaggtag caggtgtgaa tgccgcgcaa gactttgatc accaccacct
     3001 tctccttgtc ggcggcggcc cgttccagct cgcgccggag agagtccaca ttacccaccg
     3061 gccggccgtc cacttcgagg atcaggtcgc cggcagagag cgaaccgagt tcggcccaac
     3121 taccggattt gacgctttcg accagcaccc cttgctggtc gctggccagt tgttcttcga
     3181 ccgcgtcaaa gaacgacacc tcccgggccg taaactcaaa gtcgtcgctg cggtacttcc
     3241 gcatctcgcg ctggaggcgc ggcgagcggg cgagttcgac tggcaccttg agttcggcct
     3301 tctcgcggag gaccgtcaat tccaccgtct tgccgacgtc gtattgccgg atcaagctcg
     3361 agagttcatc ctcgtactcg gcgtcggagg ccgacagctt gccgccgtcc actgccgtga
     3421 tgaagtcacc gggtttgagt cctgccttct cggcggtggt gccggtgtaa acgcgggtga
     3481 tgtaaaaacc tttcaatgcg ggctggccga gttgtttcgc gacctcgcgg ctgatgacct
     3541 gcgtttcgac cggcagccag gccttggtga cttccaggcc ggggtccttg agttcctcga
     3601 ggccgacccg caccactgcg aggtagcgcg cggccttgcg ctcgaaggtc gccatcgccg
     3661 ggacgagttc cgtccggcct tccgtcagct tgcgggtcag ggcgatcaaa tcctgcgtat
     3721 tcttcaccgg ctggtggttg acctcgacaa tgacgtcgct gggctgcagg gcgggtttgg
     3781 cgctgccggc cgggcctccc gggcgaacgg acgtcaccag cactccgtcg agtgagggac
     3841 gcttcatttc ttcagccagc agtttcgaga agtcgcgcac ggtcagcccc cacgctctga
     3901 tctcctgctc cttagggaag gcctcctcgc gctcgatcgg caccacgcgg agcgagactt
     3961 cctttccgtc gcgctttacc accgccgtga cctccttgcc gatggtcagg ctggctagcc
     4021 gccacatgag gtccgccatc tgttcctcga agcggacatc ggtcgcggca ccgcccacgc
     4081 tcagcagcag gtcgccgggt ttgaagcccg ccttgtccgc cggagaattt tcgagcacgc
     4141 cgctgatcag caccccgtgc tggtcctggg aacgcttgaa aagcggctgc gcatcgatgc
     4201 ccagccagct ccgccggacg cggcctttgg ccatgatctc cttggccacg ctttgggcaa
     4261 ggttgccggg gatggcaccg ctcaggccgt aactgatctc gttgatacca atgatcttgc
     4321 catgcaaatc caccagcgga ccaccggagt tcccaccgta gatcgcggca tcgtgtccga
     4381 tccaacgcac tagcgagccg acatcctcac cgtccagccg gaaacgccgc tgggaacccc
     4441 agaaccgcgg catggtcatc tcggtgttgc tgatgatgcc aagcgtcacc gattgggaga
     4501 gggccatggg gctgcccatc gccagcacgg attcgcccac ctggattttc gacgaatcgc
     4561 caaactcggc gtacttgaac tcgaccgggt gctccggttt aagtttaagg attgaaatgt
     4621 ccgtgagggg atcggtgccc accagttcgg cctcgatttc ctcgcggttc cacatggtgc
     4681 agaacatgcg cgcggcgtgg ccggcgacgt ggtgattggt gatgaggtat ccgtcgcggg
     4741 tgataatagc cccgctgccg accgcctgca ttttgacttc gcgcccatcg ctgtagtcgg
     4801 tggacacaac acgaatccgc acgagggccg gtttgactct ggcgatggcc gaatcaattt
     4861 gtgatcgcag tgccgagggc gtgctccgct ccgccgcgcc ggtctgggcg gcttcggaaa
     4921 caaagagcag actgaaactg agagccagtc gctggagaat atgacacgaa ttgctgccga
     4981 gcatttgtgt ccttttagag caaatccgca cgcttgcaag cactgaatca agtgccggcc
     5041 gggtgacggg gccgggtaac gcggtatttg cggcttgtct ttcatggccg tttattgtct
     5101 aaacccctga tgccagactg gattgctgtt attctgctcg ggattgtgga aggcatcacc
     5161 gagttcctgc cggtttcttc caccggccac ttgctcattt tcgagcattg gctgcgcata
     5221 caccaaagcg acctgttcaa cgtcgtgatt caatccgggg cagtgctggc ggtgattccg
     5281 ctgtttcata agcggttcca ccagtttctc tttcagtggc gcgagaaggc cacccaaacc
     5341 tacgcggcgc aaatcgtgct cgccgtcgcg ctgaccggcg gaccgggctt tgtgctggag
     5401 aagaagggct tcaaactgcc tgagcaactg gcgccggtcg cctgggcttt gctgatcggc
     5461 ggcatcggct tt
//
LOCUS       DATCGS010000037         3386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_300877, whole genome shotgun sequence.
ACCESSION   DATCGS010000037 DATCGS010000000
VERSION     DATCGS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300877"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..709
                     /locus_tag="VIL39_00680"
     CDS             524..709
                     /locus_tag="VIL39_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479970.1"
                     /translation="MQNAHGSTVLDEMLEPVSRCFGLETARALVGLRANERTRTRVEA
                     LAGKCNEGQLTSEEQTE"
     gene            995..2509
                     /locus_tag="VIL39_00685"
     CDS             995..2509
                     /locus_tag="VIL39_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479971.1"
                     /translation="MQPHKYKAGLPLYPKAAAAKNNATVRIIGIRNLSCRPLKNFYRK
                     QRTLGITMKELTLKSLRLTSAGLSLSLCLLANHAGAAVQGWDPNGTTSIGGNGTWDTT
                     SLNWTPGGTQTQVASGSLVAFTSGNAALFCAGPSGSANQGTFTVTVSGTPTIGGIFNG
                     NLNPGPCNLTITGGGLNLATGTDAFSMYNNVTIINSPISGTTGGILNPQGSGSVSLFA
                     NNTYSGGTLLNSPTLVNFNNNNSFGTGVMTITRGSGLFAALLAQGGSTLTLANNFANT
                     TSGGGINFASAASTPVVSTGTWSLGANNLNLRNNGNSTSPLALSGAISGTANLTVSGA
                     NGGKIILSGANTYSGSTTVGSSAATSITLQAGTANEIASSSSLIMAGGTFNPGGFDQA
                     MASTTLNLTASSTIDFAAGASELKFANSSALTWTGILNLANWDSSLDLLRVGTDANGL
                     TLAQLGMIEFNGASLGSAQLNSLGFVVVVPEPSTALLGLLGGLSLMWTIRHRKV"
     gene            complement(2593..>3386)
                     /locus_tag="VIL39_00690"
     CDS             complement(2593..>3386)
                     /locus_tag="VIL39_00690"
                     /inference="COORDINATES: protein motif:HMM:NF045579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylhydrolase-like jelly roll fold
                     domain-containing protein"
                     /protein_id="HEY5479972.1"
                     /translation="DAGTIDWIHRKAGDTDIYFVASRWQPAEKVEGTFRVSGRQPELW
                     DPVTGAMRDAAAFHQQDGRTVVPLEFAPCGSTFVIFRKPIGANTAGTAKTNYPRAESQ
                     LTLSGTWEVSFDPKWGGPEKATFDQLVDWTTRPEPGIKFYSGTAIYRKEFILPTLPAK
                     GTRLLLDLGAVHEVASVRLNGRDLGVVWTKPARVDITDAVKAAGNALEVTVMNLWPNR
                     LIGDEALPKESQVTETNMRKFGSASPLLPSGLIGPVTVLSVREPQ"
BASE COUNT          690 a         1013 c          987 g          696 t
ORIGIN      
        1 cagcggcggt tggggcagcg gcggcaggaa ctgccggtca gcgatccgca acaaccgcgt
       61 cccgtcctac ggggacggcg gccttgggtt ccgggtggtc ttggccccag gtcagccatg
      121 agcagccagg cggagctgcc cggagtagtc gaggcgaagc gagcggagcg agagaagcct
      181 gactgcggag aggcagcgga ggtgcgttgg gcatggtaat gagctagagg gtgaaagacc
      241 cttgtggggc cttcggaagg caaccacgag gtcctgggct tctttgacat gtacttcctg
      301 gcgttgtcgc gggtgtcgga ccacttcgcg tatctgccat taaccgctct cgtggctctc
      361 gccgcggcgg ggctgggttg cggactgagc cgaatagttc acacgccaga agggctgact
      421 gacaacttgc ctgacaaaag ctccgccagg agcgccatgt ctatccactg cacggcggtg
      481 aaaccggggt tgcctttctc agccccgcat ttataatgtt ttcatgcaaa acgcacatgg
      541 cagcacggtc ttggacgaga tgctggagcc ggtcagccgt tgcttcgggt tggagacagc
      601 ccgcgcgctg gtcgggctgc gcgcgaatga gcggacgcgt acacgggtgg aagcgttggc
      661 cggaaaatgc aacgagggcc aactcacctc cgaggagcaa acagagtgac cgtgactgtc
      721 cgcacggtat caccggcaat gaactcagtg ccgccacagt tccgacatgc tttggatgga
      781 gtgctcataa tgtgatgatg cgtagtgctg cccggcgcca aagtgtgcga ccgccgccgg
      841 aaatgcaagg cagagcgtaa aggcaccacc gcgaccgcgg cgacgggctt ccagggtgcc
      901 agcagggagg cgtcagtccg gtgtccgttt agcgcgccgc tgaaccgata tccagtccag
      961 ccgctgcaat cgcacatttg attagaacat cttaatgcag ccgcacaaat ataaagctgg
     1021 attacccttg tatccaaaag ccgctgctgc taaaaataac gcgacggtac ggataatcgg
     1081 aataagaaac ctgagctgca ggccattaaa gaacttttat cgcaaacaaa gaactctcgg
     1141 cattaccatg aaagaactca cccttaaatc attaagactc acgtcggccg gcctatccct
     1201 ctcgctctgt ctcctggcga atcacgctgg ggcggctgtt cagggttggg atccaaacgg
     1261 gacaacctcg attggcggca acggaacttg ggacaccacc agcctgaatt ggacgccggg
     1321 aggaacccag acccaggtcg ccagcggctc cttggttgcc tttacgtccg gtaatgcagc
     1381 tcttttttgc gctgggccat caggttcggc caaccagggc accttcacgg tcaccgtatc
     1441 cggaacgcca actattggcg gcattttcaa tggaaactta aacccaggcc cttgcaacct
     1501 gaccatcacc ggtgggggat tgaatctagc caccggcacc gatgcgttca gcatgtacaa
     1561 caatgtcacc atcattaatt ccccgatttc cggtactacg gggggcatct tgaatcccca
     1621 aggctcgggc tcagtcagtc tcttcgccaa taatacttat tccggcggga ccttgcttaa
     1681 tagccccacg cttgtaaact ttaacaacaa caactcgttt ggcactggcg tcatgaccat
     1741 cacccgcggc tccggccttt ttgcagcgct gcttgctcaa ggcggttcga ctcttacgct
     1801 cgctaacaac tttgcaaata cgacttcggg cggcggtatc aattttgcca gtgcggcaag
     1861 tactcccgtg gtcagtaccg gcacttggag tttgggagcg aacaacctga acctgagaaa
     1921 caacggcaac agcacatccc cgctcgctct ctccggggca ataagcggca ccgccaatct
     1981 gaccgtaagc ggcgccaacg gcggcaagat cattttaagc ggtgcgaaca cttacagcgg
     2041 ttcgacgacc gttggttctt ccgcggccac ctccatcacg ctccaggccg gcaccgccaa
     2101 tgagatcgcc agcagcagca gcctgatcat ggctggtggc acgttcaacc cgggtggctt
     2161 cgatcaggcc atggcgtcca ccacgctgaa ccttacggct agctcgacga tcgattttgc
     2221 cgcaggcgca agcgaattga aatttgccaa cagctcggcg ctgacttgga ccggtattct
     2281 gaacctcgcc aattgggatt cgtctctgga cttgctgcgt gtcggcaccg acgcgaatgg
     2341 gctgaccctg gcccaactcg gcatgattga attcaatggc gccagccttg gaagcgcaca
     2401 gctcaactcc ctcggcttcg tcgtcgttgt tcctgaaccc tccacggccc tcctgggctt
     2461 gctgggcggg ctgagcctga tgtggacgat ccgccaccgg aaggtctgat cagcacgttc
     2521 ctctgataca gcaagggccg ccagcgatgg cggccctttc gctttccgcc cccgcaacat
     2581 tcgttttctc cctcattgcg gctctctcac ggacaggaca gtgaccggcc cgatcagtcc
     2641 ggacggcagc aggggcgagg ctgaaccgaa tttgcgcata ttagtctcgg ttacctgcga
     2701 ctctttcggc aaggcttcgt cgcctattag ccggttcggc cacaggttca tcacggttac
     2761 ttcgagcgcg ttccccgctg cctttacagc gtcggtgatg tccactcgcg cgggctttgt
     2821 ccagacgacg cctaaatcac ggccatttag ccgcaccgaa gcgacctcgt ggactgctcc
     2881 caagtcgagc agcagtcgtg tgcctttcgc cggcagagtc ggaaggatga attcctttcg
     2941 atagatcgcg gtgccagaat agaatttgat gccgggctcg ggccgtgtcg tccaatcaac
     3001 gagttgatcg aaggtggcct tctccgggcc gccccacttc gggtcgaagc ttacttccca
     3061 ggttcccgaa agtgtgagtt gtgattcggc gcgcgggtaa ttcgtctttg ccgtgccggc
     3121 agtgttggcg ccgatgggtt tgcggaagat gacgaaggtg gagccgcagg gggcgaattc
     3181 caggggaacg accgtgcgac cgtcctgttg gtgaaatgct gcggcgtcac gcatggcgcc
     3241 cgtgaccggg tcccataact ccggctgtct gccggatacg cggaacgtgc cctccacctt
     3301 ttctgcaggc tgccagcgac tggccacgaa gtagatatcg gtgtcgcctg ccttccgatg
     3361 aatccaatca attgtcccgg catcac
//
LOCUS       DATCGS010000038         3517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_696914, whole genome shotgun sequence.
ACCESSION   DATCGS010000038 DATCGS010000000
VERSION     DATCGS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696914"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VIL39_00695"
     CDS             <1..1341
                     /locus_tag="VIL39_00695"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479973.1"
                     /translation="RGDADFGIYWMDVAGHRELLVSDPRLPCSQPVPLAERPRPLVWA
                     NRVDYTQPIGTFYVQDIYAGPGLAGVSRGTIRKLRVVGLEFRAAGIGHNGSGGPAGGA
                     LASTPISIGNGAWDVKTVLGEAKVYEDGSALFTVPARKPVYFQALDERGCAVQTMRSW
                     STLQPGEYNACVGCHDNKNTAPPAREYGFSLAMKAGPQALAPFYGPPRGFSFPHEIQP
                     ILDRHCIRCHKDRQPIQALVQGEARQPILGKQRSVVSSGDGVKDQLAFSLLGEVTEDR
                     AAKRKWSDAYLVLTRAMADPADRAGESAPASTTQPSTLNPQLSSEEPFRGDCRGRVVN
                     WIGAQSVPEALPPNFAGAVRSELLTMLEKGHQGVKLSREELDKIACWIDLLVPYCGDY
                     REANAWTEEEMKKHERYAEKRRQMEEVEQQNIDAMLGTKEPARGPGPGRPMFMR"
     gene            1422..1919
                     /locus_tag="VIL39_00700"
     CDS             1422..1919
                     /locus_tag="VIL39_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5479974.1"
                     /translation="MSTVAEQLRQAREAKNLTEQQIVDITKIRVDHLRALEAGNYDVF
                     SAPVYIRGFVRGYAKLLHLDVPKIMAELEAELAQTTKFALPPPLTERPRGVLDLAMLQ
                     LSKVDWRMTLIGVGVVIVVVTVVSVWATWRHHDPLKGVKPGVYQSAPGSSGNTLPLPA
                     PAPRR"
     gene            complement(1994..2500)
                     /locus_tag="VIL39_00705"
     CDS             complement(1994..2500)
                     /locus_tag="VIL39_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY5479975.1"
                     /translation="MKLWIAQGFGVGRIPMAPGTFGSMVGVLWFGLLLMTGNLWLFIA
                     GTIAAIALSVWLCGAAEKTLGQTDPGSVVLDEIAAMPVCFLGWVAIPVCKTGALPGFA
                     DFFSAQTWPLTLGVFVAFRFFDVAKPWPVYQSQSLPGGWGVTIDDVLAAVYVNAVVLL
                     VYAGKTLL"
     gene            complement(2497..3174)
                     /gene="pgsA"
                     /locus_tag="VIL39_00710"
     CDS             complement(2497..3174)
                     /gene="pgsA"
                     /locus_tag="VIL39_00710"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY5479976.1"
                     /translation="MANGGGEITKTGKHDDKAILSPQFALDGMNLPNKLTVSRFILTI
                     AFLAVMFSRVKYHMSIALVLFVAAALSDYLDGEIARRRKLITNFGILMDPLADKIMVC
                     SAFIAFVGLNWIPAWMVVVIVARELAITGMRLLAVSRNVVLAAEGWGKYKTVSQIAAI
                     TALFVLACYNEWGPVGRAIFGFHFPSQTPWIEWVAELSKWLAVALTFISGWLYLWRNR
                     AIYLQDL"
     gene            3334..>3517
                     /locus_tag="VIL39_00715"
     CDS             3334..>3517
                     /locus_tag="VIL39_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY5479977.1"
                     /translation="MTTEQANERLTGDLKALMRDAEELMKATAGQAGEKLTEIRSRLA
                     AAIESAKTTCHRMEEKT"
BASE COUNT          719 a         1082 c         1128 g          588 t
ORIGIN      
        1 aggggcgacg cggactttgg catttattgg atggacgtcg cgggccatcg cgaactgctc
       61 gtctccgacc cgcgcctgcc atgcagccag ccggtgccgt tggcggagcg tccgcggcct
      121 ttggtgtggg ccaaccgagt ggattacacc cagcccatcg gcacgttcta tgtgcaggac
      181 atttatgccg gtcccgggct ggccggggtg tcgcgcggga cgatccggaa gctccgcgtg
      241 gtggggctcg agttccgcgc ggcggggatc ggccacaacg gcagcggtgg cccggctggc
      301 ggcgcgctcg ccagcacgcc catctctatc ggcaacggag cctgggacgt aaagaccgtc
      361 ctcggtgagg ccaaagtgta tgaggacggg tcggcgctgt tcactgtgcc ggcgcgcaag
      421 cccgtgtatt tccaggcgtt ggacgagcgc ggctgcgcgg tgcagacgat gcgcagttgg
      481 agcacgctgc aacccggtga gtacaatgcc tgcgttggtt gccacgacaa caagaatacc
      541 gccccgccgg cgcgcgaata cggtttcagc ctggcgatga aggcggggcc gcaagccctg
      601 gcgccattct atggcccgcc gcgcgggttc agctttccgc atgagataca acccattctc
      661 gaccgccatt gcatccgctg ccacaaagac cggcaaccca tccaggcgct cgtccagggc
      721 gaagctcgcc agccgatcct tggcaaacag cggtccgtcg tcagctcagg cgatggcgtg
      781 aaggaccagc ttgccttcag tctgctcggg gaggtgacag aggaccgcgc cgctaagcga
      841 aagtggagcg acgcctacct tgtccttacg cgggctatgg ctgaccctgc tgaccgtgcg
      901 ggcgaaagcg caccggcttc caccactcaa ccctcaacgc tcaaccctca actctcctcg
      961 gaggaaccgt tccgcggcga ttgccggggc agggtggtca actggattgg cgcgcaatcg
     1021 gtgccggagg cgctgccgcc gaacttcgcc ggcgcggtcc ggagcgagtt gttgacgatg
     1081 ctggaaaagg gccaccaggg cgtgaagctt tcgcgggagg agttggacaa gatcgcatgc
     1141 tggatagacc tgctggtgcc ttattgcggc gactaccgcg aggccaacgc ctggaccgag
     1201 gaggagatga agaagcacga gcgttacgcc gagaagcggc ggcagatgga ggaggtcgag
     1261 cagcagaaca ttgacgcgat gctcggcacc aaggagcccg cccgcggccc tggtcccggc
     1321 cggccgatgt tcatgcgctg atattggaca gacctctcgg aaccggcctt gcttttcagg
     1381 gattgccaag gccggcagga aagcgctaca acatgggggt catgtccaca gttgctgaac
     1441 aactccgcca ggctcgtgag gccaaaaacc tcacggagca gcagatcgtg gacattacca
     1501 agattcgcgt tgaccatctc cgcgcgctcg aagcgggcaa ttacgatgtg ttctctgcgc
     1561 cggtttacat tcggggcttc gtgcgcggct acgccaaatt gcttcacctg gacgtgccga
     1621 agatcatggc ggagctggag gctgagctgg cgcaaaccac caagttcgcc ttgccgccac
     1681 cgctgaccga acgcccgcgc ggcgtgctcg atctcgccat gctgcagctc tcgaaggtgg
     1741 attggcgaat gacgttgatc ggagtgggcg tcgtgattgt ggtggtaacc gttgtctcgg
     1801 tctgggccac ttggcggcat cacgatccgc tgaagggcgt gaagcccggg gtctatcagt
     1861 ccgcgcccgg ctcgtcgggg aacacgctgc cgttgcccgc gcccgcgccg cgccgctaag
     1921 ccatttatct caggttggcg gctgcgcctt cgcgacgggc agccagggaa ggtatgttca
     1981 gccgcccgct gagttacagg agcgtctttc ccgcatagac cagcagcacc accgcattca
     2041 cgtaaaccgc tgccagcacg tcatcaatgg tcacgcccca gccgccgggc aacgactggc
     2101 tctgatacac gggccagggc ttggcgacat cgaaaaagcg gaaggcgaca aacacgccca
     2161 acgtcagcgg ccaggtctgc gcggaaaaga agtcggcgaa ccctggcagc gcgccggtct
     2221 tacagaccgg aatggcgacc cagcccagga agcagacagg catcgcggca atttcatcca
     2281 gcaccaccga gcccgggtcc gtttgcccca gagttttctc cgcggcacca cacagccaga
     2341 ccgagagggc aatggcggca atggttccgg caatgaacag ccacaggttc cccgtcatca
     2401 gcaacagccc gaaccagagc acgccgacca tcgagccgaa cgtccccggc gccatgggaa
     2461 tacgccccac gccaaagcct tgagcgatcc agagtttcac aagtcctgca agtaaatggc
     2521 gcggttgcgc cagaggtaga gccacccgga gatgaaggtg agcgccaccg ccagccattt
     2581 ggagagttcg gccacccact cgatccaggg cgtctggctg ggaaagtgga agccgaagat
     2641 ggcgcggccc accgggcccc attcgttata gcaggccagg acgaaaaggg cggtgatggc
     2701 ggctatttgg gaaacggtct tgtatttgcc ccagccctcc gccgccagca cgacattcct
     2761 ggagacggcc agcaggcgca tcccggtaat ggccagctcg cgggcgacga ttaccacgac
     2821 catccacgcc ggaatccagt tcagcccgac aaaggcgatg aaggccgagc agaccatgat
     2881 cttgtccgcg agcgggtcca tcaggatgcc gaagttcgta atgagtttgc ggcgccgcgc
     2941 gatctcgccg tccagatagt cactcagcgc ggcggcgacg aacagcacga gcgcaattga
     3001 catgtgatac ttgactcgcg agaacatcac cgcgaggaag gcgatggtga gaatgaagcg
     3061 ggaaacggtc agcttgttgg gcaggttcat gccatcgagc gcaaactgcg ggctgaggat
     3121 tgctttgtca tcgtgtttgc ccgtctttgt aatctctccg ccgccatttg ccaatcagca
     3181 actcgcgtgg ccgcctcaat ttcgggctgc cggttcctgc tcccaggacc atgcttttac
     3241 cctctttgga gcagccgtaa cgatttgcta ttgctgtttg cgaactatgg gagatactat
     3301 gggtgtcgga gcaactcaaa gaaaaggaag aagatgacga cagaacaagc caacgaaaga
     3361 ctgacgggcg acctgaaggc gctcatgcgc gatgcggaag aactgatgaa ggccacggcc
     3421 gggcaggccg gggagaagct taccgagatc cgcagccggc tggcggcggc gatcgagtcc
     3481 gccaaaacca cctgccaccg gatggaagaa aagacca
//
LOCUS       DATCGS010000039         4826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_300902, whole genome shotgun sequence.
ACCESSION   DATCGS010000039 DATCGS010000000
VERSION     DATCGS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300902"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VIL39_00720"
     CDS             <1..609
                     /locus_tag="VIL39_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5479978.1"
                     /translation="RLTRWPEHQRARLIGRVFQNPFTGTAPSMSIAENFALAARRGLG
                     RGLGWALAPKLMAELRDRVSQLKMGLEDRLDNAIGSLSGGQRQALTLLMASWINPALL
                     LLDEHTAALDPKSADQVIRVTEEVIKRHKLTTLMVTHSMQQAVNLGDRIIMMHQGAVA
                     YCFEGDEKQRLRVNDLLARFEELRRREMLDETAATLLEKTYV"
     gene            606..1034
                     /locus_tag="VIL39_00725"
     CDS             606..1034
                     /locus_tag="VIL39_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5479979.1"
                     /translation="MSEISPSPAHSLLEITVEHQQDNRVRVALKGRLDTHTYLQCEQR
                     LAPLLTTPKRVLVFDMAQLDYMSSMGLRVVMKTSKALAAIGGKCLLTRLQPSIRTVID
                     IANALPSENIFASVEEADRYFDVMQRRVKETGSANPPLVP"
     gene            1225..1701
                     /locus_tag="VIL39_00730"
     CDS             1225..1701
                     /locus_tag="VIL39_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479980.1"
                     /translation="MIDQKHCPFCRTSAAAGCKHLAVAAEGRDFVRRCVEMCQGEKPW
                     RALGERQRRQRLATGKSSPEREDYTWIETAFCEQFLKRLAWFGGIEYELRSGPKPNQA
                     GFWVLLWSKEPRQLWWELRDEFERQAHEGQPKRRSGPRAAVRSPKPDRWNEIALPL"
     gene            1744..2814
                     /locus_tag="VIL39_00735"
     CDS             1744..2814
                     /locus_tag="VIL39_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5479981.1"
                     /translation="MIRNIIFDWSGTLVDDLPAVWEATNYVLAQSQRPEMSLEQFRAE
                     FCLPFTIFYDRHVPHIPLPQLETWFHSRFREVQGSVCALPHAREFLEFCRARKLRTFL
                     LSTVNRDHFAVQSASTGFGPFLDKLYVNVWDKREKIHEILEENSLAPDETLFIGDMQH
                     DIETARHGGIHSCAVLTGYNTLEQLRLAEPDLIVEHLAELREVLEQNDLHLKPQGKRF
                     EEGHPPIVTVGGLIFNGTGQVLMVRTHKWSNLWGIPGGKVKWGEPSVEALRRELQEET
                     NLAVTDIEFVLVQDCIHSKEFYRDAHFVLLNYTCRCAGEPAVKLNDEAREFRWVTVAQ
                     ALELPLNQPTCKLLLAVAASKR"
     gene            2825..3181
                     /locus_tag="VIL39_00740"
     CDS             2825..3181
                     /locus_tag="VIL39_00740"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY5479982.1"
                     /translation="MSKITIVDLEVAYCVGVTEEERATPQRLLVTVDMSFDFTSAALS
                     DRIERTINYQLVAEDLLKFGEGRSWNLLEKLANNVADWVLTQYKPETVLVEVKKFSIP
                     QARFVSVSMGKTRVTR"
     gene            complement(3234..3680)
                     /locus_tag="VIL39_00745"
     CDS             complement(3234..3680)
                     /locus_tag="VIL39_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5479983.1"
                     /translation="MNPNGIISEILSHKGSTAWSISPDATVFEAIQVMADKNVGALLV
                     TENNKLVGIISERDYTRKVIVKGKSSKTIPVREILSSQVIHVSPSHTVEDCLRLMTDH
                     HFRHLPVLDGDKIAGIVSIGDLVNWIISAQHSTISQLETYITGVPG"
     gene            complement(3735..4511)
                     /locus_tag="VIL39_00750"
     CDS             complement(3735..4511)
                     /locus_tag="VIL39_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5479984.1"
                     /translation="MSVTEILGLMLAAAAGGAINAVAGGGTLVTFPALLFFGTPPIIA
                     NATSTLALVIGTSGSIYGYRRHLEPVRPWLWRFVPVSMVGGLMGAVLLTCTSNKTFSK
                     LVPFLILFATVLFVAQGVFRRFAAGDGQAAHAGKHGAVWGAILFQLAVAIYGGYFGAG
                     IGILMLASLGFIGLSNIHEMNALKTILGSLINLVAAVWFIGAGLIHWPKAGVMTAGAL
                     LGYFLGSHYAQRIPQQRVRQIITLIGFVISAVTFYEEFLR"
BASE COUNT          982 a         1470 c         1495 g          879 t
ORIGIN      
        1 cggctgacgc gctggccgga gcaccaacgc gcgcggttga ttggccgggt ctttcagaac
       61 ccgttcacgg gcaccgctcc cagcatgtcc atcgcggaga atttcgcgct cgcggcccgg
      121 cgcggcctgg ggcgcggcct gggctgggcg ctcgccccga aactcatggc cgagctgcgc
      181 gaccgtgtca gccagcttaa gatggggttg gaagaccgtc tcgacaacgc catcggcagc
      241 ctctccggcg ggcagcgcca ggccctcacg ctgcttatgg cctcctggat caacccggcg
      301 ctgctgctct tggacgagca caccgccgcc ctcgacccga aaagcgccga tcaagtcatt
      361 cgcgtcaccg aggaagtcat caagcgccac aagctgacca cgctgatggt cacccactcc
      421 atgcaacagg cagtgaatct cggcgaccgc atcatcatga tgcaccaggg cgcggtggcc
      481 tactgcttcg aaggcgatga aaagcagcgc ctgcgcgtga atgatctgct ggcaaggttc
      541 gaggagcttc gacgacggga aatgcttgac gaaacggcgg caaccttgct agagaagacc
      601 tacgtatgag cgaaatctct ccatctccag ctcacagcct gctggaaatc acagtcgaac
      661 atcagcagga caatcgggtc cgggtagcct tgaagggccg tctggacaca cacacttatc
      721 tgcagtgcga acaacgcctg gcgccgttgc tgaccacccc caagcgcgtg cttgtcttcg
      781 atatggccca actcgactac atgagcagca tgggcctgcg agtggtgatg aagaccagca
      841 aggcccttgc agcgatcggt ggcaaatgcc tgcttacgcg cctgcaaccc tcgatccgca
      901 ccgtcattga cattgccaac gccctgcctt cagagaatat tttcgcgagc gtcgaggaag
      961 ccgaccgcta tttcgacgtg atgcagcgcc gcgtgaaaga gacaggctcc gcgaacccgc
     1021 ccctcgtgcc gtaagggaat gtaaatatcc attctgcttt tcaagctgag gcttgtgaga
     1081 tgcctccttg aaaacaggag gcatgatggc ctcctggcgt gttcctaact cggctccgct
     1141 tccgggaaat ggaagccata gccaaaccaa cctgctgata atgtgagcgt gcgttggttt
     1201 cctgtgtttg aatataatct gaacatgatt gatcaaaagc attgcccgtt ttgcaggacc
     1261 tccgccgctg cggggtgcaa gcacctggcg gtggcggcgg aagggcgcga ttttgtgcgg
     1321 cgttgcgttg agatgtgcca gggagagaag ccgtggcgcg ccttgggcga gcggcagcgc
     1381 cggcagcggc ttgctaccgg caagtcgtcg ccggaacggg aagactacac ctggatcgag
     1441 acggccttct gcgagcagtt tcttaagcgg ctcgcctggt tcgggggaat tgaatacgaa
     1501 ttgcggtccg ggcccaagcc caaccaagcg ggtttctggg tattgctgtg gtcgaaagaa
     1561 ccgcggcagc tctggtggga attgcgcgac gaattcgagc gacaggccca cgaaggacag
     1621 ccgaagcgac gcagcggtcc gagggctgcc gtccggagcc cgaagcctga caggtggaac
     1681 gaaatcgcgc tgccgctgtg aagcaggtat tgccggaatg agaaaggccg ctacactcgg
     1741 cccgtgattc ggaacattat cttcgactgg tcgggcacgc tcgtggatga cctgcccgcc
     1801 gtctgggagg cgacaaacta cgtcctggct cagtcccagc ggccggagat gagcctggaa
     1861 cagtttcgcg ccgagttctg cctgcctttt acgatcttct acgaccggca cgtgccgcac
     1921 attcccctcc cgcaattgga gacctggttc cacagccggt tccgcgaggt gcagggctcg
     1981 gtctgcgcgc tgccccacgc gcgcgagttc ctcgagttct gccgcgcccg caagctccgc
     2041 accttcctgc tgagcaccgt caatcgcgac catttcgccg tgcaatccgc cagcaccgga
     2101 tttggcccgt tcctcgacaa gctgtatgtg aatgtctggg acaagcggga gaaaatccac
     2161 gaaatcctgg aggagaactc tctggccccg gacgagacgc tgttcattgg ggacatgcag
     2221 cacgacatcg agacggcccg gcacggaggc atccactcct gcgcggtgct cacgggctac
     2281 aacacgctcg aacaactgcg cctggcggag ccggacttga tcgtcgagca cctggccgag
     2341 ctgcgggaag tactggagca gaatgacctg cacctcaaac cccagggcaa acgattcgag
     2401 gagggccatc cgcccatcgt gaccgtcggc gggttgatct tcaacggcac gggccaggtg
     2461 ctgatggttc gcacacacaa gtggtcgaat ctctggggca ttccgggcgg caaggtcaag
     2521 tggggcgagc cttccgtcga ggcgttgcgg cgcgaactcc aggaggagac aaacctggcg
     2581 gtcacggaca ttgagttcgt gctcgtgcag gattgcattc actcgaaaga attctatcgc
     2641 gatgcgcact tcgttttgct gaactacacc tgccggtgcg cgggcgagcc ggcagttaag
     2701 ctgaatgatg aggcccgcga gtttcgctgg gtgaccgtgg cgcaagccct cgaactgccg
     2761 ctcaaccagc ccacgtgcaa gctgttgctg gccgtggcag cgtcaaaacg ataggccaaa
     2821 gagcatgtcc aaaatcacga ttgtggatct ggaagtcgcc tactgtgtcg gtgtcaccga
     2881 agaggagcgc gccacgccgc agcgcctcct ggtgacggtg gatatgagtt tcgatttcac
     2941 atccgccgcg ctaagcgacc gcatcgaacg gacaatcaat taccagttgg tggcggaaga
     3001 cttgctgaag ttcggtgagg gccggagttg gaatctcctc gagaaactgg ctaacaacgt
     3061 ggcagactgg gttctgacgc aatacaagcc cgagacggtg ttggtggagg tgaagaagtt
     3121 ctccattcca caggcccgct tcgtctcggt cagcatgggc aagacccgcg tgacgcgcta
     3181 acgcacggtc ctggcctcgc catgcgcggc aacgggcttc cgctgcctgc ggctcagccc
     3241 gggacgccgg taatgtaagt ctccaactgg ctgatcgtcg agtgctgcgc cgagatgatc
     3301 cagttcacca ggtccccgat cgagacgatg cccgcgatct tgtccccgtc cagcacgggc
     3361 aggtgccgga agtggtggtc ggtcatcagc cgcaggcaat cctccacggt gtgtgatggg
     3421 ctcacatgga tgacctggct ggaaaggatt tcccgcaccg gtatggtctt cgacgacttg
     3481 cccttcacga tcaccttgcg cgtgtagtcg cgctccgaaa tgatgcccac cagcttgttg
     3541 ttctccgtca ccagcagcgc cccgacgttc ttgtcggcca tcacttgaat ggcctcgaac
     3601 acggtggcgt ccggggagat ggaccaggcg gtgctgccct tatggcttag gatttcgctg
     3661 atgattccat ttgggttcat ggtggctaca ccttactacg gccgggcccg cttgccacca
     3721 actttcttgc gcgttcagcg caggaactct tcgtagaagg tcactgccga gatgacgaag
     3781 ccgatgaggg tgatgatctg ccgcacccgc tgctgcggaa tgcgctgggc gtaatgcgag
     3841 cccaggaagt agccgagcag cgcgccggcc gtcatcaccc cggctttggg ccagtgaatg
     3901 aggcccgcgc caatgaacca gactgcggcc acgagattga tcagggagcc gagaatggtc
     3961 ttgagggcgt tcatctcatg gatgttgctc aggccaatga aacccagcga ggccagcatc
     4021 aggatgccga tgccggcgcc gaaatagccc ccgtaaatgg ccacggcgag ctggaacaga
     4081 atcgcgcccc aaaccgcgcc atgcttcccg gcatgagcgg cctgcccatc cccggcggcg
     4141 aagcgccgga agacgccctg ggccacgaag agcaccgtcg caaacaggat caggaacggc
     4201 accagcttgg agaaggtctt gttgctggtg caggtcagca ggacggcgcc catcaaacca
     4261 cccaccatac tgacgggcac aaagcgccac aaccagggcc ggaccggttc gagatgccgg
     4321 cggtaaccgt aaatgctgcc cgaggttccg atgaccagcg ccagcgtgct ggtggcgttg
     4381 gcgataatcg gcggtgtgcc aaagaacagc agcgccggga acgtcaccaa tgtcccgccg
     4441 ccggccacgg cattgatcgc gccgcccgcc gccgcggcca gcatcagccc caggatttca
     4501 gtcaccgaca ttattagcgc ggcagcatag ccgaggatga gtggaactca agcccgaccc
     4561 gtggcgcaca ggggctcaac gccgggcggt tgccaaagtc gccgggatgt cccggcgcca
     4621 cggatgtgga gtgcggcggc aagcgaagcg cgacggccct cgggcgcagt cggcttcgac
     4681 ccgcgcagcc gtagggcact actgccaaga ccgttggata gattacaatg caatgcacga
     4741 accacttgat tttactgggt atcagtcatc tatagtgtgg tctatccaac ggtcttggca
     4801 gtatggggtc ccgaacatcc aacttc
//
LOCUS       DATCGS010000040         4469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_201890, whole genome shotgun sequence.
ACCESSION   DATCGS010000040 DATCGS010000000
VERSION     DATCGS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201890"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VIL39_00755"
     CDS             complement(<1..885)
                     /locus_tag="VIL39_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEY5479985.1"
                     /translation="MKRESIFTAMLLVFLSPGIIARAQIPAPPPAPEVQLRSGAELDQ
                     LLGPIALYPDPLMAQILPAATLPSEIVLADRYVNGGGDPNFIDQQPWDPSVKALARYP
                     TILKWMDDNLAWTTALGQAFLYQQQDVMASIQRLRAQAQALGNLQTTPQENVIIDNGI
                     IEIVPANPQVIYVPVYQPDVVYFQRPFGNPFISFGLGFSVGAWLNFDFDWGNHHLICW
                     GRDQPRPANWWYRRPGERPRVELAHATVWQPRNHPGAAAQGFDRGWDSRPVRSTVTVT
                     GGQPRPAEAPGSRPASPAP"
     gene            1089..3521
                     /locus_tag="VIL39_00760"
     CDS             1089..3521
                     /locus_tag="VIL39_00760"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5479986.1"
                     /translation="MQRSELNLRAMKDGRRPVRYWVLVMGAALLLCGGARFVAAAEAS
                     SEDTSKSVTAAAAGTNVAAIPLAEVAAQAEAAFASLQSIEANLSADDATVAIQQDLPG
                     LTRESSARLEESSKTLSSSPSLQTLSRLNREWQGVRDELADWKRSLTKRVAQLDEDRG
                     RLAQLEQVWKATQASVLESKLPPEILEQMERVMAAVKRTRDKAEAQQMVLLSLQTRVA
                     EQDGRAIHTLNAGERAREAFIRRLFVRDGPPVWSAELWSDSAQNLVVQSHHSLSRQVQ
                     VLREYVERKSGGFLLQGVLFLAILFGLVEVRQTLRRLGGDEASHAARVFDSPVATALV
                     LVLLTSSWVYPEAPRLFLAIVGAAAMIPTVLVVRKLIDRRLFPVLNALVIFYFVDQLR
                     VVAASQAVLSRLLVLAEMLGGVLFVGWLLGLRRLGAAPEDNRFWKAIGKACWVALDWF
                     AAAFLADVLGYVSLSKLLSHALLQSAYMALLLYAVARIIDGLFLSALSVPPLARLGLV
                     KRHQDLLRRRARGVLTWTAFLLWAVYVLEALSLRAPLFQNLHEVLTASLGVGSFKVSL
                     GDVVLFGITVWASFLVSRIVRFVLEEEVYARVKLAPGLHYSISKMVNYVILVIGFFIG
                     VAVLGFDLTRLTILVGAFGVGLGFGLQNIINNFVSGLILLFERPIKVGDVVQLGANEG
                     VVKHIGIRASIVEAPNGSEFIVPNAGLISETVTNWTLSDRLRRIDLPVAVAGPADARR
                     VMELIKAAAVGQPWVLKDPPPQTLLVSFAGDSMNFELRIWTNQSEDWSQIRSELALAV
                     RATLAEHSIAMK"
     gene            complement(3569..>4469)
                     /locus_tag="VIL39_00765"
     CDS             complement(3569..>4469)
                     /locus_tag="VIL39_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5479987.1"
                     /translation="RYPGPIVFEGNAPADVRENALLRVLLEAPTVKPTTVGRIWLGAP
                     NSIKGPTEAAFQRQSGNNLLLVGQRDEAMLSMLSVGLVSLAAQYPLGTARFILWDGTA
                     PGTSQREYIDRIVRSIPHPITQAKPGDLGEIMKELGQEMKQRSEAADAEAAPPVFLFI
                     HGLQKYSKLRYEEDFGFSSGDAEAEPNPAMVLNNLVCEGTRLGFHVIATCDTYNNVNR
                     YLSRKAFSEFEMRVLFQMSANDSASLIDNPKASLLGLHRALFYNAQEGYLETFRPYAL
                     PGSEWVEQTARNLARLLKGNTPG"
BASE COUNT          751 a         1318 c         1462 g          938 t
ORIGIN      
        1 cggtgcgggg ctggctggtc gcgagccggg cgcttccgcc ggccgaggtt ggcctccggt
       61 tacagttacc gtgctgcgga ccgggcgaga gtcccagccc cggtcgaagc cctgagcggc
      121 cgcaccggga tggttgcgcg gctgccagac ggtggcgtgg gccagctcta cccgaggacg
      181 ttcgccaggg cgacgatacc accaattcgc cggacgcggc tggtcacggc cccagcagat
      241 gaggtggtga ttgccccaat caaagtcgaa gttgagccat gcgcccacgg aaaagcccag
      301 gccgaaagag atgaacgggt tgccgaaggg tcgctggaaa tagaccacat caggctgata
      361 tacgggcaca tagatcacct gcggattggc cggcacgatt tcgatgattc cgttgtcgat
      421 aataacattc tcctgcgggg tggtttgcag gttccccaga gcttgggcct gggcacgaag
      481 ccgttgaatg gaagccatca cgtcctgctg ctgataaaga aacgcctgcc ccaaggctgt
      541 tgtccaggcc aagttatcgt ccatccattt gagaatggtc gggtatctgg ccagcgcctt
      601 gacgctggga tcccagggtt gctggtcaat gaagttcggg tcaccgccgc cgttcacgta
      661 ccgatccgct aagacgattt ctgagggcaa cgttgcggcc ggcaggattt gcgccatcaa
      721 cgggtccggg taaagcgcaa tcgggcccag cagttggtcg agttcggcgc ccgagcgcag
      781 ttgcacttcc ggcgccggag gcggagctgg gatttgggcc cgcgcgatga tccccgggct
      841 gaggaagacc aagagcatgg ccgtgaaaat ggattctcgt ttcattcgct tctccgggtt
      901 attcttgttc ccatcttcca gatcttactc cctttcacac ctgctgccag tggaatctga
      961 tgatcacccg ttcgccttat ccctgatccc tcccgtctca accctattac tccttgcttc
     1021 atcacattcc gcgcgtcctt gctggcggcg tggtgtctga gtattcttgt aaccgttaga
     1081 gttttgctat gcaacgatcc gaactgaact tgcgggcgat gaaagatggg cggcgcccgg
     1141 tgagatactg ggtgctggtg atgggtgccg cgctgctgct ttgcggtggc gcgcggttcg
     1201 tggctgcggc ggaggcgagt tcagaggaca ccagcaagag tgttaccgcg gcagccgctg
     1261 gcacgaacgt ggcggcgata ccgctggcgg aggtggcggc gcaggccgaa gcggcctttg
     1321 ccagcctcca gtccatcgaa gccaatttgt ccgccgacga tgctaccgtg gcgattcagc
     1381 aggacctgcc tgggctgacc cgggagtcga gtgcgcgcct ggaagagagc tcgaagacgc
     1441 tttcgtccag tccctcgctg caaacgctga gccggttgaa ccgggagtgg caaggggttc
     1501 gcgacgagtt ggcggactgg aaacgcagct tgacgaagcg cgttgcgcaa ttggacgaag
     1561 accgggggcg actggctcaa ctggagcagg tctggaaggc cacccaggct tccgtgctgg
     1621 agtcgaagct gccgccggag atcctggaac aaatggagcg agtcatggcc gcggtcaagc
     1681 gcacccggga caaggcggag gcgcaacaaa tggtgctgtt gagcctgcaa acccgggtgg
     1741 cagaacagga tggccgggcg atccacaccc tcaatgccgg cgagcgcgcg cgcgaagcgt
     1801 tcatccgccg tttgtttgtg cgggacggcc cgccggtctg gagcgcggaa ctgtggtcgg
     1861 actcggcgca gaatctcgtg gtgcaaagcc atcattcgtt gtcgcgccag gtgcaggttc
     1921 tgcgcgaata cgtcgagcgg aaaagcggcg gctttcttct gcaaggggtc ctgttcctgg
     1981 cgatcttgtt cggcctggtc gaagtgcgcc aaaccttgcg gaggctgggt ggagacgagg
     2041 cgagtcacgc cgcgcgagtg tttgattccc cggtcgcgac ggcgctggtg ctcgttctgc
     2101 tgacgagtag ttgggtttac cccgaggcgc cacggttgtt cctggccatt gtaggcgccg
     2161 cggcgatgat cccgaccgtc ctggtggtgc gcaagctcat tgaccggcgc ctgtttcccg
     2221 tcctgaacgc attggtgatt ttctactttg tagatcaact gcgggtggtc gccgcctccc
     2281 aggcggtctt gtcccgattg ctggttttgg cggagatgct gggcggcgtt ttgtttgtag
     2341 gctggctgct ggggctgcgc cggctggggg ccgcgccgga ggacaaccgc ttctggaagg
     2401 ccattggcaa agcctgctgg gtggcgttgg actggtttgc ggcggccttt ctcgccgacg
     2461 tcctgggcta tgtaagcctg agcaaactcc tcagccacgc tctgctgcaa agcgcctaca
     2521 tggccctgct cttatacgcc gtggcgcgga ttattgatgg actcttcttg agcgcgctca
     2581 gtgttccacc cctggcgcgg ctgggtttgg tgaagcggca tcaggacttg ctgcgcaggc
     2641 gggcgcgggg cgtgctgaca tggacggcct ttctgctctg ggcggtctat gtgctggagg
     2701 cgctgtcatt gcgcgcgccg ctgttccaaa acctccacga ggtgctgacg gcctccttgg
     2761 gggtgggctc gttcaaagtc tccctgggcg atgtggtgtt gttcgggatc acggtctggg
     2821 cgtcgtttct cgtctcgcgg atagtgcgtt ttgtcctgga ggaggaggtg tatgcccggg
     2881 tgaaactggc gcccggactt cactattcca tctccaagat ggtcaactac gtcatcttgg
     2941 tcatcggctt tttcatcggc gtcgccgtgc tgggattcga cctgacgcgc ctgacgattc
     3001 tggtgggcgc cttcggcgtg ggcttgggct tcgggctgca gaacatcatc aacaactttg
     3061 tctccggcct tattctgctg ttcgagcggc cgatcaaagt cggcgatgtc gtccaattgg
     3121 gtgccaacga aggcgtggtc aaacatatcg gcatccgcgc cagcattgtg gaggctccaa
     3181 acggttcgga gttcatcgtg cccaacgccg gtttgatttc cgagacggtg accaactgga
     3241 ccctctcgga ccgtctgcgc cggattgacc tgcccgtcgc ggtagctggc ccggcggatg
     3301 cccgccgggt catggagttg atcaaagccg cggcggtcgg gcaaccgtgg gtgctcaagg
     3361 acccgccgcc ccagaccttg ctcgtcagct ttgccggcga ctcgatgaat ttcgagctgc
     3421 gcatctggac caaccaatct gaggattggt cccagatacg gagtgagctg gccctggctg
     3481 tccgcgctac cctggccgaa cacagtattg caatgaaata accggctact gctccaaacg
     3541 cttcgcgcag ttcggttgcg gctttgcgtt atccgggagt attacccttg agcagccgcg
     3601 ccagattccg ggcggtctgc tccacccatt cgctgccggg cagcgcgtag gggcggaagg
     3661 tttcgaggta gccttcctga gcgttgtaga agagcgcgcg atgcagcccg agcaggctgg
     3721 ccttcgggtt gtcaatcagg ctggccgagt cgttggcgct catttggaag agcacgcgca
     3781 tctcgaattc actaaaggct ttgcggctga ggtaacgatt gacgttgttg taggtgtcgc
     3841 aggtggcgat cacatggaac ccgagccgcg tcccttcaca aacgaggttg ttcagcacca
     3901 tggccggatt gggttcggcc tcggcgtccc cactggagaa gccaaaatcc tcttcatagc
     3961 gcagcttgct gtatttctgc aagccgtgga tgaacaggaa caccggcggt gctgcctcgg
     4021 catccgctgc ctccgaccgc tgcttcatct cctgcccgag ctccttcatg atttccccca
     4081 agtcgcccgg tttcgcctgt gtgatggggt gcgggatgga ccggacgatc cggtctatat
     4141 actcgcgctg ggaggtgccg ggcgccgtgc catcccacag gatgaagcgc gcggtcccca
     4201 gcgggtactg ggcggccagg gacaccagtc cgacggacag catagacagc atcgcctcgt
     4261 cgcgctgccc caccaggagc aggttgttcc cactctgccg ctggaacgcc gcctccgtcg
     4321 gccccttaat ggaattgggc gcgcccagcc aaatgcgccc gactgtcgtc ggcttgactg
     4381 tgggcgcctc caacaaaacc cgaagcagcg cattctcccg cacgtccgcc ggcgcgttcc
     4441 cctcaaacac aatcggcccc ggatagcgc
//
LOCUS       DATCGS010000041         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_103256, whole genome shotgun sequence.
ACCESSION   DATCGS010000041 DATCGS010000000
VERSION     DATCGS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103256"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VIL39_00770"
     CDS             complement(<1..1617)
                     /locus_tag="VIL39_00770"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY5479988.1"
                     /translation="MPSTALTALLCVTTMVLFSAMTPALAAESEAHSSALGFAAVEQQ
                     FRELPLEARRLTGPLFWLHGDDSKERLEMYVGKVAEGGNGAFTTESRPHTDWMGPGWW
                     RDLDICLAAAKKQNLQLWIFDEKWWPSQAVAGKVPPRYAAKRLEAAAVELEGPRAFEA
                     DGYEGDKYVAAVAGQVNAEGKMDGNTLVDLGPNIRGGKLRWQAPAGKWRILKFTHKQA
                     PGLAQVGRSQLTVDGASRDCVDWFLKKVYQPHFEHYRADFGKTIRGFFYDEPETPGDW
                     GTELNPVLAEWNVDWKKAYVAYKSELAGEDQVAARYQYLDAFAEAWGRTMYGGMAEWC
                     HQHGVKSIGHFMEHANLYQNREFCAGDMMRLQGHGDMGGIDAVFEQFVMGKRVTRDAP
                     CWQTPKLASSISHAYGKPDDVAMVEIFGARGQNLTYPEMKWWADHMQVSGVNFLIPHS
                     FNPRSPHDTDCPPYFYDGGYEPRWPLYRVFADYTSRLSLMLTGGRHVCPVALLYLGQS
                     AQCGKHILPDQISESLQDALYDCDWLPFEVF"
     gene            1830..2759
                     /locus_tag="VIL39_00775"
     CDS             1830..2759
                     /locus_tag="VIL39_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY5479989.1"
                     /translation="MARDARIYVAGHRGLVGSAIWRELQRRGFTALIGRTRAELDLLD
                     AVGVREFYAQAKPEYVIDAAGRVGGILANSRHPAEFLFQNLQVQNNVIHGAYQAGVRK
                     LLFLGSSCIYPRLAPQPLKEEYLLSGPLEPTNEWYAVAKIAGIKMCQAYRRQYGCDFI
                     SAMPTNMYGPNDNYDLQGSHVLPALIRKFHEAKVANAPTVTCWGTGSPLREFLYSDDL
                     ARACVFLLENYSEEQFINVGSGSEVSVRELTETIKRVIGFSGQIVWDKTKPDGTPRKL
                     MDSSRLFALGWRPQVELESGIRLAYQDFLKQSG"
     gene            complement(2883..3101)
                     /gene="infA"
                     /locus_tag="VIL39_00780"
     CDS             complement(2883..3101)
                     /gene="infA"
                     /locus_tag="VIL39_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5479990.1"
                     /translation="MAKEDPIELAGMVTQVLPGTMFRVALPNGHEVLAHISGKMRKNF
                     IRISVGDQVNVEMSPYDLGKARITVRQS"
     gene            3315..3584
                     /locus_tag="VIL39_00785"
     CDS             3315..3584
                     /locus_tag="VIL39_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479991.1"
                     /translation="MTTVLLGVLTVSALLSVLFCWLYISNTRELRGLQVQAAMINNKR
                     AVINALANDVAEYSKKNPAIDPILESVGLKPGKSAPTATSKPTTK"
     gene            3591..3977
                     /locus_tag="VIL39_00790"
     CDS             3591..3977
                     /locus_tag="VIL39_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479992.1"
                     /translation="MDNLDNKIPLQAGDNDLQAQCDALRHLITSILILVIVISGTLNI
                     YLLRQWKTTSKDLAGIRPQATQMINEYGQKSGPLMDVFVNRIAEYGRTHPDFAPILDK
                     YNIKPVAATGAPAAAHTPTPAAKPKK"
BASE COUNT          873 a         1275 c         1204 g          845 t
ORIGIN      
        1 aaacacctcg aacggcagcc aatcgcagtc gtacagggca tcctgcaagc tctcgctgat
       61 ttggtcaggc aggatgtgtt tgccgcattg agcgctctgg cccagataga gcagcgccac
      121 tgggcacaca tgccgccctc cggtgagcat gaggctaaga cggctggtgt agtcggcaaa
      181 gacgcgatac agcggccagc gcggctcgta tccgccatcg tagaaatagg gtgggcaatc
      241 ggtgtcgtgg ggcgaccggg gattgaacga gtgcgggata agaaagttca cacccgaaac
      301 ctgcatgtgg tccgcccacc atttcatttc cggataggtg aggttctggc cgcgcgcgcc
      361 aaagatttcg accatggcca catcgtccgg cttgccgtag gcatggctga tggaactcgc
      421 cagcttgggg gtctgccagc aaggggcgtc gcgcgtgacg cgcttgccca tgacgaactg
      481 ttcgaacacc gcgtcaatcc cgcccatgtc gccgtggccc tgcaatcgca tcatgtcgcc
      541 cgcgcagaac tcccggttct ggtagaggtt ggcatgctcc atgaaatgtc cgatggactt
      601 caccccgtgc tggtggcacc actcggccat gccgccgtac atggtgcggc cccaggcttc
      661 tgcgaaggca tccagatact ggtagcgggc cgcgacctga tcctcgccgg ccagttcgga
      721 cttgtaagca acataggcct tcttccagtc cacgttccac tcagcgagca cggggttgag
      781 ttcggtgccc caatcaccgg gcgtctcggg ttcgtcatag aagaagccac gtatcgtctt
      841 gccgaaatcg gccctgtaat gctcaaagtg cggctggtaa accttcttga ggaaccagtc
      901 cacgcaatcc ctgctcgcgc cgtccacagt cagttgcgag cggccgacct gggcaagtcc
      961 gggcgcctgt ttgtgggtga acttgagaat gcgccatttc ccggccggag cctgccagcg
     1021 aagcttgccc ccgcgaatgt tcgggcccag atcaaccagg gtgttgccgt ccatcttgcc
     1081 ctcggcattc acctgcccgg caactgcggc cacatactta tcgccttcgt agccatcggc
     1141 ttcaaatgcg cgcggccctt ccagctcaac cgccgcagct tcgaggcgtt tggccgcgta
     1201 gcgcgggggc accttccccg ccaccgcctg gctgggccac catttctcgt caaagatcca
     1261 tagttgcaga ttctgcttct tcgccgccgc caggcagata tcgaggtcgc gccaccaacc
     1321 tgggcccatc caatcggtgt gcggacggga ttcggtggta aaggcgccgt tgccgccctc
     1381 ggccaccttg cccacgtaca tttcgagacg ttctttgctg tcgtcgccat gcaaccagaa
     1441 cagtggaccg gtgaggcggc gggcttcgag cggcagctcg cgaaattgtt gttcaacggc
     1501 agcaaagccc aaggcggagg agtgggcttc ggactcagcg gccagggcgg gcgtcatcgc
     1561 actgaacaaa accatggttg tgacgcaaag gagcgcagtg agtgcagtcg agggcatttt
     1621 cattatatgt gtaaaggcgc tccatgactc cacacaaagc tcctgagatc aatgccaatc
     1681 ttcaggaatg agacgggcgg gttgcgccgg ctggcccagg aatcccaccc ccattacttg
     1741 cagactcgtc atcgcaacgc tgaacggcga gttggcatgg tcaacgcttg tttcctgcgc
     1801 cgcattagtt tatgcctttg agcatggaca ttgcccgaga tgctcggatt tatgtggcgg
     1861 ggcaccgcgg cctggtcggc agcgcgatct ggcgtgaact gcagcggcgc ggattcaccg
     1921 ccctgattgg ccggacccgc gccgaactgg acctgctgga cgcagtcggc gttcgggaat
     1981 tttacgcgca ggcgaagccc gaatacgtta ttgacgcagc cggcagagtg ggtggcatcc
     2041 tggccaacag ccggcacccc gcagagttcc tttttcagaa tctgcaagtc cagaacaatg
     2101 tgatccacgg cgcttaccag gccggggtgc gcaagctgct gttcctgggc agttcctgca
     2161 tttatccgcg gctggctccg caaccgctca aagaggaata cctgctcagt gggccgctgg
     2221 aacccaccaa cgaatggtac gccgtggcca agatcgccgg catcaaaatg tgccaggcct
     2281 atcgccgcca gtacggttgc gacttcatca gcgccatgcc gactaacatg tacggaccga
     2341 atgacaatta cgacctgcaa ggctcgcacg tcttgcccgc gctgatccgc aagttccatg
     2401 aggccaaggt cgcgaacgcg cccaccgtca cctgctgggg caccggttcg cctttgcgcg
     2461 agttccttta ttcggatgac ctcgcccggg cttgtgtgtt tctgctcgaa aactacagcg
     2521 aggaacaatt catcaacgtc ggttccggca gcgaggtgtc cgtgcgcgaa ttgactgaga
     2581 ccatcaagcg ggtcatcggt ttctccgggc agatcgtctg ggacaagacc aaaccggacg
     2641 gcacaccgcg caagttgatg gacagctcac gcctgtttgc gctgggctgg cgaccgcaag
     2701 ttgagctgga atcgggtatt cgcctcgcct accaggactt cctgaagcaa tccggctgaa
     2761 agcctcccgc ctttccagcg gggcatatat gatgctggcc aggtcgcgcg tggcgaaggt
     2821 gcagtgatag attgacccgg tcgtcgtgcg agtagcgcca aggaactgaa ttcgcatcgc
     2881 ctctagcttt gccggacggt aatcctcgcc ttgcccaggt cgtaaggcga catttcaacg
     2941 ttcacctggt ccccgacgct aatgcgaatg aagttcttgc gcatcttgcc ggaaatatgg
     3001 gccagcactt catggccgtt cggcaaagcc acgcggaaca tcgtgcccgg cagcacctgc
     3061 gtcaccatgc cggccaactc aattgggtct tccttcgcca cggagtcagt tatcccgatc
     3121 tctagttgtt tcgtgctatt cgcttcggct gaaatcaatc acttttcagc gctccggcct
     3181 tagtcggcct tccgagagcc cggagattgc agttgtcggc ccccaagatc aagcaagttt
     3241 taaggtgaaa aacgtagtgg acatttgtga cggaatcgct acagtaccgc cccattgata
     3301 tgaaaaataa ccctatgact acggttctgc tcggggtgct gaccgtaagc gccctgctct
     3361 cggtgctgtt ttgttggctt tacatttcca atactcgcga actgcgcggg cttcaggttc
     3421 aggcggccat gatcaataac aagcgcgcgg tgatcaacgc cttggccaac gatgtggcag
     3481 agtacagcaa gaagaatccg gccattgacc caattctcga gtccgttggc ttgaaaccag
     3541 gcaagtccgc ccccacggcc accagcaaac ccacaactaa gtaggcgctt atggacaacc
     3601 ttgacaataa gatacccctt caagccggtg ataacgactt gcaagcgcag tgcgatgcct
     3661 tgcgtcatct gatcacgtca atactgattc tcgtcatcgt gatcagcggc acgctcaaca
     3721 tttacttgct gcggcagtgg aagaccacga gcaaggatct agccggcatt cggccgcaag
     3781 ccacgcaaat gatcaacgag tatgggcaga agagtggccc gttgatggac gttttcgtca
     3841 acagaatcgc cgaatacggg cggacccatc cggactttgc tccgatcttg gacaagtaca
     3901 acatcaagcc cgtcgcagcc accggcgcgc ccgcagcggc ccacacccca acgcccgccg
     3961 ctaagccgaa gaagtaaata gatcgtaaga gacccctgtt aggcccatga ccttctcggc
     4021 gctggggctc agccttaacc gcagaagaac cagaatctag agtgcggcgg acacatcgcc
     4081 ctgcatttgg agcagcttgg atgcgccgca gcatgacgcc gatcggtgat caggttgcgg
     4141 atgcaaggaa ctgagaaaac ggacacaacg tatccatacc tgccgggtta accggat
//
LOCUS       DATCGS010000042         9102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_647603, whole genome shotgun sequence.
ACCESSION   DATCGS010000042 DATCGS010000000
VERSION     DATCGS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647603"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..834)
                     /locus_tag="VIL39_00795"
     CDS             complement(10..834)
                     /locus_tag="VIL39_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479993.1"
                     /translation="MKTQDSMQAEDPSQTPSPLFPAPRITKLLLDLKASLGQAEGQSL
                     SYDDWAQIAGRPANTIASWCAGGAAHQLEVLLASMERLTAEERHRLIDRACRDYPTLR
                     HPKLAHDFVACSHLATLVRQPSGLTFIQGAPEHMRTFLLSALGNSASSPDLKKAAVAG
                     VDIHQPDQFVPVIGLIYLGNLLRSGEIERQFKQLWPTVRATRARLVLLNDIWRRVPAL
                     QPEIMDLARTSHVVVADALALKPGDLTSRVPTPTHLVAVSPAREQPAWIRVEIQAI"
     gene            complement(1236..1601)
                     /locus_tag="VIL39_00800"
     CDS             complement(1236..1601)
                     /locus_tag="VIL39_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479994.1"
                     /translation="MKRRTLQRIPPGRQSEHHHNVYVVLLDPAAGKILSVREANPNRN
                     PKKPCVYVGMTGLKPEERIANHKRGIKSASVVKRYCIRLLPELYAHLNPMPFEAAAQM
                     EQDLAEDLRRAGYTVTGGH"
     gene            complement(1626..2141)
                     /locus_tag="VIL39_00805"
     CDS             complement(1626..2141)
                     /locus_tag="VIL39_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479995.1"
                     /translation="MTEQQFRAVYPLISGWIRQTLAEHASAARPVASLGFKRLPHYYD
                     EPMLTSTKVVFVPKVPVPPLSALGLDRFSDFERMDAAGITYLDTYFVRADPDPDESLH
                     FHELVHVIQWRLLGPERLLALYADGLERFGYRDSPLEVMAYTLQDRFERGTQPFGVEA
                     ACQSLIREMAK"
     gene            complement(2167..2670)
                     /locus_tag="VIL39_00810"
     CDS             complement(2167..2670)
                     /locus_tag="VIL39_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479996.1"
                     /translation="MGHDLILYQFSRSKVERGDFSHFLSLYAADKLPEGRRLREMMGR
                     IVFCIEGWDSDPREIHMVPEIRRFYSAFHGVWPYWLYFCNLDADTLRAIAMCCLPEVN
                     TMQVYGYTKVAVTCDPMDILNFLKRDFLPMNLMCERAEMFERGIYDRTKAVFEYFSLP
                     FDAEPPP"
     gene            complement(2698..3087)
                     /locus_tag="VIL39_00815"
     CDS             complement(2698..3087)
                     /locus_tag="VIL39_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5479997.1"
                     /translation="MLQRLWRIAAVSAKPFLRRSAAIGNRAHGDPSFSSVAICSGVWY
                     SEPMLQKKLGQRVAELRKARQLTQPELAKRIACSVEFISLVERGVNAPSVAGLEVFAK
                     ALKVEVVDLFTFSQRTRPRKPHPAKQT"
     gene            3086..3454
                     /locus_tag="VIL39_00820"
     CDS             3086..3454
                     /locus_tag="VIL39_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5479998.1"
                     /translation="MQKMSKVILGLAERVLAQPPEKTSSESAAAALLAHIAWNRAVDP
                     LGGDQIGYYRKVLRALERENPKCRRELKSMDCEALIQELMNLKLALYPADDRIIRLCG
                     ITERKTVRVEWHYRGVEGVN"
     gene            3510..4595
                     /locus_tag="VIL39_00825"
     CDS             3510..4595
                     /locus_tag="VIL39_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY5479999.1"
                     /translation="MKQPDPSMVPIRAAVLRTPGRPLKIERLEMEGPRDDEVLVRLVA
                     SGICHTDIDFCDSGGSDPVVLGHEGSGVVERIGRRVEGIKPGDHVVLSYQSCGRCQPC
                     RNGHPADCQRFWEANFGFARLDGTNALRGGVRGHFFGQSSFATHALATVRNLVKVPKP
                     LPLALLAPLGCGLQTGAGTVMNSLAVRSGSSIAVFGTGSVGLAAVMAARILKAKTIIA
                     VDINHHRLKLARELGATHSINNRRSDIAGSIKAITGSGVDYVVESTADHDMEQLAAEL
                     LNPGGKAALLSGAGAPGKLAGGRKVLSVIQGDAVPQKFIPKLIKLHQDGRFPFDRLVK
                     FYDFAKINQAMADSKRGSTVKPVLLIS"
     gene            4764..5345
                     /locus_tag="VIL39_00830"
     CDS             4764..5345
                     /locus_tag="VIL39_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY5480000.1"
                     /translation="MKNSIIDRSKTALVVIDLQKGIVGRQTAPHASDLVVKNAAALAE
                     AFRKHSMPVFLVRVAPSPDGKDALRPIVDAPAQAQTPPPDWADLVPEVGPKPGDFVIT
                     KHQWGAFYGTELDLEFRRRGIATMVLCGISTNIGVESTARFAYEYGYNQIFAEDAMSA
                     MSIEEHTTTVTKIFPRIGLVRKTKEILADLLHD"
     gene            5687..6916
                     /locus_tag="VIL39_00835"
     CDS             5687..6916
                     /locus_tag="VIL39_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480001.1"
                     /translation="MKLQNERLVLLLLAAIQFATNLDFLIILPLGPQYMRVLHLTPAQ
                     FNLIVAAYAIAAGISGIAAGFFLDRFDRKTALLWLFLGFATGTLLCALAPTYHLLVAA
                     RAIAGTFGGVTGAVILAIVGDVIPEYRRCAAMGLVMSAFSVASIFGVPLGLVLASCFS
                     WHVPFYVIAGISVPILLVVFRFVPALRGHLHHPRDQHPAARMLAVLTEPSHQIAFLFM
                     AVLTCAGFTIFPNLATYMVYNVGLTEKQLPFIYLTGGICTLFSMNWVGRWADRAGKLR
                     VFTLMSLAASIPVIALTNLARVPLLVALAISTLLMICMSGRFVPAMAMMTATVEARYR
                     GGFMSVNSSVQQFSCGLAAWISGGIIGQGPNQEITHYPVAGVVSLACMLICIWLARFL
                     RAAPGELTSAPVFVESV"
     gene            6826..8220
                     /locus_tag="VIL39_00840"
     CDS             6826..8220
                     /locus_tag="VIL39_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480002.1"
                     /translation="MYAHLHLAGAVPPGRPRGIDLGPGLRRIGLTHPGHPPSWICRGR
                     FGAFFVNLSPSFLRTRSATDFAAILVEGEELNAFLLAPDRRSFLHPELQAELQFGIEQ
                     SKDQTIASVMENLTAFLEQTKEWQKADNEIVGLRGGLTRRALPGTDDLEAAVGHEIDY
                     EQSVWQGDYAAAFEACREVLARLNDDSLRGYRALWSYLAGSAAWLGFKGGIPGFDQQA
                     RTQYAAAQKAARRVAWLVGLCRVQEAPDAAPTRDEALALQVEKLEGVLEELGTTHDRK
                     FNEELLFIGDNIMEADSKKFEPAHERLGTLLGFAAGKIESTGSPDPWWILSDGVCLVF
                     EDYSNAEPSSCLGVTKARQVASHPNWMRENVPLSKSAHVIPVLVTPVSLVDKDAMPHL
                     KEVCIWKIDEFRALAKTALLVIRELRRTFPGSGDLAWRAAAAERLQAAQLDPASITGL
                     VISRSADRVVQVKT"
     gene            8264..>9102
                     /locus_tag="VIL39_00845"
     CDS             8264..>9102
                     /locus_tag="VIL39_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480003.1"
                     /translation="MRLLRQIIATALLLAAIAAPGQANTIILATLNCYWFFDAQEGKA
                     SADKPTSTLEYSTKAGHLIGLLPREAPLFVGFQEIGGGEDLAALAHSASARYGHTYQI
                     LFARGKDISTGQNVGAILDMSSGWGIYGRPSRVSDLERELSKHLVVRLTNAVTSMDIC
                     VVHLRRPIGSDGIEKQKNQCRALLKWAMRHLSGDPKANLVILGDFNEGHPVGSDSQAL
                     DVLFRAKPPLVDALGTLSGKVTTHTDGKAYDRSLVSDAIAKGLNRLKLERVVIQPHRH
                     GKG"
BASE COUNT         1753 a         2708 c         2804 g         1837 t
ORIGIN      
        1 gaatctggtt cagatcgcct ggatttcgac ccgaatccac gccggctgct cgcgagctgg
       61 cgaaaccgca accaggtgag ttggggtcgg aactcggctg gtcagatcgc cgggcttgag
      121 ggcgagtgca tcggcgacaa cgacgtgcga ggttcgggcc aaatccatga tttccggttg
      181 cagcgccggc actcgtcgcc agatgtcgtt gagcaggacc agccgggctc tggttgcgcg
      241 cacagtcggc caaagctgct tgaattgccg ctcgatctct ccagagcgga gcaggttgcc
      301 caggtagatc agcccgatga cgggcacaaa ctggtccggt tggtggatat ccacccccgc
      361 cacggcagcc ttcttgagat ccggggaact ggccgagttt cccagggcgc tcagcaggaa
      421 ggtgcgcatg tgctcgggcg caccctggat aaacgtgagc ccggaaggct gccgcaccag
      481 cgtggcgagg tggctgcagg ccacaaaatc gtgcgccagc ttgggatggc gaagcgtggg
      541 ataatcccga caagcacggt cgatcaatcg atggcgttcc tccgcggtca aacgctccat
      601 ggaggcgagc agcacctcga gctggtgggc cgctccgccc gcgcaccaac tcgcgatcgt
      661 gttcgccgga cgtccagcga tttgcgccca gtcgtcgtaa ctcaacgatt gcccctccgc
      721 ttgaccgagg ctggccttga ggtccaacag cagcttcgtg atccgcggcg ccgggaacag
      781 gggtgagggg gtttggctgg ggtcttccgc ttgcatcgaa tcttgtgttt tcatgtcttc
      841 gtgttggccg agattttgga gcgccaatga aatccaggtc ggacgttcta agactcgctt
      901 gaggattatc ctccgcgaca ggattcgcga gagggtctct gacgttgggc cagggcggtt
      961 cccgtcgcgg ctgtccgggc taggaagcac ccatgcatgg ttgcgccagc cggacgcttc
     1021 aacccttttg caaaatcatt gaccacccgc tttctctcgg cctcgtcttt gcttggagcg
     1081 acagagggtc tgaatgttgt tagcttcttt tcgtcatgga ttggccgatg gtgggatcag
     1141 cagttacccg gccacagcct cccggcgatg gcggatcacg gagttcgaaa acccttccaa
     1201 aaatcgtgcg cggaaggggt tcgacaccct gcaggctaat gtccccccgt gaccgtgtag
     1261 ccggcccggc gcaaatcctc tgccaaatcc tgctccatct gtgccgctgc ctcaaagggc
     1321 attgggttga ggtgcgcgta gagttccggc aacagacgga tgcaataccg ctttacgacg
     1381 gatgccgact tgattcctcg tttgtggttg gcaatgcgtt cctcgggctt tagcccggtc
     1441 atccccacat acacacatgg cttcttgggg ttgcgattgg ggttggcctc gcgaacgcta
     1501 aggattttgc cagcagccgg gtcgagcaac acgacgtaga cattgtgatg gtgctctgat
     1561 tgcctgccgg gcggaatcct ctgcagtgtt cgacgtttca ttcggcatcc accggaaggt
     1621 tgccctcact tggccatctc ccgaatcaag gattggcagg cggcctcgac cccgaagggc
     1681 tgtgtcccac gctcaaacct gtcttggagg gtgtaggcca tcacctccag cgggctatct
     1741 cgatagccga accgttccaa accatcggcg tagagcgcca aaagcctttc cgggccaagg
     1801 agccgccact ggatgacgtg aaccagttcg tggaaatgga gggactcgtc ggggtcgggg
     1861 tcagcacgga cgaaataggt gtcgaggtag gtaatgcctg cggcgtccat tcgctcgaag
     1921 tcgctgaacc ggtcgaggcc cagcgccgac agcggcggga ccggcacctt gggcacaaac
     1981 acgacttttg tggaagtcag catcggctcg tcgtagtaat gcgggagacg cttgaaaccg
     2041 agcgatgcca cgggccgtgc tgccgatgcg tgctcggcta aggtctgccg aatccagcct
     2101 gagatcaggg gataaacggc ccggaattgc tgctcggtca ttggggcaag tgtcgtttgg
     2161 tagtggtcag ggcggcggtt cggcatcgaa cggcaggctg aagtactcga acaccgcctt
     2221 cgttcggtcg taaatgccgc gctcgaacat ctccgcccgc tcgcacatca ggttcatggg
     2281 cagaaaatcc ctttttagga agttcaggat gtccatgggg tcgcaggtga ccgctacctt
     2341 ggtgtagccg tacacttgca tcgtattcac ttccggcagg cagcacatgg caattgcccg
     2401 aagcgtgtct gcgtcgaggt tgcaaaaata gagccagtag ggccaaactc cgtggaaagc
     2461 cgagtaaaac cgccggattt ccggcaccat atgaatttct cgcgggtcgc tgtcccagcc
     2521 ttcgatgcag aatacgatgc ggcccatcat ttcgcgaagc cgacggccct caggcagttt
     2581 atcggccgcg tacaggctga ggaaatggct gaagtcgccg cgctccacct tcgagcggct
     2641 gaactggtaa aggataaggt cgtgtcccat gctggcctcg gttcgtcttc gcggcggcta
     2701 ggtctgcttc gccgggtgtg gtttgcgcgg acgggtcctt tggctgaagg tgaacaggtc
     2761 aactacctcc accttcagcg ccttagcgaa aacttccagc ccggcgacgg acggggcatt
     2821 gaccccgcgc tcgaccaggc tgatgaactc caccgagcag gcgatccttt ttgccagctc
     2881 tggctgcgtc agctgccgcg ccttgcgcaa ttccgcgacc cgctggccaa gtttcttctg
     2941 gagcatgggt tcggaatacc agacgccgga gcagatggca acggagctaa acgacgggtc
     3001 gccgtgagcg cgattgccga tggctgcgct ccgccgcaaa aatggtttcg ccgaaacggc
     3061 ggcgattcgc cataatcgtt gcagcatgca gaaaatgtcc aaggtcattc ttggcctggc
     3121 cgagcgggtg ctggcccaac cgccggaaaa gacgagcagc gagagcgccg ccgccgccct
     3181 tctggcgcac attgcctgga atagagccgt ggatcccctc ggtggggacc aaatcggcta
     3241 ctaccgcaag gttttgcgcg ctttggaacg ggagaatccg aagtgccggc gggagctgaa
     3301 aagcatggac tgcgaagccc tgattcaaga gctcatgaac ctcaaactgg cgctctatcc
     3361 cgccgatgat cggattatcc gtttgtgcgg catcacggag cgcaagacgg tgcgcgtgga
     3421 atggcactac cgaggagtag aaggcgtgaa ctgaccctcc cgccttcatt taccttcact
     3481 gccgcagtga attggcatgg agccaacgca tgaaacagcc agacccgtcc atggtcccca
     3541 tccgagccgc ggttctgcgg acgcccggac gcccgctgaa gatcgagcgc ctggaaatgg
     3601 aaggtccacg cgacgacgag gtgctcgtgc gcctggtggc cagcgggatt tgccataccg
     3661 acattgattt ctgcgacagc ggcggttccg acccggtcgt gctggggcac gaaggctccg
     3721 gcgtggtgga acggatcggc aggagggtgg agggaatcaa gcccggggat cacgtggtgc
     3781 tctcctacca atcctgcggg cgctgccagc cttgcaggaa cggccacccg gccgactgcc
     3841 agcggttctg ggaggcgaac tttggcttcg cccggttgga cgggaccaac gcgctgcgcg
     3901 gcggcgtgcg gggtcacttc tttggccaat catcgtttgc cacgcacgcg ctggcgaccg
     3961 tgcgcaacct ggtgaaggtg cccaagccgc tcccgctggc attgctagcg ccgctgggct
     4021 gcggcctgca gaccggcgcc ggcaccgtga tgaactcgct agcggttcgc tccggatcca
     4081 gcatcgccgt ttttggcact ggctcggtgg gtctggccgc ggtgatggcc gcgcgcatcc
     4141 tcaaagcaaa aacgatcatc gcggtggaca ttaaccatca tcggctcaaa ctcgcccgcg
     4201 aattaggtgc gacccactcc atcaataacc ggcgaagcga catcgccggg agcatcaagg
     4261 cgataacggg cagcggcgtt gattatgtcg tggagagcac agcggatcac gacatggagc
     4321 agctcgccgc tgaactgctg aatccaggcg gaaaagcggc gctgctcagc ggagcgggcg
     4381 cgccgggcaa actggcggga ggccgcaaag tcctcagtgt catccaaggc gacgctgtgc
     4441 cccagaagtt cattccgaag ttaatcaagc tgcaccagga tgggcggttc cccttcgacc
     4501 gcttggtgaa gttctatgat ttcgccaaga tcaaccaggc catggccgac tcgaagcgcg
     4561 gcagcaccgt caagcccgtg ctgctgatca gctgatcaga tgctcgattc agcggaacag
     4621 gctgctgacg cggtttttgc caccaggctc gccaaagatc taactcagcc tggcggaacg
     4681 cgcggctgct ggagtgcgtg agcggtttga atccagaaaa gcagtgctta acctcaaaag
     4741 aaaaagggaa tacgctgatg gctatgaaga attcaatcat tgatcgatca aagacggcgc
     4801 tggtggtcat cgatctccag aagggcatag taggacggca aaccgctccc catgcgtcgg
     4861 acctcgtcgt gaagaacgcg gcggctcttg cagaagcgtt caggaaacat agcatgccgg
     4921 tgtttttggt cagggttgct ccatctccgg acggaaagga cgcactgcgc cccatcgtcg
     4981 acgcgccagc gcaggctcaa accccgccgc cggattgggc ggaccttgtt ccggaagtgg
     5041 gaccgaaacc gggcgatttc gtgataacca aacaccaatg gggcgctttc tacggaacgg
     5101 aacttgattt ggaattcagg aggcgcggca tagcgaccat ggttctgtgc ggcatctcga
     5161 caaatatcgg cgtagagagc actgcccggt tcgcttatga atacgggtac aatcagattt
     5221 ttgccgaaga tgccatgtcg gccatgtcga tcgaggagca cacgaccaca gttactaaga
     5281 tatttccccg tattggcttg gtgagaaaga ccaaagaaat actggccgat ttactgcacg
     5341 attaagcttc accgattcgg gctgacaatt cgattgaact gggggagcaa attaagagct
     5401 cacgtggcgg cccggacatc aaaacctgct ggagagctcg ccttctgacg tgggcaggcg
     5461 ggccgggacg tggcgacggc acatcggcgt cggagatgcg gtctggcgaa aagctgtcgc
     5521 catcaaaacg gtgaattggg aacccgcccg agttcccttt tccactcgca ccgtggcgat
     5581 tccatgctgc gtttcgggcc ttggacattg cgtcccaaaa cgcttccgcg cgaaacaact
     5641 caatacgagg cgttacggcc gagcagtgaa cctggaattt tgagctttga aactacagaa
     5701 cgagcgattg gtcctgctgc tcctggcagc catccagttt gccaccaacc tcgatttttt
     5761 gatcatcctg ccgctgggcc cccaatacat gcgcgtcctg cacctcaccc cggcccagtt
     5821 caatctgatc gtcgcggcct acgccatcgc ggcgggaatc tccgggatcg ccgccgggtt
     5881 tttcctcgat cgctttgatc gcaagaccgc cctcctctgg ctctttctgg ggttcgccac
     5941 cggcaccctc ttgtgcgcgc tggcccccac ctaccacctt ctggtggctg cccgggccat
     6001 tgccggcacc ttcggcggcg tgacgggcgc ggtgattctc gccatcgtcg gcgatgtcat
     6061 cccggaatac cggcgttgcg cggcgatggg cctggtgatg tccgccttct ccgtcgcctc
     6121 gattttcggg gtgccgctgg ggcttgtgct cgcttcgtgt tttagctggc atgtgccgtt
     6181 ttacgtcatt gccgggatca gcgtgccgat tctcctggtc gttttccggt tcgtgcccgc
     6241 cctgcgcggc catttgcacc atccgcgaga ccagcatccg gcagcgcgga tgctggccgt
     6301 gctcacggag ccgagccatc aaattgcctt tctgttcatg gcggtgctca cctgcgccgg
     6361 cttcaccatc tttcccaacc tggccaccta catggtgtac aacgtgggac tgaccgagaa
     6421 gcaactgccg ttcatctatt tgaccggcgg aatctgcacg ctcttcagca tgaactgggt
     6481 gggacgctgg gccgaccgcg ctggaaagct ccgcgtcttc acgctgatgt cccttgctgc
     6541 gtcgatcccc gttatcgccc tcacgaatct cgcccgggtt cccctgctcg tggcgctggc
     6601 gatctcgacg ctgctcatga tctgcatgtc cggccggttt gttccggcca tggcgatgat
     6661 gacggcgacg gtcgaagcgc gctatcgcgg cggcttcatg agcgtcaatt cctccgtcca
     6721 acaattctcc tgcggcctgg ctgcatggat cagcggaggc atcatcggcc agggacccaa
     6781 tcaggaaatc acccactacc cggttgcggg cgtggtgtcc ttggcgtgta tgctcatctg
     6841 catctggctg gcgcggttcc tccgggccgc ccccggggaa ttgacctcgg ccccggtctt
     6901 cgtcgaatcg gtctgacaca tcccggacat cccccttcat ggatttgccg cggccgattc
     6961 ggtgccttct tcgttaacct ctccccaagc ttcctacgca cccgctccgc cactgatttc
     7021 gccgcaatcc tagtggaagg agaggaactt aacgcctttc tgcttgctcc cgatcgacgc
     7081 agcttcttgc atccagaatt gcaggcggag cttcagtttg gcatcgagca atcaaaggat
     7141 cagaccatcg cgagcgtgat ggaaaacttg accgcgttcc ttgagcagac gaaggagtgg
     7201 caaaaggcgg acaatgaaat tgtcgggctg cgagggggcc taacccgcag agcgttgcct
     7261 ggcacggatg acctggaagc cgccgtgggc cacgagattg attacgagca aagtgtttgg
     7321 cagggggact acgctgctgc ctttgaagct tgcagggagg ttcttgctcg cctgaacgac
     7381 gattcgctcc ggggttaccg tgccttgtgg agttacttgg cgggtagcgc agcttggctt
     7441 ggatttaagg gaggcattcc gggctttgac caacaggctc gaacccaata tgcagccgcg
     7501 cagaaagctg ctcgtagagt ggcgtggctg gttggcctgt gccgggttca agaggctccc
     7561 gacgctgctc cgacccgcga cgaagcactt gcgctccagg tcgagaagtt ggaaggggtt
     7621 ctcgaagaac tggggactac ccacgaccgg aagttcaatg aggagctgtt gttcattggt
     7681 gacaacatca tggaagctga cagcaagaaa ttcgaaccgg ctcatgagcg cctcggcacg
     7741 ttgcttggct tcgcggctgg aaagattgaa tcaaccggct caccagaccc ctggtggatt
     7801 cttagcgatg gggtttgtct ggtgtttgag gactattcaa atgccgagcc gtccagctgc
     7861 cttggcgtta cgaaggctcg gcaagtcgct tcacatccca actggatgcg ggaaaacgtc
     7921 cccctgtcca agagcgctca cgtgatccct gtgctagtca cgccggttag cctggttgat
     7981 aaagacgcca tgccgcactt gaaggaggtc tgcatctgga agattgacga atttcgggca
     8041 ttggcgaaga cggcacttct ggtgattcgc gaacttcgca gaacctttcc cggctccggc
     8101 gatttggcgt ggcgggctgc cgctgccgag cgtttacaag ccgcgcaact cgatccggct
     8161 tcgataaccg gccttgtcat ctcgcgatcc gcagaccgag tcgtccaggt gaagacgtga
     8221 ttgacgcaat gatttgtttg ggaaaaccca tctgctttca ctcgtgaggc tcctccgcca
     8281 aatcatcgcg accgcgttgc tgctggcggc gatcgctgcc ccaggtcagg cgaatacgat
     8341 tatcctggcc accctgaact gctactggtt cttcgacgcc caggagggca aggcgagcgc
     8401 cgacaagcca acgtccaccc tggaatacag caccaaggcc gggcacctga ttgggctgct
     8461 acccagggag gccccgctgt tcgttggctt ccaggagatc ggcggcggcg aagacctggc
     8521 ggcgctggcc cactccgctt ctgcgcgata tggccacacg tatcagatcc tgtttgctcg
     8581 cggcaaagat atatccaccg gccagaacgt cggcgcgatc ctcgacatgt ccagtgggtg
     8641 gggcatttac ggcaggccgt cgcgagtgtc cgacctggag cgcgagctgt ctaagcacct
     8701 ggtcgtgcgg ctcacgaacg cggtaacctc gatggacatc tgcgtcgtcc acctgcgcag
     8761 gccgatcggc agcgacggca ttgagaagca gaagaaccaa tgccgggcgc tgctcaagtg
     8821 ggcaatgcgg cacctgtccg gcgacccgaa ggccaacctt gtcatcctgg gcgacttcaa
     8881 cgagggtcat ccggtcggca gcgacagcca ggcgctggac gtgctgttcc gggcaaagcc
     8941 gcccctggtg gacgcgctcg gcacattgag cgggaaggtt acgacccaca cggacggcaa
     9001 agcctacgac cgcagcctgg tgtctgacgc gatcgccaag ggcctgaacc ggctcaagct
     9061 tgagcgggtg gtcatccagc cgcaccggca cggcaagggg ga
//
LOCUS       DATCGS010000043         5619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_301190, whole genome shotgun sequence.
ACCESSION   DATCGS010000043 DATCGS010000000
VERSION     DATCGS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301190"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VIL39_00850"
     CDS             <1..671
                     /locus_tag="VIL39_00850"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY5480004.1"
                     /translation="ADALPLRGAAGTSGNGPATGSGPLRTLGAALHYLTLSWYPFRRG
                     ICPHCGERRSIRSPLLEVATIAAFGLAWWRFAASPLHLVVVCLYAAFLLAVLVIDFEH
                     RRVLNVMLAPAAVVALLVSLLPGHPDLSQALLGGGLGGGIFLLLGLIGRGAIGAGDIK
                     LAGVIGLLTGYPLVIPALLLGVGLGGAAAFTLLVTRRAGRKSSMAYAPYLAVGAIIVL
                     MMGS"
     gene            761..1087
                     /locus_tag="VIL39_00855"
     CDS             761..1087
                     /locus_tag="VIL39_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5480005.1"
                     /translation="MTRGAPVVVSGRVASTFWSRFRGLMGVSSLAPGAGLVIEPCSSI
                     HTHFMRIPIDVLYVDQDHVIAGIDRNLRPWRIGRFYKKVHYVVELPAGGAAGCQVGDR
                     LQINGV"
     gene            1244..3487
                     /locus_tag="VIL39_00860"
     CDS             1244..3487
                     /locus_tag="VIL39_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5480006.1"
                     /translation="MANYQRMTKAELIPALRGLEAQVASLESIAAERDRLSHELSVHQ
                     IELEAQNQELREAQRLVEESRDRYADLYDFAPVGYVALDAGGHIQEINLTGASMLGVE
                     RSRLIGIPFSLYVARSDAGAFREHLRQIGQSQEPVTGELQVVVKEAQAIRVQMVSLAL
                     RDGGGGISRYRITMRDVTAHRRAEQALRESEERFRAIFEQAAVGVSQCVSHTGEYVRV
                     NRKYCEIAGYAREELLTWRFQGITHPDDLEAELNLMQQLMEGKIRTFSMEKRLRRKDG
                     EIVWVNLTVSSMWASGEEPTYHIAIIEDITERRQAIEDTKLRLAELEPVSEISTTLRA
                     ARTLGEMLPMLLDLTLEALHAMEGSIWLYDSVEDVLRPAASRGESEKDGTCPHPLKPG
                     EGIVGAAFATGQPHLAGDFRLDQGASEAMCQRIPPGIGGAAIPIRAGEKVIGTFVINV
                     SLPRKLTPSEVHLLTTLSELAGTAIQRIMLREEVERRAAELEKRVIQRTAELSQREAA
                     LEAANAKLTELDRMKSQFVSNVSHELRTPITTIKLYASLLRRGPPERRDQFLDALEQE
                     ANRQAHLIEEILQVSRIDSGRLDLHYAPYALNDLAEASVVSREPLAAEKGVTLACKPL
                     KPGPTVSVDRVRTLQVIDNLVENALWYTPTGGQVSVATGTAEADGQIWATLSVTDTGM
                     GIPKDELPRIFERFFRGGKPREMQLPGTGLGLAIVKDIVDLHGWRVTVESQVGGGSTF
                     TVWMPLA"
     gene            3523..4632
                     /locus_tag="VIL39_00865"
     CDS             3523..4632
                     /locus_tag="VIL39_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5480007.1"
                     /translation="MDEPRILIVEDHEPLRVAIQTILEAEGWSVLSAGDGLEGLEVMS
                     QVCPDLILADIMMPRMDGLAFYGAVRAGREWMAIPFIFLTAKTSREDALKGIELGAED
                     YITKPFEPEELVVAVRARLKRAEALRAVTEAEFDRLRQQIIAIPRQLLEAQEAERRSI
                     ARELHDEIGQLLTGLRLILEMAGRELPGARADSLTQAQALVGELLARVREMSLQLRPP
                     MLDDLGLLPALMWHFERSAALTQVRVNYKHMGVEDRRFPQEVETAAYRIAQEALTNVA
                     RHAQVIEVTVRLWAVQEMLGLEIEDRGVGFDAASVLATHSSSGISGMRERAFLLGGHF
                     TVESAPGKGTRILVELPFGGNTVREQHERAYSACG"
     gene            4604..5254
                     /locus_tag="VIL39_00870"
     CDS             4604..5254
                     /locus_tag="VIL39_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480008.1"
                     /translation="MSVRILLVDDHPIVRQGLRTLLQAEAGFAVVGEASNGLEALRLI
                     ERYKPDVLVVDLMMPGVTGLEVVHQTTQLSPNTRVIVLSMHSDEAYVLEALKSGAMGY
                     VLKEANPTDLIQGVHEVIAGRRYLSAPLSERAIEVYFQRGQEAGEDPYETLTTREREV
                     LQLAAQGLTNHGIAAALSISSRTVEIHRANMMHKLGLRHQADLVRYAARRGLLPLE"
     gene            complement(5291..>5619)
                     /locus_tag="VIL39_00875"
     CDS             complement(5291..>5619)
                     /locus_tag="VIL39_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5480009.1"
                     /translation="SLERLSAEKLAAVYPTHFGVVSDVREHLDTLDRLLDEAAGFVRA
                     RLQAGAAREDLIAEYTAWDRARANAWQVGEEEFQAYAATNPHFMSVDGLSRYWRKRLE
                     AETSSS"
BASE COUNT         1068 a         1617 c         1863 g         1071 t
ORIGIN      
        1 cggctgacgc attgcctttg cgcggcgcgg ccggcacatc gggcaacggc ccggcgacag
       61 gctcaggacc gttgcgcacg ctgggcgcag cgttgcatta tctgaccttg tcgtggtatc
      121 cgttccgtcg cggcatctgc ccacactgcg gcgagcggcg gtcgatccgt tcgccgctgc
      181 ttgaggtggc cacgatcgcc gccttcgggt tggcgtggtg gcgtttcgcg gccagcccac
      241 tacacctggt ggtcgtctgt ctctacgccg catttctgct ggcggtcctg gtgattgact
      301 tcgagcaccg ccgcgtgctc aatgtcatgc tggcgccggc cgcggttgtt gcgctgctcg
      361 tcagcctcct gcccggccac ccggatctgt cccaggcatt gctcggcgga gggctcggcg
      421 gcggcatctt cctgctgctg ggactgatcg gccgcggagc cataggcgcc ggtgacatca
      481 agctggcggg cgtcatcggc ctgctgaccg gctatccgct ggtcattccg gcgctgctgt
      541 tgggtgtcgg gttgggtggc gcggcggcct tcaccttgct cgtcacccgt cgcgccggcc
      601 gcaagagcag catggcctac gcgccctacc tggcggtcgg cgcgatcatt gtgttgatga
      661 tgggaagtta ggacgcctcc tactcttcgg cgatttgcca tttgggatgt gccatctgcc
      721 tatgccatac aatcctccct ccccaatacg tgttgaaaac gtgacccgcg gtgccccggt
      781 cgtcgtgtct ggacgcgtcg cgagcacctt ctggtcccgt tttcgcggac tgatgggcgt
      841 ctcatcgctg gcccccggcg cggggctggt cattgagccg tgcagttcca tccatactca
      901 cttcatgcgc atcccgattg atgtgctgta cgttgaccag gatcacgtga tcgccggcat
      961 tgaccggaac ctgcggccgt ggcgcatcgg ccggttctat aagaaggtgc actacgtagt
     1021 ggagctgcct gccggtggcg ccgcgggatg ccaggtgggg gatcgcctgc agatcaacgg
     1081 agtgtaatgg accgctagcg cgtcgagcgc aagcagttgg tgttgaatgt atgccacatg
     1141 taaggagagg ttgaccggac ggggttgatc tcgttggcga tggcagaagt aggggcgagc
     1201 gcgtggggag aggttgtagg cgcccgcgtc caagaggtga tggatggcaa actatcagcg
     1261 aatgaccaag gcagaactga tcccggcact ccgcggcctt gaggcccagg tggcgtcgtt
     1321 ggaatccatc gctgcggaac gcgatcgcct gtcgcacgag ctctcggtcc atcagatcga
     1381 gctcgaggcg cagaaccagg aactgcgcga agcgcagcgg ctggtggagg aatcccgcga
     1441 tcgttatgcc gacctgtatg actttgcccc tgtgggctat gtggcgctcg acgccggtgg
     1501 gcacatccag gaaattaacc tgactggcgc aagcatgctg ggtgtggagc ggtcgcggct
     1561 gattgggata ccgttcagcc tatatgtcgc caggagcgat gccggcgcgt tccgcgagca
     1621 cctgcgtcag atcgggcagt cccaggaacc tgtgaccggt gaactccagg tggtggtgaa
     1681 agaggcgcaa gcgatccggg tgcaaatggt gagcctggcg ctgcgagacg gtgggggagg
     1741 gatctcacgc taccgcatca ccatgaggga tgtcaccgcg cacagacggg ccgaacaggc
     1801 gctgcgcgaa agcgaagagc gcttccgcgc gatattcgaa caggctgcgg tgggtgtgtc
     1861 gcagtgtgtt tcgcacacag gggaatacgt gagggtcaac cggaagtact gcgaaatcgc
     1921 aggttacgct cgtgaagagc tgctgacctg gagattccag ggcattacac atccggatga
     1981 cctggaggcc gagctcaatc tcatgcagca gttgatggaa ggcaagatcc gcactttctc
     2041 aatggaaaaa cgcctccgtc gcaaagatgg tgaaatcgtc tgggtcaatc tgaccgtctc
     2101 ttccatgtgg gcaagcggcg aagagcccac gtaccacatt gcgatcattg aagacatcac
     2161 cgaacgcagg caggcgatag aagacaccaa gctccgcctg gccgaacttg aaccggtgag
     2221 cgaaatatct accacgctgc gcgccgcccg aaccttgggc gaaatgcttc cgatgttgct
     2281 ggatctaaca ctggaggcgc tgcacgcgat ggagggaagc atctggttgt acgacagtgt
     2341 cgaggatgtg ctgcgtccgg cggcctcacg tggggagagt gaaaaggatg gaacgtgtcc
     2401 ccacccgcta aagcccggtg aggggattgt cggcgccgct tttgcgaccg ggcagcctca
     2461 cttagcaggc gattttcgtc tggatcaggg cgcatcagaa gcgatgtgcc agcgaattcc
     2521 gcccggcatt ggtggagcag ccatcccaat tcgcgccggg gagaaggtaa tcggcacgtt
     2581 cgtgatcaac gtgtccttgc cccggaaact gacgccgagc gaggtccatc tcttgaccac
     2641 cctgagcgag ctcgccggca ccgccatcca gcgcatcatg ctccgcgaag aggtggagcg
     2701 ccgcgcggcc gagctggaga agcgggtgat ccagcgtacc gctgagttgt cccaaaggga
     2761 ggccgcgctc gaggcagcca acgcgaagct cacagagctc gatcgcatga agagtcaatt
     2821 cgtctccaac gtctcccatg agttgcgcac gccgatcacg acgatcaaat tgtatgcgtc
     2881 gttgttgcgg agagggccgc cggaacggcg tgatcaattt ctggacgcgt tggagcagga
     2941 agccaaccgg caagcccacc tgatcgagga gattctgcaa gtctcccgta tcgactccgg
     3001 gcggctcgat ctccactacg caccctacgc cctaaacgac ctggcggagg catcggtggt
     3061 cagccgggag ccactggctg cagaaaaagg ggtgacgctc gcctgcaagc cgctcaaacc
     3121 ggggccgacg gtgtcggtgg atcgcgtacg cacgctacaa gtgattgata acctggtcga
     3181 aaacgcactg tggtatacac cgacaggggg acaagtaagc gtcgccaccg gcacagccga
     3241 agccgatgga cagatctggg cgacgttgag cgtgaccgac accggcatgg gcatcccgaa
     3301 agacgaacta ccacggatct tcgagcgctt ctttcgcggg ggaaagccgc gggaaatgca
     3361 gttgcccggc accggcctcg gcctggcaat cgtgaaggat attgtcgacc tgcacggatg
     3421 gcgagtgacg gtagagagcc aggtgggcgg tggtagtaca tttacggtct ggatgccgct
     3481 cgcgtagagt gcaggatctg attgtctaaa caagggaagt gtatggacga gccccgtatt
     3541 ctgatcgtcg aggatcatga accactgcgg gtagcgatac agaccatttt ggaagcagaa
     3601 ggctggagcg tcctgagcgc gggggatggg ctagaggggc tcgaggtgat gagccaggtc
     3661 tgccctgact tgatcctggc ggatatcatg atgccgcgga tggacgggtt ggcattctac
     3721 ggtgcggtcc gcgccggcag ggagtggatg gccatcccct tcattttctt gactgccaaa
     3781 acgagccggg aagacgccct gaaagggata gaattggggg ccgaggatta catcaccaaa
     3841 cccttcgagc cggaagagct ggtggtcgcg gtgcgggcgc ggctgaagcg ggcggaggcc
     3901 cttcgggcag tgacagaggc tgagttcgac cgactcaggc agcagatcat cgccatcccg
     3961 cgtcaactcc tggaagcaca agaagccgag cggcgtagca tcgcgcgcga gctgcacgat
     4021 gagatcggcc aacttcttac cgggctcaga ttgatcctgg aaatggccgg acgcgagcta
     4081 cctggcgccc gagcggacag tctgacccag gcgcaggcgc tggtcggcga attgctggcg
     4141 cgtgtgcgcg agatgtcgct ccagctccgg ccgccgatgc tcgacgattt ggggttgctg
     4201 ccggcgctga tgtggcattt cgagcgctct gccgcgctga ctcaggtgcg cgtgaactac
     4261 aagcacatgg gcgtcgaaga ccgccgcttc ccgcaggaag tcgaaacggc cgcctatcgc
     4321 atcgcgcagg aagcgctgac caatgtcgcg cgccacgccc aggtgatcga ggtcaccgtt
     4381 cgcctttggg ctgtccagga aatgctcggc ctggaaatcg aagaccgcgg tgtggggttc
     4441 gatgccgctt ccgtgctcgc cacgcatagc tccagcggaa tttccggcat gcgtgaacgc
     4501 gcgtttctgc tgggcggtca cttcactgta gagtcagcgc cgggcaaagg cactcggatt
     4561 ctggtcgaat tgccgttcgg tggaaatact gtgagggaac agcatgagcg tgcgtattct
     4621 gcttgtggat gaccatccga ttgtgcggca gggcttgcgg actttgctgc aagccgaagc
     4681 gggatttgcc gtggtcggtg aggcgagcaa tgggctcgag gccctgagac tgatcgagcg
     4741 ctacaaaccc gatgtgctgg tcgttgatct gatgatgccc ggcgtgactg gcctggaggt
     4801 cgtgcaccaa accacacaac tctctcccaa tacgcgtgtg atcgtccttt cgatgcactc
     4861 ggacgaggcg tatgtgctgg aggcattgaa gagcggggcc atgggttacg ttctcaaaga
     4921 ggccaatccc accgatctga tccagggcgt gcacgaagta attgctggtc ggcgctatct
     4981 aagcgcacca ctctccgagc gcgccatcga ggtatacttc caaaggggcc aggaagccgg
     5041 agaagatccc tacgagacgt tgacgacgcg cgagcgcgaa gtgctgcagc tcgccgcaca
     5101 gggacttacg aaccatggga tcgctgcggc gctctccatc agttcgcgca ctgtagagat
     5161 ccatcgtgcg aacatgatgc acaagctcgg cttgcgtcat caggccgact tggtgcgtta
     5221 tgccgcgcgg cgggggctct tgcccttgga atgacgtggt tctccgtccg gtcgggtggt
     5281 caggcatagc tcaggatgag gaggtctccg cctctagacg cttacgccag tagcgcgata
     5341 gaccgtcgac ggacatgaaa tgcggattgg tcgccgcgta ggcctgaaat tcctcttcgc
     5401 cgacctgcca ggcgttcgcc cgcgcacggt cccaggccgt gtattcggcg atcaagtctt
     5461 cgcgagcggc cccggcctgc aggcgcgcgc gaacgaagcc tgccgcttcg tctaggagtc
     5521 tatccagcgt gtccaggtgc tctcgcacgt cgcttaccac gccaaaatgg gttggataga
     5581 ccgcagccaa tttttccgct gaaaggcgct cgaggctgc
//
LOCUS       DATCGS010000044         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_54185, whole genome shotgun sequence.
ACCESSION   DATCGS010000044 DATCGS010000000
VERSION     DATCGS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54185"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1458
                     /locus_tag="VIL39_00880"
     CDS             337..1458
                     /locus_tag="VIL39_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480010.1"
                     /translation="MTTIVPSPQPATVHHQPLDNAGTRRARLRFVPVIILWGLWLLLS
                     GGAAAQDITAFSGNGTLSWTNGLTNGAYEVQWSSSLTAGGAWTNDWSQLRGIQGTQSL
                     FTVPVPMFYRVKGSAYPEVTDSAECYARYSNALLNAAVALPEEISTRLRPVATNTPGT
                     DWRTFTNWVDGTTNRWIRVATMKFVGSWAWTSLLTPGAHTMSNGWSAETWVTLYPDAR
                     QLLAGYTGTNRTLRMEKALGLPPRPGAYGVAEFYVDPKYLFRPAASPDIHSPSCGLVA
                     EEASPYLAANALQGVSPGYAAWFKATFDSRGYAATNLDNSWPWTRLGYTCDYENSANS
                     PVGLSEYVVPSCSDTNYWGSGLVIPIYVDSTCQAANYGL"
     gene            complement(1592..>2956)
                     /locus_tag="VIL39_00885"
     CDS             complement(1592..>2956)
                     /locus_tag="VIL39_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480011.1"
                     /translation="LSATLLLATSLACAAEEAAQTLVVPDGKAYQGKKITAKTYNSGF
                     ALGHDSYNAMGTGSDGKIYYVLSSDNIDQGAKMFCFDPKTKRIKELGDLTEACGEKGS
                     KAIPQGKSHVNFIEANGKLYFATHVGVYSIVEGKECMGIPPAGYKAYPGGHLLSYDLK
                     TGQFEDFGICPGREGVLVFNMDTKRGHLFALTWPSGIFYRYDLATRNQKSFGKMCALG
                     EDGVGPDYRTVCRSIAVDQSTGAAYFTSSEGTIFRYDAATDTVAPVEGEDMKKDYFGL
                     YDPTNPGHMGYNWRQTLWRPSDKMIYGVHGNSGYLFRFDPRVPRIEVLDRITSEPSKR
                     CGMFDQFSYGYLGFALGPDGKTIHYLTGGPIYIGGKRVAGKSKTAMGEAKGLEDLHLI
                     TYDIETGRYADNGAVFYENGQRPLYVNAIAVGKDGMVYTLPRITENGKTRSDLVAFPG
                     PLKR"
BASE COUNT          534 a          884 c          939 g          599 t
ORIGIN      
        1 gtgcgtgatt gcggtggcgc gttgtattcc agacggttct acgacgttgt gaccttgccc
       61 taaccttcac ctgaggaagt ttggttcact ccaacgttca acaagacagg atgaaatcag
      121 cgggaacctg aaagtgacgg cggagacgac ggtatcggag tgcgcccgtc ctcgggcgca
      181 gcaagccccg aatgtgctga cgcgccggat ttcattatat ctctgccgct ttccaacatt
      241 gctgcgcccg aggacgggcc cactccgcca cgaccttctc ccgctgtcac tgacccatta
      301 cattcgaaat cacacatttg gattgaagca aaacacatga caacaatagt cccttcccca
      361 caacccgcga cggtccacca ccagccacta gacaacgcag gcacgcggcg agcgcggctg
      421 cgtttcgtcc cggtgatcat tctctggggc ctgtggctct tgctgagcgg cggggcggcg
      481 gcccaggata tcaccgcttt ctcaggcaac ggcactttga gttggaccaa tgggctgacc
      541 aatggcgctt acgaggtcca gtggtcgtcg tcgctgacgg ccggcggggc gtggaccaac
      601 gactggagcc aactgcgagg catccagggc acccaatccc tgttcactgt tccggtgccg
      661 atgttctacc gggtcaaagg cagtgcgtac cccgaggtga cggacagcgc cgagtgctat
      721 gcgcggtatt cgaacgccct gttgaacgcc gcggtggcgc tgccggaaga gatttccacc
      781 cggttgcggc ccgtggctac caacacaccg ggaactgatt ggcgcacgtt caccaactgg
      841 gtggatggca ccaccaaccg gtggattcgc gtcgcgacca tgaagtttgt cggcagttgg
      901 gcctggacca gcctgctgac gcccggcgct cacaccatga gcaacggctg gtcggcggaa
      961 acctgggtca ctttatatcc cgacgcgcga cagcttctgg ccggctacac cggaaccaac
     1021 cggaccttgc gcatggaaaa ggccctcggc ctgccgcctc gcccgggagc ctacggcgtg
     1081 gcagagtttt atgtggaccc gaaatacctc ttccgcccgg cggccagccc tgacattcat
     1141 tccccgtcct gcggactggt tgccgaagaa gccagcccct atctggccgc caatgccctg
     1201 caaggggttt ccccgggcta cgccgcctgg ttcaaggcta ctttcgactc gcgaggctac
     1261 gcggccacca acctcgataa cagttggccc tggacccggc tgggatacac ctgtgactac
     1321 gagaactccg cgaacagccc cgtggggctc agtgaatacg tggtgcccag ttgcagcgac
     1381 accaactact ggggatccgg cctggtaatc cccatctatg tggactcgac ttgccaggcg
     1441 gcaaattacg gcctgtgaaa gggtcgcata atctcccgat ccgttttagg atttgaatga
     1501 tgagctcgac cacggcccgg tgactacctg ggccgcagtt gagttgtctg agggagctta
     1561 ttcatgtggc cgttgcgatg gagcactcaa gctacctctt gagcggaccg gggaaagcga
     1621 ccaggtcgga gcgggttttg ccattctccg tgatgcgcgg cagcgtgtac accatgccgt
     1681 ccttgcccac agcgatggcg ttgacgtaga gggggcgctg gccgttttca tagaagacgg
     1741 cgccgttgtc ggcgtacctg ccggtttcga tgtcgtaggt gatcaggtgc aggtcttcga
     1801 ggcccttggc ttcgcccatc gcggtcttgg acttgccggc cacgcgcttg ccgccaatgt
     1861 agatcgggcc accggtcagg taatgaatgg tcttgccgtc cgggccgagc gcgaagccga
     1921 ggtagccgta gctgaactgg tcgaacatgc cgcaacgctt ggaaggctcc gaggtgatgc
     1981 ggtcgagcac ttcgatgcgc gggacacgtg ggtcgaaccg gaacaggtag ccggagttgc
     2041 cgtgaacgcc gtagatcatc ttgtcgctcg ggcgccagag ggtctgccgc cagttgtagc
     2101 ccatgtggcc cgggttggtg gggtcgtaca ggccgaagta atccttcttc atgtcttcgc
     2161 cttcgacggg cgcaaccgta tcagtcgcgg cgtcatagcg gaagatggtg ccttcggagc
     2221 tggtgaaata agccgcgccg gtgctctgat ccacggcgat ggatcggcag accgtgcggt
     2281 aatcggggcc gaccccgtct tcgcccaggg cgcacatctt gccaaagctc ttttggtttc
     2341 tggtcgccag gtcgtagcgg tagaagatgc cgctgggcca ggtgagggcg aacaggtggc
     2401 cgcgcttggt gtccatgttg aataccagca cgccctcgcg gccggggcag atgccgaagt
     2461 cctcgaactg gccggtcttg aggtcgtagg agagcaggtg gccgcccggg taagccttgt
     2521 agcccgcagg cgggatgccc atgcattcct tgccctcgac gatactatag acgccgacgt
     2581 gtgtggcgaa gtagagcttg ccgttggcct cgatgaagtt gacgtggctc ttgccctggg
     2641 ggatggcctt gctgcccttt tcgccgcaag cctcggtgag gtcgccgagc tctttgatgc
     2701 gcttcgtctt ggggtcgaag cagaacatct tggcaccctg atcaatgtta tccgagctga
     2761 gcacgtagta aatcttcccg tcgctgccgg tgcccatggc gttgtagctg tcatggccga
     2821 gggcgaagcc ggagttgtag gtcttggcgg tgatcttctt gccctggtaa gccttcccgt
     2881 cgggcacgac gagtgtctgg gcagcctcct cggcggcgca ggcaagcgac gtggctagga
     2941 gcagagtggc agagag
//
LOCUS       DATCGS010000045         5936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_350919, whole genome shotgun sequence.
ACCESSION   DATCGS010000045 DATCGS010000000
VERSION     DATCGS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350919"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1638
                     /gene="sufD"
                     /locus_tag="VIL39_00890"
     CDS             301..1638
                     /gene="sufD"
                     /locus_tag="VIL39_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY5480012.1"
                     /translation="MKETDPYLETFERFEAQAKQPAWIFPLRKAGIARFAELGFPTLQ
                     QEDWRFTNVAPIAKLPFKPVLQVSRHSLTREALTEFTFGKLAASRLVFVNGHYAAELS
                     SSPGPQPQGVIVGSLAAALASDSALIKEHLARYPQGEDNPFVALNAAFFQDGGFIHVP
                     AGKRLEAPVHLLFISTAKEAGATSHPRNLIIAEKGSQVTVLESYASLAEASYFTNAVT
                     EMILEEGAAVEHVKFQDESRNAYHVAALHAHLGKNSNFVSHSIATGARLSRNNIRTTL
                     AGEGVECILNGLYVTRGDQLADHHMVVEHAQPHCSSHEHFNGILDGHSKGVFHGRILV
                     QKLAQKTDAKQTNKNLLLSEDATVDTKPQLEIYADDVKCTHGATVGQLNEESIFYLRA
                     RGIGVETARRMLIHAFAGEIIERIRHITAREELNQLVWERLEQNPRVGEKKGV"
     gene            1631..2086
                     /locus_tag="VIL39_00895"
     CDS             1631..2086
                     /locus_tag="VIL39_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY5480013.1"
                     /translation="MFDDLSDLYQEVILDHCKQPRNFHELTGATCSAQGHNPVCGDRL
                     KVYLVMDGEVIKDISFLGSGCCISKASASLLTDAVKGKTRGEVQKMFELIHEMVMTGR
                     VVGDVGKLAVFAGVYKFPARVKCAILAWHAVMAALKGEGQPVSTENEAT"
     gene            2268..2816
                     /gene="sufT"
                     /locus_tag="VIL39_00900"
     CDS             2268..2816
                     /gene="sufT"
                     /locus_tag="VIL39_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEY5480014.1"
                     /translation="MSSDQPVTLTRDVEAAIIPAGTRVTLQKGEPTQITQTLGGSYTV
                     IVNGNMFRIEGKDADALGLEVAAKPVSTGAPVTQEQLEKGIWNQLRSCYDPEIPVNIV
                     DLGLIYDCHLTPLTTGSHRVDVKMTLTAPGCGMGPTLAQDVQSKVLSLEGVGDVAVEL
                     VWDPPWNQGMMTEAAKLQLGLL"
     gene            2825..4093
                     /locus_tag="VIL39_00905"
     CDS             2825..4093
                     /locus_tag="VIL39_00905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY5480015.1"
                     /translation="MITDAAIATKPEPAVNWSALRADFPILDQKVHGQPLIYLDNAAT
                     TQKPRAVIEALRHYYEHDNANVHRGIHELSNRATAAFEAARARAAQFINARSADEIIF
                     TRGTTEGINLVAQAWGSKNLKAGDRILLTEMEHHSNIVPWQLLAERTGAELIYLPITG
                     DIGLLDLNCLNECLAKKVKLFAMTHISNSLGTINPVADLCARARQLGVTTLVDAAQSA
                     GHRLINVQEIGCDFLALSGHKMCGPTGIGVLYGRQEVLESMPPYQGGGEMILSVGFQR
                     STWKDAPHKFEAGTPDISGAVGLHAAMDYLDNIGRLNITQHDQELGAYAYAQLSQFKQ
                     GIRLFGPHIGRAGLVSFLLKDVHAHDVVTVADQRGVALRGGHHCNQPLMRKLGVESTA
                     RASFFFYNTTAEIDRLVEVLGEIQKFFGAP"
     gene            4192..4494
                     /locus_tag="VIL39_00910"
     CDS             4192..4494
                     /locus_tag="VIL39_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480016.1"
                     /translation="MAMTRHCWKCGAKYELRGTPGRSESCPRCNSDLKVCLNCVSYDP
                     RVAEQCRERRADLVGEKQLANYCEYFEFIRREFVSPAEDTSREAKARQTLKNLLGD"
     gene            complement(4495..4998)
                     /locus_tag="VIL39_00915"
     CDS             complement(4495..4998)
                     /locus_tag="VIL39_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5480017.1"
                     /translation="MGFLAGATLTGCVINGFNAGANAGASADQTSWYIGGTINTPLKG
                     LKVGASYDYAGVSQQALSGANYANAMALYASCQATEKLSLHARGEYASSDALTSAGTP
                     LLGATKVVAATATPQYDLWKNVLSRIEFRWDHSASGSPAYGGEGAGAAPTKKNSFILL
                     ANVSYKF"
     gene            complement(4959..5393)
                     /locus_tag="VIL39_00920"
     CDS             complement(4959..5393)
                     /locus_tag="VIL39_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480018.1"
                     /translation="MALAGAGLVSLPTVICAEEKPNPVWTGLSSTTISSYIDTSAQWN
                     AGTGNANMPAYAFGGPGKADGFNLNVVELTLEKPIDTDQEWGAGYKVDLFLGPDAVTL
                     ATPSSGALSRPGPSHTRPTWDRSPSRRRRVWVSWPGRRLPAA"
     gene            complement(5497..>5936)
                     /locus_tag="VIL39_00925"
     CDS             complement(5497..>5936)
                     /locus_tag="VIL39_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5480019.1"
                     /translation="ATVATPVTGLRAGASFDFLDIHNAKNDTYSVAGYLSYQATEKLS
                     FHARGEYFKDKGWLVNGTDGVGDTVSTYNGALFDNVGDKGKAEVLAVTATVQYDLWKN
                     VISRLELRWDHSLLGNQLFGGTTQGDPGLKNAFMLGANVIYKF"
BASE COUNT         1240 a         1752 c         1814 g         1130 t
ORIGIN      
        1 gtgaggaaaa cgtgatgcgt gatgcgtgat gcgtgatgcg tgaagatggc ccaccacccc
       61 cgccggtccc ttttaccggt ttggctctat ggagctccgc tggacaacgc tgtcagcaac
      121 ctccgacaaa gcggcagcca aagaagttac atgagttaaa cgggttacat aagttacatg
      181 aggtcaagga gtttgcgggg gaacttgcgc gacatcccct tgcgtggaag actgtttgac
      241 tcaatttgac agacattttg agcaacacag actatggttc aactgatggt gaactcggca
      301 atgaaagaaa cggaccccta tctggagacg ttcgagcggt ttgaagcgca agcgaaacag
      361 ccggcgtgga ttttcccgct gcgcaaggcg ggcatcgctc gctttgccga attggggttt
      421 ccgactctcc agcaggagga ctggcggttc acgaacgtag cgccaatcgc caagctgccc
      481 ttcaaacccg tcctccaggt gtcgcgacac agcctcaccc gggaagccct gactgagttc
      541 accttcggca agctggccgc cagccggctg gtattcgtga acggccacta cgcagccgaa
      601 ctgtcgtctt cccccgggcc gcagcctcag ggcgtcatcg tcggcagcct ggccgcggcg
      661 ctggccagcg actcggcgct gattaaggaa catctggccc gctacccaca gggcgaagat
      721 aacccgttcg tggcgctcaa cgcggccttc ttccaggacg gcgggtttat tcatgttccg
      781 gcgggcaagc gtctggaggc gccggtgcat ctgctgttca tctcgacggc gaaagaggct
      841 ggggccacat cgcatccccg caacttgatc atcgccgaaa agggcagcca ggtgacggtt
      901 ctcgaaagct atgccagtct cgccgaggcg tcttatttta cgaacgcggt gaccgagatg
      961 attctggagg aaggggccgc ggtggagcac gtgaaattcc aggatgagag ccggaacgct
     1021 tatcacgtgg cggccctcca cgcccatttg ggcaaaaaca gcaacttcgt ctcgcattcg
     1081 attgccacgg gggcgcgcct gtcgcggaat aatattcgca ccactttggc cggcgaaggg
     1141 gtcgagtgca tcctgaacgg gctttacgtg acccggggcg accagttagc cgaccatcac
     1201 atggtagtcg agcacgccca gccgcattgc agcagccacg agcatttcaa cggcattctg
     1261 gatggccatt ccaaaggcgt ctttcacggc cgcatcctgg tgcaaaagct tgcacagaag
     1321 acggatgcca agcagaccaa caagaacctc ctgctgtcgg aagacgcgac ggtggatacg
     1381 aagccgcagt tggagatcta cgcggacgat gtgaagtgca cgcacggggc aaccgtcgga
     1441 cagttgaacg aagagtctat cttttacctg cgcgcccggg gcattggcgt ggagaccgca
     1501 cggcgcatgt taatccatgc cttcgcgggt gaaatcatcg aacgcatccg gcacattacg
     1561 gcgcgggaag agctgaacca gctcgtgtgg gagcggttgg aacagaaccc gcgcgtggga
     1621 gagaaaaaag gtgtttgacg atctaagcga tctttatcag gaggtcatcc tcgaccactg
     1681 caagcagccg cgcaatttcc acgagctgac tggcgcgact tgctcggcgc aaggccataa
     1741 tcctgtttgc ggcgaccggt tgaaagtgta cctggtcatg gacggggagg tcatcaaaga
     1801 catcagcttc ctcgggtccg gctgctgcat ctccaaagcc tccgcctcgc tgctaacgga
     1861 cgctgtcaaa ggcaagaccc ggggcgaggt tcaaaagatg ttcgagctga tccatgagat
     1921 ggtgatgacc ggccgggtgg tcggagatgt gggcaagctg gcggtattcg ccggcgtgta
     1981 caagtttccc gcgcgcgtca aatgcgcgat tctggcctgg catgctgtga tggcggccct
     2041 caagggcgag ggccagccgg tcagcaccga gaacgaggcg acctgacaaa tggcaagctc
     2101 tccctgtgtc ccggagcgcg gacattcctg tccgccgcag cactggtaat gaacaagcgt
     2161 gcgaacagcc gacatcgtga ggcgtccgga aggggctgcg gacaggaatg tccgcgctcc
     2221 aggcgacaac cgcagccgct tcggctatcc tttcattcgt tgataatatg agcagcgatc
     2281 agccagtcac tttaactcgc gatgttgagg ccgccatcat cccggccggc acccgggtca
     2341 ccctgcaaaa aggggaaccg acccagatca cccaaactct gggcggtagt tacaccgtga
     2401 tcgtgaacgg gaacatgttc cgcatcgaag gcaaggatgc ggatgcgctc ggtcttgaag
     2461 tggcggccaa acccgtcagc accggcgcgc cggtcacgca agagcagctc gaaaagggaa
     2521 tctggaacca gttgcgctcg tgctacgacc cggaaatccc tgtgaacata gttgatctcg
     2581 gcctgatcta tgattgccat ctcacgccgc tgaccacagg gagccatcgc gtggacgtga
     2641 agatgactct gacggcgccc ggctgcggca tgggccccac actcgcccag gatgtgcaga
     2701 gtaaggtgct cagcctggag ggcgtgggcg atgtggccgt ggagttggtc tgggacccgc
     2761 cctggaacca gggcatgatg accgaagcag ccaagctgca gttgggattg ctctaaacat
     2821 ggttatgatt actgacgcgg ccattgcaac aaagccagag ccagcggtta actggtcagc
     2881 cctccgggcc gactttccga tcctcgatca gaaggtgcac ggccagccct taatctacct
     2941 cgacaacgcc gccaccacgc aaaagccgcg cgcggtgatc gaggccctgc gccattacta
     3001 cgagcatgac aacgccaacg tccaccgcgg catccacgaa ttgagcaatc gcgccaccgc
     3061 cgccttcgaa gccgcgcgcg cgcgcgccgc ccagttcatc aacgcccgca gcgctgacga
     3121 gatcatcttc acccgcggca ccaccgaggg catcaacctg gtcgcgcagg cgtggggctc
     3181 gaagaacctc aaagccggcg accgtatcct gctcacggag atggaacacc acagcaatat
     3241 tgttccctgg caactgctgg ccgagcgcac gggtgcggaa ctcatttacc tgcccatcac
     3301 cggcgatatc ggcttgctcg acctgaactg cctaaacgag tgcctggcga agaaggtgaa
     3361 gctgttcgcg atgacccaca tctcgaactc gctaggcacg atcaatccgg tggcggactt
     3421 gtgcgcacgg gcgcgccagc ttggcgtgac gacgctcgtc gatgccgcgc aaagcgccgg
     3481 gcatcggctg atcaatgtgc aggaaatcgg ctgcgacttc ctcgccttgt cggggcacaa
     3541 gatgtgcggg ccgacgggca tcggcgtctt atatggccgc caggaggtgc ttgaaagcat
     3601 gccgccgtat caaggcggcg gggagatgat cttaagcgtc ggtttccaac gaagcacttg
     3661 gaaggacgct ccgcacaagt tcgaggccgg cacgccggat atttccggcg ccgtggggtt
     3721 gcacgccgcg atggattacc tcgacaacat cggacggctc aacatcacgc agcacgatca
     3781 ggaacttggc gcgtatgctt acgcccagct ctcccagttc aagcagggca ttcggctctt
     3841 cggcccgcac atcgggcgcg ccggactcgt aagttttctg ttgaaggacg tccacgcgca
     3901 tgacgtggtg accgtggccg accagcgggg cgtcgcgttg cgcggcggac accactgcaa
     3961 ccagccgctg atgcgcaagc tcggcgtcga atccaccgcc cgcgccagtt tcttcttcta
     4021 caacacgacg gcggagattg atcgcctggt ggaggtgctg ggcgaaattc agaagttctt
     4081 cggcgcgccc tgaaccctgt tagcgcgttg aagcgcgcca gagaccggtt tggcacgggg
     4141 agagtgaact ctccgctgga agtatcggcc tcggacgtta gaatgggccg catggcaatg
     4201 acgcgacatt gttggaagtg cggcgcgaaa tacgaattgc ggggaacacc aggccgttcg
     4261 gaatcgtgcc cccgctgcaa ctcagatttg aaggtgtgcc tgaattgtgt cagctacgac
     4321 ccgcgggtcg ctgagcagtg ccgtgaacgc cgcgccgacc tggtcgggga aaagcaactg
     4381 gccaactatt gcgagtattt cgagttcatc cggcgtgagt ttgtttcgcc cgccgaagac
     4441 acctcgcgcg aagccaaagc acggcagacg ctgaagaacc tgctcgggga ttaactagaa
     4501 cttgtagctc acgttggcca gcagaatgaa gctattcttc ttcgtggggg cggctccagc
     4561 cccttcccca ccgtaagcgg ggctgccaga ggcggaatgg tcccagcgga attcgatccg
     4621 gctcagcaca ttcttccaga gatcgtactg cggggtggcg gttgccgcaa cgaccttggt
     4681 ggcgcccaac aggggagtgc cagccgaggt taaggcatcg ctggaggcgt attccccgcg
     4741 ggcatgaagg ctcagctttt ccgtggcctg gcaggaggcg tacagggcca tggcattggc
     4801 gtagttggcg ccactcaggg cttgctggct cacgccggca tagtcgtaag aggcgcccac
     4861 cttcaagcct ttgagggggg tgttgattgt gccgccaata taccaactgg tttggtcagc
     4921 gctcgcaccc gcattggcgc ccgcgttgaa accgttgatc acgcagccgg taagcgtcgc
     4981 cccggccagg aaacccaaac tcttcggcgc cgtgagggtg aacgatccca ggtaggtctt
     5041 gtatgactcg ggcctggccg ggaaagcgcg ccggaggacg gtgtggccag cgtaacggca
     5101 tccggaccca gaaataaatc caccttgtat cccgcgcccc actcctggtc ggtgtcgatc
     5161 ggcttttcca gggtaagttc gacaacgttc aggttgaagc cgtccgcttt gcctggtccg
     5221 ccgaaggcgt aggctggcat gttcgcgttg ccggtgccgg cattccactg ggcggacgta
     5281 tcaatatagc tgctgatggt tgtcgaagaa agcccagtcc ataccggatt gggtttctct
     5341 tcggcgcaga taactgtcgg caggctgacc agtccggcac cggctaaggc caaagtccag
     5401 gcattacagt tcatcattcc agttgtctgc ggcccaaaca aaagccccct tccggttttc
     5461 ccggaagggg gcctgtgaga ttagttccag ttcagcttag aacttgtaga tgacgttggc
     5521 ccccagcata aatgcattct tcaatccagg gtcaccctgt gtggtgccac cgaacagttg
     5581 attgccgagc agggagtgat cccagcgcaa ttccaggcgg ctgatgacgt tcttccacag
     5641 atcatattgg accgtggcag tgaccgccaa gacttcagct ttgcctttat cccccacgtt
     5701 gtcgaagagg gctccgttgt aagtcgaaac tgtatcaccc accccatcag taccattaac
     5761 tagccacccc ttgtccttga agtactcccc acgagcgtgg aagctgagct tctcggtcgc
     5821 ttgatacgag agatagcccg cgacggagta ggtgtcgttt ttcgcgttat ggatgtcgag
     5881 gaagtcaaac gacgcacctg ctcggaggcc ggtcaccggc gtcgccactg tggcgc
//
LOCUS       DATCGS010000046         5237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_202252, whole genome shotgun sequence.
ACCESSION   DATCGS010000046 DATCGS010000000
VERSION     DATCGS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202252"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VIL39_00930"
     CDS             complement(<1..708)
                     /locus_tag="VIL39_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480020.1"
                     /translation="MFRNAPAKLVSLVPLFLVALSIHGEDLPPVAQWIPAEAPIMVEV
                     SEPLALLSPLLRPEFGKSVTAALDNGQPNLKLQQFQGVVASLELQLGTDWRTGLRRLA
                     GKGLSFSVGPSGATLLTADGEDEKLLSKLNGIIWQFAASEAETQNHPETVASNDYRGL
                     KIWTIGTNEVHTLLGPRLVLGNRTSFLHKVLDLRAGTGGKTLASSAVFKAAREAVGKE
                     AVASVFLNLQTLRETPGL"
     gene            1020..2441
                     /locus_tag="VIL39_00935"
     CDS             1020..2441
                     /locus_tag="VIL39_00935"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY5480021.1"
                     /translation="MLVSVVALLLATSRLWAADQLLPKVKIAKDGRSFATEAGNPFVP
                     LGVSYFRPGTGWAPQVWKQFDAEATRKDFARMKELGINCVRVFLSYGSFYSEPGVLKS
                     EGVEKFDQFLNLAEKAGIYVHPAGLDHWEGPPNWRPCVVGDQPTLQYTEQFWKLFAAR
                     YRGRHVIFAYDLRNEPMIEWQNQVMPPQWNAWLEKRYGAIEKVAAAWGGTNQLQFGKI
                     AVPRPRNALRNRELLDFQSFRGDLADEWTRRQVAAIKSADPAALVTVGFLQSSVPSVP
                     WGDSGDTGFRPERQAKLLDFLEIHFYPLVRGAYEYKSEDDELANLAYLESIVREVARP
                     GKPVVLAEFGWYGGAAKPTFDKGAHPIGTEEQQAKYLRRVVETSAGFVVGWLNWGFYD
                     SPGATDCSELTGLLRVDGTAKAWGKSFQELAAHYAGTRIAPVTIGPRPALDWDACLTS
                     SDSARQYREQYYKAFLDDKPRWK"
     gene            2474..4576
                     /locus_tag="VIL39_00940"
     CDS             2474..4576
                     /locus_tag="VIL39_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY5480022.1"
                     /translation="MNPRAFATLAVAAFACSALQAATFHVAPSGNDQNGGTQRKPFAT
                     LQRAQRAVRELKQSGKLDGPITVYLHSGTYWLQEPVVFGPEDSGTVSAPITYAAAPGE
                     RPVLSGGRPITGWRKFDDKLWAADLPDVKAGHWRFNQLYVGGEPRTRARIPNEGFLRV
                     AACPEGTPKTANYHKACQTFEFKPGDIRADWSNLQDVEVIVYHFWTDSHLPIKSVDTT
                     SNLVTFACKATKTFTDDFTEDGARYIVENVFEGLDAPGEWYLNRHTGVLFYYPKAGED
                     MGRVEVIAPRLPAFIRIQGNATQRHLVEHICFRGLSFHYSHFELPPGNSNGAQGSSSV
                     TAAVTLAGAHQCRFDQCEFKNLGTWAIEAGRGCAENSFTGNEVANVAAGGFRFNGGTE
                     RDHPLERDANNVITDNHLHHYGQVYPSAVGILLMNAEGTTVAHNHIHDGYYTGVSVGW
                     VWGYGRSVSQGNRIEYNHIHHIGQGLLSDMGGIYTLGVSPGTVIRNNLIHDVDANQYG
                     GWGIYHDEGSTHVLVESNVVYRTKFAPFNIHYAKEITVRNNIFALGRLEQLTRGRMEP
                     HKTVYFENNLVYWQTGELFKSNWKDEPHTFHLNPLNAGGTATLTSTFDMDWNLYFNPT
                     RKLEEVNFAGGTWSEWQARGKDVHSRYADPLFVDPEHGDFRLKPESPAWALGFQPIDL
                     SSVGPRTPASPPLAARAR"
     gene            complement(4601..4858)
                     /locus_tag="VIL39_00945"
     CDS             complement(4601..4858)
                     /locus_tag="VIL39_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480023.1"
                     /translation="MWCWLSQFLENPEASAPKPVPQTNTESTTVQPAKTEVNEGDIIP
                     GQRLESIRKIPQQTRLLYGGIAIGVLALGLVLARLFRRKTG"
     gene            4906..5124
                     /locus_tag="VIL39_00950"
     CDS             4906..5124
                     /locus_tag="VIL39_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480024.1"
                     /translation="MSSATEEIIRVCEALPPEKQTEVADFARFLLARKDDERWEQLLA
                     YPGPRPKLEAFLRESTAEPDEPLDPQRL"
BASE COUNT         1004 a         1709 c         1569 g          955 t
ORIGIN      
        1 caggccgggc gtctcgcgca gtgtctgcag gttcaggaaa acactcgcca cggcttcctt
       61 ccccaccgcc tcgcgagccg ctttgaacac ggccgacgac gcgagggtct ttccgcccgt
      121 gcccgcgcgc aggtccagca ccttgtgcag gaacgacgtt cggttgccca gcacgagtcg
      181 tggccccagc agcgtgtgga cctcgttggt gccgattgtc cagatcttga ggccccggta
      241 atcgttcgac gccaccgtct ccgggtgatt ctgcgtctcg gcttcggaag ccgcgaactg
      301 ccagataatg ccattcagct tgcttagcag cttctcgtcc tcgccatccg ctgtaagcag
      361 cgtagcgccc gaggggccga cgctgaagct caatcccttg ccggccaggc gacgcaaccc
      421 cgtgcgccaa tcggttccca attgcagctc cagcgaagcc accacgcctt ggaactgttg
      481 gagtttcaga tttggttgcc cgttgtccag tgcggctgtc actgacttgc cgaattccgg
      541 gcggagcagg gggctcagga gagccagcgg ctccgacacc tccaccatga tgggcgcctc
      601 ggcgggaatc cactgcgcca caggtggcaa atcctccccg tggatcgaga gggcaacaag
      661 aaacaggggc acgagggaaa ccagcttcgc aggagcattt cgaaacatat tcttacagat
      721 aatacggagt cgcaccccgg aaagttacaa aaaaggtaac tgttcaggtg ggagctcgga
      781 cattcttgtc cgctgcaacg cgtcccgcca atcggccggt aaatcgggaa tctctcggcc
      841 ctcggaagcc gctgcggaca agaatgtccg cgctccggcc tgaacagttg ccaaaaaagg
      901 gagccttgga accaaccggt ttgaagacat gctgcctgaa ttgaagaatt gaagaagatt
      961 gcttttctct gcgctctttg cgttctttgc ggttaatgcg cacgtttagc aaccacccca
     1021 tgttagtctc cgtggtcgcc ctgctgctgg ccaccagccg gctatgggcg gcggatcagc
     1081 ttcttccaaa agtaaagatc gccaaagatg gccgctcctt cgcgacggaa gccggcaacc
     1141 ccttcgttcc ccttggtgtg agctatttcc ggcccggcac cgggtgggcg ccgcaggtgt
     1201 ggaagcagtt cgatgccgaa gccacgcgca aggactttgc gcgcatgaaa gagctgggta
     1261 tcaattgcgt gcgcgtcttc ctcagttacg ggtcgtttta cagcgagccc ggggtgctga
     1321 agagcgaagg ggtggagaag ttcgaccagt tcctcaactt ggcggaaaag gcgggcatct
     1381 atgttcatcc cgccggtctc gaccattggg aaggaccccc caactggcgg ccgtgtgtgg
     1441 tcggcgacca gcccacgctg caatacacgg aacagttctg gaagctcttc gccgcccgct
     1501 accgcggtcg ccatgtcatc ttcgcctacg acctgcggaa cgagccaatg atcgaatggc
     1561 agaatcaggt catgccgccg cagtggaacg cctggctgga gaaaagatac ggggccatcg
     1621 aaaaggtggc tgccgcttgg ggcgggacca accagcttca gtttgggaag atcgccgtcc
     1681 cgcgaccccg gaatgcgctg cgcaaccgcg agctgctcga cttccagtcc ttccgcggag
     1741 acctcgccga tgaatggacg cgacgccagg tggcggcgat caagtccgcc gaccccgccg
     1801 cgctggtcac cgtcggcttc ttgcagagtt cggtcccctc cgtgccctgg ggcgacagcg
     1861 gcgataccgg cttccgcccc gagcggcagg cgaagctgct ggatttcctg gaaatccatt
     1921 tctatcccct ggtacgcggc gcctatgaat acaagagcga ggatgacgag ttggcgaacc
     1981 tcgcctattt ggaaagcatc gtgcgcgaag tcgcgcggcc cggcaaaccg gtggtgctgg
     2041 ccgagttcgg ctggtatggc ggcgcggcca agccgacgtt cgacaaaggc gcccatccga
     2101 tcggcaccga ggaacagcag gccaaatacc tgcggcgcgt ggtcgagact tccgcgggct
     2161 tcgtcgtggg ctggctcaac tggggctttt acgatagccc cggcgcgacc gattgcagcg
     2221 agttgaccgg tttgctgcgg gttgatggca ccgcgaaggc ctggggcaag agcttccagg
     2281 agctggccgc gcattacgcg ggcacgcgca ttgcgccggt gacgatcggc ccccggccgg
     2341 cgctggattg ggacgcgtgc ctcaccagct ccgacagcgc ccggcaatat cgtgagcagt
     2401 attacaaagc cttcctcgac gacaagccac gctggaagtg agtcaacttt tgtggcctgg
     2461 tctgaacatg cttatgaacc ctagagcttt cgccaccctt gcagtggccg ccttcgcctg
     2521 cagcgccctc caggctgcca ccttccatgt cgcgccctcg ggcaatgacc aaaacggcgg
     2581 cacgcagcga aagcccttcg ctacgctcca gcgcgcccag cgcgcggtgc gggagctgaa
     2641 gcagagcggc aagctcgacg gcccgatcac cgtgtatctc cacagcggca cgtattggtt
     2701 gcaggaaccg gtcgtgttcg gaccggaaga ttcgggcacg gtaagcgcgc ccatcactta
     2761 tgcggcggcc cccggcgagc gcccagtgct gagcggcggc cggcccatca ctggctggcg
     2821 gaagttcgac gacaagctgt gggcggccga cctgccggac gtcaaagccg gccactggcg
     2881 gttcaaccag ctttacgtcg gcggtgagcc gcgcacccgc gcgcgcatcc ccaatgaagg
     2941 ctttctgcgc gtcgccgcct gtcccgaagg cacgcccaaa accgccaact accacaaagc
     3001 ctgccagacc tttgagttca agcccggcga catccgcgcc gactggagca acctccagga
     3061 tgtggaggtg atcgtgtacc acttctggac cgattcccat ctccccatca agtcggtgga
     3121 taccaccagc aacctggtca cgttcgcttg caaggccacc aagacgttca ccgatgactt
     3181 caccgaggac ggcgcgcgct acatcgtgga gaacgtcttc gaggggcttg atgctcccgg
     3241 cgagtggtat ctcaatcgcc ataccggcgt cctcttctac taccccaaag ccggcgagga
     3301 catgggccgg gtcgaagtca tcgcgccgcg gcttccggcc ttcattcgca tccagggcaa
     3361 tgcgacgcaa cggcacctgg tggagcacat ttgtttccgc ggtttgagct tccattactc
     3421 ccacttcgag ctgcccccgg gcaactccaa cggcgcgcaa ggatcgtcca gtgtcaccgc
     3481 ggcggtgacg ctggcgggcg cgcatcagtg ccgcttcgac caatgcgagt tcaagaacct
     3541 cggcacctgg gccatcgaag ccgggcgggg ctgcgcggaa aacagcttta ccggcaacga
     3601 ggtggccaac gtcgccgcgg gcggcttccg ttttaatggc ggcacggagc gcgaccatcc
     3661 gctggagcgc gacgccaata atgtcatcac cgacaaccac ctccaccatt atggccaggt
     3721 ctatccttcg gccgtcggca ttctcctgat gaacgcggag ggcaccaccg tcgcgcacaa
     3781 ccatatccac gacggctact atacgggcgt gtcggtcggc tgggtctggg gctacgggcg
     3841 gagcgtcagc cagggcaatc gtatcgagta caaccacatc caccatatcg gccaggggct
     3901 gctctcggat atgggcggca tctacacgct cggcgtctcc ccgggcacgg tcatccgcaa
     3961 caacctcatc cacgacgtgg acgccaacca atacggcggg tggggcattt accacgacga
     4021 aggctccacg catgtgctgg tcgagagcaa cgtcgtctat cgcaccaagt tcgcgccctt
     4081 caacatccac tacgcgaaag aaatcaccgt gcgcaacaac atcttcgccc tgggccggct
     4141 cgagcagttg acccgcgggc gtatggagcc gcacaagacc gtctatttcg agaacaacct
     4201 cgtctattgg cagaccggcg agctcttcaa aagcaactgg aaggacgagc ctcatacctt
     4261 ccacctgaac ccgctcaatg ccggcggcac tgcgacgctc accagcacct ttgatatgga
     4321 ttggaacctc tacttcaatc ccacccgcaa gctcgaagag gtgaacttcg ctggcggcac
     4381 ctggagcgaa tggcaggctc gcggcaagga tgtccactcc cgctacgccg acccgttgtt
     4441 tgtggacccg gagcacggcg atttccggct caagcccgaa tcgcccgcct gggcgctggg
     4501 cttccagccc attgacctca gttcggtcgg tccgcgcacc cccgccagcc ctccgttggc
     4561 agcccgcgcg cgatgaggct catggggatg gggtccttgc ttagccggtt ttcctgcgga
     4621 agagccgcgc caggacgagc ccgagggcca acaccccaat ggcgatgccg ccatagagca
     4681 ggcgcgtttg ttgggggatc ttgcgaatgc tttccaaccg ctggccggga atgatgtcgc
     4741 cctcgttgac ttcggtcttg gcgggctgaa cggttgtgct ctcggtgttg gtctgcggga
     4801 ccggcttggg ggcggacgct tccgggttct ccaggaactg agagagccag caccacatct
     4861 cggtatatta ttgaatcaac caccgctttc agctaaatat caaccatgag ttctgctacc
     4921 gaagaaatca tccgcgtctg cgaggcgctg ccgccggaga agcaaaccga ggtcgccgac
     4981 ttcgcccgtt tccttctcgc ccgaaaagac gacgagcgat gggagcaatt actggcctac
     5041 cctggcccac gaccgaaact ggaagctttc ctccgcgaat ccaccgccga gcctgacgaa
     5101 ccactcgacc cgcaacgcct gtgaaatccc gcgcccggcc cagcttctgg cgcgcttacg
     5161 gctcgctgga tccccgtgtc cgtgaggctg cgcgccgcgc ttatcggctg tttctggaca
     5221 atcccagcca ccccgct
//
LOCUS       DATCGS010000047         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_252114, whole genome shotgun sequence.
ACCESSION   DATCGS010000047 DATCGS010000000
VERSION     DATCGS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252114"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1110
                     /locus_tag="VIL39_00955"
     CDS             217..1110
                     /locus_tag="VIL39_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5480025.1"
                     /translation="MNLNRRKFLTLSALTGIVGAIPPTVQALTLPEPSPKARLRLSCQ
                     DGVAPGKTIAEKLDFLEANGFEAFEPHGKDLPKTADELQKALQGRKIKVGVVCGGMQG
                     CLISDQEEERRKGMESLKEVLTAAGALGATGVIMVPARPNQTKLGHWEARELLVKSLP
                     ELGDHAQKAGLHLLLEPLNRRECHFLRQVADGAAICRDVNHPGIRVMGDFWHMTWEET
                     SDMAAFIAGGQYLRHVHIASRKTRKMPGEDAGDNYVNGFKGLKLIGYQDMISFECGLK
                     GERRSALIAAAKLLREQWESA"
     gene            1241..2638
                     /locus_tag="VIL39_00960"
     CDS             1241..2638
                     /locus_tag="VIL39_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480026.1"
                     /translation="MTAKAPAQQPPAKPHPANGNLPSPSPSIKRRQFLKSAAFVGASG
                     LILPRFKLFGAEAPSNKLNIALIATGHRAQDHFAGASRENVVALCDVNEQHLASTARK
                     FPQAKLYADWRKCLEQKDIDAIICCTLDHTHAFVTNWAMNRGKHVFCEKPLANSIEEA
                     RALRATWLKNKDKLATQVGTQRHQSPNFDRVREMIRDGAIGKLEEVSAWGNRKIPRPG
                     YLPSEGEPPSYINYDLWLGPSPFHPYNSEYFKGGCLRWNMHWDWGTGQVGDMGCHTMD
                     LAWNAIDAGLPTSAEGKGDPFNPDVTPVKLETRFDIPGNDWRSAIKVSWYQGGALPES
                     PHPGIDLDKIGHGVLFEGSSGCLVADFQSRFLMPRGNQSDLTYYKPRGKAEQIPPRGN
                     FQQEWIAACKGSLKTSCDFGYASDMIEMMLLGLVAYRAGRKIKYDGATGRVTDCPEAN
                     ELLRHTYRPGWTLNG"
     gene            2978..3268
                     /locus_tag="VIL39_00965"
     CDS             2978..3268
                     /locus_tag="VIL39_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480027.1"
                     /translation="MSTSEMVAEKVKGLDEFQAQAVLTFIQELSTAPVLSATELMRLP
                     PAERRRILASQARQAEALYRQNPEMIVEDGDAPITAVQVEQVAAVIALCVGY"
BASE COUNT          711 a         1045 c          964 g          622 t
ORIGIN      
        1 ccaaccgttg caatctgagg cacgtagcgc caaaaccgct ccagaaaacg ctatatgtgg
       61 ggttctccga aagcggccgg ccactacagg tagtgtgctt tggctggtcc caattcccaa
      121 tcccagaagc gcgccgaccc ctgctatggc cgttcgatct caggcagaaa ccgctatcgc
      181 tttacccagc ctagccccat aatctgacgt aagcttatga acctgaatcg ccgcaagttt
      241 ctaactctgt ccgcgctgac tggcatcgtc ggcgccatcc cacccaccgt ccaagctctg
      301 actctgcctg aaccatcacc gaaggcgcgg ttgcgcttgt cgtgccagga tggcgtcgcg
      361 cccggcaaaa ccattgccga aaagctcgat ttcctggagg ccaacggctt cgaagccttc
      421 gagccgcatg gcaaggattt gccgaaaacc gccgacgaac tgcaaaaggc gttgcagggt
      481 cgcaagataa aggtaggcgt cgtttgcggg ggcatgcaag gttgccttat ttcggaccag
      541 gaagaagaac gccgcaaagg tatggagagc ctgaaagaag tgctaaccgc tgccggcgcc
      601 ttgggtgcca ccggggtaat catggtgccg gcccgcccca atcaaaccaa gctcggccac
      661 tgggaagccc gcgagctcct cgtcaagagc ttgcccgaat tgggcgatca cgcccaaaaa
      721 gccggcctcc acctcctgct cgaaccgctc aatcggcgcg aatgccattt cctgcgccag
      781 gtggccgacg gcgccgctat ttgccgggac gtcaatcatc ccggcatccg cgtgatgggt
      841 gatttctggc acatgacgtg ggaggaaacc agcgatatgg ccgcctttat tgccgggggc
      901 cagtatcttc gccacgttca catcgccagc cgcaaaactc ggaaaatgcc cggcgaagac
      961 gcgggcgaca attacgtgaa cggcttcaaa ggcctcaagc tcatcggcta ccaagacatg
     1021 atctcctttg aatgcggcct caaaggtgag cgccgcagtg ccctcatcgc cgcagccaaa
     1081 ctcctccgcg agcagtggga atccgcctga acaagttagg ctatcggggt tgatgaatat
     1141 ggacccgctt gcgaaaacgc gtccttccaa cggggaagtg ttgtcggtcg cggtaatttt
     1201 actcttgagt ctgccacgta gcttcgggaa acttctgtcc atgacagcaa aggcacctgc
     1261 acagcaaccg cctgcgaaac ctcacccggc aaacggcaac ctcccgagcc cgtccccgtc
     1321 aatcaaacga cgccagttcc tcaagtccgc cgcctttgtc ggagcgtcgg gactaatcct
     1381 cccccgcttc aaactctttg gggcagaggc cccgagcaat aagctaaaca tcgccctgat
     1441 cgccaccggg caccgcgcgc aagatcattt cgccggggca tcccgcgaaa acgtggtcgc
     1501 cctctgcgac gtcaatgagc agcacctggc ttccaccgcc cggaagtttc cccaggcgaa
     1561 gctttatgcg gactggcgca agtgcctgga gcaaaaggac attgacgcca tcatctgttg
     1621 cactctggat cacacgcatg cctttgtcac gaactgggcg atgaaccgcg gcaaacacgt
     1681 cttttgtgaa aagccgctgg ccaacagtat cgaggaagcc cgagcccttc gcgccacttg
     1741 gctcaagaac aaagataagc tcgccaccca ggtgggcacg cagcgccatc agtcgccaaa
     1801 cttcgatcgg gtgcgtgaga tgatccgcga cggcgccatc ggcaaactcg aagaagtgag
     1861 cgcctggggc aaccgcaaga ttccccggcc cggctatctg ccctctgaag gcgagccgcc
     1921 cagctacatc aattatgatt tatggctggg tccctcgccg tttcatccct acaactcgga
     1981 atatttcaag gggggctgcc tccgctggaa catgcattgg gactggggca ccggccaggt
     2041 aggcgacatg ggctgccaca ccatggacct ggcgtggaac gccattgacg ccggcctccc
     2101 aacctccgcc gaaggcaagg gcgatccgtt caatcccgac gtgaccccgg tcaaactgga
     2161 aacgcgtttt gatatcccgg gcaacgactg gcgctcggca atcaaggtgt cgtggtatca
     2221 aggcggcgcc ttgccggagt ccccgcatcc cggcattgac ctggacaaga ttggtcatgg
     2281 ggtgctgttc gagggatcga gtggctgcct ggtggccgat ttccagtctc gctttctcat
     2341 gccccggggg aaccagtcgg acctgacgta ttacaagccc cgcggcaaag cggagcagat
     2401 tccgcctcgg ggtaatttcc agcaggaatg gatcgcggct tgcaagggca gcctcaagac
     2461 ctcctgcgac ttcggctatg ccagcgacat gattgaaatg atgttgctcg gcctggtggc
     2521 ctatcgcgcc ggccggaaga tcaaatacga cggcgccacc ggacgcgtca ccgactgccc
     2581 tgaagccaac gaattactcc gtcacactta ccggccgggc tggacgttga atggttagca
     2641 gcccattgaa ctcgatttct tgccgctgaa ataggatcca gaccggcttt ttgggtgggg
     2701 cggattgttc ggctaccgcc cgactgccga gcgaaacgac cctgccgacc aagtggattg
     2761 cagcgcgggt gcagatcggc acgaccaaag gggccaagtc cgtgctccct catctggctc
     2821 agagccaaga ccaacgccaa accgcaagcg ccctcgaacc ctgcgcccgg ctcgaattct
     2881 aatctacggt ttgacccgga acatgctcaa atgtaccttg cgctgcgcgg gtgatcccgt
     2941 ccttgcatat cattcggcgc tcgcttaggt tctcggcgtg agcacgagcg aaatggtcgc
     3001 ggaaaaggtg aaaggcctgg atgagtttca ggcccaggcc gtcctgacgt tcattcaaga
     3061 gctttccacc gcgccggttt tgagcgccac ggagttgatg cgtctgccgc cggcagaacg
     3121 gcggcgcatt ttggcgagcc aggcccggca ggcggaggcg ctctaccgcc agaatcctga
     3181 gatgatcgtg gaggatggcg acgcgcctat cacggccgtg caggtcgagc aagttgcggc
     3241 ggtcattgcg ttgtgcgtcg gatattgaag cggggactgt cattcagttc cgcggcaaaa
     3301 cgacgagcgg cggcggcaag cgaagcgcga cgccgctttc cc
//
LOCUS       DATCGS010000048         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_351275, whole genome shotgun sequence.
ACCESSION   DATCGS010000048 DATCGS010000000
VERSION     DATCGS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351275"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2354
                     /locus_tag="VIL39_00970"
     CDS             <1..2354
                     /locus_tag="VIL39_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY5480028.1"
                     /translation="DLLLLAPVVRNRKGFHTDVAEWAASHGYAEVRADGKFYPTNERL
                     RLDRFREHDVEIVVGVLEAKHRGKGLHESNGLNQLHKAGNARRDGDSAPCLEAQQLID
                     EALKLGQGTLFALDNHKHTTVHSTERACPKCGRSFEALDPKNFSYNSPQGWCPRCRGF
                     GELFYLPDVDRGARAEDIEESWYSWQEGEREICSDCGGARLNPLARAVRLGMGNGQSP
                     MANWKAAPTIDSFGQMPVAAAYDFFRRVVFRGRAALIARDILPEIRERLKFLGEVGLG
                     YLQLGRGVPTLSGGEGQRIRLAAQLGSNLSGVLYILDEPTIGLHARDNEQLLAALQKL
                     KARGNSVLVVEHDEATMRRADYIIDLGPGPGVHGGEVVATGTLAELLRHPESVTGKCL
                     RAHRSFPTRGKRRTVGRGEVQSPRSKVQSPKSEVSPSLPASAPAVQEEERWLTLSGAS
                     KNNLKNLTVRFPLGRLVLVTGVSGSGKSTLIRECLLPALKSAITHPPSRITDHASRIT
                     GFDSIQAVYEVDQSPIGRTPRSIPATYVGFFDDIRQLFAQVPEARLRGYSPSRFSFNS
                     AQGRCPECEGAGMIKLEMNFLPPAFVRCEVCDGTRFNRETLDIEYHGKNVAAVLDMSV
                     EEALDFYASLPKIRRPLQALSDTGLGYLKLGQTSPTLSGGEAQRVKLVSHLLAGLKEP
                     HLLDRLPKRNVFILEEPTIGLHMADVQRLAEVLQRLVDAGHSIIVIEHNLDLIAEADW
                     VIDLGPEGGEGGGRIVVEGTPEQIARNKRSHTGRFLRSVLAKA"
     gene            2420..3589
                     /locus_tag="VIL39_00975"
     CDS             2420..3589
                     /locus_tag="VIL39_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY5480029.1"
                     /translation="MRAPNIVPIVCAGFMLLAQFTGGCVDSRPAPSQPLPNHQKIMGT
                     AARTNAALDWIKVAKDKHGFVCAASGQQFIPWGFNYDRDYKFRLLEDYWETEWPTVVE
                     DCREMKQLGANVVRVHLQFARFMAAPDRPNEEALDHLGRLVKLAEETKLYLDLTGLAC
                     YRKQDVPAWYSALGEAERWEAQARFWEAIAGRCAGSPAIFCYDLMNEPIVPAGARKPG
                     DWLTGELAGFNYCQFISLDQAGRPRPEIARRWATRLAAAIRKHDPRHLVTVGLLPNSL
                     AGSAWASGFEPRRIAGKLDFISVHLYPKTGHLEEDLKTLRGFKIGKPVVLEETFPLGC
                     SAADLGDFIGRSKRYAAGWIGFYWGQTPAELSKSTNIGDALTAAWLKVFQEMKPR"
     gene            complement(3642..4151)
                     /locus_tag="VIL39_00980"
     CDS             complement(3642..4151)
                     /locus_tag="VIL39_00980"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY5480030.1"
                     /translation="MNRIEVAEGDITKQVVDAIVNAANTTLLGGGGVDGAIHRTAGPE
                     LLEECRTLRGCATGQAKITKGYRLPAKWVIHTVGPVWRDGRHGEDDLLASCYRSCLAL
                     AEEHGISTIAFPSISTGAYGFPMERAARIAVRETKKFLQENPAVKKVLLVCFGKSAYD
                     MHASAVKEG"
BASE COUNT          753 a         1258 c         1371 g          775 t
ORIGIN      
        1 gcgacctgct cttgctggcg cccgtggtga ggaatcgaaa gggcttccat accgatgtgg
       61 ccgaatgggc cgccagccac ggttatgcgg aggtccgggc ggacgggaaa ttctatccca
      121 ccaacgagcg cctgcgcctg gaccggtttc gagagcatga cgtggagatc gtggtggggg
      181 tgctcgaggc gaaacaccgg ggaaaggggt tacatgagtc aaatgggtta aatcagttac
      241 ataaggcggg aaacgcgcgg cgcgatgggg acagcgcgcc ctgcctggag gcgcagcagc
      301 ttattgacga ggcgcttaag ttggggcagg gcacgttgtt cgccctggat aatcacaagc
      361 acaccacggt ccattccacg gaacgcgcgt gcccgaagtg cggtcgctcg ttcgaggcgc
      421 tcgacccgaa gaatttcagc tacaactccc cgcagggctg gtgcccgcgc tgccgcggtt
      481 tcggcgagct gttctatctg ccggacgtgg atcgcggcgc gcgcgcggaa gacattgagg
      541 agtcgtggta tagctggcag gagggggagc gggagatttg ttccgactgt gggggggcaa
      601 ggctgaatcc gctcgcgcgg gcggtcaggc tggggatggg caatggccaa tcgccaatgg
      661 caaattggaa ggccgcgccg acgattgact cgttcggcca gatgccagtc gcggcggcgt
      721 atgacttctt tcggcgcgtc gtgtttcgtg gtcgcgcggc gctgatcgcg cgcgatatcc
      781 tgccggagat tcgggagcgg ctgaagttcc tgggcgaagt cggcctgggc tatttgcagc
      841 ttgggcgtgg tgtccccacg ctgtccgggg gcgaggggca gcgtatccgg ctggccgcgc
      901 aattgggctc caacctgagc ggcgtgctat acatcctgga cgagcccacc attggcctcc
      961 acgcccgcga taatgagcaa ctgctggcgg cgctgcaaaa gctcaaggcg cgcggcaact
     1021 ccgtgctcgt ggtcgaacac gacgaggcga ccatgcggcg cgcggattac attatcgatc
     1081 tgggcccggg gccaggggtg catggcggtg aggttgtggc cacgggcacg ctggccgagt
     1141 tgctgcggca cccggaatcg gtcaccggga aatgcctgcg ggcgcatcgg agctttccta
     1201 cgcgagggaa gcggagaacg gtggggcggg gcgaagtcca aagtccaagg tccaaagtcc
     1261 aaagtccgaa gtccgaggtt agtccgagcc tgcccgcgtc ggctcctgcg gtgcaggaag
     1321 aggagcggtg gctgacgctt agcggcgcga gcaagaataa cctaaagaac ctgacagtgc
     1381 ggtttcccct gggccggctg gtgctggtga cgggagtaag cggctccggc aaaagcacac
     1441 ttatccgcga atgccttctc cccgcgctca aatcggccat cacgcatccc ccatcccgga
     1501 tcacggatca cgcatcacgc atcaccggct tcgactccat ccaggccgtg tatgaagtgg
     1561 accagtcccc gattggccgc accccgcgct cgattccggc cacctatgtt gggttctttg
     1621 acgacatccg ccagttgttc gcgcaggttc ctgaagcgcg gctgcgcggc tattcgccca
     1681 gccgattctc cttcaacagc gcgcaaggcc gttgccccga gtgtgagggc gccggcatga
     1741 tcaaactgga aatgaacttc ctgccgcccg ctttcgtgcg ttgcgaagtc tgcgacggga
     1801 cgcggttcaa ccgggagacg ctcgatatcg agtatcacgg caagaatgtt gccgcggtgt
     1861 tggatatgtc ggtggaggaa gcgttggact tctatgcgag cctgccgaag atcaggcggc
     1921 cgttgcaggc gctgtccgat accggcctgg gttatcttaa gctcggccag accagtccca
     1981 cgctcagcgg cggcgaagcg cagcgggtga agctggtcag ccatctgctg gcgggcctga
     2041 aagagccgca cttgctggac cgcctgccga agcggaacgt gttcatcctc gaagaaccga
     2101 ccatcggttt gcacatggcg gacgtgcagc ggttggctga ggtgcttcag cggttggtgg
     2161 atgccggcca ttcgataatc gtcatcgagc ataacctcga cctcattgcc gaagccgatt
     2221 gggtcattga cctcggcccc gaaggcggcg agggcggcgg gcgcatcgtg gtcgagggca
     2281 cgccggagca aatcgcgcgc aacaaacgct cgcacacggg acgcttcctg cgtagtgttt
     2341 tggcgaaggc atgagcgcga cgtatgcgga gacccgcgtt gagggccggg cgcctggccc
     2401 cggccaagtg atgttcgcaa tgagagcgcc aaacatagtt ccaatcgtct gcgccggctt
     2461 catgctgctg gcccagttta caggcggctg cgtggactct cgacccgccc cttcacagcc
     2521 gctacccaac caccagaaga tcatgggcac tgccgcgcgg accaacgccg ccttggattg
     2581 gatcaaggtc gcgaaggaca agcatggttt tgtgtgcgca gcctccgggc agcaattcat
     2641 cccctggggt ttcaactatg accgggatta caagttccgg ctgctggagg actactggga
     2701 aaccgagtgg cccacagtcg ttgaggattg tcgtgagatg aagcaactcg gcgccaacgt
     2761 ggtgcgggtt caccttcagt tcgcccggtt catggctgcg ccggaccggc ccaatgagga
     2821 agcgctcgac cacctcggcc ggctcgtcaa gctggcggag gagacaaagc tgtacctcga
     2881 tctgacaggg ctggcctgct accgcaagca agacgttccc gcctggtaca gcgctctggg
     2941 tgaagctgag cgctgggaag cacaggcgcg tttctgggag gcaattgccg ggcgttgcgc
     3001 cggcagcccc gccatcttct gttacgacct catgaacgag ccgatcgtcc cggcaggagc
     3061 gcgaaagccc ggcgactggc tcaccgggga gttggccggg ttcaactatt gccaattcat
     3121 ctcgctggat caggctggtc gtccccgtcc ggaaattgcg cgtcgttggg ccactcggct
     3181 cgccgctgcc atccgcaagc acgacccgcg gcatctggtc accgtcggcc tgctgcccaa
     3241 tagcctggca ggctccgcgt gggcgtcggg ctttgagcct cgcaggatcg ccggcaagct
     3301 cgatttcatc agcgtgcacc tttatccgaa gacagggcat ctggaggagg acctgaagac
     3361 cctgcggggg ttcaagatcg gcaagcccgt ggtgctcgag gaaaccttcc cactcggctg
     3421 cagtgcggca gacctcgggg acttcattgg ccgttccaaa cggtatgccg ctggctggat
     3481 tggattctat tgggggcaga cgcccgcgga attgagcaag tcaaccaaca taggcgacgc
     3541 cctcacggcg gcctggctga aggtctttca ggagatgaag cccaggtgag ccccgtgcgg
     3601 ggccgctttt tcccgatacg accgagggcg tcggttccct ctcaaccctc cttcaccgcg
     3661 ctcgcatgca tgtcataggc actcttgccg aagcagacga gcagcacctt cttcactgct
     3721 ggattctcct gcaggaactt ctttgtctcc cttaccgcaa tgcgagcggc ccgttccatc
     3781 gggaagccat aggcgccggt actgatggag gggaaagcga tggtgctgat accatgctcc
     3841 tcagccagcg caaggcagct tcgatagcag cttgcaagca gatcatcttc gccgtgccgg
     3901 ccgtcgcgcc agactgggcc gaccgtatgg atgacccatt tggccggcag ccggtagccc
     3961 ttggtgattt tcgcctgccc ggtcgcgcag ccgcgcagcg ttcggcactc ttccagcagc
     4021 tccggtccgg cggtgcgatg aatcgcaccg tccacgccgc cgccgcccag caaggtcgtg
     4081 ttcgcggcgt ttacgatggc gtccaccacc tgcttggtga tgtcgccttc cgccacttca
     4141 attcggttca agctcat
//
LOCUS       DATCGS010000049         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_252148, whole genome shotgun sequence.
ACCESSION   DATCGS010000049 DATCGS010000000
VERSION     DATCGS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252148"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VIL39_00985"
     CDS             <1..514
                     /locus_tag="VIL39_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5480031.1"
                     /translation="ADNLKQLGLAFRTRAINGGAAFSAEASTNREVALGSTTSGEAFR
                     YFQVMSNELGNPKLLVCPTDTREPAKDLGPGFSNANLSYFMSLDAEETYPGMFLSGDR
                     NLTNGLPIQDGILVLMPNRPLGWTHELHKGQGNVALADGSVQGFTSSRLGSGTIGPLV
                     AGMTNRLAMP"
     gene            590..1801
                     /locus_tag="VIL39_00990"
     CDS             590..1801
                     /locus_tag="VIL39_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5480032.1"
                     /translation="MKRLLPLSLTLLLAAGCSSIKTTERHTGPWNMASLKQAPTAEWG
                     GRTGLVQEVYYQGEPYRGKATRVFAYLGRPAAGKGPFPALVLVHGGGGNAFRAWAEHW
                     ASRGYVALAMDTSGCGPNGPLPDGGPDQSDTTKFRDFTDEEASDMWTYHAVSAVNRGV
                     SLLAALSEVDRRRIGITGISWGGYLTCIVAGLDDRLKVAVPVYGCGFLGENSCWKTTS
                     LAKMRPEARDRWLRNFDPSQYLGGVNCPILFLNSATDFAYPLDSYRACYRLVPSRFRH
                     VCVVLNLPHGHIWTFNEVDVFVDSVLRGGPPLPCFEQPKIDGDRVSVKVVSPVPVKEA
                     ALHYTTDSGEWQKRHWQTLPAELKDNTIIARLPGQRPLVWYLAVTDERGLRVSTEHEE
                     LPAAAHIKVAE"
     gene            complement(1951..>2861)
                     /locus_tag="VIL39_00995"
     CDS             complement(1951..>2861)
                     /locus_tag="VIL39_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY5480033.1"
                     /translation="HQYEFARLSLGYTVMSKRKLMQLVNENLVSGWDDPRMPTISGLR
                     RRGVTQEALRDFAYNIGITKYNGITDVSVLEYCIREDLNHRAQRRLAVLRPLKVVITN
                     YPEGQVEELEAINNPEDPNAGKRKLPFSRELYIERDDFMETPPPKYFRLRPGGEVRLK
                     YAFIIKCDEVVKDAAGQVVELRCTADLGSKSGGPTSGRKVKGTIHWVSAAHAIDAEVR
                     VYDRLFTVPEPDAGGDFKKHLNPHSLEVVTAKCEPSLKDARADVRYQFERLGYFTLDK
                     GSAPGKLVFNRTITMKDAWAKEAQKK"
BASE COUNT          517 a          857 c          906 g          581 t
ORIGIN      
        1 cgcggacaac ctgaagcagc ttggcctggc tttcagaacc cgggcgatca atggcggggc
       61 tgcgttttct gcggaggcat caacaaaccg ggaagtagcg cttggaagta cgacaagtgg
      121 cgaggccttc cgctatttcc aagtcatgtc gaatgagttg ggcaatccaa aactcctggt
      181 ttgcccgacc gatacccgtg agccggccaa ggatctcggc cctggattct cgaacgccaa
      241 tctcagctac tttatgagcc tggacgccga ggaaacttat cccgggatgt tcctatctgg
      301 agaccgaaac ctcacaaacg gcctgcccat ccaggatggc atactggttc tcatgcccaa
      361 tagacccttg ggttggactc acgagcttca caaaggccag ggcaatgtgg cgttggccga
      421 tggcagcgtt cagggattca ccagctcgcg cctagggtcg ggtacgatcg ggccgcttgt
      481 agcaggcatg acgaaccggc tggcgatgcc gtgactggtt cttatggctg gcaccaagga
      541 cattcttgct ttgctgccga ggcgcaggcg gggtaagcaa ccgggcgcaa tgaaacgtct
      601 attacccctt tcgctaacgt tgctgctggc ggccgggtgc agttcgatca agacaaccga
      661 gcgtcacacc ggcccgtgga acatggcttc tctcaagcaa gcgccaacgg ccgaatgggg
      721 cgggcggact gggctcgtcc aggaggtcta ttaccagggc gaaccctatc gaggaaaagc
      781 gacccgtgtc tttgcttacc tcggccggcc ggccgcaggc aaaggaccgt tccccgcgct
      841 ggtcctcgtg catggcggag gcggcaacgc cttccgagcc tgggctgagc attgggccag
      901 tcgcggctat gtggccctgg cgatggacac gtcgggctgc ggtcccaacg gccccttgcc
      961 agacggcggg ccagaccaga gcgacactac caagttccgc gacttcaccg acgaggaggc
     1021 aagcgacatg tggacttatc acgctgtcag cgccgtgaat cggggcgtgt cgttgctggc
     1081 cgcgctgtcg gaggtggacc gtcgccgaat cggcatcacc ggcatcagtt ggggcggcta
     1141 cctgacgtgc atcgtcgcgg gccttgatga ccggcttaaa gtggcggtgc cggtttatgg
     1201 ctgtggcttc ctgggagaga acagttgctg gaagacaacc tccctagcga agatgcgccc
     1261 ggaagcccgc gaccgctggc tacggaactt tgacccgtcg cagtacctcg gcggcgtcaa
     1321 ttgccccatc ctgtttctga atagcgccac cgatttcgcc tatccgctcg actcgtatcg
     1381 cgcctgctat cggctggtgc cgtccaggtt ccgccatgtc tgcgtggtgc tcaatttgcc
     1441 ccacggtcac atctggacct tcaacgaggt ggatgtattc gtggacagcg tcctgcgtgg
     1501 tggccctccc ctaccctgtt tcgagcagcc gaagatcgac ggcgaccgtg tgagcgtgaa
     1561 ggtcgtctca cccgtgccag tcaaagaagc cgccctgcat tacacgaccg actcgggtga
     1621 atggcagaag cgccactggc agacgttgcc ggcggaattg aaagacaata ccatcatagc
     1681 tcgtttgccc gggcaacgcc cgctggtgtg gtatctggca gtcaccgatg agcgcgggct
     1741 tcgggtaagc accgagcacg aggaattgcc tgccgccgca cacattaaag tggcagagtg
     1801 agcctgcggt tggtgtccgg cgggtgggat tggattacac cccattcccc cagcatcaca
     1861 cacgcacgcg acaccgagac ccgtgagcac tgaggcgcag ccgtttcccg tcttaaatcg
     1921 caggattcgc cccagcttgc tctcgcttgc ttacttcttc tgggcctctt tggcccaggc
     1981 gtccttcatc gtgatggtcc ggttgaagac cagcttgccg ggggcgctgc ctttatcgag
     2041 tgtgaaataa ccgagccgct cgaactggta gcggacatct gcgcgcgcgt ccttaaggct
     2101 cggttcgcac ttggcggtta ccacttccag ggagtgcggg ttgagatgct tcttgaagtc
     2161 gccgcccgca tcgggctcgg ggacagtgaa gagccggtcg tagacgcgca cctcggcgtc
     2221 aatcgcatgg gccgcgctca cccaatggat ggtgccttta accttgcgtc cggaggtcgg
     2281 gccgccgctc ttgctgccga ggtcggcggt gcagcgcagc tccaccacct ggccggcggc
     2341 gtctttgacc acctcgtcgc acttaatgat gaaggcgtat ttgagccgca cttcgccgcc
     2401 ggggcgcaga cggaagtact tgggcggcgg cgtttccatg aagtcatcgc gctcgatgta
     2461 cagctcgcgg ctgaacggga gcttgcgctt cccggcgttc gggtcctcgg ggttgttgat
     2521 ggcttcgagt tcctcgacct ggccttcggg atagttggtg atgacgactt tgaggggccg
     2581 cagcacggcc aagcgccgtt gggcgcggtg attgaggtcc tcgcgaatgc aatactcgag
     2641 cacggagaca tccgtgatgc cgttgtattt ggtaatgccg atgttgtagg caaagtcgcg
     2701 gagggcttcc tgtgtaaccc cgcggcggcg caggccgctg atggtcggca tgcggggatc
     2761 gtcccaaccc gacacaaggt tctcgttgac gagttgcatg agcttgcgct tgctcatcac
     2821 cgtgtaaccc aggctcagcc gggcgaactc gtactgatgc g
//
LOCUS       DATCGS010000050         2863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_500012, whole genome shotgun sequence.
ACCESSION   DATCGS010000050 DATCGS010000000
VERSION     DATCGS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500012"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2230
                     /locus_tag="VIL39_01000"
     CDS             <1..2230
                     /locus_tag="VIL39_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY5480034.1"
                     /translation="FLRICEDRDIDTGRPLDRLVRSWRDDDDPAAARRGRQQPLELHE
                     EPPAHYGGSGLRAPKGTLWHTLVRHFRALDRRPPSHIPFFNGNLFKPHFSEELIVSDD
                     WLAHFLDELGDEESPYLFSYIPVEILGTIYERFLGKVVRPHGRGVTIEEKPEVRKAGG
                     VYYTPRYIVEYIVGQTVGKLLAGRKPEDALKLRILDPACGSGSFLIHAFEVVSEHCQQ
                     WLTDHPDQRKKGWCWLEENAVHLTTKAKRRILRETIHGVDLDPQAVEVTQLSLYLKML
                     ENENRATLAHERDLFGGDEALLPPLEHNIKCGNSLIASDFSLMPDDLVRVHAFDWPVQ
                     FPAIMKAGGFDAVVGNPPYRMLQPHNTEEQELDYLKAHYLAAEFKIELFHLFLQRGVS
                     LLKKDGFHGHIVPTTLLNNVYAESLRRWLMERCAIQHISVAQGHVFEDADVHTSVVIF
                     KAESNPKARAAHDVLTTASLSAEFAAAPLGFSRTKQARFSALPGATWNILLNEDNAPL
                     LQRLTSECVPLEEVARINRGLITGDRERYFSPRKLTAKHRPVLAGADVHRYATNPPSE
                     FVRFERPRTSGGCWDADVHLAPHKIVIRQIGVTPTASVITEPIAVTGNIFTARGSSLT
                     EEKFLLAVINSKLTAYFWRIMFADFKTSFPQVTIFSLSQVPVWKVDVAKPSDRARHDR
                     MVGLVDKMLALTANLRAATSDGERQTLQNAVTATDQQIDALVYELYGLTEEEIALVEG
                     NV"
     gene            2297..2530
                     /locus_tag="VIL39_01005"
     CDS             2297..2530
                     /locus_tag="VIL39_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEY5480035.1"
                     /translation="MMINEHDCVVLTQNVPEEGVMAGDVGTVVHIHGDHAGYEVEFMT
                     LTGHTLAVVSLLPGQVRPIARRDVAHVRELATA"
BASE COUNT          594 a          905 c          828 g          536 t
ORIGIN      
        1 cttcctgcgc atctgcgagg accgcgacat tgacaccggc cgcccgctcg accgcctcgt
       61 ccggtcctgg cgcgatgacg acgaccccgc cgccgcccgc cgcggacgcc agcagccgct
      121 cgaacttcac gaagaaccgc ccgcgcacta tggcgggagc ggcctgcgcg cccccaaagg
      181 cacgctctgg cacaccctcg tccgccactt ccgcgcgctg gaccgccgcc ccccctcgca
      241 catcccattc tttaacggca acctcttcaa accccacttc agcgaggaac tcatcgtcag
      301 cgacgactgg ctggcgcact tcctggatga attgggcgac gaggagtccc cctacctctt
      361 cagctacatc ccggtcgaaa tcctcggcac catctacgaa cgattcctcg gcaaggtcgt
      421 ccgcccgcac gggcgcggcg tgaccatcga agaaaagccc gaggtccgca aagccggcgg
      481 agtctattac accccgcgct acatcgtcga atacatcgtg ggccaaaccg taggcaagct
      541 gctcgcgggc cggaagccgg aggacgccct caagctccgc atcctcgacc ccgcctgcgg
      601 ctccggcagc ttcctcatcc acgcgttcga ggtcgtctct gagcattgtc agcaatggct
      661 caccgaccac ccggaccagc ggaagaaggg ctggtgctgg ttggaggaaa acgccgtcca
      721 cctcaccacc aaggccaagc gccgcatcct gcgcgaaacc atccacggcg tggacctcga
      781 cccgcaggcc gtggaggtca cgcaactatc gctctacctc aagatgctgg agaacgagaa
      841 ccgcgccacg ttggcccacg aacgcgacct cttcggcggc gacgaagccc tcctgccgcc
      901 gctcgaacac aacatcaaat gcggcaactc gctcatcgcc tccgacttct ccctcatgcc
      961 ggacgatctc gtgcgcgtcc atgccttcga ttggcccgtg caattcccgg ccatcatgaa
     1021 ggcgggcggg tttgatgccg tggtcggcaa tccgccatat cggatgctgc aaccacacaa
     1081 cacagaagaa caggagttgg attatctaaa ggcccattac cttgcggccg agttcaagat
     1141 tgaactcttc catctattcc tgcagcgggg cgtgtcgttg ctgaagaaag atggcttcca
     1201 cggacacatc gttcccacaa cgctattgaa caatgtttat gcagaaagtc tccggcgctg
     1261 gctgatggaa aggtgcgcta tccagcatat ttctgtggcc caaggccatg tgtttgagga
     1321 cgcggatgtt catacctcag tggtgatttt caaggctgaa tcgaacccga aagcccgcgc
     1381 ggcacatgat gttctaacca ctgcctctct cagcgctgaa tttgccgctg cgcctctggg
     1441 cttttcgcga acgaagcagg cgaggttctc cgcattgccc ggggctactt ggaacattct
     1501 gttgaacgag gacaatgcgc ccttgctaca aagactgacc agcgaatgcg ttcctttgga
     1561 agaggtcgcc aggattaatc gcggcttgat tacaggtgac agggagaggt acttttcccc
     1621 tcggaaactc accgccaagc atcgtcccgt ccttgcaggt gctgatgtac accgctacgc
     1681 gacgaacccg ccatccgaat tcgtccggtt tgaaagacca agaacctcag gagggtgctg
     1741 ggacgcagac gtgcatcttg cgcctcacaa aatagtcatt cgccaaatcg gggttacccc
     1801 aactgccagt gttatcaccg agccaattgc ggtgaccggg aacattttca cggcgcgggg
     1861 gagttccctg acggaagaga aattcctgtt agccgtaatc aactcaaagc tcacagcgta
     1921 tttctggcga atcatgttcg ccgactttaa gacctcattt cctcaggtca cgatctttag
     1981 cctctctcaa gttccggtgt ggaaggtgga tgttgcgaaa cccagcgacc gcgcccgcca
     2041 cgaccggatg gtgggcttgg tggacaagat gctggcgttg actgcgaacc tgcgagcggc
     2101 cacatcggac ggggagcggc aaacgttaca gaacgcggtc accgcgactg accagcaaat
     2161 tgacgcgctg gtctatgagc tttacggcct gaccgaagag gaaatcgcgc tggtggaggg
     2221 caatgtatga ggctgcctga ggcagagctt gcgctggtgg aacgggagaa ggtgctcggc
     2281 tatcctctgg aggcgaatga tgattaacga acacgattgc gtggtcctga ctcaaaacgt
     2341 gccggaagag ggcgtcatgg ccggcgacgt gggaacggtg gtgcatatcc acggcgacca
     2401 tgccgggtat gaggtcgagt ttatgaccct gaccggtcac acgctggcgg tcgtctcgct
     2461 gctgcctggg caagtacgtc ccatcgcccg ccgggatgtg gctcatgtgc gcgagctggc
     2521 cacggcctga cgcacgtcac ggcgcaagac gccgtcaggg ctgccgatct gacggcgaga
     2581 cgcgagatgc cgccatctgc ggaagggtca gacctagaaa aatgatggcc gtgatggagt
     2641 ctgcccgtcg ttacgcgtgg cgcctttgcc gtctcaatag cgctgctctt gcccaccaca
     2701 acacggccag tgacaaagtc gcgagatacc tggccccgca agtatggact gcggcggcaa
     2761 gcgaagcgcg acgccgcttt ggcttgaagt gacgcagggg gttgaaccag gcgctcttta
     2821 ctcgcaggga aagcggcgtc gcgcttcgct tgtcgccgca gtc
//
LOCUS       DATCGS010000051        10982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_153632, whole genome shotgun sequence.
ACCESSION   DATCGS010000051 DATCGS010000000
VERSION     DATCGS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153632"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIL39_01010"
     CDS             <1..273
                     /locus_tag="VIL39_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY5480036.1"
                     /translation="RQHARFRDKPKITCNARMGSRELKSHCALDEATMELLKFAMTDL
                     NLSARAYDRVLKVARTIADLAGAERIASDHVSEAIQYRSLDRQIWT"
     gene            455..622
                     /locus_tag="VIL39_01015"
     CDS             455..622
                     /locus_tag="VIL39_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480037.1"
                     /translation="MKSKTQDRILPHPRSANESARRYPAHACQVTGMDVLRAGHDVKV
                     LRYETHVTANV"
     gene            680..1033
                     /locus_tag="VIL39_01020"
     CDS             680..1033
                     /locus_tag="VIL39_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5480038.1"
                     /translation="MALLEITCTSAAGYLHILLRGHITSATAPQLEQTLAAAIAGQAA
                     PQCVLDLGDLVYTSSAGLRIFLTFAKRMKNAGGQLVFCAVQPAVHKVLEVSGFTQILT
                     LVPDVTAARDLFLPR"
     gene            1040..1930
                     /locus_tag="VIL39_01025"
     CDS             1040..1930
                     /locus_tag="VIL39_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5480039.1"
                     /translation="MHSAPQASGSDRESATRTAVIAGGIDVLVTLGALLAAHSAVILA
                     DFCKTLLEFMAVLLAWLTIRRIQQGAHHQFDYGVGKLENLSSLIVSLLMFLCLAVIIA
                     NATYSLTHPAHISGIGVWISVVSQILYAVINTRLVLQSRRLARERNSPLLEAQAGLFF
                     SKAVANGFILLSLGLSTLLAAYSWSLYIDPCASLIIAVSILFAALGIFKNSTLDLLDR
                     TLEEEHQICILRALARHFDRYEELREIRSRRSGSQVFVEIILGFAPAQPAAEIETAAR
                     QLKLELEREIPGSRVTIAIA"
     gene            complement(2104..4662)
                     /locus_tag="VIL39_01030"
     CDS             complement(2104..4662)
                     /locus_tag="VIL39_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY5480040.1"
                     /translation="MRNQPPATTGLPGLDAVLSGIEPGDNIVWEVNAIADYQEFVSPY
                     AAAAQAMGRRLVYFRFADHPALLSPAPGIEQHDLDPRCGFEAFVHSVHDVIETAGPGT
                     LYVFDCLSHLADTWGSDQSLGNFFQLTCPRLLDLETVTYFGIYRDRHSAYAIEPIRGT
                     TQFMLDVFRLEGRLYIRPVKVQYRSRDVMNTLHVRSADQFRPVKESAVLAPILSHTQW
                     PRLQSDRRVGYWRQLFRDAETVAEECREGRCSPERLAETLRRVRWALRVHRSGIAGLV
                     ERFLKLEDFLALRDRMIGIGSVGGKTLGMLVARAILRERDPDLAARLEAHDSFFVGAE
                     VFITFLVQNNVWWLREHQKKPESFLQGRDEGRKRILEGHFPPGILEQFQGMLDYFGES
                     PYIVRSSSILEDARGNAFSGKYESVFVTNRGSREKRKQALIEAVRRVYASVLDEEALL
                     YRKRRGLLESAERMALLIMRVSGAPNGRYYYPQAAGVGLSFNPFAWHKDIEPQAGVVR
                     LVFGLGTRAVDRSDDDYTRLVALNAPSRRPEADVDDMCEHSQRRMDCLDLQEDKFVSL
                     PFQDVIEQGPDFPLDLYLTRTAQGFPHITFDRLLRETPVARDLHRMLDILESVYEHPV
                     DIEFTLNFLGDGSYRIHLLQCRTFQIRREIGGMSLPAQTTHTTLLSAHGAVIGVSREQ
                     PLSRIIYIVTDKYAALPEQARYAVARLIGKLNRLHAVDDRGLMLIGPGRWGTHSPSLG
                     IPVSFTEISRASVVCEVVAMHESLIPDVSLGTHFFNDLVEHDMLYVAYFPKKTGNSIN
                     DEWFRKAPNRLLELESSAASLAEVVRVIDCSDAEQRVWLRADTTEQEALVFLRP"
     gene            complement(4700..6040)
                     /gene="gdhA"
                     /locus_tag="VIL39_01035"
     CDS             complement(4700..6040)
                     /gene="gdhA"
                     /locus_tag="VIL39_01035"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEY5480041.1"
                     /translation="MNTYIAEVLDVVARRNAGEPEFLQAAREVLESLAPVLKRHRKYR
                     DAHILERIVEPERQIIFRVAWQDDAGQMQVNRGFRLQFNSAIGPYKGGLRFHPSVNLG
                     ILKFLGFEQVFKNSLTTLPMGGAKGGSDFDPKGKSAGEVMRFCQSFMTELFRHIGPDT
                     DVPAGDIGVGGREIGYLFGQYKRLKGEFSGVLTGKGLNWGGSLVRPEATGYGAVYFAE
                     EMLKTRNDSLAGKVCTVSGSGNVAQYTIEKLLQCGAKPVTLSDSNGTIHDPAGFNPEK
                     LAWVLDLKNVRRGRIKEYAEKFRATYIPGERPWSVPCDCAFPSATQNEISGEDAKTLL
                     KNGCKLVSEGANMPSAPEAIDTYLQAGILYGPGKAANAGGVATSGLEMTQNSMRLGWN
                     REEVDQRLHRIMIDIHRNCRATAEDYGVPGNYVIGANIAGFVKVADAMLDQGLV"
     gene            6417..7826
                     /locus_tag="VIL39_01040"
     CDS             6417..7826
                     /locus_tag="VIL39_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5480042.1"
                     /translation="MKYWPAIIALGLVAGCARFHPQPLSPAETAAGLESRSLDNPDLK
                     AFLEKNLKRELSAWPAAKWDFEMLTLAAFYYQPSLDVARARWAVAEAGKITAGGRPNP
                     TLNVTPGYNTTTLTPSPWMPLGSLDIPIETAGKRGYRIAQAAGLSESARLNIASVAWQ
                     VRSGVRRSLVALQAAQEAEVLLREQQGFQTQNLGLLERQHEAGAVSAFEVSQAGIAAD
                     NARLALRDAERQSAEVRVQLAEAVGVRANALEGVKFTFESWGELPGEASLADARRQAL
                     LNRSDILSGLADYAASQAALQLEIAKQYPDVHLQPGYQFDQGDNKWSLGITVELPVLN
                     QNQGPIAEAKARRAEAVARFNALQASVLSEIDRAMAGYRAALQKRADADELLARSQKQ
                     EQRSRAMLDAGEISKGDLVALRLQLSASSLARLDALVKSQQALVQLEDALQSPLGLPA
                     SVWQTSPRVSESHQADTHP"
     gene            7823..8857
                     /locus_tag="VIL39_01045"
     CDS             7823..8857
                     /locus_tag="VIL39_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480043.1"
                     /translation="MKKTVFIIVLLAAAAGLAWLVWFKPAKPEEAEKKPDTEVPVHVG
                     KIKRVTLRGYVTAYGTVEAEPAGERPAASARVAPSVPGVVTEVKCVEGQRMDKGALLF
                     QLDSRVADVAAAFAEKSLERQKKLVQVEGTSQKALQEAEQQLAAVRAQQALLRVQAPL
                     AGTVVRVNVRPGEAVDLTTTLAEVVDLDRLVVSAQVPSSELAAIKAAQPAEVLADKSG
                     PPVLGTVTYISSEVDAKTGTALVRASVPANSGLRPGQFVTLRIVSEEHKDRLAVPVES
                     VVKDAEGSTVIALVQGDKAAQKAVKTGLREGGLIEVEADGLQADMPVVTEGAYGLPKE
                     TKIRVLAEGH"
     gene            8988..>10982
                     /locus_tag="VIL39_01050"
     CDS             8988..>10982
                     /locus_tag="VIL39_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5480044.1"
                     /translation="MTRTSVVGQFATRHSLAIAFISLALCLAGIYAAFKLPSSVFPQT
                     NFPRVVILVDNGVMPADEMMATITRPIEEAMKDIPGAVTVRSTTGRGSAEISVFFNWQ
                     VNMIQSELYVNSRLSQIRSALPATASATVWRLTFSAFPIIGVSLISPQRSITELWETA
                     RYELKPRFLRIPGVARVDLVGGRTPEYHVVVDPVRLQTANLSLAQVTDALEKNNLITP
                     TGMHEENHTLYLAVVDGRVHSIQDLEDLTVTVVNTHPVRIKDFARVERGPEPVFNVVT
                     AEGQNAVLLNIRSQADGSTLDIAKALQQVLHDLKRDLPPDMKTAYYYDQSLFVRASVR
                     SVWDAIVFGLILSVLIIYLFLKNWGITFTAILVIPVTVLITLVAMWMVGMSFNLMTLG
                     GIAAAMGLVIDDAIVVVEAIYAKIAAGRPRLEGIQEAIGEIFRPLVGSTLTPVVVFIP
                     LAFLEGITGVFFRALALTMVVALLTSLALAITLTPSLAAWFIRDREKLEHGHAPKSDE
                     GGFLLRRVLRLYEVAMRATLRHRWLTLGACGLVLLFGVGIYQRLESEFLPVMDEGAFV
                     IDYIAPPGTSLAETHRQLLLAEQVLIATPDVESFSRRTGARLALSIAEPNTGDFLVKL
                     KPNRKRKTEEVISELRAKFNATLPGIEWEFPGVLGDLIGDL"
BASE COUNT         2026 a         3464 c         3415 g         2077 t
ORIGIN      
        1 cgccagcacg cgcgctttcg ggacaagccc aaaatcacct gcaacgcccg catgggcagc
       61 cgtgagttaa aaagccactg cgccctggat gaggcgacga tggagctgct gaaattcgcc
      121 atgaccgacc tgaacctcag cgcccgcgct tatgaccgcg tcctgaaagt tgcccgcaca
      181 attgccgatt tggccggtgc ggaaaggatt gccagcgacc acgtctcgga agcgattcaa
      241 taccgttcgc tcgacagaca aatctggacg taaggccctc cgcgcaaggt ggcatctcgc
      301 agggaccccg tcgggctcct ctggaaagtc acgcagttga cctgtggcgt cacagcgcca
      361 ggcaacatct ccgccaacct gatgagatta tccgcccctg gctgcgcttg acatcccggt
      421 tcccggttgg cagcattgtg ggagtgtcgg acctatgaag agcaagactc aagaccggat
      481 tcttcctcat ccgcgcagcg ccaatgaatc cgcgcggcgt tacccagcgc atgcctgtca
      541 ggttaccgga atggacgtgc tccgagccgg gcacgatgtc aaggtgctgc gctacgaaac
      601 ccacgtaacc gctaacgtat gatctttgtt tttcgccggc tcactcctgg ccccgcctaa
      661 ttggccaccc gcacttgcca tggccctgct cgaaatcact tgcacctccg ccgcgggata
      721 tctgcatatc cttctccgcg gccatatcac cagcgccacc gcgccccagc ttgagcagac
      781 gctcgccgcc gcaatcgcag gccaggcggc ccctcagtgc gtcctggacc tgggcgacct
      841 ggtctatact tccagcgcgg gattgcgcat ctttctcacc ttcgccaagc ggatgaagaa
      901 tgccggcggc cagttggttt tttgcgcggt gcaaccggcc gtgcataaag ttctcgaggt
      961 gtcgggattc acccagattc ttaccctcgt gccagatgtg accgccgcgc gcgacctttt
     1021 cctcccccgc tgagcgtcca tgcattccgc cccccaggca agcggctcgg accgcgaatc
     1081 cgccacgcga accgccgtca tcgctggcgg gatcgatgtc ctggtgaccc tcggcgccct
     1141 tttggccgcg cactcggccg tcatcctagc ggacttctgc aagacactgc tcgagttcat
     1201 ggctgtcttg ctcgcctggc tgacgatccg ccgcatccag cagggcgccc accaccagtt
     1261 cgactacggc gtgggcaagc tcgagaacct ctccagcctg atcgtcagcc tgctcatgtt
     1321 tctgtgcctg gcggtgatca tcgccaacgc cacgtattcc ctgacgcacc cggcgcacat
     1381 cagcggcatc ggcgtgtgga tcagcgtcgt gtcgcaaatc ctgtacgccg tcatcaacac
     1441 ccggctggtc ctgcagtcgc gccggcttgc ccgggaacgc aattcgccgc tgctggaagc
     1501 acaggcgggg ctgttcttct ccaaggccgt ggccaacggc ttcatcctcc tctctctcgg
     1561 tctcagcacg ttgctggcgg cctattcgtg gtcgctctac atcgacccct gcgcttccct
     1621 gatcatcgca gtctccatcc tgttcgccgc ccttggcatc ttcaagaact cgaccctcga
     1681 cctgcttgac cggaccctcg aggaagaaca ccagatttgc attcttcgcg ccctggccag
     1741 gcatttcgat cgctatgaag agctgcgcga gatccgctcg cggcgttccg gcagtcaggt
     1801 cttcgtcgag atcatcctgg gatttgcccc cgcccaaccc gccgccgaga tcgagaccgc
     1861 cgcccgtcaa ctcaagctcg agctcgaacg cgaaatcccc ggcagtcgcg tgaccatcgc
     1921 cattgcatag tgctcaaggg gggcgaaacc ggttcgcgca aagagttcag gactttgaaa
     1981 cacatttgcg agaattcacc acccgtttgc gaataatgga accgccactc cacaccgccg
     2041 gtgatgatgg agaacatcgc tgtattgttc cggcagttgt ggacgtaagc catcccgctc
     2101 ggctcagggc ctgaggaaga ccagcgcctc ttgctcagtc gtatcggccc gcagccagac
     2161 acgctgctcg gcatcgctac agtcaatcac gcggacgacc tctgccaggc tggctgcgct
     2221 gctttcgagc tccagcagcc ggttcggcgc cttgcgaaac cattcgtcgt tgatgctgtt
     2281 gccggtcttt ttcgggaagt aggccacata caacatgtcg tgctcgacca ggtcattgaa
     2341 gaagtgcgtg ccgagagaca catccgggat gaggctttcg tgcatcgcca cgacttcgca
     2401 cacgacggag gcgcggctga tttcggtaaa cgagaccgga atgcccaacg aagggctgtg
     2461 ggtgccccag cggccgggcc cgatcagcat gagtccccga tcatcaacgg catggagccg
     2521 gttgagcttc ccgatcagcc gggcgaccgc atagcgggcc tgctcgggca gcgccgcgta
     2581 cttgtccgtg acgatataaa tgatccggct gaggggttgt tcgcgcgaca cgccgatcac
     2641 ggccccatga gcggacagga gtgtggtatg cgtggtctga gcaggaagcg acattccgcc
     2701 gatctcgcgc cggatctgga acgtgcggca ctggagcaag tggatgcgat aggagccgtc
     2761 gccgaggaag ttgagcgtga attcaatgtc caccgggtgc tcatagaccg actcgaggat
     2821 atcaagcatg cggtgcaagt cgcgtgccac cggcgtttcc cggagcaacc ggtcgaacgt
     2881 aatgtgggga aatccctggg cggtccgggt gaggtacagg tcgagtggga agtcgggtcc
     2941 ctgctcaatg acgtcctgga aaggcagcga gacgaatttg tcttcctgca ggtcgaggca
     3001 gtccatgcgc cgctgcgaat gctcgcacat atcgtcaaca tccgcctcgg gccgccggga
     3061 gggcgcgttg agcgccacca gccgggtgta gtcgtcgtcg ctgcggtcca ccgcccgggt
     3121 gccgaggccg aagaccagcc gcacgacgcc ggcctgcggt tcgatatctt tgtgccacgc
     3181 aaacgggttg aaggagaggc cgacgccggc ggcttgcgga taataataac ggccattggg
     3241 cgcgccgctc acgcgcatga tgagcaacgc catgcgctcc gcgctctcca acagcccgcg
     3301 gcgcttccga tagagcagcg cctcttcatc gagcacgctc gcataaacgc ggcgcacagc
     3361 ctcgatcaac gcctgcttgc gcttctcgcg cgagccgcgg ttggtgacga agacgctctc
     3421 gtatttcccg gaaaacgcat tgccgcgggc gtcctcgagg atggaactgg agcgcacgat
     3481 gtagggggat tcgccgaaat aatccagcat gccctgaaat tgctcaagaa tccccggcgg
     3541 gaaatgtccc tccaggatgc gtttgcggcc ttcgtcccgc ccttggagga aagactccgg
     3601 tttcttttgg tgctcgcgaa gccaccagac gttattctgc accaggaacg tgatgaacac
     3661 ctcggccccc acgaagaagg aatcatgcgc ctccagacgg gcggccaggt cggggtcgcg
     3721 ctcgcgcaga atcgcgcggg cgacgagcat gccgagggtt tttccgccga ccgagccgat
     3781 cccgatcatg cggtcgcgga gggccagaaa gtcctcgagc ttcaagaaac gctccaccag
     3841 cccggcgatt cctgagcgat gcacgcgcag cgcccagcgc acccgccgca gcgtttccgc
     3901 cagccgctcc ggcgagcagc ggccttcgcg acattcctcg gccaccgttt cagcatcgcg
     3961 gaagagctgt cgccagtagc ccacccgccg atcgctctgc aggcgcggcc actgcgtgtg
     4021 cgagaggatg ggggccagca cggcgctctc cttcaccggc cggaactggt cggcgctgcg
     4081 cacatgcagc gtgttcatga catcccgcga ccggtactgg accttaaccg gccggatgta
     4141 gagccgccct tccagccgga acacatcgag catgaactgc gtggtgccgc ggatcggctc
     4201 gatcgcatag gctgagtgcc ggtcgcggta gatgccgaag taggtcaccg tctccaggtc
     4261 cagcagccgc gggcaggtga gctggaagaa gttgccgagg ctctggtcgg agccccacgt
     4321 atcggcgagg tgcgacaggc agtcgaacac atagagcgtg cccgggccgg cggtttcgat
     4381 cacgtcgtgc acgctgtgca caaatgcttc gaagccgcac ctgggatcca ggtcgtgttg
     4441 ctcgatgccg ggcgccggcg acagcagcgc agggtgatcg gcaaagcgga agtaaaccag
     4501 acggcggccc atcgcctggg ccgccgccgc ataaggcgac acaaactcct gatagtcagc
     4561 gatcgcattc acctcccaca cgatattgtc gcccggctcg atgccgctca gcaccgcatc
     4621 gagccctggc aggccagtcg tcgctggagg ttggttccgc atgctcatct actggccccg
     4681 gaacacccac cggcttgctt cagaccaatc cctggtcgag catcgcatcg gccactttga
     4741 cgaagccggc gatgttggcg cctatcacgt agttcccggg cacaccgtaa tcctcggccg
     4801 ttgcgcggca gttacggtgg atatcgatca tgatgcgatg cagccgttga tccacttcct
     4861 cgcggttcca gcccaggcgc atcgagtttt gtgtcatctc caggccggag gtggccacac
     4921 cgccagcgtt ggcggctttg ccggggccat agaggatgcc ggcttgcaga taggtatcaa
     4981 tggcctcggg tgcggacggc atattcgcgc cctcggagac cagcttgcag ccattcttga
     5041 gcagcgtctt cgcgtcctcg ccgctgatct cgttttgggt ggcgctcgga aaggcgcagt
     5101 cgcacggtac cgaccagggc cgttcacccg ggatgtaggt ggcgcggaac ttctccgcat
     5161 actccttaat gcgcccgcgg cgcacgttct tgaggtccag cacccacgcc agcttctccg
     5221 ggttgaaacc ggccggatca tggatggtgc cgttagagtc agataaggtc acgggcttgg
     5281 ctccgcattg gaggagcttc tcgatggtgt attgcgccac attgccggag ccggagacgg
     5341 tgcagacctt gcccgcgagc gaatcgttgc gggtttttag catttcctcg gcgaaataga
     5401 cggcgccgta gccggtggcc tccggcctga cgagcgaacc gccccagttg agccctttgc
     5461 ctgtaagcac cccggagaac tcgcccttaa gccgcttgta ctggccgaac aggtaaccga
     5521 tctcgcggcc acctacgccg atgtcacccg cgggaacgtc cgtatcgggg ccaatgtgcc
     5581 ggaaaagctc agtcatgaag ctctggcaga agcgcattac ctcgcccgct gacttgcctt
     5641 tcgggtcgaa gtcagagccg cctttggcgc cacccatcgg cagcgtggtg agcgagttct
     5701 tgaaaacctg ctcgaacccg agaaacttga ggatgccgag gttgacgctc gggtgaaaac
     5761 gcaggccgcc cttgtacggt ccgatggcgc tgttgaattg cagacgaaac cctcggttga
     5821 cctgcatctg accggcatcg tcctgccagg caacgcggaa tatgatttgt cgttccggtt
     5881 ccacaatccg ctcgaggata tgcgcatccc ggtacttgcg atgccgctta aggaccggcg
     5941 cgagagactc gagcacttca cgcgcggctt gaaggaactc aggctcgccc gcattccggc
     6001 gtgccaccac atcgaggact tctgcaatat aagtattcat ttattggatt tctctgaacc
     6061 ggcgccacgg ccggtgcgcc acattacgac ctcgggcttg ggggacgcaa gcctggatct
     6121 gaagacatcg aaagcgtgtt gctgcggatc tggtttggat cacagtcgcc cgcaaacgcc
     6181 cacgcatgga cgagcttgcc gcttccaacc cgtcttcgcc ccgacggatt tctaccagac
     6241 gccggcggcc ttggccttcc atagcggccc gtccggatga cgatactgtc atgcgggttt
     6301 catcctgccg tcacacgggt ttggtgagct gcgcggcgcc ggaaacaatt ccggcagacg
     6361 aagagcgtca tgtagttctt gatttgttta gccgtttcca attgagttaa cgccacttga
     6421 agtattggcc agccatcatt gcgttggggc tggtggccgg atgcgcccgg ttccacccgc
     6481 aaccgctgtc tccggcggag accgctgccg gcttggaaag ccgttcgctg gacaatcccg
     6541 acctcaaggc gttcctggaa aagaatctca agcgtgaact gagcgcttgg ccggcggcga
     6601 agtgggattt cgagatgctc acgctggccg cattttacta tcagccgagc ctcgatgtgg
     6661 cgcgggcgcg ttgggcggtg gcggaggctg ggaaaatcac ggctgggggg cggcccaatc
     6721 cgacactcaa cgtcacgcct ggctacaaca ctacaaccct gacgccttct ccgtggatgc
     6781 cacttgggtc cctcgacatt ccgatagaga cggcgggcaa gcgcggctat cgcattgccc
     6841 aggcggcggg gctttcggaa tcggcgcggt tgaacatcgc gtcggtcgcc tggcaagtgc
     6901 ggagcggagt aaggcggagc ctggtggcgc tccaggcagc gcaagaggcg gaggtgttgc
     6961 tgcgggagca gcaaggcttc cagacccaga atcttggtct gctggagcgc caacacgagg
     7021 cgggcgcagt ctcggctttt gaagtcagcc aggcgggcat cgcggcggac aacgcgcgtc
     7081 tcgcgttgcg ggacgccgag cggcaaagcg ccgaggtccg ggttcagttg gccgaggccg
     7141 ttggcgtccg ggccaacgcg cttgaagggg tgaaattcac gttcgagagc tggggcgagt
     7201 tgcccggcga agcttctctg gccgacgctc gtcggcaggc tttgcttaat cgctcggata
     7261 tcctgagcgg gctggccgat tatgccgcca gccaggctgc gctgcaactg gaaatcgcca
     7321 aacagtatcc cgacgtccac ttgcagcccg gctaccaatt cgatcagggg gataacaaat
     7381 ggtcgctcgg aattacggtc gagctgccgg tcctgaacca gaatcagggg ccgattgccg
     7441 aggccaaagc gcggcgcgcg gaagcggtgg cccgattcaa cgcgctccaa gccagtgttc
     7501 tttccgagat tgaccgtgcc atggcggggt atcgcgcggc gttgcagaag cgggcggacg
     7561 ccgatgagtt gctggcccga tcgcagaagc aggagcagag gtcgcgcgcg atgttggacg
     7621 cgggcgagat ttccaagggg gatttggttg cgctgcgcct gcaactgagc gcatcctcgc
     7681 tggcccgtct cgatgcgctg gtcaaatcgc agcaggcgct ggtgcagttg gaagacgcgc
     7741 tgcaaagtcc tttgggtctg ccggcctccg tctggcagac ctcgccacga gtttcggaat
     7801 cccaccaagc ggacactcac ccatgaaaaa gaccgtcttc atcatcgtcc tcctggctgc
     7861 cgcggcgggt ctcgcctggc tggtctggtt caagccggcc aaaccagagg aggccgagaa
     7921 gaagcctgac acggaggtcc cggtccacgt cggcaagatc aaacgcgtta cactgcgggg
     7981 ctatgtcaca gcctacggca cggtcgaggc ggaaccggct ggcgagcgtc cggcagccag
     8041 tgcccgggtg gcgccgtctg ttcccggagt ggtgacggaa gtcaaatgcg tggaaggcca
     8101 gcgcatggac aaaggggcgt tgttgttcca gctcgacagc cgggtggcag atgtggccgc
     8161 agccttcgcc gaaaagagtc tggagcggca gaagaaactg gtccaggtgg aaggcacatc
     8221 gcaaaaggcc ttgcaggaag ccgagcaaca actggccgcc gtccgcgcgc agcaagcgct
     8281 gctgcgagtg caggcgcccc tcgctggcac ggtggtgcgc gtaaacgtga ggccgggcga
     8341 agcggtggac ctgacgacca ctctggcgga ggtggttgat ctggaccggc tcgttgtcag
     8401 tgcccaggtg cccagcagcg aactggctgc gatcaaggcg gcgcaacctg ccgaagtgct
     8461 cgcggacaag tccgggccgc cggtcctggg cacggtgacc tacatcagtt cggaggtgga
     8521 cgcaaaaacc ggcacggcgc tcgtgcgcgc cagcgtgcca gctaactccg gtctgcggcc
     8581 cggccagttt gtgacgctgc gcattgtcag cgaggagcac aaggaccgtc tggccgtgcc
     8641 ggtggagagc gtagtgaaag acgctgaggg cagcaccgtc atcgcgctgg tccagggcga
     8701 taaggcggcc caaaaggcag tgaaaaccgg gctgcgcgag ggcggcttga tcgaagtgga
     8761 ggcggacggc ttgcaggcgg acatgccggt ggtgacggaa ggcgcttatg ggctgcccaa
     8821 ggaaaccaag atccgtgtgt tggcggaagg gcattagaca gaaagatggg agacagcaaa
     8881 atggaatcga aatccggtgc gggccaaaga gtgaagccct tgggaagaag ggaatctcct
     8941 tcccattttt ctgtctatga tctttctgtc ttcctctttc ttctgttatg acacgcacct
     9001 cagttgtggg ccaatttgcc acccgccatt cgctcgccat tgcgttcatc agcctggcgt
     9061 tgtgccttgc gggcatctac gcggcattca agcttccgtc ctccgtcttt ccccagacca
     9121 atttcccgcg cgtcgtgatc ctggtggaca acggggtgat gcccgccgac gagatgatgg
     9181 cgaccatcac gcggcccatc gaagaagcga tgaaggatat tcccggggcc gttacggtgc
     9241 gctccaccac cggccgcggc tcggcggaaa tcagcgtctt cttcaattgg caggtgaata
     9301 tgattcagtc ggagctgtac gtgaacagcc gcctctcgca aatccgctcg gcgctgccgg
     9361 cgaccgccag cgcgacggtt tggcggctga cgttttcggc cttccccatt atcggcgtga
     9421 gtctcatcag cccgcagcgc agcatcactg agctatggga gacggcgcgc tacgaactca
     9481 agccgcgctt cctgcgcatt cccggcgtcg cgcgcgttga tctcgtgggc ggacgcacgc
     9541 cggagtatca cgtcgtggtt gacccggtgc gcttgcaaac ggccaacctc agcctggcgc
     9601 aggtcaccga cgccctggag aagaataatc tcatcacgcc gaccggcatg catgaagaga
     9661 atcacacgct ctacctggcc gtggtggatg gccgcgttca cagtatccag gacctcgagg
     9721 acctcaccgt gaccgtcgtg aacacgcatc cggttcgcat taaggacttc gcccgcgtcg
     9781 agcgcgggcc ggagccggtc ttcaacgtgg tcaccgccga ggggcagaac gccgtgctcc
     9841 tcaacatccg cagccaggct gacggcagca cgctcgacat cgccaaggcc ctccagcagg
     9901 tgctccacga cttgaaaagg gatctcccgc cggacatgaa gaccgcctat tactacgacc
     9961 aatcgctgtt cgtccgggcg tcggttcgga gtgtatggga cgccattgtc ttcggcctga
    10021 tcctctccgt gctcatcatc tacttgttcc tgaagaattg gggcatcacg ttcaccgcca
    10081 tcctggtcat ccctgtgacc gtgctcatca cgctggtggc gatgtggatg gttggcatga
    10141 gcttcaacct gatgacgctg ggcggcattg ccgcggccat ggggctggtg attgatgatg
    10201 ccatcgtggt ggtggaggca atttacgcca agattgccgc gggccgccct cgactcgaag
    10261 gcattcagga ggccatcggg gagatcttcc gtccgctggt gggctcgacc ctcacccccg
    10321 tcgtggtgtt cattccgctg gcgttccttg aaggcataac cggggtgttc tttcgcgcgt
    10381 tggcgttgac gatggtggtg gcgctgctca cgtcgctggc gctcgccatc acacttacgc
    10441 cctcactcgc cgcgtggttc atccgtgacc gcgagaagct ggaacatggc catgcgccca
    10501 agagcgatga aggcggcttc ctgctccggc gtgtcctccg cctctacgag gtggctatgc
    10561 gggcgacgct ccgccatcgc tggctgacgc tcggcgcgtg tggcctggtg ctgctgtttg
    10621 gcgtcgggat ctatcaacgg ctggaaagcg aattcctgcc ggtgatggac gaaggtgcct
    10681 tcgttattga ctacatcgcg ccgccgggca ccagcctcgc ggaaactcat cgccagctct
    10741 tgctcgccga gcaagtcctc atcgccacgc ccgacgtgga aagcttctcc cgccgcaccg
    10801 gcgcccgtct cgcgctgtcc attgccgagc cgaacaccgg cgatttcctg gtcaagctta
    10861 agccaaaccg caagcgcaag actgaggaag tcatcagcga attgcgcgcc aagtttaacg
    10921 ccaccctgcc cggcatcgag tgggagttcc ctggcgtgct cggcgacctc atcggcgacc
    10981 tt
//
LOCUS       DATCGS010000052         5235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_54763, whole genome shotgun sequence.
ACCESSION   DATCGS010000052 DATCGS010000000
VERSION     DATCGS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54763"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VIL39_01055"
     CDS             <1..972
                     /locus_tag="VIL39_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HEY5480045.1"
                     /translation="CLAYSLDHGRSWKHYEKNPILVHPERDPKVFWYAPGNHWVMLLY
                     GDSQYHVLTSTNLLSWKDEHHPIKDSFECPDLFELPVDGHRDHMKWVLIQGNGNYSIG
                     SFDGTEFKEETGRQPCDIGPNFYATQTWGNVETGDGRRIQTAWMRSPPFPNMPFNQQI
                     SFPCQLTLHSTPNGLRVFREPIREIATLHHGQDTWTNRTLRANETLPLEPAGQLYHIQ
                     AQVSIPEGARLSFNIRGAHINLTSKTIAIEPGTRSTSVPDQIKTVEILVDRASIEAFV
                     NGGELSSTTFVLPQENGLSVKAEGGSVTVQSLTVYPLDSAWKDGIGN"
     gene            complement(1022..1612)
                     /locus_tag="VIL39_01060"
     CDS             complement(1022..1612)
                     /locus_tag="VIL39_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480046.1"
                     /translation="MQISRSFLAACGAAYCLALLPLCAADADVESKLREALRQKMNEL
                     DAQPAQAVPQPAVAAPSPQKKKAPAPGAKPAPTARPTPPAQWTPTTAVERTAPAQLLS
                     PKAKSEPAVKATATAVKPSAPAPATAAQPAPAPQSPTKPKVTKGQQPLPPIEGPPPSL
                     TADKEQRLQELSAKYKADQITPEEYHQQRAKILAEP"
     gene            complement(1623..2657)
                     /locus_tag="VIL39_01065"
     CDS             complement(1623..2657)
                     /locus_tag="VIL39_01065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY5480047.1"
                     /translation="MAQASEELLGGLLKEVSRSFYLTLRVLPGKVRAPIGLAYLLART
                     TDTIADTELVALEQRLEALRHLRERIQGQRATPLDFGALAQRQGSQAEGVLLEKCEAS
                     LALLAGLPSADRQLVCEVLNTITSGQELDLRRFAGAAAGTLVALRTEEELDDYTYRVA
                     GCVGEFWTKMCRAHLFPRAPLDDAFLLANGVRFGKGLQLVNILRDVAADLRQGRCYLP
                     AESLAGLGLKPEDLLQPANEPRLRPLYHGYLDRADAHLLAGWAYTKALPRRSVRVRLA
                     CSWPILIGRETLKLLRGGNVLDPAQRIKISRREVKGLMLRSVLLYPWPGAWEKMVPTG
                     KAVAPPAILS"
     gene            complement(2714..4114)
                     /gene="argH"
                     /locus_tag="VIL39_01070"
     CDS             complement(2714..4114)
                     /gene="argH"
                     /locus_tag="VIL39_01070"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY5480048.1"
                     /translation="MKRTSPVSRSGRFAGGPAADVAQFTESISFDRRLWRHDIAGSMA
                     HATMLRKVGCLTHGELRAIIQGLEAIAQEIQRGTFRWQAELEDVHMNIEADLTRRVPA
                     GAKLHTGRSRNDQVALDMRLWLREQIVGLREEIRLLQRALVNFGERERDVVIPGYTHL
                     QRAQPVYLAHHLLAYVEMLARDSQRLQECFARVNVCPLGSGALAGSTLPLDRELVADL
                     LEFVDARGRPQVTQNSMDGVSDRDFAVEFCSSSALLAVHLSRLAEDLILWSSSEFNFI
                     RIADAYTTGSSLMPQKKNPDVAELMRGKSARVIGNLVALLTLLKGLPMTYNRDLQEDK
                     ERLFDTADTVRATVRLCAAMLQHTRANREACLAAASDPALLATDLADYLVRKGLPFRQ
                     AHHAVGALVALSERLGKPLNRLTLAELQSVEKKFGADASRIFDLKEALARRQLTGAPG
                     TKEVRKQLARWKKLTR"
     gene            4215..5096
                     /locus_tag="VIL39_01075"
     CDS             4215..5096
                     /locus_tag="VIL39_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480049.1"
                     /translation="MSEFKFACPVCGQHITADSSTSGGQIDCPTCFRKIVVPQAPASQ
                     DTKLILSAAQVGKPRPSSAEAVSQLGSLQTAAPRSSLPAVLALLVLLCAAGAVLFVFR
                     DRIFKSARAPTGTNALSQPAAPIALNPTYPVPTNIAWTLDLTNAAFPEAVAAGSIHGS
                     GFRCEKAVLRGGLLSLIQGKAWPWDLSLALNLFARQGEELSGKTVQIASDRSRAPRVV
                     LRWKDAQQQPATETISGGYALNLVFGEATNGRMPGKIFICLPDSSKSFVAGTFDAEIR
                     KPAPQKPRSPKAPKPKG"
BASE COUNT          928 a         1764 c         1576 g          967 t
ORIGIN      
        1 tgcctcgcct atagcctgga ccatgggcgc agttggaagc actatgaaaa gaacccgatc
       61 ctggtgcacc cggagcgcga ccccaaagtc ttctggtatg ctcccggcaa ccactgggtc
      121 atgctgcttt acggggacag ccaataccat gttcttacgt ccacgaatct gttgagttgg
      181 aaggatgagc atcatccgat taaggacagc ttcgagtgcc ccgacctatt cgagctgccg
      241 gtggacggac atcgcgacca catgaagtgg gtgctgattc aaggcaacgg caattattcc
      301 atcggcagct ttgacggcac cgagtttaaa gaggagaccg gccggcaacc ctgtgacatt
      361 ggtcccaatt tctacgccac gcaaacctgg ggcaatgtcg agacaggcga tggcaggaga
      421 attcaaaccg cctggatgcg cagcccgccc tttccgaaca tgccgttcaa tcagcaaata
      481 tcctttccct gccaactgac gctgcacagc acgcccaatg gcctgcgggt gtttcgtgaa
      541 cccatccgtg aaatcgccac gcttcatcac gggcaggaca cttggacgaa tcgcactttg
      601 agagccaatg aaacgttgcc gctggagcct gcgggacaat tgtatcatat ccaggcacaa
      661 gtcagcattc cagaaggagc cagattgagc ttcaatatcc gcggcgccca catcaacctg
      721 acttctaaaa ctattgccat cgagccaggg actcgttcca cctcggtccc tgaccaaatt
      781 aagacagtgg agattctagt ggaccgcgcc tccattgagg cgttcgtcaa tggaggcgaa
      841 ctttcatcca cgacgttcgt cctgccccag gaaaacggcc tgtccgtgaa agccgagggt
      901 ggttcagtca ccgtccaatc gctcactgtt tacccgctcg actcggcgtg gaaggatgga
      961 atagggaact aaaaatccgg ctgggtcggg gcggcaaggc gggcgggaga gcccggagtg
     1021 cttacggctc agcgagaatc ttggcgcgct gctggtgata ttcctcgggc gtgatctgat
     1081 cagccttgta cttggccgac aactcctgca ggcgctgctc tttgtccgcg gtgagggacg
     1141 ggggcgggcc ttcgatcggc gggagcggct gctggccttt ggtaaccttt ggttttgtcg
     1201 gggactgtgg cgctggtgcc ggctgtgcag ccgttgcggg ggctggcgcc gagggcttta
     1261 ccgcagtcgc ggtcgccttg acggcgggtt ctgattttgc tttcggcgac agaagctgcg
     1321 ccggtgctgt ccgttcgacg gcggttgtgg gtgtccattg ggccggtggc gtgggccgcg
     1381 cggtaggcgc cggctttgcc ccgggggcgg gggccttctt cttctgcggc gacggagcag
     1441 cgacggcggg ttgcggcaca gcctgcgccg gctgcgcgtc cagttcattc atcttctggc
     1501 gcagcgcctc gcgcagcttg gactctacat cggcgtcagc cgcgcagagc ggcagtagag
     1561 ccaggcaata cgccgccccg caggcggcca ggaaggatct tgaaatctgc atagggacct
     1621 ttctacgata aaatggcggg cggcgcaaca gctttcccgg tcggcaccat cttctcccac
     1681 gcccccggcc agggatagag caagactgaa cgcagcatga gtcctttgac ctcacggcgg
     1741 ctgattttga ttcgctgcgc cgggtcgagc acattcccgc cgcgcagcag cttgagggtc
     1801 tcccggccga tcaggatcgg ccacgagcac gccaaccgca cgcgcacgct gcggcgcggg
     1861 agggccttcg tgtaagccca gcccgccagc aggtgggcgt cggcacggtc gagatacccg
     1921 tggtagagcg ggcgcaagcg cggctcgttc gccggttgca gcaggtcttc cggtttcaag
     1981 cccaagcccg ccaggctctc cgcgggcaag tagcagcggc cctggcgcag gtcggccgcc
     2041 acgtcgcgca ggatgtttac cagttgcagc cctttgccga accgcacccc atttgcgagc
     2101 aggaatgcgt cgtccagcgg tgcccggggg aagagatgcg cgcggcacat cttcgtccaa
     2161 aactccccca cgcagccggc cacccgatag gtgtaatcat ccagttcttc ctccgtgcgc
     2221 agcgctacga gggtcccggc tgccgcgccg gcgaagcggc gtaagtcgag ctcctgcccg
     2281 ctggtgatgg tgttgagcac ctcgcacacc agttgccggt cggcggacgg caagccggcc
     2341 agcagcgcca ggctcgcctc gcatttctcc agcaggacgc cttcagcctg cgaaccctgc
     2401 cgctgcgcca gcgcgccaaa atccagcggc gtggcgcgct gcccctgaat ccgctcgcgc
     2461 aagtgccgca gagcctccag ccgctgttcc agcgccacca gttcagtgtc ggcgatggtg
     2521 tccgtcgtgc gcgccagtaa ataggccagg ccaatgggcg cgcggacctt cccaggcagc
     2581 acgcgcaggg tcaggtaaaa cgagcgcgat acttccttta gcaacccccc cagcagttct
     2641 tcggatgctt gcgccatgct cgctacgcga atggaatgcc gctccgccgc aaaccgtcac
     2701 ggcggcgcgg cgttcagcgg gtcagcttct tccacctcgc cagttgcttg cggacttcct
     2761 tcgtccccgg cgctccggtg agctggcgcc gggccaaagc ttccttcaag tcgaatatcc
     2821 tcgacgcatc cgcgccgaac ttcttctcga cggattgcag ctcggcgagg gtgagccgat
     2881 tcagaggctt gcccagccgc tctgataatg ccaccagcgc ccccaccgcg tggtgcgcct
     2941 gccggaaggg caggcccttg cggaccaggt aatccgccag gtccgtcgcc agcagcgccg
     3001 gatcgcttgc cgccgccagg cacgcttccc ggtttgcccg ggtgtgctgg agcatcgccg
     3061 cgcagaggcg caccgttgcg cgcaccgtgt ccgccgtgtc gaatagccgc tccttgtcct
     3121 cctgcaagtc acggttgtaa gtcatcggca ggcccttgag cagcgtcagc agggcgacca
     3181 ggttgccgat cacccgggcg ctcttgccgc gcatcagctc ggccacgtcg ggattcttct
     3241 tctgcggcat cagcgaagag ccggtggtgt aggcgtcggc gatacggatg aagttgaact
     3301 cgctgctcga ccacaagatc aggtcttcgg ccagccggga gaggtgcacc gcgagcagag
     3361 ccgagctgct gcaaaactcc actgcaaaat cccggtcgct caccccgtcc atcgagttct
     3421 gcgtcacttg tggccggccc cgcgcatcca caaattcgag caagtcggcc accagctcgc
     3481 ggtccagcgg cagcgtcgaa ccggccagcg cgccgctgcc caacgggcac acattgaccc
     3541 gcgcgaagca ttcctgcaac cgctggctat cgcgcgccag catctccacg taggccagca
     3601 ggtgatgcgc caggtaaacc ggctgcgccc gttgcaaatg cgtgtagccc ggaatcacca
     3661 catcccgctc ccgctcgccg aaattcacca gcgcccgctg cagcagccgt atttcctccc
     3721 gcaaccctac aatctgttcc cgcagccaga gccgcatatc cagcgccacc tggtcgttgc
     3781 gcgagcggcc ggtgtgcagc tttgcccccg ccggcacccg gcgcgtcaag tccgcctcga
     3841 tgttcatgtg aacatcttcc agctccgcct gccaccgaaa tgtgccccgt tggatttcct
     3901 gcgcgatggc ttccagcccc tggatgattg cccgcaactc accgtgcgtc aggcagccga
     3961 ccttgcgcag catggtcgcg tgggccatcg acccggcgat gtcgtgccgc cagagccgcc
     4021 ggtcgaacga gatggattcg gtgaactgtg ccacgtcggc cgccgggccg ccggcaaacc
     4081 gtccgctgcg ggagacaggt gaggttcttt tcatgcgctt cgctcgcgtc gaatgtgcgg
     4141 cgagccggcg cgaaagtcac tgcaaaaaag cattgtcgtc tcgtgccgcc tgtggaatcg
     4201 ttgccgtgga agcaatgagc gaattcaaat ttgcgtgtcc ggtctgcggt cagcatatca
     4261 ccgccgactc cagcacaagc ggcgggcaaa tcgactgccc aacctgcttc cggaagatcg
     4321 tggtgcccca ggcacctgcg tcgcaggaca cgaagttgat tctatcggct gcccaggtgg
     4381 gcaaaccccg cccctcttcc gccgaggccg tttcccaact gggctccctg caaactgcgg
     4441 ccccccgcag ctcccttccg gcggtccttg cgttgctggt cctcctctgc gctgccggcg
     4501 cggttctctt tgtcttccgc gaccggattt ttaagtctgc tcgcgcgccg actggcacaa
     4561 acgcgctgtc gcagccagcc gccccgatcg cgctcaaccc cacctatccc gtcccgacga
     4621 acatcgcctg gacgctggac ctgacgaacg cagctttccc ggaagccgtc gcggcgggca
     4681 gcattcatgg tagtggtttc cggtgcgaga aggccgtctt gcggggcgga ctcctgtccc
     4741 tgatccaggg caaggcctgg ccatgggatc tgtcactcgc actgaacctt ttcgcgcggc
     4801 agggagagga gttgagcggc aagaccgtcc agatagcctc ggatcgttcc cgcgctcccc
     4861 gcgtcgtctt gcgctggaag gacgcccagc agcagcctgc caccgaaacc atcagcggcg
     4921 gttacgccct caacctcgtg ttcggcgaag ccaccaacgg gcgcatgccc ggcaagatct
     4981 ttatctgcct gcccgattcc tccaagagtt tcgtggcggg caccttcgat gccgagatta
     5041 gaaaacccgc gccccaaaaa ccgcgctcgc ctaaagcccc caagcccaaa ggctgacccg
     5101 ccgcttaatc cgctttgctc gatcaggcgc ccagccgctc actcaaacgc ctgaactgct
     5161 ccagcggttt cccacgtcga agcagccctg acgtcccgca gccttctgac tatgaaactc
     5221 accaccaggc ccctg
//
LOCUS       DATCGS010000053         5027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_450531, whole genome shotgun sequence.
ACCESSION   DATCGS010000053 DATCGS010000000
VERSION     DATCGS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450531"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VIL39_01080"
     CDS             <1..204
                     /locus_tag="VIL39_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480050.1"
                     /translation="LKVLRVDPATAHIPVVALSANAMPRDIEMGLDAGFFRYLTTPIK
                     IKELMDTLNTALEFAEEELCRRK"
     gene            943..2358
                     /locus_tag="VIL39_01085"
     CDS             943..2358
                     /locus_tag="VIL39_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480051.1"
                     /translation="MFNSSVLDVAIGLIFVYLLLGLMCTTVNEWLAQLFKTRAATLRE
                     GIRRLLHAPPDGTYLIRSADINVAALATRFNKADDKLTQAVGPFDAALQTSFDQFETA
                     LKERSDAQPPAGLAAALAEKLSAALSRPDLNQRIDDSKVTPETKAEIAKQLKGNDLLR
                     VNRALLSEAYPDEITSLSDSFYSHPLIKSLGRPGKHPSYVPSKTFALTLLDIFAKGQA
                     TTGTAEQKLAQIKASIDNLPDSDVKKSLQALLMHGSDSVEKVEEKLEGWFNDSMDRVS
                     GWYKNRVQIWTVVIASLVTILANADTIQIAQKLMINPALRDKIVQEAKTANAPSKDQT
                     PPTLTAQQKADLSSLTGWTDEFRIFHHLDACADQTLRGADVTEADCRAESDEQAKANA
                     NPKFVAAWNRNAFPGMRLLSGVAFPWLWAVVPGHLLGWILTAIAASLGAPFWFDTLSK
                     FMNVRAAGNSPTEKGADKSKS"
     gene            2452..3069
                     /locus_tag="VIL39_01090"
     CDS             2452..3069
                     /locus_tag="VIL39_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480052.1"
                     /translation="MLEGEMLLQSRYWLAFLKGDGARMAQLVSAAMGKPGAEDLLLAT
                     QADTEGWYGKLKNAQELTGRAMDSAQHHDAKEAAAAYQAAAALREVEAGNREQARAEA
                     HAAVKLAPNRDVRAIAALALARAGDTAGAEKLAGELEKNFPLDTLVQRYWLPTIRAGV
                     ALEHKDPKLECCFGGGAALGLRPRDFFPGRAARGRGNASPHKGVV"
     gene            3169..3474
                     /locus_tag="VIL39_01095"
     CDS             3169..3474
                     /locus_tag="VIL39_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480053.1"
                     /translation="MQPDDLSQLELMFNRFERLLGEVMRGALARNAFLPWELDILVDM
                     ETCHLDGRRRMDILTQYRKAVSRQMRTGPGPPMKLSEFLAVRARRRVISASGSDARA"
     gene            3614..4309
                     /locus_tag="VIL39_01100"
     CDS             3614..4309
                     /locus_tag="VIL39_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEY5480054.1"
                     /translation="MSVRHKEFHHTERVGWLRAAVLGANDGILSTASLVLGVAAAHAT
                     HGNVLVAGIAGLTAGSMSMAAGEYVSVHSQVDTEQADLARERAELKAHDKSEHQELAA
                     IYVARGLDASLAKQVAGQLMAHDALGAHARDELGFSATSNPRPIQAAAASAASFAVGA
                     ALPLVVTALASEAGLVPLVAGTSLAFLALLGGLAARAGGAGLTAGAIRVTFWGALAMA
                     VTAGVGALFGTAA"
     gene            4367..>5027
                     /locus_tag="VIL39_01105"
     CDS             4367..>5027
                     /locus_tag="VIL39_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5480055.1"
                     /translation="MPIDARPAGARQFFSRHGTLSKWHPNYEFRQGQVEMAEAVEAAF
                     SEKRHLIVEAGTGTGKTLAYLVPALLSGKRIVVSTGTKNLQEQLFFKDVPFLQKHFAR
                     PLKVSYMKGRNNYACRQKIYDADKEPVLTGLEEVADFQIVREWEKTTEFGDRAEIKKL
                     PENSTAWAKLDARGDMCSGQKCPNFERCFLTLMHQRAQESDVVIVNHHLFFADLALRD
                     ES"
BASE COUNT         1033 a         1499 c         1601 g          894 t
ORIGIN      
        1 ttgaaagtct tgcgcgtaga cccggccacg gcgcacatcc ccgtcgttgc cctcagcgcc
       61 aatgccatgc cccgcgacat cgagatgggc ctggacgcgg ggttcttccg ctatctcacc
      121 acacccatca agatcaaaga attgatggat acgctgaaca cggcactgga atttgcagaa
      181 gaggaacttt gtcggcgcaa atgaagcggg aggaatgtta tggtcagttc atctgtcttt
      241 ctgctcgagc ggatcctgcg tggcgccggc tatgtttcaa tcacgtctac gatggccggc
      301 gatgccgttc tcgacagaac gggctattct tctttgcgtc gactacaccc ttcactgcat
      361 cgactcgaca tgttccgggg gaatcgtcaa gctcatggcc ccgttagtaa accacaaccc
      421 gtgccgtctt tgacgagaag cgttatctgt cattatcggt agcgattgcc gactcttctc
      481 cgttgccacg gtcgcgttcg ctggccgggg ccgcgatccg gatacgcgcg ctgagcgact
      541 ccagtcatgt ggacaccggg cggtgagccc attttctaat cccgagcaat tccagcggcc
      601 tgggaaggcg ccagggcagc ccttgccgac cggatagggg ggggcgggca agcaaatagg
      661 ttgatggaag taggagggac ggagaggtgt atgagaaacc gcttgaaatg aggcacttcc
      721 gggtttttcc gtgcccggag gagcagagtt ggccccttcc tgggccgggg tagctcagga
      781 tggaaaggat ttaaaaaaaa aaggctctca cggaggcgat tggatgtata attgagcgcg
      841 gccggaaagc caaaacggaa accccggttg acttcttgcc gcaaaggaaa taatctttgc
      901 aaatactttg gactcagact ggattaggcg gaaggagacg aaatgttcaa ttcgagtgtc
      961 ctcgatgtcg caattggcct gattttcgtt tatctcctgc tggggctgat gtgcaccacg
     1021 gtgaacgagt ggctcgcgca gcttttcaag actcgcgccg cgacgctaag agagggaatc
     1081 cgccggctgc ttcacgctcc gccggacggc acgtacctga tccgctccgc ggatatcaat
     1141 gtggcggcgc tggcgacgag attcaataaa gccgatgaca agctcacgca ggccgttggg
     1201 ccgttcgacg cagccctaca aacgagcttt gatcaatttg aaacggccct gaaggagagg
     1261 tcggacgcgc aacctccggc aggcctggcg gcggcattgg cggagaaact cagtgcggct
     1321 ttaagccggc ccgacctcaa tcaaaggatc gacgatagca aggtcacccc ggaaaccaag
     1381 gccgaaatcg caaagcagct caagggaaac gatctgttac gcgtcaaccg ggcactgctg
     1441 agcgaagcat acccggatga aatcacgtcc ctctcggatt ccttctacag ccatccgctg
     1501 atcaagtcat tgggccgtcc gggcaagcat ccctcgtacg ttccgtcgaa aacgttcgcg
     1561 ctcaccctcc ttgatatttt cgccaagggt caggccacca cggggactgc ggagcaaaaa
     1621 ctggcgcaaa tcaaagcatc catcgacaat ttgccggaca gcgacgtgaa gaagtcgctc
     1681 caggctcttc tcatgcatgg cagcgactcg gtggagaagg tcgaggagaa gctcgagggc
     1741 tggttcaacg attcgatgga ccgcgtatca ggatggtaca aaaacagggt ccagatctgg
     1801 accgtagtga tcgcgagcct ggtcacgatc cttgcaaacg cggacaccat ccagatcgcg
     1861 cagaaactga tgatcaatcc ggcgctgcgc gacaaaatcg ttcaggaagc caaaaccgcg
     1921 aatgccccca gcaaggacca gacgccgcca accctgacgg cgcagcaaaa agccgacctc
     1981 agcagcttga ctggctggac cgacgagttc cggatctttc accatctaga cgcctgcgcg
     2041 gatcaaaccc tgcgcggcgc cgacgtgacg gaagcggatt gcagggccga gtccgacgag
     2101 caggcaaaag ccaacgcaaa tcccaagttc gtcgcggcct ggaacaggaa cgcgtttccg
     2161 ggcatgcgcc tgctgagcgg cgttgcgttc ccatggctgt gggctgtagt tccaggccac
     2221 ctgcttggtt ggattctaac cgcgattgcc gcttccctgg gagcaccttt ttggttcgac
     2281 acgctgagca agttcatgaa cgttcgcgcg gccgggaact cgcccaccga gaagggcgcc
     2341 gacaaatcca agtcataaag cggacggagg caaattcgtc accactttcg ggtaatacag
     2401 gatcgatcgt tgtaagccca ggccggcctc ccccgcgagc atgatcgcag cgtgctggag
     2461 ggcgagatgc tgctccagag ccgctactgg ttggcgtttc tgaagggcga tggggcgcgg
     2521 atggcgcagt tggtctcggc cgccatgggc aagccgggcg cggaagacct gctgctggcc
     2581 acgcaagcgg acacggaagg ttggtacggc aagctgaaga acgcgcagga gctgaccggg
     2641 cgggcgatgg attcagccca gcaccacgac gccaaagagg ctgctgcggc atatcaggca
     2701 gcggcagcgc tgcgcgaggt ggaagcggga aaccgggagc aggcgcgcgc cgaggcccat
     2761 gcggcagtga agctggcccc gaaccgcgat gtgcgggcca tagcggcgct ggccctggcg
     2821 cgggcagggg atacggcagg ggcggagaag ctggcgggag aactcgaaaa gaacttcccg
     2881 ctggacacgc tggtgcagag gtattggttg cccacgatcc gggcgggggt ggccctggag
     2941 cacaaagacc ctaaactaga gtgttgcttc ggagggggcg ccgctctcgg tctccgaccg
     3001 cgtgatttct tccctggccg tgcggcgcga gggcgaggca atgcctcgcc ccacaagggc
     3061 gtggtgtagg ggccggggca tgccagcccg cggagttgag gaacaaggca aacggtcgcg
     3121 gaaccagctc taatcctttt gccgtcaggc gccgatgaag acagtacagt gcagccggac
     3181 gacctttcgc aactggagct gatgttcaac cgattcgagc ggctgctcgg cgaagtgatg
     3241 cgcggggcgc ttgcgcgcaa cgcattcctg ccgtgggaac tggatatctt ggtggatatg
     3301 gagacctgcc atctcgacgg ccgccggcgt atggacatcc tgacgcagta tcgaaaggcg
     3361 gtttcgcgcc agatgcggac gggtccgggg ccgcccatga aactctccga gttcctggct
     3421 gtcagggcgc gccgccgcgt catctccgcc tcgggtagcg acgcccgcgc ttagccgcat
     3481 tacgcgctgc cccatggaag tggcgctcca agagcgaacc gtcgaaattg ccgaaaaccg
     3541 cgccaatttg ggcacttcgc cggattctcg cgcagagcgt tctaatctaa ggtggggtct
     3601 aaaggaggca agaatgtccg tacgccacaa ggaattccac catacggaac gcgtcggctg
     3661 gctgcgcgcg gcggtattgg gcgccaacga cggtatcctt tccacggcga gcctggtgct
     3721 cggtgtcgcg gcggcgcatg ccactcacgg caacgtattg gtcgcgggga tcgcgggcct
     3781 gaccgctgga tctatgtcga tggctgccgg cgagtatgtg tcggtccact cacaggtgga
     3841 caccgaacag gccgacctcg cccgggaacg cgcggaactc aaggcgcacg acaagagcga
     3901 gcaccaggaa ctggcggcga tctacgtcgc gcgcgggctc gatgcgtcgc tcgcgaaaca
     3961 ggtcgccggg caactgatgg cccatgatgc cctgggggcg catgcccgcg acgaactcgg
     4021 cttctcggcg acttccaatc cgcggccgat ccaggccgcg gcggcgtccg ccgccagctt
     4081 cgctgttgga gcagccctgc ctctggtggt cactgccttg gcctcggagg cgggactggt
     4141 tccccttgtc gctggaactt ccctggcgtt cctcgcgctt ctgggcgggt tggcggcgcg
     4201 cgcgggcggc gcgggcctga cggcgggcgc aattcgcgtc acattctggg gcgcactggc
     4261 catggcggtg actgcgggcg tcggggcgct gttcggaacc gccgcttgac cggggcccgg
     4321 ctcccaagac gatcttccgc cccactgtta gaatcaaact atccccatgc ccatcgatgc
     4381 gcggcccgcg ggcgcgcggc agttcttttc ccggcacggc acgctctcaa aatggcaccc
     4441 gaactatgag ttccgtcagg gccaggtgga gatggccgaa gccgtggaag cggcgttctc
     4501 cgaaaaacgg catctgattg tcgaggccgg gaccggcacc ggaaagacgc tcgcctatct
     4561 ggtcccggcg ctgctttcgg gcaagcgcat cgtggtttct accggcacga agaatttgca
     4621 ggaacaactc ttctttaagg acgtgccgtt cctgcagaag cattttgcgc ggcccctcaa
     4681 ggtcagctac atgaagggcc gcaataacta cgcctgccgg cagaaaatct acgacgccga
     4741 taaggagccg gtactcaccg ggctggaaga ggtcgccgat ttccaaatcg tccgcgagtg
     4801 ggagaagacc acggagttcg gcgaccgcgc cgaaatcaag aagctgcccg aaaacagcac
     4861 cgcctgggcc aagctcgatg cccgcggcga catgtgctcg gggcagaagt gccccaactt
     4921 cgagcgctgc ttcctgaccc tgatgcacca gcgcgcacag gaaagcgacg tcgtcatcgt
     4981 caaccatcac ctgttcttcg ccgatctcgc gctccgcgac gagtcct
//
LOCUS       DATCGS010000054         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_450539, whole genome shotgun sequence.
ACCESSION   DATCGS010000054 DATCGS010000000
VERSION     DATCGS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450539"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VIL39_01110"
     CDS             complement(<1..217)
                     /locus_tag="VIL39_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HEY5480056.1"
                     /translation="MHGVMGKPPGTANTATSRVMLLLLVAMTGVAPISLYMLVPALPV
                     LATTFGRDIGIAQMTVSLYMVGIACSQI"
     gene            493..1497
                     /locus_tag="VIL39_01115"
     CDS             493..1497
                     /locus_tag="VIL39_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEY5480057.1"
                     /translation="MGQCYGQLSLEERIEIYRLHVGGRSQNEIASALDRAPSTISREL
                     RRNSRPTKVWTGGYEPVRAQQLAERRRRWDGRFKLARQPGLQNHVGKSLAMGHSPEQI
                     AGRLALEHGRVIISHESIYRFIYHRAAQKDPWHRLLPRHKRTRGRHRRPGGSSASLIK
                     QRRSITERPAEVEGRRVPGHWEADFMLFARCGQGLLVLHERKTRFSIVQRPVDRKAVL
                     TARTIARQLGKLPKAVRKTISFDNGNEFAEHHRLHNTLGVRTFFCDPHSPWQKGGVEN
                     SIGRLRRTLPRKTDLNLITAAALKRHVQRLNNTPRKCLDFKTPAEAFSKLKSTVALQT
                     "
     gene            complement(1531..>2213)
                     /locus_tag="VIL39_01120"
     CDS             complement(1531..>2213)
                     /locus_tag="VIL39_01120"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY5480058.1"
                     /translation="GMGAPADIEKERAGIEADFRVKAVHSPADMTRPAEIADMVALGE
                     KTFGSVDVLVNNAGIQFVSPVEDFPIDKWDAIIAINLSSAFHGIRAVVPGMKKRGWGR
                     IINTASAHSLVASPFKSAYVSAKHGIAGLTKTVALELATFKITCNCVSPGYVWTPLVE
                     KQIPDTMKARNLTKEQVIHDVLLAAQPTKEFVTSEQVAALALFLCGDDAAQITGANLS
                     IDGGWTAE"
BASE COUNT          446 a          698 c          667 g          402 t
ORIGIN      
        1 tgatttgcga acaggcgatg ccgaccatgt agagcgacac ggtcatctgc gcgatcccga
       61 tatcgcggcc gaaagtggtg gccagcaccg gcagcgccgg caccagcata tagagcgaaa
      121 tcggcgcgac gccggtcatc gcgaccagca acagcagcat gacccgggag gtcgcggtgt
      181 tcgccgttcc cgggggcttg cccatcactc cgtgcatgcg gtccgatccg atctggtgaa
      241 tcggactgta gccttatctt tcacggggca taccggcgtt tcggaatgcg gccattccgt
      301 tttcgggagg tggctgctca aaaggcgagc gacaaggtcg gggcgtccag ccagatatac
      361 cgatgtcatc atccgcgaaa gcggatgatc cagtattcca gaggcggcag cgatggagtc
      421 gagaggctgc ggcgtactgg atgccccttg ggttcacgac acaagtgcaa cacctgctag
      481 ggtgttgtgg cgatgggaca atgttatggc cagctcagcc ttgaagagcg cattgagatc
      541 taccgcctgc atgtaggcgg tagatctcaa aacgaaattg cttctgcgct cgaccgggcg
      601 ccgtcgacga tcagtcggga attgcggcgg aactctcggc ccaccaaggt ctggacgggg
      661 ggctacgagc ctgtccgggc ccagcaattg gccgagcgcc ggcgccggtg ggatggccgc
      721 ttcaaactgg cgcgccagcc gggcctgcag aaccacgtgg gcaaaagcct tgcgatggga
      781 cattccccag agcagatcgc cggccggctg gcgctggaac atggtcgcgt catcatcagc
      841 catgagtcaa tctatcgttt tatctatcat cgtgcggccc agaaggatcc ttggcatcgc
      901 ctactgccgc gccacaaacg aacacgaggg cgccaccggc gcccaggcgg cagctccgcc
      961 agcctcatca aacaacggcg ctcgatcacc gaacgtcctg ccgaggtcga aggccgcagg
     1021 gtgccgggtc attgggaagc cgacttcatg ctgttcgccc gatgtggcca agggttgctg
     1081 gttctccacg aacgaaaaac ccgcttcagc atcgttcagc gtccggtcga tcgaaaagct
     1141 gtcctcaccg cccggactat cgctcgtcaa ctcggcaaac ttccgaaagc ggtacgcaaa
     1201 accatcagct tcgacaacgg aaacgagttc gccgagcatc acaggcttca caacaccctt
     1261 ggcgtccgaa ccttcttctg cgatccccat agtccttggc aaaaaggcgg ggtggaaaac
     1321 tccatcggac gcttgcggcg cacgctgccc cgcaaaactg acctcaatct catcacggca
     1381 gccgccctca agcggcatgt tcaacgcctt aacaataccc cacgcaaatg tctggacttc
     1441 aagacacccg ccgaggcatt ctccaaactc aaatcaaccg ttgcacttca aacgtgactc
     1501 catcccccgc cttcgcgggg catgacgacg ttactccgcg gtccagccgc cgtcgatcga
     1561 gaggttggcg ccggtgatct gggcggcgtc atcgccgcac aggaacagcg ccagcgcggc
     1621 gacctgttcg gaggtgacaa actccttggt cggctgcgct gccagcagca cgtcgtggat
     1681 gacctgctcc ttggtgaggt tgcgcgcctt catggtgtcg ggaatctgct tctcgaccag
     1741 cggcgtccag acatagcccg ggctgacgca gttgcaggtg atcttgaaag tcgcaagctc
     1801 cagcgccacg gtcttggtga ggccggcgat gccatgcttg gccgacacat aggccgactt
     1861 gaacggcgag gcgaccagcg aatgcgccga ggcggtattg atgatgcgtc cccagccgcg
     1921 cttcttcatg ccggggacga cggcgcggat gccatgaaag gccgacgaca ggttgatcgc
     1981 gatgatcgcg tcccatttgt cgattgggaa gtcctcgacc ggcgacacga actggatgcc
     2041 ggcattattg acgagaacgt ccaccgagcc gaacgtcttt tcccccagcg cgaccatatc
     2101 cgcgatttcc gcgggcctgg tcatgtcggc gggcgaatgg acagccttga ccctgaagtc
     2161 cgcctcgatg ccggcccgct ccttctcgat atcggcgggc gcgcccatgc cgt
//
LOCUS       DATCGS010000055         4619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_153870, whole genome shotgun sequence.
ACCESSION   DATCGS010000055 DATCGS010000000
VERSION     DATCGS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153870"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..2761)
                     /gene="hypF"
                     /locus_tag="VIL39_01125"
     CDS             complement(278..2761)
                     /gene="hypF"
                     /locus_tag="VIL39_01125"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEY5480059.1"
                     /translation="MSARLRIVVRGAVQGVGFRPFVYRLATGAGLNGWVNNSAQGVFI
                     EVEGLQADLEAFLLRLEVEKPPRSSIQSLEASWLDSVGYVGFEIRQSDTGGAKTALVL
                     PDIATCPDCLGEIFDSRNRRYGYPFTNCTNCGPRFSIIESLPYDRANTSMKGFTMCPQ
                     CQAEYNDPRDRRFHAQPNACPICGPQLELWDRQGRGVPSAGAQPSGCSHVGGPGTQPP
                     GSAAAVNPPLLQPKGCAPTRPDASRAAIAAAADAVRQGKIVAVKGLGGFHLMVAAPHD
                     DAVQRLRTLKHREGKPFALMFPSLPAIKSVCEVSPLEERLLRSPEAPIVLLRRIHTSR
                     ITHHVSALSPFLAPGNPNLGVMLPYTPLHHLLMSALGFPVVATSGNLSDEPICTDEHE
                     ALQRLGGIADLFLVHNRPIVRHVDDSIVRVMMGRELVLRRARGYAPLPIQLRSSTGDA
                     VLAVGAHLKNTITLAVGQQAFISQHIGDLETDQAHEAFRRVINDFQGLYESRPALIAA
                     DVHPDYLSTKFARDLGNADPQLSTLNPQLISVQHHVAHVLSCMAENDLEPPVLGVSWD
                     GTGYGLDGTVWGGEFFLVTETSCERVAHLRQFRLPGGDKAVKEPRRVALGLLYEMFGE
                     AAFSMTELAPVAAFSPGELGLLRTMLARNLNSPLTSSVGRLFDAVASLAGLRQQVHFE
                     GQAAMELEFALEGPTTAESYELPIVSGHESRITNHESLSLNWSPMVSAILADLKRGLP
                     IGQISARFHNALAEAIVAVARQIGQPRVVLSGGCFQNRYLTERSVRRLQEAGFRPYWH
                     QRVPPNDGGISLGQVVAAQHPGWAVRSGG"
     gene            3123..3794
                     /locus_tag="VIL39_01130"
     CDS             3123..3794
                     /locus_tag="VIL39_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480060.1"
                     /translation="MNINRQTLVALAAIAGALSLQSLARAADTQAPAQPAGGDRVAAL
                     RERMQDTANELNLTDEQKEKLQTIIREQTGKLRDLRQDASLSPEDKKAKARAIREEIM
                     AEVKKVLTPEQFEKWKAKQGQLAGGGGAPLARVQEAIKELNLTDQQKEQLKPIYEEQM
                     QKLRELYLDSTLSIPEKLEKLKGMHKEIAPKIKKFMDADQYAKWEKNVNQWIEQLRQR
                     FQDAK"
     gene            complement(3904..>4619)
                     /locus_tag="VIL39_01135"
     CDS             complement(3904..>4619)
                     /locus_tag="VIL39_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEY5480061.1"
                     /translation="PLTRGLEEEVTYATPSKLAEMLQRDALDAALVSVVEVLFNDRYD
                     ILDGIAIASLGEVKSVLLAHRKPLNEATEIFCDTASLTSVQLLRVLLAERGLKPQFKP
                     LATYDSATLPDYVMLIGDPALDFALGPHEHEIWDLGTAWFEMTKLPFVYAVWALRRGV
                     ENAALRRLLREARDFGLDTLDSIIRTRTDYDSDFRKDYLSWHIHYHLGSDEKRGLAKF
                     IELLRRHGCGPVFEPRFVV"
BASE COUNT          964 a         1288 c         1593 g          774 t
ORIGIN      
        1 ttttcctggg atagccaaac acattgcccc caagcttaca ctcacggagg caccggggca
       61 atgatggggc ggggggaaat ccgaaggccg aaatccgaga cccgaaagaa ggccgaaatc
      121 cgaaatccga gaatggcttg atacgatggg ctgctactcc gctggagtgg ctgtaaattc
      181 gggcttcggg cttcgggctt ctttcgagtt tcgggtttcg gccttcggat ctggaccgcc
      241 cggccgcggt ctcttcagtg cgtccaggga accgctttca gccgccagaa cgcaccgccc
      301 acccagggtg ctgcgcggca acgacctggc ccagggagat gccgccgtcg ttgggtggaa
      361 cgcgctggtg ccaatagggg cgaaagccgg cttcctgcaa gcgccgaacg gagcgctcgg
      421 tcaggtagcg gttctggaag cagccgcccg aaaggacgac gcgcggctgg ccgatctgcc
      481 tggcgacggc aacgatggct tccgccaggg cattatggaa ccgggcggag atttggccga
      541 tgggaaggcc gcgtttcagg tcggcgagga tggcgctgac catgggggac cagttgaggg
      601 agagtgattc gtgattcgtg attcgtgatt cgtgaccaga gacgatggga agttcgtagg
      661 actcggcggt ggtggggcct tcgagcgcaa actccagttc catggcagcc tggccttcga
      721 aatggacctg ctggcggagg ccggcgagcg aggcgacggc atcgaagagg cgcccgacac
      781 tggaggtcag cggtgaattg aggttgcgcg caagcatggt tctgagcagg cccaactcgc
      841 cgggcgagaa ggcagccacc ggcgccagct cggtcatcga gaaagcggcc tcgccgaaca
      901 tttcgtagag caggcccagg gcgacccggc gcggctcctt caccgccttg tcgccgccgg
      961 gcaggcggaa ttgacggaga tgggccacgc gctcgcagga agtctccgtc accaggaaga
     1021 actcgccgcc ccaaaccgtg ccgtcgaggc catagccggt gccgtcccag gacacgccga
     1081 ggacaggggg ttccagatca ttctcggcca tgcaggacag gacgtgggcg acgtgatgtt
     1141 ggacgctgat tagttgaggg ttgagggttg agagttgagg gtctgcattt ccgaggtcgc
     1201 gggcgaactt ggtggaaagg taatcggggt gcacgtcggc agcgatgagg gcggggcggg
     1261 attcgtagag gccctgaaag tcgttaatga cccggcgaaa ggcttcgtgg gcctgatccg
     1321 tttccaggtc gccgatgtgc tggctgataa aggcttgctg gcccacggcg agcgtgatgg
     1381 tgttcttcaa gtgggcgccg acggcaagga cagcatcgcc agttgaagaa cggagctgga
     1441 ttgggagagg ggcgtagccg cgagcccggc gcaggaccag ctcgcgtccc atcatgacgc
     1501 gcacgatgga gtcgtccacg tgccggacga tgggccggtt gtggaccagg aacagatcag
     1561 caatcccgcc gagacgttgg agcgcctcgt gctcgtcggt gcagatcggc tcgtcgctca
     1621 ggttgccgct cgtggcgacg accgggaacc caagcgctga catgagcagg tgatgcagcg
     1681 gggtgtaggg gagcatgaca ccgaggttgg ggttgcctgg agcgagaaag ggagacaacg
     1741 cggaaacgtg atgcgtgatg cgtgatgtgt gaatgcggcg gaggagaacg atgggggctt
     1801 cgggggagcg aaggaggcgc tcttccaggg gcgagacttc gcaaactgat ttgatggccg
     1861 gcagcgaggg gaacatcagg gcgaagggtt ttccctcgcg gtgcttcaac gtccgaaggc
     1921 gctggaccgc gtcatcatgg ggcgcggcga ccatgaggtg gaagccgccg agacctttca
     1981 cggctacgat cttgccctgt cggacggcgt ccgcagccgc agcaattgcc gcacggctcg
     2041 cgtccggacg agttggagcg cagcccttag gctgcagaag cggcgggttc acggcagcgg
     2101 cggagcctgg tggttgagtt ccaggacctc cgacatggct gcagcctgaa ggctgcgctc
     2161 ctgccgatgg cacccccctg ccctgccggt cccacaattc aagctgcggc ccgcagatag
     2221 ggcaggcatt gggctgggcg tggaagcggc ggtcgcgcgg gtcgttgtac tcggcctggc
     2281 actggggaca catcgtgaag cctttcatcg aggtgttcgc gcggtcatag gggagcgatt
     2341 cgatgatgct gaagcgcggg ccgcaattgg tgcagttcgt gaatggatac ccgtagcggc
     2401 gattgcgcga gtcgaagatc tcacccaggc agtcggggca agtagcgatg tcgggcagca
     2461 ccagggccgt cttggccccg ccggtgtcac tctggcgaat ctcaaaacca acgtagccga
     2521 ccgaatcaag ccaggacgct tcgaggctct gaatagagct gcgcggcggc ttttccacct
     2581 ccaggcggag caggaatgct tccaaatcag cctgcaagcc ttcgacctcg atgaacacgc
     2641 cttgggcgga attgttaacc caaccgttga gcccggcccc ggtagcaagc cggtaaacga
     2701 aggggcggaa gccgacgccc tggactgcgc cccggactac gattctcagt cgagcactca
     2761 atccaacctc acccacgatt ccgttacacg acaagcgaag gcaagttgga ccacgaccaa
     2821 ccccaggcac ggaaggcgca ggtttgtctc actgacgtcg aaattgaact cgctctggct
     2881 gctcacacgt tgcgtggtgt cggcattcac tctggcaagt tggggctgcg aaaacaaagc
     2941 aattcctcat ggcgcattgg aacggcccag cgttctgcac ccttgatgcc tgagggaagg
     3001 aaattgcttg cctgattcga aagatcgtaa ctaattgggg ccggtttccg tcttgaacct
     3061 ggcaacggcg tggcggtcga atatcaacaa accgccgtcc gggccttgaa aatacgatct
     3121 ttatgaacat aaacagacag acattggtgg cgctggccgc gattgccgga gctctgagcc
     3181 tgcaatcatt ggcgcgagcc gcggataccc aggcaccagc ccaacccgcg ggcggcgacc
     3241 gcgtggccgc cttgcgtgaa cggatgcagg acaccgccaa cgagctgaac ttgacggacg
     3301 aacagaagga gaagctccag accatcatcc gtgagcaaac ggggaagctg cgcgacttga
     3361 ggcaggacgc gagtttgtcg ccggaggaca agaaagcgaa ggcccgggcg atacgcgaag
     3421 agatcatggc ggaggtcaag aaggtgctga cgcctgagca attcgagaag tggaaggcca
     3481 agcaggggca actggccgga gggggcgggg cgccgcttgc acgagtgcag gaggccatca
     3541 aagaactgaa ccttacggac caacagaagg agcaactcaa gcccatttac gaagagcaga
     3601 tgcaaaagct gcgtgagttg tacctggaca gcaccctgtc catcccggag aagctggaaa
     3661 agctaaaggg tatgcataag gagattgctc cgaagatcaa gaagttcatg gacgcggacc
     3721 agtatgcgaa gtgggagaag aacgtaaacc agtggattga gcaattgcgg cagcggttcc
     3781 aggacgcgaa gtaggattag ctttggcgga ggggcgctcg gtccactgcg cgccccgccg
     3841 gtgcctgtgc gcccctgctg gccagggata atcgggaggt cgcgggggag accgccccag
     3901 gggttacacc acaaagcgag gctcgaagac gggcccgcaa ccgtgccggc gcagcagctc
     3961 gatgaatttt gcgaggccgc gcttctcgtc ggagccgagg tggtagtgga tgtgccagct
     4021 caggtaatct ttccggaagt cggaatcgta gtcggtgcgc gtgcgaatga tcgaatcgag
     4081 cgtatccagc ccgaagtcgc gcgcctcgcg gaggaggcgc cgtaacgcag cattctcgac
     4141 gccgcgacgc aaagcccaga cggcataaac aaacggcagc ttggtcatct cgaaccaggc
     4201 ggttccgagg tcccagatct cgtgctcatg cgggcccagg gcaaaatcca gcgccgggtc
     4261 accgatgagc atgacgtagt ccgggagcgt ggccgaatcg taggttgcga ggggcttgaa
     4321 ctgcggttta agcccgcgct cggcgagcag cacccgcagc aattgaacgc tggtgaggga
     4381 agccgtatcg cagaagatct cggttgcctc gttcagcggc ttgcggtgcg ccagcagaac
     4441 gctctttacc tcgcccaggg aggcaatggc aattccgtcc aggatatcgt agcggtcgtt
     4501 gaagaggacc tcgaccacac tgaccagcgc ggcgtccagc gcatcgcgct gcagcatctc
     4561 cgccagcttg gagggtgtgg cataggtcac ttcctcctcc agaccgcgcg tgagcggga
//
LOCUS       DATCGS010000056         4337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_550231, whole genome shotgun sequence.
ACCESSION   DATCGS010000056 DATCGS010000000
VERSION     DATCGS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550231"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VIL39_01140"
     CDS             <1..1439
                     /locus_tag="VIL39_01140"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5480062.1"
                     /translation="LRFTQFYNTGRCCPTRASVLTGLYPHQAGVGHMTEDNSVPGYRG
                     RLNDRCVTIAEVLRGAGYFTAMSGKWHVGQNHGVVPWERGFERSLNSPAGGFYYPEGK
                     QGRIFLNGQALADDAPELPKNWYTTDLWTDYGIKFVDEARAAQKPFFLYLAYNAPHFP
                     LQAPPDEIAKFRGKYKSGWDKLREQRHARQKELGVVDKAWPLSPRPPLVRAWDKLNDQ
                     EKDRFDHIMAIYAACVAHMDTAVGRVVAALKQRGVLDNTLILFMSDNGGNAESGPEGR
                     LEGDSPGAAGSTVFCGQSWATLENTPFRRYKHYNHEGGIATPLVVHWPAGFAARGELR
                     QQPGHLVDIMATCVDVAGAKYPVDFQGRTILPMEGRSLVPAFADKAVERDALFWEHEG
                     NAAIRVGDWKLVRFGRNGSWELYNLKADRTELHDLAADEPERAKELAAKWDAWAGRTN
                     VKPYPAPAQGGAKKGKKKGKAAKAAANL"
     gene            1559..2926
                     /locus_tag="VIL39_01145"
     CDS             1559..2926
                     /locus_tag="VIL39_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5480063.1"
                     /translation="MAIPVRPLHAAATVPTKLNILFILTDDMGYGDIGCYGGHFAPTP
                     NIDRLAREGIRFTQYYSASPICSPSRTGLTTGMFPARWNITSFLQTRAGNRACEQADF
                     LNPTAPSLARTLKAAGYATGHFGKWHMGGGRDVTNAPPFAAYGFDEHAGTYESPEPHP
                     DITATNWIWSTHDKVKRWDRTAFFVDKTLDFLQRHKGQPCYVNLWPDDVHTPWVPGQE
                     RLGLYPSGPVEERKFRAVLDEYDRQMGRLLEGLKQLGIEGRTLVIFTSDNGPLPSFRG
                     SRSGGLRGSKLSLYEGGIRMPFIVRWPGHAPAGRLDDQTVLSATDLFPSLCALAGVKL
                     PKNVAFDGEDLSAALLGTPIARAKALFWEYGRNTNSFAFPKGADRSPNLALREGDWKF
                     LINADSTDPQLYDLKSDPREAANLTSANPEVVDRLKEKALAWRKALPRMRLNAPPSSA
                     DREAR"
     gene            2932..3555
                     /gene="amrA"
                     /locus_tag="VIL39_01150"
     CDS             2932..3555
                     /gene="amrA"
                     /locus_tag="VIL39_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY5480064.1"
                     /translation="MTNEKDRVTGGAPGSENLAAAERKFLLELARETLTRVTAGGSLP
                     EVATKAVSPKLREKKACFVTLTKNGALRGCIGHLTAMEPLHEAVAENARNAAMRDPRF
                     PPIEPGELDQIKIEISVLTEPQPLAFSSPDDLLSKLHPNSDGVLLHLGPRTATFLPQV
                     WAQVPDKVEFLGHLSQKAGCERSAWRGKDVSVSIYHVECFEEEHARP"
     gene            complement(3628..>4337)
                     /locus_tag="VIL39_01155"
     CDS             complement(3628..>4337)
                     /locus_tag="VIL39_01155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5480065.1"
                     /translation="GLIGDYLSRHDRSRWLVATKFGHHFNKFQDRTDGFSAADVRKQL
                     EASLRALRVETIDLYQFHSGSDSQLLNEDLWGMLTEQKRAGKVRHLGISILGKGSEAQ
                     AREAQRLGVETLQVIYNRLDRRPEQQYFPQAQQHNLGILARVPLASGLLSGKYRPGAA
                     FPANDWRSTFKAEKMGQDLAEVERIRQTELPAGVPMAQWALAWCLKNPVVSAVIPGCK
                     DPAQVASNAAAAELADG"
BASE COUNT          838 a         1369 c         1358 g          772 t
ORIGIN      
        1 gtctccgctt cacccagttc tataataccg gccgctgctg ccccacgcgc gcttcggtgc
       61 tcacggggct gtatccgcat caggcaggcg tgggccacat gacagaggac aacagcgtgc
      121 ccggctatcg cggccgattg aacgaccgct gtgtcaccat cgctgaggtg ctgcgcggcg
      181 cgggctactt cacggctatg agcggcaaat ggcatgtggg ccagaaccac ggggtcgtgc
      241 catgggagcg cggttttgaa cgcagcctca actcaccggc gggcgggttc tactaccctg
      301 aaggcaagca gggccggatt ttcctgaatg gccaagctct tgccgatgac gccccggagc
      361 tgccgaagaa ttggtacacg accgacctct ggaccgacta cggcatcaag tttgttgacg
      421 aggcacgagc ggcccagaag ccattctttc tatacctggc ctacaacgcc ccgcacttcc
      481 cgctgcaggc cccgccggac gaaattgcga agttccgcgg caaatacaag agcggctggg
      541 ataaactccg cgaacagcgc catgccaggc agaaggaact gggcgtcgtg gacaaagcct
      601 ggccgctctc gccgcgtccg cccctcgtgc gagcatggga caaattgaac gaccaggaaa
      661 aggaccggtt cgatcacatc atggccattt acgccgcgtg cgtcgcgcac atggataccg
      721 cggtgggccg cgtcgtcgca gcgctcaagc agcggggcgt gctggacaac acgctcatcc
      781 tgttcatgtc cgacaacggc ggcaatgccg agagcggtcc cgaggggcgg ttggagggcg
      841 actcccccgg cgccgcaggc tcgacggtgt tctgcgggca gtcgtgggcg acgctggaga
      901 atacgccctt ccgccgctac aagcattaca atcacgaagg gggcattgcg acgccgctgg
      961 tggtccattg gccggcggga tttgccgcgc ggggtgagtt gcggcaacaa cctggtcacc
     1021 tggtggacat aatggctacc tgcgtggacg ttgcgggtgc aaaatatccc gtcgattttc
     1081 aaggccgaac gatcctcccc atggaaggcc gcagcctggt gccggcgttt gctgacaagg
     1141 ccgttgagcg ggatgcgcta ttctgggagc atgagggtaa cgccgcaatt cgcgtgggcg
     1201 attggaagct ggtgcgcttt ggccgcaacg gctcctggga actctacaac ctcaaggcgg
     1261 atcgcactga gttgcacgac ttggcggctg acgagccaga gcgagcaaag gaactcgccg
     1321 ccaaatggga tgcctgggcc gggcgtacca acgtcaaacc gtatcctgcc ccagctcagg
     1381 gaggtgccaa gaagggcaag aagaaaggca aggccgcaaa agccgccgcc aacctatgaa
     1441 ccgtcggctc aagatggtaa ccagtatgcg gatgcagccc ccgggcagcg tcttccactg
     1501 ctgggcagtg ttgaccggga gcaatctctt actcgcggtt gtggctgtgc tcgtcgggat
     1561 ggcaatacca gtgcggcccc tgcacgctgc cgcgacggtg ccgacgaagc tgaacatcct
     1621 tttcattctg accgacgata tgggctatgg cgacattggt tgctatggtg gccacttcgc
     1681 gcccacgccc aacattgacc gcctcgcgcg cgagggcatt cgcttcaccc agtattacag
     1741 cgcctccccg atttgctcac cctcgcgcac gggcctgacc acgggcatgt tcccggcccg
     1801 ctggaatatc accagctttc tccagacccg cgccggcaac cgtgcctgtg agcaggccga
     1861 cttccttaat cccacggcgc catcgctggc ccgcaccctc aaagcggctg ggtatgccac
     1921 cgggcacttt ggcaagtggc acatgggcgg cggaagggac gtgactaacg cgccaccctt
     1981 cgctgcctac ggatttgacg agcacgccgg cacgtacgaa agccccgagc cccaccccga
     2041 tatcaccgcg accaactgga tttggtccac gcacgacaag gttaagcgct gggaccgcac
     2101 cgcgttcttt gtggacaaga cgctggactt cctccaacgc cataagggtc agccttgcta
     2161 cgtgaacctt tggccggacg acgtgcacac gccctgggtg ccgggtcaag aaaggcttgg
     2221 tctctatccg agcggaccag tggaggagcg caagttccgt gcggtgctcg acgaatacga
     2281 ccgccaaatg ggccgcttac ttgaggggtt gaaacaactc ggcatcgagg ggcggacgct
     2341 ggtgatcttc accagtgata acggcccgct gccgagcttt cgcggcagcc ggagcggcgg
     2401 gttgcggggc agcaagctca gtctgtacga gggtggcatc cgtatgccgt ttatagtccg
     2461 ctggcccggg catgctccgg ccgggcggct ggatgaccag acggtgctgt ccgccacgga
     2521 cttgttcccc agcttgtgtg ccctggccgg tgtgaaactg ccgaagaacg ttgcatttga
     2581 tggcgaggac cttagtgccg cgttgcttgg cacccctatt gcccgtgcca aggccctctt
     2641 ctgggaatat ggccgcaaca ccaactcctt cgccttcccg aaaggcgctg accgcagccc
     2701 aaacctcgcg ctgcgcgaag gtgattggaa gttcctcatc aatgcggata gtaccgaccc
     2761 gcagctttat gatctcaaga gcgaccccag agaggccgcc aatctcacgt ctgcgaaccc
     2821 cgaggttgtg gaccggttga aggagaaggc gctagcttgg cgaaaagccc ttccgagaat
     2881 gaggctcaac gcgcctccga gtagcgctga ccgagaggct cgatgactag agtgaccaac
     2941 gaaaaggacc gtgtgacggg cggcgcgccc ggctcggaga acctggcggc ggcggagcgg
     3001 aagtttctgc ttgagctggc ccgggagacc ctcacgcgcg tcaccgccgg gggcagcctg
     3061 cccgaggtgg cgaccaaagc cgtgtcgccg aagctcaggg agaagaaggc ttgtttcgta
     3121 acgttgacca agaacggcgc actgcgtggc tgtatcggcc acctgacggc tatggagccc
     3181 ttacacgagg cggtggctga gaacgcccgc aacgcggcta tgcgcgaccc acgcttccca
     3241 ccgatcgaac ccggcgagtt ggaccagatc aagatcgaga tcagcgtact gacggaaccg
     3301 caaccgctgg ccttcagttc cccggacgac ttgctgagca aactgcaccc caactcggac
     3361 ggagttctgc tgcacctcgg cccgcgcacg gcgacgttct tgccgcaggt ctgggcgcag
     3421 gttcccgata aggtcgagtt cctgggccac ctctcgcaga aggccggctg cgaacgctcc
     3481 gcctggcggg gcaaggatgt ttcggtctcc atctaccacg tcgagtgctt tgaggaagag
     3541 cacgcccgtc cctgagcgcc agcggtgagg ggcggcacgt gatgattacc tgctcacatc
     3601 gcgaaatggt attcgaatac tcctcgctca accgtctgcc aattccgcgg cagcggcgtt
     3661 agacgcgacc tgtgccgggt ccttgcagcc ggggatcacg gctgagacga ctgggttctt
     3721 caagcaccac gccagcgccc actgtgccat tggcacaccg gcaggcagct cggtttggcg
     3781 gatacgttcc acttctgcca aatcctggcc catcttctca gccttgaacg tcgagcgcca
     3841 atcgttggcg gggaaggccg cgccgggacg gtacttgccg ctcaagaggc cgctggccag
     3901 cgggacgcgc gccaggatgc ccagattatg ctgctgggcc tgcgggaaat actgctgctc
     3961 gggccggcga tcaaggcggt tgtaaatcac ctgcaaggtc tccacaccca gacgctgcgc
     4021 ctcccgggcc tgcgcctcgc tgcctttgcc gagtatcgaa atccccagat gccggacttt
     4081 gcccgcgcgc ttctgctcag tcagcatccc ccacaggtcc tcattcagca gttggctgtc
     4141 tgagccggaa tggaattgat aaaggtcaat ggtctcgacg cgcaatgccc gcagcgacgc
     4201 ttcaagctgc ttgcgaacgt ccgccgctga gaaaccatcg gtgcggtcct ggaacttgtt
     4261 gaagtgatgg ccaaacttgg ttgccacgag ccagcgcgag cggtcgtgcc ggctaaggta
     4321 atcgccgatc agaccct
//
LOCUS       DATCGS010000057         3257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_55205, whole genome shotgun sequence.
ACCESSION   DATCGS010000057 DATCGS010000000
VERSION     DATCGS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55205"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VIL39_01160"
     CDS             complement(<1..220)
                     /locus_tag="VIL39_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5480066.1"
                     /translation="MSPDPQIVVQDLRLAYGRKEVIHGISFDIQRNEIFGIIGPAQSG
                     KTSLLRCLNRTIDFTPEARVSGAIQVDGE"
     gene            complement(354..1241)
                     /gene="pstA"
                     /locus_tag="VIL39_01165"
     CDS             complement(354..1241)
                     /gene="pstA"
                     /locus_tag="VIL39_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY5480067.1"
                     /translation="MMFEKTPFVRRKHRQETLAKWVFFSMAVSMIVPLILIVGYLVAR
                     AWPSLSLSFLIDVPKHGMAEGGIWPALVGTIYLVVISLAVAAPIGVLAAVYLNEYARD
                     NWFNRIINLAVINLAGVPSIVHALFGLGAFVYAARFGYSIISASLTLAIMTLPVIIAS
                     TREALASVPRSFREACWNVGATRWQTIKAVVLPNSISGILTGIILQVSRAAGETAPIM
                     FTGAVFYKAVQKGDLFPYGLREQCMALSMHLYTLATQVPGVKESIPFATAVVLLGSVL
                     FVNATAIIVRGYLRSRKKW"
     gene            complement(1238..2185)
                     /gene="pstC"
                     /locus_tag="VIL39_01170"
     CDS             complement(1238..2185)
                     /gene="pstC"
                     /locus_tag="VIL39_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY5480068.1"
                     /translation="MNTLATSKPVEQSLAVRKAVRPAWAVLGERALEVLIHLCGFSAI
                     IFVLGIFFFVFKEAFPILGHKQFSLSQFLFSEQWYPTSEVNKRYGVLAMIAGTFSVTA
                     LAMVIAVPFGLGTAIYLSEFCGSRVRETLKVVIELLAAIPSVVWGFVGLTVMSRLITK
                     YTGAPVGVNVLNGAIILALMSVPIIVSIGEDALKAVPDSYREAGLALGATRWQLVWRV
                     LLPAAKNGLLAAVLLGVGRGVGETMAVLMATGHAVHIPHSIFDSVRTLTANIAAELGE
                     APANSDHYHVLFLIGVLLFMMTFVVNLTADLIVRGIRRK"
     gene            complement(2294..>3257)
                     /locus_tag="VIL39_01175"
     CDS             complement(2294..>3257)
                     /locus_tag="VIL39_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5480069.1"
                     /translation="TTNRTKAMNQYQRYSAFLKLNAATTAATLAVCLLQTGCGKSEPS
                     GGGDAAKQQTIQNKGSDTMVNLAQAWAEEYKKAAPDVDVEVSGGGSGVGIAALEKGTI
                     NIANASRNMKPEEIEAAKKNTGKTPKEFIVGFDALAIYVHKDNPLNEITLDQIAQIYM
                     EGGALTKWSDLGVTIPGASGDEIVRVSRQSSSGTYEFLREHVLHNKDYKLGSRDMNGS
                     KEVVELVSSTPTAIGYSGMGYATPAVKMLKVKKTASDPAYEPSVANTLAKTYPIARSL
                     QVYTLGEPQGAVKKYIDWMMSGAGQKVVETSGYVPLPAEARAKP"
BASE COUNT          647 a          966 c         1003 g          641 t
ORIGIN      
        1 cctcgccgtc cacctggatg gctcccgaca cgcgcgcctc cggcgtgaag tcaatggtgc
       61 gattgaggca gcggagcagc gaggtcttgc ccgactgggc ggggccgatg atgccgaaga
      121 tttcattgcg ctggatgtcg aaggagatgc cgtggatgac ctccttgcgg ccataggcga
      181 ggcgcaggtc ctggacaacg atctgggggt cggggctcat gggaggagag agcggagtga
      241 tggagtgatg gagtggtgga gtaatggaat catggcgcgc gaagctggta ccctgagcag
      301 ccgtaccagc actccatcac tccaacactc cattactccg tgtgtcttca tcctcaccac
      361 ttcttccgcg atcgcaggta gccgcgcacg atgatggcgg tggcattgac gaacaagact
      421 gaaccgagca ggaccacggc ggtagcaaac gggatggact ccttgacgcc gggcacctgg
      481 gtggcaagcg tgtagagatg catcgagagg gccatgcact gttcacgcaa gccgtaggga
      541 aacaaatcgc ccttttgcac agctttgtag aagacggccc ccgtgaacat gatcggggcg
      601 gtctcgcctg ccgcccggct cacctgcagg atgatcccgg tgagaatgcc gctgatggaa
      661 ttgggcagca caacggcctt aatagtctgc cagcgcgtgg ccccgacgtt ccagcaggcc
      721 tcgcgaaaac tgcgcggcac actggccagg gcctcgcgcg tgctggcgat gatgaccggc
      781 agcgtcatga ttgccaacgt cagcgaggcc gatattatgg agtagccgaa cctggccgca
      841 taaacgaacg ctcccaggcc aaacagtgcg tggacgatgc tcggcacacc cgccagattg
      901 atcacggcca ggttgatgat gcggttgaac cagttatccc gggcatattc gttcaggtaa
      961 accgcggcca gcacgccaat cggcgcggcg acggcaagcg agatgacaac aaggtaaata
     1021 gtgccgacca gcgccggcca gatgccgcct tccgccatgc cgtgtttggg gacatcaatc
     1081 aggaagctca ggctgagaga cggccaggct cgcgcgacga ggtaaccaac gatgagaatc
     1141 aatgggacaa tcatcgagac cgccatgctg aagaacaccc acttggcgag agtctcctgc
     1201 cggtgcttcc gccgcacgaa tggagttttc tcaaacatca tttcctcctg atgccacgaa
     1261 caatgagatc ggcggtcagg ttgaccacaa aggtcatcat gaacaagagc acgccgatga
     1321 ggaacaacac gtggtaatgg tccgagtttg ccggggcttc acccaactcg gcggcgatgt
     1381 ttgctgtaag tgtccgcacc gagtcaaaga tgctatgcgg aatatgcacc gcatgccccg
     1441 tcgccatcag cacggccatc gtctcgccca ctccgcgacc gacgccaagc aggacggcgg
     1501 ccagcaatcc gttctttgcc gccggtagca gcacgcgcca gacgagctgc cagcgcgtcg
     1561 cgccaagggc caagcccgcc tcgcggtagg aatccggcac cgctttgagc gcgtcctcac
     1621 cgatcgaaac gatgatgggc acgctcatca gcgccaggat gatggctcca ttgaggacat
     1681 tcacgccaac gggggcgccg gtgtatttcg taatcagccg gctcatcacg gtgaggccga
     1741 cgaaccccca aaccacgctc ggaatggcgg ccagcagctc aatgaccacc ttcagggttt
     1801 ccctgacccg cgagccgcag aattcggaaa ggtaaatggc cgtgccgagg ccgaaaggca
     1861 cggcgatgac catcgccagc gcggttacgc tgaaggtgcc cgcaatcatc gccagcacgc
     1921 cgtagcgttt gttgacttcc gacgttggat accactgctc gctgaacagg aattgactca
     1981 ggctgaactg tttatggccc aggatgggga aggcctcctt gaagacgaag aagaagatgc
     2041 ccagaacaaa aataatcgca ctgaagccgc agagatggat gagaacctcc aaggcccgct
     2101 cgcccaggac agcccacgcc ggtcgcacag cctttcggac tgccaggctt tgctcaaccg
     2161 gtttggatgt tgccagagtg ttcattctgc ctgggagcga cccccgctcc ggccgggaac
     2221 tgcggtgctg gagagattgc tcttccatct cctgtggccc gaaatcccaa gcccaacggc
     2281 ccggcgcgag tgcttacggt ttggcgcgcg cttccgccgg cagcgggacg tagccgctgg
     2341 tctcgaccac cttctgtccg gcgccggaca tcatccagtc gatgtatttc ttaactgcgc
     2401 cctggggctc gcccagcgta tagacctgga gcgagcgggc gatgggatag gtcttggcca
     2461 gggtgttggc cacgctcggc tcataggccg gatcgctggc ggtcttcttc accttcaaca
     2521 tcttgacggc cggggtggcg tagcccatgc cgctgtagcc gatggccgtc ggtgtggagg
     2581 agaccagttc gactacttcc ttggaaccgt tcatatcccg ggaaccgagc ttatagtctt
     2641 tgttgtgcaa tacgtgttcg cgcagaaact cgtaagtgcc cgaactggat tggcggctga
     2701 cccgcacgat ctcatcgccg gatgcgccgg gtatcgttac gcccaggtcc gaccatttgg
     2761 taagcgcgcc gccttccatg tagatttgag cgatttgatc gagcgtgatc tcgttgagcg
     2821 gattgtcttt gtggacgtag atcgcgaggg cgtcgaagcc gacgatgaat tccttgggcg
     2881 ttttgcccgt gttcttcttg gcggcctcga tttcctcggg cttcatgttc cggctggcgt
     2941 tggcaatgtt gatggtgccc ttttccagcg ccgcgatgcc gacgcccgat ccaccgccgg
     3001 agacttccac gtccacgtcg ggcgccgcct tcttgtactc ctccgcccat gcctgggcca
     3061 ggttaaccat ggtgtcggag cctttgttct ggatggtctg ctgcttggcg gcatcaccgc
     3121 cgccgctggg ttcgcttttg ccgcagccgg tttggagcag acacacagca agggttgccg
     3181 ccgtggtcgc cgcgttgagt ttgaggaagg ctgaatatcg ttggtattgg ttcatagctt
     3241 ttgttctgtt tgtggtt
//
LOCUS       DATCGS010000058         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_450937, whole genome shotgun sequence.
ACCESSION   DATCGS010000058 DATCGS010000000
VERSION     DATCGS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450937"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VIL39_01180"
     CDS             <1..646
                     /locus_tag="VIL39_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480070.1"
                     /translation="IFPRLRTVIPYAVRCVGCEDAEELIQDATALAAGILHSAEAAGK
                     KVAASSVAYYAVQHCKSGRRSVGNSRVDVLAAGTQLNGHTRLTSLDEVAAANEECGGD
                     IYTFNDVLSDDQEDPGTKAARKMDWESFCAGLPERERVAVLFVAEGKTLREAARSLGV
                     SDSAMQGSKRSLGVKILEFMGADIMVEVRRRPQWRSNLEATRERQACRVLRQAA"
     gene            complement(816..2075)
                     /locus_tag="VIL39_01185"
     CDS             complement(816..2075)
                     /locus_tag="VIL39_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY5480071.1"
                     /translation="MKSRTGGLEWCGGNKPTGGVRPVKGVLPIALCARAQGKIGILVP
                     PENAAEAAVVSGLRVIPIRNLREAVSFLEGEVKIPPVKVDLARLFDQPPDGELDFAEV
                     KGQESVKRALEIAAAGGHNVLLIGPPGTGKSMLAKRLPTILPPLTLEEALETTKIHSI
                     VGLLSPGQALVTQRPFRAPHHTASDAGLLGGSVNPTPGEISFAHHGVLFLDELPEFKR
                     SVLETMRQPLEEGHVTISRAAGTMTFPSQFMLVAAMNPTPDGKMPAESRSSPREIQNY
                     LSRISGPLLDRIDLHIEVPPVKFREITSEQAGEPSAQIRERVVAARQRQHARFRDKPK
                     ITCNARMGSRELKSHCVLDEATMELLKFAMTDLNLSARAYDRVRSCQAIWSKGGPVPT
                     PLSLLEFGNSLAPRRFDGALRKREKRA"
BASE COUNT          421 a          648 c          710 g          428 t
ORIGIN      
        1 aatcttcccc cggctgcgca cggtcattcc ctacgccgtc cgctgcgtcg gctgcgaaga
       61 cgctgaggag ttgatccagg atgccaccgc cctcgccgcc ggcatcctcc acagcgctga
      121 agctgccggg aaaaaggtcg ccgcttcgag cgtggcctat tacgcggtcc agcattgcaa
      181 gtctggtcgc cgaagcgtgg gcaacagccg cgtggatgtg ctggcggctg gcacgcagct
      241 gaatggacat acccggttga cctcgctgga tgaggtggcc gccgccaacg aggaatgtgg
      301 cggcgatatc tacacgttca acgacgtgct gtccgatgac caggaagatc caggaacgaa
      361 ggccgcgcgg aaaatggatt gggaatcgtt ctgcgccggg cttccggaac gcgaacgggt
      421 agcggtgctg tttgtggccg aaggcaagac gctgcgagaa gcggctcgat cgctaggcgt
      481 ttctgattcg gccatgcagg gcagcaaacg gagcctgggg gttaagatcc ttgagttcat
      541 gggtgcggac atcatggtgg aagtccgtcg ccggccgcag tggcggagca atctggaggc
      601 gacccgggaa aggcaggctt gccgcgtgct gcgtcaggcg gcgtagaaat cataaagggg
      661 ggctgccgtc agggcgggct ctctttttta ggtatcaggt ctgattgggc aaagccttat
      721 gcgtcggttc gattccgacc ctcgcctcca agtactggcc aagtaatgca gaagtagtgg
      781 gcgagtacgc gccagtagtc ctgtcctgga tcggattatg ctcttttctc ccgcttcctg
      841 agcgcgccat cgaatctacg cggggccaag gagttaccga actccagtag ggaaagaggc
      901 gtcggaaccg gaccgccttt ggaccaaatc gcctggcaac ttcggacgcg gtcataagcg
      961 cgggcgctga ggttcaggtc ggtcatggcg aatttcagca gctccatcgt cgcctcatcc
     1021 aggacgcagt ggctttttaa ctcacggctg cccatgcggg cattgcaggt gattttgggc
     1081 ttgtcccgaa agcgcgcatg ctggcgctgg cgcgcagcga ccacccgctc gcgaatctgc
     1141 gcggacggct cgccggcttg ctcgctggtg atctcgcgaa acttcaccgg cggcacttcg
     1201 atgtgcaggt caatgcggtc gagcagcggg ccggagatgc gactgagata gttctgaatc
     1261 tcacgcggtg agctgcggga ttcggctggc atcttcccat cgggcgtcgg gttcatggcg
     1321 gcgacgagca tgaactggga gggaaacgtc atcgtgccgg cggcgcgtga aatagtgaca
     1381 tggccctcct ccagcggctg gcgcatggtt tccaggacgc tgcgtttgaa ctcgggcaac
     1441 tcgtccaaaa acagcacgcc gtggtgagcg aaggagattt cgcctggcgt gggattgacg
     1501 ctcccgccca gcaaaccggc atcgctggcc gtgtgatgcg gcgcccggaa cgggcgttgc
     1561 gtcaccagcg cctgaccggg cgaaagcaac ccgacgatgc tatgaatctt ggtggtttcg
     1621 agcgcctctt ccagcgtaag cggcggcagg attgtgggca gccgtttcgc cagcatcgac
     1681 ttgcccgtgc ccggcgggcc gatgagcaag acgttgtgcc caccagcggc ggcaatttcc
     1741 agcgcgcgct tgacggattc ctgtcccttg acctccgcga aatccaattc cccgtcgggt
     1801 ggctgatcaa agagccgcgc gagatcaacc ttcaccggtg gtatcttgac ttcgccttcc
     1861 aggaagctga cggcctcacg cagattccga atggggatga cccgcaggcc ggaaaccact
     1921 gccgcttcgg cggcattctc cggcggcacc aggatgccga tcttgccttg cgcgcgcgca
     1981 cacaaggcaa tcggtaggac gcccttgacg gggcgcactc cgccagttgg cttgttgcca
     2041 ccgcaccatt cgagtccacc ggtccgcgat ttcattgcat tatagacaat tacgccgcta
     2101 cccggcgaca cccaagactt tgggcgagca tcttcgtaaa aagagggtcg atatgcaact
     2161 atctatgacg caactggcta aaatactggg ctttggaatc actgaca
//
LOCUS       DATCGS010000059         4175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_351898, whole genome shotgun sequence.
ACCESSION   DATCGS010000059 DATCGS010000000
VERSION     DATCGS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351898"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VIL39_01190"
     CDS             <1..1517
                     /locus_tag="VIL39_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEY5480072.1"
                     /translation="FDAGHPEQEEEGGRVYEVRSPKSNGQSPRAEGRTSQLAELHFGW
                     GDIDFKRIHVGDKIWKTSDPELDRRLRQSYEGDTPRFQRPILMEVHGLAGKPLTLIAR
                     DELGHVVQLDSAMPLARAEKQPLTTARLREQLGRLGGTPFKLGELKNSLAGDVLLPVS
                     ELNRLRREAVRELESQRAQPKRWRIECGARNAECGVTDTTRSPIIPHSALRTPHLIVL
                     VRELGQLDAALKCGVETLYCEFEDPKKYREAVQLVRAARASCSDSQPSTLNPQLWVAP
                     PRVFKMGEDWTLKQVRSSEADGYLVRNYDHLAFFANERRIGDYSLNIANRLTADYFKN
                     KFGLERITASYDLNFTQLEALLQAAPPEWFEVTIHQHIPMFHMEHCVFCAFLTSGTDY
                     TNCGRPCDNHDLKLRDRVGAEHPVKADAGCRNTVFNALAQTGADYVSRMLALGVRHFR
                     IEFLNETPDQATQTITRYRQLLSGEISGSQLWRELKLLNQLGVTRGQIGSPARR"
     gene            1530..2033
                     /locus_tag="VIL39_01195"
     CDS             1530..2033
                     /locus_tag="VIL39_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEY5480073.1"
                     /translation="MNKPAKRLPFGLRTIALFEAAKGFLALAAAGGLLSLRHTDLHAV
                     TDAFLLRHGIDPERHYMRLFIESVAQATHHHEGQLAVLSLAYALIRLAEGYGLWQGKH
                     WAEWFAVISAGLYLPWEFMHFARHPRPFTAGIILFNLALIFYLGKLLARQRSRRKSRP
                     TAQSGSS"
     gene            2135..3043
                     /gene="prmC"
                     /locus_tag="VIL39_01200"
     CDS             2135..3043
                     /gene="prmC"
                     /locus_tag="VIL39_01200"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY5480074.1"
                     /translation="MTVLEAIQRSTEFLGKKGVDSPRLQTELLLAHLLKQPRMRLYLE
                     FERVLTPAEVDAFRELIRRRGQREPLQHIVGSTSFCGFEIAVNRDVLIPRPETELLAE
                     RGWTFLNQLSTINSQPSTALDFGTGSGCLAIALACKCPAAEVYAVDISPEALALARQN
                     AARHGVAERIRFLQGDGFAAVPEGARFDLIISNPPYIPSGEIASLQAEVRDYDPRRAL
                     DGGADGLDYGRRLAAECAPFLKPHGRVMLEFGDGQAEDLREILQQQMWIVEAVEEDYT
                     HRPRIMVARRSNSTADEPGAAGTQPG"
     gene            3129..>4175
                     /gene="murA"
                     /locus_tag="VIL39_01205"
     CDS             3129..>4175
                     /gene="murA"
                     /locus_tag="VIL39_01205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5480075.1"
                     /translation="MIKGSVPLHGEVAISGAKNAVLPIMAATLLTSEPCVIRRVPNLS
                     DVKFMGQILTWLGAQVRFEGDTVRIQARKIKGAGDYDLIRKMRGSICVMGPLLGRLKK
                     AAVSLPGGCVIGARPINLHLKGFEALGAKVTIESGYVHASAKRLVGSEMFLGGRAGPT
                     VLGTANVMMAATLAEGVTVIESAACEPEVVDLANFLSAMGAQISGAGSPTITITGVKQ
                     LHGAEHEVIPDRIEAATYAVAALATNGEVTLRGARADHLGAVLDKLEDAGAEIDRCGA
                     NITVRRKGRLKPVDITTLPYSGFPTDVQAQMMALMTLTPGISIITERIFESRFMHVSE
                     LTRLGADIEIEGPSA"
BASE COUNT          791 a         1282 c         1362 g          740 t
ORIGIN      
        1 tcttcgacgc cggtcatcct gagcaggaag aagagggagg aagggtgtac gaggtccgaa
       61 gtccaaagtc caacggccaa agtccaagag ccgaaggccg aaccagccaa ctcgccgagc
      121 tccacttcgg ctggggggac attgacttta agcgcattca tgtgggcgac aagatttgga
      181 agacgagcga ccccgaactg gaccggcgcc tgcggcagag ctacgaaggg gacacgccgc
      241 gattccagcg cccgatcctg atggaggtgc atggcctggc gggcaagccg ctcaccctca
      301 tcgcgcggga cgaacttggc cacgtggttc aactcgactc agcgatgccg ctcgcccggg
      361 ccgaaaagca accactcacg accgctcgcc tgcgagagca gcttgggcga ttggggggca
      421 cgccgttcaa actgggcgag ctgaagaatt ccctggccgg cgacgtgctg ctgcccgtca
      481 gcgagctgaa ccgcctgcgc cgcgaggcgg tgcgggaatt ggagtcccaa cgcgcccagc
      541 cgaagcgatg gaggatcgag tgcggagcgc ggaatgcgga gtgcggagtt acggacacca
      601 cgcgttcgcc aattattccg cattccgcac tccgcactcc gcacttgatt gtcctggtgc
      661 gcgaacttgg acagctcgat gcggccctga aatgtggcgt ggagacgctt tactgtgagt
      721 tcgaggaccc gaagaagtat cgcgaggccg tgcagctcgt tcgcgcggcc cgtgcgagtt
      781 gctcggactc tcaaccttca actctcaacc ctcaactatg ggtcgctcca ccgcgggtat
      841 tcaagatggg cgaggactgg acgctcaagc aggtccggtc cagcgaggcg gacggctatc
      901 tcgtccgcaa ctacgatcat ctggcctttt tcgcgaacga gcggcgcatt ggcgattact
      961 cgctcaatat tgccaaccgg ctcacggcgg actacttcaa gaacaagttc gggctggagc
     1021 ggatcacggc ctcttacgat ttgaacttca cgcagctcga agcgctcctg caagcagcgc
     1081 cgccggagtg gttcgaggtc actattcacc agcatatccc catgtttcac atggagcatt
     1141 gcgtgttctg cgcttttctc accagcggca cggactacac aaactgcggc cgcccgtgcg
     1201 acaaccacga cttgaaactg cgcgaccggg tcggggcgga gcacccggtc aaagcggacg
     1261 ccggctgccg caacacggtc ttcaacgcgc tggcgcagac cggcgcggat tatgtctccc
     1321 gcatgctggc cctgggcgtg cgccatttcc gaatcgagtt cctgaacgaa acgcccgacc
     1381 aagccacaca aacgattacg agataccgcc aattgctgag cggcgagatt agcggctcgc
     1441 aactctggcg tgaattgaag ctactgaacc agctcggcgt cacccgcggt cagattggct
     1501 cgccggcacg gcgataggac tatcccgaaa tgaacaaacc ggcgaaacga cttccttttg
     1561 gtctgcgcac cattgcgctc ttcgaggccg ccaaaggctt cctggcgctc gcggctgcgg
     1621 gcgggctgct gtccctgaga cataccgacc tccacgcggt taccgacgcg ttcctgctgc
     1681 ggcacggaat cgacccggag cggcattata tgcggttgtt catcgaatcc gtggcccagg
     1741 ccactcacca tcatgagggc cagctcgccg tcctgagctt ggcttacgcc ctgatccggc
     1801 tcgcggaagg ctacgggctg tggcagggca aacactgggc cgaatggttc gccgtcatct
     1861 ccgccggcct ctacctgccc tgggaattca tgcacttcgc gcgtcacccg agaccgttca
     1921 ccgctggcat cattcttttc aatctcgccc tgatcttcta cctggggaaa ctgctggcgc
     1981 ggcaacgctc tcgccggaag agccggccca cggcccagtc cggatcgtcg taagcaactg
     2041 cggccgaaga cggaagaccg ggagcgctgg cagatgcccg ggcgacgtcc cttttaggct
     2101 ttgaacgccg cgcacagtgc gtagccttgt cggcgtgacg gttcttgaag ccattcaacg
     2161 cagcacggag ttcctgggca agaagggggt tgattcgccc cggctgcaaa cggaactgct
     2221 gctggcgcat ctgctcaagc agccgcgcat gcggctgtac ctcgagtttg agcgggtgct
     2281 cacgccggcg gaagtggacg cctttcgcga actcatccgg cgccgcggcc agcgcgaacc
     2341 gttgcagcat atcgtcggtt ccacctcgtt ctgcggcttc gagatcgcgg tgaaccggga
     2401 cgtgctcatc ccgcgaccgg agacggaatt gctggccgag cgcggctgga ctttccttaa
     2461 ccagctatca actatcaact ctcaaccatc cactgcgcta gacttcggca ccggcagcgg
     2521 ctgtctggcg attgcgctgg cgtgcaagtg cccggctgcc gaggtctatg ccgtggatat
     2581 ttcgcccgag gcgctcgcgc tggcccgtca aaacgcggcg cgtcacggcg tggccgagcg
     2641 catccggttc ctgcaagggg acgggtttgc ggccgtgccc gagggagccc ggtttgacct
     2701 gatcatcagc aacccgcctt acatcccgag cggggagatc gcgtcgctgc aagccgaggt
     2761 gcgggattat gatccgcgcc gggcgctgga cgggggtgcg gatggcctgg attacgggcg
     2821 gcgcctggcg gcggagtgcg cgccgttcct caagccgcat gggcgggtga tgctggagtt
     2881 tggcgacggc caagccgaag acttgcgcga gattctgcag cagcaaatgt ggattgtcga
     2941 agcggttgag gaggactaca ctcaccgccc gagaatcatg gttgcgcggc gatcgaactc
     3001 aaccgcggat gaaccgggcg cggcggggac gcaaccaggg tgattcgtga tgcgtgacgc
     3061 gtgattcggt cacgccggag cgacaggaac ggcggtagtt agcagtataa ttttccatgg
     3121 acagcttttt gattaagggc tccgtgccgt tgcacgggga agtagccatt agcggggcga
     3181 agaacgccgt gctgccgatc atggcggcca cgttgctaac cagtgaaccc tgcgtgatcc
     3241 gccgggtgcc gaacctcagc gatgtgaagt tcatgggcca gatcctcacc tggctcggcg
     3301 cccaggtgcg ctttgagggt gacactgtcc gcatccaggc ccggaagatc aagggcgctg
     3361 gagattacga tttgattcgg aagatgcgcg gctcgatttg cgtgatgggc ccactgttgg
     3421 gccggttgaa gaaggccgcc gtgtcgctgc cgggcgggtg cgtgattggc gcccggccca
     3481 tcaacctgca cctgaaagga ttcgaggcgc tgggggccaa ggtaaccatc gagagcggct
     3541 atgtccacgc cagcgccaaa cggctggtcg ggtccgagat gttcctgggc gggcgcgcgg
     3601 gcccgacggt gctcggcacg gcgaacgtga tgatggcggc cacgctggcc gagggagtga
     3661 cggtgatcga gagcgcggcc tgcgagccgg aagtggtgga cctggcgaac tttctcagtg
     3721 ccatgggcgc gcagatttca ggcgcgggca gtcccaccat aaccatcacc ggcgtgaagc
     3781 aattgcacgg cgcggagcac gaggtgatcc ccgaccgcat cgaggcggcc acctatgcgg
     3841 ttgccgccct cgccacgaac ggcgaggtta ccctgcgcgg cgcgcgcgcg gaccacctgg
     3901 gagcggtgct ggacaagctg gaggacgcgg gcgcggagat cgatcgttgc ggcgcgaaca
     3961 tcaccgtccg ccgcaaaggc cgcttgaaac cagtggacat cacgacgctg ccctactcag
     4021 gcttccctac cgatgtgcaa gcgcaaatga tggctttgat gacgttgacg ccgggcatca
     4081 gcattatcac cgagcgaatc tttgagtcgc gcttcatgca cgtcagcgaa ctgacgcggc
     4141 tgggggcgga cattgaaatc gaaggcccga gtgcg
//
LOCUS       DATCGS010000060         3818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_648895, whole genome shotgun sequence.
ACCESSION   DATCGS010000060 DATCGS010000000
VERSION     DATCGS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648895"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="VIL39_01210"
     CDS             <1..2125
                     /locus_tag="VIL39_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY5480076.1"
                     /translation="KTLERIERNIGFRNIEVCGRQVYVNGQRVKLAGACHHEFDPLTG
                     RADTARHAETDVRLLKEANLNYIRTSHYPPTTELLDAADRLGMYVEVEAPFCWVGGAL
                     DTPTNLFATLTPTSAMVDYHHSHPSVMVWSIANESHFNLQFLASARLVKELDPTRPTT
                     FNHDFANSPSARVTDIANIHYPSMPYDDVLKDDPRPLFLGEYFFPICHEQTDVAIDPG
                     LRELWGFGHAEPDSAFSRACALDFDKPPLKPGLKPGGWSYIYHSDRVIGGAIWASHDD
                     AFYFSATKHAGYAWHHGFWGLIDYWRRPKPEWWLARHIFSPVWLETRHVPFVPGQKTV
                     RVPVENRYAFTDLSELKFNWQLNGHKGQLNPHLAPGTKGELEIPLPPGTAEGDRVSLR
                     VTGLSGALIQESSIQLGSEKPATLPRPASGAPRWSDDGHKLIIQGQGFAAVFDRAKGD
                     FDPADPRHTCPVSSFPTIHITRYDFGDLNGPNSPPYAVFPDAKTRRFEKIEVKEEASG
                     LRLVVHERYDGFAGSTSLLIDRDGRGVVACDYTYSGQPMNTREAGIRFLLKDACDTVR
                     WRRWSEWGVFPQESISRTEGTARAHRDGQWGEAHWNVRPAWPWSQDETELGTADFRSI
                     KYNLREAALVSRDGSGLSVYAGGDAHFRAALASDGVAAHLLWRCPLGQVPLKPNDRVQ
                     GEFVVGLTSGKASGSTASGTARGYR"
     gene            2295..2687
                     /locus_tag="VIL39_01215"
     CDS             2295..2687
                     /locus_tag="VIL39_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEY5480077.1"
                     /translation="METTESLGLRPATEESKAGGQGFVPKRLARERQTIAVMISCYCE
                     GHHHADTTLCPECRQLLDYAAVRLERCQFGAKKPVCAKCPVHCYERFRREQVKTVMSY
                     AGPRLLWQHPILSLRHWLDRFRKAPTVG"
     gene            2759..>3818
                     /locus_tag="VIL39_01220"
     CDS             2759..>3818
                     /locus_tag="VIL39_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480078.1"
                     /translation="MIVGRTAKFLAVAALALWMLLGDAASSCAGAAVPKQRSLDEWRS
                     LTAPPAPAPRVFVKGDHTRFYFPTATNVEEFDARWGRHRVPSDGYQVSSALLRWNQKL
                     ARIPEGQRGWREAKVVAGAEWSRLTTNLFEALTPTAPGHGAYYQGFLADRLLYRDAKG
                     APRFASIGEQPKEVIIDRHLSIDETLVTVAREVERDLVREHPGEALFMLLAPNARRFP
                     QPLLLDRQRRLCVWLSPAALYDTTERGIGLSTTAQGLSALLLEGHGLALLKNPISSVA
                     RLADLGVESVVRFVRLPFPKPSTHYPPLVRCQGMDLAQWEAWLDRYTGTRQEEGSLDL
                     LIDGEQFFPRLKQAIASAT"
BASE COUNT          662 a         1275 c         1161 g          720 t
ORIGIN      
        1 caagacgttg gaacgtatcg aacggaacat tggcttccgc aacatcgagg tctgcggccg
       61 tcaggtctat gtcaatgggc agcgcgtgaa gctggccggg gcctgccacc atgagttcga
      121 cccgctcacc ggccgcgctg acactgcgcg ccacgctgaa acggatgtcc gcctgctgaa
      181 ggaggcgaac ctcaactata tccgcacctc ccattaccca ccgaccaccg aactgctcga
      241 cgccgccgac cggctgggga tgtatgtgga ggtcgaagcc cccttctgtt gggtgggcgg
      301 cgcgctcgac accccgacca atctctttgc cacgctgacg cccacgtcgg ccatggtcga
      361 ttaccaccac agccacccca gcgtcatggt ctggtcgatc gccaacgagt cgcacttcaa
      421 cctgcagttc ctcgcctccg cccgcttggt gaaagaactg gaccccaccc gccccaccac
      481 cttcaatcac gactttgcca acagcccatc cgcccgcgtc accgacatcg ccaatatcca
      541 ttatccgtcg atgccctacg acgacgtgct caaggatgac ccgcgcccgc tattcctggg
      601 cgaatacttc ttccccatct gccacgagca aaccgatgtg gccattgacc ccggcttgcg
      661 agaactttgg gggttcggcc acgccgagcc ggactccgcc ttttcccgtg cctgcgccct
      721 ggacttcgac aaaccgccac tcaagccggg gttgaagccg ggcggctgga gttacatcta
      781 tcattcggac cgtgtcatcg gcggggccat ctgggcttcg cacgacgacg ccttctattt
      841 ctccgccacc aagcacgccg gctacgcctg gcatcacggg ttctggggtc tcattgacta
      901 ctggaggcgg cccaagcccg aatggtggct ggcgcggcac atcttcagcc ccgtctggct
      961 cgaaacgcgg cacgtgccgt tcgttcctgg gcagaagacc gtccgcgtgc cggtcgagaa
     1021 ccgctacgcc ttcaccgacc tcagcgaact caagttcaac tggcagctca acggccacaa
     1081 aggccaactc aacccccatc tcgcgcccgg cacaaagggc gagctcgaaa tccctctgcc
     1141 gccgggaacc gccgagggcg acagggtctc tctccgtgta accggccttt cgggcgcgct
     1201 aatccaagaa tccagcatcc agctcggcag cgagaagccc gccaccttgc cccggcctgc
     1261 ctccggcgca ccgcgctgga gcgacgacgg ccacaagctc atcatccaag gccaggggtt
     1321 cgctgccgtg ttcgaccggg ccaaaggcga cttcgatccc gccgacccgc gccacacctg
     1381 tccggtcagt tcgttcccca ccatccacat cacgcgctac gacttcggcg acctcaacgg
     1441 gccgaactcg ccgccttacg ccgtgttccc ggacgcgaag acccgccgct tcgagaagat
     1501 cgaagtcaag gaggaggctt ccggtctgag actggtcgtc catgagcgct atgacggttt
     1561 cgcgggctcc acgtccttgc ttatcgaccg cgatggtcgg ggcgttgtcg cctgcgacta
     1621 cacctattct ggccagccca tgaacacccg tgaggccggc atccgctttt tgctcaagga
     1681 tgcttgcgac accgtcagat ggcggcgctg gtccgaatgg ggcgtgtttc cgcaggagag
     1741 catcagccgg accgagggca ccgcccgggc gcaccgtgac ggccagtggg gtgaggccca
     1801 ttggaacgtg cggccagcct ggccctggtc gcaggacgaa accgagttgg gcaccgccga
     1861 cttccgctcc atcaaataca acctccgcga agcggcactg gtgtcgcgag acggttcggg
     1921 cttgagcgta tatgccggcg gcgacgccca tttccgtgcc gccctggcat ccgatggtgt
     1981 ggccgcgcac ctgctttggc ggtgtcccct gggccaagtc ccactcaagc cgaatgaccg
     2041 cgtccagggc gagtttgtgg tggggttgac atctggcaag gcatctggct caaccgcgtc
     2101 cggcacagcc cggggctata gatgatcaac ggattcgtag gctcgcttca tttctgctaa
     2161 gctctgtgcc cttgccggac cgggggtggc cacgccagtc gttggttttc ggagctcact
     2221 taattcctcg ctgctttgac ttgtatcaga gatactctgc tgcttatgtg cgaagattgt
     2281 gtcaattgac tgatatggaa accaccgaat cccttggctt gaggccagcg acggaagaaa
     2341 gcaaagctgg cggacaaggc tttgtcccca agcgtctggc ccgggaaagg caaacgattg
     2401 cggtgatgat tagttgttac tgcgagggcc atcaccatgc cgacacgacc ctttgcccgg
     2461 agtgccgcca gcttttggac tatgctgccg tccggctgga gcgctgccaa tttggggcga
     2521 agaagcccgt ctgtgccaag tgcccggtcc attgttatga gcgcttccgg cgcgagcagg
     2581 tcaagacggt gatgtcctac gccggtccac gcctgctgtg gcagcatccg atcctgagcc
     2641 tgcggcattg gctggatcgc tttcgcaagg cgccgacagt cgggtgaatc acgcgggctg
     2701 gtggcgggga tgcagcatcg ttgacttcct taggcccaac ttgttcactg tctgcaaaat
     2761 gatcgtggga cgcaccgcaa agttcctggc tgtggcggcg ctggccctgt ggatgctttt
     2821 aggcgatgca gccagctcat gtgctggggc ggccgtcccc aaacagcgct cgctggatga
     2881 atggcggagc ctgactgccc cgccggcgcc ggctccccga gtcttcgtca agggcgacca
     2941 tacccgtttc tatttcccga ccgctaccaa cgtggaggag ttcgacgccc gttggggccg
     3001 gcatcgtgtt cccagtgacg ggtatcaagt cagctctgcg ctgctccgct ggaaccagaa
     3061 gctggcccgc ataccggaag ggcagcgcgg ctggcgcgag gccaaagttg tcgccggagc
     3121 cgagtggagc cggcttacga cgaacctgtt cgaggcgctg acgccgacgg ccccggggca
     3181 cggcgcctac taccaggggt tcctggccga ccgccttctg taccgtgatg ccaagggcgc
     3241 gccccgattt gcctccatcg gcgagcaacc caaggaggtg atcattgacc gtcacctctc
     3301 gattgacgag accctggtga ctgtggcgcg ggaggtggag cgggatttgg tgcgggagca
     3361 cccaggcgag gcgctcttca tgttattggc accgaacgcc cggcgtttcc cgcaaccgct
     3421 gttgctggat cggcagcggc ggctgtgcgt ctggctgtcg cccgcggcgc tctacgacac
     3481 taccgaacgc ggcatcgggc tctccaccac cgcccagggc ctcagcgcgt tgctgctgga
     3541 agggcacggt ctggcgctgc tcaagaatcc catttccagc gtggcccgcc tcgccgattt
     3601 gggcgtggag tcggtggtgc ggttcgtgcg gctgccgttc cctaaaccca gcacgcatta
     3661 tccccccctg gtccgctgcc agggcatgga tctcgctcag tgggaggctt ggctggaccg
     3721 ttacaccggc acgcggcagg aggaggggtc gctcgacctg ctgattgatg gcgagcagtt
     3781 tttcccgcgg ctgaaacagg cgatcgcctc cgccacca
//
LOCUS       DATCGS010000061         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_698564, whole genome shotgun sequence.
ACCESSION   DATCGS010000061 DATCGS010000000
VERSION     DATCGS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698564"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..2483
                     /locus_tag="VIL39_01225"
     CDS             279..2483
                     /locus_tag="VIL39_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480079.1"
                     /translation="MKRRACRSPLKRSATWLVAAFLVTVGTGLAAAPSAASPRLTLAV
                     LAFEDQTADPEAAHWRYTVGRLLADQLQEAKAIRRVPAAFGYRQLKLKEGGPISAEQA
                     RKIGELIEARRVVWGSYRREGQQWLVTARVLNVASGAAGKELKAASADWHEVRDQLGD
                     QILNELGVKPSAPERQRMLRRGTRVPLALEWYSQTYAGQEQRQPKAEQESSIRKALAA
                     DPQFADAHVALAAVLGSQGRFESAEEAVRQAVKLRPDDASPHQVLGFALMFQTKFAEA
                     ETELRESLRLDPDNAETLARLGECSQVQGKLDEAIAFWSEAKQLDPTDAGVHAHLGDA
                     YAKKREREKALRELKEAERLDPEGVNSEQIVWQGYAALHETPLALEHLERFVALARKQ
                     GLAPEMVGDIAECGRELKARLTPHEISLAPPRSYTAQTLEATLRARLTRDEYQQVINP
                     LASTPAMDRWGQELTRGATNDLGKGKRIFDALARHLDTGDLGTRTAQEVFAAWNDPAQ
                     SFCCQEYAKLYVALARAAGIRAFFVHVEKDYSGEIVYHDCAAVWVGDKALLVDPAYQW
                     FGAPHKEYVVLDDVQAIAHHFYQPSPKGSPMARCRLAAKLHPETAWGQIRLASALIKA
                     NDLLQAAETLQIAQQLEPGRWDGYTCQGLLAIKRNDLDGAVVNLHKALAINPKHGPSR
                     FLLGWALAKQGKLEAARDEYRIALLYDSLLSSEDKATVLRAIANINEKLPAK"
     gene            complement(2458..>2871)
                     /locus_tag="VIL39_01230"
     CDS             complement(2458..>2871)
                     /locus_tag="VIL39_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480080.1"
                     /translation="TDVLTLDLVGQGCVAALPNLCTSEALVAAGRCQRVLSICVEVCS
                     AALYFDDDPGVPVSACLFGDGAGAAVLANSPGGRGGPCSGRPVAVLLDQQNAVSPDTP
                     EEVWTRLGGGPFHIGYTPVIYLALQALLTWLVASR"
BASE COUNT          578 a          880 c          966 g          447 t
ORIGIN      
        1 ggactgtttg cgatagctga atagctgagt tcaagggagc agtgtggaat caagatgctg
       61 tcagccacca ccaaggcggg cggccatgcc aatcggaacg ccggattcat ccggcaggcc
      121 cagtgtggct caccggcctg ccggatgaat ccggcgttcc ggtggcggtg tcaggatgca
      181 ccccgtgagg gctttcgctg cgcgcggggc ccgtttgcct tgaaaatctg gtggcgaaag
      241 gtagtgtagc cagcatgacg gcccccggtt ccattgattt gaagagacgc gcgtgccggt
      301 ctccactgaa gcgcagcgcc acctggctgg tggccgcctt cctggtgacg gttggaaccg
      361 gtctcgccgc cgcgcccagc gccgcgtcgc ctcgcctgac gctagccgtc ctggcattcg
      421 aggaccagac cgccgatccg gaagcagcgc attggcgcta caccgtaggg agattgcttg
      481 cagatcaact tcaggaggcg aaagcgatca ggcgagttcc ggctgcattc ggctaccggc
      541 aactgaagct gaaagaaggc ggcccgatca gcgcggagca ggcgcgcaag atcggcgaac
      601 tcatcgaagc ccggcgggtg gtttggggaa gttaccgccg ggaaggccag cagtggctgg
      661 tgaccgcgcg ggtgctaaac gtggcgagcg gcgcagccgg gaaggagctg aaggcggcct
      721 cggcggattg gcacgaggtg cgggaccaac tgggggacca gattctaaac gagctgggcg
      781 tcaaaccgag cgccccggag cgccagcgca tgctgcggcg cgggacccgt gtgccgctcg
      841 cactggaatg gtatagccag acgtacgccg ggcaggagca gcggcagccg aaggccgaac
      901 aggagtcaag catccgcaaa gccctggccg cggacccgca atttgccgac gcgcacgtgg
      961 cgctggcagc ggtgctggga agccagggcc ggttcgagtc agccgaggaa gcggttcgtc
     1021 aagccgtgaa gctgagaccg gacgatgcgt ccccccacca ggtcctgggc ttcgcgttga
     1081 tgttccaaac caagtttgcg gaggcggaaa cggaactgcg cgaatcgctg cgcctcgacc
     1141 cggataacgc cgaaaccctt gcgcgcctcg gtgaatgctc ccaggtgcag ggcaaactgg
     1201 atgaggcgat tgccttttgg agcgaggcca agcaactcga tccgacggac gcgggcgttc
     1261 acgcccacct gggggatgct tacgccaaga agcgcgagcg cgaaaaggcg ctgcgcgaac
     1321 tcaaggaagc cgagcgcctg gacccggaag gggtgaactc ggaacagatc gtctggcaag
     1381 gctacgccgc cctgcatgaa acaccgctgg ccttggagca cctggaaagg ttcgtggctc
     1441 tggcacgcaa gcagggcttg gcgcccgaaa tggtgggcga catagccgag tgcggccgtg
     1501 aattgaaggc ccggctcacc ccccatgaaa tcagcctcgc tccgcccagg agctacaccg
     1561 cacaaacact ggaggcgacc ctgcgcgcac ggctcacgcg agacgagtac cagcaggtca
     1621 tcaatccact cgccagcacg ccggcgatgg accgctgggg gcaggaactg actcgcggag
     1681 ccaccaacga cttgggcaag ggcaagcgga ttttcgacgc gctcgcgcgg cacctggata
     1741 ccggggattt gggcactcgc accgcccagg aggtgtttgc ggcctggaac gatccggccc
     1801 agtccttctg ttgccaggaa tacgccaagt tgtacgtggc gctggcgcgc gcggcgggca
     1861 tccgggcctt tttcgtccac gtggaaaaag actactccgg ggaaatcgtt tatcacgatt
     1921 gcgccgcggt atgggtcggg gacaaggcct tgctcgtgga tccggcctat cagtggttcg
     1981 gcgcgccgca caaggaatac gtggtgttgg atgacgtgca ggccatcgcc catcatttct
     2041 atcaaccatc gccgaaaggc agcccgatgg cgcgctgccg gctggcggca aagctgcatc
     2101 ccgagacggc gtgggggcaa atccgattag ccagcgccct gatcaaggcg aacgatttgc
     2161 tccaagccgc cgagacattg caaatcgcgc agcaactcga gcccggccgg tgggacggct
     2221 atacctgtca ggggttgctt gcgatcaaga ggaacgacct cgatggggcg gtggtcaact
     2281 tgcacaaggc gctcgcaatt aatccgaagc acggtccctc ccgattcctg ctgggctggg
     2341 cgctggccaa gcaaggcaag ttggaagcgg cgcgggacga ataccgcatc gccctcctgt
     2401 atgactcgtt gttatcttcc gaggacaagg cgaccgtgct gcgcgcgatt gcgaacatca
     2461 acgagaagct accagccaag tgagcagagc ctgaagagcg agatatataa cgggcgtgta
     2521 accgatatgg aacggaccgc ctcccaacct tgtccatacc tcctccgggg tgtccggtga
     2581 gacagcattc tgctggtcga gcagcaccgc cactggcctt ccactgcacg ggccgcctct
     2641 cccgccaggg gaattcgcga gcactgccgc acccgcgcca tcgccgaaca ggcaggcgct
     2701 gacgggcacg cccggatcgt cgtcgaagta gagggcggcg ctgcacactt ccacgcagat
     2761 ggacagcaca cgttggcagc ggccggcggc caccagggct tcgctcgtgc agagatttgg
     2821 cagggcggcc acacaaccct ggccgacgag gtcaagggtc agcacatccg t
//
LOCUS       DATCGS010000062         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_351930, whole genome shotgun sequence.
ACCESSION   DATCGS010000062 DATCGS010000000
VERSION     DATCGS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351930"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            743..2353
                     /locus_tag="VIL39_01235"
     CDS             743..2353
                     /locus_tag="VIL39_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY5480081.1"
                     /translation="MNLEAETNRRRTFAIISHPDAGKTTLTEKFLLYGGAVHLAGSVA
                     ARKNQRATTSDWMELEKKRGISVSSTVLQFEYRGYCINLLDTPGHKDFSEDTYRVLTA
                     VDAAVMVIDAGKGIESQTLKLFEVCRRRGVPIFTFMNKLDRPARAPLDLLDELERVLG
                     LHAYPVNWPLGDGQDFRGVYDRRSRQVHFFERVPGGAFRAPVTVHDLSDAAVRETMAP
                     DVYTRVVDELAMLEGAGAAFDQAKVLAGELTPVFFGSAVNNFGVQLLLDTFLDLAPTP
                     QPRVLKDRTVHPTTDGFSGFIFKIQANMDAKHRDRLAFVRICSGRFERDMTVIHTRTG
                     KRVRLASSHKLFGRARETVHEAFAGDVVGLVGHSEFRIGDTLAEDPTLVFDEIPRFAP
                     ENFAWIHSASTAQFKRFREGLNQLLQEGMAQLFLLHNATQRVPLLGVVGPLQFDVVQY
                     RLQTEYGAESRWEHGPWKLMRWVTAGKVDDTMLPSGARLATDAAGQTTILFTDEWSCN
                     FFAERNPSVTLATLPPQAGAGATAGKTL"
     gene            complement(2593..3552)
                     /locus_tag="VIL39_01240"
     CDS             complement(2593..3552)
                     /locus_tag="VIL39_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5480082.1"
                     /translation="MMNRRSFFTATACLGAAAALGAESQEHPPAHAVGRKRIKLGLCS
                     YSYWHFRDPKVSIETVIDKAAEFGVSGVDILHRQMDIPEKEPLTVEHRGYLQKLKRHA
                     FRNGIDLVCLSIHQNFVQQDPAERRKQVEHTHKCLEIAYELGIPCVRLNSGRWNTIKD
                     FDDLMKARGLEPILPGCTEDDGFKWCIECIEKCIPKAEQCGVVMALENHWGLSRTPEG
                     QLRLINAVPSPWLGALMDTGNFMEDPYDKLKLIAPKTVYVQAKTYYGGGEWYTLDLDY
                     RRIAKILFDAGYTGYCSLEFEGKESPDTAVARGFEVLRQTIGA"
     gene            complement(3549..4016)
                     /locus_tag="VIL39_01245"
     CDS             complement(3549..4016)
                     /locus_tag="VIL39_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480083.1"
                     /translation="MNTMTRGNQLTTKEKIMKTLAGLWIDHREAVIVILSDKGEETRR
                     VISHVEKQLRRSGRLPSQTPFEAQMMPADDSREREYSGHLANYYDEVISCLRPAKEIL
                     LFGPGEAKGELRKRIERNKLDVRITSCETSDKMTERQIAQKVRRHYLPSSTKP"
     gene            complement(4060..>4963)
                     /locus_tag="VIL39_01250"
     CDS             complement(4060..>4963)
                     /locus_tag="VIL39_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5480084.1"
                     /translation="MDTLLRAAKTPASILILGESGTGKSVVARAIHKQSHLADKPFIT
                     VSCPSLSRELLESELFGHVRGAFTGALRDHWGKVKAAAGGTLFLDEIGDLPMEIQPKL
                     LRLLQEREYERVGENVTRQAEVRVIAATSRDLRTRVAEGAFREDLYFRLNVIMAEMPP
                     LRQRPGDLIRFAGHYSKHFAALCGRRLEGFSEAALAAIRAYSWPGNLRELRNAIERAV
                     IMAKGNKINLEDLPAELRGQTAPGTKGNSASLEVGSLVSMEELAAAHLRIVLERTSNL
                     AEAAEVLGIDQATLYRKRKKIGLE"
BASE COUNT          910 a         1501 c         1500 g         1052 t
ORIGIN      
        1 aattgcttcg cagtccgatt catggcttaa gcaacgcacg aaaagccccc gcttgcacgc
       61 aagaagtggg gctttctaca gaactgcgtc aggcagtccc ggctgcgggt ggccagcgtg
      121 gcatcgaagc cccgggaagc gctcgggttt cggacgaact tatcgcgtcg ccgagaccgt
      181 ccagagactg tcaccaaccc ctgttttccc ttgattccct tcgtaacccc ttcgtaaccc
      241 cttcgaagcc ccttcgtaac tccttcgata catcgccggg gcagcgggag agcaacccgc
      301 tagcagcgcc ttagcagcaa gccagctagc caccccattc taccgtggca gaagccgtct
      361 cttgaggtaa cgcgcgccct ccaggcgagg ggtaaaacgc cccgcgccgg ttgacgttta
      421 attgccgctt gccacataca cggcaaatca tgttatgctc cggcctgtgg cattcacacc
      481 ggtatcagta attttgcgcc caacgcgcac ctgtctggcg gatcggggcg tcagcgcctg
      541 ccgtctgact ccaccgtagc gtaccgcacg ccgccggtgc aggcggttgc aagcgctatt
      601 ctccccgcgc ttcgcacgat ttgccccggc ctcctccgca attcggcggg tatttcattt
      661 cggcccttcg cgcccctttg aacccagccg cagaaaacag cctttagaac gttcccggga
      721 gaatgatgag ccctttgaac aaatgaatct ggaagcagaa acaaaccgcc ggcggacgtt
      781 tgcgatcatt tcgcatccgg acgcgggcaa gaccacgctc acggagaagt ttcttctcta
      841 cggcggcgcg gtgcatctgg ccggctcggt cgccgcgcgc aagaaccagc gcgccaccac
      901 gtccgactgg atggagttgg agaagaaacg cggcatctcg gtcagctcga cggtgttgca
      961 attcgagtat cgcggctact gcatcaacct gctcgacacg ccggggcaca aggatttttc
     1021 cgaggacact tatcgggtgc tgacggcggt ggacgcggcg gtgatggtga ttgacgcggg
     1081 caagggcatc gagagccaga cgctcaagct cttcgaggtc tgccggcggc gcggcgttcc
     1141 catcttcacc ttcatgaaca agctcgaccg accggcgcgc gctccgctgg acttgctgga
     1201 cgagctggaa cgggtgctgg gcctgcacgc ttatcccgtc aactggccgc tcggcgacgg
     1261 ccaggatttt cggggcgtgt atgatcgccg ttcgcggcag gtgcatttct tcgagcgcgt
     1321 gccgggcgga gcgtttcgcg cgccggtgac ggtgcatgac ctgtcggatg cggccgtgcg
     1381 cgagaccatg gcgccggacg tttacacgcg agtcgtggat gaactcgcca tgctcgaagg
     1441 ggcgggggcg gcgtttgacc aggcgaaagt tctcgcaggc gaactgacgc cggtcttctt
     1501 cggcagcgcc gtcaacaact tcggcgtgca actcttgctg gacaccttcc tcgatctcgc
     1561 gcccacgcca caaccccgcg tgctcaaaga ccgcaccgtt cacccaacga ccgatggctt
     1621 ctccggtttc atcttcaaga ttcaggcgaa catggacgcc aaacaccgcg accggctggc
     1681 attcgtgcgg atctgctccg ggcggttcga gcgcgacatg accgtgatcc atacgcgcac
     1741 cggcaagcgc gtccgcctgg ccagttcgca taagctcttc ggacgcgcgc gcgagacggt
     1801 gcatgaagcc ttcgccggtg atgtcgtggg gttggtcggc cattccgaat tccgcatcgg
     1861 ggatacgctc gccgaagacc cgacgctcgt ctttgatgag attccccggt ttgcgccgga
     1921 gaattttgcg tggattcaca gcgcgagcac cgcgcagttc aagcgcttcc gcgaaggact
     1981 gaatcaactg ctccaggagg gcatggcaca attgtttctc ctgcacaatg ctacccagcg
     2041 ggttcccctt ttgggcgtgg ttggcccgct gcaattcgac gtcgtgcaat accgtttgca
     2101 gaccgaatac ggcgcggaat cccggtggga acacggaccg tggaagctga tgcggtgggt
     2161 gacggcgggc aaggttgacg ataccatgct cccctccggc gcgcgcctcg caacggatgc
     2221 cgcgggccag accacaattc ttttcaccga tgaatggtcc tgcaacttct tcgccgaacg
     2281 gaatccgagc gtgacgctgg ccactttgcc cccccaagcc ggcgctggtg ctacggccgg
     2341 caagacgtta tagaacttct gagccacgtc cggcgtgact ctgaagcagg gcggggtggc
     2401 gcccgacacc gggttgtaag ttccgttaac ctggtcggac tcgcagagaa cccccgcttg
     2461 ccagatcaac tcgatgtcat tgccgttgcg ctgcacgatc aactgtctgg ccacttgagg
     2521 tgcaagacca ggtcgttggt cacccgcgcg aaggtgggcg gcggcatgac ggccagcctg
     2581 gccgcgacgc catcaggcgc cgatggtttg gcgcagcacc tcgaaacctc tggccacggc
     2641 agtatcgggg ctttctttgc cttcgaattc gagggaacag tagccggtgt acccggcgtc
     2701 gaagaggatt ttggcgatgc gccggtaatc cagatcgagc gtgtaccact caccgccgcc
     2761 atagtaagtc ttggcctgga cgtaaacggt tttaggcgcg atcagcttca gcttgtcgta
     2821 agggtcttcc atgaagttcc cagtgtccat gagggcgcca agccaggggg agggaacggc
     2881 gttgatgagc cggagttggc cttcgggcgt gcggctcagg ccccagtgat tttccagcgc
     2941 catgaccacg ccgcattgct cggccttggg aatgcacttt tcaatgcact cgatgcacca
     3001 tttgaagccg tcatcctcgg tgcaaccggg caggatcggc tcgaggccac gggctttcat
     3061 caggtcgtcg aagtccttga tggtgttcca gcgcccggag ttgagccgga cgcaagggat
     3121 gccgagttca taggcgatct ccaggcattt gtgggtatgc tcgacctgct ttctgcgctc
     3181 ggcgggatcc tgctggacaa agttctggtg gatagaaagg cagacgaggt cgatgccgtt
     3241 gcgaaaggca tggcgcttga gcttctgcag gtagccgcga tgctcaacgg tcaaaggctc
     3301 cttctccggg atgtccatct ggcggtggag aatatcaacg ccgctgaccc caaactcggc
     3361 ggctttgtcg atgaccgttt cgatggacac ttttggatcg cgaaagtgcc aataggaata
     3421 gctgcacagc ccaagcttga tgcgctttct gccgaccgca tgggcgggtg ggtgctcctg
     3481 gctttccgcg ccgagggccg cggctgctcc caggcaagcc gtcgcggtaa agaaagagcg
     3541 tctattcatc atggctttgt gctagacggc aaatagtgcc ggcgcacttt ctgcgcaatc
     3601 tggcgctccg tcatcttgtc gctcgtttcg cagctcgtga tgcgcacatc aagtttgttc
     3661 ctctcgatgc gcttcctcaa ctcgcccttt gcctcgcctg ggccaaacag cagaatctct
     3721 ttggccgggc gaagacagga aatgacttcg tcgtagtaat tcgccaaatg gcccgagtat
     3781 tctcgttctc gggaatcgtc tgctggcatc atctgcgcct caaaaggagt ctgggaaggc
     3841 aaacgaccgg agcgccggag ttgcttttcc acatgggata ttacccgcct tgtttcctct
     3901 cctttgtctg agaggatcac gatgacagcc tctcggtggt caatccataa accggctaat
     3961 gttttcatta tcttctcctt cgttgttaac tgattcccac gtgtcattgt attcatgatt
     4021 tcatttcttg ttggcgggtg tggtttcgga ccatcgcgat tactccaacc caatcttttt
     4081 gcgttttcgg tagagggtgg cctgatcaat ccccaggacc tcagccgctt cggccaggtt
     4141 ggaggttcgt tccagaacga tgcgaaggtg cgccgccgcc aattcctcca tggagaccaa
     4201 cgaaccgacc tccagtgaag cgctgttgcc cttggttccg ggagccgtct ggccgcgcaa
     4261 ttccgcgggg aggtcttcca ggttaatctt gtttcctttg gccatgatga ccgcgcgttc
     4321 gatggcgttg cgcagttcac gcaggttgcc cggccaggag taggcgcgga tagcggccaa
     4381 ggccgcttcg gaaaacccct cgagacggcg gccgcagagc gcggcaaaat gcttgctgta
     4441 atgcccggca aagcgaatga ggtcgcctgg gcgctgccgc agcggaggca tttccgccat
     4501 gataacgttg agccgaaaat acaggtcttc gcgaaaggcg ccctcggcga cgcgcgttct
     4561 aagatcgcgg ctggtggcgg cgatgacgcg cacctcggcc tggcgggtga cattttcgcc
     4621 cactcgttcg tattcacgtt cctgaagcag tcgcagcaac ttgggctgga tttccatcgg
     4681 caggtcgccg atttcgtcga ggaaaagggt tccgcccgcg gcggctttga ctttgcccca
     4741 atggtcgcgc aaggcgccgg tgaaggcgcc tctgacgtgg ccgaacagtt cgctttccag
     4801 cagttccctg gagaggctag gacagctcac ggtgatgaac ggtttgtccg ccagatggct
     4861 ttgcttgtga atggcgcggg ccaccacgct cttgccagtg ccgctttcgc ccagaataag
     4921 aatcgaggcc ggtgttttcg ccgctcgcag cagggtgtcc att
//
LOCUS       DATCGS010000063         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_451029, whole genome shotgun sequence.
ACCESSION   DATCGS010000063 DATCGS010000000
VERSION     DATCGS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451029"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1891)
                     /locus_tag="VIL39_01255"
     CDS             complement(440..1891)
                     /locus_tag="VIL39_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5480085.1"
                     /translation="MTNREHSSTPRILIVDDNPSIHRDFELVLLDNLEPPGLEADERR
                     MYGPAAQASFSKPACSLDHALSGLEGIEKVKQALAEDRPYQLAFVDIRLHGIDGVETI
                     ARVWRMDPRIQIVICTAYADYSWKDLARRLGRTDKLFLLKKPFDNIGVSLLASTLAEK
                     WFLARQAALKLEQMELLVAQRTQKVLALQRLEPPEPHEHSVLPAFPDAPPAGKELPLL
                     LLVEGDAEICRPIAQGLEADYRVIEAEDGEQGLRLAQETVPDIIILASSLPRLDGIGL
                     CRRLKSDELASHIPVILLAARGLEDFQIKALEAGADDCLAKPISLPLLKARVDNLLRS
                     RRKLHEHVSHELSLLPREVAANQLDAQFLRRTIFIIERHLSDFEFDIAALAQKLVVSR
                     RQLFRKLKAVAGSTPNVFIRSLRLKRAAQLLGETSMTVTEITYAVGFSDLKHFRSVFQ
                     EHFGVLPGDYARKAPPEEYAKNEPGTTGPGAKE"
     gene            complement(1899..3194)
                     /locus_tag="VIL39_01260"
     CDS             complement(1899..3194)
                     /locus_tag="VIL39_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480086.1"
                     /translation="MNLAQAASSRPTHARYWVIVFAVTLAIIQYVDKVCISQAAPFIQ
                     KDLHLREDQMGWVFGAFTLAYALFEIPAGYLGDRFGPRRVLLRIVIWWSFCTVALGRM
                     WSGGSLIVTQFLFGAGEAGAFPNLTKTFNQWLPPHERPRAQGIMWMSARLGGAFTPLL
                     VYLCLQYVTWRTAFLLFGLPGVVWAILFFRWFRDDPRSHPRVNAAEAALMPIQAGAGE
                     HFKAPWRKLFTSRTVWCLCGQYFACSYSFWFFITWFPTYLLKARGFDLKHSALLAGTP
                     LFVGAAGSLFAGWISPILSRQWGGVARVRRGIGAAGAFGAAALLVVPTWLTNPYMAVV
                     AIALVAFCNDIQLPGAWTACMDVGGKSVATLSGTMNMMGNVGGFVSPIVCGYLVKQTG
                     NWNLAFYVTAAAYVLGAFCWLGMDPVTPLEQQGRRTTTE"
BASE COUNT          723 a         1082 c         1041 g          562 t
ORIGIN      
        1 gggcgggcac ccgcgattca gcgggcgggc acaactccaa gcataccagc gcgcagtgaa
       61 gaccgaggag cggaatgcgg ccaggtattt tgctcacgaa cggcgaacag ccgcccgggc
      121 ggtgggacta gaccgcgtca agccacccaa actacagagc cacctgccac ttcaaaggca
      181 ccccaaggct accccaaggc cacccaaagg ccaccccaag gcctgttgat aggcaccgca
      241 taggcacccc aaagccaccc caaggctacc ccaaggctcc accaaagcca ccccaaggcc
      301 accccaaggc caccccaagg ctccaccaaa gccaccccaa ggctccccca gggctaccct
      361 gaggctacgc cggggcaaaa gctcccagca ggccgaatcg cggacacggc ggaggcggag
      421 cgggatcgct cgaacctaat cactctttgg ctccgggccc cgttgtgccg ggttcattct
      481 tggcgtattc ctccggcggg gcctttctgg cgtagtcgcc cggcagcacg ccaaagtgct
      541 cctggaacac gctgcggaag tgcttcagat cagagaagcc gacggcatag gtgatttcgg
      601 taaccgtcat tgatgtttct ccgagcagtt gagcggcgcg cttcaagcgc agggagcgaa
      661 tgaaaacatt gggcgtggag ccggcaacag ccttaagttt gcggaacaac tggcgacggc
      721 tgaccaccag cttctgcgcc agggccgcga tgtcgaattc aaaatccgag agatgccttt
      781 cgattatgaa tatggtgcgt cgcaggaact gcgcatcgag ctggttcgcg gccacttctc
      841 gcggcagcag gctgagttcg tggctgacgt gctcgtgcag tttgcggcgg gaccgcagca
      901 ggttgtccac ccgcgccttg agcaaaggca gactaatggg tttggcgagg cagtcgtcgg
      961 ccccggcctc gagcgccttt atctggaaat cttccaaacc gcgggcggca agcaggatca
     1021 ccggaatatg gctggcgagt tcatcgcttt tcaggcggcg acaaagcccg attccgtcca
     1081 gtcgcggcaa gctgctggcc aggatgatga tgtccggaac ggtttcctgc gccaggcgca
     1141 ggccctgttc accatcctcg gcctcaatga cccggtaatc ggcctccagg ccctgagcga
     1201 taggccggca aatctcggcg tcgccttcca ccagcagcag gaggggcaac tctttgccgg
     1261 caggcggggc gtctgggaag gcgggcaaca ccgaatgttc atgaggttcg gggggctcga
     1321 gccgctggag agccaggacc ttctgggtgc gctgggccac cagcagttcc atctgttcca
     1381 gtttgagcgc tgcctgcctg gccaggaacc acttttccgc aagggtgctg gccaacagac
     1441 tcaccccgat gttatcgaat ggcttcttga gcaagaatag cttgtccgtc cgccccaggc
     1501 gccgagccag atctttccag gaatagtccg cgtaggcggt gcagatgacg atttgaatac
     1561 gaggatccat ccgccatacc cgtgcaatcg tctccacgcc gtctatcccg tgcaggcgaa
     1621 tatccacaaa ggccaattga taaggccggt cttccgccag cgcttgtttg actttctcga
     1681 tgccttccag gccggaaagg gcatgatcca agctgcaggc gggctttgag aagctggcct
     1741 gggccgcggg cccatacatt cgccgttcgt ccgcttccag accgggaggt tcgaggttat
     1801 ccagcaagac caattcaaag tcccggtgga tgctcgggtt gtcatccaca atcaatatgc
     1861 ggggggtgct gctatgttcc cggttagtca ttgagcactc attctgtcgt tgtccgcctg
     1921 ccctgttgct cgagcggcgt gaccgggtcc atcccgagcc agcagaaggc gcccagcaca
     1981 taggccgcgg cagtcacata aaacgccagg ttccaattgc ccgtctgctt cacgaggtag
     2041 ccgcagacaa tcggtgagac gaatccgccg acgttgccca tcatattcat cgtgccggag
     2101 agggtggcga ccgatttgcc gcccacgtcc atgcacgccg tccacgcgcc gggcaattgg
     2161 atgtcattgc agaaggctac cagcgcgatg gcaactactg ccatataagg attcgtcagc
     2221 cacgtcggca ccacgagcag cgccgccgcg ccgaaggccc cggcggcgcc aataccccgc
     2281 cggactctgg ccacaccacc ccactgccgg ctgaggatcg gggaaatcca gcctgcgaac
     2341 agcgagcccg ccgcgcccac gaacagcggc gtgccggcca gcagcgcgct gtgcttcaaa
     2401 tcgaacccgc gcgccttgag caggtaggtt gggaaccagg tgatgaagaa ccagaaggag
     2461 tagctgcagg cgaagtattg cccacacagg caccagaccg ttcgcgacgt gaacagtttc
     2521 ctccacggcg ccttgaagtg ctcgcccgca ccggcttgta tgggcatcag tgccgcctcg
     2581 gccgcattga cgcgcgggtg cgagcgcggg tcgtcgcgga accaccggaa gaagaggatg
     2641 gcccacacca ccccgggcag gccaaagagc aggaacgccg tgcgccaggt gacgtattgc
     2701 aggcataggt acaccagcaa cggcgtgaag gcgccgccca gtcgcgcgct catccacatg
     2761 atgccctgcg cccgcgggcg ctcgtgcggc ggcagccatt gattgaaggt cttggtcagg
     2821 ttcgggaacg cgccggcctc ccctgcccca aacaggaact gggtcacgat gagcgacccg
     2881 ccgctccaca tccggcccag ggcgactgtg cagaacgacc accagatgac gatccgcagc
     2941 agcacccgcc gcgggccgaa gcggtcgccc aggtaaccgg cggggatttc gaaaagcgca
     3001 tacgccagcg tgaaagcgcc aaatacccag cccatctggt cctccctcaa gtgaaggtcc
     3061 ttctggatga agggcgcggc ctgggagatg catactttgt ccacgtattg aatgatcgcc
     3121 agggtcaccg caaagacgat cacccaatac cgcgcatgcg tgggccgcga tgaagcggct
     3181 tgagctaaat tcactgagga cgggttctcg ggaatcggct gcggcatctt ccaagggtag
     3241 taacgcgcag cgttgtccgc aatgagaaga tgagggggcg taaagcgtga aacgtgaagc
     3301 gtgaaacgtg aagcgtgaaa cgtgatgcgt gatgccgaga aaggagcggc acaaagctac
     3361 ccaaagccac cccaaggcta ccccaaagcc tgggcgggga aaccagct
//
LOCUS       DATCGS010000064         7558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_550544, whole genome shotgun sequence.
ACCESSION   DATCGS010000064 DATCGS010000000
VERSION     DATCGS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550544"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..937)
                     /locus_tag="VIL39_01265"
     CDS             complement(272..937)
                     /locus_tag="VIL39_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480087.1"
                     /translation="MKILDIPQSGKQGTFVSVQSRYGQYRRRYAVPRKSPSPAQTRTR
                     ATFGRVRALWRTLTEDQRAAWMTGNQDTSSEPRLGKSGRLPGYLLFMKVNATLACQGL
                     EPALTPTDRPSFDANPVAELVITNTGGAIDLKLSVSSAPATDILVLGTHPRSAGVTFA
                     KHFTILGRLPAAEAGYSNITQLYVDRYGVPPPGTRVFIRTRQVSNGWEDFPIQTTAIV
                     PKP"
     gene            complement(1121..2467)
                     /locus_tag="VIL39_01270"
     CDS             complement(1121..2467)
                     /locus_tag="VIL39_01270"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5480088.1"
                     /translation="MKMLKYIILVACYALAARFTNTSLHAGDNAPAAPPENKDTQAAK
                     PSAATPPSETENKPPPAATPTPGLLMTGLGAAGIAAPLTKAGISIYGYVEGGYMYDFT
                     APNGNAGPTYMGFNNFKNTAILDKISLNVERTVDPTKKAFDVGFRVEGIYGADAAFIH
                     SDGMMDSQTGRNQWDLLQAYVDVTFPNLPVRLRVGKWIELAGFEQFSANIYGAFGDPA
                     RALYSYSYQFLYAEPGTQTGALLTYVLNPQWTLDAGFTRGWNQSTRDANDALDFLGRV
                     TFTPSDKTSIIFVMTEGPEFPTGAGRNLPPGDNRDWWTALDLVITQKITDKLSLGLGF
                     DYVETPHIPGLPGGAKRWGGVAGYASYTIDPHFTLNTRLEWYEDAANGFSTGAPVSAS
                     YYAATLGVAIKPFPNDNIFSRLLFRPEIRYDYSDRPVFNGGDHNQWIFSVDALFTF"
     gene            complement(2550..3131)
                     /locus_tag="VIL39_01275"
     CDS             complement(2550..3131)
                     /locus_tag="VIL39_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5480089.1"
                     /translation="MKLERILLPLDIRKCPLEVFSVVNGLASHPGATVTLLYVVTLNI
                     AAPEKRVYERLARDAQWYLERLARGCLRPDIATNIRVRFGKPAEEILAEAATGNADLI
                     VLPSYPPSLWSRLFAPILPRVVEQVIRKAPCRVFLASPQDRFDCEDVWGRSGNKTHAG
                     SSQVPSALEREASRVSQAEDAMASTREQCRAVA"
     gene            complement(3367..3948)
                     /gene="kdpC"
                     /locus_tag="VIL39_01280"
     CDS             complement(3367..3948)
                     /gene="kdpC"
                     /locus_tag="VIL39_01280"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEY5480090.1"
                     /translation="MKELLSEFRRAACATLALAVVCCGLYPLIVFGIGQLLFPDKANG
                     SLIVDAGGVVRGSRLIGQPFAADKYFHPRPSSAGNGYDPTSSGGSNLGPTSQKLRDAI
                     AQNLADYRTQNGLATNAPVPADAVTGSGSGLDPHISSENARLQADRVAKARGLTPEHV
                     RELILQNTDHVSLGILGDPGVNVLRLNLALDRQ"
     gene            complement(3951..5999)
                     /gene="kdpB"
                     /locus_tag="VIL39_01285"
     CDS             complement(3951..5999)
                     /gene="kdpB"
                     /locus_tag="VIL39_01285"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY5480091.1"
                     /translation="MITKAPSLFDWNIVGPAIGDSFRKLHPRLMLKNPVMFVTMVGAA
                     LTTAGIGYCAAGERGFIAQLAIWLWFTVLFANFAEAVAEGRGKAQAASLRKARKDTMA
                     RRLRNGQEQKVPAPDLNKGDLVVCEVGDVIPADGDVVEGIASVDESAITGESAPVIRE
                     SGGDRSAVTGGTRVLSDRIVIRVTMDKGHGFLDRMIALVEGAQRQKTPNEIALTILLA
                     ALTVIFLLVCVTLKPFGIYSGVVFSVPVLIALLVCLIPTTIGGLLSAIGIAGMDRVLQ
                     RNVLAMSGRAVEAAGDVDVLLLDKTGTITLGNRMASAFIPAPGVPPERLADAAQLASL
                     ADETPEGRSIVVLAKQQFNLRGREVAELHARFVPFKAQTRMSGVDFSDEAKPASPVRS
                     IRKGAADSMRTYVDQLGGKFPPAVSQAVEEISRSGATPLVVAEGHEVLGVVQLKDMVK
                     GGIKERFAHLRKMGIRTIMITGDNPLTAAAIAAEAGVDDYIAQATPEMKLKRIRMEQE
                     AGHLVAMTGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEIVEIGK
                     QLLITRGSLTTFSIANDVAKYFAIIPAMLMVTFPAIAPLNVMHLHSPTSAILSAIIFN
                     ALIIVALIPLALRGVKYTPIGALAMLQRNLLIYGLGGVIIPFIGIKLIDLIITALNLV
                     "
     gene            complement(6038..>7558)
                     /gene="kdpA"
                     /locus_tag="VIL39_01290"
     CDS             complement(6038..>7558)
                     /gene="kdpA"
                     /locus_tag="VIL39_01290"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5480092.1"
                     /translation="SQMVGLVLHNFVSAATGIAIAAALVRGLARHSTKTLGNFWADLV
                     RTTYYLLVPICLVFAVVLVSQGMIQNFKPYTKVKLLEPYTTQVAKLDAKGQPVTTNVA
                     VMVQAPKLDDKGQPVLTNGVAMMVDVPKLDEKGQPVMTNMPVMVDQKVEEQAIVQGPM
                     ASQVAIKMLGTNGGGYTNANAAHPFENPTPLSNFLQMLAILAIGSGMTYYLGRMVKNQ
                     AHGWSVWAAMMALFLGGVLLCWYSEAAGNPIHHQLGVAAAGGNMEGKEVRFGIFNSAL
                     FATVTTVASCGGVNSMHDSFTALGGFVPLFNMQLGEVIIGGVGAGLYGMLVFVVLAVF
                     IAGLMVGRTPEYLGKKIQSYDVKMAMLALLVLALSILGFAAWASVSKWGLAGLNNNGP
                     HGLSEILYAFSSANGNNGSAFAGLSANTPWYNTTLGLAMLIGRFLMIVPIMALAGSLG
                     QKRIAPPSVGTFPVSGFTFVVLLIGTVLLVGALNYLPCLTLGPIVEHFLTAQGKLF"
BASE COUNT         1449 a         2173 c         2527 g         1409 t
ORIGIN      
        1 tggtcctact atggtccttg tatagtcctg ctatggtttt gctatggtcc tcataaacgg
       61 atctagtgtg gcaccataga ggctccaggg gctgaatctt actccgaaat cagaaacccg
      121 aagggcacga ggggctcgaa cggaaggaat ctggcggaga tagcccttgc agggcggagc
      181 gagagggtgg gatgccgaca cttcaccggc gcgggtcttt tggacacgct aagccggcaa
      241 gatgccggcg ctccgggttc tgatactcag gttaaggctt ggggacgatg gcggtggtct
      301 ggatggggaa atcttcccag ccgttgctga cctgccgggt gcgaatgaag acgcgcgtgc
      361 cgggcggggg cacgccgtac cggtcaacgt aaagctgggt gatgttgctg tagcccgcct
      421 ccgcggcagg caggcggccg aggatggtga aatgcttggc gaacgtgaca cccgcgctgc
      481 gagggtgggt gccgaggacc aggatgtccg tggccggagc actggagaca ctgagcttga
      541 gatcaatcgc gccgccggtg ttggtaatca cgagttcggc caccgggttg gcgtcgaagc
      601 tgggacggtc ggtcggagtg agcgcaggct cgaggccctg gcaggccaaa gtcgcattca
      661 ctttcatgaa gagcaggtag ccggggagac gtccggattt accgagacgg ggctcgctgc
      721 tggtgtcctg gttgcccgtc atccatgcgg cgcgctggtc ctcggtcagg gtgcgccaca
      781 gagcccggac gcggccaaag gttgcccgag tgcgggtctg ggcggggctg gggcttttgc
      841 gcggcacggc gtacctgcga cgatactgac cataccggct ctggacgctg acgaacgtgc
      901 cttgcttacc tgactggggt atatcgagta ttttcataaa ccaaggttct tatattccga
      961 gtgttgctca tcggttccgc caaacctcgg ccatccgccg ccccgattca agcggaaaat
     1021 cagtctctaa acggccagcc ctcgcggcgg cttaaggatt aaagagggat aagcactcaa
     1081 gatcgggagc ctccgagaag cagtgacacc gacgctgctc ctaaaaggtg aagagggcgt
     1141 ccacactgaa tatccactga ttgtggtcgc cgccgttgaa aaccgggcgg tcagagtagt
     1201 cgtagcgaat ctccggccgg aacagcaggc gggagaagat gttgtcgttt gggaacggct
     1261 tgatggccac gcccagagtg gccgcatagt agctggcgct gacgggcgcg ccggtggaga
     1321 agccattggc ggcgtcttca taccactcca gccgggtgtt cagcgtgaag tgcgggtcaa
     1381 tcgtatagct ggcgtatccg gccacaccgc cccaccgctt ggcaccgccc ggcaagccgg
     1441 ggatgtgcgg agtctcgacg tagtcaaagc ccaagccgag gctcaatttg tcagtgatct
     1501 tctgcgtgat caccaagtcc aaggcggtcc accagtcacg gttgtcgccg ggaggcaggt
     1561 tccgtccggc gcccgtcgga aactccgggc cttccgtcat cacgaaaata atggaagtct
     1621 tgtcgctggg tgtaaaggtc acgcgcccca agaagtccag agcatcgttg gcgtcgcgcg
     1681 tggactggtt ccagccgcgc gtgaagcctg cgtcaagcgt ccattggggg ttgagcacgt
     1741 aggtgagcaa agcgcccgtc tgcgtgcccg gctcggcgta gagaaactgg taactataag
     1801 aatagagcgc cctggccggg tcaccgaagg ccccgtagat gttggcgctg aattgctcga
     1861 agccggctag ctcaatccat ttgcccacgc gcagtcgcac cggcaagttg gggaaggtca
     1921 cgtccacata agcctggaga aggtcccact gattgcgacc cgtctgtgaa tccatcatcc
     1981 cgtccgagtg gatgaacgca gcatccgcac catagatgcc ttccacgcgg aagcccacgt
     2041 caaacgcctt cttggtcgga tcgaccgtgc gttcgacgtt caaactgatc ttgtcaagga
     2101 tggccgtgtt cttgaagttg ttaaatccca tgtaagttgg gccagcatta ccgttcggcg
     2161 cggtgaagtc atacatgtag ccgccttcga catagccgta gatgctgatc ccggctttag
     2221 tcaaaggggc ggctattcct gccgcgccca aacccgtcat caagagcccc ggcgtcggcg
     2281 tggccgcagg cggcggtttg ttttcagtct ctgacggagg cgtagctgcc gacggctttg
     2341 ctgcctgggt gtccttgttt tccggtggcg ctgccggtgc attgtcgcca gcgtgcagtg
     2401 atgtgttggt gaatctcgcc gcgagcgcat agcaggcgac gagaataatg tatttaagca
     2461 tcttcatgta tcaggctctt tcgttagttt atagagaaca tccgacaccg tcggaatagc
     2521 agtccctttt ctcaggaaag caggtcgtgt taggcgacgg cgcggcactg ctcgcgtgtc
     2581 gaggccatcg cgtcctcggc ctgcgaaacg cgcgaagctt cccgttccaa agcgctgggc
     2641 acctggctcg accccgcgtg ggttttgttc ccggagcggc cccaaacgtc ctcgcagtcg
     2701 aagcggtcct gcgggctcgc caggaacacg cgacagggcg ctttgcggat cacctgctcg
     2761 acgacgcgcg gcaggatcgg ggcgaagagg cggctccata aggacggagg ataggacggg
     2821 agcacaatca gatctgcatt cccagtggca gcctcggcca ggatttcttc agccggtttc
     2881 ccgaatcgga cgcgaatgtt ggtggcgatg tccggacgca agcacccccg ggccagccgc
     2941 tcaagatacc actgggcgtc acgggcaagc cgttcataga cgcgcttctc cggcgcggca
     3001 atgttcagcg ttaccacgta gagcaaggtc acggtggcgc cgggatggct ggccaggccg
     3061 ttgaccacgg aaaagacttc cagagggcat ttcctaatgt ccaaggggag aaggattcgt
     3121 tcgagtttca tcgcacacag tcacacagtc aattagccgg ccccgaagaa ctcaccgtct
     3181 tgaatggttt ggtttcaggt ggagcaggaa gtggcctagc cgtcgggcta ggcgcttgca
     3241 gagccgcacc agaggcccca ggaacaacca ccaccagata ggcagcgaga gcgcaaatcc
     3301 tgccgtcagc agaatagcca gcagaatgtc tatggcggac atagtcgtcc attggcgtcg
     3361 cggctgctat tggcggtcga gcgcgaggtt caggcgcagg acgttcacgc ccggatcgcc
     3421 cagaataccc agggagacgt ggtcggtgtt ttgcaggatg agttcgcgca cgtgttcggg
     3481 tgtcaggccg cgggccttgg cgacgcggtc ggcctggagg cgggcattct ccgagctgat
     3541 atgcgggtcg aggccgctgc cggagccggt gacggcgtcc gcaggcacgg gggcgttggt
     3601 cgccaggccg ttttgagtgc ggtaatcggc gagattttgg gcgatggcgt cgcgcagctt
     3661 ctgcgaggtc ggcccgaggt tgctcccgcc ggagctggtg gggtcgtagc cgttgcccgc
     3721 cgaggagggg cgcgggtgga agtatttgtc agcggcaaac ggctggccga tcaagcgcga
     3781 gccgcgcacc accccgccgg catccacaat gaggctgccg ttggccttgt cggggaagag
     3841 cagttggcca atgccgaata cgatcagtgg gtaaaggccg cagcagacga cggccagcgc
     3901 cagcgtagcg caggcggcgc ggcggaattc agagagcaat tctttcataa tcaaacaagg
     3961 ttcagggcgg tgatgatgag gtcaatgagc ttgatcccga tgaacgggat gatgacgccg
     4021 cccaggccgt agatgagcaa gttccgctga agcatggcga gcgcgccgat gggcgtgtat
     4081 ttgacgccgc gcaacgccag cgggatcaag gcgacaatga tgagggcgtt gaaaatgatg
     4141 gcgctgagga tggcgctggt cgggctgtgc aggtgcatga cgttgagggg cgcaatggcc
     4201 gggaatgtga ccatcagcat ggcgggaatg atggcgaagt atttggcgac gtcgttggca
     4261 atgctaaagg tggtgagcga gccgcgcgtg atgaggagtt gcttcccgat ctccacgatc
     4321 tcgatgagct tggtggggtt gctgtcgagg tccaccatgt tgccggcctc gcgggcggcc
     4381 tgggtgccgg tgttcatggc gacgccgacg tcggcctggg ccagggcggg agcgtcgttg
     4441 gtaccgtcac ctgtcatggc gacgaggtgc ccggcttcct gctccatgcg gatgcgtttc
     4501 aacttcattt ccggcgtggc ctgggcgatg tagtcgtcca cgcccgcctc ggcggcaatg
     4561 gcggcggccg tcagcgggtt gtcgccggtg atcatgatgg tgcgaatgcc catcttgcgc
     4621 agatgcgcaa agcgttcctt gatgccgccc ttgaccatgt ccttgagttg gaccacgccc
     4681 agcacctcat ggccttcggc cacgacgagg ggagtggcgc cggaacgcga gatttcctcc
     4741 acggcctggc tgacggcagg cggaaacttg ccgcccaatt ggtccacata cgtgcgcatg
     4801 gagtcagccg cgcctttgcg aatgctgcga acgggagaag caggcttcgc ttcgtcggag
     4861 aagtccacgc cactcatccg ggtctgggcc ttgaagggca cgaatcgggc gtgtagctcg
     4921 gcgacctcgc ggccgcggag attgaactgt tgcttggcga gcacgacgat cgagcggccc
     4981 tccggcgtct catccgcgag agaagccagt tgcgcggcat cggccaggcg ctccggtggc
     5041 acccccggcg cggggatgaa cgcgctcgcc atgcggttgc ccaaagtgat ggtgccggtc
     5101 ttgtcgagca gcagcacgtc cacgtctcct gcggcctcga ccgcgcgtcc gctcattgcc
     5161 aggacgttgc gctggagcac gcgatccatg ccggcgatcc cgatggcgct caacaagccg
     5221 ccgatggtgg tgggaatcag gcagaccagc agcgcgatca gcacggggac cgagaacacc
     5281 acgcccgagt aaatgccgaa gggcttcaac gtcacgcaga cgagcaggaa gatgaccgtg
     5341 agggccgcca atagaatggt gagggcgatt tcgttcggcg tcttctgccg ttgcgcgcct
     5401 tccactaggg cgatcatgcg atccaggaag ccgtggccct tgtccatcgt gacgcggatg
     5461 acgatgcggt cgctgagcac gcgcgtgccg ccggtgaccg cgctgcggtc gccgccactt
     5521 tctcggatga cgggggcgga ctcgccggtg atggcggatt catccacgct ggcaatgcct
     5581 tccacgacat cgccgtcagc cgggatgacg tcgcctacct cgcacacgac gaggtcgccc
     5641 ttgttcaagt ccggggcggg gaccttctgc tcctggccat tgcgcaggcg tcgggccatc
     5701 gtatccttgc gcgctttgcg caggctggcg gcctgggcct tgccgcggcc ctcggcgacg
     5761 gcttcggcga aattggcgaa gagcacggtg aaccagagcc aaatggccag ttgggcgatg
     5821 aagccgcgct cgccagcggc acagtaaccg atgcccgcgg tggtcagcgc ggcaccgacc
     5881 atcgtcacga acatgaccgg gttcttgagc atcaaccgcg gatggagctt gcggaaggag
     5941 tcgccgatgg cggggccgac gatgttccag tcaaataggg aaggagcttt tgttatcata
     6001 ggaaagatat tgcgtggtgc gtgttgcgtg ggccggacta gaagagtttg ccctgggcgg
     6061 tgaggaagtg ctcgacaatc gggcccagcg tcaggcaggg cagatagttc agagcgccga
     6121 ccaacagcac agttccaatc agcaggacga cgaaggtgaa gccggagacc ggaaaggtcc
     6181 ccacgctggg gggcgcgatt cgcttttggc cgagcgagcc ggccagggcc atgatgggca
     6241 caatcatcaa gaaccgaccg atcaacatcg ccagacccag ggtggtgtta taccagggcg
     6301 tgttggcgct aaggccggca aaggcgctgc cgttgttgcc attggcggag ctgaaggcgt
     6361 agaggatttc gctcaggccg tgcgggccgt tgttgttcaa tcccgccagt ccccacttgc
     6421 tcacagaagc ccaggcggca aagcccagga tggaaagtgc caagacgagc agcgccagca
     6481 tggccatctt cacgtcgtag gattggatct tcttcccgag gtattccggc gtccgcccga
     6541 ccatgaggcc ggcgatgaac accgccagca cgacgaaaac caacatgcca tagagcccgg
     6601 cgccaacgcc gccgatgatg acttcaccga gctgcatgtt gaacagcggc acaaaaccgc
     6661 cgagcgcggt gaaggaatcg tgcatcgagt tgaccccgcc gcaggaagcg acggtggtca
     6721 cagtggcgaa cagggcagaa ttgaagatgc cgaagcggac ttccttgcct tccatgttcc
     6781 cgcccgcggc ggccacgcca agctggtgat gaatcggatt cccggctgct tccgaatacc
     6841 agcagagaag caccccgccc aggaacaagg ccatcatcgc cgcccaaacc gaccagccgt
     6901 gggcctggtt cttcaccata cgcccgaggt aataagtcat cccgctgccg atggcgagga
     6961 tggcgagcat ctggaggaag ttggacaggg gcgtggggtt ctcgaagggg tgcgcagcgt
     7021 tggcattggt atagccgccc ccattggtgc cgagcatctt gatggcgacc tgcgaggcca
     7081 ttggcccctg gacgatggcc tgttcctcca ctttttgatc aaccatgacg ggcatgttgg
     7141 tcatcaccgg ttgccctttc tcatcgagct tcggcacgtc caccatcatc gccacaccat
     7201 tggtcagcac cggctggcct ttgtcgtcaa gtttaggcgc ttgcaccatc actgcgacgt
     7261 tggtcgttac cggctggccc ttggcgtcga gcttggccac ctgcgtagta tagggttcca
     7321 gcagcttgac tttggtgtaa ggcttgaagt tctggatcat gccttgcgac acaaggacga
     7381 ccgcgaagac caggcagatg ggcaccagca ggtaataggt cgtccgcacg aggtccgccc
     7441 agaagttgcc cagggttttg gtcgagtgcc gagcgaggcc gcgcacgagc gccgccgcga
     7501 tggcgatgcc ggtggcggcc gagacgaagt tgtgaagaac gagcccgacc atctgcga
//
LOCUS       DATCGS010000065         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_6128, whole genome shotgun sequence.
ACCESSION   DATCGS010000065 DATCGS010000000
VERSION     DATCGS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6128"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3167
                     /locus_tag="VIL39_01295"
     CDS             <1..3167
                     /locus_tag="VIL39_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEY5480093.1"
                     /translation="SVLDPISSLRRIVILAPHETARVDLVMGMAENRDAALALIEKYY
                     NPRMTDRALDLAWTHSQVTLRHLNASEADAQLYARLASPLIYASPARRASPSVLLNNR
                     RGQSGLWGYGISGDAPIVLLRISDSARIELVQQLLQAHSYWRMKGLPVDLVILNEDVS
                     VYRQPLQDQIFRLIASGIEDQMLDKPGGIFVRRLEQVSSEDQVLLQSVARLVLTDENG
                     SLTEQLERSGLPEPILPMLAPTRSSRRDSPEPLPQRDLVFYNGLGGFTPDGREYVITL
                     SADQVTPAPWVNVLANPYFGTLVSESGSAYTWVENCHEFRLTPWNNDPVTDVTGEAFY
                     IRDDQTGRFWSPTPLPARGATPYVIRHGFGYSVFEHTENGIASELTVYVAMDAPVKFA
                     VLKLRNVSRRPRRLSVTGYWEWVLGDLRHKNLLHVQTEVDPKTGALLARNPFNTEFAE
                     RIAFVDVSDSTRILTGDRKEFLGQNGSLSNPAALKRMRLSGKTGAGLDPCAAMQVTLD
                     LPAGQQLETTFRLGVGRSLADVQTLIRRFRPTEASRGALEGVWEYWKRTLGAVQVESP
                     DPSVNVMANGWLLYQTLSGRLWARTGFYQSGGAFGFRDQLQDVMALVHAEPALTRQHL
                     LRAAAHQFREGDVQHWWHPPTNRGVRTHCSDDYLWLPYATCRYVACVADTGVLDESVP
                     FLEGRPLKPEEDSYYDLPNRSEASGTLYQHCVRAIEYGLKFGQHGLPLMGGGDWNDGM
                     NLVGKDGRGESVWLAFFLYDVLTRFAELARRHNDTAFAERCLAQAHQLQQNIEQQAWD
                     GEWYRRAYFDNGEPLGSRTNPECQIDSLPQSWSVISRAGDPQRARQAMRAVDARLIRR
                     DAGLIQLFDPPFDLSSLNPGYIKGYFPGVRENGGQYTHGAIWTTMAFAQMGDTDRAWD
                     LFALLNPVHHSATPKQVATYKVEPYVVAADVYGVAPHTGRGGWTWYTGSAGWMYRLLL
                     ETLLGVNLEGDQLRLTPSFPKAWHTFTLHYRYRQTQYHIRITRLPDSPPGSSQLTLNG
                     QPLAGNILPLLDDHQDHSVGLQIS"
     gene            complement(3390..>3844)
                     /locus_tag="VIL39_01300"
     CDS             complement(3390..>3844)
                     /locus_tag="VIL39_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5480094.1"
                     /translation="LMLKTAANPGGLPIEVFDGIRLGSTTDRSQFYKDLASGPFFGAN
                     RPDAKVSQGMIDSFWLQGMQAGHKNTFDCIKAFSETDFTEDLKKFDVPTLILHGDDDQ
                     IVPIGAAALASSKLVKNSTLKIYAGAPHGLTETHKDQLNADLLAFLKA"
BASE COUNT          723 a         1260 c         1092 g          769 t
ORIGIN      
        1 gttccgtctt ggatcctatc agctcattgc ggcgcatagt catattggcc ccccacgaaa
       61 ccgcccgcgt tgatctggtc atgggcatgg cggagaaccg ggacgcagcg ctggcgctca
      121 tagaaaaata ctacaacccg cgcatgaccg atcgggcgct ggacctcgcg tggactcaca
      181 gccaggtcac cctgcgccac ctcaatgcct ccgaagccga cgcgcaactc tatgctcgac
      241 tagcgagtcc cttgatttac gccagcccgg cccggcgtgc ctctcccagc gtattgctca
      301 acaatcgccg cggccaaagc ggactttggg gctatggcat ctccggtgac gcgccgatcg
      361 tcttgctgcg catcagcgac tccgccagaa tcgagctcgt ccagcaactc ctccaggctc
      421 actcctactg gcggatgaaa ggtttgcctg ttgacctggt gattctcaat gaagatgttt
      481 ccgtctatcg ccagcccttg caggaccaaa tcttccgttt gattgcctcg ggcatcgaag
      541 accaaatgct cgacaaaccg ggcggcattt ttgtccggcg cctggaacag gtctccagtg
      601 aagaccaggt gctgctccaa tcggtcgcgc ggctggtcct gacggatgaa aacgggtcgt
      661 tgaccgaaca actggaacga tccggcctcc ctgagccgat ccttcccatg cttgcgccta
      721 cccgttcctc gcggcgcgat tcgcccgagc cgctcccgca gcgagacttg gttttttata
      781 atgggctcgg cggtttcacc ccagacggac gtgaatatgt cattacgttg tcagcggacc
      841 aagtcactcc cgccccctgg gtgaacgtgc tggccaaccc ctatttcggg acgctcgtct
      901 cagagagcgg cagcgcctac acctgggtcg agaattgcca tgagtttcgc ctgacgccct
      961 ggaacaatga tcccgttacc gacgtgactg gtgaagcctt ctacatccgg gatgaccaga
     1021 cgggacgatt ctggtcaccc accccgctgc ccgcgcgcgg tgcgacaccc tacgtcatcc
     1081 gtcacggctt cggctattcc gtgttcgaac acaccgagaa cggtatcgcc tccgagctga
     1141 ccgtttatgt agccatggac gcgcccgtga agttcgccgt cctgaaactg cgcaacgtct
     1201 cgagacggcc tcggcgtttg tccgtcaccg gctactggga atgggtgctg ggagacttgc
     1261 gccacaaaaa cttgctgcac gtccaaaccg aggtggaccc caagaccggc gccctgctcg
     1321 cacgcaatcc tttcaatacc gagttcgccg agcgcatcgc gtttgtggac gttagtgatt
     1381 ccacgcgaat cctcacgggg gatcggaaag agtttctcgg ccaaaacggg agtctatcca
     1441 atccggcagc cctcaagcgg atgcggcttt caggaaaaac aggagcgggg ttggatcctt
     1501 gcgccgcgat gcaagtgacg cttgatttgc ccgccgggca gcaactggaa accaccttcc
     1561 gcctcggggt ggggcggagc ctcgctgacg tgcaaaccct gatccgccgc tttcgtccca
     1621 cggaggccag tcgtggcgca ctcgaaggag tctgggaata ttggaaacgg acgcttggtg
     1681 ccgtgcaggt cgaaagcccc gatccctccg ttaatgtgat ggccaacggc tggctgttgt
     1741 atcagacctt gagcggccgc ctatgggctc gcaccggttt ctaccaatct ggtggcgctt
     1801 tcggcttccg tgaccaattg caggatgtga tggcgctggt gcatgccgag ccagccctca
     1861 cccgccaaca tctcttgcgc gctgcggcgc atcagttccg cgagggcgat gtgcagcatt
     1921 ggtggcatcc tcccaccaac cgtggcgtgc gcacgcattg ctccgatgat tacctgtggc
     1981 tgccttacgc cacctgccgc tacgtcgcgt gcgtggccga caccggcgtg ctcgacgagt
     2041 ccgttccctt tctcgaaggc cggcccctca agccggagga ggattcatac tatgacctgc
     2101 ccaatcgctc cgaagcatcg ggcacgctgt atcaacattg cgttcgcgcc atcgaatacg
     2161 ggcttaaatt cggccagcac ggcctgcccc tcatgggtgg cggcgactgg aacgacggga
     2221 tgaatctggt gggtaaagac gggcgcggcg aaagcgtctg gctggctttc ttcctctacg
     2281 acgtgctgac ccgatttgcc gaattggccc gccggcacaa cgacaccgct tttgccgagc
     2341 ggtgccttgc ccaagcccat caactgcaac agaacatcga gcaacaggcc tgggacggcg
     2401 aatggtatcg ccgcgcttac ttcgacaacg gagaacccct cggctcccgc accaatccag
     2461 aatgtcagat cgattccctg ccgcagagct ggtcggtcat cagccgcgcc ggtgatcccc
     2521 agcgcgcccg ccaggccatg cgagccgtgg acgcgcgcct cattcgccgc gacgccgggc
     2581 tgatccagct attcgatccc ccgttcgacc tatcctccct caacccgggc tacatcaagg
     2641 gctatttccc cggcgtgcgc gaaaatggcg gccaatacac tcatggcgcc atctggacca
     2701 ccatggcctt tgcgcagatg ggagatactg accgggcttg ggacctgttc gccctgctca
     2761 atcccgtcca ccacagcgcc acccccaagc aagtcgccac ctacaaagtc gaaccctacg
     2821 tcgtggccgc cgacgtctat ggcgtcgcgc cccatactgg ccgtggcggt tggacctggt
     2881 acaccggttc cgctggctgg atgtatcggc tcctgctcga aaccctgctg ggcgtgaatc
     2941 tggaaggcga tcaactgcgc ctcaccccca gcttccccaa ggcgtggcac acgttcaccc
     3001 tccactaccg ctatcggcaa acccagtacc acattcgcat taccaggctc ccagacagcc
     3061 ctcccggcag cagccaacta accctcaacg gtcagccgct cgctggcaac atcctcccct
     3121 tgctggacga ccaccaggat cattccgtgg gactgcaaat cagttaaatt ggggagggct
     3181 aaagctggcc gttggcccga accgggttcc tttgttcttc cggttccttc ggcatcttct
     3241 cggcccaagc ctccaatctc ggatcaaggg ggttggcctg cttatgctcc tggaccgctt
     3301 ggcgcagagc tttggactct ccatgtttcg aaaactaacc ggtacggtcc ccagtaccac
     3361 caagtccagc cggcgccggc gaagcgacct caggccttga ggaatgccag cagatcggcg
     3421 ttgagctggt ctttatgcgt ttcggtcagg ccatggggcg cgcccgcgta gatcttcagg
     3481 gtcgagttct tgacaagttt cgaggaggct aaagccgcgg cgccgatcgg cacgatctgg
     3541 tcgtcgtcgc cgtgtaggat cagcgtcggc acgtcgaact tcttgaggtc ttcggtgaaa
     3601 tccgtctcag agaacgcctt gatgcagtcg aaggtgttct tgtgcccggc ctgcatcccc
     3661 tggagccaga acgaatcgat catgccctgc gagaccttgg cgtcgggcct gttggcccca
     3721 aaaaatgggc cgctggcgag atccttataa aactgcgagc ggtcggtggt agagccgaga
     3781 cggatgccgt cgaacacctc aatcggcaag ccgccgggat tggcggcggt cttcagcatc
     3841 agcg
//
LOCUS       DATCGS010000066         5279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_451621, whole genome shotgun sequence.
ACCESSION   DATCGS010000066 DATCGS010000000
VERSION     DATCGS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451621"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VIL39_01305"
     CDS             <1..586
                     /locus_tag="VIL39_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480095.1"
                     /translation="YFFIILPLQCVKASGGLIRDELQADTLSFLVTRPLSRARLLLLK
                     YLTQTAWLQLVLLAQTLLLFAVGGLRQIPSLGALLPLFLAAQFLAVLTWSALGVFLGQ
                     VAKRYMAVALVYGFIVELGIGRIPTNINTLSMMRHLKTLLSHNPALQGIYDWSATGVP
                     MAVGALALATALFLTLAALLFTFKEYHQTAEMQK"
     gene            complement(618..2918)
                     /locus_tag="VIL39_01310"
     CDS             complement(618..2918)
                     /locus_tag="VIL39_01310"
                     /inference="COORDINATES: protein motif:HMM:NF039200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HEY5480096.1"
                     /translation="MQPSIKFLSSVLLLVVSSASQWAHGAPGVPNCAVNDVIWTTPGK
                     NENDSMPIGNGDVAANVWTEQNGDVVLLVAKSDAWTELGKLVKLGRVRIKLSPNPFGS
                     EARFEQKLALEKGAIEIKSGANTVQVWIDANHPVIHVETHLARPGTLQASLELWRTTA
                     RPFETPSPERGGLFEFGGHRLSINFEPDTVLPTRPDRLAWCHFNSNSVYPLVLQQEHL
                     GALLVKYPDPLLHRCFGAALSGPGLVSRDDHTLQSSTPGQELRLDLYALTQEQAASPR
                     SWETNLDALVKEVDRVKIGQARQAHERRWNEFWNRSWIHVEGTTDAANVSQSYCIQRW
                     MIACSSRGAQPAKFNGGLFTVGHDMAEGRDSKTADHNPDFREWGNSFWNQNTRLLYWP
                     LIATGDSDLLKPWFEMYLKALPLAKDRTRLYFHHDGAAFIETIHFWGLPNLNDFGWDN
                     PSNELSSSWIRYHTQGAVEVVMQMLDSYDYSQDAGFARSSLVPFADAIVTYYDQHWSR
                     DANGKLTLSPAQSLETYQMNAVNPTPDIAGLTAILPRLLALPDEVTSPQQRTSWARLL
                     HDLPPIPVGKTSHGKIPSAGKGDDDGMLVILPAEKYGKPSNSENPELYVAFPYRLYGV
                     GKPNLDLAQRTYAARLFPQNTCWGQDGTQASVLGLTTEAQKAAISEFTAYGNQRFIWF
                     WRAGHDWIPDLDNGGSGMITLQQMLMQCDGKRIQLLPAWPKEWTADFKLHAPYQTTVQ
                     GHVEGGKITDLKVAPESRAKDVEVVH"
     gene            complement(3018..3917)
                     /locus_tag="VIL39_01315"
     CDS             complement(3018..3917)
                     /locus_tag="VIL39_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5480097.1"
                     /translation="MLGVDFKFGAHQFLWKSHWTDRDLAILDQARSLGLTLFEISLGD
                     DVGFDRARLRRHAESLGIELTVGPGNLWPEDCNLSADDARHRRLGLAWHKKIVEQAAE
                     LGAAAYCGAHYGHPGQVCRRRPPAEEMPRVAENLRALAEYAGRLGVRLVIEPMSRFRT
                     HLVNTAAQTVNLVRMADHPNLSVNLDTYHMITEERDYGAAIRGALPVLWGVHTCENDR
                     GVPGGGLVPWMAVFNALSEAKGCVRLMLETYNTGPEGFGWSRGLFQNLCPDPEAFVRQ
                     GTAFLRGSIAAALRNDSIRVAQL"
     gene            complement(4032..5129)
                     /locus_tag="VIL39_01320"
     CDS             complement(4032..5129)
                     /locus_tag="VIL39_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY5480098.1"
                     /translation="MKPLIRFCLVASLALVAGCQTGSVDSSLSKADSTKSRRQEDAAL
                     RGVHLLLGSDSQAAALQEQLPRLAAAGVNTLILETDYNFEFKSHPELSSPGCVTQARA
                     HELAAEARRLGIRLIPDINCLGHQSWSKTTLALLKNYPEFDETPGQYPNNTNIYCRSW
                     CPQNPEVNKVVFALVDELVNAFEADAFHVGMDEVFLIASEHCPRCRGGDPAKLFAQAV
                     NDLHHHIVEERKWEMYMWADRLLDARTMGYGEWESARNGTAGAVDLISKDIVLCDWHY
                     ELRTNYPSVPFLVGKGFRVWPAGWKSLPAARALSEFSRGQQSKRVVGYLCTTWGAVRI
                     PSLAEWPPLVEVMKEWSMTPLPPRPPSAFRL"
BASE COUNT         1013 a         1603 c         1572 g         1091 t
ORIGIN      
        1 ctattttttc atcatcctgc ccttgcaatg tgtcaaggcc agcggcgggc taatccggga
       61 tgagttgcag gccgacacgc tcagcttcct ggtcactcgc cccctcagcc gggcgagact
      121 gctgttgctg aagtatttga cgcaaaccgc ctggctgcag ctcgtgctgc tggctcagac
      181 actgctgcta tttgccgtcg gcggtctgcg ccaaattccc tcactgggcg cgctgctgcc
      241 gctgtttttg gccgcgcagt tcctggcggt gctgacctgg agcgcgttgg gtgttttcct
      301 gggccaggtc gcgaagcgct acatggccgt ggcgctggtt tacggtttca tcgtcgagtt
      361 gggcatcggc cgcattccta ccaacatcaa taccctctca atgatgcgcc atctcaagac
      421 cctgctctcg cacaacccgg ccctgcaagg gatttacgat tggagcgcca cgggcgtccc
      481 catggcggtg ggggcgctgg cgctggccac cgccctcttc ctgaccctcg ccgcgctgct
      541 gttcacgttc aaagaatacc atcaaaccgc cgagatgcag aagtgaagca ggtgaacaag
      601 accgcgaacg gtaaggctta gtggacaacc tcgacgtctt tggccctcga ttccggagcg
      661 accttaagat ctgtaatctt gccgccttcg acgtggcctt gcaccgttgt ctggtatgga
      721 gcgtggagtt tgaagtcggc cgtccattcc ttcggccagg caggcagcag ttgaatgcgt
      781 ttgccatcgc attgcatcag catctgctgc agcgtgatca tcccggagcc gccgttatcg
      841 aggtccggaa tccaatcgtg gccggccctc cagaaccaga taaaccgctg gttgccgtaa
      901 gccgtgaact cgctgatggc cgccttttgc gcctctgtcg tcagccccaa gaccgaggct
      961 tgggttccgt cctgccccca acaggtgttc tgagggaaga gccgggcggc ataggtcctt
     1021 tgggccagat ccagattggg tttgccaact ccatagagac gataaggaaa ggcgacgtaa
     1081 agctccgggt tctctgaatt gctgggtttg ccgtattttt cagccggaag aatcaccagc
     1141 atcccatcgt catcaccctt tccagcggag ggtattttgc catggctcgt tttgccgaca
     1201 gggattgggg gcagatcgtg gagcagtctg gcccaggaag tgcgctgttg cggagaggtg
     1261 acttcgtccg gcaaggccag caggcgcggc agaatcgccg taagcccggc gatgtcaggc
     1321 gtggggttta ccgcgttcat ttggtaggtc tcgagggatt gcgccgggga gagcgtgagc
     1381 ttcccgtttg cgtcgcggct ccagtgttga tcgtaataag tgacgatggc atcggcaaaa
     1441 ggaaccagag aagagcgggc gaagccggca tcctggctat agtcgtagct atccagcatc
     1501 tgcatgacga cttccacggc gccttgtgtg tggtagcgga tccaggagct gctcaattca
     1561 ttggagggat tgtcccaacc gaaatcgttg agattgggaa gcccccagaa gtgaatggtt
     1621 tcgataaatg cggccccatc gtggtgaaag taaaggcggg tgcgatcctt ggccagcggc
     1681 agggctttga ggtacatctc aaaccagggc ttgaggaggt cgctatcgcc ggtggcaatc
     1741 agcggccagt aaagcaggcg agtgttctga ttccaaaaac tgttgcccca ttcccgaaag
     1801 tcgggattat ggtcggcggt cttggagtct cttccctctg ccatatcgtg tcccaccgta
     1861 aagaggccgc cattgaactt tgccggctga gcgccgcggg aggaacaggc tatcatccac
     1921 cgttggatgc agtagctttg ggaaacgttc gccgcgtccg tcgttccttc aacgtgaatc
     1981 caactgcgat tccaaaactc gttccaccgt cgctcatgcg cctgccgcgc ctggccgatc
     2041 ttcacccggt ccacctcctt gaccagggcg tccaggtttg tctcccaact ccggggggac
     2101 gcggcttgct cctgggtgag cgcatataag tcgaggcgca actcctggcc gggagtggag
     2161 gattgaaggg tgtgatcatc cctgctgacc aggcccggcc cgctcagcgc cgccccgaag
     2221 caacggtgca aaagcggatc ggggtacttt accaggagcg ctcccaaatg ttcctgctgc
     2281 aagaccaatg gataaacgct gttcgaattg aagtggcacc aggcaaggcg atccggccgg
     2341 gtcgggagga ccgtatcggg ctcgaaattg atggaaaggc ggtgcccgcc aaactcaaaa
     2401 agaccacctc gctccggtga gggagtctca aaaggacggg cggttgtgcg ccagagttct
     2461 aacgaggcct ggagagtccc tggacgcgcc aaatgagttt cgacgtggat cactggatga
     2521 ttggcgtcga tccaaacctg gaccgtgttg gcgccgctct tgatctcgat ggcgcccttt
     2581 tccagtgcca gcttctgctc gaacctcgcc tcgctgccaa aggggttggg cgatagcttt
     2641 attcgcacgc gcccgagctt aaccagcttt cccaactccg tccacgcatc ggactttgcc
     2701 accagcagca ctacatcgcc attttgctcg gtccaaacat tggccgcaac gtctccgttg
     2761 ccaatcggca tggagtcgtt ttcatttttc ccgggcgttg tccagatcac gtcgttaacg
     2821 gcacaattcg gaacgcccgg ggcgccgtgc gcccactggc tagccgaact gaccacgaga
     2881 agaaggacgg aagacaggaa ctttattgag ggttgcatat cgcttttctt gctcatttgc
     2941 gtttggttcc gaagtagttc atggccgctt cggtccgcga ccgaagcagc gaaagcagcg
     3001 gatctctttg ccgggcatca taactgagcg acgcggatcg agtcgttgcg caaagccgcc
     3061 gcgatggagc cgcgcaggaa ggccgtgccc tggcgcacga atgcttccgg atcggggcag
     3121 aggttctgga aaaggccccg cgaccaacca aacccttccg gcccggtgtt atacgtttcg
     3181 agcatcagcc gtacgcaccc cttggcttcg ctcagtgcgt tgaacaccgc catccagggg
     3241 acgagccccc cgcccggcac tccgcggtca ttctcgcagg tgtgaacgcc ccacaagacc
     3301 ggcaacgcac cacggatcgc ggcgccataa tcgcgctctt ccgtgatcat gtggtaagta
     3361 tccagattca cgctgagatt ggggtggtcg gccatgcgaa cgaggttcac ggtctgggcg
     3421 gcggtgttga ccaagtgagt tcggaaccgg ctcattggtt caatcaccaa ccgcacaccc
     3481 aagcggccgg cgtattcggc cagcgcacgg aggttctccg ccacccgcgg catctcctcc
     3541 gctggcggac ggcggcggca tacctggccg ggatgaccgt aatgcgcgcc gcaatacgct
     3601 gcagccccaa gctccgcggc ctgctcgacg attttcttat gccatgccag cccgagccgg
     3661 cgatgccggg catcatcggc ggagaggttg cagtcctcag gccagaggtt gcccgggccg
     3721 acggtcagct caatgcccaa cgactccgca tgccggcgca ggcgcgctcg gtcaaacccc
     3781 acgtcgtcac cgagtgaaat ctcgaagagc gtcaacccca gcgagcgcgc ctggtccagg
     3841 attgccaaat cgcggtcagt ccagtggctc ttccacaaga actgatgcgc gccgaacttg
     3901 aaatctacac caagcatcac aactggctcc atgctatgaa ggaacggtgg ccgtgcacat
     3961 cccctttttc gggccggtgt cggtcctcac tcttgatact ttccagagca gaacctcggt
     4021 ggtggtagcc gctagagtct gaaggctgaa ggcggcctcg gtggcagcgg cgtcatgctc
     4081 cactccttca tcacctccac cagtggcggc cactcggcga gcgaaggaat cctcacggct
     4141 ccccaggtcg tgcaaagata gcccaccacc cgcttgctct gctgcccgcg cgagaattcg
     4201 ctcaaggccc gggcggcggg caatgatttc cagccggcgg gccatacccg aaaacccttc
     4261 cccaccagaa acggcacgga aggataattg gtccgcaact cgtagtgcca gtcgcacagg
     4321 acgatgtctt tggaaatcaa atccactgct ccagccgtgc cgttccgggc cgattcccat
     4381 tctccgtagc ccatcgtccg ggcgtcgagc aggcggtcag cccacatata catctcccac
     4441 ttccgctcct ccacgatgtg gtggtggaga tcgttgaccg cttgggcgaa gagcttcgcc
     4501 gggtcaccgc cccggcagcg cggacagtgt tctgacgcga tgaggaagac ctcatccatg
     4561 ccgacatgaa atgcgtccgc ttcaaaggcg ttgaccaatt cgtccacgag ggcgaagacc
     4621 actttgttca cttccgggtt ttgcggacac cagctccggc agtagatgtt ggtgttgttc
     4681 ggatactggc cgggcgtttc atcgaactcg ggataattct ttaacagcgc caaggtcgtc
     4741 ttgctccagg attggtggcc gagacaattg atatccggaa tgaggcggat gcccagccgg
     4801 cgcgcttccg ccgcgagttc atgcgcccgg gcctgggtga cgcaaccggg cgaactgagt
     4861 tcaggatggg acttaaactc aaaattgtaa tccgtctcca gtatgagggt gttcacgccc
     4921 gcggcggcga gcctcgggag ctgttcctgc agcgcggccg cttgcgagtc gcttccgagc
     4981 agcaggtgaa caccacgcaa ggcggcatcc tcctgccggc gggattttgt gctatcagcc
     5041 tttgaaagac tggagtccac gctcccagtc tggcagccgg ccaccagcgc cagcgaggca
     5101 acgaggcaga atcggatcaa cggtttcatg ctgtgtctgg agattagcag gcgactcagc
     5161 ccgttcaagc ccgccatcgg cccttggcct ctcgcagtct tcgcgtcttc acaacgcaat
     5221 actccaaatt gttcgcaagc tgcgctgttg gttgtcggcg gctttggtcg ctgcggcga
//
LOCUS       DATCGS010000067         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_699110, whole genome shotgun sequence.
ACCESSION   DATCGS010000067 DATCGS010000000
VERSION     DATCGS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699110"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VIL39_01325"
     CDS             complement(<1..483)
                     /locus_tag="VIL39_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY5480099.1"
                     /translation="MKLRRFNWIISDLLRKALKRYFEWSARLKGQRFYCRALGGESDY
                     NLTINCDLTVSCNCQDYDGSGHIGNLNKNSFKEVFFGPVARRFREELAKGKLPIMTCT
                     RCGDLQRVPKSQSRAVTGVPGGVGRAASEAETDPDGQIFAGPKPRLPHRGLLLENTVR
                     C"
     gene            complement(480..1751)
                     /locus_tag="VIL39_01330"
     CDS             complement(480..1751)
                     /locus_tag="VIL39_01330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5480100.1"
                     /translation="MKLLVFAHTPPPHHGQSYMVQLMLAGFGGDRRQRVRRAADGGPE
                     ARFGIECYHVNARLSRTLEDIGEIQSIKLLRIFLFCLEAIWCRFRYGVDSFYYVPAPG
                     KRAALYRDWMVMFICRPFFKRIILHWHAAGLAKWLETSVQIRTRAVTYHAFKRPALSV
                     VISKYNVADAEKLFSQRICRVNYGIPDPCCDFEKTVLPRRKARFAARAKLFRNEPLGP
                     TDLEGTGGDPQIVRVLYLAHFMREKGLFAAAQAVVLANQILSRRKCPVQMKLFAAGTF
                     VTDEEKLEFDERLKNPEFAGAVACLGFVSGLEKERALREADLYCFPTRYPAENQPVTL
                     IEAMAFGLPIVTTRWRSIPEMFPPGYPGLVADQSTGPIADALLALMTVEIGEELRGIF
                     LRSFTLERYLTGLAEAFAQVEAPRPVTLFPL"
     gene            1878..2663
                     /locus_tag="VIL39_01335"
     CDS             1878..2663
                     /locus_tag="VIL39_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY5480101.1"
                     /translation="MTALELLGTPLLLTDYRGLDELCRQWARAGACVALDFANTQIVT
                     MRRHEPWFRDLTSAYDCFPPDGMPLIWCLRLAGARLRDRVYGPTFMRQFLIGAPAEAT
                     HYLLGGSAACGAQLRAVFGKLNPGLRFVGAFHGKCEADGRMEGAADKAVIEEINRLSP
                     DFIWVGFGTPKQQAWVKRYKSRLRRGVILTVGFAFDVNAGMKPDAPPWMQRLGLTWVY
                     RLASEPRRLASRYIRYNTLFLFYLLWDGLRGRAWTRSSGSTPR"
     gene            2733..3152
                     /locus_tag="VIL39_01340"
     CDS             2733..3152
                     /locus_tag="VIL39_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY5480102.1"
                     /translation="MKLSVKSDYAARAVLGLARHYPSQVALTTDQMAEEQGLPPKYLP
                     QILLELKAKDIVRSVRGKAGGYLLTRPPAEITLGDVLQAVDGPLFDAPGLSSSGGPPE
                     LRRAWQKLRQAVENAVGAITFQQLLAEAAEKEKMYYI"
     gene            complement(3167..>3452)
                     /locus_tag="VIL39_01345"
     CDS             complement(3167..>3452)
                     /locus_tag="VIL39_01345"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480103.1"
                     /translation="KLCEGMEDILSEFEYLFPSSNRPPSAGETGVLPALELSENEQKV
                     YDRLGNEEVGIDAVIRHSGLPSSAVSVALLNLEMKRLIRQLSGKLFVRNS"
BASE COUNT          675 a         1094 c         1046 g          637 t
ORIGIN      
        1 gcagcgcacg gtattctcca gcagcaggcc ccgatggggg agacggggct tgggaccggc
       61 gaagatttgc ccatcgggat cggtctccgc ttcgctggcg gccctgccaa ctcccccagg
      121 caccccggtc acagcccggg actgagattt cggcactcgc tgcaaatcgc cacagcgggt
      181 gcaggtcata atcggcagct tccccttggc cagctcctcg cgaaaccgcc gggcgaccgg
      241 gccaaagaaa acctccttga atgagttctt gttcagattg ccgatatggc cggagccgtc
      301 gtagtcctgg caattgcagg agaccgtcaa atcgcagttg atggtcaggt tgtaatccga
      361 ctcacccccg agcgcccggc agtaaaaccg ctggcctttg agccgggccg accattcgaa
      421 ataccgcttc agcgctttgc gcaaaaggtc gctgatgatc cagttaaatc ttctaagctt
      481 catagtggga aaagggtcac cggccgaggc gcctcgactt gcgcaaaggc ctccgccagg
      541 ccagtcagat accgttccag ggtaaagctg cggagaaaga taccccgcaa ctcttccccg
      601 atctcaaccg tcatcaacgc gagcagcgcg tcggcaatgg gccccgttga ttggtcggcc
      661 acgaggccgg gataaccggg cgggaacatc tccggtatgg aacgccagcg tgtggtcaca
      721 atcggcaggc caaaagccat cgcctcgatc agtgtcaccg gctgattctc agccgggtaa
      781 cgggtgggaa aacaatacag atcagcttcg cgcaaggccc tttccttttc caagccggag
      841 acaaatccca ggcaagccac ggcgccggca aactcggggt tcttcagccg ctcgtcgaat
      901 tccagtttct cctcgtcggt cacgaaagtg ccggcagcga acagcttcat ttggacaggg
      961 catttccgcc tgcttagaat ctggttggcc agaaccactg cctgcgcggc ggcaaacagc
     1021 cccttttcac gcataaagtg agcgaggtat aaaaccctca cgatctgcgg gtcgccgccg
     1081 gtgccttcga gatcggtcgg gccgagcggc tcgttccgaa acaatttggc ccgcgcggca
     1141 aagcgcgctt tccgccgtgg caataccgtc ttctcaaaat cgcagcatgg atcaggaatg
     1201 ccgtaattga cccggcaaat gcgctgggaa aagagtttct cggcgtcagc gacattgtat
     1261 ttggaaataa ccacgctcag cgccggccgc ttgaaagcgt gatacgtcac cgcgcgggtg
     1321 cgtatttgga ctgaggtttc cagccacttg gccagaccgg cggcgtgcca gtgcaggatg
     1381 attcgtttga aaaaaggacg gcagatgaac attaccatcc agtcgcggta aagggcggcg
     1441 cgtttgcccg gcgcgggaac gtaataaaaa ctgtccacgc cgtaacggaa ccgacaccag
     1501 atggcttcca ggcagaacag gaaaatccgc aggagcttga tgctctggat ttcgccgatg
     1561 tcttcgaggg tcctggacag ccgggcgttg acgtgatagc actcgatgcc gaagcgcgcc
     1621 tccgggccgc cgtccgccgc ccgccggacc cgctgccggc ggtctccgcc gaaacccgcc
     1681 aacatgagct gcaccatgta gctctggcca tggtgaggcg gcggtgtgtg agcgaatacc
     1741 agcagtttca tgcagctcag gacaggtgcc cagcgattat gtctctcagc aagttaacag
     1801 ccgaacctag ctaaaaacgc cgcgcctgag aagttttttc ttgaataccc gcacctcccc
     1861 ttgaactctc ggcaccaatg actgcgctcg aactcctggg caccccgctg ctgttgaccg
     1921 actatcgcgg gctggacgag ctatgccgcc aatgggctcg cgccggtgcc tgcgtcgccc
     1981 tggacttcgc caatacccag atcgttacca tgcgccgcca tgagccctgg ttccgtgacc
     2041 tgaccagcgc ctatgattgc ttcccgccag acggcatgcc gctgatctgg tgcctgcgcc
     2101 tggccggggc gcgcctccgc gaccgcgtct atggcccaac cttcatgcgt caattcctga
     2161 tcggagcgcc cgccgaggcc acccattacc tgctcggcgg ctcggcagcc tgcggggccc
     2221 agctccgcgc cgtcttcggc aagctcaacc ccggcctgcg cttcgtcggc gcctttcacg
     2281 gtaagtgcga ggccgatggc cggatggagg gggctgccga caaggcggtc atcgaggaga
     2341 tcaaccgcct atcccccgac ttcatctggg tcggctttgg cacgcccaag cagcaagcct
     2401 gggtaaagcg ctacaagtcc cggctgcggc gtggagtgat actgaccgtc ggcttcgcct
     2461 tcgatgtaaa cgccgggatg aaacccgacg ccccgccctg gatgcagcgc ctgggcttga
     2521 cgtgggtgta ccggctggcc tccgaacccc gccggctcgc ctcgcgttac atccgctaca
     2581 acaccctgtt cctcttctac ctgctgtggg atggcctgcg cggccgtgcc tggaccagaa
     2641 gttccggttc cacgccacgc tagccagaac cggcagaggt tgccggagtc gttttgattg
     2701 cggattcacg cttccccgcc taagttgcgg gcgtgaagct gtcagtgaag agcgattatg
     2761 ccgcccgggc ggtgctgggg ttggcgcgtc attatccgag ccaggtcgcg ctgacaacgg
     2821 atcaaatggc ggaagagcaa gggctgccgc cgaagtatct cccgcagatt ctcctcgagt
     2881 tgaaggccaa agacatcgtg cgcagcgtgc gcggaaaggc cggcggctac ttgctgacgc
     2941 gcccgccggc ggagatcaca ctgggggacg tgctgcaggc ggtggatggg ccgctattcg
     3001 acgcgccggg gctaagcagt tcgggcggtc cgccggaact gcggcgcgcc tggcagaaac
     3061 tgcggcaagc ggtggaaaac gccgtcgggg ccatcacctt ccagcagttg cttgcggaag
     3121 ctgccgagaa ggaaaagatg tactacatct gagcgccgca aacggctcag gagttccgca
     3181 cgaacagctt ccccgacaac tggcggatca ggcgcttcat ctccaggttt agcagcgcaa
     3241 cggatacggc ggaactgggc aggccgctgt ggcggatcac agcgtcaatg ccgacctctt
     3301 cgttgcccaa gcggtcatag accttctgct cgttctcgct caactccagc gcgggcagta
     3361 cgcccgtctc gccggcggac ggcgggcggt tgctggacgg gaacaggtac tcgaattcgc
     3421 tcagaatgtc ttccatgcct tcgcaaagct tg
//
LOCUS       DATCGS010000068         4095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_154814, whole genome shotgun sequence.
ACCESSION   DATCGS010000068 DATCGS010000000
VERSION     DATCGS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154814"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VIL39_01350"
     CDS             <1..934
                     /locus_tag="VIL39_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5480104.1"
                     /translation="CDYINKPFDVSTMRAAVANAMHRRTLESEIHTKADKLQQLLGEL
                     QSHKIEEQMANTRGEIYASIIHDINGPLTVISGFIQLMNQRIGNATKLEVEDLEFIKD
                     RLKTITRQVTNCIEISRRYLGFLRRQSEDAPRVGVNQLLKDLGHLVRVHPSLQSNQFT
                     VHPLAQDISVSMNGTDVIQVLLNLTVNALQCTPQPHSVEIEGRVLHQPLDLVAFKDGP
                     QDRLLNVENFENGVPLLMVSVCDSGPGIPPEILPKIFQAYFSTKGAHQGTGLGLSIVQ
                     RLVKEARGALHVHTQVGQGTTFTLYLPAIPLVVA"
     gene            1106..1228
                     /locus_tag="VIL39_01355"
     CDS             1106..1228
                     /locus_tag="VIL39_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480105.1"
                     /translation="MGDKSPKSVHKQVAQKQVKANVAAQKKQQAAAVKQVAGKK"
     gene            1415..1558
                     /locus_tag="VIL39_01360"
     CDS             1415..1558
                     /locus_tag="VIL39_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480106.1"
                     /translation="MTPDRLAFALREDSRIGFEPSCHYFYLPLDLVEKVVNCRWLLAQ
                     FKE"
     gene            complement(1578..3332)
                     /locus_tag="VIL39_01365"
     CDS             complement(1578..3332)
                     /locus_tag="VIL39_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480107.1"
                     /translation="MTKHYPLALLAIALSAMLAEGTPPGLERAEPSPLSTFFVAPTQV
                     LWQSDAGVKNAGSLLKPHAGQAVLKEPQPPCVLAASSNSPAAVLVDFGKELHGYVELI
                     TPMTSEQTKLRRIRLRFGESASEAMAELGGKLNAQNDHAIRDQVVTLPWLGKKMAGPS
                     GFRFVRIDNLDPQLPVELSQVRAVLQIRDIPQVGSFKCDDERLNRIWQTGAYTVHLNM
                     QEYLWDGIKRDRLVWIGDMGPEVSTINAVFGYNDVVPRSLDLTRDVTPVTEWMNGISS
                     YSMWWVLIHEDLWMHRGNREYLEAQKGYLTPLLTRLANLIGPDGKERIDGMRFLDWPS
                     SPNTQGVTAGLQGLLVMTLESGARLMSALGDADTAKLCTEAATRGRKVVPDVNKSKSG
                     AALLSLAGMMDAKQVSDTVLKDGGPKNISTFYGFYVLQALAKSGDADTALDFIRTYWG
                     AMLDLGATTFWEDFNLDWTSNAARIDELVPPGKKDIHGDYGAYCYQGFRHSLCHGWAS
                     GPTAWLSQNVLGVKPLEPGFKRVRIAPQLGNLKWAEGSYPTPFGPIRVRHDRQPDGSI
                     KSKIDAPPGIQVESPAKP"
BASE COUNT          895 a         1208 c         1214 g          778 t
ORIGIN      
        1 gtgcgactac attaacaagc cgttcgacgt ttccaccatg cgcgctgctg tcgccaacgc
       61 catgcatcgg cgcactctcg agagcgagat ccatacaaaa gccgacaagc tgcagcaact
      121 gctgggcgag ctccagagcc acaagattga agagcagatg gcgaacaccc gcggggaaat
      181 ctacgccagc atcattcacg acatcaatgg cccgctcacg gttatctccg gcttcattca
      241 gctcatgaac cagcgcattg gcaatgcgac gaagctggag gtagaagacc tggagttcat
      301 caaggaccgg ttgaagacca tcacccgcca ggtgacgaac tgcatcgaga tctcccgccg
      361 ttacctgggc tttctccgtc gtcagtccga agacgcgccg cgcgtgggtg tcaaccaact
      421 gctcaaggac ctcggccacc tggtccgggt gcacccgagc ctgcagagca accagttcac
      481 tgtccacccc ctggcgcagg acatctctgt gagcatgaac gggaccgatg tcatccaggt
      541 gctgctcaac ctgacggtca atgctttgca gtgcacgccg cagccgcatt cggttgaaat
      601 cgaaggccgc gtgttacacc aacctctcga cttggtggca ttcaaggatg gcccacagga
      661 tcgcttgctg aacgtggaga actttgaaaa cggtgtgcca cttctgatgg tttccgtctg
      721 tgatagcggg cccgggattc cgccggaaat cttgccgaag atcttccagg cctatttttc
      781 tactaagggc gcgcatcagg gcaccggctt gggcttgagt atagtccagc gcctcgtcaa
      841 ggaggcccgg ggcgcgttgc acgtccacac ccaagtcggg caggggacga cttttacgct
      901 atatcttccg gccatcccgc tggtcgtcgc ttagccgtta ttccgtgtga acgggacggc
      961 gtctcaatcc ccaacgcgga actcctttgg gttgaggtcg tgtacggtgc ttgcagcgtt
     1021 cgcaaatgtc ggctactgtt accaatatcc ggcaaccgac taaacacagc cgggcaacaa
     1081 accgtcaaag aaaggcaaag tgaatatggg cgacaagtct ccgaagtccg tacataagca
     1141 ggtagcacag aagcaagtta aagccaacgt ggctgcccag aagaagcagc aagccgccgc
     1201 cgtgaagcag gttgcgggta agaaatagcg ggggccactg agagtttcga cccggcacat
     1261 tgccggtcct gagcggagct gctggcgacc gtgcgacctg gtttccattg gtttgcgcgg
     1321 ccttggattg cgaatatggc cagttgggct cgggcggttg gggcatggcg cagagaaaac
     1381 tcgcgcagta gcggcattgc tgacgctgct ggaaatgacg ccggaccgac tggctttcgc
     1441 gctgcgggag gattcgcgga tcggttttga accctcgtgc cactacttct atttgccact
     1501 cgacctagtg gagaaggtgg tgaactgccg ctggttgttg gcacaattca aggaatagtg
     1561 cgcaagacaa taggccttca gggcttagct ggcgactcga cttgaattcc cggcggcgcg
     1621 tctattttcg atttaatgga gccgtccggc tgccgatcat ggcggacgcg gatcgggcca
     1681 aacggcgttg ggtaagagcc ctcggcccat ttcagattgc ccagttgcgg ggcgatgcgg
     1741 actcgcttga agccgggctc aagcggtttc acgccgagca cattctggct cagccacgcc
     1801 gtcgggccgc tcgcccagcc gtggcagagg ctgtgccgga aaccctgata acaataggcg
     1861 ccgtagtcgc cgtgaatatc ctttttgccc ggcggcacga gttcatcaat gcgcgcggcg
     1921 ttggatgtcc aatccaggtt gaaatcctcc cagaacgtgg ttgcgccgag gtcgagcatc
     1981 gcgccccaat aggtgcggat gaaatccagc gccgtgtcgg cgtccccgga tttcgccagc
     2041 gcctgcaaaa cgtagaagcc atagaaggtc gagatgtttt taggtccgcc atccttgagc
     2101 acggtgtcgg aaacttgttt ggcgtccatc atcccggcca gcgagaggag cgcggcgccc
     2161 gatttggact tgttcacgtc gggcacgacc ttgcgtccgc gcgtcgccgc ttccgtgcaa
     2221 agtttcgcgg tgtcggcgtc gccaagggca ctcatcaagc gagccccgct ctcgagggtc
     2281 atgacgagca atccttgcaa accagcggtg acgccctgtg tgttcggcga actgggccaa
     2341 tccaggaatc gcatgccgtc gatgcgctcc ttgccgtccg gcccaataag attcgccagg
     2401 cgcgtgagca gcggcgtgag atagcccttc tgcgcttcga gatattcccg gttgccgcgg
     2461 tgcatccaca agtcttcgtg gatgagcacc caccacatcg agtagctgga gatgccgttc
     2521 atccattcgg tgacgggcgt gacgtcgcga gtgaggtcca gcgagcgcgg cacgacgtcg
     2581 ttgtagccga acacggcgtt gatggtgctc acttccgggc ccatgtcgcc gatccacacc
     2641 agccggtcgc gcttgatgcc gtcccagagg tattcctgca tgttgaggtg aacggtgtag
     2701 gcgccggtct gccatatccg attgagccgc tcgtcatcgc acttgaaaga accgacctgg
     2761 ggaatgtcgc ggatctgaag caccgcgcgc acctggctca gttccacggg caattgcgga
     2821 tccaggttgt ctatgcgcac aaagcgaaaa ccgctcgggc cagccatctt cttgccgagc
     2881 cagggcagcg tcaccacctg gtcgcgaatg gcatggtcgt tttgggcgtt cagtttgccg
     2941 ccgagctccg ccattgcctc gctggcggac tcgccgaaac gcaggcggat gcgacgcagc
     3001 ttcgtctgct cggacgtcat tggcgtgatc aattcgacat agccatggag ttccttgcca
     3061 aaatccacca acaccgcggc gggcgagttg gaggaagcgg ccagcacgca ggggggctgc
     3121 ggttccttga gcacggcctg cccggcatgg ggtttgagca gcgaaccggc gtttttcacg
     3181 ccggcatcgc tttgccacaa gacctgcgtg ggcgcgacga agaaggtcga gagcggggac
     3241 ggctccgcac gctccaatcc cggcggcgtg ccctctgcga gcatcgcgga aagggcaata
     3301 gcgagtaagg ccagtggata gtgctttgtc attcttcttt cctataatgc tttgccgcct
     3361 gtaatacgca aggtgcggaa ctggctgggg acggcacagg cggctcgccg aaactcagat
     3421 tgtccgcagg ctccgtggag aaccagcgcc aacagacctt cgacaaacag cgtgaatgcg
     3481 cgactcataa cgctgaaatg attgtcgcgc agatccgcca gagtcgagtg gaaactgcca
     3541 gaccgatctg acgcagcggc ggatggagcg agctggataa gtgtggctga tcgaaaacgg
     3601 ggggttgaag ggattctatg ggtataggac ctgaacatcc aacatgcaac atcgaacaac
     3661 gaacatgcat tgcctgcccg aagcacgctg attggatgtt ccgtatgaag aagagcagcc
     3721 caaagccacc caaagccaca attgggcaaa agctgaaatg ctgaaagctg aaagctgaaa
     3781 gctgaaatca gaaagagggc tgcacaaagc cacccaatgc gacatcaaag ccaccccaag
     3841 gcgtattgat agccaactga taggcacccc aaagccaccc taaagccacc ccaaggctac
     3901 cccaaggcta ccccaaggct accccaaggc taccccaagg ccaagggaat gagaggcccc
     3961 caaatcctcg cctaagcgca tcgcgacgaa aaaagcgcgg gagagcccgc ccccagggct
     4021 ttcgttttcc gcgcgactgc tcaaaatcca cgccctaacc gccaaaaaag atcacaacct
     4081 actcatttag agaca
//
LOCUS       DATCGS010000069         5503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_105592, whole genome shotgun sequence.
ACCESSION   DATCGS010000069 DATCGS010000000
VERSION     DATCGS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105592"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VIL39_01370"
     CDS             <1..794
                     /locus_tag="VIL39_01370"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5480108.1"
                     /translation="TQLPKVKQSMNTTSTLRAIIISVALLAVLAFTLPGTAAEQPGKI
                     KVLLVTGDDAQPAHNWREVSQALRETLISSGRFDVRICEDAGVLDSGATLGRYDLVFL
                     HMYNAKTPTLSAGAKENLASFVKDGKGLVISHLSSASFKEWEEFPKLCGRYWVMGKSG
                     HGPRAMFKARVTKSDHPITQGLADFEADDELYSKLQGDAPITVLVEADSDWSKKTEPL
                     AFTVEYGKGRVFHETFGHDAKALSSPTVQKLIQRGSEWAATGKVQ"
     gene            1010..1561
                     /locus_tag="VIL39_01375"
     CDS             1010..1561
                     /locus_tag="VIL39_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480109.1"
                     /translation="MNVAEPAGWCENLYRAKASGLILYGRALGLSHGEAEDVLQETFV
                     ALMQRPEPPELPVHYCVRSYRNRALNYRRSLWRRLTRELESVRWFERSAAENSSERAA
                     MRGLAALPPEQREVIVLKIWHQHTFEEIGELLEISPNTAAGRYRYGLQKLRTFLKGPL
                     YERDERVGEPIALVDAAPPLGEA"
     gene            2048..2623
                     /gene="grpE"
                     /locus_tag="VIL39_01380"
     CDS             2048..2623
                     /gene="grpE"
                     /locus_tag="VIL39_01380"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY5480110.1"
                     /translation="MKKSNAHIEKQSAASAPPATPEPLAPVEPAPLTPEQLRELNDRA
                     VKADENWERLLRTTADFDNFKKRAAREKQEAIKYANESLLEKLVPVLEHLDMALAAAQ
                     AAGPEAGQSLQAGVSMICQQFRHVLAEAGLEELDALGKLFDPHVHEAISQQETLDVPE
                     GQVVQQLRKGYKFRDRLLRPASVVVAKQPVA"
     gene            2627..3751
                     /gene="dnaJ"
                     /locus_tag="VIL39_01385"
     CDS             2627..3751
                     /gene="dnaJ"
                     /locus_tag="VIL39_01385"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5480111.1"
                     /translation="MAKRDCYEVLGIGRNAEPDEIKKAYRKLALKYHPDKNPGDKAAE
                     ESFKELGEAYEILSEPDKRALYDQHGHAAFDRRAGGYGGGAFHDPFDIFRDVFGGGGI
                     FEDLFGGGRSDPSQPQRGNDLRYDMEITFEESAYGCEKEVTVNKPAPCETCQGSGAEA
                     GSRVRTCPSCAGRGQVVNSRGIFSIAQTCPHCQGAGRILEKPCKACRGEGRRERSSQI
                     KLRIPAGVDTGSRLRSAGNGEAGFRGGPPGDLYVVLHVQAHAIFQRDGDDLLCEVPVS
                     FVQAALGAEIEVPALDGRTPINIPPGTQPGTMFRLKSKGIKNVQGYGRGDLHVRINVE
                     VPTHLSAAQRAKLQEFASLCNGKENPMSQSFLDKAKKLFH"
     gene            3872..4621
                     /locus_tag="VIL39_01390"
     CDS             3872..4621
                     /locus_tag="VIL39_01390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY5480112.1"
                     /translation="MHRFYLPPEQCRDASLLLTSREAHHALHVVRVRRGEHVTVVDGA
                     GCELLCEVQECDRDKVRLAVVEKRCQPAPPSQVTLLQAVPKGKAMEVIIQKATELGTF
                     RIVPLLSERVVTHLHDQDAAHKAGKWRSVAVEAIKQCGSAWLPELEAPLTLDQFFARK
                     ESFELQLIASLASGSRPARECFRAFHAQHGRPPRSVCVWIGPEGDFTPVETGAIESHG
                     ALPITLGRLVLRAETAAIYCLSILNYELQSP"
     gene            complement(4618..>5503)
                     /locus_tag="VIL39_01395"
     CDS             complement(4618..>5503)
                     /locus_tag="VIL39_01395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5480113.1"
                     /translation="RDILDWLHKHRPELFGALEYWIVEPSGGRRNWQQRKLREYGNRV
                     HWVGELAGATGRAFPDSRVQHSAGLRGICFSNELLDAMPVHRLGWDAKARVWFECGVT
                     LQAGQLVWTQMPGGAPDNRNHQSSARDLISQFQLPIDHGLLDTLPDGFTTELCPAAEE
                     WWRTAAMALGCGKVLTFDYGLSAEEFFMPERKEGTTRGYHHHRPSSNVLAHPGEQDIT
                     AHVNFTAIRTAGESAGLRTDAFLTQAQFLTSIAARIWKDEGSFGEWTPERTRQFQTLT
                     HPEHLGQSFRVLVQGRRI"
BASE COUNT         1131 a         1687 c         1640 g         1045 t
ORIGIN      
        1 taactcaact gccgaaagtg aaacaatcta tgaacacgac ctcaactctt agagcaatca
       61 taatcagcgt ggcccttctg gctgtattgg ctttcaccct gcctggcacg gcggccgagc
      121 aacccggcaa aatcaaggtg ctcctggtga cgggcgacga tgcgcagccc gcccacaact
      181 ggcgcgaggt ttcccaggcc ctcagggaaa cgctcatctc gagcggcaga ttcgacgtga
      241 ggatctgcga ggacgccggc gtgctcgatt ccggcgcgac cctgggccgc tacgacctcg
      301 tcttcctcca catgtacaat gccaagacgc cgacgctctc cgccggcgca aaggagaatc
      361 tcgcaagctt cgtcaaggac ggcaagggcc tggttatttc gcacctgtcc agcgcctcct
      421 tcaaggagtg ggaagagttt cctaaactct gcggccgcta ctgggtgatg ggcaaatccg
      481 gtcacgggcc gcgcgcgatg ttcaaggccc gggttacgaa aagcgatcac ccgatcacgc
      541 agggcctggc cgacttcgag gccgatgacg aactctattc caaactgcag ggcgacgcgc
      601 cgattaccgt actggtggaa gcggattccg actggagtaa aaagactgag ccgctcgctt
      661 ttacggtcga atacgggaag ggacgggtgt tccatgaaac gttcggccac gacgcgaagg
      721 cccttagcag ccctacagtg cagaaactga tccagcgcgg cagcgaatgg gccgcgacgg
      781 gcaaggtcca ataaggctag caacggcacg gcggcacgct gggagtgatc tgtcggggag
      841 gcgggcttat gttccaagga ccaggtcgcg gaacctggtc ctcttcttgt tctcacacct
      901 ttgtagcctg caatttcaga tttccgcatg gttgcgtgga cgcgcaagtt ggatttcctg
      961 caaaagttta tacagattcc cgcttccaca tgtctattag ggtgaacttg tgaacgttgc
     1021 tgagccagcc ggctggtgcg agaatctgta tcgcgcgaag gcgtccggac ttatcctgta
     1081 cggacgggcg ctgggcttgt ctcatggcga agccgaagac gtcttgcagg aaacctttgt
     1141 tgcgctaatg caacgcccgg agcctcccga gctgccggtg cactattgcg tgcgcagcta
     1201 tcgcaaccgg gctttgaatt accgccggag cctctggcgg cggctgacgc gcgagcttga
     1261 atccgtgcgc tggttcgagc gctccgccgc ggagaactcg tcggagcgcg cggcgatgcg
     1321 cggtttggcc gcgctgccgc cggagcagcg cgaggtgatc gtgttgaaga tctggcatca
     1381 acataccttt gaagaaattg gcgagttgct ggagatttcg ccaaacaccg cggcgggccg
     1441 ttaccgttac gggctgcaga aactgagaac gttcctgaaa ggaccattgt atgaacgaga
     1501 tgaacgagtt ggagaaccaa ttgctcttgt ggacgccgcg ccgcccctcg gcgaggcttg
     1561 aacggcagct ttttgcctcc gggccggtct cggcggaagt gctgccccta ttgcgtctca
     1621 gttggctggc accggctacg gcggccttga tgctgatgtg cgtgctgttt aatcaatgct
     1681 acgggcccgc tctctcggtc tcggccccct cgggtcccct ggtcgccatg atccttagca
     1741 accagagtgc ggccgcgtac ctgcctggca gttttcgggc cgaacgtaac aacctgcccg
     1801 ctgacgtctt taactggaca agccgcagcg gttcgactcc tggaggcgct tcccgttggc
     1861 agtcgtatgg gagcaagtcg ccctgagcga cgcaaacgga agaggggcgg ctgccaacgc
     1921 gccgccagtc tcgcccctgc cgtccgatcc aggctccgtt taaccgactc cgtttagcgg
     1981 gttgccgtgc gctctcctat ggtctattct cagagcgttg gaattgtgga tctcgaaacg
     2041 gtatcccatg aagaaaagca acgcacatat tgaaaagcaa tcggcagcca gcgccccacc
     2101 ggccaccccg gaaccgctgg cgcccgtgga gccagcgccg ctgacccccg aacaattgag
     2161 ggaactcaac gatcgagcgg tcaaggccga cgagaactgg gaacgcttgc tgcgcaccac
     2221 tgcggatttc gacaacttca agaagcgcgc agcccgtgag aagcaggaag cgattaagta
     2281 tgccaacgag agcctgctgg aaaagctggt gccggtgctg gaacatctgg atatggcgct
     2341 ggcggcggct caagctgccg ggcccgaggc tgggcagtcg ctgcaagcgg gagtcagcat
     2401 gatctgccaa caattcagac atgtgctcgc cgaagccggc ctcgaagaat tggacgcgct
     2461 cgggaagctt ttcgacccgc acgtgcatga agccatttcg caacaggaaa cgctggacgt
     2521 gcccgaaggt caggtggtcc agcagcttcg gaaaggctat aagttccgtg atcggctctt
     2581 gcgcccggcc agcgtggtag tcgccaagca acccgtcgcc tgagccatgg ctaaacgcga
     2641 ttgttacgaa gttctcggca tcgggcgaaa tgcggaaccg gacgaaatca agaaggccta
     2701 ccgcaagctc gcgctgaagt atcatccgga taagaatccc ggcgataagg cggccgaaga
     2761 aagcttcaag gaactcggtg aggcttacga aattctgagc gaaccggaca agcgcgctct
     2821 gtacgaccag cacggccacg ccgcctttga ccgccgtgcc ggcggctatg gaggcggagc
     2881 cttccacgat ccgttcgaca tcttccgcga cgtcttcggc ggcggcggca tttttgaaga
     2941 cctcttcggg ggggggcggt ccgacccaag ccagccacag cggggcaacg atctccgcta
     3001 cgacatggaa attaccttcg aggagtccgc ctacggctgc gaaaaggaag tcacggtcaa
     3061 caagccagcg ccttgcgaga cttgccaggg atcgggcgcg gaagccggtt cccgcgtccg
     3121 gacctgtccg agctgcgccg gccgcggtca ggttgttaac tcgcgtggta ttttcagcat
     3181 cgcccaaacc tgcccgcatt gccaaggcgc gggtcgcatc ctggagaaac cctgcaaagc
     3241 ctgtcgcggt gagggacgcc gcgaacggtc ttcgcaaatc aaactgcgca tccccgccgg
     3301 tgtggacaca ggctcgcgcc tgcgctctgc cggcaatggt gaagcgggct tccgtggcgg
     3361 accgcccggc gatctgtacg tggtgctcca cgtgcaggcc catgccatct tccagcgtga
     3421 cggcgacgat ttgctttgcg aggtgccggt gagtttcgtg caagctgcgc tcggcgcgga
     3481 aatcgaggtc ccagcgctgg acgggagaac tcccattaac attccaccag gcacccagcc
     3541 tggcacgatg ttccgcctga agagcaaagg catcaaaaat gttcaaggct atggccgcgg
     3601 cgatctgcac gtccgcatca atgtcgaggt gccgacccac ctgtcggctg cccaaagagc
     3661 caagctccag gaattcgcca gtctgtgcaa tggtaaggaa aaccccatga gtcaaagctt
     3721 cctcgacaag gcaaaaaagc tgttccattg aacgcaaccg ggtgacggaa taatggagta
     3781 ttgaagtgtc cggatgccgc caataccatc cggagcagag ctttcattcc ccaagactcc
     3841 aatactgcaa ctgaccatca ctccacttcc catgcatcgt ttctatctgc cgccggagca
     3901 atgccgagac gcaagcctct tactgaccag ccgcgaagca caccatgctc tgcacgtcgt
     3961 gcgcgtccgc cgaggtgagc acgtcacggt ggtggatggc gcgggctgcg agcttctgtg
     4021 tgaagtacag gaatgcgacc gcgacaaagt caggctggcc gtcgttgaaa agcgctgcca
     4081 gccggccccg cccagccagg tcacgttgct gcaagccgtg cccaagggca aagccatgga
     4141 agtgatcatc cagaaggcaa ccgagttggg gacttttcgc attgtgcctt tgctctccga
     4201 acgagtcgtg acccacctcc atgatcagga cgccgcgcac aaggccggga aatggcggtc
     4261 ggtcgcggtc gaggccatca aacagtgcgg ctctgcctgg ctgccggaac tcgaggcgcc
     4321 gctgaccctg gaccaattct tcgcgcggaa ggagagtttc gagctgcagt taatcgcctc
     4381 gctcgcaagc ggcagccgcc cggcgcgaga gtgtttccgc gcgtttcacg cacagcatgg
     4441 ccgaccaccg agatcggttt gcgtctggat cggccccgaa ggtgatttca cgccggtcga
     4501 aacgggggcg atagagtctc acggcgccct gccgattacc cttggccgac tcgtcttgcg
     4561 tgccgaaacc gcggccatct attgcctctc aattcttaac tacgagttgc agtcccctta
     4621 gatcctgcga ccctgcacca gcactcgaaa tgactgtccc aaatgctccg ggtgggtcag
     4681 ggtttgaaac tggcgcgtgc gctccggagt ccattccccg aacgagcctt catccttcca
     4741 gatccgcgca gcaatgctgg tgaggaattg cgcttgagtc aggaaggcgt ccgtcctcaa
     4801 gccggctgat tccccagcgg ttcgaatggc cgtgaaattc acatgtgctg tgatgtcctg
     4861 ctcgccggga tgggcgagga cgttgctgct gggccgatga tggtgatacc cccgtgtggt
     4921 gccttccttt ctttccggca taaagaattc ttctgccgat aggccataat caaacgtgag
     4981 cactttgccg caccccaagg ccatcgctgc cgtgcgccac cactcctcgg ccgccggaca
     5041 cagctcggtc gtgaaaccgt cgggcaacgt atccagtaat ccgtggtcaa tgggcaattg
     5101 gaattgggaa atcaagtcgc gagcgcttga ttggtgattt ctgttatccg gggccccgcc
     5161 aggcatttgg gtccagacga gttgcccagc ctgcaacgtc acaccgcatt cgaaccagac
     5221 ccgcgctttc gcgtcccatc ccagccggtg cacgggcatg gcatcgagca actcgttgga
     5281 aaaacaaatc ccccgcaaac cagccgagtg ctgaacgcgg gagtcaggga atgccctgcc
     5341 ggtcgccccc gccaactccc ccacccaatg gaccctattg ccatactcac gcagcttccg
     5401 ctgctgccag tttcggcgcc cccccgaggg ttccacaatc caatactcca acgcgccgaa
     5461 tagttccggg cgatgtttat gcaaccagtc caaaatatcc ctg
//
LOCUS       DATCGS010000070         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_253428, whole genome shotgun sequence.
ACCESSION   DATCGS010000070 DATCGS010000000
VERSION     DATCGS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253428"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="VIL39_01400"
     CDS             complement(<1..1800)
                     /locus_tag="VIL39_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY5480114.1"
                     /translation="MKRFRLPLAAAAALALALHGTAASETNLYIRVNQVGYRPADTKI
                     AMAFSTSAIGSSFAIYQAGPASSESKAAYEGKVAVIEGGTWGKWTQHADLDFSTFRKP
                     GRFVLRIGDASSPVFAIDDKVYEPLPDQLLEFMREQQCGYNPWLGKPCHTNDGRTAFG
                     PLPYGTDLDCTGGWHDAADLLKYLITSANATAQLLLASELQDVTTNHRAGYRSMFKDI
                     ATARGDAGSNGIPDVLDEGRWGLEWMLKLHPAPDALYHQVADDRDHCGPRLPYAEIAD
                     YGWGRGSNRVVYFADGQPQGLGKYKSTATGVANLAGRYAAAMALGQIAFKKIDPAFAQ
                     RCLKAGIEVYALGRAKEGAQQGNSYKEPYRYEETTWADDMEWGAAALYRVTGEKHYLE
                     EAKRYAKLAASESWMGQEQTKHYQYYPFMNTGHFMLYDLVDRETQRLLAQYYRDGIER
                     CIRMGQKNPYRAGVPFIWCSNNLIVALVTQCLLYERMTGDPQYHAFMARQRDWLLGRN
                     PWGTSMFTDIPAGGVYPHQPHLTTTWLTHRSVRGGLVDGPIYERIFKTQLGVHVQQPD
                     PFAEFQSELAVYHDDHGDYVSNEPTMDGTASAIL"
     gene            complement(2012..>2271)
                     /locus_tag="VIL39_01405"
     CDS             complement(2012..>2271)
                     /locus_tag="VIL39_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY5480115.1"
                     /translation="IPIVRTTGGLDDTVIDVREDAEKANGIKFIEYSATALAKSIRKA
                     LAIFEQPALLKHFRMNAMAADFSWNRTVAQYVQAYQRARPK"
BASE COUNT          447 a          674 c          679 g          471 t
ORIGIN      
        1 gaggatcgcc gaggccgttc catccatggt cggttcgttg gaaacgtaat ctccgtggtc
       61 atcgtgatag acagccagct cggactggaa ttcggcaaag ggatcgggct gctgaacgtg
      121 tacccccagt tgggtcttga agatgcgctc ataaatcggc ccatccacca gtccgccccg
      181 gaccgaccgg tgtgtcagcc aggtggtggt cagatgcggc tgatgcggat agacgccgcc
      241 ggccggaatg tccgtaaaca tagaagtgcc ccagggattg cgccccaata gccaatcgcg
      301 ttgccgggcc atgaaggcgt gatactgcgg gtcgccggtc atgcgctcgt agagcaggca
      361 ctgcgtcacc agcgccacga tcaggttgtt cgaacaccaa ataaacggca cccccgcgcg
      421 gtagggattc ttctgtccca tgcggatgca gcgctcaatg ccatcacggt aatactgggc
      481 gaggaggcgc tgagtctcac ggtccaccag gtcgtagagc atgaaatgac cggtgttcat
      541 gaagggatag tactggtaat gcttggtttg ctcctggccc atccaggatt ccgacgcggc
      601 cagcttggcg taacgcttcg cctcctcgag gtaatgcttc tcgccggtca cccggtagag
      661 agcggcggcg ccccactcca tgtcgtcggc ccaggtggtt tcctcgtaac gatagggctc
      721 cttgtaggag ttgccttgct gcgcgccttc cttggcccgg cccagcgcat acacctcgat
      781 gcccgccttg aggcatcttt gggcgaaggc aggatcaatc ttcttgaagg cgatctgacc
      841 cagcgccata gccgcagcgt aacggcccgc cagattcgcc acgccggtcg ctgtgctctt
      901 gtacttgcca agcccttgcg gctgcccgtc ggcaaaatac accacccggt tgctgcctcg
      961 gccccagccg taatcggcaa tctcggcata gggcaaccgc gggccgcagt ggtcccggtc
     1021 atccgccacc tggtgataga gcgcatcggg tgccggatgc agcttgagca tccattccag
     1081 gccccagcgc ccctcgtcca gcacatccgg tattccgttc gacccggcat cgccgcgggc
     1141 ggtcgcgatg tccttgaaca tactgcgata gcctgcgcgg tggttcgtag tgacgtcttg
     1201 gagttcgctg gccagcaaca gttgcgccgt ggcgttggcg gaagtgatca ggtatttgag
     1261 caagtccgcc gcgtcgtgcc agccgccggt gcaatctaga tcggtcccgt agggcagggg
     1321 accaaaggcg gtccggccgt cattggtgtg acagggcttg cccagccagg gattgtaacc
     1381 gcactgctgt tcccgcatga actcgagtag ttggtcgggc aacggctcat agactttgtc
     1441 atcaatggca aagaccggtg aactggcatc gccaatgcgc aaaacaaacc ggcccggctt
     1501 tctgaacgtc gagaaatcca gatccgcgtg ctgagtccat tttccccagg tgccgccctc
     1561 gatgaccgcc acctttccct catacgcagc tttcgattcc gaggacgccg gcccggcttg
     1621 gtaaatggca aacgacgacc cgatggcgct ggtggaaaag gccatcgcaa tcttggtgtc
     1681 tgctgggcga tagccaactt ggttgacgcg aatataaagg ttagtctcgc tagccgcagt
     1741 accgtgaagc gcgagggcca gagccgccgc cgccgccagg ggtagccgaa atcgtttcat
     1801 ctcgcggatt gtagcggcct ctgaagaaaa gaacagcgcc gacatccttc gagaagatgg
     1861 tgacggtgaa tctacgaccg ctttccggca tgacagtaca gtgccagaaa tagccggccc
     1921 accgaaaaag cggaacaact tgtacttcgc acaatgtttg ttatatttat tacatgtgcc
     1981 tagttcgcga ggagcgggct aagcccatgc gctatttcgg gcgggcgcgc tgatatgcct
     2041 ggacgtactg cgccactgtc cggttccagg agaagtcggc agccattgcg ttcatacgga
     2101 aatgctttag cagcgcgggt tgttcgaaga tcgcgagcgc cttgcgaatg ctcttggcca
     2161 gcgccgtcgc ggagtattcg atgaatttaa tgccgtttgc cttttcggca tcctctctaa
     2221 catcaattac agtatcgtcc agcccgccgg tggtgcgaac gattggaatt g
//
LOCUS       DATCGS010000071         7046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_551107, whole genome shotgun sequence.
ACCESSION   DATCGS010000071 DATCGS010000000
VERSION     DATCGS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551107"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VIL39_01410"
     CDS             <1..972
                     /locus_tag="VIL39_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480116.1"
                     /translation="DAIGEKYKIDSSRRYTGLSGYKRLLEGKLDAVVIETPPYFHPEH
                     SAAAVDAGKHVYLAKPIAVDAPGCQTVAASGQRATAKKQVFLVDFQTRADASFRDAVG
                     QVHKGTIGSLVMAEAWYPWSGGGRGAPPATPEQQLRSWYYVLPLSGDFIVEQSIHSLD
                     LVTWIINADPLRARGTGGHKVRPADSIYDHFAVTYSFPNDLVLSFTCIQSIPMVKDEI
                     RARVFGAGGFIDADYYTGVVVRGPETAVRASVGDLYNSGTVVNINEFHDAIVKGDCSN
                     PTVVPSVRSNLTAVLGREAAYRRDELTLAALLKEGKKLEPNLKGLKA"
     gene            complement(969..2882)
                     /locus_tag="VIL39_01415"
     CDS             complement(969..2882)
                     /locus_tag="VIL39_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480117.1"
                     /translation="MHFASNDSILARPSQVLLLCLVALTLTLTVPAQQPPGAGTAQTN
                     VAADVSRRVLSAPIIAPTDVGDYSGIQYPRWDAFSDTWVATDALGRSLPSCSEAGLPR
                     KHRAVGAFYFLWHYPHGDLGAFDITQILARDPQAITNAASPLWGPMHEFHHWGESIFG
                     YYLSDDEGVLRKHAQMLADAGVDVIVFDVTNQQTYPRSWKTLCRVYDQIRREGGQTPQ
                     IAFLCPFWDSKKVARELWEQLYRPGLYPDLWFRWEGKPLILADPAQTDLGPEICGRFT
                     FRKPQPDYFVGPTGPNQWAWLEVYPQHVFTNAAGAREQMTVGVAQNAVDGKLSVLSNP
                     RSLGRSFHDGREPAPADCDYSGRNFAEQWRRALQVDPEFVFITGWNEWIAMRFDHQAP
                     FHAPGPVTFVDQFNGEFSRDIEPVKGGHGDDYYYQMIANIRRYKGVRPLSPVKPQPIT
                     IDGRFGDWAAVEPEFRDTIGDPVKRDHAGWAQGQRYVNRTGRNDIIAAKVSLDATTVY
                     FYVRTREPLTPSSDPNWMLLFIDADHDARTGWLGYDFIVNRVNVHAQTTTLERNTGGY
                     RWGSPLDVPCRAAGNELELAVPRSALGLTKLPATLDFKWADNIQQTGEAADFTLNGDA
                     APNDRFNYRAIFP"
     gene            3041..4279
                     /locus_tag="VIL39_01420"
     CDS             3041..4279
                     /locus_tag="VIL39_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5480118.1"
                     /translation="MNKSQIERPAPSRFRRHWLLAPGTIFLNHGSFGACPKPVLELQS
                     ELRRQMEAEPVQFLWRRYEERLDPVRAEAATFLGAQARDLVFVTNATTGVNAVVRSLE
                     LRRGDELLTTNQDYNACHNVLVEAAHRTGAKLVTVRVPFPLASPDELVEAVLRAVTPR
                     TRLAMLDHVTSNTALVVPVARLIRELEARGVDTLVDGAHAPGMVSLNLSKLSPAYYTG
                     NLHKWVCAPKGAAFLWVRRDKQAPVQPAIISHGNNTPRPGHSAFQDRFDWAGTFDPSA
                     WLCVGEAIRWMAKLYPGGWPAIQEANHELAVRARRQLCQCLNVEPPCPEDMLGAMATL
                     PLPERFQGRPRKGKIDVEQLRLYDGFGIEVPFIRVNERRYFRISAQLYNSLAEYDYLA
                     EALLSLQPRRVYPGKGSIRR"
     gene            4367..5470
                     /locus_tag="VIL39_01425"
     CDS             4367..5470
                     /locus_tag="VIL39_01425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5480119.1"
                     /translation="MNSGRIQFVDLVAQYQQIKPEVDAAMAKVCSRGDFIMGEDVKLF
                     EQEFAAFCQVPHCVAVANGTEALKLALLACGVGPGDEVITCTHTFIATVFAIHQTGAK
                     PVLVDCDPRFYTIDTAQVERAITPRTKAIIPVHLYGQPADMDPILEVAKKHKLYVIED
                     ACQAHGAEYKGRRCGSLGDIAAFSFYPGKNLGAYGDGGAVTTKRADLAERVALLRNHG
                     QKVKYEHLVKGFNSRLDTLQAVVLRIKLRRLEQWNEARRKAAAKYDRLLAGKGLVTPK
                     VAPYAKPVYHLYVVQVPDRKKQQAAFDAANVSHVIHYPIPVHLQPAFAELGYKLGSFP
                     VTEALVPKIISLPMFAELTDSQIEQVAGACLKA"
     gene            5590..6480
                     /locus_tag="VIL39_01430"
     CDS             5590..6480
                     /locus_tag="VIL39_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5480120.1"
                     /translation="MSTETKVCVVTGAGGYVGGRLRDRLEREGWRVIAWARQPEPDSG
                     GVAFRLGQEVDSNLFKGVRALVHCAYDFGPRRWEDIAAINITGSQKLLEAARKAGVGS
                     VVFISSLSAFAGCRSLYGKAKLELESFAHTIGAAVIRPGLVYSDHPGGMFGRLVGQVR
                     GARFIPIMWGGKQLQYLVHDEDLSSLVLGCLDGRVPGRAEPITIAHDQGWELKEILRQ
                     IAQGLGKRVSFLPVPWQLVWLALKSLEVAGVRTNFRSDSLISMIYQNPRPSFAWLKPL
                     GFQCRPFHWAPTSSGEQATQ"
     gene            complement(6662..>7046)
                     /locus_tag="VIL39_01435"
     CDS             complement(6662..>7046)
                     /locus_tag="VIL39_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480121.1"
                     /translation="DAATATLWLNPASESDRGATAADLTNAVSISSFNFRQDSGCGAT
                     MLVDDLKVGLSFAAVTGTNAVLNRISLVVKHSADNLILTWTNSAFGLQAASTVTGAYA
                     PVPGATSPYTNAIGAFPRFFRLRAN"
BASE COUNT         1329 a         2017 c         2365 g         1335 t
ORIGIN      
        1 gacgccatag gagagaaata caagatcgac tcgtcgcgcc gttacacggg cctgtccggc
       61 tacaaacggt tgctggaagg gaagctcgac gccgtggtca tcgagacgcc gccctacttc
      121 cacccggagc attccgccgc tgccgtggac gcgggcaagc atgtctatct ggccaaaccg
      181 attgccgtgg atgcgcccgg ctgccagacg gtggccgcgt cgggccagcg cgccaccgcg
      241 aagaagcagg tgttcctggt tgatttccag acccgcgctg atgccagttt ccgcgatgcg
      301 gttgggcagg tccacaaggg caccatcgga tcgctggtca tggccgaggc ttggtatccg
      361 tggtcgggtg gtggccgagg tgcaccgccg gccacgcctg agcagcaact gcgcagttgg
      421 tattacgtgc tgccgctctc cggcgatttc attgtcgagc aaagcattca ttccctggat
      481 ctagttacct ggatcatcaa cgccgatccg ttgcgcgccc gcggcaccgg cggccacaag
      541 gtgcgcccgg cagacagcat ctacgatcac tttgcggtca cctactcctt cccgaacgac
      601 ctggtgctgt catttacctg tatccagagc attccgatgg tgaaggatga aatccgggcc
      661 cgggtgtttg gggccggggg ttttattgat gcggattatt acaccggtgt ggtggtgcgc
      721 ggcccggaaa ccgcggtgcg ggcgagcgtc ggcgacctct ataacagcgg gaccgtggtc
      781 aatatcaacg agttccacga tgccattgtc aaaggagact gctccaaccc gaccgttgtg
      841 ccatccgtgc ggtcgaacct gactgccgtc ctgggccgcg aggccgcgta tcgccgggat
      901 gagctgacgc tggcggcatt gctgaaggaa ggcaagaagc tggagccaaa tctcaagggg
      961 ctgaaggctt aggggaagat cgcgcggtag ttaaagcggt cgttgggcgc ggcgtcgccg
     1021 ttgagtgtga aatcggcggc ctcgccggtt tgttggatgt tgtccgccca cttgaaatcc
     1081 agcgtcgccg gtagcttcgt caggccgagc gccgagcgcg gaacggccaa ctccagctcg
     1141 ttgcccgcgg cgcggcacgg cacgtccagc ggtgatcccc agcgatagcc gccggtgttg
     1201 cgttcgaggg tggtggtttg ggcgtgaacg ttgacccggt tcacgatgaa atcatagccg
     1261 agccagcccg tgcgggcgtc gtggtccgcg tcaatgaaga gaagcatcca attggggtcg
     1321 ctggagggcg tgagcggctc tcgcgtgcgc acatagaaat agacggtggt ggcgtcaagg
     1381 ctgaccttcg cggcgatgat gtcgttgcgg ccggtccggt tgacgtaacg ctggccttgg
     1441 gcccagccgg cgtggtcgcg tttaacgggg tcgccgatgg tgtcgcggaa ttcgggctcg
     1501 accgccgccc agtccccgaa gcgcccgtca atggtgatcg gctgcggctt gaccggcgac
     1561 agcgggcgga cgcctttgta acggcggatg ttggcgatca tctggtaata gtagtcatcg
     1621 ccgtgcccgc ctttcaccgg ctcgatgtcg cggctgaact caccgttgaa ctggtccacg
     1681 aaggtcaccg gtccgggggc gtgaaagggc gcttggtggt cgaagcgcat cgcgatccat
     1741 tcattccagc cggtgatgaa gacgaactcg ggatcaacct ggagcgcgcg gcgccactgt
     1801 tcggcgaagt tgcgcccgga atagtcgcag tctgccggcg ccggctcgcg gccatcgtga
     1861 aagctgcggc cgagtgagcg gggattgctg aggacgctca gcttgccgtc cacggcattt
     1921 tgggcgacgc cgaccgtcat ctgttcccgc gccccggcgg cattggtgaa gacgtgctgg
     1981 ggatacacct ccagccaggc ccattggttc ggcccggtgg gtccgacgaa gtaatccggc
     2041 tgcggcttcc gaaacgtgaa ccggccgcat atctctggcc cgagatcggt ctgggcggga
     2101 tcggccagga tgagcggttt gccctcccag cggaaccaga gatcgggata caaccccggg
     2161 cgatagagct gttcccagag ttcgcgcgca actttcttcg aatcccagaa agggcacaga
     2221 aaggcgattt gcggtgtctg tccgccttcc cgccggatct ggtcgtacac gcggcacaag
     2281 gtcttccagc ttctcgggta ggtctgctgg ttggtgacat cgaacacgat cacgtccacc
     2341 ccggcatccg cgagcatttg cgcgtgcttg cgcagcaccc cctcgtcgtc cgagaggtag
     2401 tagccaaata tggattcacc ccagtgatga aactcgtgca tcgggcccca gagcggactg
     2461 gcagcattag taatggcttg cgggtctcgg gcgaggattt gggtgatgtc gaacgcgccg
     2521 aggtccccgt gtgggtagtg ccaaaggaaa tagaaggctc ccaccgcacg atgcttgcga
     2581 ggcaaacccg cttcagagca ggaagggagg ctgcgcccca gggcgtcggt ggccacccag
     2641 gtgtcgctga acgcatccca gcggggatat tgtatgcctg agtagtcgcc gacgtcagtc
     2701 ggcgctatta tcggggcgga aagaacgcgc cgactaacgt cggcggctac attggtttgg
     2761 gccgtgccgg caccgggcgg ttgctgggcg ggcaccgtga gagtcaacgt gagggctaca
     2821 aggcacagca ggaggacctg actcgggcgg gcgaggatgg aatcgttgct ggcaaagtgc
     2881 attctatggc ttaattcagt ttacggctga tgaatcattc gcggcaatcg gcaatctggc
     2941 aagctgtctt gtgtgggttt tcgaggctgt atgtgtgagg cttcaccctc gctgaacggc
     3001 aagggctggg caaattcgac tggcctggta gaatggcggc atgaataaga gccagattga
     3061 acggccagcg ccttcgagat ttcgccgaca ttggctgtta gcgccgggca cgatcttctt
     3121 gaatcacggc tcgttcggcg cctgtcccaa gccggtgctc gaactccagt cggagctgcg
     3181 gcggcaaatg gaagcggagc cagtccagtt tctctggcgc cgatacgagg agcggctgga
     3241 cccagtgcgc gcggaggcgg ccacgtttct gggcgcgcag gcgcgagacc tggtcttcgt
     3301 gaccaacgcc accaccggcg tcaatgccgt cgtgcgctcg ctcgagctgc gccggggcga
     3361 cgagttgctg accaccaacc aggattacaa cgcctgccac aatgtgctgg tcgaagccgc
     3421 gcaccggact ggggcgaaac tggtgacggt ccgggttccc tttccgttgg cctcgcccga
     3481 cgagctggtc gaggcggtgc tgcgggcggt gaccccgcgc acccggctgg cgatgctcga
     3541 tcacgttacc agcaacacgg cgctggtcgt gccggtggca cggctcatcc gcgagctgga
     3601 ggcccggggc gtggatactt tggttgatgg ggcccatgcg ccgggcatgg tgtcgcttaa
     3661 cttatccaaa ctctcacccg cttactacac cggcaacctg cacaaatggg tctgcgcgcc
     3721 caagggcgcc gcgttcctct gggtgcgccg ggacaaacag gcgccggtgc agccggccat
     3781 catcagccat gggaacaaca cgccgcggcc cgggcatagc gcctttcagg accggtttga
     3841 ttgggctggc acctttgatc cgagcgcctg gctctgcgtc ggcgaggcaa tccggtggat
     3901 ggcgaagctc tatcctggag gctggcccgc gatccaggaa gcgaatcacg agctggcagt
     3961 gcgcgcgcgg cggcagcttt gccaatgctt gaacgtcgag ccgccttgcc cggaagacat
     4021 gctgggggcc atggccacac tgccgttgcc cgaacgcttc cagggccggc ccaggaaagg
     4081 gaaaattgac gtcgagcaac tgcgcctgta cgacggattt ggcatcgagg tgcccttcat
     4141 ccgcgtgaat gagcgccggt atttccggat ctcggcgcag ctttacaact cgctcgcgga
     4201 atacgattac ctggcggagg ctttgttaag cctgcaacca aggagggtgt atccaggcaa
     4261 gggctcgatc aggcgctgaa gcaggcgggc gccagagaag ggcgcgtttg caggaacaat
     4321 aagaaagatt gacaggacta acgggattag caggattgag gcatatatga atagtgggag
     4381 aatacaattc gtggatttgg tcgctcaata tcagcagatc aaaccggagg ttgacgcggc
     4441 gatggcgaag gtttgctcgc gcggcgattt catcatgggc gaggacgtga agctcttcga
     4501 gcaggagttc gcagctttct gtcaagtgcc gcactgtgtg gcggtcgcca acggcacgga
     4561 ggcgttgaag ctggcgctgc tcgcgtgcgg tgtgggtccg ggcgacgaag tcatcacctg
     4621 cacccatacg ttcatcgcca cggtcttcgc gattcaccaa acgggcgcga agccggtgct
     4681 ggtggattgt gacccacggt tttacacgat tgataccgcc caggttgagc gcgccatcac
     4741 cccgcgcacc aaggcgatca tcccggtgca cctgtacggc caaccggctg acatggaccc
     4801 aatcctggag gtggcaaaga agcacaagct ctacgtgatc gaggacgcgt gccaggcgca
     4861 cggcgccgag tataaagggc ggcggtgcgg cagcctgggg gatattgcgg cgttcagttt
     4921 ctaccccgga aagaatctgg gggcctatgg ggacgggggc gcggtgacga cgaagcgggc
     4981 ggacttggcg gaacgcgtgg cgctgctccg caatcatggg cagaaggtga agtacgaaca
     5041 cctggtcaaa ggcttcaaca gccggcttga cacgctgcag gccgtggtgt tgcggataaa
     5101 gctccgccgg ctggagcaat ggaacgaagc gcgccgaaaa gcggcggcca agtatgatcg
     5161 gctgctggcc gggaagggcc tggtgacgcc gaaggtggcg ccgtatgcca agcccgtgta
     5221 tcacctgtac gtggtgcagg tgccggaccg gaagaaacag caggcagcgt tcgacgcggc
     5281 caacgtctcg catgtgatcc attatccgat tcccgtgcac ttgcagccgg cgtttgccga
     5341 gttgggttac aagctgggca gcttcccggt gaccgaggcg ctcgtgccga agatcatctc
     5401 cctgccgatg ttcgctgagc tgaccgacag ccagattgaa caggtcgccg gagcgtgctt
     5461 gaaggcctga aggaatccca atctccagaa gggaagcggt atgggcgcgg gagcgctcgc
     5521 tcttctggga atgaggctgg cagcgtcatc ggtggcggcc agggctagtt cgatagttgg
     5581 taaaatgcca tgtccacgga gactaaagtc tgtgttgtga cgggcgccgg cggttacgtg
     5641 ggcggccggc ttagggaccg cttggagcgc gagggctggc gagttattgc ctgggcccgg
     5701 cagcccgagc cggacagcgg cggggttgct ttccgtctgg gccaggaggt ggattccaac
     5761 ctgttcaagg gtgtgcgcgc gctggtccat tgcgcttatg atttcggtcc ccggcgctgg
     5821 gaggacatcg ccgcgatcaa cataaccggt tcacaaaagc tcctggaggc cgcccgcaag
     5881 gccggcgtgg ggtccgtagt attcatttcc agtctcagcg cgtttgccgg atgccgttcg
     5941 ctctatggca aagcgaagct ggagctcgag agctttgccc acaccatcgg ggcggctgtg
     6001 attcggcccg gcctggtata tagcgaccat ccggggggga tgtttggccg gctggtaggg
     6061 caggttcgag gtgcgcgctt catcccgatt atgtggggcg gaaagcagtt acagtatctt
     6121 gttcacgatg aggacttgag cagcctcgtc ctgggctgtt tggatggccg ggtgcccggg
     6181 cgggctgaac ccatcaccat cgcccacgat cagggatggg aactgaagga gattctgaga
     6241 caaatcgccc aggggctggg caagcgtgtc tcgtttctac ccgtgccgtg gcagctcgtc
     6301 tggctggcct tgaaatccct cgaagtcgca ggcgtgcgga ccaacttccg cagcgacagc
     6361 ctgatcagca tgatttacca gaatccacgc ccttccttcg cttggctgaa gcccttgggt
     6421 tttcagtgcc ggccttttca ctgggctcca acatcctcag gcgagcaagc cactcagtga
     6481 gaaggtgatt ggcgccgcaa aaaaaagagc ggtccggcta tccggaccgc tcccaaccct
     6541 cactcaaacc agaaccagaa ccaagatgaa ccatctaaca acaatgacaa attagcacct
     6601 cgtgcgcata cgtcaatgcg attcttcaca attctttgcg attcttttcc cgcctcccgg
     6661 ttcaattggc cctcaaccgg aagaacctgg ggaaagcgcc aatagcgttg gtgtaggggc
     6721 ttgtcgcgcc aggcacgggc gcgtaggcgc cagtgacggt cgaagccgcc tgcaaaccga
     6781 acgcggagtt ggtccaggtc aggatgagat tgtcagcgct atgcttaacg accaatgaaa
     6841 tgcggttgag aacggcattc gtgccggtaa cggcggcaaa agagaggccc accttcaggt
     6901 cgtcaaccag catcgtcgcc ccgcagccgc tatcctggcg gaagttgaag gaggagatgg
     6961 aaacagcgtt cgtgaggtca gcggcggtcg cgccgcggtc cgattcggag gcggggttga
     7021 gccagagcgt ggctgtggcg gcatca
//
LOCUS       DATCGS010000072         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_649550, whole genome shotgun sequence.
ACCESSION   DATCGS010000072 DATCGS010000000
VERSION     DATCGS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649550"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1611)
                     /locus_tag="VIL39_01440"
     CDS             complement(313..1611)
                     /locus_tag="VIL39_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY5480122.1"
                     /translation="MNTNAQTEAAVETPKKNISVKVLGVGGAGITVMELMRKSGLPGA
                     SFAAVETDAQSLTASCAAEKVHLETQLLRGLGTGGDPDRGRALAEEQLPKLKSLCEGA
                     DAVFILAGLGGGAGTGISPVLARAAKESGALALGFVTTPFECEGSRRQRVAQQGLAEL
                     KSSADGVICLPNQKVFKMIDENTSVLETFKITNDFLADGLRGVWRLLMHKGLIEIHFA
                     DLAALIRNRHAESSFAVAEAMGPTRSREVMDKLLAHPMLDGGQMLGESAAVLVSLIGG
                     PDLTMAEVNRVMEQVNRQCEHAQVVMGAAIDEAFSERLAVTLIAARKNPEHESEEHGA
                     AENPEELDQQLLPNSVTARPGSRFVPPAPDLRSDQVQELFARQSRSSSRQRKNLSKLR
                     QTQLPLEIVSKGRFDKSEPTIHKGEDLDVPTYIRRGIALN"
     gene            complement(1644..>2578)
                     /gene="ftsA"
                     /locus_tag="VIL39_01445"
     CDS             complement(1644..>2578)
                     /gene="ftsA"
                     /locus_tag="VIL39_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY5480123.1"
                     /translation="VSADREISEEDVQDVIKNAKAINLPAQNHVIHAIRQHFLVDGQD
                     GITNPVGMLGARIEVDVHVLHGNFNRLQNAIRTVKGLQLEVDGIVFTGLASSLALLTN
                     EQKELGSLVIDIGGGTTDYVAYSNGIIKHTGVLAVGGDHVSNDLAYGLKVPLSRAEQL
                     KLEYGASFPEGAAKGQSITVSNELGRPLKTVNLDHLRRIMALRLEEIFQLVGQDLEQA
                     GALDYLRAGVFLCGGGAHIPQISKIAEQVLQMPVFLGKTNSISGLKSALDQPEFATAI
                     GLVKFGSFQQRKRAAKSSLTDGIKSTLGQLFRRV"
BASE COUNT          466 a          803 c          797 g          512 t
ORIGIN      
        1 actgatgtat ttgtgcataa attgtgcggg atgattgcca agcgccgccg caattgcaaa
       61 tggaatgaaa agggaggcaa ggctgctcgg atgcgcggcg ggctgaaggg tgaagtagga
      121 ctgcagccct gacatccgaa acccgaagac cgaaggccga aagaaggccg aataccgaaa
      181 tccgaaagtg gctcaatccc atgcgccggt gctccaccga ggtggcttcg gagtcggact
      241 tcggacttcg ggtttctttc ggccttcggc cttcgggttt cggatttagg ctaaatgggt
      301 caggagggcc gttcagttca acgctatgcc gcgccgaata taggtaggca cgtcgaggtc
      361 ctcgcccttg tggatggtcg gctcgctctt gtcaaaccgg cctttggaga cgatctccaa
      421 cggcaactgg gtctgacgca gtttggacag attcttgcgc tggcgcgagc tgctccggct
      481 ctgcctcgcg aagagttcct gtacctggtc cgaccgcaaa tcgggcgccg ggggcacaaa
      541 tcttgagccg ggccgcgccg tgaccgagtt cggcaacagt tgctgatcga gttcttcagg
      601 gttctccgcg gcgccgtgtt cctcggactc atgctccggg ttctttcgcg ccgcgatgag
      661 cgtcacggcc aggcgctcac tgaaggcctc atcaatggcg gcgcccatga ccacctgcgc
      721 gtgctcgcac tggcggttga cctgctccat tacgcgattg acttcggcca tggtcaggtc
      781 tggcccgccg atcaaactca ccagcacggc cgcggattcg ccgagcatct gaccgccgtc
      841 caacatcgga tgcgccagca acttgtccat cacctcgcgc gaacgcgttg gccccatggc
      901 ctcggccacg gcgaaggaac tctccgcgtg gcgattgcgg ataagcgcag ccaggtcggc
      961 gaaatggatt tcaatcagac ccttgtgcat cagcaggcgc cagacgccgc gcagaccgtc
     1021 ggcgaggaag tcattggtga tcttgaacgt ctccagcacg ctggtgttct catcaatcat
     1081 cttgaagact ttctggttgg gcaggcagat cacaccatcg gctgaggact tgagctccgc
     1141 caggccctgt tgcgccacgc gctggcggcg gctgccctca cactcgaagg gcgtcgtcac
     1201 aaacccgagc gccagggcgc ccgactcctt cgccgcgcgc gccagcaccg ggctgatgcc
     1261 cgtgcccgca ccgcctccca gaccggccag gatgaaaacc gcatccgctc cctcgcagag
     1321 cgacttgagc ttgggcaact gttcctcggc cagcgcacgg ccacggtcag ggtcgccgcc
     1381 cgtgcccagc ccgcgcaaga gctgtgtttc caagtgaacc ttttccgcag cgcaagaggc
     1441 cgtgagcgat tgcgcatccg tctccaccgc ggcaaagctc gcaccgggca ggccgctctt
     1501 gcgcatcagt tccattacgg tgatgcccgc gccgccaacg ccaagcacct tgaccgagat
     1561 gttctttttc ggagtttcca ctgccgcttc tgtctgtgcg ttcgtattca tgcgccggtt
     1621 aaagatgctg ttgagggcgg cggtcaaacc cgccggaaga gctggcccag ggtgctcttg
     1681 atgccgtccg tcagggacga tttggctgcg cgcttgcgct gttgaaagga gccgaatttc
     1741 accagcccga tggccgtcgc aaactcaggc tggtcgagag ccgacttcag gccgctgatc
     1801 gaattggttt tgccgaggaa cacgggcatc tgcaggactt gctcggcgat tttggagatt
     1861 tgcgggatgt gcgccccgcc gccgcagagg aacacccccg cgcgcaggta atccagcgct
     1921 ccggcctgtt cgaggtcctg gccaacaagc tggaatattt cttccagccg cagcgccatg
     1981 atgcggcgca gatgatcgag gttaacagtc ttgagcggcc ggccgagctc gttagagacc
     2041 gtaatggact ggcctttggc ggcaccctcg gggaagctgg cgccgtattc gagcttaagc
     2101 tgctcagcgc ggctcagcgg cactttcaag ccataggcca gatcgttgga cacgtgatcg
     2161 ccgcccacag ccagcacgcc ggtgtgcttg atgatcccgt tggaataggc gacatagtcg
     2221 gtcgtcccgc cgccgatgtc gatcaccagg gagccgagtt ctttctgttc gttcgtcagc
     2281 aacgcgagcg aagaagccag gccagtgaag acgatgccat ccacctccag ttgcagcccc
     2341 ttgaccgtgc gaatcgcgtt ttgcagccgg ttgaaattcc catgcaggac gtgcacgtcc
     2401 acctcgatgc gcgcgccgag catgcccacc gggttggtga tgccgtcctg gccgtccacg
     2461 aggaaatgct ggcggatggc gtggatgacg tggttctggg cgggcaggtt gatggccttg
     2521 gcgttcttga tcacgtcctg cacgtcctct tccgagatct cgcggtcggc ggatacaa
//
LOCUS       DATCGS010000073         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_402385, whole genome shotgun sequence.
ACCESSION   DATCGS010000073 DATCGS010000000
VERSION     DATCGS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402385"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="VIL39_01450"
     CDS             <1..2036
                     /locus_tag="VIL39_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480124.1"
                     /translation="GLRISGLNSLVLFTAALFSVLFAAGGAIPPPEQLLPDDTLILLT
                     APDFAKLRAICQKSPKSQLWNDPAMKPLKDKFESRWQEEVIKPIARDLNVSLDTYASL
                     PQGQLTFAVTKNAWQGNDDQPLGFLLLLDAREKTDLLKTNLADLRKKWIGSGKTLKTE
                     TIRNLEFSVFPVTTNDVPKTLSKFLWRPPVFLPVTSGPDMQQAPAPPSSKSDMLLDLL
                     TVLLTASKELVIGQVDSLLVVGNSVKGVEKVVIRLTGGAMPTLGELATYQASHQACFR
                     DAPFYGWVNVKAFTDTLGRRPAETKAPDPADPFEPLKPDQIINSTGLAGCKALAFSLQ
                     NSDEGSLFQLFLSVPEATRQGIFQILVGAAKEASPPPFVPADAVQFFRWRMDGPKTWT
                     TIEKMINDLSPQALNTVNWILDTASARAKLTDPGFDLKKTLLATLGDDIVSYEKAPRG
                     TTPAELESPPYLFLLGSPAPEQLAVTLKRLFVIFPQGDAPAEREFLGRKIFSVPVPPL
                     PFLLAGPSRPRPARTLSCAASGSYVAMSTDTALLEEYLRSSESQAKALREKPDLLEAA
                     QKVGGMSTGLFGYENDAETMRAAFDAVKNDPGASTNGIGPSLFPGLPGITGPEKNLRD
                     WMDFSLLPAFDKVAPYFYFDVYAGSANVDGITLRAFSPVPPALRCYSVAKPAK"
BASE COUNT          422 a          742 c          635 g          381 t
ORIGIN      
        1 tcggccttcg gatttcgggt ttgaactcac ttgtcctatt caccgcagcc ctcttctccg
       61 tcctcttcgc cgccgggggg gccattcctc cgccggaaca attgctgccg gacgacacgc
      121 tcatcctgtt gacggcgcca gacttcgcga agctgcgcgc gatctgccag aagtcgccca
      181 agagccagct ctggaacgat ccggccatga agccgctcaa ggacaagttc gagtcgcgct
      241 ggcaggagga agtcatcaaa cccatcgccc gcgacctgaa cgtgagcctc gatacctacg
      301 ccagcctgcc gcaaggccaa ctgacgtttg ccgtgacgaa gaacgcctgg cagggcaatg
      361 acgaccaacc gctcggcttc ctgctgctgc tcgatgcccg ggaaaagact gatttgctca
      421 agaccaacct cgccgacctc cgcaagaagt ggatcggctc cggcaagacc ctcaagacgg
      481 agaccatccg caacctcgag ttctccgtct ttcccgttac gaccaacgac gtgcccaaga
      541 ccttgagcaa gttcctgtgg cggccaccgg tattcctgcc agtcaccagc ggccccgaca
      601 tgcaacaggc gccggctcct ccgagcagca agtcggacat gctactcgac ctgctcaccg
      661 tgctgctgac agcgagcaag gagttggtga ttggccaggt ggactcgctg ctggtggtcg
      721 gcaattcggt aaagggcgtg gagaaggtcg tcatccggtt gaccggcgga gcaatgccga
      781 cgctgggcga gctggccacc tatcaggcca gccaccaggc ctgctttcgg gacgcgccct
      841 tctatggctg ggtgaatgtg aaggccttca cggacaccct gggccgccgg ccggccgaga
      901 ctaaagcgcc cgacccggct gacccgtttg agccactcaa gccggaccag atcatcaaca
      961 gcaccggatt ggcgggctgc aaagcactcg cctttagcct gcagaactct gacgaaggct
     1021 ccctgttcca actcttcctc agcgtgccgg aagccacccg ccagggtatc ttccaaatcc
     1081 tcgtcggcgc ggcgaaggag gccagtccac cgccgttcgt ccccgcggat gccgtgcagt
     1141 tcttccgctg gcgcatggac ggccccaaga cctggacgac gatcgagaag atgatcaacg
     1201 acctctcccc gcaggccctc aacacggtaa actggattct cgacaccgcc agcgcccggg
     1261 ccaaactcac ggaccccggc ttcgatctca agaagaccct gctcgcgact ctcggcgacg
     1321 acatcgtcag ctatgaaaag gccccgcgcg gcaccacccc ggcggagttg gagtcgccgc
     1381 cctacctgtt cctgctcggc tcgccggctc cggagcaatt ggccgtcaca ctgaagcggc
     1441 tcttcgtaat cttcccgcag ggggacgcgc cggcggaacg ggagttcctg ggtcgcaaga
     1501 tattctccgt tccggtgcct cccctgccct tcctcctcgc gggtccttcc cggcccaggc
     1561 cagcgcgcac gttgagctgc gccgccagtg gcagctatgt cgcgatgtcc accgacaccg
     1621 cgctgctcga ggaatacctg cgcagcagcg aaagccaggc caaggcgctg cgcgaaaagc
     1681 ccgacctgct ggaggcggcc cagaaggtcg gcggcatgag cacgggcctg ttcggttacg
     1741 aaaacgacgc ggagaccatg cgtgccgcct tcgacgcggt gaagaacgac ccaggcgcct
     1801 ccactaacgg catcggaccc agtctcttcc ccggcctccc gggaataacc gggccggaga
     1861 aaaatctcag agactggatg gatttctcgc tgttgcccgc cttcgacaaa gtcgcgccat
     1921 acttctactt cgacgtctat gccggcagcg cgaacgtgga tggcataacg ctcagggcgt
     1981 tttcgccggt cccccccgcg ctccgatgct actcagtcgc caaacccgcc aagtgaccgc
     2041 gaatccagca tgcggttgat gtggtgacat gttatgcgca gacattgcag cattgcatgg
     2101 gagcggcgcg gcgaggcggg aaggccattt ttgcaggcga acattgaatt ccgggtcgaa
     2161 tcttgctttt agcaagcgcg
//
LOCUS       DATCGS010000074         4979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_105809, whole genome shotgun sequence.
ACCESSION   DATCGS010000074 DATCGS010000000
VERSION     DATCGS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105809"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2711)
                     /gene="smc"
                     /locus_tag="VIL39_01455"
     CDS             complement(<1..2711)
                     /gene="smc"
                     /locus_tag="VIL39_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY5480125.1"
                     /translation="MYLKNLTVLGFKSFADKTSLNFQPGVTAIVGPNGCGKSNVSDAI
                     RWVLGEQSAKALRGGEMSDVIFNGTDGRKPLGMAEVSLTIGGVDEEHLIAAGVEMAYN
                     EVTVTRRVFRDGGSEYFINKTPCRLKDIQQLFMGTGVGRTSYSVMAKGNITQILSSKP
                     EDRRMIFEEAAGITKYKTQKREALRKLEYTEQNLLRVADLVREVKRQIGSLQRQAGKA
                     RRYKQLSAELQHLDTQLARHQFDQLQAEIADRQTTADNLRNEIETSSAGALRCEDEIA
                     RLRERVSELEHEIGARQQRGLELKGETDRHESRIHFNEERLREFATQNTKAMADLTQA
                     EERRRAAGEELDTVTRDLAASETALKDHRQTLTARQDALREVEEELRRQQEALRQAQA
                     DAFAAAQDMTRVRNEITALDLQKQGNVVRLEKLSAEKIQLEEERTRLESRLHEFVANV
                     TAQKLNAQTQRGSVEDRQKRLRELQEELARAGRTQDQVLEQQTGKRSRLNVLEQLQAD
                     HEGFSVGPLAVFKQSQHVLGSLADKIRVPDQYVTAIETALGHHLQLVLTEQPESARQI
                     LADLSAKRSGRASIAPLAFVRDGHADADSGDNGQSAPMASPVELNGVPLAAMAVVESE
                     ASIRPLVQRLLGMTRIVRDLNAATAAWRESKGAFHYVTLGGEVLSSHGIYTGGSSNGN
                     GDGKAPASILGRKNQIADLRSALAGLQDEVAEISRRKGALQSEQTGLEAGLQQAQIEL
                     RAQEVAIATREGEFNALQNSQRLLHQKIDTVVYEVQSLAAQEQEGLQKRAGLTAQSGE
                     CESREQGCQSQVVELTARLESLRQQRDTANAGLTDSRVALAAEEQMFASFQQRRQGLD
                     QRLRELTQVIDQRRGELSSFVGRKERAESEIQESQGLIEKLQHDREQV"
     gene            3041..4594
                     /locus_tag="VIL39_01460"
     CDS             3041..4594
                     /locus_tag="VIL39_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY5480126.1"
                     /translation="MPQASSPALAFARRFLRLGLTLAAIGSAFVARAVNYQPSKVIPP
                     APTREFRAAWVATVANIDWPSRKALSTQEQKAELLALLDRAAQLKLNAVIFQVRPACD
                     AMYASRIEPWSEYLTGTMGKPPEPFYDPLTFAIEEAHKRGLELHAWFNPYRARLRAAN
                     SPVAANHISKLRPQLVRQYGEFLWLDPGEKAVQDYSLNVVMDVVKRYDVDGIHFDDYF
                     YPYKVRDGSGKDLDFPDASSWQRFGARGKLSRDDWRRENVNAFIQRLYKSIKATKPWV
                     KFGISPFGIWRPKNPLQISGLDSYAELYADSRKWLVNGWVDYFAPQLYWAIDPPDQSF
                     PVLLRWWAQQNPKGRNLCPGLNSTKVSRSESRQGWQPQEIVNQIRLARAQQGANGHIH
                     WNMKSLMRNTSLDEALEREVYQHSALMPLSPWLGHAQPSKPKVTVATTNPGAQLEIRW
                     TPDGSGKACLWLLQTRTNGQWTQEILPAARTSRAWNRAQPDVVAVSAVNRNGELSAAS
                     VLERPGGAK"
     gene            complement(4621..>4979)
                     /locus_tag="VIL39_01465"
     CDS             complement(4621..>4979)
                     /locus_tag="VIL39_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480127.1"
                     /translation="QKASATAIETETERQMTLAAIALKRFQLRHGQLPPALEALVPEL
                     LPAVPYDYMSAKPLCYRLKPDASYVLYSVGLDGKDDGGDPSSSPGVSPGLWGGRDAVW
                     PSPETEPGKPPRQPDR"
BASE COUNT          888 a         1676 c         1405 g         1010 t
ORIGIN      
        1 acctgttcgc ggtcatgctg gagtttctcg atcaggccct gcgattcctg aatctcggac
       61 tcagcccgtt ctttgcggcc aacgaacgaa gaaagctcgc cacgccgctg atcaataacc
      121 tgcgtcagct cccgcaggcg ctggtccagt ccctgccggc gctgctggaa cgaggcgaac
      181 atctgctctt ccgccgccag cgccacgcgg ctgtcggtca gcccggcgtt cgccgtgtcc
      241 cgctgctgcc gcaggctctc cagccgcgcg gtcaattcca caacctgcga ttggcagccc
      301 tgttcacggc tctcgcattc accggactgg gcggtcagcc ccgcccgctt ttgcagaccc
      361 tcctgctcct gggcggccag gctttgaacc tcataaacta ccgtgtctat cttctggtgc
      421 agcaaacgct gggaattctg gagcgcgttg aactcgcctt cgcgcgtggc gatcgccact
      481 tcctgggcgc gcagttcgat ctgcgcctgc tgcaagccgg cctcgagtcc ggtctgctcg
      541 ctttgcaggg cgcctttgcg gcggctgatt tcggccacct cgtcctggag accggccagg
      601 gctgagcgca aatccgcgat ctggttcttc cggcccagga ttgaagccgg cgctttgccg
      661 tcgccattcc cgttgctgga gccgccggtg tatatgccgt gactgctcag cacctcgccc
      721 ccgagcgtga cgtagtgaaa agcgcctttg ctctcccgcc acgcggcggt cgccgcattc
      781 agatcccgca caatgcgcgt catcccgagc agacgctgca ccagcgggcg gatggaagct
      841 tcgctctcga cgaccgccat agcggccagc ggcacgccat tcagctccac tggcgaggcc
      901 atcggcgcgg actgcccatt atcgccggaa tcggcgtcgg cgtggccgtc tctgacgaat
      961 gccagcggcg cgatgctagc ccggccactt ctcttcgcgc tgaggtctgc cagaatctgg
     1021 cgtgctgact ccggctgctc ggtcaacacc agttgcaagt gatggcccag ggctgtctcg
     1081 atggccgtga catattgatc gggcacgcga atcttatccg ccagcgagcc gagcacatgt
     1141 tgcgactgtt tgaaaacggc gagcgggccg acgctgaaac cctcatggtc ggcctgcaat
     1201 tgttcgagca cattcagccg ggagcgtttc ccggtttgtt gctccaggac ctgatcctgc
     1261 gttcggcccg cgcgcgccaa ttcctcctgg agctcgcgca accgcttctg ccgatcctcg
     1321 acgctgccac gctgcgtttg cgcgttgagt ttctgcgccg tgacgttggc cacgaactca
     1381 tgcagccggc tttccaggcg ggtccgctcc tcttcgagct ggatcttctc ggcggaaagc
     1441 ttctccaggc gcaccacatt gccctgcttc tgcaaatcaa gcgcggtgat ctcgttgcgg
     1501 acacgcgtca tatcctgcgc tgccgcgaac gcatcggcct gggcctggcg cagagcctcc
     1561 tgctgccgcc gcagctcctc ttccacttcc cgcaatgcgt cctgcctggc ggtcagcgtt
     1621 tgcctatggt ccttaagcgc cgtttcggaa gccgccagat cccgggtgac cgtgtcaagt
     1681 tcctccccgg ccgcccggcg ccgctcctcc gcctgcgtca ggtcggccat ggccttggta
     1741 ttctgtgtgg cgaattcgcg caggcgctcc tcgttaaaat ggatgcggct ttcgtgccgg
     1801 tcggtctcgc ccttcagctc cagcccgcgc tgctgccttg cgccgatttc gtgctccaac
     1861 tccgacacgc gctcgcgcaa tcgggcaatc tcatcttcgc accgcaacgc cccggctgaa
     1921 ctggtctcga tttcgttgcg gaggttgtct gccgtggtct gccggtcagc gatctcagct
     1981 tgtagttggt cgaattgatg gcgggcaagc tgggtatcca agtgttgcag ctccgcggaa
     2041 agctgtttgt agcggcgcgc cttgccggct tggcgttgca gcgagccgat ctgccgtttc
     2101 acttcccgga ccaggtccgc cacgcgcagc aggttctgct cggtgtactc cagcttgcgc
     2161 agcgcctctc gcttttgcgt tttgtacttg gtgatcccgg cagcctcttc aaagatcatc
     2221 cggcggtctt ccggcttgct ggacaggatc tgcgtgatgt tccccttggc catgacactg
     2281 taactggtcc gccccacccc tgtgcccatg aacagttgct ggatgtcttt caaccggcac
     2341 ggtgtcttgt tgatgaaata ctcgctcccg ccgtcacgga aaacccggcg cgtcacggtg
     2401 acctcgttat acgccatttc gacgcccgcc gcgatcaggt gttcctcgtc caccccccca
     2461 atcgtcaacg aaacctccgc catccccagc ggcttgcgac cgtccgtgcc gttaaagatt
     2521 acatccgaca tctccccacc ccgcagcgcc ttggcggatt gctcacccag tacccaacgg
     2581 attgcgtccg agacattcga cttgccgcac ccattggggc ccacaatggc ggtcacccca
     2641 ggctgaaagt tcagcgacgt cttatctgca aaggacttga atcctaagac ggttaaattc
     2701 ttgagataca tgatcttgct aaagagaacc aaatctgccc tgcttagtaa agtggaaaaa
     2761 ccacaatata cagggcacac ctgccgtcgg aacacaacta attggtgagg aaccgagatt
     2821 tgcgttgtgc cgccaaaacc gctaatcaaa ctctataaac tacatcaacc acccataatc
     2881 tagcgcaaac tacccccgtt tggcaacgac ttttcgcaac aaactttacc ctcgatattg
     2941 gccccgttgg gtcatactct gctccccagc aagtgacttt atgagcataa acagcctccg
     3001 ctcgaactcc ggtcttgtgc aaaggactgg acaacgccaa atgccgcagg cctcctcccc
     3061 tgccctcgca ttcgctcggc gcttcctccg ccttggcctg acgctggcgg cgatcggttc
     3121 agccttcgtg gcccgggctg tgaattacca accatcgaag gtcatccctc ccgcaccgac
     3181 acgcgaattc cgcgcggcct gggtcgccac ggtcgcgaac attgactggc cttcccggaa
     3241 agcgctgagc acgcaggagc agaaagccga gctgctcgcc ctgctcgacc gggctgccca
     3301 gctcaagctc aacgccgtca tcttccaggt caggccggcc tgcgacgcca tgtatgcctc
     3361 tcggattgag ccgtggtccg agtatctcac gggcacgatg ggcaaaccgc cggagccgtt
     3421 ttatgatccg ctgaccttcg caatcgagga agcgcacaaa cgcgggctcg agttgcacgc
     3481 ctggtttaat ccttaccgag cgcggttgcg cgccgccaac tcgcccgtcg cggccaacca
     3541 catcagcaag ctccgcccac agcttgtccg gcagtatgga gaattcctct ggctggaccc
     3601 gggcgaaaag gccgtgcagg actactcgct gaatgtggtg atggacgtcg tcaagcgcta
     3661 cgacgtggat ggcatccact ttgacgacta cttttacccc tacaaggtac gcgatggctc
     3721 cggaaaggat ttggatttcc ccgacgcctc aagctggcaa cggtttggag cccgcggaaa
     3781 gttgagccgc gacgattggc ggcgcgagaa tgtcaatgcg ttcattcagc gcctctacaa
     3841 atccatcaag gccacgaagc cctgggtgaa gttcggcatc agccccttcg gcatctggcg
     3901 gcctaagaat ccactccaga ttagcgggct ggactcctac gcagagctct atgccgattc
     3961 acgcaaatgg ctggtcaacg gctgggtgga ttactttgct ccgcagcttt attgggcgat
     4021 tgacccgccg gaccaaagct tccccgtgct gctgcgctgg tgggcccagc agaaccccaa
     4081 gggccggaac ctctgcccgg gcctgaactc caccaaggtc agccgatcgg agtcgcggca
     4141 gggctggcaa ccccaggaaa tcgtcaacca gattcgcctg gcccgcgcac agcagggagc
     4201 taacggccat attcactgga acatgaaaag cctcatgcgc aacacctcgt tggacgaagc
     4261 cctggaacgg gaagtgtacc agcattccgc gctgatgccg ctttcaccct ggcttggcca
     4321 tgcccagcct tcaaaaccaa aggtaaccgt cgccaccacc aatcccggcg cgcaactgga
     4381 aatccgctgg acgccggacg gctcaggcaa ggcctgcctc tggctgttgc agacacggac
     4441 caacggccag tggacgcagg aaattctccc agccgccagg accagccgcg cttggaatcg
     4501 cgcgcaaccc gacgtcgtgg cggtctcggc cgttaaccgt aacggcgagt tgagcgctgc
     4561 cagcgtgctc gagcggcccg gcggcgcaaa atgatccgcg attcgattga atgccggccc
     4621 ctaacgatcc ggctgccggg gcggcttgcc tggctcggtc tcgggagatg gccaaacggc
     4681 atcccgcccg ccccataaac caggcgagac gcccggtgac gagctcgggt cgccgccgtc
     4741 atccttcccg tccagaccaa cggaatagag cacataactc gcgtcgggct tgaggcgata
     4801 acagagcggt ttggcgctca tgtaatcgta gggcaccgcc ggcaaaagct ccggcaccag
     4861 cgcctccagg gcgggcggca gttggccatg ccgcagttga aagcgcttca gtgcgatggc
     4921 agccagggtc atttgccttt cggtttccgt ctcgatagct gtggcgctcg ccttctggt
//
LOCUS       DATCGS010000075         6882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_303362, whole genome shotgun sequence.
ACCESSION   DATCGS010000075 DATCGS010000000
VERSION     DATCGS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303362"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..591
                     /locus_tag="VIL39_01470"
     CDS             202..591
                     /locus_tag="VIL39_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEY5480128.1"
                     /translation="MLNTGILNPQILSLLARVRHTNALVIADRGFPFWPSIETVDISL
                     MDNLPTVLQVLTALRPNFRIAQAYMAREFLRENTPATRALYTKALQGISTTHEPHVQF
                     KKRVPGAIGLIRTGDTVQYANMVLISG"
     gene            847..1188
                     /gene="hypA"
                     /locus_tag="VIL39_01475"
     CDS             847..1188
                     /gene="hypA"
                     /locus_tag="VIL39_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEY5480129.1"
                     /translation="MHELSIMQSALSLALDQARQAGAVRVHVIRLRIGALSGVVPEAL
                     EFAFEALTPGTLAEGAKLAIEPVPARFWCATCTQEFQSDNMFAECPGCHSPSGDLRAG
                     REMELASLEID"
     gene            1188..2075
                     /gene="hypB"
                     /locus_tag="VIL39_01480"
     CDS             1188..2075
                     /gene="hypB"
                     /locus_tag="VIL39_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEY5480130.1"
                     /translation="MCKDCGCGLPGETPIGVSAHHHEHEHEHGHEQGPGEHHDHDHPH
                     PHAHSHTHEHSHTEADHDHTHEAGDEARRTVEVRRAILDKNDRLAERNRGFFRARGLL
                     VLNFLSSPGSGKTTFIRESVRKLGPELKTGVIVGDLATDNDAQRLRESGAPVVQITTG
                     TVCHLEAEMVARAVKQLDLAGLNLLIIENVGNLVCPASYDLGEDMRVVLLSVTEGEDK
                     PLKYPPMFQSADVVIISKIDLAQVCGYDRDTALANIRRVAPKARVIETSAKTGQGLDA
                     WREFLLRQQQARKALDPLG"
     gene            2344..3324
                     /locus_tag="VIL39_01485"
     CDS             2344..3324
                     /locus_tag="VIL39_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5480131.1"
                     /translation="MSTPLNEQVQAAAGWIGRLRTEVGRVIVGQEQLVDRLLVGLLAN
                     GHVLLEGVPGLAKTLCVRTMASAIHATFRRIQFTPDLLPADITGTLIYSPQDGTYHAT
                     KGPVFANFVLADEINRAPAKVQSALLETMQERQVTLGGETMPLPVPFLVLATENPIDQ
                     EGTYPLPEAQVDRFMFKVLLDYPSFEEERKILDRMAFTAPETQVQPVIPLEEILQTRK
                     LVDQIHVDEKVRDYVVHLVFATRQPEKYKLDLKHFIQFGASPRATIYLTLAAKAWALL
                     QGRTYVTPEDVKSIGPDVLRHRLILTYEAEAQAVTTDAIIKKIFNAVPVP"
     gene            3497..4414
                     /locus_tag="VIL39_01490"
     CDS             3497..4414
                     /locus_tag="VIL39_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5480132.1"
                     /translation="MTTTELLESVRRIEVRTNRLVNDTMVGAYLSHFKGRGMDFEELR
                     EYIPGDEVRDIDWNVTYRMGRPFVKRYREERELAMVLAVDVSASSAFGSLRRTKRQFA
                     AEIAGTLAISAARSSDKVALLLFTDQVELFLPPRKGRRHILRLIREMLSFEPKHRGTN
                     IPAALAFLNRVLHRRSIVFLLTDFLHSFGPALAQPVAGRGTLDEIGLTNARHDLVCMH
                     LHDPRESTLPTAGLLTIEDAETGELLELDSSRAAVRERFANTNAERLADLDRAFRRAG
                     VDTLRFSTAEPFAQTLQTFFETRRGRRRG"
     gene            4411..5211
                     /locus_tag="VIL39_01495"
     CDS             4411..5211
                     /locus_tag="VIL39_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HEY5480133.1"
                     /translation="MKGRNPRAEGRKKAEIRRPKSEEQSASGTCPPPAAAAGTPTGYT
                     KFLFDFRISAFFRPSDFGLRISTPALWLLIPALPLLAATPTSPDAIPPLRPPHAEMPP
                     TFWDQYSLWVILFGVLLLALVCAVAWVLTRPKPPMVVPPEVQARQALEPLRQQPEDGV
                     LLSRVSQILRHYVAAAFGLPPGELTTAEFCRAIDGHAQIGPELSAALSEFLRQCDQRK
                     FSPSAPVPPLGAVAQALKLIDQAQARCLAQARSVGNAHVSPSKTAEVK"
     gene            5324..6364
                     /locus_tag="VIL39_01500"
     CDS             5324..6364
                     /locus_tag="VIL39_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5480134.1"
                     /translation="MTGNLQFQYPWLLALLALLPIYAFLRGRIGKLAALRFPSADIAR
                     AAGAAARAASGRFLLFLRLLTVALCIVALAGPRFANDHTETQASGVDIMLVLDLSWSM
                     MALDMGGPQERVSRYDIASQVLEDFIRKRPSDRLGLLVFSAVPYLASPLTLNHAWLIE
                     NLRRLHVGLIRDLGTAIGDATAAGAKRLKSLKDSKSRIIILLTDGDNNKGDIDPVPAA
                     QLAAALGVKVYTIGIGIEQPCALPLFDPSTGKLELDPNGNLKPGMILQPANYDVLGKM
                     ATLSRAKFYRATNRRELQRIYNEIDQLEKTEIKLRRFTTYRPLYQWPLLAAFGLFALE
                     MGLANTRLRRVP"
     gene            6567..>6882
                     /locus_tag="VIL39_01505"
     CDS             6567..>6882
                     /locus_tag="VIL39_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480135.1"
                     /translation="MNFANPHFAEPRFLWLAVLAPLLLATLQRYSAWARQKQLAQIAA
                     PHFVEELTRSVSPARRAIKQALLILAAAGVGLALARPQWGEQAEVSYLFGQDTLFILD
                     CSR"
BASE COUNT         1253 a         2344 c         2032 g         1253 t
ORIGIN      
        1 aatgaagcac ctcctttgcg ccaaaggtcc ttcatgaatt ggcgctggga ggtcgcggtc
       61 ctcgggtttt gctacttgcc acaggggtcc gtatttcaga aagtcatcgt gaatcgctgg
      121 caggcatcgc gctgcgagcg cgggcgcagg caactcatgc aactcatttg gaccatgtaa
      181 cgcgagccga ctcaggctgc tatgctcaac accggaatcc tcaacccaca aatcctctcg
      241 ctgctggcgc gggtgcgcca taccaatgcc ctggtgatcg ccgaccgtgg ctttcccttc
      301 tggccaagca tcgagacggt ggacatctcc ctcatggaca acctcccgac ggtcttgcag
      361 gtgctgaccg ccctccggcc caatttccgt atcgcccagg cctatatggc gcgcgagttc
      421 ctgcgcgaga acacgccggc cacgcgagcg ctgtacacaa aggccttgca aggcatttcc
      481 acgacccacg agccgcatgt gcagttcaag aaacgcgtgc cgggcgccat tggcctaatc
      541 cgcaccggcg acacggtcca atacgcgaac atggtcctga tttccggctg aaggattcgc
      601 ctgcgtggcg ttctacggtt tcaactggcc ggagatcagc aaaacggagt cgctgaacgg
      661 agtgggaact gcgggcggcc attgagctgg ttgcccggcc cggaaatcgg ggcgtcggcc
      721 ctgccaaaga aacgagcggc cccacctggg ccgctccgct aggcgcatct acggttgcgc
      781 tgttcctctg ccgaacaatt cgtttgtcag gcgcaggggg gcgacctacc ttggttagtc
      841 ggacccatgc acgagttgtc aattatgcag agcgcgctga gcttggcgct ggaccaggcg
      901 cgacaggctg gggccgttcg tgtgcatgtg attcggctgc gcatcggggc gctcagcggg
      961 gtggtgccgg aggcgctgga gttcgccttc gaggccctga caccggggac actggctgaa
     1021 ggcgccaaac tggccatcga gcctgtccct gcgcggttct ggtgtgcgac gtgcacccag
     1081 gaattccagt ccgataatat gtttgccgaa tgccccggtt gtcacagtcc gagcggagac
     1141 ctccgcgccg gccgggaaat ggaactagcc tctttggaga ttgactgatg tgtaaagatt
     1201 gcggatgcgg attgcccgga gaaacgccga taggcgtaag cgctcatcac cacgagcatg
     1261 agcatgaaca cgggcacgaa cagggtccgg gcgagcacca cgaccatgac catccccacc
     1321 cgcacgcgca ctctcacacc cacgagcact cgcacactga ggccgatcac gatcacaccc
     1381 acgaggctgg cgacgaagcg cgccggaccg tcgaggtgcg ccgtgccatt ctcgataaga
     1441 acgaccgcct cgctgagcgc aaccgcggct tcttccgagc ccgcggcctg ctggtgctga
     1501 actttctgtc ctctcccggt tcgggcaaga cgacctttat tcgcgagagc gtgcggaagc
     1561 tggggccgga actgaagacg ggcgtgatcg tcggcgacct ggcgacggac aatgacgcgc
     1621 aacggttgcg cgagtcaggc gcgccggtcg tgcagatcac caccggcacc gtttgccatc
     1681 tcgaagccga aatggtggcc cgtgccgtca aacaactgga cctggccggc ctcaacctgc
     1741 tgattatcga gaatgtcggc aacctggtct gcccggcctc ctacgatttg ggtgaagaca
     1801 tgcgagtggt gcttttgtcc gtcaccgaag gggaggacaa gccgctaaag tatccgccca
     1861 tgttccaatc ggcggacgtg gtaatcatca gcaagattga tctcgcccag gtgtgcggct
     1921 acgaccgcga cacggcgctg gccaacatcc ggcgcgtggc gcccaaggcg cgagtaattg
     1981 aaacgtccgc caagaccggg caaggcctcg acgcctggcg cgagtttctg ctccgacagc
     2041 aacaagcgcg caaggcgctc gaccccctgg gctgaaccgc gcgggcagcg gagatgggag
     2101 aaaaagaggg aaaaaacccc gaatcccttc gtaactccat cgtaacccca tcgtaacccc
     2161 ttcgaagccc catcgtaaca tcacgctcac aacaccctcg ctgcgccgta gcacccccct
     2221 tgcagcaggc tgggcggtgg cggagcgtgc cggagccgga cggtgggggc ctggggagtt
     2281 ggcggagttg aaacgcttag ataacttgag tcactgcgca ttcttgccta gattcatcga
     2341 atcatgagta caccgttgaa cgagcaagtg caggccgccg ccggctggat cggcaggctg
     2401 cgcacagagg ttggccgggt catcgtcggc caggagcaac tggtggaccg gttgctggtc
     2461 gggttgctgg ccaacggcca cgtcctgctc gaaggcgtgc ccggcctggc caagacccta
     2521 tgcgtccgca ccatggcctc cgccatccat gccaccttcc gtcgcatcca gttcacgccc
     2581 gacctgctgc ccgctgacat cacgggcacg ctgatttaca gtccccagga cggcacgtat
     2641 cacgccacca aaggccccgt gttcgccaat ttcgtgctgg ccgacgaaat caaccgcgcg
     2701 ccggccaaag tgcaatcggc cttgcttgaa accatgcagg aacggcaagt gacgctcggc
     2761 ggcgagacga tgccgctgcc ggtaccgttc ctggtactgg cgacagagaa cccgattgac
     2821 caggaaggca cctatccctt gcccgaggcc caagtggacc gcttcatgtt caaggtgctg
     2881 ctcgactatc cgtccttcga ggaggagcgc aagattctcg accgcatggc gttcaccgcg
     2941 cccgagacgc aggttcagcc ggtcatccca ctggaggaaa tcctgcagac gcgcaagctg
     3001 gtggaccaga ttcacgtgga cgagaaggtc cgtgactacg tggtgcacct cgtcttcgcc
     3061 acccggcagc cggagaaata caagctcgac ctgaagcatt tcatccagtt cggcgcctcg
     3121 ccgcgggcaa cgatctactt gacgctggcg gccaaagcct gggcgctgtt gcagggccgg
     3181 acctacgtga ccccggaaga cgtcaagagc atcggccccg acgtgttgcg gcaccggctc
     3241 atcctcacgt atgaagccga ggcgcaggcg gtgacgaccg acgccatcat caagaagata
     3301 ttcaacgctg tgcccgtgcc gtgatgacaa atccgaaacc cgaaacccga aggccgaaag
     3361 aaggccgaaa tccgaaggcc gaactcggca ctccgctgct gtctcctgcc tcctgctcat
     3421 tcgggtttcg gatttcggta ttcggatttc tttcggcctt cgggtttcgg gtttcggatt
     3481 tcggccccgc cttgtcatga ccaccaccga gttgctcgaa agcgttcgcc gcatcgaggt
     3541 gcgcacgaac cgcctggtca acgacaccat ggtgggcgcc tacctgagcc acttcaaggg
     3601 ccgcggcatg gacttcgagg agttgcgcga gtatatcccc ggcgatgagg tccgcgacat
     3661 tgactggaac gtcacctatc gcatggggcg tccgttcgta aagcgctacc gcgaggagcg
     3721 cgaattggcg atggtgctgg ccgtggacgt ttcggcctcc tcggcgttcg ggtccctgcg
     3781 ccgcaccaag cgccagtttg cggcggagat tgccggcacg ctggccattt ccgccgcgcg
     3841 gagcagcgat aaggttgcgc tgctgctgtt caccgaccag gtcgagttat tcctgccgcc
     3901 gcgcaaagga cggcgccaca ttctgcggct catccgcgag atgctctcct tcgagccgaa
     3961 gcaccgcggc accaacatcc cggcggcgct ggccttcctg aatcgcgtgt tgcaccggcg
     4021 ctcgatcgtg ttcctgctca ccgactttct acatagcttt ggccccgccc tcgcccaacc
     4081 cgtcgccggt cgcggcaccc tcgatgaaat cgggcttacc aacgcccgcc acgacctcgt
     4141 atgcatgcac ctgcatgacc cgcgggagag caccttgccc accgccgggc tgctgacgat
     4201 cgaggacgcc gagacgggtg agttgctgga actcgattcc agccgcgccg ccgtgcgcga
     4261 gaggtttgcc aataccaacg ccgagcgcct cgccgacctc gaccgcgcct tccgtcgcgc
     4321 cggggtggat accctgcgct tcagcaccgc cgaacccttt gcccaaacac tccaaacttt
     4381 cttcgagacc cggcgcggga ggaggagggg atgaaaggcc gaaacccgag ggccgagggc
     4441 cgaaagaagg ccgaaatccg aaggccgaag tccgaagagc aaagcgcctc cggaacgtgc
     4501 ccacccccag cggcagcagc cgggacgccc acgggctaca cgaagttctt gttcgacttt
     4561 cggatttcgg ccttctttcg gccttcggat ttcgggcttc ggatttcaac ccccgccctc
     4621 tggcttctca tccccgctct cccgcttctc gccgccacgc cgacatcccc ggacgcgatc
     4681 cctcccctgc gtccgccaca tgccgagatg ccgcccacct tctgggacca atacagcctg
     4741 tgggttatcc tcttcggggt gctcctgctg gcgctggttt gcgctgtggc ctgggtcctg
     4801 acgcggccca agccgccaat ggtcgtgcca ccggaggttc aggcccgcca ggcgctggag
     4861 ccattgcgcc agcaaccgga agatggtgtg ctgctgagcc gcgtctccca aatcctgcgc
     4921 cattatgtgg ccgcagcctt cggcctgccg cccggcgaac tcacgaccgc ggagttttgc
     4981 cgcgccatag acggccatgc gcagatcggc cccgaactct cagccgcgct cagcgagttc
     5041 ctccgccaat gcgaccagcg caaattctcc ccgtccgctc cggtcccgcc gttaggtgcg
     5101 gtcgcccagg cactcaagct gattgaccag gcccaggccc gctgcctcgc ccaggctcgg
     5161 tcagttggaa atgcacacgt tagtccatcc aagaccgcag aggtgaaatg aacttttcca
     5221 aagcacctca gcgctcggat ttggcacttc ggcattcgga cttcggagtt cggccttctt
     5281 tcggccttcg gacttcggcc ttcggacttc ccccctccct gcgatgacgg gcaacctcca
     5341 gttccaatac ccctggctgc tggcgttgct ggcgttgctg ccgatctacg ccttcctgcg
     5401 cgggcgtatc ggcaagctcg ccgcgctgcg ctttcccagc gccgacatcg cgcgcgctgc
     5461 gggcgccgcc gcgcgggccg cctccggccg cttcctgctt tttctgcggc tcctcaccgt
     5521 ggccttatgt atcgtggcgc tggccggacc gcgcttcgcc aacgaccata ccgagacgca
     5581 ggctagcggc gtggacatca tgcttgtgct cgatctctcc tggtcaatga tggcgctgga
     5641 catgggcggt ccgcaggagc gggtctcgcg ctatgacatc gcctcccagg tgctcgagga
     5701 cttcattcgc aaacgcccca gcgaccgcct tggcctgctc gtcttctccg ccgtgcccta
     5761 cctggccagc ccgctcaccc tcaatcatgc ctggctcatc gagaacctcc gccgcctcca
     5821 cgtgggcctc atccgcgacc tgggcacggc catcggcgat gccactgccg ccggcgccaa
     5881 gcggctaaag agcctcaagg acagcaagag ccgcatcatc atcctgctga ccgatggcga
     5941 caacaataag ggcgatattg accccgtgcc cgccgcgcag ctcgccgccg cgctgggggt
     6001 gaaggtctat accatcggca tcggcatcga gcagccgtgc gctttgcccc tgttcgaccc
     6061 gtccaccggc aagctggagc ttgaccccaa cggcaacctc aaacctggca tgatcttgca
     6121 gcccgccaac tacgacgtgc tcggcaaaat ggccacgctc tcccgcgcca agttctaccg
     6181 cgctaccaac cgccgcgaac tccagcgcat ctacaacgag attgaccaac tggagaagac
     6241 tgagatcaaa ctccggcgct tcaccaccta ccggccgttg tatcaatggc ccctgctcgc
     6301 cgccttcggc ctgttcgccc tggagatggg tctcgccaat actcgcttgc ggagggtgcc
     6361 atgatgcgaa atccgaaacc cgaaacccga aacccgaagg aaggccgaaa tccgaaggcc
     6421 gaaatcccaa ggtcaaagtc cgaacccggt agtccgcttc tggcccctag cgccggctcc
     6481 tgtttcggat ctcggttttc ggtattcgga tttccttcgg ccctcgggtt tcgggcttcg
     6541 gatttcggcc cttacggccg ccgcgcatga acttcgccaa tccccatttc gccgaaccgc
     6601 gctttctctg gctggccgtg ctggcgccgc tgctgctggc caccctccaa cgctattccg
     6661 cctgggcgcg acagaaacag ctcgcccaga ttgccgcacc gcacttcgtc gaagagctga
     6721 cccgctcagt cagccccgcc cgccgcgcca tcaaacaggc gttgctcatc ctcgccgcgg
     6781 ctggggtggg actcgccctg gcgcggccgc agtggggcga gcaggcggag gtcagttacc
     6841 tgttcggcca ggacaccctc ttcattctgg attgctcccg ca
//
LOCUS       DATCGS010000076         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_452041, whole genome shotgun sequence.
ACCESSION   DATCGS010000076 DATCGS010000000
VERSION     DATCGS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452041"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VIL39_01510"
     CDS             complement(<1..339)
                     /locus_tag="VIL39_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480136.1"
                     /translation="MKSWFAKFRISAAMDSGQELEAGSQRRTSVSDELRGFEQEMAAL
                     DGALKQTAPRSQAPPSLHGSIMRAVRAAEQPVAEARRGLTFLRWLSVPVVAVLALLVV
                     WPVARGPVRVL"
     gene            complement(336..1052)
                     /locus_tag="VIL39_01515"
     CDS             complement(336..1052)
                     /locus_tag="VIL39_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480137.1"
                     /translation="MATDFQRCSDEELARQSQDGSLAAFEELVYRYEGRIYGFVVNAC
                     RNVADAREVTQDTFVRAFQAIGQFDCGRGFGPWLFTIARRKCIDHHRAAPPAADEPMP
                     ELADHNDPSELLARREERQGLWDMARRRLPEPQFQVLWLRYAEEMSMAGIAQVLRKTQ
                     THVKVLLFRARQVLGRELRAGQAHSLPIGRVASRPAPDGEARRSLFDGVGSGGRVSGV
                     VELSEAAVSSEPRNARKGPV"
     gene            complement(1195..2157)
                     /locus_tag="VIL39_01520"
     CDS             complement(1195..2157)
                     /locus_tag="VIL39_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480138.1"
                     /translation="MNANFLRTAFCACGLAAVGLPSFAGPLQRADVPAEPSWVLHVDC
                     DCLRPTAIGQYLLAEMEKPEAAAKLAEVQTMFGFDLRKQLHGLTLYSTGKVPQEGVLL
                     VYGDFDADRLIKMVSGAQDYQTTPYKQHVIHNWIDDKKKAVEGIKPRVYGAIQGGRIV
                     VLAQQEARVAQALDVLDRTTPNLAASGVFPQLGASGSTSIIQAAARKLDIPDSTPNAA
                     ILRLAKAARLQVGEGQGQLRATLNLETNDPQVAQQMATVGQGLVALLKLQKFNPSSSK
                     LAEALSLKQEGAGVVATLTMPTTDVIELVKADAARKAQKKAEKE"
BASE COUNT          448 a          843 c          664 g          464 t
ORIGIN      
        1 tagcacccgc accggaccgc gcgccacagg ccagaccacc agcagcgcca gcaccgcgac
       61 gacaggcaca gaaagccagc gcaggaaggt cagcccacgt cgcgcttccg ccaccggttg
      121 ctcggctgcc cgcaccgccc gcataatcga gccatgcaac gatggcgggg cctgagacct
      181 cggcgctgtc tgcttcaagg cgccgtccag cgccgccatc tcctgctcga agccgcgcag
      241 ctcatctgaa acgcttgtcc tccgctgcga gcctgcttcc agctcctggc cgctatccat
      301 cgccgcggag atcctaaact ttgcaaacca tgatttcata cgggtccttt cctggcatta
      361 cgaggttccg aactgactgc cgcctcggac aactcaacca cgccgctcac ccttccgccg
      421 ctccccaccc catcgaatag cgagcgcctt gcctccccat cgggtgcggg ccgggaagcg
      481 acacgcccga tcggcaagct gtgcgcctgc cccgccctaa gctcgcggcc caacacctgg
      541 cgcgcacgga acagcagcac cttcacgtgc gtttgggtct tgcgcagcac ctgcgcaatg
      601 cccgccatgc tcatctcctc agcgtatctc agccaaagca cctggaactg cggttccggc
      661 aaacgccggc gcgccatgtc ccacaagcct tgccgctcct cccgccgcgc cagcaactcc
      721 gacggatcat tatggtccgc cagttcaggc atcggttcat ccgccgcggg cggcgcagct
      781 cggtgatggt caatgcactt ccggcgggcg atggtgaaca gccacgggcc gaaaccgcgt
      841 ccgcaatcaa actggccaat cgcctgaaag gcccgcacga acgtgtcttg cgtcacttcc
      901 cgggcatcag cgacgttccg gcacgcattg acaacgaacc catagattcg cccctcgtag
      961 cgatagacca actcctcaaa tgccgccagc gagccgtcct ggctttgccg agccagctcc
     1021 tcatccgagc atctctggaa atcagtagcc acctggcatt cctggaactc tcaatttcac
     1081 acttcgcacc actccccaac gaaccatagc cgccgacgtc agtccgcgca ttcttctggc
     1141 ccttaagata gcgcggactg aggccggcgg ctactcgggc tttgaatgcg agagctattc
     1201 tttctccgcc ttcttctgcg ctttgcgggc ggcgtcagct ttcaccagct cgatgacatc
     1261 ggtggtcggc atggtcagcg tggccaccac acctgcgccc tcctgcttca gcgaaagcgc
     1321 ctcggcaagc tttgacgagc tgggattgaa cttctgcagc ttcaacagcg cgaccaggcc
     1381 ctgcccgacc gtggccatct gttgggccac ctgcggatca ttggtctcaa ggttcagagt
     1441 cgctcttaac tgcccctgcc cttcgccgac ctgcagacga gccgccttcg ccagccggag
     1501 gatggccgcg ttaggagtcg agtcaggaat atccagtttg cgggcggctg cctggatgat
     1561 gctggtgctg ccgctggccc ccaattgcgg gaacacgccg ctggcggcga gatttggcgt
     1621 cgtccggtcc agcacgtcca gcgcctgggc cacgcgcgct tcctgttggg caagcacgac
     1681 gatgcgccca ccctggattg ccccgtacac ccgcggcttg atgccctcca cggccttctt
     1741 cttgtcatca atccagttgt gaatgacatg ctgcttataa ggagtggttt ggtaatcctg
     1801 cgcccccgag accatcttga tgagccggtc tgcgtcgaag tcgccataca ccaacaacac
     1861 cccctcctgc gggaccttgc cggtgctgta gagcgtcaga ccgtggagct gcttgcgcag
     1921 gtcaaagccg aacatggtct ggacctcggc caacttggcc gctgcttccg gcttttccat
     1981 ctccgccagg aggtattggc caatcgccgt tggccgcaag cagtcgcaat caacgtgcag
     2041 cacccaagac ggttccgccg gcacgtcggc ccgttgcagc ggtccggcaa aggaaggcaa
     2101 tccgacggcg gcgaggccgc aggcgcaaaa agcagttctg aggaaattcg cattcatttg
     2161 tataggctgt tattgtcgtt cagttcttcg ttgccaacga ggcaatccaa tgcctcgctt
     2221 caatgaagga tacgctgaaa acccgccact ggttacagaa ttccattgac ttcccccacc
     2281 ccgcggtcct cccaccgaaa tcgccaacgg caagaaatcg aggaacaacc ggctttccca
     2341 cgcctttggg gtggccttgg agtagccttg gggtagcctt ggggtatcct tggggtagcc
     2401 ttggggtagc cttggggta
//
LOCUS       DATCGS010000077         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_452099, whole genome shotgun sequence.
ACCESSION   DATCGS010000077 DATCGS010000000
VERSION     DATCGS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452099"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..141
                     /locus_tag="VIL39_01525"
     CDS             1..141
                     /locus_tag="VIL39_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5480139.1"
                     /translation="MRCARAIGAKALAVATGGARLEELKMRQPDWAIPDLRAINAREV
                     VA"
     gene            complement(438..1160)
                     /locus_tag="VIL39_01530"
     CDS             complement(438..1160)
                     /locus_tag="VIL39_01530"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEY5480140.1"
                     /translation="MKLLRLAFKRETAMPMLALTFSSAASVALVVARIVWTGRFGYSF
                     LVWNLFLAWLPMVFALLACENYQTTSGRDWRFLGLAGAWLLFFPNAPYIFTDLIHLTK
                     RFYPYFWVDLSLILLCALTGLVLGFVSLYLMQSVVERMLGRAASWLFVAAVAALTGFG
                     IYVGRFMRFNSWDVVFKPRQVYHGIGNWVADPLANSTSLAFPLLFGTFVFITHLMLYA
                     LTHLRPAQPMMMPQTTQAPAPA"
     gene            complement(1284..2702)
                     /gene="creD"
                     /locus_tag="VIL39_01535"
     CDS             complement(1284..2702)
                     /gene="creD"
                     /locus_tag="VIL39_01535"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY5480141.1"
                     /translation="MNSTEPTSYSVPPRIAVISEVRSFLKRCATIFKMAGVVLLILLL
                     LAPLTMIRSVLQERLSRRNETVANITSSWGSDQTIVGPVLIVPYRYAVRTWKEQPAAG
                     GKTERVEVVSTAVANACFLPAAMKIDGEVDPTELHRGIYRAVVYSAKLELEGEFARPD
                     FSNQRIEDKDILWEEALVAFIIPDLRGMKETLQLKWGAQSLPLLPGSKLRGFSSGIFA
                     RVAGLRESSGTIPWKLGFTLRGSGGLAFAPVGIQNSVKLASPWPDPSFRGAFLPSERK
                     VTHEGFTANWQVSYYGRNYAQQWTDQDASGALTADSAQSSAFGVSLISGIDAYRNVER
                     AIKYGVLFLALVYTAFFLFEVLSTLRIHPFQYAIVGAAFCLFYLGLLSLSEFVAFGVA
                     YLVAAVVTISLICFYSAKVLKSGQRTFIIAGLLAAIYGFLYVSLQLQDYSLLFGTAGL
                     FVVLGIVIFTTRNIDWYVRDQA"
     gene            complement(2699..>2889)
                     /locus_tag="VIL39_01540"
     CDS             complement(2699..>2889)
                     /locus_tag="VIL39_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480142.1"
                     /translation="ALYVFMADAIRVAGQGVEALRNVLPARFNWPLFGIALLLMAAPV
                     FQAYQQISNRTKASQSEL"
BASE COUNT          659 a          880 c          845 g          505 t
ORIGIN      
        1 attcggtgcg cgcgggccat tggcgcgaaa gcgttggcgg tggcgaccgg cggcgccagg
       61 ctcgaagaac tcaagatgcg ccagcccgac tgggcgatac cggatttgcg ggcgatcaac
      121 gcccgggaag tagttgcctg attcagctca gtgccggtgc tttccgccgc cacaacaacc
      181 gccgcccccg cacgaaccac cgccgcagga accgccaccg cagcatgagc gcttgccgct
      241 gctcgggttg ccgccgacgg aggaggcgaa aacagataat tgcttggaaa gcttggccga
      301 cccgcaatgc gggcatttgg tccctttcca tttgctggaa cggaccagga tttcgctgtc
      361 ctgctcgcac ttgttgcaat gaaactcgta aatcggcatg ggaacaggat agccgcccgc
      421 taagaaacct caagtcatta tgcgggcgca ggcgcttgcg tcgtctgcgg catcatcatg
      481 ggttgcgccg gccgcaaatg cgtcagcgca tacaacatga ggtgggtgat gaacacgaac
      541 gtgccgaaga gcaaaggaaa ggccagcgag gtcgaattcg ccagggggtc ggcgacccag
      601 tttccgatgc catgatagac ctgccgcggc ttaaacacca cgtcccaact gttgaagcgc
      661 ataaagcggc ccacgtaaat gccgaaacca gtcaaggccg cgacggcagc aacgaaaagc
      721 cagctcgccg cgcgcccgag cattcgctcg acgaccgatt gcatcaggta taaggagaca
      781 aagcccagca cgagtccggt cagagcgcag agcaggatca gactcaagtc cacccagaaa
      841 taaggataaa agcgcttcgt caggtgaatc aagtccgtga agatgtaggg cgcgttgggg
      901 aagaacagta accatgcccc ggccagaccc aggaaacgcc agtcgcgccc ggaagtggtc
      961 tggtaattct cacaagccag cagggcgaac accatcggca gccatgccag gaacagattc
     1021 caaacgagga aagagtagcc aaatctgccg gtccagacaa ttcgcgccac caccagcgcc
     1081 acgctggcgg cggaagagaa agtcaaagcg agcatcggca tagccgtctc gcgcttgaaa
     1141 gcgagcctta ataatttcat gaataacttg ctgtcgttcg acatactacc ataacccacc
     1201 gctcaatcta atcagtgaaa ccatccgctt tgcccggaag gccacgtgcc gccgccaagt
     1261 gtcttcatcg gcctttgggt atcttaggcc tggtcgcgca cataccagtc gatgttccgc
     1321 gtggtgaaga tgacgatccc cagcaccacg aacagcccgg ccgtcccaaa cagcaacgag
     1381 tagtcttgaa gctggagcga gacatagagg aacccataga tggcggccag cagacccgcg
     1441 atgatgaacg tccgctgccc gctcttgagc accttcgcgc tgtaaaagca gatcagcgag
     1501 atcgtgacca ctgcagcgac gagatacgcc acgccgaaag caacgaactc cgagagtgac
     1561 agcaacccga gatagaaaag gcagaacgcc gcgcccacga ttgcatactg gaacggatga
     1621 atcctgagtg tggacagcac ctcaaacaga aagaacgccg tatatactag cgcgaggaac
     1681 agcacgccgt acttgatggc gcgctcgacg ttgcggtaag cgtcgatgcc cgaaatcaag
     1741 ctcacgccaa aggccgagct ttgtgctgag tcggctgtca gcgcgccgga agcgtcctgg
     1801 tccgtccact gctgggcata gttgcgtccg tagtaggaaa cctgccagtt ggcggtgaaa
     1861 ccttcgtggg tgaccttgcg ttcggagggc aggaacgcgc cgcggaagct cggatccggc
     1921 cacggcgacg cgagcttaac gctgttttga atcccgaccg gcgcaaaggc cagtccgccg
     1981 ctgccgcgca gagtaaatcc gagtttccaa gggatggttc cgctgctttc ccgcaaaccg
     2041 gcgacccggg caaaaatgcc cgacgagaag cctctcagct tgctgcccgg cagaagcgga
     2101 agactctgtg cgccccactt cagttgcagc gtctccttca tcccgcgcaa atccgggatg
     2161 atgaaggcga cgagggcttc ctcccagaga atgtccttgt cctcaattcg ctggttgctg
     2221 aaatctggcc gcgcgaactc gccctccagt tccaactttg cgctataaac caccgcacgg
     2281 taaatccccc gatgcagttc cgtggggtcc acttccccgt ctatcttcat cgctgcgggc
     2341 aggaagcagg cattggccac ggcggtgctc actacctcca cgcgctcggt cttgccgccg
     2401 gctgccggtt gctccttcca tgtcctgacc gcgtaccgat atggcacgat caatacgggg
     2461 ccgacgatcg tctggtcgct gccccagctc gaggtgatgt tggcgacggt ctcgttccgt
     2521 cggctcagcc gttcctggag cacggagcgg atcatcgtca gtggcgccag cagcagcagg
     2581 atgagcagga cgacgcctgc catcttgaag atcgtggcgc accgtttcag gaaggaccgc
     2641 acctcggaga ttacggcaat tcgcggtggc accgagtaag aggttggttc tgtgctgttc
     2701 atagttcgct ttggcttgct ttggttcgat ttgagatttg ctggtatgcc tggaagaccg
     2761 gtgccgccat caacagcagc gcgatgccga acaacggcca gttgaaccgc gccggcagca
     2821 cattgcgcaa ggcttccacc ccctggccgg ccacccgaat cgcgtcggcc atgaaaacgt
     2881 agagcgcca
//
LOCUS       DATCGS010000078         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_6626, whole genome shotgun sequence.
ACCESSION   DATCGS010000078 DATCGS010000000
VERSION     DATCGS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6626"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VIL39_01545"
     CDS             <1..730
                     /locus_tag="VIL39_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5480143.1"
                     /translation="SEETVDCDTVVLAIGQAADLSWIGPEDNLKVTPRGTLQTDPETL
                     ATSRPDVFAGGDLAFGPRIIITAVAEGQRAGRSIAKYLTGRLPEVQHKARVTVYPTEN
                     YRMPPDYEKLPRLLPPTLSLDRRIGIAEVEQVYPEPAALVQGLRCLKCHVSPIFDGDK
                     CILCGGCADVCPENCLRLVDVLSLRGDQKLQAALVARYGRLPEAGQQAGIIKDETRCI
                     RCGLCAVRCPTGAITMERAEWVPA"
     gene            884..1348
                     /locus_tag="VIL39_01550"
     CDS             884..1348
                     /locus_tag="VIL39_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY5480144.1"
                     /translation="MTQPPDDKLQRRTFLTLTFGWCSALFGLGASLAAAGRFLVPNVL
                     YEPSRRFKGLKPEDYAEGPTFLPDLRVFLFREGKKFRAVSAVCTHLGCTVNLAGKGFH
                     CPCHGSVFNPNGAVVSGPAPAPLAWLELTLSRDGHLVIDTAQPIKADTYLVV"
     gene            1350..2627
                     /locus_tag="VIL39_01555"
     CDS             1350..2627
                     /locus_tag="VIL39_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY5480145.1"
                     /translation="MLKRLWKAFQDTRVGRSIFRVGLPASNLERAQAAISSFLLHVQP
                     AKVNRRVLKFRTTLGLGLITLYLFLILTVTGVLLMFYYVPATDYAYQNMKDLEFVVTA
                     GLVLRNMHRWAAHLMVLFVVLHMCRVFYTGAYKRPREFNWVLGVMLFLLTLSLSFTGY
                     LLPWDQLAYWAITVGTSIAGYAPVVGQKLKFMFLGGHVVGESALIRFYVLHVIGLPLA
                     AVALIAVHLWRVRKDGGLARPDDPQATQEELTPAQLPLTKTYGLMELARGTTPAVGIN
                     PEDEVFTWPHLVFREILLFMATVAVLLVLAIFWNAPLEQIANPVHPPNPAKAPWYFLG
                     LQELVAYSAFWGGVVVPALLVTALIALPYVDRKRKGVGVWFSGERKVAVTVFTICLSA
                     AVVLTVIGTAFRGPNWSFQLPWKPVHVEEHGAP"
     gene            2633..4027
                     /locus_tag="VIL39_01560"
     CDS             2633..4027
                     /locus_tag="VIL39_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5480146.1"
                     /translation="MMDTSLRKFYSWFTALGLLVGGLVIAAYYKDQFREWKTWQREYV
                     KAELDRAVTPAQRAEAARIPVEIRQFVLPELDRVDRCTSCHVAVEDPSYAERRLPLAH
                     HHNHDQHPFDKFGCTICHQGQGRATTREAAHGHVEHWDRPMLEMKYIQSACGKCHLPT
                     DVPDAPKLAQGHDVFEKAGCIGCHKLHGTGGMIGPELDKVGANRSPQWLLQHFKNPAV
                     VTPGSAMPPIKASEADLDALTLYVLSFTGEPLSAYYVSIKTIPGPAAGRRLFEEKGCL
                     GCHSVGGKGGNLGPALDEVAKRHDSAWIVAHFRNPQAITPGSVMPQFDFTEQETRALT
                     AFLLSLTDTNVVGFLKVPGAASPIERGKAVYKKYGCAGCHGADAKGGIPNRNAKTAQQ
                     VPGLLYVAEGYTKDELKKRVLDGQREIPVMDPKKPPAPLYMPAWRGKIALGELDDLVA
                     YLVSLLPKGEKVDF"
BASE COUNT          729 a         1237 c         1302 g          795 t
ORIGIN      
        1 cagcgaggaa acggtggatt gcgatacggt tgtcctggcc atcggccagg cggcggattt
       61 gtcttggatt gggccggaag ataatctgaa ggtgacgccg cgcgggacct tgcagactga
      121 tccggagaca ctggccacgt cccggccgga tgtgtttgct gggggcgatc tggcgttcgg
      181 cccaagaatc atcatcaccg cggtggccga aggccagcgt gccgggcgct ccatcgcgaa
      241 atacctgact ggccggctgc ctgaagtgca acacaaggcg cgggtgacgg tctatcccac
      301 tgagaactac cggatgccgc cggattacga gaagctgccg cggctcttgc cgcccacctt
      361 gtccttggat cgccggattg ggattgccga agtggagcag gtttacccgg agccggcggc
      421 gctggtgcag ggcctgcgtt gcctgaagtg ccatgtcagc ccgatttttg acggcgacaa
      481 gtgtattctc tgcggcggct gcgccgacgt ctgcccggag aattgcctgc ggctggtgga
      541 tgtgctgtcg ctgcgcggcg accagaagtt gcaggccgcc ctcgtggccc gctatggccg
      601 cttgcccgag gccggacagc aggcaggtat cataaaagat gagacccgtt gcatacgttg
      661 cggcctatgc gccgtgcggt gtccgacggg cgctatcacc atggaaaggg cggagtgggt
      721 gccggcgtga ataaaaatgc gttacagcgg ttaaatgggt tacatgagtt acatgagtta
      781 catggttaca aaggtcggcg gcgcggagca ctcatgcaac tcatgtaact catgcaacga
      841 tgtaacctat ttaacccatg taactaactt gggctgactt tgcatgactc aaccacccga
      901 tgataagctg cagcggcgaa cgttcctgac gttgaccttc ggctggtgtt cggcgctctt
      961 cgggctgggc gcttccctgg ccgccgcggg gcggttcctg gttcccaatg tgctctatga
     1021 gcccagccgc cggttcaagg ggctgaagcc ggaagattat gccgaaggcc cgactttcct
     1081 gcctgacctg cgggtcttcc tgttccgcga gggcaagaag tttcgcgccg tgtcggcggt
     1141 gtgcacgcac ctgggctgca ccgtcaacct cgctggcaag ggattccact gtccctgcca
     1201 cggcagtgtg ttcaacccga acggcgccgt ggtgagcgga ccggctccgg cgccgctggc
     1261 atggctcgag ctgacgctca gccgggatgg gcatttggtc atcgacactg cgcagccgat
     1321 caaggccgat acatacctgg tggtgtgaca tgctgaaacg actctggaaa gcattccaag
     1381 acacgcgcgt aggccgctcg atcttccgcg tcgggctgcc ggcgagcaac ctggagcggg
     1441 cgcaagccgc catatcgagc ttcctgttgc acgtgcagcc ggccaaggtc aaccggcgcg
     1501 tgttgaagtt tcgcacgacg ctggggctgg gactgatcac cttataccta ttcctgatcc
     1561 tgaccgtgac cggcgtgttg ttaatgttct attacgtgcc ggccaccgac tacgcctacc
     1621 agaacatgaa ggacctggag tttgtggtca ccgccggcct cgtgctgcgc aacatgcacc
     1681 gctgggccgc ccacctgatg gtgctgtttg tggtgctgca catgtgccgc gtcttttaca
     1741 ccggcgctta caaaaggccg cgggagttta actgggtgct gggcgtgatg ctgtttctat
     1801 tgacgctgtc gttgtcgttc accggttacc tgttgccgtg ggaccagctt gcctattggg
     1861 ccattacggt gggcaccagt atcgccggct acgcgccggt cgtcggccag aaactcaagt
     1921 tcatgttcct gggcggccat gtggtgggcg agtcagcgct gatccggttc tatgtgttgc
     1981 atgtcattgg gctgccgctg gccgcggtgg cgctcatcgc cgtccacctc tggcgcgtgc
     2041 gcaaggatgg cgggctggcg cggcccgatg acccgcaagc gacgcaggag gagctgaccc
     2101 ccgcgcaact gccgttgacg aagacctacg gattgatgga actggcccgg ggcaccacgc
     2161 ccgcggtagg catcaaccct gaggacgaag tgttcacctg gccgcatctg gtcttccgcg
     2221 agattctgct gttcatggcc acggtcgccg tgttgttggt gctggccatc ttctggaacg
     2281 cgccactgga gcaaatcgcc aatccggttc acccgcccaa tcccgccaag gcgccgtggt
     2341 atttcctggg cttgcaggag ttggttgcct attccgcctt ttggggcggg gtggtcgtgc
     2401 cggcgctgct ggtcacggcc ctgatcgcgc tgccctatgt tgatcgcaaa cggaagggcg
     2461 ttggagtgtg gttctccggc gaacgcaaag tagccgtcac cgtcttcacg atctgcctga
     2521 gcgcggcggt ggttttgacg gtcattggca ccgctttccg cggaccgaac tggtcatttc
     2581 aattgccttg gaaaccggtc cacgtcgagg agcacggcgc cccgtgagtt gaatgatgga
     2641 tacctcgcta cgcaagtttt attcctggtt cacggcgctc ggcctgctgg tgggcggatt
     2701 ggtcattgct gcttactaca aggaccagtt ccgcgagtgg aagacctggc aacgggagta
     2761 cgtcaaagcc gagctggacc gggcggtcac ccccgcccaa cgggccgagg cggcgcggat
     2821 tcctgtcgaa atccggcagt tcgtcctgcc cgagctggat cgcgtggacc gctgcacaag
     2881 ctgccatgtc gcggtggagg accccagtta tgccgagcgg cgcctgccgc tggcccatca
     2941 tcacaaccac gaccagcatc cgttcgacaa gtttggctgc accatctgcc accaggggca
     3001 ggggcgtgcc acgacccggg aggccgctca cggccacgtc gagcactggg atcggccaat
     3061 gctggagatg aagtacatcc agtccgcctg tggcaaatgc caccttccga ccgacgtgcc
     3121 ggacgcgccg aagctggcgc agggccacga cgtgttcgag aaggcaggct gcatcggctg
     3181 ccacaagctt catggcaccg gtggtatgat cgggccggaa ctcgacaagg tgggcgccaa
     3241 ccgcagtccc caatggctgc tgcagcactt caagaacccg gcggtcgtca cgcccggctc
     3301 ggccatgccg cccatcaagg ccagtgaggc cgacctggat gccctgaccc tgtatgtgct
     3361 gagcttcacc ggcgaaccgt taagcgcgta ctatgtctcc ataaaaacca tccccggccc
     3421 ggcggcaggc cgccggctct tcgaggagaa aggttgcctc ggctgccaca gcgtaggcgg
     3481 caaaggaggc aacctcggcc cggcgctgga cgaggtggcc aaacgccatg actccgcctg
     3541 gattgtcgcg catttcagga acccgcaagc gatcaccccg ggctcggtca tgcctcagtt
     3601 tgactttacg gaacaggaga cccgcgcgct gactgcattc ctgctctcgc tgaccgacac
     3661 caacgtggtc ggcttcctga aggttcccgg cgcggccagc ccgatcgagc gcggcaaggc
     3721 cgtctataag aaatatggct gcgccggttg tcatggcgcc gacgccaagg gcggcatccc
     3781 caatcgcaat gccaagaccg cccaacaggt gccggggctc ctgtatgtag ccgaaggcta
     3841 taccaaggac gaattgaaga agcgcgttct cgacggccag cgggaaatcc ccgtcatgga
     3901 cccgaagaaa cctccagcac cgctctatat gcctgcctgg cgcggtaaaa ttgctctggg
     3961 cgagctcgac gacctggtcg cgtacctggt cagcctgcta cccaagggcg aaaaggtgga
     4021 cttttgaccg agagttcgga aaccagtagc gcggccagtt cgg
//
LOCUS       DATCGS010000079         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_402675, whole genome shotgun sequence.
ACCESSION   DATCGS010000079 DATCGS010000000
VERSION     DATCGS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402675"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..1549)
                     /locus_tag="VIL39_01565"
     CDS             complement(389..1549)
                     /locus_tag="VIL39_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480147.1"
                     /translation="MSATRSSVGVRAMSLIELLCVMAIIMVLAALLLPALGQAKARAK
                     RIQCLDHLHQTGIGFVSFANEHNGRFPMAVPASAGGSLELAQGGYLLPGDFYFSYRHF
                     QAASNELVTPKLVVCPADTRVPATSFATLSNANLSYFIGVNAEFARPTSILAGDRNLT
                     NDYTTPGTVLRLGQNYALRWTDELHRFKGNLLFSDGHVEQKNNPALVPTGGQVPGVAN
                     LALPTVRQPGTAASSPGAGASAWTPSAPSKADAPDSGTFTSATNAKARRGLIAVSRPR
                     GSATPDRVSVTPAGGASKEAPASPRVEKRSTNAVAASTPAKPEEAEAIFSQFGLWLTA
                     VTGGLVEKSLWWFYALLLLLAVATVVLSKSVRGRKKRAAKPPMRFPWRPGPD"
     gene            complement(1664..2086)
                     /locus_tag="VIL39_01570"
     CDS             complement(1664..2086)
                     /locus_tag="VIL39_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5480148.1"
                     /translation="MHTLTIPANRTLLDLLRDHLGLLGTKCGCEIGECGACTVLLDGE
                     PVNSCLVLAPQINGREVVTVEGLAQDGKLHPLQESFLDHDAAHCGFCTPGMLMSAKAL
                     LDWNPRPTETEIRTAISGNLCRCTGYQQIVQAIAKAAR"
BASE COUNT          400 a          655 c          643 g          393 t
ORIGIN      
        1 gcgccggttg taggccattt ccgacttgtt ggtgtgcaga ttgatatgcg tatcggagac
       61 gaaagcaata cgggcaacaa tcccgtttgt cccagagtcg gcccagccgc cgatcaccgc
      121 caccatcaac accgcgagtc cgcataaaat cttcttcata acgtcatatt ataactcagc
      181 agccactcaa gagtttgctt cctgcatgtc cgtttagcgc ggcgcgcgga cattcctgtc
      241 cgcagccacg tgcttcacgc aaacgggcaa caaatccgag cctggttcgc attcgggaga
      301 ggctgcggac aggaatgtcc gcgcgccgct aaacacatac ctgccggcca cacgcacggc
      361 ggcgatagta tccggctgcg gaggagcgtc aatcaggtcc gggacgccat ggaaatcgca
      421 tgggaggttt ggctgctcgt ttctttctac cccggaccga cttgctcaga acaacagtcg
      481 ccaccgcgag gagcagcagg agggcgtaga accaccacag gctcttttct accagtccac
      541 cagttaccgc tgtgagccac agcccaaact gggagaagat ggcctctgcc tcttccggct
      601 tggccggggt gcttgcggcc actgcattgg tggatcgttt ctcgaccctc ggcgaggcgg
      661 gggcttcctt cgaagcgcca cctgcgggcg tgaccgacac tcggtccggt gtcgcgcttc
      721 cgcgaggccg actaactgca attaaacctc tgcgggcctt ggcgttggtt gccgacgtaa
      781 acgtgccaga gtcgggcgcg tccgccttgc tgggtgcgct tggtgtccac gcggaggccc
      841 cggcgccggg cgacgaagcg gcagttccag gttgccgcac ggtgggcagg gcaagattcg
      901 caacaccggg aacctggcca ccggttggga ccagagcggg attgttcttt tgctccacgt
      961 gtccatcgga gaacagcagg ttgcccttga accggtgcaa ttcatccgtc cagcgcagag
     1021 cgtagttttg gccgagtcgt agcacagtgc ccggggtggt atagtcgttc gtaagatttc
     1081 ggtcgccagc gaggatcgag gtgggccgcg cgaactcggc attcacccca atgaaatagc
     1141 tcagattcgc gttgctgagt gtggcaaagc tggtcgccgg cacgcgcgta tcggccgggc
     1201 aaaccacgag cttcggtgtg accagctcat tggaggccgc ctggaagtgc cgataggaga
     1261 agtagaaatc acccgggagg aggtaaccgc cctgggccaa ttccagcgag ccgcccgcgc
     1321 tcgcggggac agccatggga aaccggccgt tatgctcgtt ggcgaaactg acgaagccga
     1381 tgccggtctg atggagatga tcgaggcact ggatccgctt tgcccgcgcc ttggcctggc
     1441 ccagtgccgg cagcaacagc gccgccagca ccatgatgat cgccatgaca cacagcagtt
     1501 caatcaggct catcgcccgg acaccgacgc tggaccttgt agcgctcata ccctgcccat
     1561 ttcgtccagc atcgccaatg ccagacggaa tccctcaggg attcagcgtg cgggcgtcgc
     1621 catgcgcgtg cttgtcacgc gaactgcgtc accaccactc cgctcaccgg gcagcttttg
     1681 caatagcctg cacgatctgc tggtagccgg tgcagcggca aaggttgccc gagatggctg
     1741 tgcggatttc cgtttccgtt ggccgcggat tccagtccag cagggccttc gcgctcatta
     1801 acatgccggg agtgcagaag ccacagtgcg cggcatcgtg gtccagaaat gattcctgca
     1861 atgggtgcag cttgccgtcc tgcgccaacc cctccaccgt cacaacttcg cgcccgttga
     1921 tttgcggcgc aagcaccagg caggagttca ccggctcgcc atcgagcagg accgtgcatg
     1981 cgccgcactc gccgatctcg cagccgcact tcgtgcccag caggcccagg tggtcccgca
     2041 gcagatcaag cagcgtgcgg ttggcgggga tggtcaaagt gtgcaagcgc c
//
LOCUS       DATCGS010000080         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_6810, whole genome shotgun sequence.
ACCESSION   DATCGS010000080 DATCGS010000000
VERSION     DATCGS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6810"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VIL39_01575"
     CDS             <1..305
                     /locus_tag="VIL39_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480149.1"
                     /translation="LMISVLGGIVTLLLTVNPAKLFATPGLALATADFDAARKSHLLL
                     GAANLFSFWLVGVLAVGLAKLAGVPFLRAAWFVFAFWVIQESFFMFLGGVFGQFAL"
     gene            450..1649
                     /locus_tag="VIL39_01580"
     CDS             450..1649
                     /locus_tag="VIL39_01580"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY5480150.1"
                     /translation="MTQRFNSIESVVTDLQRGKMVIVVDDADRENEGDLIMAGQFVTP
                     AAVNFMAKHGRGLICVPTTSERLQQLGIERMVRQNRDTFKTDFQVSVDATHGVTTGIS
                     AGDRAETIQIMADPTAVPEDLVAPGHIFPLRARPGGVLQRAGHTEATVDLVRLADCRP
                     IGVICEILNDDGSMARLPELIKFARKHRLKICAIADLIQFRRTREKLVERIEVVKLPT
                     EYGDFDLYLYQSKLDAQHHLALVCGKVAGKRNVLVRVHSECLTGDVFGSRRCDCGPQL
                     HQAMQQVAEAGCGVVVYMRQEGRGIGLAPKIKAYKLQEQGYDTVEANQKLGFDTDLRE
                     YGMGAQILTDLGLKRIRLLTNNPRKIVGLEGYGLEVVEQIPIKVKPNPHNARYLKTKR
                     DKLGHLL"
     gene            1699..2199
                     /gene="ribH"
                     /locus_tag="VIL39_01585"
     CDS             1699..2199
                     /gene="ribH"
                     /locus_tag="VIL39_01585"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY5480151.1"
                     /translation="MLKTIKARTLRAGNARFAIVASQYNGRYVNAMLAAAKAEFKQAG
                     AKEVQVVRVPGAYEVPLVAARLARTASPHASRITHHPPPLSAIVCLGVILRGQTVHAA
                     HIGEAVSRALMEIQVRHEVPVIHEVLLLENEEQARARCLDPKRNRGREAAQTALAMAR
                     VMASLA"
     gene            2245..>3468
                     /locus_tag="VIL39_01590"
     CDS             2245..>3468
                     /locus_tag="VIL39_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY5480152.1"
                     /translation="MASNAQLTPMMAQYRRIKSELPKDALLLFRLGDFYEMFFEDAQT
                     GAQLLNVALTKRGIVPMCGIPFHAVNAYVGRLLKAGRKVAICDQMEDARPGQLVKREV
                     TQILSPGTHFDERMLKAERNNFLAAVCPAGRTFGLAFVDLTTGDFITTEVEGDTEALT
                     ELQRLRPAELIYPGEAGEVRDLLRGGRRGAAEERKSLIPSLSPSDGERGTPKPGEEGG
                     QPVAPAVTGYGWILNGYDDWVFAPETALFTVREHFKVTSLDGFGLRDRAAAIGAAGAV
                     LHYLTQHLRRDVANLTRISFYQRSDYLALDFTTLRHLEILEPLHHDAPRNASLYGALN
                     RTVTPMGARRLRDWLSQPLATVGPIQRRQEAVQTLVETSAGLEELRAQLAQVRDLERT
                     LGRLSAGTGNARDLVA"
BASE COUNT          656 a          978 c         1203 g          631 t
ORIGIN      
        1 cgctgatgat cagcgtgctg ggcggaatcg tgacgctgct gctgacggtt aacccggcca
       61 agctgttcgc cacgcccggt ttggccctgg cgactgccga cttcgacgca gcccggaaga
      121 gccacctgct gctgggcgcc gccaacctct tctccttctg gttggtcggc gtgcttgccg
      181 tcggcctcgc caagcttgct ggggtgcctt tcctccgtgc cgcctggttc gttttcgcgt
      241 tttgggtgat ccaggaatcc ttctttatgt tcctgggagg agtgttcgga caattcgcgc
      301 tctaagcggc agggagagct tgaaatctca cacgatccgc gggcgcggcg tagccgccaa
      361 ggtcaggacg tgctttgatg ttgcagccct cccagggttt cttgtgactg gcatttatcc
      421 tccgatccga tagtttgatt actggttata tgacgcaacg cttcaattcc atagaatccg
      481 tggtcaccga cttgcagcgg ggcaagatgg tgatcgtggt ggacgatgcc gaccgcgaga
      541 acgaaggcga cctgatcatg gcgggccaat tcgtgacgcc agcggcggtg aatttcatgg
      601 ccaagcacgg gcgcgggctg atctgcgtgc ccacgacgtc cgaacgcctg cagcaattgg
      661 gtattgagcg gatggtgcgg cagaaccgcg acaccttcaa aacggatttc caggtcagcg
      721 tggacgccac acacggggtc accaccggca ttagcgccgg cgaccgagcg gagacgatcc
      781 agatcatggc cgacccgacg gcggtgccgg aggacctggt ggcgccggga cacatatttc
      841 ccctgcgggc gcggccgggc ggggtgttgc aacgggcggg tcacaccgag gcaacggtgg
      901 acctggtgcg gctcgcggat tgccggccaa tcggcgtgat ctgtgagatt ttgaacgacg
      961 acggctcaat ggcgcggctg ccggagctga tcaagttcgc ccggaagcac cggctgaaga
     1021 tatgcgcgat tgccgacctg atccagttcc gccgcacacg ggagaagctg gtggagcgca
     1081 ttgaggtggt gaagctgccg acggaatacg gagactttga tttatacctc taccagtcga
     1141 agctggacgc gcagcaccat ctggcgctgg tatgcgggaa ggtggcgggg aagcgaaatg
     1201 tgctggtgcg cgtccacagc gagtgtttga cgggggatgt gttcgggtcg cgccgatgcg
     1261 attgcgggcc gcagttgcac caggcgatgc agcaggtggc cgaggcgggc tgcggcgtgg
     1321 tggtctatat gcgccaggaa gggcggggca ttggtctggc tccgaaaatc aaggcctaca
     1381 aactgcaaga gcagggctac gacaccgtgg aggccaacca gaagctcggc ttcgacacgg
     1441 acctgcgcga gtatggcatg ggggcgcaga tcctgacgga cctcggcctc aagcggattc
     1501 gcctgctgac gaacaacccg cggaagatcg tgggcttgga aggctacggg ctggaagtcg
     1561 tcgagcagat tccgatcaag gtgaagccga atccccataa tgcccgctac ctgaagacga
     1621 agcgggataa actggggcat ttgctgtagc cccagaacgg agtgttggag cagtggagta
     1681 ttggagtgct ggaacgttat gttgaagaca ataaaggcca gaacattgcg ggcgggtaat
     1741 gcgcgcttcg ccatcgtcgc ttcgcaatac aacgggcgtt acgtcaacgc catgctggcg
     1801 gcggcgaaag cggaattcaa acaagcaggg gccaaggagg tccaggtagt gcgcgtgccc
     1861 ggcgcttacg aggttccgct tgttgcagcg cgattggcaa ggactgcttc ccctcacgca
     1921 tcacgcatca cgcatcatcc cccccccctc tcggccatcg tttgcctggg agtgattttg
     1981 cgcgggcaga cggtgcatgc ggcgcacatc ggcgaagcgg tcagccgcgc gctgatggag
     2041 attcaagtgc gacatgaagt gccggtcatc cacgaggtgc tgctgctgga aaacgaggag
     2101 caggcgcgcg caaggtgcct ggacccgaag cgaaaccggg gccgggaagc ggcgcagacg
     2161 gcgctggcaa tggcgcgggt gatggccagc ctggcctaac tttcgtttcg acgagccggt
     2221 gaatcctgtc taacgtcttg ggcgatggcc agcaacgccc aactcacgcc catgatggcg
     2281 cagtatcgcc gcatcaagag cgaactgccc aaagacgcgc tgctgctgtt tcggctgggc
     2341 gacttctacg agatgttctt cgaggacgcc cagacggggg cgcaactgct caacgtggcg
     2401 ctgaccaagc gtgggatcgt gccgatgtgc ggcattcctt tccacgccgt caatgcgtac
     2461 gtcgggcgcc tgctcaaagc gggccgcaaa gtggccattt gcgaccagat ggaagacgcg
     2521 cggcccgggc aattggtgaa acgggaggtg acgcagattc tcagcccggg cacgcacttt
     2581 gatgagcgga tgctcaaggc ggagcggaac aacttcctgg cggcggtgtg tccggccggg
     2641 cgaacgttcg ggctggcctt tgtggacctg accacgggcg atttcataac caccgaagtg
     2701 gagggagaca cggaagcgct gacggagttg cagcggctgc ggccagcgga gctgatctat
     2761 cctggggaag cgggggaggt gagggatttg ttgcggggcg ggcgtcgtgg ggcggcagaa
     2821 gaacgcaagt ccctcatccc gtccctctcc ccatcggatg gggagagggg gaccccgaag
     2881 cccggggagg aagggggcca acccgttgcc ccggcagtta cgggatatgg ttggatcctg
     2941 aacggctacg acgattgggt cttcgcgccc gaaacggcgt tgttcaccgt gcgcgagcat
     3001 ttcaaagtca cctcgctgga tggctttggg ctaagggacc gggcagcggc tattggcgcc
     3061 gccggggcgg tgttgcatta cctcacgcag cacctccggc gcgatgtggc gaacctgacg
     3121 cgcatttcgt tctaccaaag aagcgactac ctggcgctgg acttcacgac actcaggcac
     3181 ctcgaaatac tggagccgtt gcatcatgac gcgccgcgga atgcgtcgct ttacggcgcg
     3241 ctcaatcgca ctgtcacacc tatgggcgcg cggcggctgc gggattggct gtctcagccg
     3301 ctcgccacgg tgggaccgat tcagcggagg caggaagcgg tgcagacgtt ggtggagact
     3361 tccgccggct tggaggaact ccgggcgcaa cttgcgcagg tgcgggatct ggagcggacg
     3421 ctcggacggc tgagcgcggg aaccgggaat gcgcgggacc tggtcgcc
//
LOCUS       DATCGS010000081         3076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_106236, whole genome shotgun sequence.
ACCESSION   DATCGS010000081 DATCGS010000000
VERSION     DATCGS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106236"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VIL39_01595"
     CDS             complement(<1..651)
                     /locus_tag="VIL39_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5480153.1"
                     /translation="MFVDEIKIYSRAGHGGKGCVAFQREKYCPKGGPSGGNGGRGGDV
                     ILEADHDLNNLIAQYYQPRLIAQDGRFGMGNGMDGHAGKDLVVRVPCGTLVWRLPQEG
                     SPETDQEAQPESSQAALRASYRRAPLLRLAARDRALEVDLSRAEGDTPAAAGAKLKGE
                     LVVDLTEHGQCFVLCKAGRGGLGNRNFATSTRQTPRFAQPGEEGDEGNFLFELRTMA"
     gene            complement(840..1103)
                     /gene="rpmA"
                     /locus_tag="VIL39_01600"
     CDS             complement(840..1103)
                     /gene="rpmA"
                     /locus_tag="VIL39_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5480154.1"
                     /translation="MAHKKGQGSVRNGRDSVSKRLGVKRYGGEVVTAGNILIRQRGSK
                     FVAGQNVGTGRDWTLFALANGKVAFDKNSHRINVVPEAAPAAS"
     gene            complement(1144..1455)
                     /gene="rplU"
                     /locus_tag="VIL39_01605"
     CDS             complement(1144..1455)
                     /gene="rplU"
                     /locus_tag="VIL39_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5480155.1"
                     /translation="MYAVLETGSKQYRVSVGDRLEVELLPVEAGQPVTFDRVLMVNND
                     GKLAVGSPTVASATVVADVVEHIRGVKTIAFKMKRRKGFHKTIGHRQELTVVKIKEIK
                     T"
     gene            1785..2552
                     /locus_tag="VIL39_01610"
     CDS             1785..2552
                     /locus_tag="VIL39_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5480156.1"
                     /translation="MKKLNPFSGPGSLRPEGQQTRREKFKVVVWTIVAANVVLFAGLL
                     IQGCRGEPATTETTGDSAAEVTSPNTNGATAAEQKPAADSSVPPTFESTPSNTNPVPN
                     TVAETASTPAPAPAQVAAKQYTVSKGDSFYKIAKDNKVSLKALTDANPGVNSAKLKVG
                     QVLQLPVGAEPSATVSTSVPAPASATASQPTVTGSKAQKHYVVKSGDTLGRIARTHGT
                     TVRAIKAANGLTSDRVVLGQNLKLPESGAARKSGTRA"
     gene            2648..>3076
                     /locus_tag="VIL39_01615"
     CDS             2648..>3076
                     /locus_tag="VIL39_01615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY5480157.1"
                     /translation="MIAQETHKACPSGLVIAREPLPEAPHSNGSFYVAQCELGRGVFA
                     RRTIREGEIILTFGGPLIDLAETKRRGALECMAFQVDPNQYIDTLAPAVFVNHSCAPN
                     TGIRNDLDLVALRDLPEGEEIRFDYSTTMDEKSFTMGCRCG"
BASE COUNT          637 a          911 c          941 g          587 t
ORIGIN      
        1 ggccatggtg cgcagctcga agagaaaatt gccctcgtcg ccctcctccc caggctgggc
       61 gaaacgcggg gtctggcgcg tcgaggtggc aaagttgcgg tttcccaggc cgccgcgtcc
      121 ggctttgcac aagacgaagc attgaccgtg ttcggtaaga tctacaacca actcgccttt
      181 cagcttcgcc ccggcagcag ccggggtatc gccttccgcg cgggagagat caacctccag
      241 cgcgcggtct ctcgcggcca ggcgtaacaa gggagcgcgg cggtaactgg cgcgcagagc
      301 agcctgtgac gactcgggct gagcctcctg gtcggtttca gggctgcctt cctggggcaa
      361 tctccacacc agcgtgccgc agggaactct aacgaccaga tccttgccgg catggccgtc
      421 catgccattg cccatgccga atcggccgtc ctgggcgatg aggcgcggct gatagtactg
      481 ggcgatgagg ttattcaggt cgtggtcggc ttcgaggatg acgtcgccac cacgcccgcc
      541 gttgccgccg ctcgggccgc ccttgggaca atacttctcg cgctggaacg caacacagcc
      601 tttgccgccg tggccggccc gcgagtagat cttgatttcg tcaacaaaca tggcgacaag
      661 cccaaaaacg gagtggtgga gtgatggagt ggcggggtga tggaacaacg gctgttcccc
      721 ccatcactcc attactccaa cactccatca ctccgttatt ccggtccgcg ttcactccgg
      781 ccttccccca ataaaaatgg cgaggccaaa gcctcgccgc gaagacttgg ccggcctgcc
      841 taactggcgg ctggagccgc ttccggcacc acattaatgc ggtgactgtt cttatcgaaa
      901 gccactttgc cgttcgccag ggcgaataac gtccaatcgc gcccggtgcc gacgttctgg
      961 ccagccacga acttgctgcc gcgctggcgg atgaggatgt tgccggcggt gacaacttcc
     1021 ccgccatatc gtttgacgcc cagccgcttg ctgacgctgt cgcgcccgtt gcggacgctg
     1081 ccttgacctt tcttgtgtgc cataaatgtc taatacgtga tacgtgatgc gtgatcgcgg
     1141 tcttcaggtc ttgatttcct tgatcttaac aaccgttagt tcctgccgat gcccgatcgt
     1201 cttgtgaaag cccttgcggc gtttcatctt gaacgcgatt gtcttcacgc cacgaatgtg
     1261 ctcgacgacg tcggctacca cggtggcgct ggccacggtg ggcgaaccca cggcgagttt
     1321 gccgtcgttg ttgaccataa ggacgcggtc aaaggtcacc ggctgcccgg cttcgacggg
     1381 cagaagttcg acttccagcc tgtcgcccac gctaacgcga tactgcttgc ttcctgtttc
     1441 taatacagcg tacataaaat atctaaacca aaaggacggg aatgaaacca gaaccgcacc
     1501 gtggtgtcaa cacgtcaatt tcgaccgcca aattatcccc cctttgacac aaaagcaagc
     1561 tcgcattgtc cgttacggta gaatgatact aaggccaggg cgagaagctt ggttgcactt
     1621 tcggccggcc ggcagtgcag gaattgcaca gcgccgccca ttgtcgtggg cggatgggga
     1681 gtcccggaat ggacgactat gtttaagtta ctatgaatca gtgggttgtc gcgtttccct
     1741 gagctgtggc actcacggtg ctaaagcaag taagccgaaa gactgtgaag aagttgaatc
     1801 catttagcgg gccgggttcg ttgaggccag aagggcaaca aacacggcgg gagaaattca
     1861 aggtcgtggt gtggactatc gtggcggcga atgtggtctt gtttgcggga ctgctgatcc
     1921 aaggctgccg cggggaaccg gcaacgaccg aaaccactgg cgacagcgcg gccgaagtga
     1981 cgtcgcccaa tacaaacggg gccacagcgg cggagcagaa gccagcggca gattcatccg
     2041 tcccgccgac ttttgagtcc acgccgtcaa ataccaaccc tgtgcccaac acggtagccg
     2101 agacagcgag tacccccgca ccggccccgg cgcaggtcgc ggccaagcag tacacagtct
     2161 cgaaaggcga ttcattctac aagatcgcca aggataacaa ggtctcgctg aaagcgctga
     2221 cggatgcgaa cccgggcgtg aatagcgcga aattgaaggt cggccaggtg ttgcagctcc
     2281 cggttggtgc cgaaccgagt gccacggtgt caacttcggt gccggcgccc gccagtgcca
     2341 cagcaagcca gccgaccgtg accgggagca aagcgcagaa gcattacgtg gttaagtccg
     2401 gggacacttt gggccggatc gcccggacgc atgggacgac ggtgcgcgcc atcaaagcag
     2461 ccaacggctt gaccagcgac cgcgtcgttc tcggccagaa tctgaagctg ccggagagcg
     2521 gggcggcgcg gaagtccggc acacgggctt gagcggcgcg tgcgggcgca ttcagggcgg
     2581 gtatcaacac tttgatcggg acgcgctggg ctctatgatc cagcaaagga agagaggact
     2641 tctggatatg attgcccagg aaacccacaa agcttgtccg tccgggctgg tcatagcgag
     2701 agagccactg cccgaagccc cccattcgaa cgggagcttc tatgtcgcac aatgtgagct
     2761 gggccgggga gtgttcgccc ggcgcaccat acgcgaagga gagatcatcc tgacctttgg
     2821 cgggccgctg atagatcttg cggagacaaa gcggagaggc gccttggagt gcatggcgtt
     2881 tcaggtggac cccaaccagt atattgatac gctggctcct gcagtcttcg tcaaccattc
     2941 gtgcgcgccg aacacaggga tacggaatga cctggacctg gtcgctctgc gcgacttgcc
     3001 ggagggagag gaaatacgct tcgattactc gactaccatg gacgagaagt cattcacgat
     3061 gggctgccgg tgcggg
//
LOCUS       DATCGS010000082         7486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_106316, whole genome shotgun sequence.
ACCESSION   DATCGS010000082 DATCGS010000000
VERSION     DATCGS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106316"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2322)
                     /locus_tag="VIL39_01620"
     CDS             complement(<1..2322)
                     /locus_tag="VIL39_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480158.1"
                     /translation="MKTGLSPKSLLPTILVLSAVLLTAGSLRAQPGALDPDFYRNSVF
                     DDVVFALAVQPDGKVLVAGAFTSVTGVPSPGVARVESDGSLDATFHPGLGAEGRDGAV
                     YALALYTNGVNAGKVVVAGSFTSFNRVARRSIARLNPDGTLDASFDPGSGVEDGLVYA
                     VGIQADGKIVVGGTFTTVRGASRSGIARFNADGTLDTSFAPGTGTHGAVVSLAVQPDG
                     KIVIGGMFFAVQGAFRNGIARLTSTGSVEPLSTFDPGRGADGGYVYAVALQTDGRIVV
                     AGDFTAMQGESRDGVARLNPDGSLDMDFAPDSGGANYVTVWAVAPVPGDRVLVAGEFD
                     GLANAECDGIALLDATGNVDPSFTNPVPDAVRFSVHHGEYVLALALQPNGSVVAGGTF
                     ELVNNLYRAALARLRSDGNVDPVFNQESGTFDAVLTSAVQPDGKPLLGGQFTLANGVS
                     RRGLARLNADGLLDASFDPGAGAVGQVFSLAVYTNGPLAGKIVIGGVFTKVNNITVSG
                     LARLDANGGVDTSFSSLVLSNDVQSLALQSDGKIVIGGSFTNVNGAVRNRVARLNSDG
                     SLDATFDPGGGAVGGPVFSVVVQIDGRLVIGGAFTNFNGIPRSGVARLDTSGGLDGSF
                     DPGSGVAGGGVHTVAVQPSGAVLVGGSFTTARGLPRAGVARFNPDGALDAGFAPGAGI
                     GNGEVRILTLQTNGQILIGGSFTNFNHIAAAGIARLNLDGSYDYGFDPGSGVAQAKAT
                     VQSLSVQPDGKVLVGGSFLKFDGADVWFLTRLLG"
     gene            complement(2639..3286)
                     /locus_tag="VIL39_01625"
     CDS             complement(2639..3286)
                     /locus_tag="VIL39_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480159.1"
                     /translation="MIGLLLAPSPAGAQVKPIRLRNGVIPAQASQPGSNQAKSALAQP
                     PVSGLFLVQFSSPPGREARDQLAAIGVDLLHYVPEDTFVARLRGVSLDRVRTAPLVQW
                     IGEYHPTYKIHPRLAAAARGALLTNQVVAVNALIAPQATPDEIAEVRSLFLTIAHESK
                     LRQGTILQGGLAPVRLNALCFAAARFTMSPGGCGVRVRRPTPSRPTLGTPSGFVV"
     gene            3438..3845
                     /locus_tag="VIL39_01630"
     CDS             3438..3845
                     /locus_tag="VIL39_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5480160.1"
                     /translation="MHMPAFPQRRILVVDDEPLVCDAIRMMLNFDGHLVETATNGKDA
                     LAMLEKGQFDLVITDFAMPGMKGDELAAAIKARSPNQPVVMITAYAEMLQASGNRLTG
                     VDLVIGKPFLLENLREAIAKVTPASSKDGLGVD"
     gene            4107..4577
                     /gene="lepB"
                     /locus_tag="VIL39_01635"
     CDS             4107..4577
                     /gene="lepB"
                     /locus_tag="VIL39_01635"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5480161.1"
                     /translation="MQCLVLAVLSVASYYLISHYFLQSVKVVGRSMMPTLYDSQYFLL
                     NRWVYGVRSPQRSDIVVLRDPSDNGFSVKRIIAAPGDSIYLRNGGVYLNGCKLDEPYL
                     APHTPTFTDSKYRAQLIVCGRDQYFLLGDNRQNSMDSRTYGPVPRGNILGPLIR"
     gene            complement(4597..6498)
                     /locus_tag="VIL39_01640"
     CDS             complement(4597..6498)
                     /locus_tag="VIL39_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5480162.1"
                     /translation="MNVTGSALLTANDLALAYGHQRLLEAVTLLVAPGEKVGLLGRNG
                     SGKTSLLRILAGHQNADAGDVARRRGLRIGYLPQEFELDGSLTVRQNIEAGAADLMEW
                     LRRYESGQGTDRELAVLLNHIDSADGWKLQARINATASALAAPPLDTLVGPLSGGEKR
                     RVALCRALAAQPDLLLLDEPTNHLDAESILWLEDFLRGFPGAVIFVTHDRYFLDVIAT
                     RIIELADGRCFSHPGNYTAYLESKAIRQQIAEQSERRRQRFLREELDWVRAGVRAQRS
                     KPRHRLESFYQVKGLEAPPEEREMDLLIPPPPALGNHAIQLAHAGAQVGEDDRQRWLF
                     RGLTLSLNPAQCTGIVGRNGVGKTTLLRICLGERPLEEGTLKIGKRVVFNYIDQARMQ
                     LDGTGTVLEEIAGGNETVLFGEQRLSARSYLRRFLFSDERVNEPVAQLSGGERARLML
                     AKVLRRGGNVLVLDEPTNDLDLPSLRMLEEALADFDGTVLVVSHDRYFLDRICDQVVA
                     FEDGGLFVQPGNYSYYLEKKKERDALNQSAWEGLARTAPRKPEPASAPKPARRGKLTF
                     KEQRELEGMEAAILAAETRVQELETTLNDPDFHATRSREAHGLIADLEAAKTEVTRLY
                     ERWQELAAR"
     gene            6804..7388
                     /locus_tag="VIL39_01645"
     CDS             6804..7388
                     /locus_tag="VIL39_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480163.1"
                     /translation="MRNSLVTALVVVVTAGAASAQMAGSSASDTNALRSIPATTNEVI
                     SAGSLTNSVVAGPGVDEPGSFGLDRMMDKPSVLSSQLWRSDVMAVDGGADRSFVDLDG
                     IQVQRDALFQKLDLLVRAEVRSSPVFFGVDSEVKFVEHHNYQPGIPAPVGISYRRGKQ
                     RFSFFAELAPIIEFSPSTSLEWGGGLGIRFYFNR"
BASE COUNT         1435 a         2323 c         2327 g         1401 t
ORIGIN      
        1 gcccaagagc ctcgtgagaa accaaacgtc cgcgccatcg aacttgagga acgatccgcc
       61 cacgagcacc tttccgtccg gctgcactga cagggattgc accgtggcct tggcctgggc
      121 caccccgctg ccggggtcga agccgtaatc gtaactgcca tcgaggttca agcgggcaat
      181 cccggcagcg gcgatgtggt tgaagttcgt gaagctgccg ccgatcagaa tctggccatt
      241 agtttgcagt gtcagaatcc gaacctcccc gttgccgatg cccgcgcccg gagcgaagcc
      301 ggcgtccaag gcgccgtccg ggttgaagcg cgcgacaccg gcacgcggca gaccccgcgc
      361 cgtcgtaaag ctcccgccga cgagcaccgc accgctcggc tgaaccgcta ccgtatgcac
      421 cccacccccg gcgacgccgc ttcccggatc gaaactccca tccaagcccc cggatgtgtc
      481 gagccgtgcg accccgcttc ggggaattcc gttgaaattg gtgaacgcgc cgccgatgac
      541 caggcgacca tctatctgca ccacaactga aaacacggga ccgccgacgg ccccgccgcc
      601 cggatcgaaa gtggcatcca ggctgccatc gctgttgagc cgcgccaccc tgttgcggac
      661 tgcgccgttg acgttcgtga acgagccgcc gatcacgatc ttgccatcgc tttggagcgc
      721 caaggactgc acgtcgttgc tcagcacgag cgagctgaaa ctcgtatcaa cgccgccgtt
      781 ggcatccagc cgcgccaagc cgctaacggt gatgttattc accttcgtga aaacgccccc
      841 gatcacgatc tttcccgcca gcggcccgtt ggtgtaaacg gcaagcgaga agacctggcc
      901 cacggccccg gcccccgggt cgaagctggc gtcgagcaga ccgtctgcgt tgagccgggc
      961 gagcccgcga cgcgaaacgc cgttggcgag ggtgaattgc ccgccgagca gcggtttccc
     1021 gtccggctgg acggcgctcg tgagcacggc atcgaaggta ccggactcct gattgaacac
     1081 cggatccacg ttgccgtcac tccgcaagcg agccagcgcc gcgcggtaga gattgttcac
     1141 caactcgaag gtgccacccg ccacgacgct gccgttgggc tgcagcgcca gcgcgagcac
     1201 atactccccg tgatggacgc tgaagcgcac cgcgtccggg acaggattgg tgaacgaagg
     1261 gtccacgttg ccggtggcgt caagcagcgc gatgccgtcg cactccgcgt tggcaaggcc
     1321 gtcgaattcg cccgcgacga gaactctatc gccggggacc ggtgcgacgg cccaaacggt
     1381 gacgtaattt gctccgccgg agtccggcgc gaaatccatg tccagactgc catcgggatt
     1441 cagccgggca accccgtcgc gcgattcccc ttgcatcgcg gtgaaatcgc ccgccaccac
     1501 gatgcgtcca tcggtctgca gggcgacggc atagacgtag ccaccgtcgg cgccgcgacc
     1561 ggggtcgaac gtgctcagcg gctccacgga acccgtgctg gtgagccgtg cgattccgtt
     1621 ccggaaagcg ccttggaccg cgaagaacat tccgccgatc acgatcttgc catcgggctg
     1681 gactgcgagg gacaccacgg caccatgagt ccctgtgccg ggcgcgaaac tcgtgtcgag
     1741 cgtgccgtcg gcattgaacc gcgcgatgcc ggagcggctc gccccgcgca ccgtggtgaa
     1801 ggtcccgccc accacgatct ttccgtcggc ttggattccc acggcataaa cgaggccgtc
     1861 ctcgaccccc gagccggggt cgaagctggc gtccagcgtg ccgtccggat tcaaccgcgc
     1921 gatgctccgg cgtgccacgc ggttgaagct ggtgaacgag ccggcgacga cgaccttgcc
     1981 cgcgttgacg ccattcgtgt aaagcgccag ggcgtaaacg gctccgtcgc ggccttccgc
     2041 gcccagcccc ggatgaaacg tcgcatccag gctcccgtca ctctccaccc gcgcgacacc
     2101 ggggctcgga actcccgtca cgcttgtgaa cgctcctgcc acgaggacct tgccgtccgg
     2161 ttgcaccgcc agcgcgaaca cgacgtcatc gaaaaccgag ttgcggtaga aatccggatc
     2221 caaagcgccg ggctgagccc gcagggagcc ggcggtgagg aggaccgcgc ttaagaccag
     2281 gatggtgggc aggagtgatt tgggactgag gcctgttttc acgttgcgtc cgctatacgc
     2341 tcaatcggcc gctgtctcaa gttctaaatc cagttttaaa tccggggccg tggagaaaag
     2401 gactgatgga ttcgcacggg cgagcttgcc tgagcgtgag ctgcctgcca gcctttgccc
     2461 agccgtatcg gcccgtcaac accccgacaa ttgccgccgt ctttaatggg ttggccacgc
     2521 cgcaggtggg ccgttggagt gaactcatcc ggggttactg gcggcggttt gaaacgcatc
     2581 tgccggtgat actgcgcccg gtgctggccg cgcgaaaagg ccagaatcta gaaccccctc
     2641 acacaacgaa gcccgagggc gtccccagcg ttggtcggga cggcgtaggc cgcctgacac
     2701 gcacaccgca accaccaggc gacattgtaa aacgagctgc cgcgaaacac agcgcattca
     2761 ggcggacggg cgccaaaccg ccttgcagaa tggtgccctg gcgcaacttg ctttcgtgcg
     2821 cgatggtcaa gaacagggaa cgcacctcgg caatctcatc cggcgtggct tggggtgcaa
     2881 tcaacgcatt taccgccaca acctggttgg ttaggagcgc cccgcgcgcg gcggcggcca
     2941 gtcgtggatg aattttgtag gtcgggtggt attcgccgat ccactgcacc aggggcgctg
     3001 tccgcacccg gtcgaggcta acgccacgga ggcgggctac gaaggtatct tcaggtacgt
     3061 aatgcagcag gtccactccg atagccgcga gttgatcccg cgcttcccgc ccgggcgggc
     3121 tgctaaactg gaccaggaaa agtcccgaca cgggcggctg ggcgagggcc gacttggctt
     3181 ggttgctgcc tggttgtgag gcctgggcgg ggatgacccc attgcgcaga cggatcggtt
     3241 tcacttgcgc tccggcgggg gagggcgcca gcagcaagcc gatcacaatc agaccgagag
     3301 cacttcgcga aaaaagccgg ggcttcacgc tgcaactcta atcgaaacca ggactcaaga
     3361 caagctcgtg agtggtaatg tcgtgccata tttggcttga aaacgggcca gcatcagtta
     3421 tgtatgggtt tacgccaatg catatgccag cctttccgca aagacgcatc ctggtggtgg
     3481 atgacgaacc gctcgtctgc gacgccatca ggatgatgct gaactttgac ggtcatctgg
     3541 tggagacggc caccaacgga aaggacgcgc tggcgatgtt ggaaaagggc caattcgatc
     3601 tggtcatcac cgattttgcg atgccgggga tgaaagggga cgagttggca gcggccatca
     3661 aggcgcgctc accgaaccaa ccggtggtca tgattaccgc ctacgccgag atgcttcagg
     3721 cttcaggcaa taggctcacc ggcgtggatt tggtcatcgg caaacccttt ctgctggaga
     3781 acctgcgcga ggcgattgcc aaggttactc cggcgtcgtc caaggatggc ctgggagtgg
     3841 attagatcgc caggagcaat cgcggaaacg agttcttggc accagtgatt cggcctcagg
     3901 cgacgccgca gtgtacccgt acttgtccgg taggcccatg cgtattttcc acgacttgtc
     3961 gaatcgtcac gggggtctat tatgaggagg tattaatgag tgcggcaacg caaagaacag
     4021 acgcaaggtc aatttcggcc aacccatccc ggttctctct tgcaggcaca gtcaaggcag
     4081 tatttcgtcg gattctccgg caaggtttgc agtgtctggt cctggcggtc ctgtcggtgg
     4141 ccagctacta cctcatcagt cactatttcc tgcaatcggt gaaggttgtc ggccggagta
     4201 tgatgcccac cctttacgac tcgcagtact ttctcctgaa ccgatgggtc tatggtgtgc
     4261 gctcgccgca acgcagcgac atcgtggttc ttcgcgaccc gagcgacaac gggttctcgg
     4321 tcaagcgcat cattgccgcg ccgggggact cgatctattt gagaaacgga ggcgtgtacc
     4381 tgaatgggtg caaactggac gagccgtatc tggccccgca cacgccaacg ttcaccgatt
     4441 caaagtatcg cgcccagttg atcgtgtgtg gcagggacca gtacttcctc ctgggcgaca
     4501 accgacagaa ctccatggac agcaggactt acgggccagt tccgcgcggc aatatattgg
     4561 gaccgctcat tcgctgatgg gcttctttgg ggcagatcac cgagccgcca attcctgcca
     4621 gcgctcgtat aatcgcgtca cttcggtctt cgcggcttcc aaatccgcga tcaacccgtg
     4681 tgcttctcgt gagcgggtag cgtggaagtc aggatcgttc aaggtcgtct ccagttcttg
     4741 gacgcgggtt tctgcggcca aaatggcggc ttccatccct tccagttcgc gctgctcttt
     4801 gaaggttagc ttcccgcgac gggctggttt gggggcgctg gcgggctcgg gctttctggg
     4861 tgcggttctg gcgaggcctt cccaggcaga ctggttgaga gcatcccgct ctttcttctt
     4921 ctcgaggtag taggaatagt tgcctggttg gacgaagagg ccgccgtcct cgaaggcgac
     4981 gacttgatcg cagatgcggt ccaggaagta gcggtcgtgg ctgacgacca gcacagtgcc
     5041 gtcgaagtcc gccagcgcct cctccagcat gcgcaggctg ggcaaatcca aatcgttggt
     5101 cggttcatcc aggaccagca cgttgccgcc gcgccggagc actttggcca gcatcagccg
     5161 cgcgcgctca ccgccagaga gctgggcgac cggctcgttc acgcgctcat ccgagaacag
     5221 aaaccggcgc agataagagc gcgcgctcaa ccgttgttca ccaaacagga ccgtttcgtt
     5281 cccgcccgct atttcctcca ggaccgtgcc ggtgccgtcg agctgcatcc gggcctggtc
     5341 aatgtagttg aacaccaccc gcttcccgat tttgagcgtg ccttcctcga gtgggcgttc
     5401 gcccaggcag atgcgcaaca gcgtggtttt gccgacgccg ttgcggccaa cgatacccgt
     5461 gcattgggcc gggttgagtg agagagtcag cccgcgaaag agccaccgtt ggcgatcgtc
     5521 ttcaccgacc tgcgctcccg cgtgggccaa ctggatggcg tggttcccca aggctggcgg
     5581 cggtggaatg agcagatcca tttcccgctc ctcgggtggg gcttcgaggc ccttgacctg
     5641 gtagaaggat tcgaggcggt gccgcggctt ggaacgttgc gcccggacgc ccgcgcggac
     5701 ccaatccagt tcctcgcgca ggaaccgttg ccgccgtcgc tcggactgtt cggctatctg
     5761 ctgccggatg gccttggatt ccaggtaggc ggtgtaattg cctgggtgcg aaaagcaccg
     5821 gccatcggcc agttcaatga tccgtgtggc gatgacatcc agaaagtagc ggtcgtgcgt
     5881 gacgaagatg acggcgccgg ggaacccgcg caggaaatcc tccagccata gaatggattc
     5941 ggcgtccagg tggttggtgg gttcgtccag gagcagcaaa tccggctggg ccgccaaagc
     6001 gcggcagagg gccacccgcc gcttttcgcc tccggagagc gggccgacca gagtgtccag
     6061 cgggggcgcg gccagggcgg aagcggtggc attgatgcgg gcctgcaact tccagccgtc
     6121 cgcgctgtca atgtggttca gcaagaccgc cagctcgcgg tctgtgcctt gtccggattc
     6181 ataacgccgc aaccactcca tgaggtcggc cgcgcctgcc tcgatgttct gccgcaccgt
     6241 gaggctgcca tccagctcga actcctgcgg cagatagccg atgcgcaagc cacgccgccg
     6301 cgccacgtcg ccggcgtccg cgttttgatg gccggccagg atgcgcagca ggctggtttt
     6361 gccggagccg tttctgccca gcagcccgac cttctcgccc ggggcaacca agagcgtaac
     6421 ggcttcgagc aatcgctggt ggccgtaagc cagagcgaga tcgttggcgg tcaatagagc
     6481 cgagccggta acattcatgg gcgcaaaaaa tgggaagata gcgtgctaaa gccctggagc
     6541 acagcaaaag acacgcaaaa gacacgcggt tagcgtcgtg agaacaaaag catcaggatg
     6601 agtgcgccca agcccgcagc catgcatttt gcatggcgcc ctctcgggtt cattgcattt
     6661 tgcggcaaca gttccgtggc gatcgagctc gggtttccaa gagaattgcc gatagacgcg
     6721 aggagggccc cattttaggt ctcggtacaa gccctgctcc tgattattgt acagccagta
     6781 attcgtaaaa agaaagctat agtatgcgta acagcctagt aacggcattg gtagtagtgg
     6841 tcaccgctgg cgcggccagc gcacaaatgg ccggctcatc cgcgagtgac acaaatgcgc
     6901 tccgatccat tccagccacg acgaacgagg taatttcagc cggcagcctc acgaattcag
     6961 tcgtggccgg tccaggcgtt gacgaaccag gctctttcgg cctggatcgc atgatggaca
     7021 aaccttccgt cctgagcagt cagctttggc ggtcggacgt aatggccgtt gatgggggag
     7081 cggaccggtc gttcgtggat ctggacggca tccaggtcca aagggacgct ctgttccaaa
     7141 agttggacct tttggtgcga gccgaagttc gcagctcgcc cgtcttcttt ggcgttgact
     7201 ctgaggttaa attcgtcgag caccacaact accagccggg aatccccgca ccagtcggca
     7261 tctcctatcg gaggggaaag cagcggtttt cgttcttcgc cgaactcgcg ccgattattg
     7321 agttctcccc cagcacttcc ctggagtggg gtggagggct cgggatcaga ttttacttca
     7381 accggtagga aggtgcgctg gggcctggca cagaaggcct gaccgttctg agactttcga
     7441 ctttcgactt tagactgcat ccgttagtat tcgcccatgc gtgaac
//
LOCUS       DATCGS010000083         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_700254, whole genome shotgun sequence.
ACCESSION   DATCGS010000083 DATCGS010000000
VERSION     DATCGS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700254"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VIL39_01650"
     CDS             <1..135
                     /locus_tag="VIL39_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5480164.1"
                     /translation="QVASAVEAIVKAAKTGKIGDGKVFVSSIENAIRIRTEETGEQAV
                     "
     gene            556..1917
                     /gene="dnaA"
                     /locus_tag="VIL39_01655"
     CDS             556..1917
                     /gene="dnaA"
                     /locus_tag="VIL39_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY5480165.1"
                     /translation="MQASAEKIWSAAQEQLHSMLSADIYNLWFAPLRASSQENNSITL
                     EVANDFCEVWLKDNYMGLLQDVIALASGRQMQVKFKVGAGGAPAALGPPPVQGKKKAV
                     EPAPERAVANHDLVFNPKNTFDTFVVGNNNNFACAAALAVSQAPGKSYNPLFLYGGVG
                     LGKTHLLHAIGQHVVSHKKGARVAFVTSEKFTNEYIDGIQNNQLARFRKKYRQTDVLL
                     IDDIQFLAGKERIQEEFFHTFNALHEAHKQIVLTCDRPASEIQNLEHRLVSRFEWGLV
                     TDLQPPDVEMRLAILQKKAQIMGVELPAEIMEFLANRIRANIRRLEGALIRVASYAAL
                     TGKKLSLEIVETLLREVLHEEGRYAINIEVIQKKVAEHYDIRLADMTSKRRPENIAFP
                     RQVAMFLSRQMTDTSLSAIGEAFGGRDHGTVLHACRLVKDRMEVDPNVRQVVSYLEKQ
                     LTR"
     gene            2020..3003
                     /locus_tag="VIL39_01660"
     CDS             2020..3003
                     /locus_tag="VIL39_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5480166.1"
                     /translation="MTTPVIEVSGLTKTFRTYKKQPGFGGAVRGLFRRKYEQTIAVKD
                     VGFTIQPGELVGFLGPNGAGKTTTLKMLAGLLYPASGSARVLGYVPWERDDGYRRQFA
                     LLLGQKNQLWWDLPARESMELNAKIYGIPRAALERTVAEMSELLAVRDKLDVSVRELS
                     LGERTKMELIASLLHQPKVLFLDEPTIGLDVVSQKIVREFLREHNAKQKTTILLTSHY
                     MTDIQELCERVIIIDHGTIFFDGRLSDVVDRFADSKLITIQCEGAATSSGERLGKYGE
                     VVAQSPGSIQLKVKRDRVIPVCKALLDELPVTDIDIQEVPIEEVIRRIFAR"
     gene            3224..>4418
                     /locus_tag="VIL39_01665"
     CDS             3224..>4418
                     /locus_tag="VIL39_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HEY5480167.1"
                     /translation="MKTIKSLAPKQVYVMSSGDLRLSANLQCWPEQAKMEASLAQALK
                     AEGWTVVRAHPFDKQKKHGFIDSQKMGLEVFRQLDPNAPLIVAESVWEYTHHVLPGLT
                     THRGPILTVANWSGTWPGLVGMLNINGSLTKAGVSYSTLWSEDFKDNFFKNGLRQWLQ
                     EGVVTHDQSHVKDLRLLKLPLADEQLGKCAARRFRQQKAIMGIFDEGCMGMFNAIIPD
                     DLLHQTGVFKERLSQSTLYAAMRQVTDAEARAVLDWLLKKGMTFNWGKNPETELTEAQ
                     TLEQCKMYIAAVRLADEFGCAVIGIQYQQGLKDLVAASDLAEGLLNNVDRPPVKAAKS
                     NRVLFPGEAVPHFNEVDECAGLDGLLTYRLWRELGFAPENTLHDIRWGQHYRDNGVND
                     FVWVFL"
BASE COUNT          974 a         1257 c         1324 g          863 t
ORIGIN      
        1 caggtcgcgt cggcggtgga ggcgatcgtc aaggcggcca agactggcaa gatcggcgac
       61 gggaaagttt tcgtcagctc catcgagaac gcgatccgga ttcggactga agagacaggc
      121 gagcaagcgg tttgagcgtt ccaagggctg aattgcgaga cgcctatttc agccggatag
      181 agtgccaaag ggcctgattt tattggtgga aatgggcctg tgaataactc gttaataaga
      241 gttaataggt tgtcctcgct accgggggcg acttcaccta aaaacatcgg ttgcggtggc
      301 agtcttgccc cgacgaattg ggctgcgaag agattgtgcg gggtgcacgc cttggttatc
      361 gttgtaagtt atttattcgc agcatgttgt attatatttt gaattgaagc agaatggaag
      421 ccttagtatg aaacgtcgat tttattggca gcgagctgga ttgaggatgt ggggaaccgc
      481 cgaggcgtgc gtttagggtg cggcaagtgg ctaagtgaat aaaatgcccg gaaatcgggc
      541 ttttgggcaa aagacatgca agcttccgca gaaaaaatct ggagtgcggc acaggagcaa
      601 cttcattcca tgctcagtgc tgacatctat aacctttggt ttgcgccgtt gcgcgcatct
      661 tctcaggaaa acaacagcat taccctggaa gtggccaatg acttctgtga agtctggctc
      721 aaagacaatt atatggggct gttgcaggac gtgatcgcgc tcgcgtccgg gcggcaaatg
      781 caggtgaagt tcaaagtagg cgctggcggc gcgcctgccg cgctggggcc gccgccggtc
      841 caaggcaaga agaaggcggt cgagcctgcg cccgagcgag cggtggccaa ccacgacttg
      901 gtttttaacc caaagaacac gtttgacacc ttcgtggtcg gcaacaataa caattttgcc
      961 tgcgccgcgg cgctggcggt gtcgcaggcg cctggtaaat cgtacaaccc gctgttcctt
     1021 tatggcgggg tcggtctggg caagacccat ttgctgcacg ccattggaca acatgtcgtg
     1081 tcgcacaaga agggcgcgcg ggttgcgttc gttacgtcgg agaagtttac caacgagtat
     1141 attgacggca tccagaacaa ccaactcgcc cgcttccgca agaaatatcg gcagacagac
     1201 gtgctgctca ttgacgacat ccagtttctg gccggcaaag agcggattca ggaggaattc
     1261 tttcacacct tcaatgcgct gcacgaggcc cacaaacaaa tcgtgctgac ttgcgaccgg
     1321 cctgccagcg agattcagaa cctcgagcat cggctggttt cgcggttcga gtgggggctg
     1381 gtcacggacc tgcagccgcc ggatgtcgag atgcgcctgg cgattctgca gaagaaggcg
     1441 cagatcatgg gggttgaact tccggcagag atcatggagt tccttgccaa ccggattcgc
     1501 gccaacatcc ggcgcttgga aggcgcgttg attcgggtgg cctcctacgc cgcgctgacc
     1561 ggcaagaagt tgagcttgga aatagtggag accctgctgc gcgaggtgct ccacgaggaa
     1621 ggccgctacg cgatcaacat cgaggtgatt cagaagaaag tagccgagca ttacgatatt
     1681 cggctggccg atatgacgag caaacgccgc cccgagaaca ttgctttccc gcgccaggtg
     1741 gccatgttcc tctcgcgcca gatgacagat acctcgttga gcgcgatcgg cgaagccttt
     1801 ggcggccgtg accacgggac cgtgctgcat gcctgccggt tggtcaaaga ccgcatggaa
     1861 gtggatccca acgtccggca ggtcgttagc tacctggaaa agcagcttac ccgttgagcg
     1921 tcccgtgagg ggcggcatca ttcgaacctg gggagggctg cgaagcacaa gtcgcgttcg
     1981 tccacaagac ctcacgttct gccaagcaca gcacatctca tgaccacgcc tgtcatcgaa
     2041 gtgagcggtc tgaccaagac tttccgcacc tacaagaagc aaccaggttt tggcggcgca
     2101 gtccgggggc tgttccgccg caaatacgag cagacgatag cggtcaagga cgtcggcttt
     2161 acgattcagc ccggggaatt ggtcggtttt cttggcccca acggggcggg caagaccacg
     2221 acccttaaaa tgctggcggg actgctttat cccgccagcg gttccgcccg cgtgctgggt
     2281 tatgtcccgt gggaacggga tgacggctac cgccggcagt tcgcgctgct gcttggccag
     2341 aagaaccagc tttggtggga tctgccggcg cgggagtcga tggaactaaa tgccaagatc
     2401 tacggcatcc ctcgcgccgc gctggagcgc acggtcgctg aaatgagcga actgctcgcg
     2461 gtgcgcgaca agctcgacgt cagcgtgcgc gaactctcgc tgggcgagcg cacgaagatg
     2521 gagctgatcg cgtcgctgct gcaccagcca aaagtcctct ttctggacga gccgaccatt
     2581 ggccttgatg tcgtttccca gaaaatcgtc cgcgagttcc tgcgggagca caatgcgaag
     2641 cagaagacga ccattctgct caccagccat tacatgaccg acatccagga gctgtgcgaa
     2701 cgggtcatca tcattgatca tggcacgatc ttcttcgatg gccgcctcag cgatgtcgtg
     2761 gaccgttttg ccgattccaa actcatcact atccaatgtg aaggcgcggc cacttcttcc
     2821 ggcgagcgct tgggcaaata cggcgaagtc gtggcgcaga gcccgggcag catccaactc
     2881 aaggtcaagc gtgaccgcgt gattcccgtc tgcaaagccc tgctggacga actccccgtt
     2941 accgacattg acattcagga agtccccatc gaggaagtca ttcgccggat ctttgcgcgc
     3001 tgagaggtca gggtggaacg gccacctccc ggttctctct gtcggcaacc cattcacaac
     3061 ttcattcaca ttcacagagc ctgctggcaa cacccgcccg agttgcatgt atcggctgca
     3121 aggacggtag ggcagtagtg caattccccg caaaaagatt cagtgacgcc aggccggtgc
     3181 cgtgctagaa ctttcgccag agcttttaca caatgactag aatatgaaga ccataaaatc
     3241 actcgcaccc aagcaggttt acgtgatgtc cagcggcgat ctgcgccttt cggccaatct
     3301 gcaatgctgg cccgagcagg cgaagatgga agccagcctc gcccaagccc tgaaagccga
     3361 gggctggacc gtcgtgcggg cgcatccgtt cgacaaacaa aagaagcacg gcttcattga
     3421 ctcgcagaag atgggcctcg aggtgttccg ccaactcgac ccgaacgccc cgctcattgt
     3481 agccgagtcc gtctgggaat acacccatca cgttttgccg ggcttaacca cccatcgcgg
     3541 cccgatcctt acggtcgcca attggagcgg cacctggccc ggcctggtcg gcatgttgaa
     3601 cataaatggg tcgctgacca aagccggcgt cagctacagc acgttgtgga gcgaagactt
     3661 caaggacaac tttttcaaga atggcctccg ccaatggctc caggaaggcg tggtcaccca
     3721 cgaccagagc cacgtgaagg acctgcggct gctcaagctg ccgctcgccg atgagcagct
     3781 cggcaagtgc gccgcccgcc ggttccgcca gcaaaaggcc atcatgggca ttttcgacga
     3841 aggctgcatg ggcatgttca acgcgatcat tccggacgac ctgctccacc agaccggcgt
     3901 gttcaaggaa cggctcagcc agtccaccct ttacgcggcc atgcggcagg tcactgacgc
     3961 cgaagcgcgc gcggtcctgg actggctgct caagaaaggc atgaccttca actggggcaa
     4021 gaacccggag accgaactca ccgaagccca aaccctcgag cagtgcaaga tgtatatcgc
     4081 cgccgtcagg ctggcggacg aattcggctg cgccgtcatc ggcattcaat accaacaggg
     4141 actcaaggac ctcgtggcag ccagcgacct ggccgagggc ctgttgaaca acgtggaccg
     4201 gccgccggtg aaggccgcca agtcaaaccg cgtcttgttc cccggcgaag cggtgccgca
     4261 cttcaacgaa gtggatgaat gcgccggcct ggatggtctg ctgacctatc gcctgtggcg
     4321 cgagctggga tttgcgcccg agaacaccct gcacgatatc cgctggggcc agcactaccg
     4381 ggacaacggg gtgaatgatt ttgtctgggt cttcctga
//
LOCUS       DATCGS010000084         3870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_56952, whole genome shotgun sequence.
ACCESSION   DATCGS010000084 DATCGS010000000
VERSION     DATCGS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56952"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VIL39_01670"
     CDS             <1..568
                     /locus_tag="VIL39_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480168.1"
                     /translation="CVGQYSSGEEALVGLPAAAPDVVLMDINLPGMNGIECVRQIKLA
                     HPEIEFVMLTVYEDTESVFNALAAGASGYLLKLATRQELLEAIQRVHTGGSPMTSHIA
                     RKVVQSFRHPAPMETETTQLSPREQQVLEYLAKGFLYKEISTTLGISYDTVHNHIRHI
                     YEKLHVRSRTQAVTKYLDSVHPPATAIP"
     gene            complement(687..794)
                     /locus_tag="VIL39_01675"
                     /pseudo
     CDS             complement(687..794)
                     /locus_tag="VIL39_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
     gene            complement(845..2110)
                     /locus_tag="VIL39_01680"
     CDS             complement(845..2110)
                     /locus_tag="VIL39_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480169.1"
                     /translation="MNSLHLTGALVGVLFAAYPSCPGADAPAAAPWKVGTPIATYWAG
                     PPMTDATAAQMAEGGFNLVWCTEKELDVVQRHGLRGQLTDGLLSPTTLEDSAQREKLD
                     ALIARVRHHPALYDYFITDEPSATNFSALGKLVAYLRERDPAHLAYINLFPTYASNDQ
                     LGTKGDTVTAYREHLRQFVDVVKPGLLSYDHYQFSVKGDNDGYFLNLAMIRRAAQDAG
                     LPFLNIVQAATWSPSMRVPTGDEVRFLDYTTLAYGAQGISYYVYCCAGHTGAVAHADG
                     TVTPLYYTLKTLNREFVSIAQQLQPLCSLGIYHAGMLPQGTEPVPANGRFQLDPPVPA
                     MSYTNLQPVKGVLLGYFGPAGKGKRAPTPTHVMVVNLDYQAEAVVGIRGPSRLELFDP
                     AMGRWSRATGKRADLRLSRGGGCLMRVRR"
     gene            2229..2915
                     /locus_tag="VIL39_01685"
     CDS             2229..2915
                     /locus_tag="VIL39_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480170.1"
                     /translation="MHAQRKAELRSPKQDRPGFTLIELLVVIAIIATLAALLLPALSK
                     AKQKAQATRCVSQLRQSCVAMQLYLPDYSDLLFWGDPRSPTISTEGMDWFVWAGRTNN
                     NFYTGQQNLFNRIDRPLNHYGLNYAVVTCPLDQGRTESQPHKNMEWVGNSYLFNCFGL
                     PPGTTGGLDGQRATAITQPARTVLFADCILALPTEPYGWHAIKPAGNVAAMDGHIEPH
                     TAINVTNMFW"
     gene            complement(2956..3177)
                     /locus_tag="VIL39_01690"
     CDS             complement(2956..3177)
                     /locus_tag="VIL39_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480171.1"
                     /translation="MNVFRIPGPVFNLLFGLANDAFDYILTREDFDSFKRYAYILRTS
                     LGERTGDILVTEALKLVDESPRPEPLAAR"
     gene            3604..>3870
                     /locus_tag="VIL39_01695"
     CDS             3604..>3870
                     /locus_tag="VIL39_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480172.1"
                     /translation="MASQPHPSPLVAVDSNVPLDLADGNEAVLDALNTIRGRLSNARF
                     VVTPSVFQELVHVALEDSVAARRALGQSALRQLKEWQFDLLEIVP"
BASE COUNT          802 a         1185 c         1139 g          744 t
ORIGIN      
        1 ctgcgtcggt caatactcct ccggggaaga ggccctggtc gggttgcctg ccgccgcgcc
       61 tgacgtcgtg ctcatggaca ttaacctgcc gggcatgaat ggcatcgagt gcgtgcgcca
      121 gatcaagctg gctcatcctg aaattgagtt tgtcatgctg acagtctatg aggacaccga
      181 gagcgtgttt aacgccctcg ccgccggggc cagcggttac ttgctcaaac tggcaacgcg
      241 gcaggaactg ctcgaagcca tccagcgagt gcataccggc ggctcaccca tgaccagcca
      301 tattgcccgc aaggtggtgc aatcctttcg ccatccggca ccgatggaga ccgagaccac
      361 ccagctctcc ccgcgcgaac agcaggtcct ggaataccta gcgaagggat tcctctacaa
      421 ggaaatctcc accactctgg gcatcagcta cgacaccgtc cacaaccaca tccgccatat
      481 ctacgagaag ctgcatgtcc gttctcgcac ccaggccgtg accaagtacc tcgactccgt
      541 gcatccgccc gcaacggcaa ttccgtagcg cagcgggggt catctgcgcc gctcagttat
      601 gcgcagaaaa cacaaccttg gattcctgcc cgggtccacc gacacgccaa gctgactgcc
      661 tgaacccttc atctcgcgca cgtttgcagc ccagcgaagg ccttgccggt gtaaacggga
      721 tcgaggatga tgccctcgtt cctcgccatg aacttcacgg cgtcgttgcc ctccttcgag
      781 gggatggcgt aaccctcgcc ccacatgggc gtcagctcca cgtcctcgtt cttgatctcc
      841 aggattaccg gcgcacacgc atcaaacacc ccccgccgcg cgataggcgc agatcggcac
      901 gcttgccggt cgcccgtgac cagcgcccca tcgccgggtc gaacagttca agccgcgagg
      961 gaccgcgaat tccaacgacc gcctcggcct ggtagtccag gttgactacc attacatgcg
     1021 tcggcgtggg cgcacgcttt cctttgcctg ccggaccgaa gtatcccagc agcacgcctt
     1081 tgacgggctg gagattggtg tagctcatgg cgggcaccgg aggatcgagt tggaagcggc
     1141 cgttcgcggg caccggttcg gttccctgcg gcaacatgcc agcgtgataa atacctagcg
     1201 agcacagcgg ctgcagttgc tgcgcgatgg agacgaattc ccggttcagc gtcttcaacg
     1261 tgtagtagag gggagtgacg gtaccgtcgg catgggcgac ggctcctgta tgcccggcgc
     1321 agcaataaac gtaatacgag atcccctgcg ctccataggc gagcgtcgtg taatcgagaa
     1381 aacgcacttc atcacccgtg ggcacccgca tggaaggcga ccatgtggcg gcctgaacga
     1441 tgttgagaaa cggcaggccc gcgtcctgcg ccgcgcgccg gatcatcgcc aggttgagga
     1501 aatatccgtc gttgtcaccc ttcaccgaga attggtagtg gtcgtaactg aggaggccgg
     1561 gcttcacgac gtccacgaat tgccgcagat gctcgcggta ggcggtgacg gtgtcgcctt
     1621 tggtgccaag ctgatcgttg ctcgcatagg tggggaacag gttgatgtag gccagatggg
     1681 ccggatcacg ttcgcgaaga taggcaacga gcttgcccag cgccgaaaag ttggtcgcgc
     1741 ttggctcatc ggtaatgaag taatcgtaaa gcgccgggtg atggcgaacg cgggcaatca
     1801 aggcatccag cttctcgcgt tgcgcggaat cctccagcgt tgtgggcgag agcaaaccgt
     1861 ccgtgagttg gccacgcaag ccgtgccgtt gcaccacatc cagttccttc tcagtgcacc
     1921 agaccaggtt gaacccgccc tcggccatct gggccgcggt cgcgtcggtc atgggcggcc
     1981 cggcccagta ggtggcgatc ggcgtgccca ccttccaggg ggcggccgca ggagcatcgg
     2041 cgccagggca cgagggatag gctgcaaaca gcactccgac cagagcgcca gttaaatgaa
     2101 gagagttcat ggcagttgta atgatcgagt tcgattatat tcgtcgtgcg ggctattgtc
     2161 cattgaacaa tatcccgagc caattcgtca tactcgggac agactaagaa tgtgaacaag
     2221 ccgtactgat gcacgcgcaa cgaaaagccg aactccgctc accgaagcag gaccgcccgg
     2281 gtttcacgct catcgaactg ctggtcgtga ttgcgatcat cgcaactctg gcggcgctgc
     2341 tgctcccggc gctgtccaag gccaagcaga aggcgcaggc cacgcggtgt gtttctcaat
     2401 tgcgtcaatc ttgcgtggcg atgcagcttt atctgccgga ttacagtgac ctcctgtttt
     2461 ggggcgaccc acgctctcca acaatttcca ccgaggggat ggactggttt gtatgggcgg
     2521 ggcgcaccaa taataacttt tataccgggc agcagaacct gttcaatcgt attgatcggc
     2581 ccttgaatca ctatgggctg aattacgcgg tggttacctg cccgctggac cagggccgaa
     2641 ctgaaagcca gccccataag aacatggaat gggtgggtaa ttcgtatctt ttcaattgtt
     2701 tcggactacc gccgggcacg acgggagggc ttgacgggca gcgggcaacc gcgatcaccc
     2761 aaccggcccg gacggtgctc tttgccgact gcatactggc gctgccaacc gagccctacg
     2821 gctggcacgc gatcaaaccc gccgggaacg tggcagccat ggacggccac atcgagcccc
     2881 acacagcaat caacgtaacc aatatgtttt ggtgagtgcc gtcgggcagc ccgcaccgcc
     2941 cgagcggcta cagtttcaac gtgctgccaa cggctccggc cgaggagact cgtcaaccaa
     3001 cttgagcgcc tcagtgacga ggatatcgcc ggtgcgctcg cccaggctgg tccgcaagat
     3061 ataggcatac cgtttgaagc tgtcgaaatc ttcccgggtc aagatgtagt caaacgcatc
     3121 gttggcaagg ccgaagagga ggttgaaaac gggtcccgga attcggaata cattcacccc
     3181 tccacctctc aattattgtt ttcataggcc tgcgccaagc aaccctccaa ccactccagt
     3241 tcggctgtcc ttcgcgcctc acccggccca gagcccgcgt cattctggat ttgtctgccg
     3301 cgtgcgagtg aggtgagggc agaggtgtcc ggcacgcgca ggaaacacaa cctcggaatc
     3361 ctgcccaggt ccaccgagac gccacgctgg ctgcctgaac ccttcatctc gcgtgcgttt
     3421 gtagccgggc ttgttggatc aagaagaaca tgcaaggaca acgcgcgacg gtgaaacata
     3481 tgcgaccggc acgggagtcc aagacaaagg gcaccttgat cgtggagcaa catcgcccca
     3541 tgatgaacaa gctcagcgac gcagagcgcc agcagttgat gcgggcagga atggagttga
     3601 tctatggcgt cccagccgca tcccagccca ctcgtcgccg tagatagcaa cgtgccgctc
     3661 gacttggccg acggcaacga ggcggtcctt gatgccctta atactatccg ggggcgtttg
     3721 tccaacgcgc gctttgtcgt taccccttcg gtatttcaag aactggtcca tgtggcgttg
     3781 gaggattctg tcgctgcccg gcgggcattg gggcaaagcg ctttgcgcca actcaaggag
     3841 tggcagtttg accttctgga aatcgtgccc
//
LOCUS       DATCGS010000085         3170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_304359, whole genome shotgun sequence.
ACCESSION   DATCGS010000085 DATCGS010000000
VERSION     DATCGS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304359"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VIL39_01700"
     CDS             <1..1068
                     /locus_tag="VIL39_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY5480173.1"
                     /translation="EELAEKSTFIEVAHLIIMGKLPNSPELRRFSDLLTDNELLNEAM
                     RHHFEGFPVQAHPMAILSAMINATSCFHPELLTEQMRESFLVQAARLLSKVRTIAAYA
                     YCKTRGLPANYPKPDLKYTANFLHMMFSLPFKDYDLKHETVRALDLIFLLHADHEQNC
                     STATVRMVASSRANLFASAAAGVCALWGHLHGGANQAVLEMLEEIHKSGDDGSKFIAA
                     AKDKNSGKRLMGFGHRVYKNYDPRAKIIKRACDDLLDKLHISDPLLDIAKRLEEAALH
                     DSYFIERKLYPNVDFYSGIIMRAIGIPVEMFTVIFAIGRMPGWIANYKEVMEAPTRIY
                     RPRQIYTGPTLNHYAPIKERN"
     gene            1217..2425
                     /gene="sucC"
                     /locus_tag="VIL39_01705"
     CDS             1217..2425
                     /gene="sucC"
                     /locus_tag="VIL39_01705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5480174.1"
                     /translation="MNIHEYQARQLLGQYGVALPEGDVCDTPEAAKSIAAKLLAEGAN
                     LIAVKSQIHSGGRGRGTFKSGFQGGVKLCKTAEDVGEKAKAMLGNVLVTRQTGPAGRL
                     VSKLLVVAAPNIQKELYLAVLLDRASSRPVVMASTEGGMDIEEVAARTPEKILKESID
                     PAVGMMPYQARKVAAALGLKGELIGLAAKLVLSVYKTWWECDASLVEINPLCVVRTPE
                     GKDAMLAVDAKLSLDDNALYRHPNLQAMRDLAEEAPLEVEASRFNLNYIKLDGNIACL
                     VNGAGLAMATMDIIQHFGGRPANFLDVGGGASQEQVAAAFKIILRDPNVKGILVNIFG
                     GIMDCDVIATGIVAAVKETGLQLPLVVRLEGNNVEAGKRTLADSGLTIIRGGTMADAA
                     QKVVKAVATC"
     gene            2435..2653
                     /locus_tag="VIL39_01710"
     CDS             2435..2653
                     /locus_tag="VIL39_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480175.1"
                     /translation="MLDEALIEQLNRGYVCPPDAGPAWRAAYEAGIDMSLIEHTLELT
                     PAERIQEHQQVLDFLLSIRGGGLANDTE"
     gene            2640..3149
                     /locus_tag="VIL39_01715"
     CDS             2640..3149
                     /locus_tag="VIL39_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480176.1"
                     /translation="MIQNNEALLARLRQNGVQFVVIGGVCSVYYGVPIATFDLDICCQ
                     LGEANLRRIEAAVRDLHPIHRLAANKLPLELTSELCLRLKNLHLQTDLGILDCLGEVA
                     GVGGYEEVYRQSVTGELLSGKSQFLTMDALIAAKQAVGRPRDLETLKHLYAIKEKLAH
                     PTPPPKPKD"
BASE COUNT          654 a          940 c         1009 g          567 t
ORIGIN      
        1 gaggaactgg cggagaaatc cacgttcatc gaggtggccc acctgattat catgggcaag
       61 ctgccgaaca gccctgagct gcggcggttc tcggacctgc tgacggacaa tgagctgttg
      121 aacgaagcga tgcggcacca ctttgaaggc ttcccggttc aagcgcatcc gatggccatt
      181 ctctcggcga tgatcaacgc gacgagctgc tttcatcccg agctgctgac ggagcagatg
      241 cgggagagct tcctggtcca ggccgcgcgc ctgctgtcga aggtgcggac gattgcggcg
      301 tatgcgtatt gcaagacgcg cgggctgccg gccaattacc cgaagcccga tctgaagtac
      361 acggcgaact tcctgcacat gatgttctcg ctgccgttca aggactatga tctcaagcac
      421 gagacggtcc gagcgctgga cttgatcttc ctgctgcacg cggaccacga gcaaaactgc
      481 tcgacggcga cggtgcgcat ggtggcctcg agccgggcga atctgttcgc cagcgcggcg
      541 gccggggtgt gcgcgctgtg ggggcatttg cacggcggcg ccaaccaggc ggtgctggaa
      601 atgctcgaag agatccacaa gtcgggcgac gacggctcga agttcatcgc cgcggccaag
      661 gacaagaaca gcggcaagcg cctcatgggc ttcggccacc gggtctataa gaattatgat
      721 ccccgcgcca agatcatcaa gcgggcctgc gatgacctgc tggacaagct gcatatcagc
      781 gacccgctgc tggacattgc caagcgcctg gaggaggcgg cgctgcacga ttcgtatttc
      841 atcgagcgca agctgtatcc caatgtggac ttctacagcg gcatcatcat gcgcgcgatc
      901 ggtattccgg tcgagatgtt cacggtgata tttgccatcg gccgcatgcc gggctggatc
      961 gccaactaca aggaggtcat ggaagcccca acgcgcatct atcggccgcg gcagatttat
     1021 accgggccca cgctgaatca ttatgcgcct atcaaggagc ggaattgacg gggcgcgggg
     1081 cgttacggcg ccgaatgggc gcaatgagcc gagcgagtta agcaggttga atgggctgcc
     1141 taggttgcct aaggcgcagg agccgggggt gttgccgcca agggtgtgat cgcgccgtgc
     1201 agccataact tccgacatga acattcacga ataccaggcc aggcaattgt tgggccaata
     1261 cggggtcgcc ctgcccgagg gggatgtttg cgacacgccg gaagcggcca agagcatcgc
     1321 cgcgaaactc ctggccgagg gggccaatct cattgccgtc aagtcgcaga ttcattccgg
     1381 cggccggggt agaggcacct tcaagagcgg tttccagggc ggcgtcaagc tgtgcaaaac
     1441 tgccgaggat gtgggggaga aagccaaagc catgctcggc aatgtgctcg tcaccaggca
     1501 aaccggaccc gcgggccggc tcgtcagcaa gctgctggtg gtcgccgcgc cgaacatcca
     1561 gaaggagctt tacctggcgg tgctgctcga ccgcgcgtcg tcgcgcccgg tggtcatggc
     1621 cagcaccgaa ggcggcatgg acatcgagga ggtggcggcc cggacgccgg agaagattct
     1681 caaggaatcc atcgaccccg ccgtcggcat gatgccttac caggcccgca aagtcgccgc
     1741 cgcgctgggg ctcaagggcg agttgatcgg cctggcggcc aaactggtgc tgagcgttta
     1801 caagacgtgg tgggagtgtg acgcgtcact ggtggagatt aatccccttt gcgtcgtccg
     1861 caccccggaa ggcaaagacg ccatgctcgc ggtggacgcg aagctttcgc tcgatgacaa
     1921 cgcgctttac cggcatccga acctccaggc gatgcgcgac ctggccgagg aagcgccgct
     1981 ggaagtcgag gcgagcaggt tcaacctgaa ctacatcaag ctcgatggca acatcgcctg
     2041 cctggtcaat ggcgccggcc tggcgatggc cacgatggac atcatccagc actttggcgg
     2101 acggccggcc aacttcctgg acgtcggtgg cggcgccagc caggagcagg tcgcggcggc
     2161 cttcaagatc atcctgcggg acccgaacgt gaagggcatc ctggtcaaca tcttcggcgg
     2221 catcatggac tgcgacgtca tcgccaccgg catcgtggcc gcagtgaaag aaaccggtct
     2281 ccagctcccg ctggtggtgc ggctcgaagg taataacgtc gaagccggca agcggaccct
     2341 ggccgattcc gggctcacca tcatccgcgg cggcacgatg gccgacgccg cgcaaaaggt
     2401 ggtcaaagcg gttgccacct gctaaggtgt atctgtgctc gacgaagcgc tcatcgaaca
     2461 actcaaccgt ggctacgtct gtccgcccga cgccggcccg gcgtggcgcg cggcgtacga
     2521 ggccgggatc gacatgtcct tgattgaaca cactctcgaa ctcactccgg ctgagcggat
     2581 tcaggagcac caacaggtac tcgactttct gttaagcatt cgcgggggcg gtctggcaaa
     2641 tgatacagaa taatgaagcg ttgctggccc ggctccggca gaacggcgtt caattcgtag
     2701 taattggcgg cgtttgcagc gtttactatg gcgtgccgat tgccacgttc gacctcgaca
     2761 tctgctgtca acttggcgaa gcaaatttgc ggcgcattga ggctgcggtg agggatttgc
     2821 acccgatcca ccggctggca gcaaacaaat tgccgctgga actgacctcc gagctatgcc
     2881 ttcgcctcaa gaatttgcat ctgcagaccg acctgggcat cctggactgc ttgggtgaag
     2941 tcgcgggagt cggtggctac gaggaagtgt atcggcaaag cgtgaccgga gagctgttgt
     3001 ccggtaaatc ccagttcctg actatggatg ccctgatcgc cgccaaacaa gctgtggggc
     3061 ggccccgtga tcttgaaacc ttaaagcatt tgtacgcaat caaggagaag ctcgcacacc
     3121 cgacgccacc gccaaaaccc aaagactgac gcctgcatcc ccccccaata
//
LOCUS       DATCGS010000086         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_156165, whole genome shotgun sequence.
ACCESSION   DATCGS010000086 DATCGS010000000
VERSION     DATCGS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156165"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /gene="waaF"
                     /locus_tag="VIL39_01720"
     CDS             complement(<1..1293)
                     /gene="waaF"
                     /locus_tag="VIL39_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY5480177.1"
                     /translation="MTSSRPAATPHPRRILVRGVNWLGDAVMTTPALQRLRERFPNAQ
                     ITLLTNEKLAALWQQHPSIDAVMTFSSKQGPFAVARRIRCWHFAQGLVDIQCLLKKDY
                     PQTFAGMAERGCDELAQKTVELYARFGAWSPQLFDIALVLPSSPRSALEMWLARIPHR
                     IGYARPWRNWLLTHPIPSRPDHEQMRKRSVSEINRLVRSPATEAHGPPRITHQIHDYL
                     HLAATLGASPEPLPPKLEISAAEIRQAEDAFLSDARNKVPGSAPGKPLVLLGLNPGAE
                     YGPAKRWPAERFAVVVREISHRLGNCVWLAFGVKGDWELCDEVARLAGGGVLNLAGKT
                     SLRQLMALLKLCRVVLSNDTGPMHVAAALGIPVVVPFGSTSPELTGPGSPGDPRHHLL
                     RSSAPCSPCFRRACPIDFRCMTGISTERVVAAVQAALTP"
     gene            complement(1290..2309)
                     /locus_tag="VIL39_01725"
     CDS             complement(1290..2309)
                     /locus_tag="VIL39_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480178.1"
                     /translation="METLLYCVVRALVAFLQALPLTWVARLGRLGGGLAYWVDARHRR
                     VALHNLTMCFGHEKSPAELRALAHENFRRLGENYACGIKTAAMSFEVLRSRVEFVGDA
                     RIVSPPTDQKPQGVVAAIGHFGNFELYARFGQFAPAYKCATTYRGLRQPSLNRLLQSL
                     RERSGCLYFERRFEAPALKAFMNQPGGILGLLSDQSSGTLRVPFLGHDCSTTAAPAIF
                     ALRYHCALITGICYRVGLARWRIEAGGEIPTRENGQPRSTAAIMGDVNRAFEAAVRRD
                     PANWFWVHKRWKPARSGAGVPPARTGVLPAVDERGTKGPEGRRDACPTTSQMLPGSQN
                     SEAGQ"
     gene            complement(2312..3484)
                     /gene="lpxK"
                     /locus_tag="VIL39_01730"
     CDS             complement(2312..3484)
                     /gene="lpxK"
                     /locus_tag="VIL39_01730"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY5480179.1"
                     /translation="MREQLRAWTETLETFFQEVIVEERRGTRETLVRALLYGCSKVFE
                     AAIKIRRFLYNVRILRDSTLGVQVIAVGNLTVGGTGKTPVVEKFARELRDQGRNVAIL
                     SRGYRSKPIPLHQRLLNKLLLRTDTTPPRVVSDGKSLLLDSDMAGDEPYMLASNLKDV
                     VVLVDKDRVKSGRYAIEKFGCDTLLLDDGFQYWKLRGRRQDIVLIDQQQPFGTERLLP
                     RGTLREPPSHLARASTIFITKSDGNTAELRRRLAQFNSTAGIIECIHHPLYLEDVFTG
                     QRLGLESLKGRKVASLSGIAQPESFEQSLVRLGAELVYSKRFADHHRFTQQEILNAIN
                     RSKKRQADTIVTTQKDAVRFPKIDRRDLPFYFMRVEIKILRGADDFQDCVRKICFR"
     gene            complement(3655..4356)
                     /locus_tag="VIL39_01735"
     CDS             complement(3655..4356)
                     /locus_tag="VIL39_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480180.1"
                     /translation="MSAKKQIIKVLVADDHPVVRKGLQTCLAKHGTLRIVGEAADGDE
                     ALRKARELSPDVVLMDISMPGMNGLAVTEILRKEAPDMKVLILSVHSNKEYIFRIIHA
                     GAHGYVSKEAPPEELIRAIESVYAGEPHFSEDIARAALSEFVNTGGKKEPFAQLTTRE
                     REVLVLIAEGKGNKEIADQLGIGVRTIETHRERIMRRLNIHSVAGLTKYAIANGLIVL
                     ESGSGPNSSLGNQKY"
BASE COUNT          849 a         1372 c         1547 g          853 t
ORIGIN      
        1 gggcgtgaga gccgcttgga cggccgccac aacacgctcc gtgctgatgc cggtcatgca
       61 acggaagtcg atgggacagg cacggcgaaa gcagggcgag cagggcgcgg aagatcgtag
      121 taagtggtgg cgggggtcac ccggagaacc cggcccggtc agctccggcg acgtgctgcc
      181 aaagggcaca acgactggta tccccagggc ggcggcgacg tgcatgggac cggtgtcgtt
      241 gctgagaacc acgcggcaga gcttgagcaa cgccatgagt tgccgcagcg aagtcttccc
      301 ggccaggttg agaacgcccc cgcctgcaag ccgggcaacc tcgtcgcaga gttcccaatc
      361 acccttgacc ccaaacgcca gccagacgca atttccaagc cggtgggaga tttcacgcac
      421 gacgaccgca aagcgctctg cgggccagcg cttggcgggg ccgtattcgg cgccgggatt
      481 cagcccaagc aggaccaagg gtttgccggg tgcggagccc ggcaccttgt tgcgcgcgtc
      541 ggataagaag gcatcctccg cctggcgtat ctccgcggcg gagatctcga gcttcggtgg
      601 gagcggttcg ggactcgcgc cgagagtggc tgccaggtgg aggtaatcgt ggatttggtg
      661 ggtgatgcgt ggaggaccgt gcgcttcggt ggccggggag cggaccaagc gattaatctc
      721 actgacggac cgtttgcgca tttgctcatg gtccggccgg gaggggatcg ggtgagtaag
      781 gagccagttg cgccagggac gggcgtagcc gatgcggtgc ggaattcgcg cgagccacat
      841 ctccagcgct gaacgggggg aactgggcag gaccagggcg atgtcaaaca actgagggct
      901 ccaagcgccg aaccgtgcat atagctccac cgtcttttgc gccagctcat cgcagcctct
      961 ttcagccatg ccggcaaagg tctgaggata gtccttcttc agtaaacact ggatgtcaac
     1021 cagcccttga gcgaagtgcc agcagcggat gcggcgagcg acagcaaaag gaccctgttt
     1081 ggagctgaat gtcatcacag catcaatgct cggatgctgc tgccaaaggg ctgcgagctt
     1141 ctcattcgtg aggagcgtta tttgcgcatt gggaaatcgc tcgcgcaggc gctgcaaggc
     1201 gggcgtcgtc atgaccgcgt cacccagcca attgacgccg cgcacaagga tgcggcgggg
     1261 gtggggggtg gcggcgggac gagagcttgt cattgcccag cttcggagtt ctggcttcca
     1321 gggagcatct gcgaggtagt ggggcaggcg tcccgcctgc cctcagggcc tttggtccca
     1381 cgctcgtcca ctgcgggcaa gacgcccgtg cgggcaggcg ggacgcctgc cccactcctg
     1441 gcgggtttcc atcgtttgtg cacccagaac cagttggcgg ggtcgcggcg aacggcggcc
     1501 tcgaacgctc ggttcacgtc gcccatgatg gcggcggtgg aacgaggctg accattctcg
     1561 cgggttggaa tttcgccgcc tgcctcgatg cgccagcggg ccaagccgac ccgatagcag
     1621 atgccggtga tcagcgcgca gtggtagcgc agagcgaaga ttgccggcgc ggccgtcgtc
     1681 gagcagtcgt gcccaaggaa gggcacgcgc agtgtgccgg acgactgatc agacaggagc
     1741 cccagaatgc cgccaggctg gttcataaag gcctttaacg ccggggcctc gaatcggcgc
     1801 tcaaagtaga ggcagcccga acgctcgcgc agcgattgca gcaggcggtt gagtgacggc
     1861 tggcgcaaac cacggtaggt cgtggcgcat ttgtaggcgg gcgcaaactg gccgaagcgg
     1921 gcatacagct cgaagtttcc gaagtggccg atcgcggcaa ccacgccctg cggcttctga
     1981 tccgtggggg gtgaaacgat ccgggcgtcg ccgacgaact ccacccggga acgcagcacc
     2041 tcgaagctca tggcggcggt cttgatgccg caggcatagt tctcgccgag gcgtcggaaa
     2101 ttctcgtgcg cgagggcgcg gagttcggcg ggcgatttct cgtggccgaa gcacatcgtc
     2161 agattgtgca gggccacgcg ccggtgccgc gcatccaccc agtaggccag gccgccgcca
     2221 agccgcccca gccgggccac ccaggtcagc ggcagcgcct ggagaaaggc aaccagcgcg
     2281 cgcaccacgc agtaaaggag tgtttccatc gttagcggaa gcagatcttg cgcacgcagt
     2341 cctggaagtc atccgcgccg cggaggatct tgatctcgac gcgcatgaaa tagaatggca
     2401 agtcgcgccg gtcaatcttg gggaagcgca ccgcgtcctt ctgcgtggtg acaatcgtat
     2461 cggcctggcg cttcttgctg cggttgatgg cgttcaggat ttcctgctgc gtaaagcgat
     2521 ggtggtccgc aaatcgcttg gaatagacca gctccgcgcc gagccggacg aggctttgct
     2581 cgaagctctc gggctgcgcg atgccgctca gggacgctac cttgcgtccc ttcaacgact
     2641 cgagcccgag gcgctggccg gtgaacacat cctccagata cagcgggtga tggatgcatt
     2701 cgatgatgcc ggctgtcgag ttgaactggg cgaggcggcg gcgcagctcg gccgtattgc
     2761 cgtcgctctt ggtgatgaag atcgtgctgg cgcgggcaag gtgggacggc ggctcgcgca
     2821 aggtgccgcg cgggagcaac cgctcggtgc cgaagggctg ctgctggtca atcaacacga
     2881 tgtcctgccg ccggccgcgg agcttccagt actggaaacc gtcgtcgagc agcagggtgt
     2941 cgcagccgaa cttctcaatc gcatagcggc cgctcttgac gcggtccttg tccaccagca
     3001 ccaccacatc cttgagattg gaggcgagca tgtaaggttc gtcgccggcc atgtcggaat
     3061 cgagcagcag cgatttgccg tccgacacga cgcgcggcgg ggtggtgtcg gtgcggagaa
     3121 gaagcttgtt cagcagccgt tggtgaagcg ggatcggctt ggaccggtag ccgcgcgaga
     3181 ggatggcgac gttgcgtccc tggtcgcgca gctcgcgggc gaacttctcg accaccggcg
     3241 tcttcccggt gccgccgacc gtaaggttgc cgaccgcgat gacctgcacc ccgagcgtcg
     3301 agtcccgcag gatgcgaacg ttgtaaagga agcggcggat cttgatggcc gcttcgaaga
     3361 ctttggagca gccgtagagc agggcacgca cgagggtctc gcgcgtccca cgccgctcct
     3421 cgacgatcac ttcctgaaag aaggtctcaa gggtctctgt ccacgcacga agctgctcac
     3481 gcatctgcgg tcagtgtgcc aatcccttgg aggcagggca agcggaaggc tatgggtgat
     3541 ccctggggtg gggagacttg gaagcctggg atcctcgccg gaacggcgaa cggttaatcc
     3601 tgccacttcc agcggaaccc cgaatcttcg ccggcagggt ctttgacgcc cggctcagta
     3661 tttctgattg ccgagactgc tgttcggccc cgacccgctt tccagcacga ttagcccgtt
     3721 ggcaatggcg tatttggtga gtccggccac cgagtggata ttgagccggc gcatgattcg
     3781 ttcccggtgc gtttcgatgg tgcggacgcc gatgccaagc tggtcggcga tttccttgtt
     3841 gcccttgcct tccgcaatga gaacaagaac ctcccgttcg cgggtggtga gctgggcaaa
     3901 aggttccttc ttgccgccgg tgtttacgaa ttcgctgagc gcggcgcggg cgatatcctc
     3961 gctaaaatgg ggttcgccgg catacaccga ttcaatggcg cggatcaatt cctccggggg
     4021 cgcttctttg gagacatacc cgtgcgcacc ggcgtggatg atccggaaga tgtactcttt
     4081 gttactgtgc accgagagga tcagcacctt catgtccggc gcttccttgc ggaggatttc
     4141 tgtgacggcc aagccattca tgcccggcat gctgatgtcc atcaggacga cgtccggtga
     4201 cagttcacgc gctttgcgca gggcttcatc cccgtccgcc gcctctccca caattctcag
     4261 tgttccatgc ttggcgaggc aggtttgcag ccccttccgg acgaccggat gatcgtccgc
     4321 taccaatacc ttaattatct gttttttcgc gctcaccgtt tgccttgtcg gctgcgaggc
     4381 ggtttcaggc cccgctgcag tccagtgcgg cagaattaaa tccaatcccg gttcgggtgt
     4441 aaagagagat tgtcaggacc tggaagccta aatcttcccc gaacggtggt gaagactccc
     4501 ccgataagcc gcagttgaat ccgccagcga gttcagagcg agcttgccac tttcgagggg
     4561 cactggtatg caagccgccc atgaatagag gcataagcgg cttttagacc ggcgtcatgg
     4621 a
//
LOCUS       DATCGS010000087         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_552493, whole genome shotgun sequence.
ACCESSION   DATCGS010000087 DATCGS010000000
VERSION     DATCGS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552493"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..3288)
                     /locus_tag="VIL39_01740"
     CDS             complement(223..3288)
                     /locus_tag="VIL39_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5480181.1"
                     /translation="MRRRLFRYDKLVVLWLCLGSAGGGFAVETTVLQDVVLRNWDLDD
                     GLPSARINAVARTPDGYLWLATQKGVVRFDGTHFAVFDTSNTPKMADDRVSCLLLDRR
                     GDLWAGTCGGTLLKREGQVFRAQDLGTAALSPKEPGTANGKVNALAEDGQGALWLALE
                     GMGLLRFKSGQAQAFGTRNGLPSMDVRKVLCDGDGRLWVVAGGQLGIFEGVRWREPSG
                     LSPASQTVRTISPARDGGLWVATGTVDPLASRDLRLYKLRGGQWSAQLEPYPWPPDSQ
                     QFQRLALLEDQSGRIWCAAAGGVFVHTPGGSWQRLLSVAPWVQVEVLCLAEDENDLLW
                     MGTRTTGLLQVQKRLVKTLPLPAFASQHAVLTACASKEGSVWCGTDGAGIFRWQAEQM
                     TSFGTEQGLISLHVAALLEDHHTNLWAGTHAGLFRRVGERFEPVPGPPALREPILALL
                     EDRQGNLWTGGCAGLVRLDEQGARFFGTERDLFGGAVRALEEDHDGTIWVGPNAGLYW
                     LKSKLFDYYPVPQEPHLQGIFALHCDTSGSLWIGTELAGLLRLRAGHFDQWLWPRDGL
                     PSNHITAILEDNEGNLWMSSENGIFACSKTELDNYQRGVTPPLRPRRLTPAEGLVHKV
                     CSGVGQPSACKSADGRLWFPDGPALAVFDPATIARAVRVWPPLVEGISVDGVPATLVP
                     GGFRVRSGARRIEFQYTSPNVLIPERLRFHFRLVGLDKEWVDAGPRREASYSQLRPGS
                     YEFKVRASGPEGAWQEGGGLQLEILPRWWERTFVQLAAGLALLAVVAGTVWALERGRS
                     RRRLERLELQRTLDAERQRIARDIHDDLGSGLTEIILLSDSLDEAIQPTPADQKMVGE
                     ISARARSLTRAMDEVVWAINPRNDTLEGFLTYLGKFTQDYLSKAEVRCRWNVPVEVPE
                     LPLSADMRHSLYLACKEALHNLIKHAGASEVSVQLELADGGFTLSIEDDGHGFAANCP
                     PARGNGLPNMRQRLKELGGLCRVESTPGRGTRVVFSLPAAIQRNLSAVK"
BASE COUNT          708 a         1180 c         1115 g          616 t
ORIGIN      
        1 cccggaggag tattgaccga cacaggcaca gccgccaaag cgcgcgacag cgttcacgag
       61 cttggtccgc acgccgtcat catcatccac tatggcgacc ttaataggca ttggcttctt
      121 ttgtcggcag gaagttaagg gcgcagagag tgttttcata ctggccaaaa ctggctatgc
      181 gacctgggat tgcgagtcgg acggagaatc gggcggcgtt gactatttca cggcggaaag
      241 gtttcgctga atggcggccg ggagcgaaaa caccacccgc gtgccccggc ccggggtgct
      301 ctccacccgg cagaggccgc ccagctcttt gagccgctgg cgcatgttgg ggagtccatt
      361 cccgcgagcc ggcgggcagt tagcagcaaa gccgtggccg tcgtcttcga tggacagggt
      421 gaagcctccg tcggccagct cgagctggac cgagacttct gaggcgccgg cgtgcttgat
      481 caggttgtga agggcttcct tgcacgcgag gtaaaggctg tgccgcatgt cggcggagag
      541 aggcagttcg ggcacctcga ccgggacgtt ccagcgacac cggacctcgg ccttgctgag
      601 atagtcctgg gtgaatttgc cgagataggt caggaaaccc tcgagcgtat cattgcgcgg
      661 gttgatggcc cagacgactt cgtccatggc ccgggtcagc gagcgggcgc gagcggaaat
      721 ctcacccacc atcttctggt cggcgggagt cggctggatg gcctcgtcca agctgtcgct
      781 tagcaggata atctcagtca ggccggaccc taaatcgtca tgaatatcgc gagcaatgcg
      841 ttgacgttcg gcgtccaagg ttcgttgcaa ctccaaacgt tccaaacgtc ggcgcgagcg
      901 gccccgttcc aaggcccaga ccgtgccagc cacgacggcc aataaggcta atccggcagc
      961 cagttgaacg aaggttcttt cccaccagcg gggcaggatt tccagttgca gtccgccccc
     1021 ctcctgccag gccccctcgg gcccgctggc tcgcactttg aattcatagc tcccgggccg
     1081 caattgagag taggaagctt cgcgtcgggg accggcatcc acccactcct tgtccaaccc
     1141 taccagtcgg aaatgaaacc gcagccgctc cgggataagc acgttgggcg aagtgtattg
     1201 aaactcgatg cgtcgcgcgc cggaccgcac gcggaacccg ccgggaacca aggtggcggg
     1261 cacgccatcc actgagatcc cttcgaccag gggtggccag acgcgaaccg cgcgcgcaat
     1321 cgttgccggg tcgaacaccg cgagggcggg cccgtcggga aaccagagcc gcccgtccgc
     1381 cgacttgcac gccgagggtt ggccgactcc cgagcagacc ttgtggacca agccttccgc
     1441 cggcgtcagc cgtctgggcc gcaggggcgg agtgacccct cgttggtagt tgtccaactc
     1501 cgtcttggaa caggcgaaaa tgccgttttc cgaactcatc cagagattcc cctcattgtc
     1561 ttcgagaatg gccgtaatgt ggttgctcgg cagaccgtca cgcggccaaa gccattgatc
     1621 gaagtgaccg gcgcggagcc gcagcagtcc ggccagctcg gtgccaatcc aaagcgagcc
     1681 cgaggtgtcg cagtgaaggg caaaaatacc ttgcaaatgg ggttcctgcg gcaccggata
     1741 ataatcgaac agcttgctct ttagccagta caggccggcg ttggggccca cccaaatcgt
     1801 gccgtcatgg tcttcctcca gcgcccgaac ggctcccccg aacaggtccc tttccgtgcc
     1861 aaagaatctt gcgccctgct cgtcaagccg aaccaggccc gcgcaccctc ccgtccacaa
     1921 gttcccctgg cggtcctcga gcagcgccag gatcggctcg cgcaaggccg gcgggccggg
     1981 caccggttcg aaccgctcgc ccacccggcg aaataggccg gcatgggttc cggcccacag
     2041 gttggtgtga tggtcctcga ggagcgcggc aacatgcaga ctgatcaatc cctgttcggt
     2101 cccgaaggaa gtcatctgct cggcctgcca gcggaagatg cccgcgccat cggttccgca
     2161 ccagacactg ccctccttcg aggcgcaggc ggtgagcacg gcatgctggc tggcaaaggc
     2221 cggcaggggc aaggtcttca ccagacgctt ctggacttgc agcaaaccgg tcgtgcgtgt
     2281 gcccatccag agcaggtcgt tttcatcctc ggccagacac aggacttcga cctggaccca
     2341 gggggcaacc gacaacagcc gctgccagga gccgcccggg gtatgaacga agacgccacc
     2401 cgcggcggca caccaaatgc gcccgctctg atcctcaagc aacgcgagcc tttggaattg
     2461 ttgcgagtcc ggcggccaag gatagggctc caattgggcg ctccattgtc cacctctgag
     2521 cttatacaag cgcaagtccc ggctggcgag cgggtccacc gtgccggtgg cgacccacag
     2581 tccgccatcg cgagccgggc tgatggtccg cacggtctgg gaggcgggtg acaaaccgct
     2641 cggttctcgc caacggacgc cttcgaagat gccaagctgc ccgcccgcta ccacccacaa
     2701 tcggccgtcg ccgtcgcaca agacttttct gacatccatc gagggcaggc cgttcctggt
     2761 gccaaaagcc tgggcctgtc cgctcttgaa ccggagcaac cccatgcctt cgagggccag
     2821 ccataaagcc ccctggccat cctcagccaa agcgttgacc ttcccgttgg ccgtgccggg
     2881 ctccttgggg gacagcgcgg cggtccccaa atcctgcgcg cgaaagacct ggccctcccg
     2941 cttcagcaag gttccgccgc acgtgccggc ccacaaatcg cctcgccggt ccaacagcag
     3001 gcacgagacc cgatcgtctg ccatcttcgg cgtgttggag gtatcaaaga cggcgaaatg
     3061 agtgccgtcg aaacgcacca cgcccttttg cgtggcgagc cagagataac cgtcgggcgt
     3121 gcgggcgacc gcgttgatcc gcgcgctagg caggccgtca tcgagatccc agttcctcaa
     3181 gaccacatcc tggaggactg tcgtctcgac ggcgaaaccg ccgcccgctg acccgaggca
     3241 tagccacaga actacaagct tgtcgtatcg gaacagccgg cgtctcaacg ctcgatgaga
     3301 cgaggggcac attgcaccta cgacgctagc cagttttcgg ggccagagca agcgcggcaa
     3361 ccacgggaaa ctcgtggagt tcgccctacg cttacgaggc ttgtgacgac gtggacccga
     3421 cccaaaagta tgagaacgtg ggcaggactg agacgcatcc ccaagctgtc aggcctgaat
     3481 gccggagtgc gcctaatcgc ccggaaacgg tgaattgccg gggagccatg gtcattgtgg
     3541 cacttgcgga cccccttaag ataccggatt ggttgcctac cagttcccaa gcaacacgcc
     3601 tgcgggtagc ttggggggc
//
LOCUS       DATCGS010000088         4164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_353967, whole genome shotgun sequence.
ACCESSION   DATCGS010000088 DATCGS010000000
VERSION     DATCGS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353967"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1309
                     /locus_tag="VIL39_01745"
     CDS             227..1309
                     /locus_tag="VIL39_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY5480182.1"
                     /translation="MKHALNIPKSGSLDFVSVGALVHRLDPGVIPFRKATQCQIHVSG
                     GEFNVAANLADCFGLQTGIATAMVDYPVGDLIAERVRAMGVKPFYKHFKHDGVHGPNM
                     ATVYSDRGQGVRAPVVFYNRCNEAGALLKPGDFDWKAIFAGGVRWFHSGGIFAALSPT
                     TAEVIIEGMQAAKAAGAVVSFDLNYRAKLWNISGGHDRAVAVLDRIVKNVDVLVGNEE
                     DLQLGLGIPGPETAAKHKLDPSAFIGMIGKVVKKHPQIKIVATTLREVHCTNRHTWGA
                     VAWINGETVMAPACELDVLDRVGGGDGYASGFFYGLLAGEDPAEAVKLGWAHGALLTT
                     FPGDTTMATLDQVRAFAKGGSARIQR"
     gene            complement(1422..2828)
                     /locus_tag="VIL39_01750"
     CDS             complement(1422..2828)
                     /locus_tag="VIL39_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480183.1"
                     /translation="MEELEVHGMAEKQTDILSKPAERASFPPGLHNAYLFSVFNAFSY
                     QIVLSSPMVLYAKTLGASATVLGIVVGMMPLLVIFQIPAANYIPRLGFKQFVYAGWGT
                     RVMFIFAMALVPLTGFFLKPETLLALMLMLLFGFNLSRGISSCAWLPWITALVPATLR
                     GKYLARDAAVQSLASFITFLIAAACLAGDPRSWQFAILFAFSAVMGAVSLTFLKRIPD
                     AEMPEEIHASKGRVPWLEMLRFPPFRKLLLTLIGYSVAYGGMTAFTVAFLKTETDMSE
                     GKILLVNSVSYLGGLGSLWFLGSRLDHLGSKPVLTFCFSAWVAVLAGWIALSGGVFPV
                     RLSIILLLQFMMGLLAALIQMAITRLAMATIPVMGRNHFFAIYSTLGNVTLGLAPIAW
                     GLLIDAVGTRSPIWLGVAWNRYSVFFAAAALFFTITLTLARRLHEPEAASMEDLLREI
                     LIQSPQRFWIRFWPRGSA"
     gene            2998..>4164
                     /locus_tag="VIL39_01755"
     CDS             2998..>4164
                     /locus_tag="VIL39_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480184.1"
                     /translation="MNKDSSSRRSFLKTASVAATAITIIPRHVLGQGQTPPSQKLNIA
                     SIGLGGMGSGDVDSCAKAGQNIVALCDPDRNHLAENAKKYPGAKLYTDFRKMLETQKD
                     IDAVTIATPDHVHAVATAMAMKLGKHVHCQKPLTHSVYEARMIGKIARETKVATQMGN
                     FGQAGEEPRRVAELIWAGAIGTLKEVHAWSNRNPDISPRGVARPKDTPPCPAHLEWDL
                     WVGPAPTRPYHPCYHPFAWRGWWDFGTGVLGDIGCHELSGIFKAMKIGDPVSVEACST
                     NWQRPKEISTETAPLASITHYKFAASATHGPLSIFWYDGGMRPPRPEELEPDRPFAEN
                     DGTMYVGDKGVMLRDRLIPDKKWNEFGRAPQKLARSPGHWQEWLDACRGGKPAGS"
BASE COUNT          867 a         1277 c         1321 g          699 t
ORIGIN      
        1 ctcacttaca cctgcttccc agcatagcgc attcctgtaa taagaccaag accgtgactg
       61 gaacattcca gccccgaaac gcgagccgct tcgaaacggc tctgcgccgc tctgacgagg
      121 actcagatgg gtggcattcc tgcgttgcgc ccgaagcttg ccgcccgtca actttttggc
      181 agacaaacca gcctgcttca gcgtattatc ccataactaa gtcatcatga aacatgcttt
      241 aaatatccct aagagtggat cgctcgactt cgtgtccgta ggcgctctgg tccatcgcct
      301 tgatccgggc gtcatcccct tccgcaaggc cactcaatgc caaatccacg ttagcggcgg
      361 cgagtttaac gttgcggcca acctggcgga ctgcttcggt ctgcagaccg gcatcgccac
      421 tgcgatggtg gattaccccg tcggcgacct gattgccgag cgcgtgcggg ctatgggcgt
      481 caagcccttc tacaagcact tcaagcacga cggcgtgcat ggtccgaaca tggccaccgt
      541 ctatagcgac cgcggccagg gcgtgcgcgc acccgtggtc ttttacaacc gctgcaacga
      601 ggccggcgcc ctgctcaagc cgggcgattt cgattggaag gccatttttg ccggtggcgt
      661 ccgctggttc cacagcggcg gcatcttcgc ggccctctcg ccgaccacgg ccgaagtcat
      721 catcgagggc atgcaagcgg cgaaggccgc tggcgcggtg gtctccttcg atctcaatta
      781 ccgggccaag ctctggaaca tctcaggcgg ccacgaccgc gcggtggcag tgctcgatcg
      841 catcgtgaag aatgtggatg tgctcgtcgg gaacgaggaa gacctccagt tgggactcgg
      901 catcccgggc ccggaaactg ccgcgaagca caagctcgac cccagcgcct tcatcgggat
      961 gatcggcaaa gtggttaaga agcatcccca gatcaagatt gtcgccacca ccctccgcga
     1021 ggtgcattgc accaaccggc acacctgggg cgcggtggcc tggatcaatg gcgagaccgt
     1081 catggcaccc gcctgcgaac tcgacgtgct cgatcgcgtc ggcggcggcg acggctacgc
     1141 gtcgggcttc ttctacggcc tgcttgctgg agaagacccg gcggaggcgg tcaagctggg
     1201 ttgggcgcac ggcgcgctgc tgactacctt ccccggcgac accactatgg cgacgctgga
     1261 ccaggtgaga gcctttgcca aaggcggctc ggcgcggatt caacgctgac ctgcccacca
     1321 ggtgcttcag gaacaggccc tggacgatgt ggattggcgc aacggtgggt tgcgattggc
     1381 cgctcgcaag ggccggcgag acggtgcctt gacagggcgc atcacgcgct tcctcggggc
     1441 cagaaacgga tccagaagcg ctggggggat tggatgagga tttcgcgcag gagatcttcc
     1501 atgctggcgg cttcgggttc gtggagccgc cgcgccaggg taagcgtgat cgtgaagaag
     1561 agggcggcgg cggcgaagaa cacggagtaa cggttccagg cgacgccaag ccagatgggc
     1621 gagcgggttc cgacggcatc aattagcagg ccccaggcga tgggcgcgag accgagggtg
     1681 acgttgccga gggtcgagta gatggcgaag aaatggttgc ggcccatgac gggaatggtt
     1741 gccatggcga ggcgcgtgat ggccatttgg atcagcgcag ccagcaggcc catcatgaac
     1801 tggagcagga ggatgatgga cagccgcacc gggaaaaccc cgccggacag ggcgatccac
     1861 ccggccaaga ccgccaccca ggcggaaaag caaaacgtca gcactggttt actgcccaag
     1921 tgatccagtc gcgagcccag aaaccaaagg ctgcccaagc cgccgagata ggaaacggag
     1981 ttgaccagca ggattttgcc ttcggacatg tcggtctcgg tcttgaggaa ggcgacggtg
     2041 aaagcggtca tgccgccgta ggcgacggag tagccgatga gggtgagcag cagttttcgg
     2101 aagggcggga agcgcagcat ttccagccag ggcacccggc ctttggaagc gtggatttcc
     2161 tccggcatct cggcgtcggg gatgcgtttg aggaaggtga ggctcaccgc gcccatgacg
     2221 gcgctaaagg caaagaggat ggcgaattgc caggaacgcg ggtcgccggc caggcaggcg
     2281 gcggcgatga ggaacgtgat gaagctggcg aggctctgga cggcggcgtc gcgggccagg
     2341 tatttgccgc gcaacgtggc ggggaccagc gccgtgatcc aaggcagcca ggcgcaactg
     2401 gagatgccgc gcgagaggtt gaagccaaac agcagcatca acatcagggc gagcagggtt
     2461 tcgggtttga gaaaaaagcc ggtcagcggc accagcgcca tggcgaagat aaacatcaca
     2521 cgcgtgcccc agcccgcata cacaaactgc ttaaacccga ggcgggggat gtaattcgcc
     2581 gccgggattt ggaagatgac cagcagcggc atcatgccga cgacaatgcc gagcacggtg
     2641 gcgctggcgc cgagggtttt ggcgtagagc accattgggc tgctcaggac gatctggtag
     2701 gagaaggcgt tgaagaccga gaacagatag gcgttgtgca ggccgggagg aaaggaggcc
     2761 ctttccgccg gcttgctcaa aatgtctgtt tgcttctcag ccatgccgtg cacttccaac
     2821 tcttccattc aaccacgaag ggaggcggat gaacacggat aaatagcaag cggtaccgta
     2881 atgcccaact ttccttacga caccgtattt agggccgcac atacttggat tgaccttcgg
     2941 ctccacaagt ctaagcttgc tccagatgaa tcctaactcg caaagaaaga tcacgccatg
     3001 aacaaagact cttcctcccg ccggagcttc ctgaaaacgg cttctgtcgc cgcaacggct
     3061 atcaccatta tccctcgtca cgtgctgggg cagggtcaaa ccccgccgtc gcagaagctc
     3121 aacatcgcga gcatcggtct cggcggcatg ggctccggcg atgttgactc gtgcgcgaag
     3181 gctgggcaga acattgtcgc cctttgcgat cccgatcgta accacctggc cgaaaacgcc
     3241 aagaaatacc cgggtgccaa gctttacacc gatttccgca aaatgcttga gacgcagaag
     3301 gacattgacg cagtgaccat cgccaccccc gaccacgtgc atgccgttgc caccgccatg
     3361 gccatgaagc tcggcaaaca tgtgcactgc cagaagccgc tcacccacag tgtttacgag
     3421 gcgcgcatga tcggtaagat cgcacgtgag accaaggtag ccacccagat gggcaatttc
     3481 gggcaggccg gcgaagagcc ccgccgcgtt gccgagctga tctgggccgg cgccatcggc
     3541 acactcaagg aggtgcatgc gtggtccaac cgcaacccgg acatctcccc gcgcggtgtt
     3601 gcccggccca aggacactcc gccctgcccg gcgcacctcg agtgggacct gtgggttggc
     3661 cccgcgccca cgcggcctta ccacccgtgc taccacccct ttgcctggcg cggctggtgg
     3721 gatttcggca ccggcgtgct cggcgacatc ggttgccatg agctctccgg catcttcaag
     3781 gccatgaaga tcggcgaccc ggtcagcgtc gaggcctgct ccacgaactg gcagcgcccc
     3841 aaggaaatca gcaccgagac cgccccgttg gcttcgatca cccattacaa gtttgccgcc
     3901 agcgccactc acgggccgtt gagcatcttc tggtacgacg gcggcatgcg cccgcccagg
     3961 cccgaagaac tcgagccgga ccgaccgttc gccgagaacg atggcacgat gtatgtcggc
     4021 gacaaaggcg tcatgctgcg cgaccggctg atccccgaca agaaatggaa cgagttcggt
     4081 cgggcaccgc agaagctcgc gcggtctccc gggcattggc aggaatggct cgacgcctgc
     4141 cggggcggca aacccgccgg gtcg
//
LOCUS       DATCGS010000089         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_7780, whole genome shotgun sequence.
ACCESSION   DATCGS010000089 DATCGS010000000
VERSION     DATCGS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7780"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VIL39_01760"
     CDS             complement(<1..586)
                     /locus_tag="VIL39_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480185.1"
                     /translation="MNSNDIELLRRYVFERSEAAFADLVRQHIALVYAAALRQTNGDA
                     PLAEDVTQVVFTDLARKAARLTRHTSLAGWLYTSTGYAAAGLRRAEQRRSAREQEAHA
                     MNQLLQSAESDPAWEQLRPVIDEAMHDLKSDDREAVLLRYFERLPLAAVGARLGVTEN
                     TARMRVDRALDKLRGALVKRGVTSTAGALAVILTG"
     gene            complement(770..2980)
                     /locus_tag="VIL39_01765"
     CDS             complement(770..2980)
                     /locus_tag="VIL39_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480186.1"
                     /translation="MYLERLLPENVRHLAPVEQVFSDDLQQIRRWTLLSAGAAATSRL
                     RCLALVSLGRWQMSRLAGRVLPLLAAKPDRPVQMEFVLIRHAPQERQPHGPSRHHQGW
                     RFLAGGRWEALARQALRCQVPGARQGVVESGRSQTGRLLLGYGRSIQAGADGFGLHPD
                     QRWRRCVGLFESESRVTDPVAFLKRLRHKGLYRAGRARAVLDRLKNGLSDWLGWDVAQ
                     SLERGQLEVQWKSTPRARQVPAMVALDIARHAFDAAGRLGESNPLRQPGVVLFERMEA
                     WCPAVKLPAFVRLLDGWFPNLQFILALDAAARRRFPRGLLPQRLPIPEPQPRPVPVAP
                     QRLPRGAVLLVDLDSRLPNLALMKLSRHFKAQGRAVGLHHGIKSLPPVEMVLASCVFN
                     TPVSARRVEVLRRHYGSALQLGGSGVDLRLRLEPEIEALAPDYSLYPELGDRALGFLT
                     RGCPFHCPFCVVPIKEGAPRQVSDLDGLLQGRKKLILLDDNLLAHPDALPLIEDMLRR
                     GLAVNFNQTLDLRLLTSESAALLRRLHCANSAFTRRNLYFSLNDTRHLELMRERYTLL
                     QARPKNNVAFVCMYGFNTTLAEDVARFRFLRSLPGAYVFVQRYLPVLGGPLPDHRRLF
                     DDRADALLKELVRIVFRQNMKSMETYYRWLAIQYAVRCGRIHAELIDTLFRYNGRPRM
                     GRFVAGLEALCQREPDAGDSLAAEGTRETWAGPNRVLSWNNSVKPQMDTDEHGF"
     gene            3326..>3641
                     /locus_tag="VIL39_01770"
     CDS             3326..>3641
                     /locus_tag="VIL39_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY5480187.1"
                     /translation="MNSASTAGAEDSGARFVTTHWSVVLVAGRNDSTRAQNALARLCQ
                     TYWYPLYAYVRRRSHSPHDAQDLTQEFFARLLERHTLAIADPNRGRMPIRFVDKSRKL
                     GSQ"
BASE COUNT          696 a         1233 c         1112 g          600 t
ORIGIN      
        1 ggcccgtcag aatcaccgcc aaggctccgg cggttgaggt cacgccgcgt ttgaccagcg
       61 cgccgcggag cttatccagc gcccggtcca cgcgcatccg cgctgtgttc tccgtgacgc
      121 ccaatcgtgc ccccacggcc gcgagcggca ggcgctcaaa ataccgcagc agcaccgcct
      181 cgcggtcgtc cgacttcaaa tcgtgcatgg cctcatcaat gacgggacgc aactgttccc
      241 aggccggatc gctttcggcg gattgcaaga gttggttcat ggcatgggct tcctgttcgc
      301 gggcgctgcg ccgctgttca gcacgacgaa gacctgccgc ggcgtagccc gtgctcgtat
      361 agagccaccc cgcgagggaa gtgtgccggg tcaggcgcgc cgctttgcgc gccaggtcgg
      421 tgaacaccac ctgcgtgacg tcttcggcca ggggcgcatc gccattggtc tgccgcaagg
      481 cggcggcata gaccagcgcg atgtgttgcc gaaccagatc ggcgaaggcc gcctcggaac
      541 gttcaaagac gtaacgtcgg agcagttcga tgtcgttgct gttcattaca tctgaataac
      601 agccgccggg tggcccaacc gcacaagctt taccaaagcc atgcagaaag taaccacaga
      661 tggacacaga tgaacacaga tagggaatgc ccaacggtaa tcttccatag tgagaatccg
      721 ttcaccggag tgacccagag caaagcgaag caagaccgga gttcggccct cagaatccgt
      781 gttcatctgt gtccatctgt ggttttactg aattgttcca gcttaggact cgattgggtc
      841 cggcccacgt ttcccgcgtc ccctctgcgg ccagactgtc gccagcgtct ggctctcgct
      901 ggcacaaggc ttccagcccc gccacgaatc tacccatgcg cggacggccg ttgtagcgga
      961 acagcgtgtc gatcagctcc gcatggatgc gcccgcaccg caccgcgtat tggatcgcca
     1021 gccagcgata gtaggtctcc atgctcttca tgttctgacg gaagacaatc cggaccagct
     1081 ccttcagcaa cgcgtccgcc cggtcgtcga acaggcggcg gtgatccggc aggggaccgc
     1141 ccagcacggg caggtagcgc tgcacgaaca cataagcgcc cggcaacgaa cgcaggaagc
     1201 ggaaccgcgc cacgtcctcc gccagcgtgg tgttgaagcc atacatgcac acaaacgcca
     1261 cgttgttctt gggccgcgct tgcagcaggg tgtagcgctc ccgcatcagc tcgagatggc
     1321 gggtgtcgtt caggctgaaa tacaggttgc gccgggtgaa cgcggaattg gcgcaatgca
     1381 gccgccgcag caacgccgct gactcggatg tcaacagtcg caagtccagt gtctggttga
     1441 agttcacggc caggccacgc ctcagcatat cctcgatcag cggcagcgcg tcgggatggg
     1501 ccagcaggtt gtcatcgagc aggatcagct tcttgcggcc ttgcagcaga ccgtccaggt
     1561 cgctcacctg gcggggggcg ccttccttga tcggcaccac gcagaatgga caatgaaacg
     1621 gacagccgcg cgtcagaaag cccagggccc ggtcgcccag ctccgggtag agggaatagt
     1681 cgggcgccag cgcctcgatc tccggctcca ggcgcagacg caggtccaca cccgacccgc
     1741 cgagctgcaa cgcggacccg taatgccgcc gcaacacctc gacgcgccgt gccgaaacgg
     1801 gcgtattgaa gacgcaactg gccagcacca tctcgaccgg tggcagtgac ttgatgccgt
     1861 gatgcaagcc cacggcgcgg ccctgcgcct tgaagtgacg actaagcttc atcaaagcca
     1921 ggttgggaag ccggctgtcc aggtccacca gcagcaccgc tccgcggggc agccgttggg
     1981 gcgcgaccgg caccggacgc ggctgcggtt cggggatggg caggcgctgc ggcagcagac
     2041 ctcgcgggaa ccggcgtcgg gcggctgcgt ccagggccag gatgaattga aggttgggaa
     2101 accagccgtc cagcaaccgg acgaaggccg gcagtttcac tgccgggcac cacgcctcca
     2161 tcctctcaaa cagcaccacg ccaggctgtc ggagcggatt gctctccccg agccggccgg
     2221 cggcatcgaa cgcatggcgc gcgatgtcga gcgccaccat cgctggcacc tgacgagccc
     2281 gaggggtgga cttccactga acctcgagtt ggccccgctc caggctctgc gccacgtccc
     2341 agccgagcca gtcgctcaag ccattcttta accggtccag caccgcccgg gcgcgtccgg
     2401 cccggtatag gcccttgtgg cgcagccgtt tcaggaacgc caccgggtcc gtgactcttg
     2461 actccgattc gaacagccca acgcatcgtc tccatcgctg atcgggatgc aggccaaagc
     2521 cgtccgcgcc cgcctggatg gagcgcccat accccaacag cagccgtccc gtctgggacc
     2581 ggccagactc cacaactcct tgccgagcgc caggcacttg gcagcgcaac gcctgacgcg
     2641 ccagcgcttc ccatctccct ccggccagga agcgccagcc ctgatggtgc cgtgacgggc
     2701 cgtgaggctg ccgctcctgc ggcgcgtgcc ggatcaatac gaattccatc tgcacgggcc
     2761 gatccggctt cgccgcgagc aggggcagca cccgtcccgc caatcgggac atttgccacc
     2821 ggcccaggct gaccagagcc aaacagcgca accgcgacgt ggcagccgct ccagcgctca
     2881 gcagcgtcca gcgacggatc tgttgcaggt catcggagaa cacctgctcg accggcgcca
     2941 ggtggcggac gttctcgggc aataatcgct caaggtacat ggctgtcact aacggcgcat
     3001 tcgcctgcta gccaaggagc acggtctcaa agtgccgcgc cagcagccct tcgaactgtt
     3061 tcgcagtctg gttcatggtt taagcaacgc acgaaaagac cccgcttgca cgcaagaatt
     3121 ggggctctct cataaagacc acgtcaggca gcctcgactg cgggaaaggt gatcatggcg
     3181 cgggcggatg gatgggcatg cggggcgggg cggttttcca cagcagctca atctctgccg
     3241 gggtcaggcc gtacgcccct gacccatcaa cccgtgtgat ccacctcgac acctctttat
     3301 gctctgccta tgctccccgg tcagaatgaa ctctgccagc acagcgggag cggaagattc
     3361 gggcgcgcgg tttgtcacca cgcattggtc ggtcgtgctg gtggcggggc gtaatgattc
     3421 cactcgtgcg cagaacgccc tggcccggct ttgccagact tactggtatc ccctctacgc
     3481 ctatgtgcgc cggcgcagcc actcgcccca cgacgcccag gacctgacgc aggaattctt
     3541 cgcgcgcctc ctggaacgcc acaccctggc catcgccgac cccaaccggg gccgcatgcc
     3601 tatccggttt gttgacaaaa gccgaaaact tggaagccag c
//
LOCUS       DATCGS010000090         4380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_7781, whole genome shotgun sequence.
ACCESSION   DATCGS010000090 DATCGS010000000
VERSION     DATCGS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7781"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..864
                     /locus_tag="VIL39_01775"
                     /pseudo
     CDS             1..864
                     /locus_tag="VIL39_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709673.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1160..2674
                     /locus_tag="VIL39_01780"
     CDS             1160..2674
                     /locus_tag="VIL39_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5480188.1"
                     /translation="MRARDVQLYRACDDLTEPLIYFMAVIGPWAFGTTQSWSIWLMNG
                     AGCLLGLLLAGKLAIRWLKGYHPPRWDDVEGGVSGQRAEGRSQRGVAASRLTAILAGL
                     TVAILGYCFVSALNARATAHREEGSFAYHSCIQWLPHSLDSSRSWPAFWTYLGLAFAF
                     WAVRDWLLGKSESEELAERRKPQPGDRRSGFLLPARLRRLLWVLALNGGLLGLEGIVQ
                     RLEGSGKLLFLVKPRVNPGADTQFGPYAYRANASQYFNLLWPVCLGFWWTLNRAYGSK
                     RKAHHLILLGCVIMAACPIISTSRGGALVTVGIGVLAAFFLLATHFLLAAHRQQDRRT
                     RTITLACLLLFFTGALALGFGLGWKTLKPRMAQLSEGFDDREGIYDRARPMAVDYPLF
                     GTGPGTFESVFQLYRKTTDTYWPAQLHNDWLETRITFGWLGSGLIALAFVVVVLRWFA
                     RGGIHGGRRFTVLIWLALAGCLVHARYDFPFQIHSIVFLFLVVCAALSNLTRRP"
     gene            complement(2786..4186)
                     /locus_tag="VIL39_01785"
     CDS             complement(2786..4186)
                     /locus_tag="VIL39_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480189.1"
                     /translation="MEPMNETSNPLSTRREFIKTTGRLAAVSALAGVAIPHVHAASGG
                     LIQLALVGCGGRGTGAANNALSSNGGPMKLVAMADVFDGRLNDSYENIKRAAGDKIDV
                     PQDRKFIGFDAYKKAMDCLKPGDVVILTTPPAFRWVQFTYAIAKGLNVFMEKPVTVDG
                     PTSRRMLKLAEEATAKNLKVGVGLMSRHSRALQELFQRIQDGEIGDVIFLRGYRMHGP
                     GGSAFVTKWPGKPSELLWQIRNFHAFLWASGGCYSDFYIHHVDHLCWMKNAWPVKAQA
                     VGGRHYKNSAEGQPCVDQNFDAYSVEYTFADGAKMYMDGRCINGCNDIYSSYAHGSKG
                     MAIVAKANDCGAPSMTFKGQAMDRSKLLWTSKVAPGQEDPYLNEWNDLMDAIRNDKPY
                     NEAERGVKASLVSSMGRMAAHTGQEITFEEMLNCEHEFAPEVDKLTMESPAPVQAAKD
                     GKYPAPMPGITRKREY"
BASE COUNT          791 a         1310 c         1315 g          964 t
ORIGIN      
        1 ggcgtgattt tcacctggca ttttcctccg agcgcgaaga tcccggtaat cccgacagca
       61 aagtcgcgct catccccaag gtcgtcggtg gctacacgcc ggcctgcctg gaaaaggcca
      121 tcgcgctcta ttccaaggcg atcaagacgg tcgtgccggt gtcgtcctgt cgcgccgccg
      181 aggccacgaa gctgctcgaa aacacctttc gcagtgtaaa catcgccctc gtgaacgaac
      241 tcaaggttgt ctatggcgcc atggggatcg acgtctgggg gggtgatcaa cgcggcgaag
      301 accaagccat tcggctttat gcccttctac ccggggccgg ggttgggtgg gcattgcatc
      361 ccgatcgatc ctgtctacct gacctggaaa gcgcgcgaat ttggccagaa cacccggttt
      421 atcgagttgg ccggcgagat caacacctcc atgcctctct atgtcgtgca ccgggtggcg
      481 gacgcgctca acgatcggca gagggccata aagggcagtt ccattctcgt tcttgggctc
      541 gcctacaagc ccaatgtgga cgacgatcgc gagtcgccga gctatgtcct catggagatg
      601 cttaaagcgc gcggggccaa agtcgcctac tacgaccctt atgttcccgt gatcaaaccg
      661 acccgcgaac atgcgcattg ggctggcacg aagtcagtgc catggaacaa agaaacgatc
      721 accaccttcg acctggtgct cattgctacc aaccacgcct gcgtcaatta ccaggaatta
      781 gcgaattgga cggaatgcat cgtggatacg cgcaacgcga tggctgccgt ccccaccttg
      841 cccaaccagg tcttgaaagc ctgatagcac ttcgttcagc ttaacctcct ccgcttggag
      901 gaagacgtag ccgcccgctg ggcggctgtt tttgttgcaa gcggtctggg ggggcgctgc
      961 cgctccggtt ttccaggaac tcaaactcac tgcctgcttg gctcccgccc gttccagact
     1021 tcaggcttgt taaacgtcgc tgactggact tcaatcgtag cggatggaag ccggtggaca
     1081 gtctcaaatt cccgcccaat gcacacgcaa cgctgctgca gccagcagcc ttatccctcg
     1141 cgtcaggaaa cgtctcccct tgcgcgctcg cgacgtgcaa ctctaccgcg cgtgcgatga
     1201 cctcacggaa ccgctgattt acttcatggc ggtgattgga ccatgggcct tcggcacgac
     1261 ccagtcctgg tcaatctggc tgatgaacgg ggcgggttgc cttttaggtt tgttgctcgc
     1321 cggcaagttg gccattcgct ggctcaaggg ttatcatccg cctcgctggg acgatgtgga
     1381 agggggagtc agcgggcaga gggcagaggg cagaagtcag cgaggggtgg cggcgtcgcg
     1441 cctcaccgcc atcctggccg ggctgacggt tgcgattctg gggtattgct tcgtcagcgc
     1501 cttaaacgcg cgggctaccg ctcaccgcga ggaagggagc ttcgcctatc attcctgcat
     1561 ccagtggttg ccccatagcc tcgatagcag ccgaagttgg ccggcctttt ggacttatct
     1621 tgggctggcg tttgcatttt gggctgtgcg cgattggttg ctgggcaagt cggagtccga
     1681 ggaactggct gaacgccgca agccgcagcc cggcgaccgc cgctccggct ttttgctccc
     1741 cgctcgcttg aggcgactgt tatgggtgtt ggctctcaac ggtgggttgc tagggctgga
     1801 aggcatcgtt cagcggctgg aaggctccgg caagctgctt tttctcgtaa aaccgcgcgt
     1861 caacccgggc gcagacaccc agttcggtcc ctatgcctac cgcgctaatg cgtcgcaata
     1921 tttcaatctc ctttggcccg tatgcctggg gttttggtgg acactcaacc gagcgtatgg
     1981 ctctaaacgc aaagcacatc atctgatcct cctgggctgc gtcatcatgg cggcctgtcc
     2041 gattatttcc accagtcgtg gcggcgcgct cgtcacggtc gggattggcg ttctggctgc
     2101 cttctttttg ctcgccaccc atttcctgct ggccgctcac cggcagcaag atcgccggac
     2161 gcggacgatt accctggcct gcctattgct gtttttcact ggcgcgctgg cgctcggctt
     2221 tgggcttggc tggaagacgc tgaagccgcg catggcccaa cttagtgaag gcttcgacga
     2281 ccgcgaggga atatatgatc gggcccgccc gatggccgta gattatccgc tattcggcac
     2341 tggacccggc actttcgagt cggtctttca gctctatcgc aagaccacgg atacctattg
     2401 gccggcgcaa ttacataacg actggctgga aacccgcatc acatttggct ggctcggcag
     2461 cgggttgatc gccctggcct ttgttgtcgt ggtgctgcgc tggttcgcgc gcggtggcat
     2521 tcacgggggc aggcgcttca ctgttctcat ctggctggca ttggcaggct gtctcgtcca
     2581 cgctcgctac gactttccct tccagattca ctccatcgtc tttctgttcc ttgtcgtttg
     2641 tgcggccctg tctaatctca cccgccggcc atagtgtggc gaggggcaag aatcaacagc
     2701 gcttgtccct ggcgatggca gccaccgcag aaggccaggt tgtggccgac cgccgcagca
     2761 actgaagggc aacggggcga aggaattagt actcccgctt ccgggtgatg cccggcatcg
     2821 gggcaggata cttgccatct ttggcggctt gcaccggggc cggggactcc atcgtgagct
     2881 tgtccacctc gggggcgaac tcgtgctcgc agttgagcat ttcctcaaag gtaatctcct
     2941 ggccggtgtg ggcggccata cggcccatgc tggagacgag gctggctttg acgccgcgtt
     3001 cggcctcgtt gtagggcttg tcattgcgga tggcgtccat cagatcgttc cattcgttca
     3061 gatagggatc ctcctggccc ggggcgactt tggaggtcca aagcagtttg gaacgatcca
     3121 tagcctggcc tttgaaggtc atggagggag cgccgcagtc gttggccttg gcgacgatgg
     3181 ccatgccttt gctgccatgc gcgtagctgg aataaatgtc gttgcagcca ttgatgcagc
     3241 ggccatccat gtacatcttg gcgccgtcgg caaacgtgta ttccacggaa tacgcatcga
     3301 aattctgatc cacgcaaggt tggccttccg cgctgttctt gtaatgacgc ccgcccaccg
     3361 cctgggcttt gaccggccac gcgtttttca tccaacagag gtgatctaca tgatgaatgt
     3421 aaaagtcgct gtaacatccg ccgctggccc acaggaaggc gtggaagttc cgaatctgcc
     3481 agagcaactc gctgggtttg cccggccact tggttacaaa ggccgacccg ccagggccgt
     3541 gcatccggta accgcgcagg aagataacgt cgccgatctc gccgtcctgg atgcgctgaa
     3601 agagttcctg cagcgcccgg ctgtgacggg acatcagtcc caccccgacc ttcagattct
     3661 tggccgtcgc ctcttccgcc aacttgagca tgcggcggct ggtcgggccg tccaccgtga
     3721 ccggcttttc catgaacacg ttcagcccct tcgcgatggc gtaagtgaac tggacccagc
     3781 ggaacgcggg cggagtggtc aggatcacaa cgtctcccgg cttgaggcag tccattgcct
     3841 tcttataggc gtcgaagccg atgaacttgc ggtcctgcgg cacatctatt ttgtcgccgg
     3901 ccgcccgctt gatgttttcg tagctgtcgt tgagcctccc gtcgaagacg tcggccatgg
     3961 cgacgagctt catggggccg ccgttgctgg agagcgcatt gttcgccgcg ccggttccgc
     4021 ggcccccgca gcccaccagc gccagttgaa tgaggccgcc gctggcagca tggacatggg
     4081 ggatggcgac tcctgccagc gcggaaacgg cggcaaggcg gcccgtggtt ttgataaact
     4141 cgcgacgtgt agagagagga ttagacgttt cgttcatggg ctccatattc cagacgaaga
     4201 tggagaaatg tcaactcaag aaatcaagaa atcagcggga agatcagcgg ggcgaaactg
     4261 cgttaataga gggcgagttc tcaagccggt ggtgttcaaa cgggtgtgct tgactccggc
     4321 gctacattcc agtccacttt gcacccatgc gctgcatatt taaccggcga atcagttcgc
//
LOCUS       DATCGS010000091         4989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_354062, whole genome shotgun sequence.
ACCESSION   DATCGS010000091 DATCGS010000000
VERSION     DATCGS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354062"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /gene="recC"
                     /locus_tag="VIL39_01790"
     CDS             <1..2424
                     /gene="recC"
                     /locus_tag="VIL39_01790"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEY5480190.1"
                     /translation="RDFLNLVLEAGDWDEARAFSEPPETNLLQHIQADLFHLHDRGQG
                     DCPRLAIGRTDASLRVHSCHSPLREVEVLYDHLLDWLEHDPKLTPRDVLVMTPDIEAY
                     APFIQAVFDSPEESSKRIPFSVADRGISSASQVVDAFLNLLSLPSTRLEATSVLRILE
                     TNAVREKFGLAESDLDIIRVWVRGTNIRWGQDAKQRESLGLPGLAENTWEQGMERLFA
                     GYAMAGKGVKMFQGVLPYDDVEGGSAEALGRFEEYLNRLFHLVAELKERRTIGEWEEI
                     LLGALEAFFQPHESQVSETLLIRSALRELASKAAEADCAEPVDLEVILESLNQLLAED
                     RFGSGFITGGVTFCALKPMRSIPFKVICLIGMNDGVFPRTDRHLSFDLMAQKPRLGDR
                     SLRADDRYLFLETLLSARERLHISYVGQSIRDNSEAPPSVLVSELLDYVAQAYELPGQ
                     DILKDHVLVPHRLQAFSPAYFTAHDGRLFSYSAENCRASHYGQASRVMPASFLDVPLS
                     EPGSEWRTVDAGVLAEFFCNPARWLVTRRLGLRFEEQDEALEEVEPFEVAGLDGYAIR
                     QDLVSMGLKGTSPKDALQLMKASGRLPLGETGAALFRGLQAEVQTFLEQLRPHFGEGY
                     IDPIPVDHTLGEFRLVGEIKRLTAKGSLHFRCAGIKPKDLLRFWVQHLVLNAAFPNGQ
                     YSSAVLVGSDKVFEAPPLVRAPEFLAGLLDLYWKGLTRPLRFFPQTALAYADAVLKQE
                     AGRSKQEPIEVARLSWEGSSFRKTPGECEDAYFDLCFRGVDPLDEEFEQTARAVFEPL
                     LTELKEAGA"
     gene            2421..>4989
                     /locus_tag="VIL39_01795"
     CDS             2421..>4989
                     /locus_tag="VIL39_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5480191.1"
                     /translation="MKPVPVFQLAETELRTGTSLVEASAGTGKTFTIAGLFLRLILEK
                     NLSVREILVVTYTVAATEELRHRIRHTLAKALQAFTTGASDDPFLRALLTRQADQRAD
                     LAARLDRALYGFDEAPIYTIHGFCQRVLRDRAFETGNLFDTELVTDQTPLLRQLVEDY
                     WRKQFYRAGKIPVIFALKNGLSPEGLLPLVRTSLPHPFLKLLSPVDGKTPESLAAALE
                     AAFVSLREVWREQKDAIRSHFGSGAKWANKPYNDDEAMADAFRQLDSCLDAPEFPPSA
                     LDALQLFRKSAIAAKVSKRAKGPAPAHPFFDLCDELSRAEEHFVIGVKLGALDYVRQE
                     LPRRKDELKIQFFDDLLTRLHGALAGTGGLGLATLLRRQYVAALIDEFQDTDPLQYDI
                     FSRVFAGPENYLFLIGDPKQAIYGFRGADIFTYLKARQHSDQAYTLKENWRSEAGLVR
                     SVNAVFSAAATPFVFPGIQFTAVEAKGEADKKPLKLDGQSQPPFQFWFWRRTGANINK
                     GDADKLLPSVVASEIVELLNGHTALGDRKLLPEDIAVLVPENRRAQLVQDALSRRNVP
                     SVLYTSASLFDSREVVETQRVLAAIADPTHESQLMAALATDLVGFTGSRIEAEVKQET
                     EWQQVLERFRGYLDLWLQRGFIQMFRSFMQREQVRPRLLAFPDGERRLTNLLHLSEVL
                     HRASVENRLGVSGLIKWLGEQREPGDQVAEEHQLRLETDEKAVKLVTIHKSKGLEYSV
                     VFCPFSWKGANIEHGGQEQVFFHEQGDGNLVRDLGSPDYDANKQQARVERLAENVRLL
                     YVALTRAKHRCYFVWGAFRDAATSAPAWLLHPPPNPEPDPVTAQEEHFPQLDDDQLLD
                     "
BASE COUNT          902 a         1604 c         1554 g          929 t
ORIGIN      
        1 cgcgacttcc tcaacctggt tcttgaggct ggcgactggg acgaggctcg cgccttttca
       61 gagccgcccg agacaaacct gctgcagcac atccaggccg acctgtttca cctgcacgat
      121 cgggggcagg gggactgccc cagacttgcg attggccgga cggacgcgtc gctgcgggtg
      181 cattcctgtc acagcccctt gcgcgaggtg gaagtgctct acgaccactt gctggattgg
      241 cttgagcacg acccgaagct gacaccccgc gacgtgctcg tgatgacgcc ggatatcgag
      301 gcctacgcgc ctttcatcca ggcggtgttc gactcgcccg aagagagcag caagcgaatt
      361 ccgttcagtg tagccgaccg gggcatctcc agcgccagcc aggtcgttga tgcgttcttg
      421 aacctgctca gcctgccttc gacgcgcctg gaagcgacca gcgtcctgcg cattctggaa
      481 accaacgcgg tgcgggagaa gtttggtctg gccgagtccg accttgacat catccgggtc
      541 tgggttcgcg ggaccaacat ccgctggggc caggacgcga agcagcgcga gagcctgggg
      601 ttgcccggct tggcggagaa cacgtgggag cagggcatgg agcggctgtt cgcgggctat
      661 gctatggccg gcaaaggcgt aaagatgttt cagggcgtcc tgccgtatga cgatgtggaa
      721 ggcgggagtg ccgaggcgct gggtcgcttt gaggaatatc tgaaccggct gttccacctc
      781 gtggctgaac tcaaggaacg gcgaaccatc ggggagtggg aggaaatcct gctcggcgcg
      841 ctggaagcgt tcttccaacc ccatgaaagc caggtgtcgg aaacactgct tatccgctcg
      901 gcgctgcggg agcttgccag caaggcagcc gaggccgatt gcgcggagcc ggttgacctc
      961 gaggtgatcc ttgagtccct gaaccaactc ctggcggaag accgcttcgg ctccggcttc
     1021 atcaccggtg gggtcacgtt ctgtgctctc aagcccatgc gcagcatccc cttcaaggtg
     1081 atctgcctca ttggcatgaa cgacggcgtc ttcccacgga ctgatcggca cctgagcttt
     1141 gacctgatgg cgcagaagcc gcggttgggc gaccgctccc tgcgggcgga tgaccgttat
     1201 ctcttcctcg agacactgct ttcggcgcgc gagcggctgc acatcagcta cgtcggtcaa
     1261 tccatccgcg acaacagcga agccccgccg tcggtgctgg tgagcgaact gctggattac
     1321 gttgcccagg cttacgaatt gccgggccag gacattctca aggatcacgt gctggtgccg
     1381 catcgtctgc aggcgttcag cccggcctac ttcacggcgc acgatgggcg gctcttcagt
     1441 tactcggccg agaactgccg ggccagccat tacgggcagg cctcacgggt tatgccggct
     1501 tccttccttg atgtgccgct gagcgaaccc ggctctgagt ggcgcacggt ggacgcaggg
     1561 gtgctcgccg agttcttctg caaccccgcc cgctggctgg tgacccgccg gctggggctg
     1621 cgattcgagg agcaagacga ggccctggag gaagtggagc ccttcgaagt ggctggcttg
     1681 gacggttacg ccatccggca ggacctcgtg agcatgggcc tcaaagggac cagtcccaaa
     1741 gacgccttgc agttgatgaa ggcatcgggc cggctgcccc tgggcgaaac cggagctgcg
     1801 ctcttccgcg ggttgcaagc cgaggtgcag acgttccttg aacaactgcg gcctcacttc
     1861 ggtgagggtt acattgaccc cataccggtt gaccacacgc tgggcgagtt tcgcctggtg
     1921 ggagagatta agcggctgac agccaaaggc tcgctgcact ttcgctgcgc gggcatcaag
     1981 cccaaggacc tcttgcgctt ttgggtccag cacctcgtcc tgaacgccgc gtttcccaac
     2041 gggcaatact caagcgccgt gctcgtgggc agcgacaaag tgtttgaagc tccgccactc
     2101 gttcgggcgc ccgagtttct ggccggtctg ctcgatctct attggaaagg cctgaccagg
     2161 ccgctgaggt tcttcccgca gaccgccttg gcttatgcgg acgccgtctt gaagcaagag
     2221 gctgggcggt caaagcagga gccgatcgag gttgcccggc tcagttggga gggcagctcg
     2281 ttcaggaaga cgccaggcga atgcgaggac gcttactttg atctgtgttt tcgtggcgtt
     2341 gacccccttg acgaagagtt cgagcaaacg gcgcgcgcag tcttcgagcc gctgttgacc
     2401 gagctgaagg aggcgggagc atgaaacctg ttcccgtctt ccagttggcc gagacggagt
     2461 tacggaccgg caccagcctg gtcgaggcca gcgccggcac cggcaagaca ttcaccatcg
     2521 ccggtctctt cctgcggctc atcctggaga agaacctgtc cgtccgcgaa atcctggtgg
     2581 tgacctacac ggtggccgcg acggaggaat tgcgccatcg catccgccat actctggcca
     2641 aagccctgca ggccttcacc accggtgcga gcgacgaccc cttcctccgt gccttgttga
     2701 ccaggcaggc ggaccagagg gctgatctgg ccgcgcggtt agaccgtgcg ctctacggct
     2761 ttgatgaggc gcccatctat accatccatg gcttctgcca gcgggtgctc agggaccggg
     2821 cctttgagac cggcaacctg ttcgacacag agttggtcac cgaccagacg ccgctgttgc
     2881 gccaacttgt cgaggattac tggcgcaagc agttctaccg cgcgggcaaa atccccgtca
     2941 tctttgccct gaagaacggc ttgagccccg aaggcctcct gcccctggta cgaaccagcc
     3001 tgcctcaccc gttcctcaag ctgctttcac ccgtggacgg caagactcca gaatccttgg
     3061 ccgcagccct ggaggctgcc ttcgtttctc tgcgcgaagt ctggcgcgag cagaaggacg
     3121 ccatccgcag ccactttggc agcggcgcca agtgggcgaa caagccttac aacgatgacg
     3181 aggcgatggc ggatgccttc cgccagcttg acagttgcct cgacgctcca gagttccccc
     3241 catccgcgct ggacgccttg cagttgttcc gtaagtctgc cattgcggca aaggtgtcca
     3301 agcgggccaa aggcccggcg ccagcgcatc ccttcttcga cctgtgcgat gaactatccc
     3361 gcgcggagga gcacttcgtc atcggcgtca agctcggcgc gctcgattat gttcggcagg
     3421 agctgccgcg ccggaaggac gagcttaaga tccagttctt cgacgacctg ctcacacgcc
     3481 tgcacggcgc cttggccggc accggtggcc ttgggctggc cacactgctg cgcaggcaat
     3541 acgtggcggc cctgatcgac gagttccagg acaccgatcc cttgcagtat gacatcttct
     3601 cgcgcgtgtt cgcggggccg gagaactatc tgttcctgat cggcgatccc aagcaagcca
     3661 tctacggctt ccgcggggcg gacatcttca cctatctgaa agcccgccag cactccgacc
     3721 aggcctacac ccttaaggag aattggcgct cggaagccgg cctggtccgt tcggtcaacg
     3781 ccgtgttcag cgccgcggcc acgccgttcg tgtttcccgg catccaattc actgcggtgg
     3841 aagccaaggg tgaagcggat aagaagcccc tcaaactgga cggccagtcc cagccgccct
     3901 tccagttctg gttctggcgg cggaccgggg ccaacatcaa caagggcgac gccgacaagc
     3961 tgctcccgtc ggttgtggcc agcgagattg tcgagctgct aaatggtcat accgccctgg
     4021 gcgaccgcaa gctgctgccg gaggacattg ctgtcctggt gcccgagaac cgccgggccc
     4081 aattggtcca ggacgcgctc agccggcgca acgtccccag cgtgctctac accagcgcca
     4141 gcctgtttga ctcccgggaa gtggtggaaa cgcagcgcgt gctcgccgcg attgccgacc
     4201 cgacccatga atcccagctc atggccgccc tcgcgacaga cctggtcggc ttcactggtt
     4261 cgcggattga agccgaggtg aagcaggaga cggagtggca gcaagtcctc gaacgcttcc
     4321 gcggctatct ggacctgtgg ctccagcgcg gcttcatcca gatgttccgc agcttcatgc
     4381 aacgcgagca agtccggccc cgtctgctgg ccttccccga tggcgagcgc cggttaacca
     4441 acctcctgca cctctcggaa gtgcttcacc gcgccagcgt cgagaatcgt ttgggggtct
     4501 ccggcctcat taagtggctc ggcgagcagc gcgagccggg agaccaggtc gccgaggagc
     4561 accaactccg gctggagacc gacgagaagg ccgtcaagct ggtcaccatc cacaagagca
     4621 agggcctgga atactcggtc gtcttctgcc ccttctcgtg gaagggcgcg aacatcgagc
     4681 acggcggcca ggagcaggtc ttcttccatg agcagggcga cggcaacctc gttcgcgacc
     4741 tcggcagccc tgattacgat gcgaacaaac agcaagcccg agtcgaacgc ctggctgaaa
     4801 atgtccgcct cctatacgtg gcactgacgc gcgccaagca tcgctgttac tttgtctggg
     4861 gcgctttccg cgacgccgcg acttcggctc ccgcctggct gctgcatccg ccgccaaatc
     4921 ccgagcccga cccggtaacg gcgcaggaag agcacttccc tcaactcgac gacgaccaat
     4981 tgctcgacg
//
LOCUS       DATCGS010000092         3713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_601754, whole genome shotgun sequence.
ACCESSION   DATCGS010000092 DATCGS010000000
VERSION     DATCGS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601754"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VIL39_01800"
     CDS             complement(<1..338)
                     /locus_tag="VIL39_01800"
                     /inference="COORDINATES: protein motif:HMM:NF023598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HEY5480192.1"
                     /translation="MNPTPESRSLNLRATPTSDFGSQQLAISNPADLRFGVAPKPLTT
                     RRGLTIGGGIVYPELNFTLPPMFVDQTTMPEVRVQYQQIITGALRRAVELEAPGLVVE
                     FETLPPMTENP"
     gene            complement(552..1445)
                     /locus_tag="VIL39_01805"
     CDS             complement(552..1445)
                     /locus_tag="VIL39_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5480193.1"
                     /translation="MKNRSAMGTGLEGEHQVLLPEPVIKAALRDKCLLRGLLAVGAGY
                     RPRAAGHLRRRPKGVGEALLVYCVKGGGWCELAGQLHTVRAGDLLVLPPDVPHNCGAH
                     VSDPWTIHWAQATGANLHEYLNELGVSAQAPLVWMGDDLQLARLFHEVVKTLEQGSSF
                     LHLLEASHALAHLLAVMIRHRHEHSRDTSDTVQKVAEGIIYMSEHLDQPMRVSALAAL
                     ANLSPAHFSVLFKEQTGCSPRDYLHLLRIHRACQLLRTSNLSVKEIATRLGYQDQFHF
                     SRQFKAFQGASPSDYRESLSV"
     gene            complement(1474..2937)
                     /locus_tag="VIL39_01810"
     CDS             complement(1474..2937)
                     /locus_tag="VIL39_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEY5480194.1"
                     /translation="MASNQSYNVKFFSGEEVPLELHKVRIVQKLNLIPIEERLKAIVE
                     GGFNTFLLTTKTVFLDMLTDSGTNAMSDNQVASMLRADDAYAGSQSYMRLEEAIQQVF
                     NCKYFLPAHQGRAAENIISRVFIKPGDVVPMNYHFTTTKAHIELNGGTVLEIYTDEAL
                     KVKSKHPFKGNMDIAKLEQIIKKYGASKVAFIRLEASTNLIGGQPFSIANMRAARKVA
                     KKHGIMVVLDASLIGENAYFIKQRETEFKNKSIKQILHEMIGLVDLTYFSSRKVSSTR
                     GGGICTNNKEIHNKMKDLVPLFEGFYTYGGMSIREVEAMAIGLVETLDETVISQSPKF
                     IRYMVNELERLGVPVVTPAGGLGVHIDAGQFLAHVPQAEYPAGALAAAFFLISGVRGM
                     ERGTISSVRDANGNDVLADIELLRLAMPRRVFTLSQVKYAIDRLYWLYKNRKLVGGLK
                     FYEEPPVLRFFGGKLKPTSPWPQKLVVKFRADFGDSL"
     gene            3213..3617
                     /locus_tag="VIL39_01815"
     CDS             3213..3617
                     /locus_tag="VIL39_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480195.1"
                     /translation="MPEIVAFVHRGAASITPAMAEKVIRHLPQWKLEFTQIYEPLFPH
                     LVDQLEFLADAVEDTTEGAYKELPYIAFAQSLFALVYAHKKVGIIPDSVLNLGRADDS
                     SVVRAVLIQNEKAFAVYAASQNVEWSRITSQP"
BASE COUNT          714 a         1103 c         1140 g          756 t
ORIGIN      
        1 gggttctcgg tcatcggcgg cagcgtctcg aactcgacca ccaggccggg cgcctcgagt
       61 tccaccgccc gccgcaaggc gccggtgatg atctgctggt actgcacccg cacctccggc
      121 atggtggttt ggtccacgaa catgggcggc agggtgaagt tcagttcggg atagacgatc
      181 ccgccgccga tggtcagccc ccggcgggtg gtgaggggtt tgggcgcgac gccgaagcgc
      241 aggtcggcgg gattgctgat ggcgagctgc tgggagccga agtccgaagt tggagttgcc
      301 cggagattca gggagcgaga ctcgggagtt gggttcacgg gaggcaagtt gtgtctaacc
      361 gtgtctgctt tgtgcatcat gtgggatcag gatagcccac ccttggacaa aaggggaatg
      421 gatgaatcaa ggattggcat gaaggtatcg cggatgggcc ttaagcgggg agggctgcgc
      481 ggggctcaaa gccacgagga ccgggcgttc ggtcaaagtc ccgcgttacg gcgcacatga
      541 agccagccgc ctcaaacgct caacgattcc cggtaatcgc tcggggaggc gccctgaaac
      601 gccttgaact ggcgcgagaa atggaactgg tcctggtagc cgagccgggt ggcaatctcc
      661 ttcacgctca ggttcgaggt gcggagcaac tggcaagcgc ggtgaatgcg cagcaggtgc
      721 aggtaatccc gcggtgagca gccggtctgc tccttgaaga gcacggagaa atgcgccggc
      781 gagagattgg ccaacgcagc cagcgcgctg acgcgcatcg gctgatcgag gtgctcgctc
      841 atgtaaatga tcccctcagc caccttctgg acggtgtccg aggtgtcgcg ggaatgctcg
      901 tggcgatgcc ggatcatcac ggccagcagg tgtgctagcg cgtgcgaggc ttccagcagg
      961 tggaggaatg agctgccctg ctccagggtt ttgaccacct catggaatag ccgggccagt
     1021 tgcagatcat cgcccatcca gaccagcggc gcctgcgcgg agacccccaa ttcgttcagg
     1081 tattcatgga ggttggcacc ggttgcctga gcccagtgga tggtccacgg atcggaaaca
     1141 tgcgcgccgc agttatgcgg cacgtcgggc ggcagcacca gcaggtcgcc ggcccgcacg
     1201 gtgtgtagct gcccggccag ttcgcaccag ccgccgcctt taacgcaata gaccagcagc
     1261 gcctcaccga cgcccttcgg gcggcggcgc agatggccgg cggcacgcgg gcgatacccc
     1321 gccccgacag ccaacaggcc gcgcaacagg catttatccc gcagcgcggc cttaatcacc
     1381 ggttcaggca gcagcacctg gtgctctcct tcgaggccgg tgcccatggc tgagcgattc
     1441 ttcaccgtgt tccggtgttg aatcggatca gcctcacagg ctgtccccaa agtccgcccg
     1501 gaacttaacc acgagctttt gcggccatgg gctggtcggc ttcagcttgc ccccgaaaaa
     1561 gcgcagcacc ggcggctcct catagaattt caggccgccg accagcttcc ggttcttata
     1621 cagccagtac aaccggtcga tggcgtattt gacctgcgag agtgtgaaga cgcggcgcgg
     1681 catcgccagg cggagcagtt cgatgtccgc cagcacgtca ttgccgttgg catcgcgaac
     1741 gctggagatg gtgccgcgct ccatgccgcg aacaccggag atcaggaaga atgccgccgc
     1801 cagcgccccc gcgggatact cggcttgcgg gacatgcgcc aggaactgcc cggcgtcaat
     1861 atgcacgccc aagccgccgg ccggcgtcac caccggcacg cccagccgtt ccagctcgtt
     1921 gaccatgtac cggatgaact tgggcgactg cgagatgact gtctcgtcga gcgtctcgac
     1981 caggccgatc gccatggcct cgacctcgcg gatggacatg ccgccgtagg tgtagaaacc
     2041 ttcgaacagc ggcacgagat ccttcatctt gttgtggatc tccttgttgt tggtgcaaat
     2101 gccgccaccc cgcgtcgagc tgaccttgcg gctggagaag taggtcaggt caacgaggcc
     2161 gatcatctcg tgcaggatct gcttgattga tttgttcttg aactcggtct cgcgctgttt
     2221 gatgaagtag gcattctcgc cgatcaagct ggcatccagc acaaccataa tgccgtgctt
     2281 cttggccacc ttcctggccg cgcgcatatt ggcgatcgag aacggctgcc cgccgatcag
     2341 gttggtcgag gcttccagtc gaataaaggc gaccttgctc gcgccgtact tcttgatgat
     2401 ctgctccagc ttggcaatgt ccatgttgcc cttgaaagga tgcttgctct tcaccttcag
     2461 ggcttcgtcg gtgtagatct ccaggacggt gcctccgttg agttcaatgt gcgccttggt
     2521 ggtcgtgaag tggtaattca tcgggaccac atcgcccggt ttgataaaca ccctcgagat
     2581 gatgttctcg gctgcccgcc cctggtgcgc cggcaggaaa tacttgcagt tgaacacttg
     2641 ctgaatggcc tcctccagcc gcatatagct ctgcgagccg gcataggcat catccgcccg
     2701 cagcatcgac gccacctggt tgtcgctcat tgcgttggtg ccgctgtcgg tgagcatatc
     2761 caggaacacc gtcttggtcg ttaaaaggaa ggtgttaaac ccgccctcga cgatggcctt
     2821 caggcgctcc tcgatcggga tcaggttgag cttctgcacg atgcggacct tgtgcagttc
     2881 cagggggact tcttcaccgc tgaagaactt aacgttgtag ctttggttgc ttgccatatt
     2941 aattgctagc tcgattatta atgattctgc atatttggaa attgcgtttc tcactcgcca
     3001 ggtggaagag catggaaacc atgcgcaatt tcgaggccgc caaactaaga gcccggccaa
     3061 ccctaacgca aggccatttt tgcggggctc agcctaacga attcgtggtc acagccgaga
     3121 agcaattccc ggcctccccg gaacccagcc gcgccgagtt ttagcaaata tttgtgtttc
     3181 cctccgggcg agccttcgga tagagtccgc tcatgcctga aattgtggcc tttgtacatc
     3241 gtggggcagc ctcgatcacc ccggctatgg cggagaaggt gatccggcat ttgccgcagt
     3301 ggaaactgga atttacgcag atctatgagc cgctctttcc gcacctcgtt gatcagctcg
     3361 aattcctggc tgacgccgtc gaagacacca cggaaggcgc ctacaaggaa ttgccgtata
     3421 tcgcttttgc ccagtcgctc ttcgcgctcg tttacgccca caagaaagtg ggcatcattc
     3481 ccgattccgt cctgaacctc ggccgcgctg acgattcgag cgtggtgcgg gccgtgctga
     3541 ttcaaaacga gaaggccttt gccgtttacg ccgcgtcgca aaacgtcgag tggtcccgga
     3601 tcacgagcca gccgtaggcc gaggggactg gggcgtcgga gtgatggagt aaaggagtgt
     3661 tggagaactg ggaaacctct ccgatgcctg tccatcaatc caccaatcca tta
//
LOCUS       DATCGS010000093         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_552818, whole genome shotgun sequence.
ACCESSION   DATCGS010000093 DATCGS010000000
VERSION     DATCGS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552818"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1211
                     /locus_tag="VIL39_01820"
     CDS             414..1211
                     /locus_tag="VIL39_01820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5480196.1"
                     /translation="MLNEEANLPSRMNAGVPSYFTSLKPFTGLFATGVPSLMYHKLGR
                     RPWGVRLKGLYLSRSLFERQLSQLHAAGFTTPAYGPPPLSEGNPGRRIVLTFDDGFAN
                     VFEHGLEPLARHGFRAVIFLVADLIGGCNGWEMQEGEARQPLMDETQVKEWLAAGHEI
                     GSHTLTHPFLTRLSLKKAGEEISASKKKLEDCFGRPIRHFCYPYGDWNPAMRDRVVAA
                     GYETACTTEFGVNTKATPPFELKRILVRYQSISFKALKERLARTRFR"
     gene            complement(1281..2072)
                     /locus_tag="VIL39_01825"
     CDS             complement(1281..2072)
                     /locus_tag="VIL39_01825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5480197.1"
                     /translation="MAESKYIENKAYLARCEREHMLALRETGTRHALEFGREDEWLRC
                     VKGDFPKYIPFLKEKCGLEFRGRILEIGAGGAWLSAELSKQPRVVEVIATDLSPTLLK
                     EHAPKVFKLLNANAAKITRMPGDFHKLDFPDNHFDFVVCSAVLHHAANIVQVLREAKR
                     VLKPGGQFVAIREPVWPLVKIKSRAKMLAKLVATGVNERFYTLADYKEFFNQASLPLQ
                     VKRVNLSSGFKYYVNKVVNGLTHARYAFVGKKPGRREKVTFGSRP"
     gene            complement(2148..2633)
                     /gene="tadA"
                     /locus_tag="VIL39_01830"
     CDS             complement(2148..2633)
                     /gene="tadA"
                     /locus_tag="VIL39_01830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY5480198.1"
                     /translation="MSDEPIIDLQSDHYFMGEALRQAAKAYAAEEVPVGAVVVREGRV
                     IARSFNQVELLKDATAHAEMLALTQAEGAVGDWRLTDCTLYVTKEPCPMCAGAIVHVR
                     LARVVFGASDPKAGAAGSALNLLQFPTLNHRCEITPGVREPECRALLRAFFAEQRKQK
                     E"
     gene            complement(2684..>3300)
                     /locus_tag="VIL39_01835"
     CDS             complement(2684..>3300)
                     /locus_tag="VIL39_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5480199.1"
                     /translation="RGPGAVIHCDGAWGIVYPHGEEPVLCQEPRSPRTMKLPYKLATL
                     LYCFNERDEVLLLERAQEPNLGLWSPCGGKLHTDTGESPYACACREAHEELGILVSPS
                     DLHLTGLASEHGYQGQSHWLMFLFEVKRKLTALPPVHREGRFQYFPHEALPGLKIPQT
                     DRERIWPWFWQHRRGFFAAHCHCHPDGRNEWTLEESSVARREGG"
BASE COUNT          685 a         1034 c          942 g          639 t
ORIGIN      
        1 gtgctggcgg caaaagagca taagggtatc agggcgaaaa aaacgcccca gagaagcacc
       61 aggaaaggca cacgcctccc ccgacctgcc attactgaaa tcgtcatttg gagttctgaa
      121 cgcgagaaac gtgccaacgc taacggccgg acctcgctga agtccggtgg ttggcgcagc
      181 ctccccacga aagcgggatg ccaagacgca ttctccccag tatccagcgg gttttggggg
      241 caccccaacg atgagaacca agcctgaatc gcaatactcc gggagcggaa atgattcaaa
      301 ttcttggaca ggttgtccta aaagaaagca ggcgatggct ccacaaaccg ctcctcttga
      361 caccaagggt tgactccacc tgcggtattg caccagcatc ggcggcagtg aatgtgttga
      421 acgaagaagc caatctgcca agccggatga acgccggcgt tccttcgtac tttacctcac
      481 tcaagccctt caccgggttg ttcgcaaccg gggtgccctc gctgatgtat cacaagctgg
      541 gacggcgccc gtggggtgtc cggctcaagg ggctttatct cagccgctcg ctgttcgaac
      601 ggcagttgtc gcaactgcac gcggctggct ttaccacccc cgcctatggc ccgccgccgt
      661 tgtcggaagg caatcccggc agaaggatcg tgctgacgtt tgatgacggg ttcgccaacg
      721 tgtttgagca tggcttggag ccgctcgccc ggcatggatt tcgcgcggtc atattcctgg
      781 tggcggacct gatcggcggc tgcaatggct gggaaatgca ggaaggggag gcgcggcaac
      841 ccctgatgga cgagacccag gtcaaagagt ggctggcggc gggccacgaa atcggatcgc
      901 ataccctcac gcatccgttc ctgacccggc ttagtctgaa gaaggcgggg gaagaaatct
      961 ccgccagcaa gaagaagctc gaagattgct ttggccggcc gatccggcac ttctgctacc
     1021 cttacggaga ctggaatcca gccatgcgcg atcgggtcgt ggcggcgggg tatgaaacag
     1081 cctgcaccac ggagttcggg gtaaacacca aggccacgcc gcccttcgaa cttaagcgca
     1141 tcctggtccg ctatcagtct attagtttca aggcgctcaa ggagcgcctg gcccggacca
     1201 ggttccgatg agcaactcag ccgctgtgcg gtccgagggc cgcaacgcgg gccactggaa
     1261 agaaatgggg ggctctcagc ttacgggcgt gaaccaaagg tcactttctc gcgtcgccct
     1321 ggcttcttgc ccacgaacgc ataccgcgca tgggtcaagc cgttgaccac tttgttgacg
     1381 taatacttga atccgctgga tagattgacg cgcttgacct gcagcggcag agaggcttgg
     1441 ttgaagaact ccttgtagtc agccagcgta tagaagcgct cgttcacacc cgtggcgaca
     1501 agtttggcca gcattttcgc acgcgactta atcttcacca gcggccacac tggctcgcga
     1561 atcgccacga actgccctcc cggcttcaac actcgtttgg cttcgcgcaa tacctgcacg
     1621 atattcgccg cgtggtgcaa cacggcggag cacaccacga agtcgaaatg attgtccggg
     1681 aaatcgagct tatggaagtc gcccggcatg cgcgtgatct tggcggcatt ggcattgagc
     1741 agtttgaaca ccttcggcgc gtgttccttc agcagcgttg gcgataaatc cgtcgcgatg
     1801 acctcgacca ccctgggctg cttcgacaac tccgcgctga gccatgctcc gccggcgccg
     1861 atttccagga ttcggccccg gaattccaag ccgcacttct ccttcaggaa cggaatgtac
     1921 ttggggaagt cgcccttcac acagcgtaac cactcgtcct cccggccaaa ctcgagcgcg
     1981 tgcctcgtcc cggtttcccg caaagccagc atatgctcgc gctcgcagcg ggcaagatac
     2041 gccttgtttt caatgtattt tgactcggcc atgttcatca gaacgcgcac aggttatggg
     2101 gcaattctcc ctggcgcaac ggtgttttat gaagccaggc gcgagcgtca ttctttctgc
     2161 ttccgttgct cggcgaagaa agctcggagg agggcgcggc attctggctc acgcaccccc
     2221 ggggtaatct cacagcgatg attcaacgtc gggaactgta gcaagttcaa cgcgctgccg
     2281 gcggcgcccg ccttgggatc gctcgcgccg aacaccaccc gggccaggcg cacatggacg
     2341 atagcgccag cgcacatcgg gcacggttct ttggtgacgt aaagtgtgca atccgttagc
     2401 cgccagtcgc caacggcgcc ttcggcctga gtcaacgcca gcatctcggc atgcgccgtg
     2461 gcgtctttga gcagctccac ctggttgaac gagcgagcaa tgacgcgacc ctcgcgcacc
     2521 accaccgcgc cgacgggcac ctcctcggcc gcgtaagcct tagccgcctg acgcagcgcc
     2581 tcgcccatga aatagtggtc gctctgcaaa tcaatgatag gttcgtccga catcaacaat
     2641 gctcccctcc acccatgtaa ctcatgtaac tcatgcaatc cccttaaccg ccctctcgcc
     2701 tcgcaacgct ggattcttcc agtgtccatt cgttccgccc gtctggatgg cagtggcaat
     2761 gcgcggcgaa aaagccgcgg cgatgctgcc agaaccaggg ccaaatgcgc tcccgatccg
     2821 tttgcggtat cttcaatccc ggcaaggctt cgtggggaaa atactgaaag cgtccttcgc
     2881 gatgcacggg cggcaacgct gttaatttcc gtttcacctc gaacaggaac atcaaccagt
     2941 ggctctggcc ttgataaccg tgctcactcg ccaggccggt cagatgcagg tcggaaggcg
     3001 agaccaatat gccgagttcc tcgtgcgcct cgcggcaagc gcaagcgtag ggagactcgc
     3061 ccgtgtccgt gtggagcttc ccgccgcacg ggctccacaa gcccaggttg ggctcctgcg
     3121 cccgctccaa cagcaacacc tcatcccgct cgttgaagca atacagcagc gtcgccagct
     3181 tgtaaggcag cttcatggtt cgcggtgagc gaggttcctg acacaataca ggctcttcgc
     3241 cgtgcggata aacaatgccc catgcgccat cgcaatggat gaccgcaccg gggcctcgcc
//
LOCUS       DATCGS010000094         8960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_8295, whole genome shotgun sequence.
ACCESSION   DATCGS010000094 DATCGS010000000
VERSION     DATCGS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8295"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIL39_01840"
     CDS             <1..624
                     /locus_tag="VIL39_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480200.1"
                     /translation="QVTAGSQRASRVVRIINNEDKPLTLSPPESDNRAFAATIKTNPP
                     NGELRLVIKTVPPLLPGRVQGKITVKTSSTNAPVLTVTAFTFVPPIITVTPSHLSLPP
                     GPLAAATSLSVMIRNDSTNALALSDATINAKDVGVDLQAVKPGRLFRATLTFPQGFKV
                     PQGQQVEFTFKSSHPLCPLVKVPVTEAAHPTASPGPSSAATPPAAAQ"
     gene            complement(892..3411)
                     /locus_tag="VIL39_01845"
     CDS             complement(892..3411)
                     /locus_tag="VIL39_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEY5480201.1"
                     /translation="MLLALLNPLPVSAAALRPPAVPLVTFNPYLSIWSEADRLTDDVT
                     RHWTHREHPLVSLIRVDGQAYRLMGSEPKDVPAMPQAGLEVTPTRSIYSFDDGKVQVT
                     LTFMTPALPHDLEALALPLSYLTWAVRSADGRTHTVSIYNSTSSLLAVNQPDEMVNLG
                     QGTAGQLTFLRAGTEEQAVLGSSGDDHRINWGYAYVAAPAAHAKTAIGGSRQLAVGFA
                     ERGELPAHNDTRLPRPANNGEPAMAFNFDLGTVSAKPVTCQVIVAYDEVYAIKYFGKK
                     LRPYWRRKGATPEEMLQQAAHDYPRLAQRCAEFDAELVADAEKAGGRQYAQICALAYR
                     QCIAACGLAADANQQPLFFTKENTSNGDIATVDVFFPMDPQWILLSPTLAKATLVPIL
                     TYASSWHWKFPSAPHDLGTYPIARGTDDGGEGMPVEESGNMLILCDAVAQAEGKADFV
                     SPWWPKLTQWAQYLERYGLDPENQLCTDDFMGHLAHNANLSIKAILGLAAYGDLCRMR
                     GDAANAEKYQGLAKTDAEHWMKVAAEGDHYRLAFDKTNTWSQKYNLVWDRILGLNIFP
                     PSVARQEIAHYKKVLQKYGLPLDSRTHLTKTDWTLWSATLAEAPADFEALISPLCDYL
                     SQTTARSPFVDSYVTDDVRSDGMHARPVIGGVFVKMLADRALWQKWAGRDTAKAADWA
                     ALPEPPKVKVVLPAADQASATWHYTTAKPGDDWFKPGFDCALWRQGQSGFGTAGTPGA
                     RIGTTWNTGDIWLRREFDVSACNWHDLQAWLHHDEDAEVYLNGVLALHTTGFVSGYEA
                     WPLSATGKATLRPGQNHIAIHCHQTAGGQYIDLGLVDVLAE"
     gene            complement(3468..4328)
                     /locus_tag="VIL39_01850"
     CDS             complement(3468..4328)
                     /locus_tag="VIL39_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480202.1"
                     /translation="MKEFSTPTRRAGGRVARLASAFTLIELLVVIAIIAILAALLLPA
                     LAKAKAKGLKASCINNLRQLGLAQTMYVNDYAQYTGCLRVTGGYYYIWPSRLLYLMGN
                     DRKAFWCPAALRESAWDLTNNTTLKTVTGLDGQPDRYGITSTTRFSYGINDWGLDLNN
                     RPQLGLGGDIDGALYLGPVKDSMVVSPSQMIMVGDVPALKDAALISFNANLDPTDPTS
                     GHTQWPSNRHSYRTDLVFCDGHVESPKRNDVINPTASNLWRNRWNNDNQPHNNVTWTV
                     NPVWANQLDM"
     gene            4699..7140
                     /locus_tag="VIL39_01855"
     CDS             4699..7140
                     /locus_tag="VIL39_01855"
                     /inference="COORDINATES: protein motif:HMM:NF022752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEY5480203.1"
                     /translation="MNKFTRAWQVAIKAFVSPATASNRAVLVLCCLVAGCWAILAKAP
                     DAAGAPSEQLLTNGDFEKGTEGWDAIWAREAGAAKAVLDTVERHGGDQALRIEHTGRG
                     DWSLRHSLDLKVQPGEIYELTAWVRVRGAGSVTLGVVTRDATGKALDWAYGRRSTTET
                     KGWQVLRSRFVVPPETATIWPRLIGDGPATVWCDDFVLTRQGSLAALRGQGLPAAVTI
                     SNAAIGVTLQTTNATFAVRDHRTNRTWVQRADGGHLVVLEAKADGRAMDLKLLDPVSM
                     REMAARARLDGDTPELVVVLRAEGEMDVPLDWPAPFASAKGQLLILPVNEGMSYPTDD
                     ESLPAMHYYLYGGHGLCMPWYGTVEGDTGWMAIIETPDDAAVSIPRRDGLLGLVPEWE
                     PQKQRFGPERVIRYVFLDGGGYVAMAKRYREYAKQTGLFKTLSEKRKSVPAVDLLVGA
                     VNIWCWDRDAAAWCRELRACGIQRILWSNALPPDQIKALNDQDVLTSRYDIYQDAMNP
                     ENFPRLRWKHPDWTSEAWTNDDLMIGADGQWVRGWEVETKDGKMVPCGTLCDRQAVAY
                     AKQRIPADQATHPYRCRFIDTTTASPWRECYHPKHPMTRTESKRFKMDLLNYVSEGCG
                     LVCGSETGHDAAVPFVHYFEGMLSLGPYRVPDSGRDMIRAWDEVPERVAKFQTGHGYR
                     LPLWELVFHDCVVAQWYWGDYNNKLPKLWDRRDLWNALYGTPPMFMFNRQIWSANKDR
                     FVKSYQTAAVVARATGYSQMLSHEWLTPDHAVQRTRFANGVVVTANFGDAPWSPPGGN
                     RLASLESRVEGLKPQ"
     gene            7273..8634
                     /locus_tag="VIL39_01860"
     CDS             7273..8634
                     /locus_tag="VIL39_01860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5480204.1"
                     /translation="MKDKPILTRRSFIRTAATSSVALSWLSATHAPRVFAAELAKPAL
                     LGGTPVHQGGWARWPQWREAWEPELLKVWRSGHWFRGSAEHVTDFERGYAKLLGAKSC
                     LATASGTTALIVSLHVMDVDAGDEVIVSPYTFIASYNAILTSKALPVFADTDPATLTV
                     DPASIESCITERTRAIMPVHIFGMPCDMDPINAIARKHKLAVVEDACQAWLAEYKGRK
                     CGTLGDLGCFSFQESKHIPSGEGGAITSMSEQLIDKCDSFHNCGRACGTNKGHGSFTQ
                     GNNYRMTQAQAVILTQQFDKLVQETEIRRANADYLSANLGKIPGITPVRLPENSRPVW
                     HLYSFRYDAARFNGLSRDKFATAMAAEGVPCGGVYREQYYDGLLDEAIASRGFKRLWS
                     AERLKAYRDSFQQLKGNKQVCETTVSITQNMLLADRSGMDDILEAIRKVQAHSAALAK
                     AQG"
     gene            complement(8625..>8960)
                     /locus_tag="VIL39_01865"
     CDS             complement(8625..>8960)
                     /locus_tag="VIL39_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480205.1"
                     /translation="STLPIQRCPSADKDLKSGFQIFVTLGGLSRAFFVAMRLGEDLGL
                     CMANWQITQLQFILFAIASWVNRHQQAVIEYLQEEKRVFLEQLGGKPKRFIDAQQMPL
                     VAFPAYGQP"
BASE COUNT         1731 a         2783 c         2800 g         1646 t
ORIGIN      
        1 caggtcactg ccggctcgca acgcgcatcg agagtcgtgc gcatcatcaa caacgaggac
       61 aaaccgctga ccctctcccc cccagaaagc gacaaccgcg cctttgccgc caccatcaag
      121 accaatccac ccaacggaga gcttcgattg gtcatcaaga ccgtgcctcc gcttttgccc
      181 ggccgggtgc agggcaagat cacggtgaaa acctcctcga ccaacgcacc ggtcctgacg
      241 gtaactgctt tcaccttcgt gccgccgatt attacggtga ccccttccca tctctccttg
      301 ccaccgggcc ccctggcggc tgcgacctcg ctctcggtca tgatccggaa tgattcaact
      361 aatgccctag cgctgtccga tgccaccatc aacgcaaagg acgtgggtgt tgatctccag
      421 gcagtcaagc ccggccgttt attccgcgcc acgttgactt tcccgcaggg attcaaggtt
      481 ccgcagggcc agcaagtcga attcaccttc aagtccagtc atcccctctg tcctctcgtc
      541 aaagtgccgg tcacagaggc tgcccatcca accgcctcac ctgggccttc gtcggcggcc
      601 accccccccg ccgcagccca gtagccctta gtggttccgg acacaaatcc ggtaacgaat
      661 catgggccag cccgcttata acgtgcgatt ttacgcgtgg tatcggcttt tggtaagagg
      721 tttacgacca ccctgaagcc accctgaagc caccctcagg ccatctggta gccaaccggt
      781 aggcaccctg aagccatcct caggttggcg actgtcatgt ggcataaagt ggccacacag
      841 cgaaatcggc acgccgagac cagaaagccg aacttccacc gacctttggg ctcattcagc
      901 cagcacatcc acgagcccca gatctatgta ctgcccgccg gccgtctggt ggcagtggat
      961 cgcgatgtgg ttctggccgg ggcgcagggt ggccttgccg gtggcgctca ggggccatgc
     1021 ttcataaccg ctaacaaacc cggtcgtgtg cagggcaagg acgccattca gatagacctc
     1081 agcatcctcg tcgtgatgga gccaggcctg caggtcgtgc cagttgcacg cggagacatc
     1141 gaactcacgc cgcaaccaga tgtcgcccgt gttccaggtc gtgccgattc tcgcgccggg
     1201 ggtgccggcg gtgccgaagc cactttgccc ttgccgccac aacgcgcagt cgaagccggg
     1261 tttgaaccaa tcgtctcccg gcttggcggt cgtatagtgc caggttgcgg acgcctggtc
     1321 tgccgccggc aggacgacct tgaccttggg cggttcgggc agcgccgccc aatcggcggc
     1381 cttggccgtg tcgcgcccag cccatttctg ccagagggcg cggtcggcga gcatcttgac
     1441 aaagaccccg ccgatcaccg gtcgcgcgtg catgccatcg ctgcgaacat catcagtcac
     1501 gtaggaatcc acgaaaggcg agcgcgcggt ggtctggctg agatagtcgc agagcggcga
     1561 gatcagcgcc tcgaagtccg ccggggcctc agcgagcgtg gcgctccaca gtgtccaatc
     1621 ggtcttggtc agatgcgtgc gcgagtcgag cggcaagcca tacttctgca acaccttctt
     1681 gtagtgggcg atctcctgcc gcgcgacact cggaggaaag atattcagtc ccagaatccg
     1741 gtcccagacg aggttgtact tctgactcca ggtgttggtt ttgtcgaacg ccaggcgata
     1801 atggtcgcct tcggccgcga ctttcatcca gtgctcagcg tcggtcttcg ccagaccctg
     1861 gtatttctcc gcgttggccg cgtcgccacg catccggcac aggtcgccgt aggccgcgag
     1921 gccgaggata gctttgattg aaagattggc gttgtgggcg agatggccca tgaagtcgtc
     1981 cgtgcaaagc tggttctcgg ggtccagccc gtatcgctcc aggtattgcg cccactgggt
     2041 cagcttcggc caccagggcg aaacgaagtc cgccttgcct tccgcctggg caaccgcatc
     2101 gcagagaatg agcatgttgc cgctctcctc gaccggcatc ccttccccgc cgtcgtcggt
     2161 cccgcgcgcg atgggataag tgccgaggtc gtgtggcgca ctcggaaatt tccaatgcca
     2221 cgaggaagca taggtcagga tgggcacgag cgtggcctta gccagcgtgg ggctcaggag
     2281 aatccactgc gggtccatcg gaaagaagac atccacggtg gcgatgtcgc cattgctggt
     2341 gttttccttg gtaaagaaga gcggctgctg gttggcgtcg gcggcgaggc cgcaagcggc
     2401 gatacactgg cgataggcca aggcgcaaat ctgcgcgtat tgccgcccgc ccgccttctc
     2461 ggcatccgcc accagttcgg cgtcaaactc cgcgcagcgc tgggccagcc ggggataatc
     2521 gtgcgcggcc tgctgcaaca tttcctcagg agttgcgccc tttcgccgcc aatagggccg
     2581 caacttcttc ccgaagtact tgatcgcata aacctcgtcg taagcaacga tgacctggca
     2641 ggtgactggc ttggcgctca cggtgccgag gtcgaagttg aacgccatcg ccggctcacc
     2701 attgttggcg gggcgcggca gccgggtatc attatgggcg ggcaactcac cacgttcggc
     2761 aaatccgacg gccaactgcc ggctgccgcc gatcgctgtt ttcgcgtgtg cggccggcgc
     2821 ggcgacgtag gcatagcccc agttaatccg atggtcgtcg ccgctggagc ccagaaccgc
     2881 ttgctcctcc gttcccgccc gcaggaacgt aagctggccg gcggttccct gccccaggtt
     2941 taccatttcg tccggttggt tgaccgccaa caacgagctg gtgctgttgt atatcgaaac
     3001 cgtgtgcgtc cgtccgtccg ctgagcgcac cgcccaggtg agatagctca gtggcagcgc
     3061 cagcgcctcc agatcgtgcg gcagcgccgg tgtcatgaag gtgagggtga cctggacttt
     3121 gccgtcgtcg aagctgtaga tgctgcgcgt cggcgtcacc tcgaggccgg cctgcggcat
     3181 ggctggcaca tctttgggtt cgctgcccat caggcgatag gcctgaccgt ccacgcgaat
     3241 caaactaacc agcgggtgtt cgcggtgcgt ccaatggcgg gtaacatcgt cagtcaaccg
     3301 gtccgcctcg gaccagatgc tcaagtaagg gttgaaagtg accaatggca cggccggcgg
     3361 gcggagcgcg gcggcggaaa caggcagtgg attgagaagg gcgaggagca tgcacaaatg
     3421 tgcgactctg gaccaagtga gactgcagcg gtgcatcata ttctcgatta catatcaagc
     3481 tgattggccc agaccgggtt taccgtccaa gtcacgttat tgtgaggctg attgtcgttg
     3541 ttccaccgat tacgccagag gttgctcgcc gtcggattta tcacatcgtt tcgctttggc
     3601 gactcgacgt gaccgtcgca gaagaccagg tccgtccggt aactgtggcg gttggatggc
     3661 cattgagtat gaccggacgt aggatcggtt ggatccaggt tcgcattgaa agaaatcagc
     3721 gcggcgtctt tgagcgcggg cacgtccccg accatgatca tctggctggg gctgacgacc
     3781 atgctgtctt tcaccggacc tagatataac gcgccgtcga tgtcaccgcc caaaccaagt
     3841 tgaggacggt tgttcaggtc caatccccag tcattgatac cgtaggaaaa gcgggtcgtc
     3901 gaggtaatcc cgtatctgtc aggctggccg tcaagtcccg taaccgtttt aagggtcgtg
     3961 ttgttggtga ggtcccaggc actttcacgc agggcggcgg ggcaccagaa agccttgcga
     4021 tcattgccca tgaggtaaag aagccgcgag ggccagatat aataatagcc gccggtcact
     4081 ctcaggcagc cggtatattg ggcgtagtcg ttcacataca tggtttgcgc caggcccagt
     4141 tgccgcaagt tattgatgca ggaggccttt aggcccttcg ccttggcctt ggccagcgcc
     4201 ggcagcagca gcgccgccag gatggcgata atggcgatga ccaccaacag ctcgatcaga
     4261 gtgaaagcgg aggccagccg ggcaacccgc cctccggcgc gccgggttgg cgtcgagaat
     4321 tctttcatat caataccata cagtatctgt ctagctcttg ccacttgaga cgatcctaac
     4381 atgggggccg tagggcgtca tgtggcataa actggccaca cacgatagtc gggacatccg
     4441 ccaatgggcg acccgccacg gggcgccgtc acgccgttaa ggcacgctgg ggcaattccg
     4501 ccccccgtaa cgtcaataaa ccggtggcac gccaaaaacc agtaactgtt caggtagtgg
     4561 ggcgggcgtc tcgcctgtca ggagggcgtc tcgccctcgg ttttcgcaac gcgggcgaga
     4621 cgcccggtgc ggcaggcgag acgcccgccc cactacctga acagttacgt ccaatgaaac
     4681 agaatgattg accggtatat gaacaaattc actcgtgcat ggcaggttgc catcaaagct
     4741 tttgtgagcc ctgccaccgc ctcgaatcgc gcggtgctgg tgctgtgctg cctggtggca
     4801 ggctgttggg ctatcctggc gaaagcgccc gacgcggctg gcgcgcccag cgaacaactc
     4861 ctcactaacg gggatttcga gaagggaact gagggatggg acgcgatttg ggcgcgcgag
     4921 gccggcgccg ccaaggcggt cctcgacacc gtcgagcgcc acggcggcga ccaggccctg
     4981 cgcatcgagc ataccggccg gggagactgg agcctcagac attccctcga cctgaaagtt
     5041 cagcccggcg agatctacga gctcaccgcc tgggttcgcg tccggggcgc gggcagcgta
     5101 acgctcggcg tcgttacccg tgacgccact ggcaaggccc tcgactgggc ttatggcaga
     5161 cggtcaacga ctgagacgaa ggggtggcag gtcctgcgct cccgtttcgt cgttccgccg
     5221 gagaccgcaa cgatttggcc gcgcctcatc ggggacggcc cggccaccgt ctggtgcgac
     5281 gatttcgtcc tgacgcggca agggagtctc gcggcgttgc gcgggcaggg tctgccggcc
     5341 gcggtgacga tcagtaatgc ggccatcggc gtcaccctgc aaaccaccaa cgcgacgttt
     5401 gccgtgaggg atcaccggac gaaccggacg tgggttcagc gggcggacgg gggccatctg
     5461 gtcgtcctcg aagcaaaggc tgatgggcgc gcgatggatt tgaaactcct cgatccggtg
     5521 tccatgcgcg agatggcggc cagggcgcgg ctcgacgggg atactcccga gctggtcgtc
     5581 gtcctgcgcg ccgagggcga gatggatgtc ccgctcgact ggcctgcccc gttcgcctcc
     5641 gccaagggac agttgctcat tctgcccgtc aacgaaggga tgagttatcc gactgacgat
     5701 gagtcgctgc cggcgatgca ttactactta tatggcggcc acgggctttg catgccgtgg
     5761 tacgggacgg tggaaggtga caccggctgg atggcgatca tcgagacacc ggacgacgcg
     5821 gcggtcagca tccctcggcg cgatggcctg ctcggcctcg ttcccgagtg ggagccgcag
     5881 aagcagcgtt tcgggccgga gcgcgtcatc cgctatgtct ttttggacgg cggcggctat
     5941 gtcgctatgg ccaagcgcta tcgcgagtat gcgaagcaga cggggctgtt caagacgttg
     6001 agcgagaagc gcaagagcgt tcccgcggtg gacctcctgg tgggggcagt gaatatttgg
     6061 tgctgggacc gggatgccgc cgcctggtgc cgcgaacttc gggcctgcgg cattcagcgc
     6121 atcctctgga gcaacgccct gccaccggac cagatcaagg ctctgaacga tcaggacgtc
     6181 ctcactagcc gctatgacat ctatcaggac gccatgaacc cggagaactt cccacggctt
     6241 cggtggaagc atcccgactg gacgagcgag gcatggacga acgacgacct gatgatcggt
     6301 gccgacggcc aatgggtgcg cggctgggag gtcgagacga aggacgggaa gatggtcccg
     6361 tgcggcacgc tctgcgaccg gcaggcggtg gcctacgcga agcagcgcat cccggccgac
     6421 caggcgacgc atccctatcg ctgccggttt attgacacca ccaccgccag cccctggcgc
     6481 gagtgctatc acccgaagca ccccatgacg cgcaccgaga gcaagcgctt caagatggac
     6541 ctgctcaatt acgtcagcga aggctgtggc ctggtctgcg ggagtgagac gggccatgat
     6601 gcggctgtgc cgttcgtcca ctatttcgag gggatgctca gtctcggtcc ctaccgcgtt
     6661 cccgactccg ggcgagacat gatacgggcc tgggatgagg ttccggagcg cgtcgccaag
     6721 ttccagaccg gccacggcta ccggttgccg ctctgggagt tggtcttcca cgactgcgtg
     6781 gtggcccagt ggtactgggg cgactacaac aacaaactgc cgaagttatg ggatcggcgg
     6841 gatctctgga acgccctcta tggcacgccg ccgatgttca tgtttaatcg ccagatctgg
     6901 tcggcgaaca aggaccgatt cgtgaagagc taccagacgg cggcggtagt ggcgcgggcg
     6961 acgggctact cccagatgct ttcccacgag tggttgacgc ccgaccacgc cgtccagcga
     7021 acgcgctttg ccaacggggt ggtggtcacc gccaactttg gcgacgcgcc ctggtcaccg
     7081 cccggcggca accgcctcgc gtcgctggag tcccgcgtcg aagggctcaa gccgcaatga
     7141 aacctgtttt caagcaaact catagggtgt gcagatgggc gatggctaaa ccagcaggga
     7201 agtaaaaccg cacccacgac tgacggcgcc gatggtgcgc acctaaacaa accaagcaaa
     7261 tgtagcaaag ccatgaaaga caaaccgata ctgacacgac gcagtttcat ccgtaccgca
     7321 gcgacgagtt ctgttgccct gagctggctc agcgcgaccc acgcgccccg ggtgttcgcc
     7381 gctgagctgg ccaagccggc cctgctcggg ggcacgcccg tccaccaggg cgggtgggct
     7441 cggtggccgc agtggcgcga agcctgggag ccggagttgc tcaaggtctg gcggagtggt
     7501 cactggtttc gcggctccgc tgagcacgtg acggacttcg agcggggcta cgccaaactg
     7561 ctgggcgcca aaagctgcct ggcgaccgcc agcggcacca cggcgctgat cgtcagcctc
     7621 cacgtgatgg acgtggatgc cggcgacgag gtgatcgtct ccccctacac cttcatcgcc
     7681 agctacaacg ccatcctcac cagcaaggcc ctgccggtat tcgccgacac cgacccggca
     7741 acgctcaccg tggaccccgc ctcgatcgag agttgtatca cggagcggac gcgggccatc
     7801 atgccggtgc acatcttcgg catgccgtgc gacatggatc cgatcaacgc tattgcccgc
     7861 aagcacaaac tggcggtagt cgaggacgcc tgccaggcgt ggctcgcgga atacaagggc
     7921 cgcaagtgcg gcacgctcgg cgacctgggg tgcttcagtt tccaggaatc caagcacatt
     7981 ccttccggcg aaggtggcgc gatcacgagc atgagcgagc aactgattga caagtgtgac
     8041 tccttccaca actgcggccg cgcttgcgga acaaacaagg ggcacgggag tttcacccag
     8101 ggcaacaact accggatgac gcaagcgcaa gcggtcattc ttacgcaaca gttcgacaag
     8161 ctagtgcagg aaaccgagat ccgccgcgcc aacgccgact atctgagcgc caatctcggg
     8221 aagatccccg gcatcacacc cgtgcgcctg ccggagaaca gccgaccggt ctggcacctc
     8281 tactcgttcc gctacgacgc ggcccggttc aacggcttgt cgcgcgacaa gttcgccacg
     8341 gcgatggcgg ccgaaggtgt cccttgcggg ggcgtgtacc gcgagcagta ttacgacggc
     8401 ctgctcgacg aagcgatcgc ttcgcgcggt ttcaagcgac tctggagcgc cgagcggctc
     8461 aaggcttacc gcgacagctt ccagcagttg aaaggcaaca agcaggtctg cgagacgacc
     8521 gtctccatca cccagaacat gcttctggct gaccgcagcg gcatggacga catcctcgaa
     8581 gcgattcgca aggtgcaggc ccacagcgca gcgctggcaa aggctcaggg ctgaccgtag
     8641 gcgggaaatg ctacgagcgg catctgctgg gcgtcaatga agcgtttggg tttgccgccg
     8701 agttgttcca ggaagacacg cttctcttct tggagatatt caatcaccgc ttgctggtgc
     8761 cggttcaccc aactggcgat ggcaaacagg atgaattgca gttgagttat ttgccaattc
     8821 gccatgcaca ggcccaaatc ctcgcctaag cgcatcgcga cgaaaaaagc gcgggagagc
     8881 ccgcctaatg tcacaaagat ttgaaagcca gatttgaggt ctttgtcggc tgacgggcac
     8941 ctttgaatag gaagggtgga
//
LOCUS       DATCGS010000095         7104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_255214, whole genome shotgun sequence.
ACCESSION   DATCGS010000095 DATCGS010000000
VERSION     DATCGS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255214"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VIL39_01870"
     CDS             complement(<1..1090)
                     /locus_tag="VIL39_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480206.1"
                     /translation="MDFTMNNRWAAVAEPAWECNQAVFQDFFQRRGHRRNVLNCAIPS
                     ARAKVLTDFTMPEGWDADKSLIASVGFGAKLPPGLENWSYAPKIQAVKQLPQMKELPT
                     EHSTLMRSPWFQSPSQALGLGINARHLRYHYQLWNDLQPKTLRGVFTTLVYVTPQLGY
                     SLTPHIKLDRVFRYFGALSRFAGPAQLFPASYLYPDAKLQGMEKVPAAMFADILPNLG
                     SSFPLMDETPNPWNILVRLNGPDCYYGYPFSRVSNPVEDTTVSKVITGSMTLPNGKLV
                     QARNSESFCYATLDAKLKDALKLERYGFHDYAQQPNNFRTWRQSVCPPTDELAIPSKP
                     SNTKPSNTKKLNKTVKQSKNMTTTSLAEP"
     gene            complement(1100..2077)
                     /locus_tag="VIL39_01875"
     CDS             complement(1100..2077)
                     /locus_tag="VIL39_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5480207.1"
                     /translation="MKPIGISKLWKKHLGDWGVNVYNGCEHGCVFCYSPAQPGVKAQP
                     FWKGKTQADWGRFNSARDALVEALQHQLRTFTPARAVKTTWGQGRILVSFLCDAYQPL
                     EEKLLVTRQVLELLLQAGHRVRIQTRATLVRRDFDLLLKYRDQVQLGTSLPHLDDVLA
                     SVLEPNAPAPTARLEMLREAARVGIPVYVAIAPFLPFHKLDVLDEVVARVKPLRPTEI
                     FCEVLNPKGDCVGMVAQALATQYPAEAKIVAAYDDAAWARWTYEVLRHGVEHHSGMGF
                     MAWPDTGRAWSDHLTADKVTFLNQFLPPVEPNESVVKNCDISVGIGPLA"
     gene            complement(2174..2683)
                     /locus_tag="VIL39_01880"
     CDS             complement(2174..2683)
                     /locus_tag="VIL39_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480208.1"
                     /translation="MKNTIVPSLSPHSQTPPRLHHVTLNTGHVALHDLNGRHCVSARD
                     FLPLLEQPIGEIPGMPGFAFSVAEATQCCQFILSYQGSELITGGVAWGPGAGDSLWRW
                     LGDYYDCMAPWVPGWRTSCPRTPPALPWLGVVLNLNLGLIHHEQARQLAAVERDLALA
                     LIRRSLTRN"
     gene            complement(2730..3356)
                     /locus_tag="VIL39_01885"
     CDS             complement(2730..3356)
                     /locus_tag="VIL39_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480209.1"
                     /translation="MNEDTSFPEPPRTSNLNAREQLDKLFLDVASRAHGVMHRDGRLS
                     PTLFAVSPGGLSVYAPGPVNEAPEKNDFAAVARLICAAQAVTAVVLAVEAWVLEAKPG
                     QRLDLFTRPSQSPDRKEYLCLCGEAFGSIYQQKLLPILRDHRDRFSCLGTSKILPPEP
                     TAGRFANLLPQTHLNDDVRAFAALMLKSRGLVPGEALLESQLSAAARE"
     gene            complement(3459..4538)
                     /locus_tag="VIL39_01890"
     CDS             complement(3459..4538)
                     /locus_tag="VIL39_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480210.1"
                     /translation="MNKAPNPTGNRMDLAIPDSARSHKPPSQTSSPHDGAAKPVDPPD
                     RASSLAPSPESAAAQGANHSMRADPLRIQAKVTLAAERDAEASRAVLDLARSLEASTG
                     YPIQIGFEDLPPEADAYVEVAPLARRDCHVVHCTPFLSRPIYLHVLAHELTHIHLDHQ
                     ALTAGKAREFLPSARTRRYISGLYSTPARRFRRQGWSNAQIEEAVQADAGSLLMLLYN
                     CPVDLVVELWLSRHYPCLSAAQFLALYRLHQRDIRPGETLGQDSLRPRGLDIGSLALD
                     CVRALLHDEICAPATDFAAPYRDTEVFPLALRLWQHWKAVSPSLGPGDEFRLVDAFAD
                     ILGLRQAYESRPQAISAPRPLPNPA"
     gene            5488..6717
                     /locus_tag="VIL39_01895"
     CDS             5488..6717
                     /locus_tag="VIL39_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY5480211.1"
                     /translation="MNDFVRDLISFCEFGRRTQVLETIAATFGGEPLRVPTYVNEFWT
                     AKQRAAHSLHEISYRACFKPQLPRFFIQRLTQLGEVVYDPFMGRGTTLLETGLLGRVP
                     LGCDVNPLSIFLCRPRLNPPTLDQVTRQLAEIDFADADELPEDLLVFYHPNTLREVCA
                     LKKYLLGRQATSQLDAIDEWIWMVALNRLTGHSRGFFSVRTMPPNQAVSIQKQKRFNQ
                     ERCQVPQWKHVAAIIAKKTKALLSDCDDETRRMLAGVRARARWLTGRSSATPEIPAAS
                     VALVMTSPPFLDVVDYAGDNWLRCWFVGVDAQSVKFTAVKKLEDWQQAMVAVFRELHR
                     VLRPGGFVAFEVGEVRGGKLKLEHAVLPCGVAAGLRPKLVVIHEQKFAKTAHCWRVEN
                     NAKGTNTNRIVIFRKEQ"
BASE COUNT         1338 a         1965 c         2331 g         1470 t
ORIGIN      
        1 taggttcagc taagcttgta gttgtcatat ttttactttg tttgactgtt ttgtttaact
       61 tttttgtgtt tgacggtttt gtgtttgacg gttttgaagg tatggcgagt tcgtccgtgg
      121 gcgggcagac cgactgcctc caggtccgaa agttgttcgg ctgctgtgca taatcgtgaa
      181 aaccatagcg ctccagctta agggcgtcct tgagcttggc atccaacgtg gcgtagcaga
      241 agctctcgga gttgcgggcc tgcaccagct tgccgttggg aagcgtcatg ctccccgtga
      301 ttactttgct gacggtcgtg tcctccactg ggttcgagac ccgcgagaac gggtagccgt
      361 agtagcagtc ggggccgttg agccggacga ggatgttcca ggggttgggg gtctcgtcca
      421 tcagcgggaa gctggagccc aggttgggca ggatgtcggc gaacatggcc gctggcacct
      481 tctccatgcc ttggagctta gcatccgggt agaggtagga ggccggaaac agttgggcgg
      541 gcccggcaaa gcgggagagt gccccgaagt aacggaacac ccggtccagc ttgatgtgcg
      601 gcgtcaggct gtagccgagc tgcggggtga cgtaaaccag cgtggtgaac acgccgcgta
      661 gggtcttggg ctggaggtcg ttccagagct ggtagtggta gcgcaggtgg cgcgcgttga
      721 tgcccaggcc cagcgcctgc gatggcgact ggaaccaggg ggagcgcatc agggtgctgt
      781 gctccgtggg gagttccttc atctgcggga gctgcttgac cgcctggatc ttcggggcgt
      841 agctccagtt ctccaaaccc ggcgggagct tggcgccgaa gccgacgctg gcgatgaggc
      901 tcttgtccgc gtcccagccc tcgggcatgg tgaagtcggt cagcaccttg gcccgggcgg
      961 acgggatggc gcagttgagg acgttgcggc ggtggccgcg ccgctggaag aagtcctgga
     1021 atacagcttg gttgcactcc catgccggct ccgcgacggc cgcccagcga ttattcattg
     1081 taaagtccat ggggggtaat tatgcaagcg gaccaatccc aaccgaaatg tcgcaattct
     1141 tgacgacgct ctcgtttggc tccaccgggg ggagaaactg attcaggaac gtcaccttat
     1201 cggcggtcaa gtgatcggac cacgcgcgcc ccgtgtccgg ccaagccatg aagcccatgc
     1261 ccgagtggtg ctccacccca tgccgcaaca cttcgtaggt ccatcgagcc cacgcggcgt
     1321 catcgtaggc agccacgatt ttcgcctcgg ccggatattg cgtcgcgagc gcctgggcga
     1381 ccatcccgac acagtctccc ttggggttga ggacttcaca gaatatctcg gtcgggcgca
     1441 atggcttgac tcgggccacg acttcatcga gcacgtccaa cttgtgaaac ggcaggaagg
     1501 gcgcgatcgc cacgtaaacg gggatcccca ctcgcgccgc ttcgcgcagc atctccagcc
     1561 gggcggtggg cgcgggtgcg ttgggctcca gcacactggc caggacatcg tcgagatgag
     1621 gcaacgatgt ccctagctgc acctggtccc tgtatttgag caataaatca aaatcgcggc
     1681 gcaccagcgt ggcccgcgtt tgaatgcgga cgcgatggcc cgcttggagc aagagttcaa
     1741 ggacctgccg cgtcacgagc agtttctcct ccagtggctg ataggcgtcg cacaaaaacg
     1801 ataccaggat gcgaccctgg ccccaggtag tcttcacagc ccgcgccggt gtgaaggtgc
     1861 gcagttgatg ctgcagagct tctaccaaag catcgcgtgc gctattgaaa cggccccagt
     1921 ccgcctgcgt cttccccttc cagaaaggct gcgctttgac gcctggctga gcgggtgaat
     1981 agcaaaagac gcagccgtgt tcgcagccgt tgtaaacgtt gaccccccaa tcgcccaggt
     2041 gctttttcca taatttcgag atgccgatcg gtttcatgtt ttacctttct gctctttcga
     2101 gcggttgttt aatacggtga tgatggttgc gctcgcctct tcactgttgc catcggcttg
     2161 cgctgaaacc cctttaattg cgcgtcagcg aacgccggat caaggccagc gccaggtcgc
     2221 gctcgacggc ggccaattgc cgggcttgct catggtggat caggcctaaa ttcaggttca
     2281 gcaccacccc cagccagggc agggcgggcg gcgtgcgcgg gcaggaagtt cgccagccgg
     2341 gaacccaggg tgccatgcag tcatagtagt cacccagcca gcgccagagg ctgtcacctg
     2401 cgcccggccc ccaggccacg ccgccggtga tgagttccga cccctgatag gacaggataa
     2461 attggcagca ctgggtcgcc tcggccaccg agaaagcaaa accgggcatg ccgggaatct
     2521 cgccgatggg ctgttcgagc aatggcaaaa aatcgcgggc gctgacgcag tgcctgccgt
     2581 tcaaatcatg cagcgcgaca tggccggtgt tgagggtgac gtgatggagc cggggtggcg
     2641 tctgggaatg tggcgataga gaggggacga tggtgttctt cataatatgg tgttctgagt
     2701 tcgttggttt gctaacagcg agggagacat cattcgcgag cagccgcgga caactggctt
     2761 tccagcaggg cctctcccgg caccagcccg cggcttttga gcatcagggc ggcaaaggcc
     2821 cggacatcgt cgttcaggtg agtctgcggc agcagatttg cgaatcggcc ggcggttggt
     2881 tcggggggca gaattttgga cgtgcccagg caggagaatc ggtcgcgatg gtcccgcaaa
     2941 atgggcagca gcttctgctg gtagatgctg ccgaacgctt cgccgcaaag gcagaggtat
     3001 tctttgcggt caggtgactg tgaaggacgt gtaaagaggt ccaaccgctg gccgggtttg
     3061 gcttctagga cccacgcctc gacggcaagg acgacggcgg ttacggcctg ggcggcgcag
     3121 atgaggcgcg cgacggccgc gaaatcgttc ttctccggcg cctcattcac agggcccggg
     3181 gcataaacgg acaatcctcc tggcgacaca gcgaacagcg tcggactgag gcgaccgtcc
     3241 ctgtgcatga cgccatgtgc gcgagaggcg acgtcgagga agagcttgtc gagttgctcc
     3301 cgggcgttga gattggaggt ccgagggggt tctgggaagg aggtatcttc gttcatatcg
     3361 cgagttgttt cagaggttgg ttaggcttgg tttttaagcc gtctcggtgc ctcgccttgt
     3421 tgggcgtagc cccggcaatg ggcctgtgca ttccagtgtc aagcggggtt gggtaatggc
     3481 ctcggtgcag aaatggcttg aggccttgat tcatacgcct ggcgcaggcc aaggatatcc
     3541 gcaaaggcgt ccacgagccg gaattcgtcg cccggcccca gcgaagggga aaccgccttc
     3601 caatgctgcc acagccggag ggctagcggg aacacctcgg tatcgcgata cggcgcggca
     3661 aaatcggtcg ctggtgcgca gatctcgtcg tggagcaggg cgcggacgca gtccagcgcc
     3721 aggctgccga tgtcgagccc acggggcctt aagctgtcct ggcccagggt ctcgcccgga
     3781 cggatgtcgc gctggtgcaa acgatagagg gcgagaaact gggcggccga caaacaggga
     3841 taatggcggc tgagccagag ctccacgacc aagtcgactg ggcagttgta taggagcatg
     3901 aggagagagc cggcgtcggc ctggacggcc tcctcgattt gggcgttgct ccagccctgc
     3961 cgccggaagc ggcgcgcggg cgtcgagtag agcccggaga tgtaccgcct ggtccgggcg
     4021 ctggggagaa attcgcgtgc tttgcccgca gtgagggcct gatgatcgag atggatatga
     4081 gtgagttcgt gggcgagcac gtgcaggtag atcggtcggg aaaggaacgg cgtgcagtgg
     4141 acgacatggc agtcgcgccg ggcgaggggg gcgacctcga cgtaggcatc ggcttcggga
     4201 ggcaggtctt caaagccgat ctgaatgggg taaccggtgg aggcctcgag cgagcgagcg
     4261 agatccagga cggcgcggga agcctcggcg tcgcgctcgg ccgccagcgt tactttggcc
     4321 tggatgcgga gggggtccgc gcgcatcgaa tggtttgcgc cttgcgctgc ggccgactcc
     4381 ggggatgggg cgagcgatga cgcacggtcg ggcgggtcga ctggctttgc cgccccgtcg
     4441 tgaggcgagg aagtctggga gggtggcttg tgagatcggg cggaatcggg gatggcgaga
     4501 tccatgcggt tgccggtggg gttcggtgcc ttattcattt tcgtcgagtt gcgggccacg
     4561 gaccctatgc tgccgtcacc tgtctcgctt ggggttagcc cagataagac aggcgcgctc
     4621 gaggattgtt tagaggattt atcgtcgttc atcgtgcgga cttttctttt gagacttgtt
     4681 tgcccgtttt tcgtgttatt tcactgcctc gctttgttgg gcgtagcccc gggcaatggg
     4741 tataacggcg cagtcgagtt ctctggcgat tgcgcaggga acgaggtggg tgccgccggt
     4801 gttgatggac gccgggatct tgtcggtgcg atggacgcga atgacatccg gaccagcaga
     4861 tgcccgcagc ggtgatcgtg ttcctcgaac ccgactaagg aggcttcgcc aaggatgcgc
     4921 caggcgcttt ggaaggtccg cagggggggc accgtgattt ctctcgtacg ttgggtgttt
     4981 cgggaaacct tcgaacaaac cgggatccaa ttggaaatca cttcttctag cactcttgcg
     5041 acgttacaac gcttgtgtga aagagcatcc gaccaggcgg ttgaaccgtc gcacggctca
     5101 ggcggagttt gaagcccaga gaactagcgg ttccttggga gcagttccgc cgcaggtggc
     5161 aactctgaat cgactccgcg gaagttggcc cgcaggttct caagggtcat cactcggccc
     5221 cgtgtgctgg ttgcacctca acaaactcgt ctcaaaggga caaacatgag cgataacgat
     5281 aaccagcctc caaactccga aaagccgatc gtatccggcc ggctccgtgt cgccccgtgg
     5341 gaagccaaga gttcacctcc ccctcaactc acccctcaac tcagactttt ctgcgacccc
     5401 gatgcttttc agtttatttc tccctcctcg cattcggaac cgccgcgcgc tcaaacccgg
     5461 aacaaccgct ccgataattc gtcgtatatg aatgacttcg tccgggatct catttcgttt
     5521 tgtgagtttg gcagaagaac ccaggtgctc gaaaccatcg ccgcgacttt tggcggagag
     5581 ccgttgcgcg tccccaccta tgtaaacgag ttctggaccg cgaagcagcg agccgcccac
     5641 agcctgcatg aaatctccta ccgggcctgc ttcaaaccgc agttgccgcg gttcttcatc
     5701 cagcggctga cgcagctcgg ggaagttgtt tacgatccgt tcatggggcg cgggacgact
     5761 ctccttgaaa ctggattgct cggtcgggtt ccgctcggct gcgatgtcaa cccgttaagc
     5821 atttttctat gccgcccgcg tctcaaccct cccaccttgg atcaggtgac gcgccaactg
     5881 gcggagattg atttcgccga cgccgatgag ctgccggaag atttgttggt gttttatcat
     5941 cccaacacac tgcgggaagt ttgcgctttg aagaaatacc tcttggggcg tcaggcaacg
     6001 tcgcaactcg acgcgatcga tgaatggatc tggatggtcg ctctcaatcg gctgaccgga
     6061 cactcacgcg ggtttttctc ggttcgcacc atgcccccga accaagcggt ttcaatccag
     6121 aaacagaaga ggttcaatca agagcgctgc caggttccgc agtggaagca cgtggcggcg
     6181 attatcgcca agaagaccaa agcgttgctc tcggactgcg acgacgaaac gcgccgaatg
     6241 ttggcggggg taagggcgcg cgcccgatgg ctgaccggcc gctcgtcggc caccccggag
     6301 attccggccg ccagcgtcgc gttggtcatg acgtccccac cgttcctgga tgtggtcgat
     6361 tacgccgggg ataactggct gcgctgctgg tttgttgggg tggatgctca atctgtgaaa
     6421 ttcacggctg tgaagaaatt ggaggattgg cagcaggcaa tggtcgctgt gttccgggaa
     6481 ttgcatcggg tcttgcgacc cggcggtttt gttgcttttg aagtcggcga ggtccggggc
     6541 ggaaagctaa agttggaaca cgcagtgttg ccctgcggtg tcgcggcggg cttgcgtccg
     6601 aaattggtcg tgatccacga acagaagttt gccaagactg cccattgctg gagggtagag
     6661 aataacgcca aaggcacaaa tacaaaccgc attgtgattt ttcgcaagga gcagtgagtc
     6721 gaaaccgcta ccgtgtgcgc ctgccaagac agaggctgat cctggttcca acgacggcga
     6781 tgggtccggg gaaagcgtga attgcctggc cagaaagaag ctgcctcagg gcgctggggt
     6841 ttcgtttcgt ggcgcctgtt gtggcctcgg atgggaacat cacgaaaccg attccaactg
     6901 gagttcaatt tgtccattgc tctgctggct tgatcaatag cttgaaccaa ttctccatag
     6961 gttgaatctg gttcagatcg cctggatttc gacccgaatc cacgccggct gctcgcgagc
     7021 tggcgaaacc gcaaccaggt gagttggggt cggaactcgg ctggtcagat cgccgggctt
     7081 gagggcgagt gcatcggcga caac
//
LOCUS       DATCGS010000096         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_651588, whole genome shotgun sequence.
ACCESSION   DATCGS010000096 DATCGS010000000
VERSION     DATCGS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651588"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VIL39_01900"
     CDS             <1..874
                     /locus_tag="VIL39_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY5480212.1"
                     /translation="DPDRLLARFLKFGRATAVAAARRALAEAGVETAEITGLIVNTCT
                     GYLCPGLSSYIAEDLGLKPSIRFLDLMGMGCGAAIPNLEAAAGMLARTGEGPVLSLAV
                     EICTATIFPSHEPELVVSNSIFGDGAAAAVLDLAREGRPGGLAQMVDFATGLFPQYRE
                     DLRYRTEGGRLRNHLTKRVPVIGARTVTEVASRLLARHGLTRNDIAWWAVHPGGTVVL
                     AQVAKELELAQEALRFSYHVFENYGNMSSPSVLFVLREILDSGRPQPGQKGMLLSFGA
                     GFSAFAALLEFTSV"
     gene            1451..1639
                     /locus_tag="VIL39_01905"
     CDS             1451..1639
                     /locus_tag="VIL39_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480213.1"
                     /translation="MTPQLSDSHNRTYECISRHPAVRNPGWLDVQWMPRPLAEVALAA
                     KRRRGMRNLLNDTGRVPP"
     gene            1692..1857
                     /locus_tag="VIL39_01910"
                     /pseudo
     CDS             1692..1857
                     /locus_tag="VIL39_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003192767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
     gene            2146..2322
                     /locus_tag="VIL39_01915"
     CDS             2146..2322
                     /locus_tag="VIL39_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY5480214.1"
                     /translation="MGGKTDVAKGRIKEAAGALTGNDSLRAEGRADQAVGKTKQAVQK
                     TVDAVTKAVKKATR"
BASE COUNT          456 a          754 c          845 g          480 t
ORIGIN      
        1 cgatcccgac cggctgctgg cgcgatttct caagttcggt cgtgccaccg ccgtcgccgc
       61 cgcccgccgc gcgttggcgg aggcgggcgt ggaaaccgct gagatcaccg gtctgatcgt
      121 caacacctgc accggctacc tctgcccggg gctctcctcg tatatcgcgg aagacctggg
      181 gctcaagccg tcgattcgct tcctggactt gatggggatg gggtgcggcg cggcgattcc
      241 gaatctcgaa gccgccgccg gcatgctggc ccgaaccggg gaaggtccgg tcctcagcct
      301 ggccgtggaa atctgcaccg ccaccatttt ccccagtcat gagccggaac tggtggtgag
      361 taattccatt ttcggtgatg gtgccgccgc cgccgtgctc gacctggcgc gggagggccg
      421 tccgggtggt ctggcgcaaa tggtggactt tgcgacgggt ctttttcccc agtaccgcga
      481 ggatctgcgt taccggacgg aaggcggccg cctgcggaac cacctgacca agcgggtgcc
      541 ggtcatcggc gcgcgcaccg tgacggaagt ggcgtctcgg ctcctggctc ggcacggact
      601 tacgcggaat gacattgcct ggtgggcggt ccatccggga ggcacggtgg tgctggccca
      661 agtcgccaag gaactggaat tggcccaaga ggccctgcgc ttttcctacc acgtcttcga
      721 gaactacggc aacatgtcgt caccctccgt tctgtttgtc ttgcgggaga tcctggacag
      781 cggccggcca caacccggcc agaaaggaat gctcctctcg ttcggcgccg gcttctccgc
      841 cttcgcggcg ctcctggaat tcacgtccgt ttgaactccg atgagtgggt tcgcctcaac
      901 tggcccatct tgccgttgcc gacaccgtgc cccggatcgc gctatgtcgt cggccttatt
      961 tgtcagggta ttgagcgaga tggcacgcgg cgttttggcg atgattggtg acacggtcca
     1021 ttctgctcat ttcatccagg gtcgttccta gcgtcgcctg gctggtggcg ccggtacagc
     1081 gtgcggcccg cgcggcaacg gacgatacag cgcaagcaat gcccatcggc acgcggccct
     1141 cacagatcag cggtcgacgg ctgcgcgctg gggccaaggc taacggctac gatgccacga
     1201 acgcagcggc gtttccggat ctagccgctt tcggatgccc gccgcgctac aatgaggcta
     1261 aagaggagcg aatgcgaaag catgggctcc caatcggatg actagatgaa gggcaacccg
     1321 gccaggccgg ggggcccgtt gacgagtaag ccgacgtggt tcagcacctt cgggtgttca
     1381 gccgcgtcgg ctttttttgc cttcgccggc gagggaaggc cctacggccc ggaacgaatg
     1441 gaagttgaac atgacgccac agttaagcga ttcacacaac aggacttacg agtgcatctc
     1501 ccgccatccg gcagttcgca acccgggctg gctggacgtg caatggatgc cccgcccgct
     1561 ggccgaggtc gcccttgcgg cgaagcggcg gcgtgggatg aggaacttgt tgaacgatac
     1621 gggccgtgtt ccgccgtgaa gcccgaggtg ggttccggcc acgtgcgcgg caatactgga
     1681 aggagttggt tatgggactg attctactgc ttctgttggt cttgctgctc gtcggcggcc
     1741 tgccgacttt ggccccacac ccgtaactgg ggttatcgcc ccaccgggat cctgggattg
     1801 gtgctggtga tcctggtggt gctgctgctc ctaaacgtca ttcgcttcgg cttctgagcg
     1861 gccccgtggc ggttgtcgag ccggatcggc cgcctttctt tcggctgtcg ccaagacaat
     1921 acggcaggcc tgcctgatgg tgcgggatag cgaacaacgc attcgcggac gatgaaggcg
     1981 ggagtagact gatggacacg tccaaactga tgatggcgca gcgggtcgcg caggcagtca
     2041 gcgagtgcca gcaacaacgg acgacggccg cggcgaatgc gccttagagc gccgagttca
     2101 gtgttttcgg tgtttcggaa cagctcagaa gaaggagtcg ccatcatggg tggcaagaca
     2161 gacgtggcga aaggtcggat caaggaagct gccggggcac tgaccggcaa tgacagcctc
     2221 cgcgcggaag gaagggccga ccaggcggtc ggcaagacca agcaagccgt ccagaagacc
     2281 gtcgacgcgg tcacaaaagc cgtgaagaag gcgaccaggt aagccaccat cggcggcgcg
     2341 ggctgcccat tggcaatctg accagccagt ggtcgccaac gcgtatgggt tcggaagaga
     2401 gttgtgcctg ttctgaatgg cgtcgaacgt cccgtcgggc aagacgaaag aggagtcatt
     2461 atgaaaggct ttgtgaagga catcgagggt ctcgccatca agaatgccga gtttcgccgg
     2521 gtgctgtata cggca
//
LOCUS       DATCGS010000097         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_107911, whole genome shotgun sequence.
ACCESSION   DATCGS010000097 DATCGS010000000
VERSION     DATCGS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107911"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VIL39_01920"
     CDS             complement(<1..1163)
                     /locus_tag="VIL39_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480215.1"
                     /translation="MKKAATLLLALTLLLSATLALSAQSSPQETAANEAVYRQANRIA
                     LRQKLVDARAAQDRRAPDKAAKLYDDAWDLVGRIGSGVDAEREQTITGLAAVRLELAR
                     AAQQKGDYKEARTQVDDVLRVEPTNAAAIGFKSGNEKLLAEQRGTIPSEAVRSQVPGF
                     IEEQVKASTLVHDAKLLYEMNKLEEADVKLKLALQEDPHNSAALYYLNLVSEAKFARA
                     AKVHEVTMRQDVRAVEQAWASPLKRELLPVPNPYARSNNLIYTGLGRQTIMTKLDRIR
                     LDTVKYDGLPLSEVIINLSDESRKRDPEKRGINFIVNQNVDSGGPTATAAPMIGPDGQ
                     PLPAPPPEPVDMSAISIKINPPLNDMRLADVLEAIVAVADRPIKYSIVDYAVV"
     gene            complement(1197..2018)
                     /locus_tag="VIL39_01925"
     CDS             complement(1197..2018)
                     /locus_tag="VIL39_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480216.1"
                     /translation="MDFLKKHYEKVLLGVVLLGLAVAAAYLLFKIPSEKQKLEDMRNT
                     LIHPNVKPLTNLDLTVADRALKRMATPAAVDFSPPNKLFNPMPWQKAVDGRLIKADAT
                     NVGPNAVTITKMTPLYLKLTLDSVTPLETGTRYVIGIEKEAAPNTRERNKTQKYCSLG
                     TKNDTFTLRDVKTPPGNPTNITVILELNDTRQRVEVSKEQPFRRVDGYMVDLKYAPEG
                     KTWTSRRVDAPLPFGGEDYKIVAITEDEVVLSANSNQKKWTVKYSASLVPRVEPR"
     gene            complement(2021..3100)
                     /locus_tag="VIL39_01930"
     CDS             complement(2021..3100)
                     /locus_tag="VIL39_01930"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HEY5480217.1"
                     /translation="MDWIKRNLYFLIGSLVALALMGLAGWYFYSKWELNNKILGELEG
                     QYGELKRLHEQKPHPGSDKVDNIKAAKDQQQELRDYVRKTRPYFQLCSPIPQPESGKL
                     TSQEFSSALSRTVDQMQRDAAKVSVALPPKDSKGTTYSFSFAAQKESLAYAPGSLEPL
                     SVQLGEVKAICTVLFAAKINSLDNLRRERVSEDDNKGPQTDYLSEKSVTNELAVLTPY
                     EVSFRCFSSELASVLAGFASSPCGLMIVKTINVESAPAAPANEQTAAPVFPAAPIYNN
                     PAPPPPRRGAEGPVSAEAAFASRYGMGPGRGAPRPAPRPAPVYAQPAPSAAPSANKGG
                     LPLVLDEKQLKVTLMLNVVKLVPSK"
     gene            complement(3102..>3510)
                     /locus_tag="VIL39_01935"
     CDS             complement(3102..>3510)
                     /locus_tag="VIL39_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480218.1"
                     /translation="AMDAFRRRYGLGPEGRGGMPAPPPPAAEPALAGPEAPPAAGAAP
                     GTLPSRRKAKSGDTNEVATLTLTFRAVSLKEVSGQADSDKGIAYAVLQELQNSPLFDP
                     DPQETKTDSEVTYDESGTFTFRIIARLRRPLKL"
BASE COUNT          590 a         1049 c         1108 g          763 t
ORIGIN      
        1 aacacaaccg cataatccac gatggagtac ttgatgggcc ggtcggccac tgccacaatc
       61 gcctccagca catccgccag ccgcatatcg ttgagcggcg gattgatctt gatggaaatg
      121 gcactcatat ccactggctc gggcggggga gccgggaggg gttggccgtc cggacctatc
      181 atcggcgcgg cggtggcggt gggcccgccg ctgtccacgt tctggttgac gatgaaattg
      241 atgccgcgct tttccgggtc gcgtttcctg gactcgtcgc tcaagttgat aatgacttcg
      301 ctgagcggca gcccgtcata tttcacagtg tcgagccgga tgcggtcgag tttggtcatg
      361 atggtctggc ggcccaagcc ggtatagatc agattattgc tgcgggcgta agggttcgga
      421 acgggcagca attcgcgttt gagcgggctg gcccaggcct gctcgacggc gcggacgtct
      481 tgccgcatcg tcacctcatg cactttcgca gcccgggcga acttggcctc cgacacaagg
      541 ttcaggtaat agagggcggc tgagttgtgc gggtcttcct gcagggccag cttgagcttg
      601 acatcggctt cctccagttt gttcatttcg tagagcagct tcgcgtcatg caccagcgtg
      661 ctggctttga cctgctcctc gatgaacccc ggcacctggc tgcgcacggc ttcgctggga
      721 atggtgccgc gctgctcggc cagcagtttc tcgttgccgc tcttgaagcc aatggccgcc
      781 gcattcgtgg gctcgacccg caacacatca tcaacctggg tgcgggcttc cttgtaatcc
      841 cccttctgtt gcgccgcgcg ggccagttcc aaccgcaccg ccgccagacc ggtgatggtc
      901 tgctcgcgct cggcgtccac gcccgacccg attctcccca ccaggtccca ggcgtcatcg
      961 tagagtttgg cggccttgtc cggggcgcgt cggtcctgcg ccgcgcgggc gtccaccagt
     1021 ttctggcgta gcgcgatacg gttggcctgg cggtaaacgg cctcgttagc cgccgtttct
     1081 tgcggcgacg actgggccga caacgctaga gtagcgctga gcaaaagggt caacgcgagt
     1141 aacagagtag ctgctttctt catggtttcg gttgcggttg gcaaaaagac tagcgactag
     1201 cgcggttcaa cgcgcggcac taggctggca ctgtatttga cggtccattt cttctggttc
     1261 gaattggcgg acaatactac ttcgtcttcc gtaatagcaa cgattttgta atcctcgccg
     1321 ccaaagggga gcggagcgtc aacgcggcgg gaggtccagg tcttgccctc cggagcgtac
     1381 ttcaaatcca ccatgtagcc atcaacgcgc cggaaaggct gctctttgga tacctccacg
     1441 cgctgccgcg tgtcgttcaa ttccaggatc acagtgatgt tggtcgggtt gcccggcggg
     1501 gtcttgacgt cccgcagcgt gaatgtgtcg ttcttcgtgc cgagcgagca atacttctgc
     1561 gtcttgttcc gctcccgggt attaggcgcg gcttccttct cgatgccgat gacgtagcgc
     1621 gttcccgtct ccagcggcgt caccgaatcg agagtgagct tgagatagag gggcgtcatc
     1681 ttggtaatcg tcaccgcgtt cgggccgacg ttggtggcgt cggctttgat gagccgcccg
     1741 tccacggcct tctgccacgg catgggattg aaaagcttgt tcggggggga gaaatcaacc
     1801 gccgcggggg tcgccatccg tttgagcgcg cgatccgcca cggtcaggtc caggttggtc
     1861 aagggcttga cattaggatg gatgagcgtg ttgcgcatgt cctccagctt ctgtttctcg
     1921 ctggggatct tgaagagcag gtacgccgcc gccaccgcca ggcccagcag caccacgccg
     1981 agcaggacct tttcgtaatg tttcttgaga aaatccatct ttacttggaa gggaccagtt
     2041 tcaccacatt gagcatcagg gtgactttca attgtttctc gtccagcacc agcggcagtc
     2101 cgcctttgtt ggcggacggg gccgccgagg gcgccggctg cgcgtacacc ggcgccggcc
     2161 ggggggcggg cctgggcgcc ccccggccgg gccccatccc gtaacgcgag gcaaaagcag
     2221 cctccgccga cacgggcccc tcggcgcccc tgcgcggcgg cggcggggcc gggttattgt
     2281 agattggagc ggccgggaac accggcgccg cagtctgttc gttggccggc gcagccgggg
     2341 ccgactcgac gttgatggtc ttgacgatca tcagcccgca cggcgagctg gcaaatccgg
     2401 ccagcacgga ggccagctcc gaactgaagc agcggaaact cacttcgtat ggcgtcagca
     2461 ccgccaactc gttggtcacc gacttttccg acaggtaatc ggtttgcggt cccttgttgt
     2521 cgtcttccga cacgcgctcg cgccgcagat tgtccagcga gttaatcttc gccgcgaaca
     2581 gaaccgtgca gatggccttg acctcgccca attgcaccga aagcggctcg aggctgccgg
     2641 gggcataggc gagactctcc ttttgggctg cgaacgaaaa actataggtg gtgcctttgc
     2701 tgtccttggg cggcaaggca acgctgactt tggcggcgtc gcgctgcatc tggtcaacgg
     2761 ttcggctgag ggcggagctg aattcctggc tggtcaattt gcccgactcg ggctggggaa
     2821 tgggggagca caactggaag tatgggcggg ttttccggac gtagtcgcgc agttcctgct
     2881 gctgatcttt ggcggcttta atgttgtcaa ccttgtcgga accgggatga ggtttctgtt
     2941 cgtgcaagcg cttcagctcc ccgtattgtc cttcaagctc cccgaggatt ttgttgttga
     3001 gctcccattt cgagtagaag taccaaccgg ccaagcccat cagcgctaat gccaccaggc
     3061 tcccgatcag aaagtataaa ttgcgtttta tccagtccat actacagctt gagcggacgc
     3121 ctcagtctgg cgattatgcg aaaggtgaat gtgccgctct catcataggt aacctcgcta
     3181 tcggttttgg tctcctgcgg gtccgggtca aagagggggc tgttttgcag ctcctgcagc
     3241 acggcgtaag caatcccttt gtcggagtca gcctgcccgg aaacctcctt caagctcacc
     3301 gcgcggaagg taagggttag agtggccact tcattcgtat cgccgctttt ggcctttcta
     3361 cgggacggga gggtgccggg agccgcgccc gccgcaggcg gggcttccgg gccggcgagg
     3421 gctggttccg cggcaggtgg cgggggagca ggcatcccac ctcgtccctc cgggcccagg
     3481 ccataacgtc tgcgaaaggc atccatggcc
//
LOCUS       DATCGS010000098         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_206483, whole genome shotgun sequence.
ACCESSION   DATCGS010000098 DATCGS010000000
VERSION     DATCGS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206483"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VIL39_01940"
     CDS             complement(<1..506)
                     /locus_tag="VIL39_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480219.1"
                     /translation="MAISVAIVEDNPEVRRNLSRYIGGAPGFRCACACASAEEALRVI
                     PQSSPDVVLMDIQLPRMSGITCTASLKKVLPSVPVMMLTVYEDTDAIFNALKAGASGY
                     LLKRSDPAKVLEALTELHHGGAPMTGEIARKVIESFHRAKATAPAQDKLTAREEEILD
                     HLAKGFVT"
     gene            complement(519..>3291)
                     /locus_tag="VIL39_01945"
     CDS             complement(519..>3291)
                     /locus_tag="VIL39_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5480220.1"
                     /translation="FTPLALPESRGVRSAVIQVAREDSGALWLSTEDGKVGRLADGQY
                     SVISTNWKAADRTGFQVRADFRNRLWALSGSGLYQLAGEGLILALQGQPGEYVVHCAS
                     RSGGWWLSAGGQVRLWRDGQLIAQASRPGVAAESITCGIEDRSGHLWLGTAGNGLFHC
                     DTNGAPMQFTKRDGLSSDSVRTLFEDGEGNLWVGTEDGGLDRLRLPLFTVYGLSQGLS
                     SERITTVSEGREGEIWVGTDGYGLNRLQGETARLVTDETSAASWRVSAVLADRQGRVW
                     AALRSGGVFRWQGGPFTPLANLTPAGYPTRSLFEDSHGAVWIGQRITRTLSRVQGDTV
                     STIELPQSMPLADVRTMAEDAAGNMWFGTDGTGLLRWKDGQFTRFTRENGLSSDFVWA
                     LRPEPDGTLWIGTYGGGLTRLKDGRAVTCTTRHGLVDDVICHIADDGRGQYWFSSNQG
                     IFRVGKAELNQFADGRQTRIQCVAYGRSDGLPALEYEGGCQPAGCRTRDGRLWFPSIR
                     GLVVVDPADVSTNTAAPPVHIERIIVDGKTTEAGDWQHKDVARMPARTGSLRSSHRSG
                     QTGLEVGPGARRLEFHYTGLNFSAPETLRFRHKLEGVDAEWVETGGQREASYNQLAHG
                     SYTFRVQACNREGVWNQAGDMLSFTVLPHFWQTGWFAGLFLVTFGGTVGSSVRYALRR
                     RHQRQLKLVQQLHALERERTRIARDIHDDLGGSLTEIGYLGALAVRDSQSLAEAREQL
                     GRIMDRTRELARRLDETVWAVNPKNDSLSHLATYVCHFAQEFLESTGIRCRLDVAPDL
                     PEVTLTTEVRHSVFLVIKEALNNSVKHSAATELKFRLAVSDGVMTIEISDNGRGFALE
                     ARQEAGNGLRNMAARMEEIGGQFQLRSLPAQGATVCLHLPLLPHEGDQRSDKRRPIQM
                     GHSAGGRNA"
BASE COUNT          596 a         1118 c          963 g          614 t
ORIGIN      
        1 ttggtgacaa aaccctttgc caggtggtcc agaatctcct cctcacgggc ggtgagcttg
       61 tcttgcgcgg gtgccgtcgc cttggcgcgg tggaatgatt cgatcacttt gcgggcgatt
      121 tcgcccgtca tgggcgcccc gccatgatgc agctccgtca gcgcttccag cactttggcc
      181 gggtcgctgc gtttgagcag gtaaccgctg gccccggcct taagggcgtt gaatatcgcg
      241 tccgtgtctt catacaccgt cagcatcatc accggcaccg acggcaggac cttcttgagt
      301 gacgccgtac aggtgatgcc gctcatccgc ggcagttgga tgtccatcag caccacatcc
      361 ggcgacgatt gcgggatcac gcgcaacgcc tcctcggcgg aggcgcaggc gcaagcgcag
      421 cggaatcccg gtgcgccgcc gatatagcgg gagagattgc gccggacttc cgggttgtct
      481 tcgacgatgg ctacagaaat tgccatatag ttgacagtct aggcgtttcg tccgccggcg
      541 gaatgcccca tttggatagg gcggcgcttg tccgagcgct ggtccccctc atgcggcagc
      601 aacggcagat ggaggcacac cgtggcgcct tgcgccggca ggctgcgtaa ttggaactgt
      661 ccgccgatct cctccatgcg cgccgccatg ttccgcagac cgttgccagc ttcctgcctc
      721 gcctccagcg caaagccccg gccattgtcc gaaatctcga ttgtcatcac gccgtcgctg
      781 acggccaggc ggaacttcaa ttccgtcgct gcggagtgct tcaccgagtt gttcagcgct
      841 tctttgatga ccagaaaaac actgtggcgc acctcggtgg tcagcgtcac ctccggcaga
      901 tcgggcgcca catccaatcg gcagcggatg cctgtggatt caagaaattc ctgggcaaag
      961 tggcagacat aggtggcgag gtggctgagg gaatcgttct tggggttcac ggcccagacg
     1021 gtctcatcga gccttctcgc cagttcccgg gtgcggtcca tgatgcgccc gagctgctcg
     1081 cgcgcctcgg ccaacgattg ggagtcgcgc accgcgagcg cacccagata gccgatttcc
     1141 gtgaggctgc cgcccagatc gtcgtgaatg tcccgggcga tgcgcgttcg ctcacgctcg
     1201 agggcgtgga gctgctgcac gagcttaagc tggcgctggt gccttcgccg cagggcgtac
     1261 cttacgctcg atcccaccgt acctccgaac gtcaccagga acaatccggc gaaccaccct
     1321 gtctgccaaa aatgcggcag caccgtgaag ctgagcatgt ccccggcctg gttccacacg
     1381 ccttcccggt tgcaggcctg gacgcggaac gtgtaagagc cgtgcgccag ttgattgtag
     1441 ctggcctctc gctggccgcc ggtctccacc cattcggcgt ccactccttc cagcttatgg
     1501 cgaaagcgca gcgtttccgg agcgctgaag ttcaggccgg tgtaatgaaa ttccagacgc
     1561 cgcgcgcccg ggccaacttc cagaccggtt tgaccagaac ggtgggaaga ccgcagtgaa
     1621 cctgttcttg ccggcatccg cgccacatcc ttgtgctgcc agtcgccagc ctccgtggtc
     1681 ttgccatcca cgatgattct ttcgatgtgc accggcggcg cagcggtgtt tgtgctcacg
     1741 tccgcgggat ccaccacgac caggccccgg atgctgggaa accagagcct gccatcgcgg
     1801 gtgcggcaac ccgctggctg gcagccgccc tcatactcca gggccggcag gccgtcggac
     1861 ctgccatagg ccacgcactg gatacgggtc tgcctgccgt cggcgaattg gttcaactcc
     1921 gctttgccca ctcggaaaat gccttggttt gagctgaacc aatactgtcc ccgcccatca
     1981 tccgcgatgt gacaaatcac gtcgtccacc aggccatggc gggtggtgca ggtcaccgcg
     2041 cgtccgtctt tgagccgggt cagaccgccg ccataagtgc cgatccacag ggtgccatcc
     2101 ggctcggggc ggagcgccca gacgaagtcg ctgctcagtc cattctcccg cgtgaagcgt
     2161 gtgaactggc catccttcca tcgtaacaaa ccggtaccgt cggtgccgaa ccacatgttg
     2221 cccgccgcat cctcagccat cgttctcaca tccgccagcg gcatcgattg gggaagttca
     2281 atggtgctaa ccgtgtcgcc ctgaacccgt gacagcgtcc tcgtaatgcg ctggccgatc
     2341 cagaccgcgc catgggaatc ctcgaataag ctgcgcgtgg gataccccgc gggggtcaaa
     2401 ttagccaaag gcgtgaaggg tccaccctgc caacggaaca cccccccgga acgaagcgcc
     2461 gcccaaaccc gtccttgacg atcggccaac accgccgaca cgcgccagga tgcagcagac
     2521 gtttcgtcag tgaccagccg ggccgtttcc ccctggagac ggttcaaccc gtaaccatca
     2581 gttcctaccc agatttcacc ctcccgcccc tcgctcacag tcgttatccg ttccgaagaa
     2641 agaccctggc ttagtccgta aactgtgaaa aggggcaagc gcagccggtc caacccgccg
     2701 tcttccgtcc ccacccatag attgccttcg ccgtcctcga acagggttcg caccgaatcg
     2761 ctgctcagcc catcccgttt ggtgaactgc attggggcgc cgttggtatc gcagtgaaag
     2821 aggccattgc ccgctgtgcc aagccagaga tgcccgctgc ggtcctcaat gccgcacgtg
     2881 atgctctcgg cggccacgcc cgggcggctc gcctgggcaa tcaattggcc atcccgccac
     2941 aggcgcacct gaccgcccgc gctcagccac caacccccgg agcggctggc acagtgaacc
     3001 acatattcac caggttgacc ctgcagcgcc agaatcaaac cctcacccgc cagttggtaa
     3061 agcccggagc cgcttagagc ccaaagccgg ttccgaaagt ccgctcgcac ttgaaagcct
     3121 gtccggtccg ccgctttcca gttcgtcgag atgaccgaat actggccgtc ggccaagcgg
     3181 cccaccttcc cgtcttcggt tgagagccag agcgcgccgg aatcctcccg ggcgacctgg
     3241 ataacggcag agcggacgcc tcgcgactcg ggcaaggcaa gcggggtaaa g
//
LOCUS       DATCGS010000099         3335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_255733, whole genome shotgun sequence.
ACCESSION   DATCGS010000099 DATCGS010000000
VERSION     DATCGS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255733"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..551)
                     /locus_tag="VIL39_01950"
     CDS             complement(33..551)
                     /locus_tag="VIL39_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480221.1"
                     /translation="MTEALQLYSAATPPVSNRRRQLVPWRDPHTVPAEELTRHIAALE
                     AACVENPDSADLRTCLGMAYAMNLDAYKSMDVLEAAVRLEPENYFAQLKYAELLYRLR
                     ALPRAEEETLKALELAGNSRELALARAQLQEIRRLWRDGTQKPAWTKPLLQPTLLLMA
                     MCVILFLVVVLR"
     gene            complement(556..2178)
                     /locus_tag="VIL39_01955"
     CDS             complement(556..2178)
                     /locus_tag="VIL39_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5480222.1"
                     /translation="MKTRLRIALASMLLAAGAWAQSSAPAAEVRITGADLKAIEAPKA
                     ESRLRGDPDGSLTGTVADVAVSDPKKGLTLADVVNQVGQNKIAINFTWTLVAAFLVMF
                     MQAGFAIVETGLCRAKNANHTMMMNFMVYGVGMLAFWLIGFPLQMGGVGAIGNLGGTP
                     PLNSEFVVNLFGKPFGLFGQNGFLLMHSGAYDVSVMVVFIFQMVFMDTALTIVTGSAA
                     ERWKYSAFLISSFLMGAFTYPLFANWAWGGGWLANLGTNFGLGHGYVDFAGSGVVHAV
                     GGLSALALAIVIGPRIGKFTRSGKPNAIPGHDLVLVLTGCFILAFGWFGFNPGSTLGA
                     SGNGNLRIASVAVNTMLAGMAGSFGAMLYMWMRYGKPDASMSGNGLLAGLVAITAPCG
                     FVNPVSAVIIGLIAGVLVCLSVEFVERKMKVDDPVGAISVHGANGLWGVISVGLFADG
                     TSNYGGSWNGVNGSVTGLFYGDPSQLVAQLFGVSALLGVVFVSTFVLNLAIDAVVGQR
                     VSAAVELDGLDLPEMGALAYPEFELKARTSGA"
     gene            complement(2187..2369)
                     /locus_tag="VIL39_01960"
     CDS             complement(2187..2369)
                     /locus_tag="VIL39_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480223.1"
                     /translation="MVVAGLLVTVLGFGASLLSLGLPLSPGTRLAIVLAGIAVSLAGI
                     IGLLNPAYVKNAVWKK"
     gene            complement(2380..2559)
                     /locus_tag="VIL39_01965"
     CDS             complement(2380..2559)
                     /locus_tag="VIL39_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480224.1"
                     /translation="MGTNSTQAFGVTFFLAAFTLIAVGMARGGSLLLILPGLALLAIS
                     GAVLLRAKALDQEED"
     gene            complement(2590..>3335)
                     /locus_tag="VIL39_01970"
     CDS             complement(2590..>3335)
                     /locus_tag="VIL39_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5480225.1"
                     /translation="YVSYKPASLKGLQMDFGKYVTSAGAEVIETHSNWNYSRSLLFAW
                     AIPYYHMGLRTSMPVGQHFTAGFHLVNGWNNVEDNNSGKTIGLSGAVAAGPVTWSNNY
                     YAGPEKTNTNQGWRHLYDTTVLVNGSKASAYLNFDYGVDKLVTTGSNAWYGVAGAARF
                     ALTDHVAFTPRLEWFKDRDGFSTGTAQTLKEFTLTGEYKWTQGLLSRLEYRRDLSDQP
                     FFERGVTPGGSKNQTTLLAGFVAYFGPKR"
BASE COUNT          693 a         1078 c          964 g          600 t
ORIGIN      
        1 caccagagtc gccgtcgcga aaacgttcca cttcatcgca gcaccaccac caggaacaga
       61 atcacgcaca tagccatcag caggagagtc ggctgcagca agggcttcgt ccaagcgggc
      121 ttttgcgtgc catcccgcca cagccgccgg atctcctgca actgcgccct cgccaatgcc
      181 agctcccggc tgttgccggc aagctccaag gctttgagcg tctcttcctc cgcgcgcggc
      241 agcgcccgca gccggtagag aagctccgca tatttcaact gcgcgaagta gttttccggc
      301 tccaggcgca cagccgcttc gagtacatcc attgacttgt acgcatccag gttcatcgcg
      361 taggccatgc cgaggcaggt gcgcagatcc gcggaatccg gattctccac gcaggctgcc
      421 tcgagcgcgg cgatgtggcg tgtcaactcc tcggccggca ccgtgtgagg atcgcgccac
      481 gggacgagct gccgccgccg gttcgagacc ggcggcgtag cggcggaata cagttgtagt
      541 gcctcagtca tgggattagg ctcctgaggt gcgcgccttc agttcgaatt ccggataggc
      601 caaggcaccc atctcgggta gatcgaggcc atcgagttcg actgccgccg agactctctg
      661 gcccaccacg gcgtcgatcg cgaggttcaa cacgaaggta gatacaaata ccacacccaa
      721 cagcgccgac acgccgaaaa gctgcgccac aagctggctc ggatctccat aaaagaggcc
      781 ggtcaccgag ccgttgacgc cattccagga gccgccgtag ttgctggttc cgtcggcgaa
      841 gaggcccact gaaatcacgc cccacaaacc gttggcgccg tgcaccgaaa tggcgcccac
      901 gggatcgtcc actttcatct tgcgctcgac gaactcgacc gacagacata cgagcacgcc
      961 ggcgatcagg ccgatgatca cagcagacac aggattgaca aacccacagg gcgccgtgat
     1021 tgcgaccaag cccgccagga ggccgttgcc ggacatggag gcgtcaggct tgccgtagcg
     1081 catccacatg taaagcatgg ctccgaagct tcccgccatt ccggccagca tggtgttgac
     1141 cgctaccgag gcaatgcgca ggttgccgtt tcccgacgcg cccagcgtcg agccggggtt
     1201 gaaaccgaac cagccgaagg ccagaatgaa gcaaccggtg aggaccagaa ccaagtcgtg
     1261 gccgggtatc gcattcggct ttccgctacg ggtgaacttg ccgattcgag gcccgatgac
     1321 gatggcaagg gcaagcgccg acaggcctcc caccgcatgc accacgccag agcccgcaaa
     1381 atcgacgtag ccatgcccca gaccaaagtt cgtccccaag ttggcaagcc agccgccgcc
     1441 ccaggcccag ttggcgaaca gagggtaggt gaaggcgccc atcagaaacg agctaatcag
     1501 gaatgccgag tatttccagc gttcggccgc cgagccggtg acgatcgtca gcgcagtgtc
     1561 catgaagacc atttggaaaa tgaacacaac catcacggag acgtcgtagg cgcctgaatg
     1621 catcagaagg aacccgttct gcccgaacag gccaaatggt tttccgaaga ggttgacgac
     1681 gaactccgag ttcaagggcg gtgtgcctcc caagttgccg atcgccccta cgccgcccat
     1741 ttgaagcggg aagccgatca gccagaaggc caacatcccg acgccataga ccatgaagtt
     1801 catcatcatc gtgtggttgg cgttcttggc gcggcacagg cccgtctcaa caatggcaaa
     1861 ccccgcctgc atgaacatta cgagaaaggc tgccaccaac gtccaggtga aattgatggc
     1921 gatcttgttc tggcccacct gattgacgac gtccgcgagc gtaagcccct tcttcgggtc
     1981 gctgaccgca acgtcggcga cggtgcccgt gagcgatcca tccggatcgc ccctgaggcg
     2041 gctctcggct ttaggagcct cgatcgcctt caggtccgcg ccggtgatgc ggacctccgc
     2101 cgccggggcg gacgactgcg cccaggcccc cgcggccaag agcatgctgg cgagcgcaat
     2161 gcgtagtctc gttttcatat cgtctcctat ttcttccaaa cggcattctt cacatacgca
     2221 gggttcagca gccctatgat tccagccagg cttaccgcga tgccggccag cacgatcgcc
     2281 agccgcgtgc cgggcgagag cggtaacccc aggctgagca agctggctcc aaaccccaaa
     2341 actgttacga gcagacctgc gacgaccatt tcttcctcct cagtcttctt cttgatccaa
     2401 agccttggcg cgcagaagca ccgcgccgct gattgccagc agcgccagcc ccggcaagat
     2461 caacaacagg ctgccgcccc tcgccattcc caccgcaatc aaagtgaacg cggccagaaa
     2521 gaacgttacg ccgaacgctt gagtgctatt ggttcccatg gtgaattcct ttgtctattc
     2581 cgagtctcgt taccgcttcg gcccgaagta agccacgaaa cccgctaaca gcgtcgtctg
     2641 gttcttagat ccgcctgggg tcacgccccg ctcgaagaag ggctgatcgg acaaatcccg
     2701 gcggtattcg aggcggctga gcagcccttg cgtccacttg tactcgcccg tgagcgtgaa
     2761 ctccttcagg gtctgtgccg tgccggtcga aaagccgtcg cggtccttaa accactccaa
     2821 acggggcgta aaagcgacgt ggtctgtaag ggcaaaacga gcggcgccag cgacgccgta
     2881 ccaggcgttg ctgccggtgg tgacgagctt gtccacgccg taatcgaaat tgaggtaggc
     2941 gctggctttc gagccgttga ccaggacggt cgtgtcgtag agatgccgcc agccctggtt
     3001 tgtgttggtc ttctccggcc ccgcgtagta gttgttggac caggtcacgg gtccggcggc
     3061 cacggcgccg ctcagcccga tggtctttcc ggagttgttg tcttcgacgt tattccagcc
     3121 gttcacgaga tggaatccgg ccgtgaagtg ctggcccacg ggcatgctgg ttcgcaggcc
     3181 catgtggtag taaggaatgg cccacgcgaa caacaacgag cgcgaatagt tccagttact
     3241 gtgcgtctca atcacctcgg cgccagccga ggtcacgtac tttccgaagt ccatttgcag
     3301 ccccttcagg ctggccggct tgtaactcac gtagg
//
LOCUS       DATCGS010000100         3307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_553605, whole genome shotgun sequence.
ACCESSION   DATCGS010000100 DATCGS010000000
VERSION     DATCGS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553605"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VIL39_01975"
     CDS             complement(<1..256)
                     /locus_tag="VIL39_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480226.1"
                     /translation="MRPLFERLGMERSSAIVKLPEEIFKGSIFNCQDCAQCILHYNGM
                     TCPMNCPKQLRNGPCGGVRLNGKCEVKPEMDCVWVKGYERS"
     gene            complement(368..1252)
                     /gene="folD"
                     /locus_tag="VIL39_01980"
     CDS             complement(368..1252)
                     /gene="folD"
                     /locus_tag="VIL39_01980"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY5480227.1"
                     /translation="MTAKLIDGTAIGADVRREVTEQVAALKAQGGIVPNLSVVLVGDD
                     PGSQSYVSSKGKACTQAGMDSVTHRLLATASQAEVEHLVASLNADPHVNGILVQLPLP
                     KGLNEEAVLNGISLDKDVDGFHPINIGRLVMKGRVPLFVPCTPAGCIILLERSGVPIA
                     GATAVVLGRSNIVGIPVAALLNNRDATVTICHSRTPNIAEVVRRADILVAAIGKPEYV
                     RGDWIKPGAAVIDVGINRVEDPSAKRGYHLVGDVCFSEVVQVAGAITPVPGGVGPMTI
                     AMLLANTLHAFKLQHERA"
     gene            complement(1264..2166)
                     /locus_tag="VIL39_01985"
     CDS             complement(1264..2166)
                     /locus_tag="VIL39_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5480228.1"
                     /translation="MYDLIIIGGGPAGLTAAIYAIRKRMNCLLISPDLGGKANLRMVI
                     EGGTTYQVINGSDIVRRFRGELEYLEFARLLEKVTHLEKAGDHFALTTDSGRQFEAKS
                     VILSMGADPVRLNVLGADRYFMRGVAYSTVSYAPLYMDKQVAVVGSNGMALRGAAELA
                     QIASKVHLIAPDHGELDSNLGRRVLASKRVTLYEGWQPESVAGDSEVRQLVISSPSAE
                     QCTLDVDAVFVELGLVPRTGLVEGWVELDEQKRIVTSCGTETSVPGLFAAGDITHLPA
                     EQVLIAIGEGAKAALNAYEYLLNG"
     gene            complement(2228..>3307)
                     /locus_tag="VIL39_01990"
     CDS             complement(2228..>3307)
                     /locus_tag="VIL39_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY5480229.1"
                     /translation="IGPKAGLPVMEGKALLFKYLGGVDAVPLMLDTKDPDEIIHTVLM
                     LQPGFGGVNLEDISQPKCFRVLDTLREKAEIPIWHDDQQGTACVTVAGLLNALKIVGK
                     PVNEVRVVFIGSGAANVAISRLCFKAGVDPAKALMVDSKGILGRGREDIYQRRAEYVD
                     KWRLCEITNAEGRQGGIAEALVGADVCMACSRPGPGTIKPEWVSAMEHDAIVFACANP
                     VPEIWPWEAEAAGAAIVATGRSDFPNQVNNSLGFPGIFRGTLDVRAKTITDEMCIAAA
                     EALAGSAPDRGLDPQCILPVMTEWEVFAREAVAVGMKAQEQGIAGLALSADELYRHAA
                     DMIHRSRHITEAMMEQGFIEQPPAD"
BASE COUNT          605 a         1147 c          931 g          624 t
ORIGIN      
        1 gcgagcgctc gtagcctttg acccacacgc agtccatctc cggcttgacc tcgcacttgc
       61 cgttcagacg caccccgcca cacggaccgt tacgcaactg cttgggacag ttcatcgggc
      121 aggtcatgcc gttgtaatgc aggatgcact gggcacaatc ctggcaattg aagatcgagc
      181 ctttgaagat ttcctcgggc aacttcacga tggcggacga tcgctccatc cccaggcgtt
      241 cgaacagagg ccgcatacgc ggtagtattt tctcggcgag ttgccaggcc caaacgagag
      301 gtgcgggatg gtcctgtaga tatgtacgta ttgacatggg tttcgtcctt cgtctattta
      361 gtcgccctca agcgcgctcg tgctgcagct tgaacgcgtg caaggtgttc gccagcagca
      421 tggcgatggt cataggtccg acgccgccgg gaacaggagt gattgcgccg gcgacttgca
      481 cgacttcgct gaagcagaca tcaccgacca ggtggtagcc gcgcttggcg gatggatctt
      541 caacgcggtt gatgcccacg tcgatgaccg cggcgcctgg cttgatccaa tctccgcgca
      601 cgtattcggg cttgccgata gccgccacaa ggatgtcggc ccggcgtaca acctcggcta
      661 tgttcggcgt gcggctatgg cagatcgtga cggtcgcatc gcgattgttc agtagggccg
      721 cgacgggaat gccgacgatg ttgctgcgcc cgagcacgac tgccgtggct ccggcgatcg
      781 gcacgccact gcgctccagt aagatgatgc agccggcagg ggtgcaaggc acaaacagag
      841 gcacacgccc cttcatgacc agccggccga tattaatggg atggaagccg tccacatctt
      901 tatcgaggct gatcccgttc agcaccgctt cctcgttcaa tcctttggga agcggcaact
      961 gcaccaggat gccgttgacg tgcgggtcgg cgttcagcga cgcgacaaga tgctcgacct
     1021 cagcctggct ggcggtcgcg agcaggcgat gcgtgaccga atccataccg gcttgagtgc
     1081 aggctttgcc cttcgagctt acatagctct gggagcccgg gtcatcgccc accagcacga
     1141 ccgacaggtt gggcacgatg ccgccctgcg ctttgagcgc agccacttgc tctgtcacct
     1201 cgcggcgcac atcggcgcca attgcagtgc cgtcgatcag cttggcagtc atagcacgtc
     1261 acctcatccg ttcagcaaat actcgtaggc gttgagcgct gccttagcgc cttcgccgat
     1321 ggcaatcagc acctgctccg cagggaggtg agtgatgtcg ccggcggcga agaggccagg
     1381 gacgctggtt tcggtgccgc aactggtgac gatacgcttc tgctcgtcca gctccaccca
     1441 accctcgacc aacccggtac gcggaaccag gccgagctcg acaaaaacgg catcgacgtc
     1501 cagcgtacac tgctccgcag agggtgaact gatcacgagt tggcgcactt cgctatcgcc
     1561 ggccaccgat tcgggctgcc agccctcata gagcgtgaca cgcttggagg caagcacacg
     1621 gcgtcccaga ttggagtcca gttcgccatg atcgggggcg atcaggtgca ctttggacgc
     1681 gatctgcgcc agttccgcgg cgccgcgcag cgccatgccg ttagagccga ccacggccac
     1741 ctgcttatcc atatacagag gagcatagct cacggtcgaa taggccacgc cgcgcatgaa
     1801 gtacctgtcg gccccgagca cgttcaagcg gacggggtcg gcgcccatcg acaggatgac
     1861 ggacttggcc tcaaattggc ggccgctgtc agtggtgagg gcgaaatgat cgccggcctt
     1921 ttccagatgg gtgactttct ccaatagccg cgcgaattcc aggtattcca actcgccccg
     1981 aaagcggcgc acgatgtccg aaccgttgat cacctggtag gttgtgcctc cctcgatgac
     2041 catgcgcaga ttggccttgc cgcccagatc cgggctgatc agcaagcagt tcatgcgttt
     2101 gcggattgcg tagatcgcag cagtcaaccc agccgggccg ccaccgatga taatgagatc
     2161 gtacatggcc acgcctcact ttcttcgtga ttacgactga cgatgcactg gcaatgcttc
     2221 agttttctca atcggccggg ggttgctcga tgaacccctg ttccatcatc gcctcggtga
     2281 tatgccggga acggtggatc atgtcggcgg catggcggta caactcgtcg gccgataacg
     2341 ccagcccggc gatcccctgc tcctgcgcct tcatccccac cgccaccgcc tcccgggcga
     2401 acacttccca ttcggtcatc accggcagga tgcattgcgg gtccagaccc ctgtccggcg
     2461 ccgagcccgc cagcgcttcc gccgccgcga tgcacatctc gtccgtgatc gtcttggccc
     2521 gcacgtccaa cgtcccacgg aagatccccg ggaaacccag cgagttgttc acctggttcg
     2581 gaaagtcaga ccgccccgtt gccacgatcg ccgccccggc cgcctccgct tcccacggcc
     2641 agatctccgg caccgggttg gcgcaggcga acacgatcgc atcgtgctcc atcgcgctca
     2701 cccactccgg cttgatcgtc ccaggccccg gccggctgca ggccatgcac acatccgccc
     2761 ccaccagggc ttccgcaatc ccaccctgcc gcccctcggc gttcgtgatc tcgcacagcc
     2821 gccacttgtc cacgtactcc gcccgccgct ggtagatgtc ctcccgtccc cgccccagga
     2881 tccccttcga atccaccatc agcgccttcg ccgggtccac ccccgccttg aagcacagcc
     2941 ggctgatcgc cacgttcgcg gcccccgacc cgatgaacac cacccgcacc tcgttcacgg
     3001 gcttgcccac gatcttcaac gcattcagca gccccgccac cgtcacgcac gccgtcccct
     3061 gctggtcgtc gtgccagatc gggatctccg ccttctcccg caacgtgtcc agcacccgaa
     3121 aacacttcgg ctgcgagatg tcctccaggt tcacaccccc gaaccccggc tgcaacatca
     3181 gcaccgtgtg gatgatctca tccggatcct tcgtgtcgag catcagcggc accgcatcca
     3241 ccccacccag gtacttgaac agcagcgcct tgccctccat caccggtaac ccagccttcg
     3301 gccctat
//
LOCUS       DATCGS010000101         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_454403, whole genome shotgun sequence.
ACCESSION   DATCGS010000101 DATCGS010000000
VERSION     DATCGS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454403"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..671)
                     /gene="nth"
                     /locus_tag="VIL39_01995"
     CDS             complement(3..671)
                     /gene="nth"
                     /locus_tag="VIL39_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5480230.1"
                     /translation="MACETKAATSARMQKIIAGLKKAYPDAHCELDHASPLELLVATI
                     LSAQCTDKRVNLVTANLFKQYRSAAGYAQAPLPELEQAIKSTGFFRNKAKSIKTGCQA
                     LVEKHGGQVPRTMEELTQLGGVGRKTANVVLGNAFGINCGVVVDTHVARLAQRLGLTK
                     ETAPEKIEQELMKLVSQEQWTLFSHWLIWHGRRRCYARNPDCPGCEIKALCPRRGVPE
                     AKAA"
     gene            complement(967..>2165)
                     /locus_tag="VIL39_02000"
     CDS             complement(967..>2165)
                     /locus_tag="VIL39_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480231.1"
                     /translation="VRQILASAAKDTLTKLVAEDKSLEPQFTAIAAVEKLARYNRDLY
                     KLLNNFVSFRDFYGRKDKAIFQAGTLYLDQRSCDLCLTVEDAGRHALLAGLAGTYLAY
                     CDCVRKATGEKLQIVAAFTGGDSDNLMVGRNGIFYDRKGRDWDATITKIIENPISIPQ
                     AFWAPYKKVVRMIEEQVAKRAAAADTAATQKFEQTAATVVQADQPQPAPPPQQPKTKM
                     DTGTLAAIGLVLATLLGALGGIFGAIAKLPAWQVPLAILGILLAISLPSMLLAWLKLR
                     KRNLSPILDANGWAVNAKAKINVPFGGSLTGVAALPPGSQRDLVDPFAEKKRPWALYI
                     TLAVIVALAIGWYFGNLDRALPGRAKSTSVLGTNAPAYMPPLGLTITNLAPAAAAPAK
                     PAEAAK"
BASE COUNT          371 a          663 c          704 g          427 t
ORIGIN      
        1 agtcaggcag cctttgcttc gggaacgccg cggcgcgggc agagcgcctt gatttcacaa
       61 ccgggacaat cgggattccg cgcgtagcag cggcggcggc catgccagat cagccagtga
      121 ctgaacagcg tccactgttc ctgggacacc agcttcatca actcctgttc gatcttctcg
      181 ggcgcggttt ccttcgtcag accgagccgc tgcgccaagc gggcgacatg cgtatccacc
      241 accacaccgc agttaatgcc aaacgcgttg cccagcacga cattggcggt cttgcggccc
      301 acgccgccga gttgcgtcag ttcctccatc gtccgcggca cctggccgcc gtgcttttcg
      361 acgagggcct ggcagccggt cttgatgctc ttggccttgt tgcggaagaa gccggttgat
      421 ttgatggcct gctccagttc aggcagcgga gcctgggcgt agccggcggc ggagcgatac
      481 tgcttaaaca ggttcgccgt gaccaggttc acgcgcttgt cggtgcactg cgccgacagg
      541 atcgtcgcca ccagcagttc aagcgggctg gcatggtcca gttcgcagtg cgcgtccggg
      601 tacgccttct tcaggccggc gatgattttc tgcattcggg cggatgttgc tgctttggtt
      661 tcgcaagcca taatcggact gaggttaagc gcgccgagcc tcggcagcaa gtccgccgga
      721 tcagttgtcg gatcagtcag gcacccgagc agttccttca cgtgcaaccg gctgctccag
      781 tgcccttcta tggagtgcgc cggcagagcg cagcggcgac ggcgctgttc cttgaggtgg
      841 attctgagac cgttcgaccg cagccatagc gccgtggccg ctccgctctg ccggcgcact
      901 ccaaagatgg cggtaaacga gcggcgactc gttgctccgt gaagggggtt gcgttggggg
      961 tcgcgattac ttcgctgctt cagccggttt cgccggggcg gcggcggcgg gcgccaagtt
     1021 ggtgatggtc agtcccagcg gcggcatata agcgggggcg tttgtgccca ggacggacgt
     1081 gctcttggcg cgaccgggca atgctcgatc cagattgccg aagtaccaac cgatggccag
     1141 cgcaacgatg acagcaagcg tgatatagag agcccagggg cgcttctttt cggcaaacgg
     1201 atcaaccagg tcccgctggg agccgggcgg cagcgcggcc acgcccgtga gtgaaccgcc
     1261 aaacggcaca ttgatcttgg ccttggcatt caccgcccag ccgttggcat cgagaatcgg
     1321 gctgaggttc cttttccgca gcttgagcca ggccagcaac attgacggta gggagatcgc
     1381 gagcaggatg ccaaggatag ccagcggcac ctgccaggct ggcaacttcg cgatcgcgcc
     1441 aaagatcccg cccagcgcgc ccagcagggt cgccaatacc aggccaatgg ccgccagcgt
     1501 gcccgtgtcc atcttggtct tgggctgttg gggtggcggc gctggctggg gctggtccgc
     1561 ctggacgacg gtggcggccg tttgttcaaa cttctgagtg gcggcggtat cggcggccgc
     1621 ggcgcgcttg gcgacttgct cttcgatcat gcgaacgacc ttcttgtaag gcgcccagaa
     1681 ggcctgcggg atgctgatcg ggttctcaat aatcttggtg atcgtggcat cccagtcgcg
     1741 ccctttgcgg tcatagaaga tcccattgcg gccaaccatg aggttgtcgg aatcgccgcc
     1801 cgtgaaggcg gccacgattt gcagcttctc gccggtggct ttgcgcacac aatcgcagta
     1861 ggccagatat gtgcccgcca gcccggccag gagcgcgtgc cgcccggcat cctcgacggt
     1921 caggcacagg tcgcagcttc gctgatccag gtagagcgtg ccggcttgaa agatcgcttt
     1981 gtccttacgc ccgtagaaat cccggaagct aacgaagttg ttcagcagct tatacagatc
     2041 acggttgtag cgggcgagct tttccaccgc ggcgatagcc gtgaactgcg gctccaagct
     2101 cttgtcctcg gcaaccagct tggtaagagt atccttagcc gcgctggcga gaatctggcg
     2161 gacgc
//
LOCUS       DATCGS010000102         4379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_602944, whole genome shotgun sequence.
ACCESSION   DATCGS010000102 DATCGS010000000
VERSION     DATCGS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602944"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VIL39_02005"
     CDS             complement(<1..480)
                     /locus_tag="VIL39_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5480232.1"
                     /translation="MLRRDRQIRMQIHQVMDACLFALGFWLAYKVRAHQDLIDLFGLR
                     EWTVPFDTYVRLYLILIPAAPLILERLGFYGRSPFCARSLTAWILLKGCFFTTLALVL
                     AMFLFHMPPVARGVVVWFGFISFGLVFVKEEALRRFASTKMAQAQYRRRVILVGASDE
                     "
     gene            complement(944..1033)
                     /locus_tag="VIL39_02010"
     tRNA            complement(944..1033)
                     /locus_tag="VIL39_02010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Ser,seq:cga)
     gene            complement(1052..1126)
                     /locus_tag="VIL39_02015"
     tRNA            complement(1052..1126)
                     /locus_tag="VIL39_02015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1092..1094),aa:Val,seq:cac)
     gene            1531..2853
                     /locus_tag="VIL39_02020"
     CDS             1531..2853
                     /locus_tag="VIL39_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5480233.1"
                     /translation="MIYHEKTPTLEEGRADQPEPYSLPRPAIAREVNNQPARRKAPAD
                     SHSCALLDKARDFKTADQLRKIGLYPYFRTISSAQDTEVIIEGQKVLMLGSNSYLGLT
                     NHPKIKAAAQAAVEKYGTGCAGSRFLNGTLDIHLELEEVLAKLVNKEAALLYSTGFQV
                     NLGVISALVGKGEYVLGDKSNHASIVEGCLVSQGKFLRFAHKDMAALESRLQKLEPEA
                     GKLIVADGVFSMEGDIIPLPELCRIAGQYGAAVMVDDAHSIGVLGKDGAGTASHFGLT
                     DQVHLIMGTFSKSLASLGGFIASDAETIDYLKHHSRPLIFSASMSPANAAAVLAAVEI
                     MKQEPERLAQLWRNTHRMKQGLRDLGFNLGDSETPILPVYCRDLMLAFKICKRLQEEG
                     LFVNPVVSPAVAPGQELIRISLMATHTDRQVDFALDKLGKVGKELGLI"
     gene            2854..3834
                     /locus_tag="VIL39_02025"
     CDS             2854..3834
                     /locus_tag="VIL39_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5480234.1"
                     /translation="MKILLTGASGFVGSHILDSLRARGLATVLLLRPTSNKQFIASHL
                     PDVELRPGSIGDLESLQQAMAGITHVIHCAGATKAGRVAGFYEANQAGTRNVISAVNG
                     QRDQVQRIVHISSLAAAGPARREQPAREQDPPRPVSEYGKSKLAGELEVRNHCRIEHV
                     ILRPPAVYGPRDAEFLRLFKAVKNHVLPKPSGAQALSLVFVKDLAEAVVTCLRHPAAA
                     GKTYFVASREVVTARSMAEEIAAQMNVWTVPLPLPTTFLWPICLVQEVMSRLTGKPSV
                     LSLQKFAELRAPGWVCDPTRLERETGCVCATTLKTGVAEALAWYRKQHWL"
     gene            3831..>4379
                     /locus_tag="VIL39_02030"
     CDS             3831..>4379
                     /locus_tag="VIL39_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480235.1"
                     /translation="MKNYTFVDYATQAYMALVGLLILLFHNSTVPDWPRLLGAHVVGL
                     VLVHWLIQAHARRKPAPPLDFLRHFYPVLLYTAFFVETGSLNRMFFREYLDPMVVQWE
                     QGLFGCQPSVLFMQKLPWLAVSELFYASYCSYYFMVAGVGIALFLRNRQQFFHYVSVI
                     SFVFYACYLIYIFLPIIGSRAFF"
BASE COUNT          896 a         1371 c         1272 g          840 t
ORIGIN      
        1 ttcgtcgctc gcgccgacga ggatcacccg ccgtcggtac tgggcctggg ccattttggt
       61 gctggcgaac cgccgcagcg cctcttcctt gacgaatacc aaaccgaagc tgatgaagcc
      121 aaaccacacc accactccgc gggccaccgg cggcatgtgg aacaggaaca tcgccaggac
      181 caacgcgagc gtcgtgaaga agcaaccctt gagcaagatc cacgcggtca ggctcctggc
      241 acaaaagggc gaacgcccgt aaaaccccag ccgctccagg atcagcggcg cggcggggat
      301 caaaatcaga tataggcgga cgtaggtgtc gaatggcacg gtccactccc tcaggccaaa
      361 gagatcaatg aggtcctggt gggcccgcac cttgtaggcc agccaaaaac cgagggcaaa
      421 caagcacgca tccatcacct gatggatttg catccgaatc tggcgatcgc ggcgcaacat
      481 ctctcgttat cttgttaacc tgtgacggat ttcaacaggg ttagaccgga ctcgtcgagc
      541 ataatcttcg gcgctccaca caggcgcatc tgaccttttg cacaccctat aggttggctc
      601 gaacggctga tgttgtcccg ctcttcggcc ccttcggtga aatcagggcc cgcccaaacc
      661 tgggaagggc atggggcgga atgaaccagg ccgaagctca ggcgtctaat gagagacgct
      721 ggagccagct tggcttggcg tcgccgaggc ggtcgaggga ccgtaacgag gggctgattt
      781 cccttgtacc ccatcgtaac cccttcgtga gcccttcgtg accccttcgt gaccccatcg
      841 taaccccatc ggagcatcac gggagcatca cgggagcatc aggcgggcat caccctgccc
      901 ccctccgaga gggcgcatgt caggaggcgc ctctttcgag agttggcgga agggagagga
      961 ttcgaacctc cggtaggctt gcgcctacgt ctgatttcga gtcaggtgcc tttaaccact
     1021 cagccaccct tccgtcgctc gaccgataag atggtgcgcg ctgcagggct tgaacctgcg
     1081 accccttccg tgtgagggaa gtgctcttcc actgagctaa gcgcgcgacc agtgccgaac
     1141 agcctccttc aaaggcctgc ttttggcggt ctgctgcccg atttcgagcg cctcaacttc
     1201 gtcaggccgc cgcagtttag caagccgaaa agaaccactc gcctcgaaaa acccggaata
     1261 atcttctccc gggcaccctc caaccgggcg ggcttgaagc cggcggctgg cagcccgggg
     1321 gcattttgca tcaaattaat acttgccagc cgggcgagaa agcgtaaaag ggaggccgga
     1381 aaaaggtcga aaagggttgc tgaacggttg tctggctttc gagactatga aaagacatgg
     1441 tgagtttgta acaagcggct ggcgatggat tacgagaacg aagcggcgag tcagcaaagc
     1501 tgacttcgca tcgcaatgcc ttgcctgttt atgatttacc acgaaaagac ccccaccctg
     1561 gaagaaggcc gagccgacca gcccgagcca tactcgttac cacgtcctgc catcgcccgg
     1621 gaggtaaaca accaaccagc ccgccggaaa gcgccggccg actcgcactc ttgcgcgttg
     1681 ctggacaagg cccgggattt caagaccgcc gaccaactgc ggaagatcgg gctgtacccg
     1741 tatttccgca ccatttcttc cgcacaggac accgaggtca tcattgaagg ccagaaggtc
     1801 ctgatgctgg gctccaacag ctacctgggc ctcaccaacc atccgaagat taaagccgcg
     1861 gcgcaggcgg cggtggagaa atatggcacc ggctgcgccg gctcgcgctt cctgaatggc
     1921 acgctcgaca tccacctgga gctggaagaa gtcctggcca aactggtcaa caaggaggcc
     1981 gcgctgctct acagcaccgg gttccaggtc aacctcggcg tcatcagcgc cctggtcggc
     2041 aagggcgaat acgtgttggg cgacaaaagc aaccacgcca gcattgtcga gggctgcctg
     2101 gtctcgcagg ggaagttcct gcgcttcgcg cacaaggaca tggccgctct cgaaagccgt
     2161 ctccagaaac tcgaacccga agcggggaaa ctcattgtcg cggacggcgt cttcagcatg
     2221 gagggcgaca tcattccgtt gccggaactc tgccgcattg ccggccaata cggcgcggcc
     2281 gtcatggtgg atgacgccca ctcgatcggc gtgctgggca aggacggcgc cggcacggcc
     2341 agccattttg ggctgaccga tcaggtccac ctgatcatgg gcacgttcag caaatccctc
     2401 gccagtctgg gagggttcat tgcctctgat gcggagacca ttgattacct gaagcaccat
     2461 tcccggcccc tcattttcag cgcgagcatg agcccagcca acgccgctgc cgtgctggcc
     2521 gcagttgaga ttatgaaaca ggagccggaa cgcctggctc agctctggcg caacacccac
     2581 cgaatgaagc aaggtctccg cgatctgggc ttcaacctcg gcgactcgga aacccctatc
     2641 ctgccagtgt attgccggga tctgatgctc gccttcaaga tttgcaagcg gctgcaggaa
     2701 gaaggtctct ttgtcaaccc cgtggtctca cctgcggtgg cgcccggaca ggagctgatc
     2761 cgcatcagcc tgatggcaac gcacacagac cggcaggttg attttgcctt ggataagctg
     2821 ggcaaggtgg gcaaagagct cggcctgatt taattgaaga tcttgctcac aggagcgagc
     2881 gggtttgtcg gcagccacat tctcgatagc ctgcgcgcac gaggccttgc caccgttctc
     2941 ctgctccggc ccaccagcaa caaacagttt atcgcctccc atttgcccga cgtcgaactg
     3001 cgcccgggct cgattggcga cctggaaagc ctacagcagg cgatggcggg catcacgcac
     3061 gtcatccatt gcgccggcgc cactaaggcg ggccgggtcg ccgggtttta cgaggctaac
     3121 caggccggga cgcgcaatgt tatcagcgcc gtcaacggac aaagggacca ggtgcagcgg
     3181 atcgtgcata tttccagcct ggccgctgcg ggacctgccc gccgtgaaca acccgcccgc
     3241 gagcaggacc cgccccggcc agtctctgaa tacggcaaga gcaaactggc cggcgagctc
     3301 gaagtgagaa accactgccg catcgagcat gtcattctgc gccccccggc ggtttatggt
     3361 ccgcgcgacg cggaatttct gcggctgttc aaggccgtga agaatcatgt gctgccaaag
     3421 ccaagtggag cacaggcgct tagtctggtc tttgtgaaag acctggcaga agccgtcgta
     3481 acctgtctcc gccacccagc cgcagccggc aagacctatt ttgttgcctc ccgggaagtc
     3541 gtgactgcac ggagcatggc agaagagatc gccgcccaga tgaacgtctg gacagtgccc
     3601 ctgccgctgc ccacgacctt cttgtggccg atatgcctgg tccaggaagt catgtcacgc
     3661 ctgacgggca agcccagtgt cctgagcctt cagaaattcg ccgagttgcg cgcgccgggc
     3721 tgggtgtgcg acccaacccg gctcgagcgg gaaactggtt gcgtttgcgc caccacgctc
     3781 aagacaggag ttgccgaggc cctggcctgg tatcgaaagc agcattggct gtgaagaact
     3841 acacctttgt tgattacgca acccaggcct acatggcgct ggtggggctg ctgattcttc
     3901 tctttcacaa cagcaccgtg ccggactggc ctcggctgct gggcgctcat gtggttgggt
     3961 tggtgctggt ccattggctg attcaggctc atgcgcggag gaaaccggcc ccgccgctgg
     4021 atttcctccg ccacttctat ccggtgctgc tttacacggc gttcttcgtc gagaccggct
     4081 cgctgaaccg catgttcttc agggagtacc tcgatccgat ggtggtccag tgggagcagg
     4141 ggctatttgg ctgccaaccc agcgtcttgt tcatgcagaa gctgccgtgg ctggccgtca
     4201 gcgagctctt ttacgcctcc tactgttcct attacttcat ggtcgccggg gtgggcatcg
     4261 ccctgttcct gcgcaaccga caacaattct tccactatgt ctccgtcatc tccttcgtct
     4321 tctacgcctg ctacctcatc tacatcttcc tgcccatcat cggctccaga gccttcttt
//
LOCUS       DATCGS010000103         5248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_454578, whole genome shotgun sequence.
ACCESSION   DATCGS010000103 DATCGS010000000
VERSION     DATCGS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454578"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..2090
                     /locus_tag="VIL39_02035"
     CDS             45..2090
                     /locus_tag="VIL39_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480236.1"
                     /translation="MKANQHSPLYTRANGIFLILILSGLLGTASGALAGEAPYYRDLF
                     PDTWVGHDALGRNMPPYSVVGPVKKDHRRVVGIFYITWHSDSAHNGKNPYGGDVSRIL
                     ATDPKARLDAKHPLWSEGAYHWGEPESGYFLSKDEYVIRKDMSMLTDAGVDVLIMDVT
                     NAVRYWDEWEAIFAVIEKMKAEGNQVPQFCFWAFNGPVITVVQDLYDRVYKVGKYKDL
                     WFIWDGKPLLLYNGTPTSDANGSGVQNPNPHYDAAAKTDTKHPHFGDQDYAEQFYKDY
                     TKEVKSFFTRRTMWWGYWEWAGKRYVGTEDNWSFGLDLGDKRVAKMTPDELLSKHNGQ
                     KEEAAVTPAQHPSSLVGKSWTREAGEPPLNERDLPEPTYVPWLGRKVEHPEGYGIYFQ
                     QRWDEAIQGDPQFLYLNDWSEWTAGKYSPGGSATTKFMCRDSTYFFVDQYNAEFNRCL
                     QPMKDGYTDNYYMQMAENIRRYKGVRPIPEVIGLHRIKIDGSFADWAGVEVEYRDTVG
                     DTFHRDYDGYGGLHYTNNTGRNDIITSKVAVDRDAVYFYVETKAALTPHTGTNWMLLL
                     IDSDQDPNTGWYGYDYLINLRVIDDKTTTIQRYAPNAPGGPWVEVARLKYRYAGKALE
                     MAVPRKLLGLKGDAFTFDFHWCDNPTDLKDPISLCTSGDSAPNRRFNYRCIWKKKSK"
     gene            complement(2576..3679)
                     /locus_tag="VIL39_02040"
     CDS             complement(2576..3679)
                     /locus_tag="VIL39_02040"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEY5480237.1"
                     /translation="MFAEPLSANGTTLVYFGTYTVAKSKGIYVSRFDSATGRLTAPEL
                     AVATPSPSFLAIHPSRRFLYAAGETTTLGAKRTGAVSAFSLDAKTGQLTLLNQQSSGG
                     GGPCHVAVDQSGKCLLVANYGSGSIEALPIQADGALAEPGTVIQHQGSSVNPARQAGP
                     HAHFITTDPANRFALACDLGLDQVLVYRFDPAKAALVANDPPFASVKPGSGPRHLAFH
                     PSGQFAFLINEMGSTLTVFAYDAKQGALKEVQTISTLPQEFTGKSSGAEVQVHPSGKF
                     VYGSNRGHDSITVFGFDPKRGQLTCLQHQSTQGKTPRHFAFDPTGKWLLAENQESDSV
                     VVFRVDAKTGSLNPTGQTISVGAPVCAVFVPAK"
     gene            3881..5026
                     /locus_tag="VIL39_02045"
     CDS             3881..5026
                     /locus_tag="VIL39_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5480238.1"
                     /translation="MSTDIQIVPLSRNPRDVMRFLKVSYRIYRDDPNWVAPLLMDLKK
                     VFTDANPLFEHAVMQLWVATRAGQDVGRIAGIIDRNHNHVAKAPAAFFGFFESVDDPA
                     VSRRLFETVSGWTRQAGLPRLLGPMNPTTNDECGLLVEGFDSPPVIMMTYNPRYYVTL
                     VEAAGFRKSKDLLAFHMDLAKIPMDRLARIAAKIKQRNPNLALRPVRRKTLAQDLAKV
                     KEVYNAAWEDNWGFVPMTDAEVDFMAARLKPLLMEGLIWLVEAGTEPVGFLLALPDYN
                     IALKPLRGRLLTPKVLGFIPYLLGWKCPPRTRVITLGMKREYRGKGLESAMLIEGLRV
                     GIKAGVTESEASWILEDNVMMCRMLEAIGGRPYKTYRLYERQIGPKA"
BASE COUNT         1109 a         1556 c         1637 g          946 t
ORIGIN      
        1 tccgcatttt accctaacca acacgatcaa cataagagct caacatgaaa gctaaccaac
       61 actcacctct ctatacgcgg gccaacggca tattcttgat cctgatcctg tcaggccttc
      121 tgggcacggc gagcggcgcg ctcgcgggag aagcgcctta ttaccgcgac ctctttcccg
      181 atacgtgggt gggacacgat gctttgggcc gcaacatgcc gccctactcc gtcgtgggac
      241 ccgtcaagaa agaccaccgc cgcgtagtgg gcatcttcta catcacgtgg cacagcgatt
      301 cggcgcacaa tgggaagaac ccttatggcg gggacgtgag ccgcatactc gccaccgatc
      361 caaaggcccg gctcgatgcc aaacatccgc tctggtcgga gggcgcgtac cactggggtg
      421 agccggaatc gggctacttc ctgagtaaag acgaatatgt catccgcaaa gacatgtcca
      481 tgctcaccga tgcgggagtg gatgtgctca taatggacgt caccaatgcc gtccgttact
      541 gggacgaatg ggaagccatc tttgcggtga tcgagaagat gaaagcggaa ggcaaccagg
      601 tgccgcagtt ctgcttctgg gcgttcaacg ggccggtgat caccgtggtg caagacctgt
      661 acgaccgagt ctataaggtc ggcaaatata aagacctctg gttcatttgg gatggcaagc
      721 cgctgctcct ctacaacggc acgcccacct cggacgccaa cggctcgggc gtgcagaatc
      781 cgaatccgca ctacgatgcc gccgccaaga ccgacaccaa acacccgcac tttggcgacc
      841 aggattacgc cgagcagttc tacaaggatt acaccaaaga ggtaaagagc ttcttcacgc
      901 gacgcaccat gtggtgggga tactgggagt gggcggggaa gcgctacgtc ggcacagaag
      961 acaactggag cttcggcttg gacctgggcg ataaacgggt cgcgaagatg accccggatg
     1021 agctgctctc caagcacaat gggcagaagg aagaagcggc ggtcaccccc gcgcagcatc
     1081 cctcgagcct ggtaggcaag tcctggaccc gagaagcggg cgaacctccc ttgaacgagc
     1141 gcgacctgcc ggagccgact tatgtgccgt ggctgggacg gaaggtcgag catcccgagg
     1201 gctacggcat ctacttccag caacgctggg acgaggcgat ccagggcgac ccgcagttcc
     1261 tctatctcaa tgactggagc gagtggaccg cgggcaagta ctcgccaggg ggcagtgcca
     1321 ccacgaaatt catgtgccgg gacagcacgt acttcttcgt ggatcagtat aacgccgagt
     1381 tcaaccgctg cctccaaccc atgaaggacg gatataccga caactactac atgcagatgg
     1441 ccgagaatat ccgccgctac aagggcgttc gcccgatacc ggaagtgatc ggcctgcacc
     1501 ggataaagat agacggaagt tttgcggatt gggcgggcgt cgaggtcgag tatcgcgaca
     1561 cggtcggcga taccttccac cgcgactacg acggctatgg cggcctgcat tatacgaata
     1621 acacgggccg caacgacatc atcaccagca aggtcgccgt tgaccgtgac gcagtgtatt
     1681 tctacgtcga gacgaaggct gcgttgaccc cgcataccgg caccaactgg atgctgctat
     1741 tgattgactc cgaccaggat cccaacacgg gttggtatgg ctatgattac ctcatcaacc
     1801 tgcgcgtaat agacgacaag acgaccacga tccagcgtta tgctcccaac gcccccggcg
     1861 gtccgtgggt cgaggtggcg cggctgaaat atcgttatgc gggcaaggcg ttggagatgg
     1921 ctgtcccgcg caagctgtta ggcttgaagg gagacgcttt tacctttgat ttccattggt
     1981 gtgacaatcc taccgacctg aaagatccca tctccctctg caccagcggg gatagtgcgc
     2041 caaaccggcg attcaactat cgttgtatct ggaagaagaa aagtaaatga gggctagatg
     2101 actgaaccgg gaccagtcag gtggggcagg gggccgacgg gttcagagtg cttagccgta
     2161 gcgtcaacaa actcacgcgt tctcgcggta gtagaatctc aataatccag cgagtcgccc
     2221 acgctttcga atggttccag aggggcagcc gcgcggcggc tacgggagga atacaccgca
     2281 aaactgaacg gcaagcagcc agtggccgcg tggagcgggc aaaaagcccc agaactagag
     2341 gaacaataga ggaataatag aggaccaatc gagagattca cgggggctgc acgggggtta
     2401 caccaacgga acaggccaac ctcccgccct gcgcaccaac gggggtgggg ctcatcgcgg
     2461 gagcgtcacc ggacgcacat gggagcctcg ccggggatac cattcatact cccaggggcg
     2521 cctacaacct ccgccgggcc tcgtgatata acccgggctt acggtcggcc gacggtcact
     2581 tggccggcac aaacacggca cagacgggcg caccaactga gatcgtctgc ccagtcgggt
     2641 tcaggctgcc ggtctttgcg tccacacgaa agacgaccac gctgtctgac tcctggttct
     2701 cggccagcag ccatttgccc gtcgggtcga aggcaaagtg ccggggcgtc ttgccttggg
     2761 ttgattggtg ctgcaggcaa gtcagttggc cgcgttttgg atcgaagcca aaaaccgtga
     2821 tactgtcgtg gccgcgattt gagccataga caaacttccc cgaaggatgc acctgtactt
     2881 cggcgccgga actcttgccg gtgaattcct gcggcagagt cgaaatggtc tgcacttcct
     2941 tcagtgcccc ctgcttggcg tcgtaggcaa acacagtgag cgttgagccc atttcgttga
     3001 tgaggaaggc gaactggccg gacggatgaa aggccaggtg gcgcgggccg gagccgggct
     3061 tgacggaggc gaagggcggg tcgttggcca cgagcgcggc tttggccggg tcaaaccggt
     3121 aaacgagcac ctggtccagg ccgaggtcgc acgccagggc gaaccggttg gcggggtcgg
     3181 ttgtgatgaa atgggcgtgg ggaccggcct gccgcgccgg gttcacgctc gagccctggt
     3241 gttgaatgac ggtgcccggc tccgccaacg cgccgtccgc ctgaatcggc aaggcctcga
     3301 tgctgccgct gccgtagttg gcgaccagga ggcatttccc cgattggtcc accgccacgt
     3361 ggcaagggcc cccaccgcca gaggattgct ggttgagcaa cgtgagctgg cctgtctttg
     3421 cgtccaggct gaaggcgctg acagcgccgg tgcgcttggc gcccagtgtg gtcgtttccc
     3481 cggctgcgta gaggaaccgc cggctgggat ggattgccag aaagctgggg ctgggcgttg
     3541 ccaccgccaa ctcgggcgcg gtgagccggc cggtggcgga gtcaaagcgg gaaacgtaaa
     3601 tccccttgct cttggcgacc gtgtaagtgc cgaaatagac cagcgtagtg ccgttggcgg
     3661 aaaggggttc tgcgaacacg gcgggatggt aaataagccc gcacccaagc acaggccaaa
     3721 taaggtggaa gcgtgacagc aatttcatcg ggctagagaa catcttccgc ggccaatttc
     3781 aacaaggaaa cgtccctgcc gggttgaccg ccgggccggc ttgcttcacc ctttgggccg
     3841 gctggtgacg gcttcccggc tccgccgcgt tatttcgagc atgagcactg acatccagat
     3901 tgttccgttg tcccgcaacc cacgggatgt gatgcgcttc ctgaaggtca gctaccgcat
     3961 ttatcgggat gacccgaatt gggtcgcccc gctgctgatg gacctgaaga aggtcttcac
     4021 cgacgccaac ccgctgttcg agcatgccgt aatgcagctc tgggtcgcca cccgggcggg
     4081 ccaggacgtt ggccgcatcg ccggcatcat tgaccgcaat cataaccacg tggccaaagc
     4141 cccggccgcc ttcttcggct tcttcgagtc ggtggacgac cccgccgtca gccgtcggct
     4201 attcgagacc gtctccggct ggacccgtca ggcaggcttg ccacgactgc tgggaccgat
     4261 gaaccccacg accaacgacg agtgcggttt gctggtcgag ggcttcgatt ccccgccggt
     4321 catcatgatg acctacaacc cgcgctacta cgtaacgctg gtcgaggcgg cgggctttcg
     4381 caaaagcaag gatctgctgg cgttccacat ggacctggcg aagatcccga tggaccgcct
     4441 ggcccgcatc gccgccaaga tcaaacagcg caatcccaac ctcgccctcc gtcccgtccg
     4501 gcgcaaaacg ttggcacagg acctcgccaa ggtgaaggag gtctataacg cggcctggga
     4561 ggacaactgg ggctttgtgc cgatgaccga tgccgaggtg gacttcatgg ccgcgcgcct
     4621 gaagccgttg ctgatggaag gcctgatctg gctggtcgag gcggggaccg aacccgtcgg
     4681 gtttctgctg gcgctgccgg attacaatat cgcgctcaaa cccttgcggg gacggttgct
     4741 gaccccgaag gtgctggggt tcattcccta tctgctcggc tggaagtgcc cgccccggac
     4801 ccgggtcatc accctgggca tgaagcgaga gtatcgcggc aaaggcttgg agtccgcgat
     4861 gttgatcgag ggtctcaggg tcggcatcaa agctggcgtc acggagtccg aagcctcctg
     4921 gatactggag gacaacgtca tgatgtgccg catgctcgag gccatcggcg gccgcccgta
     4981 caagacgtat cgtctctacg agcggcagat tgggccgaag gcgtgagcaa aggggagggg
     5041 atggcctaaa tccgaaggcc gaggaccgaa ggccgaaaga aacccgaaac tcgaaatccg
     5101 aaaagatctg gaacgacgaa ccactgctct gcgagcgcgg cagtggattc ggccttcggc
     5161 cttcggactt ctttcggcct tcggccttcg gacttcggcc ttcgggttta gttgctccag
     5221 gctgcgtcct cgccataccc ggcccggc
//
LOCUS       DATCGS010000104         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_553919, whole genome shotgun sequence.
ACCESSION   DATCGS010000104 DATCGS010000000
VERSION     DATCGS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553919"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..683)
                     /locus_tag="VIL39_02050"
     CDS             complement(3..683)
                     /locus_tag="VIL39_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480239.1"
                     /translation="MTRKSPTARAGKKRARILLVDDHPMVRERLAEIINREADLTVCG
                     EAEDVQEALAAIPTGRPDLVIVDLTLKNSDGLELIKDVRSRWPGLRMLVVSMHDESLY
                     AERAIRAGALGYITKQEATRNILVAIRRVLTGTIYLNEKIACHIISRLTSQTGAVGAA
                     PTDLLSDRELKVFDLTGSGVSIREIANQMHIAVKTVETYRCRIKEKLKLNDSSELLQV
                     AISWNQHR"
     gene            complement(696..>2388)
                     /locus_tag="VIL39_02055"
     CDS             complement(696..>2388)
                     /locus_tag="VIL39_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5480240.1"
                     /translation="IFILDRDLKVQYVNQRGATLWRRTPKELIGLTQSELFPPEEAKL
                     HCRVVAEVFETGQAVRQDALVTYPAGEQWIEIGLVPLFGEQGTVSSVMGACRDITERK
                     RAERQLAEALDLNQKMIAAATLGIAAYKASGDCVFANNAMARITGGSISEVLQQNFRV
                     LNSWQGSGLLQLAEEALSQGQARLGEVYSTSSFGRTVWLDCHMAPFVSNGQPHLLLMV
                     LDITKRKRAEDALKQAERRQRAILDTIPDPAWLKDAEGRFLACNQALSTFYGRPVESF
                     LGKTLFDCVPPEAARSAREDTEVMTTQLSVVTEVPVADAQGRARWHESIKSPLFNERD
                     EVIGTVGIARDVTERRRLERHILEISDREQARIGQDIHDGLCQQLVSLAFDANSLQSA
                     LSDQGRPEVKTARRIADCLDQAITETRQLSRGLFPVRLETDGLPPALEELAKAAGARF
                     KIQCRFHSQGPVAVQNAAIATHLYRIAQEALTNAIKHSQARNVSIRLKAGTKQIELRV
                     EDDGAGLPPAKRTKATGMGLHIMDYRARAIGGTFHFGPGRRGGTVVSCCIPRGSS"
BASE COUNT          403 a          733 c          754 g          498 t
ORIGIN      
        1 gatcagcgat gctggttcca ggagattgcc acctgcagca gttcgctgga gtcgttgagt
       61 ttaagctttt ccttgatgcg gcagcggtag gtctcgacgg ttttcaccgc aatgtgcatc
      121 tggttggcaa tttcgcggat actcacgccg cttccggtca ggtcgaagac cttcagctca
      181 cgatctgaaa gcaggtcggt gggagcggcg ccgacggcgc ccgtctggga cgtcaaccgg
      241 ctgatgatgt ggcaggcgat cttctcgttg aggtaaatgg tgccggtcag gacccgccgg
      301 atggccacca ggatattgcg cgtggcttcc tgtttagtga tgtaacccag cgcgccggcc
      361 cggatggccc gttccgcata aagcgattcg tcgtgcatcg agactaccag catccgcagc
      421 cccggccagc gggagcgaac atccttaatc agttccaggc catccgagtt cttgagtgtc
      481 aggtcaacga tcacaaggtc gggccgcccg gtggggatcg ccgcgagggc ttcctggacg
      541 tcttcagcct cgccgcagac cgtgaggtcg gcttcgcgat tgatgatttc ggccaggcgt
      601 tcgcgcacca tcggatggtc atccaccagc aagatgcgcg cgcgcttctt gccggcgcga
      661 gcggtcggag atttgcgggt cataagcacg attctttagc tgctgccgcg agggatgcaa
      721 caggaaacga ccgtgccgcc ccgccggccg ggaccgaagt ggaaagtccc gccgatggct
      781 cgggcacgat aatccatgat atgcaggccc atgcccgtcg ctttcgtccg cttggcaggg
      841 ggtaggcccg ctccgtcatc ttcgacccgc aactcgattt gtttggtccc ggcctttaga
      901 cggatagaca catttcgggc ctggctgtgt ttgatcgcgt tggtcaaggc ttcctgggca
      961 atccgataaa ggtgcgtggc aatagcggcg ttttgcactg ccaccggccc ctggctatgg
     1021 aaccggcatt ggatcttgaa acgggcgccg gcggctttag ccagctcttc cagggccggt
     1081 ggcaaaccat ctgtctccaa ccggacgggg aagaggcccc gcgataattg gcgggtttcg
     1141 gtaatggcct ggtccaggca atccgcgatt cgccgcgccg ttttgacctc tggccggcct
     1201 tggtcggaga gcgcgctttg gagtgagtta gcgtcgaatg ccaggctcac cagttgctgg
     1261 caaagtccgt cgtgaatgtc ctggccgata cgggcctgct cgcggtcgct gatctcgagg
     1321 atatggcgct ccaggcgccg gcgttcggtc acgtcgcgag cgatgcccac cgtcccgatg
     1381 acctcgtccc gttcattgaa gagcggggat ttgatgcttt cgtgccacct cgcccggccc
     1441 tgcgcgtcgg cgacgggcac ctcggtgaca acggagagtt gcgtcgtcat tacttccgtg
     1501 tcttcacgag cggagcgagc ggcttccggc ggaacgcagt caaaaagcgt cttgcccaga
     1561 aatgactcga cgggtcggcc atagaatgtg gacagggcct ggttgcaggc caggaatcgg
     1621 ccctcggcat ccttgagcca cgccgggtcg ggaatggtgt ccaggatggc ccgttgccgc
     1681 cgttccgctt gcttgagcgc gtcttccgcc cgtttccgct tagtgatgtc gaggaccatc
     1741 agcagcaggt gcggctggcc attgctcaca aacggagcca tgtggcaatc cagccagacc
     1801 gtcctgccga agctgcttgt ggagtaaact tcgcccaacc gggcctgccc ctggcttaga
     1861 gcctcctctg ccagttgcag cagccctgac ccctgccagg agttgagcac gcggaaattc
     1921 tgttgcagca cctcgctgat gcttccaccg gtgatccggg ccatcgcatt gttggcgaag
     1981 acgcaatcgc cggaggcttt gtaggccgcg atgcccaggg ttgccgccgc gatcatcttc
     2041 tggttcaaat ccagggcctc ggccagttgc cgctcggcgc gcttccgctc ggtgatgtcg
     2101 cggcaggcgc ccatgactga actcaccgtg ccctgctcgc caaacaacgg cacgaggcca
     2161 atctcgatcc actgctcgcc ggctgggtat gtgaccagcg cgtcctggcg cactgcttgg
     2221 cccgtctcga acacctcggc cacaacccga caatggagct tcgcctcttc cggggggaag
     2281 agttctgatt gggtcagccc gatcagttcc ttgggcgtgc gccgccacaa agtcgccccc
     2341 ctttggttga cgtattggac cttgaggtcc cggtcgagga tgaagatg
//
LOCUS       DATCGS010000105         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_58725, whole genome shotgun sequence.
ACCESSION   DATCGS010000105 DATCGS010000000
VERSION     DATCGS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58725"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VIL39_02060"
     CDS             complement(<1..757)
                     /locus_tag="VIL39_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HEY5480241.1"
                     /translation="MQSTTGYRLVKLANGAHSVHSLAHHETFHPVIGPVAEAEALYVR
                     QLRLVERLQHHTGEFVLWDVGLGAAANALTALRAARDIPCSIHLLSFDHTLEPLAFAL
                     QHREALGYLVGYETHLERLLREHHCTFQDGALTVKWDMHLADFPALLAEPAARKLAKP
                     YAIMFDAFSPAKNPAMWTQPLFANLFQLLTPDRPCSLPTYSRSTMLRVTLLLAGFFVG
                     VGHATGEKEETTIAANTADLIAEPLDRRWLERVH"
     gene            complement(795..2006)
                     /locus_tag="VIL39_02065"
     CDS             complement(795..2006)
                     /locus_tag="VIL39_02065"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY5480242.1"
                     /translation="MIATSAFAANQAPVPKPDASTLAEVRQLLRDVPLIDGHNDLPWQ
                     YRKRGNDFGAINLARDTSKLTPPLITDIPRLRRGGVGAQFWAVYVPPIPCGPPAVQAV
                     LEQIDVVHQMVAHYPDTFELALTAADIERIHRQGKIASLIGMEGGHSINNSLAVLRVT
                     YALGARYLTLTHTKNTDWADAAGDQPRHHGLTQLGEDIVREMNRLGMLVDLSHVTDET
                     MRAALKVSRAPVIFSHSSVRALCDSPRNVPDDVLATTARKGGVVMVCFLPGYLTERGR
                     VAMAASEAEKARLRQLYPDSSSRFKKAMVAWHRKHPSFHEASLGDVADHIDHVRKVAG
                     IDHVGIGSDFEGFAGAPDGLEDVSCYPALLAELIRRGYTREEIRKVAGLNLLRVFREV
                     EKVAAQLKPAL"
BASE COUNT          439 a          718 c          823 g          447 t
ORIGIN      
        1 ggtggacccg ctccaaccac cgtcggtcga gcggttcggc gatgaggtcg gctgtgttcg
       61 cggcaatagt ggtttcctct ttctccccgg tggcgtggcc gacaccgaca aagaatccgg
      121 ctagcaggag cgtcacacgg agcatggtgc tgcgcgagta ggtcggcagc gaacaggggc
      181 ggtccggggt gaggagttgg aagagattgg cgaagagcgg ctgcgtccac atcgcgggat
      241 tcttggcggg cgagaatgcg tcgaacatga tcgcataggg cttggcgagc ttccgggcgg
      301 ccggttctgc cagcaaggcg gggaagtctg cgaggtgcat gtcccatttc accgtcagcg
      361 cgccgtcctg aaaggtgcag tggtgttcgc gcaagaggcg ctccaggtgc gtttcgtagc
      421 ccaccaggta cccgagtgcc tcgcggtgct gcagcgcgaa ggccaacggt tcgagggtgt
      481 ggtcgaagct cagcaggtgg atcgagcagg gaatgtcgcg agccgcgcgc agagcagtga
      541 gcgcattggc cgccgccccg aggcctacat cccacaggac gaattcgccc gtgtgatgct
      601 ggagccgctc gaccaggcgc aattgccgaa catacaacgc ctcggcctcg gccaccgggc
      661 cgatcaccgg atgaaaggtt tcgtgatggg ccagggaatg gacgctgtgc gccccgttgg
      721 cgagtttaac cagtctgtat ccggtggtgg actgcacgga aggactttcg agcacggcgc
      781 cgccaaggcc aagctcaaag agcgggcttc aattgggctg cgaccttttc gacctcgcga
      841 aagacgcgca gcaggttgag gccggccacc ttcctgatct cctcgcgggt gtagccgcgc
      901 cgtatcaact ccgcgagcag cgcggggtag caggacacat cctccaaccc gtccggcgcg
      961 ccagcaaagc cttcaaagtc cgaaccaatg ccgacgtggt caatgccggc gactttgcgc
     1021 acgtgatcaa tgtgatccgc cacgtcaccc agactggctt catggaagga gggatgcttc
     1081 cggtgccagg cgaccatcgc tttcttgaag cgtgacgagc tgtccgggta gagctggcgc
     1141 agccgcgcct tttcggcctc cgacgccgcc atagcgacgc gcccgcgctc ggtgagatag
     1201 ccgggcagga aacaaaccat caccacgccg cctttgcggg ccgtcgtcgc cagcacgtcg
     1261 tccgggacgt tgcgcgggga atcgcacagg gctcgcacgg aggaatgcga gaaaataacc
     1321 ggagcccggc tgactttcag cgcggcgcgc atggtctcat cggtgacgtg cgagaggtcc
     1381 accagcatgc cgaggcggtt catctcgcgc acgatgtctt cgccaagctg ggtcagaccg
     1441 tgatgcctgg gctggtcgcc cgcagcatcg gcccaatcgg tgttcttggt gtgagtcagc
     1501 gtcaggtaac gcgcgccgag ggcataggtc acgcgcagca ccgccagcga gttgttgatc
     1561 gagtggccgc cttccatgcc gatcagagaa gcaatcttcc cctgacgatg gattcgctcg
     1621 atgtcggcgg ccgtcagcgc gagttcgaag gtgtcgggat aatgggccac catttggtgg
     1681 acgacatcaa tttgctccaa caccgcctgc acggcgggcg gcccgcaggg gatgggagga
     1741 acgtacaccg cccagaactg ggcgccgacg ccgccgcgcc gtaagcgcgg gatgtccgtg
     1801 attagcggcg gggtgagttt gctggtgtcg cgggccaggt taatcgcgcc gaaatcgttg
     1861 ccgcgcttgc gatattgcca tggcaggtcg ttgtgaccgt caataagcgg cacgtcgcgc
     1921 agcagttggc ggacttctgc cagggtggaa gcatctggtt tcggcactgg tgcctggttg
     1981 gcagcaaagg cgctggttgc gatcatagaa agcgtgatag ctccgcgttt ggagcgggtg
     2041 ctcatgcagg cgccagcttg gggttggacg cgctcgcggt caagtcgccg cgccgcgggc
     2101 gcatttcatt tcatacacgt cgcgggattc tccacagact gcgccgtccg gaggacccgc
     2161 gcgtccttgc cctccggctt ccgcgacgct gacctcgggc ctctggccag cgtgatgcct
     2221 aggcattgct agcgtgctgc tagctggttg cccgcctgat gcttcgaagg agttacgaag
     2281 gagttacgaa ggagttacga aggagttacg atggggttac gatggggtta cgatgggatt
     2341 caaggaaaac cacctcctca ttacagtccc tcgaccgtct cggcaacgcc aagccaagct
     2401 gcctccggcg tctcttttgt gaattgg
//
LOCUS       DATCGS010000106         5796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_652592, whole genome shotgun sequence.
ACCESSION   DATCGS010000106 DATCGS010000000
VERSION     DATCGS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652592"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..572)
                     /locus_tag="VIL39_02070"
     CDS             complement(291..572)
                     /locus_tag="VIL39_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5480243.1"
                     /translation="MSKSKQRANGSMELGRYIVADPMICHGRPTFKGTRVFVADALAD
                     VERGLSWDFIVRRWGGGKITKSAITEAVRLARSALLRDDGRLASLSTAA"
     gene            complement(991..4140)
                     /locus_tag="VIL39_02075"
     CDS             complement(991..4140)
                     /locus_tag="VIL39_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5480244.1"
                     /translation="MKSFTDLFIRRPVLALVVNLVILIAGFQAIRTLVVRQYPRSDNA
                     AVTVTTVYVGASADLVRGFITTPLERAIAAADGIDYIESSSKLSLSTITIRLKLNYDP
                     TKALAEISSKVDQVRNDLPPEAEVPIINVTSADSEFAAAYLRFSSDVLEANQITDYLV
                     RIVQPRLSALEGVQRADILGGRTFAMRIWLKPDSMAALKINPAQVREALAANNFLSAV
                     GRTKGSLVQVNLSANTDLRSIEEFRNLVVRRSGDNLVRLRDVADVVLGAEDYDSEVRF
                     GGAKAVFMGVWPLPNANSLDVMKRVRAEMVLIQKELPSGLTAGIAYDATQYIEDAIHE
                     VLKTLTETLLIVAIVIFLFLGSLRSVLVPLVAIPISLIGAIFLMQVFGFTLNLLTLLA
                     IVLSVGLVVDDAIVIVENVERHIREGRTPRQAALLGARELVGPVIAMTITLAAVYAPI
                     GLQGGLTGSLFREFAFTLAGAVFISGVVALTLSPVMSSKLLDAGREEHGLTGRINRGF
                     DRLRRAYGRVLDGTLAARPAVYIVWLVLGLLSIPLFILAPKELAPMEDQGFVMGIVEA
                     AADATIDQTTFYTEAVNREMLSLPEAYQTFQITFPDNAFCGLVLKPWGERKRTVFTLT
                     PEVQRKVSSIPGIRAFVATPPALPGGGQFPVEFVIGATAEPDVILKFAEQLQQRAATN
                     GMFAFPPIIDTKMDQPEMELVMDRDKVAALDLNMATVGADLSTLVGGNFVNRFSLAGR
                     SYKVIPQVKRIERLNANQLENTYVKGPNGQLVPLSTFATLHKKTGPRSLNRFQQFNAV
                     KISGVAIRPLDEALTYLETEAAKILPKGYKIDYTGESRQLRVEASKFLPAFTLALVLI
                     FLVLAAQFNSFRDPLIILLGSVPLAMFGALIFSFLKMPGQGMPFFTNGWTTTLNIYSQ
                     VGLVTLIGLVSKNGILIVQFANELQRTGLPKLQAVREAALIRLRPVLMTSAATICGHF
                     PLTLVSGAGAAARNSIGITIVAGMALGTLFTLLVIPSIYVLIAKEHAGEERRALDSDL
                     DLPELAQEDARQAVEAAAK"
     gene            complement(4157..5287)
                     /locus_tag="VIL39_02080"
     CDS             complement(4157..5287)
                     /locus_tag="VIL39_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480245.1"
                     /translation="MIRKILIAVFVVLLVVGALAGVKMLQIKKLIASGKSFVVPPETV
                     SSALVREEKWQGTLTAIGSVTAVQGVTVTPEIAGIVHEITFESGATVSKGDLLVRLDT
                     STEEAQLRALEAQEELARLNLARERTLRTQNMVPQSELDTAEATLKQTQGTADATRAI
                     IQKKTIRAPFAGRLGLRLINLGQYLETGKPIVSLQSLTPVYAEFTLPQQELSRLKTGM
                     HVNVTTDTYPGRKFEGTLTAINPDLDAQTRSVGLQATFENPEQLLRPGMFARVEVMLP
                     EEQAVLVVPATSVLSAPYSDSVYVIEADPGKEGGKPGLVVRQQFIRSGTARGDFVSVK
                     SGLKGGERIVSAGIFKLRNGMSVIENNDLSPKNDLAPRPSDS"
BASE COUNT         1082 a         1802 c         1866 g         1046 t
ORIGIN      
        1 cgaaattgtt cgcgagctgc gaggattgtt gtggatgaac tgccacgcga aacgtttgga
       61 gcgcgcccag cttgcgggcg ctatcgtaag gcgggggcgg ctcgaaagcg ggagcaagct
      121 ccacgcactc caaacgcttc gcgctgccaa agctggagca acacctttgg cgaaactccc
      181 cggccctggg gggcttcacc agttgccggg ccaaactgcc cgctggcgag ccacacggtt
      241 tctctgccag aattcgagac cgtacagcag gatattttca tccagcagga ttatgcggcg
      301 gttgaaagcg aggccagacg tccgtcatcg cgcaagaggg cgctgcgggc caggcgcacg
      361 gcctcggtga tggcggactt ggtgatcttg ccgccacccc accgccgcac aatgaagtcc
      421 caggacaacc cgcgttccac atcggccaac gcgtcggcga cgaaaacgcg cgttcctttg
      481 aacgtcggtc tgccgtggca gatcatcggg tcggcgacaa tatatcgccc cagttccatg
      541 cttccgttgg cgcgttgctt ggacttgctc atggtgaaaa gttacacgcc cgaggcggga
      601 ctgacgaggc gttcccggcg ttcccggcgt cggtattgag aaagcccgtg ccgcatacca
      661 tgtcatggcg tgcaagcggg catagaacag gttgatgtcc tcagacaatt tccaagcggc
      721 ttccggcgta agccggctgc ggcggacgac gctctcgacg cggccgcctg cgtgatcgac
      781 tgtctgccca acatggttgc cggtctgcta cgcgccgagc gacgttctgc tgggtggcga
      841 cgggatataa tttggcacca tgtccggccg caacggcggg atgtttgcgg cctcatttgc
      901 cgacacaaaa ggcgtttaat catgaaatgc tgtaggctac gctccatcga agggcaaaaa
      961 tgtccatcta aaagggctgg caaccggttc ctacttcgcc gcagcttcga cggcttgacg
     1021 agcgtcttcc tgggccaact ctggcagatc gaggtcgctg tcgagggctc tgcgttcttc
     1081 gccggcatgc tccttggcga tcaaaacgta gatcgaagga atcaccagca gggtgaacag
     1141 cgtgcccagc gccatgccgg cgacgatggt aataccgatc gaattccgcg cggccgcgcc
     1201 ggcaccgctg accagcgtga gcgggaagtg accgcagatc gtggccgcgc tggtcatcag
     1261 cacggggcgc aggcggatca aggcagcctc gcgcacggcc tggagtttgg gcagaccggt
     1321 gcgctggagt tcgttggcga attggacgat caggatgccg ttcttcgaga ccagcccgat
     1381 gagggtcacc agcccgacct gggagtaaat gttcaacgtg gtggtccagc cgttggtaaa
     1441 gaacggcatg ccctggccgg gcattttcag gaagctgaag atcagcgcgc cgaacatggc
     1501 caggggcact gagcccagca ggatgatcag cggatcgcgg aagctgttga actgggcggc
     1561 cagcaccagg aagatcagca ccagcgccag cgtgaaggcg ggcaggaatt tgctggcttc
     1621 cacgcgtagt tggcgggatt cgccggtgta gtcaatcttg tagcccttgg gcaggatctt
     1681 cgccgcctcc gtctccaggt aggttagcgc ctcatcgagg gggcggatgg ccacgccgct
     1741 gatcttgacc gcgttgaatt gctggaagcg gttgagcgag cgcggcccag tcttcttgtg
     1801 cagcgtggcg aaggtgctca ggggcaccaa ctgcccgttc ggccccttga cgtaggtgtt
     1861 ctcgagctgg ttggcgttga gccgctcgat gcgcttgacc tgcgggatca ccttgtagct
     1921 gcggccggcc aggctgaacc ggttaacgaa gttgccgccc accagcgtgg acaggtcggc
     1981 gcccacggtc gccatattga gatccaacgc ggccaccttg tcgcggtcca tgaccagttc
     2041 catttccggc tggtccatct tggtgtcgat gatcggcggg aaggcgaaca tgccgttggt
     2101 cgccgctcgc tgctgcaatt gctccgcgaa cttcaggatc acatccggct cggcggtggc
     2161 gccgatgacg aattccaccg ggaactggcc gccgcctggc agcgcgggcg gggtggccac
     2221 gaaagcgcga atgcccggga tactgctgac tttgcgttgc acctccggcg tcaacgtgaa
     2281 cacggtgcgc ttccgctcgc cccagggctt gagcaccagg ccacagaagg cattgtccgg
     2341 gaaggtgatc tggaaagttt ggtacgcctc cggtaggctc aacatctcgc ggttgaccgc
     2401 ctccgtgtaa aaggtcgttt ggtcgatcgt cgcatccgcc gccgcctcga cgatgcccat
     2461 cacaaagccc tggtcctcca tcggcgcgag ttctttgggg gccaggatga acagcgggat
     2521 ggacagcagc ccgagcacca gccagacgat atagaccgcc ggacgggccg cgagcgtgcc
     2581 gtcgagcacc cggccgtaag cccggcgcaa gcggtcgaag ccgcggttga tgcggcccgt
     2641 caggccgtgc tcctcgcgcc cggcatcgag cagcttggac gacatcaccg gggaaagggt
     2701 gagggcgacg acgccggaaa tgaataccgc ccccgccagc gtgaaggcga attcgcggaa
     2761 tagcgacccg gtcaggccgc cctgcaggcc gatgggcgca tagaccgccg ccagcgtgat
     2821 cgtcatggcg atgaccgggc ccaccaactc gcgcgcgccc agcagggcgg cctgccgggg
     2881 cgtccgcccc tcgcggatgt ggcgttccac gttctcgaca atgacaatgg cgtcgtccac
     2941 cacgagcccg accgagagca cgattgccag cagggtgagc aggttgagtg tgaacccgaa
     3001 cacctgcatc aggaagatgg caccgatcag ggagatcggg atggcgacca gcggaaccag
     3061 taccgagcgg agcgagccga ggaacaggaa gatcacgatg gccacaatca gcagcgtctc
     3121 ggtcaaggtt ttcagcacct cgtggatggc atcctcgata tactgggtcg cgtcgtaggc
     3181 aatgccggcg gtcaggccgc tgggcagctc cttctggatg agcaccatct ccgcgcggac
     3241 ccgcttcatg acatccagcg agttggcgtt gggcagcggc cagacgccca tgaacaccgc
     3301 cttggcgccg ccaaaccgga cctccgagtc gtaatcctcc gcgccgagca ccacgtcggc
     3361 cacgtcgcgc agccggacga ggttgtcgcc cgaccggcgg acgacgaggt tgcggaactc
     3421 ctcgatcgag cgcaggtcgg tgttcgcgct gaggttcacc tgcaccagcg agcccttggt
     3481 tcggccgacg gcggagagga agttgttggc ggccagcgcc tcgcggacct gggcgggatt
     3541 gattttcaac gccgccatgc tgtcgggctt gagccagatg cgcatggcaa aggtccggcc
     3601 gcccaggatg tcggcgcgtt gcacgccttc cagtgcggag agccggggct ggacaatgcg
     3661 aaccaggtaa tcggtgatct ggttcgcctc cagcacgtcc gaggagaagc gaaggtacgc
     3721 ggcggcaaac tcggaatcag cggaggtgac gttgataatg ggcacctcgg cttccggcgg
     3781 caggtcgttg cgcacctggt ccaccttcga gctgatctcg gcgagcgcct tggtcggatc
     3841 gtagttgagc ttgagccgga tcgtgatggt ggacaggctg agcttgctgc tcgactcgat
     3901 gtaatcaatg ccatcagcgg cggcaatcgc cctctccagc ggcgtggtga tgaacccgcg
     3961 caccaggtcc gcgctggcgc ccacatacac cgtggtgacc gtcaccgccg cattgtcgct
     4021 gcgcgggtat tgccggacca ccagggtgcg gatcgcctgg aacccggcga tcaagatcac
     4081 caggttcacc accagcgcga ggacgggccg ccggatgaat agatcggtaa aggacttcat
     4141 ggacgcgatc cggggctcaa ctatccgacg ggcggggcgc caggtcgttc ttcggcgaca
     4201 ggtcgttgtt ctcgatcacc gacatgccgt tgcgcagctt gaagatgccg gcgctgacaa
     4261 tgcgttcgcc gcccttgagg cccgatttga cgctcacaaa atccccgcgc gccgtcccgc
     4321 tgcgaatgaa ctgctgccgc accaccaacc ccggcttccc gccctccttg cccgggtccg
     4381 cttcgatcac atacaccgag tcgctgtagg gcgcgctcag gaccgacgtg gcggggacca
     4441 ccagcaccgc ctgctcttcc ggcagcatca cttcgacgcg ggcgaacatg cccggccgca
     4501 gcagttgctc gggattctcg aaggtggcct gcaaccccac gctgcgcgtc tgcgcgtcca
     4561 gatccggatt aatggccgtg agcgtgccct caaatttccg gcccggatag gtatcggtcg
     4621 tcacgttcac atgcatgccc gtcttaagcc gcgacagctc ctgctgcggg agcgtgaatt
     4681 cggcatacac cggggtcagc gattgaagtg agacaatcgg tttgcccgtc tccaagtact
     4741 ggcccaaatt gatgaggcgg aggcccaggc gaccggcaaa gggcgcgcga atggtcttct
     4801 tttggattat ggcgcgggtg gcatcggccg tgccctgggt ctgtttcaag gtcgcctcgg
     4861 cggtgtccag ttcggattgc gggaccatgt tctgggtgcg cagggtgcgc tcgcgggcga
     4921 ggttgaggcg ggccagctct tcctgcgctt ccagcgcccg cagttgagcc tcctcggtcg
     4981 aggtgtccag gcgcaccagc aggtcgccct tggacaccgt cgcaccggat tcaaaggtga
     5041 tctcgtgaac gatgccggcg atttccgggg ttacggtcac cccctggacg gcggtcactg
     5101 aaccgatggc cgtgagcgtg ccctgccatt tctcctcgcg caccaaggcg gaggagacgg
     5161 tttccggcgg cacgacaaag ctcttgccgc tggcaataag cttcttaatc tggagcatct
     5221 tcaccccagc cagggcgccg acgaccagaa gcactacaaa aaccgcgatc aatatcttac
     5281 gtatcatcag tggacaaaag agcgacccat ggtcgcccgg ttaacagttt tagctacaaa
     5341 cgtcacaaat attagggctg acggggcgcg cggtcaaaca agacctgacc cgccccagcc
     5401 ggcgcccgta acgggtcaat gacggtggag gccgcgttgg cggagtgcgc ccgtcctcgg
     5461 gcgcagcaat caccgaccag gctgacgtgc ccaatttcat tgcatccccg tcgctttccg
     5521 acattgctac gcccgaggac gggcgctctc cgccagggta ttctcccgcc ctcactgccc
     5581 cactactggc aagcggtgcc cagacgaaga ggatgcgaca tggaggcggg aaaggcccct
     5641 cccaggcacc gtgcaagtgt cagtatagaa gggggttttc agcagaatca gccctgtctg
     5701 gtgatccctt tcccctgaac ttgcttcagc aaatcaccgg cacgccgtat ttttcaaact
     5761 gttcgtcagt cgttaccacc gtcaagtcgt gcagtt
//
LOCUS       DATCGS010000107         5189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_653001, whole genome shotgun sequence.
ACCESSION   DATCGS010000107 DATCGS010000000
VERSION     DATCGS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653001"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..623
                     /locus_tag="VIL39_02085"
     CDS             279..623
                     /locus_tag="VIL39_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480246.1"
                     /translation="MKNTTNCLLMVLLAGGLAFAGCGKPASTQVQGTTIAAMDVPKFQ
                     QAFPSPTPAQQSSIDKVRMGVRYGTYPDVLAALDKLASDPSLTEPQKKAVSDLIEGVK
                     QTLAKAATAPAK"
     gene            complement(657..1643)
                     /locus_tag="VIL39_02090"
     CDS             complement(657..1643)
                     /locus_tag="VIL39_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5480247.1"
                     /translation="MAETSNKGEYLVGVDLGGTKILAGVFKNSIECIGTSKLSTKPQR
                     GVDAVIERIARCVQDAVDEADLSLKQVAGIGIGAPGAVDFADGRVIFAPNLEGWKDVP
                     LKKNLEKALGVPVFVENDCNICALGVHVAELKSKPKSLIGIFVGTGIGGGLIINGELY
                     GGFNHTAGEIGHMVLDINGPKCGCGNKGCFEALASRSAIFQQIKAGVKDGEKTMLTEM
                     LGDDLADLRSGDLRKAIRRGDKFVDKVIEGAAEYIGIGIANLVNILSPEVVVLGGGVI
                     EALADEMMSVIVETAQDYAMPGTMEGVEIIASKLGDSAGITGGAVMARKETK"
     gene            complement(1653..2384)
                     /gene="tmk"
                     /locus_tag="VIL39_02095"
     CDS             complement(1653..2384)
                     /gene="tmk"
                     /locus_tag="VIL39_02095"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5480248.1"
                     /translation="MKSVAKNYIVVPRRSERRFYGQGIPGVDLEQMAGKLIAMEGADG
                     SGRSTQIAMLVEWLEGSGHATVQVGLKRSTLVSEELETAQEGNILSRTTLSLFYATDF
                     ADQLENIILPALKAGFIVLADRYIYTLMARDMVRGMDEVWLKNLYGMALEPDAVFYLN
                     VSPEELVQRTFAKNAALDYWESGMDQGLSRDMFDSFMKYQTAMQKTFRRLQSSYDFTI
                     VDGMRPVQVINAELQKKISALLAGK"
     gene            complement(2381..3124)
                     /locus_tag="VIL39_02100"
     CDS             complement(2381..3124)
                     /locus_tag="VIL39_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEY5480249.1"
                     /translation="MKTFAELGFPGRLIAVEGLDGSGKSTQIYLLKRWLELQDLKVYF
                     SEWNSSELVKAATSKGKKRELLTPTTFSLIHATDFADRYERHLVPLLRAGYIVLCDRY
                     IFTAFARDVVRGCPPEWVRGIYNFASLPDLTFFFKSDLEVSLRRILDGRPQLKYFEAG
                     MDLHLSTDPYESFRIFQGRILEQYLAMSTEFNFLVLDANQVIEKQQGVVRELISKKLD
                     LASFQRAGRPSAKQPAAPGKKLQEEEPVE"
     gene            complement(3199..4653)
                     /locus_tag="VIL39_02105"
     CDS             complement(3199..4653)
                     /locus_tag="VIL39_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5480250.1"
                     /translation="MILNLIFGSLALLSLALTLWQWLVARRFPLHQRVAEPASPPDPH
                     RSRREEDLTAPPQPSTLNPQPPLTLLKPLKGCDPATEDCLRSWFAQQYTGPVQILFGV
                     AAADDPVCGIVRRLLQEFPGSDAQLVVGIPLPGANAKVAKLAELESLAKHDLLVISDA
                     DVRVPSDFLVNIVTPLCEGDLECGGKRGAPPLWLQRQIEKAMPKRRRRCALSTHSIRP
                     LTVLDSGAQCGISKSGAQACGLVCCFYRLANPTTLAMQWEAIAINADFWSQVLQSQSL
                     KPLDFALGAVMATRRQQLQEIGGFAGLVDCLADDYQLGNRIARRGHAIALSPVVVDCW
                     SAPMGWADVWKHQLRWARTIRVCQPVPYFFSLLSNATLWPLLWLIIKPAAPVAACAIF
                     CWLARIVTALDFQRRLGQYPGPGGRASPRAQSDRTPEAPSQAPWHYAWLIPVKDLLQA
                     AIWLLAFLGNRIEWRGQQMRLRRDGTLERLNHSR"
BASE COUNT          999 a         1543 c         1668 g          979 t
ORIGIN      
        1 ggctgcagtt tctcgttttg tgatggccat gccgagatcc ataaatggag gggcaacgcc
       61 atcaaagtga acgcggcgaa ccctccccaa cacaccgcga ccagcccttt ggaccaggtt
      121 gactggtggt ggcttgcgaa cgcttcctcg gtcaaaatca attagaagtt tcgagttcgg
      181 gaccggctcc cgacgatggt acagtcaagg acctgatagg gtggtccacg attacgacca
      241 tgcgtaaata gttgcgtata agaaacatta caaactgtat gaagaacact acaaactgcc
      301 tgctcatggt cttgctggcc ggcggtctcg ccttcgccgg gtgcggcaag ccagctagta
      361 cgcaggtgca gggcaccaca atagcggcga tggacgtccc gaagttccag caggctttcc
      421 cgtcccccac gcccgcacaa caaagcagca ttgacaaggt acggatgggc gtgcgctacg
      481 gcacgtaccc ggacgtcttg gcggctttgg acaaactggc gagcgaccca tccctcactg
      541 agccgcagaa gaaggccgtt agcgacctga tcgagggcgt caagcagacc ctggccaagg
      601 ccgccacagc gcccgccaag taaccccgct cggggcagtg cgcccaggcg ggccgcttac
      661 ttggtctctt tgcgggccat cacggcgccg ccggtgatgc cggcgctgtc gccgagcttg
      721 gatgcgatga tctcgacgcc ttccatcgtg ccgggcatgg cgtagtcttg cgcggtttcg
      781 actatcacgc tcatcatctc gtcggccagc gcttcgatga cgccgccgcc gagtacgacg
      841 acttcggggc tgaggatgtt gacgaggttg gcgatgccga tgccgatgta ctcggcggcg
      901 ccttcgatga ctttatccac gaacttgtcg ccgcggcgga tggctttgcg gaggtcgccg
      961 ctgcggaggt cggcgaggtc gtcgcccagc atttcggtga gcatggtctt ctcgccatct
     1021 ttgacgccgg ccttgatctg ctgaaagatg gcgctgcggc tggcgagagc ttcgaagcag
     1081 cccttattgc cgcagccgca tttggggccg ttgatgtcga ggaccatgtg gccgatctcg
     1141 ccggcggtgt ggttgaagcc gccgtagagt tcgccattga taataaggcc gccgccgatg
     1201 ccggtgccga cgaagatgcc gatgaggctc ttgggtttgc tcttgagctc ggcgacatgg
     1261 acgcccagcg cgcagatgtt gcagtcgttc tctacgaaca ccggcacgcc gagagccttc
     1321 tcgaggttct tcttgagggg cacatctttc cagccctcca ggttgggggc gaagatgacc
     1381 cggccgtcgg cgaaatccac cgcgcccggc gcgccaatgc cgatgccggc gacctgtttg
     1441 agcgagaggt cggcttcgtc caccgcgtcc tgaacgcagc gggcgatgcg ctcgatgacg
     1501 gcatccacgc cgcgctgggg tttagtgctg agcttggagg tgccgatgca ctcgatcgag
     1561 ttcttgaaga cgcccgccag gatcttggtg ccgccgaggt caacgccgac gaggtattct
     1621 cctttgttgc tggtttcagc cataattctc tcctatttcc cggccagcag ggcgctgatc
     1681 ttcttctgca gctcagcgtt gatgacctgc accgggcgca ttccgtccac gatggtaaaa
     1741 tcataagagc tttggagccg gcggaacgtc ttctgcatgg ccgtctggta tttcatgaag
     1801 ctgtcgaaca tgtcgcggga cagcccctgg tccatgccgc tctcccaata atccagggcg
     1861 gcgttcttgg caaaggtccg ctgcacgagt tcctcgggcg agacgttgag gtagaacacc
     1921 gcgtccggtt ccagcgccat gccatacagg ttcttcagcc agacctcgtc catgccccgg
     1981 accatgtcac gcgccatcag cgtataaatg tagcggtcgg ccagcacgat gaagccggcc
     2041 ttgagcgccg gcaggatgat gttctccagt tggtcggcga aatccgtcgc gtaaaacagg
     2101 ctcagggtgg tgcggctcag gatgttcccc tcctgcgccg tttcgagttc ctcgctgacg
     2161 agggtggagc gtttgagccc cacctgcacg gtggcatggc cgctgccctc gagccattcg
     2221 accagcatcg caatctgggt ggagcgcccg gagccgtccg cgccttccat cgcgatgagt
     2281 ttgccggcca tttgctccag gtcaacgccc ggaatcccct gcccgtagaa gcgcctttcg
     2341 ctgcggcgcg ggacgacgat gtagttcttg gccacgctct tcattcgacc ggttcctcct
     2401 cttgaagttt cttgccgggc gcagccggtt gtttggcgga cggacgtccc gcgcgttgga
     2461 agctggccag gtccagcttc ttcgagatga gttcgcgtac gacgccctgc tgcttctcga
     2521 taacctggtt cgcatcgagc accaggaagt tgaactcggt gctcatagcc aagtactgct
     2581 ccaggatgcg cccctggaag atgcgaaagc tctcgtaggg gtcggtggac aggtgcagat
     2641 ccatgcccgc ctcgaaatac ttcaactggg gccggccatc caggatgcgc ctgagcgaca
     2701 cctccaaatc ggacttgaag aaaaaggtga ggtcgggcag ggacgcgaag ttgtagatgc
     2761 cccgcaccca ttcgggcggg cagccgcgga cgacgtcgcg cgcaaaggcg gtgaagatgt
     2821 agcggtcgca taggacgatg tagccggccc gcaacagcgg caccaggtgc cgttcatagc
     2881 ggtcggcaaa atccgtcgcg tgaatgaggc tgaacgtggt cggcgtgagc agctcgcgtt
     2941 tcttgccttt gctggtggcg gctttgacca gctcggagga attccactcg ctgaaataga
     3001 ccttcaggtc ctgcaattcg agccagcgtt tgaggaggta aatctgcgtc gatttccctg
     3061 agccatccag gccttccacc gcgatgagcc ggccggggaa accgagttca gcgaatgttt
     3121 tcattaataa cgtccgtagg ctaagcccgc ctttgcgaaa cacaactgtt ttctcggccg
     3181 caagccagga cggcagggct acctcgagtg gttcaaccgt tcgagcgtcc cgtcgcggcg
     3241 gagccgcatt tgttgcccgc gccattcaat acggttgccg agaaatgcga ggagccagat
     3301 ggccgcttgg agcagatcct tgaccgggat gagccaggca tagtgccagg gcgcttgcga
     3361 gggcgcttcc ggggtgcggt cagattgggc tcgtggggac gctcgccctc ccgggccggg
     3421 gtattggccc agtcgccgct ggaaatccag ggcggtcacg atccgggcga gccagcaaaa
     3481 gatggcgcaa gcggcgactg gcgcggcggg tttgatgatg agccagagga gcggccagag
     3541 tgtggcgttg ctcaggaggc taaagaagta gggcacgggt tggcagacgc gaatggtgcg
     3601 cgcccagcga agctgatgct tccagacatc ggcccagccc atcggcgccg accagcagtc
     3661 caccacgacc ggcgagaggg cgatggcgtg gccgcggcgc gcgatgcggt tgcccaactg
     3721 atagtcgtcg gccaggcaat ccaccaaccc ggcgaagccg ccgatttcct ggagctgctg
     3781 gcggcgggtg gccatcacgg cgccgagagc gaagtcgagg ggcttcaggc tctgggactg
     3841 caagacctgg ctccagaagt cggcattgat ggcgatggct tcccactgca tcgccagcgt
     3901 ggtggggttg gccaggcgat agaagcagca taccaggccg cacgcttgcg ctcctgactt
     3961 ggatattccg cactgcgccc ctgaatccag gaccgtcagc ggtcttatgg agtgcgtcga
     4021 cagagcgcag cggcgacggc gctttggcat ggctttctcg atctgccgct gaagccaaag
     4081 cgggggcgcg cctcgcttgc ccccgcattc caaatccccc tcgcacagag gtgtgactat
     4141 attgaccagg aaatcggagg gcacgcggac atcggcgtcg ctgatgacga gcaggtcgtg
     4201 tttggccagg ctttcgagct cagccagttt ggcgaccttc gcattcgcgc cgggcagagg
     4261 gatgcccacc accagttgcg cgtcgcttcc cgggaactcc tgcaaaagcc tgcggacgat
     4321 cccgcacacc gggtcgtcgg ccgcggctac gccgaagagg atttggacgg ggccggtgta
     4381 ctgctgggcg aaccagcttc gcaaacagtc ctcggtcgcc gggtcgcagc ctttgagggg
     4441 cttgagcaga gtcaagggag gttgagggtt gagagttgag ggttgagggg gagcagttag
     4501 atcctcctca cgtcggcttc tatgaggatc aggaggcgag gcgggctcgg ccacgcgctg
     4561 atgcagggga aaacgccggg caacaagcca ttgccagagt gtcagtgcca ggctcagcag
     4621 ggcgagggag ccgaaaatca ggttcaggat cactgcttgt tgtgtgtggt cgtcgtccgc
     4681 gcaatcgtaa tcgagccggc gaggggaagg aaatggaaat ccgaagcccg aaaactgaag
     4741 gaaacccgaa ggccgaaatc cgaacgccga gggggtgaat ggatcgagag ttcattcggg
     4801 aggtgggtgc cggaatgaga agcggattgt ctggcgggcc cctgacaggc cgaaggggga
     4861 gcttgcgccg ccaaagcgcc gtagcccctt cgggcgcatt acgaagcaat atacgcatgg
     4921 gccgaaaacc ctatgaacaa ccctgcgctg accagcagca gttctcatta tggcgattcc
     4981 gctcggaatc tccgccaaac ccgatctcct gcagctccag agaccagtcc aagtcgggag
     5041 cgaacccgag ctcacccacc gggattcttc acgaaagagg aaacaaacgc cagcgcgcct
     5101 aaatgcgagc ttgatgcagc ccagagccac ccaaggccac ctcaaggcca cctcaaggcc
     5161 accacagagc catatactct ggagtacag
//
LOCUS       DATCGS010000108         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_306996, whole genome shotgun sequence.
ACCESSION   DATCGS010000108 DATCGS010000000
VERSION     DATCGS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306996"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VIL39_02110"
     CDS             <1..484
                     /locus_tag="VIL39_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5480251.1"
                     /translation="LGNTQGAADKPWFWTQPPACFGSYTYNGWLYSGATTYGDPVKYF
                     KKESAIQKPSQTPAFADGIWVDAWPLVTDKPASPCDLYDGADINSYQGMPRFLIGRHG
                     KGPATGAPRNISPAFTPVLPGKIDVGCFDGHVELVPLDNLWSRFYWHIDYAPASRPRP
                     "
     gene            609..2975
                     /locus_tag="VIL39_02115"
     CDS             609..2975
                     /locus_tag="VIL39_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480252.1"
                     /translation="MNSKRPQRPATGLWSGPRRAAAVLCLCCAAAFLARGSERASGAK
                     RQREPAFTTSAGSAQIKAGILTRRFGFKDGNLATTSLAIDGHESLAGPARELSFAVSF
                     AEPNQRPRGIRADEYAAVETTAHFSSGTDALRIEGGREQAGVAWSPNLAVRANAWSAY
                     FEPVACRIRQSGTHVAAGVSPAAATGSGLLVITAQASSSSPLKGLTVRLCYEVYDGFP
                     VIRKWVEITNGGTRWLKLNKLVIDDLELAADCRHQTPLTPGERGAGPSVIAFGTPDGR
                     RGVVAVSEIPSALRETSDTGAMGYSEELFEWVLGPGETFISEPVFFLAYSGELHKTIS
                     AISTPRDRTVEGVYLDFLRKQVGVAADKGTIETPQWCSWSNFGWTLNDGIVRQQAELA
                     ARCGFALLLLDSGWQKDTIGTEADATRFPDFAATCRHIRSLGLKLGLWVSCFRVADSP
                     DLRAKPELRSAPLIKRDGGFGMSFASPWKEFYAKDLARISHKYGVSYFKQDYTNVKLG
                     DSAEGHESRTHRESLLRGLRGLLVAQDLLRQSAPQVAPEVTHEIYWGTPGVPCDVAVV
                     KHAVAYHIPPNDYAGVGHPKERVRTNWPYEPAKLRQQLLDGCFNARQRFYAHRGLPLY
                     ALEYYAAHTVNFRGSLTPAVQQRQVCSWLMGVPSTFAGDLASLTEENIACYRRLFTLL
                     QRLEKDYGIYRHFQFSGAPAPTDTDWHWWGKLNGQGCGAVVVLRGSQGADERAVNIPW
                     VLPGCTYQVNGLLAHQSLGRFSGKELQQGALVLKLAPFGQEILECKAE"
     gene            2972..4348
                     /locus_tag="VIL39_02120"
     CDS             2972..4348
                     /locus_tag="VIL39_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY5480253.1"
                     /translation="MNFRFVFFLCFTAALGGLLFGFDIAIITGAGPFLLQHFKLDDLS
                     LGWAFSSLLFGCILGSVIAGRLTDLVGRRRVLLAVAGLFALTTVATGLSPSFSWFILA
                     RFLGGLAVGGASIVSPMYVAEIAPANLRGRMCAFYQLSIVIGILASYCINFLLRDAGL
                     WNWRWMFISGVIPSIVFLLMLVRAPETPRYLFKCGRQAEATALLCRLVGPEQAAREIA
                     EIQASLAEHHAGWRELLHPGLRRVLAVGFVLAVLVHFSGINTIIDYAPIILQSAGLKV
                     DAALFSTFIIGLTNFLFTLVSFWTIDRFGRRPLYITGSLGMTVVLMLLAVASVLNRFT
                     GVIALGLIMAYIAFFASCIGPVFWTLVPEIFPNRIRGTAMSVPVLTQWAANAVVVLFF
                     PLAFNHIGKAPTFGFLGIMALAQALFLWRFVPETKNMTLEQIEEIWKNAGNRLTGVDK
                     KEHDKVSR"
BASE COUNT          750 a         1348 c         1329 g          929 t
ORIGIN      
        1 tttaggcaac acccagggcg ccgcggacaa gccctggttt tggacccaac cgccggcttg
       61 cttcgggagc tacacctaca atggctggct ctattccggc gccactacct atggcgaccc
      121 ggtcaaatac ttcaagaagg aatcagccat ccaaaagccc tcccaaaccc cggccttcgc
      181 tgacggaatc tgggtggatg cttggccttt ggtcaccgac aagccggcct cgccttgcga
      241 tttgtatgac ggcgccgata tcaatagcta tcagggcatg ccgcggtttc tcatcgggcg
      301 gcacggcaaa gggcccgcca ccggcgcgcc ccggaatatt agcccggcct tcaccccggt
      361 cctgccgggc aagattgatg tgggatgttt cgatggccat gtcgagctgg tgcctctgga
      421 caacctttgg tcgcggttct actggcatat agattacgcg cctgctagcc ggccccgccc
      481 ctgaccaatc gtccgatgcc gccgcgctac cctcatgacg ccaacacctc agcggcgcac
      541 caggcagttg agtgaaccat tttccagggc tggaagtcct caagataatc tctaagctgc
      601 tgaaacagtt gaacagcaaa agacctcaac ggccggcgac ggggctgtgg tccggcccac
      661 gacgggcagc ggcggttctt tgtctctgct gtgctgccgc tttcctggcc cgcggcagtg
      721 agcgagcctc tggggcaaaa cgtcaaagag aacctgcctt caccaccagt gccgggtcgg
      781 cccagatcaa ggccggcatc ctgacccgga ggttcggctt caaggacggc aaccttgcca
      841 ccaccagcct cgcaatcgac ggccacgaaa gcctggccgg ccccgcgcgc gagctctcat
      901 ttgccgtttc attcgcggag ccgaaccagc ggcccagagg gatcagggca gacgaatacg
      961 ccgctgtgga aaccaccgcc catttttcgt ccggcacgga tgcgttgcgc attgaggggg
     1021 gcagagagca ggcgggagtg gcgtggtcgc cgaaccttgc cgtgcgggcg aatgcgtggt
     1081 ccgcgtattt tgaacccgtc gcctgccgca tcaggcagtc tggcactcat gtagcagcag
     1141 gagtctctcc cgcggctgca acgggcagcg gactcctggt gatcaccgcg caggcgagtt
     1201 catcctcgcc gctcaagggc ttgaccgtcc gcctttgcta cgaggtctat gacggtttcc
     1261 cggtcatccg gaagtgggtc gagattacca atggcgggac gcggtggctc aaactcaaca
     1321 aactggtcat tgacgacctg gaactcgctg ccgattgccg ccaccagacg ccgttgacgc
     1381 caggcgaacg cggcgctggc cccagcgtca ttgcgtttgg gacaccggac ggacggcgcg
     1441 gcgtagtcgc ggtgagcgaa attccatccg cgttgcgcga aacgagcgac acgggggcga
     1501 tgggttatag cgaggagctt tttgaatggg tgctggggcc aggggagacg ttcatctcgg
     1561 aaccggtttt cttcctggcg tacagcggtg agttgcataa gacgatctcg gccatctcta
     1621 ctccccgcga ccggacggtg gaaggcgttt acctcgactt cctccgcaaa caggtcggcg
     1681 tggctgctga caaagggacc atcgaaactc cgcaatggtg ttcgtggtca aacttcggct
     1741 ggaccctcaa tgacggcatc gtgcgccagc aagccgagct cgcggcacga tgcggttttg
     1801 cgctgttgct gctggattca ggctggcaga aagataccat cggcactgaa gctgacgcga
     1861 ccaggttccc cgacttcgcc gccacctgcc gtcacatccg ttcgctgggg ctcaagctgg
     1921 gcctttgggt gtcgtgcttt cgggtggccg attcgcccga cctgcgggcc aagcccgagc
     1981 tgcgcagcgc tccgctcatc aagcgcgatg gcggcttcgg catgagcttt gccagcccct
     2041 ggaaagagtt ctacgccaag gacctggccc gcatcagtca caagtatggc gtgagttatt
     2101 tcaaacagga ctacaccaac gtcaagctgg gcgacagcgc cgaaggtcac gaaagccgca
     2161 cgcaccggga atcgctcttg cgcgggctgc ggggattgtt ggtcgcccag gacctgctgc
     2221 gccagtccgc cccgcaggtg gctccggagg tcacccacga gatttattgg ggcacccctg
     2281 gggtgccctg cgacgtggcg gtggtcaaac atgccgtggc ctatcacatt ccgcccaacg
     2341 attatgccgg ggtgggccat cccaaggagc gggtgcgcac caattggccc tatgagccgg
     2401 ccaagctgcg ccagcagttg ctggacggct gtttcaacgc ccgccaacgc ttctatgccc
     2461 atcgcggcct gccgctctac gccctcgaat actatgccgc ccacacggtc aacttccgtg
     2521 gcagcctcac ccccgcggtc cagcaacgac aggtttgcag ttggctcatg ggcgtgccgt
     2581 ccaccttcgc tggcgacctc gcctcgctga cggaggaaaa catcgcttgt tatcgacggc
     2641 tcttcaccct cctgcagcgg cttgagaagg attacggcat ttatcgtcat ttccaattca
     2701 gcggcgcgcc ggcccccaca gatacggatt ggcactggtg gggcaagctc aacggccagg
     2761 gctgcggcgc ggtggtggtg ctgcgcggca gccagggggc cgatgaacgt gccgtgaaca
     2821 tcccgtgggt tttgcccggc tgcacttacc aggtgaacgg cttgcttgcc caccagtcgc
     2881 tgggccgttt cagcggtaaa gaacttcagc agggcgcttt ggttttaaag ctcgccccct
     2941 ttggacagga gattctcgaa tgcaaagccg agtgaacttc cgattcgtct ttttcctatg
     3001 cttcaccgcc gcgctggggg ggctgctgtt tgggttcgat attgccatta ttacgggtgc
     3061 ggggccgttt ctgctgcagc atttcaaact cgatgatctg agcctgggtt gggcgttcag
     3121 ttccctgctg ttcggctgca tcctgggttc ggttatcgcg ggtcggctga ccgacctggt
     3181 ggggcgccga cgggttttac tggccgtcgc cgggctcttt gcgctgacga cggttgccac
     3241 cgggctgtcg ccaagctttt cctggtttat cctggcgcga tttctgggcg gactggccgt
     3301 ggggggagcg tcgattgtct cgccgatgta cgtggcggag attgcccccg ccaacctgcg
     3361 cgggcgcatg tgcgcttttt accagctttc catcgtcatc ggaattctgg cttcctattg
     3421 tatcaacttc ctgctgcgcg atgcggggct ctggaactgg cgctggatgt ttattagcgg
     3481 ggtcattccc tcgatagtct tcctgctcat gctggtgcgc gcacccgaaa cgccccgtta
     3541 cctcttcaag tgcgggcgtc aggccgaggc gaccgccctg ctttgccggc tcgtcggccc
     3601 ggagcaagcg gcccgcgaga tagcggaaat ccaggccagc ctggccgaac accatgccgg
     3661 gtggcgcgaa ttgctgcacc ctggtctgag acgtgtcctg gcggtgggtt ttgtgctggc
     3721 ggtgctggtt catttttcgg gcatcaacac gatcattgat tacgctccga ttatcctcca
     3781 gtccgccggt ttgaaagtgg atgcagcact cttctcgacg ttcatcatcg gcctgaccaa
     3841 tttcctgttc acgctggtct cgttctggac cattgatcgg tttggccgca ggcccctcta
     3901 catcaccggt tcgctcggca tgaccgttgt gttgatgttg ctggcggtgg cgtcggtgct
     3961 caaccgcttc acgggcgtca tcgccctcgg cctgatcatg gcctatatcg ccttcttcgc
     4021 ctcctgcatt gggccagtgt tctggacgct ggtccccgag atctttccga atcggattcg
     4081 tggcacagcc atgtcagttc cggtgctgac gcagtgggca gccaacgcgg tggtggttct
     4141 tttctttccc ctggctttta accatatcgg caaggcccct acgtttgggt tcctgggcat
     4201 catggccttg gcccaggctc tcttcctctg gcgttttgtt cccgagacca aaaacatgac
     4261 ccttgagcag atcgaggaga tatggaaaaa cgcgggtaac cggctaacgg gagtcgataa
     4321 aaaggagcat gacaaagtgt cccgataacc gctgcg
//
LOCUS       DATCGS010000109         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_159020, whole genome shotgun sequence.
ACCESSION   DATCGS010000109 DATCGS010000000
VERSION     DATCGS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159020"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VIL39_02125"
     CDS             complement(<1..839)
                     /locus_tag="VIL39_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5480254.1"
                     /translation="MNRVLFALLALATAMTAAAVDAKIVFLAGPPSHGPGDHEHRAGC
                     LLLKSCLDNIPGVTSEVYSNGWPQNPATAFAGAATVVIYSDGGGGHPFLHGDRLKTVG
                     ELMKKGVGLVCLHYAVEPTKEAGEKEFLDWIGGCFETDWSVNPTWRPDFKPMPKHAST
                     RGVNPFTVPDEWYFHMRFRDDMKGVMPILSALAPESTMSRPDGPHEGNPAVREAVKRG
                     EPQIVAWASERLDGGRGFGFTGGHFHKNWGNDDLRKLVLNAILWTAKVEVPDMGVESK
                     VTD"
     gene            complement(902..1615)
                     /locus_tag="VIL39_02130"
     CDS             complement(902..1615)
                     /locus_tag="VIL39_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5480255.1"
                     /translation="MNSKIFSALMGAVLLAQPVVVPAAEQGFLSIFNGKDLTGWEGNP
                     KLWSVKDGAITGQTTAENPAKGNTFLIWTNGTPSDFELRCSFKLTPGDSKGFANSGIQ
                     YRSKVFDPANWVVGGYQADMEAGPSYTGILYEERMRGIMALRGEKVVWDKEDKKQVVG
                     SVGDAAAIGAAVKKGDWNEYVIIAKGNHLQQFINGKQTIDLTDDCEAKRAMSGVLALQ
                     LHAGPPMMAQFRNLRIKKL"
     gene            complement(1655..2500)
                     /locus_tag="VIL39_02135"
     CDS             complement(1655..2500)
                     /locus_tag="VIL39_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5480256.1"
                     /translation="MRFGINTFLLTSPFTNESTKLFKQIKQWGFDTIEIPIEDPSHID
                     PAHVKRELDKHGLVCGSVCACLGPDRDLRGTPQQQKNSLDYLKKVIDQMVVLDCSSVV
                     GPVYSSVGRADAVPAAEYKRQWKTVVRNLKTLAAYAQKRGKQICMEPLNRFETDFINT
                     CNQALKMVKAVGSPALKLHLDTFHMNIEEKCQAAAIRKAGKLLGHFHACGSDRGTPGN
                     DHIDWKSIAKALKDVRYQGDVVIESFTTDVKVIARAAAIWRRIEPTRNEIAVKGVKFL
                     RKTLV"
BASE COUNT          521 a          883 c          769 g          582 t
ORIGIN      
        1 tcatcggtga ccttgctctc gacgcccatg tccgggacct cgaccttggc ggtccagaga
       61 atcgcattga gcaccagctt ccggaggtca tcgttccccc agttcttgtg gaagtggccg
      121 ccggtaaacc cgaatccgcg ccctccgtcc aggcgctcac tggcccaggc gacgatctgc
      181 ggctcaccgc gcttcacagc ctcgcgcacc gccgggttgc cttcgtgcgg accgtcgggt
      241 cggctcatcg tgctttcagg cgccaacgcc gacaggatcg gcattacgcc cttcatgtca
      301 tcgcggaagc gcatgtggaa ataccactcg tcgggaaccg tgaacggatt caccccgcgc
      361 gtgctggcat gctttggcat cggcttgaaa tccgggcgcc aagtcgggtt caccgaccag
      421 tctgtctcga aacagccgcc gatccagtcc aggaactcct tctcgcccgc ctccttggtc
      481 ggttccaccg cataatgaag gcagaccagc ccgaccccct tcttcatcag ctccccgacc
      541 gtcttgaggc ggtcgccgtg gaggaagggg tggccgccgc caccgtcgct atagatgacg
      601 acggtcgccg cgccggcaaa agcagtcgcc ggattctgcg gccagccgtt gctgtaaacc
      661 tctgaggtca cgcccgggat gttatccagg caggacttga gcaagagaca gccagcgcga
      721 tgttcatggt cgcccggtcc gtggcttggc gggccggcga ggaaaacaat ctttgcatcc
      781 acggcggcgg cagtcatcgc cgtcgccaat gccagaagtg cgaaaagaac tcgattcatt
      841 gttttagata ggacttgggc tgaaacgaat caaccgcgca cacgggcgca ggcgggcacg
      901 cctacagctt tttgatccgt agattcctga actgcgccat catgggcggg ccggcatgga
      961 gctgcagcgc caacacgccg ctcatggccc gcttggcttc gcagtcatcc gtcagatcta
     1021 tggtctgctt gccgttgatg aactgctgca ggtgattgcc tttggcaatg atcacgtact
     1081 cgttccagtc ccccttctta accgcggctc caatagcggc ggcatccccg accgagccga
     1141 cgacctgttt cttgtcctcc ttgtcccaga cgaccttctc gccgcgcagc gccatgatgc
     1201 cgcgcatacg ttcctcgtag agaatgcccg tgtagctcgg ccccgcctcc atgtccgcct
     1261 ggtagccgcc caccacccaa ttcgccgggt caaaaacctt gctgcggtac tggataccgg
     1321 agttggcaaa gcccttactg tcacccggcg tgagtttgaa ggagcagcgc agctcgaagt
     1381 cgcttggggt gccgttagtc cagatcagga acgtgtttcc cttggctggg ttctcggcgg
     1441 tggtctggcc ggtgatggcg ccgtctttga cggaccatag tttggggttg ccctcccagc
     1501 cagtcaggtc tttgccgtta aaaatactga ggaagccctg ttccgcagcc ggtacgacca
     1561 ctggttgggc caatagaact gcccccatga gggcgctgaa tattttgctg ttcataatct
     1621 tcttttattc gcgaacgata cgcctgtcct gctatcacac aagtgtcttg cgcaagaact
     1681 tcacgccctt caccgcaatc tcgttgcgcg tcggttcaat gcgccgccag atggccgccg
     1741 cgcgggcaat caccttcacg tccgtcgtga aggactcgat caccacgtca ccctggtagc
     1801 gaacgtcctt cagcgctttg gcgatggact tccaatcaat gtggtcgttg ccaggcgtgc
     1861 cgcggtcgct gccgcaggcg tggaaatggc cgagcagctt gcccgccttg cgaatcgcgg
     1921 cggcctggca cttctcctca atattcatgt ggaacgtgtc gaggtggagc ttcagcgccg
     1981 ggctgccgac cgccttcacc atcttcagcg cctggttgca ggtgttaatg aagtcggtct
     2041 caaagcggtt gagcggctcc atgcaaatct gtttgccccg tttctgcgca tacgcggcga
     2101 gcgtcttgag attcctcacc accgtcttcc actgcctctt gtattcggcc gccggcaccg
     2161 cgtcagcccg gcccaccgac gaataaaccg gcccgaccac cgacgaacaa tccagcacga
     2221 ccatctggtc aataaccttc ttcaggtagt cgaggctgtt cttctgctgc tgcggcgtgc
     2281 cgcgcaaatc gcgatccggt cccaggcagg cacagaccga gccgcagacc aggccatgct
     2341 tatccagctc gcgcttcacg tgcgccgggt caatatgcga ggggtcctcg atgggaatct
     2401 caatcgtgtc aaagccccac tgcttgattt gcttaaacag cttggtgctt tcgttggtaa
     2461 acggtgaggt gagaagaaag gtatttatgc caaatctcat aattgagacg gaagctaatt
     2521 catcaaacga ccgtgtcaac ccctttccag ccatttctgc cgtgctgaga gcatcccatt
     2581 tcctgaacag cctatgggaa ccgagtggaa tgaacaaagg cgaagcccgg tagtccaaga
     2641 aaaggctgcg gtgccagagc agcatcgaaa ccccgcccag cgccccggtt tcgagccaac
     2701 tcatcacgtc gcccagaccg tccagagacc gtaacgggcg gctgatttcc cttga
//
LOCUS       DATCGS010000110         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_703450, whole genome shotgun sequence.
ACCESSION   DATCGS010000110 DATCGS010000000
VERSION     DATCGS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703450"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..458
                     /locus_tag="VIL39_02140"
                     /pseudo
     CDS             1..458
                     /locus_tag="VIL39_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001977158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
     gene            complement(577..1845)
                     /locus_tag="VIL39_02145"
     CDS             complement(577..1845)
                     /locus_tag="VIL39_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY5480257.1"
                     /translation="MPPEQVETASVTATAHRASFFRQSGWLMIATVAGGLFMTAMHFL
                     SKVIPKSEYSQFGVFLSVAMFIPAGPLQMVLAQQTAQARALHREHELSGLIRAAWLGT
                     FVIWLVGAGLVLLFQDTIMAQWKITNPAAIWLTLPVLLFTVWLPMFSGVLQGQQNFLW
                     LGWSGIANGVGRLTIAACAVLVLHYFASGMVLGMLAGLAVALVIAIWPTRSLWLPSPQ
                     AFEWRSLLRQIVPLTLGFGAYQFLFTADTMFVKAYFTGDEAAYYVSAGTLARASLWLV
                     GPLAAVMFPKIVHAKAKAEKSDLMGIVLIGTVILAAGGATGLWVLGPWVVRFMFEPSY
                     VRDASVLLPWYAWAVVPLSVANVLLNNLLARSLFKVVPALCVLVGVYVFALTQFHATP
                     VMVIKTLGTCNVLLLAVCAYYTWGVRQSKV"
     gene            2176..4017
                     /gene="rpoD"
                     /locus_tag="VIL39_02150"
     CDS             2176..4017
                     /gene="rpoD"
                     /locus_tag="VIL39_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5480258.1"
                     /translation="MRKTKPRKAIQEIGKGVRAVTPEAVLSILPVRRRRGRPPRVPQE
                     PLFSPTASLAKARQPVGSISGQASLDLTQKVKDLVCLAKEQGHLTHDDVNDALPDEVV
                     TPADLDHVLSKLRGLEIEIIDAAEVERGRRAEADGEDGENESGRYDILDDPVQMYLRQ
                     MGKVPLLTREQEVEICKRTEAAEEAACRIIYGFGFAAKEHLALADKLLAVPPKERFDR
                     VIVDKFIGSRPRHLKFLRRLVKRVRALDRQADEKFSGWQQAREKTQRARLLYQFMRFD
                     RKLKAAYPRFHYKQKVIDDMTLVAENIRDKIQAGLRAVEALEKQCKSTAQQAIIQSEK
                     DKLRTLERFVRLPQADYLKACAELNGAMARAQEAKTQMVEANLRLVISIAKKYTNRGQ
                     SFLDLIQEGNVGLMKGVEKFEYRRGYKFSTYATWWIRQAITRCIADQARTIRIPVHMI
                     DIINKLCRAQKQLMQELGREATTEELGDSLEMPVERVRAVLKLAQQPVSLQSQMGDTE
                     DAHFGDLIEDKTAENPSEVTSFHLLCGKLGEVLRGLTERERQILELRYGLIDGSPRTL
                     EEVGRQYEVTRERIRQIEAKALRKLRHPTRKNRLEGFLETTTVPLVA"
     gene            4025..4975
                     /locus_tag="VIL39_02155"
     CDS             4025..4975
                     /locus_tag="VIL39_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5480259.1"
                     /translation="MNIVVLDGFTLNPGDLSWEQLRSLGPCAIYDRTLPAELLGRAAD
                     ADVLLTNKTELTADTLQSLPRLKYIGVLATGTNVVDFTAARARGIPVTNVPAYSTKSV
                     AQLTFALLLELAQHAGHHAQTVREGRWTRSTDFCYWDFPLIELDGLTLGIVGFGRIGR
                     AVGELGSAFGMKVLACARTAGSAPPFASFVELETLFRKSDVVSLHCPLTPQTANLVNA
                     RRLSLMKPTAFLLNTSRGPLVDEPALADALNSGRIAGAALDVLSKEPPRADNPLLTAR
                     NCLITPHLAWATRAARSRLMTIAVENVRAFLQGKAQNVVN"
     gene            5063..5257
                     /locus_tag="VIL39_02160"
     CDS             5063..5257
                     /locus_tag="VIL39_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480260.1"
                     /translation="MTYWIRMMVSGWRRLFKPRQTTADRSTLVGMYLSHANRPLDSSA
                     RGSETRERQEGKFSQNRRRG"
BASE COUNT         1094 a         1713 c         1587 g          909 t
ORIGIN      
        1 ggttcccggt taccgtggac cacatgagcc aactggccga tcagcttggg gaaaagacgt
       61 tgttcgggcg cacgggcggg gcggcgacgt tggcggtcgg catggcgcac attttcgcca
      121 aagccgtgca cgggcgctgg ctcgacctgt ggtatcactt cgccattatg ttcgaggcgc
      181 tgttcatcct gaccaccatt gatgccggca cacgcgtggg gcgctacgtg ctccaggatg
      241 tgctgggcaa cctctggaag ccgctgggcg aaacgaagag cctgggcgcc aatctgctgg
      301 cgagcatgct gctggtcagc ggctggggct atttcctgat tcaaggcacg cgcgatccgc
      361 tcgggggcgt gaactcgctg tggccgctgt tcggcatcgc caaccagatg ctggcggcga
      421 tcgggctttg cctcggaacc acgattatcc tgaagatgac gctgcttgat cagtctaaag
      481 gccaaagccc aaagtccaaa gtccaaggtc taaagtccaa agtccaaagt cagggaacgg
      541 cttactcccc agactttgga cattggacat tggacattag actttggact gtctgactcc
      601 ccaagtgtaa taggcgcaca cagccagcag cagcacattg caggtaccga gcgtcttgat
      661 gaccattact ggggttgcgt gaaactgggt gagcgcaaag acgtaaactc caaccaatac
      721 gcagagcgcc ggcaccactt tgaagagcga gcgggccagc aggttattca gcagcacgtt
      781 ggccaccgac agcggcacca ccgcccacgc ataccacggc agcaggacag aagcgtcccg
      841 cacatagctg ggctcgaaca tgaacctcac cacccacgga cccaacaccc acaaaccggt
      901 cgccccgcct gcggccagga tgacggtccc gatcagcact atccccatca ggtcgctctt
      961 ctccgccttc gctttggcgt gaacgatctt ggggaacatc accgccgcca gtgggccgac
     1021 cagccacagc gaagcgcgag ccagcgtccc ggcgctgacg tagtacgcgg cctcatcgcc
     1081 ggtgaaatag gccttcacga acatcgtgtc cgcagtgaac aggaactgat acgccccgaa
     1141 ccccagcgtc aaggggacga tctgacggag caggctgcgc cactcgaaag cctgcggcga
     1201 cggaagccag agcgaccgcg tcggccagat cgcgataacg agggcgaccg ccaggccggc
     1261 cagcatcccc agcaccatgc ccgaggcaaa gtaatgcagc accaggacgg cgcaggcggc
     1321 tatcgttaac cggccgactc cgttggcgat tccgctccag cccagccaga gaaagttctg
     1381 ctgcccttgc agcacgccgg agaacatcgg caaccagact gtgaacagca gcaccggcag
     1441 cgtcagccag atcgcggcgg ggttggtaat cttccactgc gccatgatcg tgtcctggaa
     1501 cagcagcacc aggcctgccc ccaccagcca tatcacgaag gtccccagcc acgcggcccg
     1561 gatcaagccg gacagctcgt gttcccggtg gagcgcgcgc gcctgggcgg tctgttgggc
     1621 caaaaccatt tgcagcggcc cggctgggat gaacatggcc accgaaagga acaccccgaa
     1681 ctgcgaatac tccgattttg ggatgacctt ggacaggaaa tgcatcgccg tcatgaacag
     1741 cccgcccgcc acggtggcga tcatgagcca gccgctctgg cggaagaatg acgcgcgatg
     1801 cgccgtagcg gtcacggaag cggtttcaac ttgttcaggc ggcatgagaa ttggagggga
     1861 ctaggggtgg ttccgttcaa gaagatgact ccaggaagcg ccgcggccga acagtcgtcc
     1921 gttccgcttg ctcggcagcg cgatgagttc gcgcaatagc ttctcagttt tcgtcatgcg
     1981 gttcccgata ggggattgag gggcataacc tacccagggc ggcgccggct gtcgattctt
     2041 tcgtcaacac tcgatgattt tatgtgcttg cgccggcact cgcgaggtcg tttgatttca
     2101 ggaagcttga acggttgcct ctgaggcggc acgctcgttt tctgggcgaa cgaatccagt
     2161 gggcagtgcg acaatatgcg gaagacgaag cctagaaagg caattcaaga gatcggcaaa
     2221 ggggttcgtg ccgtcacccc cgaggccgtc ctctctattc tgcccgtccg ccgtcgccgc
     2281 ggccgtccac ctcgggtccc gcaggagccg cttttctcgc cgactgcttc attggccaag
     2341 gcccggcagc cagtcggttc gatctctggc caagccagtc tggatctgac ccagaaagtc
     2401 aaagacctcg tatgcctcgc caaggagcag ggtcacctga cccatgacga tgtcaacgac
     2461 gcgttgcccg acgaggtggt cactcccgcg gatctggacc atgttctcag caagctgcgt
     2521 gggctggaga tcgagatcat tgatgccgca gaagttgagc ggggccggcg cgccgaggcg
     2581 gacggagagg acggagagaa cgaatccggc cgctacgata ttctggatga tccggtgcag
     2641 atgtatctgc gccagatggg caaggtgcca ttgctcacgc gcgaacaaga ggtggagatt
     2701 tgcaagcgca ccgaggccgc tgaagaggcg gcgtgccgga tcatctatgg ctttggtttt
     2761 gccgccaaag agcacctggc gctcgcagac aagctgttgg ccgtgccgcc caaggaacgg
     2821 tttgatcgag tcatcgttga caagttcatc ggcagccgcc cccgccatct caagttcttg
     2881 cgccggctgg tcaaacgcgt ccgtgccctg gaccggcagg ccgacgagaa gttcagcggc
     2941 tggcagcagg cgcgcgagaa gacccagcgc gcgcgcctgc tctaccagtt catgcggttt
     3001 gaccggaagc tcaaggccgc ctacccgcgc tttcattata agcagaaggt cattgatgat
     3061 atgacgctgg tggccgagaa catccgcgac aagatccagg ccggcttgcg cgccgtcgag
     3121 gccttggaaa agcaatgcaa atccaccgcc cagcaggcga ttatccagtc tgaaaaagac
     3181 aagctccgca ccctcgagcg ctttgtgcgc ctgccgcagg cggattacct caaggcttgc
     3241 gccgagctaa acggcgcgat ggcccgcgcg caggaagcca agacccaaat ggtcgaggcc
     3301 aacctgcgtc tcgtcatctc catcgccaag aaatacacca atcgcggcca gtccttcctc
     3361 gacctgattc aggaaggcaa cgtcgggctg atgaaaggcg tcgagaagtt cgagtatcgg
     3421 cgcggctata agttttccac ctacgccacc tggtggatac gccaggccat cacccgctgc
     3481 atcgccgacc aggcgcgcac gatccgcatc ccggtccaca tgattgacat catcaacaag
     3541 ctctgccgcg cgcaaaagca gcttatgcag gaattggggc gcgaggccac taccgaagag
     3601 ctgggcgaca gcttggaaat gcccgtcgaa cgcgtgcgcg ccgtcttgaa actggcgcag
     3661 cagcctgtct ccctgcaatc gcagatgggt gacaccgagg atgcgcattt cggcgacctc
     3721 atcgaagaca agactgccga gaacccgagc gaggtcacca gcttccatct gctctgcggc
     3781 aagctgggcg aagtgctccg cggcctgacg gagcgcgagc gccagatcct ggaactccgt
     3841 tacggcctga tcgacggctc cccgcgcacg ctggaggaag tcggccggca atacgaggtc
     3901 acgcgcgagc gcattcgcca gatcgaggcc aaagccctgc gcaaactccg ccacccgacc
     3961 cgcaagaaca gactcgaggg attcctcgaa accacaactg tgcccctggt cgcctgacac
     4021 ggtcatgaac atcgttgtcc tcgatggatt caccttgaat cccggcgacc tgagctggga
     4081 acaactccgg tccctcggcc cctgcgccat ttacgaccgc accctgccgg ccgaactcct
     4141 gggccgcgct gccgacgccg acgtcctctt gaccaacaag acggagctga ccgcagacac
     4201 cctccagagt ctgccccggt tgaaatacat cggcgtgctg gccaccggaa ccaatgtcgt
     4261 ggacttcact gccgcccgcg cccgcggcat accagtgacc aatgtgccgg cttacagcac
     4321 gaaatccgtg gcgcaactga cctttgccct gctcctcgaa ctcgcgcagc atgccgggca
     4381 tcacgcccaa accgttcgcg aaggccgctg gacgcgcagc acagattttt gttactggga
     4441 cttcccgctg atcgagttgg atggactcac cctcggcatc gtgggttttg gccgcattgg
     4501 ccgggcggtg ggggaactgg gctcagcgtt cggcatgaag gtgctggcct gcgctcgcac
     4561 agcggggagc gctccgccct tcgccagctt cgtcgagctt gaaacgctct tccgcaaaag
     4621 cgatgtcgtc agcctgcact gtccgctcac gccgcaaacc gccaacctgg ttaatgcccg
     4681 gcgcctgtcg ctgatgaagc cgactgcctt cttgctgaac accagccgcg gccccctcgt
     4741 ggacgagccc gccctggccg acgccttgaa ctccggccgc atcgccggcg ccgccctgga
     4801 cgtcctatcc aaggaacccc cgcgcgcgga caatccgctg ctcaccgcca ggaattgcct
     4861 tatcaccccg cacctggcct gggctacccg cgcggcccgc tcgcgcctga tgaccatcgc
     4921 cgtcgaaaac gtccgcgcct ttctgcaagg caaggcgcag aatgtggtga attgatccag
     4981 cgtagcgcag cccgagggca ttactaattt gccgaaagcg cagttgcatc gctcaaccga
     5041 ttccagttta atcccgccgc gcatgaccta ctggatacgc atgatggtga gcgggtggag
     5101 acggctcttc aaaccgcggc agacgacggc ggaccgctcc acgctggtgg gaatgtactt
     5161 gtcgcacgcc aaccggccct tggactccag cgcgcgcggc tcggaaaccc gggaacggca
     5221 ggaaggcaag ttctctcaga accggcgtcg cggatgagaa ccgccccgcc ctgcgtccga
     5281 tcaggaagac ccccaggact ttg
//
LOCUS       DATCGS010000111         7784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_159324, whole genome shotgun sequence.
ACCESSION   DATCGS010000111 DATCGS010000000
VERSION     DATCGS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159324"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1275)
                     /locus_tag="VIL39_02165"
     CDS             complement(121..1275)
                     /locus_tag="VIL39_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY5480261.1"
                     /translation="MRFEFATATRVIFGPGVLREVGPLARRFAPRALVVAGANPRRAE
                     PLLSLLREYGVAAITFPIPGEPEIETVQQGVALAKQENCGVVISFGGGSALDAGKAIA
                     AMLSNTGELLDYLEVIGRGKALAEPSAPFIAIPTTAGTGSEVTRNAVLASRAQGVKVS
                     LRSPFMLPRVAVVDPELTYDLPPAVTASTGLDALTQLIEPYVCSRANPMTDALCVEGI
                     RRAARSLRVAFADGRNAAARTDMAVASLFGGLALANAGLGAAHGFAGPIGGQFPAPHG
                     AVCAALLPQVMEANLRALRQRQPAAETLRRYEEVARLLTGSATTTAEPGVEWVRKLVA
                     DLGIPSLGHYGLKPEHMAELVEKASQASSMKANPIVLTRDELAGILQRAL"
     gene            complement(1291..1596)
                     /locus_tag="VIL39_02170"
     CDS             complement(1291..1596)
                     /locus_tag="VIL39_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5480262.1"
                     /translation="MLVVHVHVHVKPEYVEVFKQATIVNAQASSKEPGIARFDFAQQQ
                     DDPTRFVLVEAYRTAEAPAAHKETRHYQAWRNTVAPMMAEPRTSVKFGNMFPDDANW"
     gene            complement(1704..2201)
                     /gene="msrB"
                     /locus_tag="VIL39_02175"
     CDS             complement(1704..2201)
                     /gene="msrB"
                     /locus_tag="VIL39_02175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY5480263.1"
                     /translation="MSTFQKPPVAELKQKLTPMQFEVTQHAATEPSFRNEFWDSHRPG
                     IYVDIVSGKPLFSSLDKFDSGCGWPSFSRPVAANEVVEHTDNSLGMKRTEVRSSAADS
                     HLGHVFNDGPGPTGLRYCINSASLRFIPVEQMEATGYSAYLEPFVKAGLYKPKDAKGA
                     EAPKR"
     gene            2203..2394
                     /locus_tag="VIL39_02180"
     CDS             2203..2394
                     /locus_tag="VIL39_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480264.1"
                     /translation="MELVNGGGAAAEVFVEVCDAARAQPARTTTAKAAAALGQLKGFT
                     GFMQDNSTREAAAKIKRQQ"
     gene            2431..2817
                     /locus_tag="VIL39_02185"
     CDS             2431..2817
                     /locus_tag="VIL39_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY5480265.1"
                     /translation="MTEPNNQQPAIRVVMMPRDTNPMGTIFGGIILSYIDQAGAVEFR
                     KQSDKRMVTVAMHEVKFLAPVFVGDLVSFYTETVKIGNTSLTARVTVEAKRGQAPHES
                     VRVTQAEVVYVALDGQGKPVPVRQTG"
     gene            complement(2818..4251)
                     /locus_tag="VIL39_02190"
     CDS             complement(2818..4251)
                     /locus_tag="VIL39_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480266.1"
                     /translation="MKSALNRRSFLKRSALAAGALSAGGLLPVPYALGAARPGAKLNC
                     VQIGCGGRGMSHLDWVVTQSKDNLVALVDADEKNHAKVKKWLEKREVDPSKLQVFTDY
                     RVMFDKIGKQIDAVFIATPNHHHALPAMIAMQLGKAVYCEKPVCHDIAEARKLRAMAS
                     QSKAPTQMGNQGHCEDGYRRLCEFVWAGVVGNIKETHSWTDRANGGAGPRPPSLPAPA
                     GLHWDNWIGPAPYRDYHKGLHQHDWHDWYDFGNGSIGNMGCHVLDGVFWALKIEHPTS
                     VEAEEIRGGSDERYPTGSRVRWDIPARGDMPPMKAYWYEGFNQDAKGNPADDPKAAKG
                     KDRNFPPLLAELQKQYPDEEMDRPDSGTIYVGEKGVLYTGTYGGRMHILPMEKMQQIT
                     QPPRTLPRPKNIMADFLNACREGKTETAASFDYGTRLTEFTLLGNLAQHAGVGKKVEW
                     DGPNMKVTNLPELNQWLKRPYRKGWTA"
     gene            complement(4391..5728)
                     /locus_tag="VIL39_02195"
     CDS             complement(4391..5728)
                     /locus_tag="VIL39_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5480267.1"
                     /translation="MNIIQLTPGAGAMYCGGCLRDNALVAALRKLGHQALMVPLYLPL
                     TLDEADQSAGTPVFFGGISVYLEQKTALFRGAPGWLHDLFASRRLLAWAAGKAAKTRA
                     ADLGELTLSMLRGEEGNQAREIEVLIAWLKTQPKPDVLSLSNALLIGIVRRLKAELRI
                     PVVCALQGEDSFLDALPESHRAACWRTLAERAAEVDLFVAPSRYFGDLMRERLGLPAD
                     RVRVVHNGINLEGYAVEGQTEKSEVQSPKSQVQGTGVPVLGFFARMCREKGLDTLVEA
                     YLLLRQRGRVGQLKLRVGGSCGPADGAFVDSLRERLQAGGVLEDVEFHPNADRASKVA
                     MLRSLSVFSVPALYGEAFGLYVIEALAAGVPVVQPRVAAFPELLEATGGGVLCAAGDA
                     RALAEAIEELLLNPARARALGEAGRRAVWERFSAEAMARETLQVLGDVAGRRR"
     gene            complement(5725..6927)
                     /locus_tag="VIL39_02200"
     CDS             complement(5725..6927)
                     /locus_tag="VIL39_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5480268.1"
                     /translation="MNLARHIVLWSWLLVSWCASAAAPGDDWPMFRGGPALAGVAGGS
                     LPEKLDLLWTFKTAGPVKSSAAIVQDRVFIGSDDGNVYALALADGKKVWAFKTGGGVE
                     SSPLVLEGKVFVGSSDGWLYALEAGTGKLAWKYETGDKILGAPNWVKVNAGSGASVSP
                     AAPTASAAVEGAPGGRRDARPTFVLVGSYDFKLYCVDAGTGRSNWVYETGNYINGSPA
                     VANGQAAFGGCDGLLHVISLAEGKQVKEVEAGAYIAGSAAMADGCAYFGQYENEFLCV
                     DLKAGKKAWAYHDRNFPYFSSPAVTQETVVFGGRDKLLHCVKRADGQAVWSFATRGKV
                     DSSPVVCGNKVVVGSDDGRLYVVSLDKGKELWSYEIGQPIESSPAVARGKVVVGAGDG
                     NVYCFGGK"
     gene            complement(7303..7527)
                     /locus_tag="VIL39_02205"
     CDS             complement(7303..7527)
                     /locus_tag="VIL39_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480269.1"
                     /translation="MAKDAPVVQRLARSRKVAYLTHWLFLPHSEKKALRWIDFTANNF
                     ARRSSVEFAIQLKHGGRMVGLVGWDPNLEP"
     gene            7526..7771
                     /locus_tag="VIL39_02210"
     CDS             7526..7771
                     /locus_tag="VIL39_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480270.1"
                     /translation="MRGRNSRELVRAMDAEKWFGLMPEAVGAAKAARESGAAGKIVAL
                     PKVPGQRARGSGAVDRGNTVWSGTRTAASEGDERISL"
BASE COUNT         1423 a         2512 c         2404 g         1445 t
ORIGIN      
        1 cacctcaaat tcggcttcaa atcctggtga cattacccga ggacgggcgc actccgatca
       61 gcgcggcgtg ccctcacgcg gcgatccttg tatcggttac ttgagcaacc aagcaccagt
      121 tcaaagcgcg cgttgcagga ttccggccag ctcgtcgcgc gttagaacaa tggggttcgc
      181 cttcatgctg ctggcctggg aggctttctc gaccaattcg gccatgtgtt ccggcttaag
      241 tccgtaatgg cccaggctcg ggatgcccag gtccgcgacc agcttgcgca cccactcgac
      301 gccgggttcg gctgtcgtcg tcgcgctgcc ggtcagcagg cgtgccacct cttcgtagcg
      361 ccgcagtgtt tcagccgcgg gctggcgttg gcggagggcg cgcaggttcg cttccatgac
      421 ctggggcagc agcgcggcgc agacggcgcc gtggggtgcg gggaattggc cgccaatcgg
      481 cccggcaaac ccgtgcgcgg cgccaagacc cgcattcgcc agggcgagcc cgccaaacaa
      541 gctggccacg gccatatccg tccgggcggc ggcgttgcgg ccgtcggcga aggctacgcg
      601 caatgaacgc gcggcgcggc gaatgccctc cacgcacaac gcatcggtca tcgggttggc
      661 gcgggagcaa acgtaaggtt cgatgagctg cgtgagcgca tcaagcccgg tgctggcggt
      721 aacggcgggc ggcaagtcgt aagtcaactc cgggtcaacg accgccactc gcgggagcat
      781 gaacggactg cgcaggctca ctttgacccc ctgcgcccgc gaggcgagca ccgcgttgcg
      841 ggtgacctcg gagccggtgc ccgcagtcgt cgggatcgca ataaaaggcg cggaaggttc
      901 ggccagcgct ttgccgcggc caatcacttc gaggtagtcg agcagctcgc ccgtattgga
      961 gagcattgcg gcgatggctt tgcccgcgtc cagcgcgctg ccgccgccga agctgatgac
     1021 cacgccgcag ttctcctgct tggccagcgc gaccccttgc tgcacagtct cgatttccgg
     1081 ctcgccggga atcgggaagg taatggcggc gaccccatac tcgcgcagaa gcgagagcaa
     1141 tggttcagcc cgtcgggggt tggcgcccgc gaccacgagg gcccggggtg caaaccgtcg
     1201 ggcgagcggg cctacctcgc gcaggacgcc cgggccgaag atcacccgag tcgctgtggc
     1261 aaattcaaat cgcataagac gggccgcgat tcaccagttc gcgtcatccg ggaacatgtt
     1321 cccgaatttc acgctggtgc gcggctcggc catcatgggt gcgactgtgt tccgccaggc
     1381 ttggtaatgc ctggtttcct tgtgggcagc cggcgcctcg gcggtgcgat aggcttccac
     1441 gagcacaaag cgcgtcggat catcctgctg ctgggcgaaa tcaaagcggg caatgcccgg
     1501 ctccttgctg ctcgcctgcg cattgacgat cgtggcctgt ttgaagacct cgacatactc
     1561 cggcttcaca tgaacatgga cgtgaacaac tagcatagtg ttgaattgtt ggagcgctaa
     1621 gacgtcgaag caactccagc gagagaatgt cccaagccag cggctgtttc aacctttatt
     1681 gcccggatca ctcttcaccc ggatcagcgc ttcggcgctt cagccccttt ggcgtctttg
     1741 ggtttataca gccccgcctt gacgaacggc tcgagatagg cgctgtagcc cgtcgcctcc
     1801 atctgctcga cggggatgaa gcgcagcgag gcggagttga tgcagtagcg cagccccgtg
     1861 ggtccgggtc cgtcgttgaa gacgtggccg aggtgcgaat ccgcggcgct ggagcgcacc
     1921 tccgtccgct tcatgccaag ggaattgtcg gtgtgctcga cgacttcatt ggccgccacc
     1981 ggccgcgaga aacttggcca gccgcagccc gaatcaaact tgtccagcga actgaacaac
     2041 ggcttgccgg agacgatatc cacatagatg ccgggcctat ggctgtccca gaactcgttt
     2101 cggaaggacg gctcggtggc ggcatgttgc gtaacctcaa actgcatcgg cgtcagcttc
     2161 tgctttagct ctgcgaccgg gggcttttga aatgtgctca tagtggaatt ggtaaatggg
     2221 ggcggagcgg cagccgaagt gttcgtggag gtctgcgacg cagcccgcgc gcagcccgcc
     2281 aggacaacga ctgccaaggc ggctgccgcg ctcggccagc tcaagggttt caccgggttc
     2341 atgcaggata atagcacaag ggaggctgcg gccaaaatca agcggcagca atagctggcc
     2401 agcgctgatc tccggaggca taatgggctg atgaccgagc caaacaacca gcaacccgcc
     2461 attcgggtgg tgatgatgcc gcgcgacacc aacccgatgg gcaccatttt tggcggcatc
     2521 atactgagct acattgacca ggcgggcgcg gtcgaattcc gcaagcaatc ggacaaaagg
     2581 atggtcaccg tcgccatgca cgaggtcaaa ttcctcgcgc ccgttttcgt gggcgacctg
     2641 gtgagcttct ataccgagac ggtcaagatc ggtaatacat ctctcaccgc gcgcgtgacg
     2701 gtggaggcca agcgcgggca ggcgccgcat gagagcgtgc gcgtgaccca ggctgaagtt
     2761 gtgtacgtgg ccctcgatgg gcagggcaag ccggtgccag tccgtcagac tgggtaatca
     2821 ggccgtccag cctttgcgat acgggcgctt cagccactgg ttgagttcgg ggaggttggt
     2881 gaccttcata ttgggcccgt cccattccac ctttttacca acgccggcat gttgggccag
     2941 gttgccgagc agggtgaatt cggtcaaacg ggtgccatag tcgaagctgg cggctgtctc
     3001 ggtcttgcct tcccggcacg cattgaggaa atcagccatg atgttctttg ggcgcggcag
     3061 ggtgcggggc ggttgggtga tttgctgcat tttctccatc gggaggatgt gcattctgcc
     3121 gccataggtg cccgtgtaga ggaccccctt ctcgccgacg taaatcgtgc cgctgtcggg
     3181 ccggtccatt tcctcgtcgg gatactgctt ttgcaactcc gccagcagcg ggggaaagtt
     3241 tcggtccttg cccttggcgg ctttggggtc gtcggcgggg ttgcctttcg cgtcctggtt
     3301 gaaaccttca taccaataag ccttcatcgg cggcatgtcg ccgcgcgcgg ggatgtccca
     3361 gcgaacgcgg ctgccggttg ggtagcgctc gtcgctgccg ccgcgtattt cctcggcttc
     3421 gacgctggtt gggtgctcga tcttaagcgc ccagaatacg ccgtcgagca catggcagcc
     3481 catgttgccg attgagccgt tgccgaagtc ataccagtcg tgccaatcgt gctggtgcaa
     3541 acccttgtgg taatcgcggt aaggcgcggg gccaatccag ttgtcccaat gcaggcccgc
     3601 gggcgcgggc aatgagggcg ggcgcggccc ggcgccgcca ttagcgcggt cggtccagct
     3661 atgggtctct ttaatgttgc cgacgacacc ggcccaaacg aattcacaga gccggcggta
     3721 gccgtcctcg caatggccct ggttgcccat ttgggtgggg gccttggact ggctggccat
     3781 agcgcgcagt ttgcgggcct cggcaatgtc gtggcagacc ggcttctcgc aatacacggc
     3841 tttgcccagt tgcatcgcga tcatggcggg cagcgcgtga tgatggttgg gtgtggcaat
     3901 gaagaccgcg tcaatctgct tgccgatctt atcgaacatc acgcggtaat cggtgaagac
     3961 ctgcagtttg gacgggtcca cctctctctt ttccagccac ttctttacct tggcgtggtt
     4021 cttctcgtct gcatccacga gggccaccag gttgtccttg ctttgggtga ccacccagtc
     4081 gaggtggctc atgccgcgtc caccgcagcc gatctgaacg cagttcagtt tggcgcccgg
     4141 gcgcgctgcg ccgagagcat aggggacagg caggagacct ccagcggaca aagccccggc
     4201 ggcgagcgcg gagcgcttga gaaacgagcg acgattcaga gcggacttca tagtttaaag
     4261 taacggtatt tccaggccca atgcctgcgg atgagccgca gtgtcgcacg gcctgtcaaa
     4321 agctcaagac gggtgttgcg cccggaaggt aacaaggaaa acgcgcctaa tgcttaactg
     4381 atacaacgct ttaacgcctt ctgcccgcca cgtcgccaag aacttgcagc gtctcccgcg
     4441 ccatcgcttc cgcgctgaac ctttcccaca ccgcccgccg ccccgcttcg cctagcgctc
     4501 gcgcccgcgc ggggttcagg agcaattcct cgatcgcctc cgccagtgcc cgcgcgtcac
     4561 ccgccgcgca caacacccct ccgccggtcg cttccagcaa ttccgggaac gccgcaaccc
     4621 ggggctgcac caccggcacg cccgcggcca gcgcctcaat cacatacagg ccgaaggctt
     4681 cgccataaag tgccggcacc gagaataccg acagtgagcg cagcatcgcc accttgctcg
     4741 cacggtcggc gttgggatgg aattcgacat cctccagcac cccaccagct tgcaaccgct
     4801 cgcgcaatga gtccacgaag gccccatccg ccggcccaca actgccgcct acgcgcaact
     4861 tgagctgccc cacgcgaccg cgctgccgca agaggaggta ggcttcgacc aacgtatcca
     4921 gccccttctc ccggcacatc cgcgcaaaga aacccagcac gggaacgccc gtgccctgga
     4981 cttgggactt cggactttgg acttcggact tctctgtttg cccctcgacc gcatagcctt
     5041 ccaggttgat tccattgtgg accacccgca cgcgatccgc cggcaagccg agccgctcgc
     5101 gcatcagatc gccgaagtat ctgctcggcg caacgaacag gtccacttcc gctgcccgtt
     5161 cggccagtgt tcgccagcag gctgcgcgat ggctttcggg cagagcgtcg agaaaagagt
     5221 cctcaccctg cagggcgcac accactggga tgcgcaactc ggccttgagc cggcgcacga
     5281 tgccgatcag cagcgcgttg gacaaggaaa gcacgtccgg cttcggctgg gtcttaagcc
     5341 acgcaatgag gacctcgatc tcccgcgcct ggtttccctc ctcgccgcgc aacatggaaa
     5401 gcgtcaactc ccccaggtcc gcggcgcggg ttttggccgc cttgcccgcc gcccaggcca
     5461 gcaggcggcg cgaggcgaac aaatcgtgca gccagcccgg cgccccccgg aacagggcgg
     5521 tcttctgttc gagataaaca ctgatgccac cgaagaagac cggggtgccg gcgctttggt
     5581 ctgcttcgtc cagcgtcagc ggcaggtaga gcggcaccat cagggcctga tggcccaact
     5641 tgcgcaacgc agcgaccagc gcattatcgc ggaggcatcc cccgcaatac atcgcgccgg
     5701 cgcccggagt gagttggatg atgttcactt cccgccaaaa caatacacat tgccatcccc
     5761 cgccccgacc accactttgc cccgcgccac cgccggggaa ctctcgatcg gctgcccgat
     5821 ttcatacgac caaagctcct tgcccttatc cagcgacacc acgtagagcc gcccgtcatc
     5881 ggacccgacg accaccttgt tcccgcacac caccggggaa ctgtccacct tgccacgggt
     5941 agcgaaactc cacaccgcct ggccatccgc gcgcttcacg cagtgcaaca gcttgtcccg
     6001 gccaccgaag acgaccgtct cctgggtcac ggccggggaa gagaagtaag ggaagttccg
     6061 gtcgtgataa gcccacgcct tctttccggc cttcaaatcc acgcacagga attcgttttc
     6121 atactggccg aagtaggcgc acccatccgc catcgccgcc gagccggcaa tataagcccc
     6181 ggcctcgacc tccttcacct gcttgccctc cgccagcgag atcacgtgca gcagcccgtc
     6241 gcaccctccg aaggcggctt ggccgttcgc caccgcgggc gacccgttga tgtaattgcc
     6301 ggtttcatag acccagttgc tgcggcccgt cccggcgtcc acgcaataca gcttgaagtc
     6361 atagctgccc accagcacaa aagtggggcg ggcgtctcgc ctgccaccag gggcaccctc
     6421 caccgcagcg gaggcggtcg gtgcggcagg cgagacgctc gccccactac cggcattcac
     6481 cttcacccaa ttcggagctc cgaggatctt gtctcccgtc tcgtatttcc aggccagctt
     6541 gccggtcccg gcctccagcg cgtaaagcca cccgtcgctc gaacccacaa acactttgcc
     6601 ctccaacacg aggggcgaag actcaactcc gcccccggtc ttgaaggccc agaccttctt
     6661 gccatcggcc agcgccagcg catagacgtt gccgtcgtcc gacccgatga agacacggtc
     6721 ctggacaatg gcagcggagg atttgactgg cccggcggtc ttgaaagtcc aaagcaggtc
     6781 cagcttttcc ggcaggctgc ccccggcgac acccgccagc gccggcccgc cccggaacat
     6841 cggccaatcg tcgcccggcg ccgcagcgct cgcgcaccag gacaccagca gccaggacca
     6901 aagaacgata tgccgcgcta agttcataaa tgccaggatg taagcccgaa aagtatcctc
     6961 caggccaata gccttcgcaa accaaaacaa ccaggccccg gctccgctgg ctttggtgag
     7021 gctttggggt agccttgggg tagccttggg gtagccttgg tgtagccttg gggtggcctt
     7081 ggtgtagcct tggggtggcc ttgaggtggc tttggggtgc ctatcagttg gctatcaaca
     7141 ggccttgggg tggccttggg gtggctttgg gtggctttgg gtggctttgc gcaaccctct
     7201 ttctgatttc agctttcagc tttcagtatt tcagcttttg cccaattgtg gctttggcga
     7261 gcttttccgg cccacaaaag ttcaaggttc gaagttcgag gttcaaggtt cgaggttcgg
     7321 gtcccatccg accaaaccga ccatcctccc cccgtgtttg agttggattg cgaactccac
     7381 gctgctgcgc ctggcgaagt tgttggcggt aaagtcaatc caccgcaggg ctttcttttc
     7441 ggaatgcgga aggaaaagcc agtgggtcag gtaggcgacc ttccggctcc gggccagccg
     7501 ctgcacaacg ggcgcatcct ttgccatgag gggccgcaat tcccgggagc tggtccgggc
     7561 aatggatgcg gaaaagtggt tcgggctcat gcctgaggcc gtgggagcgg ccaaagcggc
     7621 ccgggagagc ggggcggcgg ggaagatcgt ggcgctgccg aaggtgcccg gccagagggc
     7681 tagaggctcc ggggcggttg accggggaaa tacggtgtgg tcgggcacgc ggacagccgc
     7741 cagtgaagga gacgagcgaa tttcactcta aaagcaactg gact
//
LOCUS       DATCGS010000112         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_505918, whole genome shotgun sequence.
ACCESSION   DATCGS010000112 DATCGS010000000
VERSION     DATCGS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505918"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VIL39_02215"
     CDS             <1..715
                     /locus_tag="VIL39_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HEY5480271.1"
                     /translation="PLAVTLSLGERTEAELETWRNAGADRYLLRFETSDPKLFELIHP
                     SRDQRAGGRIPLLRHLKQLGYEVGSGIMVGLPGQTYDSVARDIDLFRELDLDMIGIGP
                     FIPHPQTPLGNGELKPVAAAGCQAPNTELMVYKAVALTRLVRPDANIPSTTALATINK
                     RNGRELGLQRGANVLMPNLTPVKYRKLYEIYPAKACIEETGSACNGCLRSRINVIGRQ
                     IARGPGGRKRRAPEVEVVC"
     gene            999..1262
                     /locus_tag="VIL39_02220"
     CDS             999..1262
                     /locus_tag="VIL39_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY5480272.1"
                     /translation="MSPEPLNSIMICMGSSCFSRGNNRNIEVIQEHLSTQPLPAGVEL
                     TGHLCEGHCKAGPNVTINGKMYHEVDPIVIIGLLNHYSQQREG"
     gene            1264..>2891
                     /locus_tag="VIL39_02225"
     CDS             1264..>2891
                     /locus_tag="VIL39_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480273.1"
                     /translation="MPVNFLNPIYTEKRECQDCYKCVRHCPVKAIKVESGYASVVPEL
                     CILCGHCVEVCPNAAKRVRDDLPQARQLLALKPRVVVSLAPSYVSEFPELAPGQLIRA
                     FKRLGFFGVSETALGAQQVSAQAAAALAEGGSRVLYSSACPTVVSYLQKHRPADSQFL
                     TGLLSPLLTHCKMLRQSFGPDIGIVFIGPCIAKKLEAAQHPTLLDVALTFDDLRRWLA
                     QEKLAPETLTPEAGDTFIPENSAEGAWYPVDGGMIAGMKSICAVNDCSFMAFSGINAI
                     QKAIEGLGTQPADGSLFLELLACEGGCVNGPRAAKRHGTAAKRRRVLRPIQAPVPVFP
                     RQSSLDIQSVFAVAPQPLLKFSDAQIREALRGVGKLTRDDELDCGGCGYESCREFAGA
                     LIQQKAERAMCVTYMRKLALKKANALIQKMPSAVVIINDAMRIIEFNTAFATMFACNV
                     APVGSPPAPGSLEGVMLSEVMPFANLFHAVLKSGEDILDRDLRYQKTILHAGIFTIEK
                     HCVVGGILQDITEPAVRKAEVIRKAQEVIQRSLQT"
BASE COUNT          555 a          951 c          885 g          500 t
ORIGIN      
        1 cccgctcgcc gtcactctga gcctgggcga acggactgaa gccgaactgg agacgtggcg
       61 caacgccggg gcggatcgct acctcctgcg ctttgaaact tcggacccaa agctgttcga
      121 gctgattcac ccgagccgtg accagcgcgc cggcggccgc attcccctgc tccgccacct
      181 caagcaactc ggttacgagg tgggcagcgg gataatggtc ggcttgccgg gccagacgta
      241 tgatagcgtc gcccgtgaca ttgatctgtt ccgcgaactg gacctggaca tgattggcat
      301 tgggccgttc attccgcatc cccagacccc gctgggcaac ggcgaactca agcccgtcgc
      361 cgctgccggc tgccaggcgc ccaataccga attgatggtc tataaggcgg tggcgctcac
      421 gcggctggtg cgtccggacg ccaacatccc cagcaccacg gccctggcca ccatcaataa
      481 gcgcaacgga cgggaactcg gcctgcaacg cggcgccaat gtgctcatgc cgaatctgac
      541 accggtaaaa taccggaagc tttacgagat ctatccggcc aaagcctgca tcgaggaaac
      601 cggcagcgcc tgcaatggct gcctgcgttc tcggattaac gtgattggcc gacagattgc
      661 ccgcggtccg ggcggccgga agcggcgcgc accggaggtg gaggtcgtat gttaggccgc
      721 agttccaatt tagcccaggg cgcgcgtcga aacccgaagg ccgaggcccg aaggccgaaa
      781 gaaggccgaa atccgaaatc cgagaaacgg cggtgccctc atcgcgcatg ccggccactc
      841 gaaggccatc ctcaccaggc ggtgctgggg aatgcaacgg actgcggctg gttgcggtat
      901 ggcgacttcg gtcttcgggt ttcggccttc tttcggcctt cgggtctcgg ccttcgggtt
      961 tggtctcaca agggccgtaa gtaagcaaaa tcaccgttat gtcacctgaa cctctaaatt
     1021 ccatcatgat ctgcatgggc agctcgtgct tttcgcgcgg gaacaaccgc aacatcgaag
     1081 tgattcagga gcacctcagc acgcagccgc tccccgctgg ggtcgagttg accggccacc
     1141 tgtgcgaagg ccactgcaag gcgggcccga acgtcaccat caacggcaag atgtatcacg
     1201 aggtggaccc catcgtgatc atcggcctgc tcaaccatta cagccagcag cgcgagggat
     1261 gaaatgcctg tgaacttcct gaatcccatc tacaccgaaa agcgcgaatg ccaggattgc
     1321 tacaagtgcg ttcgccactg cccagtcaag gccatcaagg tcgagagcgg ttacgcctcg
     1381 gtggtgccgg agctgtgcat cttgtgcgga cactgtgtgg aggtctgccc gaatgcggcc
     1441 aagcgggtgc gggatgatct gccccaggcg cggcaactcc tcgccctcaa gccgcgcgtg
     1501 gtggtatcgc tggctccctc gtatgtcagc gagtttcctg aactagcgcc gggccagctc
     1561 atccgcgcct tcaaacgcct cggcttcttc ggcgtgtccg agaccgccct gggcgcgcaa
     1621 caggtttccg cgcaagcggc ggcagccctg gcagagggcg ggagtcgcgt gctctactcc
     1681 tcggcctgcc ccacggtggt cagctacctg cagaaacatc ggcccgctga ctcgcaattc
     1741 ctcactggct tgctttcgcc gctgctgacc cactgcaaga tgctgcgcca gagcttcggg
     1801 ccggacatcg gcatcgtctt catcggaccg tgcatcgcca agaagctcga agccgctcaa
     1861 catccgacgt tgctcgatgt cgcgctcacc ttcgacgatt tgcgccggtg gctggcgcag
     1921 gagaagctcg cgccggaaac actgacgccc gaggccggag acacgttcat tccagagaac
     1981 tccgcggaag gcgcctggta cccggtggac ggtggcatga tcgcgggaat gaaatccatc
     2041 tgtgcggtga atgattgttc gtttatggcc ttctccggca tcaacgccat ccagaaagcc
     2101 atcgaaggcc taggcaccca gccggccgac ggcagcctgt tcctggagtt gctggcgtgc
     2161 gagggcggct gcgtgaacgg cccccgggcc gccaagcgcc acggcaccgc ggcgaagcgc
     2221 cgccgggtgc tccggcccat acaagcccct gtgcccgtgt tcccacggca gtccagcctg
     2281 gacatccaat ccgtgttcgc cgtggcgccc cagccgctgc tcaagttctc cgacgcacaa
     2341 atccgggaag ccctgcgggg cgtcgggaag ctcacgcggg atgacgagct ggactgcggc
     2401 ggctgcggtt acgaatcgtg ccgcgagttc gccggggcgc tgatccagca gaaggcggaa
     2461 cgcgccatgt gcgtcaccta catgcgcaag ctcgcgctca agaaggccaa cgcgctgatc
     2521 cagaagatgc cctcggccgt cgtcatcatc aacgacgcga tgcgcattat cgaattcaac
     2581 accgccttcg ccaccatgtt cgcctgcaac gtggcgccgg tgggctcgcc gcctgccccc
     2641 gggtcgctgg aaggcgtgat gctcagcgag gtcatgccgt tcgccaacct gttccacgcc
     2701 gtcctcaaga gcggcgagga catcctggac cgcgacctcc gctaccagaa aaccattctg
     2761 cacgcgggga ttttcaccat cgagaaacac tgcgtggtgg gcggcatcct gcaggacatc
     2821 acggaaccgg cggtccgcaa ggcggaagtg atccgcaaag cacaggaggt catccaacgc
     2881 agcctccaga c
//
LOCUS       DATCGS010000113         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_506033, whole genome shotgun sequence.
ACCESSION   DATCGS010000113 DATCGS010000000
VERSION     DATCGS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506033"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..142
                     /locus_tag="VIL39_02230"
     CDS             5..142
                     /locus_tag="VIL39_02230"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480274.1"
                     /translation="MACEDVAALAPLVLRHRLIRTFQADAEGKTTDEIIGQLLQDVAR
                     E"
     gene            185..1102
                     /locus_tag="VIL39_02235"
     CDS             185..1102
                     /locus_tag="VIL39_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5480275.1"
                     /translation="MPTATQTLSERFDPKMLAALEGLDFKARYVMEGFLSGLHDSPFH
                     GFSVEFSDYRNYQPGDDLRHLDWRLFARSDRLCIKRYMQETNARFYVVCDTSASMRYR
                     GGAAWGSKLECAKVLAAALTWFLLKQNDAAGMVTLSGESEVPEFIRPSQRPNQFGLML
                     RQLESLNASGGACLSRLIQHTVRLVHRRSVILFFSDLLEPSEEVARGFKRLRFHGHEV
                     IIFQVLDRDETEFPFTESRLFEDMETGARRMVTPAAARESYLARFNAFMQVHRELFRS
                     LEMTHCVVRTDENPWRALALFLAERKRLK"
     gene            1113..3152
                     /locus_tag="VIL39_02240"
     CDS             1113..3152
                     /locus_tag="VIL39_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY5480276.1"
                     /translation="MPFSFLNPWFWLGALAVAAPIWLHLRRKRETNLVEFSALQFLDD
                     QPEPRRSPLRLRDLLLFALRVLALLLLVSAFAWPYLRGANTAPVKESRVYILDNTLSH
                     QANNGFARDRDRVLSEVSKGPSDIQMAVIELTSAPRVVVSFGDSREAAKQKLKELEPS
                     YQRGSYLPAFRQANSLLGNSLGEEKRIVLLGDNQENQWTENVNSPPFLRNVQIDLPKI
                     TALAQPNLSLSEPRVQRIFLGDKSLVNFTVKLSHKGEAKTADIVLRANEQVIFNRAVE
                     LEQQPDTILLQAQWEADPAAWLRGEVTVDGTPDALAGDNRVFFSLAPVVEGKVALLAQ
                     SQYLRLALSPEVMRGQWATRVLDPTKLAAELAANEDADVLVVESNYLQSTDARKLLWR
                     YVTNGRGVVLLVNRVTPSISGCLRELGFEAESTVRAENGKAERFQFVYSNHPIFHPFL
                     SPDYGSLMDVKVSKYVQLKGSQAMPLVFSEKGAGLFFQGTKFPGKLFVAAFGLDREHT
                     SWPIDQTFVPFLDLTLQTARAEDPTPTTFEPGEISKIQLPVGSVAKEAVLRDERQVLS
                     RAPVEQGRTQVRMPNKPGVYTLTYDDGAQVEKMFSVNPSPKESQLAYVEAPETLKAWR
                     VTLPPGAGKAATAAARGKVSLSGILQQHWWWWMVWGGLAALMLEMALAEIGRERV"
BASE COUNT          583 a          862 c         1127 g          588 t
ORIGIN      
        1 taacgtggcc tgcgaggatg tcgcggcact ggcgccgctg gtgctgcggc atcggttgat
       61 tcgcacgttc caggcggatg cagagggcaa gacgacggat gagatcatcg ggcaactatt
      121 gcaggatgtg gcgagggaat agggagttaa atgagttaca tgggttacat tagttaaatg
      181 ggttatgccc acggcgacgc aaacattgtc tgagcggttc gatccgaaga tgctggcggc
      241 gctggagggg ctggatttca aggcgcgcta tgtgatggag gggttcctga gcgggttgca
      301 cgacagcccg tttcacgggt tcagcgtcga gttcagcgat taccggaatt accagccggg
      361 agatgatttg cgtcacttgg actggcggct gttcgcgcgg agcgaccggc tctgcatcaa
      421 gcgctacatg caggagacca atgcgcggtt ctatgtcgtc tgcgacacga gcgcctcgat
      481 gaggtatcgg ggcggggcgg cttggggctc gaagctcgaa tgcgccaagg ttctcgcggc
      541 agcgctgacg tggttcctcc tcaagcagaa cgatgcggcg ggcatggtga cgctgagcgg
      601 cgagagtgaa gtgccggagt ttatccgccc gtcgcaacgg cccaaccagt ttgggctgat
      661 gctgcgccag ctcgagtcgc tgaacgcgag cggcggggct tgcctgtcgc gcctgatcca
      721 acacaccgtc cgcctggtgc atcgccgcag cgtaatcctg ttcttctccg atttgctgga
      781 gccctcggaa gaggtggcgc ggggattcaa gcgactgcgg ttccacggcc atgaggtgat
      841 catcttccag gtgctggacc gcgatgagac ggagttccca ttcacggagt ccaggctgtt
      901 cgaggatatg gagacgggcg cgcggcgcat ggtgacgccg gcggcggcgc gggagagcta
      961 cctggcgagg ttcaatgcgt ttatgcaagt ccaccgggag ttgttccgca gcctggagat
     1021 gacgcattgc gtggtgcgca cggacgagaa tccgtggcgg gcgctggcct tgttcctggc
     1081 cgagaggaag cgcttgaagt agtcaagtcg gcatgccctt ttccttcctc aacccatggt
     1141 tctggctggg cgcgctggcg gtggcggcgc cgatctggct gcatttgcgg cggaagcggg
     1201 agaccaacct ggtcgagttt tccgccttgc agttcctgga cgaccagccc gagccgcgcc
     1261 gcagcccgtt gcgcctgcgg gacctgctgc tgttcgcgct gcgggtgctg gcgctgctgt
     1321 tgctggtgtc ggcgtttgcc tggccttatc tgcgcggagc caacacggcg ccggtcaagg
     1381 aaagccgggt ctatattctc gacaacaccc tgagccacca ggctaataac ggctttgcgc
     1441 gcgaccgcga ccgggttctg agcgaggtta gcaaggggcc aagcgacatc cagatggcag
     1501 tgatcgagct gacctcggca ccgcgcgtgg tggtctcgtt cggagacagc cgcgaggcgg
     1561 cgaagcagaa gctgaaggag ttggagccgt cctaccagcg tggctcctac ctgccggcgt
     1621 tccgtcaggc caactccctg ctgggcaatt cgctgggcga ggagaagcgc atcgtcctgc
     1681 tgggcgacaa ccaggagaat cagtggacgg agaacgtgaa cagcccgccg tttttacgca
     1741 acgtgcagat tgacctgccg aagatcaccg cgctggcgca accgaacctt tcgctgtccg
     1801 agccacgcgt gcagcggata ttcctgggcg acaaatcgct ggtgaacttc acggtcaagc
     1861 tcagccacaa gggcgaagcg aagacggcgg acattgtgtt gcgcgccaac gagcaagtca
     1921 ttttcaaccg ggcagtggag ctggagcaac agccggacac cattctgctg caagcgcaat
     1981 gggaagccga cccggccgcc tggctgcgcg gcgaagtgac cgtggacggg acgccggacg
     2041 cgctggcggg ggacaaccgg gtgttctttt cgctcgcgcc ggtggtcgag ggcaaggtgg
     2101 cgctcttggc gcaatcgcag tatctgcggc tggcgctgtc gccggaggtt atgcgcggcc
     2161 aatgggcgac ccgggtgctg gacccgacca agctggcggc ggagctggcc gcgaacgagg
     2221 atgccgatgt gctggttgtg gagagcaact acctgcaatc aaccgacgcg cgcaagctgc
     2281 tctggcgtta tgtgacgaat gggcgcggcg tggtgttgct ggtgaaccgg gtgacgccca
     2341 gcattagcgg ctgcctgcgg gagctgggct tcgaggctga aagcaccgtc agggcggaaa
     2401 acggcaaggc ggagagattt cagttcgtgt attcgaacca tccgatcttc catccgttcc
     2461 tgtcgccgga ttacggcagc ctgatggatg tcaaggtttc caagtatgtg cagctcaaag
     2521 ggagccaggc catgccgctg gtcttctccg agaagggcgc ggggctgttc ttccagggaa
     2581 caaagtttcc ggggaagcta ttcgtggccg cgttcgggct ggaccgggag cacacctcgt
     2641 ggccgattga ccaaaccttt gtgccgtttc tcgatctgac gctgcaaacg gcccgggcag
     2701 aggacccgac gccgacgacg tttgagccgg gagagatcag caagatccaa ctgccggtgg
     2761 gctcggttgc caaggaagcc gtgttgcggg atgagcggca ggtgctgagc cgcgcgccgg
     2821 tagagcaggg ccggacacag gtgcgaatgc cgaataagcc gggggtctat acgctgacgt
     2881 acgacgacgg cgcgcaggtg gagaagatgt tcagcgtgaa tccctcgccg aaggaatccc
     2941 aattggcgta tgtcgaagcg cccgagaccc tcaaggcgtg gcgggtgact ctgccgcccg
     3001 gagccgggaa agccgcgacg gcggcagcgc gtgggaaagt gagtctgagt ggcatcttgc
     3061 aacagcattg gtggtggtgg atggtgtggg gaggtttagc ggcgctgatg ttggagatgg
     3121 cgctggccga gataggacgg gagcgtgtat gagtaaccga
//
LOCUS       DATCGS010000114         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_159666, whole genome shotgun sequence.
ACCESSION   DATCGS010000114 DATCGS010000000
VERSION     DATCGS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159666"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2551)
                     /locus_tag="VIL39_02245"
     CDS             complement(<1..2551)
                     /locus_tag="VIL39_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY5480277.1"
                     /translation="MTFGAVGFAKGEAPAAVPDAKTLDRAFGNEDAAAFREPPRVFRP
                     ETWFHFIGGNVATQGITADLEAIAGAGIEGVQLFHGQFGGPWPGVEPQIKCLSESWDG
                     AVRHTAEECRRLGLRFTMQNCPGWAMAGGPWITPDKAMRHLVWSRTDVTGGGSGTVSL
                     PQPQPSQEEWRDYREVAVVAFPTPEGDTGKALAPVSVRSNREDLPWEKCLGKEKDGKI
                     VLEPGKEPVWVEATFEEAVTLRTVEFPSVQSFNHNWCYVPGVTITVQAVLPAGMREVA
                     RHEMPESNWQDNKPITLACSEVPAKTYRITIDNQHSMTFSYLQLFTGARKNNWEAEAA
                     GCLRGLDRAPHPQQAKTAWLDPARIVDLADKMDARGTLRWKAPPGRWTVLRWGHVNTG
                     RRNGPAPPEATGWECDKLSPAGAEAHFAGYIGRLAAKNGPVGGGLLQGMVLDSWECET
                     QTWTPGMDQQFARLRGYPLRPWLPALAGYVVGDPETTTRFLRDWRATVNALLVENFFG
                     RMAALGHQNGLAISFETASGDVFPGDILEYYKYADVPMCEFWQPRSEAFVGSFEFKPV
                     KPCVSAARMYGKPRVAAEAFTSFNLTWNEHPGMLKHIADMHLAEGVTHLVFHTYTHNP
                     RTDFLPPGTAFGSGIGTPFLRGQTWWAHLPEFTAYLARCGYLLERGQPVSDVLWYLGD
                     EQDHKPRQDAPFPPGYRYDYCNPDVLLHRLSVRDGMIVTPEGLRYRALWLRDCPRLLP
                     ETLERMLSLVKQGAVLVGERPRGLATLSGGDRAEKRFHNAVVALWGAEGRPSAAATTN
                     GVRHLGKGTVICGVPLGDALAKLGIEPDVQGEGVVWTHRQADGADWYFVAAP"
BASE COUNT          509 a         1018 c          819 g          472 t
ORIGIN      
        1 cgggagcagc cacaaagtac cagtcggccc cgtctgcctg ccggtgcgtc cagaccaccc
       61 cctcgccctg gacgtcaggc tcgatgccga gttttgccaa cgcgtccccc agaggcaccc
      121 cgcagatcac cgttcccttg cccagatgcc gcaccccgtt cgtcgtcgcg gctgcagacg
      181 gtcttccctc cgcaccccac agcgccacga ccgcgttgtg gaaccgtttc tccgcccggt
      241 cgccgccgct cagcgtcgcc aggccccggg gacgctcacc gaccagcacc gcgccctgtt
      301 tcacgagcga caacatgcgt tcgagcgtct ccggcaacag ccgcggacaa tcgcgcagcc
      361 acagcgcgcg atagcgcaag ccttccggcg tgacgatcat tccgtcgcgc accgacaacc
      421 ggtggagcag gacatccgga ttgcagtagt cgtagcgata gccgggcgga aacggcgcat
      481 cctgccgtgg cttatgatcc tgctcgtcgc ccagatacca gagcacgtcc gaaaccggct
      541 gcccacgctc cagcagatag ccgcatcggg ccagatacgc cgtaaactca ggcaggtgcg
      601 cccaccaggt ctgaccgcgc aggaagggcg tgccgatccc cgagccaaac gccgtgcccg
      661 ggggcaggaa gtccgtgcgc gggttgtggg tataagtatg gaacaccagg tgggtgacgc
      721 cttcggcgag gtgcatgtcc gcaatgtgct taagcatgcc gggatgctcg ttccaagtga
      781 ggttaaacga cgtgaaggct tcggccgcca cgcgcggctt gccgtacatc cgcgcagccg
      841 acacgcacgg cttgaccggc ttgaactcga aactgcccac aaaggcctcg ctccggggct
      901 gccagaattc gcacatcggc acatccgcgt atttgtagta ctccagaatg tcgccgggaa
      961 acacgtcgcc tgaggccgtc tcgaacgaga tggctaatcc gttctggtgc cccagcgccg
     1021 ccatgcgacc gaagaagttc tcgaccagca aagcgttcac tgtcgcccgc cagtcgcgca
     1081 gaaagcgcgt tgtggtttcg gggtcaccaa ccacataacc cgcgagcgcc ggaagccagg
     1141 gccgcagcgg atagccgcgc aaccgcgcga actgctggtc catgcccggc gtccacgtct
     1201 gcgtctcgca ctcccaactg tcgagcacca tgccttgcaa gagcccaccg ccgaccgggc
     1261 cgttcttggc cgcgagccgc ccgatgtatc cggcaaagtg agcctcggcc cctgccggtg
     1321 agagtttatc gcactcccac ccggtcgcct cgggcggggc cggcccgttg cgtctgccgg
     1381 tgttcacatg cccccagcgc agcacggtcc aacggcctgg cggcgccttc cagcgaaggg
     1441 tcccccgggc atccatcttg tccgccaaat caacgatccg cgcgggatcg agccacgccg
     1501 tcttcgcctg ttgcggatgc ggcgcccgat ccaaaccgcg caaacaaccc gccgcctccg
     1561 cctcccaatt gttcttccgc gcacccgtga agagttggag gtaggagaaa gtcatgctgt
     1621 gctggttgtc tatggtgatc cggtaggtct tggcggggac ttcggaacac gccagcgtaa
     1681 tcggcttgtt gtcctgccaa ttgctctccg gcatctcgtg ccgcgccacc tcccgcatcc
     1741 cggccggcaa gaccgcctgc accgtgatcg tcacgccggg aacgtagcac caattgtgat
     1801 tgaagctctg aaccgagggg aactccaccg tgcgtagtgt aaccgcttcc tcgaaggtcg
     1861 cctccaccca aaccggctcc ttcccgggct ccagcacgat cttcccgtcc ttttcctttc
     1921 ccaggcactt ttcccagggc aggtcttccc ggttgctcct gaccgacact ggcgccagtg
     1981 ccttgcccgt atctccttcc ggcgtcggga acgccaccac cgcgacctcg cggtaatcgc
     2041 gccactcctc ctggctcggt tgcggctgtg gcagggagac ggtcccgcta ccgccgcccg
     2101 tcacgtcggt gcggctccag accaagtgcc gcatcgcctt atccggcgtg atccacggcc
     2161 cgccggccat cgcccagccc ggacaattct gcatggtgaa gcgcagcccc agccgccggc
     2221 actcctcggc cgtgtggcgc acggctccgt cccacgactc gctgaggcac ttgatctgcg
     2281 gctccacgcc cggccacggt ccgccgaact gcccgtgaaa gagctgaacg ccctcaatcc
     2341 ccgcgccggc gattgcctcc agatccgccg tgattccttg cgtcgccacg tttccaccaa
     2401 tgaagtggaa ccaggtctcg ggacggaaaa ccctgggcgg ttcacgaaaa gccgcggcat
     2461 cttcattgcc aaacgcccga tccagggttt tggcgtcagg gacggcggct ggcgcctccc
     2521 ccttggcgaa gccaaccgcc ccaaaagtca tccacagaac gctaaaacag cacgcccatc
     2581 taagtgtcat aataaagcaa gggagcggcg gcggcatcag aaagccttca aacatcgagc
     2641 ttcagaccgg cacgcttcac cgcaccacac ccgcgagagc gtaattgcca ccacggccat
     2701 tacaagatgc gagttgccct gttgcccgcc gcccaaggcc aattcatgaa ggggcgggga
     2761 cggcctagtc cccggttgtt ggcggggcga agattggtca accggggacg aggccgtc
//
LOCUS       DATCGS010000115         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_654513, whole genome shotgun sequence.
ACCESSION   DATCGS010000115 DATCGS010000000
VERSION     DATCGS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654513"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VIL39_02250"
     CDS             <1..305
                     /locus_tag="VIL39_02250"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5480278.1"
                     /translation="IDTTPPTVQINSPANGARLPAIGNVTIGVSAHDNIGVVQVSIYA
                     DGVQVYTGTSAPYSGHWNLNKAAPGSSHTISATAWDAAGNHASTSITVYINPPVRW"
     gene            complement(606..1763)
                     /locus_tag="VIL39_02255"
     CDS             complement(606..1763)
                     /locus_tag="VIL39_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5480279.1"
                     /translation="MMKRLSGLIFVIATGYTGICATRSLLPGNADRGKDLFRTLNCGV
                     CHSIGGEGGKSAPDLGRGVERGFSPYVLAGLLWNHAPVMWAAMERQGVAKPELSEQQA
                     ADLFVYFFAARYFEQLGDAKRGERVFLAKRCVECHGIGSALREGIQPVSAWKSLADPI
                     ALAQQMWNHSWEMKPVLDRMKIPYPRFSAQELTDLLVYLRSTQGPGHAGDFSPGSVES
                     GEELFVVKGCAGCHKGALTLEARPTRYSLTDFAAAMWNHPLRALPSPAPLSYEEMRRL
                     VGYLVSTQFFEERGDLEQGKKVFARKRCGACHDDPSSGAPGRSAMAGRMTSFEMVAAL
                     WKHGPAMLNRMRLKKIPWPRFSGSEMADLTAYLHGFNLKQRRPLIDPARHP"
BASE COUNT          405 a          665 c          581 g          373 t
ORIGIN      
        1 taatcgacac cactccgccc actgtgcaaa tcaattcgcc cgccaacggc gcgcgtcttc
       61 ccgccatcgg caatgtcacc atcggcgttt cggcgcacga caacataggc gtggtgcagg
      121 tctctattta cgccgatggc gtgcaggtct ataccggcac ctccgcgccg tattcgggcc
      181 actggaacct taacaaggcg gccccaggca gctcccacac tatcagtgcc accgcgtggg
      241 acgccgccgg caaccacgcc agtacgtcca tcacagtgta tattaatccg ccagttcgtt
      301 ggtagcagtc gtgagcgctg ggagccccga tttcatgggc tttcagcaat tccgatgtcc
      361 agctacttgt ggccccatta ataagtaggt gggaaaagcc tctccgtccc aattgccggc
      421 gcgggcggcg ggatcggggg tggattggtc gtttcagttg tctcgggcaa gtaccggacc
      481 cgtggcctcg tcgtgctcgg ccccgatcag gggagcgagt cattggacga cacgccgatc
      541 gcggctggtt gcccgctctg cagcgggacc tccttggagc catcaaccgg aacagcgact
      601 gcggattacg gatgccgggc agggtctatc agtggccgcc gttgtttcaa attgaaaccg
      661 tgcaggtatg ccgtaaggtc cgccatctcc gagccgctga accgaggcca gggaatcttt
      721 ttcagtcgca tgcggttcaa catcgctggc ccgtgcttcc agagagcggc caccatctcg
      781 aaggatgtca ttctcccagc catagccgac cggcccggcg caccgctgga cgggtcgtca
      841 tgacaggcgc cgcagcgctt cctcgcaaaa actttcttgc cttgctccag gtcgccccgc
      901 tcctcaaaga actgcgtcga gacgaggtag cccaccagtc ggcgcatctc ctcatagcta
      961 agcggcgccg gactaggaag tgcgcggagc gggtggttcc acatggccgc ggcaaagtca
     1021 gtcaggctgt atcgggtggg gcgggcttcc agggtcagcg ctcccttgtg gcaaccggcg
     1081 cagcccttca ccacgaatag ctcctcaccg gactccaccg aacctggcga gaagtcgccg
     1141 gcgtggccgg gcccttgcgt gctccgcaga tacaccagca gatcggtcag ctcctgtgcg
     1201 gagaagcgcg gataaggaat cttcatccgg tcgagcaccg gtttcatctc ccaggagtga
     1261 ttccacatct gctgcgcgag cgcaataggg tccgcaagcg acttccaagc cgatactggc
     1321 tggatgcctt cgcgaagcgc agacccgata ccgtggcact ccacgcaccg cttcgcaaga
     1381 aaaacccgct cgccgcgctt ggcgtcgcca agctgctcga aataacgggc agcaaagaaa
     1441 tagacgaaca agtccgccgc ctgctgctca ctcagctcag gcttagcaac tccttgtctt
     1501 tccattgcgg cccacataac cggagcatgg ttccacagca ggcccgccag gacgtagggg
     1561 ctgaagcccc gctccacgcc ccgccccaag tccggcgcgc tctttccgcc ctccccgccg
     1621 atgctgtggc atacgccgca gttcagcgtg cggaataaat ccttgccacg gtccgcatta
     1681 ccaggcagca ggctcctcgt cgcacaaatc ccggtataac cggtggcgat tacgaagatc
     1741 aaacctgaaa gtcgcttcat cattcgaggc ggccaacaat ggggaagcct aaacggaaac
     1801 ggcgacccac agagcaatgc ctgccgggtc gccgtcgttc gcctcaacgc gcaactacgt
     1861 ttgggactgg gccgactcag cgcatcgcca gaacgatctc cgtggcagcg gcggtcttct
     1921 tcaactcgcg ttccacgcga gacatcggaa agtcgtggct tgtcgacgtg gtctgaatcc
     1981 gggacaagaa gtactgatcc ccgtaacgac ggaagaccag ctta
//
LOCUS       DATCGS010000116         3926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_110344, whole genome shotgun sequence.
ACCESSION   DATCGS010000116 DATCGS010000000
VERSION     DATCGS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110344"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIL39_02260"
     CDS             <1..284
                     /locus_tag="VIL39_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480280.1"
                     /translation="DYSVTTSVVYSQTNAVSSMVNNGDGTFTLNFVGTPQAKYYVVTS
                     PDPWALMSGWTVLPNSTNTAPSPSGIWSVTATNNASQRFYRSAAVHPAP"
     gene            426..1607
                     /locus_tag="VIL39_02265"
     CDS             426..1607
                     /locus_tag="VIL39_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480281.1"
                     /translation="MKAAIQGSWFRRGDRAYTLVELLCSIGIITLLAAMLLPALRKGY
                     GKAQRIYCVNNLRQTGLAFHSFAHAHQDRFPMQVSTNDGGSLEFLHAANTMTGEFYFS
                     YRHFPPLSNDLGTPKILDCPTDTRRPATNFAQLQNENLSYFAAGSPEFGSPNSVLAGN
                     RNVSPVYGSIATVGTYRRLRWTEELHRFKGNVLFSDGHVDQLNDVFSVTNRGVAAAPT
                     SLHLPTVKPDAAASGGSGSGGSGYDYAGGSGAAAPGVPGVPPMFTNRLGSNQPVITWT
                     VNGPSLKGRFLSAPGGNSAAAGGVSPAPDSRPSPPLARAASANPRVAAPDVDPWSLKG
                     VYQQVINKGHRMLRRAALGVYDIPWYLLLLLIVALLELRRRLRARQRNIVSRPFGGGG
                     S"
     gene            1648..2487
                     /locus_tag="VIL39_02270"
     CDS             1648..2487
                     /locus_tag="VIL39_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480282.1"
                     /translation="MLTTTGRPFAAMLGGMALACLLPAAACGASSQVAARLLPNSMLS
                     LIWTGQSGRYYRIQFKTNLADPVWRDVPGEIEGCDYGNSRILLTTNQPQRFFRIVDIG
                     DTSSFVPAYPDPSGNWVMFHRFTMELPATYTNDIFVTASGFALQRLSPVPIWEVFDGP
                     ATDNAALDQRIVYADLMADSRVVWANPGRTVPPFFSPLYYDNVLLTQLRPPFSDIGSL
                     LAEQHLSLIGYFAFVDTFLVGVTNKTDDAFIIAQRLQGDPRLNYIEVDGYGEPRDGSC
                     IAP"
     gene            complement(2647..>3926)
                     /locus_tag="VIL39_02275"
     CDS             complement(2647..>3926)
                     /locus_tag="VIL39_02275"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEY5480283.1"
                     /translation="LQGSPKSKVQSPKSKVQSPKSKARSPKASDVQGSRFEVQGSKFP
                     SSLLPPPSSPSLVVTPRPRYIPDYATPRFRLTPRHFAYVKIAEGCNHPCSFCIIPRIR
                     GSHRSRPQGDLVAEARALIADGVKELNLISQDSTYYGMDLRPNRTGAISAPAIFNAAV
                     KSLPAQAATICTLLRELNALPGDFWIRLLYTHPAHWTDELIQTIADCPKVARYVDIPL
                     QHIHQNMLERMRRETSSQYIMDLIRRIRAGIPGIALRTAFIVGFPGETEAHFAALLDF
                     IREAKFERLGVFTYSQEEDTRAGKMAGQVTDRLKQKRRGLAMATQHEVAVQVAESFVG
                     RTIKVLVEQAASLVELRRARVGSWEHGLIRGQDRHAAALKGRFLVARGEADAPDIDGR
                     VYVRGQLPLSEFAQVKVIGHTDYDLIAESNALR"
BASE COUNT          713 a         1183 c         1247 g          783 t
ORIGIN      
        1 tcgactactc agtgacgact tctgtggttt acagccagac caacgcggtg tcctcgatgg
       61 taaacaacgg ggacggcacc ttcaccttga acttcgtggg cacgccgcag gcaaagtatt
      121 acgtggtgac aagccccgac ccgtgggctc tcatgtccgg ctggacggtc ttgccaaaca
      181 gcacgaacac ggcgccgtct ccgagcggca tctggtcggt cacggcgacc aacaatgcct
      241 cgcagaggtt ctatcgctcg gcggcggtgc atccggcgcc ataggctcgc gagcacgggc
      301 tgctcggaaa gctgcctgcg gtccaaaccg ccgtccaacg gcatatcgga gcctgggcgg
      361 ctcagatgct attgggattg acggtttgaa cggaagctgc gtaacgtcgg gcaggccggg
      421 gtattatgaa ggcagctatt caaggttctt ggttcaggcg gggcgatcgg gcttacacgc
      481 tggtcgaact gctttgcagc attggtatta tcactctcct cgccgccatg ctcctgccgg
      541 cgttgcgcaa gggatacggc aaagcccagc gcatctactg cgtcaacaat ctccgccaga
      601 cgggcctggc gtttcatagc tttgctcacg cgcaccagga ccggttcccg atgcaggttt
      661 cgaccaatga cggcggctcg ctggagttcc tccatgccgc caacaccatg acgggcgagt
      721 tctatttctc gtatcggcat tttcccccgc tctcgaacga cctggggacg cccaagatcc
      781 tggactgccc tacggataca cgccgcccgg ccacaaattt cgcccagctc caaaacgaga
      841 acctgagcta cttcgccgcc ggcagcccgg agttcggcag tcccaactcg gtcctggcgg
      901 gcaaccgcaa tgtctcgccg gtctatggca gcatagccac cgtcggcacc tatcgccgct
      961 tgaggtggac ggaggagctg catcggttta agggcaacgt cctttttagc gatggccacg
     1021 tggaccagtt gaatgatgtg tttagcgtca cgaaccgcgg ggtggccgcc gcccccacga
     1081 gcctgcacct gcccacggta aagccagatg cggctgcgtc cggcgggagt gggagcggtg
     1141 gcagcggcta cgattatgct ggcggcagcg gggctgcggc gccgggggtt ccgggcgtcc
     1201 cgcccatgtt cacgaaccgg ctggggagca accagccagt gatcacctgg acggtgaacg
     1261 ggccatcgct caagggccgg tttttgagtg ctcccggggg caacagcgcg gctgccggcg
     1321 gtgtgtcgcc cgcgcccgat tcgagaccca gtccgccgtt ggccagagcc gccagcgcaa
     1381 acccgagagt ggcggccccc gacgttgacc cctggagcct gaaaggggtt tatcagcagg
     1441 tgatcaataa aggccatcgg atgctccgcc gcgcggcttt gggggtctat gatattccgt
     1501 ggtatctgct gctgctcctg atcgtcgcct tgttggagct ccgccgacgc ctgcgagccc
     1561 ggcaaaggaa catcgtgtcc cgcccttttg ggggcggggg ctcgtagggt ccgccaagga
     1621 agaatgagcg cgaccaaaga cgtatttatg ctgaccacga cagggcggcc atttgccgct
     1681 atgcttggag ggatggcgct ggcttgtctg ttgcctgccg ccgcttgcgg ggcaagttcc
     1741 caggtcgccg cccggctttt gcctaattcc atgctgtcgc tgatttggac tgggcagtcg
     1801 gggcgttatt accgcatcca gtttaaaacg aatcttgctg atccggtctg gcgggacgtc
     1861 ccgggtgaga tcgagggctg cgactacggg aacagccgaa ttctgctgac caccaaccaa
     1921 ccgcagcgct tctttcggat agtcgatatt ggggacacga gttccttcgt ccccgcttac
     1981 ccggatccga gcggcaactg ggtgatgttc caccggttta ccatggaact gccagcaacc
     2041 tacaccaacg acatctttgt aaccgccagc gggtttgccc ttcaaaggtt gtctcccgtt
     2101 ccgatttggg aggtgtttga cggccccgcg acggacaacg ccgctttgga ccagcgaatc
     2161 gtttacgctg atctaatggc ggacagccgg gtggtttggg ccaacccggg tcgcacagtg
     2221 ccgcctttct tttccccgtt gtattacgat aacgtgctgc tcacacagct tcgccctccc
     2281 ttttcggaca tcggcagttt gctggcggaa cagcatctga gtttaatcgg ttacttcgca
     2341 ttcgtcgaca cgtttctcgt cggcgtcacg aacaagacgg acgacgcgtt tatcatcgca
     2401 caacgcctgc agggagatcc gcgtctgaat tatattgaag ttgatggtta cggagagcca
     2461 agggacggat catgcatcgc accgtgaggg ggtctcgcct catgttcctc gctcctgcag
     2521 cctgaaggct gcgctcctat ccaggacttg ccgagcctga aacggcccct caccccgacc
     2581 ctctccccat cggatggggc gagggagaag tggtggacgc cgatggagta tgctgaatgt
     2641 cgcgagttac cggagcgcgt tggattcggc gatcaggtcg tagtcggtgt ggccgatgac
     2701 cttcacctgc gcgaattcgc tcaggggcag ttgaccgcgc acgtacaccc ggccgtcaat
     2761 gtccggggca tcggcttcgc cgcgggcgac caggaaccgg cccttgagcg cggcggcgtg
     2821 ccggtcctgc ccgcgaatca ggccgtgttc ccacgaaccg acgcgggcgc ggcgcaactc
     2881 caccaggctg gccgcctgct cgaccagcac cttgatggtg cgcccgacga atgattcagc
     2941 gacctgcact gcgacctcat gctgcgtcgc catggccaag cctcggcgtt tctgcttaag
     3001 ccggtccgta acctggcccg ccatcttgcc ggcacgcgtg tcttcctcct gcgaataggt
     3061 gaagactccc aggcgctcaa acttcgcctc ccgaatgaag tcgagcaacg cggcaaaatg
     3121 agcctccgtc tcgccaggga acccgacgat gaaggccgtg cgcagtgcga tgccgggaat
     3181 acccgcgcga atacggcgga tcaggtccat tatgtactgc gaagaagtct cgcgccgcat
     3241 ccgctcgagc atgttctggt ggatgtgctg caacgggata tccacgtagc gcgcgacctt
     3301 ggggcagtcg gcaatggtct gaatcaactc atccgtccag tgcgcgggat gggtgtagag
     3361 caggcgaatc cagaaatcgc ccggcaaggc attcagttcg cgcaacaggg tgcaaatcgt
     3421 ggcggcctgg gccgggagtg acttcaccgc cgcgttgaat atcgcgggcg cggagatggc
     3481 gccggtgcgg ttcgggcgca agtccatgcc gtaataagtc gagtcctgcg agatcaggtt
     3541 cagctccttc acgccgtcgg caatgagggc cctggcttcg gccacaaggt ccccttgcgg
     3601 acggctgcgg tgcgagcccc tgatgcgggg aatgatgcag aagctgcatg gatggttaca
     3661 tccttcggcg atcttgacgt aggcaaagtg gcgcggcgtc agccggaagc gcggcgtcgc
     3721 gtagtcgggg atgtagcggg gccgcggggt gacgaccagg gagggcgagg agggaggagg
     3781 gaggagggag gaggggaact tcgaaccttg aacttcgaac cttgaacctt gaacgtctga
     3841 agccttcggg cttcgtgcct tggactttgg actttggact ttggactttg gactttggac
     3901 tttggacttt ggactgcctt ggagca
//
LOCUS       DATCGS010000117         4839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_555644, whole genome shotgun sequence.
ACCESSION   DATCGS010000117 DATCGS010000000
VERSION     DATCGS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555644"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VIL39_02280"
     CDS             <1..200
                     /locus_tag="VIL39_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480284.1"
                     /translation="ALVDYQQTRLQLLLDIGALDTEAPKFWLKDNLAAYLPGKTPAPG
                     NPQPPDSGKQSVLPPDQYFSE"
     gene            207..2072
                     /locus_tag="VIL39_02285"
     CDS             207..2072
                     /locus_tag="VIL39_02285"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480285.1"
                     /translation="MNVQTLYQGLVRRLKERPRLAAGLGLAVLVLGVMVFARPRPQTQ
                     AVSCYEVKSGDMLVSVVEGGTLEAVSEVSIRSEVEGTARIIFIVPEGSNVKKDDLLVE
                     LDSSNSEDAVNLQQINVERAQFALVQAEQQLAIQKSVVDSEIQAATLKVEFSESDLEK
                     FTNGLALQTERNAQIAITNVLETLQIDRDRLNWSEKLYKQGFETKANLDKDRLTASQD
                     QLRLEQAEKALWMVETFDNPKTKRSLEATLQEAKENLDRVKLQDERKLAQFAADVDSQ
                     KKTLALSQEKLERDKKQLVATKIYAPQDGLVVYAGSGGGHWSNESMIEEGAVVRNRQE
                     IIKLPDVSAMKVQVKIHETYINQVALGQPAFVVLDSMPDQRFCGNVSKVAPLPDSQSR
                     WGNPDLKVYATEILVTDKLPDIKPGVSARAEVLITNLVNVVSVPIQAVTTRKGKQVVF
                     LASAPQEPVPVTVGQYNTKFIQICSGVRDGDLVMLAPPFDTKEKDLGGAIVAEGETLP
                     PGVTNQIARAKAPGKDKGAKDRSRPGSDNMRPILVVADTDQGDRPRLDNPRSPKSERP
                     GRTEFSGSSTNQADLVKRFDADGDGKLNAVERAEMERWLARRRGTNAPSNGPVLN"
     gene            2079..2786
                     /locus_tag="VIL39_02290"
     CDS             2079..2786
                     /locus_tag="VIL39_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5480286.1"
                     /translation="MQTTEPIIRLESLEKRYDLGGEAEVRALRGVNLVVNPGSYVAIM
                     GPSGSGKSTMLNLIGCLDRPTSGHYFLGGTDVSRMPDDELSEARGARIGFIFQSYNLI
                     AQLTVIENIQVPLLYQGKDLHAYRDHCVKLASMVGLAERLHHRPNQLSGGQQQRVAVA
                     RSLVNDPLLILADEPTGNLDSRTGQEVLELIDTLNAQGKTIVLVTHDERIATRAHRVL
                     HMRDGLIESEVVNRPDP"
     gene            2783..4075
                     /locus_tag="VIL39_02295"
     CDS             2783..4075
                     /locus_tag="VIL39_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480287.1"
                     /translation="MNPLSLQFITTVRLGVKSLMLHKLRSVLTMLGIIFGVCSVIAML
                     AIGEGASYEAQQAIKKLGSQNIIIRSLKPPEDAKMSGMGRGIALEYGLTYDDGSRIKS
                     TIPGVVRILPVRIIRENVRFARNSVPCQIIGTLPTYTEIVGLDIVRGRFLSETDQINH
                     DNVCVLTAGLAQRLFPYQDPLTESIKVNSFYYRVVGLVREKNMPEQRTQAGKMEGEPL
                     DNNVYITLSAARSRFGDVIVRRTAGSLEVEKVELHQITVQMQDVAAVEAADPQIKTLL
                     GRFHRKTDYETIVPLQLLRQAEQTKRIFNIVLGSIAAISLLVGGIGIMNIMLATVTER
                     TREIGIRRALGAKRRHITMQFLVETVVLSVGGGLVGVAVGVMVPLAVSQLTTMRTIVT
                     PWSVLLAFSISGAIGIIFGIYPARAAAQLDPIEALRHE"
     gene            4275..4640
                     /locus_tag="VIL39_02300"
     CDS             4275..4640
                     /locus_tag="VIL39_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480288.1"
                     /translation="MAILAPSQNAAFTSFSKVRGQVIKIAALVVMSLALGFGYDRAAP
                     GTYVAEREAGFFTGMMHGALMPAALPTLVFGKDLPIYARNNVGRGYNIGFILGLNACG
                     TLFFGIGFWHPKNRASTVT"
BASE COUNT          974 a         1485 c         1508 g          872 t
ORIGIN      
        1 ccgcactcgt ggattatcag cagacccggc tgcaactcct gctggacatc ggcgcgctcg
       61 acaccgaggc gcctaagttc tggcttaaag ataatctggc ggcttatcta cccggcaaaa
      121 cgcctgcccc aggcaatccg cagccgccgg attcagggaa gcagtcagtc cttcctcccg
      181 accagtactt cagtgaataa tcaactatga atgtccaaac tctttaccag gggctggttc
      241 gccgcctcaa agagcggccc cggctggccg ccggcctcgg cctcgccgtg ttggtgctcg
      301 gggtcatggt tttcgccagg cctcggccgc aaacgcaggc tgtaagctgc tacgaggtca
      361 aatccggcga catgctggtt tccgtcgtcg aaggcggcac gttggaagcc gtcagcgagg
      421 tctccatccg cagcgaggtg gaagggacgg cgcgcatcat cttcatcgtg cccgaaggga
      481 gcaacgtgaa gaaagacgac ctgctggtgg aactggattc ttcgaactcg gaggacgccg
      541 tcaacctgca gcagatcaac gtcgagaggg cccagttcgc gctggtccag gccgaacagc
      601 aactggcgat ccagaagagt gtggtggaca gcgagattca ggccgcgacg ctgaaggtgg
      661 agttttcgga gtcggacctg gagaagttca cgaatggcct ggcgctgcag accgagcgca
      721 atgcgcagat tgcgattacc aatgtgctgg agaccttgca gattgaccgg gatcgcctga
      781 actggagcga gaagctctac aagcaaggct tcgagaccaa ggcgaacctc gacaaggacc
      841 ggctgacggc ctcgcaggac cagctcaggc tcgagcaggc cgaaaaagcg ctctggatgg
      901 tggaaacctt cgacaacccc aagacgaaac gctcgctgga ggcgaccttg caggaagcca
      961 aggagaacct cgaccgcgtg aaactccagg atgagcgcaa gctggcgcaa ttcgcggcag
     1021 acgtggactc ccagaaaaag acgctggcgc tgagccagga aaagctggag cgcgataaga
     1081 agcagctcgt ggccacgaag atttatgccc cgcaggatgg cctggtcgtc tatgccggct
     1141 cgggtggggg ccattggagc aacgaatcaa tgatcgagga gggcgcggtg gtgcgcaacc
     1201 ggcaagagat tatcaagctg ccggacgtct cggcgatgaa ggtgcaggtg aagatccatg
     1261 agacgtatat caaccaggtc gcgttgggcc aaccggcgtt cgtggtgctc gattccatgc
     1321 ccgaccagcg cttctgcggc aacgtgagca aggtcgcgcc gctgcccgac agccagagcc
     1381 gctggggcaa ccccgatctc aaggtttatg ccaccgagat tctcgtcact gacaagctgc
     1441 cggacatcaa gccgggcgtg tcggctcgcg ccgaggttct catcactaac ctggtcaatg
     1501 tagtcagcgt gccgatccag gcggtgacca cgcgcaaagg caagcaggtc gttttcctcg
     1561 ccagcgctcc ccaggagccg gtgcccgtta ccgtcggcca gtacaatacc aagttcatcc
     1621 agatctgttc gggagtgcgg gatggcgacc tggtgatgct ggcgcctccg ttcgacacga
     1681 aagagaagga cctgggcggg gcgatcgtcg ccgaaggtga aacgttgccg ccgggtgtga
     1741 ccaaccaaat cgcgcgcgcc aaagcgcctg gcaaagataa gggagcaaaa gaccgcagcc
     1801 gcccgggcag tgacaacatg cggcccatcc tggtcgtggc ggatacggat cagggcgacc
     1861 ggccgcgcct cgacaacccg cgctcgccga agagcgagcg tcctggaaga acagaattca
     1921 gtgggagctc gaccaaccag gcggatctgg tgaagcggtt cgacgctgat ggcgatggca
     1981 agttgaacgc cgtcgagcgg gcggagatgg agcgttggct ggcgcgccgg cgcggcacca
     2041 acgccccgtc gaacgggccg gtgctgaact aggggcggat gcaaaccacg gagcctatca
     2101 ttcggctgga aagcctggag aagcgctacg acctcggcgg cgaggcggag gtgcgcgcgt
     2161 tgcgcggcgt gaacctggtc gttaatcccg gatcttatgt cgccatcatg gggccttcgg
     2221 gctccggcaa gtccaccatg ctcaacctca tcggctgcct ggaccgtccg acctccggcc
     2281 actatttcct gggcggcacc gacgtctcgc gcatgcccga tgacgagctg tccgaagcgc
     2341 gcggggcgcg catcggtttc attttccaat cctacaactt gattgcgcag ctcacggtga
     2401 tcgagaacat ccaggtgccg ctgctttacc agggaaagga cctgcatgct taccgcgacc
     2461 actgcgtgaa gctggcctcg atggtcgggc tcgcagagcg gctgcatcac cggcccaatc
     2521 agctctccgg cggccagcaa caacgcgtcg ccgtcgcgcg gtcactggtc aacgatccgc
     2581 tgcttatcct ggccgatgag ccgaccggca acctggattc gcgcactggc caggaggtgc
     2641 tcgaactgat tgacacgctc aacgcgcaag gcaagacgat tgtcctggtg acccatgacg
     2701 agcggatcgc cacccgcgcc caccgggtgc tgcacatgcg cgacggcctg atcgagagcg
     2761 aagtcgtcaa ccggcctgac ccatgaaccc gctgagcctc caattcatca ccaccgtgcg
     2821 gctgggtgtg aagagcctga tgctgcacaa gttgcggtcg gtgttgacga tgctgggcat
     2881 catcttcggg gtctgctccg tgatcgccat gctggcgatt ggcgaggggg cttcctacga
     2941 ggcacagcag gccatcaaga agctcggcag ccagaacatc atcatccgca gcctcaaacc
     3001 gcccgaagat gccaagatgt cgggcatggg ccgcggcatc gccctggagt acggcctgac
     3061 ttacgacgac ggctcgcgca ttaagtccac catacccggc gtcgtgcgca tcctgccggt
     3121 gcgaatcatc cgggaaaacg tgcgcttcgc ccgcaactcc gttccgtgcc agatcatcgg
     3181 caccctgccg acctataccg aaatcgtcgg gctcgacatt gtgcgcgggc ggttcctctc
     3241 ggaaacggac cagatcaacc atgacaacgt ctgtgtcctg accgccggac tggcccagcg
     3301 gttgtttcct taccaggacc cgctgaccga atcgatcaag gtgaactcgt tctattaccg
     3361 ggtggtgggg ctggtgcggg agaagaacat gcccgagcag cgcacccagg cgggcaagat
     3421 ggaaggcgag ccgctcgaca acaacgtcta tatcaccctc agcgccgcac ggtcccggtt
     3481 tggcgacgtg attgtccgcc gcactgccgg cagcctcgag gtggagaagg tcgagctgca
     3541 ccagatcacc gtccagatgc aggacgtggc ggccgtcgaa gcggcggacc cacagatcaa
     3601 aaccctgctg ggccgtttcc accggaagac cgattacgag acgattgtgc ccttgcagct
     3661 cctgcggcag gcggagcaga ccaagcgcat cttcaacatc gtgctgggat ccatcgccgc
     3721 catctcgctg ctggtcggcg gcatcggcat catgaatatc atgctggcca ccgtgaccga
     3781 gcggacgcgg gagatcggca tccgccgggc gctgggcgcc aagcgccggc atattaccat
     3841 gcaattcctg gtcgagacgg tcgtgctgtc ggtgggcggt ggactggtcg gcgtggcggt
     3901 gggggtcatg gtcccgctgg ccgtctctca gctcacgacg atgaggacca tcgtcactcc
     3961 gtggtcggtg ctgctggcct tctccatctc cggcgccatc ggcatcatct tcggcatcta
     4021 cccggccagg gcggctgccc aactcgaccc catcgaggcc ctcaggcacg agtgaaggca
     4081 gcgccgtagc cgcccagccc ctttgatagg gcgcatgtgt ccggggagtt gcggggggtg
     4141 gatatcctcc atgtttggca cggcacccgc gaagaacctg agttctgaaa tacccgcgcg
     4201 agaagttcct gcgggcgcta ttgctgtcgc tattcgcttg cacggcggcg ccgcgcctga
     4261 acatgctatg gtgcatggca attctggctc caagccaaaa cgcagcgttc acgtcgttct
     4321 ccaaggtgcg aggacaggtg atcaaaatcg cggccttggt ggtgatgagc ctggccttgg
     4381 gcttcggcta tgaccgggcc gcgcccggga cttatgtggc agagcgggaa gccggctttt
     4441 tcaccggcat gatgcatggg gccttgatgc ccgccgcctt gccgaccctg gtattcggca
     4501 aggacctccc gatctatgcc cggaacaatg tgggacgagg ctacaatatc ggcttcatct
     4561 taggccttaa tgcctgcggc acgttgtttt ttggcatcgg cttctggcac ccgaagaacc
     4621 gcgctagcac ggttacatag ttacattgtt acattgttac attgttacat gagttgcatg
     4681 agtgccttgt gccgctgccc tatgtaacca tgcaacttat gtaactcatg taactcatgc
     4741 aacttatgta accagttgcc tccccccact ccaccactcc atcgcctcag tcagtagagt
     4801 ccgccgcgat agatttcgat gacgctcagc tctcccccg
//
LOCUS       DATCGS010000118         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_308502, whole genome shotgun sequence.
ACCESSION   DATCGS010000118 DATCGS010000000
VERSION     DATCGS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308502"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VIL39_02305"
     CDS             complement(<1..1103)
                     /locus_tag="VIL39_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY5480289.1"
                     /translation="MNPRNHPCLRVARVLLVLGLALFPWAGRAQPIQVKGGFKSTEYY
                     DPPNETQMKSMLEGAGAEPLPEGRIQVTEAIYRRFLTNGEVQLSVQAPQCIYDKGQRA
                     ISSSGPLRMQIANGKFSIEGEGFLYQVTNSALLVSNRVHTVLHPELLGPQSATTRTNA
                     PAVAAPGIDIFSDQFDFSEKSGLGVYQGNVRVAGTNLASTAGRLTVLLPVLEHRLQSL
                     TAEQNVIVDYEKIHATGDQVSYSADTGLVHLTGRPTLRVEDREGSGDELVFDRTNRVL
                     QAIGHARLRMPARGMGASYFLSQSSSNSATSLPPTNQFVEILCANYELRTNLAVFRDE
                     VKVSDRLGDELRGEMSCGLMTLTFTGTNELQKM"
     gene            complement(1112..1717)
                     /locus_tag="VIL39_02310"
     CDS             complement(1112..1717)
                     /locus_tag="VIL39_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5480290.1"
                     /translation="MPRSEAENRKMRAEGRKPARDRSARDSRSVTLNAALKRIRLLLC
                     DVDGVLTDASVFIGAAREGKQFNVRDGLGLVRLRREGIKVGWISGRPSPATTRRAKEL
                     KIDFLRQGKGSKVCAVESLLVQTGFRWDEVCYVGDDIVDLGALKRAGVAVAVANGVAE
                     ARAAADYVTQAGGGHGAVREVVELILKAQNKWARIVAEYVE"
     gene            complement(1719..2534)
                     /gene="kdsA"
                     /locus_tag="VIL39_02315"
     CDS             complement(1719..2534)
                     /gene="kdsA"
                     /locus_tag="VIL39_02315"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5480291.1"
                     /translation="MLSESALWKSLNVPGRLILIAGPCVIESEKLCLQVAAALKRTCA
                     RLGVFYVFKASYDKANRTSSKSFRGPGLEEGLAVLAKVRARVGVPVLTDVHTEAQAGA
                     AARVADILQIPAFLCRQTDLIAAAVRTGRIVNLKKGQFLSPEEMGQVIGKAKSAGGKK
                     LLVTERGTTFGYHNLVADMRSIPIMRRLGFPVIFDATHSVQLPGGGGDRSSGQREFAP
                     MLARCAVAAGASGVFIETHPEPERALSDGPNMIPLAEMKALLKSLLKVHSAVQ"
     gene            complement(2594..3901)
                     /locus_tag="VIL39_02320"
     CDS             complement(2594..3901)
                     /locus_tag="VIL39_02320"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5480292.1"
                     /translation="MLHTSRQSMNTSNVLSVIMGGGQGTRLFPLTKERAKPAVPLAGK
                     YRLVDIPISNCINSGLRRIYLLTQFNSASLHKHISQSYKFDHFSGGFVEILAAEQTYT
                     DASWYQGTADAVRKNLIHFMNHDWDYLLILSGDQLYRMDFRDIVDQHRETGADITVAT
                     IPVERRAAQSLGIMQIDAERRITRFVEKPKEPGMLDSLKLPPEWHASLGIAGSEELFL
                     ASMGIYVFNREIIRRLLENTLTDFGKHVIPNAIQNLRVFSFVFQGYWEDIGTIRAFFE
                     ANLDVTNELPRFNFFDMAAPIFSRPRFLPGSKINGAQIDHAVISDGCILNRAVITHSI
                     AGLRCFVGAGTVLNRVILLGSDYYESIESILEHEKDGKPRIGIGTNCRIENTIVDKNA
                     RIGDNVVISPAGKPEKVDHPNYYIRDGIVIIPKNGVIPHGMVI"
BASE COUNT          796 a         1239 c         1238 g          809 t
ORIGIN      
        1 atcattttct gcaactcatt cgtgccggtg aaggtaagcg tcatcaggcc gcaactcatt
       61 tccccgcgca gctcatcgcc gaggcggtcg ctgactttga cctcgtcgcg aaagacggcc
      121 aggttggtgc ggagctcgta attggcacag aggatttcga cgaactggtt ggtcggcggg
      181 agtgacgttg cggagttgga actggactgt gaaaggaagt aggaggctcc catgcctcgg
      241 gcgggcattc ttagccgggc gtgaccgata gcctggagaa cccggttggt ccggtcgaaa
      301 acaagctcgt cgccactgcc ttcccggtcc tcgacccgca aggtcggccg accggtcaaa
      361 tgaacaaggc ccgtgtccgc cgagtaggat acctggtcac cagtcgcatg gattttctcg
      421 taatcaacga tgacgttttg ctcagccgtg aggctttgca gccggtgctc gagcaccggt
      481 aacaggacgg tcagccggcc tgcggtcgag gccaggttcg tcccggccac gcgaacgtta
      541 ccctggtaaa cgcccaggcc ggacttctcc gagaagtcga actggtccga gaagatgtca
      601 atgcccgggg ccgccaccgc cggcgcattg gtgcgggtgg ttgcggattg cgggccgagc
      661 aactcaggat gaagaaccgt atggactcga ttggagacca ggagagcgga attggtcacc
      721 tggtagagga atccttcgcc ttcgatggaa aacttcccat tcgcgatctg catgcggagc
      781 ggcccggagg aactgatcgc ccgctggccc ttatcataga tgcattgggg ggcttgcaca
      841 ctgagttgga cctcgccgtt tgtaaggaat ctccggtaga tggcttcagt gacctggatc
      901 cgtccctcag gcaggggttc agccccggca ccctccagca ttgatttcat ctgggtctca
      961 ttgggcgggt cgtaatactc ggtggacttg aacccgccct tgacttgaat gggctgcgcg
     1021 cgcccagccc aggggaacag cgccaggccc agcacaagca gcacccgcgc gacacgcaag
     1081 caaggatgat ttctcgggtt catcggctgg ttcattcgac gtattctgcc acgatgcgcg
     1141 cccacttgtt ctgcgctttc aagatcaatt ccaccacctc gcgcacggct ccatgtccgc
     1201 cgcctgcctg ggtgacgtaa tcagccgcgg cccgtgcttc cgccacgccg ttggccaccg
     1261 ccacggccac accggcgcgc ttcagggccc ctagatcgac gatatcatcg cccacatagc
     1321 aaacttcatc ccatcggaag ccggtttgca cgaggagact ttccacagcg cagactttac
     1381 tgccctttcc ctgccgaagg aaatctattt tcagttcctt ggccctccgt gtcgtggcag
     1441 gcgatggacg gccggagatc cagcctacct tgattccctc acgccgcagg cgcacgaggc
     1501 ccagaccgtc ccggacgttg aattgctttc cttcccgtgc cgccccgatg aagacggacg
     1561 cgtcggtcag gaccccatcc acgtcgcaga gcaacaaccg gatgcgtttg agggccgcgt
     1621 tgagagttac ggagcgggag tcgcgggcag aacggtcacg agcgggcttc cggccctcgg
     1681 ccctcatctt tcgattctcg gcttccgaac gaggcattct attgaacagc cgaatggacc
     1741 tttagcaggc ttttcagcag tgctttcatc tcggccaggg gaatcatgtt tggcccgtcg
     1801 ctcaaggccc gttccggctc ggggtgggtc tcgatgaaaa cgccgctggc ccccgccgcc
     1861 actgcgcagc gtgccagcat cggcgcaaat tcgcgctggc cgctcgatcg gtcgccaccc
     1921 ccgccgggca actggacgga gtgggtcgcg tcgaagatta ccgggaagcc aagccgacgc
     1981 atgatcggga tcgaacgcat gtccgcgacc aggttgtgat agccaaatgt cgtgccgcgc
     2041 tcggtcacga gaagcttttt gccgcccgcg ctcttcgcct tgccaatcac ctgccccatt
     2101 tcctccgggg agagaaactg tcctttcttc aggttaacga tcctgccggt ccggactgcc
     2161 gccgcgatta agtcggtttg gcggcagagg aaggccggga tttgcaggat gtctgccacg
     2221 cgagcggcgg ctccggcctg agcttcggtg tggacgtctg tgaggaccgg gacaccgacg
     2281 cgggcgcgaa ccttcgccag caccgccagc ccctcttcca gcccgggacc ccggaacgac
     2341 ttggaggagg tgcgatttgc tttgtcgtag ctggccttga agacgtagaa aacgcccaat
     2401 cgggcgcagg ttcgcttaag cgccgccgcc acctgcaggc agagtttctc gctctcgatc
     2461 acacacggcc cggcaatcag aatcagccgg cccggcacgt tcaaggattt ccaaagggca
     2521 gattcactaa gcacgccaag gttttaccag ccgaggccgt gaatgtaaat gacactgaat
     2581 gcggccccct tgctcagatc accatcccgt gcgggatcac gccgttcttg ggaatgatta
     2641 cgatgccgtc gcggatgtaa taattcggat ggtccacctt ttcaggcttg ccggcgggcg
     2701 agatgaccac gttgtcgccg atccgcgcgt tcttgtccac gatggtgttt tcgatgcggc
     2761 agttggtgcc aatgccgatg cggggtttgc cgtctttctc gtgctccagg atggactcga
     2821 ttgactcata gtagtcgctg ccgagaagaa tcacccggtt cagcaccgtt cccgcgccga
     2881 cgaagcagcg caggccggcg atgctgtggg tgatgacggc gcgattgaga atgcagccgt
     2941 ctgagatcac ggcatgatca atctgggcgc cgttgatttt cgagccgggc agaaagcgcg
     3001 ggcggctgaa gatcggcgcc gccatgtcaa agaagttgaa gcgcggcaac tcgttggtga
     3061 catccaggtt ggcctcgaag aaggcgcgaa tggttccgat gtcctcccaa tagccctgga
     3121 acacgaagga gaacacgcgc aggttctgaa tcgcgttcgg aatcacatgc ttgccgaagt
     3181 cggtcagggt attctccagc aggcggcgga tgatctcacg gttgaagacg tagatgccca
     3241 tggacgccag aaacaactcc tcactccccg caatccccag cgaggcatgc cactccggcg
     3301 gcaatttcag cgaatccagc atccccggct ccttcggctt ctcgacgaag cgcgtaatgc
     3361 ggcgctcggc gtcaatctgc atgatcccca acgactgagc cgcgcgccgt tcaacgggaa
     3421 tggtcgccac cgtgatgtca gcgcccgttt ccctgtgctg gtcaacgatg tcgcgaaaat
     3481 ccatccggta gagttggtcc ccgctcagga tgagcagata gtcccagtcg tggttcatga
     3541 agtgaatcag gttcttgcga acggcatccg ccgtgccctg ataccacgac gcgtcggtgt
     3601 aggtttgctc ggcggcgagg atttcaacga agccgcccga gaagtggtcg aacttgtaag
     3661 attgggagat gtgcttgtgc agggaggcgg agttgaactg ggtcagcaag taaatgcgcc
     3721 gcaggccgga attgatgcag ttggagatgg ggatatcgac caggcgatac ttgccggcca
     3781 acggcaccgc cggcttggcg cgttccttgg tcagcgggaa caagcgtgtg ccctgcccgc
     3841 cgcccatgat cacggaaagg acgttgctgg tattcatgga ctgccggctg gtgtgcaaca
     3901 tagtaaactt ctctaagttc tctagatcct actccaactt ataaccccca gacgacacca
     3961 gggcaagcaa catcggagca gcgaataatt gcggggcgta tcctgacacc accaccaagg
     4021 cgggcggcta tgccaatcgg aacgccggat tcatccggca gccccagtgc ggcccgccgg
     4081 cc
//
LOCUS       DATCGS010000119         6196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_357565, whole genome shotgun sequence.
ACCESSION   DATCGS010000119 DATCGS010000000
VERSION     DATCGS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357565"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VIL39_02325"
     CDS             <1..689
                     /locus_tag="VIL39_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480293.1"
                     /translation="MKIIAGQATEAERAELDALVARQPELKAELERLRADVRLAREVL
                     PLANATEATAPELPAYARGRLQTKVRETLGRPPVPGGAPEESTLGVLWKWRWVLGLAA
                     GTAVLVLLLLPVFSRSGPPMIQVAMLDTAGATRGSDTNEALLLRQTWEKAAVDSFSGT
                     EALHAWETNWPAQNRRPAVKVVFDRAAGEVRILGRWKGESFTKTLLGERDLATTLEQA
                     KSFIKQRTSQ"
     gene            819..1019
                     /locus_tag="VIL39_02330"
     CDS             819..1019
                     /locus_tag="VIL39_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480294.1"
                     /translation="MIAVEETLSKLRSLSPDRSQHVLALIEDLAELQTLENKQDAEDA
                     HAALAEPGEDLPLEQLAQELGV"
     gene            complement(1114..2445)
                     /locus_tag="VIL39_02335"
     CDS             complement(1114..2445)
                     /locus_tag="VIL39_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480295.1"
                     /translation="MCAAISTSAATNDVRLVTLDPGHFHAGLVQKFMYPQVSPIVHVY
                     APAGPDLQEHLQRVEGFNARAEQPTHWDEKVYTGPDFLDRMLQEKAGNVVVLAGNNAH
                     KTEYIDRSISAGFNVLADKPMAITPADFELLQKAFSRATAKKVLLYDIMTERYEITSI
                     LQRELTRMPNLFGTLERGTPEKPAVVMESVHHFFKEVAGKPLTRPAWFYDVRQQGESV
                     PDVGTHLVDGVQWLCFPDQALDWRKDIKVRDARRWSTKLTPEQFKRSTGLDHYPDFFK
                     QDIGPDGALEVYANGEVAYALRGVNAKVTALWKFEAPPGAGDTLYALVRGTRCQLVIK
                     QGAEQNYKPALYVQNPPPAAAAHFEQNLRLALAKLNATWPGLDLKPAGAGWEIVIPDK
                     YRVGHEQHFAQVTEKFLRYLADGNLPGWEVPNMIAKYYTSTEAYRLSHRQR"
     gene            complement(2616..4022)
                     /locus_tag="VIL39_02340"
     CDS             complement(2616..4022)
                     /locus_tag="VIL39_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480296.1"
                     /translation="MNKHLHLSRRQFLKTSLAGAAGASLMPTIIPSSVLGADAPSKKI
                     QIGQIGCGRIANEMDMPGILRQDSARIVAVCDLDSKRLGLAKNRVEKHYAGKTGSPSA
                     LVVKAYGDYRELLKNGEIDAVAISTPDHWHSEPVLAAALAGKDIYVQKPLSMTLVEGR
                     EVSDTVNARKRAFQIGSQQRSDSPWPQFRRACELVRNGRIGKVHTVQVGLPTDPSGGI
                     AKEQPVPANLNYAMWLGWTPEAPYSEDRVHPQNSLAARPGWLRIDSYCLGMITGWGAH
                     HMDIAHWGLDTELTGPIEADGHAEFPKTGLWNVHGPYHIEMKYANGATVIIDNKFENG
                     VRFEGSEGWVFVSRGSAKATASDPATGNSKAFAASSPKILKSEIGPNEIHLHKSPDQH
                     LDWLNSIRSRERAATTPEIAHRSTSACEIAWITMKLGRKVRWDPVKEVFLNDDEANKM
                     RSRPQRAPYGTEAVLKKA"
     gene            complement(4065..4847)
                     /locus_tag="VIL39_02345"
     CDS             complement(4065..4847)
                     /locus_tag="VIL39_02345"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5480297.1"
                     /translation="MQTPSRIKTFVAFLTLLACLTAVALGASPESRVLVYTKNQVGKG
                     LYVHNNIPASVTAIKKLGEENNFAVDVSEDPAVFTDQNLKRYKALIFDNTNNEVFGNE
                     EQKAAFQRYIRAGGGFVGIHSATGSMRQWPWFWSVVGGKFCRHAKMQKFTVKVKDAKD
                     PSTAHFPATFEWTDEFYYVDHIPEGLHVLLAGDLTTVEDPGKDKYPGKLYGDEFPLAW
                     RHQFDGGREWYTALGHQPEHYADPQFTKHLLGGIRWAMGESK"
     gene            complement(5051..>6196)
                     /locus_tag="VIL39_02350"
     CDS             complement(5051..>6196)
                     /locus_tag="VIL39_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480298.1"
                     /translation="STVTVALDPSPGTDVIANYNIYYGAASAMYTNVVAVGTNTTVSI
                     SNLVGGATYYFAATAVDIFGLESAYATEVSALIPTVANRPPTEIVLAPITVVALPDTR
                     LHFLVGSSLATNGQVTFSLDPGAPENAQVNPTNGLFFWNVTRADAQTTNYFTVRITDL
                     TSPSQQEVQQVVVVVQDFLELGLGATAAQAGQDVSLPVTLASSSGVTNLVFAVQWPAG
                     RFTNATLSVIAPQIASGTLQSQGTNLVVRVQTSPGQAISGSAQIAQLNFQAMAGQRSA
                     FVPLAISSVAAATADGELYQNYIPHNGEVVVVGDVPLLRGAQSSGLSRSLTLFGKVGL
                     NHELQYNTNGLSAAAWHPAMSYLQTNIAQTVVVTSSSPVIFYRLVAH"
BASE COUNT         1162 a         1764 c         2059 g         1211 t
ORIGIN      
        1 ccatgaagat catcgcgggg caggcgactg aggccgagcg cgccgagttg gacgctctcg
       61 tcgcccgcca accggagtta aaggctgaac ttgagcggct gcgggccgat gtgcgccttg
      121 cccgtgaggt gctcccgctg gccaacgcaa ctgaggcaac ggcgcccgaa ctgcccgctt
      181 acgcgcgcgg acggctgcaa accaaagtcc gggaaaccct cgggcgaccg ccggtgcctg
      241 gcggagcgcc agaggaaagc acattggggg tgttgtggaa gtggcgctgg gtgctgggcc
      301 tggctgccgg aacggcggtg ttggttttgc tactcctgcc tgttttcagc cggtccgggc
      361 cgcccatgat acaggtggcg atgctggaca cggcgggggc aactcgtggt tctgacacga
      421 acgaggcgtt gctgttgcgc caaacctggg aaaaagcggc ggttgattcc ttcagcggta
      481 cggaggcttt gcacgcctgg gaaacgaatt ggccagccca gaatagaagg ccggccgtga
      541 aggttgtgtt cgaccgggct gccggcgaag tgcgtatcct gggccgctgg aaaggagaat
      601 ccttcaccaa aacccttttg ggggagcggg acctcgcgac taccctcgaa caggctaagt
      661 ctttcattaa gcagcgcacg agccagtaaa aaccagcctc cacggcttcc caaggtcgga
      721 gttgcacggg tcagcttcca tacaggcaaa cggcagacgt tagaccctac ctccgccagc
      781 accttgacgg cccggagtgg gggtggtaat gtcgcgccat gatcgcagtg gaagaaaccc
      841 tcagcaagct gcggagcctg tctcccgacc gcagccagca cgtgctggcg ctgatcgaag
      901 acctggccga attgcagacc ctcgaaaaca agcaggatgc tgaagacgcg catgctgcct
      961 tggccgagcc gggagaggac ctccccttgg agcagttggc tcaggagttg ggggtctgat
     1021 gtaccgcgtg ctgctgcgtc ctccggcgca gagcttcctc cgcagccgag cggtcgaatg
     1081 ccgactggcg ggggatgggc aggctgttcg accttaacgc tgccggtggc tcaggcgata
     1141 ggcttccgtg ctcgtgtagt acttggcgat catgttcggc acttcccagc cgggcaggtt
     1201 gccgtccgcc aagtagcgca ggaacttctc ggtgacttgc gcgaagtgct gctcgtgtcc
     1261 cacgcggtat ttgtccggga tgacgatttc ccagccggca ccggcgggtt tcaggtccag
     1321 gccgggccag gtggcgttca gcttggccaa ggcgaggcgg aggttttgct cgaaatgcgc
     1381 cgcggcggcg gggggagggt tctggacgta gagcgcgggc ttgtagtttt gctcagcacc
     1441 ctgcttgatg acgagctggc agcgggtgcc gcgcacgagg gcgtagaggg tgtcgcccgc
     1501 gcccggcggg gcctcgaatt tccacagggc ggtcaccttg gcgttgacgc cgcgcaaggc
     1561 gtaagcgacc tcgccgttgg catagacttc gagcgcgccg tcggggccga tgtcttgctt
     1621 gaagaaatca gggtaatggt cgagcccggt ggagcgtttg aactgctcgg gagtgagctt
     1681 ggtggaccag cggcgggcgt cgcgcacttt gatgtccttg cgccagtcga gcgcctggtc
     1741 ggggaaacaa agccattgga cgccatccac caggtgcgtg ccgacgtcgg gaacggattc
     1801 gccctgctgg cggacatcat agaaccacgc gggccgggtg aggggtttgc cggcgacttc
     1861 cttgaagaag tggtggacgc tttccatgac caccgccggc ttctccggag tgcccctttc
     1921 gagcgtgccg aagaggttgg gcatgcgggt cagttcgcgc tggaggatgc tggtgatctc
     1981 gtagcgctcg gtcatgatgt cgtaaagcag gaccttcttg gcggtggcgc ggctgaaggc
     2041 cttctggagc aactcgaagt cggccggtgt gatggccatc ggcttgtcag cgaggacgtt
     2101 gaagccggct gagatggagc ggtcgatgta ttcggtcttg tgagcattgt tgccggccaa
     2161 caccacgacg ttgccggcct tctcttgcag catccggtcg aggaagtcgg ggccggtata
     2221 gaccttctcg tcccagtgcg tgggctgctc ggcgcgggcg ttgaagccct cgacgcgctg
     2281 gaggtgctcc tgcaggtccg ggccggcggg cgcatagaca tgcacgattg ggctgacttg
     2341 cgggtacatg aatttctgca ccaggcccgc gtggaagtgc ccggggtcga gggtgacgag
     2401 gcggacgtcg ttggtggcgg cggaggtgga gatggcggcg cacataaaga ggaaacagga
     2461 ggcagataaa gcccaaagcc cgaaggccga aggccgaaag aaggccgaaa gccgaaggcc
     2521 gaaggccgaa gggccagcgg cgagcggtga gggctgggga aggctgcggg atggcacttg
     2581 cattggatgg tttgggtttg gggctttgac tgggttcatg ccttcttgag gactgcttcc
     2641 gtgccgtagg gggcgcgctg cgggcgggac ctcatcttat tggcctcatc gtcgttcagg
     2701 aacacttcct tcacggggtc ccaacgcacc ttgcgcccga gcttcatcgt gatccacgca
     2761 atctcgcagg cgctcgtgga gcgatgcgcg atctccgggg tggtggccgc ccgttcgcgc
     2821 gagcggatgc tgttcagcca gtcgaggtgc tggtcagggc tcttgtgcaa gtggatctca
     2881 ttgggaccaa tctccgactt aaggatcttg gggctgctgg cagcgaaggc tttgctgttg
     2941 ccggtggcgg gatcgctggc ggtagctttg gccgagccgc ggctgacaaa gacccaaccc
     3001 tcggagcctt caaagcgcac cccattctcg aatttgttgt caatgatgac cgtggcaccg
     3061 ttggcgtatt tcatttcaat gtgatacggc ccatggacat tccacagacc cgtcttcggg
     3121 aactcggcat gtccgtccgc ctcgatgggg ccggtcaact ccgtgtcgag gccccagtga
     3181 gcaatatcca tgtggtgcgc gccccagccg gtgatcatcc cgaggcagta agaatcgatg
     3241 cgcagccagc ccggtctggc cgcgaggctg ttctgcgggt gcacccggtc ttcggagtaa
     3301 ggcgcctccg gtgtccagcc gagccacatc gcatagttca ggttggcggg aacgggctgt
     3361 tccttcgcaa tgcccccgga cgggtcggtg gggagcccaa cctgcaccgt gtgaaccttg
     3421 ccaatgcgcc cgttgcggac cagctcgcac gctcgtcgga actgcggcca gggcgaatcc
     3481 gagcgctgct ggctgccgat ctggaaggcc cgtttcctgg cgttcacggt atcgctcacc
     3541 tcgcggccct cgaccagcgt cattgaaaga ggcttttgca cgtaaatatc tttgccggcc
     3601 agcgccgcgg ccagcacggg ttcggaatgc caatgatcgg gggtgctgat ggcgacggca
     3661 tcaatctcgc cgttcttgag gagctcacga taatcgccgt aggccttgac gacgagcgcg
     3721 ctcgggctgc cggtcttgcc ggcgtaatgc ttctcgacgc ggttcttggc caaacccagc
     3781 cgtttggagt cgaggtcgca gacggccaca atgcgcgcgg agtcctgtcg gagaataccc
     3841 ggcatatcca tctcgttggc gatacgaccg cagccgatct ggccgatctg gatcttcttg
     3901 ctgggtgcgt cggcgcccag gacggacgaa gggatgatgg tgggcatcag ggatgcgcca
     3961 gcggcgccgg ccagagaggt cttgaggaat tgccgccgag aaaggtggag atgtttgttc
     4021 ataatatatt atcgtttagt tgacggtatg acttgtgggg atgttcattt gctttctccc
     4081 atcgcccagc ggatgccgcc caggagatgc ttggtaaact gcggatcggc gtaatgctcg
     4141 ggctgatggc ccagggcggt gtaccactcg cgaccgccgt cgaattgatg acgccaggcc
     4201 agtggaaatt catcgccgta tagcttgcct ggatacttat ccttgccggg gtcctcgact
     4261 gtcgtaaggt cgcccgccag cagcacgtgc aagccctcgg ggatatgatc cacgtaatag
     4321 aactcgtccg tccactcgaa cgtggcgggg aagtgggcgg tggaggggtc tttggcgtcc
     4381 ttcaccttca cagtgaactt ctgcatcttg gcgtggcggc agaacttgcc cccgaccact
     4441 gaccagaacc agggccattg acgcatagag ccggttgcgg aatggatgcc gacgaacccg
     4501 ccgccggcgc ggatgtagcg ctggaaggcg gccttctgct cctcgttgcc gaagacttca
     4561 ttgttcgtat tgtcgaagat cagggccttg taacgcttga gattctgatc ggtgaacacc
     4621 gcggggtcct ccgagacgtc cacggcgaag ttgttctcct cgccgagctt cttgatggcg
     4681 gtgacgctgg ccgggatgtt gttatggacg tagaggccct tgccgacctg gtttttcgta
     4741 tagaccagca cccggctctc cggcgacgcg ccgagggcga cggccgtcag gcaagccaga
     4801 agggtgagaa aggcgacgaa cgttttaatg cgggatggag tctgcatgag catttataac
     4861 gaaatagaca caatgattca accaacagcg ccaccggcgc agctagcgga ggtattatgt
     4921 tcatgcccgg ggaatgatca agtgaaagag agcagtgaaa cgggaactgg gcgtatctca
     4981 agcgcatctg caaccccccg ttcgagaccg agagactcac ctggctggaa gcaaactgca
     5041 ggcgtggaaa ttaatgggcc acgaggcggt agaaaatcac cggactcgac gaagtcacga
     5101 ccactgtctg ggcgatgttc gtctgcagat agctcatcgc cgggtgccac gctgccgcgg
     5161 ataacccgtt ggtgttgtac tgcaactcgt ggttcaggcc gacctttcca aacaacgtca
     5221 ggcttcgcga taaaccgctg gattgggcac ccctcagaag cggaacatcc cccacaacca
     5281 ccacctcgcc gttgtgcgga atataattct gatagagctc gccgtccgcc gtcgccgcgg
     5341 ccacgctgct aatagccagc ggcacaaacg ccgatcgctg gcccgccatc gcctggaagt
     5401 tcagttgagc aatttgtgcc gaaccgctga tagcctgtcc cggtgaggtc tgcacgcgga
     5461 ccaccaaatt cgtgccctgg ctttggagcg tgcctgacgc aatctggggt gcgatgacag
     5521 acaacgtcgc gtttgtgaaa cgtcccgcag gccactgaac cgcaaacacc aggttggtta
     5581 ctccgctgct tgaggccagc gtcacgggga ggctaacgtc ctgaccggcc tgcgcggcgg
     5641 tcgcacccag gccaagttca agaaagtcct gcacaaccac gaccacctgc tgcacctcct
     5701 gttgggatgg cgaagtcagg tccgtaatcc tgaccgtgaa gtagttcgtc gtctgggcat
     5761 ccgcccgcgt gacattccag aaaaacaatc cattggtagg attgacctga gcgttctccg
     5821 gcgcaccggg gtcgagacta aacgtcactt ggccattggt ggccagtgaa ctgccgacga
     5881 gaaaatgcaa gcgggtgtcc gggagtgcca caactgttat cggcgccaaa acaatctccg
     5941 tcggcggccg attcgccaca gtggggatga gggccgaaac ctctgtggca taagcactct
     6001 ccaggccgaa gatatcgacc gcggtggcgg caaaatagta agtggcacct ccgaccaggt
     6061 tggagatgga cacagtggtg ttggtgccaa ctgcgaccac gttggtgtac atggcactgg
     6121 cggcgccata gtaaatgtta tagttggcga ttacgtcggt gccgggactt gggtccaaag
     6181 ccaccgtaac cgtgct
//
LOCUS       DATCGS010000120         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_555732, whole genome shotgun sequence.
ACCESSION   DATCGS010000120 DATCGS010000000
VERSION     DATCGS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555732"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VIL39_02355"
     CDS             <1..363
                     /locus_tag="VIL39_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480299.1"
                     /translation="WKAVQPLVQKVMDARRDTMSGMGRGMFGRGGPGGGGNAQPADQQ
                     GQQRRGGFGGAPNPDADALQKAIDSKASKAELKTALDKYITSRKAKQATLEQAQADLR
                     KVLTFRQEAIATLNGLLN"
     gene            538..2307
                     /locus_tag="VIL39_02360"
     CDS             538..2307
                     /locus_tag="VIL39_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5480300.1"
                     /translation="MSEIVAQDMSMKPLLDKMVAAKQLSATDAAILVRQSQSGAQKPL
                     QAEADVLRWLAQEYDLTYTTLEDIDLDRQLLSLFPARLLLKEELLPLRRINGSVEVAT
                     SRLFATQGLDALKTMTGLTLKPVLASSEAIQREIKKRLGVGADTLGTLNEDESFTVVD
                     EHREEDTDLDTAAEDASIIRFVNQVLRDAIELRASDIHLEPFEEEFRIRYRIDGVLQD
                     VPVPSQIKRFQPAIVSRVKILSHLNIAEKRLPQDGRIKIRNEDAEVDIRVSVIPMLHG
                     EALVLRLLRQNSTLLGMGQLGMDTRELDCFRRVLQMPHGIILVTGPTGSGKTSTLYTA
                     LNEINDAERKIITIEDPVEYQLLGVNQIQVSEKAGLTFARGLRSILRHDPDVILIGEI
                     RDQETAQIAVQASLTGHLVFSTLHTNDAPGALTRLVDMGVEPYLVASSLEAVLAQRLV
                     RVLCERCKQPDHSPTAEAFKAQVGIPANTTIYRSVGCQECRHTGFFGRHAIFEWMDSD
                     NEIRQLILKNASSDVIRDAARKGGMKTLADDGWRLVRMGITTVEEVLSVTTAKEVERT
                     TRKQSPEAAEKEPTPASVAVSRQ"
     gene            2326..3573
                     /locus_tag="VIL39_02365"
     CDS             2326..3573
                     /locus_tag="VIL39_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5480301.1"
                     /translation="MPLFQYKALQANGAMSEGQLEAADRQGAFSQIAGLGLRPVSLSE
                     KAGAGAATGFALPAALAGLSFRKRSAKVSGRELENFTRLLSSLLAAGVPLSRALVILH
                     KESSSPAASTKWKEVHDLVVDGMALADAMGKSPETFPRVYTAMVQAGETGGFLDLVLA
                     QIADFQAREKELRSKVMAAMLYPSILFFLAIGVLIFLLTFFIPRFQGIFKGFGATLPL
                     ATQIVIGVSNIVRGYGAFVVVGLVVAGFLLRTWFSSEKGRRTWEGLVLRSPILGPLVA
                     EFAMARFCRMLGTLLGAGVPLVQGLSVARKSIGNQILVDAVSQSIERVQQGGQLGASL
                     AESRGLFPNSVVEMISVAEESGRLDQELVRIANVTEGDLDRELKTAVALMEPAMLFFI
                     AAFIGTIFISMVLPIFTLQDYIK"
     gene            3593..4039
                     /gene="gspG"
                     /locus_tag="VIL39_02370"
     CDS             3593..4039
                     /gene="gspG"
                     /locus_tag="VIL39_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY5480302.1"
                     /translation="MKLNNSDKNRTVRSRQHGFTLVELLLVLVILGILAAIVLPRFTN
                     RTKQAQIAATQTQIATFKTALDAYEVDNGYYPKGKNGLMDLIQQPRDAQNWRGPYLQA
                     DAIPKDPWFNDYIYECPGRHNPSSYDLSSLGPKDENNIIANWTIKR"
     gene            4040..>4621
                     /locus_tag="VIL39_02375"
     CDS             4040..>4621
                     /locus_tag="VIL39_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY5480303.1"
                     /translation="MRLLTGQPGSGSAARFAHQARRGFTLIELILVMALLTIVISLTA
                     PKLSRFFHGRTLDSEARRLLALTRSGQSRAVSEGMPMDLWVDAQQGAFGLQAEASYDT
                     SDPKAVDFTLDSGLQLEVVNKTVAAPVNTVTMNQAQRASTASVPRVNLVHANLPTIRF
                     LPDGSIGEASPQSLHLSSSDGGSLWLAQARDGVN"
BASE COUNT          902 a         1333 c         1544 g          842 t
ORIGIN      
        1 tggaaggcgg ttcaaccgct ggtccaaaag gtcatggacg cgcggcggga taccatgagc
       61 ggcatgggtc gtgggatgtt cggacgcggg ggcccgggcg ggggcggcaa tgcgcagcca
      121 gccgaccagc agggccagca acgtcgcggc gggttcggcg gcgcgcccaa tccggatgcc
      181 gatgcgttgc agaaggcaat tgattcaaag gcgtccaaag cggaattgaa gaccgcgctg
      241 gacaagtaca tcacctcccg caaagccaag caagcgacgc tggaacaggc gcaagccgac
      301 ctgcgcaagg ttctgacctt ccggcaggaa gccatcgcga cgctgaatgg tttgctgaac
      361 taagatgctg tggtgcgtga tgcatgacat ggggggggcg cttggcgcct cccgtgttgc
      421 gcgttgtgtg aaaccgagta gttacatgag ttacatagtt acatggttac aaaggctggt
      481 ggcgcgggtg cctcatgtaa cccatgtaac gatgtaactt tgtaaccctt accagtcatg
      541 agtgagattg ttgcccagga tatgtcaatg aagccgttgc tcgataagat ggtagcggcc
      601 aagcaactgt cggcgacgga tgcggcgata ttggtgcggc aaagccagtc gggagcacag
      661 aagcccctgc aagcggaggc ggacgtgctc cggtggctgg cgcaggaata tgatctcact
      721 tacacgacgc tggaggatat tgaccttgat cgccagcttc tctcactatt tcccgcgcgg
      781 ctcctgctga aggaggagct gctgccgctg cggcgaatca acggcagcgt ggaagtggcc
      841 acgagccgcc tgttcgccac ccaggggctg gatgcgctca agaccatgac cggtttgacc
      901 ctcaaaccgg tgctggcttc gagcgaggcc atccagcggg agatcaagaa gcggctgggc
      961 gtgggggcgg acacgctcgg caccctgaac gaggatgagt cgtttacggt ggtggatgag
     1021 caccgcgagg aagacaccga tctggacacc gcggccgagg acgcctcgat catccgcttt
     1081 gtgaaccagg tgctgcggga tgcgatcgag ctgcgggctt cggacatcca tctcgaaccg
     1141 ttcgaggagg agttcaggat tcgctaccgg atagacggcg ttttgcagga cgtgccagtg
     1201 ccatcgcaga tcaagcgatt ccagccggcc atcgtgtcgc gcgtcaagat tctcagccac
     1261 ctgaacatcg ccgagaagcg gctgccgcag gacgggcgca tcaagatccg gaacgaggac
     1321 gcggaggtgg acattcgtgt gtcggtcatc ccgatgctgc acggcgaggc cttggtgctg
     1381 cgcctgctgc ggcagaactc gacgctgctc gggatgggcc agctcggcat ggacacgcgc
     1441 gagctggatt gcttccgacg cgtgctgcaa atgccgcacg gcatcattct ggtcaccggc
     1501 ccaaccggca gcggcaagac ctcgacgctt tacacggcgc tgaacgagat caatgacgcg
     1561 gagaggaaga tcatcacgat cgaggatccg gtggagtatc aactgctggg cgtcaaccag
     1621 atccaggtat cagaaaaggc ggggctgacg ttcgcccgcg ggctgcgctc gatcctgcgt
     1681 cacgacccgg atgtgattct cattggggaa atccgcgacc aggagacggc gcagatcgcc
     1741 gtgcaggcgt cgttgacggg gcatcttgtg ttttcgacct tgcacaccaa tgacgcgccg
     1801 ggcgcgctga cccggctcgt ggatatgggg gtagagcctt acctggtggc gtcgtcgctg
     1861 gaagcggtac tggcccagcg gctggtgcgg gtgctgtgcg agcggtgcaa acagcccgac
     1921 cattcaccaa ccgccgaggc gttcaaggcg caagtgggca ttcccgccaa cacgaccatc
     1981 taccgctcgg tcggctgcca ggaatgccgc cacaccgggt tcttcggccg gcacgccatt
     2041 ttcgaatgga tggacagcga caacgagatc cggcagttga ttctcaagaa tgcctcgagc
     2101 gacgtgatcc gggacgctgc ccgaaagggc ggcatgaaga ccctggccga cgacggctgg
     2161 cggctggtgc gcatgggcat cacgaccgtc gaagaagtgc tgagcgtgac cacggccaaa
     2221 gaggttgagc gcaccacgag gaaacaatcg ccagaggccg cagagaagga acccacccct
     2281 gccagcgtgg ccgtgagccg gcagtaacat gccctatctg ccataatgcc ccttttccaa
     2341 tacaaagcat tgcaggcgaa tggcgcgatg agcgaaggcc agctcgaagc cgcggaccgc
     2401 cagggagctt tctcgcaaat cgccgggctc ggcctgcggc cagtcagtct ttcggaaaag
     2461 gccggcgccg gagcggcaac cggttttgcg ctgccggcgg cgctggctgg gttgtcgttt
     2521 cgcaagcggt cggccaaggt ttccggccgg gagctggaga acttcacgcg gctgctgtcg
     2581 agcttgctgg ccgcgggcgt gccgctgagc cgcgcgctgg tgattctcca taaggaatcc
     2641 tcctcgcctg ccgccagcac gaagtggaag gaagtccatg atctggtagt ggatggcatg
     2701 gccctggccg atgcgatggg gaagtcgccg gagacctttc cgcgggtcta tacggcgatg
     2761 gtgcaggccg gcgagacggg cgggttcctg gacctggtgc tggcccagat cgccgacttt
     2821 caggcgcgcg agaaggaact gcgctcgaag gtgatggcgg cgatgctcta tccgagcatc
     2881 cttttcttcc tcgcgattgg ggtgctgatt ttcctgctca ccttctttat tccgcgtttc
     2941 cagggcatct tcaaagggtt cggggccacg ctgcccctgg cgacccaaat cgtgatcggg
     3001 gtgagcaata tagtgcgagg ctacggggcg ttcgtcgtgg tcggcttggt ggttgcgggt
     3061 ttcctgctgc ggacgtggtt ctcgtcggaa aagggccggc ggacttggga gggcctggtg
     3121 ctgcgctcgc cgatcctggg tcccttggtg gccgagtttg ccatggcgcg gttctgccgg
     3181 atgctcggca cgctgctcgg ggcgggcgtg ccgctggtgc aagggctgag cgtggcccgc
     3241 aagtccatcg gcaaccagat tctcgtggat gcggtgtcgc agtccatcga gcgcgtgcag
     3301 cagggcggac agctcggcgc gagcctggcc gagagtcgcg gtctattccc caactccgtg
     3361 gtggaaatga tttcggtggc ggaagagagc ggccggctcg accaggagct ggtccggata
     3421 gccaacgtca ccgaaggtga cctggaccgg gaattgaaga cggcggtggc gctgatggag
     3481 ccggcgatgc tctttttcat cgccgcgttc attggcacga tcttcatcag catggtgctc
     3541 ccgatcttta ctctgcaaga ctatatcaaa tgataaactg cgattctgaa ttatgaaact
     3601 gaacaactct gacaagaacc gaactgtccg ttcacgccag catgggttta cgctggtaga
     3661 actgttgctg gtgctggtga tcctgggcat cctggcggcg atcgtgctgc cccgcttcac
     3721 gaatcgaacc aagcaggcgc agatcgcggc gacgcagacg cagattgcga cctttaaaac
     3781 ggccctggac gcctacgagg tggacaatgg ctactatccc aaaggcaaga acggactgat
     3841 ggatttgatc cagcagccgc gcgatgccca gaactggcgc gggccatatc tccaggccga
     3901 cgccatcccc aaggaccctt ggttcaatga ttacatttac gaatgcccgg gccggcacaa
     3961 cccgagttcc tacgacttga gctccttggg gccgaaggac gagaacaata ttatcgccaa
     4021 ctggactata aagcgttgaa tgaggttact caccggacaa ccaggcagcg ggagcgcagc
     4081 gcggttcgcg caccaggcgc ggcgcggctt cacgctgatt gagttgatcc tggtgatggc
     4141 cctcttgacc atcgtgatct cgctcaccgc gccgaagctc tcgcggtttt tccacggccg
     4201 gacgctcgac tcggaagcgc gccggttgct ggcactgacg cgaagcggcc agagccgcgc
     4261 ggtgtcggag gggatgccga tggatttgtg ggtggatgct cagcagggag ccttcggttt
     4321 acaagcggag gccagctacg acacgagcga cccgaaggcg gtggacttca cgctcgacag
     4381 cggcctgcaa cttgaagtgg tgaacaagac cgtcgcggcg ccggtcaaca cggtgacgat
     4441 gaaccaggcc cagcgggcca gcaccgccag cgtgccgcgc gtcaacctcg tccatgccaa
     4501 tctgccaacg atacgctttc tgccggacgg ctcgatcggg gaggccagcc cgcaatcgct
     4561 ccacctgagc agtagtgacg gcggttcgct ctggctggcg caagcccgcg acggcgtgaa
     4621 c
//
LOCUS       DATCGS010000121         4369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_160253, whole genome shotgun sequence.
ACCESSION   DATCGS010000121 DATCGS010000000
VERSION     DATCGS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160253"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1759
                     /gene="fdrA"
                     /locus_tag="VIL39_02380"
     CDS             173..1759
                     /gene="fdrA"
                     /locus_tag="VIL39_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEY5480304.1"
                     /translation="MTVAGQIKKGAYFDSVTLMRVGKELAALPGVTDAAVVMGTKSNQ
                     AILAASGLLISQFKHAGDTDLLIAVKARTNKAAQSALAAVDALLSKVTRKSAASSEVR
                     PVSLAGALNVLPEANLALISVAGRYAGELARRALESGLHVMLFSDNVPLEQEIELKQF
                     ARKRDLLVMGPDCGTAIINGVPLAFANAVNRGNIGIVAAAGTGLQEVSCIISNQGAGI
                     SQAIGTGGRDVKQEVGGIMFIEGLKALAQDEQTRVILLVSKPPHPQVLKRIEKVISTI
                     RKPVVTLFIGAATDGRGPATLEEAAFQAVALAQCEDPAAAFQRLAARDEYLSNQAQQS
                     ANRAPGSGRARGPNSPARRKYVRGLFSGGTFCAEAQILLQDMLADVYSNVPVRGVKQL
                     RNSLVSQKHTLIDLGEDEFTVGRPHPMIDYSLRNRRILEEAADPETAVILLDVVLGYG
                     ANPDPVADLAPVLRKAARKVGVVCSVTGTDQDPQNRSRVVAALLRSGALVMPSNAAAC
                     KLAGQIIQCLGRSSWARSSR"
     gene            1720..3186
                     /locus_tag="VIL39_02385"
     CDS             1720..3186
                     /locus_tag="VIL39_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEY5480305.1"
                     /translation="MPGTVKLGEEFEVMNPINQLFRSELKVINLGLASFKQALDDARV
                     PAVQVDWKPSVEVDPRLRQRVMASAAAIEPANARVMKIILGGMPHLVGLERAIDVIPG
                     MKPDLLLHAGPPITWERMCGPMRGAIIGALLYERRAGTAEEAQRLAASGAIKFAPCHE
                     HATVGPMAGVVSPSMPVFVLKNETYGNHAYCTMNEGLGKVLRYGAYSEPVIQRLKWME
                     STLYPVLKKAVAKLGKIDLKNIIAQALHMGDELHNRNRAATSIFYRTIAPAVVMTCAD
                     AATAAAVLEFINQNDHFFLNLSMPASKATLDAARGVKHSSIAVVMSRNGTDFGVQLSG
                     TGNAWFTGPAAVPDALYFPGYSKADANPDIGDSAITETNGLGGFAIAAAPAIVQFVGG
                     SSDDALRYTRQMYEITAAENNIYQIPSLNFRGTPTGIDVRKVIEKNILPFIDTGVAHK
                     EPGIGQIGAGVLNAPVEPFQKAFEGFARSLTKKGKTTR"
     gene            3183..4001
                     /locus_tag="VIL39_02390"
     CDS             3183..4001
                     /locus_tag="VIL39_02390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5480306.1"
                     /translation="MNTNQFLQFMDKVRVYDLTQPLSIHTPPWPSYMPLGIQYFKRIA
                     GAHMGQGANGQIITTSNHVGTHMDGEIHFHSSGRPIGKVPIEEWIGSGVVADISKEVG
                     DYDLYTPEMITKNVEVKKGDILIINTGYHRYAWDQKLSDELRYFVKHPGPGPGFHQWA
                     LDMKLKWIGVDCGSADHPMNTIIRNWHPARFAEAETKLKAKYGKAWDELFPPDEYYQV
                     MHLKLFPLKLVHAENVGGEIDKLSNQRAWIGAYPLRGIEMESAMCRIVAMLPPK"
BASE COUNT          866 a         1327 c         1425 g          751 t
ORIGIN      
        1 agcggcttaa cacccacgcc gcccgcaacg atagccttgc cttggcggct tagcccctaa
       61 tcgaagaatc ctgtcgtgcg ccctcgaaag cctggggcac taaaatgcct ttgcaatcag
      121 ggcggtttcg gagcagactg acgctgtcgt tgtaacacaa catcgcaatt cgatgacggt
      181 tgccggccaa atcaagaaag gcgcctattt cgattccgta acgctcatgc gcgtcgggaa
      241 ggaactcgcc gcactaccgg gcgtgaccga cgctgccgtc gtgatgggga ccaagtccaa
      301 ccaggccatc ctcgctgcca gcggcttgct gatctcccag ttcaagcacg cgggcgacac
      361 cgatctgctg attgccgtaa aagccaggac gaacaaggcg gcgcaaagcg ccctggcggc
      421 ggtcgatgcg ctgctcagca aagtcacccg gaagtccgcc gccagcagcg aggtccggcc
      481 ggtgagcctg gccggcgcgc tgaacgtcct gcccgaagcc aacctggcgc tcatctcggt
      541 ggcgggccga tatgcgggtg agctcgcgcg gcgcgccctg gaaagcggcc tgcacgtcat
      601 gctgttctcg gacaacgtgc cgctggagca ggagattgaa ctgaaacagt tcgcccgaaa
      661 gcgggatttg ctggtcatgg gcccggattg cggcacggcc atcatcaatg gtgtcccgct
      721 ggcttttgcg aatgccgtca accggggtaa tatcggcatc gttgcggcag ccggcaccgg
      781 cttgcaggag gtgagctgca tcatctccaa ccagggcgcc ggcatttccc aagccatcgg
      841 cacggggggg cgcgacgtga agcaggaggt cggcggtatc atgttcattg aggggctcaa
      901 ggcgctggcc caggacgagc agacgcgcgt gatcctgctg gtctcgaagc cgccgcatcc
      961 gcaggtgctc aaacggattg agaaggtgat cagcacaatc cgcaaaccgg tcgtcaccct
     1021 gttcatcggc gccgcgacgg acggccgggg accagccacg ctcgaagagg ccgccttcca
     1081 agcggtggcc ctggcacagt gtgaggaccc tgccgccgcg ttccaacggc tcgctgcccg
     1141 agacgaatac ctcagcaacc aggcccagca gtccgccaac cgtgctcctg gtagcggacg
     1201 ggcgcgtggg ccaaactctc ctgcccgccg aaagtatgtg cgcgggctgt tcagcggcgg
     1261 cactttctgt gccgaagcgc agatccttct ccaggacatg ctggcggacg tgtattcgaa
     1321 tgtgcccgtc cgcggcgtga agcagctccg gaactcgctg gtcagccaga agcatacgct
     1381 gattgacctg ggcgaagacg agttcacggt cggccggccg catcccatga ttgactactc
     1441 gttgaggaat cggcggattc ttgaagaggc ggccgatccc gagacagccg tgatcctgct
     1501 ggacgtggtg ctcggctacg gggcgaatcc cgacccggtg gcggatctgg cgcccgtgct
     1561 tcgcaaagcg gcgcggaagg ttggggtagt ttgttccgtc acgggaacgg accaggatcc
     1621 gcagaaccgc agccgggtgg tggcggcgct gctgaggtcg ggcgcgctcg tgatgccctc
     1681 caacgcggcg gcgtgcaagc tggcaggaca aattatccaa tgcctgggac ggtcaagctg
     1741 ggcgaggagt tcgaggtaat gaaccccatc aaccaactct tccgcagcga gctgaaggtg
     1801 atcaacctcg gcttggcgag cttcaagcag gcgctggatg atgcccgcgt gccggcggtg
     1861 caggtggact ggaagccttc ggtggaggtt gaccccagac tccggcagcg ggtaatggca
     1921 tccgcggctg ccatcgagcc ggcgaacgcg cgggtgatga agatcatcct gggcggcatg
     1981 ccccatctcg taggcctgga acgggcgatt gacgtcatcc ccggcatgaa acccgacctg
     2041 ctgctgcacg ccgggccgcc gattacctgg gagcggatgt gcgggccgat gcgcggcgcg
     2101 atcatcggcg cgctcctgta cgaacggagg gccgggaccg ccgaagaagc ccagcggctc
     2161 gcggcgtcgg gggcgatcaa gttcgctccc tgccacgagc atgcgaccgt gggacccatg
     2221 gccggcgtcg tctcgccctc aatgcccgtg ttcgtgctga agaacgagac gtatggcaac
     2281 cacgcctact gcacgatgaa cgaaggattg ggcaaggtgc tgcgctacgg cgcttacagc
     2341 gaaccggtga tccagaggct caaatggatg gagagcacgc tctaccccgt gctgaagaag
     2401 gccgtcgcca agctggggaa gattgacctg aagaacatta tcgcccaggc gctgcacatg
     2461 ggtgacgaac ttcacaaccg caaccgcgcc gccacctcga tcttctaccg gaccattgcg
     2521 cccgcggttg tgatgacgtg cgccgatgcg gcgaccgcgg cagccgtgct ggagttcatc
     2581 aaccagaacg accacttctt cctgaacctc tccatgccgg cctccaaggc gaccctggac
     2641 gccgcgcgcg gggtcaagca cagcagcatc gcggtggtga tgtcgcgcaa cggcaccgac
     2701 ttcggcgtgc agctctcggg gacgggcaac gcgtggttca ccggcccggc ggctgtgccg
     2761 gacgcgctat acttccccgg ctattcgaag gcggacgcga acccggacat cggcgacagc
     2821 gcgatcaccg agaccaatgg gctgggtgga tttgccatcg cggcggcgcc ggcgattgtc
     2881 caattcgtcg gcggcagcag cgacgacgcc ctccgctaca cgcgccagat gtatgagatc
     2941 accgccgccg aaaacaacat ctaccagata cccagcctga acttccgagg cacaccgaca
     3001 gggattgatg tccgcaaggt gatcgagaag aatatcctgc cattcattga cacgggcgtg
     3061 gcgcacaagg aaccggggat cggccagatc ggcgccggcg tgctcaacgc gcccgtggag
     3121 ccgtttcaga aggcattcga gggttttgcc aggagcttaa ccaagaaagg aaaaacaacc
     3181 agatgaatac caaccaattc ctccagttca tggacaaggt gcgggtgtac gacctgaccc
     3241 agccgctgag cattcacacg ccgccgtggc cgagctatat gccgctgggc atccagtatt
     3301 tcaagcgcat cgccggcgcg cacatgggtc agggcgccaa cggccagatc atcaccacca
     3361 gcaaccacgt cggcacgcac atggacggcg agattcactt ccattcgagc ggccggccga
     3421 ttggcaaggt gcccatcgag gaatggatcg gctccggcgt ggtagccgac atctcgaaag
     3481 aggtgggcga ctacgatctc tataccccgg agatgatcac gaagaacgtt gaggtcaaga
     3541 agggtgatat tctgatcatc aacaccggtt accaccgcta tgcctgggat cagaagctgt
     3601 cagacgagct gcgctatttc gtgaagcatc ccggcccggg cccgggcttc catcaatggg
     3661 cgctcgacat gaagctcaaa tggatcggcg tggattgcgg cagtgccgat cacccgatga
     3721 acaccatcat ccgcaactgg catccggccc gcttcgccga agcggagacg aagctgaagg
     3781 cgaaatacgg caaggcctgg gatgaactgt tcccgccgga tgagtactac caggtgatgc
     3841 atctgaagct gttcccgctg aagctcgtcc acgcggagaa tgtcggcggg gagatcgaca
     3901 agctcagcaa tcagcgggct tggattggag cctacccgct gcggggcatt gagatggagt
     3961 cggccatgtg ccgcatcgtg gcaatgctcc cacccaagta agctggaccg gggaagttct
     4021 tttcggtaac accccgcggc cagagcccac ggggccgcct gaagcaaatg gcggcacgcc
     4081 cttcaacccg gcattcggtt tctgagtttg gctgaaaatg tccaaactcc agggcagcca
     4141 gccttggtgg tggcatcagg atgcgcccgg gggttgcgcg gaaacggttc caggatgcgg
     4201 ctcccctagc tttggtgtag ccttgggata gccttggggt agccttggga tagccttggg
     4261 gtagccttgg ggtggccttg gggtggcctt ggggtggctt tggggtagcc ttggggtagc
     4321 cttggggtag tcttggggta gccttagggt ggccttggga tagccttgg
//
LOCUS       DATCGS010000122         6674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_407239, whole genome shotgun sequence.
ACCESSION   DATCGS010000122 DATCGS010000000
VERSION     DATCGS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407239"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VIL39_02395"
     CDS             complement(<1..1126)
                     /locus_tag="VIL39_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480307.1"
                     /translation="MVLAACSAKTNQQAAAPGGGRPAAEVGVVTVAPRTVGLVTELPG
                     RLEASRVAQVRARVAGILQKRLFREGSDVKAGQLLFEIDSAPYRAAAASAEASLARAL
                     ANLTQAAAQAERYQPLVEANAISKQEYVNAVAAQKQAEADVAAAKAAVQTAQINLGYA
                     SVTAPIAGRIGRTLVTEGALVGQGEATQLALVQQINPIYVNFTQSAGEVLRLRKAVES
                     GKLKRAAAADAAGVRVVLEDGTEYARVGKLLFADLTVDPTSGQIMLRAELPNPDGMLL
                     PGMYVRVRLQQAQANEVIQLPQQAVTRSGSGDSVMVVGADGKVSPRPVKVGSAQDGQW
                     IVLEGLKAGEQVMVDGFQKLQPGAPVKAVPWQPVAAVSGNG"
     gene            1326..1934
                     /locus_tag="VIL39_02400"
     CDS             1326..1934
                     /locus_tag="VIL39_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5480308.1"
                     /translation="MGQHPAEIAFADSHRRLIQAAADAFMKEGYRASMDRIAERAGVA
                     RQTVYNHFPSKKDLFSEVAHIAAEDILISLDGDGGDLRERLLGFAAALRQRVLCDEGL
                     AMFRTLSAEVPRLPALGQAFFDKGPGQTVRRLAAFIARVMEDGRLRRDDPLFAAETLM
                     GMLCGFEHTRRLLCAPGRASDAERTRVVEIVDCFLRAFAPEL"
     gene            2283..3317
                     /gene="iolC"
                     /locus_tag="VIL39_02405"
     CDS             2283..3317
                     /gene="iolC"
                     /locus_tag="VIL39_02405"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY5480309.1"
                     /translation="MKPQNTRRPRRTYDTFHMGRSGMDLYSNDVGADFVHIRSFAAYV
                     GGSPTNMSVGCRRLGLKSALLTAFGEDPVGDFVAHFLTQEGVDIRFSPRKPGRHTSAV
                     VLGIEPPDKFPLVFYRDNCADIALSIDDVLAAPIAQSRVFEFAGTNLSQEPSRSATLY
                     AAEVARKAGTAVVLDLDFRPDQWHDPRAYGAAVRSVLRCADIVMGTEDEINAAMLTDP
                     RSVRLTHSQVSDARVSGNTAEHVVSLLELGPAVVVEKLGEEGCRVHRRGNGSEDVPGF
                     PVTVQNILGAGDAFGAGFLYGYVKGWDLRKAARLGNACGAMVVTRHGCSISMPFWDEV
                     MAFADERGGL"
     gene            3342..4193
                     /locus_tag="VIL39_02410"
     CDS             3342..4193
                     /locus_tag="VIL39_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5480310.1"
                     /translation="MNNAILQRFDLDGRTVVITGGGGALCGAMADALGTMGAKVAVLD
                     ISLEKAETRAQAVTRSGGTALAFACDVLDAVQLRKSCEAIAAAWGPPDLLINGAGGND
                     PRGSTSVEFEESTQAGAKGQSFFDLDPAGFRHVFDLNFMGTFLATQIFARGMVARGKG
                     SIVNISSMSAFMPLTKVAAYSAAKAAVSNFTQWLAVHFSHAGVRVNALAPGFFMTEQL
                     RFLHIDPATGELLPRARQVIAHTPMGRYGQPDDLIGALVWLLSDASGFVTGVVVPIDG
                     GFSSYSI"
     gene            4239..5342
                     /gene="ugpC"
                     /locus_tag="VIL39_02415"
     CDS             4239..5342
                     /gene="ugpC"
                     /locus_tag="VIL39_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY5480311.1"
                     /translation="MANVILKNLVKRFNAVTAVDDLSLEIHDREFAVLVGSSGCGKTT
                     ALRMIAGLEPITSGEIFIGDTLVNDMDPKDRDIAMVFQNYALYPHMNVRDNMGFGLKI
                     RKFSGEEIEGRVREAADILGIHDLLERKPKELSGGQRQRVALGRAIVRKPKVFLFDEP
                     LSNLDAKLRVAMRAEISKLHRRLGATIIYVTHDQVEAMTMADRIFIMHKGALQQTGMP
                     MEVYAKPANLFVAGFIGSPAMNFIDAKLVREAGAWFIDAGGFKVRAPEAFNARLEPHA
                     GRTVIFGVRPEDMTAHDPAAGVGDGNTVTTQADVVETLGPEIFVHLTCGAHSLVARME
                     APELLLTEGQTLEVDLKMVKTHVFDKETSQTIV"
     gene            5379..>6674
                     /locus_tag="VIL39_02420"
     CDS             5379..>6674
                     /locus_tag="VIL39_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY5480312.1"
                     /translation="MRNVLKSVVLAVLLCAGLISASAFAEINWKQSQGTEIRFLMNKH
                     PFTTYIEPKVAEFEKLTGIKVVIEAFPEDQYRNKLTIELNSGGKVDGYMIMPGQDDLY
                     YSKAGWLQPLDAYIADPKLTDPTWDPKDFFASFTKASSVDGKQIGIVINAETSLLAYR
                     KDLFTKFKQKVPTTMKELEQVAKYFHGKEVDGKKLVGITLRGKKAAATSQWVDFLYSF
                     GGSWTNAQGQSNFASPEDIAAFKFYGDLLRLYGPQGGTMLHWAESTSSFMEGKAAMIY
                     DANVFKSLYENPKESKVAGQVGYAVIPAGPKGLRLPHVSNWSLSISKASTPERQKAAW
                     LFIQWATNKENGLGALLAGVPSGRISSWASKEYKAGDKQPDWTAASMKSFDIGQGQWN
                     PPVLNVPEIRDITGQVIVDAIEGKNVAESAKKAAELMDKK"
BASE COUNT         1261 a         2060 c         2096 g         1257 t
ORIGIN      
        1 cgccgttgcc tgagaccgcc gccacaggct gccacggcac ggctttcacc ggcgcgccgg
       61 gctgcagttt ctggaaacca tcgaccatca cctgctcgcc ggctttcagt ccctcgagca
      121 cgatccactg cccatcttgt gccgagccga ccttgaccgg gcgcggcgac actttgccgt
      181 ccgcacccac caccatcacg ctgtcgccgc tgcccgagcg tgtcactgcc tgctgcggca
      241 actgaatcac ttcgttcgcc tgcgcctgct gcagacgcac gcgcacgtac atgccgggca
      301 gcagcatgcc gtcaggattg ggcagttcgg cgcgcagcat gatctggccg gaagtgggat
      361 ccaccgtcag gtctgcgaac agcagtttgc cgacccgcgc gtactcggtg ccgtcttcca
      421 ggacgacgcg cacgccggcc gcatcggccg ccgccgcgcg cttgagcttg ccgctctcga
      481 ccgctttgcg caggcgcagc acctcgccgg ccgattgcgt gaagttcaca tagatcggat
      541 tgatctgttg caccaaggcg agttgtgtcg cctcgccttg ccccaccagc gcgccttcgg
      601 tcaccagcgt acggccgatg cgcccggcga tcggcgcggt cacgctggca tagccgaggt
      661 tgatctgcgc cgtctgcact gcagccttgg ccgccgccac atcggcttcg gcttgcttct
      721 gcgccgccac cgcgttgaca tactcctgct tgctgatggc gttggcttcg accaagggct
      781 ggtagcgctc ggcctgtgcg gcggcctgag tcaggttggc gagcgcgcgc gccagcgagg
      841 cctcggcgct tgcagccgcg gcgcggtacg gcgcgctgtc gatttcaaac aagagctggc
      901 cggcttttac gtcactgcct tcgcggaaca ggcgtttctg cagaatacct gccacccggg
      961 cgcgcacctg cgccacgcgc gaggcctcca gccggccggg caattcggtg accaggccaa
     1021 cggtgcgcgg cgcgacggtg acgactccca cttcggccgc cggcctgccg ccgcccggtg
     1081 cggccgcctg ctggttggtc ttcgcactgc aagccgccag caccatcgcc acgcaggctg
     1141 cgccgcacgc accgaagaag atggacttgg agacgaggtt tttcagtgca gtcatggatt
     1201 accttcctcg agagacgccc ccgcgtggat ggggcgtatg cattgagaga tagcgctaga
     1261 tctgagcgga tattatatac tagactgtat agtctaatat atgagcaaag gctcaaggag
     1321 ccccaatggg ccaacaccca gccgaaattg ccttcgccga cagccaccga cgcctgatcc
     1381 aggctgccgc cgacgccttc atgaaagagg gttatcgcgc cagcatggac cggattgcgg
     1441 aacgcgccgg cgtcgccaga cagaccgttt acaatcattt tcccagcaag aaagatttgt
     1501 tcagcgaagt ggcccacatc gccgccgagg acatcctgat ttccctggac ggtgacggag
     1561 gagaccttcg cgagcgcctg ttgggcttcg ccgcggcctt gcggcagcgc gttctgtgcg
     1621 atgaaggcct tgcaatgttc cgcaccctaa gcgccgaagt gccacgtctt ccggctttgg
     1681 gccaggcgtt ttttgacaaa gggcccggac agaccgtacg ccggctcgct gctttcatcg
     1741 cccgcgtcat ggaggatggt cgcctgcgcc gggacgaccc cctgtttgcc gccgagacac
     1801 tgatgggcat gctgtgcggc ttcgagcata cccgccgcct gctctgcgcg cccggtcgtg
     1861 cttcggatgc cgaacgaacc agagtcgtcg agattgtcga ttgctttctc agggccttcg
     1921 caccggaact ttagcggcac cttggcgcta gtgccaccgt tttgacctga gcgtttattc
     1981 gtcccgacaa ctccttcgtc ggcgagatgt tcacattgcc gtcgacatcc aacgcacccg
     2041 aggcaatctt gagtcgcgtg aaacggtacg gcacgcttcc tgtccttgga cgcgaagcgc
     2101 gtctccggat acggccgaag cgaatgtcgt cccaacattg ggaccttcct aaaataacga
     2161 acggcctgtc ggattgacct gcgatttgaa tgtacccagc tcatttgtca cgtcaattga
     2221 tctttatcaa accgtatgtt cgccgggcgg aatctaatat ccgaatatcg aaaaattacg
     2281 aaatgaaacc ccagaacacc cgccggcccc ggcgtactta cgacacgttt catatgggcc
     2341 gttccggcat ggacctctac agcaacgatg tcggggccga ctttgtccat atccggagct
     2401 ttgccgccta cgtgggaggc tcccccacga acatgagcgt cggttgccgc cggctggggt
     2461 tgaagtcggc gctgctgacc gccttcggag aggaccccgt cggtgatttt gtcgcccatt
     2521 ttctgaccca agaaggagtg gacatccgct tcagcccccg gaaaccgggt cggcatacga
     2581 gtgccgtcgt gctgggcatc gagccgcccg ataaattccc gctggtcttc taccgcgaca
     2641 actgcgccga tattgcccta tcgatcgatg atgttctggc cgcgccgatc gcccagagcc
     2701 gcgtgttcga gttcgccggc acgaatctca gccaggagcc gagccggagc gccaccctgt
     2761 atgcggcgga ggtcgcgcgc aaggccggca cggcggtcgt cctcgatttg gatttccggc
     2821 cggaccagtg gcacgatccc cgcgcctatg gcgcggcggt gcgctctgtg ctgcgttgcg
     2881 cggatatcgt gatgggcacc gaagacgaga tcaacgccgc catgctgacc gatccccgct
     2941 ccgtccgatt gacccactcc caagtaagcg acgcgcgcgt gagtgggaat acggcggaac
     3001 atgttgtcag cctcctggag ctgggccccg cggtggtcgt cgagaagctg ggggaggagg
     3061 gctgccgggt ccaccggcgg ggcaacgggt cggaggacgt gccaggcttc ccggtgacgg
     3121 ttcagaacat cctcggggcc ggcgatgcat tcggggcggg attcctctac gggtacgtga
     3181 agggatggga cctgcgcaag gccgccaggc tgggaaacgc ctgcggcgcg atggtggtga
     3241 cgcggcatgg ctgctccatc tccatgccct tctgggacga ggtgatggcc tttgcagacg
     3301 aacggggcgg cttgtgatga cggacttggg gtcgaggcgg catgaataac gcgattttgc
     3361 agcgtttcga tctggatgga aggacggtcg tcatcaccgg cggcgggggc gcgctgtgtg
     3421 gcgccatggc ggacgccttg gggacgatgg gtgcgaaggt ggccgttctg gacatcagcc
     3481 tggaaaaggc cgagacccgg gcgcaggcgg tcacgcggtc cggggggaca gccctcgcct
     3541 ttgcctgcga cgtcctggat gcggttcagc tgcggaagtc ctgcgaggcg atcgccgccg
     3601 cttgggggcc gcccgatctg ctgataaacg gcgctggcgg gaacgacccc cggggaagca
     3661 cttccgttga attcgaggaa tcgacgcagg cgggggcgaa ggggcagtcg ttctttgacc
     3721 tggaccccgc cggattccgc cacgtgttcg atctcaattt catggggacg tttcttgcga
     3781 cccagatctt tgcccgcgga atggtcgcca ggggcaaggg atccatagtc aacatttctt
     3841 cgatgagcgc cttcatgccg ctgacgaagg tggccgccta ttcggccgcg aaggcggcgg
     3901 taagcaactt tacgcagtgg ctggcggtgc atttttccca cgccggcgtg cgcgtgaacg
     3961 cgctggcgcc cggtttcttc atgaccgagc aattgcggtt cctgcatatc gatccggcaa
     4021 cgggcgaact gctgccgcga gcccgccagg tgatcgcgca cacgcccatg ggtcggtacg
     4081 gtcagccgga cgacctgatc ggcgcattgg tttggctgct atccgacgcc tccggcttcg
     4141 tgaccggcgt ggtggtgccc atcgatggcg ggttttcttc ctactcgata taggcatctc
     4201 cagcgcaaaa gtaacggaag agaaaagaga catccgacgt ggccaatgtg attctgaaaa
     4261 atctggtgaa acggttcaat gccgtgacgg cggtcgacga cctgtcgctg gagatccacg
     4321 atcgtgagtt cgcggtgctg gtcggttcct ccggatgcgg caagaccacg gcgctgcgga
     4381 tgatcgccgg tcttgagccg ataacgtccg gcgagatttt catcggcgat acgctcgtca
     4441 acgacatgga ccccaaggac cgggacatcg ccatggtgtt tcagaattac gccctctacc
     4501 cgcatatgaa cgtccgggac aacatgggtt tcggactcaa gatccgcaag ttttccggag
     4561 aagagatcga aggacgggtg cgggaggcgg ccgatatcct cggcatccac gatctgctgg
     4621 agcgcaaacc caaagagctc tccggagggc agcggcagcg cgtggcattg ggaagggcga
     4681 tcgtccgcaa gcccaaggtg ttcctgttcg acgagccgct ctccaatctc gatgccaagc
     4741 tgcgggtggc tatgcgcgcc gagatcagca agctccaccg gcgtctggga gccaccatca
     4801 tctacgtcac ccacgatcag gtggaagcca tgaccatggc cgaccggatc ttcatcatgc
     4861 acaaaggcgc cctgcagcag accggcatgc cgatggaggt ctacgcgaaa ccggcgaacc
     4921 tcttcgtcgc cggattcatc ggctcgccgg cgatgaattt tatcgacgcc aagctggtcc
     4981 gggaagcagg cgcctggttc atcgacgccg gcggtttcaa agtgcgggcg cccgaggcat
     5041 tcaatgcgcg gctcgaaccc cacgccggac ggacagtcat ctttggcgtg cgtcccgagg
     5101 atatgacggc gcacgaccct gccgcgggcg ttggcgacgg aaacactgtg acgacccagg
     5161 cggatgtggt cgagacactc gggccggaga tctttgtcca cttgacctgc ggggcccatt
     5221 ccctcgtcgc ccggatggag gcgcctgaac tgctgctgac cgagggacag acgctggaag
     5281 tggacctgaa gatggtgaag acccatgtct tcgacaagga aacgtcgcag accattgtgt
     5341 agcgtgttga gcaatatcat ttgacaagga gacatcgtat gagaaatgta ctgaaatctg
     5401 tcgtgttggc ggttctgctg tgcgccggac tgatatcggc gagcgctttt gccgagatca
     5461 actggaagca atcccaggga acggagatcc gctttctgat gaacaagcat ccctttacga
     5521 cctacatcga accgaaggtg gccgagttcg agaaactgac cggcatcaag gtcgtgatcg
     5581 aggccttccc ggaggatcaa taccggaaca agctgaccat cgaactcaac tccggcggca
     5641 aggtggacgg ctacatgatc atgcccggcc aggatgatct ctactactcg aaggcgggat
     5701 ggctccaacc cctggatgcg tacatcgccg accccaagct gaccgatcct acctgggatc
     5761 cgaaggactt cttcgccagc ttcaccaagg cttcctcggt cgacggcaag cagatcggca
     5821 tcgtcatcaa cgccgagacg tcgcttttgg cctatcgcaa ggacctcttc accaagttca
     5881 agcagaaggt ccccacgacc atgaaggaac tggagcaggt cgccaagtac ttccacggca
     5941 aggaggtgga cggcaaaaag ctcgtcggca tcacactgcg cgggaagaag gcggcggcca
     6001 cctcccagtg ggtcgacttc ctctacagct tcggcggttc ctggacgaac gcccagggcc
     6061 agtccaattt cgcttccccc gaggacatcg cggccttcaa attctacggc gatctgctga
     6121 gactgtacgg accgcagggc ggcaccatgc ttcattgggc ggagagcacc tccagcttca
     6181 tggaaggcaa ggcggcgatg atctatgacg ccaatgtgtt caagtcgctg tatgagaacc
     6241 cgaaggaatc caaggtggcc ggccaggtcg gctatgccgt gattccggcc ggtccaaaag
     6301 gtctacggct gccgcacgtc tccaactgga gcctctcgat cagcaaggcc agcacgcccg
     6361 agcgtcagaa ggcggcgtgg ctgttcatcc agtgggcgac caacaaggag aacggcctgg
     6421 gtgcgctgct cgccggtgtt ccgtcgggac gcatttcgtc atgggcttcc aaggaataca
     6481 aagcgggaga caagcagccg gactggaccg cagcgtcgat gaagtccttt gacatcggcc
     6541 agggtcagtg gaatccgccg gttctcaacg tgcccgagat tcgcgacatt accggtcagg
     6601 tgattgtcga tgcgatcgaa ggcaagaacg tcgccgaatc ggccaagaag gcggccgagc
     6661 tgatggacaa gaag
//
LOCUS       DATCGS010000123         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_357647, whole genome shotgun sequence.
ACCESSION   DATCGS010000123 DATCGS010000000
VERSION     DATCGS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357647"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VIL39_02425"
     CDS             <1..342
                     /locus_tag="VIL39_02425"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEY5480313.1"
                     /translation="PPIRRLADLSPHPPTYQRLQAQLAQSPWICQGSLVCRPLLRRVG
                     RRKVRKGPYYLWTCKLKGKTVCVALSRSQYLLLAQAIRNNRRTQKLLERMQALTLKTI
                     LRKVPGVRKRK"
     gene            533..>2005
                     /locus_tag="VIL39_02430"
     CDS             533..>2005
                     /locus_tag="VIL39_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEY5480314.1"
                     /translation="MNRRFLPVPALLTLGLAALLPAPAAQAQPMAPARIDARMMRQPA
                     VSATQIAFVYAGDIWLVDKGGGAATRISSPKGEESFPRFSPDGSLLAFSGDYDGNEDI
                     YVMPVTGGLPRRITHHGAPDRMLGWYPDGQTLLYATSMTSPKNRFNQLYRVSAQGGLP
                     EKLPMPYGEFGAISPDGKTLAYVPISVDFRTWKRYRGGMNPDIWLFDLQKLTARNLTH
                     SPAADSVPMWHGETLYFLSDRDENKRANIWACDLKRDEFRQVTFFKEFDVHFPSIGPA
                     DIVFENAGRLYLLDLQTEKNREVEIRVVTDRATLKPHYENVSGYIYSATVSPSGKRAL
                     FEARGDIFSAPAEHGVVLNLTRTSGIAERYPAWSPDGKWVAYFSDRTGEYELTYRSAE
                     GATGTNQVGEQTLTKLGPGFRYYPQWSPDSKKVAFIDQAMRINLFDFEARTNQVIGRQ
                     LWMHHGELSRFTVGWSADSRWIAWAQDVDNRNSALVLYDCK"
BASE COUNT          379 a          642 c          624 g          360 t
ORIGIN      
        1 cccccgatcc gccgcttggc tgacctgagc ccccaccctc cgacttatca acgcctccag
       61 gcccaactgg cgcaaagccc ctggatctgc caaggctctc tggtttgccg ccccttgctg
      121 cgccgcgtcg gccgacgcaa ggtccgcaaa ggaccctact atctgtggac gtgcaaactg
      181 aagggcaaaa cggtctgcgt cgccctctcg cgctcgcaat atctgctcct ggcccaagcc
      241 attcgcaaca accgccgcac ccaaaagctc ctggagcgca tgcaggcgct gaccctgaaa
      301 accatcttgc gcaaggtgcc cggcgtcagg aaacgcaaat gactgcgctt acgtatgtta
      361 ggcgctaact acgtgccatt cgggggtagt gtcaggatgc accagcggcg gggtgcagcc
      421 gcaggcaaag ctggcgtttc ggccggggat ggtgcaggat tgcgggactg ttatgtgccg
      481 gcgcgagccg gcgtctgtgt ttaatggcca ttgaccgata atgaaacata tcatgaaccg
      541 ccgattcttg cccgttccag ccctgctgac tcttggactc gctgcgctgc tacctgcgcc
      601 cgcagcccag gctcagccga tggcccccgc gcgcattgat gcgcggatga tgcggcagcc
      661 ggcagtgtcg gcgacccaga tcgcgttcgt ctatgccggt gacatttggc tggtggataa
      721 gggcggcggc gcggcgacgc gcatcagctc acccaagggc gaagagtcat tcccgcgctt
      781 ttcgccggat gggtcgctgc tggctttcag cggggattac gacggcaatg aggatattta
      841 cgtgatgccg gtgacgggcg gtttgccccg gcggatcacc catcacggcg cgcccgaccg
      901 catgctgggc tggtacccgg acggccagac cctcctttac gcgacctcga tgaccagccc
      961 gaagaaccgg ttcaaccagc tttaccgcgt gtccgcccag ggggggctgc cggagaagct
     1021 gccgatgcct tatggcgagt tcggggcgat ttccccggac ggcaagacac tggcttatgt
     1081 gccgatcagc gtggatttcc gcacctggaa acgttaccgc gggggcatga acccggacat
     1141 ctggctgttc gacctccaaa agcttactgc ccgtaacctg acccactcgc ccgccgctga
     1201 ctccgttccg atgtggcatg gggagacgct ctacttcctt tccgaccgcg acgaaaacaa
     1261 acgcgccaac atctgggcgt gcgacctcaa gcgcgacgag ttccggcagg tgacgttctt
     1321 caaggagttc gacgtgcact tccccagcat cgggccagcg gacatcgtgt tcgagaacgc
     1381 gggccggctc tacctgctgg acctgcagac ggagaagaat cgtgaggtgg aaatccgggt
     1441 agtaactgac cgggccacgc tcaagccgca ttacgagaac gtttcgggct acatctacag
     1501 cgccacggta tcaccgtccg gcaagcgggc gctgttcgag gcgcgcgggg acattttctc
     1561 cgcaccggcc gagcatggcg tggtgctcaa cctcacgcgc acctcgggca tcgccgagcg
     1621 ttatccggcg tggtcgccgg atggcaaatg ggtggcctac ttcagtgacc gcacggggga
     1681 atacgaactc acctaccgca gcgcggaagg cgcaacgggc acgaaccagg tcggggagca
     1741 gactctgacg aagctggggc cgggcttccg ctattacccc caatggtcgc ccgacagcaa
     1801 gaaggttgcg ttcatagacc aggcgatgcg catcaacctg ttcgacttcg aggcccggac
     1861 caaccaggtg attggccgcc aactgtggat gcatcatggt gagctgagcc ggttcacggt
     1921 ggggtggtcg gcggacagcc gctggatcgc ctgggcgcaa gacgtggaca accgcaactc
     1981 agccctcgtg ctctacgact gcaag
//
LOCUS       DATCGS010000124         4905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_160341, whole genome shotgun sequence.
ACCESSION   DATCGS010000124 DATCGS010000000
VERSION     DATCGS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160341"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIL39_02435"
     CDS             <1..361
                     /locus_tag="VIL39_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEY5480315.1"
                     /translation="GYGYNLSLSAAPTKRPVKVAQIARPADTALLADAAQVNDFQWPA
                     SPSNPILEEFYYVDAPTNYSSRSYYPNGHFRHAQKANVVFCDGHTAAEAMTPGSLDRR
                     LPSQSVGCLRREILTLP"
     gene            complement(417..1799)
                     /gene="glmM"
                     /locus_tag="VIL39_02440"
     CDS             complement(417..1799)
                     /gene="glmM"
                     /locus_tag="VIL39_02440"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5480316.1"
                     /translation="MSSPRKIFGTDGVRGTANIEPVTAETALKLGRAAGHVFKHLESQ
                     PRGRGRHKIVLGKDTRLSGYMLENALSSGILSMGVDVLFIGPLPTPGVAYVTRSLRAD
                     AGIVITASHNPYDDNGIKFFRADGYKLDDKIEHQIESLVFSGEIETIRPTADAIGKAV
                     RIDDALGRYIEYVKACFPRGMTLEGLRIVVDCAHGAAYKSTPCVLRELGAEVIVLGNQ
                     PDGKNINKDCGSMHPTLMCQKIWEHRADLGIAHDGDADRVLLCDEKGTLIDGDDIMAI
                     AGLDLLAQGKLEKRTLVSTVMSNAGLDAAITAAGGHVVCTSVGDRQVIDEMLRHGYNF
                     GGEQSGHIIFRDYCTTGDGLVAALQILRIMRARELPLSKLAQCWTRFPQMVTNILVRE
                     KRPFEELDTVGQLVAEAEAELKPQGGRVLLRYSGTEPKARLLIEGRDSATLKKWTDKI
                     SETIRKQVGG"
     gene            complement(1813..2214)
                     /locus_tag="VIL39_02445"
     CDS             complement(1813..2214)
                     /locus_tag="VIL39_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY5480317.1"
                     /translation="MIAFLRNLLLEDFWLKLFSLALAALIWFTVTFVSQKEVRTDTRV
                     FFNLPVTVLSAAEDVRNFKVSPSEVQVTVRGDGETLQNLQSKDIRVMVDLTGVMAARE
                     LRKRVEVSAPAGVTHLRVTPEDVQVVFPHDR"
     gene            complement(2211..3092)
                     /gene="cdaA"
                     /locus_tag="VIL39_02450"
     CDS             complement(2211..3092)
                     /gene="cdaA"
                     /locus_tag="VIL39_02450"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY5480318.1"
                     /translation="MMWNIAQTGWRPVLEILILTVCIFYAFRFVRGTRGAPVVTGFLV
                     VLLALVLVSYLLKLKVLQYFLGGFSAFFLLAVLVIFQPELRRMLAELGNLPLFATAHE
                     QRENIEVIIQTVERLAEVRIGALIAIEQSIQLQKAVESGIPVDCEATPEMLETIFFPN
                     NAIHDGGVIIKGDRIASAACIFPLTRRQDLNKSLGTRHRAAIGLSEETDAVVVVVSEE
                     TGAVSYAHKGQLVRGVTIEALRAFLTSVLITPDRSRNSLGWIRSWIADRAKPVPVAVV
                     KADSPATTNTNTSGSAK"
     gene            complement(3089..3931)
                     /gene="folP"
                     /locus_tag="VIL39_02455"
     CDS             complement(3089..3931)
                     /gene="folP"
                     /locus_tag="VIL39_02455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5480319.1"
                     /translation="MILSCRQFEFTFPRPALVMGIVNVTPDSFSDGGRFLDAQAAVAH
                     ALRLVEQGAEIIDVGGESTRPGALPVAEAEELRRVIPVIERLAGQVKVPVSIDTIKPG
                     VARAALDAGASIVNDVAANREDPGLWRLVAEARAGYVCMHMQGMPQTMQANPVYADVV
                     REVGEFFFERIRRLSDCGVGREHIILDPGIGFGKTVEHNLQLLAAARGFTKFERPLLV
                     GVSRKSFIGTLLGAELAARLPGALACACVAVAAGVQIIRAHDVAETVQAIRMTEAILA
                     KRRE"
     gene            complement(4037..4435)
                     /locus_tag="VIL39_02460"
     CDS             complement(4037..4435)
                     /locus_tag="VIL39_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5480320.1"
                     /translation="MSKVKKLLHTRYRVDDLERTVKFYRDVLGLVEVRRHKSPRGSEL
                     VFLKTPGSEELIELCCFPAGGPVRVQPDLTHLAFEVESLEGFSRHLAGLGLKYSDGPH
                     LSADGGGIAFIDAPEGYEIELIQMAKSGAG"
BASE COUNT          961 a         1554 c         1459 g          931 t
ORIGIN      
        1 cggctacggc tacaacctat ccctttccgc agctcccacg aagcgaccgg ttaaagtcgc
       61 gcagatcgcc cgcccggcgg acaccgcgct gctggccgat gcggcgcaag tcaacgactt
      121 ccagtggccg gcctcaccgt ccaacccgat actcgaggaa ttctattacg tggatgcgcc
      181 caccaactac tccagccgct cgtattatcc caacggccat ttccggcacg cccagaaggc
      241 aaacgttgtc ttttgcgatg gacacactgc ggcggaagcg atgacgcccg gttcgctgga
      301 ccgccggctg cccagccagt ctgtcggctg cttgcgcagg gagattctga ctctgccgta
      361 aggagccggt ggcagagaca cgctggagag gtgtgatacg tcgtgtgata cgccgcctac
      421 ccacccacct gtttcctgat cgtctcagag atcttgtcag tccacttctt cagcgtcgcg
      481 ctgtcacgcc cctcaatgag caatcgcgcc ttgggctccg tgcccgagta gcgcagcaag
      541 acgcgtccgc cctgaggctt gagctctgct tccgcctcgg cgacgagttg cccgacggta
      601 tccaattcct cgaatggccg cttttcgcgc acaaggatat tggtgaccat ttgggggaaa
      661 cgagtccagc attgcgccag tttggagagc ggcaattccc gggcccgcat gatgcgcagg
      721 atctgcaagg cggcgacgag gccatcgccg gtcgtgcagt agtcgcggaa gattatgtgc
      781 ccgctttgct cgcctccgaa gttgtaaccg tggcgcaaca tttcatcaat gacctggcgg
      841 tcgccgacac tggtgcacac gacgtgcccg cccgctgcgg taatggccgc atccaacccc
      901 gcattgctca tgaccgtgct gaccagggtt cttttctcca gctttccctg cgcgagcagg
      961 tccagcccgg caatggccat gatgtcatct ccgtcaatca atgtgccctt ctcgtcgcag
     1021 agcaaaacgc ggtcggcatc gccatcgtgc gcaatgccca ggtcggcgcg atgctcccag
     1081 attttctgac acatgagcgt ggggtgcatc gagccgcagt ctttgttgat gttcttgccg
     1141 tcgggctggt tcccgagaac gatgacttcc gcgcccaact cgcgcagcac gcagggagtc
     1201 gatttgtaag ccgccccgtg cgcgcagtcc acgacgatgc gcagcccttc cagcgtcatg
     1261 ccgcgcggga agcaagcttt gacgtattcg atgtaacggc ccaacgcgtc atcaatacgc
     1321 accgccttgc cgatcgcgtc ggcggtcggg cggatggtct cgatctcacc gctaaaaacc
     1381 aggctctcga tctggtgttc gatcttgtca tcaagcttgt aaccgtccgc gcggaagaac
     1441 ttgatgccgt tgtcatcata agggttgtgg gaggcggtga tgacaatgcc ggcgtcggcg
     1501 cgcaggctgc gggtgacgta ggcaacccct ggggtcggca gtgggccgat gaacaacacg
     1561 tccacgccca tcgaaaggat gccggaggag agggcgttct cgagcatgta acccgaaagc
     1621 cgggtgtctt tgccgagcac aatcttgtgc cggccccggc cccggggctg gctttcgaga
     1681 tgcttgaaga catggccggc ggcccggccg agcttgaggg cggtttcagc ggtgaccggt
     1741 tcgatgttgg cagtgccgcg gacgccgtct gtgccgaata tctttctggg ggagctcata
     1801 ggacaaaacc aatcagcggt cgtgcgggaa gacgacctgc acatcttccg gcgtcacacg
     1861 caggtgggtg acgcccgccg gagccgacac ctcgacacgc ttgcgcagct cgcgagcggc
     1921 catgactcca gtaagatcca ccatgacccg gatgtccttg ctctggaggt tctgcaaggt
     1981 ctcaccatca ccccgcacgg taacttggac ctcactgggg ctgaccttaa agttgcggac
     2041 gtcttccgcc gcggacagga cggtgaccgg gagattgaag aacacgcgcg tatcggtccg
     2101 cacctccttt tggctcacaa aggtgacggt gaaccagatc agagctgcca gcgccagcga
     2161 gaagagcttc agccagaaat cttccaagag gagattgcgg agaaaagcga tcatttagcg
     2221 ctgccgctgg tattggtatt agtcgtggct ggggaatccg ctttgaccac cgccacgggg
     2281 accggcttgg cccggtcggc gatccacgac cggatccagc ccagcgagtt gcgggaacgg
     2341 tctggagtga tgaggactga agtcagaaac gcgcgcaatg cctcgatcgt gacgccgcgc
     2401 accaactggc ccttgtgagc ataggagacg gcgccggtct cctctgagac caccaccact
     2461 accgcgtcag tctcctcgct caagccgatg gcggcgcggt gccgcgtgcc cagggatttg
     2521 ttcaggtcct gccgccgggt gaggggaaag atgcaagcgg cggaggcgat acggtcacct
     2581 ttgatgataa cccccccgtc gtggatggcg ttgttgggga agaagatagt ctccagcatt
     2641 tcgggagtgg cttcgcaatc aacgggaatg ccggactcaa cggccttctg gagctgaata
     2701 gactgctcga tagcgatgag cgcgccgatg cgcacttcgg ccaggcgctc gacggtttgg
     2761 atgatgactt cgatattctc gcgctgctca tgcgcggtgg caaagagcgg caggttgccc
     2821 aattccgcca gcatccggcg cagctccggc tggaagatca ccaggaccgc caaaaggaag
     2881 aaagccgaga acccccccag gaagtattgg agcaccttga gtttcaacag atagctcacc
     2941 agcaccagcg ccagcaacac cacgagaaac cccgtcacca ccggcgcgcc gcgcgtgccg
     3001 cgcacgaacc gaaaggcata aaagatgcag accgtgagga ttagaatctc caacacgggg
     3061 cgccagccgg tctgtgcaat attccacatc attctcttcg tttcgccagg atcgcttcgg
     3121 tcatacgaat tgcctgcacg gtctccgcga catcatgggc gcgtatgatc tgcaccccgg
     3181 cggctacagc cacgcaggcg caggccagcg cccccgggag ccgcgccgcc agttccgcgc
     3241 ccagcaatgt cccgatgaag gacttgcgcg aaacacctac cagcagcggg cgctcaaact
     3301 ttgtaaaacc ccgcgccgcg gcgagcaact gcaaattgtg ctcgaccgtc tttccaaagc
     3361 cgatcccggg atcgagtata atatgctccc ggcccactcc gcaatcgctc aatcgccgga
     3421 tgcgctcgaa aaagaactcc ccgacctcgc gcaccacgtc ggcgtaaacc ggattggcct
     3481 gcatggtttg gggcatgcct tgcatgtgca tgcacacgta gccggcacgg gcctcggcca
     3541 cgagccgcca cagtccgggg tcctcgcgat tggcggccac gtcgttcacg atgctggccc
     3601 ccgcgtccag tgcggcgcgc gcgacgccgg gtttgatggt gtcaatggag accggcactt
     3661 tgacctgccc agccaatcgc tcgatcaccg ggatcacgcg gcgcagttct tcagcttcgg
     3721 ccaccggcaa agcgcccgga cgggtggatt ctccgccaac atcaataatc tccgcgccct
     3781 gctcgaccag tcgcagcgcg tgcgccaccg ctgcctgcgc gtccagaaac cgcccgccgt
     3841 cggagaacga gtcgggagtg acgttcacga tgcccatcac cagcgccggg cgcgggaagg
     3901 tgaactcaaa ttgacgacag ctcaggatca tgaaaagcaa atccgaaacc cgagagcgcc
     3961 gaacagacag cccgcccttt cggatttcgc tcctcggact tccttcggat ttcgggcttc
     4021 ggcattcgga ttttcactag cccgcgccgg acttggccat ctgtatcaat tcgatctcat
     4081 atccctccgg ggcgtcgatg aaggcgatgc cgccaccgtc cgcgctgaga tgaggaccgt
     4141 cggaatattt caagcccagc ccggccaggt gccggctgaa gccctcgagg ctttccacct
     4201 caaaggcaag atgggtcagg tccggctgca ctcgcaccgg gccgccggcg ggaaaacagc
     4261 aaagctcgat tagctcctcg ctgcccggag tcttcaggaa caccaactcc gaaccgcgcg
     4321 gtgacttgtg ccggcgcacc tccaccaggc ccagcacgtc tcgatagaac ttcaccgtcc
     4381 gttcgagatc atccacccga taacgcgtgt gcaggagctt cttaaccttg ctcatgcgtt
     4441 caccataacg cggaattgct tcaacgcaat cggcaattct gtctcctggc tccagtgggt
     4501 actttgacac gcttacgttt atggcttgtg tcagagtcca agcaaaccgc cgcgaagccg
     4561 gccacttggg aggttttgat gtgcgttaac gttaccgcac tgaatacaag tcctgcaacc
     4621 agtgaaaacg cggtcgaatc tgcgcttttt acgtctaacc accgagtctc cggggtgggg
     4681 atggaaggcg gcgagccacc gctcccgctg ttgagtatgg atgaaaaccg ccgcgacagc
     4741 cgccaaccag atcccaacat cccgatttca ttgaaccgtc tgcccaggcg tccctctggc
     4801 tttggggtag ccttggggta gccttggggt agccttgggg tagccttggg ggtagccttg
     4861 gggtggcctt ggggtggcct tggggtggcc ttgaggtagc cttgg
//
LOCUS       DATCGS010000125         6924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_605208, whole genome shotgun sequence.
ACCESSION   DATCGS010000125 DATCGS010000000
VERSION     DATCGS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605208"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VIL39_02465"
     CDS             complement(<1..455)
                     /locus_tag="VIL39_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HEY5480321.1"
                     /translation="MRKILNKLNVRMVILALGAAALIAGSALAASQRDTAKPKPSPVA
                     PKVELDETPLPRAAGAYTSFAPVVKKVAPAVVKIVTTSKAANISAPEGFGLNDPFWRH
                     FFGDQSGRQMPNRQFNAPRQHGLGSGVVVTKDGYILTNNHVVDSAEEVKV"
     gene            595..969
                     /locus_tag="VIL39_02470"
     CDS             595..969
                     /locus_tag="VIL39_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480322.1"
                     /translation="MENNEIIGWIPWPVAAATAAWFVLMAYKAGKNRVLWAFGGGMLG
                     LLVTTLVLGLGQATFLPFHSAEVASFRIKVAVLVILLVSGIGWLFTGSLHRHLLASRK
                     QTPAPVPEPAVKPPSATTKPQL"
     gene            complement(989..2278)
                     /locus_tag="VIL39_02475"
     CDS             complement(989..2278)
                     /locus_tag="VIL39_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5480323.1"
                     /translation="MKILLVKPRWFVKDGVYRFLTHVSFQPLHLGILAALSEGHDVKV
                     VDQDWDEVPFAEDFDLVGLTVTTFSSRRAFDIADVFRGKGVKVVIGGVHACLMPDECL
                     QHADAIVIGEAEYAWPLLLKDAAAGALKKTYQQENPTDMDDVPIPRRDLLRQNSLVGM
                     IQATRGCNHACKFCYLPNVPWHRHRRRSPDKVYEELKGMKQSVIFFVDDNLFVDEEYA
                     IALCEKIAPLKKAWSVQAPTTIAKNPRLLQAMQKSGCFFVQVGFQTVHPGSLQRAGVV
                     QNKIENYQEVVQRFHEHRILVQGFFVFGFDSEDRQIFKTAETHIKQMGLEDALLYILT
                     PYPGTPIYDQLKSEGRLLLEDRDKYGWANAVFKPANMTAQELEQGVQETYENLFHFFK
                     RRAPKQILKWLPFFIRHPRILVLVWQALHRRVKIVKD"
     gene            complement(2281..3474)
                     /locus_tag="VIL39_02480"
     CDS             complement(2281..3474)
                     /locus_tag="VIL39_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5480324.1"
                     /translation="MKRPGFFLCYFETTRQCDQGCPNCMTGRATPPARAELSTDEAKR
                     LVLDELKTICPKGAVSFSGGEILQRADHLELIAHNAKNGLHTFVNTSGSGLDSARLRG
                     LKSAAAGRLTLGFSLDSVDDDVQKKCRSGNRERLERLLSLCDENAVPYFVLVTISNQN
                     LESLKSTMSWLLAKKIAVIRSPFVPRGSAAKARHLCFNRNDMGSKIHPALRDNPLSYV
                     SHTPFFAAPDVTHLTLGGRGLNLGNLGCQAGRTFIGINAEGDVAPCVHLLDTPVCCGN
                     VREQPLSRIIAESEIMRTLRGERPAKGKCGRCRYGDSCRGCRSLAHYLTGDYLAEDPT
                     CFFDPESIETRSPYEETQTANARVFLSHLVQNPPWKDIFGARGRLGVNLLNLKARLNA
                     LLGNR"
     gene            complement(3471..3638)
                     /locus_tag="VIL39_02485"
     CDS             complement(3471..3638)
                     /locus_tag="VIL39_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480325.1"
                     /translation="MTRFHQCAGSLTHYSLPSQNLKPGHSRLPGLEADGKLLVNEFIK
                     ATSAPPLPARR"
     gene            3767..5122
                     /locus_tag="VIL39_02490"
     CDS             3767..5122
                     /locus_tag="VIL39_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEY5480326.1"
                     /translation="MVQQQTLNREASYSGIGLHSGSRVTMTFLPAPPNSGIRFRRVDL
                     EGKPEIEARVENVVENNRSTTLAKGNTRIHTVEHVLATFAGCGIDNVIVELDANEPPI
                     GDGSAREYCKMVQSAGIVPQDERREPYTVTAPIELEMGETVMTLFPDEAFKISCTSAD
                     KQGRFTQFYSVELSPTTWEHDLAHARTFCFYEEIEYLINNGLIKGGSLENAVVIRDDA
                     VLTTEPLRYPDEFVRHKILDIIGDLSLLGRPLRGHLMAVKPGHAANCELVRRITAQMR
                     KPLVAAQAFAPPPAAHKTAPVPEGASDATKPQDGAMDINGVMKLLPHRYPFLLVDRVA
                     KIDGNHIIGIKNITINEPYFQGHFPGHPVMPGVLQLEAMAQVAGIVMLKTAENSGKIA
                     YFMSAEDVKWRKPVLPGDVLEINVELTKMRGKIGKAKGVCKVGGEVVSEAAVTFMLVD
                     A"
     gene            5184..5951
                     /gene="lpxA"
                     /locus_tag="VIL39_02495"
     CDS             5184..5951
                     /gene="lpxA"
                     /locus_tag="VIL39_02495"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY5480327.1"
                     /translation="MVHSTAVIHPKAQIGAGCEIGPYCIVGEHVELGPGCRLHAHVVI
                     DGHTRLGQGNEIFPFASIGLKTQDLKWKGGLTRTEIGDNNTFREYVTIHSATGDGEAT
                     MVGSNNHILAYAHIAHNVRLGSHIIMSNVATLAGHVTVEDHAVVGGLAAIHQFCRIGT
                     MSMIAGCAKIVQDVPPYMIVDGNPAETRAHNKVGMERNGVSEQAQVAMRHAFKILIRD
                     GLTIPNALARIEKELPPLPEVQHLVQFVRSSERGITK"
     gene            5998..6702
                     /gene="rsmI"
                     /locus_tag="VIL39_02500"
     CDS             5998..6702
                     /gene="rsmI"
                     /locus_tag="VIL39_02500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5480328.1"
                     /translation="MNETLPPLTPGTLYLVATPIGNLEDITLRALRTLKECDVVAAED
                     TRRTGQLLKHFGISKPMLSYFQFNEAKRSEEIIGRLQRGEKVALVTDAGSPGISDPGE
                     RVVKAAVAAGFRVESVPGPSALVAALTASGLPTDEFHFIGFLPHKSGQRRKQLERLQG
                     YEGTLVLYESPYRVERLLGELSELFPTRQVALARELTKRFEEYLRGTPAELLEVLKSR
                     SLKGEFVVLVAGEPGR"
     gene            complement(6718..>6924)
                     /locus_tag="VIL39_02505"
     CDS             complement(6718..>6924)
                     /locus_tag="VIL39_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480329.1"
                     /translation="LRKVTPSNPRSRAKKQIPASSFNTRIKSPDWHAVTLRETEQLVR
                     EGKAEFSDWQTARCRVRRKAARMA"
BASE COUNT         1394 a         1916 c         2281 g         1333 t
ORIGIN      
        1 actttcactt cttcggcgct atccacgacg tggttgttgg tgagaatgta gccgtctttg
       61 gttacgacga cgccagagcc gaggccgtgc tggcgcgggg cgttgaactg gcggttgggc
      121 atctgacggc cagactggtc accaaagaag tggcgccaga aagggtcatt gagaccaaac
      181 ccctccgggg ccgagatgtt tgccgccttg gacgtggtga ctattttcac gacggcaggg
      241 gcgaccttct tcacgacggg cgcgaagctg gtgtaggcgc cggccgcgcg cggaagggga
      301 gtttcgtcca actcgacctt gggagcgacg ggcgaaggtt tgggtttggc tgtatctctc
      361 tgactggctg ccagggcgga gcccgctatc aatgccgccg cgcctaacgc cagaatcacc
      421 atccgaacat tcaatttgtt tagtattttt ctcataagta ttgcgttgaa cttgttttcg
      481 tttatacgga ttagagcgat atgaccgtga caagaagctt tcccgggcat tacagttctg
      541 taaggaacga ttgggcgtga tgaacggtcg cgcatggatt agagtcaaac gagcatggaa
      601 aataacgaga ttatcggttg gataccctgg ccggtggcgg cggcaacggc agcgtggttt
      661 gtcctgatgg cttacaaagc cggcaagaat cgggttctgt gggcgttcgg agggggaatg
      721 ttggggctgt tggtgacgac ccttgtactg ggcctggggc aggccacctt cctaccgttc
      781 cacagtgctg aagttgcctc gttccgcatt aaagtcgcgg tgctggtgat cctgctggtg
      841 tctggcatcg ggtggctttt cacgggaagc ctgcaccgtc acctgctggc gtccaggaaa
      901 caaaccccag cgccagtccc ggaaccggcg gtcaaaccgc cctcagccac caccaagccg
      961 cagctctaga catcctgagc cgcatcctct aatccttgac gatcttgacc cggcggtgga
     1021 gggcttgcca gacgagcacg agaatgcgcg gatggcggat gaagaaaggc agccacttca
     1081 ggatctgctt gggcgcgcgc cgcttgaaga agtggaagag gttttcgtaa gtctcctgca
     1141 cgccttgttc cagctcttgg gctgtcatgt tggccggctt gaacaccgcg ttggcccacc
     1201 catatttgtc ccggtcctcc agcagaaggc gtccctcgct cttcaactgg tcgtagatcg
     1261 gggtgcccgg gtaaggcgtc aggatgtaga gcagagcgtc ttccagcccc atctgcttga
     1321 tatgggtctc cgcggtcttg aagatctgcc ggtcttcgct gtcaaagccg aaaacgaaga
     1381 acccctgcac cagaatgcga tgctcgtgaa aacgctgcac tacctcctgg tagttctcga
     1441 tcttgttctg caccacgccg gcccgctgta aggagcccgg atgcacggtc tgaaagccga
     1501 cttgcacgaa gaagcagccg gacttctgca tggcctggag cagcctgggg tttttggcga
     1561 tggtggtggg cgcctgcacg gaccaggctt tcttcaaagg ggcgatcttt tcgcacaggg
     1621 cgatggcgta ttcctcgtcc acgaacaggt tgtcatccac gaaaaagata acgctctgct
     1681 tcatgccctt gagttcctca tagaccttgt ccggcgagcg gcggcggtgc cggtgccagg
     1741 gcacgttggg cagatagcag aacttgcagg cgtgattgca accgcgggtg gcctggatca
     1801 tgccgaccag ggagttctgc cggagcaaat cgcgccgcgg gatgggcaca tcatccatgt
     1861 cggtggggtt ctcctgctgg taagtctttt tgagggcgcc cgcagcggca tccttgagca
     1921 acaggggcca cgcgtattcc gcctcgccga tgacgatcgc atcggcgtgc tgcaaacatt
     1981 cgtccggcat caggcaggca tggaccccgc cgatgaccac cttgacgcct ttccctcgga
     2041 acacatccgc gatgtcaaaa gccctgcgcg aggaaaacgt ggtgacggtg aggccgacca
     2101 gatcgaagtc ctcagcaaaa gggacttcat cccaatcctg gtcaaccacc ttcacgtcgt
     2161 gcccctccga gagcgcggcc agaatgccca gatgcagggg ctgaaaagac acgtgggtga
     2221 ggaagcgata caccccgtct ttgacgaacc atcttggctt gaccagcagg atcttcattc
     2281 ttaacggttg cccagaaggg cgttgagccg ggctttgaga ttgagcaggt tgacacccag
     2341 ccggccccgc gcgccgaaaa tgtctttcca gggagggttc tgcaccaggt ggctcagaaa
     2401 gacgcgcgcg ttggccgtct gtgtttcttc ataagggctg cgagtctcga tgctctcagg
     2461 gtcgaaaaag caggtcgggt cttcggccag atagtcgccg gtaaggtaat gcgccaggga
     2521 gcggcagccg cggcaggagt cgccgtagcg gcagcgtccg cacttgcctt tggccggccg
     2581 ctcgccgcgc agggtgcgca tgatttcgga ctcggcgatg atccgggaca agggctgctc
     2641 gcgcacgttg ccgcagcaga ccggggtgtc cagcaggtgc acgcagggag ccacgtcacc
     2701 ctccgcgttg atgccgatga aggtgcggcc ggcctggcag ccgaggttgc ccaggttgag
     2761 cccacggccg cccagggtca ggtgggtcac atccggcgcg gcaaaaaacg gcgtatgact
     2821 gacatagctc aaggggttat cacgcagggc cggatggatc ttgctgccca tgtcgttccg
     2881 gttaaagcac agatgccggg ctttggccgc gctgccgcgc ggcacaaagg gcgagcgaat
     2941 cacggcgatc ttcttcgcga gcagccagct catggtgctc tttagcgatt cgaggttctg
     3001 gttggaaatc gtaaccagca cgaaataggg aaccgcattt tcgtcgcaga ggctcagcag
     3061 gcgctccagc cgctcgcggt tgccggagcg gcatttcttc tggacgtcat catccacgga
     3121 atccagggag aagcccaggg tcagccgccc ggcggccgcg ctcttgagcc ccctgagcct
     3181 ggccgaatcg aggccgctgc cgctggtgtt tacgaaagtg tgcaggccat tcttggcgtt
     3241 gtgcgcgatg agctccagat gatcggctct ttgcaggatt tcgccgcctg aaaaggagac
     3301 cgcacccttg ggacagatgg tcttcagctc atcgagcacc aggcgcttgg cttcgtccgt
     3361 ggagagctcc gcccgggcag gtggcgtcgc ccgcccggtc atgcaattgg ggcaaccctg
     3421 gtcgcattgc cgggtggtct cgaaatagca taagaagaaa ccgggccttt tcatcgtctt
     3481 gccggaagcg gcggagcgga ggtggctttg atgaactcgt taaccagcag tttgccgtcg
     3541 gcctcgagcc ccggcaggcg cgaatggccg ggcttgaggt tttggctcgg caaggaatag
     3601 tgcgtcaggc tgccggcgca ctgatggaaa cgggtcaagc atgctggggc tattctacca
     3661 gccggagaac aaacggcaag ggaaagacag aagtggcctt gggtgacagc gggcggcaaa
     3721 tgatctaccg cttgccaaga gggcggacgg cttgtttaat tgggtcgtgg tacaacagca
     3781 aacacttaat cgcgaagcga gctattccgg gatcgggttg catagcggca gccgggtgac
     3841 gatgaccttt ctaccggccc cgccgaacag cggcatccgg ttccgccggg tggacttgga
     3901 aggcaaaccg gagatcgagg cgcgggtgga gaacgtggtg gagaacaatc gctcgacgac
     3961 actcgccaaa ggcaacacgc gcattcacac ggtggagcac gtgttggcga cgtttgccgg
     4021 gtgcggcatt gacaacgtca tcgtggaact cgacgccaac gagccgccca tcggggacgg
     4081 cagcgcgcgg gagtattgca agatggtcca aagcgccggg atcgtccccc aggacgaacg
     4141 ccgggaaccc tacaccgtga cggcgccgat cgaactggaa atgggcgaga cggtgatgac
     4201 gctcttcccg gatgaggctt tcaagatctc ctgcaccagc gcggataagc aggggcggtt
     4261 tacgcagttt tatagcgtgg aactgtcgcc tacgacctgg gagcacgacc tggcgcacgc
     4321 ccgcacgttt tgcttctacg aggagatcga atacctgatc aacaacggct tgatcaaagg
     4381 cggcagcttg gagaacgcag tggtgattcg cgacgatgcg gtgctgacga cggagccgct
     4441 gcgctacccc gacgaatttg tccggcacaa gattctcgac atcatagggg atctatcgct
     4501 gctgggccgg ccgctgcggg gacacctgat ggcggtcaag cccggccatg ccgccaattg
     4561 cgagctggtc cggcggatca cggcgcagat gcgcaagccg ctggtggccg cgcaggcctt
     4621 cgccccgccg ccggcggccc acaaaaccgc gccggtgccg gaaggcgcgt ctgatgcaac
     4681 aaagccgcag gacggggcca tggacatcaa tggcgtgatg aagttgctgc cgcatcgcta
     4741 tccgtttctg ctggtggacc gcgtcgcgaa gattgacggc aaccacatta tcggcatcaa
     4801 gaacatcacg atcaacgagc cgtatttcca ggggcatttc cccggacatc cggtgatgcc
     4861 gggggtgctg caactggagg ccatggcgca ggtggccggc attgtgatgc tcaagaccgc
     4921 cgagaactca ggcaagatcg cctacttcat gtcagcggag gatgtgaagt ggcgcaagcc
     4981 ggtgctccca ggcgatgtgc tggagatcaa tgtggaactg acgaagatgc gcggcaagat
     5041 cggcaaggcc aaaggggtgt gcaaagtggg gggagaagtg gtcagcgagg cggccgtgac
     5101 cttcatgctg gtggacgcat agggattggc gggtgatgtt atgagggcaa tgagtggcag
     5161 gtagcttgac acaggaaaac gatatggtgc attcaacagc ggttatccat cccaaggcgc
     5221 aaatcggcgc tggttgcgag atagggccgt attgcatcgt cggggagcat gtggaactgg
     5281 ggccgggttg ccggctgcat gcgcacgtgg tgattgacgg ccacactcga ctggggcagg
     5341 ggaatgagat ctttcccttc gccagcatag gactcaaaac ccaggatttg aaatggaagg
     5401 gcggcctgac gcggacggag ataggcgata ataatacctt ccgcgaatac gtgaccatcc
     5461 atagcgcgac gggcgatggc gaggcgacga tggtggggtc gaacaatcac atcctggctt
     5521 acgcgcacat tgcgcataat gtgcggctgg gcagccacat catcatgtcc aacgtggcga
     5581 cactggcggg gcacgtgacg gtggaggatc acgcggtggt cggtggtttg gcggcgatac
     5641 atcagttctg ccggattgga accatgtcaa tgattgctgg ctgtgccaaa atcgttcagg
     5701 acgtgccccc ttacatgatc gtggacggca atccggcaga gacccgggca cacaacaagg
     5761 tgggaatgga acggaacggc gtatccgagc aagcccaggt ggcgatgcgc catgccttca
     5821 agattcttat ccgggacggt ctgacgatcc cgaacgcgct agcccggatc gagaaggagc
     5881 tgccaccgct gccggaggtt cagcatctcg ttcagttcgt ccggtcgagt gaacggggca
     5941 ttacgaaata ggaagtccaa agcccagagt ccaaaggcca aagtctgaaa tgggacgatg
     6001 aacgaaacct tgccgccgct cacgccgggg acgctctacc tggtcgcgac gccgattggg
     6061 aacctggagg atatcacgct gcgggcgctg cgcacgctca aggaatgcga cgtggtggcc
     6121 gcggaagaca cgcggcggac ggggcagttg ctgaagcact tcggcatctc gaagccgatg
     6181 ctgagctatt tccagttcaa tgaggcgaaa cgcagtgagg aaatcatcgg gcgcttgcag
     6241 cgcggggaga aggtcgcact ggtgaccgac gccggcagcc cgggcatcag cgatccgggc
     6301 gagcgggtgg tgaaggcagc ggtcgcggcg ggctttcgcg tggagtcggt gccggggcca
     6361 tcggcgctgg tggcggcgtt gactgccagc gggctgccga ccgacgaatt tcatttcatt
     6421 gggttcctgc cgcacaaatc ggggcaacgc cgcaagcaac tggagcggct tcaaggttat
     6481 gaaggaacgc tggtgttata cgagtcaccg tatcgcgttg aacggctgtt gggcgagttg
     6541 agcgagctgt tccccacgcg ccaggtggcg ctggcgcggg agttgaccaa gaggtttgaa
     6601 gagtatttgc gcgggacgcc ggcggaactg ctggaggtgc ttaagagccg ctcgctaaag
     6661 ggcgaatttg ttgtcctggt cgcgggcgag cctggccgat agcgtctcgc cggagcttca
     6721 tgccatcctg gcagccttgc ggcgaactct gcacctggcg gtttgccaat ccgaaaattc
     6781 agccttgcct tcacggacaa gctgctccgt ttcgcgcaac gtcaccgcat gccagtccgg
     6841 cgacttaatc cgggtgttaa acgaacttgc cgggatttgc tttttagccc gacttcgcgg
     6901 attggagggg gtgaccttgc gtaa
//
LOCUS       DATCGS010000126         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_506814, whole genome shotgun sequence.
ACCESSION   DATCGS010000126 DATCGS010000000
VERSION     DATCGS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506814"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1366)
                     /locus_tag="VIL39_02510"
     CDS             complement(107..1366)
                     /locus_tag="VIL39_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5480330.1"
                     /translation="MNLPPPTKRQARVIWLALTGLAVAVLVGLVVALVWGLGQVLRML
                     SPVLWPIAVAGVIAYLLDPVVDFIERKGASRPRAILCVFALALVIVAALFGSVVPQLI
                     NETRQLAVRIPAYAAHVEQRMEHWVSHPPALVKRLLERESGAKESRDSLTATNESAPA
                     FPTNSPTAAAIESTNAPLFLGGTLDNESLQTATKWLAGALPKVGYWLFGQFGRVASWL
                     GFVAGLALVPVYAFYFLLEKRGISSRWTDYLPVADSGFKNELVFILNSVNNYLISFFR
                     GQVLVAICDGILYGTGFLIIGLPYAVLIGAMAVVLTMIPYLGAIVTCCTALVIAIVQF
                     GDWQHPLLVLAVFGFVQALEGAFISPKIMGGRVGLHPVTIIIAVMAGTTLLGGLLGGI
                     LAIPLTAALRVLMFRYVWKRAGAGRDA"
     gene            complement(1373..2188)
                     /gene="proC"
                     /locus_tag="VIL39_02515"
     CDS             complement(1373..2188)
                     /gene="proC"
                     /locus_tag="VIL39_02515"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY5480331.1"
                     /translation="MSAKLTIGFLGAGKMAAALARGFVRAGLLTARQVIASDPSEAAR
                     AAFAKEVGAKTTASNPDVARFANVLVLAVKPDQVSTVLADIRDHFTAKHCLISIAAGM
                     PLAKLEAGLGAGARLIRVMPNTPALVGASATAFALGKSARPEDGELAQKLFSAIGLAF
                     QVKEALLDAVTGLSGSGPAYVCLFIEGLSDGGVAAGLPREVATRLAAQTVLGSAKMVL
                     ETGLHPGVLKDMVTSPGGTTIEGLHELEKGKVRGALISAVRAAAEKSRKLGQG"
     gene            complement(2361..>2626)
                     /locus_tag="VIL39_02520"
     CDS             complement(2361..>2626)
                     /locus_tag="VIL39_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480332.1"
                     /translation="AIYEVLIPSDTPWAEMEKRIQQRKLGRKAAPQAPAFEPEANQEP
                     AETYDRGLHPLLLPGSARGSRMRFQAADPALETAQDLPVLSPV"
BASE COUNT          540 a          820 c          826 g          440 t
ORIGIN      
        1 tggatgtgag ggaattgctg gtgaaacgtg atgcgtgaaa cgtgatgcgt gaaacgtgaa
       61 ggcagcgtgg ccgccggatc acgtttcacg tttcacgttt cacgtttcac gcatcacgtc
      121 cagcccccgc ccgcttccac acgtagcgga acatcagcac ccggagcgca gccgtcagtg
      181 ggatcgccaa gatgccgccc agcaagcccc cgagcaaagt cgtgccagcc atgaccgcga
      241 tgatgatggt gacgggatgc agacccacac gcccgcccat gatcttgggg gagatgaacg
      301 caccttccag cgcctgaacg aatccaaata ccgcgagcac cagcagggga tgctgccagt
      361 cgccgaactg gacgatcgca atcacgagcg cggtgcagca ggtaacaatg gcgcccaaat
      421 agggaatcat cgtcaggacg acggccatgg cgccaatcag cacggcatag ggcaggccga
      481 tgataaggaa gccggtcccg taaagaatac cgtcgcaaat cgccaccaga acctgaccgc
      541 ggaagaagct gatgagatag ttgttgacgg agttgaggat gaacaccagt tcattcttga
      601 agccagagtc ggcaacgggc aggtaatccg tccagcgcga ggaaatgccg cgcttctcca
      661 gcagaaaata gaaggcgtaa acggggacca acgccagccc ggccacgaag ccgagccagg
      721 aagcgacccg tccgaactgg ccgaagagcc aatacccaac tttgggcagg gccccggcca
      781 gccacttggt ggccgtctgc aaactttcgt tgtcgagcgt gccgccgagg aataagggcg
      841 cattagtgct ctcaatggcg gcagcagtgg ggctgttggt gggaaaggcg ggcgcgctct
      901 catttgtcgc agtcagagaa tcgcgactct ctttcgcgcc cgattcccgc tccagcaacc
      961 gcttcaccag ggccggggga tggctcaccc aatgttccat gcgctgttcg acatgggccg
     1021 cgtaggccgg gatgcggacg gcgagctggc gggtttcatt gatcaactga ggcacgacgc
     1081 tgccgaaaag ggcggccaca atcaccaggg cgagggcgaa tacgcaaagg atggcgcggg
     1141 gccgcgacgc gcctttgcgc tcgatgaaat ctacgaccgg gtcaagcagg taggcaatca
     1201 cgcccgcgac ggcgattggc catagcactg gcgagagcat gcgcaacacc tggcccagcc
     1261 cccagacgag cgccacgacc agacccacca gcacggcgac cgccagaccc gtcaaggcca
     1321 gccagattac gcgcgcctgc cgcttggtgg gaggagggag gttcacgcca atctaaccct
     1381 gccccagttt cctcgacttc tcggccgccg cgcgcacagc gctaatgagc gcgccgcgca
     1441 ccttcccctt ttccagctcg tggaggccct cgatcgtcgt gccgcccggg ctggtcacca
     1501 tgtccttgag cacgccggga tgcaggccgg tttccaggac catcttcgcg ctgcccagga
     1561 ccgtttgggc tgccagccgg gtggcaacct cacgcggcag gcccgcggcg acgccgccat
     1621 cgctgagacc ctcgataaac aggcagacat aggccggccc gctgccgctc aagccggtga
     1681 cggcgtcgag caaggcttct ttgacctgaa acgccagccc gatggcggag aaaagcttct
     1741 gggccagctc gccatcttcc gggcgggctg acttgcccag ggcaaaggcc gtggccgaag
     1801 cgcccaccaa tgcgggtgtg ttcggcatca cgcgaatgag ccgcgcgccc gccccgagtc
     1861 ccgcttcgag tttggccagc ggcatgccgg cagcaatgga gatgaggcaa tgcttggcgg
     1921 tgaagtggtc gcgaatgtcc gccagcaccg tgctgacctg gtccggtttg acggccagca
     1981 cgaggacatt ggcaaacctg gccacatcgg ggttggatgc cgttgtcttc gctccaacct
     2041 ctttggcgaa cgcggcgcgg gcggcttcgc tcgggtcact ggcaataacc tggcgggccg
     2101 tgaggagtcc ggcccgaaca aagccccggg ccagggcggc ggccatcttt ccagcgccaa
     2161 gaaaaccgat agtgagcttc gcggacatgc cgggattgta ccctgtggtc tcgcgaagcg
     2221 gaagcgaaaa cccggcagcc ctggtgtaaa cctgcggcga ttttgtccgg aatgaagtag
     2281 ttcctgttcc aagatttctg tacatggaaa ttgcgcaccc tttcggcggg tgtgcaaaaa
     2341 actcagcctt taacgcccgg ttaaactggg cttaagactg gtaaatcttg agcggtttcc
     2401 agcgccggat cggcggcctg gaatcgcatc cggctgcctc tggcggagcc cggcaaaagc
     2461 aggggatgca accctcggtc ataagtctcg gcaggctcct ggttggcctc tggctcaaac
     2521 gccggcgcct gcggcgccgc cttgcgacca agcttccgtt gttgaatccg cttttccatt
     2581 tcagcccatg gcgtgtcgct gggaatcagc acttcgtata tggcgc
//
LOCUS       DATCGS010000127         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_556057, whole genome shotgun sequence.
ACCESSION   DATCGS010000127 DATCGS010000000
VERSION     DATCGS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556057"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VIL39_02525"
     CDS             complement(<1..627)
                     /locus_tag="VIL39_02525"
                     /inference="COORDINATES: protein motif:HMM:NF040425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HEY5480333.1"
                     /translation="MLQNPAGGRRPLKRPRLGSQQRLLNRQELQRENQQLREANERLR
                     REVAESEQRIADQEKQIADLERQLAGRKKDSTNSSKPPSSDGPAAARRLKPARCRGRR
                     KPGGQKGHPGRHRELQPPERVDETVAVLPVSCRHCDQPLPQQLQQVETRGEVHRYQVT
                     ELPPLRAHITEYQCHKVACPHCGRATRAELPSAVRGSAFGPRLAGLIAY"
     gene            complement(590..1012)
                     /locus_tag="VIL39_02530"
     CDS             complement(590..1012)
                     /locus_tag="VIL39_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480334.1"
                     /translation="MDIQTLQKHVLTLATIKETDAPMVSCYLNLEKGLTAARRILNER
                     VRLLRKTLPTPQRDSFEHALLGIESRLAAGFQTESLGAAVFARDGEQKVIVQLPLYRS
                     NQALDIFKKTLDIWKVMKYAGVDECEGAPESGGWTKAA"
     gene            complement(1061..1207)
                     /locus_tag="VIL39_02535"
     CDS             complement(1061..1207)
                     /locus_tag="VIL39_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480335.1"
                     /translation="MFSNIDDQIQQTNGEPVSRVKLLLRALGVFGVTTILFGRLYLGI
                     LLLE"
     gene            complement(1234..1599)
                     /locus_tag="VIL39_02540"
     CDS             complement(1234..1599)
                     /locus_tag="VIL39_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY5480336.1"
                     /translation="MFRNLDNQTQQNDRHHVSVMQRVLGVVGEEEHFARLEVEHGRKL
                     ADERRTKLLTEERERDCALHFMKCPKCGMQLEEIAFGDVRVAKCFSCDGLWLDKGEPD
                     LIRAKEGGFMGRLLSVFGG"
     gene            complement(1610..2035)
                     /locus_tag="VIL39_02545"
     CDS             complement(1610..2035)
                     /locus_tag="VIL39_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5480337.1"
                     /translation="MTGEQETATLIMQQPLNDAVTLLRRALAKGGLEVAADLDMAGRI
                     RKALRIDFPPCRVLCVDCPVALLEALALDRSAAVLLPLHLVVTGQDGLTLVHLLNPAA
                     ALYSGLPVTARAAVSKLQARVAQAVESVSIRQNPLEINA"
     gene            2334..>3351
                     /locus_tag="VIL39_02550"
     CDS             2334..>3351
                     /locus_tag="VIL39_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY5480338.1"
                     /translation="MSKTSVLENTPKPGRTSLDLTGFAGLSEDDARRRTAADGPNEIA
                     SQEKRSLFAIVIEVVREPMFLMLVAAGALYLVMGSPGDALMLLGFVFVVMAITIVQER
                     RTERALDALRDLSSPRALVIRGGIRRRVAGREVARGDILVLSEGDRVPADAILRRAIN
                     LSVDESLLTGESVPVRKTPSAEAKSLERPGGDDLPSVYSGTLVTAGQGIAEVVNTGGR
                     TELGKIGKALQQIQHEPTSLQMETERLVRTLALAGLAACGLVVVIYALTRGGAAQVWK
                     EGLLAGIAMAMAILPEEFPVVLTIFLALGAWRISRSRVLTRRMPAIETLGAATVLCVD
                     KTGTL"
BASE COUNT          618 a         1026 c         1028 g          679 t
ORIGIN      
        1 gtaggcgatc aggccagcca gacggggtcc aaaagcagaa ccccgcaccg cggagggaag
       61 ttcggcccgg gtggcccggc cgcagtgcgg gcaggccact ttatggcact gatactcggt
      121 gatgtgcgcc cgcaagggcg gcaattcggt gacttgataa cgatggactt caccccgcgt
      181 ctcgacctgt tggagttgct ggggcagagg ctggtcgcag tggcggcaac tcacgggcag
      241 cacggccacg gtttcatcga cccgttcggg cggctgcaac tcccggtggc gccccggatg
      301 gcccttttgt ccgcccggct tgcgccggcc ccggcagcgc gccggtttca atcgccgcgc
      361 ggcggctggc ccgtcggatg agggaggttt ggaggagttc gtggaatctt tcttccggcc
      421 ggcgagttgg cgctccagat cggcgatctg cttctcttgg tcagcgatcc gctgctccga
      481 ctcggcgacc tctcgccgaa gccgttcgtt cgcttcgcgg agttgctggt tctctcgctg
      541 cagctcttgc cggttgagta ggcgctgctg ggaacctagc cgtggccgct taagcggcct
      601 tcgtccaccc gccggattct ggagcaccct cgcactcatc taccccagcg tatttcatca
      661 ccttccaaat gtccagtgtt tttttgaaaa tgtccagcgc ttgattgctg cggtagaggg
      721 ggagctgaac gattaccttc tgttcgccat cccgcgcaaa gacagcggca cccagactct
      781 ccgtctggaa acccgccgcg aggcgggact caatgccgag gagggcgtgt tcgaaggagt
      841 cacgttgtgg cgtaggaagg gtcttccgca gcaaacgcac tcgctcattg agaatccgcc
      901 gcgcagcagt cagacccttc tcaagattca ggtaacaact gaccatggga gcgtcggttt
      961 ccttgatcgt cgccaaagtc aggacgtgct tttgcagcgt ttgaatgtcc atttgaaatg
     1021 ctctcctcta tcaagatcgt tatgtgtctt ccggccaaac ctactcgagt agcagaatcc
     1081 cgaggtagag gcgtccgaaa agaatggtcg tcacaccaaa cacgcccagt gcacgcagca
     1141 gtagcttcac gcggctgact ggttcgccgt tggtttgctg aatctggtca tcgatattgc
     1201 tgaacatggt gcctccttca gacacttgag tcgtcagccg ccaaacacgc tcaacagcct
     1261 ccccatgaag ccgccctctt tcgctcggat aagatcgggt tctcccttat ccaaccagag
     1321 gccatcgcaa gagaaacact tggcgacgcg cacatcgccg aacgcaatct cttcaagctg
     1381 cattccgcac ttcgggcact tcatgaagtg gagcgcacaa tctcgctccc gttcttccgt
     1441 caagagcttt gtccgccgtt cgtcggccaa tttccgcccg tgttcgactt ccaggcgggc
     1501 aaaatgctcc tcttcaccca caacaccaag cactcgctgc atcacgctga catgatgacg
     1561 gtcgttttgc tgagtttggt tatcgagatt gcgaaacatg atgcctcctt taagcattaa
     1621 tttccagtgg attctgccgg atcgatacgc tctcgaccgc ttgcgcgact ctggcctgta
     1681 gtttgctcac tgcagcccgc gccgtcacgg gcagtccgct ataaagcgcg gcggcaggat
     1741 tcaacaaatg aaccagagtt aagccgtcct ggccggtaac aaccaggtgc agcgggagca
     1801 ggacggcggc cgagcgatca agggcaagtg cctccagtaa tgccacgggg caatcaacgc
     1861 aaaggaccct gcacggtggg aaatcaatcc ggagggcctt cctaatacga cctgccatgt
     1921 cgagatctgc ggcaacctcc aggcccccct tggcaagcgc gcggcggagc aatgttacag
     1981 cgtcgtttag cggttgctgc atgatgaggg ttgcggtctc ttgctcgccg gtcattgggt
     2041 cgtctcttgc cttttcagtc agttagagtg cagttcactg gccgttcccg aatcgcggtc
     2101 aacctttctg aagaaccaag ggattggcgc tgagcgctca gaatgacaag cggcgcgcct
     2161 gtaaggtgct gcctcaaaac tggcagcgcc accgcttatc gggcagcctg agccttcggc
     2221 gccgccccac cgcatctgcg ctcataggtt cagagagtgg cctttggcgt gccccaaggt
     2281 tttgatgcgg cggagggttc gccgccatct atctggaggt tgcgcaaaca gggatgtcca
     2341 aaacaagcgt gttggagaat actccaaagc ccggccgcac gagcctcgac ctcaccgggt
     2401 ttgcgggcct cagcgaggat gatgcccgcc gaaggaccgc agcagacgga cccaacgaga
     2461 ttgcctcgca ggagaagcga tctctcttcg cgatcgtgat cgaagtcgtc cgcgagccga
     2521 tgttcctgat gctggtggca gcgggcgcac tctatctggt gatgggctca ccaggcgacg
     2581 cactgatgct cctcgggttc gtcttcgtcg tcatggcgat cacgatcgtc caggagcgcc
     2641 gcacagagcg cgctttggac gccctgcgcg acctgtccag cccccgcgct ttagtcattc
     2701 ggggtggcat tcgccgccgc gtggccggcc gcgaggttgc ccgtggtgac atcctcgtcc
     2761 tctccgaagg agatcgcgtg ccggccgatg ccatcctgag gcgcgcgatc aatctgagcg
     2821 tggacgagtc actgcttacc ggagaatcgg tcccggtgcg gaagacccct tcggcggaag
     2881 cgaagagcct cgaacggcca ggcggcgacg atctgccatc cgtatactcc ggaacgctgg
     2941 tgacggcggg ccagggcatc gcagaggtgg tgaacacggg ggggcgtacc gaactcggca
     3001 agattgggaa ggccttgcag caaatccagc acgagccgac gtcgttacag atggagacgg
     3061 agcggctggt tcgcacgctt gctttggctg ggctggcggc ctgcggcctg gtggtcgtca
     3121 tctatgccct cacgcgaggc ggcgcggcgc aggtctggaa agagggactg ctggccggaa
     3181 tcgccatggc catggccatc ctgccggagg agttcccggt ggtgctcacc atcttcctgg
     3241 ccctgggggc atggcggatc tcgcgcagcc gtgtgcttac gcgccgcatg cccgccatcg
     3301 agacgctggg ggcggcgacg gttctatgcg tggacaagac cggaacgctc a
//
LOCUS       DATCGS010000128         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_407440, whole genome shotgun sequence.
ACCESSION   DATCGS010000128 DATCGS010000000
VERSION     DATCGS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407440"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIL39_02555"
     CDS             <1..513
                     /locus_tag="VIL39_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480339.1"
                     /translation="DYLQAAAREKGKFRTFLLVALKRFLANEWDREHALKRGGFAQVV
                     SIDQEVAESRFAAEPSHNVQPDVLFDRQWAMTLLERTMTQLQEEYVASGRAKLFEYLQ
                     SCLAREESALPYAQIAACLNLTEPAVKMAVHRLRARYREILRAEIAHTVSSPEEVEEE
                     IRHLFASFGP"
     gene            597..800
                     /locus_tag="VIL39_02560"
     CDS             597..800
                     /locus_tag="VIL39_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480340.1"
                     /translation="MAKVLEAALGGQPTLIFRKGRLAIVKEYDPQTDSLSRLEVAFAE
                     GGGLNREPTVVERTLVSALSRRK"
     gene            819..1001
                     /locus_tag="VIL39_02565"
     CDS             819..1001
                     /locus_tag="VIL39_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480341.1"
                     /translation="MSFWPLPSGKAWVGNVGRFALAAITPLYLITRDSDLLSLGKPFG
                     IIVITDEQFLKLAKTF"
     gene            1284..>3233
                     /locus_tag="VIL39_02570"
     CDS             1284..>3233
                     /locus_tag="VIL39_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5480342.1"
                     /translation="MKSRALLQPGEQFGHYTIIRMLGADGMGAVYETEDLESGRRVAL
                     KVLSHRLDSPDARERFFREGRLAASINHPNSVYIFGTEEIGGTPVIAMELVSGGTLQE
                     RVRARGPLPIGEAVDCVLQIIEGLEAAQRIGILHRDVKPSNCYMGEDGVVKIGDFGLS
                     ISTAVRTEPALTATGAFLGTPAFCSPEQLRGEELNARSDMYSVGATLFYLLTGRTPFD
                     APNMVQLLATVLEQRAPSPRKLRPEVPPGLAKAVLRCLEKQPGERFKNYADLAQTLAP
                     YSSTAPTPATMSLRLLAGVVDMTILCVLATVINLSAFGSPMAFMNQAMQLSPKMLVCV
                     LGWIGVATLYFGLCESLWGAAAGKALCRLRVVGLDRNPPGFARACLRALAFFLPPLLP
                     YWTIFGMNPKAYMSSSQLTQMLLGLSIYGMMALLFVTARRRNGFAAVQDLLTGTRVIS
                     RAALSLRPVLPTISVPPPSVESAVTIGPYHVLQALTESAAESWYLAYDLKLLRKVSIR
                     TVPPGTEPVPVRLRSLSRIGRLRWLTGKRSPEENWDAFEALEGRPFLQLSGSPQPWRE
                     VRYWLYDLAAEISASEKDGTLPELALDRVWITGEGRAKLLDFPAPGPVGKSETRNPKP
                     ESSPGSPPQTTQGFLAEVAAAALEGT"
BASE COUNT          557 a          998 c         1022 g          656 t
ORIGIN      
        1 gactatttgc aagcggcggc gcgggagaag ggcaagttcc gcacttttct actggtggcc
       61 ctcaagcgct tcctcgccaa cgaatgggat cgggagcatg cgctgaaaag aggcggcttt
      121 gcgcaggtgg tgtccattga tcaggaggtc gccgagtctc gcttcgccgc ggagccgtcc
      181 cacaacgtcc agcccgacgt gctctttgac cggcaatggg ccatgacctt gctggagcgg
      241 accatgacgc agttgcagga ggagtatgtc gcctccgggc gggcgaagct gttcgagtac
      301 cttcaaagct gcctcgcccg ggaagagtcg gccctgcctt atgcgcagat cgccgcctgc
      361 ctgaacctta ccgagcccgc cgtgaagatg gcggttcacc ggttgcgcgc ccgctaccgc
      421 gaaatcctgc gcgcggaaat cgctcacacg gtctcctccc cggaggaggt agaggaagaa
      481 atccgccacc tctttgcgag ctttggcccg tgagccggct aaggtcttga actggtccgg
      541 atctggacta tccttggggc acgaaagtct atacgcccag ttacgtcaaa gtgaacatgg
      601 ccaaggtgct ggaggcggca ctgggaggcc agcctactct catcttccgc aaaggccgcc
      661 tggcgatcgt caaggaatac gatccgcaaa cggattcgct ttcgcggctc gaggtggcgt
      721 ttgctgaggg tggcggactg aatcgcgaac ccaccgtggt ggaacggacg ctcgttagcg
      781 ctcttagccg gcgcaaatga cggtcgtgat tgacacgaat gtctttctgg ccgctgcctt
      841 ctggaaaggc gtgggtcgga aatgttgggc gctttgcgct ggccgccatt actccattat
      901 acctcattac gcgcgattcc gacctattgt cgctgggcaa gccgttcggc atcatcgtta
      961 ttactgacga gcagttccta aagctggcca agacattcta ggttgggcga attgtcgagc
     1021 tttctgtgtc tcccgggagc cggcggtccc ggaataatta tgggactgcc gcgttacctt
     1081 ttccgggttc tgcttaagca cccttggaac gttatggcta atcttgagaa tacatcttct
     1141 gcgaagcgca aatgcgtggg gtgtggcgcc gaattgcccg gcaacacgcc gcctgacctt
     1201 tgcccgaaat gcctcctaaa actcgccatg gagacccagc cgggtcctgg acccggggca
     1261 ccgttgtgct ttccggggcg gaagtgaaat cccgtgccct gttgcagccg ggcgagcaat
     1321 ttggccacta caccatcatc cgcatgctgg gcgcggacgg catgggcgcg gtctatgaaa
     1381 ccgaagacct ggagagcgga cgccgggtgg cgctcaaggt cttgagtcac aggctcgatt
     1441 cgccggacgc gcgcgagcgt ttcttccgcg aggggcgcct ggccgcttcg atcaaccatc
     1501 ccaacagcgt ctatatcttc ggcaccgaag agatcggcgg cacgcctgtg atcgcgatgg
     1561 agctggtgtc gggcggcacc ttgcaggaac gcgtgcgcgc ccgggggccg ttgccaattg
     1621 gcgaggcggt ggactgcgtc ttgcagatca tcgaaggttt ggaggcggcc cagcgcatcg
     1681 gcattctcca ccgggacgtt aagccctcga actgctatat gggcgaagac ggcgtggtga
     1741 agatcggcga cttcggcctc tcgatttcga ctgccgtgcg caccgagccc gcgctgaccg
     1801 ctaccggcgc ctttctcggc acgcccgctt tctgctcgcc ggaacaattg cgcggcgagg
     1861 aactcaacgc tcgctcggac atgtattcgg tcggcgccac cttgttttac ctgctcaccg
     1921 ggcgcacgcc ctttgatgcg ccgaacatgg tgcaactcct tgccaccgtg ctggagcagc
     1981 gcgcgccgtc accccggaag ttgcgtccgg aggtgccgcc agggctggcc aaagcagtgc
     2041 tgcgttgtct cgagaagcag ccgggtgaac gcttcaagaa ctatgccgac ctggcccaga
     2101 cgctggcgcc ctacagttcg accgcgccga cgccggccac tatgagcctg cgcttactgg
     2161 cgggagtggt ggatatgacg atcctatgtg tgctggcgac ggtaattaac ctgtcggcat
     2221 ttggatcacc catggccttc atgaaccagg ccatgcaact ctcgccgaag atgctcgtct
     2281 gcgtgctggg atggatcggc gtggcgacac tttactttgg gttgtgtgag agtctgtggg
     2341 gcgccgcggc gggcaaggcc ctctgccggt tgcgcgtggt tgggctggac cggaatccgc
     2401 ccggcttcgc gcgggcctgt ctccgcgcgc tggcgttctt cctgccgccg ctcctgcctt
     2461 actggaccat ctttggcatg aatccgaagg cttacatgag ttcctcgcag ttaacgcaga
     2521 tgctcctggg cttgtccatc tatgggatga tggcgctgct gttcgtcacc gcccgccggc
     2581 gcaacggctt tgccgccgtg caggacctgc tgaccgggac cagggtgatt tcccgggctg
     2641 ccctcagctt gcggcccgtg ctgcccacta tcagtgtgcc gccgccgtcc gtagagtcgg
     2701 cggttaccat cggcccgtat catgtgctgc aagcgcttac ggagtctgcc gccgagagct
     2761 ggtatctggc gtacgacctg aagcttttgc gcaaggtgtc tattcgcacg gtgccccccg
     2821 gcacggagcc ggttccggtc cggctgcgca gcctgagccg gattggccgc ttgcgttggc
     2881 tcaccgggaa acggtcgccc gaagagaatt gggatgcctt tgaagccctg gagggccggc
     2941 ctttcctgca attgagcggt agccctcaac cgtggcgcga ggtgcgttat tggctctacg
     3001 acctggccgc cgaaatcagc gcctcggaga aggatggcac cttgcctgaa ctcgccctcg
     3061 accgcgtctg gatcactggc gaaggccgcg ccaagctgct cgacttcccc gctcccggcc
     3121 cggtggggaa atccgaaacc cgaaacccga aacccgaatc aagtccaggt tctccacccc
     3181 aaacaaccca aggcttcctg gccgaagtgg ccgcggcggc attggaaggg act
//
LOCUS       DATCGS010000129         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_160420, whole genome shotgun sequence.
ACCESSION   DATCGS010000129 DATCGS010000000
VERSION     DATCGS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160420"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..628)
                     /locus_tag="VIL39_02575"
     CDS             complement(215..628)
                     /locus_tag="VIL39_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY5480343.1"
                     /translation="MNAFPVGPDKEAQLAQRMAALGVREADIEETFVRSGGHGGQNVN
                     KTATCVMLLHRPTGLQVKCQATRQQGLNRFLARRLLLDKVEALKNGYVATERAATEKI
                     RRQKRRRSRRAKQRMLADKSHHAAKKDARRPVTGD"
     gene            761..1552
                     /locus_tag="VIL39_02580"
     CDS             761..1552
                     /locus_tag="VIL39_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY5480344.1"
                     /translation="MKLLFIADIVGQPGRRAVLRLVPQLRQRHGLSLVVANGENAAGG
                     SGITMNTAEEIFAAGVDIITSGDHLWDQKEVMALLEGERRFVRPLNYPPGTPGQGSTI
                     FQGQGLPPVAIMNLQGRTFMAAIENPFHAALAEVERLRRTTKIIFIDFHAEATSEKVA
                     MARMLDGRVSALVGTHTHVQTADEQIFPGGTAFLCDGGFTGPHESVIGREIEPVVRRF
                     VTNMPQKFEVAKGRVLLQGAVVEIDEQTGRATGIQRVSEAVEPNF"
     gene            1552..2964
                     /gene="xseA"
                     /locus_tag="VIL39_02585"
     CDS             1552..2964
                     /gene="xseA"
                     /locus_tag="VIL39_02585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5480345.1"
                     /translation="MAKPVKSQWDFGELFPPDAIRKVLSVTELTWQIKRLLEKQVGEI
                     WVTGEVTNLRSQSSGHIYFTIKDAGSQLNCVLFRGEAQVDRSLLQDGRKVTLQGDVTV
                     YEPRGQYQLRVTKVELQGIGALLAAFEKLKQALKAEGLFATERKRALPRYPQRLGLVT
                     SPTGAAIRDVLHVVERRNPALEIILVPCRVQGDGAAAEIAAAIRLLNEFSSAQLAIGN
                     RQSAIDLILVTRGGGSLEDLWAFNEEVVARAIFDSALPVVSAVGHEIDFTISDFVADV
                     RAATPSAGAEIITEGVFSSCQFLSEAARRIGELARQQIEDKRYGLAQTTQRLARVHPR
                     RRLNDWLQRLDDAQTSLLRCVKQGARRQRLTWQNLSERLGRVRPALVLKQRREAFGQI
                     ERRLHEQVRQQLETRRIGLGALEARLRLLGPEQVLARGYSITTDAATGKVLRKAKEVK
                     AGQRLKTRLKAGEVFSAVEQ"
     gene            complement(3006..3713)
                     /gene="pdxH"
                     /locus_tag="VIL39_02590"
     CDS             complement(3006..3713)
                     /gene="pdxH"
                     /locus_tag="VIL39_02590"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5480346.1"
                     /translation="MSIADLRREYNLTGLRRRDLDADPIGQFKKWFDQAAGARASGRL
                     RKFGINLYKKLLMASGAELMDLTAMTLATADKEGRPSARVVLLKGVDQRGFVFYTNYD
                     SRKGHELAENPQAALVFYWSEQEREVCVAGEVSKLPPAESDAYFKSRPRGSRLGACVS
                     LQSTVVADRAALEEKWKQMDAQYAGQEIPRPPHWGGYLLCPARIEFWQGRPNRLHDRF
                     RYTRQPDNTWLIERLSS"
     gene            complement(3822..>4024)
                     /locus_tag="VIL39_02595"
     CDS             complement(3822..>4024)
                     /locus_tag="VIL39_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480347.1"
                     /translation="RVIIEWRSLLRQIAHAPDLDWPRWGDLQAMAKTILNETESPTLT
                     QLPPLEFHQTRRVDHRLILRRH"
BASE COUNT          717 a         1159 c         1404 g          744 t
ORIGIN      
        1 ccaaggctac cccaaggcta ccccaaggct accccaaggc taccccaagg ctaccccaag
       61 gctaccccaa ggctacccca aggcctagtg atagccaacc gattgcaact ccaaagacat
      121 ctcgaagtcc ggggaaagca aggcgaaagc gggggaagcg gcctgatttt gggaccttgc
      181 cccttggcgc ttggggtctg cgggtgttca actgctaatc gcccgtgact gggcggcggg
      241 cgtccttctt ggcggcgtgg tgggatttgt cggcgagcat gcgctgtttg gcgcggcggc
      301 tgcgacggcg tttctggcgg cgaatcttct ccgtggcggc gcgctcggtg gcgacgtagc
      361 cgttcttgag ggcttcgacc ttgtcgagca acaggcggcg agccaggaag cggttgagtc
      421 cctgctggcg ggtggcctgg catttgacct gcaagccggt cggacggtgg agcaacatga
      481 cgcaggtggc ggtcttgttg acgttctggc cgccgtgccc gccggaacgg acaaaggttt
      541 cttcgatgtc ggcttcgcgc acgcccaatg cggccatgcg ttgggcgagt tgggcctctt
      601 tgtcggggcc gacggggaat gcgttcatgg cggcaggata gcgggcggag ggagggattg
      661 caatgggcgg ggggagggtg gtgcgtgatt cgtgattcgt gatgcgtgat gcgtgggcga
      721 aacggcttgc cgagcggcgt tggggcatta ctttgggggc gtgaaattgc tgttcattgc
      781 ggacatcgta gggcaaccgg gccggcgcgc ggtcttgaga ctcgtccccc aattgcggca
      841 gcgacatggc ctgagcctgg tggtggccaa cggcgagaac gccgccggcg gttctggcat
      901 cacgatgaat acggcggagg aaatcttcgc ggcgggtgtg gatatcatca cgagcggcga
      961 tcatctctgg gaccagaagg aagttatggc gttgctcgaa ggcgagcgcc ggtttgttcg
     1021 cccgctgaac tatccgcccg ggacgccagg gcaggggagc acaatcttcc aggggcaagg
     1081 gctgccgccg gtggcgatta tgaacctgca gggccggacg ttcatggcgg caattgagaa
     1141 tcccttccac gccgcgctgg cggaggtgga gcgcctgcgc cggacgacta agatcatctt
     1201 cattgatttc catgccgagg cgacgtcgga gaaggtggcg atggcgcgga tgctggatgg
     1261 ccgcgtgagc gccctcgtag gcacgcatac gcacgtgcag acggcggacg agcagatctt
     1321 ccccggcggg acggcgtttc tgtgcgatgg cggcttcacc ggtccgcatg agagcgtcat
     1381 cgggcgggag atcgagccgg tcgtccggcg tttcgtgacg aacatgccgc agaagtttga
     1441 ggtggcgaag gggcgtgtgc tattgcaggg ggccgtggtg gagattgatg agcagacggg
     1501 gcgggcgacg ggtattcagc gcgtgtcgga ggccgtggag ccgaacttct aatggccaag
     1561 ccagttaagt cgcagtggga tttcggcgag ttgttcccgc cggacgcgat ccgcaaagtg
     1621 ctgtccgtca ccgaactgac gtggcagatc aagcggctgt tggaaaagca ggtgggcgag
     1681 atttgggtga cgggcgaggt gacgaacctg cgcagccaaa gctccgggca catctatttc
     1741 accatcaagg atgccgggtc gcagttgaac tgtgttctgt ttcgaggcga ggcgcaggtg
     1801 gatcgctcgc tgttgcagga cgggcggaag gtcacgctgc aaggcgacgt gacggtgtat
     1861 gagccgcgag ggcaatacca gttgcgcgtg acgaaggtgg agctgcaagg catcggggcg
     1921 ctcctggcgg cgttcgagaa gctcaagcag gcgctcaagg ccgaagggct ctttgcgacg
     1981 gaacggaaac gcgcgctacc gcgttacccg cagcgcctcg gtctggtcac ctcgccgacc
     2041 ggcgcggcca tccgcgacgt gctgcacgtg gtcgaacgcc gcaaccctgc gctggagatc
     2101 atcctggtcc cgtgccgcgt gcaaggggac ggagcggcgg cggaaatcgc cgcagctatc
     2161 cggttactca atgagttcag ctctgcccaa ttggcaattg gcaatcggca atcggcgatt
     2221 gaccttatcc tggtcacccg cggcggcggc agcctggagg acttgtgggc gtttaacgaa
     2281 gaagtggtgg cgcgcgcgat ttttgactcg gccttgccgg tcgtctcggc ggttgggcac
     2341 gagattgact tcaccatcag cgattttgtg gccgacgtgc gggccgcgac gcccagcgct
     2401 ggcgcggaga tcatcacgga aggtgtcttc tcaagctgcc aattcctgtc tgaggctgcc
     2461 cggcgcatcg gcgaattggc caggcagcaa atcgaggaca agcgttacgg cctggcgcaa
     2521 accacgcagc gccttgcccg ggtccacccg cggcgccggc tcaatgactg gctgcagcgt
     2581 ctggatgacg cgcaaacgag cctgctgcgt tgcgtcaaac agggcgcgcg ccggcagcgg
     2641 cttacctggc agaatctctc ggagcggctg gggcgtgtgc gtccggcgct cgttctgaag
     2701 cagaggcgcg aggcttttgg ccagatcgag cgccgcctgc acgaacaggt gcgccagcaa
     2761 ttggagacga ggaggatcgg gttgggcgca ctcgaagccc gcttgcgcct gctgggtccc
     2821 gagcaggtcc tggcccgcgg ctactcgatc accacggacg cggcgaccgg gaaggtcttg
     2881 cgcaaggcga aggaagtgaa agccggccaa cgcctgaaga cccggttgaa agcgggcgag
     2941 gtgttcagcg cggtggagca atagtcacgg atagacggcg cagtggaagc gttgcgtgcg
     3001 cttgctcaag acgacaaacg ctcaatgagc caggtgttat ccggctgccg ggtgtagcgg
     3061 aagcgatcgt gaagccggtt gggccgccct tgccagaact caatgcgcgc cgggcacagc
     3121 aggtaaccgc cccaatgagg cggtcggggg atttcctggc ccgcgtattg agcatccatc
     3181 tgcttccatt tctcctcgag ggccgcccgg tcggcaacaa ccgtgctttg cagcgagaca
     3241 cacgccccaa gccggctccc gcgcggccgc gacttgaaat aggcgtcgga ttccgccggc
     3301 ggcaacttgc tgacctcccc ggctacgcag acctcccgct cctgctccga ccagtagaag
     3361 accagcgccg cttgcgggtt ctccgccagt tcgtgcccct tgcggctgtc gtaattggtg
     3421 taaaaaacga atccccgctg gtccacgcct ttgagcaaga ccacacgcgc cgaaggccgg
     3481 ccttctttgt cggcggttgc cagcgtcatt gccgtcaggt ccatcagctc ggcgcccgag
     3541 gccatcagca gcttcttgta caggttgatg ccgaacttcc gcagccgccc gctggcgcgc
     3601 gcgccggctg cctggtcgaa ccacttcttg aactggccga tcgggtccgc atccaggtcc
     3661 cttcgccgca aaccggtcag gttgtattct cttcgtaaat ccgcgataga cataaagcac
     3721 ctaatggaca cttcggatcg tgagtttccg ccaaagcccg ccacccatca agctggaaac
     3781 ccgctgctta agtgatgagt agtctcttgt ggggcattga ttcaatgccg ccggagaatc
     3841 agccggtgat ccacgcggcg ggtttggtgg aattcgagcg ggggcaactg ggtaagcgtc
     3901 ggggactcgg tttcgttaag aatggttttg gccatcgctt gcaagtcgcc ccagcggggc
     3961 cagtcgaggt ccggcgcgtg agcgatctgt cgcaagaggc tgcgccattc gatgatgacg
     4021 cgat
//
LOCUS       DATCGS010000130         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_407500, whole genome shotgun sequence.
ACCESSION   DATCGS010000130 DATCGS010000000
VERSION     DATCGS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407500"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VIL39_02600"
     CDS             complement(<1..323)
                     /locus_tag="VIL39_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEY5480348.1"
                     /translation="MSKFKCLLLALALYSFAALNVAKAETFGLDTLQLQYIEQGWGEP
                     HANQSVDGHPMMLEGKRFEHGIGTHANSIFRIALGGKAERSTATVGVDDEVGQKGSVV
                     FKVTG"
     gene            complement(633..1238)
                     /locus_tag="VIL39_02605"
     CDS             complement(633..1238)
                     /locus_tag="VIL39_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY5480349.1"
                     /translation="MKTRHRLQTPTPGLQPTEAIHQHLGSRVKHFRSARGWSLEALAN
                     ASGVSRSMLSQIEREQANPTLAVTLRIARAFGLSLGELLEMPGAASSVTVIRADDHTY
                     HYRSDKDCRIRTLSPLNLEKDVEFYEILLQAGGSLRSAPHFEGTREFLTVQKGRIRVE
                     SGGDAEELNPGDSSSYRADVPHAIVNSGKGAATVFLIVIYR"
     gene            1329..2357
                     /gene="tdh"
                     /locus_tag="VIL39_02610"
     CDS             1329..2357
                     /gene="tdh"
                     /locus_tag="VIL39_02610"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY5480350.1"
                     /translation="MKALVKRERKPGLWLEDVPEPEVGINDVLIHVDRTGICGTDLHI
                     YKWDEWSQRTIPVPMVVGHEFVGEVVKVGSNVKDFVTGETVSAEGHVVCGRCRNCLAG
                     RRHLCRDTLGIGVNRPGAFAEYISVPMTNVWHHDNGIDRDVAAIFDPFGNAVHTALSF
                     PVLGEDVLIAGAGPIGIMAAGVVKHAGARYVVVTDVNDYRLDLARQMGVTVALNVKNG
                     RTLKDVQQQLGMKEGFDVGLEMSGNPAALCQMIDIMCHGGKIAMLGIQSEQAPFDWSK
                     VVFNMLTIKGIYGREMYETWYKMTVMLETGLNIKPAITHRFHYSEFEKGFEVMMTGKS
                     GKVILNWR"
     gene            2354..>2680
                     /locus_tag="VIL39_02615"
     CDS             2354..>2680
                     /locus_tag="VIL39_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480351.1"
                     /translation="MKSGTFLLLLALSGGAAGASAELRVPACTAYLDPELEGAHVSQR
                     SGITGWTNPALKVLWFGEMKHPGKLDCSLDLRLPAGAKTRLRMTMAGQSHEASAESSG
                     DTVVTVR"
BASE COUNT          529 a          746 c          855 g          550 t
ORIGIN      
        1 tcgccggtga ccttgaacac gacgctgccc ttttggccga cttcatcatc aaccccgact
       61 gtggctgtgg accgctccgc cttgcctccg agcgcaatgc gaaagatgct gttggcatgg
      121 gtcccgatgc catgctcgaa gcgcttgcct tcgagcatca tcgggtggcc gtccaccgac
      181 tggttggcgt gcggctcacc ccagccctgt tcgatgtatt gaagctggag agtatcgagg
      241 ccaaaggtct cggcttttgc cacgttcagc gcggcgaatg aatacagagc cagggcgagt
      301 aagaggcatt tgaatttgct cataagttta ggactagttt tacaataata ggcagttgct
      361 tgagttctcc aaccgttttg gacaaatcca gattcccgtg cgcccggagg cgacgagttc
      421 tcgcgaccag cacgcgtcca gaagagaaac actgcctgca tatcacgtct ggccgcgagg
      481 aattgaacca tacccaaccg ggacggtcaa gccgccccct cgcggcttgt gatcgatagg
      541 cttagagaga aaagcctggc aaatcaaaag ctgattacct ttgaaaggtg aaacaaaccc
      601 accccgccag gaggggccag gcggttcgag gcctaacggt agatcacgat taggaaaacg
      661 gtggcggcgc cctttccgct attgacgatg gcgtgaggca cgtccgcacg gtagctggag
      721 gagtcacccg ggttgagttc ctcagcatcg ccccctgatt ccacccggat gcggcccttt
      781 tgcacggtga ggaactcccg cgtcccttca aaatgcggtg cgctccgcag ggagccgccg
      841 gcctggagga ggatttcgta gaactcgacg tccttctcca ggttcagcgg cgagagcgtg
      901 cggatccggc aatccttgtc cgagcgatag tggtaggtgt gatcgtcagc gcggatgacg
      961 gtgacgctgg aggcggctcc cggcatttcg agcagctccc ccaggctcag gccgaaggcg
     1021 cgggcgatgc gcagggtgac cgccagcgtc ggattcgcct gctcccgctc gatctggctc
     1081 agcatcgagc ggctcacccc gctggcattg gccagggctt ccagcgacca gccgcgggcg
     1141 ctccggaagt gtttgacccg gctgcccaga tgttggtgaa tggcctcggt cggttgcagg
     1201 cctggcgtgg gtgtttgcag gcggtggcgg gtcttcatcg tgttcaatat actggaaaca
     1261 gttcttgcat agtggatgct gctctgttta tgatacggga tattgatcca ttatactaga
     1321 actatctcat gaaagcgctg gttaagagag aacgaaagcc cggcctctgg ctggaggacg
     1381 tgcccgagcc tgaagtcggc atcaacgacg tgctcatcca cgtggatcgc acggggattt
     1441 gcggcacgga tttgcacatc tacaagtggg atgaatggtc gcagcggacc atcccggtgc
     1501 caatggtcgt cggccatgag tttgtcggcg aggtggtgaa agtcggctcg aacgtgaagg
     1561 actttgtcac cggtgaaacc gtcagcgctg aagggcacgt ggtgtgcggg cgatgccgca
     1621 attgcctggc gggccggcgg catctctgca gggacacgct cgggatcggc gtcaatcggc
     1681 ccggggcctt cgccgaatac atctccgtgc cgatgaccaa cgtttggcac cacgacaatg
     1741 gcattgaccg ggatgtggct gctattttcg acccgttcgg caacgcggtg catacggcgc
     1801 tctcctttcc ggtgttgggc gaggatgtgc tgatcgcggg cgccggcccc atcggtatca
     1861 tggcggcggg cgtggttaaa catgccggcg cgcgctacgt ggtggtgacc gatgtcaatg
     1921 attaccggct cgacctggcg cggcagatgg gcgtcacggt ggcgctcaat gtgaagaacg
     1981 ggcgcacact caaggacgtg cagcagcagt tgggcatgaa ggaagggttc gacgtggggc
     2041 tggaaatgtc cggcaacccc gccgcgctgt gccagatgat tgacattatg tgccacggcg
     2101 gaaagattgc catgctcggc attcagtccg agcaagcgcc ctttgactgg agcaaggtgg
     2161 tgttcaacat gctcacgatc aaaggcattt acggccggga aatgtacgag acgtggtata
     2221 agatgacggt catgctggaa actgggctta atatcaaacc ggccattacg catcggttcc
     2281 attactccga gtttgagaaa ggcttcgagg tgatgatgac cgggaaatca ggcaaagtga
     2341 tcttgaactg gcgatgaagt ccgggacttt cctgctcctt ctggcccttt ctggcggtgc
     2401 cgctggcgct agcgcggagt tgcgcgttcc tgcctgcacc gcctacctgg acccggaact
     2461 ggagggggca cacgtctcgc agcgctccgg aataactggc tggacgaacc ccgcgctcaa
     2521 ggtgctctgg ttcggcgaga tgaaacatcc cggcaaattg gattgctcgc tcgatctgcg
     2581 cttgcctgcc ggggccaaaa cccggctgcg tatgacaatg gcaggccaat ctcacgaagc
     2641 ctcagccgag tcgtctggcg acactgtggt tacggtgcgg
//
LOCUS       DATCGS010000131         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_160588, whole genome shotgun sequence.
ACCESSION   DATCGS010000131 DATCGS010000000
VERSION     DATCGS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160588"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..158)
                     /locus_tag="VIL39_02620"
                     /pseudo
     CDS             complement(1..158)
                     /locus_tag="VIL39_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
     gene            complement(229..702)
                     /locus_tag="VIL39_02625"
     CDS             complement(229..702)
                     /locus_tag="VIL39_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5480352.1"
                     /translation="MEIKIRQAALADAQVIAAFNLRLAEESEGLRLAEAVVHAGVAAL
                     LKDPAKGIYYVAEVEGSVVGQVMITYEWSDWRNGNMWWIQSVYVKPEFRRAGVFRALF
                     NHLRTLAQTQKDVCNLRLYMHAENTRARQSYERLGMTRTHYEIFELDVRKDGERR"
     gene            complement(787..1275)
                     /locus_tag="VIL39_02630"
     CDS             complement(787..1275)
                     /locus_tag="VIL39_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY5480353.1"
                     /translation="MTSLAASPSLAPSVILNWDASPDTNIAGYKLYYGTNSGSYPQVV
                     SAGLVTQTTVTGLTPGTPYYFAATASDSTGLESDYSNEITFTMPALRPGQSLPELRLF
                     QAGTKSILQWPANYPGYTLQCSSSPAGEWTDLTSSPSISGSNFIYTNTVSAAQQYYRL
                     KE"
     gene            1648..2082
                     /locus_tag="VIL39_02635"
                     /pseudo
     CDS             1648..2082
                     /locus_tag="VIL39_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002742815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
     gene            2152..2421
                     /locus_tag="VIL39_02640"
     CDS             2152..2421
                     /locus_tag="VIL39_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480354.1"
                     /translation="MKAVTVREAQGQLGELIAEACRGEFIVLTDGDRQVALEPRVALD
                     LEEDSPELEAELLRAVNGPHAPLAEGELREAADKALAEHRARRQK"
     gene            2418..2759
                     /locus_tag="VIL39_02645"
     CDS             2418..2759
                     /locus_tag="VIL39_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY5480355.1"
                     /translation="MTLTLHRSDDFNRDFDLQYRWYLQQAGEAVAERYLQALLGTLRL
                     LATQPGVGRQRKFRHPALRGIRSFRLASPFEVHLIFYRAHAGELVAERLMHGARDLPR
                     RLVEPPGSTEG"
     gene            2749..2970
                     /locus_tag="VIL39_02650"
                     /pseudo
     CDS             2749..2970
                     /locus_tag="VIL39_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002742815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
BASE COUNT          577 a         1061 c         1052 g          656 t
ORIGIN      
        1 ggcttgacct cacggctgac gatggtctgc agggcgagga tgatctgcgc cgccaggacc
       61 accgggtcct tggtcttgtg cggtgctgcg ccgtgcccgc ccacgcgctt aacgatgatg
      121 tccacggaat cgatgttggt cccggaatag ccgggcgtgc caggtcatgt ggaagtcgtg
      181 gccgcaggca tgcatgacgg gcacgtccgt attgcctggc agactccgtc atcgccgttc
      241 accatccttt cgcacgtcca gttcgaagat ctcgtaatgt gtgcgggtca tcccgaggcg
      301 ctcgtaggat tggcgggcgc gggtgttctc cgcgtgcatg tagagccgga ggttgcaaac
      361 atccttctgc gtctgggcca gagttcgcaa gtggttgaaa agcgcgcgaa acacgccggc
      421 gcgccggaac tccggcttca cgtacacgct ctggatccac cacatgttgc cgttgcgcca
      481 atcgctccat tcgtaggtga tcatcacctg ccccaccacc gagccctcca cttcggcgac
      541 gtagtagatg cctttggcgg ggtccttcaa caaggcggcc acgccggcgt gaaccacggc
      601 ctcagccagg cgcagacctt cactctcttc agccagccgc aggttgaagg cggcaatcac
      661 ctgcgcatcg gctaaagccg cttgccgaat cttgatttcc atgcccgatc ttaagaggcg
      721 tgccgcgaga atcaaatcgt ttcccgttga gtccgccacc gccggagaca gcccttctcc
      781 accccgtcat tccttcaacc gataatactg ctgcgccgcg ctcaccgtgt tcgtgtagat
      841 aaaattgctc cccgagattg acggactgga ggtcagatcg gtccactccc ccgccgggct
      901 gctgctgcat tgcagtgtgt acccggggta gttggcaggc cattgcagga tcgattttgt
      961 cccggcctgg aagaggcgta gttccggcag gctctgtccc ggccggagcg caggcatggt
     1021 gaacgtaatt tcgttcgagt agtcgctctc caggcccgtg ctgtcgctcg ccgtcgcggc
     1081 aaaataatag ggggtccctg gcgtcagccc ggtaaccgtc gtttgtgtca ccagcccagc
     1141 cgacaccacc tgcgggtagc tgccgctgtt cgtgccgtaa tagagcttgt aacctgcgat
     1201 gttggtgtcc gggctggcat cccagttcag gatcacgctt ggggctagcg acggactggc
     1261 agccaggctg gtcaccgccg gggttatata aagcaagctg caaaccactg caagactgag
     1321 gccgccagcc aggggccggc gcagtctgtc gaaactcgga tgtcgagtcg ctttatgcac
     1381 gccccctata gcaacggaac cgccatactc tgcgccgaac cgcgcctttg cactgaacgc
     1441 gcagttttcg tcagcgaact caacaagtca tggtgctccg gcctcttccg ccccccaccc
     1501 ggagacctgt gaacgtcaaa aaatagtacg ttgtgtcccg gttgcccacg ccgagtccgc
     1561 gcgccactgg gacgcggaac tccagcccgc ttagccctgc tgcggtgccc ctcccattta
     1621 taccccgctt gagcggttta ccaccgagcc agccgcttct tgttcggtgt actcacctca
     1681 gccatgcaca tggcgtcggt gcggcaaatc ggcggtcgct tggaatctcg ctaccgctat
     1741 tccggcagca tggtttacaa caactacccc tggccggaga aggcgacggc caagcagcgg
     1801 acggcggtgg aaacggcggc gcaggccgtg ttggacgcgc gggcgcagta cccgacctcg
     1861 acgctagcgg atttgtatga cccgttgacg atgtccgcgc cgctgctcaa ggcgcatcag
     1921 caactagacc gcgcggtgga ccgctgttac cggcccgagc cgttccccag cgaccggcac
     1981 cgcgtcgaat acctcttcgc ccgctacgag cagttcaccg cccccctggt cgccgccgcc
     2041 aaggccgccc gtaaagggcg gaaggtccaa gcaatcgctt aacgctaaca agtaatcagt
     2101 ggtgagaggt tgccgttgct ttcggccgtg cggatggcta cattaggtgc catgaaagcg
     2161 gttaccgttc gggaagccca gggacagctt ggagaactca tcgccgaggc gtgccgcggt
     2221 gagtttatcg ttctgaccga cggcgacagg caggtggctt tggagccgcg tgtcgcactg
     2281 gacctggagg aggacagccc ggagttggag gcggagctgt tgcgggcggt caacgggcct
     2341 catgccccgc tggcggaagg cgagctgcgg gaagccgcgg acaaggccct ggcagagcac
     2401 cgggcccggc gccagaaatg actctgacgc tgcatcgctc cgatgatttc aatcgcgact
     2461 tcgacctgca ataccggtgg tatcttcagc aggcgggcga ggcggtagcg gagcgttacc
     2521 tgcaagcttt gctgggtacg ctgcgtctgc tggctacgca acccggggtg ggccgccagc
     2581 ggaagtttcg gcatccagct ctccggggca tacgctcatt ccggctggca tctccgtttg
     2641 aagtgcacct gatcttctac cgcgcccacg ccggcgagct tgtggctgag cggctgatgc
     2701 atggtgcgcg cgatttgccg cgacggttgg ttgagccgcc gggctcgacg gagggctgag
     2761 gccgtggaag catcggccca ggcggtgttg gacgcgcggg cgcagtaccc gatctcgacg
     2821 ctgtctgatt tgtatgaccc gttgacgatg cccgcgccgc tgctcaaggc gcatcagcaa
     2881 ttggaccgcg cggtggaccg ctgttaccgg cccgagccgt tccccagcga ccggcaccgc
     2941 gtcgaatacc tcttcgccct ctacgagtag ttcaccgccc cccctggtcg ccgccgccaa
     3001 gcccgcccgt aaagggcgga gaaggccaac gacgccatga ggtcgttcgg cccggttgag
     3061 ttcggagggc cggtcagagg agtttggagt tcgggcttgt cggattgccc ggttgcaact
     3121 gactgcactg ccatgatctg ccttcgtccg gggagtttgg atgcgaggcg tcgcctgagg
     3181 ccctgtccag aagccggaag ccagcctctg gtccgaacgg gaacagaggg acctgccagt
     3241 gtggtgcaag ccaggagccc gcgggatgct ctctattatt cctctattgc tcctctattg
     3301 ttcctttggt cctggtcctt cggcacagcg gggctccgat gggagt
//
LOCUS       DATCGS010000132         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_357844, whole genome shotgun sequence.
ACCESSION   DATCGS010000132 DATCGS010000000
VERSION     DATCGS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357844"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VIL39_02655"
     CDS             complement(<1..1415)
                     /locus_tag="VIL39_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY5480356.1"
                     /translation="MDITSAVTAGQTADVRVLVAAIADAEQVGTFWQNAFMNVSYTAA
                     SLKSGGLTGSVFLESRSSEARVTDVFVRTSTRKKEISLDVELAGVKHAGPVHVVADML
                     NEKGEVEKSFAADAVVISAGTQAFAVSWPWADPRLWDVGQPDLYTLRLKVKGAGLDDE
                     YNQKFGFREFWIEGRQFYLNGTVIHLRQPCFYNGSRMQMGDIFSEMGEGNVDARGDAA
                     DSGRNLEDADRKGYLVAHYILNANKYLMSSKGRFVWEQNRQRAFDRAAVWMRHYHNHP
                     SVVIWIAGFNFFNNAVDADPRHLGRRGWDQSDERWQRLMLFGKEMFDGLKKLDQTRAY
                     YSHSGAYTGDIYTMNCYLDMIPLQEREDWLSAWAEGGEMPISMVEFGTPMDCTFRRGR
                     HGFESNITSEPLLTEYAAIYFGTDAYAAEEPKYRQYLHGLFRSGTLYESSENRLDEYA
                     NNHKIQQLYRINTWRSWRSAGL"
     gene            complement(1422..>2029)
                     /locus_tag="VIL39_02660"
     CDS             complement(1422..>2029)
                     /locus_tag="VIL39_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY5480357.1"
                     /translation="SFEGSSSGYPAQVPELAADSDWVTKSVELKVPKGATRLNIQPAM
                     FHCTGVFEIADLTLTPHLAATTQLADAGLPAGVSLNWDQVSITTVNAKRAQVSLDGIW
                     HFIPAAEGTAEVPKMGWAYIKVPGSWQGSRGRSADFVARGGGPQWDLYDGARVARAWY
                     KRQVPIPAEWQGRAISLRFDRVCTDAIVYVNGTECGRIAWP"
BASE COUNT          374 a          664 c          571 g          420 t
ORIGIN      
        1 agacccgcgc tccgccaact gcgccacgta ttgatacgat agagttgctg gatcttgtgg
       61 ttgttggcgt actcatccag ccggttctcg gagctctcgt agagcgtccc gctgcggaac
      121 aggccgtgaa gatattgccg atactttggt tcctccgcag cataggcgtc cgtgccaaaa
      181 tagatggcgg cgtattccgt caacaaaggc tcgctggtga tattgctctc aaacccgtgg
      241 cgcccgcgcc ggaacgtgca gtccataggg gtgccgaatt cgaccatgga aatcggcatc
      301 tcgcctcctt ccgcccatgc gcttagccag tcttcgcgct cctggagcgg tatcatgtcc
      361 aagtagcagt tcatcgtgta gatgtcgccc gtgtaggccc ccgagtggct gtaataggcc
      421 ctggtttggt ctagcttctt gagcccatcg aacatctcct tgccgaaaag catgaggcgc
      481 tgccagcgct catcgctctg atcccagccg cgtcggccca ggtggcgcgg atccgcgtcc
      541 accgcgttat tgaagaaatt gaagccggcg atccatatga ctacggatgg atggttgtgg
      601 tagtggcgca tccacacggc ggcgcggtcg aaggcgcgct gccggttctg ctcccagaca
      661 aaccgaccct tcgaactcat gaggtacttg ttcgcgttga ggatataatg tgccaccagg
      721 tatcccttcc gatcggcgtc ttcgagattt cgccccgagt cggcggcatc gccccgggcg
      781 tccacattcc cctctcccat ctcggagaag atgtccccca tttgcatgcg cgacccgttg
      841 taaaagcaag gctgccgcag gtggatcacg gtgccattga ggtaaaattg ccggccttcg
      901 atccagaact cccggaagcc gaatttctgg ttatactcat catccaggcc cgcgcccttc
      961 acctttagcc gcagggtgta aagatcgggc tggcccacgt cccataggcg cgggtcggcc
     1021 cagggccacg agacagcaaa ggcctgcgtc cccgcgctaa tcaccaccgc gtccgccgcg
     1081 aagctcttct ccacctcgcc cttttcgttg agcatgtccg caacgacatg gaccgggccc
     1141 gcgtgcttga cgccggccaa ctccacgtcc agggagatct cttttttccg agttgaagtg
     1201 cgcacgaaga catcggttac ccgtgcttcc gaggaacggc tttccaagaa aacgctgccg
     1261 gtcaaacccc ccgactttag cgaggccgcc gtgtaggaga cgttcatgaa ggcattctgc
     1321 caaaaggtcc ccacctgttc cgcatcggcg atggctgcca ccagcactcg cacgtccgcc
     1381 gtttgaccgg ccgttaccgc tgacgtgatg tccacggagc ctcagggcca ggcgattctt
     1441 ccgcactccg tgccgttgac atagacaatc gcgtccgtgc acacgcggtc gaagcgcagg
     1501 ctgatcgcgc ggccttgcca ttctgccggg attggcacct gccgcttgta ccaggcgcgt
     1561 gccacccgcg ccccgtcata gaggtcccat tgcgggccgc cgccacgcgc tacgaaatcg
     1621 gcagacctgc cccgagagcc ttgccaactg cccggcacct taatatatgc ccaccccatc
     1681 tttgggactt ccgcggttcc ttcggctgcc ggtatgaaat gccagatgcc gtccaaggac
     1741 acctgtgccc gcttggcatt caccgttgtg atgctcacct ggtcccagtt caggctgacg
     1801 ccggccggaa gcccggcgtc tgccaactgc gtcgtcgccg ccaagtgggg cgtgagcgtg
     1861 aggtcggcga tctcgaagac gccggtgcaa tggaacatcg caggctgaat gttcaggcgg
     1921 gttgctccct taggcacctt cagctccaca gacttcgtca cccaatcgga gtcagccgct
     1981 aattcaggaa cctgtgctgg gtagccagat gagcttccct cgaaactca
//
LOCUS       DATCGS010000133         5051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_10640, whole genome shotgun sequence.
ACCESSION   DATCGS010000133 DATCGS010000000
VERSION     DATCGS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10640"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1297)
                     /gene="rlmN"
                     /locus_tag="VIL39_02665"
     CDS             complement(68..1297)
                     /gene="rlmN"
                     /locus_tag="VIL39_02665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5480358.1"
                     /translation="MLMDIQSQTAEDLEAQFRAWGQPAYRVAQLLEWLYVHRAANWEA
                     MTNLPRALRDELRKQYSLTALELIRKQGARDTTQKFLWRLTDQALIESVLIPANPALY
                     GEASDRHTLCVSTQVGCAYGCKFCASGLKGWKRNLRVEEIVGQVLAVERWNEDESKVQ
                     SPKSKVQSQERGAESQNPESSLEDHASRITHHVSPSLQPRLVSNLVIMGMGEPLANYE
                     NLLKALRILNAPWGGGIGARKITISTSGLAPQIRKLAEESLQFRLAVSLHGATDATRD
                     KIMPVNRKYPLRELTAACEYYQQNRRRMITFEYVLIAGVNDGLDQVKPLATLAQRLNA
                     KVNLIPYNQVEGLPWERPTEEAQEAFLKALEKQKVSATLRREKGGDIDAACGQLRLKT
                     ERELAKVKVPSEAREHP"
     gene            complement(1362..3539)
                     /gene="glgP"
                     /locus_tag="VIL39_02670"
     CDS             complement(1362..3539)
                     /gene="glgP"
                     /locus_tag="VIL39_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY5480359.1"
                     /translation="MTKKPLTSDELRELVAGLHKVARNLWWTWNQEAQEIFQELSPRG
                     WQDLYHNAVAILHEVSDYELRVRLQDPYFAGRVCEVLRLFEAYMTDKTTWAHEHAPAL
                     RANPVAYFSAEFGFHETLPIAAGGLGILAGDHAKSASDLGLGFVGISLFYREGYFQQA
                     IDANNWQTEYYTLLNPKILPIEPVLDAKGEPMVCRVDIGMNEVAFQAWRANVGRAVVY
                     LLDANRPENEQHFRDLTQRVYGGDSTMRIMQEILLGIGGVRLLRALGVQPSVFHMNEG
                     HAAFLTLELVREKMAAGKKLPEALSETKAECIFTTHTPVEAGHDRFSPDLMDYAMHRF
                     RTQIAAPFGEIMKLGRVNPQNAQEPFCMTVLALKLSRAANAVSELHGRVSRHMWHCLY
                     SEKPVEQVPVGHITNGIHLLGWMKGTVRRFWQRKLTTSGDAAVPAGFRAPGPDWATQV
                     NSPEFWQRMADPAFITDEEIWALRFKLRRELIEFARRRLLIQGQRLAHGDFIAFDQLL
                     NPDALTIGFARRFATYKRAPLIFQQLDNIVRLTRDKERPIQFVFAGKAHPRDDDGKRY
                     IQHIIHLSKYSDLQGHMVFIENYDVHVARQMVSGCDVWLNTPRRPLEASGTSGMKAGC
                     QGCLNFSILDGWWREGYDGTNGFAIGEDAHADSVEAQDRADSASLYKVLTQDVIPLFY
                     QRDAPGIPRQWIQRIRRAMVTLVPQFTTARMVREYTEKYYLPK"
     gene            complement(3688..4758)
                     /locus_tag="VIL39_02675"
     CDS             complement(3688..4758)
                     /locus_tag="VIL39_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY5480360.1"
                     /translation="MTEQLRLLEPIRIGDLQLANRIFMAPLTRCHASAGRVPNPLMRD
                     YYVQRASAGLILSEATVVTPGGVGYPDTPGIWSAEQVQGWKVITEAVHAAGGKMFLQL
                     WHVGRISDPSYFGGALPVAPSAIAARGHVSLLRPKRPFVTPRALETSEIPGIVEAYRK
                     GAQNAQAAGFDGVEVHGANGYLLDQFLQDSSNHRTDQYGGPIENRARLMLEVTDAAIA
                     VWGSARVAMHLAPRGDSHDMGDSNPAATFGYVARELGRRRIAFICARESVGPGRLGPQ
                     LKTLFGGTYVANEGHTRDTAEQLLKAGEADAVAFGRLFIANPDLPRRFALNASLNMPV
                     PETFYASGPVGYTDYPALERTA"
BASE COUNT          892 a         1650 c         1505 g         1004 t
ORIGIN      
        1 cgaaaagcgt cctgtagaaa ggcattcgaa gcgttgcgca cggacgtggt ttgccgcatg
       61 aaatgcctca cggatgctcg cgggcttcgc ttggcacctt aactttcgcc aactcacgct
      121 ccgtcttcaa tctcagttgt ccgcaagcgg cgtcaatgtc cccgcccttc tcgcggcgca
      181 gggtcgcgct gaccttctgt ttctccagcg ctttgagaaa ggcctcctgt gcttcctcgg
      241 ttggacgctc ccagggcaga ccctcgacct ggttgtaggg aatcaggttt accttcgcgt
      301 tgagccgctg tgccagcgtc gccagcggct tgacctggtc caacccgtcg ttcacccctg
      361 cgatcaagac gtactcaaac gtgatcatcc gcctccgatt ctgctgataa tactcgcagg
      421 cagccgttaa ttcgcgaagg gggtacttgc ggttgaccgg catgatcttg tcccgggttg
      481 cgtccgtcgc accgtgcaga gaaaccgcca gccggaattg cagcgactcc tccgccagct
      541 tgcggatttg cggcgcgagg ccgctcgtcg aaatcgtgat cttccgcgcg ccgattcccc
      601 cgccccaggg cgcattcaga atcctcagcg ctttgagcag gttctcgtaa ttcgccagcg
      661 gctcccccat ccccatgatc accaggttgc tgaccaggcg aggttgaagg gaaggtgaaa
      721 cgtgatgcgt gatgcgtgat gcgtgatcct caagactgga ttctgggttc tgactctcgg
      781 ctccacgctc ctgactttgg actttggact ttggactttg gactttagac tcatcctcgt
      841 tccatcgctc gaccgccagc acctggccga cgatttcttc cacgcgcagg ttccgtttcc
      901 agcccttgag cccgctggcg cagaatttgc agccgtaggc gcagccgacc tgggtggaga
      961 cgcacagtgt atgccggtca ctcgcttcgc cgtagagcgc cgggtttgcc gggatgagca
     1021 cactctcgat cagcgcctgg tcggtcagcc gccacaagaa cttttgcgtc gtatcgcgcg
     1081 ctccctgttt ccgaatcagc tccagcgcag tcagcgagta ctgtttccgc agttcgtccc
     1141 gtaacgccct gggcagattg gtcatcgcct cccagttcgc ggcgcggtgc acgtaaagcc
     1201 attcgagcag ttgggcgacc cggtaggccg gctggcccca cgctcggaac tgcgcctcca
     1261 gatcttcggc tgtctgtgat tgtatgtcca tcaacacatc aagaggttag cgcattctaa
     1321 ctcatttaac ccagtttacc ttttgacgga ccaacccgcc ttcactttgg cagataatac
     1381 ttctccgtgt attcccgcac catacgcgcg gtggtgaact gtggcaccag ggtcaccatc
     1441 gcgcggcgga ttctctgaat ccactgccgg gggatgcccg gcgcgtcgcg ctggtagaac
     1501 aacgggatca cgtcctgcgt cagaaccttg taaagactgg cgctgtccgc ccggtcttgt
     1561 gcctcgaccg aatcggcatg ggcatcctcg ccgatggcaa agccgtttgt gccgtcatag
     1621 ccctcgcgcc accagccgtc caggatgctg aaattcaggc agccctggca gccggccttc
     1681 atgccgctgg tgcccgaagc ctccaatggc cgccgcgggg tgttcagcca cacatcacag
     1741 ccggacacca tctgccgggc cacgtgcacg tcgtaattct caatgaatac catgtgccct
     1801 tgcaggtcgc tgtacttgct caggtgaatg atgtgctgga tgtagcgttt gccgtcgtca
     1861 tcccgcggat gcgccttgcc agcgaagacg aattgtatcg gccgctcctt atcccgcgtc
     1921 aggcgcacga tgttgtccaa ctgctggaag atcagcggcg cgcgcttgta ggtcgcaaac
     1981 cggcgcgcga agccgatggt cagcgcgtcc gggttcaaca gttggtcgaa cgcaatgaag
     2041 tcgccgtgcg ccagccgctg gccctggatc agcagccgcc gccgcgcaaa ctcgatgagt
     2101 tcccggcgca gcttgaaacg caaggcccag atctcctcgt cggtaatgaa ggccgggtcc
     2161 gccatgcgct gccagaactc gggcgaattg acctgtgtgg cccaatccgg cccgggcgcg
     2221 cgaaaaccgg ccgggaccgc cgcgtcgccg ctggtcgtca acttgcgctg ccagaaccgg
     2281 cgaaccgttc ccttcatcca ccccagcagg tggatgccgt tggtgatatg gccgaccggc
     2341 acctgctcca ccggcttttc ggaatacagg caatgccaca tgtgccggct cacgcggccg
     2401 tgcaactcgc tcacggcgtt ggccgcccgt gacagcttca gcgccagcac cgtcatgcag
     2461 aacggctcct gcgcgttctg cggattcacc cggcccagct tcatgatctc cccaaacggc
     2521 gcggcgattt gcgtccggaa ccggtgcatc gcgtagtcca tcaagtccgg gctgaagcgg
     2581 tcatggcccg cttccacggg cgtgtgggtg gtgaagatgc attccgcctt cgtttcgctg
     2641 agcgcctccg gcagcttctt gcccgccgcc atcttctcgc ggaccagctc cagcgtcaga
     2701 aacgccgcat gcccctcgtt catgtggaag accgacggct gcacccccaa tgcccgcagc
     2761 agccgcacgc ccccaatccc cagcagtatc tcctgcatga tccgcattgt gctgtcgccg
     2821 ccataaaccc gctgggtcag gtcgcgaaaa tgctgctcgt tctcggggcg gttcgcatcc
     2881 agcaaataga ccacggcccg gccaacgttc gcccgccacg cctggaacgc gacctcgttc
     2941 atcccgatgt ccacccggca caccatcggc tcgcccttcg cgtccagcac cggttcgatc
     3001 ggcaggatct tcgggttcag cagcgtgtag tattccgtct gccaattgtt cgcatcaatc
     3061 gcctgttgga aatagccttc ccggtagaac aggctgatgc ccacaaaacc gaggccgagg
     3121 tcgctcgctg acttcgcgtg gtcccccgcc aggatgccca acccccccgc tgcgatcggc
     3181 agcgtctcgt gaaagccgaa ctccgccgag aaatacgcca ccggattcgc ccgcaacgcc
     3241 ggcgcgtgct cgtgggccca ggtggtcttg tccgtcatat aggcttcgaa aagccgcagc
     3301 acctcacaca cccgcccggc gaaatacggg tcttgcaacc gcactcgcag ctcgtaatcc
     3361 gacacctcat gcagaatcgc caccgcgttg tggtaaagat cctgccagcc ccgcggggac
     3421 agttcctgga agatctcctg cgcttcctga ttccacgtcc accacaggtt ccgggccacc
     3481 ttgtggaggc ccgcaaccag ttccctcaat tcgtcgcttg tcagcggttt cttcgtcata
     3541 tattgccagt caacatctct tcaaaactct atttcgcagg tgatcaaccc cccaaaccgt
     3601 aatcatcgcc agcggacaca gcaaaccaaa tccgtttacc gcggtggagg gatgtgctga
     3661 gtcccttcgc agtctgcttg gatgcgatta cgcagttctc tccagggcgg gatagtccgt
     3721 gtagcccacg gggccagagg cgtagaaggt ctcgggcacg ggcatgttga gcgacgcgtt
     3781 caaggcgaat cgccgcggca gatcggggtt ggcgataaag agcctcccga atgcgaccgc
     3841 gtccgcctcg ccggccttta gcaactgctc ggcggtgtcg cgggtgtgcc cttcgttcgc
     3901 gacataggtc ccgccgaaca gggtcttgag ctgcggcccc agacgacccg gcccgacgga
     3961 ctcgcgcgcg cagatgaacg cgatgcgacg ccggccaagt tccctggcga cgtagccgaa
     4021 ggtggcggca gggtttgaat cccccatgtc atgggaatcg ccgcgcggcg ccaagtgcat
     4081 ggcgacccga gccgaacccc agacggcaat ggcggcgtcg gtgacctcga gcatgagccg
     4141 cgcgcgattt tcgatgggcc cgccatattg gtcggtgcga tggttgctgc tgtcttgcag
     4201 gaactggtcg aggaggtagc cattggcgcc atggacttcc actccatcga agccggcggc
     4261 ctgcgcgttt tgcgcgcctt tgcggtaggc ctcgacaatg ccgggaatct cgctggtttc
     4321 gagggcgcgc ggggtgacga acgggcgctt ggggcgcagc aagctgacgt gtccacgagc
     4381 cgcgatggca ctcggcgcaa ccggcagcgc gccgccgaag tagctgggat cggaaatgcg
     4441 gccgacgtgc cagagttgga ggaacatttt cccgcccgcc gcatgcacgg cctccgtgat
     4501 aaccttccag ccctggacct gctcggcgga ccagatgccg ggcgtgtccg ggtagcccac
     4561 gccgcccggg gtgaccaccg tggcctcgct gaggatcaaa ccggccgagg cgcgctgaac
     4621 gtagtagtcg cgcatgagcg ggttggggac gcggccggcg ctggcgtgac aacgcgtcag
     4681 cggcgccatg aagatgcggt tagcaagctg caagtcgccg atgcgaatcg gttcgaggag
     4741 tcgaagttgt tcagtcatgt tggttcaagt ttctgccatg tggcgtgata ctaaaccaca
     4801 gttcggggga aaaagcgagc gtgtgtgaag tcgctcaatt gcccaagatg ccagaagggg
     4861 gcgaaacgga cgcctgcggg cacgaacccc gcacctccgc cggatcggcc ccggaccagc
     4921 gccccaggca ctcctttact acaccgctac caaccagtta tctggctttg gggtagcctt
     4981 ggggtagcct tggggtagcc ttggggtagc cttggggtag ccttggggta gccttggggt
     5041 agccttgggg t
//
LOCUS       DATCGS010000134         4481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_408095, whole genome shotgun sequence.
ACCESSION   DATCGS010000134 DATCGS010000000
VERSION     DATCGS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408095"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..935
                     /locus_tag="VIL39_02680"
     CDS             171..935
                     /locus_tag="VIL39_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY5480361.1"
                     /translation="MFYKVIACEIAFREFCYAAARSPNLIDLEFLTQGYHDIPVTGRG
                     EVQKRIDAVPVGRYDAILLGYGLCSNILTGLTTAHTPLVIPRAHDCITFFLGSKERYH
                     ECFHAKPGTYYYTSGWLECAKRRGSSGPIWGGASLPASANTSLKATYEQWVQKYGEDQ
                     AKYLLEEMSRWTDSYSHGTLITLDFVKHLKLQEQVRQICAEHGWQYDELQGDLGLFHK
                     LLDGEWPEPDFLVVRPGQKVVATNDELVIGLEPAGT"
     gene            complement(1023..3122)
                     /locus_tag="VIL39_02685"
     CDS             complement(1023..3122)
                     /locus_tag="VIL39_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5480362.1"
                     /translation="MRMRTSPLLPLLLAALLVAPLAMGVAPTPDELSAARQWAAARFQ
                     GSQEAPKAQPAPPSEPRAPYVTNAPFSFLYNGRSSAELLKEWPLERNSRESSPTQTEH
                     TLTWTDLHTGLQVRCVAVEYHDFPTVEWTLYFKNTGTNDTPILENIQALELGLERGTN
                     GEFLLHHNIGSPADGNDYGPLETPLGPKAHKRIGGNGGRPTNADWSYFNLEWPGQGLI
                     VAVGWPGQWAAELARGEGRGLHLRAGQELTHFKLLPGEEVRSPLIALQFWQGDWIRAQ
                     NVWRRWMMAHSMPKPGGKLPPPQLVASSSRQYEEMIKANETNQVMFINRYLEEHIKLD
                     YWWMDAGWYNHHGGGWPRVGTWEVDTNRFPRGLRAVSDYAHKNGMRILVWFEPERVTA
                     DTWLTRNHPEWVLGGAKGGLLNLGNEEARIWLTEHIDRLLTEQGIDLYRQDFNMDPLS
                     AWRSNDAPDRQGITEIKHVTGYLAYWDELRRRHPNLLIDSCASGGRRNDLETMRRAVP
                     LWRSDYAYEAIGHQCMTYGISLWLPYHGTGTLAARNAPYYGSGFTPVEPYAFWSNVSP
                     SLGLGIDMRVREIDYAALRRLIGQWRELSPNYYGDFYPLTRWSRDDQVWIAWQFDRPE
                     QGEGMIQAFRRHNSSYESACLTLRGLEPKARYAVINLDAPAARQELTGSELMERGLRV
                     DIPTRPGAALFTYRKIK"
     gene            3597..>4481
                     /locus_tag="VIL39_02690"
     CDS             3597..>4481
                     /locus_tag="VIL39_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5480363.1"
                     /translation="MKRSAFIGCLTTSLILVAGDASLLQFSAAAQTATAAAPGTLTVM
                     TYNLKFASTSPPNAWPQRRPLMAELIEKLAPDVFGTQEGLYGQLQDLAADLPAFQWIG
                     MGRDGGSRGEFSAVFYRTARLEPLAFDHFWLSDTPEVIGSKTWGPKLARMVTWVKFRD
                     RQTKREFIFVNTHFDHQVQAAREKSARLIRERVTAFDAKLPVLLVGDFNSAAGSNQAY
                     AILTEDKFLTDTWTTARERVNEGIGTLNGFKALQKDGPRIDWILGRGAVTADRIEIVT
                     FSRDGHFPSDHCPVVAKLL"
BASE COUNT          836 a         1393 c         1398 g          854 t
ORIGIN      
        1 tgaccacgga tgagcccgag agcaatggcg tcattgcctg ggccaagact tacaaaggcg
       61 cgcgggtggt ttatatccag tccggccacg accacttcac ctacgagaat cccaactacc
      121 agcaactcct ccggcaggcc attcgctgga cggcgcgcaa gaactgaccc atgttttaca
      181 aggtcattgc ctgcgaaatc gcctttcgcg aattctgcta cgccgctgcg cggtcgccga
      241 acctgattga cctggagttt cttactcagg gataccacga catccccgtc acgggtcgcg
      301 gggaggttca gaagcgcatt gatgccgtgc ccgtcggcag gtatgacgcc atcctgctcg
      361 gctacgggtt atgcagcaac atccttacgg ggctgacgac ggcgcacacg ccgctggtga
      421 tcccgcgcgc gcatgactgc attaccttct ttctcgggtc gaaggagcgc taccacgagt
      481 gcttccacgc gaagcccggc acgtattact acacgtcggg ctggttggag tgcgccaaac
      541 gtcgtggctc cagcggaccc atctggggcg gcgcctccct gcccgccagc gccaacacga
      601 gcctcaaggc gacctacgag cagtgggtgc agaaatacgg cgaggaccag gccaagtatc
      661 tgctggagga gatgagccgc tggaccgact cctactcgca cgggacgctg atcacgctcg
      721 acttcgtcaa gcacctcaag ctgcaagagc aggtgcggca gatttgcgcg gagcacggct
      781 ggcaatacga cgaactccaa ggcgacctcg gccttttcca caagctgctg gacggtgaat
      841 ggccggagcc ggatttcctg gtcgtgcgcc ccgggcagaa ggtcgttgcc accaatgacg
      901 agctggtgat cggcctcgag ccagccggga cctgaaccat tggaacagcg aagacgcgga
      961 ggcgcgaagt cgggggcggg gacgccccta ttctttcacc ttgctccagg agcagttcgg
     1021 cttcatttga tcttccggta ggtgaatagc gccgcaccgg gccgggtggg aatatccacc
     1081 cgcaggcccc tctccatcag ttcgctgccg gtgagttcct gccgggcggc gggggcgtcc
     1141 aggttaatca cggcgtagcg tgctttaggt tccagccctc gcagtgtcaa gcaggcggat
     1201 tcgtaggagc tgttatggcg gcgaaaggcc tggatcatgc cttcgccctg ctccgggcgg
     1261 tcgaattgcc aggcgatcca gacttggtcg tcgcggctcc atcgcgtgag cggatagaag
     1321 tcgccgtagt agttcgggct cagctcccgc cactggccga tgagtcggcg cagggcggcg
     1381 taatcaatct cccgcacgcg catgtcgatg cctaagccga ggctgggact cacgttgctc
     1441 cagaacgcat aaggctcgac cggcgtgaac ccgctgccgt agtaaggcgc gttgcgcgcc
     1501 gccagggtgc cggtgccgtg atacgggagc cagagggaga tgccgtaagt catgcactgg
     1561 tggccaatgg cttcatacgc atagtcgctg cgccagagcg gcacggcgcg gcgcatggtc
     1621 tccaggtcgt tgcgcctgcc gccggaagcg cacgagtcaa tcagcaggtt cggatgccgc
     1681 cgccgcaact catcccagta agcaagatag ccggtgacgt gcttgatttc agtaatgccc
     1741 tggcggtcag gcgcgtcatt gctgcgccag gcgctgagcg ggtccatgtt gaagtcctgc
     1801 cgatagaggt caatgccttg ctcggtcaaa agccggtcaa tgtgctcggt tagccagatg
     1861 cgcgcttcct cattgcccag gttcaacaag ccgccttttg cgccgccgag gacccattcg
     1921 ggatggttcc gggtcagcca ggtgtcggcg gtgacgcgct cgggctcgaa ccagaccagg
     1981 attctcatcc cgttcttatg cgcgtaatcg gacaccgcac gcaagccacg tgggaaacgg
     2041 ttggtgtcca cctcccaggt tcccacccgc ggccagccgc cgccgtggtg gttgtaccag
     2101 cccgcgtcca tccaccagta atcgagcttg atgtgttctt cgagatagcg gttgatgaac
     2161 atgacttggt tcgtctcgtt ggccttgatc atctcctcgt attgccgcga actggaggca
     2221 acgagctgcg gtgggggcag tttgccgccc ggtttgggca tcgaatgcgc catcatccac
     2281 cggcgccaga cattctgcgc gcgaatccag tcgccctgcc agaactgcaa ggcgatcagt
     2341 gggctgcgga cctcttcgcc gggaagcagc ttgaagtgag tcagttcctg gcccgcgcgc
     2401 aggtgcaagc cgcgcccctc gccacgcgcc aactccgccg cccactggcc gggccagccg
     2461 acggcgacga tgaggccctg gccgggccat tcgaggttga aataggacca gtcggcgttg
     2521 gtcggccggc cgccgttacc gccgatgcgc ttatgagcct ttggtccgag cggcgtttcc
     2581 agcgggccgt agtcgttgcc gtcagcgggt gagccgatgt tgtgatgcag caggaactcg
     2641 ccgttggtgc cacgttccaa cccgagttcc agcgcttgga tgttttcgag gatcggcgtg
     2701 tcgttggtgc cggtgttctt gaaatagagc gtccactcga cggtcgggaa gtcgtgatac
     2761 tcgacggcga cgcagcggac ctgcaacccg gtatgcaggt cggtccaggt caaggtatgc
     2821 tcggtctggg tggggctgct ctccctggag tttcgctcca acggccactc cttcagcaac
     2881 tcggcagatg aacggccatt ataaaggaaa gagaacggtg cgttggtcac gtatggcgcg
     2941 cgtggctcgc tgggcggggc tggttgggcc ttcggagctt cctggcttcc ctggaacctg
     3001 gcggcagccc attggcgggc cgccgagagt tcatcaggtg ttggcgccac ccccatcgcc
     3061 agcggcgcga ccaggagggc ggcaagaagc aggggaagca gaggcgaggt gcgcatcctc
     3121 atagagcttg gattcgatgt tatctgagac gtgacaattc aatgcaacca cagatggacc
     3181 cagatgaaca gcgataagaa ctattccaaa accgcttttc catgtcgaga acccatccgc
     3241 cgaacagaga cccaggggcg catcctgaca ccaccaccgg aacgccggat tcatccggca
     3301 gctccagtgc ggctcgccgg cctgccggat gaatccggcg ttccgctagg catggccacc
     3361 tgtttttgtg gtggtgtcag gatcccgaga cccaggaggt gtatcttgcc ggcaccactc
     3421 ggactgtggt gctcttgctg gcataatgtt cttcatcttc atttcaccct ctataggtgc
     3481 acaaaacgcg ttacttgagc cacaagccat ctcggccatt ccagaccacg cctggcggcg
     3541 actcgttctc gtgcgaagga cttgcacgcg gatggtgatt cactagaatc cgcgccatga
     3601 aacgaagcgc attcattggt tgcctcacca ccagcttgat cctcgtcgct ggtgatgcca
     3661 gcctcctgca attcagcgcc gcggcacaaa cggcaaccgc cgccgcgccg ggcacgttga
     3721 cggtgatgac ttacaatctg aagttcgcca gcaccagccc tccgaacgcc tggccgcagc
     3781 gccgcccgct tatggctgaa ctcatcgaaa agctcgcgcc cgatgtcttc ggcacgcaag
     3841 aggggcttta cggccagctt caagaccttg ccgcggacct gccggcgttc cagtggatcg
     3901 gcatggggcg agatggcggc agccggggcg agttctcggc ggtgttctat cgcaccgcgc
     3961 gtcttgaacc gcttgcgttc gatcacttct ggctgtcgga cacgccggaa gtcatcggct
     4021 cgaagacctg ggggccaaag ctggcccgca tggtgacgtg ggtgaagttc cgggatcgcc
     4081 agaccaaacg ggagttcatc ttcgtcaaca cccattttga tcaccaggtc caggcggccc
     4141 gcgaaaagag cgcccggctc atccgcgaac gcgtcacagc gttcgatgcc aaactgccgg
     4201 tgctgctcgt cggcgatttc aactccgccg ccggcagcaa ccaggcctac gcgattctga
     4261 ccgaggacaa gtttctcact gacacctgga cgaccgcgcg tgagcgagtg aacgaaggca
     4321 tcggaaccct caacggattc aaagctcttc agaaagacgg accgcgcatt gactggattc
     4381 taggccgtgg cgccgtgacg gcggaccgca tcgagattgt gaccttctcg cgcgacggcc
     4441 acttcccgag cgaccactgc ccggtggtgg cgaagctgct g
//
LOCUS       DATCGS010000135         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_705229, whole genome shotgun sequence.
ACCESSION   DATCGS010000135 DATCGS010000000
VERSION     DATCGS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705229"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..840
                     /locus_tag="VIL39_02695"
     CDS             475..840
                     /locus_tag="VIL39_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480364.1"
                     /translation="MSFNPTRRRVILCGIAAEVMALGFAGGAAQAKLTERQAAARPPN
                     ILFVLADQWRFEAFGYAGNPDVKTPKNLPKYSRLQGRLDATLNRKLEERHDNFLPGDA
                     YIKQWGYKVDANGSVPYKP"
     gene            complement(989..2269)
                     /gene="argA"
                     /locus_tag="VIL39_02700"
     CDS             complement(989..2269)
                     /gene="argA"
                     /locus_tag="VIL39_02700"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY5480365.1"
                     /translation="MKLTDLRGILQYIPQFREKTFIINVDGAIVTDENFANILVDIAV
                     LRSLSIRVVLVHGASAQIKTLAAAQGITSSNLDGTGVTDEATLRLALTAANRLTHEVL
                     EGLSAIDLRAACTNAVIAHPVGILHGVDQLFTGKVERVDVEMLQQLLEHGIVPVLPPL
                     GFDGEGKTYRVNSDGVALAVAEALKATKLMFITTYDGIRVGERVIRQMPVSDLESVLT
                     LQRMDVHPDMLSKAIHAAAACKAGVPRVHVINGSVEEGLLAEVFTNEGVGTLIYGNEF
                     QQIRRALKKDVRSILNLTKRSVESEELVKRTRSTIEKQLGDYYIFEIDKNPVACVALH
                     SWPEQGKGELACLYVSPAHENQGIGRKLVQYVENKAREMGLNELIALSTQAFTYFQSK
                     SGFVEGTPDDLPPARREKYDQSGRNSKVLVKKLK"
     gene            complement(2293..>2911)
                     /locus_tag="VIL39_02705"
     CDS             complement(2293..>2911)
                     /locus_tag="VIL39_02705"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY5480366.1"
                     /translation="NPTGTVARREDLARFAAAVPENVLLALDEAYIEFLDEPPDLVPE
                     IRSGRKANLLLMRTFSKIYGLAGLRIGYGVGHPDLIAEFEKVRQPFNINAIAQAGALA
                     ALDDTGHTEKTRRGNSRGLRFYARAFRKLGLEFVPSSANFILVRVGEGQRVFNEMQKL
                     GVIVRPMGGYQLPEWVRISIGTPKENQRCLEALTTALAKPQPSSL"
BASE COUNT          534 a          816 c          979 g          582 t
ORIGIN      
        1 tccgaacgct ggtgaaggaa tcttcgcacg ccgctgaaat cgcaaagtgc tttagttcct
       61 tccagatcgg ccacacccga agtttggaga tcatcgcatc catgaaaaga agatggctgt
      121 tttacctaac catcggcgcg caatgaagct gagtcaaatg gatttcggtg atgtttgtct
      181 cggccggctt cttagtttct gctttccaaa cggaccggat aggatagaat cagtttgcat
      241 gttcaagggt cccacccgaa gaagtgatgg gccgtttagg gaggcgccag ccgcagcccc
      301 gccccgccag acgccgccgc aggcggaaga atgaacgttg gactctcaat tccaatgctc
      361 cgacccgatg cagtcgcagc ggcccagcgg agtgagagtc ctgcgcacgg aaggggaccg
      421 agtagcgctg gcggtagatt ccggcactta ctggttcgca accggaggaa gccggtgagc
      481 ttcaacccca cacggcggcg tgtcatcctg tgtggaatag cggcggaggt aatggcgctg
      541 ggtttcgcgg gtggcgcagc ccaggccaag ctgactgaac gccaagccgc cgctcgcccg
      601 ccgaacatcc tttttgtcct cgccgaccag tggcggtttg aagcgttcgg ttacgcgggc
      661 aacccggatg tcaagacgcc taaaaacctg ccaaagtact ccaggttgca gggcagactt
      721 gatgccacgc tcaaccgcaa actcgaggaa cggcacgaca actttctgcc tggcgatgcc
      781 tacatcaagc agtggggcta caaggtggac gccaacggct cggtgcctta caagccctga
      841 tcaacgaatt ggttgaggta gctgcggcaa ggtcggcgac ttgaaatgac cggcttaaag
      901 ccctgctggc cagtgagcgc tggccagacc tcaagcggga ctcctcaggc tgccttttcc
      961 gtgggtggaa tgattggcgg ccgcagcgct acttgagctt cttgacgagc accttggagt
     1021 tgcggccgct ttggtcgtat ttctcgcgcc gggccggcgg caggtcatcc ggcgtgccct
     1081 cgacgaagcc gctcttggac tggaaatagg tgaaggcttg cgttgagagg gcgatgagtt
     1141 cgttgaggcc catctcgcgg gccttgtttt caacgtactg gacgagcttg cggccgatgc
     1201 cttggttttc atgggccggg ctgacgtaga ggcaggcgag ctcgcctttg ccctgctcgg
     1261 gccaggagtg cagggccaca caggcgacgg ggttcttgtc gatctcgaag atgtaatagt
     1321 cgccgagctg cttctcgatg gtggaacggg tgcgcttgac cagttcctcg ctctcgacgg
     1381 agcgcttggt gaggttgagg atgctgcgga catctttctt gagcgcacgg cgtatctgct
     1441 ggaattcgtt gccgtagatg agggtgccaa cgccttcgtt ggtgaagacc tcggcgagca
     1501 gcccctcttc gacggagccg ttgatgacat ggacgcgtgg cacgccggcc ttgcaggcag
     1561 cggcggcgtg gatggccttg gagagcatgt ccggatgcac gtccattctc tggagtgtga
     1621 gcacggactc caggtcggat acaggcatct ggcggatgac gcgttcaccg acgcggatgc
     1681 catcgtatgt ggtgatgaac atcagcttcg tggctttgag cgcctcggcc accgccaggg
     1741 cgacgccatc ggaattgacc cggtaggtct tgccctcgcc gtcaaagccc agtggcggca
     1801 ggacggggac gatgccatgt tcgagcagtt gctggagcat ctcgacgtcc acccgctcga
     1861 ccttgccggt gaacagttgg tccaccccgt gcaggatgcc gacggggtgg gcaatgacgg
     1921 cattagtgca tgcggcgcgc aggtcaatcg ccgagaggcc ttcgaggacc tcgtgcgtca
     1981 agcggttggc ggcggtcagg gcgagccgga gcgtggcttc gtcggtaacg ccggtgccgt
     2041 cgaggttgga cgacgtgatg ccttgcgctg cggccagggt tttgatctgc gcggaggcac
     2101 cgtggacgag cacgacgcgg atactcagcg agcgcagcac ggcaatgtcc accagaatgt
     2161 tggcgaagtt ctcgtcggtg acgatggcgc cgtccacgtt gatgatgaac gtcttctcgc
     2221 ggaattgggg gatgtactgc aggatgcccc gcaggtcggt gagtttcacg ttagataaag
     2281 cttaggactc agttataggg aggagggttg gggtttcgcg agggctgtgg tgagggcttc
     2341 caggcaccgc tggttttctt tgggtgtgcc gatggagatg cggacccatt cgggcaactg
     2401 gtagccgccc atgggccgga cgatcactcc cagcttctgc atttcgttga acacccgctg
     2461 gccctcgccg acccggacca ggatgaagtt ggccgaggaa gggacgaatt ccagacccag
     2521 cttgcggaac gcgcgcgcat aaaaccgcag cccgcgcgag ttgccccgcc gggtcttctc
     2581 cgtgtgcccg gtgtcgtcca gcgccgctag cgcgccggct tgggcaatgg cgttgatatt
     2641 gaagggctgg cggactttct caaactccgc gatcaagtcg gggtggccga cgccgtagcc
     2701 aatgcgcaag ccggccaggc cgtagatctt ggagaaagtc cgcatcagga gcaggttggc
     2761 cttccgtccg ctgcgaatct cgggaaccag gtcaggtggc tcgtccagga actcgatgta
     2821 ggcttcgtcc agggcgagca ggacgttttc gggcacggcg gcggcaaagc gggccaggtc
     2881 ttcccggcgg gcgacggtgc cggtcgggtt g
//
LOCUS       DATCGS010000136         5471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_208207, whole genome shotgun sequence.
ACCESSION   DATCGS010000136 DATCGS010000000
VERSION     DATCGS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208207"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VIL39_02710"
     CDS             complement(<1..240)
                     /locus_tag="VIL39_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5480367.1"
                     /translation="MLTVSNIRKTFNPGTVNEVRALRGVSLEIPEGSFLVILGGNGSG
                     KSTLLNAIAGTFQLDAGEIHLDGNRLTRWPEHRRAR"
     gene            complement(256..1194)
                     /locus_tag="VIL39_02715"
     CDS             complement(256..1194)
                     /locus_tag="VIL39_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480368.1"
                     /translation="MNLLLGAITIGLILSLLALGIYISYRVFDVADITVDGSITLGAA
                     ICAVLIIKGVHPLFATFGGFAGGLVAGAITGILQTKFKINALLSGILVMTGLYSVNLR
                     IMGRSNVPLLSGESFITLAERLGRRWFGRPEVNLLGWMISVRDLVTLLAVLAMIVLAC
                     LALYWFFRTEMGTAMRAAGDNPQMIRALGVSVERYRIMGLALANGLVALSGALLAQYQ
                     GFADAQMGIGMVVWGLASVIIGESLTGSRSLGLAIYGAIAGSVLFRMLVAIALRWGLN
                     PTDLKLVTALFVFAALVMPQMLRRRRTSVPAASSQP"
     gene            complement(1293..2522)
                     /locus_tag="VIL39_02720"
     CDS             complement(1293..2522)
                     /locus_tag="VIL39_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5480369.1"
                     /translation="MQITRTARAEQFEIKLKGRMDASWSDHVARALAECVQSGQHVIA
                     VDMSEVDYMSSAGIRILVMYARQLKSIQGRLFVINASNMVRKVLELSGLDSLLHAPAA
                     PAPAAVADGLAAHIVLPQPGATAEVIELDAQAVLRVYWPGKPASWLAGSAQSGTPVTV
                     EFPASTMGIGLGALGDGDSDGEQRFGEFLAASGAAVCQPADGANKPDYMLLEGALTPS
                     MKVAYGMLGCGGFRRLLRFDKGPQQASLPFSAVVKACLDASGGDAIGLVMVAETTALI
                     GASLQKMPSAGAPPNIFAFPGVRDWLSFTAEAAFANTTSLIVGFAASQARGPGLHLLK
                     PLVPSGELHGHFHAAAFPYQPLRKGKVDLGETARPLFDSGQILGLLHLLNDWREINGA
                     GESRFLRGACWCSPLVT"
     gene            complement(2537..4591)
                     /locus_tag="VIL39_02725"
     CDS             complement(2537..4591)
                     /locus_tag="VIL39_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5480370.1"
                     /translation="MIMPTLKRLLLSLALLGAASLVLLFSDLHSRKGAQGKNNGSQPP
                     RQVALLKHSSTMLLDEVERGMVEQLAAAGYQEGGRLALQRFNAEGDLPTANAIAQRMT
                     DGSFEMAITISTLSLQCVANANKDGRTIHVFAGVTDPAGAGVGIQQMNSTNKPPWLAG
                     LGTFQPVEQILREAKRFWPGLKVVGVVWNPAERNSEVCTIKAREVCRALGLQLLEATV
                     DQSKDVREAAASLVARGVQAIWTGADVTVMNATAALCETALKAKIPVFSNTSGQVREG
                     TLFDLGANYVEVGSSLGALAASILDGGNPATMVITNFMPERVMLNKQVLKQLRDPWRF
                     PDDIIARAELILGEDGRVEKDASKTGKAAVLAMQTPVVADTAARKPLAKRWRIQQAIY
                     NESVMVEEAMRGFQAGLKEAGLHEGTDYTLHTLSAQGDMATLGTLFDSAKTAGTDIYA
                     VYGTPTLQSAIRKVQDTPVVFTVVADPFVAGAGRTDTEHLPNVTGVYTQGPYREMAEL
                     LREHFPNIKRVGTLFCPAEANSVANKDVFVREATRCGLTVETMAANGPSELPDAAMAL
                     CGRQIDAVVQVIDNLSAAGFPTIARAGAQARLPVFACQGGAVKEGATLALARDYYDAG
                     REMALKAAQVMRGESPARIPFSPPTKLEMLVNLKNAGESRLVIPEALLRQAQQVTTPT
                     TP"
     gene            complement(4755..>5471)
                     /locus_tag="VIL39_02730"
     CDS             complement(4755..>5471)
                     /locus_tag="VIL39_02730"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5480371.1"
                     /translation="FPPFPEHTEFDIYAELLAAKEVGGDLYDYFLLDDHRLFFSIGDV
                     SGKGVPAALFMAVTKTLMKGVAEQHLSPSQVLAKVNHELYVDNDQMMFVTLCCGVLDL
                     NTGEVHYTNAGHPPPLRLRQGTGANWVDLPRGSVLGIDEEPAYETKTLRLEPMDTLLL
                     YTDGVTEAMNAGQELFGDDRLRSLAEAQTGAAPKELVEALFGAVRHFAGGEEQSDDIT
                     VLALRYRGRSQDNPGNKTKS"
BASE COUNT         1043 a         1753 c         1681 g          994 t
ORIGIN      
        1 ccgcgcgcgc cggtgctccg gccagcgcgt cagccggttg ccgtcgaggt gaatttcgcc
       61 cgcgtccaac tggaaggtgc cggcgatcgc gttgagcagg gtggatttgc cggagccgtt
      121 gccgccgagg atgaccagga acgatccttc aggaatctcc aggctgaccc cgcgcaaggc
      181 gcggacttca ttgacagtgc cgggattaaa ggtcttgcgg atattgctga cagtaagcat
      241 ggcgggcctt aggggttacg gctgcgaaga agcggcgggg acggatgttc ttcgccgccg
      301 caacatctgc ggcatcacca gcgccgcaaa cacaaacaag gcggtgacca gtttgaggtc
      361 ggtgggattg aggccccaac gcagcgcgat ggcgacgagc atccggaaca gcacggagcc
      421 ggcgatcgcg ccatagatgg ccaaccccag gctgcgcgaa ccggtcaggg attcaccaat
      481 gatgacgctg gccagacccc agacgaccat gccgatgccc atctgggcgt cggcaaaacc
      541 ctgatactgg gccagcagcg cgccggaaag ggcaacgagg ccgttggcga gcgcgagccc
      601 cataatgcgg tagcgttcga cgctgacgcc caaggcgcga atcatttgcg gattgtcgcc
      661 ggcggcgcgc atggcggtgc ccatttcagt gcggaagaac caatacaggg ccaggcaggc
      721 gagcacgatc atggccagca ccgccagcag tgtcaccaga tcgcgcacgc tgatcatcca
      781 gcccagcagg ttgacttccg gccggccaaa ccacctgcgg cccagccgct cggccagggt
      841 gatgaaactc tcgcccgaca acagcggcac gttgcttctc cccataatgc gcaagttcac
      901 cgaatacagg cccgtcatga ccagaatccc cgacaacagg gcgttgatct tgaatttggt
      961 ctgcaaaatg ccggttatgg cgcctgccac caacccgccg gcaaatcccc cgaacgtggc
     1021 gaacagcgga tgaacgccct tgataatcag cacggcgcag atagccgcgc ccagcgtgat
     1081 cgaaccgtcc acggtgatgt cggcaacgtc aaagacccgg tagctgatat agattcccaa
     1141 cgcaagcagc gacaggatca gaccgatggt gattgctccg agtaagagat tcatggcggg
     1201 ttcaggttca agacaaaatg gatcgggggc attgcggctg cgccacagaa cgccggtgat
     1261 gcgggattga tgcggagtga cggcatatag cgctaggtga ccaggggcga gcaccaacag
     1321 gcgccgcgca ggaaacggct ctcccccgcc ccgttgattt cgcgccagtc gttcagcaga
     1381 tgcagcaaac caaggatctg cccgctatca aacaacggac gggcggtttc tcccagatcc
     1441 accttgcctt tgcgcagggg ctggtaagga aacgccgccg cgtggaaatg accgtgcagt
     1501 tcaccggagg gcacgagcgg tttgagcaaa tggagccccg gtccgcgcgc ctggctggcg
     1561 gcaaaaccaa cgatcaggct ggtggtattc gcaaacgccg cttcggcggt aaagctcagc
     1621 cagtcccgca ccccgggaaa ggcaaagatg ttcggcgggg ctcccgccga tggcatcttc
     1681 tgcaaggacg cgccgatcag agcggttgtt tccgccacca ttaccaggcc gatggcatcg
     1741 ccaccgctgg catccagaca ggctttcacc accgcgctga acggcaggct cgcttgctga
     1801 ggacctttgt caaaccgcag cagacgccgg aaaccgccgc agcctaacat gccgtaggcc
     1861 accttcatgg atggcgtcaa tgccccttcc aatagcatgt aatccggttt gtttgcgcca
     1921 tcggccggct ggcagacggc ggcgccgctc gcagccagaa attccccaaa tcgctgttcc
     1981 ccgtccgaat ctccgtcgcc cagggcgccg agaccgatgc ccatggtact ggcagggaat
     2041 tccaccgtga cgggagtccc ggattgcgca ctaccggcga gccatgacgc gggcttcccc
     2101 ggccaataca ctcggagcac ggcctgggca tcgagttcga taacctctgc ggtcgccccg
     2161 ggctgcggca gcacgatatg cgccgccaat ccgtcggcaa cggccgcggg cgccggggcg
     2221 gcgggggcgt gcaggagcga atcaagtccg gaaagttcca acaccttccg gaccatgttg
     2281 gatgcgttga taacgaaaag ccgcccctgg atggatttga gctgccgcgc atacatgacc
     2341 aagatgcgaa tccccgcgga actcatgtag tccacttcgg acatgtccac ggcgatgacg
     2401 tgctggccag attgcacgca ttccgccagg gcgcgggcca cgtggtcgct ccaactggca
     2461 tccatccggc ccttcagttt gatctcgaat tgttcagcgc gagcagtgcg ggtgatttgc
     2521 atggtcaggc acagggttaa ggggtggtgg gtgtggtaac ctgctgcgct tgccggagca
     2581 gcgcctcggg aatgaccaac cggctttccc cggcattctt aaggttcacg agcatttcca
     2641 gtttcgtggg cggtgagaac ggtatccgcg ccggactttc gccgcgcatc acctgcgcgg
     2701 ctttgagcgc catttcacgc ccggcgtcgt agtaatcgcg cgccagcgcc agcgtggcac
     2761 cctctttgac cgccccgccc tggcaggcaa aaacaggcag ccgggcctgc gccccggcgc
     2821 gggcgatggt gggaaagcca gcggcgctaa ggttgtcaat cacctggacc acggcgtcaa
     2881 tctggcggcc acacagcgcc atggccgcgt ctggcagttc gctggggcca ttggcggcca
     2941 tggtctccac cgtcaagccg caccgggtag cctcgcgcac aaacacgtcc ttgttggcca
     3001 ccgaattggc ctcggccgga caaaagagcg tgcccacccg cttgatattc gggaaatgct
     3061 cccgcagcag ctccgccatc tcacggtatg gaccctgggt gtacaccccg gtgacgttcg
     3121 gcaggtgttc cgtgtcggtc cttcccgcgc ccgccacaaa cgggtcggcc accactgtga
     3181 acaccacggg ggtatcctgc acttttcgga tcgcgctctg gagagttggc gtgccataga
     3241 cggcataaat gtcggtcccg gcggttttgg ccgagtcaaa cagcgtgccc agcgtggcca
     3301 tgtcgccttg ggcgctgagc gtatgcaggg tgtaatccgt cccctcgtgc agtccggcct
     3361 ctttcaggcc agcctgaaaa ccgcgcatgg cttcctcgac catgacggac tcattgtaga
     3421 tggcctgttg gattcgccag cgtttggcca gcggcttcct ggccgccgta tccgccacca
     3481 ccggggtctg catggccagc actgccgctt ttccggtctt ggacgcgtct ttctccaccc
     3541 ggccgtcctc gccaaggatc aattccgcgc gggcgataat atcgtcggga aaccgccagg
     3601 gatcgcgcaa ttgtttaagc acctgtttgt taagcatcac ccgctcaggc atgaagttgg
     3661 tgatgaccat tgtggcggga ttcccgccat cgaggatcga ggccgccagc gccccgaggc
     3721 tgctgcccac ctccacatag ttcgcgccca ggtcgaagag cgtgccttcg cgcacctgcc
     3781 cgctggtgtt ggagaacaca ggaattttag ccttgagcgc cgtctcacag agggcggcgg
     3841 tggcgttcat cacggtgacg tcggcacccg tccaaatagc ctgaaccccg cgggccacca
     3901 gcgaagcggc ggcttcccgc acgtccttgc tctggtccac cgtagcctcg agaagctgca
     3961 ggccgagcgc acggcagacc tcacgggcct tgatcgtgca aacttcggaa tttcgctccg
     4021 ccggattcca gaccacccca acgaccttca ggccgggcca aaaccgcttg gcctccctta
     4081 gaatctgctc gaccggctgg aatgttccca ggccggcgag ccagggcggc ttgttggtgg
     4141 agttcatttg ctggatgcca acgcccgcgc cggcgggatc ggtcaccccg gcgaacacat
     4201 ggatggtccg tccgtctttg ttggcgttgg ccacgcattg cagggagagc gtgctgatag
     4261 tgatggccat ctcgaaactg ccatccgtca tccgctgggc aatcgcgttg gcagtgggca
     4321 ggtcaccctc ggcattgaac cgctggagcg ccagacgccc gccttcctgg tagcctgcgg
     4381 cggccagttg ctccaccatc ccgcgctcca cttcatccaa cagcatggtg gaagagtgtt
     4441 tcaacaaggc cacctggcgc gggggctggc tcccgttgtt tttgccctgc gcccccttcc
     4501 gcgagtgcag gtcggagaaa agcaaaacca gcgaggccgc gcccagcaat gccagcgaca
     4561 gcaaaaggcg cttaagagtt ggcatgatca tgatgagggc gtaatggctt ggctctattg
     4621 gcggtaggcc acgactgacc ggcgggttga ttgcctgcaa tcagccccag gtgccacttc
     4681 tttgtgcggc ggcttccctt ccgcccgagg cggtgtcttt ttccggggat tagcggcctt
     4741 ggatttggtg gttttcacga cttggttttg ttcccagggt tgtcctggct ccgcccgcgg
     4801 tatcgcagcg ccagcacggt gatgtcatcg gattgctcct cgccgccggc aaaatgacgg
     4861 accgcgccaa acaacgcctc gaccagttcc ttcggcgcag cgcccgtctg ggcctctgcc
     4921 agggacctca gccggtcgtc gccgaaaagc tcctggcccg cattcatcgc ttcggtcacc
     4981 ccatcggtgt aaagtaacaa cgtatccatg ggctcaagcc tgagcgtttt ggtttcgtag
     5041 gcgggctcct cgtcgatgcc cagcaccgag cccctgggca ggtccaccca gttggcccct
     5101 gtgccctgcc gcagccgcaa gggcggcggg tggccggcat tggtgtaatg cacctcgccg
     5161 gtattcaaat caagcacgcc gcaacagagc gtgacaaaca tcatttggtc attgtccacg
     5221 taaagctcat ggttgacttt tgccagaacc tgcgacgggg acaaatgctg ctcggccacg
     5281 cctttcatca gcgtcttggt caccgccata aatagcgccg ccggcacgcc cttgccggag
     5341 acatcgccaa tcgagaagaa cagccggtgg tcgtcgagca ggaaatagtc gtacaaatcg
     5401 cccccgactt ccttggccgc cagcagctcg gcgtagatgt cgaactcggt gtgctccgga
     5461 aagggcggga a
//
LOCUS       DATCGS010000137         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_61956, whole genome shotgun sequence.
ACCESSION   DATCGS010000137 DATCGS010000000
VERSION     DATCGS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61956"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VIL39_02735"
     CDS             <1..1159
                     /locus_tag="VIL39_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480372.1"
                     /translation="APGNQNDDCDYSRFFGKGCIQRYNYYHGSTRQEIKTAHVDCLQT
                     FSNNGEIAMDLLFEYNTCFDFHQMCMVESAPHIGSVRGWTFRHNIVSPNSPTMRGGWG
                     PDIIQTPDVTIENCTISTVNWATIGLRGKESTGGLIRNNILCEAERAVDDTMDFTPAK
                     PVIEYNLTFKTAPVSGEHNLNGQDPLFVDAAKRDFRLRKGSPAIGAGKDEMTIGALEY
                     PNVYYVDPRHPAASDEPAWGYPGVPLASLAKACAIAQPGETIVLRGGVYREVLAPKSD
                     GVTVRAMKGEKVTISGADLIEGWKREVDGSWSAPLAPQPKKVLRDGQPWSEFRYDQAA
                     KRITVKAGGDPRLHVFETVMREQGIDLGGKKDAKIEGITVVDTLKAGSSNP"
     gene            complement(1133..1381)
                     /locus_tag="VIL39_02740"
     CDS             complement(1133..1381)
                     /locus_tag="VIL39_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5480373.1"
                     /translation="MQIAARILLIEDDTSLAANLCDVLIRGETGTGKELIARAIYQHS
                     PRAGLGCPGSPCAKNSSSLACIRFDIPPVPSRIRGARL"
     gene            complement(1435..1947)
                     /locus_tag="VIL39_02745"
                     /pseudo
     CDS             complement(1435..1947)
                     /locus_tag="VIL39_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1920..2651
                     /locus_tag="VIL39_02750"
     CDS             1920..2651
                     /locus_tag="VIL39_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEY5480374.1"
                     /translation="MNTGAAKAPWGSRIPAPHYRWLLFDADGTLFDYDGAERVALGQA
                     LGQIGVSFDPGFLATYRLINQTLWQGVEKGELTPGVVKVRRFELWLEAIQVAYSPAAF
                     SASYLECLANCSELLEDAAKVLGALSRKYRIAILTNGLKEVQRGRLARSVIRHHISDI
                     IISEEVGFAKPAKEYFETAFARLGHPSKHEVLMIGDSWTSDIQGAVQYGIDACWYNPG
                     RKPRPANYEITREIASLRELNDWLG"
     gene            complement(2754..>2859)
                     /locus_tag="VIL39_02755"
     CDS             complement(2754..>2859)
                     /locus_tag="VIL39_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEY5480375.1"
                     /translation="DVIWAAPYCPQWFVAMGKKPASCIYYQDVNREPM"
BASE COUNT          584 a          851 c          902 g          522 t
ORIGIN      
        1 cgccccgggc aaccaaaacg acgactgcga ttactcgcgg ttcttcggca agggctgcat
       61 ccaacggtac aactactatc acggcagcac ccggcaggag atcaaaaccg cccacgtcga
      121 ttgccttcag accttctcga acaacggcga gatcgcgatg gacctgctct tcgagtacaa
      181 tacctgtttt gactttcatc agatgtgcat ggtggagagt gctccgcaca tcggcagcgt
      241 gcggggctgg accttccggc acaacatcgt ctcgcccaat tcgccgacca tgcgaggggg
      301 ctggggtccg gacatcatcc agacgcccga cgtgacgatc gagaactgca ccatctcgac
      361 ggtgaattgg gccaccatcg gcctccgggg caaggaatcc actggcgggc tgatccgcaa
      421 taacattctc tgtgaggcgg agcgggccgt tgacgacacc atggatttca ccccggcaaa
      481 gccagtgatc gagtacaatc tgacgtttaa gactgcgccc gtttccggcg agcataacct
      541 caacggccag gacccgctct tcgtggatgc ggccaaacgt gatttccgtc tgcggaaggg
      601 cagcccggcc atcggagcag gcaaggacga gatgacgatc ggagccctgg agtaccccaa
      661 cgtctattac gtggacccgc ggcatccggc cgcctccgac gagccggcat ggggctatcc
      721 cggcgtgccg ctggcgagcc tggccaaggc gtgcgcgatc gcccaaccgg gagagacaat
      781 cgttctgcgg ggcggagtgt atcgcgaggt cctggccccg aaaagcgacg gcgtgaccgt
      841 ccgcgccatg aagggcgaga aggtgacgat cagcggggcg gacttgatcg aaggctggaa
      901 gcgcgaggtg gacggcagtt ggtcggcgcc cctggccccc cagccgaaga aggtcctgcg
      961 cgatgggcag ccgtggagcg agttcaggta tgaccaggca gccaagcgaa tcacggtgaa
     1021 ggcgggcggc gatcctcgcc tgcacgtctt cgagacagtc atgcgggagc agggcatcga
     1081 cctgggcggc aagaaagacg cgaagatcga ggggatcacg gtagttgaca cactaaaggc
     1141 gggctcctcg aatccgtgag gggacaggtg ggatgtcaaa tcggatgcaa gccaagctgc
     1201 ttgagttttt cgcgcaaggt gagccgggac agcccaagcc agcgcgcggg ctgtgctggt
     1261 agatggcgcg ggcaataagc tccttgcccg tccctgtttc gccccggatg agcacgtcgc
     1321 acaggtttgc ggccagactg gtgtcgtctt caatgagaag gattcgcgct gcaatttgca
     1381 ctcccggcag tgtccccgtt ttcgcgcggc aagacaaccc ccgaaggtcg tgccgtgacc
     1441 gggccgggtc tgatactgga gcatgccgcc gtgcttttct acgatgcgag cggcaatcgg
     1501 caagccgagg ccggtgccgg tttccttcgt gctgaagaat gggtcgaata agcgcttctc
     1561 gacttcggac ggaatgccgt tgccagtgtc gctcacttca agaaccacgg cgtcggtctc
     1621 gcgccccccg agcgaggcgc gtgccgcgcg cgcgcgaagc gtgaccgtgc ccgcgccggc
     1681 aatggcgtca atggcgttgc gaaccaggtt gatgagcacc tgctcttgcg gaagtcggct
     1741 gactgacaaa taaaaccgcc aagcgccgct gtgggatgga ccggaccagc cattccctgc
     1801 cgaacgacca aaccgaatct gataacttgg gcctggttgt cgcgctcgca gcttgtttcg
     1861 gcggcaattc gggtgttcaa tgggcgcatg agccttgttc cagaacttcg gcagagaccg
     1921 tgaatacagg agccgcgaaa gccccatggg gatctcgcat tccggcgccc cattaccgct
     1981 ggctgctgtt cgacgccgac ggaacgctct ttgactacga cggggcagag cgcgtggcgc
     2041 tcggacaagc cctcgggcag ataggagttt cattcgaccc cggcttcctg gccacctacc
     2101 ggctgatcaa ccagaccctg tggcaggggg tagagaaggg agaactcacg cccggcgtgg
     2161 tgaaagtgcg tcgcttcgag ctgtggctgg aagccatcca ggtcgcatat tcaccggccg
     2221 ctttcagtgc cagctatctt gagtgcctgg ccaactgctc ggagctgctg gaggatgctg
     2281 ccaaggtgct tggggcgttg agcaggaaat accggatagc gattctgacc aacgggctga
     2341 aggaggtcca gcgtggccgc ctggctcgct ctgtcatccg ccaccacatt tccgacatca
     2401 tcatctccga agaggttggg tttgccaaac cggccaaaga gtatttcgag acggcctttg
     2461 cccggttagg acatccgtct aaacacgaag tgctgatgat cggtgatagc tggacttcgg
     2521 acatccaggg tgcggtccag tatggcatcg acgcctgttg gtataacccg gggcgcaaac
     2581 cccgccccgc caattacgag attacccgcg agatcgcctc gttgcgtgag ttaaacgact
     2641 ggctgggata gccagggttg acacttgcca gcctcaaaag gtgtctcctc tatggtttga
     2701 gcgagggaat ggcaatacct catattcgca gggccgcgca cagtgcccgg aaactacatc
     2761 ggctcccggt tcacgtcctg ataatagatg caactggcgg gtttcttgcc catcgccaca
     2821 aaccattgcg gacaataagg cgccgcccag atcacatca
//
LOCUS       DATCGS010000138         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_458487, whole genome shotgun sequence.
ACCESSION   DATCGS010000138 DATCGS010000000
VERSION     DATCGS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458487"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VIL39_02760"
     CDS             <1..298
                     /locus_tag="VIL39_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480376.1"
                     /translation="FGLCWGSNAVLGFFLSRGQRWARICLGVEGVLFLTYYYFRYGFP
                     LPHFPAWVSLVIFRLGSLTVGQVPYVSRWVFIALALASVCALFWPRKKTASYAC"
     gene            771..2387
                     /locus_tag="VIL39_02765"
     CDS             771..2387
                     /locus_tag="VIL39_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5480377.1"
                     /translation="MRLRRTMLVAASLAWTGLAWGYAVGPPLSLDKLAAEADLIFKGT
                     AVSSQPVQDEWFKPCPGFVVTETEFSVISVIKGDTPGPRLRFRHYDETKQPEGRMFQP
                     QYYHFEAGRTYLVFAKRSETAGAFRQLQANHTGKEDQGVQLCSNDQPVGARTVKEAMW
                     DELTLMLKSADATNVTYAIRQLDQMSGRRDGFRDTQDFDRTNVLNVVHGLMSSSSAMV
                     AQAAIAAVGSHNPYLSDERTIFWLATVGSGVVPGMGKMDPKMKNAGGELYWKELLALA
                     DSEAARDTRVLAIQALGLVREPALKKPIARWLSDSDPAVRAAATLLLADFPGPERSKR
                     LAVLAADAAPKVRACVARAAGFGQQEEAADTLSKLLADREEKVRTVAAMSLLSFSPKN
                     KAIANVFRANLENENFKPLFLLALARENPADYLDALARAVEQKTEPKNFWGGQIPAFT
                     AWEILFRYLQGQSAETLRSGKLDRYLDAMEKVGNYSSSEPRDIYAFYLQRGLTERAKK
                     FRTAASKVASYDLDYYFKEVDANPGLYQRH"
     gene            complement(2500..3084)
                     /locus_tag="VIL39_02770"
     CDS             complement(2500..3084)
                     /locus_tag="VIL39_02770"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY5480378.1"
                     /translation="MIAAKPRPVPAAWKNRRWTFEELVAELPESNLPTELWDGELIMS
                     PAPSFLHQKIVARLYKALDAWVEQHHLGDTGLAPIDMVLTTRRATQPDVVFISNERLG
                     IIKERIMGAADLVAEVISPGSRRRDRIDKRDLYEQHGVREYWLIDPEAGTVEVLFLES
                     GTYQLVGRWHTGERARSRLLKGFEVPVGPLFGEG"
     gene            complement(3231..3359)
                     /locus_tag="VIL39_02775"
     CDS             complement(3231..3359)
                     /locus_tag="VIL39_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480379.1"
                     /translation="MVDHAVRKGVKFDKPALGVTPQADASPCNGCLSSRRCLQFIT"
     gene            complement(3613..3960)
                     /gene="mazF"
                     /locus_tag="VIL39_02780"
     CDS             complement(3613..3960)
                     /gene="mazF"
                     /locus_tag="VIL39_02780"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEY5480380.1"
                     /translation="MKGRGYVPERGDAIWFDFDPQAGREQAGRRPAIVLSPGAYNVKS
                     GLAIICPITSRVKGYPFETPLPDGLPISGVVLADHARSLDWNSRRAELICQLPAEVVQ
                     DVAAKIIALVGDD"
     gene            complement(3960..>4176)
                     /locus_tag="VIL39_02785"
     CDS             complement(3960..>4176)
                     /locus_tag="VIL39_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY5480381.1"
                     /translation="WGNSLALRIPQAVARQIHVSNGDSVELQVEADGLRIRSARRRYR
                     LAQLIGKITPANRHTETAWGKSAGKEL"
BASE COUNT          772 a         1293 c         1299 g          812 t
ORIGIN      
        1 tttcggcttg tgctggggct ccaacgccgt tctgggtttc tttctatcgc gggggcagcg
       61 ctgggcgaga atctgcctcg gcgtggaggg cgttctgttc ctaacctatt actacttcag
      121 atacggtttt cctctgcccc acttccctgc ctgggtgtcc ctggtaatct tccggctggg
      181 ctcgctcaca gtcgggcagg tcccctatgt atcccgctgg gtattcatcg ccctcgcgtt
      241 agccagcgtc tgcgccctgt tctggccgcg caagaagact gcttcctacg cgtgttgagg
      301 ggcagaacct ttgaagcctc ccctgcaaac aggtgattca agttcgcctt ccgcagggga
      361 cacttcctcc acttcggagc cttttgatac tatccgtgtc gcttagtggg acctcctctc
      421 gcgtgcgggt cgagaggagc aattcgcgcg agccaccgtc taatctttat gcaacgcgtc
      481 cgacgggcat tacttctcgc tctgggcacg gcgattgggc tgccgggttc ggtcgcccat
      541 tccgccgagc ggcccgctgt ggacgtctca actgttcaca aaacgggatg gcgatgctcc
      601 cggaaagacg tggcgctttc gcgtcaggga ggacggcaaa gtcgaagcga tgccctcttc
      661 ccacacgcct gctaatcccc gataatggca acagcatgac tgagctcctg caacaaccct
      721 ttggcctgag ggccccacct gcggccaccg ctgcgctggc aggcgttgcc atgagactgc
      781 ggcgaaccat gctcgtagcc gcctcgctgg catggacggg cctcgcctgg ggctacgcgg
      841 ttgggccacc gctgagcctg gacaaacttg ccgccgaggc ggatctcatc ttcaagggca
      901 cggcggtctc aagccaaccg gtgcaggatg agtggttcaa gccctgtccg gggttcgtgg
      961 tcacggagac agagttcagc gtcatctcag tcatcaaagg tgacacgccc gggccgaggc
     1021 ttcgattccg ccactacgat gaaaccaagc agcctgaggg ccgaatgttt cagccgcaat
     1081 actaccattt cgaggccgga aggacttacc ttgtgttcgc caaacggagc gaaaccgcgg
     1141 gggccttccg ccagctccaa gcaaaccaca ccgggaagga agaccagggc gtgcaattgt
     1201 gctcgaacga tcagccagtg ggggcacgga ccgttaagga ggcgatgtgg gacgagctga
     1261 cgctgatgct caagagcgcc gatgccacca acgttacata cgccatccgc cagctcgacc
     1321 agatgagcgg caggcgggac ggtttcagag acacgcagga tttcgaccgc acaaatgtgt
     1381 tgaatgttgt tcacggcttg atgtcgagtt ccagcgcgat ggtcgcgcaa gccgccatcg
     1441 cggcggtcgg ctcgcacaac ccctacctgt ccgacgagcg gaccatcttc tggctggcta
     1501 ccgtgggcag tggcgtggtg cctggcatgg gcaagatgga ccccaagatg aagaatgccg
     1561 ggggtgaact ttattggaag gagctcttgg ctttggcgga cagcgaggcc gccagggaca
     1621 ctcgcgttct ggccatccag gcgctcgggt tggtgcgcga accagcgcta aagaagccca
     1681 tcgcacgctg gctgagtgat tctgatccgg ccgttcgcgc cgcggccacg ctcctgctgg
     1741 cggacttccc cgggccggag cgttccaagc gtctggcggt gctggctgcc gatgctgcgc
     1801 ccaaggtgcg ggcatgcgtg gctcgcgcgg cgggctttgg tcagcaggag gaggcggctg
     1861 acaccctgtc caaactactg gcggaccggg aggagaaagt ccgcacggtc gcggcgatga
     1921 gtctgctgtc gttctcgccg aagaataagg ccatcgccaa tgtcttccgc gccaaccttg
     1981 agaatgagaa cttcaagccg ctattcctcc tcgcattggc gcgcgagaat cccgccgact
     2041 accttgatgc cctcgcgcgg gcggtggaac agaagaccga gccaaagaac ttctggggcg
     2101 gccagatacc tgctttcact gcctgggaga tcctgttccg ctatcttcag ggccagtcgg
     2161 ccgaaacgct gcgctccggc aagctggatc gctatctgga cgcgatggag aaggtgggca
     2221 actattcgtc gtccgagccg cgggacatct acgccttcta cctccagcgc ggcctgaccg
     2281 agcgcgcgaa gaagttccgc acagccgcga gcaaggtcgc atcctacgat ctcgattact
     2341 acttcaagga ggtggatgcg aatccgggac tctaccagcg gcattaggcg cgtcgatggg
     2401 gcagggtatg aatgatggct cgtccgctga gatgaggcga tggaggaaga ccgatgctcc
     2461 gctccgcgtt ttcggcacgg cggcctcaac cgccccagct cagccctcgc cgaacagcgg
     2521 tccgacgggc acctcgaagc ccttgagcag gcgcgagcgg gctcgctctc cggtatgcca
     2581 gcggccgact agttggtagg tgccggattc cagaaacagg acttcaacag tcccggcttc
     2641 agggtcaatg agccagtatt cccgaacgcc gtgctgttcg tagaggtcgc gcttgtcgat
     2701 ccggtcacgc cgccgggacc cgggggagat gacctcggcc accaagtccg cggcgcccat
     2761 gatgcgctcc ttgataatgc ccaggcgttc gttggaaatg aagaccacat cgggctgcgt
     2821 ggcgcggcga gtggtcagca ccatatcaat tggggcaagc cccgtgtctc ccaggtggtg
     2881 ctgctcgacc caggcatcca gtgctttgta caaccgagcg acaatcttct gatgaagaaa
     2941 tgaaggggca ggggacataa taagttctcc gtcccacagt tccgtgggca ggttgctctc
     3001 gggcaattcg gcgaccagtt cctcaaaggt ccaacggcga ttcttccagg cggcgggcac
     3061 gggtctgggc ttggctgcaa tcacaggaac aatcaacccc ggaatggggc tgccgtcaag
     3121 cttcggcttt cggcgtccaa caactcctca ctcttaacac ttccggccct ggctgataac
     3181 ggtgcaccaa gccgccctgg ctgtgcatcg aatatcctgg cggcggttca tcaggtgatg
     3241 aactgcaggc atcggcgcga cgaaaggcag ccgttgcaag ggctggcgtc ggcctgcggc
     3301 gtaacaccca aagccggctt gtcgaacttc acgcccttgc ggactgcgtg atccaccatg
     3361 tccgcaatat agcggcggcg ttcggcgagc ggcgaaggtg gcgaacgccc ccgcgcaaag
     3421 ctaccgcgag taccgctcgg gtaccacccc ggccggcatt gtggatatgg ccgttcccgg
     3481 cttgagaccc gtgcggccga atacgcctct gaaccctcgc atcagccagc tccgcgggca
     3541 agttaatggt tgatggttaa gagttgagag ttctaacgct cgtgctcgcc ctccccttga
     3601 ccagttcagt tttcaatcgt cgcctaccag cgcgatgatt ttggcggcaa cgtcctgaac
     3661 cacttcggcg ggaagctggc agatcaattc ggcgcggcgc gaattccaat ccaatgaccg
     3721 ggcgtggtcg gccagcacca cgccgctgat gggcaggccg tccggcaacg gtgtttcaaa
     3781 aggatagcct ttcacccggc tggttatcgg acagatgatc gccagtccgg acttcacatt
     3841 gtaagcccct gggctcaaca cgatggcggg tcggcgtccg gcctgctcgc gtccggcttg
     3901 cggatcaaaa tcgaaccaga tggcgtcgcc acgttccggc acataaccgc ggcccttcat
     3961 caaagctcct tgccggcgga ttttccccat gcagtttcag tgtggcggtt ggccggggtt
     4021 atcttgccga tgagctgggc gaggcggtag cggcggcggg cggaacgaat ccgcagtccg
     4081 tctgcctcca cctgcagttc cacgctgtct ccgttgctga cgtgaatctg ccgggccacg
     4141 gcctggggaa tgcgcaaggc caggctgttg ccccac
//
LOCUS       DATCGS010000139         4695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_259811, whole genome shotgun sequence.
ACCESSION   DATCGS010000139 DATCGS010000000
VERSION     DATCGS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259811"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4599)
                     /locus_tag="VIL39_02790"
     CDS             complement(<1..4599)
                     /locus_tag="VIL39_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY5480382.1"
                     /translation="MNLPLQAGPRDAQWQQVEEAIKKGLPKTAITNLEPIIQGALKDK
                     AYAEAVKAIGQKIALEGNIQGNKPEEKITRLEAEIAKAPKEMTPLMDTLLAEWYWQYF
                     QQNRWRFMQRTATAAQPGKDFTTWDLPRLFREIDRQFQKALAAEKILKATPISAWNDL
                     LRKGTMPDSYRPTLYDFIAQQALAFYTSGEQAAAKPEDAFELSADSPILDSAEKFLAW
                     QPAAGADTDSPVLKAVRLYQDLLRFHQSDPSPRFAFATADLERLTWGWNIAFGEDKNT
                     RYKTALEAFIRTYADFDISALALEREARVVQQEGDLVAARKLALRGVELYPQSPGGKL
                     CRNLVTEIEAKSASITTERVWNCFPGSNSGAGVPPANQEGRRDACPTITVRYRNVEAV
                     YFRAIPYDWELFLDKRHHRPENLSEQERREILARQPALEWSSKLPSTTDYKEKTLATP
                     APDSLKPGFYFIAASHDPKFGEGNNVISMTDVWVSGLALVTRMHDGRMEGFVLKADSG
                     EPVTGAEVAVWHLDNQGNRIADPTLTTDENGFFSIEPAQNAGYLFRARSGGQELATAS
                     DLWAYGYQNPQEPRPAAQTVFFTDRAIYRPGQAIQYKGICLWVDQTKDNYEVLKGEEL
                     TVVFKDVNGKEVARQKQRANDYGSLAGSFTAPRDRLMGQMSLQVEGRAQGVGYLRVEE
                     YKRPKFEVTLDAPKTAAKLNEQVSLTGHAMSYTGAAVDGSAVKYRIVREVRMPWWWGW
                     WRGGSPHSQSQEIAHGTARTGTDGAFKIEFSAKPDPKVPEKDEPTFVFQINADVTDSA
                     GETRSADRAIRVGYTALEAMISADDWQTDGKPVELKLETKTLDGEPQVAEGSVKIYEL
                     QAPEKAHRPPMSQGGPRSPSYASDESDMSNTSPDLSNPNNWPLGKVVAEKGFTTDTNG
                     AAKLAFKLAAGAYRAVLETQDRFGKKVTGKLPLEVLQPDATKLGIRIPQLLAAPDWEL
                     QPGQEFMALWGTGYETGRAFIEIEQQHQMVQRYWTKTGQTQQQIKVAVTEAMRGGFQV
                     HVTCVRENRAYFQSRKVNVPWKNKELDLKWEHFVSKLQPSQKETWTIVVQSPKSKVQS
                     AEKAVAEMVATLYDESLDAFAPLNWPHQFSVFREDYSVMQSQFANTPMGFQQVFGSWD
                     RPYESVEITYRSFPPDLTQNLWGYRFYARKAMNVTTLSGARSEAAAPMAMAAEMPAMA
                     SPPPGAAFQAGAAVDRLGVEALADKDKRESGGGGAGGANKQPAPPKPDLSKVAARKNL
                     NETAFFFPQLTSDSNGVVRMTFTMPEALTKWRFLGFAHDQSVRSGFLEDHAVTAKDLM
                     VQPNPPRFLREGDTVEFTVKVSNQTDKPLRGKVQLTFNQALNSQSADKLLGNTKPDQD
                     FDIPAKESRSCAWRIHVPNGCGFLTYKAVGAAANVSDGEEGYLPVLSSRILVTESLPL
                     PIRGPATKKFEFTKLLKSGGSKTLEHRSLVVEMVSNPAWYAVLALPYLMEYPHECSEQ
                     IFNRLYANALARTIANGDPKIRRIFDQWKNTPALESPLQ"
BASE COUNT          763 a         1443 c         1489 g         1000 t
ORIGIN      
        1 ctgcagcgga ctctctagcg cgggggtgtt cttccattgg tcgaagatgc gtcggatctt
       61 tgggtcgccg ttcgcgatgg tgcgcgccag ggcattggca taaagccggt tgaagatctg
      121 ctcgctgcac tcgtggggat attccatcag gtagggcaac gccagcacgg cataccacgc
      181 cgggttggat accatttcca ccacgagact ccggtgctcg agggtcttcg agccgcccga
      241 tttgagcagc ttggtgaact cgaacttctt cgtcgccggg ccgcgaatcg gcagcggcag
      301 cgactcggtg accaggatgc ggctggagag caccggcaga tagccttcct cgccgtccga
      361 cacgttggcc gccgcgccga cggccttata ggtcaggaaa ccgcagccat tcggcacgtg
      421 gatgcgccag gcgcaactgc gcgattcctt ggccgggatg tcgaagtcct gatccggctt
      481 cgtgttgccg agcagcttat ccgcggactg actattgagc gcctgattga aagtaagctg
      541 caccttgccg cgcaagggct tgtccgtttg gttggacacc ttgaccgtga actcgacggt
      601 gtcgccctcg cgcaggaagc gaggcgggtt gggctgcacc atgaggtcct tggcggtcac
      661 cgcatggtct tcgaggaaac cgctgcgcac cgattggtca tgcgcgaagc ccaggaaccg
      721 ccacttcgtc agcgcctcgg gcatggtaaa ggtcatccgc accacgccgt tggaatccga
      781 ggtcaactgc gggaagaaga acgccgtctc attcaggttc ttgcgggcgg cgaccttgct
      841 caagtcgggc ttcggcggcg cgggctgctt gttagcgcca cccgccccgc cgccgccgga
      901 ttccctctta tccttgtcgg ccagagcttc tacccctaac cggtccaccg ctgcccccgc
      961 ttggaacgcg gcccctggcg gcggcgaagc catagccggc atctcggcgg ccatcgccat
     1021 tggtgctgcg gcctcacttc tcgcaccgct gagagtcgtc acattcatgg cttttctggc
     1081 ataaaaccga tacccccaga gattctgggt caggtctggc gggaaggaac gataggtaat
     1141 ctcaacgctc tcgtacggcc ggtcccagct tccgaagacc tgctggaacc ccatgggtgt
     1201 attggcaaac tgcgactgca tgacggagta atcttcgcga aacacggaga actggtgcgg
     1261 ccagttcaat ggcgcgaagg catccagcga ctcgtcataa agcgtcgcta ccatttccgc
     1321 gaccgccttc tccgcacttt ggactttgga ctttggactt tggactacga tcgtccacgt
     1381 ttccttctgg ctgggttgga gcttggagac gaagtgctcc catttcaggt ccagctcctt
     1441 gttcttccat ggcacattga ccttgcgcga ttggaagtaa gcacggtttt cgcggacgca
     1501 ggtaacgtgc acctggaacc cgccgcgcat tgcctcggtc acggccacct tgatctgttg
     1561 ctgggtttgg ccggtcttgg tccaatagcg ctgaaccatt tggtgctgct gttcgatctc
     1621 gatgaacgcg cgccccgttt cgtaacccgt gccccatagc gccataaact cctggccggg
     1681 ctgtagctcc caatccggtg ccgccagtaa ttgcggaatc ctgatcccca gcttcgtagc
     1741 atcgggttgc agcacctcga gggggagttt gcccgtgacc ttcttgccaa accggtcctg
     1801 ggtctccaac accgcccgat atgctcctgc cgccagcttg aatgccagct tcgccgctcc
     1861 gttggtgtcg gtggtgaagc ccttctccgc gaccaccttg cccaacggcc agttgttcgg
     1921 gttggagagg tccggggaag tattggacat gtcagactcg tcggacgcgt aggacgggga
     1981 cctgggcccg ccttgagaca ttggcggccg gtgtgccttc tctggcgcct gcagttcata
     2041 aatcttcacg ctgccctcgg ctacctgtgg ctcgccgtcc agggtcttcg tctccagctt
     2101 cagctcgacc ggcttgccgt cggtctgcca atcatcggcg ctgatcatcg cttcgagcgc
     2161 ggtgtagccg acgcgaatgg cgcgatcagc ggagcgtgtc tcgcccgcgc tgtcggtcac
     2221 atcggcattg atctggaaaa caaaggtggg ttcgtccttc tccggcacct tcgggtccgg
     2281 cttggcgctg aactcgatct tgaatgcgcc gtcggtgccg gtcctggctg tgccgtgggc
     2341 gatttcctgg ctttggctat gaggcgagcc gccgcgccac caaccccacc accacggcat
     2401 tcgcacttcg cgcacgatgc ggtatttgac cgccgacccg tccaccgccg cgccggtgta
     2461 gctcatggcg tgcccggtga ggctcacctg ttcgttgagc ttcgccgcgg tcttcggcgc
     2521 gtcgagcgtc acctcgaact tgggccgttt gtattcctcg acccggaggt agccaacgcc
     2581 ctgcgcccgc ccttccacct ggagggacat ctggcccatg agccggtcgc gtggggcggt
     2641 gaagctgccc gcgagcgagc cgtagtcgtt ggcgcgctgc ttctggcggg caacttcctt
     2701 gccgttcacg tccttgaaca ccaccgtcaa ctcctcgccc ttgagcactt cgtagttgtc
     2761 tttggtctgg tccacccaga ggcagatgcc tttgtattgg atggcctggc cgggccgata
     2821 gatagcacgg tcggtgaaaa agacggtctg cgctgctggg cggggctcct gcgggttctg
     2881 atagccgtaa gcccagagat cgcttgcggt ggccaactcc tgaccgccag agcgcgcgcg
     2941 gaagaggtag ccagcattct gcgctggctc aatggagaag aacccgttct catccgtggt
     3001 cagcgtcgga tcggcgatgc ggtttccctg gttgtcgaga tgccagacgg ctacctcagc
     3061 gccggtgacg ggttcgcccg aatcggcctt caggacgaag ccttccatcc tgccgtcgtg
     3121 catccgcgtc acgagcgcca ggccgctcac ccatacgtcg gtcatggaaa tgacgttgtt
     3181 cccttcaccg aacttcgggt cgtggctggc ggcgatgaaa tagaagcccg gcttgaggct
     3241 gtcgggtgct ggggttgcca aggtcttttc cttgtagtcg gtagtggatg gcagcttcga
     3301 ggaccattcc agcgcgggct ggcgggccag gatttcccgg cgctcctgtt cgctcaggtt
     3361 ctctggccga tgatggcgct tgtccaggaa gagttcccag tcatacggga tagcgcggaa
     3421 atacaccgcc tccacgttgc ggtagcggac ggtgatagtg gggcaggcgt ctcgcctgcc
     3481 ttcttggttc gcaggcggga cgcctgcccc actattactg ccagggaagc aattccagac
     3541 ccgctcggtg gtgatgctgg ccgacttggc ttcaatctcg gtgaccaggt tgcggcagag
     3601 tttcccgccg ggcgattgcg ggtatagctc cacgccgcgg agggcgagct tgcgcgccgc
     3661 gacgaggtcg ccttcctgct ggacgacccg cgcctcgcgc tccagggcca gcgcggagat
     3721 gtcgaagtcg gcataggtgc ggatgaatgc ctccagagcc gtcttgtagc gggtgttctt
     3781 gtcttcaccg aaggcgatgt tccagcccca cgtcagccgc tccaggtctg cggtggcaaa
     3841 ggcaaaccgc ggggaggggt cgctctggtg gaagcggagc aagtcctgat agagacggac
     3901 ggctttgagc acgggcgagt cggtgtcggc gccggcagcc ggctgccagg ccaggaactt
     3961 ctctgcggag tcaaggatcg ggctatctgc ggacagttcg aaggcatcct ccggcttggc
     4021 cgccgcctgc tcgccggagg tgtagaaggc cagggcctgt tgggcgatga agtcgtagag
     4081 agtgggccgg taactgtccg gcatggtccc cttccgcaag aggtcattcc aggcgctgat
     4141 gggtgtggct ttgaggatct tctccgccgc gagcgccttc tggaactggc ggtcaatctc
     4201 ccggaagagc cggggcaggt cccaggtcgt gaagtctttg cccggctgcg cggcggtggc
     4261 cgtgcgctgc atgaaacgcc accggttctg ctggaaatac tgccaatacc attcggccag
     4321 cagcgtatcc atcagcggcg tcatttcctt cggcgccttc gcgatctcag cctccaggcg
     4381 cgtgatcttc tcttccggct tgttgccctg gatgttgcct tccagcgcga tcttctgtcc
     4441 gatggccttt accgcctcgg cataggcctt gtctttcagc gcgccctgga tgatcggttc
     4501 gaggttggtg atggcggtct tgggcaggcc cttcttgatg gcctcctcga cctgctgcca
     4561 ttgcgcgtca cgcggaccgg cctgaagggg gagattcata agcagcagag tggcaatgca
     4621 agcaatgaga atggaggatg gaagatggct gaataacgcg gtgggaccaa cgcaaaggcg
     4681 caaaggtgca aagac
//
LOCUS       DATCGS010000140         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_706043, whole genome shotgun sequence.
ACCESSION   DATCGS010000140 DATCGS010000000
VERSION     DATCGS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706043"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1934
                     /locus_tag="VIL39_02795"
     CDS             306..1934
                     /locus_tag="VIL39_02795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY5480383.1"
                     /translation="MAEPKTGELMEKIVSLCKRRGFIFQSSEIYGGLNGFWDYGPLGA
                     ELKRNIKECWWRAMTQLRDDVVGLDASIIMHPKIWEASGHTSTFSDLMVDCLLTKKRF
                     RADQVEPQSGTAYFYTGASDGTGVKESKDAFSVLIAPSKHADYARKVAKQFYQQRGIE
                     NPALLGERTEEVKDSTRYNPENGSLLTEPRPFNLMLKTYVGPVESEDNKAYLRPETAQ
                     AIFVQFKNTLDVSRQRVPFGVCQIGKAFRNEINPRNFTFRSREFEQMELEFFIRPDEV
                     VQKMTGAVASLSDPQPSTLNPQPNWGWELWHKYWVEQRLKWYESIGLGRDTLVEYWQK
                     KEELAHYARATVDILFKFPFGTQELEGIAARGNFDLTQHQKFSGKSMEYFDEEAKAKY
                     VPHVIEPSAGVDRLALALICNAYCEDQAPDEKGKMESRVVMKFHPRIAPIKVAVFPLL
                     KNKPALVEKAKAVRDLLRPHMSVFYDEAGAIGRRYRRQDEAGTPFGVTIDFDTLGEQG
                     PELQDTVTLRDRDTMKQERVKIGDLASMLLAKIR"
     gene            1934..2449
                     /locus_tag="VIL39_02800"
     CDS             1934..2449
                     /locus_tag="VIL39_02800"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEY5480384.1"
                     /translation="MGTESELVKKRAPTLYAIIAIKLLKGLLFVTLAIVAYTLSDNNL
                     PAEYQNLLHHLRLSPERKFWADLAVQIGQLTEANVLWAAAGTLVYSLFSLVEGVGLML
                     RISWAGWMAIGESAFFIPIELYELVHRTVPARPRPGHTVMVLAILGLNILIVWYLFQN
                     RHRLFRHHHHP"
     gene            2780..3136
                     /locus_tag="VIL39_02805"
     CDS             2780..3136
                     /locus_tag="VIL39_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480385.1"
                     /translation="MKTLFCFCLAASLFLIGCGGSSDKPAQPTNGAAAGSSPLSAPAD
                     YVGALGKAQQTAVKTVDTTSLNQAIQMFNVENGRNPKDLNELVEKKFIARVPAAPFGM
                     KLEYDATAGKVKAVKQ"
     gene            3133..>3627
                     /gene="guaA"
                     /locus_tag="VIL39_02810"
     CDS             3133..>3627
                     /gene="guaA"
                     /locus_tag="VIL39_02810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5480386.1"
                     /translation="MIEQIVILDFGSQYTQVIARRVRECNVYSVILRYDVPAAEIARL
                     KPKGIILSGGPSSVYAQNAPLPDRAIFNLGIPVLGICYGLQLLAQYLGGKVEPGQKRE
                     YGKGTLRLKDSRCPLFADLPKSMQVWNSHGDKLTKLPAGFKSVAVTENSACAAIENRA
                     GKMFG"
BASE COUNT          770 a         1034 c         1110 g          713 t
ORIGIN      
        1 ccactcttag ggatatttaa agcatgtttc atgatgattt agttatcgga taatacgctg
       61 aagcaggctg gtttgtctgc caaaaagtag caattctgcc catgaacctc aaaaagtgtt
      121 cgttgattat caagcactta cgcattttga ggttcatggg cgcaatgcgc gcaaagaggt
      181 ttcggggaag tctcattatg caccacatcg ggccgccggg ggttggccaa ggccatagtg
      241 agaactgctg ggcacgcttc ggggcatctt gacatgcggg cactctgtga gagactgccc
      301 ggcctatggc tgaaccaaaa accggtgagt tgatggaaaa gatcgtttcg ctctgcaaac
      361 gacggggatt catatttcag tcgtccgaaa tctacggcgg cctcaacggt ttttgggatt
      421 acggcccgct gggcgccgaa ctcaagcgca acatcaagga atgctggtgg cgcgcgatga
      481 cccaactgcg cgacgacgtg gtgggcctgg acgcctcgat catcatgcac ccgaagatct
      541 gggaggccag cggccacacc agcactttca gcgacctgat ggtggattgc ctgctgacga
      601 agaagcgctt ccgggcggac caggtcgagc cgcaaagcgg cacggcctac ttctataccg
      661 gcgccagcga tggcaccggc gtcaaggaat ccaaggatgc gttctcggtg ctgattgcgc
      721 cgagcaaaca cgctgattac gcgcgcaagg tggcgaagca attctatcag cagcggggga
      781 tcgagaatcc ggcgctgctc ggcgaacgca ccgaggaggt caaagacagc acccgctaca
      841 acccggaaaa cggcagcctg ctcaccgaac cgcgcccctt caacctgatg ctgaagacct
      901 acgtcggccc ggttgaaagc gaggataaca aagcctatct ccgcccggaa acggcgcagg
      961 ccatcttcgt gcagttcaag aacacgctgg acgtctcgcg ccagcgcgtg ccctttggcg
     1021 tttgccagat cggcaaagcg ttccgcaatg agatcaaccc gcgcaacttc accttccgct
     1081 cgcgcgagtt cgagcagatg gagttggagt tcttcatccg cccggatgag gtggtgcaga
     1141 agatgacggg cgcggtcgcg agcctctccg accctcaacc ttcaaccctc aaccctcaac
     1201 ccaactgggg ctgggagctc tggcacaagt attgggttga gcagcgcctc aagtggtacg
     1261 agagcatcgg cctgggccgc gatacgctgg tcgaatattg gcagaagaaa gaggagctgg
     1321 cgcactacgc ccgggccacc gtggatatcc tattcaagtt cccattcggc acgcaggagc
     1381 tggagggcat cgcggcgcgc ggcaactttg acctgacgca gcaccagaag ttcagcggca
     1441 agtccatgga gtacttcgac gaggaggcca aggccaaata cgtgccgcac gtcatcgagc
     1501 cgagcgccgg ggtggatcgg ctggcactgg ccctgatttg caatgcctac tgcgaggacc
     1561 aggcgccaga tgagaagggc aagatggaat cgcgagtggt gatgaagttc catccgcgca
     1621 tcgcgccgat caaggtggcg gtgtttccgc tgctcaagaa caagccggcg ctggtggaaa
     1681 aggccaaggc cgtgcgtgac ctcttgcgtc cgcacatgtc tgtgttttac gacgaagcgg
     1741 gcgcgattgg ccggcgttac cggcggcagg acgaggcggg cacgccgttc ggtgtgacga
     1801 ttgacttcga tacgctgggc gagcagggcc ccgagttgca ggacaccgtc acgttgcgcg
     1861 accgcgatac catgaagcag gaacgagtga agattggcga tctggcatct atgttactgg
     1921 cgaaaatccg gtaatgggca cggagagcga actggtcaaa aagcgagccc cgacgcttta
     1981 tgcgatcatc gccataaagc tgctgaaggg gctgctgttc gtgacgctgg cgattgtagc
     2041 ctacacgctc tcagacaaca acctgccggc ggagtatcag aatctgctgc accacctgcg
     2101 gcttagtccg gagcggaagt tctgggccga cctcgcggtg cagatcggcc agcttaccga
     2161 ggcgaacgtc ctatgggctg cggcgggaac actggtctat agcctgttct cgctcgtcga
     2221 gggggtgggc ttgatgttgc gaataagctg ggccggctgg atggccatcg gggaatcggc
     2281 cttcttcatc cccatcgaac tttacgagtt agtgcaccgg accgttccgg cgcggcctcg
     2341 tccaggtcac acagtcatgg tgctggcgat cctcgggctc aatatcctga tcgtctggta
     2401 cctgttccag aaccgccacc gcctgttccg gcatcaccat catccttagc ggttgggggt
     2461 ggctgacatt tgcccaaaag attgtacttg tcctttttgc gcttttgata aaggctgagc
     2521 ccactatgaa acggatattc acacttatcg tattgttggg gattgtaatg ggcgctgtgc
     2581 tgacgggctg tagtccgagt tcttcgactc agacaactac cgacacgaac gccgctccta
     2641 tggcccccgc aagcaccaac aagtaggaca ttttccccga atcagttacg ggagtgtcac
     2701 tttcacgccg ggcaatggtc ttgcccggcc tttttattgt ttcgcttgga attggctggc
     2761 gaggaactag actgaactca tgaaaacact gttctgcttc tgtttggccg ccagcctgtt
     2821 tctcatcggc tgcggtggaa gttccgacaa gcccgcccag ccgaccaacg gcgctgccgc
     2881 cggcagcagc ccgttgagcg cgccggcgga ttacgtgggc gcgctgggca aagcccagca
     2941 gacggcggtc aagacggttg ataccacttc gctgaaccag gccatccaga tgttcaacgt
     3001 ggagaacggg cgcaatccca aagacctgaa cgagctggtg gagaagaagt tcatcgcgag
     3061 ggttcccgcg gcgccgttcg ggatgaagct ggagtacgat gccacggccg ggaaggtaaa
     3121 ggcggttaag cagtgattga gcagatcgtc atcctggact ttggctcgca atacacgcag
     3181 gtcattgcgc gccgtgttcg cgagtgcaac gtctattcag tcatcctgcg ctacgacgtg
     3241 ccggcagcgg aaatcgcccg gctgaagccc aagggcatca tcctttcggg cggcccgtcg
     3301 agcgtctatg cccagaacgc gccgctgccc gaccgcgcca tcttcaatct cggcatcccg
     3361 gtgctgggta tttgttacgg cctccagctc ctagcccagt atctcggggg gaaggttgaa
     3421 cccggccaga agcgcgaata tggcaagggc acgctccgcc tcaaggacag ccgctgccct
     3481 ctgttcgctg acctgccgaa gagcatgcag gtatggaatt cgcacgggga caagctcacg
     3541 aagctgccgg cgggcttcaa atcggtggcc gtcacggaga actccgcttg cgcggccatt
     3601 gagaatcgcg ccggaaagat gttcggt
//
LOCUS       DATCGS010000141         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_112294, whole genome shotgun sequence.
ACCESSION   DATCGS010000141 DATCGS010000000
VERSION     DATCGS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112294"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VIL39_02815"
     CDS             <1..923
                     /locus_tag="VIL39_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480387.1"
                     /translation="LILRTYTDGAAPESSKWASFSTPPDAGGGLLPPLTVPGTIASLS
                     LYRDLHAFYAAKNALFPERTSGLIFFENMMGIFFSGIDLTEGVLSEVRPDVRVVVAKP
                     QYDPAVGTPATQVPAFAAVLRLKDPQQFGETVEEAWQKAFGLINFTRGQQALPGLSLD
                     KDSHNGVKYTLSYYRPPASKSSGPIDARYNYRPSLARPGDYVILSSTDALARDLIDVL
                     KQASPAPAKMPQAENGLMEIEGPALCAILEANRENMVRKSMVDKGNSREKAESDTQIL
                     LAALGHLDHATLTLRRDAGRPQAQLEIRFK"
     gene            920..2254
                     /locus_tag="VIL39_02820"
     CDS             920..2254
                     /locus_tag="VIL39_02820"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5480388.1"
                     /translation="MNPDPHDPAWAWATFQPDAARPWDLRLAAHLYRRAAFGGTWPEL
                     QRALSEGPQATIDKLVRPTPEATAFNRLYDDYENAAAASAGYGSQAWWLRRMIESPQP
                     LQEQVTLFWHNHFGISNVHVKSNASLCRHTHVLRQRGLSNFEELLDAVLAQPAVWICL
                     GARDQGKRSLSDRSVRIVMDQYTLGPGHCTDSDVREAARALTGWIITQGELRFVPAEY
                     DAAPKKILGQEGDFDRKALVRILAQHPATARWLARQLVRWFVSDTANPSDALLAPLAS
                     QLTGDGNIAKGVEMLLRSNLFFSTEAYRQKVRSPVELALGIIRPLEGRTGTLHLAADL
                     AGLGQDLYEPPTIQGWAGGLAWINSLTLLGRAKLAEALLAESGAYEGKLDPAAVTSRH
                     GHPAKDGARQFLLDLYLQGDLAPASLDALAAGSADARHVGARLASLPEFQLA"
     gene            2266..>2687
                     /locus_tag="VIL39_02825"
     CDS             2266..>2687
                     /locus_tag="VIL39_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480389.1"
                     /translation="MPISRREFFKETAGATSLLAFASSVPAFLARSAAALEAGQPARK
                     SALVVIQLSGGNDGLNTVVPYQDDVYHRSRPTLRIGPDQVLKLDSNLGLHPAMPAFRR
                     LYQEGRLCIIQGVGYPHSSRDHDAAMEDWHTGVLQERVP"
BASE COUNT          506 a          862 c          831 g          488 t
ORIGIN      
        1 agctcatctt gaggacctac acggatggcg cggctccgga atcgtccaag tgggccagtt
       61 tcagcacacc gccagacgcc ggtgggggtt tgctcccgcc actgacggtt ccgggcacga
      121 ttgccagcct gagcctctac cgcgacctgc acgcattcta cgcggccaag aacgccctgt
      181 tcccggaacg cacctcgggc ctgatcttct ttgagaatat gatggggatc ttctttagtg
      241 gcattgacct gaccgagggg gtgctgagcg aagtgcgccc cgacgttcga gtggtggtcg
      301 ccaaaccaca atacgatccg gcggtcggca cgcctgccac ccaggtgccg gcttttgcgg
      361 cggtgctgcg gctcaaagac ccgcagcaat tcggcgagac ggttgaagaa gcctggcaga
      421 aggccttcgg actcatcaac ttcacccgcg gccagcaggc gttgccgggc ctgagcctcg
      481 acaaggatag ccataacgga gtgaaataca ccctctccta ttaccggccg cccgccagca
      541 agtcctccgg gcccatcgat gcgcgctaca actaccggcc ttcgctcgcc cgcccgggag
      601 actatgtgat tctcagttcg accgacgcgc tggcccggga tttgattgat gtgctcaaac
      661 aggcgtctcc cgctccggcc aaaatgcccc aagcagagaa cggcctcatg gaaatcgaag
      721 gacccgctct ctgcgcgatc ctcgaagcca accgcgagaa catggtgcgc aagagcatgg
      781 tggataaagg taactctcgc gagaaagcgg agagcgacac ccagatcctg ctcgcggcgc
      841 ttgggcactt ggatcacgcc accctcaccc tgcgccggga tgcgggccgg ccccaggccc
      901 agttggaaat ccgcttcaag tgaaccccga cccgcacgat cctgcctggg cttgggcgac
      961 gttccagccg gatgccgcca ggccctggga cttgcggctc gccgcccatc tctatcgtcg
     1021 cgccgcgttc ggcgggacgt ggccagaact ccagcgcgcg ttgtcagagg gcccgcaagc
     1081 caccattgac aaactggtgc ggccgacgcc tgaagcaacc gctttcaatc gtctctacga
     1141 tgattatgag aacgccgccg cagcctccgc gggctatggt tcgcaggcgt ggtggctgcg
     1201 acggatgatt gagtcgccgc aacccctaca ggagcaggtg accctgttct ggcacaacca
     1261 ctttggcatc agcaacgtcc acgtcaaaag caacgcttca ctctgccgcc atacccacgt
     1321 cctccgtcag cgtggtttga gcaacttcga agagctgttg gacgccgttt tagcccagcc
     1381 cgccgtctgg atctgcctcg gggctcgtga ccaaggcaag aggagtttgt cggatcgctc
     1441 cgtccggatc gtgatggacc aatacacgct cggccccggg cactgcactg actccgacgt
     1501 tcgcgaggcg gcgcgcgcgc tcaccggttg gattatcacc cagggggaac ttcgcttcgt
     1561 gccggctgag tatgacgccg ccccaaagaa gatcctgggc caggaaggtg attttgatcg
     1621 caaggcgctg gtgcgtatcc tggcgcaaca cccggctacg gcccgctggc tggcgcggca
     1681 gcttgttcgt tggttcgtct cggacacagc caatccttcc gatgcgctgc tggctccact
     1741 tgccagccag ctcacaggtg atggcaacat cgccaagggg gtggagatgc tgctgcgctc
     1801 gaatctgttc ttttcgacgg aggcgtatcg gcaaaaggtc aggagcccgg tcgagcttgc
     1861 gctggggatt atccgtccct tggaagggag aaccggaacc ctgcatttgg cggccgacct
     1921 cgccgggctg ggccaggacc tgtatgagcc cccgacgatc caaggctggg cgggaggcct
     1981 cgcctggatc aactcgctca cgttattggg gcgcgcgaaa ctggcggagg cgttgcttgc
     2041 cgaatcgggt gcttacgaag gaaagctcga tccggcggca gttacaagcc ggcatggcca
     2101 cccggcaaag gacggggcca gacagttcct gctcgatttg tatttgcagg gcgacctggc
     2161 acccgcgagc cttgacgcat tagccgccgg ctctgccgat gcgcgccacg tcggcgcccg
     2221 cctcgcttca ctgcccgagt ttcaactggc ttgacgaaag gaccaatgcc tatctcccgg
     2281 agagaattct tcaaagagac cgcgggcgcg acgagtctgc tggcattcgc gagcagcgtt
     2341 ccggcattcc tggcgcgatc cgccgcggca ttggaggctg gccagccagc gcgcaaatcc
     2401 gccctcgtcg tcatacagct ttcggggggg aatgatgggc tgaacacggt ggtgccgtat
     2461 caagacgacg tctatcaccg gagccggcca acgctgcgca tcggcccgga ccaagtgctc
     2521 aagctggatt caaacctcgg attgcaccca gccatgccgg catttcggag gctctatcag
     2581 gaaggcagat tgtgcatcat acagggcgtg ggctatcccc attcgagccg ggaccatgat
     2641 gccgccatgg aggattggca cacgggggtg ctccaagagc gggtgcc
//
LOCUS       DATCGS010000142         4646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_260194, whole genome shotgun sequence.
ACCESSION   DATCGS010000142 DATCGS010000000
VERSION     DATCGS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260194"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..979)
                     /locus_tag="VIL39_02830"
     CDS             complement(155..979)
                     /locus_tag="VIL39_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480390.1"
                     /translation="MSGKCPSRCGSVEPQRGFTLIELLVVIAIIAILAALLLPALSRA
                     KSQAWTIACLNNLKQLEVCWHAYTLDNNDLLVPNNSVMGITSTGSGSSIAAGSSWCLA
                     DPTDVNVQNGLLFQYNSSLGIYHCPADHSTLTDPSGTTPGTRRARSYNLSQSVNGYPE
                     YDSFVFTNIPCSKKLTQVKNPNVANCLVFIDENEYTLLDSQFGMPTDCYNGTQTWWDM
                     PANRHSQGANLGFADGHAERWKWRVPKAYTTWIQGVPPEELPDWLRLKASIKQTMD"
     gene            1349..1675
                     /locus_tag="VIL39_02835"
     CDS             1349..1675
                     /locus_tag="VIL39_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5480391.1"
                     /translation="MKKTASLVIALLAIPTLSVCAADAKASYEKDCAKCHGADGKGET
                     KMGKKQGAKDYTDAKVQAELKDEAAFKAIKEGYKKDSKEVMKPSEGLSDDEIKGLVAY
                     MRKFKK"
     gene            1854..3335
                     /locus_tag="VIL39_02840"
     CDS             1854..3335
                     /locus_tag="VIL39_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY5480392.1"
                     /translation="MNSEPSPTPAPAKRSSVFRNWLSLTGLVVVVGSLFSFLLLLLLD
                     ALAHFANPYVGILTYLVAPGFLVIGLILAMLGAFLRHRQILKTSGPLPPLRIDLTRPR
                     DRRMLGFFLAGSVLFLLISALGSYQTYHFTESVQFCGQACHGVMKPEYVTYLHSPHAR
                     VACAECHIGKGANWYVRSKLSGTYQVYATLADKYPRPIPTPVKNLRPAQETCEECHWP
                     QKFVGNLEHTFYSFLADETNTPFTVRMLLNVGGGDATHGPVGGIHWHMNVGNKIEYIA
                     SDEARQKIPFIRVTELSQGVVTEFRSPRFTNAVDEASLRPMDCMDCHNRPAHRYQTPN
                     SAVSLAMALGKIDRGLPFVKSNAIYVLTLPYTNSTQALQSIATSLSERYPNDPRIRPA
                     IGAVQQIYTNNFFPEMKASWKVYPDNIGHKDWPGCFRCHDGLHKTADGKRSIKANDCN
                     ACHTILAQGSGAELDQLTPKGQKFKHPGDEVEGGCNDCHTGGL"
     gene            3361..>4646
                     /locus_tag="VIL39_02845"
     CDS             3361..>4646
                     /locus_tag="VIL39_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480393.1"
                     /translation="MRITLVLLAWCGLGLLPALAADKSPNSACLECHSDKTLTKTNAA
                     GKEVSLFIDEAKLKASIHPTNTCTDCHADITSKHPDDNVPAQPPSCAKCHEEPVKQYA
                     TSMHGVSHQIGASGAASCWDCHGTHDMLSHKDPKSPVFKLNLPGTCAKCHSNPGLTQE
                     YQMKYPQAASQYMDSIHGRALLKMGLIVAPSCNDCHGVHDIKRAVDRESPINHANVAK
                     TCGKCHVGIEETYNKSVHGQLLAKGDKRGPVCSDCHTAHEVETPKNGHFKMASDLRCG
                     KCHADRLEHYRDTYHGKAMALGKPNVASDVAACYDCHGHHDVLPPSNPASHLSKTNIL
                     ATCQQCHPGATIGFTEFKPHANPLDAKHYPLLHVVFLAMTGLLLGTFAFFGLHTLLWL
                     VRAIWLYLHDSKKFREAKIKTQVDDEWFTRFVPFER"
BASE COUNT         1007 a         1437 c         1332 g          870 t
ORIGIN      
        1 ggaactggga ttggctcaat cgcatggcct atattccgac cgacatcatg ccataatccg
       61 ccgcccgctg ggccgggggc aaagggatta acagcgaagg cgggagggcg cgaagatgag
      121 gtacagtctc aagttgcggc ccgccgcgtg ggagtcaatc catcgtttgc ttgatggaag
      181 ctttgaggcg gagccagtcg ggcaattcct ccgggggcac gccttgtatc caggtggtat
      241 aggctttcgg cacccgccac ttccagcgct cggcgtgacc atccgcaaag cccagattgg
      301 ccccctggct gtggcggttg gcgggcatgt cccaccaggt ctgggtgccg ttgtaacagt
      361 cggtgggcat gccgaactgt gaatccagga gggtgtattc gttttcgtca atgaagacca
      421 ggcagttggc gacgttggga ttcttgactt gagtaagttt cttagagcac gggatgttgg
      481 tgaagacgaa cgaatcgtat tccgggtaac cgttgacgga ttggctcagg ttgtaactgc
      541 gggcgcgccg cgtcccgggc gtggttccac tggggtcggt aagcgtggaa tggtcggccg
      601 ggcagtggta gatgccaagc gagctgttgt actggaacaa cagcccgttt tggacgttga
      661 cgtcggttgg atcggccagg caccaggagg agcctgcagc aatggagctg ccactgcctg
      721 tcgaggtgat acccattacg gagttgttcg gcaccagcag gtcattgttg tcgagggtgt
      781 acgcgtgcca gcagacctcg agctgtttca aattattgag gcaggcgatg gtccaagcct
      841 ggctcttggc ccgggaaagg gctggcaaga gcaacgcggc gaggatggca atgatggcga
      901 taacgaccag cagttcaatg agggtgaatc cgcgctgcgg ttcgacagaa ccacaacgcg
      961 agggacattt accgctcaat tcaaatggcc tgatttgccg catagcgtct ttcgcctaat
     1021 tcctatggat tcatgttaaa ccatactcgt tcgggccgga ttctcaagcc agcatgaatg
     1081 caagaccggc gctttaacgg cccaccgtga attaacgctg cttcttgtga tccgaagaag
     1141 actttgattg ccagcaacta acttgtgcgg cagaataggc cgcacgaata agacatctga
     1201 gagaaaagag ccaaacaagg cgcgggaacc atttgaatgg atgatttttc gggccgggca
     1261 gccccggcgg cgaactcgct ttcccgccca cagcggctct ggtccgaaag agaacaacgt
     1321 attgaactaa caagagacaa aaagaagtat gaagaagaca gcatcactgg tgatcgccct
     1381 gttggcgatt ccgaccttgt ccgtttgtgc cgccgacgcc aaggccagct atgaaaagga
     1441 ttgcgccaag tgccacgggg ccgacggcaa aggggagacc aagatgggca agaaacaggg
     1501 agccaaggac tacactgatg ccaaggtgca ggccgaactc aaggacgagg cggcgttcaa
     1561 ggcaatcaag gaaggctata agaaggatag caaggaggtc atgaagccgt ctgaaggctt
     1621 gtccgacgac gagatcaagg gcctcgtcgc ctacatgcgg aagttcaaga agtaaccgcg
     1681 agtcccggcg acgcgcgggc gccgtcggga tgcaaccgcg tttgcacatt gaccccgcgc
     1741 cctgatctcc tccccgcccg gcggggatgt cattggggcg cggggcggca ttgaaataaa
     1801 ctcggtgccg cccgcgtttc cagttcctag cactgaccca gcactagcca gccatgaatt
     1861 ccgagcccag ccccacgccg gcgcccgcta aacgctcctc cgtattccgg aactggctca
     1921 gtctcactgg cctggtagtg gtagtgggga gtttattctc ctttctcctg ctgctgttgc
     1981 tggatgcgct ggcccatttt gccaacccct atgtcgggat cctgacgtac ctggtggcgc
     2041 cgggcttcct ggtgataggc ctgatcctgg ccatgctggg agctttcctg cggcatcgtc
     2101 agatactcaa aacatccggc ccgcttcccc cgctacggat tgacctcacc cggccccgcg
     2161 atcggcggat gttgggtttc ttcctcgcgg gcagcgtgct gttcctgctg atttccgcgc
     2221 tcggttccta ccagacttac catttcactg agtcggtaca gttttgcggc caggcctgcc
     2281 acggggtcat gaagcccgag tacgtcactt acctgcactc accccatgcg cgggtcgcct
     2341 gcgccgaatg ccatatcggc aaaggcgcta attggtatgt ccgttccaag ttgtccggaa
     2401 cttaccaggt ctatgccacc ctggcggaca aatacccccg gccgatcccc acgccggtca
     2461 agaacctccg ccccgcccag gaaacctgcg aggaatgcca ctggccgcag aaattcgtcg
     2521 gcaacctcga acataccttc tactccttcc tcgccgacga gaccaacacg ccattcactg
     2581 tccgcatgct cctcaacgtc ggcggcggcg atgccaccca cggtccggtg ggaggcatcc
     2641 actggcacat gaacgtcggc aacaagatcg aatacatcgc cagcgatgag gcgcgccaga
     2701 agattccttt tatccgcgtg accgagctgt cgcaaggtgt cgtcaccgag ttccgctcgc
     2761 ccagattcac gaatgccgtg gatgaggctt ccctgcgccc gatggactgc atggattgcc
     2821 ataaccgccc ggcgcaccgc taccagaccc ccaacagcgc ggtcagcctg gccatggccc
     2881 tcggcaagat cgaccgcgga ttgccctttg ttaagtccaa cgcgatttat gtgctgacgc
     2941 tcccttacac caacagcacg caggcgctcc agagcatcgc gacaagcctg tccgagcgct
     3001 atccaaacga cccgcgcatc cgaccggcga ttggcgccgt gcagcaaatc tacacgaaca
     3061 atttcttccc ggagatgaag gctagttgga aggtttaccc ggacaacatt ggccacaaag
     3121 actggcccgg ctgcttccgg tgccacgacg gcctccacaa gacggcagat ggcaaacggt
     3181 ccatcaaggc caatgattgc aatgcctgcc acaccatcct ggcgcagggc agcggcgccg
     3241 aactggacca actcacgccc aagggccaga aattcaaaca cccgggcgac gaagtcgaag
     3301 gaggttgcaa tgattgccac accggcgggc tgtaaatccc ggcctaacca actgaccctc
     3361 atgcgcatca ccctggttct gctggcctgg tgcgggctcg gcttgctacc agctttggct
     3421 gcggacaaga gccccaacag cgcctgcctg gaatgtcact cggacaagac gctcaccaag
     3481 accaacgcgg ccggcaaaga agtctccctg ttcattgatg aagccaagct gaaggcttcg
     3541 atccatccga ctaacacttg tacggattgc cacgcggaca tcacttccaa gcacccggac
     3601 gacaacgtgc cggcgcagcc gcccagttgc gccaagtgcc acgaggagcc cgtcaagcaa
     3661 tacgcgacga gcatgcatgg ggtgagtcac caaattggcg cttccggtgc ggccagttgc
     3721 tgggattgcc acggcacgca cgacatgctc tcccacaaag atcccaaatc accggtcttc
     3781 aagctgaacc tgcccggcac ttgcgccaag tgccatagca accctggcct gacgcaggaa
     3841 taccaaatga agtatcccca ggcggcgtcg cagtacatgg acagcatcca cgggcgcgcc
     3901 ctgctcaaga tgggcctcat cgtcgccccc tcctgcaatg actgccacgg tgtccacgac
     3961 atcaaacggg ctgtggaccg cgaatcaccg atcaaccacg ccaacgtcgc caagacctgc
     4021 ggcaagtgcc atgtcggcat cgaggagacc tataacaaga gtgtccacgg ccagctcctc
     4081 gccaagggcg acaagcgggg accggtctgc tccgattgcc acaccgccca cgaagtcgag
     4141 accccgaaga acggccactt caagatggcc agcgacctgc gctgcggaaa gtgccacgcg
     4201 gaccggctgg agcattatcg cgatacttac cacggcaaag ccatggcgct tggcaaacca
     4261 aacgtcgcct ccgacgtcgc cgcctgctac gactgccacg gtcatcacga cgtgctgccg
     4321 ccgtcgaacc cggcgtcgca cctgtcgaaa accaacatcc tcgccacctg ccagcaatgt
     4381 catccgggcg ccaccatcgg ttttaccgaa ttcaagccgc acgccaatcc gctggacgcg
     4441 aaacattatc cgctgctcca cgttgttttc ctggccatga ccggcctgct gctgggcacg
     4501 ttcgccttct tcggactgca caccctcttg tggctggtgc gcgccatctg gctgtatttg
     4561 cacgactcga agaaattccg cgaggccaag atcaagaccc aggtggacga cgaatggttc
     4621 acgcggttcg tgccgttcga gcggtt
//
LOCUS       DATCGS010000143         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_656719, whole genome shotgun sequence.
ACCESSION   DATCGS010000143 DATCGS010000000
VERSION     DATCGS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656719"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..2043)
                     /locus_tag="VIL39_02850"
     CDS             complement(268..2043)
                     /locus_tag="VIL39_02850"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY5480394.1"
                     /translation="MTMDTIGKGNTSNKAVLAWVDEMAKLCKPDHVYWCNGSKAERKA
                     LIAEAVEQGILIKLNQKKLPGCYYHRSNPNDVARVEQSTFICTENQEEAGPTNNWMAP
                     KEMYQKLRGYAAGSMRGRTMYVIPYLMGPPGSPLSKVGVELTDSIYVVLSMGTMTRMG
                     EVAFRQLGASDDFNRGLHCMLDVNPERRFIAHFPKDNAIISVGSNYGGNVLLGKKCLA
                     LRIGSYLGRQEGWFAEHMLILGVESPTGEKTYVAAAFPSACGKTNFAMMMPPPHLKGW
                     KIWTIGDDIAWMRPGPDGRLYAINPEAGYFGVVPGTNSKSNPAAVKMISHDTIFTNVA
                     LTPDLDVWWEGKDGPPPKECLDWKGNPWTPDSKEKAAHPNSRFTAPMTNNPLLAPEAN
                     DPNGVPISAIIFGGRRSDTMPLIYQAFNWVHGVYIGATMGSEMTAAAVGGAGQVRRDP
                     MAMLPFCGYHMGDYFRHWINMHKTIKQPPRIFHVNWFRKNAEGQFLWPGYGENMRVLK
                     WIVDRCHARADAQETALGWVPSPTSLDLTGLTNFTPERLEEVQAIREDEWRHELILQD
                     ELFMKLHARLPKELIFQRELLLARL"
BASE COUNT          373 a          653 c          647 g          452 t
ORIGIN      
        1 gaaggcgatg tcgctcgcca gcaactaatt cgccgctcat cgttatttgt ccctgcgcgc
       61 ttcgtgcgtg cggggacttt ttgtacacag cagtcctcaa gctgggctgt aacacccgtt
      121 cgcttgtgct ggctgaggta aggcgagtct ggcctcagtg cgtcggaagt gcgtcggaga
      181 ggccgcttca aaccactgga cggcgcgctg aggacaaacg cgccctaccc gcaggcgcgg
      241 ggacgcctgc gccaaggagg ggctgcgtca cagtcgcgcc aggagcagtt cccgctggaa
      301 aatcaactct ttggggaggc gggcgtgcag cttcataaac agctcgtcct gcaagatgag
      361 ttcgtggcgc cactcgtctt ctcgaatagc ttggacctcc tcgagccgtt cgggcgtgaa
      421 attggtcagg ccggtcaggt cgagactggt ggggctgggc acccagccga gcgcagtttc
      481 ctgcgcgtcc gcgcgggcat ggcagcggtc cacgatccat ttgagcacgc gcatgttctc
      541 gccgtagccg ggccagagga attgcccctc cgcgttcttg cggaaccagt tcacatggaa
      601 gatccgcggc ggctgtttaa tggtcttatg catgttgatc cagtgccgga aatagtcacc
      661 catgtggtag ccgcagaacg gcagcatggc catcgggtcg cggcgcacct ggcccgctcc
      721 gcccaccgcg gcggcagtca tctccgagcc catggttgcg ccaatgtaaa cgccgtggac
      781 ccagttgaag gcctggtaga tcagcggcat cgtatcgctg cgccgcccgc cgaagatgat
      841 ggcgctgatc ggcacgccgt tgggatcgtt ggcctccggg gccagcagcg ggttgttggt
      901 catcggcgcc gtgaaacggc tgttcgggtg cgccgccttt tctttcgagt ccggcgtcca
      961 cgggttgccc ttccaatcga ggcattcctt gggcggcgga ccatctttgc cttcccacca
     1021 gacatcgaga tcgggcgtga gcgcgacgtt ggtgaagata gtgtcgtgtg agatcatttt
     1081 caccgccgcc gggttcgact tcgagttagt ccctggcaca acaccgaaat accccgcttc
     1141 gggattaatg gcatagaggc ggccatccgg ccctggcctc atccacgcga tgtcatcgcc
     1201 aatcgtccaa atcttccatc ccttgagatg cggcggcggc atcatcatgg cgaagttggt
     1261 cttgccgcag gcgctgggga aggccgcagc cacgtaggtc ttctcgcccg tcggcgactc
     1321 tacgcccagg atcagcatgt gctcggcgaa ccatccctct tgtcgtccga ggtaggagcc
     1381 gatgcgcagg gcgagacact tcttgcccag caacacgttg ccgccgtagt tcgagcccac
     1441 cgagataatc gcgttgtcct tggggaaatg cgcgatgaat cgccgctccg ggttcacatc
     1501 caacatgcag tggaggcccc ggttgaaatc atcactggcg ccgagttgcc ggaacgccac
     1561 ctcccccatg cgggtcatgg tccccatgct caacaccaca taaatggagt cggtcaattc
     1621 gacgcccact ttggagagcg gcgagccggg cgggcccatg agatagggaa tgacatacat
     1681 cgtgcgccca cgcatcgagc cggcggcata cccgcgcagc ttctggtaca tctccttcgg
     1741 tgccatccag ttgttggtgg ggccagcttc ttcctggttc tcggtgcaga tgaaggtgga
     1801 ctgctcgacg cgcgcgacgt cgttcgggtt cgagcggtgg taatagcagc ccggcagctt
     1861 cttttggttg agcttgatca gaatcccttg ctcaacggcc tccgcgatga gcgccttccg
     1921 ctccgccttc gagccgttgc accaataaac gtggtccggc ttgcagagtt tggccatctc
     1981 gtccacccag gccagcacgg ccttattgct cgtgttgcct ttcccgattg tgtccattgt
     2041 catagttaat atcctagccg gacaggctgt taccgtactc atgagcaaaa ggcaagaaac
     2101 cttctttact ctttctcctg gcggg
//
LOCUS       DATCGS010000144         4485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_62913, whole genome shotgun sequence.
ACCESSION   DATCGS010000144 DATCGS010000000
VERSION     DATCGS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62913"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VIL39_02855"
     CDS             complement(<1..376)
                     /locus_tag="VIL39_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480395.1"
                     /translation="MRKLLQPILAVALGLALGLGVTWIAGENPWHVFIILGKGAFGSS
                     YDFGMTLFYATPLIFTGLSVAVAFHAGLFNIGAEGQLTLGALAAAAVGAVWPGLWSPL
                     APVLAGLAAILAGTLWGAIPGWL"
     gene            complement(449..2719)
                     /locus_tag="VIL39_02860"
     CDS             complement(449..2719)
                     /locus_tag="VIL39_02860"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY5480396.1"
                     /translation="MKMNSVVTLGANAWHNVRIMKGEQMKRLLVVWMLTLVCVGSVHA
                     GDLTLWYDKPANKGMNEALPIGNGKFGGLVYAGPKQERVVLNESSLWTGTAISTDDYS
                     KMGWYEMLGELLVNLAPDAAAGAVQNYRRSLDLATAVHTVTYQVGGVTFRREAFASHA
                     DQVMVLKFSADRPGACTGTVQLQGAHKEMTAAAGSSLTFAGTLEDGLKYEAKLVALND
                     GGTLQSGDGKLEFKDCNSLTLLVAAGTDYALDYARNYRGENPHAPVEQRLAAAAAKQY
                     DDLKAAHVAEYKSYFNRVALDVGATPADRLALPIDRRKVLHAEGGGDPDLEELLFQYG
                     RYLMISCSRPGGLPANLQGLWNDSNRPPWHSDYHANINIQMNYWPAEPANLAECHTPL
                     FDLIGSQLEPWRKATAAEKRFATAFGKSRGWAVRTSHGINGDEAWQWDVTANAWYCQH
                     FWMHYAFGGDKTWLRSVAYPVMKETCEFWEARLKALPDGRLVIPDGWSPEHGPHEDGV
                     SYSQQIVWDLFNNYVAASEALGMDAAYRANIAALRDKLLGPKIGKWGQLQEWMTDRDD
                     PNDHHRHTSHLFAVFPGNWISTARTPEFAAAAKKSLIARGEEADSDVREWSLAWRCAL
                     YARLHDGESAHRMLQSLLANRNTCLNLFGLHPPMQMDGNFGITAGVCEMLLQSHENEL
                     NLLPALPKCWPAGSIKGLRARGGFEVDIAWKDGQLTQATIRSKLGGPCRLLSGATTAT
                     LATQPGGSYPLNAALR"
     gene            2991..>4485
                     /locus_tag="VIL39_02865"
     CDS             2991..>4485
                     /locus_tag="VIL39_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEY5480397.1"
                     /translation="MIALLLLAVTPHLALAKEFHVSVNGNDNDKGSRSKPFKTISAAA
                     RVAQPGDVITVHAGVYRERINPPRGGKSDTKRIVYQAAAGEKVEIKGSEVVKNWVKVQ
                     EDVWKATLPNAFFGSFNPYNDLIRGDWFNRLGRDHHTGAVYLNGDWLIEAAKLEEVLM
                     PAGTSPAWLAKGRDQYLLNVAWLRPGKGTGNAGRIPATSYAAKNGTQNAPCTEGGECI
                     GFIEHGHWVRYEQVDFGSGTDQMEIRAASASGGGIIEIRLDQPDGELLGTCTVPNTGD
                     WQDWASFQARIKPVSGTRTLCLSFKGPPSAPLNAPLWFAQVDASNTTIWAQFPRVNPN
                     EQLVEINVRRTVFYPDQPGRNYITVRGFTLRHAATPWAPPTSEQVGLIGTHWSKGWII
                     ENNVISHSICSGIALGKHGDQYDNTSADTAEGYVKTIERAHANAIAWTRENIGHHLVR
                     NNTISHCEQAGVVGSLGAAFSTVTGNTIHDIHVRRLFSGAEMAGIKFH"
BASE COUNT          829 a         1432 c         1421 g          803 t
ORIGIN      
        1 ggagccagcc cgggattgcc ccccacagcg ttcccgccag gatcgcggcc aggcccgcca
       61 gcaccggagc cagcggcgac caaaggccgg gccagacggc gcccaccgcc gccgccgcca
      121 gggctcccag cgtgagctgc ccttcggcgc cgatgttaaa cagtcccgcg tgaaaagcga
      181 ctgccaccga aagcccggtg aagatgagcg gggtcgcgta gaatagcgtc atgccaaagt
      241 cgtagctcga gccgaacgcc cccttgccca ggataatgaa aacatgccac gggttctccc
      301 ccgcaatcca cgtcacgccc agtcccaggg caagccccag cgccacggcc aggatgggtt
      361 gaaggagctt gcgcataacg gggccatgtt ctagccgatg ccgccagctt gcggaaggtg
      421 aaattgagcg tcaggaaact tcgtcggctc accgcaaagc ggcgttgagc gggtaactgc
      481 cgcccggctg ggtcgcgagg gtggcagtgg tcgcgccgga aagcaggcgg caggggccgc
      541 cgagcttgga tcggatcgtg gcttgggtca gttgcccatc cttccaggcg atgtccacct
      601 cgaaaccgcc gcgggcgcgg agccccttga tgctgccggc gggccagcat ttcggcaacg
      661 cgggcaatag gttgagttcg ttctcgtgcg attgcagcag catctcgcag acgccagcgg
      721 tgatgccgaa gttgccgtcc atttgcatcg gcgggtggag gccaaacagg ttcaggcaag
      781 tgttgcgatt ggcgagcagg ctttgaagca tccggtgcgc gctttcgccg tcatggagcc
      841 gtgcgtagag cgcgcagcgc cacgccagcg accactcgcg gacgtcggaa tccgcctctt
      901 cgccgcgggc gatcaacgat ttcttcgcgg cggccgcgaa ctcgggggtc cgggcggtgc
      961 tgatccaatt gccggggaat acggcgaaca ggtgcgaggt gtggcggtga tgatcgttgg
     1021 ggtcgtcgcg gtcggtcatc cattcctgga gctggcccca cttgccgatc ttcgggccca
     1081 ggagtttgtc gcgcagggcg gcgatgttcg cgcggtaggc ggcgtccatg ccgagcgctt
     1141 cgctggcggc aacgtagtta ttgaacaggt cccagacgat ttgctggctg tagctgacgc
     1201 cgtcctcgtg cggcccgtgc tccggggacc agccgtccgg gattacgagg cggccgtcgg
     1261 gcagcgcctt cagccgggct tcccagaatt cgcaggtctc tttcatcacc gggtaggcga
     1321 cgctccggag ccaggtcttg tcaccgccga aggcgtagtg catccagaag tgctggcaat
     1381 accacgcgtt ggcggtcacg tcccactgcc aggcttcgtc gccgttgatg ccgtgggagg
     1441 tgcggacggc ccagccgcgt gatttgccga aggcagtggc aaagcgcttc tccgcggcag
     1501 tagctttgcg ccacggttcg agctggctgc cgatgaggtc gaacagcggg gtgtggcact
     1561 cggccaggtt cgccggctcc gcgggccaat agttcatctg gatgtttatg ttcgcgtggt
     1621 aatcgctgtg ccagggcggg cggttgctgt cgttccacaa cccctgcaaa ttggccggca
     1681 acccgcccgg ccgcgaacag gagatcatca ggtaacggcc atactggaaa agcaactcct
     1741 ccaggtccgg gtcaccgccg ccttcggcgt gcaacacttt gcgccggtca atcggcaacg
     1801 cgaggcggtc cgccggggtc gcgcccacgt ccaatgccac acggttgaag tacgacttat
     1861 actcggcaac gtgcgccgct ttcaggtcgt cgtattgctt cgccgccgcg gcggcgagcc
     1921 gctgttcgac tggggcgtgc gggttttcgc cgcggtagtt gcgggcgtaa tccagtgcgt
     1981 aatccgtgcc cgccgcgacc agcagggtga ggctgttgca gtccttgaac tcgagcttgc
     2041 cgtcgccgga ctggagcgtg ccgccgtcat ttagcgccac cagcttcgcc tcgtatttca
     2101 agccgtcttc gagtgtcccg gcaaacgtga gggaacttcc cgccgctgcc gtcatctctt
     2161 tgtgcgcacc ttggagttgc accgtgccgg tgcaggcgcc ggggcggtcc gccgagaatt
     2221 tcaagaccat cacctggtcc gcatgcgacg caaaggcctc ccgccggaaa gtgacgccgc
     2281 cgacttggta agtcacggtg tggacagccg tcgcgaggtc gagggagcgc cggtagtttt
     2341 gaacggcacc ggcggcggca tccggtgcga ggttgacgag gagttcgccg agcatctcgt
     2401 accaccccat tttcgagtaa tcatcggtgg agattgcggt gcccgtccac aggctgcttt
     2461 cgttgagtac gacgcgctcc tgcttcggcc cggcataaac caggccgccg aacttgccgt
     2521 tgccaatcgg cagcgcctcg ttcatgcctt tattggccgg tttgtcgtac cacagcgtca
     2581 ggtcgccggc atgaaccgag ccgacacaga ccagtgtaag catccagaca actaagagtc
     2641 tcttcatctg ttcacctttc atgatgcgga cattgtgcca cgcattagcg cccaaagtca
     2701 caactgaatt catcttcatt tcaccctcta taggtccaca aaacgtgtta ctggagtcac
     2761 aagccgtctc ggatcaccgc ggcatcgggg gtctgcgttg cgtggtggta ccggcaagat
     2821 catcctcggc aaaggagcgg cgcaacttat cgcttgaaat gctcgtcccc ttctgacgcc
     2881 atgtagtcgt aatgatcaag tccgagaacc agttctcctt cacccgcgcc gcggcaataa
     2941 accagcgggg caacgaaaag caacttcctc tcatttaagc tatgaaacag atgatcgcac
     3001 tcctgctgct ggcggtgacg ccgcacctcg ccttggccaa agagttccat gtctccgtca
     3061 acggcaatga caatgacaag ggctcgcggt cgaaaccgtt caagacgatt tcggctgccg
     3121 cccgggttgc gcagccgggc gatgtcatca ccgtgcatgc gggcgtctat cgcgagcgca
     3181 tcaacccgcc gcgcgggggt aaatccgata ccaagcgcat cgtgtaccag gcagcggcgg
     3241 gcgagaaggt cgagatcaag ggctcggagg tcgtgaagaa ctgggtgaag gtgcaggagg
     3301 atgtctggaa agcgacgctc cccaacgcat tctttggcag cttcaatccc tacaacgacc
     3361 tcattcgcgg cgactggttc aatcggcttg gccgggacca tcacaccggc gccgtgtatc
     3421 tcaacggaga ctggctgatc gaggcggcca agctggagga agtcttgatg ccggcgggca
     3481 ccagcccggc ctggctggcg aaggggcgcg accagtacct gctcaatgtg gcgtggctgc
     3541 gacccggcaa aggcacaggc aatgccggcc ggattccggc caccagctat gcggccaaga
     3601 acggcacgca gaacgcccca tgcacggagg ggggcgaatg catcggcttt atcgagcacg
     3661 gccactgggt tcgctatgaa caggtggact tcggctcagg caccgaccag atggagattc
     3721 gcgcggcgtc ggcctctggc ggcggcatca ttgagatccg cctggaccag ccggacgggg
     3781 aattgctggg cacttgcacc gtgccgaaca ccggcgactg gcaggactgg gcttccttcc
     3841 aggcccggat caaaccggtc agcgggacca ggacgttgtg cctgtcgttc aaaggccccc
     3901 cgtcagcgcc gctgaacgcc ccattgtggt ttgcgcaggt ggacgcctca aacaccacca
     3961 tttgggcgca gttcccgcgc gtcaacccga acgagcaact ggtcgagatc aatgtccgcc
     4021 gcacggtgtt ctacccggac cagccgggcc gcaactacat caccgtccgc gggttcacgc
     4081 tgcgccacgc tgccaccccc tgggcgccgc cgacgtccga acaggtcggc ctgatcggca
     4141 cccactggag caagggctgg attatcgaga ataacgtcat cagccactcg atctgctccg
     4201 gcatcgccct cggcaaacac ggcgaccagt atgacaacac ctcggccgac accgccgaag
     4261 gctatgtgaa gaccatcgag cgcgcccatg caaatgcgat cgcctggacc cgagagaaca
     4321 ttggccacca tctcgtccgc aacaacacga tctcgcactg tgagcaggcg ggcgtcgtcg
     4381 gcagcctcgg cgccgcgttc agcaccgtca ccggcaacac catccacgac atccatgttc
     4441 gccgcctctt ctccggggcc gagatggccg gcatcaagtt ccacg
//
LOCUS       DATCGS010000145         4924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_162660, whole genome shotgun sequence.
ACCESSION   DATCGS010000145 DATCGS010000000
VERSION     DATCGS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162660"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /gene="dnaX"
                     /locus_tag="VIL39_02870"
     CDS             <1..1709
                     /gene="dnaX"
                     /locus_tag="VIL39_02870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY5480398.1"
                     /translation="SHAIEQKRIAHAYLFCGPRGTGKTTIARIFAKCLNCADGPKVDF
                     DDNDPRCQEITEGRSLDVLEIDGASNNGVEQVRELRETCKYAPASSKFKIYIIDEVHM
                     LSTAAFNALLKTLEEPPAHVKFMFATTDPEKVLPTILSRCQRFDLRRIPAALIAKHLT
                     HIAGLEQVKIDPAALYAIARGAEGAMRDGESALDQLISFCGDKIEEPDVLSMFGLTAQ
                     GQLHDLSRAVLTGEVEKALRQLNDLANRGKDLGRLLSDLLNHFRNLLIFKVSNGDLTM
                     LEISETEAAALKEQSSLAAADGLTRIMEVLAEAEMRIRDAASKKILVEVAVLKAIEAR
                     GAVSIDSLLKQLQSLRGGAVSEAPRAPAPAATASPRPSRPAAGMASQAPAQQTSVAAP
                     AAVHNTSGGTTPSASDEELAGLWARLLEAVGRASPFTRTYLLEAHPVSFLKGVFTIGF
                     DTEFEDHLGLVDNARNHTLLQTKLTELGHPNAQIKFIKADAPARPMAAPVEPTAPPAP
                     APASSAPTKPASAGKPAVAPAAPAKEKLATVPFNQDDFKNDPLIRKALEIFKGQIVEV
                     RA"
     gene            1748..2059
                     /locus_tag="VIL39_02875"
     CDS             1748..2059
                     /locus_tag="VIL39_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY5480399.1"
                     /translation="MSSIGKLMKQAAHMQQQMKQIQTSLASRTVEATSGGGAVKVVAK
                     CDGTIASIKLNPEALNPGDAQLLEDMILTAANQALGQAKEISNAEMGKATAGFNLPGL
                     T"
     gene            2136..2738
                     /gene="recR"
                     /locus_tag="VIL39_02880"
     CDS             2136..2738
                     /gene="recR"
                     /locus_tag="VIL39_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5480400.1"
                     /translation="MPPLPEPITALVGALSKLPGIGPRSAERIALHLVQAESGVVKQL
                     ADTMLQARERVRLCEVCGALTETSPCTTCTDPRRDASLVCIVERPVDIISLEKSGTFR
                     GQYHVLGGKISPLNGVEPEDLRIGELEKRLTHGPIKEIVIALATDVEGDATGYYLAKR
                     LAREGLKITRIAYGLPAGAGLEFADELTLSRAMEGRREMS"
     gene            2760..3377
                     /locus_tag="VIL39_02885"
     CDS             2760..3377
                     /locus_tag="VIL39_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY5480401.1"
                     /translation="MIIPKAVVFDLGKVLVDFDYSIAARRIAARGKMTLLEIAAYINQ
                     SPLFAEYELGHVTTQQFYDEIRKVTGFRGDLAEFSGCFADIFTEIKPMVQIQAELQQR
                     GMPAYVFSNTNELAAEHIRRNFPFYSRFDGYILSYEHGAMKPDARLYEVVERQSGQRD
                     AEILYLDDRPENIAAGAARGWQVILQESPEKSRAAVQKLGLLNHG"
     gene            3434..4309
                     /locus_tag="VIL39_02890"
     CDS             3434..4309
                     /locus_tag="VIL39_02890"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5480402.1"
                     /translation="MESATARIPTRSHRQAMDWSLVLVSQGIETTIDVAEDGGGWGLL
                     VAGQDYGPAIRAIRQYHIENRGWPWQQQVFRPGFLFDWGALAWVLLAGMFFWLNTSTD
                     LKTAGTMDGAAVAHGQWWRLFTAIWLHADLAHLATNASIGLILLGLTMGRYGTGVGLL
                     AAYLAGAGGNLLAGLLSLQTHRSLGASGMVMGSLGLLAVQSLSLWRQTPHATKFILSG
                     LCGGVMLFALLALTPGTDVMAHLGGFVSGLLLGALFNLVPAIAQKPAANLLGGLVFAL
                     LVIVPWWLALRSPAP"
     gene            4395..4628
                     /locus_tag="VIL39_02895"
     CDS             4395..4628
                     /locus_tag="VIL39_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480403.1"
                     /translation="MPQLERKISLPANRLVVNVDCSEYFNTLDPAQQPRKTGTWCHSW
                     QIADQLDRGAHRGGDEHLDGLGKQRAPENLVRF"
BASE COUNT          968 a         1578 c         1514 g          864 t
ORIGIN      
        1 tctcccacgc gatcgagcag aagcggatcg cgcacgcgta cctcttttgc ggcccgcgcg
       61 gcaccggcaa gaccaccatc gcccgcatct tcgccaaatg cctcaattgc gcggacggcc
      121 cgaaggtgga ttttgacgac aacgaccctc gctgccagga aattaccgag gggcggtcgc
      181 tggacgtgct tgagatagac ggcgccagca ataacggcgt cgagcaggtg cgtgagttgc
      241 gagagacctg caagtacgcg ccggccagct ccaagttcaa gatttacatc attgacgaag
      301 tccacatgct ctcgaccgcg gcgttcaacg cgctgctcaa gacgctggag gagccgccgg
      361 cacacgtgaa gttcatgttc gcgacgacag acccggagaa ggttctgccg acgatccttt
      421 cgcgctgcca acggtttgac ctgcggcgca tcccggcggc gctcatcgcc aaacatctga
      481 cgcacattgc cgggttggag caggtgaaga ttgacccggc ggcgctctac gcgatcgccc
      541 gcggcgccga gggagccatg cgcgacgggg aatcggccct cgaccagctt atcagcttct
      601 gcggcgacaa gatcgaggaa ccggacgtgc tgtctatgtt cggtctgaca gcgcaaggcc
      661 aactgcacga cctttcccgg gccgtgctga cgggcgaagt cgagaaggct ttgcgccaat
      721 tgaacgacct ggccaaccgc ggcaaagacc tgggccgcct gctgtccgat ctgctgaacc
      781 atttccgcaa cctgctcatt ttcaaggtgt cgaacgggga cctgaccatg ctggagatat
      841 ccgagaccga agccgcggca ctcaaggagc agtcttccct ggccgccgcg gacggactga
      901 cgcgcatcat ggaggtcctg gccgaggcgg aaatgcgtat tcgcgacgcg gcttcgaaga
      961 agatactggt cgaagtggcc gtgcttaaag ccatcgaagc ccgaggcgcg gtgagcattg
     1021 attcactcct caagcagctc cagagtctgc gcggcggcgc agtgagtgaa gctcctcgcg
     1081 cacccgcgcc tgcggcgacg gccagcccga ggccctcacg accagcagcc ggcatggcga
     1141 gccaggcacc ggcgcaacaa acctctgtgg ccgcgcctgc cgcagttcat aacacgtctg
     1201 gcggcacgac tccgtcagca tcggacgaag agctggccgg gctatgggcc aggctgctcg
     1261 aggcggttgg ccgcgccagc ccgttcacgc gcacttacct gcttgaggcg cacccggttt
     1321 cattcctcaa gggtgtattc acgattggct ttgacaccga attcgaggat cacctgggcc
     1381 tggtggataa cgcccgcaac cacactttgc tccagaccaa gctgaccgaa ctcggtcatc
     1441 cgaacgccca gatcaagttt atcaaagccg acgcgcccgc caggcccatg gcggcgcccg
     1501 ttgagcccac agcgccgccc gcacccgcgc cggcgagcag cgctcccacc aaacctgcca
     1561 gcgccggcaa acccgccgtt gcgcccgctg cgccagccaa agaaaagctg gccaccgtgc
     1621 cgttcaacca ggacgacttc aagaacgatc ccctgatccg gaaggcgttg gaaatcttca
     1681 agggccagat cgtcgaggtc cgcgcctgac tctcatccgt taaccatcaa ccattacctc
     1741 ttcaactatg tccagcattg gcaaactcat gaaacaggct gcgcacatgc agcagcagat
     1801 gaaacagatc cagacctcac tggccagccg cacggtcgaa gccaccagcg gcggcggggc
     1861 cgtaaaggtc gtcgcgaagt gcgacggcac gattgcctcg atcaagctca acccagaagc
     1921 gctgaacccg ggagacgcgc agttgctcga agacatgatc ctgaccgcgg ccaaccaggc
     1981 cctgggccag gccaaggaga tttccaacgc cgaaatgggc aaagccaccg ccggcttcaa
     2041 tctccccggc ctgacgtgac agttgagggc tgacgtttga gagtcgcggg tccgcaccgc
     2101 cctcacgcat cacgcatcac acatcacgtc cccccatgcc ccccctcccc gagccaataa
     2161 ccgccctggt cggcgccttg agcaaactgc ccggcatcgg gccgcgctcg gcggaacgga
     2221 ttgccctgca cctggtgcaa gccgagtccg gcgtcgtcaa acagctcgcc gacaccatgc
     2281 tccaggcgcg cgagcgggtc cgcctctgcg aggtctgcgg cgcgctgacg gaaacgtcgc
     2341 cctgcactac ttgcaccgat ccgcgccggg acgcgtcgct ggtctgcatc gtcgagcggc
     2401 cggtggacat tatcagcctg gagaagtccg gcacgtttcg cggccaatac cacgtgctgg
     2461 gcggcaagat ctcgccgctc aatggggttg agcccgaaga cctgcgcatc ggcgagctgg
     2521 agaagcgcct gacgcacggg ccaatcaagg agatagtcat cgccctggca acggatgtgg
     2581 agggcgacgc gacggggtat tatctggcca agcggctggc gcgggagggt ttgaagatca
     2641 cgcgcatcgc ctacgggcta cctgccggtg cggggctgga attcgctgac gagctgacct
     2701 tgagccgagc catggaagga cgaagggaaa tgtcgtgagc gcgaaactga atggatgaca
     2761 tgattattcc gaaagcagtc gtctttgacc tgggcaaagt gctggttgac ttcgactact
     2821 cgattgccgc gcgccggatc gccgcacggg gcaagatgac cctcctcgag attgcggcgt
     2881 atatcaacca gtcgcccctg ttcgctgagt atgagttagg ccacgtcacc acgcagcagt
     2941 tctacgacga gatccgaaag gtcaccggct tccggggcga cctggcggag ttctcaggct
     3001 gcttcgccga catcttcaca gagattaaac ccatggtgca gatccaggca gagctgcagc
     3061 agcgggggat gcccgcttac gttttttcga atacgaacga gctggccgcc gagcatattc
     3121 ggaggaactt cccgttttat agtagatttg acggctacat tctgtcgtac gaacacggcg
     3181 cgatgaagcc ggacgccagg ctttacgagg tggtcgaacg ccagtctggc cagcgggatg
     3241 cggagattct atacttggac gaccgcccgg agaacatcgc ggcgggcgcg gcgcgcggct
     3301 ggcaggtcat cctgcaggaa tctcctgaga agagccgggc cgccgttcag aagctcgggc
     3361 tactgaacca cggctagacc gtagtttacc gcgacagtga tggccggatg accgcggcca
     3421 agttgaatca caaatggaat ctgcgactgc caggatccct acgcgttcac accgccaggc
     3481 gatggactgg agccttgtgc tcgtgagcca gggcatcgag acgacgattg acgtggccga
     3541 ggacggcggc gggtggggtc tgttggtggc agggcaggat tacggccccg ccatccgggc
     3601 gatccgccag tatcacatcg agaatcgagg ctggccgtgg cagcagcagg tgttccggcc
     3661 gggcttcctt ttcgactggg gcgccctggc ctgggtgttg ctggccggca tgttcttctg
     3721 gctcaacacc agcaccgacc tgaaaaccgc cggcacgatg gacggcgcag ccgtcgcgca
     3781 cgggcaatgg tggcggcttt tcacggccat ctggctccac gccgacctcg cgcacctggc
     3841 gaccaacgcg agcatcggcc tgatcctgct cggcttgacg atggggcgct acggcacggg
     3901 ggtgggattg ctcgcggcct acctggcggg ggccggcggc aacctcctgg cggggctcct
     3961 gtctttgcag acgcaccgaa gcctcggcgc atccggcatg gtcatgggca gccttggatt
     4021 gttggcggtg cagtcgttgt cactgtggcg gcagacgccc catgcgacga agttcatcct
     4081 gagcgggctc tgcggcgggg tgatgctgtt cgctctgctc gcattgaccc caggcacgga
     4141 tgtcatggcc cacctgggcg gattcgtaag cgggttgctg ttaggcgccc tgttcaacct
     4201 cgttccagcg attgcgcaaa agccagccgc caatctcctc ggcggcctgg ttttcgccct
     4261 cctggtcatt gtgccctggt ggctggccct ccggagccct gctccctagc acgaggactg
     4321 gtgtccggca acgcggccaa tccctcggaa catcgctggc agatctcctt gcgcccacaa
     4381 aaggccgagg cgttgtgcct cagttggaaa ggaaaataag cctcccggcc aaccgtctgg
     4441 tcgtcaatgt ggactgctcc gaatacttca acaccctcga ccccgcgcaa cagccccgta
     4501 aaaccggcac ctggtgtcat tcctggcaga ttgccgatca gcttgaccgc ggcgcccatc
     4561 ggggtggcga tgagcacctg gacgggctcg ggaaacaacg ggccccggag aatcttgttc
     4621 ggttttaggt cttctgactt cacggtactt ggtcctgctc caacagattc ctagggtatg
     4681 gtttacagtg atccgccagg aagtgcacga agattcttga cagcaattct cattatggcc
     4741 ttggccaacc cccgccgggt gagagcaccc ggcctacacc cttacaaaac cgccctcttg
     4801 taggccgggt gccctcaccc ggcggcccga tgtggtccat aatgagaatt gctgacgcct
     4861 cccccaggaa aatccaaaac ttgtaatacc ggctggtttc ggcgaaaaca ctggcgcatg
     4921 tcac
//
LOCUS       DATCGS010000146         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_657490, whole genome shotgun sequence.
ACCESSION   DATCGS010000146 DATCGS010000000
VERSION     DATCGS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657490"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VIL39_02900"
     CDS             <1..1094
                     /locus_tag="VIL39_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEY5480404.1"
                     /translation="PRRDFLKLCLTATAMMGLPLSMHSQVAAAAQSSAKPPVIWLHFQ
                     ECTGCSESLLRSSHPTISSLILDLISLDYHETLMAGSGAQAEQSLHESMAANRGKYIL
                     VVEGAIPTAENGIFCKVGGKTAMESLREAAPGAAAVLCIGTCSSFGGIQSVRPNPTGA
                     VGVRDLVTDRPIVNLPGCPPNPYNFLSTVLHYLTFKRLPELDALGRPKFAYGRRIHEH
                     CERRPHFDAGRFAQAYGDAGHAQGYCLYKLGCKGPATYANCSVQRFNDVGVWPVSIGH
                     PCIGCTEPDILFKLPIAEKVQIHNPTPFDSYAPVNLKEKGKGADPVTTGIVGLAAGAV
                     IGAGIMLAKKLPNNTPDHPGDPHHHEPAK"
     gene            1078..2007
                     /gene="hybA"
                     /locus_tag="VIL39_02905"
     CDS             1078..2007
                     /gene="hybA"
                     /locus_tag="VIL39_02905"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEY5480405.1"
                     /translation="MSQQSRREFFKAAGLASAGLVAAATPASASSSEHLPDNDQLGML
                     YDATKCVGCKACMSACKRVNARSGGLDFDQARFDQDGLWDAPEDLDGNTRTLIKLFKN
                     AKGWSYIKYSCMHCQKPSCVSVCPVRAMTKDPVTGIVNYSKDVCIGCRYCQVACPFNI
                     PKFQWDRTVPRIVKCNLCRDTNLKDKGIPACAEVCPAGAILFGKRKDLLAEAHARLQQ
                     YPKRYVPHIYGEHEIGGTNHLYLAALPFERLGLPTLSTEAPASFSERIQHTIYKGFIA
                     PIALYASLCFIALRNRKKGEVQPAPTAPPKKDA"
     gene            2008..>2489
                     /gene="nrfD"
                     /locus_tag="VIL39_02910"
     CDS             2008..>2489
                     /gene="nrfD"
                     /locus_tag="VIL39_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5480406.1"
                     /translation="MAHDEYQKQEGRILTPSFFVLLSLSLVGIFLILVRCVKGIGAVS
                     NLSDGYPWGLWITYDVAVGTAIACGGYAVAILIYIHNHMRYHPLIRSCVLTSMFGYGL
                     AGFSVMVDLGRPWNAYNFFVPSKWQANSAMFEVALCVMAYATVLIIEFLPAILYSLET
                     "
BASE COUNT          480 a          850 c          679 g          480 t
ORIGIN      
        1 tgccgcggcg ggactttctc aagctgtgcc tgacggccac cgccatgatg gggctgccgt
       61 tgagcatgca cagccaggtc gctgccgccg ctcaatccag cgccaagccg ccggtcatct
      121 ggcttcattt ccaggaatgc accggctgtt ccgaatctct cctccgctcc agccatccca
      181 ccatctcatc gctcatcctg gatctgatct cgctggatta ccacgagaca ctcatggcgg
      241 gctccggcgc ccaggcggaa cagtcgctgc acgaatcgat ggccgctaat cgcggcaagt
      301 atatactggt agtcgaaggc gctatcccga cagccgaaaa cggcatcttc tgcaaggtgg
      361 gcgggaagac ggccatggaa tcactgcgtg aggccgcccc gggtgcggct gccgtgcttt
      421 gcatcggcac ctgctcaagc tttggcggca tccaatccgt gcggcccaat ccgaccgggg
      481 cagtgggcgt gcgggacttg gtcaccgacc ggccaatcgt caaccttccc ggttgtcctc
      541 ccaaccctta caatttcctc tccacggttc tccactatct cacgttcaaa cgcctgcccg
      601 agctcgatgc gcttggccgg cccaagtttg cttatggccg caggattcac gagcattgcg
      661 agcggcgtcc gcacttcgac gccggacgat ttgcccaggc gtatggcgat gccggccacg
      721 cccagggcta ctgcctctac aaacttggct gcaagggccc agccacctat gccaactgct
      781 ccgtccagcg cttcaacgac gtgggagtat ggcccgtctc gatcgggcat ccctgcatcg
      841 gctgcaccga accagacatc ctcttcaagc tccccatcgc cgagaaggtc cagattcaca
      901 atccgacgcc ttttgacagc tatgcgccag tgaacctgaa ggagaagggc aaaggggcgg
      961 acccggtcac tacaggcata gtcgggctgg ccgccggcgc agtcatcggc gccggcatca
     1021 tgctggcaaa gaaactgccg aacaacaccc ccgatcaccc aggcgatccc catcaccatg
     1081 agccagcaaa gtaggcgcga attcttcaaa gccgccggac tggccagcgc gggtttggtc
     1141 gcggcggcca ccccagcctc ggcctcgtcg tcggagcacc tgccggacaa cgaccagttg
     1201 ggcatgcttt atgacgccac caaatgcgtc ggctgcaagg cctgcatgtc cgcctgcaag
     1261 cgcgtcaatg cccgctcagg tgggctcgac ttcgatcagg ccaggtttga tcaggacggc
     1321 ctgtgggacg ccccggaaga cctggacggc aacacccgca ccttgatcaa gctgttcaag
     1381 aacgcgaagg gctggtctta catcaagtat tcgtgcatgc attgccagaa accctcctgc
     1441 gtttctgtct gccccgtccg cgcgatgacc aaggacccgg tgaccggcat cgtcaattac
     1501 agcaaggacg tttgcatcgg ctgccgttac tgccaagtcg cgtgtccttt caacatcccc
     1561 aagttccagt gggatcgcac agtgccgcga atcgtaaagt gcaacctctg ccgggacacc
     1621 aacttaaaag acaaaggcat accggcgtgc gccgaggtct gtcccgccgg ggcgatcctc
     1681 tttggcaagc gcaaagacct gctggcagaa gctcacgccc gcctccagca gtacccaaag
     1741 cgatacgtgc ctcacattta cggcgaacac gagatcggcg gcaccaacca cctctacctg
     1801 gccgccctgc ccttcgaacg gctcggcttg cccacgctct cgaccgaagc cccggcctcg
     1861 ttctcggagc gaatccagca caccatctac aagggcttta tcgcgcccat cgcgctctac
     1921 gcttccctgt gctttatcgc cctccggaac cgcaagaagg gcgaagtgca acccgcgcca
     1981 acggcgcccc ccaaaaagga cgcatagatg gcccacgacg aatatcaaaa gcaggaaggc
     2041 cgaatcctca cgccgtcctt tttcgttctg ctaagcctct ccctggtggg gatcttcctg
     2101 atcctggttc gctgcgtcaa aggcatcggc gcggtctcca acttgagcga cggctatccc
     2161 tggggcctct ggattaccta cgacgttgcc gtcggcaccg ccatcgcctg cggcggctac
     2221 gcggtggcca tcctgatcta tatccacaac cacatgcggt atcacccgct gattcggtcg
     2281 tgcgttctga ccagcatgtt cggttacggg ttggctggct tctcggtcat ggtggacctg
     2341 ggccgcccct ggaacgccta caatttcttc gtcccgtcca agtggcaggc caattccgcc
     2401 atgttcgagg ttgcgctttg cgtcatggcc tacgcgaccg tgctcatcat tgagtttctg
     2461 ccggccattc tctacagcct ggaaaccag
//
LOCUS       DATCGS010000147         5023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_459683, whole genome shotgun sequence.
ACCESSION   DATCGS010000147 DATCGS010000000
VERSION     DATCGS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459683"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..686)
                     /locus_tag="VIL39_02915"
     CDS             complement(60..686)
                     /locus_tag="VIL39_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5480407.1"
                     /translation="MPITVSIVEDNDQLRGTLARVIGRAEGFKCVSHYPNAEAALAGL
                     PKDSPEVVLMDINLPGMNGVECVRQLKQVAPQIQSVMLTVYEDTENIFAALAAGASGY
                     LLKRTKTAELLDAIREVHRGGSPMTTHIARKVTQSFLKAGSSPQPTENLSQREQEVLD
                     CLSNGFLYKEIADKLGISYETVHTYIRRIYEKLQVRTRTEAVAKFLRR"
     gene            complement(809..1915)
                     /locus_tag="VIL39_02920"
     CDS             complement(809..1915)
                     /locus_tag="VIL39_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY5480408.1"
                     /translation="MLSATFDSQIWSAVPFHFWSVVFFVFGCIVGSFLNVCIYRMPLE
                     QSIVSPPSHCPHCQYSIPWYLNIPLVTWLYLRGKCRNCGAPISIRYFLVELLTGITFL
                     SCWLTFGSESAWLALVYALFLAGLITATFIDFEHFIIPDEITVGGTVAGFVCSFLLPS
                     LHSETVLSGSLRQSVLGIVAGAGIIYCILRLGKLIFGRQKVTLPAETRIIFTENAVHL
                     PDEEIPYDDLFYRPSDAITFQAHTVELVDRCYKDVRVRLTPVSLRIGEENLNPEEQPH
                     LEAVTTDIVLPREVMGLGDVKFMAAIGAFLGWKAVVFSLMVSSMIGSVVGVTLIVLGK
                     REWSSRLPYGPYIALAAATWVFGGKRLVQWWFGM"
     gene            complement(2028..2945)
                     /locus_tag="VIL39_02925"
     CDS             complement(2028..2945)
                     /locus_tag="VIL39_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5480409.1"
                     /translation="MSGPFSQPIDASTRICAVYGHPVRHSASPAMQNAGLAALGLNWR
                     YLAFEVDPDSLRAAIQGAKAMNFVGLNLTVPHKLLAVDMVDVLDDSAKSWGAVNTIRF
                     EGREERGAWRPLREFAEAPRETRAHGFNTDAGAITRALCEDLGLELSGAKILLLGAGG
                     AGRTAALKLASERISELWLVNRTRSKADAVAKEIRERHPGAKVTTGYPAGPVDLVLNA
                     TSLGLKPNDPSPLDGKQFTLRQAGAVYDMIYRPAETALLKEAKAAGCRTANGLGMLLY
                     QGAEALELWSGQTAPVEIMRQALKRNVYG"
     gene            3064..3663
                     /locus_tag="VIL39_02930"
     CDS             3064..3663
                     /locus_tag="VIL39_02930"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5480410.1"
                     /translation="MATLVIKSAEFGNRVIELNLGVNRFGRSPENAVQIEHPTVSATH
                     CEIVLADDGVVVRDCDSANGTYVDAQLVKEARLFAGQTLRLGEIELLVETTDVTIAIP
                     QFDMPRPAPPVVLSDGSLICPRHPKARATHQCTHCREVLCDACVHHLRRRGGKLMKLC
                     PLCSHPCLPLGAEKRKKKSFFGLWRKTTKLPFFKKPKQL"
     gene            3718..>5023
                     /locus_tag="VIL39_02935"
     CDS             3718..>5023
                     /locus_tag="VIL39_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5480411.1"
                     /translation="MRLQLSLLMLLGTCFASLAQETLPTTQPLTLQGDLSAQMVAGID
                     RFLTRRTAESITERQKYWQRDFSSVEAYDASVQTNREHLRMIIGAVDARLPVTALEFV
                     SSTTSPAKVGETELFTVEAVRWPVFEGVYAEGLHLQPKGDPVACIVAIPDADQTPEML
                     VGLTPGLAPARQFARRLAENGCEVLVPVLIDRQDTWSGSVQIKRFTNQPHREWIYRQA
                     YELGRHIIGYEVQKVAAAVDFFESRKRKAESRNLKIGVAGYAEGGLIAFYAAALDRRI
                     EATMVSGYFDSRQALWEEPIYRNVFGLLHEFGDAEIASLIAPRALIVEYSAAPKVEGP
                     PPPREGRTGAAPGKLQTPDYSSVEAEFERARALLKPDQHKDFDRFKLISGTEGMSTGP
                     GSDRALTAFLNALGVSAHEPSSSGAGVSPAAAGVSPAVESSRA"
BASE COUNT          998 a         1581 c         1529 g          915 t
ORIGIN      
        1 agattggtca accggggacg aggccgtccc cgccccttca tgaattggcc ctgggcgggc
       61 taacgccgca ggaacttggc gaccgcctcg gtccgggtcc ggacctgcag cttctcgtaa
      121 atgcggcgga tgtaggtgtg caccgtttcg tagctaatgc ccagcttgtc ggcgatttcc
      181 ttgtagagga agccgttgct caggcaatcg agcacctcct gctcgcgttg cgagagattc
      241 tccgtcggtt gcggcgacga tccggccttt agaaaagact gcgtcacctt ccgcgcgatg
      301 tgcgtcgtca tcggcgagcc tccccgatgc acttcacgga tggcgtcgag caactccgcg
      361 gtcttcgtgc gcttgagcag gtatcccgac gctcccgccg ccagcgccgc gaagatgttc
      421 tccgtgtcct catacaccgt cagcatcacc gactgaatct gcggcgcgac ctgcttcaac
      481 tgccgcacgc actccacgcc attcatcccc ggcaggttga tatccatcag caccacttcc
      541 ggactgtctt ttggcaagcc cgccagcgcc gcctcggcat tggggtagtg gctgacgcac
      601 ttgaatcctt ccgcccggcc gatcacgcgc gccagcgtgc cgcgcaactg gtcgttatct
      661 tccacgatgg aaactgtgat tggcatggtt ccgtttggcc cggacgacga tcatccgccg
      721 tccgccacgc ccactcctac ccgccccgac taccgccgtc aaatcaatag ctcaatcaat
      781 acggcccatt ttgagcgata agagctattt acatcccgaa ccaccactgg accaggcgtt
      841 tgccgccaaa cacccaggtc gcggcggcca gcgcgatata aggtccgtaa ggcaggcggg
      901 aggaccactc gcgcttgccg agcacaatca aggtcacacc gaccacagag ccgatcatcg
      961 aactgaccat cagggagaaa accaccgcct tccacccgag aaacgcgccg atggcggcca
     1021 tgaatttcac gtcgcccaga cccatcactt cccgcggaag cacaatatcg gtggtcaccg
     1081 cctccagatg gggctgctcc tccgggttca ggttctcctc gccaatccgc aggctgacgg
     1141 gagtgagccg cactcgcacg tctttgtagc agcggtccac caattccacc gtgtgcgcct
     1201 ggaaggtgat ggcgtccgaa gggcggtaga agaggtcatc gtaggggatt tcttcgtctg
     1261 gcaaatgcac cgcgttctcg gtgaagatga ttctggtctc ggcgggcagc gtcacctttt
     1321 ggcggccgaa gatcagtttg cccaggcgga ggatgcaata gatgataccg gcacccgcca
     1381 cgatgccgag cacgctttgc cgcagcgagc cggacagcac ggtctccgag tggaggctcg
     1441 gcagcagaaa agagcagacg aatccggcca ccgtcccgcc gacggtgatt tcatcgggga
     1501 taatgaagtg ctcgaagtca atgaacgtgg cggtgatcaa gccagccagg aacagggcgt
     1561 aaaccagcgc gagccaggcg gactcgctcc cgaacgtcaa ccagcagctc agaaaggtta
     1621 tgcccgttag cagctcgacc aggaagtagc ggatggaaat aggcgcgccg cagttccggc
     1681 atttcccgcg cagatacagc caggtgacta gcgggatatt cagataccac gggatcgagt
     1741 actggcagtg cggacagtgg gagggcgggg agacgatgct ttgctccagc ggcatccggt
     1801 agatgcagac gttgaggaag ctcccaacga tgcagccgaa cacaaagaac accaccgacc
     1861 agaaatggaa cggcacagcc gaccagattt gtgagtcaaa ggtcgcggaa agcatgcggg
     1921 gctaaaatcc gaaggccgaa ggccgaaacc cgaaggaaac ccgaaggccg aactccgaag
     1981 agcggttgca gtgtccccag gtttggtgaa ttccgtatgg gcggttgtca gccatagaca
     2041 ttccttttta acgcttggcg cataatctct actggggcgg tttgcccgct ccacaattcc
     2101 aaagcctcgg cgccttggta caacaacatg ccgagaccgt tggcggtgcg gcagcctgcg
     2161 gctttggcct ccttgagcag ggcggtctca gccgggcggt aaatcatgtc ataaacagca
     2221 cctgcctgcc gcagcgtaaa ctgctttccg tccagaggcg acggatcgtt cggcttcagg
     2281 ccgagcgacg tggcgttcag caccaggtca acgggtcccg ccgggtagcc tgtcgtcacc
     2341 tttgcgcccg ggtggcgctc gcggatttcc tttgcgacgg cgtccgcctt gctgcgcgtg
     2401 cggttaacga gccacaactc cgagatgcgt tccgaggcga gtttcagcgc ggcggtccgc
     2461 cctgccccgc cggccccgag cagcaggatc ttcgcgccgc tcaactccaa ccccaaatct
     2521 tcgcacagag cgcgcgtgat cgcgccggcg tccgtgttaa atccgtgcgc gcgggtttcc
     2581 cgcggcgcct ccgcaaactc ccgcagcggc cgccacgcgc cgcgctcctc ccggccctcg
     2641 aagcggatgg tgttcaccgc gccccagctc ttggccgaat cgtcgagcac gtcaaccata
     2701 tccaccgcca gcaacttgtg cggcacggtg agattgagcc cgacgaagtt cattgccttg
     2761 gcgccctgga tggccgcgcg gaggctgtcg gggtccactt cgaacgccag atagcgccag
     2821 ttcagcccga gcgccgccaa gccggcattc tgcatcgcgg gcgacgccga gtgcctgaca
     2881 ggatgcccgt acacggcgca aatgcgcgtg ctggcatcaa tcggttgtga gaacggcccg
     2941 gacatgcgct ggttataggc aaggcagggc gaagttcaaa gagcaaaatg aaaggccccc
     3001 gcatattctt atggacattc cccgggtggg gccgtatcta cgcgacgact gaacagagca
     3061 gaaatggcca cgctagtcat taaatccgcc gagtttggca accgggtgat tgaacttaac
     3121 ctgggcgtca accgatttgg gcgcagcccg gagaatgctg ttcagattga gcatcccacg
     3181 gtgtcagcca cgcattgcga aatcgtgctc gcggacgacg gggtcgtggt gcgtgactgc
     3241 gactcagcca acggcacgta cgtggacgcc cagcttgtca aggaagccag gctgttcgcc
     3301 ggccaaactc tgcgcctggg agagattgaa ctgctggtgg agaccacgga tgtcaccatc
     3361 gccattcccc aattcgatat gcctcgcccc gcgccacccg tcgtcctatc ggatggctcc
     3421 ctgatttgtc cccgccaccc caaagcgcgc gcaacccatc agtgcaccca ttgccgtgaa
     3481 gtcctgtgcg acgcttgcgt gcaccacctc cggcgtcggg gcgggaagct catgaagctc
     3541 tgcccgctct gcagccaccc ttgcctgccg ctcggcgccg agaaacggaa gaagaaatcg
     3601 tttttcggcc tttggcgcaa gaccaccaag ctgcccttct tcaagaagcc aaagcagctt
     3661 tgactcgaca cggatcgtcc gatggaagaa tctcggcagc gctgtgcgcc tgctcaaatg
     3721 agactgcaac tgtccttgtt gatgctgctg ggaacttgct ttgccagcct ggcgcaggag
     3781 acccttccca ctacgcagcc gctgaccctc cagggcgacc tctccgccca aatggtcgcc
     3841 ggcattgacc gattcctcac gcgccggacg gcagaatcaa tcacagaacg gcagaaatac
     3901 tggcagcgcg acttctcttc cgttgaagcc tacgatgcct cagtccagac gaatcgcgag
     3961 cacctgcgca tgattattgg tgcggtggat gcgcgcctcc cggtaacggc gcttgagttt
     4021 gttagcagta ccaccagtcc ggcgaaggtt ggcgagacgg aactcttcac ggtggaagcg
     4081 gtgcgctggc cggtgtttga aggtgtgtac gccgagggcc tgcacctcca gcccaaaggc
     4141 gaccccgtcg cctgcatcgt ggcgattcct gatgccgatc agacgccgga gatgctggtc
     4201 ggactcacgc cgggactggc gccggcacgc cagttcgcac gccgcctggc cgagaacggc
     4261 tgcgaggtcc tcgtgccggt attgattgat cggcaggaca cctggtcggg aagcgtccag
     4321 atcaagcgct ttaccaacca accgcatcgc gaatggattt accgccaggc ctacgagctg
     4381 ggccggcaca tcatcgggta tgaagtgcag aaggtggcgg cggcagtgga cttctttgaa
     4441 agcagaaagc ggaaagcaga aagcagaaat ctcaagatcg gcgttgctgg gtatgcggag
     4501 ggaggattga tcgcgttcta tgccgcggcg ctggatcggc ggattgaggc gacgatggtg
     4561 agcggctact tcgattcgcg ccaagctctc tgggaggagc ccatttaccg gaatgtgttc
     4621 gggttgttgc atgagtttgg cgatgcggaa atcgccagtc tcatcgcccc gcgcgccttg
     4681 attgtggaat acagtgccgc gccgaaggtc gagggaccgc cgccgccccg cgaaggccgg
     4741 acgggggccg cgcccgggaa gctgcagacg cccgattact catccgtgga ggccgagttc
     4801 gaacgggcac gagcgctgct caagcccgac cagcataaag acttcgaccg gttcaagctt
     4861 atcagtggca ctgagggcat gtccaccggg ccgggttcgg accgggcgct gacggccttc
     4921 cttaacgcgc tcggcgtctc tgcgcacgag ccttcaagta gtggggcggg cgtctcgcct
     4981 gccgcagcgg gcgtctcgcc cgcggtggaa agttctaggg cga
//
LOCUS       DATCGS010000148         3646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_63600, whole genome shotgun sequence.
ACCESSION   DATCGS010000148 DATCGS010000000
VERSION     DATCGS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63600"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1541
                     /locus_tag="VIL39_02940"
     CDS             117..1541
                     /locus_tag="VIL39_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480412.1"
                     /translation="MKQNKMSQAGVSRRQFIGSTAAAVAGFSLVPRHVLGGPKFVAPS
                     EKINIAIIGCGGQGRTNVRTLFNQPDVQIIAVADPIESENLDGFYFKGNAGRLPVKAE
                     IEKHYSEKTPNFKVADYEDFRTMLEKEKSIDAILCATPDHLHAYVCVTAMRQGKHVYC
                     EKPLTHNVWEARQVAKVAKETGVATQLGNQGHSGDFIRQTCEMIWDGAIGDVHEVHAW
                     TSASRWNKQILSGRPAAEPVPKGVNWDLWLGPRETRPYSSAYSPVSWRDFWDFGTAPI
                     GDFFCHNFDPACWALDLREPLSVEASAAGGVDSYIAPPGGLYTYHFGPRGKMPAVKFV
                     WYEGGLMPERPEGLEDDDQLGANGNGILFVGDKGMITCGGWAGRPSLLPGSKDEAYKR
                     PAKTLPRSKGHHRDWLDACKGGKPASANFEYGAALTEVGLLGLVALRLGKKMYWDAKA
                     MKATNAPAADALLKERYRPGWEVT"
     gene            1664..2644
                     /locus_tag="VIL39_02945"
     CDS             1664..2644
                     /locus_tag="VIL39_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480413.1"
                     /translation="MAQHISRRGFVKSSLLASAAVPLGLSGPTRSTAQTSATPAGSTV
                     TPPQETLPMGKMGGQEFSRLIMGGNLFGGWSHSRELAYVSNLMRRYNTDAKIRETLEL
                     AEAQGITAINTYVMQENAQLFNHWKGGGKMKWFAQVRLDSNGGYSQIQKAADEGAAGI
                     HVNGDASESILNEGKFEKIGEMVQMIKSKKRAAGVGAHDLRVIVECEKAKLDVDFYQK
                     TFHSHEYFTAPRPGEKDAVGPHDNSWCSDPQAVIDFMATVKKPWVAFKILAAGAIAPR
                     AAFPYALNNGADFLLVGMFDWQIEEDVKLARRVFAAATSPDSKRTRPWYG"
     gene            2692..>3646
                     /locus_tag="VIL39_02950"
     CDS             2692..>3646
                     /locus_tag="VIL39_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5480414.1"
                     /translation="MNINLLRPLFLCGLACWALGFPALTADQPQWGQAWSRNMVSDER
                     GLPDSFDPESGRNIKWSAELGTETHSTPVVARGRVYIGTNNGHPRDPAQQGDRGILMC
                     FDEQTGRFLWQLVVPKRTEDIYFDWPNSGIASPATVEGDRVYLVSNRGEVLCLDARPV
                     ARKSETRNPKAEMNPVPSGLEPHVIWRFDLTTGAGIWSHDAAHSSILIHGDQLYLNTG
                     TGVDNTHRHIRTPDAPSLVVLDKRTGRLIARDDEHIAPNIFHSTWSAPSLATVNGRPL
                     FFFAAGNVIVFAFETVRNGVLGTNSSPQYSTTPTLRFSDESV"
BASE COUNT          732 a         1219 c         1070 g          625 t
ORIGIN      
        1 ttcttcggct tctatcccgc ccgcaaagcc tcacagcttg atcccattga cgcgctgcgc
       61 tatgagtgaa gcggcacagg ctgttacaga aacaactcgg ttacatcaat ccaactatga
      121 aacagaacaa aatgtcccag gccggcgtga gccggcgcca attcatcggc agcacggcgg
      181 cggcagtggc cggctttagc ctggtcccac ggcatgtcct cggcggcccg aagttcgtcg
      241 cgcccagcga gaagattaat atcgccatca ttggctgcgg cggacagggg cgaacaaacg
      301 tccgcaccct gttcaatcaa ccggatgtgc agatcatcgc ggtggctgac cccatcgagt
      361 ccgagaacct ggatggcttc tatttcaagg gcaacgcggg ccgcctgccc gtgaaagcgg
      421 aaatcgagaa gcactactcg gaaaagacgc ccaacttcaa ggtggctgac tacgaggact
      481 tccgcacgat gctggagaaa gagaagtcca ttgacgccat cctttgcgcc acgccggatc
      541 acctgcacgc ttacgtctgc gtgaccgcga tgcgccaggg caaacatgtc tattgcgaaa
      601 agccgctcac ccacaacgtc tgggaagcgc gccaggtcgc caaggtggca aaagaaaccg
      661 gcgtggcgac ccagttgggg aaccagggcc actccggcga cttcatccgg cagacctgcg
      721 agatgatctg ggacggggcg attggggatg tgcacgaagt ccacgcctgg accagcgcca
      781 gccgttggaa caaacagatt ctcagtggcc ggccagcggc ggagccggtc cccaaagggg
      841 tgaactggga tttgtggctg ggaccgcggg agacgcgacc ctacagttca gcctactcgc
      901 ccgtgtcctg gcgggacttc tgggacttcg gcaccgcgcc catcggagac ttcttctgcc
      961 ataactttga tccggcctgc tgggcgctgg acctgcgcga acccctcagt gtcgaagcgt
     1021 ctgctgccgg cggcgtggac tcgtatattg cgccgccggg aggcctctac acctaccact
     1081 tcggtccgcg cggcaagatg cctgcggtca agttcgtctg gtatgaaggc gggctgatgc
     1141 cggagcgccc cgagggtctg gaggacgatg accaactcgg cgccaacggc aacggcatcc
     1201 tgttcgttgg cgacaaaggc atgatcacct gcggcggctg ggcgggaagg ccctccctgt
     1261 tgcccggctc caaagacgaa gcctacaagc gcccggcaaa gaccttgccg cggtccaaag
     1321 gccaccaccg cgactggctc gacgcttgca aaggcggcaa accagccagc gccaattttg
     1381 aatacggcgc tgccttgacg gaagtcggcc ttctcggcct ggtagctctg cgtctcggca
     1441 agaagatgta ttgggatgcc aaggcaatga aagccaccaa tgccccggcg gccgacgccc
     1501 tcctgaagga acgctatcgc cccggctggg aagtgacata agtcgaacgc gacgagagcc
     1561 atgcaggcgg tcttcccggc gagcccgcgc caagggagcg aaacctaacc tcagcctgcg
     1621 gccagccttg acactcgccg ccgaaatggt tacgcgttat ctcatggcac aacatatttc
     1681 gcggcgcgga ttcgtgaaat cctcgctcct cgcttccgct gcggttccgc tgggtctgag
     1741 cggcccaacc cggtccaccg cccaaacttc cgccaccccc gccggttcga ccgtcacccc
     1801 gccccaggag accctgccga tgggcaagat gggaggccag gagttcagcc gcctgatcat
     1861 gggtggcaac ctcttcggcg ggtggtcgca ctcgcgcgaa ctcgcctacg tctccaacct
     1921 gatgcgccgc tacaacaccg acgccaagat ccgggagacg ctcgaattgg cggaggccca
     1981 gggcatcact gctatcaaca cctatgtcat gcaggagaac gcccagctct tcaaccactg
     2041 gaagggcggc ggcaaaatga aatggtttgc ccaggtccgc ctcgactcga acggcggcta
     2101 ttcccagatc cagaaggcgg ccgatgaggg cgccgcgggc atccacgtta atggcgacgc
     2161 gtccgagtcc atactcaacg aaggcaagtt tgagaagatc ggcgagatgg tccagatgat
     2221 taagtccaag aagcgcgccg ccggcgttgg cgcccatgac ctgcgcgtca ttgtcgagtg
     2281 cgagaaggcc aaactggacg tggacttcta ccagaagacc tttcattcgc acgaatactt
     2341 cactgctccc cgccccggtg agaaggatgc cgtaggccct cacgataatt cctggtgcag
     2401 cgacccgcag gcggtgattg acttcatggc gaccgtcaag aagccctggg tcgccttcaa
     2461 gattctggcg gccggtgcca tcgcaccgcg cgccgcgttc ccctatgcct taaacaacgg
     2521 cgccgacttt ctcctcgtcg gcatgttcga ctggcagatc gaggaagacg tcaagctggc
     2581 ccgccgcgtc ttcgccgccg ccaccagccc cgactccaag cgcacccgcc cctggtacgg
     2641 ctgagcggtt tgccgggcgc cggcgtctgc ccctgagctc cagcgcaacc gatgaacatc
     2701 aacctcctgc gcccgctgtt cctgtgcggc ctggcatgct gggccttggg cttcccggcc
     2761 ctgaccgccg accaaccgca atggggccag gcttggtccc gcaacatggt gtcggacgag
     2821 cgcggactgc ctgattcttt tgatcccgag agcggacgga acatcaaatg gtccgcggaa
     2881 ctcggcacgg aaacccactc cacgcctgtg gtcgcgcgtg gtcgcgtgta catcggcacc
     2941 aacaacggcc acccgcggga ccccgcgcag caaggcgacc gcggtatcct gatgtgcttc
     3001 gacgaacaga cgggccgttt cctctggcaa ctcgtcgtcc ccaaacgcac cgaggacatc
     3061 tacttcgact ggccgaatag cggcattgcc tcgcctgcca ctgttgaggg tgaccgcgtc
     3121 tatctcgtca gcaaccgcgg cgaggtcctc tgcctcgatg cgcggccggt ggcgaggaaa
     3181 tccgaaaccc gaaacccgaa ggccgaaatg aatcccgtgc ccagcgggct cgaaccacac
     3241 gtcatctggc ggttcgacct caccacgggc gcgggcatct ggtcgcacga tgctgcccat
     3301 agctccatcc tcattcacgg cgaccagctt tacctgaaca ccggcaccgg cgtggacaac
     3361 acccaccgcc atatccgcac gcccgatgcg cccagcctag tcgtgctcga caagcgcacg
     3421 ggccgactca tcgcccgcga tgacgagcac attgccccga acatctttca cagcacctgg
     3481 tccgccccgt cgctggccac cgtgaatggc cgcccgctat tcttcttcgc cgccggcaac
     3541 gtgatcgtgt ttgcgttcga gacggtgcga aatggagtgc tgggaacgaa ttcttccccc
     3601 caatactcca ccactccaac actccgtttc tctgacgaaa gtgtgg
//
LOCUS       DATCGS010000149         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_657732, whole genome shotgun sequence.
ACCESSION   DATCGS010000149 DATCGS010000000
VERSION     DATCGS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657732"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..>2535)
                     /locus_tag="VIL39_02955"
     CDS             complement(142..>2535)
                     /locus_tag="VIL39_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5480415.1"
                     /translation="PGVEGSTGSGFVPPPVADIAKLFPQLEVLEFIGKGGMGAVYKAR
                     QPNLDRLVALKILPPTSASDPGFAERFNREARALARLNHPNIVAVHDFGQAGMLHYLV
                     MEYVDGGNLRQIERAGRLTPEQALAIVPQICEALQFAHNEGIVHRDIKPENILLDKKG
                     RVKITDFGIAKMLGLTAEEAPLTGAGDVVGTPHYMAPEQIEKPRSVDHRADIYSLGVV
                     FYEMLTGDLPLGKFAPPSRKVQVDVRLDEVVLQALEKEPNLRYQQASQVKTAVETIAN
                     SQGRGPQADEQAAKPDAEALERDVLARDYVLDIGSCLHRGWALVRSDFWPLVGITALM
                     LALLSAVSSVGEVSRSVGNVHFNTSVLGILLGGPLLGGLYLYFLKKIRDEQVRVETAF
                     SGFSNSLLHLILASFVTGMLTTLGFLCLILPGIFLFVAWFFTLPLVIDQRLDFWPAMR
                     LSLRTIIKHWWKFLGFLIILALFNLGGVLLCFVGVFITLPVSIAALMYAYEDIFNPPG
                     LPTAPPVGSGVPGVGSEAQAYVTAQPHGLGSWATLFLTGAVIILLLAFAAMAGLTGKL
                     IGYYLALAAGALALAIFLRVRKSALPDGFMATFLLVFLLVLGAAVFITSILPESFIST
                     TRVKLTPNTTEASQTSGTQSASGTYDPYLIQTECEVMQSEEILGSVIQALHLERTWGQ
                     RYGNDRPLKLAETMDLLKSRMVVRPLRNTSLISISIYADQPEEAARIANEIVQTYKDR
                     DNLGPFQVEVVDRAMPALRPIRPNKPFNLALGVLLGLVLGTGAGAARVARHARKTLN"
BASE COUNT          515 a          777 c          802 g          441 t
ORIGIN      
        1 acgactcatc caggaaggtc tcgacagcgg accggctgga ccgctgaacc gccgcacctg
       61 ggagcaaatc cgggaggaga gcgacaggtt cgcccgtacc ctgcgccggc accaaaaacg
      121 cgccgcctga cgtgctgccg ctcagttcaa ggtcttcctg gcgtggcgag caacgcgggc
      181 tgcgccggcg cccgtcccga gcaccagccc caacaggaca cccagcgcaa gattgaatgg
      241 tttgttaggc cggatgggtc tgagggcggg cattgcccga tcaactactt cgacctggaa
      301 ggggccgagg ttgtcccggt ccttgtaggt ttggacgatc tcgttggcga tccgtgcggc
      361 ttcttcaggc tggtcggcat agatgctgat tgagatcagg ctcgtgttgc gcagcgggcg
      421 gaccaccatt ctgctcttga gcaggtccat ggtttcggcc agcttgagcg gccgatcgtt
      481 gccgtagcgc tggccccaag tccgctccag atggagtgct tggattacgg agccgagaat
      541 ctcctcagat tgcatgacct cgcattcagt ctggatcaga taaggatcgt aagtccccga
      601 cgcgctctgc gtgccggatg tttgcgaagc ctcggtcgtg ttgggcgtga gcttgacgcg
      661 ggtcgtgctg ataaacgact ccggcaaaat ggaggtgatg aaaacagcag ccccgagcac
      721 cagcaggaag accaacagga aggtcgccat aaaaccatcc ggcaaggccg atttgcggac
      781 tcgcaggaaa atggccaggg cgagggcgcc cgccgctaaa gccaggtaat agccgatgag
      841 cttgcccgta aggccggcca tggcggcaaa ggccagcaaa agaattatga ccgcgccggt
      901 gaggaacagg gtcgcccacg agcccaggcc gtgcggttgc gctgtcacgt aagcctgcgc
      961 ttcgcttccc acgcccggca cgccgctccc aaccggcgga gcggttggca gccccggcgg
     1021 attgaagatg tcttcgtagg catacatcag cgcggcgatt gacaccggga gcgtgatgaa
     1081 gacgccaacg aagcagagca gcacgccccc cagattgaac agcgccagaa tgatcaggaa
     1141 gcccaggaac ttccaccaat gtttgatgat ggtcctgagg ctcaaccgca tggccggcca
     1201 gaagtcgagc cgctggtcaa tgaccagggg cagggtgaag aaccacgcaa cgaagaggaa
     1261 gatgccgggc agtatgaggc agagaaaccc gagcgtcgtg agcatcccgg taacgaagct
     1321 ggccaggatg agatggagga gggaattgct gaagccggag aaggcggttt cgacgcgcac
     1381 ctgctcgtct cgtatcttct taagaaagta tagatacagt ccgcccagga gcggaccgcc
     1441 cagcaggatg cccaggacgg aggtgttgaa atgcacgttt cctacggagc gcgatacttc
     1501 gccgactgaa ctcaccgccg acaacagcgc cagcatgagc gcggtgatgc cgacgagcgg
     1561 ccagaaatca ctccgcacca acgcccagcc gcggtgcagg caactgccga tgtccaacac
     1621 ataatcccgg gccagaacgt cccgctccag cgcttccgcg tcgggctttg cagcttgctc
     1681 atcggcctgc ggacctcggc cctgggagtt ggcaatggtc tcgaccgccg tttttacctg
     1741 gctggcctgc tggtagcgca ggttcggttc tttctccaac gcctggagca ccacctcgtc
     1801 cagccgcaca tccacctgca ccttgcggga gggcggggcg aatttcccca acggcaggtc
     1861 gccggtgagc atctcgtaga aaacgacacc cagggaataa atgtccgccc ggtggtccac
     1921 gctccggggc ttctcgattt gctccggggc catgtagtga ggggtgccga ccacatcccc
     1981 ggctcccgtt agcggagcct cttcggcggt gaggccgagc attttggcga tgccgaagtc
     2041 ggtgatcttc acccggccct tcttatcgag gagaatgttc tcgggcttga tgtcacggtg
     2101 gacgatgccc tcgttgtgcg cgaattggag cgcttcacag atttgcggga cgatcgccag
     2161 tgcctgctcg ggggtgagcc ggccggcgcg ctcgatctgc cgcaggttcc cgccatccac
     2221 atattccatc accaggtagt gcaacatgcc cgcctgccca aaatcatgca ccgcgacgat
     2281 gttcggatgg ttcaggcgtg ccagggcgcg agcttcccga ttgaaccgct cagcaaagcc
     2341 aggatcgctg gcggacgtcg gcggcaggat cttcagcgcc accaaccggt ccaaatttgg
     2401 ctggcgcgcc ttatagacag cgcccatgcc gcccttgccg atgaactcaa ggacttcgag
     2461 ctggggaaac aacttggcga tgtccgcgac cggcggcggc acgaaccccg acccggtgga
     2521 gccttcgacc cctgg
//
LOCUS       DATCGS010000150         5511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_311492, whole genome shotgun sequence.
ACCESSION   DATCGS010000150 DATCGS010000000
VERSION     DATCGS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311492"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1938
                     /locus_tag="VIL39_02960"
     CDS             46..1938
                     /locus_tag="VIL39_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480416.1"
                     /translation="MPTNERTPLPALRAHITAASSLPWGGLLVVLFLLQGWCPSMAKA
                     DETPVVRVFDTRATSRAPLSSDAVCNHRNWTLVPEDTTAHKFKGDLVLLNSKLAVVLR
                     RSGRGAEVYSLESNGATLRAELVALGSDPSAALSAVKIVENTADVASVDATYKTSKGG
                     RLTLGYELKQGQVFVQTQPRSGATGLQVNAPCRFAVLPDFFADDMVVDATQLMVPQTE
                     LPSENFLLHMLADGEAILMAEWYPAKDDVRIMLSGHQQDRRITSSEIHYAPEGKICVA
                     VLSAPGIWHRRDILPGDADRILHLGWKAPYAAHWRVDWQRDDKLTDSWEMLTQKPDGE
                     YVKHGWYGQSESFGNIDWLKDGRKRWTTVLGSFLYPCWVDKNGEGYLQPLKERVRFEG
                     PALIYPINRLEATPLEQFTVVDIMRATMGVGPCEYILDVEGQKKTFQGRPTCASRTIL
                     DNIYASKAQKEKREEVQRTLDEVIAFVRHIRGRINDYAAFGHEMVAWLEQQKATHPEL
                     TGILNEMQDTTRKIDGFVDRRKEGIKTPEYATQLVDEFRSTLVDYEGEDALAKCKKIT
                     AALVGIGGNQDELVGECRMVVKILRQKAAMAMAGDPRTATVAREVRRRTQQMLRNPTS
                     YEAPRH"
     gene            1939..2853
                     /locus_tag="VIL39_02965"
     CDS             1939..2853
                     /locus_tag="VIL39_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY5480417.1"
                     /translation="MKELETPIHSGPSLNSQAATRQLLGRAVWVLALFSLTACNQGPK
                     TEPAAEVKSAAAKPITTLGGLTLALIPAGEFEMGDNKGDPDERPAHKAQVSAFYMDTH
                     EVTQKAYETLMQQNPSKSKGPDKPVEQVDWYHAVLFCNMRSLKEGLKPCYDSKTLACD
                     YNANGYRLPTEAEWEYACRAGTRESYSFGNDAAKLKACAWFKANAGQTPHPVGQKSPN
                     AWGLYDMHGNVAEWCQDFYSETFYSKSPGKDPRGPTSGDKCVLRGGSWRTSEDGCRSA
                     ARNSETLRFADACFGSDAFGFRCVKSAE"
     gene            2868..5183
                     /locus_tag="VIL39_02970"
     CDS             2868..5183
                     /locus_tag="VIL39_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480418.1"
                     /translation="MRANSVILVALLALAGFGAGMIIGGRLVSPKASTGAPMPTVGTP
                     GGDHLPAIKPRPKPGASGPSAGAPESASLAEIEAALQKALRISSGRGYKALNELVQKV
                     NPSDIPQLLAFAEKLPSTNQKSQLRSLLLARWAETDVSAAMAYADAVTGYQNRQQAIL
                     AVLNAWVEKDAEAASAWAQQLPPGQLRKQALNAVSYGLALKDPEAAYRLMTTSNAGDR
                     RWGMTYEIFSAWAANDPAGAAAKAAQLTSAQQRQQAFQVIAARWGEQDPQAALAWAKG
                     LPDGRDKSSALSSVIGQWAATDPKSAAVTALSLPAGQSRNNAISSVASQWANTDLSGA
                     VAWVQQLPEGQPKQNALSSVTWQWVQADPQAAMAYAQSLPAGQTRDNMMSNLGNRLAE
                     QDPQAALTWAQSLPAGQSRNQILERVVSGLAWSDPKTAAAFAASLPPGQMRNQALNQV
                     VNGWAQVDVKEATEWVNTLPDGQAKRDAQRNLSYQMAESDPQAAADYALSLPAGQARN
                     DFISRVASSWGNADLTAAVAWVQQLPDGQAKQNAMNSMSYQWVQADPQGAAAYAQSLP
                     TGEMQNNLVNTVANQWAATDPKAAVAWASQFPEGETRNRALAGVTSGWAQNAPQEAAG
                     YVAALPDGEARNRATLSVVTQWAHSEPDAAAAWVAGFGEGKLRENAARELVSNWARED
                     STAAGQWLQTLPAGASRESAVQAYVNDLASSSPELAAPWAESITDQNIRDNQIENIAR
                     NWLQADRPAAEAWLAKVNLPDDRKQRLLKQP"
BASE COUNT         1157 a         1780 c         1693 g          881 t
ORIGIN      
        1 cggtccgaac gagtgaatta ctgaatagat gatgttatga tttccgtgcc aacaaacgaa
       61 cgcacgccgc ttccggcatt acgcgcccac ataactgcgg cgagcagtct cccctggggc
      121 ggcctcctgg tggttctatt cctgctccag ggctggtgcc cttccatggc gaaggccgat
      181 gaaacgccgg tcgtgcgagt gtttgacacg cgcgcaacct cgcgggctcc gttgtcctcc
      241 gacgcagtct gtaaccatcg caactggaca ctggtgccgg aagacacaac ggcccacaag
      301 ttcaaaggcg atcttgtcct cctcaacagc aagctggccg ttgttctgcg ccgcagcggc
      361 cgcggcgcgg aagtctatag cctcgagtcg aacggcgcga cgctgcgcgc ggagctcgta
      421 gcgctgggta gcgatccctc cgccgcgctc tccgcagtta agatcgtgga gaatacggcg
      481 gacgttgcat ctgtggatgc gacttataag acctccaagg gcggacggct cacgctcggc
      541 tacgaactca aacagggcca ggtgttcgtg caaacgcagc cccgttccgg agcgaccggc
      601 ctgcaggtga atgcgccttg ccgcttcgcg gtgctgccgg acttctttgc cgacgatatg
      661 gtggtggatg ccactcaact gatggtgccg caaaccgagt tgccgagcga aaactttctc
      721 ctgcacatgc tcgctgacgg ggaggcgatc ctgatggcgg aatggtaccc ggcgaaagac
      781 gacgtgcgca ttatgctctc cgggcaccaa caagaccgac gcatcaccag ttcggagatt
      841 cattacgcgc cggaggggaa aatctgcgtc gccgtcctga gtgctcccgg catttggcac
      901 cggcgcgaca ttcttccagg ggatgctgac agaatccttc accttggctg gaaggctccc
      961 tatgccgctc actggagagt ggactggcag cgcgacgaca agctgaccga tagctgggag
     1021 atgttgacgc agaaacccga cggcgaatac gtgaagcacg gctggtacgg ccaatcagag
     1081 tcgttcggga acattgattg gctcaaggac ggccgaaaga gatggaccac ggttctcggc
     1141 tccttccttt atccctgctg ggttgacaag aatggcgagg gatacctgca acctctcaaa
     1201 gagcgtgtcc ggttcgaggg cccggccctg atttacccca tcaaccgcct ggaagcgacg
     1261 ccgctcgagc agttcacagt cgtggacatt atgcgcgcga cgatgggggt cggcccttgc
     1321 gaatatattc tggatgtcga gggacagaag aagacgtttc agggccgtcc cacgtgcgcc
     1381 tcgcggacca tcctggacaa catctacgcg agcaaggcac aaaaggagaa gcgcgaggaa
     1441 gtccaacgaa cactcgatga agtcatcgcc ttcgtccgcc acatccgcgg cagaatcaac
     1501 gattatgccg ctttcggcca tgagatggtt gcctggctgg agcagcagaa ggcaacgcac
     1561 cccgagttga ccggcatcct gaacgagatg caagacacca cacgcaagat agacgggttt
     1621 gttgatcgcc gcaaagaagg gatcaaaacg cccgagtatg cgacccaact cgtggatgag
     1681 ttccgcagta ccttggttga ttacgaaggc gaggacgcac tggccaagtg caagaagatc
     1741 accgccgcac tagtgggtat cggcggcaac caggacgagc tcgtcggcga gtgccggatg
     1801 gtggtcaaaa tcctgcgcca aaaggcggct atggctatgg ccggcgaccc tcggacagcc
     1861 acggtcgccc gagaagtgcg ccgtcgcacc cagcaaatgc tccgtaaccc cacaagctac
     1921 gaagctccga gacactgaat gaaagaactc gaaaccccaa ttcactctgg accctccttg
     1981 aattcccaag cagcaacccg tcagcttttg gggagggctg tctgggtcct ggccctgttc
     2041 agcctcacgg catgcaacca gggcccgaaa accgagcccg ccgccgaagt gaaatcagcc
     2101 gcagcgaaac cgatcacgac gctgggcggc ctcaccctgg cgctcattcc tgccggcgag
     2161 ttcgagatgg gggacaacaa gggcgacccg gacgaacggc ccgctcacaa agcacaggtg
     2221 agcgcatttt acatggacac gcacgaggtg acgcaaaaag cctacgagac tctcatgcag
     2281 caaaacccat ctaaatcaaa agggccggac aagccggtcg agcaagtgga ctggtaccat
     2341 gcggtcctct tttgcaacat gcgttcgctc aaagaagggc tgaagccgtg ctacgactcg
     2401 aagactttgg catgtgatta taacgcgaac ggctaccggc tccccacaga agcagaatgg
     2461 gaatatgcct gccgcgcggg gacgcgggaa agttattcgt tcggcaatga cgcagccaaa
     2521 ctcaaggcct gcgcctggtt caaagcaaat gccggtcaga caccacaccc cgtcggacaa
     2581 aaaagcccaa atgcatgggg cctttatgac atgcacggca acgtcgccga gtggtgccag
     2641 gatttctaca gcgagacctt ttactcgaag agcccaggca aggatcctcg cggcccgacc
     2701 agcggcgata aatgtgtgct gcgcggcggc agttggcgca ccagcgagga cgggtgccgc
     2761 tctgccgccc gaaatagtga aacgcttcgt tttgccgacg cgtgtttcgg gtctgacgcc
     2821 ttcggcttta ggtgtgtcaa gagcgcagaa tagcagcggt taaagttatg agagcaaata
     2881 gcgtcatcct cgttgcgctc cttgccctgg ccggtttcgg cgccgggatg atcatcggcg
     2941 ggcggctcgt gtcgcccaag gcatccacgg gggctcctat gcccaccgtc ggcactccgg
     3001 ggggcgacca cttgccagct atcaagcctc ggcctaagcc cggcgcgtcc ggcccgagtg
     3061 cgggcgcgcc cgagtcagcc tcattggccg agatcgaagc ggcgctccag aaggccctcc
     3121 ggataagcag cggccggggc tacaaggctc tcaacgagct cgtgcagaag gtcaatcctt
     3181 cggacattcc ccaattactg gccttcgccg agaagctccc ctccacgaac caaaaatccc
     3241 agttgcgctc tctgctgctg gcgcgttggg ccgaaaccga tgtctccgcc gcgatggctt
     3301 acgcggacgc cgtcaccggt tatcaaaacc ggcagcaggc catcctcgcg gtgctgaacg
     3361 catgggtgga gaaggacgcg gaggcggcca gcgcctgggc gcaacagctt ccgcccggcc
     3421 agctacgcaa gcaagcgctc aacgccgtca gctacggcct ggccctgaag gacccggaag
     3481 cggcctaccg gctgatgacc acttcgaatg cgggcgaccg ccggtggggc atgacgtatg
     3541 agatcttcag cgcctgggcc gccaacgacc ccgcgggcgc cgccgctaaa gccgcccagc
     3601 tcacttctgc ccagcaacgc cagcaagcgt ttcaggtcat tgccgcgcga tggggtgaac
     3661 aggacccgca ggcggcgctg gcctgggcga agggtttgcc cgatggccgc gacaagtcga
     3721 gcgcgctctc cagcgtcatc ggccagtggg cggcgaccga tcccaaatcc gccgcagtta
     3781 ccgccctctc cctgccggcc ggccagtcgc gcaacaacgc cattagctcg gtggcctcgc
     3841 aatgggccaa caccgacctg tcgggtgcgg tagcctgggt gcagcagttg cccgaaggcc
     3901 agccgaagca aaacgcgctg agcagcgtca cctggcaatg ggtccaggcc gatccccagg
     3961 cggccatggc ctatgcgcag agcctgcccg ccggccagac ccgcgacaac atgatgagca
     4021 acctcggcaa tcggttggcg gagcaggacc cgcaggccgc tctcacctgg gcgcagtcac
     4081 tgcccgccgg ccaaagccgc aaccagatcc tggagcgcgt tgtttccggg ctggcctgga
     4141 gcgatcccaa gactgccgcc gcctttgccg ccagcctccc cccggggcaa atgagaaacc
     4201 aggctctaaa ccaggtggtc aacggctggg cccaggtgga cgtcaaagaa gccaccgaat
     4261 gggtcaacac cctgccggat gggcaggcca agcgcgacgc gcaacggaat ctttcctatc
     4321 agatggccga gagcgacccg caagcggcgg ccgattacgc cctgtcgctg ccggcggggc
     4381 aggcgcgcaa cgacttcatc agccgcgtcg cctcctcctg gggaaacgcg gacctgaccg
     4441 ccgcagtggc ctgggtccaa caactccccg acggccaggc caagcagaac gccatgaaca
     4501 gcatgagcta ccaatgggtg caggccgacc cccagggcgc ggccgcctat gcccaaagcc
     4561 tgccaacagg tgaaatgcaa aacaacctcg tcaacaccgt tgccaaccaa tgggcggcga
     4621 ctgatccgaa ggctgccgtt gcctgggcca gccaattccc cgaaggcgaa acccgcaacc
     4681 gtgccctggc cggtgtcacc tccggctggg cgcagaacgc gccgcaagag gccgccggct
     4741 acgtcgccgc cctcccggac ggtgaagccc ggaaccgcgc gacgctctcg gtcgtcaccc
     4801 aatgggccca cagcgaaccg gacgccgccg ccgcctgggt ggccggtttc ggcgaaggca
     4861 agctgcgcga gaacgcggcc cgcgagctag tctcgaactg ggcgcgggaa gattcgaccg
     4921 ccgccggcca gtggctgcag acgttgcccg ccggcgcctc gcgcgaatct gccgtccagg
     4981 cctacgtcaa cgaccttgcc tcctcctcgc ccgaactggc cgcgccctgg gccgaaagca
     5041 tcaccgacca gaacatccgc gacaaccaga tcgagaacat tgcccgtaat tggctccaag
     5101 ccgaccgccc cgccgccgaa gcctggctcg ccaaagtcaa ccttcccgac gaccgcaagc
     5161 agcgtttgct caagcagccg tgagcgagta gttggtaggg acgcctcgcc gaggcgtccg
     5221 cgttggggtg ggagtggacg gctcggcgag ccgtccctac ccaccttacc aaggacttgc
     5281 gaatgcgccc gaggatgccg cgtccttgcc ccccagcttc cgcgacgctg gcctcgggcc
     5341 ggcggcaagg gtgatgcccc cgtgatgctc cggtgatgct ccgaaggagt tacgatgggg
     5401 cttcgaaggg gttacgatgg ggttacgaag ggaatcaagg gaaatcagcc ctctgttacg
     5461 ggtgtgcaaa aggtgagatg ccgccgaatt ggcgcggcca ttgacaaaat c
//
LOCUS       DATCGS010000151         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_209178, whole genome shotgun sequence.
ACCESSION   DATCGS010000151 DATCGS010000000
VERSION     DATCGS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209178"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VIL39_02975"
     CDS             <1..581
                     /locus_tag="VIL39_02975"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5480419.1"
                     /translation="VVITAVARDDVPDGGADHFRRTIEAVRALNPATVIEVLTSDFNA
                     HEAAIGTVIAAQPHIFNHNLETVRRLTPVVRSRATYDRSLSVLGHVKAKCDGRIYTKS
                     GLMLGLGETEEELFIALADLRRVGCDLLTLGQYLQPTLKHLPVVEYVTPEKFAEYGAR
                     AREMGFRHVASAPMVRSSYHADEFTLPASAMQ"
     gene            complement(567..1190)
                     /locus_tag="VIL39_02980"
     CDS             complement(567..1190)
                     /locus_tag="VIL39_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480420.1"
                     /translation="MSSSKYRVRRATLDDIGQLTAIWESMHYPAQDLARRITEFQVAE
                     GADGTVLGGVGLQVAERQGLVHSEAFGDFALAEPLRQLLWDRVNAVATNRGLLRLWTQ
                     ENAPFWNHCGLVQADAEALQKLPALWRGPSSAWLTLKLKDDVDSVLTADREFAVFMQS
                     EKQRTARVFQRARVIKAIATVIAFALLIAVVAWGVRMYLRNPQLLHR"
     gene            1520..>2162
                     /locus_tag="VIL39_02985"
     CDS             1520..>2162
                     /locus_tag="VIL39_02985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5480421.1"
                     /translation="MRYRILGSTGLRVSVIGLGTWQLGGEWGRTFSQADADAILDKAA
                     ELGINLIDTAECYGDHLSEGLIGDYLSRHDRSRWLVATKFGHHFNKFLDRTDGFSAAD
                     VRKQLEASLRALRIEAIDLYQFHSGSDSQLLNEDLWAMLAEQKRAGKVRHLGISILGK
                     GSEAQAREARRLGVEALQVIYNRLDRRPEELYFSQAREHNLGILARVPLASGLL"
BASE COUNT          415 a          680 c          649 g          418 t
ORIGIN      
        1 atgtcgtcat cacggcggtg gcccgcgacg acgtgccgga tggcggcgcg gaccatttcc
       61 ggcgcacgat cgaggcggtg cgcgcgctaa atccagcgac cgtcattgaa gtgctgacct
      121 cagacttcaa cgcgcatgaa gccgccatcg gcactgtgat cgccgcccaa ccgcacattt
      181 tcaatcacaa cctggaaaca gtgcggcgcc tgacccccgt ggtgcgctcg cgagcgacct
      241 acgatcgttc gctcagcgtg ctgggccacg tgaaggcgaa gtgtgatgga aggatttaca
      301 ccaagtccgg cctgatgctg ggcttgggcg agaccgaaga agaactcttc atcgccctgg
      361 ccgacttgcg ccgcgtcggc tgcgacctgc tcacactcgg ccagtatctt cagccgacgc
      421 tgaagcattt gccggtggtg gagtacgtga cgccggagaa gttcgcggaa tacggcgcgc
      481 gcgcgagaga gatgggtttc cggcacgtgg ccagcgcccc gatggtccgc agctcctacc
      541 acgcggacga gttcaccctt cccgcctcag cgatgcagta gctgcgggtt gcgcaggtac
      601 attctgacgc cccaagccac caccgcgatc agcaatgcga aggcgatcac tgttgcgatg
      661 gccttgataa cccgtgcccg ctgaaagacc cgcgccgtgc gctgcttttc ggattgcatg
      721 aacacggcga attccctgtc cgccgtcagg acgctatcca catcatcttt caacttcaag
      781 gtcagccagg cggacgatgg cccgcgccac aaagcaggca gcttttgcag cgcctcagcg
      841 tcggcctgga ccagcccgca atgattccag aacggggcgt tctcctgcgt ccacagccgc
      901 agcaagccac gattcgtcgc cacggcattg acgcggtccc acaatagctg gcgcagcggc
      961 tcggccagcg cgaagtcgcc aaaggcctcg ctgtgaacca ggccctggcg ctcggcaact
     1021 tgcaggccca ccccgcccaa tactgtgccg tccgcgccct cggcgacctg gaattccgta
     1081 atacgcctgg ccaggtcctg ggccgggtag tgcatggatt cccatatagc cgtcaactgg
     1141 ccaatgtcat ccagcgtcgc gcgccgcacg cggtattttg acgagctcac gccgccgaat
     1201 ttaggattca gcgcattaaa ttcaaggccc aaaaggggca aaacggcctt ctcgcccgta
     1261 catttcataa cggaaccagt gagtgcatgg tgcgataata aacgtgggct tcgtgccgtg
     1321 tatggagtcc cggctttagc cggcagggtg ctgtcccttg aagggggctc gaaacacttg
     1381 gagatccatg tcgaaaccgc gtctgaccgg ctaaagccgg gactccatac actgtgccct
     1441 tcgttcgcct gtgaacaatc atccggcatg ccctgatgat tcgagtttca gaaactgggt
     1501 tttgcggtaa gcttccccca tgcgctatag aattctcggc agcacgggtt tgcgggtgtc
     1561 ggttattggc ttgggcacgt ggcagttggg cggcgaatgg ggccgcacct tttcccaggc
     1621 cgacgcggac gctatcctcg ataaggcggc tgaactcggc atcaacctta ttgacaccgc
     1681 agagtgctac ggcgaccacc tttccgaggg tctgatcggc gattacctta gccggcacga
     1741 tcgctcgcgc tggctcgtgg caaccaagtt cggccatcac ttcaacaagt tcctggaccg
     1801 caccgatggt ttctcagcgg cggacgttcg caagcagctt gaagcgtcgc tgcgggcatt
     1861 gcgcatcgag gccattgacc tctatcaatt ccattccggc tcagacagcc aactgctgaa
     1921 tgaggatctg tgggcgatgc tcgctgagca gaagcgcgcg ggcaaagtcc ggcatctggg
     1981 catttccatt ctcggcaaag gcagcgaggc gcaggcacgg gaagcgcggc gtctgggcgt
     2041 tgaggccttg caggtgattt acaaccgcct tgatcgccgg cccgaggagc tctatttttc
     2101 gcaggcgcgg gagcataatc tcggcatcct ggcgcgcgtc ccgctggcca gcggcctctt
     2161 ga
//
LOCUS       DATCGS010000152         3924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_163334, whole genome shotgun sequence.
ACCESSION   DATCGS010000152 DATCGS010000000
VERSION     DATCGS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163334"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VIL39_02990"
     CDS             complement(<1..233)
                     /locus_tag="VIL39_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480422.1"
                     /translation="MEVFYTSPWVPPEWIKAHGLEPRGIWLARDFACEPLPLAAGVCA
                     FANAAVRLAERQTERAVVFTTHCDQLRRGFDAVP"
     gene            complement(233..1456)
                     /locus_tag="VIL39_02995"
     CDS             complement(233..1456)
                     /locus_tag="VIL39_02995"
                     /inference="COORDINATES: protein motif:HMM:NF040772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HEY5480423.1"
                     /translation="MVDDTCQRLAAARNEPPGPLAWFAGMIDHCYDYAEAARGAGRPI
                     VGIMCEFTPRELILAAGGVPVCLCGGSAATIPAAEQYLPANLCPLIKSTFGYHVTGKN
                     PFLKWADLIVAETTCDGKKKMFELMGASKPMYVLELPQKAEDPDALEHWVLEVRKFQQ
                     HLATRFATDITEARLIQAIQLMNRERGLRRRLADLMMLETPPLTGRQLLDFKSTISGI
                     EADLQQYDCALQMYAPVSGNAKAPNHASRITHHASRPPLRVLLTGVPLAHGAERVLEL
                     IEGCGAVVVCMENCTGLKPILEDVDLDSEPDPIQAIAKKYYHLPCSVMTPNPRRFDSL
                     RVLAAKFRPQCVIELVWQACLTYDVESYQVRKLVEEELRLPYLKITTDYSPSDSARIT
                     ARVEALFEMVRAKAG"
     gene            1630..>3924
                     /locus_tag="VIL39_03000"
     CDS             1630..>3924
                     /locus_tag="VIL39_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEY5480424.1"
                     /translation="MGFALLLVFGLSPDAGAAPAQTLNGKHVPAAVARLAPVGSLPGS
                     QRLDLAIGLPLRNQEELDRLLQELCDPTSPNYRRYLTLEQFTARFGPTEKDYQALMDF
                     AKSNGLTVTVTHPNRVVLDVSGTVTAIARTFHLNLRVYPHPREARTFYAPDVEPSVDF
                     AVPILHISGLDNYSLPHPNSRLRPVTAAANTTPNSGTGPSGTYRGSDFRTAYVPGTSM
                     TGTGQSVGLLQFDGFYASDIATYVSQAGLPTIPVTVVPVDGGVSTPGTGNSEVCLDIE
                     MVMSMAPGVSGIYVYEAPNPSPWVDLLSRMANDTNSLGKPLCNQLSCSWGGGGVDASA
                     EQIFKQMGVQGQSFFNATGDSDAFTGAIGFPSDSTNITEVGGTTLTTGTGATYTSETV
                     WNWGLHSGSYVGSSGGISTYYAIPSYQQGISMSANQGSTTQRNVPDVALTADNVYVTY
                     NNGSTGTFGGTSCAAPLWAGFTALVNQQAAAAGLSPVGFLNPVLYTIGKGASYAAAFH
                     DTTTGNNYSSNSPTEFSAVTGYDLCTGWGTPNGTNLINALTAPPILAPIIASNSFVLV
                     SEGCPNGVVDPAETVTVNFGLQNIGTASTTNLVATLLSTDGILSPSGPQTYGVLGAKG
                     TAVVQPFTFTAAGSCGGTNTASLQLQDGAADLGTVTFSFRLGQGGISNVFSQSFDDVT
                     APALPAGWTTATSGSQSAWVTSTSTRDTTPNALFSTDPAKAGVNEVDSPAFTLPDGQA
                     QLMFRNNYNLETSYDGGVLEIKIGS"
BASE COUNT          728 a         1264 c         1214 g          718 t
ORIGIN      
        1 gggacggcat caaagccccg tcgcaattga tcgcagtggg ttgtgaagac tacggcgcgc
       61 tccgtttggc gctcggccag gcgaaccgcc gcgttggcaa aggcgcaaac gccggccgcc
      121 agcggcagtg gctcgcaggc aaagtcccgg gccagccaaa taccgcgcgg ttccaagccg
      181 tgggctttga tccattccgg tggcacccac gggctggtat agaagacttc catcagccag
      241 cctttgcccg caccatctcg aatagcgcct cgacccgcgc ggtgatacgg gcggagtccg
      301 agggagagta atcggtcgtg atcttcaggt agggcaggcg caactcttct tcgacgagct
      361 tgcgtacctg ataggactcg acgtcgtaag tgaggcaggc ctgccagacg agttcgatga
      421 cgcactgagg ccggaacttt gccgccagga cgcgcaggga gtcgaagcgc cgcgggttgg
      481 gcgtcatcac cgagcaggga aggtggtaat acttcttggc gatggcctgg atcgggtccg
      541 gctcgctgtc cagatccacg tcttcgagaa tgggcttaag gccggtgcag ttctccatgc
      601 agacgacgac cgcgccgcag ccttcgatca actcgagcac ccgctcggcg ccatgcgcca
      661 gcgggacgcc ggtcagcagg acgcggaggg gggggcgtga tgcgtgatgc gtgatgcgcg
      721 atgcgtgatt aggcgccttg gcgttgccgc ttaccggcgc atacatctgg agcgcgcaat
      781 cgtattgttg caggtcggcc tcgatgccgg agatggtgct cttgaagtcc agcaattggc
      841 ggccggtgag cggcggcgtt tccagcatca taaggtcggc caaccggcgg cgcaggccgc
      901 gctcgcggtt catcagttgg atggcctgga tgagtctggc ctcagtgata tcggtcgcaa
      961 accgggttgc caggtgctgc tggaacttcc gcacctccag gacccaatgc tccaaggcgt
     1021 cggggtcttc agccttctgc ggcagttcga gcacatacat cggcttggaa gcgcccatca
     1081 gctcgaacat cttcttcttc ccgtcgcagg tggtctccgc cacgatcagg tcagcccact
     1141 tcagaaaggg gttcttgcct gtgacgtggt agccgaaagt ggatttgatc agtggacaga
     1201 gattggccgg caaatactgc tcggcagcgg ggatggtggc cgccgatccg ccgcacaggc
     1261 acacgggcac tccgcccgca gccaggatga gctcgcgcgg ggtgaactcg cacatgatcc
     1321 cgactatcgg ccggccggcg ccccgggcgg cttcggcgta gtcgtagcag tgatcaatca
     1381 tgccggcaaa ccaggccagc gggcctggcg gctcattgcg cgccgcggct aatcgttggc
     1441 aagtatcatc aaccatagtt tcagattccg gcaggatgca tgggggtgcg agcaaactca
     1501 agtcctgccg gcagggcgtc gagtcccata atcgccttgg aaaacgccca aattgtggca
     1561 tgctagcaga agtctatgtt gtaccctcta gtcaagagga actgccagcg ggggcacttc
     1621 ttgcccccaa tgggctttgc tctcttgctg gtgttcggcc tgtcgccgga cgccggggcg
     1681 gcgcccgcac aaaccctgaa cggcaaacac gttccggcgg cggtggcgcg tttggctccc
     1741 gtggggagtc ttcccgggtc gcaacgtttg gatttggcca tcggcctgcc gttgcgcaac
     1801 caggaggaac tcgaccgcct gcttcaggag ctctgcgacc caaccagccc caattaccgc
     1861 cgctatctga cgctcgagca gttcaccgcg cgctttgggc cgaccgaaaa ggactaccag
     1921 gcgctcatgg atttcgcgaa gtccaacggc ttgacggtca ctgtcacgca tccgaaccgc
     1981 gtggtgttgg atgtgtccgg cacggtcacc gccatcgcaa gaaccttcca tctgaacctg
     2041 cgggtttacc cgcatccgcg ggaagcgcgc acgttttacg cgccggatgt cgagccgtcc
     2101 gtggacttcg ccgtccccat cctgcacatc agcggcctgg acaactactc gctcccgcat
     2161 ccgaattcca ggctgaggcc ggtcaccgcc gccgccaata ccacaccaaa ctccggcacc
     2221 gggcctagcg gcacgtatcg cggcagcgac ttccggacag cctacgttcc ggggacttcc
     2281 atgaccggca ctgggcagag cgtcgggctg ttgcagtttg acgggttcta cgccagcgac
     2341 atcgccacct acgtcagcca ggccgggctg cccactattc cggtgacagt tgtgccggtt
     2401 gacggaggcg ttagcactcc cggcaccggg aatagcgagg tttgcctgga catcgagatg
     2461 gtgatgtcca tggcgccagg ggtgtcgggg atctacgtct atgaagcgcc caacccgagc
     2521 ccgtgggttg atctgctcag tcgcatggcc aacgacacga atagcctcgg gaaaccgctg
     2581 tgcaatcagc tcagttgctc gtggggcggc ggcggggttg acgcctcggc tgaacaaatc
     2641 ttcaagcaaa tgggcgttca gggtcagtcc ttttttaatg ccaccggcga ttccgacgcc
     2701 ttcaccgggg ccattgggtt cccatcggac agcaccaata tcaccgaggt cggcggcacg
     2761 acgctgacca ctggcacggg cgccacctac acctctgaaa ccgtttggaa ctggggtctg
     2821 cacagcggca gttacgtcgg cagcagcggc gggatcagca cctactacgc aatcccgtct
     2881 taccagcaag gcatcagcat gtccgccaat cagggctcca cgacccagcg gaacgtcccg
     2941 gatgtggcgc tgacggctga caacgtgtat gtcacgtaca acaacggcag cacggggact
     3001 ttcggaggca ccagttgcgc ggcgccgttg tgggcgggct ttaccgcgct ggtgaaccaa
     3061 caggctgcgg ccgccgggct gtcgccggtt ggcttcctga acccggtcct ctataccatt
     3121 ggcaagggcg cgagctatgc cgccgccttc cacgacacca ccacgggcaa caactatagc
     3181 agcaatagcc caaccgagtt ctccgccgtg accggctacg acctatgcac cggctggggc
     3241 acacccaacg gcaccaacct gatcaatgcc ttaacggccc cgccaattct cgcgcccatc
     3301 attgcttcca acagcttcgt gctggtctcc gagggctgtc ccaacggcgt cgtggatccg
     3361 gccgagacgg tgaccgtgaa cttcggcttg cagaacatcg gcacggccag caccaccaac
     3421 ctcgtcgcca ccctgctgtc cacggacggc atcctctcgc ccagcggccc gcaaacttac
     3481 ggtgtccttg gcgccaaggg cacggccgtg gtccagccct tcacctttac cgctgccgga
     3541 agctgcggcg ggaccaatac cgcctcgctc caattgcagg acggcgcggc ggatctggga
     3601 acggtgacgt tctccttccg gctgggccaa ggcggcatct cgaacgtctt ctcccaaagc
     3661 tttgacgacg tcacggcccc ggccttgcct gccgggtgga ccactgcgac cagcggctcc
     3721 caatcggcct gggtcacctc tacttccacg cgtgatacca cacccaacgc tctcttctca
     3781 accgaccccg caaaggccgg cgtcaacgag gtggattcac cggccttcac gctgccggac
     3841 ggccaggcgc agttgatgtt ccgcaacaat tataatctgg agacgagtta cgatggcggg
     3901 gtgctggaga tcaagattgg gagc
//
LOCUS       DATCGS010000153         5880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_460090, whole genome shotgun sequence.
ACCESSION   DATCGS010000153 DATCGS010000000
VERSION     DATCGS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460090"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..900
                     /locus_tag="VIL39_03005"
     CDS             553..900
                     /locus_tag="VIL39_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480425.1"
                     /translation="MMRIASVSLILALALLRAASAADTIKTEDELKREVANSNSVETP
                     RPQLSANPGAVPEVPMARETNQPAAPPAAPVVVATPEATQSRNVWAIILAVLVLLVGK
                     FALKRLRSRSGPS"
     gene            969..1994
                     /locus_tag="VIL39_03010"
     CDS             969..1994
                     /locus_tag="VIL39_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5480426.1"
                     /translation="MIAAVLHGKENIRVENVVPRPLQAGEVRIQIEAALTCGTDLKVF
                     KRGYHARMLIPPTVFGHEFAGMISELAPDVTGWRVGDRVAVANSAPCGKCFYCLNHQE
                     NLCDDLLFLNGAYAKSIVIPARVVQKNLLRLKPETAFCDAALVEPLACVVQGIEDTQL
                     RSGQRVLVIGAGPIGLMFVALARHLGCQVTVAGRGKKRLETARRLGAELVLNESEQGG
                     LEAAIPPGSIFDVVIEAVGKPETWEAAVRLVRKGGTVNFFGGCPSGTAVSLDTALIHY
                     SNLTLLASFHHTPRSVRRALEFIEAGVVRSDDFVDGECSLTELPMLFRSMASGNRAVK
                     TLVRVRE"
     gene            complement(2062..>5880)
                     /locus_tag="VIL39_03015"
     CDS             complement(2062..>5880)
                     /locus_tag="VIL39_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480427.1"
                     /translation="LKGMTGKWESAICFISAVSAGLLLGLDGFMWKESVVVNRIAVTS
                     VPWFLAVLVCLLRWLYAPHQLRYAYWAAFLFGVCLTLHQSLLVAAIGIEVALAAGNPK
                     LGRDAFLGNFIIYLADYLILAVTGDHMFHNVGAKPGLLFLFNAIGIGSLLTSIWLAIR
                     TKSLGTYWKPVLIMAGLWVLGVSFYLYMAVSGMTNPPMQWGYPRTVEGFFHALSRGQY
                     EQPSPTNPITEPGRFISQVGMLIEGAAAEFTWVYLFIALVPFVFFFKMQKRERAWLIA
                     LTAMYICLGGLLIMLLNPTPDKASADLFKVFMCSSHTIVACLIGYGLAMTAAFMATHY
                     QKFRLWGLAGGIVAVVLALFCLWDATGKHYFGPAGVINLSELPHWIARAFAPNQYGLP
                     IYANLLLVGIAVAFVIALIVYRQRGPLFITLALFAAMPLYSGLAHWFDSDQRNHWFGY
                     WFGHDMFTTKTFKGADGKPLYPDMTKDAVLYGGTDPGRFCPTYTIFCESFTPHRCQPL
                     EDQNFDRRDVYIITQNALADGTYLCYIRAHYNRSTQIDPPFFSELLRTALKDKEYQTN
                     LLARAVAPLDRFFEALGARVEKRRRSYTSWFADNDFTNVVAFAAKLRPNPQQDPLSKY
                     LYENLSPKTQQLLSAQGDQARLRRSLAEDLNVLLERELKIKERLKAKKQEKDAVDQEI
                     ADGNGSDRLRRKQEQLGSELAELSKTGPLYEPERFKSVQLSEYLADFTKENPQSWTRI
                     RLNRLLLEAAYPKEIARSLGGVYPDREIYSATPDDSQRCFQEYMADAQKRMQTGQLKP
                     GEDVKIIDNRVQVSGQVAVMNINGLLTKVIFDHNPKNEFFVEESFPLDWMYPHLTPFG
                     IIMKINRQPLPSLTEDVLARDHQFWKQFSKRLTGDIVDYDTPVKTITDWIEKTYLRHD
                     LNGFTGDRKFLHDDDAQKSFSKLRSSIAGVYAWRLNPDPHVCPPEYRPKSNEEYQRVL
                     KEADFAFRQAFAFCPYSPEAVFRYAQLLLQARRFDDALLVAEACLKLDPYNGQVRGLA
                     DNIRVYKKQSPAIEQAQSTFQHLEEEVKKNPANFQAAFDLAGAYLQMQQTDRAVQVLD
                     GVLNSPQADSKALRGLVTAYSSFSNTPGLQRAADKLGTLARANPDDFQAAIGLAEVSR
                     HLQKPEAANQALDKAFNNPKIDAPSMLSIAQIYAALGNVAKLEAALERLTKVMPESPE
                     AWYDLAVLKCGVGKSPEALSSLRQALDLSAKRFKADPKARDLLASARKEERFSLLRQT
                     PEFKKLVPLQ"
BASE COUNT         1141 a         1716 c         1860 g         1163 t
ORIGIN      
        1 ggaaacgagg gcgacggagt gcgcccgtcc tcgggcgcag caatgttcga acgtaaggaa
       61 gatgcagcag aatcgagcgc ctcgaactat tcggtgcttg ctgcgcccga ggacgggcgc
      121 actccgccaa ggctttgcac gacaaagcct tcctaatgcc acccacaagc cgccttcaag
      181 ccactggcag gccgggagct tgggcatctc tctctattgt tcctctatta ttcctctatt
      241 attcctctat tgttcctcta ttgttcctcg attgttcctc tattgttcct ctattgttcc
      301 tctattgttc ctctagttct gggcctttgg cacagcgggg ctccgatggg agtgctcgcc
      361 ttgccgaggc ctgtggcaat agacggccca cccttggcca ccattcaacc tcgagaccat
      421 cgcggcaaaa aagccagccc caagcctaag cacggcgtgg gcaatgtccg gatgcgcccc
      481 gacatcacag cagcgcaaag aaagggctgt gtcgcatctg gtgcttgggt agtgtatgtc
      541 ttaggcatat acatgatgcg aattgcaagt gtttctctga ttctcgcgtt ggcactgctc
      601 cgagccgcca gcgctgccga tacgataaag accgaggatg agttgaagcg cgaggtggcg
      661 aattcgaact cagtggaaac gcccaggccc caactgtcgg caaaccccgg agcagtgccg
      721 gaggtgccga tggcgaggga gacgaaccag cccgccgctc cacccgcagc cccggtcgtg
      781 gtcgcaacgc ctgaggcgac tcaatcccgg aacgtctggg cgatcatcct ggcggttctg
      841 gtgctgctgg tggggaaatt cgccctaaaa cgtctgcgct cccggtcagg tccgtcttga
      901 gggggtgttg ccccagcaga gacatctctt tctttcgcgg gcgcgcgttg atatactagg
      961 gccacgctat gatagccgct gttctgcacg gcaaagaaaa cattcgggtg gaaaacgtcg
     1021 tgccgcgccc gctgcaagcg ggagaggtgc ggattcaaat cgaggccgcg ctgacctgcg
     1081 gcactgacct gaaagtcttc aagcgcggtt accacgccag gatgctgatt ccccccaccg
     1141 tgttcgggca cgagttcgcg ggcatgatct cggagctggc gccggacgtg acggggtggc
     1201 gcgtggggga ccgcgtggcc gtcgccaact ccgccccctg cgggaaatgc ttctactgcc
     1261 tcaatcacca ggaaaacctg tgcgacgatt tgctgttcct gaacggcgcc tacgccaagt
     1321 ccattgtcat cccggcccgg gtcgtgcaga agaacctgct gcggctcaaa ccggagaccg
     1381 ctttttgcga cgcggcgctg gtcgagcccc tggcttgcgt ggtgcaaggg atcgaggaca
     1441 cgcagttgcg ctccgggcag cgcgtgctgg tcattggcgc cggcccgatt ggcctcatgt
     1501 ttgtcgcgct cgcccggcac ttaggctgcc aggtgacggt ggcgggcagg ggaaagaagc
     1561 ggctcgagac cgcccggcgt ctcggggcag aactggttct gaatgaaagc gagcagggcg
     1621 gtttggaagc tgccatcccg ccgggctcga tctttgatgt ggtcatcgag gccgtcggca
     1681 agccggagac ctgggaagcg gcggtgcggc tcgtgcgaaa gggcggcacg gtgaatttct
     1741 ttggcggttg tccctcgggc acggcggtgt cgctggatac cgcgctcatc cattattcta
     1801 atctcacctt gctcgccagc ttccatcaca cgccgcggtc ggtccggcgc gcccttgagt
     1861 tcattgaggc gggggtggtg cgctctgacg attttgtgga cggcgaatgc tccttaacgg
     1921 agctgcccat gctgttccgg tccatggcct cgggcaaccg ggccgtaaag acactggtgc
     1981 gggtgcgtga atgaagatcc ggctaagcac attctggccc acgcccaatt gattggcacc
     2041 tgccagcgga agtagcgggc gctattgcag cggcaccaac ttcttaaact cgggcgtttg
     2101 tcgcagcaga ctgaagcgct cctcttttct ggcgctggca agcaaatcgc gcgccttggg
     2161 gtcggccttg aaccgcttgg cgctcaagtc taatgcctgc cggagcgagg acagcgcctc
     2221 tggcgatttg ccgacaccgc atttcaacac ggccaggtcg taccaggctt cgggactctc
     2281 aggcatgact ttggtcaacc gctccagagc cgcctcgagc ttggcgacgt ttcccagcgc
     2341 cgcataaatc tgagcaatgc tcagcatgga aggggcatcg atcttcgggt tgttaaatgc
     2401 tttgtccaat gcctgattgg ccgcctcagg tttctgcaaa tgccgggaga cttcggcgag
     2461 gccgatggcc gcctggaagt cgtcggggtt ggcgcgcgcc agggttccga gcttatcagc
     2521 cgcccgctgc aacccggggg tattgctgaa gctggagtag gctgtgacca gcccccgaag
     2581 ggccttcgag tcggcctgcg gagaattcaa caccccgtca agaacctgaa ccgcacggtc
     2641 agtctgctgc atctggagat aggcgccagc gaggtcgaaa gctgcctgga agtttgcagg
     2701 gttctttttc acttcctctt ccaggtgctg gaaggtgctt tgggcctgct cgatcgcggg
     2761 gctctgcttc ttgtaaaccc tgatgttgtc cgcgagcccc cgcacttggc cattgtaagg
     2821 gtccagcttc aggcaggctt cggccacgag gagggcatcg tcgaaccgcc gcgcttgcag
     2881 cagcagttgg gcgtagcgga agacggcttc cgggctgtag ggacaaaacg caaaggcctg
     2941 acggaacgca aaatcggctt ccttgagcac ccgttgatac tcctcattag atttgggacg
     3001 atactccggc gggcagacat gcgggtcggg gttcaggcgc caggcgtaaa cgccggcgat
     3061 ggaactgcgc agtttggaaa aggacttctg ggcgtcgtca tcgtggagga acttgcggtc
     3121 gccggtaaag ccgttgagat cgtggcgcag gtaggtcttc tcgatccaat cggtgatggt
     3181 cttgaccggc gtatcgtagt caacgatgtc accggtcagc cgcttcgaga actgcttcca
     3241 gaactggtgg tcccgagcga ggacgtcctc cgtcagcgac ggcagcggct ggcggttaat
     3301 cttcatgatg atgccgaagg gcgtcaggtg gggatacatc caatccaggg ggaagctttc
     3361 ttcgacgaag aactcgttct tgggattgtg atcgaagatc actttggtca gcaggccgtt
     3421 gatgttcatc acggcgacct ggccggagac ctgcacccgg ttgtcgatga tcttcacatc
     3481 ctcacccggc ttcaactggc ccgtctgcat ccgcttctgg gcatcagcca tgtattcctg
     3541 gaagcatcgc tgcgaatcgt caggcgtggc cgagtagatt tcgcggtccg ggtaaacccc
     3601 gcccaggctg cgggcgattt ccttcgggta agcggcttcc agcagcaggc ggttcagccg
     3661 gatgcgggtc caactctgcg ggttctcctt ggtgaaatcg gccaggtatt ccgaaagctg
     3721 cactgacttg aaacgctccg gttcgtacaa cgggccggtc ttggacaact cggcgagctc
     3781 ggaaccgagc tgctcctgct tccggcgcag acggtcagac ccgttgccat cggcgatttc
     3841 ctggtccacc gcgtccttct cctgcttctt ggctttgagg cgttccttaa tcttgagctc
     3901 gcgttcgagc agcacgttca ggtcttcggc caggctgcgg cgcaggcggg cctggtcgcc
     3961 ctgggctgaa agaagctgtt gggtcttagg gctcagattc tcatagaggt atttcgagag
     4021 cgggtcctgt tgcgggttgg gacgcagctt ggccgcaaag gcgaccacgt tggtgaagtc
     4081 attatcggca aaccacgagg tgtaagagcg ccggcgcttt tccacccggg cgcccagcgc
     4141 ctcaaagaac cggtccagcg gcgccaccgc ccgggcgagc aggttcgtct ggtactcctt
     4201 gtccttaagc gccgtccgga gcagctcgct gaagaacggc ggatcaatct gcgtgctgcg
     4261 gttgtagtgg gcgcggatgt aacacaggta ggtgccgtcg gccagggcat tttgggtgat
     4321 gatatagacg tcacggcggt caaagttctg gtcctctagc ggctggcagc ggtgcggcgt
     4381 gaaactctcg cagaagatgg tgtaggtcgg gcagaagcgg ccggggtcgg tgccgccgta
     4441 gagcaccgcg tcttttgtca tatccggata caacggcttg ccgtccgccc ccttgaacgt
     4501 cttggtggtg aacatatcat ggccgaacca gtagccgaac cagtggttgc gctggtcgct
     4561 gtcgaaccag tgggccaggc cggagtaaag cggcatggcg gcaaagaggg ccagcgtaat
     4621 gaacagcggg ccgcgctggc gatagactat cagcgcgata acgaaagcca ccgcgatgcc
     4681 caccagcagg aggttggcat agataggcag gccgtattga ttcggggcaa aggcccgcgc
     4741 aatccaatgc ggcaactcag agaggttgat cacgccggcg ggaccaaagt agtgcttgcc
     4801 ggttgcatcc cagaggcaaa acaaggccag cacgaccgcg acgatacccc ccgccaaacc
     4861 ccaaagccga aatttctggt aatgcgtggc catgaaggca gccgtcatgg ccaggccgta
     4921 cccgatcagg cacgcaacga tcgtgtggga agagcacatg aagaccttga agaggtcggc
     4981 cgaggctttg tcgggcgtgg gattgagcag cattatcaac aggccgccca ggcagatata
     5041 catggcggta agggcaatga gccaggcgcg ttcgcgcttc tgcatcttga aaaagaacac
     5101 aaaaggcacc agcgcaataa acaggtaaac ccaggtgaac tcggcagccg caccttcgat
     5161 cagcatgccc acttggctga tgaaccggcc cggctcggtg atcgggttgg tcgggctggg
     5221 ctgttcgtac tggccgcggc tgagcgcatg gaagaaccct tccaccgtcc gggggtagcc
     5281 ccactgcatc ggcgggttgg tcatgcccga aaccgccatg tagaggtaga acgaaacccc
     5341 gagcacccag agcccggcca tgatcagcac tggcttccag taggttccca aactcttggt
     5401 ccggatggcc agccagatgc tggttagcag cgaaccgatc ccgatggcgt tgaagaggaa
     5461 taacaatccg ggcttggccc cgacgttgtg gaacatgtgg tcgccggtta ccgccagaat
     5521 cagatagtcc gccaaataaa tgataaagtt gcccaggaaa gcgtcccggc ccagcttcgg
     5581 gttgccggcg gcaagcgcga cctcgatgcc tatggcggcg acgagcaggc tttgatgtaa
     5641 cgtgaggcaa accccgaaca ggaaagccgc ccagtaggcg taacgcaact ggtggggcgc
     5701 gtagagccac cgcagcaggc agaccagcac ggccagaaac caaggcacgc tggtgacggc
     5761 gatgcggttg acgaccaccg actccttcca catgaacccg tcaaggccca gcagcaggcc
     5821 cgcggacacc gccgagatga agcagatggc gctttcccat ttgccggtca tccccttgag
//
LOCUS       DATCGS010000154         4160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_261696, whole genome shotgun sequence.
ACCESSION   DATCGS010000154 DATCGS010000000
VERSION     DATCGS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261696"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="VIL39_03020"
     CDS             complement(<1..1804)
                     /locus_tag="VIL39_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5480428.1"
                     /translation="MKRLLFLALALCVLVPQLQLSAQTAQTNEEQQLIGVLQSSQSPR
                     EKDAACARLKRIGTAQSVPALAALLTDEQLSHSARYALESMPSAKAGQVLTDALGKTS
                     ALTKVGIINSLGFRHETQAVPALATLLTDQDSHVAAAAATALGQIGGSKALTALQAAT
                     SNSAGPVHDALVDACLRCANSLLAAGSQSKALAAFQQLYDTEKKDGIRTAAYRGMIQA
                     SGKRALPLMTTAILGADGASQIAALQLVREVTAPGATAAFATLLHTVDPPVQAALIGG
                     LSQRGDVLAVPAIAAMVSSNAPEVRLAAINALGILGDASFVPLLAVSAASASGEEQKA
                     ARLALVQLWRGNPTETLVRLMPAAKPEVQAEFARALGDRSDKAAVPKLIELAREGAGS
                     ASKAALQALVLLVDDAQVGQMVQFVIDAKTETARAAAAEALNSACHQIQTRRGQVNIE
                     PLVQGLATAPTDARIAILPVCSGLIDPKVRSALRAAIAASDPQVRAAAIRALCDTSDA
                     ELLPDVVKIACGAPEANFRTLAIRACVRLTTQEETIKLPIKEQIEPLKTILATTLSAD
                     QKCVVLAGLAEIPHSEALKLAEPMLDEAAIQLEAA"
     gene            complement(1824..3137)
                     /locus_tag="VIL39_03025"
     CDS             complement(1824..3137)
                     /locus_tag="VIL39_03025"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480429.1"
                     /translation="MNSAHRVTRRQFLHQTTTALGAALAVPAIVPASVLGRGGAMPPS
                     ERITMGFIGIGGQGGGHLLGGAWTYVAGGYAGRKDVQVLAVCDVWRERRENACKKVND
                     HYAEAYGKGNYKSCEAYTDFRQVLDRPDIDAVLIATPAHWHATMAAMAAAAGKDIYCE
                     KPSAVTVRESQAMLAAVRRYGRVYQAGTQQRSEYDGKFRRACEFVRSGRIGKLQAIYA
                     YRDGGGVFWPQRFGPAKPVPAGLDWDLYLGPAPWLPYDGNTGAHRFDIGELNWGQHHY
                     DIVQWAAAADDTGPVELFMEKDRSCYKYASGVVVYGKPYPGEPVGGDGGACFVGTAGR
                     IAVDRDALVSDPPDIVREPLRPNEVHLYRTTSHAGNFLECIRTRKRPICDANIAHRAA
                     SALLLGGVVKQLKRPLKWDPQAEQFVKDDEANRMLSIAKRPPWCI"
     gene            3282..4094
                     /locus_tag="VIL39_03030"
     CDS             3282..4094
                     /locus_tag="VIL39_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5480430.1"
                     /translation="MKIMLLSAVALLALSLSMPSGVAADPSQASTDLKALVTKIQTKL
                     RDGKKTEADLADEIKEFDALLAKHKDEKTDDVAQILLMKASLYEQVFKDTAKGDALKA
                     QLKRDFPDSEPVKMMKQQEEATKLKAALAEGTKFPDFNEKDLDGKPLSIANYKGKVVL
                     LDFWATWCGPCVRELPNVIKTYDAYHTKGFEIIGISLDKDREKLVSFTNEKKMTWVQY
                     FDGLFWKNKLAVKYGVNSIPATYLLDGQGTIIGKDLRGEALDEAVAKALAKK"
BASE COUNT          768 a         1276 c         1369 g          747 t
ORIGIN      
        1 tcgcggcctc aagctggatt gcggcctcgt ccagcatcgg ttcagccagt ttgagcgctt
       61 ccgaatgcgg aatctcggcc aggccggcga gaaccacgca cttttgatcg gcgctgagcg
      121 tggtggccag gatcgtcttc aggggctcga tctgctcctt gatggggagc ttgatagtct
      181 cctcctgcgt cgtcagccgc acacaggcac ggatggccag ggtgcggaag ttagcctccg
      241 gcgccccgca ggcgatcttc accacatccg gcaacagttc agcgtcgctc gtgtcgcaca
      301 gcgcgcggat agcggcggcg cggacctgtg ggtccgaggc tgcgatcgca gcgcgcaggg
      361 cggagcgcac cttgggatca atcagcccgc tgcaaacagg gaggatggca atgcgggcgt
      421 cagttggggc ggtcgccaat ccttgcacca gcggttcgat gttcacctgg ccgcgccggg
      481 tctggatctg gtggcaagcg gagttaaggg cttcggcggc ggcggcgcgc gcggtctcgg
      541 tcttggcgtc aatgacaaac tgcaccatct ggcccacctg ggcatcatcc accagcagga
      601 ccagagcttg gagcgcggct ttgctggcgg agccggcccc ttcccgtgcc agttcgatca
      661 gcttcggcac ggcggccttg tcactgcgat cgccgagggc gcgcgcgaac tcggcctgca
      721 cttccggctt ggcggcgggc atgagccgca ccagtgtttc ggtgggattg ccccgccaca
      781 attggaccag tgccagccgg gctgccttct gttcctcgcc gctcgcggaa gcagcggaca
      841 ctgccagcaa cggaacgaac gaagcatcgc ccaaaatgcc gagggcgttg atcgcggcca
      901 agcgcacctc cggcgcgttg ctgctcacca tcgcggcaat ggctggcaca gccaaaacat
      961 ctccgcgctg actcaaaccc ccgatcagag ccgcctgcac ggggggatca accgtatgca
     1021 gcagcgtcgc aaaggccgct gttgcgccgg gagcggtcac ctcgcgcacc aactgaagcg
     1081 ctgctatctg gctggccccg tccgctccca ggatggcagt ggtcatcagg ggcagcgccc
     1141 gtttgcccga ggcctggatc atcccgcgat aggcggctgt gcggatgccg tccttcttct
     1201 cggtgtcata gagttgttgg aaggctgcga gcgccttcga ctgactgccg gctgcgagca
     1261 ggctattggc acagcgcaaa caggcatcca cgagggcgtc gtggaccggc ccggcggagt
     1321 tcgaggtggc ggcctgcaag gctgtgaggg ccttggagcc gccaatctgg cccagggctg
     1381 tggcagccgc cgccgccacg tgggagtcct gatccgtcaa cagcgtggcc aaggcgggca
     1441 cggcctgcgt ctcgtgccgg aacccaagag agttgatgat gccgaccttc gtcagcgcag
     1501 aggtcttgcc cagggcgtcg gtcaggacct ggccggcttt ggctgagggc atggattcca
     1561 gcgcataacg ggcggagtgg gagagctgtt catccgtaag cagcgccgcc agtgccggca
     1621 cggattgggc ggtgccgatg cgcttaagcc gggcgcaggc ggcatccttt tccctgggtg
     1681 actggctgga ctggaggaca ccaatgagtt gttgttcctc gttggtctgc gcggtttgcg
     1741 ctgagagttg aagctgcggc acaaggacac acagcgccaa ggctaagaag agtaatcgtt
     1801 tcatgggaga tcctttctga gtatcaaatg caccacggtg ggcgtttggc gatggagagc
     1861 atccgattgg cctcgtcatc cttgacgaat tgctcagcct gcggatccca cttgagcggg
     1921 cgcttcaact gtttgacgac accccccagc agcagcgcgc tggctgcccg gtgggcaatg
     1981 ttagcgtcgc agatgggccg tttgcgggtg cggatgcact cgaggaagtt gccggcgtgg
     2041 ctggtggtgc ggtagaggtg aacctcattc ggtcgcagcg gttcgcgcac gatgtccggc
     2101 gggtcggaca ccagcgcatc gcgatccacc gcgatgcggc ctgccgtgcc gacgaagcaa
     2161 gcaccaccgt cgccgcctac aggttcgccg gggtagggct tgccatacac gaccacgcca
     2221 ctggcatact tgtagcagga acggtccttc tccatgaaca gttcaaccgg ccccgtgtcg
     2281 tctgccgccg ccgcccattg gacgatgtca tagtggtgct gcccccagtt gagctcgccg
     2341 atgtcaaagc ggtgagcgcc ggtattgcca tcatagggaa gccagggcgc gggccccagg
     2401 tagaggtccc agtccaggcc ggcggggact ggcttggcgg ggccaaaacg ttgcggccag
     2461 aaaacgcctc ccccatcgcg gtaggcgtag atggcttgca gtttgccaat gcgcccgctg
     2521 cggacgaatt cgcaggcgcg gcgaaacttg ccgtcgtatt cactgcgctg ctgggtgccg
     2581 gcctgataga cccgcccgta acgccgcacc gccgccagca tagcctggct ctcgcgcacg
     2641 gtgacggcgc tcggtttctc gcaatagatg tctttgccgg cggcggcggc catggcggcc
     2701 atggtggcat gccagtgggc cggggtggcg atgagcacgg cgtcaatatc cgggcggtcc
     2761 agcacttggc ggaaatcggt gtaggcttcg caggacttgt agttgccctt gccgtaagct
     2821 tcggcgtagt ggtcattgac cttcttgcaa gcattctcgc ggcgctcgcg ccaaacgtcg
     2881 cacaccgcca gcacctgaac atccttgcgc ccggcatagc cgccggcgac ataggtccag
     2941 gcgccgccca gcaggtggcc gcctccctgg ccgccaatgc caataaaccc catcgtgatg
     3001 cgctcgctgg gcggcatggc tccgccccgg cccaaaaccg aggcgggaac aatggccggc
     3061 acggccaagg cagcgccaag ggcggtggtc gtctggtgca ggaactgacg gcgggtaact
     3121 ctgtgtgctg agttcataag ctgattctgt gcgactgcgc ggacggcagg ttatggcgtt
     3181 ggccgcagct tgtccagcga ttgcgccgct gacatgtctt tttctcttgg cgggtgaaac
     3241 tccccttgcc gtgggcgggg taattaacta ttctcgcgcc aatgaaaatc atgcttttgt
     3301 ccgcagtcgc cctcctggcg ctctcactat ccatgccgag cggggtcgcc gcggatccat
     3361 cccaggcctc caccgacctc aaggcgcttg tgaccaaaat ccaaaccaag ctgcgggacg
     3421 gcaagaagac cgaagcggac ttggcggatg agatcaagga gttcgacgct ttgctcgcca
     3481 agcataagga cgagaagacc gacgacgtag cgcagatcct gcttatgaaa gcctcgctct
     3541 acgagcaggt tttcaaggat acggctaaag gcgacgcgct gaaagcgcag ctcaagcgag
     3601 actttccgga ctccgagcct gtcaagatga tgaagcagca ggaagaagcg actaagctca
     3661 aagcggcgct ggcggaaggc accaagttcc ccgatttcaa tgagaaagac ctggacggca
     3721 agcccctctc catcgccaat tacaagggca aagtggtgct gctcgacttc tgggcgacct
     3781 ggtgcgggcc gtgcgtccgt gaactgccca acgtgatcaa gacgtatgac gcgtaccaca
     3841 cgaagggctt tgagatcatt ggcatcagcc tggacaaaga ccgggagaag ctcgtcagct
     3901 tcaccaatga gaagaagatg acgtgggtgc agtatttcga cgggctgttc tggaagaaca
     3961 agctggccgt caagtatggc gtcaacagca tcccggcgac ctacctgctc gacgggcaag
     4021 gcaccatcat cggcaaagac ctgcgcggag aagcactcga cgaggcggtg gccaaagcgc
     4081 tggccaagaa gtaggcccga ctggctgaag ccagagccgg gcccgatgtc ctgaccgcaa
     4141 gcgtctagct ctttgcggcg
//
LOCUS       DATCGS010000155         9969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_707792, whole genome shotgun sequence.
ACCESSION   DATCGS010000155 DATCGS010000000
VERSION     DATCGS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707792"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1235
                     /locus_tag="VIL39_03035"
     CDS             279..1235
                     /locus_tag="VIL39_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480431.1"
                     /translation="MSNNNFSAQSIALKQRLLLPLVATFFILALTPRCVAATAIYSTG
                     FEASEGYNINLDLVGQNGWMSAGSGGNGLLTDVFPGRGQQAYIGYAPPASGDSSLFIY
                     QPINKALAHVQFSVTVSVFDSTTTDWDDFYWGVYNQEGDALFSLDFDNYDLRIYYYLD
                     GTNSRVWSGLEFTNGVAYPLRVDLDFTSNRWSATFNGALLATNEPITTIGSPLNLGDI
                     DPAWIIFDPNAPGDNYMVFDDYLITASLPPPQLRLLGMLGSAPVLRLSGSPDTAFAIE
                     ASTNLVRWVPLKTNVTTGGSFDYVDNGAAGLPRRFYRGRWVP"
     gene            complement(1307..1735)
                     /locus_tag="VIL39_03040"
     CDS             complement(1307..1735)
                     /locus_tag="VIL39_03040"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEY5480432.1"
                     /translation="MSAVNLVAVFVENKPGQTARVTKLLADVGVSLCWLTIANSGSFG
                     VMKFLVDQRDLAVQTLKEKGLMVSLLEVLAVEVPHKPGSLQAVADLLGRNDINLDNCS
                     GFVANNRAILVVEVQELAQARAIFEKQGLRLLTQEEMLRL"
     gene            complement(1778..3070)
                     /locus_tag="VIL39_03045"
     CDS             complement(1778..3070)
                     /locus_tag="VIL39_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY5480433.1"
                     /translation="MFWDKESETLTRPTLEQLQLKRLQNTVECVAQRVPFYRDKFTKL
                     GIAPADIKSLADVRRLPFTTGADLRTIYPEGMLAVDRDEPVRLHTSSGTTGKPKAIFF
                     SRKDVENAAELVARSLVMTGITRKDVLQNMMSYGLFTGGLVMHYGAEKVGCLVIPSGP
                     GTSERQLMLMQDFRSTAVHILPSYALYFASFLEHKGIDPRKDLSVRKAFVGAEPHTEE
                     TRRRIEQIFGCDVYNSYGLTEMNGPGTAFECEHKAGMHLWEDNFILEIINPATGEPLP
                     EGQTGELVMTTLNREAMPILRYRTRDITSVIPGPCKCGRTHRRIARFTGRSDDMLIIR
                     GVNIFPQQIERVLMSFPQVGRNYVIIIEGLDDLTVKVELAAAGFDGQVEHVAALQKQL
                     VEKLKAETYVRPKVDLLPAGSLPVAEGKAKRVIDKRSL"
     gene            3616..5244
                     /locus_tag="VIL39_03050"
     CDS             3616..5244
                     /locus_tag="VIL39_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5480434.1"
                     /translation="MNESNRLLLSGDEAIALAARHAGVALGAGYPGTPSTEILERFSE
                     LGGHAQWSPNEKVALEVAIGAAFGGARVLCTMKHVGLNVAADPLFTAAYTGVSGALVI
                     VSADDPGMASSQNEQDNRRYAVAAGVPMLEPSDSQEAYDFMWAAIEISERWKIPVLYR
                     ATTRVCHSYTVVQPRENHSAPKPANFERDIRGHVMIPAYARPAHRRLRHKLAEMQEWN
                     ETCSLNRIVQGGQSLGIITSGVSFMHVREAAPEARVLKLGFTHPLPMKKIAQFVKGVG
                     RCVVIEEGDPYLVEAIRAASIPVEGKADMYRFGELDVTRVQRILKNDLSPEPVKPPGK
                     PPQLCDACQYRIVFESLRKKDCIVAGDIGCYTLGVLPPFEAIDSCVCMGASLGVGLGL
                     RHVLPPDQARRVVSIIGDSTFVHSGISGLVEMVYNPPPTGHVLIILDNSTTAMTGHQE
                     HPGTGRKLNHEPTGKVVLEDLARSLGIRRVHVIEPHAGTGEFDRLLTDCLESNELAVI
                     IARRPCILIAKQLKQYEAQQYECNAGKAAAPADS"
     gene            5268..5753
                     /locus_tag="VIL39_03055"
     CDS             5268..5753
                     /locus_tag="VIL39_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEY5480435.1"
                     /translation="MNKVTNIVVAGLGGQGVLKGTDILADVALRAGYDVKKSEIKGMS
                     QRGGSVTGEVRFGEQVFSPMVPAGEADFLLVLEPTQVEPHRYMLRPGGILITPDAVRA
                     DQLPSKKTLNVALLGALSAHLPMVEEQWLTSLRSGFAESFFDGNQKAFLIGRDSQKPA
                     K"
     gene            5769..6167
                     /locus_tag="VIL39_03060"
     CDS             5769..6167
                     /locus_tag="VIL39_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY5480436.1"
                     /translation="MDTVKRFFKNDKFAERANIELLSVSPGQARAKMTLHRHHMNGYG
                     TVQGGAIFTLADFAFAAASNSHGKVAVAINVSITFMKAGESGTLWAEAREVSKNIKLG
                     SYTVEVKDDQGELVAVFQGLAYRKSEAIPV"
     gene            complement(6184..6624)
                     /locus_tag="VIL39_03065"
     CDS             complement(6184..6624)
                     /locus_tag="VIL39_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEY5480437.1"
                     /translation="MKVKQLSLFLENKLGSLSRPVKLLAKAKLNILTLSIADTRQFGI
                     LRFVVRDWEKAKQVLEEDGFIVKVSDLVAIEVADEPGGLAEILVRVEKARVNLEYMYG
                     FTLKQEGKGVLAFRFDDPDRAIAALQKKGISPIRSVDLFKRLES"
     gene            complement(6643..7974)
                     /locus_tag="VIL39_03070"
     CDS             complement(6643..7974)
                     /locus_tag="VIL39_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY5480438.1"
                     /translation="MSKHAKSSHRSFHPLSAPDFLPLPQLRDLQLQRLTAIVQRAYDR
                     VALFRKRMDERGLTPLDVRTLDDIVHLPFAEKTDLRDTYPFGLFASPMKEIVRLHVSS
                     GTTGKPIVVAYTQEDIQVWTNVMLRSFAACGLHEGDVIQNAYGYGLFTGGLGAHYGAE
                     ALGATVIPISGGNTPRQLMVMKDFGVTAICCTPSYFLHLIDQAVEAGVKFKELPLRVG
                     VFGAEPWTESMRRRIEADSGIKAYDIYGLSEIIGPGVAMECQCQAGPHIFEDYFYPEI
                     INLKTGKPCPDEEEGELVLTTLGKKAMPMIRYRTRDITALTSETCECGRTLRRIKRIG
                     RRADDMFIIRGVNVFPSQIETALLKVEGTLPHYQIVLTRERDLDVMEVQVEVTSEFFT
                     DTVGALEQLQSQLSKSIETTVGVRANVRLVQPHTIQRSEGKAKRVIDQRKM"
     gene            8107..8754
                     /locus_tag="VIL39_03075"
     CDS             8107..8754
                     /locus_tag="VIL39_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5480439.1"
                     /translation="MDPRILIDGLILYLGLLVLLTFHEFGHAWMAWKCGDDTALSQGR
                     VSLNPLVHIDLIGTVAVPLLLIFFAPGGFFVGWAKPVPVEPNNLRNPRLDDIMVTLAG
                     PLMNLLLAIVILALARFGYAVHSRGLVEVCFNLAHLSLLLCFFNLLPIPPLDGSQVAR
                     SLIGMSYETYHQIARYGIFILIVVLQVPGVQRTLDVLTTNSWVSIAGWFGMPRLI"
     gene            8816..>9969
                     /gene="xylB"
                     /locus_tag="VIL39_03080"
     CDS             8816..>9969
                     /gene="xylB"
                     /locus_tag="VIL39_03080"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY5480440.1"
                     /translation="MRTLLLGIDSGTQSTKVLVVDARDGKAIASAAQEYELIPNLPPG
                     AKEQHPHTWREATASAMRRALRQAKAVAAEVKAIGVSGQQHGFVPLDKAGEVIRPAKL
                     WCDTSTAAECEEITEQLGGLKQTIRALGNAVLPGFTASKILWLKKHEPKNYQRLATVL
                     LPHDYLNFWLTGEKTMEYGDASGTALLDVRKRKWSEAVLNAIDPDLAGKLPPLMRSDR
                     PAGLLQASTAKQLDLNPGVLVSAGGGDNMMGAIGTGNTRAGVITASFGTSGTIYACSE
                     KPVVDPQGEIAAFCDSTNRWLPLLCTMNVTVATEMVREDFGWSHEKLGVEAARIPAGS
                     RGLLLLPYFEGERTPNVPDGTGVWFGVNQKTFEAGHFARAAMEGVTLGMN"
BASE COUNT         1901 a         2959 c         3123 g         1986 t
ORIGIN      
        1 gaaagcaagt ggtgttcgag atggatccgc gccctcgcgt cgcgccgaag ctggaagcgt
       61 tcccccgctg aattggcatc acggcccgct gggccgctgg aatcaggttg gcaggtcgta
      121 ttggcatggc agaccgttcc aaccaaatct caagacggtc aggggggact attcgtatca
      181 atgaacccta tcacttccgt aaagaggccc ggcgatttcg cggctcactg ttccaccttg
      241 acatctgtga gaggcggggt acggtcgtca taataagcat gtccaacaac aacttctctg
      301 ctcaaagcat agctttgaaa cagcgattgc tcctcccatt agttgccacc ttcttcattt
      361 tggctttgac cccgcgctgt gtggcggcaa cagccatcta ctccacaggc tttgaagcca
      421 gcgagggcta caacatcaac ctggacctgg taggccaaaa cggttggatg agcgcaggct
      481 ccggcggaaa cggcctgtta acggacgtgt ttccaggacg aggccagcag gcatacatcg
      541 gttacgcgcc gccagcctcg ggcgactcca gccttttcat ttatcagccg atcaataagg
      601 ccctcgcgca tgtgcagttt tcggtgacgg tgagcgtgtt tgactccacc acaacagact
      661 gggatgactt ttactggggc gtgtataatc aagagggcga tgcgcttttt agcctcgatt
      721 tcgataatta cgacctcagg atttactatt acctggatgg caccaactcc agagtgtggt
      781 ctggactcga gttcacgaac ggcgtcgcat acccattgcg cgtggatctt gatttcacga
      841 gtaaccgctg gagcgcgaca ttcaatggtg cgctgctggc gaccaacgag ccaatcacca
      901 ccattggttc accgctgaac ctgggtgaca ttgatcccgc gtggatcatt ttcgacccaa
      961 atgctcccgg tgacaattac atggtgttcg acgactacct tattaccgct tcgctaccgc
     1021 cgccgcagct taggctgctt ggaatgctag gcagcgcgcc ggtgctgcgc ctgtccggta
     1081 gcccggacac ggcattcgcg attgaggcct ccaccaatct tgtgcgctgg gtgcccttga
     1141 agactaacgt tacaacaggc ggctcctttg actacgtgga caatggagcg gctggcctgc
     1201 cgcgccggtt ctaccgtggg cggtgggtgc cttagcgccc aagcggctgg ctgcccggaa
     1261 gggagtcttc ggcgggcaaa cagcttggac atgggaatgg tccgctctat agccggagca
     1321 tttcctcctg ggtgaggagg cgcaggccct gcttttcgaa gatggcgcgg gcttgggcga
     1381 gttcctggac ttcaaccacc aggatggcgc ggttattggc cacaaagccc gaacagttgt
     1441 ccaggttgat atcgttgcgg cccagcaggt ccgccaccgc ttgcaatgag ccaggcttgt
     1501 ggggcacttc cacggccagc acttccagca gcgaaaccat tagacccttc tctttcaggg
     1561 tctgaacggc caaatcgcgc tggtccacca ggaatttcat tacgccgaag ctgccgctgt
     1621 tggcaatggt gagccagcag aggctgacgc cgacgtcggc cagcaatttg gtgacgcggg
     1681 cggtctggcc tggtttgttc tcgacgaata cggctacgag gttgacggcg ctcatggggc
     1741 aatattggat tgatggattg gtggattgtt gaggggctta gagggagcgt ttgtctatga
     1801 cgcgtttggc tttgccttcg gccaccggca aggaaccggc cggcaggagg tccaccttgg
     1861 gcctgacata ggtttcggcc ttgagtttct cgaccagttg tttttggagg gcagccacgt
     1921 gctcgacctg cccgtcaaaa ccggcggcgg ctagctcgac tttgacggtt agatcgtcca
     1981 ggccctcaat gatgatgacg tagttgcggc caacctgcgg gaaggacatc agcacgcgct
     2041 cgatttgctg cgggaagatg tttacgccgc ggatgatgag catgtcgtcg gagcggccag
     2101 tgaagcgagc tatacggcga tgggtgcggc cgcatttgca gggtccagga atgacggagg
     2161 tgatgtcccg ggtgcggtag cggaggatgg gcatggcttc gcggttgagg gtggtcatca
     2221 ccagctcgcc ggtctggcct tcgggcaaag gctcgccggt ggccgggttg atgatctcga
     2281 ggatgaaatt gtcctcccag aggtgcatgc cggctttgtg ctcgcactcg aaggcggtgc
     2341 cggggccgtt catctcggtg aggccgtaag agttatagac gtcgcagccg aagatctgct
     2401 cgatgcgacg gcgggtttcc tcggtgtgcg gctcggcgcc gacaaaggcc ttgcgcacgg
     2461 aaaggtcctt gcgcgggtca atgcccttgt gctcgaggaa gctggcgaag tagagggcgt
     2521 agctgggcag gatgtgaacc gcggtgctgc ggaagtcctg catgagcatc aactggcgct
     2581 cggaagtgcc cgggccggag ggaatgacca ggcaaccgac cttctccgcg ccgtagtgca
     2641 ttaccagccc gccggtgaag aggccatagc tcatcatgtt ctgcagcacg tccttcctgg
     2701 tgatgccggt catgaccagc gagcgggcaa cgagttcggc ggcgttctcc acatccttgc
     2761 gcgagaagaa gatggccttg ggtttgccgg tggtgccgct cgaagtgtgc aggcgcaccg
     2821 gctcgtcgcg atccaccgcc agcatgccct caggatagat ggtccgcagg tcggcgccag
     2881 tggtgaaagg cagccgccgc acgtcggcca gcgacttgat atccgccggg gctatgccca
     2941 gcttcgtgaa cttgtcccgg taaaagggca cgcgttgtgc cacacactcg acggtgttct
     3001 gcaaacgctt gagctgcagt tgttcgagtg ttggacgggt cagtgtctca gattctttat
     3061 cccagaacat gatggtgaag cgattgtatg ccgggagtcg tgagggttgt ccatcccaat
     3121 ccacagccgc gtgtgaccgg aggcgcatct cggcatcgcc acaggagcgc ggacattcct
     3181 gtccgcagca agctccgaaa agcaacgtgc tctggctagt tcccaaacca ttttacccat
     3241 cgaacgttgc tgcggacaag aatgtccgcg ctccgacccg gtggcagtgc caagaaacac
     3301 cctgtgaccg gaggacgcgc gcgtccttgc caccccgctt ccgcgacgct gacctcgggc
     3361 ctctggcagg cttgatgcca cggtactgcc cgcgtgttgc tagcctgttg cccgcgtgat
     3421 gcttcgaagg agttacgatg gggttacgat gggattcaag ggaaatcagc tccccataac
     3481 ggtccctcga ccgtctcgac aacgccaagc caaggtggca ttccgcagtc actcgccctg
     3541 gcaacgggat gcgcaatcga ttttgttttt caaaagccaa gagaaagggt attaggctgc
     3601 cgggtattat attgcatgaa tgagtcaaat agactgctgt tgagcggcga cgaagccatc
     3661 gcactggcgg cgcggcacgc gggcgtcgcg ctcggggccg gctacccagg cacaccgtcc
     3721 acggaaatcc ttgagcgttt ttctgaactc ggtgggcatg cccagtggtc cccgaacgaa
     3781 aaggtggcgc tggaagtggc catcggcgcg gccttcggcg gggcgcgagt gctgtgcacc
     3841 atgaaacacg tcggcctgaa cgtggctgcc gacccgctct tcacggcggc ttataccggg
     3901 gtgagcggcg cgctggtcat cgtctccgcc gatgacccgg gcatggcttc gagccagaac
     3961 gaacaggata accggcgtta cgccgttgcc gccggggtgc caatgctcga gccgtccgac
     4021 tcgcaggagg cgtatgattt catgtgggcg gcgatcgaga tatcggagcg ctggaagatt
     4081 ccggtgttgt accgcgcgac gacgcgcgta tgccacagct ataccgtcgt gcagccgcgc
     4141 gagaaccaca gcgcacccaa accggcgaac ttcgagcggg acatccgggg gcacgtgatg
     4201 atcccagcct atgctcgtcc ggcccaccgg cgcctgcggc ataaactggc cgagatgcag
     4261 gaatggaatg agacctgttc tttgaatcgc atcgtgcagg gcggccaatc gctcggcatc
     4321 atcacttcgg gtgtttcctt catgcacgtc cgggaagccg cgccggaggc gcgcgtgctc
     4381 aagctggggt ttactcatcc gctgccgatg aagaagatcg cgcagtttgt gaagggcgtc
     4441 gggcgctgtg tggtcatcga ggaaggcgat ccatacctgg tcgaagccat ccgcgccgcg
     4501 agcatcccag tggaaggcaa ggccgacatg taccggttcg gcgaactgga cgtgacccgc
     4561 gtgcagcgca tcctcaaaaa cgacctcagc ccggagccgg tcaaaccgcc gggcaaaccg
     4621 ccgcagctct gtgacgcatg ccaataccgc atcgtcttcg agtcgctgcg caagaaggat
     4681 tgcatcgtgg ccggcgacat cgggtgctac acgctgggcg tgctgccgcc gttcgaggcc
     4741 attgactcct gcgtttgcat gggggccagc ctgggcgtcg ggctgggcct gcgccacgtg
     4801 ctcccgcctg accaggcccg ccgcgtggta agtatcatcg gcgacagcac cttcgtgcat
     4861 agcggtatca gcgggctggt ggagatggtt tataatccgc cgccgaccgg ccatgtgctg
     4921 atcatcctcg acaattccac aacggcgatg accgggcatc aggagcaccc aggcacaggc
     4981 cgcaagctga accacgaacc gaccgggaag gtggtgctcg aagacctggc ccggtcgctg
     5041 ggcatccggc gggtgcatgt catcgaaccg cacgccggca cgggcgagtt tgaccgcctg
     5101 ctgacggact gcctggaaag caatgaactg gcggtgatca tcgcccggcg gccgtgcatc
     5161 ctcatcgcca aacaactcaa gcaatacgaa gcacagcagt acgagtgcaa cgccggcaag
     5221 gctgccgccc ccgccgactc ctgatcacgc atcacgccat tcccctcatg aacaaagtca
     5281 ccaacatcgt tgtcgccggc ctcggcggcc agggcgttct caaaggcacc gacattctgg
     5341 ccgacgtcgc gttgcgcgcc ggttatgatg tgaagaagag cgagatcaag ggcatgagcc
     5401 agcgcggcgg ctcagtgacg ggcgaagtgc ggttcggcga acaggtcttc agcccaatgg
     5461 tgccggctgg cgaagcggat ttcctcctgg tgctggagcc gacgcaggtg gagccgcacc
     5521 ggtatatgct gcgcccaggc ggcatcttga tcacgcccga cgcggtgcgg gcggaccagc
     5581 tgccgagcaa gaagacgctg aacgtggcct tgcttggcgc cttgagcgca catttgccga
     5641 tggtggaaga gcaatggctc acctccctgc gctcgggctt tgcggagagc ttctttgatg
     5701 gaaaccaaaa ggccttcctg atcggcaggg acagtcagaa accggccaag taagttgagg
     5761 acttcaagat ggacaccgtc aaacgcttct tcaagaacga caagtttgcc gagcgcgcca
     5821 acatcgaact gctctcggtt tcgcccggcc aggcacgggc caaaatgacg ttgcaccggc
     5881 accatatgaa tggttacggg accgtccagg gcggggccat ctttacactg gccgatttcg
     5941 catttgcggc cgcgtcgaac tcacacggca aggtcgccgt ggccatcaac gtgagcatca
     6001 ccttcatgaa ggcgggcgaa agcggcacgc tgtgggccga ggccagggaa gtctccaaga
     6061 acatcaagct cggctcgtac accgtggaag taaaggacga ccagggtgaa ctggtagccg
     6121 tcttccaggg cctggcgtac cggaaatccg aggcgattcc ggtctgagta gtgccgcttc
     6181 cgttcagctc tccagccgct tgaacaggtc cacgctccgg atggggctga tgcccttctt
     6241 ctgcaaggcg gcgatggcgc ggtccgggtc atcgaaccga aacgccagca cccctttgcc
     6301 ttcctgcttg agcgtgaagc cgtacatgta ttcgaggttg acgcgggctt tctccaccct
     6361 caccagtatc tccgccaggc cgccgggttc atcggccact tcgatggcga ccaggtcgga
     6421 gactttgaca atgaagccgt cctcttccag aacttgcttg gccttctccc agtcgcggac
     6481 cacgaagcgc aggatgccga actgccgggt gtcggcgatg gagagcgtca ggatgttgag
     6541 cttcgctttg gcgagcagtt tgacaggccg gctaagcgag ccgagcttgt tttccaggaa
     6601 caatgagagc tgtttgactt tcataggtgg ttctcctcgg ggttacattt tccgctggtc
     6661 aatgactcgt ttggctttgc cttcactgcg ctgaatggta tgcggctgga ccagccgcac
     6721 gttggcccgc acgcccacgg tggtttcaat ggacttgctg agttggctct gcaattgctc
     6781 gagcgcaccc accgtgtcgg tgaagaattc cgaggtgacc tcgacctgca cctccatgac
     6841 gtcgaggtcc ctctcacgcg tcaggacgat ctggtagtgc ggcagcgtgc cttccacttt
     6901 caagagggcg gtctcgatct gggaaggaaa gacgttgacg ccgcgtatga tgaacatgtc
     6961 gtcggcacgg cgcccgatgc gcttgatgcg ccgcagcgtg cggccgcatt cacaggtctc
     7021 ggaagtcagc gccgtgatgt cgcgcgtgcg ataccggatc atcggcatgg ccttcttgcc
     7081 gagcgtagtc aggaccagct cgccttcctc ttcatccggg cagggtttgc cggtcttgag
     7141 gttaatgatc tcggggtaga aataatcttc gaagatatgc ggaccggcct ggcactggca
     7201 ttccatggcc acacccgggc cgatgatttc agacaagccg taaatgtcat aagccttaat
     7261 gccgctgtcc gcttcgatgc gccggcgcat ggattccgtc cacggctcgg cgccgaagac
     7321 gcctacacgc agcggcagtt ccttgaactt cacgccggcc tctacagcct ggtcaatcag
     7381 gtggaggaag tagctcggcg tgcagcagat cgccgtcacg ccgaaatcct tcatcaccat
     7441 caactggcgc ggcgtgttgc cgccggagat cgggatgacg gtggcgccga gcgcttccgc
     7501 gccgtagtgc gcgcccagtc cgccggtgaa caggccatag ccgtaggcat tctggatgac
     7561 atcgccctca tgcaagccgc aagccgcgaa gctgcgcagc atcacgttgg tccagacctg
     7621 aatatcctcc tgggtgtagg cgaccacgat cggcttgccg gtggtgccgc tggagacgtg
     7681 caggcgcaca atctccttca tggggctggc gaacaggccg aaggggtagg tgtcgcgcaa
     7741 gtcggtcttc tccgcgaagg gcaagtggac aatatcgtcc agcgtccgca cgtccagcgg
     7801 ggtcaagccg cgctcatcca tgcgcttgcg gaacagggcc acgcgatcat aagcgcgctg
     7861 gacgatagct gtgagccgct gcaattgcag gtcgcgcaat tgtggcagcg gcagaaaatc
     7921 cggcgcgctg agcgggtgga aacttctgtg ggaactcttt gcgtgtttac tcataaacgt
     7981 tgggcctcgt acgccccgcc agttctacca gattttgcat ttctgatgca actataagtg
     8041 agggggcgcg ggcaaaacgc actattgcct attcacccag cacgccgttt gctagcgtgc
     8101 cagcgaatgg acccaagaat cttaatcgat gggctgatcc tctatctggg cctgcttgtg
     8161 ctgctgacct tccacgagtt cgggcacgcc tggatggcct ggaagtgcgg ggatgatacg
     8221 gcgctgtcgc agggccgcgt ctcgctcaac ccgctggttc acattgacct gatcgggacg
     8281 gtcgccgtgc cgttgctgct gattttcttc gcgcccggcg gctttttcgt cgggtgggcc
     8341 aaaccggttc ccgtggagcc caacaacctc aggaatccgc gactggatga tattatggtc
     8401 acgctggcag gacccttgat gaatttgctg ctggccatcg tcatactggc tttggcgcgc
     8461 ttcgggtatg cggtccattc gcgcggcttg gtggaagtat gcttcaacct ggcgcatctg
     8521 agcctcctgc tgtgtttctt caacctgctg ccgattccgc ccctggacgg ctcgcaggtt
     8581 gcgcgcagcc tgatcgggat gagctacgag acctaccacc agatcgcgcg ctacggcatc
     8641 ttcattctaa tcgtggtatt gcaggtgcca ggcgtgcagc ggaccctgga tgttttaacc
     8701 acgaattcat gggtaagcat tgccggctgg ttcgggatgc cccgcttgat ttagaatcct
     8761 ttccaccacc gggcgttctt ggcatcttca ggatggtctt tgaatcaaac tatgtatgag
     8821 aacactcttg ctcggcattg acagcggcac ccaatccacc aaagtcctcg tcgtggacgc
     8881 gcgggacggc aaagccatcg cctcggcagc gcaggaatat gagctgattc ccaatctgcc
     8941 gcccggcgcg aaggagcagc acccgcacac gtggcgtgag gccaccgcca gcgccatgcg
     9001 ccgcgcgctg cgccaggcca aagcggtcgc cgccgaggtg aaagccattg gcgtgagcgg
     9061 acagcagcac ggctttgtgc cgctcgacaa agcgggcgaa gtcatccgcc cggccaaact
     9121 ctggtgcgac acctccactg ccgccgagtg cgaggagatc accgagcagc tcggcgggtt
     9181 gaagcagacc atccgcgcgc tgggcaacgc cgtcctgccc gggttcaccg cgtccaagat
     9241 tctctggctc aagaagcatg aaccgaagaa ctatcagcgg ctggcgacgg tgctattgcc
     9301 gcacgactac ctgaatttct ggctcaccgg cgagaagacc atggaatacg gcgacgccag
     9361 cgggaccgct ctgctcgacg tgcgcaaacg caaatggtcg gaggcggtcc tcaacgccat
     9421 tgatcccgat cttgccggca aactgccccc cttgatgcgc agcgaccggc ccgccggcct
     9481 tctgcaagct tccacggcca agcagcttga tctgaacccc ggcgtgctgg tgagcgccgg
     9541 tggcggagac aatatgatgg gcgccatcgg taccggcaat acgcgcgcgg gcgtcatcac
     9601 cgccagcttc ggaaccagcg gcaccatcta tgcctgctcg gagaagccgg tggttgaccc
     9661 gcaaggcgag atcgccgctt tctgcgactc gaccaaccgc tggttgccgc tcctctgcac
     9721 catgaatgtg actgttgcca ccgaaatggt gcgcgaggat ttcggctggt cgcacgagaa
     9781 gctcggggtc gaggcggcgc gcattccggc aggctcgcgg ggtctgctgt tgctgccgta
     9841 tttcgagggc gagcgcaccc cgaacgtgcc cgacgggacg ggcgtctggt tcggcgtgaa
     9901 ccagaagacc ttcgaggccg gccactttgc ccgcgcggcg atggagggcg tgacgttggg
     9961 tatgaacta
//
LOCUS       DATCGS010000156         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_262001, whole genome shotgun sequence.
ACCESSION   DATCGS010000156 DATCGS010000000
VERSION     DATCGS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262001"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VIL39_03085"
     CDS             <1..350
                     /locus_tag="VIL39_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480441.1"
                     /translation="QFPDIVDASPSPAASEALMGAIFPLVGEMVAELFTPERIRQLTE
                     RLRTYRNARYAAGDKQATTLASGAIVSLGEERDPASSRFLYALGYLSLMKGLERTANS
                     AQQRAPSPDETAG"
     gene            547..984
                     /gene="rpiB"
                     /locus_tag="VIL39_03090"
     CDS             547..984
                     /gene="rpiB"
                     /locus_tag="VIL39_03090"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY5480442.1"
                     /translation="MKIAIGSDHAGFQYKEKIKGFLEGLGHSVTDFGTHSEEPVDYPL
                     FIRPVAEAVARGEFDRGIVLGGSGNGEAMVANRVKGIRCALCWNADSARLGRQHNDAN
                     VLSLGQRMISLETALQIVQIWLETPFEGGRHQRRIELIDAAAE"
     gene            complement(1046..1621)
                     /locus_tag="VIL39_03095"
     CDS             complement(1046..1621)
                     /locus_tag="VIL39_03095"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEY5480443.1"
                     /translation="MIQLTTPLTEAAVRALKVGDEAAITGTVFTGRDAVHKYLHDGGQ
                     LPPGVNLNGGIIYHCGPVVIKDEQGNWKCVAAGPTTSIREEPYQWQIIRDLGLRGVIG
                     KGGMGERTVEACKQHGCVYLHAVGGAAQVLADCIKRVPHVYFMEQFGAPEAIWEFQVV
                     SFPVVVTIDAHGNSLHKEIFAASQAELAKYV"
     gene            complement(1765..2254)
                     /locus_tag="VIL39_03100"
                     /pseudo
     CDS             complement(1765..2254)
                     /locus_tag="VIL39_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
BASE COUNT          410 a          701 c          713 g          430 t
ORIGIN      
        1 agcagtttcc cgatatcgtg gatgcctcac cgagccctgc tgcgagcgag gcactcatgg
       61 gcgctatctt tcccttggtt ggcgagatgg tggccgagct ttttaccccg gagcgcatcc
      121 gacaactgac cgagcggctc aggacttatc ggaacgcacg ctatgctgct ggggacaaac
      181 aggctactac tttggccagc ggcgcgatcg tctccctggg agaggaacgc gatcccgcca
      241 gcagtcgttt cctctacgcc ctcggttacc tctcgctaat gaagggtctg gagagaactg
      301 ccaattcggc gcagcagcga gcaccgtcgc cggatgaaac ggcgggctga cggctgtgcg
      361 cccttttgcc ggtatggatc aagcgctgca actacgtacc aggcgccaat accgtcgcac
      421 cttggatttg tccggcgcgc aggcggttca gcgccaatta aacacgcccg aacccccaac
      481 cggcgcgact gagagctggc gcaggcgcgc cccgcgtggc aagctgaggc cagaaagatg
      541 catcacatga aaatcgccat tggttcggac cacgcggggt ttcaatacaa agaaaagatc
      601 aagggcttcc ttgaaggcct gggccacagt gtcacggact tcgggacgca ttccgaggaa
      661 cccgtggact atccgctctt cattcggccg gtggccgagg cggtggcgcg gggtgaattc
      721 gaccgcggca ttgttctggg cggctcgggc aacggcgagg cgatggtcgc taatcgcgtc
      781 aaaggcatcc ggtgcgcgct atgctggaat gcggactccg ctcgcctggg ccggcagcac
      841 aacgacgcca atgtgctgtc gctcggccaa cgcatgatca gcctcgaaac cgctctccag
      901 attgtccaga tttggctgga aacacccttt gagggcggac ggcatcagcg gcgcatcgaa
      961 ctcattgatg ccgcggcgga gtgatggagt ggtggagtgt tggagtcgtg gctatagaca
     1021 tggcgctcct acggagcttt cccagctaaa cgtacttcgc caactccgcc tggctggcgg
     1081 caaagatctc cttatgcagg gagttgccgt gggcgtcaat ggtcacgacc acggggaagc
     1141 tgacgacttg gaactcccag atcgcttcgg gagcgccgaa ctgctccatg aaatagacgt
     1201 gcggcacgcg cttaatgcag tccgcgagca cctgcgccgc gccgccgacc gcgtgcagat
     1261 acacgcaccc gtgctgcttg caggcctcga ccgtccgctc ccccatgccc cccttgccga
     1321 tgaccccgcg caggcccagg tcgcggatga tctgccattg atacggttcc tcccgaatgg
     1381 aggtcgtcgg gccggcggcg acgcacttcc agttgccttg ctcgtcctta atgaccaccg
     1441 ggccgcagtg gtagatgatg ccgccgttga ggttcacgcc gggcggaagc tggccgccgt
     1501 catgcagata cttgtgaacc gcgtcccggc ccgtgaacac ggtgccggtg atggcggcct
     1561 cgtcgccgac tttgagcgcc cggacggcgg cttcggtaag gggtgtggtg agctggatca
     1621 taagccaatt ggagtgatgg agtgatggag aattggaatg ctgcgcgggg caggggaggt
     1681 tggagcggcg gggcgctggg gcgaggatct gtgttctcca gcactccaat actcctctac
     1741 tccatcactc cgtttcattg ctcagtatag ccatttctgg acttggccgt ccgcatcgag
     1801 cgtcacgccc tggcggcgat aggcccaaca catatagctg atgctgacga aataggacgc
     1861 gggcaggcga ttgagcacgc cgatcttcgt gcccagcaac gtagttttgc cgccgaaacc
     1921 catcgggccg atgccaagct tgttcgccgt ctcgacgatg tcccgttcca gcttgtcgag
     1981 ctccgcattc gggttgatgt cgtcgagccg gcgcagaaac tgccgcttcg aaaactcgta
     2041 accggtggcc cggtcgccgc cgatgcagac gccgaggatg cccggcccgc agcctttgcc
     2101 ttgcgcctgc aagaccgcgt cgagcatcgc cttgcggcag ccgtccaggt cgcggccggc
     2161 cttgagcttc tcgttgggca gggagtattg cgccccgacg ttttcacagc caccgccttt
     2221 gagcaccagc cgtacgctga cctcgggcga cccg
//
LOCUS       DATCGS010000157         5695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_410840, whole genome shotgun sequence.
ACCESSION   DATCGS010000157 DATCGS010000000
VERSION     DATCGS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410840"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1196
                     /locus_tag="VIL39_03105"
     CDS             72..1196
                     /locus_tag="VIL39_03105"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY5480444.1"
                     /translation="MNAADESWEILQQFLPENLEASAREHGAFRRARGEIHSAEVLLR
                     LLLLHVAGGLSLEQAVVRAREHKLVNISAVGLFKRLRSSGPWLVWLTEQLVKTLSADW
                     DDALWKGRPIRVLDATDIEEPGPTGTDWRLHYSLRLPQLSCDFLELTDVHGGETLKRL
                     PVDPGDIVLCDRGYSHREGVAQVLGAGADVVVRLNTGAFPLTDEAGKALTLVKLVGHL
                     KVNQLGEWPVWFEHAGERHKLRLCVLRKSLAATRLAQRKAEESAREKGQQVRPETLRL
                     AAFVLVLTSLPVTWGTALVLELYRARWQVELAFKRLKQLLAAGHVPKTTDPSSRAWLQ
                     AKILTALLIEHMIHAGRFFSPWGYRLPPGAAVAGLQGGAR"
     gene            1659..2414
                     /locus_tag="VIL39_03110"
     CDS             1659..2414
                     /locus_tag="VIL39_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY5480445.1"
                     /translation="METTKLVELLRGSRRMLLFTGAGISTGSGIPDFRGPEGVWKRRQ
                     PVYYHDFMRSEAARVEFWDFKLEGWPGFREARPNATHEAIVDLERAGKVQAVVTQNID
                     NLHARAGTSAERLIELHGTNSFIECQTCGRRSDPAAHFEYFRKTRRPPVCECGGFLKS
                     ATISFGQNLRNEDLERAEAAAQAADLVVALGSTLSVYPAANIPLLAANRGEPYVIINR
                     GLTEHDDLPEVTLRLEGDVGEIFPPAVAAALKS"
     gene            2651..4597
                     /gene="dnaK"
                     /locus_tag="VIL39_03115"
     CDS             2651..4597
                     /gene="dnaK"
                     /locus_tag="VIL39_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY5480446.1"
                     /translation="MAKVIGIDLGTTNSCMAVMEGGEPSVLENSEGRRVTPSVVAFTK
                     TGERLVGDAAKRQAVTNSKNTVYSIKRFMGRKFTEVEEERKRVPYKVVAAPNGDAAVE
                     IEIDGKAKLYTPAEISAMILGKLKTDAEMRLGEKITQAVITVPAYFNDSQRQATKDAG
                     RIAGLEVLRIINEPTAASLAYGLDKKKDEKIAVYDLGGGTFDISVLEIGDGVFEVKAT
                     NGDTHLGGDDWDNRIMDWILDEFKKEHGMDLRKQADALQRIKEESEKAKIALSSSQVY
                     EINLPFVTADASGPKHISMKLSRAKMEQLCDDLFERTITPVKACLNDAGLEARVIDEL
                     VLVGGMTRMPKVVDTAHGLVNKAPHQGVNPDEVVAVGAAIQAGVLKGEVKDVLLLDVT
                     PLSLGIETLGGVFTKLIERNTTIPTRKSEIFSTASDNQPGVEIHVLQGERQLARDNKT
                     IGKFHLTDIPPAPRGVPQVEVTFDIDANGILHVSAKDLGTGKEQKITITASSGLSKDD
                     IEKMRKDAEAHAEDDKKRREEIELRNEADNAVYRSEKMLKDNADKISGDDKGKIETAV
                     KDVKEAIKGADAAVLRTASEKLNEVWQSVSAELYKAAAEKARASKAGQPGPGPETGAE
                     PKGEDKKGKDEGPIIDAEVVDDKK"
     gene            4670..4963
                     /locus_tag="VIL39_03120"
     CDS             4670..4963
                     /locus_tag="VIL39_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5480447.1"
                     /translation="MALNVKPLGDRVLVEPVEEKEVKKGGIIIPDTAKEKPQEGIVRV
                     LGTGKTDDDGKKVPFEVKVGDRVLVSKYGGTEIKLDGKEYKLLGSDDLLAILQ"
     gene            5018..>5695
                     /gene="groEL"
                     /locus_tag="VIL39_03125"
     CDS             5018..>5695
                     /gene="groEL"
                     /locus_tag="VIL39_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5480448.1"
                     /translation="MAAKQLLFDEAARQAILRGVAKLSRAVSATLGPKGRNVVLDKKF
                     GSPTVTKDGVTVAKEIELEDPYENMGAQMVREVASKTSDAAGDGTTTATVLAEAIYRE
                     GLKFVTAGGNPIGIQRGITKAVEAAVAHLDKIAKKVKDKEEIKQVATVSANWDTTIGE
                     IIADAMDKVGKDGTITVEEAKSIETTLEVVEGMQFDKGYLSPYFVTNAETMEAKLEDA
                     YILIFEKK"
BASE COUNT         1272 a         1576 c         1792 g         1055 t
ORIGIN      
        1 cggccccata agcattaacc taataattga ctgttcctgg tgtttgccct actctcggga
       61 gcatgcaaca cttgaacgct gctgacgagt cctgggagat tctgcagcag tttttgccag
      121 agaacctcga agcgagcgcg cgggagcacg gtgcctttcg gcgcgcccgt ggggagatcc
      181 acagcgccga ggtgctgttg cgtctcttgt tgctgcatgt ggcgggcggg ttatcgctgg
      241 agcaggcggt agtacgggcg cgggagcaca agctggtgaa cattagcgcg gtggggttgt
      301 tcaagcggct gcgcagttcg gggccttggt tggtttggct gaccgaacaa ttggtcaaaa
      361 cgctttcggc ggactgggat gacgcgttgt ggaaaggccg tcccattcgt gtgctggacg
      421 ctacggacat tgaggagccc gggccgaccg ggacggattg gcggctgcat tattcgctgc
      481 ggctgccgca gttgagttgt gattttctgg aacttaccga cgtgcatggc ggtgagactt
      541 tgaagcgact accggtggac ccgggcgaca ttgtgttatg tgatcggggt tacagtcacc
      601 gagagggcgt cgcccaagtg ctgggggcgg gagccgacgt ggtggtgcgg ctcaatacgg
      661 gagcttttcc tctgaccgac gaagcgggca aagccctgac tttggtgaag ttggtggggc
      721 acttaaaggt caatcaactg ggggaatggc cggtctggtt tgagcacgcg ggcgagcggc
      781 acaagttgcg gctgtgcgta ttgcgcaaga gtctggcggc gacccggttg gcccaacgca
      841 aggccgagga aagcgctcgc gagaagggcc agcaagtccg accggagacc ctcagactgg
      901 ccgcctttgt cctggtgttg acttccttgc cggtcacttg gggaaccgcg ttggtgttgg
      961 aactctatcg cgcccgctgg caggtggagt tggcgttcaa acggctcaaa caactcctgg
     1021 ccgctggtca tgtgcccaaa accaccgatc cgtccagccg cgcctggctg caagccaaga
     1081 ttctgacggc cttgctcatc gagcacatga ttcatgccgg gcgttttttt tccccctggg
     1141 gctatcggct gccgccagga gcggcggtgg ctggtcttca aggaggtgcg agatagtgtg
     1201 gttagtgtgc tgcaaatgcc gctgagtctg cgacatctgc tgcgccgggg cagccacatc
     1261 gccgaagcgc tgatggaaaa ccgtccgcgg cgaccgcgcc aattacagcg actcaaaaaa
     1321 ctcgtgctct gctaggttaa tgcttatgag gtccaggggg gtaccaccct aaagccaccc
     1381 caaagccacc ccaaagccaa gggattggga ggaactgtgt tgtgcatccc tcgtgctccc
     1441 gtgagagctt cggtcgtgtc acgaggtctc caccggggcc gtgcgtggct ttgacagcac
     1501 tggtttggct ggcattcgaa aggagtcgca ttttcctgag tcaccttcct tctgctcccg
     1561 cccttgccgg acgcttccgc tttcttcatt cggccttccg ccttctgcct tctttctttt
     1621 tgtttggtgt cgcgaggctt tggggagaaa ctgggggcat ggaaacaacg aagctggtgg
     1681 aactcttgcg cgggtcgagg agaatgctgc tgttcaccgg ggcgggcatt tcgactggca
     1741 gcggcatccc ggatttccgc gggccggaag gggtttggaa acggcggcag ccggtctatt
     1801 accacgattt catgcgctcg gaagcggcgc gggtggagtt ttgggatttc aaactggaag
     1861 gctggccggg gtttcgggag gcgcgcccga acgcgacgca tgaggccatc gtggacctgg
     1921 aacgggcggg caaggtgcag gcggtggtaa cgcagaacat tgacaatctg cacgcgcgag
     1981 ccggcacgtc ggcggagcgg ttgatcgagt tgcatggcac aaacagcttc atcgagtgcc
     2041 aaacgtgcgg gcgacgctcg gaccccgcgg cgcacttcga gtatttccgc aagacgcggc
     2101 gtccgccggt gtgcgaatgc ggcgggttcc tgaagtcggc cacgatcagc tttggccaga
     2161 acctgcggaa tgaggatttg gagcgggcgg aggcagccgc ccaagccgcc gacctggtgg
     2221 tggccttggg ctcgacgctg tccgtctatc cggcggcgaa cattccgctg ctggccgcga
     2281 accggggcga accctacgta atcatcaatc gcggtctgac agaacatgat gacctgccgg
     2341 aggtcacgct gcggttggaa ggggatgtgg gtgagatttt tccgccggcg gtggcggcgg
     2401 ctctcaagag ttaggcaggc tcacaaagag cgacagaatt ctgcctgggg ccagaccgat
     2461 tgtgccagaa ggagtgtgcc acaggtctgg aagttgtgcc aggatggcgc aactggcaca
     2521 actttatgcg cctggcccaa tgaggtgaac tttcgtaagg tgctatttgg aggccactta
     2581 acaagaaatc acccggatgg cactgaccgt gctttagaag gtttggattt ttgtatctga
     2641 acgcatctat atggcaaaag ttataggcat tgatttgggc accacaaact cctgcatggc
     2701 ggtcatggaa ggtggcgaac cttccgtgct ggagaattct gaaggccgcc gggtcacccc
     2761 gtccgtcgtg gccttcacca agaccggtga gcggctcgta ggcgatgccg ccaagcggca
     2821 agccgtcacc aactccaaga acaccgttta ctcaatcaag cggttcatgg ggcgcaagtt
     2881 cacggaggtg gaagaggaac gcaaacgcgt cccttacaaa gtagtcgccg caccgaatgg
     2941 ggatgccgcc gttgaaatcg agatcgatgg caaggccaag ctatatacgc cggcggaaat
     3001 ctccgccatg attctcggca agctcaagac cgatgccgaa atgcggctgg gtgagaagat
     3061 tactcaggct gtcattaccg ttcccgctta cttcaatgac tcccagcggc aagccaccaa
     3121 ggacgccgga cgtatcgccg gcttggaagt gctgcgcatt attaacgagc ccaccgccgc
     3181 ttccctggcc tatggcctgg acaagaagaa ggacgagaag atcgcggtgt acgacttggg
     3241 cggcggcacg ttcgatattt cagtgctgga aatcggcgac ggcgtattcg aggtcaaggc
     3301 gaccaatggc gacacccacc tgggcggcga cgattgggac aaccgcatca tggactggat
     3361 tctggacgag ttcaagaaag agcacggcat ggatctgcgc aaacaggccg acgcgctcca
     3421 gcgcatcaaa gaggaatccg agaaggccaa gatcgctctg tccagttcgc aggtctatga
     3481 gattaacctg ccgttcgtca ccgccgacgc gagcgggccc aaacacatca gcatgaagct
     3541 gagccgcgcc aagatggagc agctctgcga cgatcttttc gaacgcacca tcaccccggt
     3601 gaaagcttgt ctgaacgatg ctggcctgga agccagggtc attgatgaac tggtgttggt
     3661 cggcggcatg acgcgcatgc ccaaggtggt ggatactgcc cacgggttgg tgaacaaggc
     3721 ccctcatcag ggcgtaaacc cggatgaagt ggtggccgtc ggtgcggcca ttcaggccgg
     3781 cgtgctcaaa ggcgaggtca aagatgtcct gctgctcgac gtgacgccgc tatcgctcgg
     3841 tatcgaaacg ctgggcggcg tgttcaccaa gctcatcgag cgcaacacga ccattcccac
     3901 ccgcaaatcg gagattttct ccaccgcctc agacaatcag ccgggcgtgg agatccacgt
     3961 tctccaaggc gagcgccaac tggcccgtga caacaagacc atcggcaagt tccacctgac
     4021 agatatcccg cccgcgccgc gcggcgtgcc gcaggtcgaa gtgaccttcg acatcgacgc
     4081 caatggcatc ctgcacgtga gcgccaagga cttgggtacg ggcaaggaac agaagatcac
     4141 catcaccgcc agcagcggcc tcagcaaaga cgatatcgag aagatgcgca aggacgccga
     4201 ggcgcacgcc gaggacgaca agaagcgccg cgaggaaatc gagctgcgca acgaggcgga
     4261 caacgccgtc tatcgttccg agaagatgct caaagacaat gctgataaga tttccggcga
     4321 cgataagggc aagatcgaaa cggcggtcaa ggacgtgaag gaagccatca aaggcgccga
     4381 cgccgctgtc ctccgcaccg ccagcgagaa gctcaacgaa gtctggcagt ccgtctcggc
     4441 cgagctttac aaagcggccg ccgagaaggc gcgcgctagc aaagcgggcc agcccgggcc
     4501 agggccggaa accggcgccg aacccaaggg ggaggacaag aaaggcaagg acgaaggccc
     4561 aatcatcgac gccgaagtgg tggacgataa gaagtagatt ttcccgcagc gtgtgcggtg
     4621 ggattttgat tcaacgcata atcataaact aaaagaacaa acacaaacta tggcattaaa
     4681 cgtaaaaccc ctcggtgatc gcgttctagt tgaaccggtc gaagagaagg aagtaaagaa
     4741 gggcggaatc attattcctg ataccgccaa ggaaaaaccg caagaaggta ttgttcgcgt
     4801 gctcggcacg ggcaaaaccg acgacgacgg caagaaagtc ccgttcgaag tcaaggtggg
     4861 cgaccgcgtg ctcgtcagca aatacggcgg cactgaaatc aagctcgacg gcaaggagta
     4921 taagctcctc ggctcggacg atctcttggc catcctccaa taaccatcaa cggttgagtt
     4981 tatcaactat caacaatcga ctatttatta aataattatg gcagcaaaac aattactctt
     5041 tgatgaggcc gcacgacagg cgattctccg cggggtggca aagctctccc gcgccgtaag
     5101 cgccacgctc ggacccaagg gccgcaacgt cgtactcgat aagaaattcg gttcccccac
     5161 tgtgaccaag gacggcgtca ccgtcgccaa ggaaatcgag ctggaggatc cttatgaaaa
     5221 catgggtgcc cagatggtgc gcgaagtcgc cagcaagacc agcgacgccg cgggcgacgg
     5281 aaccaccacc gcgacggtgc tggccgaagc gatttatcgc gaaggcttga agttcgtgac
     5341 cgccggcggc aacccgatcg gcatccagcg cggcatcacc aaggccgttg aggccgccgt
     5401 cgcgcacctc gacaaaatcg ccaagaaggt gaaggacaag gaggagatca agcaggtcgc
     5461 caccgtttcc gccaactggg ataccaccat cggcgagatc atcgccgacg cgatggacaa
     5521 ggtcggcaag gacggcacca tcaccgtcga ggaagccaag tccattgaga ctaccctcga
     5581 agtggtcgaa ggcatgcagt tcgacaaggg ctatctctcg ccctatttcg tgaccaatgc
     5641 cgagacgatg gaagccaagc ttgaggacgc ttacatcctc atcttcgaga agaag
//
LOCUS       DATCGS010000158         4667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_209924, whole genome shotgun sequence.
ACCESSION   DATCGS010000158 DATCGS010000000
VERSION     DATCGS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209924"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..574
                     /locus_tag="VIL39_03130"
     CDS             215..574
                     /locus_tag="VIL39_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480449.1"
                     /translation="MKRQSSQSRTTRRHRTLKEKAEIFRAHRRSGLSLLAFAQKQGLC
                     YASLLRWRSRPGKGANVPLAPDTEADPRFVPVKIEGEGLSGEYVLSWAGGRSLKIPRQ
                     FETDSLRRLLTVLEGVR"
     gene            571..849
                     /locus_tag="VIL39_03135"
     CDS             571..849
                     /locus_tag="VIL39_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480450.1"
                     /translation="MIGLLPATTIFLATVPTDLRKAKYGHGLELVETFMDRSRFHGRA
                     YAASNWLEVGLTQGRSRGDREHRIQVPKKAVYVYPLIPNWPANGVMRG"
     gene            846..2033
                     /locus_tag="VIL39_03140"
     CDS             846..2033
                     /locus_tag="VIL39_03140"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY5480451.1"
                     /translation="MTSAPRQDNRLKADVSVIADVVVKKYVEHQPLYRQQQAFDRLGI
                     PITRQTLCYWTGWCSDQVEPVVKAMAAYTRSESLVQSDETSVRMQLADGQMETARLWA
                     YGLPWAEVVFDFRTDKSQAGPKEFLTGTRARFVQADGGSSYGPVFKELDLLHIACMAH
                     IRRGLFEARADAPRAVDLVLAAIQKLYRIESRAKAQAMSLEARLELRQREAKPIFMDL
                     GQLIATLRKEVLPKSPLGRALSYAENQWPAMARYLEAAQAELDNNSTEHALRAVVLGR
                     RNWLHVGQESGGEKAANLFSLMITCKRLQVEPYAYLHDILRRLPSHPTGTSGSSRPGA
                     GKKPSRPRARRPVPAAESYRLSTQSIRSSRATTRRAAGATRFRRTVTYKQQHLQLCRQ
                     PLD"
     gene            2065..2424
                     /locus_tag="VIL39_03145"
     CDS             2065..2424
                     /locus_tag="VIL39_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY5480452.1"
                     /translation="MMQYFGHPTGQASDLSRAQDDVDGDGVSNLAEYLAGTNPKDAHS
                     YLSITGIGLGGTNGGQIAWGSASNKLYSLQRAVALIGGLGFTAIAEHILSTPPENVYL
                     DASATNRPSLFYRVRVE"
     gene            2512..4500
                     /locus_tag="VIL39_03150"
     CDS             2512..4500
                     /locus_tag="VIL39_03150"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEY5480453.1"
                     /translation="MKLNPLTLSAALALILSTAHALAGEPETVWISSLDLSPIAQGWG
                     QAQADKAVTGKPMAIGGQKFERGLGTHADSLVRIDLKRGAVKFSALVGVDDAAGSDHA
                     TLAFRVIGDGKTLFKATGMKLGQAARKVDVDVKGIKMLLLLADSGGDVSYGHADWADA
                     KLLVTGERPVIVGSPREAAVILTPKPARTPRINGARIFGVHPGHPFLFTIPATGDRPM
                     TFAVDHLPKGLTVDASTGQIQGRIEKPGEYVVTFRAANQFGKAKLKFKIVCGGTLALT
                     PHMGWNSWYVWEGHVTDKIMRAAADAMVSSGMMDHGYQYVNIDDCWAVKPGANDPTLG
                     GEPRDAQGKVNANQRFPDMKGLTDYIHAKGLKAGIYTSPGPTTCAGHVGAYQHEELDA
                     QRFAEWGYDFLKYDWCSYGGIAKKPDLAAMQKPYRLIGDILRRQNRDLVLNLCQYGMG
                     DVWKWGKEIGGHSWRTAGDLGGSFYGIPAALFRDGFDVYSSKELHKYGGPGGWNDPDY
                     LLLGYLSNWNGQTAPSPLTPNEQYTHVSLWCLVAAPLIFSGDITRLDDFTLSLLTNDE
                     VIDVDQDPLGKPGRRVAIDGDLEVWVRDMEDGTKAVGLFNRGEDATTVTAKWADLGLK
                     GKRIVRDLWRQQDLGKYEGEFKTSVPRHGVVLVRLRAK"
BASE COUNT          903 a         1384 c         1524 g          856 t
ORIGIN      
        1 cccctttgag cgcttccgcg tccccggcag catgtctgcg agttcaaagc tttccgccgt
       61 tctcgggtgg cagggcccag ccgctttggg ggtctcccgt tgccgaacgg gtagaatctg
      121 gctcctccaa tagtgtcaat agtgagcatt ggcaacgtaa ccgtccggcg gagccgtctt
      181 acactgagcg tggtgggtct ggcaggatgg cggggtgaaa cgacaatcat ctcaatcccg
      241 cacaacccgg cgacaccgca ccctcaaaga gaaagctgag attttcagag cccaccggcg
      301 cagcggactc tcgctgctgg ccttcgccca aaagcaggga ctgtgctacg cgagcctgct
      361 gcgctggaga tcccggccag gcaagggagc caacgttccc ctggcgcctg acaccgaagc
      421 cgatccacga ttcgttccgg tgaagatcga gggtgagggg ttgagcgggg aatacgtgct
      481 gagctgggcc ggaggacgtt ccctgaagat cccgcggcaa ttcgagacgg attctttacg
      541 tcgattgctg acggtgttgg agggagtgag atgatcggcc tgttgcccgc gaccacgatc
      601 ttcttggcca cggttcccac cgatttgcgc aaagccaagt atgggcatgg ccttgagctg
      661 gtggaaacgt tcatggatcg gagccggttc catgggcgtg cttacgcggc cagcaattgg
      721 ttggaggtgg gactcactca agggcgcagt cgcggggatc gggaacaccg gattcaggtt
      781 cccaagaaag ccgtgtatgt ctatccgctt atccccaact ggcctgccaa cggtgtcatg
      841 agggggtgac cagcgcgccg cggcaggaca accgcctcaa agccgatgtg agcgtgatcg
      901 ccgatgtggt ggtgaagaaa tatgtggagc accagcccct ttaccgtcag caacaagcct
      961 ttgaccgatt agggatcccg atcacccggc agacgctctg ctactggact gggtggtgca
     1021 gcgatcaggt cgagccggtg gtcaaagcga tggcggctta cacccggtcc gaatcgttgg
     1081 tccagtccga cgagacctcg gtgcggatgc aattggccga tggccagatg gagacggcac
     1141 gactctgggc ctacgggttg ccgtgggcgg aggtggtctt tgattttcga acggacaaaa
     1201 gccaggccgg tcccaaggag ttcctcaccg ggaccagggc tcgctttgtc caagccgacg
     1261 ggggcagttc gtatgggccg gtgtttaagg aattggattt actgcacatc gcctgcatgg
     1321 cgcatattcg tcgcggcctc ttcgaggctc gagccgatgc gcccagggcc gtggatttgg
     1381 tgttggcagc gatccagaag ctctatcgga tcgagagccg ggccaaggcg caggccatga
     1441 gtcttgaagc ccgcctggaa ctgcgacaac gggaagccaa gccgatcttc atggacctgg
     1501 gccagctcat cgccacgctg cgcaaggagg tgcttcccaa gagtcctttg ggccgggcgt
     1561 tgagctatgc ggagaaccaa tggccggcga tggctcgata cctcgaggcc gctcaagcgg
     1621 agttggacaa caactcgacc gagcatgctc tcagagcagt ggtcctggga cgacgcaact
     1681 ggctgcacgt cggccaagaa tcagggggag agaaggcggc caatctcttc tcactgatga
     1741 tcacttgcaa acgactgcag gtggaaccgt atgcctacct ccacgacatc ctgcggcggc
     1801 tgcccagtca tccaacaggg acatctggca gctcacgccc aggggctgga aagaaacctt
     1861 cgcgcccaag agcccgccgc cctgtcccag cggctgaatc ctaccgactc tcgactcagt
     1921 cgattcgctc gtcccgtgca actacccgac gcgccgccgg tgccacacgg ttccgccgga
     1981 cggttacata caaacaacaa cacctccagc tttgccgcca acctttggat tgattcggat
     2041 ggcgatggca taccggacaa ctggatgatg cagtatttcg gccatccaac cgggcaggcg
     2101 agcgacttgt cgcgcgcgca ggatgatgtc gacggggatg gcgtgagcaa cctcgcggaa
     2161 tatcttgccg gcactaaccc gaaggatgcg cattcgtatc tgagcatcac ggggatcggc
     2221 ctcgggggaa caaacggggg gcagattgcg tggggctcgg cgtccaacaa actctattca
     2281 ctccagcggg ccgttgctct gatcgggggc ttgggcttta cggccatcgc agagcacatt
     2341 ctgtccacgc caccggagaa tgtctatctg gacgcgagcg caaccaaccg tccgtcgctg
     2401 ttctaccggg tgagagtgga gtagggcaac tggtcgagcg gggctgaggc acgcgtccgc
     2461 gcgaaacttt gcgctggaag tcatctgttc catcggggaa tatgcgcaac catgaaactg
     2521 aacccgctta cgctctccgc cgcgctggcg cttatcctat ctaccgccca cgctctggcc
     2581 ggcgaacccg aaacggtctg gatttcctcg ctggacctct ccccaatcgc gcagggctgg
     2641 ggacaagccc aggccgacaa ggcggtcacc ggcaagccga tggcgatcgg cgggcagaaa
     2701 ttcgaacgcg ggctgggcac gcacgccgac agcctcgtgc ggattgatct taagcggggc
     2761 gcggtgaagt tctcggcgct cgtcggggtg gatgatgccg ccggcagcga tcacgccacc
     2821 ctcgcattca gggttattgg tgacggcaag acgctgttca aggccaccgg gatgaagctc
     2881 ggccaggctg cccgcaaggt ggacgtggac gttaagggaa tcaagatgtt gctcctcctc
     2941 gcggattccg ggggcgatgt ctcctacggt cacgctgatt gggcggatgc caaattgctc
     3001 gtcacgggcg agcgtccggt cattgtcggc tcgccgcgtg aagcggctgt catcctcacg
     3061 cccaagcccg cccgcacgcc gcgcatcaac ggggcgcgca tcttcggcgt ccacccgggg
     3121 cacccgttcc tgttcaccat tccggcgacg ggcgaccgcc cgatgacgtt cgcggtggat
     3181 catctgccga agggcttgac cgtggatgcc agcaccggcc aaatccaggg ccggatcgag
     3241 aagccgggcg aatacgtcgt gacgtttcga gccgccaacc agtttggcaa ggcgaagctc
     3301 aagttcaaga ttgtctgcgg cgggacgctg gccttgacgc cgcacatggg ctggaacagt
     3361 tggtatgtgt gggaggggca cgtgacggat aagattatgc gcgccgccgc cgacgccatg
     3421 gtgagcagtg gcatgatgga tcacggctac caatacgtga acattgatga ttgctgggcg
     3481 gtgaaaccgg gcgccaacga cccgacgctg ggcggtgagc ctcgcgatgc gcagggcaag
     3541 gtcaatgcca accagcgttt cccggacatg aagggtctga cggactacat ccacgccaag
     3601 ggcctcaagg cgggtatcta cacctcaccg gggccgacga cgtgcgcggg ccacgtcggc
     3661 gcgtatcaac atgaggaact ggacgcccag cgcttcgccg agtggggtta cgacttcctt
     3721 aagtatgatt ggtgttcgta tggcgggatt gcgaagaagc cggatcttgc cgccatgcaa
     3781 aagccgtatc gtctcatcgg tgacattctg cgccggcaga accgcgacct ggtgctcaac
     3841 ctgtgccagt acggcatggg tgacgtttgg aaatggggga aggaaatcgg cgggcacagt
     3901 tggcgcacgg cgggcgacct gggcggctcc ttttatggca tcccggccgc gctgttccgg
     3961 gacggctttg acgtctattc cagcaaggag ctgcacaagt atggcggccc gggcgggtgg
     4021 aacgatcccg actacctgtt gctcggttac ctgagcaatt ggaacggcca gaccgcaccc
     4081 agcccgctca cgcccaacga gcaatacacg cacgtttccc tctggtgcct ggtggcggct
     4141 ccgctcattt tcagcggcga catcacccgc ctggatgact tcaccttgag cctgctgacc
     4201 aatgacgagg tcattgacgt ggaccaggac ccgctcggca aaccgggtcg ccgggtggct
     4261 atcgacggcg atctggaggt ctgggtcaga gatatggaag acggcacgaa agcggtcggc
     4321 ctgttcaatc gcggcgaaga cgcgacgacc gtgaccgcca aatgggccga ccttgggctc
     4381 aagggcaagc ggatcgtgcg cgacctgtgg cggcaacagg acctgggcaa gtatgagggc
     4441 gagttcaaga cctccgtgcc gcggcatggg gtggtgctcg tgcggctgcg ggcgaaatag
     4501 actccttctg ggcgcccact ggtgttggct gcgcccacat ttcaagacgc gctcccatga
     4561 ctttgacact gacagtgcgc gggatagtgg ggcgggcgtc ccgcctgcct tctcgggcgt
     4621 ctcgcccgcg ttggaggatt cgagggcgag acgccctggt ggcaggc
//
LOCUS       DATCGS010000159         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_460826, whole genome shotgun sequence.
ACCESSION   DATCGS010000159 DATCGS010000000
VERSION     DATCGS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460826"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1863)
                     /locus_tag="VIL39_03155"
     CDS             complement(298..1863)
                     /locus_tag="VIL39_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5480454.1"
                     /translation="MPATKETDSLDSKFYKPADAFFPVFSSLGLTYDDVTLATRYSEV
                     LPRDTQLDTTLAEGLQLNIPVISADMDTVTESRLAIALALNGGLGLIHYNMPEREQLS
                     EVSRVKNNVHGLIQEPIKVAPEQLIGEVLELIAKKRFAFSTFPVVDGQGKLVGLLSGQ
                     VVKPRYAKRKVAELLTPRSQVHTLTKKELGRDPIAKADKFFTDHPGINKLLMVDDQDR
                     LHGLFTMSDVERITQERNAQFKPARDAHFRLLCGAAVSATRNAFGELDRERILTHVGA
                     LVERGMDAVAVSTAHGHSRGVGDAVRMLRDAFPKLPIIAGNVTSAAAVEFLADCGANA
                     VKVGQGPGSICTTRIVAGVGIPQLTALYLASRAAQKKKVTILADGGITKSGDIVKALT
                     LAHAVICGGILAGCPEAPGDVMEIGGKLYKKYRGMGSLAAMKEGSAARYGHTVNPASK
                     VAPEGVEALKEVSGSVDRVLAQLIGGIQSGMGYLGAANLAQLREQARFIRVSPAGQRE
                     AAPHDVMELKTGS"
     gene            complement(2319..>2545)
                     /locus_tag="VIL39_03160"
     CDS             complement(2319..>2545)
                     /locus_tag="VIL39_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt ABC transporter ATP-binding protein"
                     /protein_id="HEY5480455.1"
                     /translation="REFKALLREIPATKLIATHDLELVVELCSRAIVLDHGAVVADGP
                     VTELLNNEELMLAHGLERPHILRHQHPHEQ"
BASE COUNT          443 a          848 c          771 g          483 t
ORIGIN      
        1 gtgtgggcat ctactccgag ctgcgccggc ggcggttcga gccgtctccg agcgaagacc
       61 acatcttccg ctgccgccgc tgcggctatg tctataccga cgatccggac gtggaccgtt
      121 cacgctgtac gcaatgcggg cagatgaaca aggccattga attctgacgg agcaacgacg
      181 gagcaacgga gtaatggagt ggaggagtat tggagtgatg cgaaagacat ctctgccctc
      241 ccatccctcc accactccac cactccacta ctccattact ctatgactcc acttcgctca
      301 gcttcccgtc tttagctcca tcacatcatg cggcgccgct tcgcgctggc cggcggggct
      361 gacacggatg aaacgggctt gttcgcgaag ttgtgcgagg ttggccgcgc cgagatagcc
      421 catgccagat tggatgccgc cgatcagttg cgccaggacg cggtccaccg agccgctcac
      481 ctctttcaat gcctcaacgc cttcgggggc aaccttgctg gccgggttaa ccgtgtggcc
      541 atagcgcgcc gcggagcctt ccttcattgc cgccagactg cccatgccgc ggtatttctt
      601 gtagagcttg ccgccgatct ccatcacgtc tcccggcgct tccgggcagc cggccaggat
      661 gccaccgcag atgaccgcat gggccagtgt gagcgccttc acgatgtccc ccgacttggt
      721 gatgccgcca tcggcgagga tggtaacctt cttcttctgc gccgctcgcg acgcgaggta
      781 gagcgccgtg agttggggaa tgccgacgcc ggccacgatg cgcgtcgtgc aaatcgagcc
      841 cggaccctgg ccgactttga cggcgttggc gccgcaatcg gcgaggaact ccaccgcggc
      901 ggcgctggtc acgttgccgg cgatgatggg cagcttggga aacgcatcgc gcagcatccg
      961 caccgcatcg cccacgcccc ggctgtgccc gtgcgcggtc gagacggcca ccgcgtccat
     1021 gccgcgctca accagagcgc ccacgtgggt caggatgcgt tcacgatcaa gctcgccaaa
     1081 cgcgttccgg gtggcggaca ccgccgcccc gcacaggagg cggaaatgcg cgtcgcgcgc
     1141 cggcttgaac tgcgcgttcc gctcctgcgt gatgcgctcg acatcgctca tcgtgaacag
     1201 cccgtgcagc cggtcctggt cgtccaccat cagcagcttg ttgatgccgg ggtggtcggt
     1261 gaagaacttg tcggccttgg caatggggtc acggcccagt tccttcttag tgagcgtgtg
     1321 gacctggctg cgcggcgtga gcaactccgc gaccttgcgt ttggcgtaac gcggcttcac
     1381 gacctggccc gagagcagcc ccaccagctt gccctggccg tccaccaccg gaaacgtgct
     1441 gaaggcaaac cgcttcttcg caatcaactc cagcacctcc ccgatcaact gctcgggcgc
     1501 cactttgatg ggctcctgga tcaggccgtg gacgttgttc tttacgcggc tgacctctga
     1561 aagctgctcc cgctccggca tgttgtaatg gatgaggccc agcccgccgt tgagcgccag
     1621 cgcgatggcc aagcgtgact cggtcaccgt gtccatgtcc gcggagatca ccgggatgtt
     1681 gagctgcaaa ccttccgcca gggtcgtgtc gagttgcgta tcgcggggca agacttcgga
     1741 ataacgcgtc gccagggtca cgtcatcgta ggtgagtccg aggctgctga agaccgggaa
     1801 aaaggcgtcc gccggtttgt agaacttgga atcgagactg tcggtttctt tggtcgctgg
     1861 catgtcagaa cgtgccagag aggacctgcg cctggcaagt ccaaacacgc cccgaaagag
     1921 tagtggggag agcatcgcct tttctgaccc ccctctgctg accgagtgga atggaccaag
     1981 gcgagactcg atcgtcgaag ccgaggctgc ggcgccaggg tggcgtccag gccccggaaa
     2041 gcgccctggt ttcgagccag ctcttcgtgt cgcccggacc gtctagagac cgtaaccagc
     2101 gcctgatttc caggccatcc cttcgtaact ccatcgtaac cccttcgaaa ccccttcgaa
     2161 accccttcga agcatcggca gagcagcggg cgagcaaccc gctggcaggg ccgtggcagc
     2221 aagcccgcca gacgccccat tccaccgcgg aagaagccgt tttgcgaggc aacgcgcgtc
     2281 ctccatttca gcctggtttc tgccttcggc cttccgcctt actgctcatg cggatgctga
     2341 tgcctcagga tgtggggccg ttccaggcca tgcgccagca tcagctcctc gttgttgagc
     2401 agctctgtca cggggccatc cgccaccacc gccccgtggt cgagcacaat ggcccgtgaa
     2461 cacagctcga cgaccagttc caggtcatgc gtcgcgatca gtttggtcgc cggtatctcc
     2521 cgcagcagcg ctttgaactc gcgcc
//
LOCUS       DATCGS010000160         6011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_708247, whole genome shotgun sequence.
ACCESSION   DATCGS010000160 DATCGS010000000
VERSION     DATCGS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708247"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..3356
                     /locus_tag="VIL39_03165"
     CDS             561..3356
                     /locus_tag="VIL39_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY5480456.1"
                     /translation="MNTRIRLIKHHTNRCFALARFSSRERRFPIRSVRTLAAAAGLCL
                     ALAGPAQAAMVGHWTFDDSANPWAETSGYKPAGTHDGLPVNSAAWSAEGHAGGRTGGS
                     LDLTAGGCALRIMNTSQTLDAGYQATFDDGLENGVTIAFWAKGFPDTWCSWVSKNGEA
                     FGYQVRRWGSENYATFTLRGTSGTPDPSSTSITVNSSGNSWHHYGAVWDAANGTRQLY
                     VDGILSISITGDSGPYTLANNMCLAFGAKDWDGGQGGNFVAWTTCKMDDVRIYDAPLS
                     AAEVQALAAQEAGTMILYPQWAETLPGQKTIFSVTLVPQALQSSGVTVWVTNSSPASV
                     TIPGAVGNVLKLTFAQGSTNVQTFQAVSSAPGTVNLTCGASDGALGNAAAVQVDTPLP
                     PALVAHWTFDDQANPYVETSGYQAAGTHDGSAQGTVATSTDIPPGTSGNSLDLTQGGS
                     VQIGNTKSGDAGYEPTFDGVLASQMTIAFWVKVAQQMSSWDPFIVKNGEGPGFQVRFW
                     DVTPHGCFTVRGTDGPGEVGGGVTIGDGQWHHIAAVRNGMAGLRMMYVDGYPDQASVL
                     PTSDTGLMQPASADSLYLGPATGSGRQFNGYLKDVRVYNYPLTRAQVQALLPSVPTPM
                     AGHWTFDAAAPWADSSGYKPAGTHDLLPVGAVSFSADVPPGRTGQSLDLTAGGSAVVV
                     SNSNQQMGGAGGGDPWTANPTWQNSFDVGLSMQMSIAFWAKGFPDAGNPWVAKKGTDY
                     GYQVARSGSDNFATFTQTGTPGIDSPAGTMLNVNDNAWHHYAAVWDGIAGTRQLYVDG
                     TIDPGVNLTGDYGPSVNIASFEYLAFGARDLGGVGSFTPCLLNDVRVYRMALSQAEVL
                     ALLPASPAPTLASRNAGASGLRIAWPVASFGYRLQTSASLTGAWNDAGLIVSVEGNER
                     AAYAPVAASAQFFRLVK"
     gene            3443..5995
                     /locus_tag="VIL39_03170"
     CDS             3443..5995
                     /locus_tag="VIL39_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEY5480457.1"
                     /translation="MAVLLCGYAAAASEIPPGRLAPGAPVLEANPTNVAKLLAAVDTW
                     LLPQPKAVAMSGEGFDLKQCRGIRLLGCDDPRLKTDFPLLLQERCGIRLKASIGKPGR
                     GWISLVLCPNETPPAGVKSFSRADLAGLGEQGYCLRVERSGITAAAATGQGLYYAART
                     LAQLANGRTRLPGMFIRDWPSLRYRGFQYDISRGQMPKLESLKRLAQVTAEAKMNLYE
                     LYIEHEFAWQRYPDIAPPEALTRAEGRELFDCAARYHMDVHPLMQVFGHFYNIGLKPP
                     YRKFMVEDGGTVDIRKPEAVAFVTNLVDEVCDALPGKFLNVDITEIDDAAFKKTGTTQ
                     AELNELTFQYALKIREAAARHGMRLMVIQAQLGAEGSLAGLGSVVERLPKDILIGCYY
                     TAEFYGGWEKDFPRLQRLGLDFLAQPWIDSHGHIMPYVGHAMDFSDIEISRGLQYGAV
                     GSTTCDWGDDGHYHLPALTWFPFVYHAASAWTGAKLDRDYFNRAFCRIFFGTADDSIA
                     RAILLVGNINGQKLKLRNAAGGIDEPAYTGNSTFGRYYYEFFGDPFTDPKVLDIVEPG
                     RTGQGILQPALEAEKLLEQARGQVRQNQDVLERLLFTSRSYEAMGRKLIARSHFLDNN
                     VPRAQVAEELLSLAKTYEGLKADFQRLWLADCQDAGSFRGYIQRFDNTIVPCRKKAEE
                     LTRMSSTKVAADVSRRILAEAKTARTDVGGYPLAADPATGEISKLPRKEKAVLVGVNY
                     FPGWWRPHPNKWQDRRGEDWRLRFPERVPLLGEYNEQETMNREIAAAAAAGVDFFSIL
                     WYYNPPGHEREPNVALTESGLANFMASPEARRLRFMITRAALSTCGAQKANS"
BASE COUNT         1129 a         1866 c         1960 g         1056 t
ORIGIN      
        1 gggaggtcgg ccggggtctc agagcgtggc acgcgccgca gaagcagcct ggaaccagac
       61 cctgcagggt cccgccgcac taactacgag ggccaagcgg gtaggggagt tcaacgacaa
      121 gaagaacgcc ccgaagccag acagggagtc ggattcgtcc atagtagccc gtggcaagac
      181 gcccgtctgg aagcgggtga aggggcgaac aggctaacga agcccgcaca ggcaaccaga
      241 tgccgtaaga atgacggatt aacactggca aaccttcctg cgggcgatac acggcgtgtt
      301 agcttgaaga gcccggtgcg ggcaaatcgc acgccgggat ctgtgagggg ggcgccgggc
      361 aaccggtgtc cctacctcga catcagttca gtagttgaaa ctgatggctt agccggcctg
      421 cgggagcggc ctgcggctga cggaattgct ggaatgttct ggacatgacg ggttgatggt
      481 tactattctc gcacgtgtag tatcgtcatt tcgcttttgc gatggccgca gtaacaactg
      541 atgcaacaaa accgatagtc atgaatacaa gaatccgtct aatcaaacat cacacaaacc
      601 gttgcttcgc ccttgcccgc ttctcgtcaa gagagcgccg ctttccgatc cgctccgtcc
      661 ggacgctggc ggcggcggcg ggcctctgcc tggccctggc cgggccggcg caggcggcga
      721 tggtcggcca ctggaccttc gacgattccg ccaacccgtg ggccgagacc tccggctaca
      781 agccagcggg cacccacgat ggtcttccgg tcaacagtgc cgcctggtcc gccgaaggcc
      841 atgcgggtgg gcgcacggga ggttcgctgg acctcaccgc cggtggctgc gcgttgcgca
      901 tcatgaacac cagccagacc ctggacgcag gttaccaggc gacgtttgat gacggcctgg
      961 agaatggggt gacgatcgcg ttctgggcga agggctttcc cgacacctgg tgctcttggg
     1021 tttcaaagaa cggcgaagcc tttggctacc aggtccggcg ctggggctcg gagaattacg
     1081 ccaccttcac tctgcggggc acatcgggca cccccgaccc gtcgagcacc agcatcacgg
     1141 tgaacagtag cggcaatagc tggcatcact acggcgccgt ctgggacgcc gctaacggca
     1201 ctcgccagct ctacgtggat ggcatcttgt ccatcagcat caccggcgac tccgggcctt
     1261 atacactggc gaacaacatg tgtctcgcgt tcggcgcaaa ggactgggac ggaggccagg
     1321 ggggtaactt tgtcgcctgg accacctgca agatggacga cgtgcgcatt tacgacgcgc
     1381 cgcttagcgc cgccgaggtg caggccctgg cggcccagga agcgggcacg atgattctct
     1441 atccccagtg ggcggagacc ctcccggggc agaagaccat cttcagcgtc accctcgtcc
     1501 cgcaggcgtt gcagagcagc ggcgtcacag tttgggtcac caattccagc ccggcttcag
     1561 tgacgatccc gggcgcggtg ggcaacgtgc tgaaactcac cttcgcccag ggttccacca
     1621 acgtgcagac gttccaagcg gtcagcagcg ccccaggcac agtcaatctg acctgcgggg
     1681 cttccgacgg cgctttgggc aacgcggcgg cggtgcaggt ggatacgccg ctgccgccgg
     1741 ccctcgtggc gcactggacg ttcgacgacc aggccaatcc gtatgtcgag acatccggct
     1801 atcaggcggc gggcacgcac gacggctcag cgcaggggac ggtggcgacc tctactgaca
     1861 ttccgccggg cacgagcggg aattctctgg acctgacgca agggggctcg gtgcagatcg
     1921 gcaatacaaa gtctggtgat gccgggtacg agccgacctt tgatggtgtg ctggcgagcc
     1981 agatgaccat cgccttctgg gtcaaggtgg cgcaacagat gagtagctgg gatcctttca
     2041 tcgtcaagaa tggcgaaggg ccaggtttcc aagtgcgctt ttgggacgtg actccccatg
     2101 gctgcttcac cgtgcggggg actgacgggc cgggtgaagt cggaggtggc gtaacgattg
     2161 gcgatgggca gtggcatcac atcgccgccg tccgcaacgg catggccggc ttgcgcatga
     2221 tgtacgtgga tggctacccc gaccaagcca gcgtcctccc gaccagtgat actggcctga
     2281 tgcagccagc gtccgcggac tcgctttacc tcggccccgc tactggcagc gggcgtcagt
     2341 tcaacggtta tctgaaggat gtgcgtgtct ataattatcc gctgacccgc gcccaggtgc
     2401 aggcgctgct gcccagcgtt cctacgccca tggccggcca ttggaccttt gacgccgcgg
     2461 cgccgtgggc ggatagctca ggctacaaac cagccggcac ccacgacctg ctccccgtcg
     2521 gcgcggtgag tttttcggcg gatgtcccgc cgggccgcac cggccagtcg ctcgacctga
     2581 cggcgggcgg cagcgcggtg gtggtgtcga acagcaatca acaaatgggt ggcgccggcg
     2641 gtggcgaccc gtggacggcg aacccgacct ggcagaacag cttcgacgtt ggcctatcta
     2701 tgcagatgag cattgccttc tgggcgaagg gctttccgga tgcggggaac ccctgggttg
     2761 ccaagaaggg cacggattac ggttaccagg tcgcgcgctc cggctcggac aacttcgcca
     2821 ccttcaccca gaccggcacg ccgggcatcg actctccggc cggcaccatg ctcaatgtca
     2881 acgataatgc gtggcaccat tacgccgcgg tctgggacgg tatcgccggg acgcgccagc
     2941 tctacgtgga tggcacgata gaccccgggg tcaatctcac cggggattat ggcccgagcg
     3001 ttaacatcgc ctcgttcgag tacctggcct ttggcgcgcg cgatcttggg ggagtgggga
     3061 gcttcacacc ctgcctgctc aacgacgtgc gcgtctatcg catggccctc agccaggccg
     3121 aggtcctcgc cctgttgccg gcgagcccgg cgccgacgct ggctagccgg aacgccgggg
     3181 ccagcggtct tcgcatcgcc tggcccgtcg cgagcttcgg ctatcggctc cagacctcgg
     3241 cgagcctgac cggcgcttgg aatgacgcgg ggttgatcgt gtcggtggag ggaaatgagc
     3301 gagccgccta tgcgcccgtc gcggcgagcg cgcagttctt ccgactcgtg aaatgacgtg
     3361 cccggcggaa gcgcgtgctg gtgaagatca tcggggaata gtggagttgc gaaacaaaca
     3421 actaagttgg cgcgctctgc tcatggcggt gctgttatgc ggctatgcgg cagccgccag
     3481 cgaaattccg ccaggccgcc tcgcgccagg cgctccagtc ctcgaagcca acccgacgaa
     3541 cgtcgcgaag ctgctcgccg ccgtcgacac ttggctcctg ccgcagccaa aggccgtggc
     3601 gatgagtggg gagggctttg acctcaagca gtgcagggga atccgtctgc tcggatgtga
     3661 tgacccgcgg cttaagactg atttcccgtt gctgttgcaa gagcgctgcg gcattcgcct
     3721 caaggcgtcc attggcaagc ctggccgagg ttggatttcg ctggtgctgt gtccaaatga
     3781 aacgcccccg gctggggtga agtcgttcag ccgcgcggac ctggcgggcc tgggagagca
     3841 gggctactgc ttgcgcgttg agcgttcggg catcaccgcc gccgctgcga ccgggcaggg
     3901 cctctactac gccgcgcgga cgctggccca gcttgccaac gggcggaccc ggctgccggg
     3961 catgttcatc agggattggc cgtcgctgcg ctaccgcggg ttccaatacg acatcagccg
     4021 gggccagatg ccgaagctgg aatccctgaa gcggctcgca caggtcactg cggaggccaa
     4081 gatgaaccta tacgagctgt acattgagca cgagttcgcc tggcagcgat acccggacat
     4141 cgcgccgccg gaggccctga ctcgcgccga gggccgcgag ctgtttgact gcgccgcgcg
     4201 gtaccacatg gacgtgcatc cgctgatgca ggtcttcggc cacttctaca acatcggcct
     4261 gaagccgcct taccggaagt tcatggtgga ggatggtggc accgtggata tccgcaaacc
     4321 ggaggcggtg gccttcgtta cgaacctggt tgacgaagtc tgcgatgccc taccgggcaa
     4381 gtttctcaac gtggacatta ccgagatcga cgacgcggcc ttcaagaaga ccgggaccac
     4441 ccaggcggaa ttgaacgagc tgaccttcca atacgcgctc aagatccgcg aagccgccgc
     4501 ccggcacgga atgcgcctga tggtcatcca ggcccagctc ggcgccgaag gttccctggc
     4561 cggcctgggc tcggtggttg aacggctgcc caaagacatc ctcatcgggt gctattacac
     4621 cgctgagttt tacggcgggt gggagaagga cttcccgcgg ttgcagcggc tcggcctgga
     4681 cttcctcgcg cagccctgga tcgactcaca tggacacatc atgccttacg tcggccacgc
     4741 gatggacttt tctgacatcg agatcagccg agggctgcaa tacggtgcgg tgggcagcac
     4801 cacctgtgac tggggcgacg acgggcatta ccatctcccg gcgctgacct ggttcccgtt
     4861 cgtctatcat gccgcgtccg cctggacggg agccaagctc gaccgcgact acttcaaccg
     4921 ggccttctgc cgcatcttct tcggaactgc cgatgactcc atcgcgcgcg ccatcctgct
     4981 cgtggggaac atcaacgggc agaagctgaa gctgcgaaac gcggcggggg gcatcgacga
     5041 gccggcctac accggcaaca gcactttcgg gcgctactac tacgagttct tcggtgaccc
     5101 tttcaccgat cccaaggtac tggacattgt agagcccgga cgcacagggc aggggatcct
     5161 gcaacccgcg ctcgaagcgg agaagctctt ggagcaagcc cggggacagg tgaggcaaaa
     5221 ccaagacgtg ctggagcggc tgctcttcac gagtcgcagc tacgaagcga tgggcaggaa
     5281 gctgatcgcc cggagccatt tcctggacaa caatgttccg cgtgctcagg tcgcggaaga
     5341 actgctttcg ctggccaaaa cttacgaggg cctcaaggct gacttccaac ggctatggct
     5401 ggcggactgc caggacgccg gatcgtttcg cggctacatc cagcgcttcg acaatacgat
     5461 cgtgccgtgc cgcaagaaag cagaggagtt gacccgcatg tcttccacta aggtagccgc
     5521 cgacgtcagt cggcgcattc ttgctgaagc gaagacagcg cggactgacg tcggcggcta
     5581 ccccctggcg gcagatcctg cgacgggtga gatatccaaa ctaccccgta aggaaaaggc
     5641 cgtgctggtg ggcgtgaatt acttccccgg ctggtggagg ccgcacccga acaagtggca
     5701 agaccggcgt ggcgaggatt ggcgattgcg cttcccggaa cgggtgccgt tgctggggga
     5761 gtataacgag caggaaacca tgaaccgcga gatcgcggct gctgccgctg cgggcgtgga
     5821 tttcttctcc atcctctggt attacaatcc gcccgggcat gagcgtgaac ccaatgtggc
     5881 gctaaccgag agcggcctgg ccaacttcat ggcttcgccc gaggccaggc ggctgcggtt
     5941 catgatcacc cgcgcagcgt tgagtacatg cggcgcgcaa aaagcaaatt cctgagcgcc
     6001 aaggtgaagt c
//
LOCUS       DATCGS010000161         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_164285, whole genome shotgun sequence.
ACCESSION   DATCGS010000161 DATCGS010000000
VERSION     DATCGS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164285"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1025)
                     /gene="cysD"
                     /locus_tag="VIL39_03175"
     CDS             complement(123..1025)
                     /gene="cysD"
                     /locus_tag="VIL39_03175"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5480458.1"
                     /translation="MNPRLTHLEALEAESIHIFRGVAAEFAKPVMLYSIGKDSSVMLR
                     LAQKAFHPGKIPFPLLHVDTSRKFREMIEFRDLYTREIGATLIVHRHAQALAEGVNPF
                     QLGTQKCCALLKTRALLDAVAEGGFDAAFGGARREEEKSRAKERIYSFRDAAGQWDPR
                     NQRPELWNLFNGRIDKGESIRVFPLSNWTELDVWHYIHLERIPVVPLYFAKRREMLAR
                     GNTLIPVEHEVPLLPGEKPQWVMCRMRSLGCTPCTGAVRSEADTVPKIIAELVGFRRS
                     ERENRVIDHDQEGSMELKKREGYF"
     gene            complement(1022..2098)
                     /locus_tag="VIL39_03180"
     CDS             complement(1022..2098)
                     /locus_tag="VIL39_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY5480459.1"
                     /translation="MAIEVRHINKTFGSFAALRDVSLNVNDGELVALLGPSGSGKTTL
                     LRIIAGLETPDPDPHGAVLFEGRDVARQDTGERRVGFVFQHYALFRNMTVFENIAFGL
                     RVRPRKMRSSEKQIRDRVTELLKLIHLESLADRYPSQLSGGQRQRVALARALAVEPKV
                     LLLDEPFGALDAKVRQGLRRWLRRLHDEMHVTSLLVTHDQEEALEVADRVVVMNQARI
                     EQVGTPEEVFHHPASEFVMDFMGNVNLFRGRIESGKAVFGPLVLDSPKATGADGQAAR
                     LFVRPYDLDIHHRHNGRPGFGARVLRVQSAGPQVRVELLAETGEAVTVEMPHERFRAT
                     QVSPHDHVYVSMRDARIFTEDYSI"
     gene            complement(2100..2957)
                     /gene="cysW"
                     /locus_tag="VIL39_03185"
     CDS             complement(2100..2957)
                     /gene="cysW"
                     /locus_tag="VIL39_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY5480460.1"
                     /translation="MSAGKGEPVLLRWTLIALALGFMSLFVVLPLAVVFAQAFEKGWA
                     TYWAAVSEPDALAAIRLTLLAAGIAVPLNLVFGLAAAWAIAKFQFPGRNLLVTLIDLP
                     FAVSPVISGLIYVLMFGAQGWLGPWLDQHDIRIIFAVPGIVLATVFVTFPFVARELIP
                     LMEAQGADEEEAALVLGAGRWQLFFRITLPNIKWSLLYGVILCTARAMGEFGAVSVVS
                     GHIRGETNTMPLHVEILYNEYNFAAAFAVASLLAILALVTLVLKRLLEGKIRQDSGYV
                     VAAVEGSEV"
     gene            complement(2954..>3521)
                     /gene="cysT"
                     /locus_tag="VIL39_03190"
     CDS             complement(2954..>3521)
                     /gene="cysT"
                     /locus_tag="VIL39_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY5480461.1"
                     /translation="FPGKRLVDALVDLPFALPTAVAGIALTALYAENGWIGRILVHWG
                     VRVAFTRLGVLVALVFIGLPFVVRSVQPVLQDVDREVEEAAASLGAGRWQVFSRVVFP
                     LVRPALLTGFAMSFARALGEYGSVVFISGNMPMRTEITPLLIITKLEQYDYAGATAIA
                     VVMLVASFLMLLAINLLHWHSRKYAEVR"
BASE COUNT          694 a         1173 c         1039 g          615 t
ORIGIN      
        1 cagcgtggat ttcccgtcat ccacacttcc cgcggtcgtg aaccgcagca ggcccttttc
       61 ctggtcctgc tcgaggaaag cgtctatgtc gtgaccgatc gatgctgctg tcgcggcggg
      121 catcagaagt acccctcgcg tttcttcagt tccatagagc cctcctggtc gtgatcaatg
      181 acgcggttct cccgttccga gcggcgaaac cctaccagct ccgcgatgat cttcggcacc
      241 gtgtccgcct ccgagcggac ggccccggtg cagggggtgc agccgagcga gcgcatgcgg
      301 cacatcaccc attgcggctt ctcgcccggc aggagcggaa cctcgtgctc taccggaatg
      361 agcgtgttgc cgcgcgcgag catctcgcgg cgcttggcaa agtacagagg gaccaccggg
      421 atccgttcca gatggatgta atgccagacg tccagctccg tccagttcga gagcgggaac
      481 actcgaatac tctcgccctt gtcgatccgg ccgttgaaca ggttccacaa ctctggtctc
      541 tggtttcttg gatcccactg gccggcagcg tcgcggaaag agtagatgcg ctctttggcc
      601 cgcgatttct cttcttcacg ccgcgcccct ccgaatgccg cgtcgaaccc accctcagcc
      661 accgcatcca gcagggcgcg cgtcttcaga agagcgcagc acttctgcgt gcccaactga
      721 aatgggttga ccccttcggc cagtgcctgc gcatgcctgt gtacgatgag agtggctccg
      781 atttcccgtg tatagaggtc ccggaattcg atcatttccc gaaacttgcg gctggtgtcg
      841 acgtgcaaca gcgggaaggg aatcttgcca gggtggaagg ccttctgcgc gaggcgtagc
      901 atgaccgacg aatccttgcc gatcgaatag agcatcaccg gcttggcgaa ctccgccgcc
      961 actccccgga agatgtggat gctctccgcc tccagcgctt cgagatgtgt cagtcgcggg
     1021 ttcaaatgga atagtcctcc gtaaaaatcc gggcatcgcg catgctgacg taaacatgat
     1081 cgtgggggct gacctgggta gcccggaagc gctcgtgggg catctcgacg gtgactgcct
     1141 ctccggtttc ggccagcaat tccaccctca cctgaggtcc cgccgactgt actcgcagca
     1201 cgcgcgcacc gaagcctggt cttccgttgt gccgatggtg aatgtccagg tcgtatggcc
     1261 tcacgaacag gcgcgccgcc tggccgtccg cgccggtggc cttgggggaa tccagtacca
     1321 gcggcccgaa cacggccttg ccgctctcga tcctgccgcg aaacaggttc acgttgccca
     1381 tgaagtccat cacgaattcc gaggccggat gatggaagac ctcctccggg gttcccacct
     1441 gctcaatgcg ggcctggttc atcacgacca cgcgatccgc cacttccaag gcttcctctt
     1501 ggtcgtgggt caccagcagg ctggttacgt gcatttcgtc gtgcagccgc cgcagccagc
     1561 gccgcaggcc ctgccggacc ttggcgtcca acgcgccaaa cggctcgtcg agcagcaaca
     1621 ccttgggttc gaccgcgagc gccctcgcca gcgcaacccg ctgccgctga ccgccggaga
     1681 gctgcgaagg gtagcggtcg gccaggctct ctagatgaat cagcttcaac aactccgtca
     1741 cgcggtcgcg aatctgcttc tcgctggacc gcattttgcg cggacgcact cgcaggccga
     1801 aggcgatatt ctcgaagacg gtcatgttcc ggaacagcgc atagtgctga aacacgaaac
     1861 ccacccgccg ctcgccggtg tcctggcgtg cgacgtcgcg gccttcgaac aacaccgcgc
     1921 cgtgggggtc gggatcgggg gtttccagac cggcaatgat ccgcaacagg gtggtcttgc
     1981 ccgacccgga tggacctaat agcgcaacca gttcgccgtc gttcacattg aggctcacgt
     2041 cgcgcagagc ggcgaagctg ccgaaggtct tattgatatg gcgtacttcg atggccacgc
     2101 tatacctccg agccttccac tgccgcgacg acgtatccgg agtcctgccg gatcttaccc
     2161 tccagcaggc gcttgagaac cagtgtcacc agggccagga tcgccagcag gctggccacg
     2221 gcgaaggccg cggcgaagtt gtactcgtta taaaggattt ccacatgcag gggcattgtg
     2281 ttggtctctc cccggatgtg ccccgacacc acggagacgg ccccaaactc ccccatcgcc
     2341 cgcgccgtgc acaggatgac gccatacagc aggctccact tgatattggg cagggtgatc
     2401 cgaaagaaga gttgccagcg ccccgctccg agcaccagag cggcctcttc ctcgtcggcg
     2461 ccttgcgcct ccatcagagg gatgagttcc cgcgcgacga agggaaatgt cacgaagacc
     2521 gtggccagaa cgatgcccgg cacggcgaag atgatccgga tgtcgtgctg gtccagccac
     2581 ggccccagcc aaccctgcgc tccgaacatc agcacataga tcaatccgga aatcaccggc
     2641 gagaccgcga atggcaggtc gatcaaagtc accagaaggt ttctgcccgg aaactgaaac
     2701 ttggcgatgg cccatgctgc agccaggccg aaaaccaggt tcaagggaac cgcgattccg
     2761 gcggccagca gcgtgagccg gatcgccgcg agtgcatccg gttcactgac agccgcccaa
     2821 tacgtcgccc aacctttttc gaacgcctgc gcaaagacca ccgccagcgg caggacgacg
     2881 aagagcgaca tgaagccgag cgccagggcg atcagcgtcc accgcagcag gaccggttcg
     2941 cctttgcccg cgctcatctg acctccgcgt acttacggct gtgccaatgc aagaggttga
     3001 tggccagcag catcaggaaa gaagccacca gcatcacgac ggcgatggcc gtggccccgg
     3061 catagtcgta ctgctccagc ttggtgatga tcaacagggg ggtgatctcc gtgcgcatcg
     3121 gcatgttgcc ggagataaag acgacggagc cgtactctcc cagcgcccgg gcgaaggaca
     3181 tcgcaaaacc tgtcaacaga gccgggcgca ccaacgggaa aactacgcgg ctgaatacct
     3241 gccagcggcc cgcacccagg ctcgcggcgg cttcctcgac ttcccggtcg acgtcctgca
     3301 ggacgggctg tacgctccgc accacaaacg ggagaccgat gaagaccaac gccacaagca
     3361 cgcccagccg ggtgaaggcc acccgtaccc cccaatgcac cagaatccgt ccaatccagc
     3421 cgttctcggc gtaaagggcg gtcagtgcga tccccgctac cgcggttggc aacgcaaacg
     3481 ggagatccac cagggcatcc accagacgct tcccgggaaa c
//
LOCUS       DATCGS010000162         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_411165, whole genome shotgun sequence.
ACCESSION   DATCGS010000162 DATCGS010000000
VERSION     DATCGS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411165"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VIL39_03195"
     CDS             <1..238
                     /locus_tag="VIL39_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480462.1"
                     /translation="FFVIALISSGGNVPALAIFAVVGVATGITFGVLLPFLVLSFANG
                     FYRERLKGLLHLGGAAAPPVMTPPMPVAAAADGS"
     gene            433..1614
                     /locus_tag="VIL39_03200"
     CDS             433..1614
                     /locus_tag="VIL39_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480463.1"
                     /translation="MKQLTRTVKWVALAAMALATTALAQKEQWLNYHVSTEGRGYRYL
                     NLTTNPPPNIKLPKLSGQAYFAQWTTPLDPAGRWLCLDRTKKSGLYDRVYFDSTGNGR
                     LDDKTPVGTSRSDQYTAYFEPVRVVFKGEDGPITYHLILRFMQYGAGEVNLLASSGGY
                     YAGKVDIGGKKRSIELIDGNVNGTFNDRSADMSDSDRVMVEGDKVGERYLGKMIEVDG
                     QFYQIEVARDGAFIKLQKAENVVLGQVRVPETITEFVAFGENGHFVRKPVKGGFTLPV
                     GKYRIQDWKIERKDAKGAAWELSAYNFNDSARFEVADAKPVSLEVGEPMRAVMEAQEP
                     SRQLGSREVTNQLTFSLRFQGHYGETLQIMKGNQRPPGPRLTLTSLDGTYRYTNTFEF
                     G"
     gene            1717..>2679
                     /locus_tag="VIL39_03205"
     CDS             1717..>2679
                     /locus_tag="VIL39_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480464.1"
                     /translation="MTLKPSGRKPPELGPKVRVEAAHLSPAGDAADLAPLINGAGLRD
                     SDKDGLLEQDTAAKNMWRGACATNLSLEFELPEPVALGTIEVWNYNAEWQMADGVRKA
                     DVAVSPDGTTWETVLRGAEFAEAEGNVDYDEPIQLKLKGATVRKVRFENIVPRSDKGM
                     VGLSKVVFHQAMGAQAGPRMPEDGATGIGIGKLALDWVAGQGATEHRVYLGTAADNLA
                     LAGTTKTTRMDAPQLKPDTTYFWRVDEAQGDGRVVTGRVARFGTSGLVAWWKLDETKG
                     SKAEDATGHQFTGNVVGRSNWALEQGRRGGALDFDGRTTFINCGR"
BASE COUNT          570 a          716 c          924 g          469 t
ORIGIN      
        1 cttcttcgtc attgcgctga tttcgagcgg gggcaacgtt ccggcgctgg cgatatttgc
       61 agtcgtgggg gtggcgaccg ggatcacgtt tggcgtgctg ctgccgtttc tggtgctgtc
      121 ctttgccaat ggcttctacc gggagcggct gaagggttta ttgcacttgg ggggcgcggc
      181 ggcgccaccg gtgatgacgc cgccaatgcc ggtcgcggct gcggcagacg gaagctgaac
      241 ggcgattggc agcagagaac cgaaacaaat ccgaatgacg ggggttaata atcctggtga
      301 tgggttgaac agacgagggg gcaggcaggt cataagagtg ttggtgaatg gcgaggcaca
      361 gtgataaatg gtgctgtcat cagtgtgcgc agtgtgctag aagcgcattg gagttaatcg
      421 aaaggctcgt ttatgaagca acttacgagg acggttaagt gggtggccct ggcagcgatg
      481 gcgctggcaa cgacagcctt ggctcagaag gagcaatggc tgaactacca tgtgagcacc
      541 gagggacggg gttatcgcta cttgaatctc accaccaacc cgccgcccaa catcaaactg
      601 cccaagctta gcgggcaggc ctatttcgcc cagtggacca cgccgctgga cccggcgggc
      661 cggtggctct gcctggaccg cacaaagaag tccgggctgt atgaccgtgt ttattttgac
      721 agcaccggca acgggcgact ggatgacaag acgccggtcg gcacctcgcg cagcgatcaa
      781 tacaccgcct atttcgagcc cgtccgcgtg gtgttcaaag gcgaagatgg accgatcaca
      841 tatcacctta tattgcgctt tatgcaatat ggggcaggcg aggtgaactt gctggcctcg
      901 tcgggcggat actacgcggg caaggtggac atcggcggca agaagcggag catcgagctc
      961 atcgatggca acgtcaacgg tacattcaat gaccggtcgg cggacatgag cgactccgac
     1021 cgcgtgatgg tggagggaga caaggtcggc gagcgctacc tgggcaagat gatcgaggtg
     1081 gacgggcagt tttaccaaat cgaagtcgcc cgcgacgggg cgtttatcaa gctgcagaag
     1141 gcggagaacg tcgtgctggg ccaagtgcgc gtgccggaga ccatcacaga atttgtggcc
     1201 ttcggtgaga acggccattt cgtgcggaag ccggtgaaag gtggcttcac gctgccggtc
     1261 ggcaaatacc gcatccagga ttggaagatt gagcgcaagg acgcgaaagg ggccgcctgg
     1321 gagctgagcg cctacaactt caatgattcc gcccgcttcg aggtcgcgga cgccaaacca
     1381 gtgtcgctgg aggtcgggga gccgatgcgt gccgtgatgg aagcccagga gccgagcaga
     1441 cagcttggtt cccgagaggt aaccaaccaa ctcactttca gtctccgctt tcagggtcat
     1501 tacggcgaga cgctgcaaat catgaagggc aaccagcggc cacccggccc gcggctgacc
     1561 ctcaccagcc tggacggcac ttaccgttac accaacacct tcgagtttgg gtgaggtggc
     1621 gtatgttcgc tctcatggcg agagcccaaa acggtcaaac gtcctgtcat tgcaaccgtc
     1681 ggctttgcca gccccttcca ggtgctgaca cgccagttga cgctaaagcc cagcggccgc
     1741 aagccgcctg agctgggtcc caaggtgcgc gttgaagccg cgcatctaag cccggcaggc
     1801 gacgcagcgg atctggcgcc gctcatcaat ggagccggat tgcgcgactc ggacaaggac
     1861 ggtttgctgg aacaggacac cgccgcgaag aatatgtggc ggggcgcttg cgccacgaac
     1921 ctgagccttg agttcgagct gcctgagccg gtggcgctgg ggaccatcga ggtgtggaac
     1981 tacaacgccg aatggcaaat ggccgacggc gtgcgcaagg ccgatgtggc cgtgtcgccc
     2041 gatgggacga cctgggaaac agtgttgcgc ggggccgagt tcgccgaggc ggaaggcaac
     2101 gtggattatg acgagccgat ccagttgaag ctcaagggcg caacggtgcg caaagtgcgg
     2161 ttcgaaaaca tcgtgccgcg gagtgacaaa ggcatggtgg gtttgagcaa agtcgtcttc
     2221 caccaagcca tgggggcgca agctggaccg agaatgcccg aagacggcgc gacaggtatt
     2281 ggcattggca agctagcatt ggattgggtc gcggggcagg gggcgacgga gcaccgggtt
     2341 tacctgggaa cggctgccga taacctcgcc ctggccggca cgaccaagac gacgcggatg
     2401 gacgcaccac agttgaagcc cgatacaacc tacttctggc gcgttgatga ggcccaggga
     2461 gacgggcgcg tggtgaccgg gcgggtggcg cgctttggta ccagcgggct ggtcgcgtgg
     2521 tggaagcttg atgagaccaa agggtcgaag gccgaggatg ccacgggaca tcagttcacc
     2581 ggcaatgtgg tcggcaggtc aaattgggca ctggagcagg gccggagggg aggcgcgctg
     2641 gacttcgacg gcaggacaac gttcatcaac tgcggcagg
//
LOCUS       DATCGS010000163         6634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_64910, whole genome shotgun sequence.
ACCESSION   DATCGS010000163 DATCGS010000000
VERSION     DATCGS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64910"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VIL39_03210"
     CDS             <1..541
                     /locus_tag="VIL39_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5480465.1"
                     /translation="TRNWPHQLYVREARRMVSDYVMTEHNCRGQQQAEDPVGLGAYGM
                     DSHNCQRIVKNGHAENEGDVQVHGFSPYPISYRSIVPKAAECENLLVPLCLSATHIAY
                     GSIRMEPVFMILGQSAATAAALAMDGKSTVQKLPYETLRARLAADKQVLEWKAPQSGR
                     KAPKAGPGVANPKPETRKP"
     gene            538..1275
                     /locus_tag="VIL39_03215"
     CDS             538..1275
                     /locus_tag="VIL39_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY5480466.1"
                     /translation="MSTQPVHLSPEAQATLPRHVAIIMDGNGRWAKQRHLPRIEGHRA
                     GAESARVIIRTAGELGIKYLTLYAFSVENWNRPKDEVDALMKYLVHYLKTETPELNKN
                     NVRLEVIGQIYRLPEAVQEHLKKSIATLSKNNGLTLVMALSYGGRTEIVDAVRSIATK
                     VRAGELDPPDITEKLFAQHLYTRNLPDPDVLIRTSGEMRVSNFLLWQISYTELVVTQT
                     LWPDFRKPQLYEALEEYNRRHRRFGGL"
     gene            1362..2285
                     /locus_tag="VIL39_03220"
     CDS             1362..2285
                     /locus_tag="VIL39_03220"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5480467.1"
                     /translation="MPNATGSTPPPLSKAKVFLRRLISFVVLWTIVLTAMFSHNRLVS
                     DSVFLLIMVLLAALGLAEFYGLVARRGWICFKRWGILGGVLLMAGTFLNLTGKLGTSG
                     SPARVNDFETSFLILFVLGLCLCQFFSRGNTAGLLAISMTLFGLMYVPWLLNFIQKIN
                     FFFYPGLAGRYYLLYFLLVTKFSDSGAYAIGSLIGRHKMIPRISPGKTWEGFGGAILG
                     STLASLVFVYFLGPEMPGMKWKHAVILGGLLGISAVVGDLIESLFKREAGAKDSGRFF
                     PGIGGMLDLLDSLLFNAPIMYLYLRHVLTKP"
     gene            2299..3438
                     /locus_tag="VIL39_03225"
     CDS             2299..3438
                     /locus_tag="VIL39_03225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5480468.1"
                     /translation="MRNVVLLGSTGSIGTSTIKVAEDLPDRIRLLGLAAGNNGELLLE
                     QTRKHRPEAVSITDPRKARELRDTLGTSTEVLSGPEGLLKLATLPGADIVLIAIVGTA
                     GLQPALAAIRAGKDIAVASKEILVMAGEIVMREAREHGVKVLTVDSEHSAIFQCLDGK
                     PAESIRRLWLTASGGPFRTTPKEEFAAITVERALKHPSWVMGRKITIDSATLFNKGLE
                     MIEARWLFDVEMGRVSVVVHPQSVVHSLVEFIDGSMLAQLSTPDMCLPIQYALTYPDR
                     APSQRVQTNLAKLGTLTFEEPDPDRFPALDLARRAGEVGGTLPAVLNAANEVAVDAFV
                     NRRISFLDISALVRRTMERHQTVPHPTLQQILEADTWARAEAARS"
     gene            3605..4987
                     /gene="rseP"
                     /locus_tag="VIL39_03230"
     CDS             3605..4987
                     /gene="rseP"
                     /locus_tag="VIL39_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY5480469.1"
                     /translation="MNIVYVVLAVLLLFGAAVFVHEYGHYWMALRRGLKIEGFSIGFG
                     PKIMTWMHDGVEWSWRWIPAGGFVKLPQMATSDALEGKADQPADQLPSASPWSKILVA
                     FSGPFMNVVFGIAIAFLLWGTGIPVPVNPPIIGYVDPDSPEAKLGIQPGDEIRSVDGK
                     PITSWRDVQETSILARSTNLSVVIARPGKFTNTYELTTVVSESLGLKILNLDPRDHPV
                     IKRVLSDSAAEAAGLKAGDEVLVFAQIPLHGADQFIKLIQKCPGEAKDIVIKRDGARK
                     TLKVTPRMDSSAKVGRIGAEIGTGRPIYRIEHIPPWTQIGDVMDRTIGTFSALIHSKQ
                     TGVGLKDLSGPPGILALLAAYVNSDWRLALSFLVLLNINLAIINLFPMPVLDGGHIMM
                     ALVERLFGRPLPNRLVEYTSTIFAVLLIGFMLYVSFNDVRRFSLFRSMFKQEVTIEKQ
                     ESAPAPAGGR"
     gene            4990..>6634
                     /gene="ispG"
                     /locus_tag="VIL39_03235"
     CDS             4990..>6634
                     /gene="ispG"
                     /locus_tag="VIL39_03235"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY5480470.1"
                     /translation="MLSYCESPYYLLRRRTREVIVGDPQRGGVIIGGDHPVVMQSMIT
                     CDTMDTAECVKQTLELVAVGCQIVRITAPTVKDAANLKSIVAELRARGCHVPIVADIH
                     FKPEAALEAARWVEMVRINPGNYADSKKFAIKEYADDQYAAELARIEEKFTPLVKLCG
                     ELGRALRIGTNHGSLSDRIMNRFGDSPLGMIESALEFARIARKHDFHNFKFSMKSSNP
                     KIMIQCYRLLVARLEQEGPDWNYPIHLGVTEAGEGEDGRIKSAIGIGSLLCDGLGDTI
                     RVSLTEDSPREIEVCRDLLAQVPALSAECGMRNADFPANWPFDPFAYTRRAAPEIELA
                     EGAKCGGEQTVRVVVTRATWNKVAPKLSPKEDVKPEAVYEALNICEVDPTRAFEVNCD
                     TQLVTVKDGASLPAIAAFRLLAAQLKRLGRNNPILLKDCLGFRPAPLEPKIALLRAAA
                     VIGSLLADGIGDAILIRGEAGAGQSLRLAYNILQAAGCRSFKTDYVACPSCGRTLFNL
                     QTVTGRIKARTEHLKGVKIAIMGCIVNGPGEMADADFGYV"
BASE COUNT         1265 a         2025 c         2052 g         1292 t
ORIGIN      
        1 cacgcgcaac tggccgcacc agctctacgt gcgcgaagcc cgccggatgg tgtcggatta
       61 cgtcatgacc gagcacaact gccgcggcca gcaacaggcc gaagatcccg tggggctggg
      121 cgcctacggg atggactcgc acaactgtca gcgcattgtt aagaacggtc atgcggaaaa
      181 cgaaggcgac gtgcaggtgc acggcttctc gccctacccc atctcctatc gctcgattgt
      241 gccgaaggcc gccgagtgcg agaacctgct ggtgccgctt tgcctgtccg cgacgcacat
      301 tgcctacggc tcgattcgca tggagccggt gttcatgatc ctcggccaat cggcggccac
      361 cgccgccgcg ctggccatgg acggcaagtc caccgtgcag aagctcccgt acgaaaccct
      421 ccgtgctcgc ctggccgctg ataagcaggt gctggagtgg aaagcgccgc aatcaggcag
      481 gaaggctccc aaggcgggcc cgggcgttgc aaatccgaaa cccgaaaccc gaaaaccatg
      541 agcacgcagc ccgtccatct cagccccgaa gcccaggcca ccctgccgcg gcatgtggcg
      601 atcatcatgg acggcaatgg ccgatgggcc aagcagcggc acctgccgcg catcgagggc
      661 catcgcgcgg gggcggaatc ggcgcgcgtc atcatccgaa cggcgggcga actggggatc
      721 aagtatctga cgctctatgc gttctccgtc gagaactgga accggcccaa ggacgaagtg
      781 gatgcgctga tgaagtatct ggtgcattac ctcaagaccg agacgcccga gctgaacaag
      841 aacaacgttc ggctggaagt catcggccag atctatcgcc tgccggaggc ggtgcaggag
      901 cacctcaaga agtccatcgc caccctctcg aagaacaacg ggctgacctt ggtcatggcg
      961 ctcagttacg gcggacggac ggagatcgtg gatgcggtcc ggagcattgc cacaaaggtg
     1021 agggcgggcg agcttgaccc gcccgatatc acggaaaagc tgttcgcgca gcacctctac
     1081 acgcgcaacc tgccggaccc cgatgtgctg atccggacca gcggagagat gcgggtgagc
     1141 aacttcctgt tatggcagat ttcctacacg gaactggtgg taacgcagac gctgtggccg
     1201 gacttccgca agccgcaact ctatgaggcc ctcgaagaat acaatcggcg gcaccgcagg
     1261 tttggggggc tgtgatttac gatttgcggg cgcagggaat gccgcgcatg aaccgcaaat
     1321 cctctcgaca tccatttcct gctcgttaac ctcccctcac catgcctaac gcaaccgggt
     1381 caacaccgcc gcccctatcg aaggccaaag tctttttgcg ccggctgatc agctttgtgg
     1441 tgctctggac gattgtcctg acggcgatgt tctcccacaa ccgtctggta tcggactccg
     1501 tgttcctgct gatcatggtc ctcctggcgg ccctcgggct ggcggagttt tacggcctgg
     1561 tcgccaggcg cggttggatc tgcttcaaac gctggggcat tctgggcggc gtcctgctga
     1621 tggcgggaac gttcctgaac ctgaccggga agttggggac ctccggctcc ccggcgcgcg
     1681 tcaacgactt cgaaaccagc ttcctcattc tcttcgtgct gggcttgtgc ctgtgccaat
     1741 tcttctcgcg cggcaacacc gcgggcctgc tggccatttc gatgacgctc ttcggcttga
     1801 tgtatgtgcc ctggctgctg aacttcatcc agaagatcaa tttcttcttc tatcccggcc
     1861 tcgcggggcg ctactacctg ctttacttct tgctggtcac caaattcagc gacagcggcg
     1921 cgtatgccat cggatcgctg atcgggcggc acaagatgat cccgcgcatc agcccgggca
     1981 agacttggga aggctttggg ggggcgatcc tgggttcgac gctggccagc ctggtgtttg
     2041 tgtatttcct ggggccggag atgcccggca tgaaatggaa gcacgcggtg atcctggggg
     2101 gcctcctggg catttctgcc gtcgtagggg acttgatcga atccctcttc aagcgtgaag
     2161 cgggggcgaa ggattcgggc cggttcttcc ccggcattgg cggcatgctc gacttgctgg
     2221 acagcttgtt gtttaatgcg cctatcatgt acctgtactt aaggcatgtg ctgacgaagc
     2281 cttaagcgta aatcgtatat gcgtaatgtc gttctgttag gcagcaccgg ttccataggc
     2341 accagcacca tcaaggtggc ggaagacctg ccggatagga tccgtctgct tgggctggcg
     2401 gcgggcaaca atggggaatt gctcctggag cagacccgca agcatcggcc cgaggccgtt
     2461 tccatcaccg acccgaggaa ggcgcgggaa ttgcgcgata ctctgggcac gagcacggag
     2521 gttctttccg gccctgaggg cctgttgaag ctcgctacgc tgcccggggc ggatattgtg
     2581 ctgatcgcca tcgtgggcac cgccggtttg cagccggcgc tggccgccat ccgagcgggt
     2641 aaagatattg ccgtggcgtc caaggaaatc ctggtcatgg cgggcgaaat cgtcatgcgc
     2701 gaagcccgcg agcatggtgt gaaggtgctg accgtggaca gcgagcactc ggcgatattc
     2761 cagtgtctgg acggcaagcc agccgagtcc attcgccggc tttggctcac ggcatccgga
     2821 ggccccttcc gcactacgcc caaagaggag tttgcggcta tcacggtcga gcgcgcgttg
     2881 aagcatccgt cctgggtaat gggccgcaag atcaccattg attcggccac gctcttcaac
     2941 aaagggctgg agatgattga agcgcgctgg ctgttcgacg tggaaatggg ccgcgtgtca
     3001 gtggtggtcc atccgcagag cgtggtgcat tcgctggtgg agttcataga tggctcgatg
     3061 ctggcgcagc tttcgacgcc ggacatgtgc ctgcccatcc aatatgccct gacctaccct
     3121 gaccgggcgc ccagtcaacg cgtgcaaacc aacctggcca aactgggtac gctgaccttt
     3181 gaggaacccg acccagaccg gttcccggcg ctggacctcg cacgccgggc cggcgaagtg
     3241 ggtggcacgc tgccggcggt gctaaacgcg gctaatgagg tcgcagtgga tgcctttgta
     3301 aaccggcgga tcagctttct ggacatctcc gcccttgtgc ggcgcaccat ggagcgccat
     3361 cagacggtgc cgcaccccac gttgcagcag attctcgaag cggacacttg ggcgcgggcc
     3421 gaagccgccc ggagctgata gccgagagcc ggcgcatggg gacgccggcc tgggtgaagg
     3481 ggttcgagga aggtcacggt aatgcaggcg tgttgccatt ccgcatcctc ccttcgacca
     3541 ctccaacaaa taactcgcat aagcgcccag tccggttaac ttcttcctac caaatatgtg
     3601 gttgttgaat attgtttacg tggtgctcgc ggtcctcttg ctcttcggag ccgcggtgtt
     3661 tgtccacgag tatgggcatt attggatggc gctgcgccgc ggcctgaaga tcgagggctt
     3721 ctcgatcggg tttgggccga aaatcatgac ttggatgcac gacggcgtgg aatggtcgtg
     3781 gcgctggatt ccggcagggg gcttcgtgaa actgccccag atggccacgt ccgacgcact
     3841 ggaaggcaag gccgatcaac ctgcggacca actgccctcg gcttcgccct ggtcgaagat
     3901 cctggtcgcc ttttccgggc cgttcatgaa cgtggtgttt gggattgcca ttgcgttcct
     3961 tctgtggggc actgggattc ccgttccagt caatccgcct attatcggct acgttgaccc
     4021 ggactcgccg gaggcgaagc tcggcattca gccgggtgac gaaatccggt cagtggacgg
     4081 caagccgatc acttcgtggc gagacgtgca ggagacgagt attctggcgc gcagcaccaa
     4141 cctgtcagtg gtcatcgcgc gtcccggaaa gttcaccaac acctatgagc tgacgaccgt
     4201 cgtgagcgag tctctcggcc tcaagattct taatctcgac ccgcgcgatc atccggtcat
     4261 taaacgcgtc ctgtccgata gcgcggcgga ggcggcgggg ctgaaggcag gcgacgaagt
     4321 cctggtattc gcccaaatcc cgctgcacgg cgccgaccag ttcatcaagc tcatccagaa
     4381 gtgccccggg gaggcaaaag acatcgtcat caagcgcgac ggcgcacgca agacactgaa
     4441 agtcaccccg cgcatggact ccagcgccaa ggtcggtcgc atcggcgccg agatcggcac
     4501 gggcaggccg atttatcgga tcgaacacat tccgccctgg acccagatcg gcgacgtcat
     4561 ggaccgcacc attggcactt tcagtgccct gattcactcg aaacagactg gcgtcgggtt
     4621 gaaggatctg agcggcccac cgggcattct ggcgctgctg gccgcctacg tgaactcgga
     4681 ttggcggctg gcgttgagct tccttgtgct gctcaacatc aacctggcca tcatcaacct
     4741 cttccccatg cccgtgctgg acggcggcca catcatgatg gcgctcgtgg aacggctatt
     4801 cggccggccc ctgcctaatc gcctggtgga atacacctcc accatctttg ccgtcctgct
     4861 catcggcttc atgctgtatg tctcgtttaa cgacgtgcgc cgcttctcgc ttttccgctc
     4921 catgttcaag caggaggtca cgatcgagaa gcaggagagt gcgcccgcac ccgccggtgg
     4981 ccgctgaaca tgctcagtta ctgcgaatct ccttactatt tgctccgccg ccgcacccgc
     5041 gaagtgatcg tcggcgaccc ccaacgagga ggggtcatca ttggcggcga ccatcccgtg
     5101 gtgatgcagt ccatgatcac ctgcgacacg atggacactg ccgagtgcgt gaagcaaacg
     5161 ctggagctgg tggccgtcgg ctgccagatc gtgcgcatca cggcgccaac ggtgaaggac
     5221 gccgccaacc tgaagagcat tgtggccgag ctccgcgcgc gcgggtgcca cgtgccgatt
     5281 gtagccgaca tccatttcaa gccggaagcc gccctcgaag cggccagatg ggtggaaatg
     5341 gtccgcatca accccggcaa ttacgccgac tccaagaagt tcgccattaa ggagtatgcg
     5401 gacgaccagt acgctgccga attggcgcgc atcgaggaga agttcactcc gctggtgaag
     5461 ctgtgcgggg aactgggccg cgccctgcgc atcggcacca atcatggttc tctgagcgac
     5521 cgcatcatga accgcttcgg ggactctccg ctcggcatga tcgagagcgc gctggagttc
     5581 gcccgcatcg cccgcaagca cgacttccat aacttcaagt tctcgatgaa gtcgagcaac
     5641 cccaagatca tgatccagtg ctatcggttg ctcgtggcgc ggttggagca ggaaggaccc
     5701 gactggaatt atccgattca cctgggcgtc accgaagcgg gtgaaggcga ggacggacgc
     5761 atcaagagcg ccatcggcat tggatcgctg ctgtgcgatg gattaggcga caccattcgg
     5821 gtttccctga cggaggattc gccgcgcgaa atcgaggtct gccgcgactt gctggcgcag
     5881 gttcccgctc tcagtgcgga atgcggaatg cggaatgcgg atttcccggc caactggccc
     5941 ttcgatccgt tcgcttacac gcgccgcgcc gcgcctgaga tagagcttgc tgagggcgcg
     6001 aaatgcggcg gggaacagac tgtgcgggtg gtggtgacgc gcgccacctg gaacaaagtc
     6061 gcccccaagc ttagcccgaa agaggatgta aagccggagg ccgtctatga agccctgaat
     6121 atctgtgaag tcgatccgac ccgggccttc gaggtgaact gcgacaccca actggtgacc
     6181 gtgaaggacg gcgcctcgtt accggctatt gcggccttcc gcttgctcgc agcgcaattg
     6241 aaacggctgg gcaggaacaa ccccatcctc ttgaaagact gcctcggctt ccgcccagca
     6301 ccactggagc cgaagattgc tttgctccgc gccgcggcgg tcatcggctc attgctcgcc
     6361 gacggcatcg gcgatgcgat tctgattcgc ggtgaagccg gagccggaca atccttgcgc
     6421 ctggcctaca acatcctcca agcggccggt tgccgctcgt tcaagacgga ttatgtcgcc
     6481 tgcccctcgt gtggacgcac cctcttcaat ctccaaaccg tcaccggccg catcaaagcg
     6541 cgcaccgagc atctcaaggg tgtgaagatt gcgatcatgg gctgcatcgt gaatggtccg
     6601 ggtgagatgg cggatgcgga tttcggttac gtcg
//
LOCUS       DATCGS010000164         3846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_262565, whole genome shotgun sequence.
ACCESSION   DATCGS010000164 DATCGS010000000
VERSION     DATCGS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262565"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VIL39_03240"
     CDS             complement(<1..602)
                     /locus_tag="VIL39_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5480471.1"
                     /translation="MSTDMNADPKTTFAKRLEQARKMRGLSLRALSQEVQGVVTYAAL
                     HKYEKAAMLPGSDVLVALAGALKQSADFFFRPLTVSLQKVEFRKRATMPVKQKEAVKE
                     QARDFFERYLEVEHLLGLDVGFVNPLAGMVIRAPEDIETAAEKLRNKWDLGASALPNV
                     LELLEEHQVKVYDLEAPDKFDGMSGWAGKIPVVVVNRRFPA"
     gene            complement(807..883)
                     /locus_tag="VIL39_03245"
     tRNA            complement(807..883)
                     /locus_tag="VIL39_03245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Met,seq:cat)
     gene            complement(993..1637)
                     /locus_tag="VIL39_03250"
     CDS             complement(993..1637)
                     /locus_tag="VIL39_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY5480472.1"
                     /translation="MSFSEYIFLAASSLFVIVDPLAAVPAFLAMTPTDTPEQRIKMAR
                     LACCVAAGVLLAFAIAGKWIFKFLGITMPAFQLAASVVLLLIALDMLRAQRSRVQETS
                     EETAAGAEKTDIAITPLAIPMLSGPGAISTAIMLHNQAAGMGQRVALYLCIGAVCVAS
                     YLILRLSARSARWISPIAMSITVRIMGLLLAAIAIQFTLNALRQLNPALFPTAA"
     gene            complement(1634..2971)
                     /locus_tag="VIL39_03255"
     CDS             complement(1634..2971)
                     /locus_tag="VIL39_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480473.1"
                     /translation="MTSETIPSAPADAVERARQVRERMPAEGLFADHDWRTSPIPYPL
                     GPALAKEVESLGRVLLQFYRAVNLLYRKSVEGKQPEWIARWLDQGKPAELIALQRSPA
                     LKNELPRVIRPDLLVTEHGLSVTELDSVPGGIGLTGWLNQTYSAMGAEVLGGADGMLR
                     GFASIFGDAPTVHIVVSGEAATYRPEMSWMANQLGSSRFKVQDAKFNAFADGDAVYRF
                     FELFDLANVPNARVILDLAAAKRIRLTPPPKPMFEEKLLFALLWNRNLQSFWRQELGE
                     GFFQRLLGLAPYTWIVDPAPLPPHAAIPELNLTDWRQLKTLSHKERELILKVSGFSAH
                     AWGARGVYLGSDLSHADWAAAVDEALANFERSPCVLQRYHKPGLVEAQWFDFERQQLL
                     PMKGRARLCPYYFVAGDGDAARAELGGVLATICPADKKIIHGMKDAVFAPCSL"
     gene            complement(3010..>3846)
                     /locus_tag="VIL39_03260"
     CDS             complement(3010..>3846)
                     /locus_tag="VIL39_03260"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY5480474.1"
                     /translation="KEALNSNLAIARIMIEAGRRTLPAFPEHAIQVVPTTDREAIKEL
                     LSLTQYVDLCMPRGGEGLIRAVAECSKVPVIKHYKGVCHVYVDADADLKMAEAITLNA
                     KVQRPAVCNAMETLLVDRKVAGVFLPAIAQRLGEKKVQLRVDAEAETILNSALRTPYS
                     ALKRATEQDWFTEYNDYILNVRVVDGVRQAIDHINHYGSAHSDSIVTRDEGRAKQFLG
                     EVDSATVYWNASTRFTDGGEFGMGAEIGISTDKIGARGPMGLEELTSYKWLGIGSGQV
                     RT"
BASE COUNT          730 a         1203 c         1168 g          745 t
ORIGIN      
        1 gcagggaagc ggcggttcac aaccaccacg ggaatcttgc ccgcccagcc cgacatgccg
       61 tcgaacttgt ccggcgcttc cagatcataa acttttacct gatgttcttc gagcaactcc
      121 aggacgttgg gcagggcgct cgcgcccaaa tcccatttgt tccgcagctt ctccgccgca
      181 gtttcgatgt cctcgggtgc gcgaatgacc atgccggcca gcgggttcac gaaacccacg
      241 tccaatccga gcaaatgctc cacctccaag tagcgctcga aaaagtcgcg tgcttgttcc
      301 ttgaccgctt ccttctgctt cacgggcatg gtggcacgct tgcggaactc aactttttgg
      361 agcgagacgg tcagggggcg gaagaagaag tccgcgctct gcttcagcgc gcccgccagg
      421 gctaccagca cgtcactgcc gggcagcatt gcggcttttt cgtatttgtg aagggcggcg
      481 taggtgacta ctccctgcac ttcctgagac agcgctcgca gggacaggcc gcgcattttg
      541 cgggcctgct ctagtcgttt ggcaaaggtg gttttggggt ccgcattcat atcggttgac
      601 acaatatcgt gttatgtgtc tatttgtcaa cccaagattc tggggattct tgcatcctca
      661 cgcatcggca acgtcgaaca tgaggcgctt gacgtttcgc ccttctgttg aagtcgctgg
      721 gagcagccct tggccgggtg aacagggtaa acgcgcgaaa gcaccaacaa caacgggcct
      781 caaacgcttg tctgaggccc gtgaattggt tgcgggggct ggatttgaac cagcgacctt
      841 caggttatga gcctgacgag ctaccgggct gctccacccc gcgatcccga gaacaaccta
      901 atcatgcctg agcttttctc ctatgcaagc gctattctgg agaatgtgta gaattcctcc
      961 gccaaagaga atcgggagaa ggtcaattga actcaggctg cagttgggaa cagggccgga
     1021 ttgagttgcc ggagagcgtt gagtgtgaac tgaatcgcta ttgccgccag gagcaggccc
     1081 atgatgcgca cggtgatact cattgcaatc gggctgatcc aacgggcgct gcgcgccgaa
     1141 aggcggagga tgagatagct ggccacgcaa acggccccga tgcacagata cagggcgacg
     1201 cgttggccca tgccggctgc ctggttatgg agcatgatgg ccgttgatat ggccccgggg
     1261 ccggacaaca tggggattgc cagcggcgtt atggcgatgt ccgtcttttc ggcgcccgcc
     1321 gccgtctcct cggaggtctc ctgaacgcgg gagcgctgcg ctcgcagcat gtcgagcgcg
     1381 atcaagagca gaaccacgct ggcggcgagt tggaacgcgg gcatggtgat gcccaggaac
     1441 ttgaatatcc acttccccgc aatggcgaaa gccagcagca cgcccgccgc cacgcaacag
     1501 gcaaggcgtg ccatcttgat cctctgttcc ggtgtgtctg tcggcgtcat ggccagaaaa
     1561 gccggcaccg ctgccagcgg gtccacaatc acgaacaggg agctggcggc caggaagatg
     1621 tattcggaga agctcatagc gaacagggcg cgaagaccgc gtctttcatc ccgtgaatga
     1681 ttttcttgtc agccgggcaa atcgtcgcca gcacgccgcc cagttcggcg cgcgccgcgt
     1741 cgccgtcacc ggctacgaag tagtaagggc agagccgcgc gcgccctttc atcggcagaa
     1801 gctgctgccg ctcgaagtca aaccactgcg cctcgaccag gcccggcttg tgataacgct
     1861 gcaggacgca cggtgaccgc tcgaaattgg cgagcgcctc atccaccgcc gccgcccagt
     1921 cggcgtgcga aaggtcgctg cccagataga cgccccgcgc accccacgcg tgcgccgaaa
     1981 agccggacac tttcaggatc aactcgcgtt ccttgtggga cagcgtctta agctgccgcc
     2041 aatccgtaag gttcagctcg ggaatcgcgg cgtgcggggg caggggagcg ggatctacga
     2101 tccaggtgta gggggccagg ccaagcaacc gctgaaagaa gccttcgccc agttcctgcc
     2161 tccagaagct ttgcaggttg cggttccaca acagtgcgaa gagcagcttc tcttcaaaca
     2221 tcggcttggg tggcggcgta aggcggatgc gtttcgccgc cgcgagatcg agaatcactc
     2281 tcgcgttggg cacattggcc aggtcgaaca gctcgaagaa gcgatagacg gcgtcgccgt
     2341 cggcgaaggc gttgaacttc gcatcctgaa ctttgaatcg cgagcttccc aactgattgg
     2401 ccatccagct catctccggc cgataagtgg ccgcttctcc ggacacgacg atgtgcactg
     2461 ttggcgcatc gccgaagatg ctggcgaacc cgcgcaacat cccgtccgct ccgccgagga
     2521 cttccgcgcc catcgccgag taagtctggt tgagccaccc ggtgaggccg atgccgccgg
     2581 ggacggaatc gagctccgtc acgctgagcc catgttctgt taccagcagg tccgggcgga
     2641 tgacgcgcgg cagctcattc ttcagggccg gggaacgctg gagcgcgatc agttcagcgg
     2701 gcttgccctg gtccagccag cgggcgatcc actcgggctg cttgccttcc acgctcttgc
     2761 ggtagagcag gttgaccgcg cgatagaatt gcagcagcac ccgcccgagc gattccacct
     2821 ccttcgccag ggccggtccg agagggtagg ggatcggcga ggtccgccaa tcgtgatctg
     2881 caaacaggcc ctccgccggc atgcgctcac ggacttggcg cgcgcgctcg acggcatcgg
     2941 caggtgcaga aggtatggtc tctgaggtca tgcgttgcta ggtccctctc acgcaggaag
     3001 cctccgggct taggtcctca cctgcccgct gccaatcccg agccacttgt agctggtcag
     3061 ctcctccagg cccatcgggc cacgggcgcc gatcttgtcg gtgctgatgc cgatctccgc
     3121 gcccatgccg aactcgccgc cgtcggtgaa gcgggtcgag gcattccaat acaccgtggc
     3181 ggaatccact tcccccagga actgtttcgc gcgcccctcg tcccgcgtga cgatgctgtc
     3241 cgagtgggcc gaaccgtagt ggttgatatg gtcaattgcc tgcctcaccc catccaccac
     3301 ccgcacgttg aggatgtaat cattatattc ggtgaaccaa tcctgctccg tggccctctt
     3361 aagtgcggaa tacggagtgc ggagtgcgga gttcaggatg gtttcggctt cggcgtccac
     3421 gcgcaactgg accttcttct cgccgagccg ctgtgcgatt gccggcagaa agacgccagc
     3481 caccttgcgg tccaccagca gtgtctccat ggcattgcac acggcgggac gctggacttt
     3541 ggcattcagc gtgattgcct cggccatctt caagtccgca tcggcatcca catagacatg
     3601 gcaaacgccc ttgtaatgct tgatcaccgg caccttcgag cactcggcca cggcgcggat
     3661 gagcccctcg ccgccgcgcg gcatgcacaa atccacatac tgcgtcagcg acagcagttc
     3721 cttgatagcc tcgcggtcgg tcgtcggcac gacctggatg gcgtgttcgg ggaaagccgg
     3781 cagcgtccgc ctgcccgcct cgatcatgat ccgggcgatg gctagattcg agttcaacgc
     3841 ctcctt
//
LOCUS       DATCGS010000165        22618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_559906, whole genome shotgun sequence.
ACCESSION   DATCGS010000165 DATCGS010000000
VERSION     DATCGS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 22618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22618
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559906"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1094
                     /locus_tag="VIL39_03265"
     CDS             174..1094
                     /locus_tag="VIL39_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5480475.1"
                     /translation="MKPIGISKLYKKHLGDWGVNVFAGCEHGCAFCYAPAQPGVRAQP
                     FWVGKTQADWGRFYSERDGLVDALRKQLCTFTPARAVKTAWGQGRILVSFLCDAYQPM
                     EEKLGVTRQVLELLLQAGHRVRIQTRSTLVRRDFDLLLKYKDQVRLGTSLPHLDDNLA
                     KVLEPKAPAPTARLEMLREAAQLGIPVYVAVAPFLPFHALSVLDDLIAQVTPLRPEEV
                     FCEVLNPKGDCVPRVARALAKEYPAESKVVDAYSDAAWSRWTYRVLRHGVDHHASAGF
                     IPWPDTSRAWANYLRADEVGFLNQFLPSGL"
     gene            complement(1560..2357)
                     /locus_tag="VIL39_03270"
     CDS             complement(1560..2357)
                     /locus_tag="VIL39_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480476.1"
                     /translation="MRYEREPPDARTVEGRLRLIRLEYVAPGANRSGGGLKPLSQGRF
                     AERIPLGRERLASYEAGSVPLNFNIGWEICRLANRSQLWLATGESPRIPFLNADVGAA
                     KTKITDRTPFLQGITALWDSLIQASVACGISVTQLPAARPDFSADRRVPPTEPGELVK
                     GRIRVTSTDRSAGQLAVIPEFMPGCTPANAPPSILLTHPGVASVLVFMQQRKRPRGAA
                     LSLLLGSLPLELREDEFLRRLEPTFLLELELAVQKLVGARPPDKDDL"
     gene            2599..2997
                     /locus_tag="VIL39_03275"
     CDS             2599..2997
                     /locus_tag="VIL39_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480477.1"
                     /translation="MLEDKSDANTERHQRLIQWDDLAGQLAIQGRFQESAAAIERLFA
                     GGGPQNKEAARLLDADYPSVEEIRQAAREYVDRECGGEWPEDAWFDVGTSWSVDVWED
                     RGRPRITVYRDFMGADGFRETDTSAGITIQ"
     gene            3061..3204
                     /locus_tag="VIL39_03280"
     CDS             3061..3204
                     /locus_tag="VIL39_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480478.1"
                     /translation="MATFEQLAANDGMIGLHGYEHISPEFEFCQLRSVAVRLENAPET
                     TIT"
     gene            3278..4453
                     /locus_tag="VIL39_03285"
     CDS             3278..4453
                     /locus_tag="VIL39_03285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEY5480479.1"
                     /translation="MSTRFLHTADWQLGKPFAGIEEDDKRVLVQQERINVLNRIGQAA
                     RDQKAEFILAAGDLFDSPTPKRATVSAVCSAVGAMPVPVYAISGNHDHAGPGSLWEQE
                     FFVHEWQQLAPNLHLLLKPEPVDLGQAVIFPCPLLRRHESTDPTAWLRSPKLDLARYG
                     DKPRVVLAHGSVQGFAAPEADDEDAGGGLPNQIDLDRLPEETFDYIALGDWHGAKEVG
                     PKAWYPGTPELDRFPKGGDHAPGKVLVVTARRGGLPDVQPLATARFGWHEVDFQFADD
                     SGLAQLEERVVALIGGRASQDLLRLRLSGVLGIEATTRLERTLDAWKARLLRIKLSNG
                     TILAPSQSEMEALQNRPDDPLISRVSVKLLAVAHGGDEEAAIARIALRELYASCQIH"
     gene            4467..7481
                     /locus_tag="VIL39_03290"
     CDS             4467..7481
                     /locus_tag="VIL39_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEY5480480.1"
                     /translation="MRLISASVQNYRIHRDQTVEFDPHRTVIGGPNECGKSTLAEAVH
                     CALFLRAKVTGEALDRMVSQHGGNPVVAVCFEVAGRRYHLKKGFSGSKGTTTLTEEGG
                     ETWSAELGEEKLAALLRVDAPVGGRSAADRVAKQWAHLWAWQGKAGEDPSEHANMQRD
                     ALLARLKTQGGAAVMQSDVDARVASRFATLREQLFVRNDTPRAGSDWAGALQACNDAI
                     QTESDSRMAFERLQQAAGDFELATIEIKACEERLELLAAEKGEVEIKLQQVGNLQVDE
                     ASQLAEANAAGERYNALESNDQRISECRSGIQARENALAPKRSAVRQLEEVAESKKQL
                     AGQAVRQFEDAGEGLRKLRFRHELARAYVRLFELTAESDRLATKAARVRQSKDDLTEV
                     ERNLAAIPAVDNAKLKKLQSLDRKRSDAEVALKAMAAGLEVLEAAEPVSAGDTSFAVG
                     QTQILTEDTEIRIGSGTRLRVRPGGGTTLAEVRTAVQDAQRELQKALDKLGAASLAEA
                     SDFCARRQQLDQRAGEINATLEGLGADNVGAEVAGCGVAKANAEAEVARLGVLATEFT
                     PPANLEGAVSWEGSAGEQLRQAEAEERSLKTTRTAALDAQTGAEAAFNEQNANLEAEK
                     RQIEEVKAQLNVLIQAYGEDAPRAKTLQELLNAKNAAEGRLGATREALRELQPELLAT
                     RKRNCERGFETNRSQLGEWGQKLAVARNILASDGITDPKGALAEAEAKARSAKEHLRT
                     VERRAKAIQLLHRLFLEEQRALADLFTLPLAEKISGYLRCLFGPGAQAKVTLQENTFT
                     GLEVVRAGHGLGSFQFDTLSGGAKEQVAAAVRLAMAEVLAEEHDKCLPLVFDDAFAYS
                     DPQRVKTLQDMLGLAAERGLQIIILTCNPSDYTNLGAKQVSLLAARPMAQAPQSQPVV
                     ALTGEGEGAHGMELMEAPVNAGVTAEQRQAFLVALKAVGGHSGNTNLRTALGWEATHY
                     DTVKATLVAEGLLMTGRGKGGSVSLVPVGQDP"
     gene            7486..10779
                     /gene="recC"
                     /locus_tag="VIL39_03295"
     CDS             7486..10779
                     /gene="recC"
                     /locus_tag="VIL39_03295"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEY5480481.1"
                     /translation="MSLILYKSNRLEILARHLVEHTLQSPLSSPFLSEQIIVQTQGMA
                     QWLKLELCQRQGILANVEFPFPRAFLSRLTVDLLPEPVRAGAIEPEALTWRVMGKLDA
                     LLPQPVFSDIKNYLATSEDPRRKFQLAERVATLFDQYSIYRPEWIAAWQKGEETHWQA
                     ALWRAVMTEGLACQGKFLYELIQALNGPACETGKLPQRLSIFAPNNLPPIYLAVFEAL
                     ARRLAVHLFCLCPCREYWGDTPSPREAERIQAMAGDGNIDPGDLHLEGANPLLTSWGR
                     TGRGFQRLVTDLEGIEQEIEDYAEPETKHLLGSVQSSILKLEDEESMPLEKVEIKHDD
                     RSIQVHSCHSPLRELQVLRDHLLEWFAADPTLGAQDILVMLPDLEAYAPFIKGVFDNA
                     EEGAPAIPYTLAERGARQESALVDGFAKLLQLASSRLGASAVVDFFETAAVRRRFGVA
                     EDELSQIRQWIQNSGICWGRDANHRVKLGLPRFAEHTWEHGCSRLLLGYAMADEGDAL
                     VHGLLPCEGIEGTATRLLGRWLDFLRQLFAALDELSVNRTLSEWASSLNELLDRLFLP
                     DSDEELAANAIRQILDGLRQQQAASGFQEAVPLTVVLERIVPKFAENQPGKAFLRGSV
                     TFCGLNPMRTVPFRVVCVLGLQDGSFPRCPAPPSIDLMAKQPRTGDESRRDDDRYLFL
                     ETLLAARDRFYLSYVGQSVRDNSPRPPSVAVSELLDFIASRFRLEGEKEAKQESRIIS
                     QLLVTVHRLHAFSPAYFRQGGGRDPRRFGYSPASAKISTFLANPASRASVEPFLVQAL
                     GDLSEEWQTVTLGDLQSFYRNPSRFFVEKRLEFRLPTDKDLLQEEEPFSIDGLEAFGF
                     KQEILDSIFSRKPRSQLLAVWKGSGQLPPGPAGSLLANDLFSDAGPVAKRLQQRVGQA
                     QRMPQRFDLQVGDFKLEGQLALFPGVGLVHYRAAKVDRKKKGEAHLKLWIEHLLLQLT
                     GWDGPKCSYLIGEDEIWQFNEVANAEELLRGLLGLYRKGLAAPLPFFPQSSFVFAIGP
                     GPRTTKSAAELAMETWEGNEEDEFGEGDLEDPYFNLCFRNHPDPLGEEFQELASQVVG
                     PLLNHQIKL"
     gene            10783..14442
                     /gene="recB"
                     /locus_tag="VIL39_03300"
     CDS             10783..14442
                     /gene="recB"
                     /locus_tag="VIL39_03300"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEY5480482.1"
                     /translation="MTFDLVNTPLSKGVTRLEASAGTGKTFALAGIFLRLLLEEKVPA
                     SDILVVTFTEAATAELRDRIRRRLSEALAVLEGKPSQDPLLVALKERVQARHDAAVRS
                     LRNALEIFDLVSIYTIHGFCQRTLQDSAFESGILFDVELVADQDDLIRETAADYCRNQ
                     LHQCDEVLVGAAIHTKLNPEALSRMLKQFLTYPELQLLPAAPPRPIEIITRELREAFA
                     AFASAWQAMANDRPALVNYFNPAKKWVTGEYAKHDLLEEYMDQLDSCLDVATLSSQLW
                     QGIEFFSKSAIEAKKGKGKEMPPPPTPLFDQCQALTDLAREHAVAHRVVFLQSAGRML
                     GERKQQAKQQSYDDLITRLAAALDSASGPALAQTVRRKYRVALIDESQDTDPLQWKIF
                     QRVFAASQDHWLYLIGDPKQAIYGFRGADVNTYLQAAATANQEYSLDTNWRSESALVN
                     SVNALFSSAGKTAAFLEENILFEKVKPGPKADESPLLFPADDHRPPPFQVWCWESESG
                     TVTSKQAKRQLPTAVAAEVSRLLNAKVRLGENATLRPRDIAVLVESHKQARWTQAALH
                     ELHIPSVEQAMESVLESGEAREVQWILAAILAPGREAAVKSALTTDALGVSCDKLQAL
                     VANESSWQARLQSFARYRDLWEEDGFFFMFSQLVREEEVIENLLRFPDAERRITNLLH
                     VAELLETACKAEHLGPSRLVQWLEERRMSEEAPPDAYQLRLESDEDAVQIVTIHRAKG
                     LQYPVVFCPFVTKDAKPRSIKVNRQKVLDVVLYHDPGSKRLTWDLNAEPEETHAQLAA
                     KEQLAEKVRLLYVALTRACNRCYLVSARYARNKSTALAWLLRSQGGEMGEQVDPVTVL
                     DKESVVPSGWKSRWNDIASASADPAGGKPGIAVDDLPLEPGEPWQPEQPASGGLTPKI
                     CVREIRPSWFLSSFTQLSSHLSLRHALASETDHPDHDEAAAGTLSVGTEDEATEPLTG
                     IFALPASARTGDCLHKIIERFDFADADEATAKSLVRQQLEAYDLYDEERARAVIGMLE
                     RLRHTPLDPANPNLILSAVSSAERLTEMEFHFPTGHLDGARLLDLIRSPNPSSAPASS
                     RAAVPVSRIQAFLKGFIDLVFRFEGRYHVVDWKSNLLGTKVEAYTREAMTRAVEESCY
                     DLQYHIYVVALDKYLRKRVPGYEFETHFGGVHYVFLRGLAPGRPDLGVYHDRPSAQKI
                     ECLSSVLGTFEEVKS"
     gene            14439..16373
                     /gene="recD"
                     /locus_tag="VIL39_03305"
     CDS             14439..16373
                     /gene="recD"
                     /locus_tag="VIL39_03305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEY5480483.1"
                     /translation="MTQAISPLLSVLESDAFNLTDRHLANLLVKLSGKECPSLGLAAA
                     LVSRELADGHSCISLDAFGGRSFLDSRPGNGDSICCPPRAEWERLLLETSVVGRPGED
                     KPLVLDEAGRLYLHRYWRYEQAVAQDLLARIGMESLALDLKTLAAGMNRLFPGIPGQI
                     NWQQVGAFAAVRQRFSVITGGPGTGKTWTVARVLALLLEQPAGQNLRVKLAAPTGKAA
                     ARLQESLGQSLESLACSEVIKARLQAKDLTTTIHRLLGPIPNSARFQHGPDNPLPVDV
                     LVVDEASMVSLSLMARLLAALKKEDVRLVLVGDKDQLPPVDPGGVLGDICRAASVNSF
                     SPSFCQAFQLCNGVALPGKGAVLNGALADTVVQLDVNYRSGEALALHTLSTAVNAGAT
                     SKVLELLRDADAGGAVVAWQSLPQRPRLKEALRETVRSHYRLVLQAASPQEALQALGR
                     FRILCALREGPYGTLAINRLVEEILAEELPALAEKMRFGSYPGKPVMVTSNNYILKLF
                     NGDTGVFWTGNGGSSLVHFRDESGSVRAIARERLPEHEVVYAMTVHKSQGSEFERVLL
                     MLPDRENPVLTRELFYTGLTRARKSVRILADEGILRSAIETRAQRFSGLSDALCSMSH
                     RAVQLGLSPVPESGTPIVHI"
     gene            16370..17026
                     /locus_tag="VIL39_03310"
     CDS             16370..17026
                     /locus_tag="VIL39_03310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5480484.1"
                     /translation="MSHIALLGDSIFDNGAYVRPGPDVITQLRRKLPAGWQATLFARD
                     GDVTSGVAGQLEVLPASATHLVISVGGNDALQHQGVLAERICFVAEALQKLRAIQVEF
                     SRYYRSMLTAVLHCGKPTVVCTIYDGCHPDIREQQVRVAALALFNDCIIRAAIERELP
                     VLDLRAICTQAADYANPIEPGVAGGEKMAAAVIGVVQRHDFTGKSSAIYQGEARPANL
                     "
     gene            complement(17045..17533)
                     /locus_tag="VIL39_03315"
     CDS             complement(17045..17533)
                     /locus_tag="VIL39_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEY5480485.1"
                     /translation="MAGEYPGDKADAKARAKIQRTLDCGITFFLDLTEPGEYSLKRYD
                     QILGAATVAGKQTNYQRIPIKDTSVPARSEMTQILDTIQNALAQGHVVYVHCWGGVGR
                     TGTVVGCWLVRSGLSGQQALGQVARHWGAMSEEKRKRRPESPEFPSQHDFVRAWSEKP
                     GS"
     gene            17688..18095
                     /locus_tag="VIL39_03320"
     CDS             17688..18095
                     /locus_tag="VIL39_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5480486.1"
                     /translation="MAKLKKLKRAKGGNSNNRGNRRIIVHNNACISLRCRKDSSRSSP
                     LSETAFVDCKIFGPWAESLAKHKKGDMALVSGRLKTDSWEKDGRKQSKLIVICDSVRF
                     FVPQNGSSASASVAENESAMQEELNDKNGKPPF"
     gene            18241..19758
                     /locus_tag="VIL39_03325"
     CDS             18241..19758
                     /locus_tag="VIL39_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480487.1"
                     /translation="MREPAWECNQTVFADFHRRHGYRRNVLNCAIPFARAKTLTDFTM
                     PQGWNLDKSLIASVGFGAKLPAGLENWSYTPKIQAVKQLPQMKDLPREHSTLMHSPWF
                     QSPSQAMGLGLNARHLRYHYELWNDIQPKTLRGVCTTLVYVTPQIGFALTPHIKLDRV
                     FRYFGALSRMAGPTQLFPASYLYPDARLHGLEKVPAAMFADILPHLGSSFPLMDESTN
                     PWDVVVRLNGPDCYYGYPLSRVSNPVEDVTLSNATAGSMTLPNGQQVKARSSETFCYA
                     TLDPKLKDALKMDRDAFHAAYCLDRGVITHDGLPFMLPVEMQSAVFAVILTAWELEMD
                     KPGDLTSYTLKTQHSAIRGYNFYQKIEPYYSCFMRLVHDLSQDQNFETYPLSSHLTHS
                     WLGGNLLLSNQRSEAEEVQGINFLRNYYLQQLDWSMGVYNLWNTVEFRRGSDLESQLD
                     HAGVAFHQMRPQLRHDVHLFQRPLRRASFWPDFNPKCLEFDQTYMAEGLPTPHER"
     gene            19828..20385
                     /locus_tag="VIL39_03330"
     CDS             19828..20385
                     /locus_tag="VIL39_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480488.1"
                     /translation="MNTESNTSPQNLIVCQFTRDCIEKQQLAPFLDYWAPHKLPHGPA
                     LRTLMNKMFFHIEGYDGPRAVCTIPEVRSFCQAFFESWPFWFFVAHLDSPSLLIMVFA
                     CLQKLRVVDEEAGGVCHVQIDAVELNEFIERGLVGMECLCKRAGMSPAEISLRSMQII
                     EYFQTDWSSSSAQAADGKPETGRWN"
     gene            20734..21240
                     /locus_tag="VIL39_03335"
     CDS             20734..21240
                     /locus_tag="VIL39_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480489.1"
                     /translation="MTERLEIYRATSNRYRQWYADQEEHGGRCFYWSLTLMGVLLENG
                     YRALIQAGSMSWPIVPPGQDDGRSPTHFSYEWSPWREESQAALKLGLLPEIHVWVGLP
                     DQNELLDFATKFLPEQAAKEGLAWRTPNPPDFLWCGPSELPEGVLYKPDLNAIGFILD
                     RISKTNHR"
     gene            21264..>22618
                     /locus_tag="VIL39_03340"
     CDS             21264..>22618
                     /locus_tag="VIL39_03340"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5480490.1"
                     /translation="MPNWCLNKLTITGPEADVQAFKTKAVGHSPWEEPEGEPDALNFH
                     SLVPIPENLLAAGYDGARYDWEMANWGCKWGASGPGILDEREGHVEYEFYAVLRASQV
                     PLAAGVSAPSGENGPPPHQVIKLGIDVHLDRYVVVRQIDGGAPQPPQRFSPTQFLEWA
                     KKQTALAQQVYSCYEAGPFGYRLHRKLKELGITNYVIRPRDWDEYGKKVKTDKRDAKE
                     MVLNLDRYVSGNQEAFCVVRVPTEAEEQARSRSRQRESWQKEKQRLAAQGRSHALYYG
                     AHLEGAWWTGGLWKEMVVPPIVLELLEPLRRLIQALEQELKTLTQALTTATPKQLPMG
                     LGKLTYEVLEREVGDWHRFHNRRQVASYTGMCPREDSSAQRRFQGSINKHGNPRMRTV
                     LVEASWRLVQYQPTYKPVARWLPVLTHPKTTRSKRKQIIVAIGRQFGVDWWRVRTRRC
                     QA"
BASE COUNT         4639 a         6731 c         6939 g         4309 t
ORIGIN      
        1 gagcaggccc gccaattggc cgccatcgag cgcgacctgg cgctggcctt aatccagcgt
       61 tcgttgacgc gcaattaaag ggtctcgcag ggcaagccga tggaaagagc gaaccacgat
      121 tggagccatc aacaccgtat caattaacca ttaacaaagc agaaagataa gacatgaagc
      181 cgattggcat ctcgaaacta tacaagaagc acttgggcga ctggggtgtg aatgttttcg
      241 ccggttgtga acacggctgc gccttttgct acgcccccgc gcagcccggt gtgcgggcgc
      301 agcctttctg ggtcggcaaa acccaggcgg actgggggcg gttctatagc gagcgcgatg
      361 gtttagtgga cgcgttgcgc aaacagcttt gcacctttac accagcccga gcggtgaaga
      421 ccgcctgggg ccagggtcgc atcctggtat cgtttctgtg cgatgcgtat caaccgatgg
      481 aagagaagct gggggtcacc cggcaggttc tcgagctcct cctccaagcg ggccatcgcg
      541 tccgcattca aacgcggtcc acgttggtgc gccgtgattt cgatctgctc ctcaaataca
      601 aagaccaggt gcggttgggg acatccctgc cccacctcga cgataatctg gccaaggtgt
      661 tggagcccaa agcccccgca ccgactgccc gcctggagat gctgcgcgaa gcggcgcaat
      721 tgggtattcc cgtctacgtg gcggtcgcgc cattcctgcc ctttcacgca ttgagcgtcc
      781 tcgatgacct catcgcgcag gtcactccgt tgcggcccga agaggttttt tgcgaagtcc
      841 tcaacccaaa gggcgactgc gtgccgaggg ttgcgcgggc gctggccaaa gagtacccag
      901 ccgagtccaa ggtagtagac gcttactctg acgcggcatg gtcgcggtgg acataccggg
      961 tgctgcggca cggcgtggat caccatgcca gcgctggatt tattccgtgg cccgacacca
     1021 gccgcgcctg ggccaattac ctgcgggcag acgaagtggg cttcctgaac cagtttctgc
     1081 cctccggcct gtaaacacta ccgggtcatg cggagatccc caatcaccat ggcgactgat
     1141 ctgaacgctt cggaacgcaa agcctttcca atggctgatc gctgggaagc cgtcgcggag
     1201 ccggcgcggg agtgcaacca ggcggtcttt cgggatttct tccagcggca cggccaccgc
     1261 cgcaacgtcc tcaactgcgc cataccgtcc gcacgcgcca aggcgctgac cgacttcacc
     1321 atgccggaag gctggaacct cgacaagagc ctcatcgcca gcgtgggctt tggcggcccc
     1381 gttgcccccg ggtttggaga actggagctg caccccgaag atagcggcta tcggcgaatc
     1441 ccaatgaggg tccagaacga atacgctagc gatcgtggtc gccgatgtgg tggccaggtg
     1501 cctgtccgga tcggtcaggt tgagacaccc ctgcgggcca acgccacccg caagcggaat
     1561 cataggtcat ccttgtccgg cggtctggcg ccaaccagtt tctgcacggc tagttcgagc
     1621 tcgagcagga aggtcggctc cagccggcgc aggaattcat cctcccgcaa ttccagcggg
     1681 agcgagccca acagtagtga cagcgcagcg cctctgggcc gctttctttg ctgcatgaac
     1741 acaagcacgg aggcgacgcc cggatgagtc aacagaatcg atggcggcgc gtttgcaggt
     1801 gtgcagcccg gcatgaattc cgggatcaca gccaactgtc ctgccgaacg atccgtggaa
     1861 gtgacccgga tcctgccctt caccaactct ccgggctctg tcggtggcac ccggcggtcg
     1921 gcagagaaat ccggtcttgc agccggcagt tgggtgacgc tgatgccgca cgccaccgag
     1981 gcctggatca agctgtccca gagggctgtg atgccctgca ggaagggagt ccggtcggtg
     2041 atttttgttt ttgcggctcc aacgtcggcg ttgaggaatg ggatccgggg gctttcacca
     2101 gtcgcgagcc agagctgcga acgattggcg agtcggcata tctcccaccc gatattgaaa
     2161 ttgagtggga ccgaacctgc ctcgtaactg gccagtcttt cccgccccag cgggatgcgc
     2221 tcggcaaaac gtccctgcga caatggtttc aaccctcccc cggatcgatt ggcgccgggc
     2281 gcgacgtatt ccaaccggat cagccgaagg cgaccctcga ccgtcctggc atctggtggc
     2341 tcccgttcat atcgcataat tcagttttat cagtgaatgc tgccccggat aatgatagcg
     2401 caagaaatca cttccacgcc aaggccattg atagatttca atatagcgag gtcgccgacg
     2461 caaaaaagtt aaaaaagggg ttgaaagcat tcaaaaacaa tatacaataa gacactgggg
     2521 agtgaacctc ggtgtgggtg tgaaggcaac ggttgctgtt ttgattatat aatggtagcg
     2581 cataacaata ctattcatat gctggaagac aaatcagatg caaatacaga gcgccatcag
     2641 cgcctaatcc aatgggacga cctggccggg cagttggcca ttcagggcag gtttcaggaa
     2701 tccgccgccg cgattgagcg gctctttgcc ggtggcggtc cacaaaacaa ggaggctgcc
     2761 cggctcctcg atgcggacta cccgagcgtc gaggagattc ggcaagccgc aagagaatat
     2821 gtggaccgtg agtgcggggg tgagtggccc gaggacgcgt ggttcgatgt tgggacctca
     2881 tggtctgtcg atgtgtggga ggaccgcggt cgtccgcgga tcaccgtgta tcgcgatttc
     2941 atgggtgccg atggttttcg cgaaaccgat acctccgccg gaatcacgat tcagtaattc
     3001 tcaaggttga gcctcgtcct atccagaggg atacatccca ataggtgaaa ccaacagctt
     3061 atggcaacat tcgaacagct cgcagctaat gacggcatga tcgggttgca tggctatgaa
     3121 catatttccc ccgagtttga gttttgccag ctgcgatctg ttgcggtgcg gttggaaaac
     3181 gcacctgaaa ctactataac ctgattccag aacgcggaag gcttgcgcag tgcgctacaa
     3241 cctgcacaat cgcctgcgcg ggttaccatt gagaaacatg agcacgagat tcctgcacac
     3301 cgccgattgg caacttggaa agcccttcgc aggcatcgag gaggatgaca aacgcgtcct
     3361 ggttcagcag gagcgcatca atgtcctgaa tcggattggg caggcagcga gggatcaaaa
     3421 ggccgaattt attctcgcag cgggggatct ttttgattcg cccacaccca agcgggccac
     3481 cgtatccgct gtgtgcagcg ccgtcggcgc catgccggtg ccggtctatg ccatctcggg
     3541 gaatcatgat cacgccggac ctggcagtct ttgggagcag gagtttttcg tgcacgaatg
     3601 gcagcaactc gcccctaatc tccacctgtt gctcaaaccg gagccagtgg acctggggca
     3661 ggctgtgatt ttcccctgtc cgctgctccg gcggcatgag tccacggacc cgacagcctg
     3721 gttgcgctcc ccgaaactgg atctcgcccg ctatggcgac aagccgcggg tagttctcgc
     3781 ccacggcagc gtgcaaggct tcgcggcgcc ggaggccgat gacgaagatg ctgggggagg
     3841 attgcccaat caaatcgacc tggaccgtct gcccgaggaa accttcgact atatcgcttt
     3901 gggggactgg cacggagcca aggaggtcgg gcccaaagca tggtatcccg gcacgcctga
     3961 gctggaccgg tttcccaagg gaggcgacca cgctcccggc aaggtgctgg tagtgactgc
     4021 gcggcggggc gggctccccg acgtgcagcc gctcgcgacc gcgcgtttcg ggtggcatga
     4081 ggttgatttc cagtttgccg atgattctgg cttggcccaa ctggaggaga gagtcgtggc
     4141 gctgattggc ggtcgcgcca gccaggatct gctgcgactg cgcttaagcg gcgttctcgg
     4201 gattgaggct accaccaggt tggaaaggac tctcgatgca tggaaagcgc ggttattgag
     4261 aataaagctc tcgaatggaa ccatattagc gccttcccaa agtgaaatgg aagcgttgca
     4321 gaatcggcct gacgatccgt taatttcgcg ggtttccgtc aagctccttg ccgtggcgca
     4381 cggcggggac gaagaggcgg ctatcgcgcg gatagctttg cgcgagctct acgcgagctg
     4441 ccaaatccat tgaacagagg attattatgc gactgatttc agcttctgtt cagaattacc
     4501 ggattcaccg cgaccaaacg gtggaattcg acccgcatcg cacagtgatc ggcggtccga
     4561 acgaatgcgg caagagcacg ctggccgaag cggtccattg cgcgctcttt cttagagcga
     4621 aagtcaccgg cgaagccttg gatcggatgg tgtcacagca tggcggtaac ccggtcgtgg
     4681 cagtgtgctt tgaggttgca gggcgccgct accacctgaa gaaggggttt tccggctcaa
     4741 aaggcacgac cactttgact gaggagggcg gcgaaacatg gtccgccgag ctcggcgagg
     4801 aaaaactggc ggccttgctc cgcgtggacg cgccagtcgg gggcagaagc gccgcggatc
     4861 gggtagcaaa gcaatgggca cacctgtggg catggcaggg caaggccggc gaagatccct
     4921 cggaacatgc aaatatgcaa agagacgctc tccttgcccg gctaaaaacc cagggtggag
     4981 cagcggtgat gcagtccgac gtggacgcgc gggtggcaag ccggtttgcg actttgcggg
     5041 agcaactgtt tgtcagaaat gacacccccc gggctggttc cgattgggcg ggcgccttac
     5101 aggcatgcaa cgacgctata cagacggaga gcgactcccg catggctttt gaacgtctgc
     5161 agcaggcggc cggcgatttc gaactggcga ctatcgagat caaggcatgt gaagaaaggc
     5221 tggagctgct cgctgccgaa aaaggcgagg tcgaaatcaa gctccagcag gtgggcaacc
     5281 ttcaggtgga cgaggcatcg caactggccg aagccaacgc ggcaggcgaa cgctacaatg
     5341 ctttggaatc taatgaccaa cgaatcagcg aatgccgcag cggaatccag gctcgggaga
     5401 acgcgctggc tccgaagcgc tctgccgtga ggcaattaga ggaagtcgct gaaagcaaaa
     5461 agcaactggc tgggcaggcg gttcgccagt ttgaagacgc gggcgaggga ctgcgtaaac
     5521 tccggttccg gcacgagttg gcgcgcgctt acgtcaggct tttcgaactg actgcggaga
     5581 gcgaccggct cgcgacgaag gcggccaggg tgcgccagtc caaggatgat cttactgagg
     5641 tggaaagaaa cctcgccgca ataccggccg tcgataacgc gaagctcaag aagttgcaga
     5701 gcctggaccg caagcgctcc gatgcggaag tcgcgttgaa ggcgatggcg gcagggctgg
     5761 aggtgctgga agccgctgag cccgtcagcg cgggagacac gtcgtttgcc gtggggcaga
     5821 cccagattct caccgaggac acggaaatcc ggatcggctc aggaacacgg ttgcgtgtga
     5881 ggcctggcgg aggaacgacc ctggcggaag tccggacggc tgtgcaggac gcccaacgcg
     5941 agctgcaaaa agctctcgat aaactgggcg cggcatcgct tgccgaggct tctgatttct
     6001 gcgcccggcg gcagcaactg gatcagcgcg caggcgagat taacgcgacc ttggaaggac
     6061 tcggcgcgga caatgtcgga gcggaagttg cgggttgcgg agttgcgaag gcgaatgccg
     6121 aagccgaggt ggcgaggctg ggcgttctcg cgacagaatt cacgccgcca gccaacctgg
     6181 aaggggcggt gagctgggaa gggtcggcgg gtgaacagct tcggcaggcg gaagccgagg
     6241 agcgctccct gaaaacgacg cgcaccgcag ccttggatgc gcagaccggg gcggaagccg
     6301 ctttcaacga gcagaatgcc aatctggaag ccgaaaagcg ccagattgaa gaggtaaagg
     6361 cacagttaaa tgtgctgatc caagcctatg gggaggatgc tccacgggca aagacattgc
     6421 aggaattgtt aaacgccaag aacgctgctg aaggcaggct tggcgccacc cgagaagcgc
     6481 tgcgagaact ccagccggaa ctgctggcga cccgaaagag gaactgcgag cggggtttcg
     6541 agacaaatcg aagccaactc ggtgagtggg gacaaaagct ggctgtggct cggaacattc
     6601 tcgcctccga tgggataact gatcccaagg gagccctggc cgaggcggag gccaaggcgc
     6661 gttcggccaa ggagcatctg cgaacggttg aacgcagagc caaggccata caactgctgc
     6721 accgcctgtt cttggaggaa cagcgtgcgc tagccgatct gttcaccctt ccattggcgg
     6781 aaaagatctc cggctacctg cgatgcctct tcgggccagg cgcgcaggcc aaggtcacgc
     6841 tccaggagaa cacctttacc ggcttggaag ttgtgcgcgc gggtcacgga cttggcagct
     6901 tccaatttga caccctcagc ggcggagcca aagagcaagt ggcggccgcc gtccggcttg
     6961 ccatggccga ggtgctcgcc gaggagcatg acaaatgcct gccgctggtc ttcgacgacg
     7021 cctttgccta ttcggacccg cagcgggtga agacgctcca ggatatgctg gggcttgcgg
     7081 ctgagcgcgg ccttcaaatc ataattctga cgtgtaaccc ctccgactac accaatcttg
     7141 gcgctaaaca agtgtcgctg ctcgcggcac gacctatggc tcaggcgccg caatctcagc
     7201 cagttgttgc cctgactggt gaaggagagg gggcgcatgg aatggaactg atggaggccc
     7261 ccgtcaacgc cggggttact gcggagcaac gccaggcctt cctcgttgcg ctaaaggctg
     7321 tgggcgggca ttccgggaac acgaacttga ggacggctct gggttgggaa gcgacccatt
     7381 acgataccgt caaagcaacg ctggtcgccg aaggcctgtt gatgacgggc cggggcaagg
     7441 gtggatccgt ctcgcttgtg ccggtgggac aagacccctg aactcatgag cctcattttg
     7501 tataagagca accggctgga aatcctggcg cgccacctgg tagagcacac gctccagtcg
     7561 ccgttaagtt cgcccttctt atccgagcag atcatcgtcc agacccaagg catggcccag
     7621 tggctcaagc tggagctttg tcagcgccag ggcatcctcg ccaatgtcga gttcccgttt
     7681 ccccgcgcat ttctttccag gctgacagtg gacctgctgc cggagcctgt gcgggcgggc
     7741 gcgattgagc cggaagcgtt gacctggcgc gtcatgggca agctcgacgc cctcctgccg
     7801 cagccggttt tcagcgacat aaagaactat ttggccactt ccgaggatcc gcggcggaaa
     7861 ttccaattgg ccgagcgcgt cgcgacgtta ttcgaccagt acagcatcta tcggcctgag
     7921 tggattgcgg cgtggcagaa aggcgaggag acccactggc aggccgccct gtggcgcgcg
     7981 gtcatgaccg aggggctggc ttgccagggc aagtttctct acgaactgat ccaggccctc
     8041 aacggcccag cctgcgagac gggcaaactg ccacagaggc tctccatttt tgcccccaac
     8101 aacctgccgc cgatttacct ggctgttttc gaggcgctgg ctcgccgtct cgcggtccac
     8161 ctattttgcc tgtgcccctg tcgcgagtat tggggcgaca ccccctcccc ccgcgaggca
     8221 gagcgaatcc aggcaatggc cggggatggc aatatcgatc ccggtgacct gcacctggaa
     8281 ggcgccaacc cgcttctgac ttcctggggg cgaacgggac gcggcttcca gcgcctggtc
     8341 acggatctcg aaggcatcga acaggagatt gaagactacg ctgaaccgga gacaaagcac
     8401 ctccttgggt ccgtccaatc gagcattctc aaactggagg acgaggagtc catgcccctg
     8461 gagaaggtgg agataaagca cgatgatcgc tcgatccagg tccactcctg ccacagccca
     8521 ttgcgcgaac tccaggtctt gcgggatcac cttctcgaat ggtttgccgc cgatcccact
     8581 ctaggtgccc aggacatcct cgtcatgttg ccggaccttg aggcctatgc cccgtttatc
     8641 aagggcgtgt tcgataatgc cgaggaaggg gcgcccgcaa tcccctatac gctcgccgaa
     8701 cgcggcgcgc ggcaggaaag cgcgctagtc gacgggtttg ccaaactgct gcagctggcc
     8761 tccagccgcc tgggtgcctc agcggtggtg gacttcttcg agaccgcagc agtgcggcgg
     8821 cgtttcgggg tggccgagga cgagctttct caaatccgcc aatggattca gaactcaggt
     8881 atctgctggg gtcgggatgc gaaccaccgc gtaaagctcg gcctgcccag gtttgcggag
     8941 catacctggg aacatggctg tagccgactt ctgttgggct atgcgatggc cgatgaagga
     9001 gatgccctgg tccacggcct tctgccgtgc gagggcatcg aaggcacggc taccaggctt
     9061 ctggggcgtt ggctcgattt tctgcggcag cttttcgcgg cgctggacga actatccgtg
     9121 aatcgcactc tgagcgaatg ggcgagctcc ctgaacgaac tcctggaccg cctctttctg
     9181 cctgactcgg acgaggagtt ggctgccaac gcgatccggc aaatcctgga cggcttgcgc
     9241 cagcagcagg cggcgtcagg gttccaggaa gctgtccctt tgacggtggt ccttgagcgc
     9301 atcgtcccca aattcgccga gaaccagccc ggcaaggcct tcctgcgagg cagcgtgact
     9361 ttttgcggcc tcaacccgat gcgcacggtg cctttccggg tggtgtgcgt cctcggattg
     9421 caggacggga gtttcccgcg atgcccggcg ccgcccagca ttgacctgat ggcaaagcag
     9481 cccaggactg gggatgagtc gcggcgcgac gacgatcgtt atctgttcct ggagacgctg
     9541 cttgccgcgc gggaccgctt ctacctgagt tatgtcggcc agtccgtgcg cgacaatagc
     9601 ccgcgcccgc cgtcggtggc cgtgagcgag ctgctggact tcattgcctc ccggttccgc
     9661 ctggaagggg aaaaagaggc caagcaagag tctcgcatta tcagccaact actggtgacg
     9721 gttcataggc tgcacgcgtt cagtccggcc tatttccggc agggcggcgg tcgggatccg
     9781 cggcggtttg gctattcccc tgcctctgcc aagatcagca cgtttttggc aaatccggcc
     9841 agtcgtgcat cggtcgagcc ttttctggtg caggcgctgg gcgaccttag tgaggaatgg
     9901 cagaccgtca cgctaggaga cctccagtct ttttaccgca acccctcccg cttcttcgtc
     9961 gagaaacgcc tggagttcag gctgcccacg gacaaggacc tcctgcagga ggaggagccc
    10021 ttctccattg acgggctgga agcgttcggg ttcaagcagg aaatcctgga ttcgattttc
    10081 agccgaaagc cgaggtcgca gcttctggct gtgtggaagg gctctggtca gcttccgcca
    10141 gggccggccg gatcgctgct ggccaacgat ttgttttccg atgctgggcc cgtggccaag
    10201 cgtcttcagc aacgggttgg ccaggcccaa agaatgcccc agcgattcga cctgcaggtc
    10261 ggcgacttca aactggaagg ccaactggcc ttattccccg gggtcgggct ggtgcattac
    10321 cgggcggcca aggtggaccg aaaaaagaag ggggaagctc atctgaaact ttggattgag
    10381 cacctgttgc tgcaactcac tgggtgggac gggcccaaat gcagttacct catcggcgaa
    10441 gatgagatct ggcagtttaa cgaggtggcc aatgcggaag aactcctgcg cgggttgctg
    10501 gggctttatc gaaaaggcct ggcggccccg ctgccgtttt tcccgcaatc ctcgttcgtg
    10561 tttgcgatcg ggcctggccc caggaccacc aagagcgccg ccgaactggc gatggagacc
    10621 tgggaaggca acgaggagga cgaatttggg gagggcgatc tggaagaccc gtatttcaac
    10681 ctctgtttca ggaaccaccc cgacccgctg ggtgaggagt tccaggagct ggcctcgcag
    10741 gtggtcggcc cgctcctaaa ccatcaaatc aagctgtgaa ccatgacctt tgacttggtt
    10801 aatacgcccc tcagcaaggg agtcactcgg ctggaagcca gcgcgggcac cggcaagacc
    10861 tttgccttgg ccggcatttt tctgcgtctg ctccttgagg agaaagtgcc tgccagcgac
    10921 atcctggtcg tgaccttcac cgaggcagca acggcggagc tgcgcgaccg gatccgccga
    10981 aggctcagcg aagcgctagc ggtcctggaa ggcaaaccga gccaggaccc gcttctggtc
    11041 gctctgaagg agcgggtaca ggccaggcac gatgcggctg tcagatccct gcgcaatgcg
    11101 ctggagattt tcgacttggt ctcgatttat acgattcacg gcttttgcca gcgaacgctg
    11161 caggacagcg cctttgagag cggcatcctc tttgacgtgg agcttgtcgc cgaccaggat
    11221 gatttgatcc gcgagacggc cgcagattac tgccgcaacc agcttcatca atgcgacgag
    11281 gtgctggtcg gcgccgccat ccataccaag ctcaatcccg aggccctgtc ccggatgctc
    11341 aagcagtttc tcacctatcc ggagctgcag ttgctgccgg cagcgccgcc ccggccgatc
    11401 gagatcatca cccgcgagtt gcgggaggcc ttcgctgcgt tcgccagcgc ctggcaagca
    11461 atggcaaacg accgcccggc gctcgtgaac tatttcaacc cagccaagaa atgggtcacc
    11521 ggcgaatacg ccaagcacga cctgctcgag gaatacatgg accaactcga ctcctgcctc
    11581 gacgtcgcga cgctttcatc ccaactgtgg caaggcatcg agttcttttc caaatcggcg
    11641 attgaggcga agaagggcaa aggaaaggaa atgcccccgc caccgactcc gttattcgac
    11701 caatgccagg ccttgaccga ccttgcccgg gagcacgcag tcgcccaccg cgtggttttc
    11761 ctgcaaagcg ccggcagaat gctgggagag cgaaagcaac aagccaagca gcagtcctat
    11821 gacgacctga tcacccgact ggcggccgct ctggacagcg cctccggccc cgccctggcc
    11881 caaactgtgc gccgcaagta tcgcgttgcc ttgatcgacg aatcccagga caccgacccc
    11941 ttgcaatgga agattttcca acgggtgttc gccgccagcc aggaccattg gctctacctg
    12001 ataggcgatc ccaaacaggc gatttacggg tttcgcggcg ccgatgtaaa tacctatctc
    12061 caggccgcgg ccaccgcgaa ccaggaatat tccctcgata ccaactggcg ctctgaatcc
    12121 gccttggtca actccgtgaa tgctcttttt tccagcgccg gcaagactgc ggccttcctg
    12181 gaagagaaca tcctctttga gaaggtgaaa ccgggcccaa aagccgacga aagcccgctc
    12241 ttgtttcctg ccgacgacca ccgccccccg cccttccagg tctggtgttg ggaatccgag
    12301 tccggcacgg tcacctccaa acaggccaag cgacagctgc ccacggcggt ggcggcggaa
    12361 gtttcccggc tgctcaatgc gaaggtccgc ctgggcgaaa acgccacgct tcggccacgg
    12421 gacattgcgg tcctggtgga atcccacaaa caggctcgct ggacccaggc agccctccat
    12481 gagctgcaca ttcccagcgt cgagcaggca atggagagcg tcctggagtc cggcgaagcc
    12541 cgcgaggtcc agtggattct ggcggcgatc ctagcaccgg gccgggaggc cgcggtcaaa
    12601 tcagcgctga cgaccgacgc actcggggtc agctgcgaca agctccaggc gctggtcgcc
    12661 aacgaatcca gctggcaggc gcgcttgcaa tcctttgccc ggtaccgcga cctgtgggag
    12721 gaggatgggt ttttcttcat gttttcgcag ctggtccggg aggaagaggt gatcgagaac
    12781 ctgctccgct tccccgatgc cgagcgccgg atcaccaacc tgctgcacgt tgccgaattg
    12841 ctggagaccg cctgcaaagc cgagcacctg gggccttcaa ggctcgtcca atggctggag
    12901 gaacgccgga tgagcgagga ggcgccaccc gatgcttacc agctccggct ggaaagcgat
    12961 gaggatgcag tgcagatcgt gaccatccat cgggccaagg ggctgcaata cccggtcgtg
    13021 ttttgcccgt ttgtcaccaa ggatgccaag ccgaggtcca tcaaggtgaa ccggcaaaag
    13081 gtgctggacg tcgtgctcta ccatgatccc gggagcaagc ggctgacctg ggacctcaac
    13141 gctgagccgg aagagaccca cgcgcaactg gccgccaagg aacaactggc ggaaaaagtg
    13201 cgcttactct atgtcgcgct cacgcgcgcc tgcaatcgct gctacctggt gagcgcgcgc
    13261 tatgcgcgga acaagtccac ggcgctggcc tggctgctgc gttcccaagg cggggagatg
    13321 ggcgaacaag tcgaccccgt taccgtcttg gataaggaga gtgttgttcc cagcggctgg
    13381 aaaagccggt ggaatgacat tgcttctgca tcggccgatc cagccggcgg caaaccgggc
    13441 attgcagtgg atgaccttcc gttggagccg ggagagccgt ggcagccgga gcagccggct
    13501 tccggaggat tgacccccaa gatctgtgtc agggagatca ggccttcctg gtttctctcc
    13561 agcttcaccc agctctccag ccacttatcc ctccggcatg ctcttgcttc agaaacggac
    13621 cacccggacc acgatgaggc tgcggccggg accctatccg tcggcactga ggatgaggca
    13681 accgagccac tgactggcat ttttgccctg ccagcgagtg ctcggacggg tgattgcctg
    13741 cacaagatta tcgagcgttt tgattttgcg gatgccgacg aagcaaccgc caaatccctg
    13801 gtgaggcagc agctcgaagc ctatgacctc tatgacgagg agcgagcccg ggctgtgatc
    13861 gggatgctgg agcggctgcg ccacaccccc ttggaccccg caaaccccaa tctcatactg
    13921 agcgcggttt ccagtgctga gcgactcacc gagatggaat tccattttcc cacgggccac
    13981 ttggacggcg cgcgtctgct ggacttgatt cgcagcccga atccctcgtc cgcaccggcc
    14041 tcctcccgcg ctgctgttcc cgtttcccgg attcaagcct ttctcaaggg gttcatcgac
    14101 ctggtcttcc ggtttgaagg ccgctaccac gtagtggact ggaaatcgaa tttgctcggc
    14161 accaaggtcg aagcctacac ccgggaggcg atgacgcgcg cagtcgagga gagctgctat
    14221 gacctccagt atcatattta cgtggtggcc ctcgacaagt acctgcggaa gcgggtgccg
    14281 ggctacgagt tcgaaacaca ttttggcgga gtgcattacg tgttcctgcg cggcttggct
    14341 cctgggcgtc cggacctagg cgtttaccac gatcggccgt ccgcccagaa gattgagtgt
    14401 ctttcgtcag ttctgggcac ctttgaggag gtgaaatcat gactcaagcc atttctccac
    14461 tgctttcggt tctggaaagc gacgctttca atctcacgga tcggcatttg gccaacctgc
    14521 ttgtaaagct ttcgggcaaa gagtgcccgt cgttgggcct ggcagcagcg ctggtaagtc
    14581 gcgagctcgc cgatggtcat tcttgcatca gcctggacgc gttcgggggg cgctcctttc
    14641 tggacagccg gccgggcaat ggggattcca tttgctgccc gccccgtgcc gaatgggaaa
    14701 ggctcctcct ggagacctcg gtcgtgggtc gtcccgggga ggacaagccc ctggtcctgg
    14761 atgaggccgg gcgtctgtac ctgcaccgtt actggcgcta cgaacaggca gtggcccagg
    14821 atctcctggc caggatcggg atggagtctc tcgcgcttga cctcaaaact ctcgccgctg
    14881 gcatgaatcg cttgttcccc ggcatccctg ggcaaatcaa ctggcagcaa gtgggggcct
    14941 ttgcggcagt gcgacagcgg ttttcggtta tcactggcgg tccgggcact ggcaagacct
    15001 ggaccgtcgc acgggtgctt gcattgctcc tggaacagcc agcgggccag aacctgaggg
    15061 tgaaactggc ggcgcccacc gggaaagcgg cagcccggct gcaggaatcc ctgggccagt
    15121 ccctcgagag cctggcgtgc tcggaggtga ttaaagcacg gctgcaggcc aaagatctga
    15181 cgaccaccat tcaccgcctc ctgggcccca tccccaattc ggccaggttc cagcacggcc
    15241 cggataaccc gttaccggtg gacgtgctgg tggtggacga ggccagcatg gtcagcctct
    15301 cgctcatggc tagacttctg gccgcgctca aaaaggagga cgtgcggctc gtgctggtgg
    15361 gcgacaagga ccaactgcca ccggtggacc ccggcggcgt cctgggggac atttgccggg
    15421 cggcttcggt gaacagcttc agcccctcgt tttgccaggc cttccagctc tgcaacggcg
    15481 tcgctctgcc cggcaagggg gccgtgctca acggagcgct ggcggataca gtggtgcaac
    15541 tggacgtgaa ttaccgttct ggcgaagccc tcgccctgca tacgctcagc acagcggtga
    15601 atgccggggc cacttcaaag gtcttggagc ttctgcggga cgccgacgcg ggtggcgccg
    15661 ttgtcgcctg gcaaagcctt ccgcagcggc cccggctcaa ggaagctctg cgggagaccg
    15721 tgcgttcgca ctaccgcctt gtgctgcagg ccgcttcccc gcaggaggct ttgcaagcgc
    15781 tgggccggtt tcgcatcctg tgcgcgctgc gggaaggacc ttacggcacg ctggccatca
    15841 atcgccttgt tgaagagatc ctggcggagg aacttcccgc tctggccgag aaaatgcgct
    15901 ttggcagcta cccgggaaaa ccggtgatgg tcacgtccaa taattacatt cttaaactct
    15961 ttaacggcga caccggggtt ttttggaccg ggaacggggg gtcctcgctg gtccacttcc
    16021 gcgacgagag cggctccgtc cgagctatcg cccgcgagcg attgccggaa catgaggtgg
    16081 tctacgccat gaccgtccac aagagccagg gatcggaatt cgagcgggtt ctgttgatgc
    16141 tcccggatag agaaaatccg gtgttaacgc gggagttgtt ttacacgggt ttaacgcggg
    16201 caaggaaatc ggttcggatc ctggccgatg agggcatttt gcgatctgcc atcgagacgc
    16261 gggcacaaag attctccggc ctgagcgatg ccctatgttc catgtcgcat cgtgcggttc
    16321 aactcgggct ctctccggtc cccgagagcg gtacgcctat agtacatata tgagccacat
    16381 cgcgctcttg ggcgactcca tctttgacaa cggagcttac gtgcggccgg ggcccgatgt
    16441 gattacccag ctgcgccgca aattgccggc aggctggcaa gccactctgt tcgctcggga
    16501 tggtgacgtc acatcgggag tcgcggggca attggaggtt ctgccagcga gcgccacgca
    16561 cctggtgatc agcgtgggtg gcaatgacgc cttgcaacac cagggagtgt tggcggaacg
    16621 catatgtttc gtcgccgagg cactccagaa gctgcgggcc atccaagttg agttttccag
    16681 atactaccgc tcaatgctga cggccgtgct tcactgcggg aagccgactg ttgtctgcac
    16741 catttacgat ggctgccacc ccgatatccg ggagcagcag gtcagggtgg ccgcgctggc
    16801 ccttttcaat gattgcatta ttcgggccgc tattgagcgg gagctgcctg tgctcgattt
    16861 gcgggctata tgcacccaag ccgccgatta cgcaaacccg atcgagcctg gggtggccgg
    16921 aggggaaaag atggctgctg cggttatcgg cgtggtgcag cgccacgatt tcacgggaaa
    16981 aagctctgcc atttaccagg gcgaggctcg ccccgcgaac ctgtgatgcc ccgaaccctt
    17041 gtggctatga gccgggcttt tcgctccagg cccgcacgaa gtcgtgctgg gatggaaatt
    17101 cggggctttc gggccggcgc ttccgttttt cctcactcat tgctccccag tgacgggcga
    17161 cttgcccaag tgcctgctgg ccgctgagcc cggagcgcac gagccaacag ccgacgaccg
    17221 ttcccgtccg gcccacgccg ccccagcaat gcacatagac cacgtgccct tgggccagtg
    17281 cattttggat cgtgtccagt atctgtgtca tttccgatct ggccggaacg gaggtgtcct
    17341 ttatcggtat gcgctggtaa ttggtttgtt tcccggcaac ggtggctgcg ccgaggatct
    17401 ggtcatagcg tttgaggctg tattcaccgg gttcggttag gtccaggaag aaggtgatcc
    17461 cgcaatccag cgttcgctgg atcttagccc tggctttcgc gtcggctttg tcgcccgggt
    17521 attcgccagc catgaggcga tcggattcca cccagtaaca attcccgtga ggacggttgc
    17581 cgaaacggta aagcccggac gcaagatatt ctatgatctg ccgcttgaaa cggtcctgtt
    17641 tgtagaactg aatacaggcg aaactggtag cacttaggta agatagggtg gccaaactca
    17701 agaaactgaa aagagcaaag gggggaaact cgaacaatcg ggggaatcgg cgaatcatcg
    17761 tccacaacaa cgcttgtatt tctctccggt gccgcaagga cagtagtcgt tcctcgccac
    17821 tctccgagac ggcgtttgtt gactgcaaga tctttggccc ttgggcggag tcgttggcca
    17881 agcacaagaa aggagacatg gccctcgtgt ccgggcgcct caaaactgac tcgtgggaaa
    17941 aggacggccg gaaacaatct aagctcattg tgatctgcga ttccgttcgg ttcttcgtgc
    18001 cgcaaaatgg ttcgtccgcg tcggccagtg tggccgaaaa cgaatcggca atgcaggagg
    18061 agttgaacga caagaatggc aagcctccgt tctgacggca acgagaagga gcgtcagcgg
    18121 ccgaccattc aatgaaacga tcggccgctt cggctacgcc gaaagaatct gtccgccatg
    18181 ataaatcacc tgaatcattc agaactcgaa gatcctaaat tgaactcccg atgggaggcc
    18241 gtgcgtgaac cagcgtggga atgcaatcag actgtcttcg cggatttcca ccgccgacat
    18301 ggttatcgcc gcaacgtcct caactgcgcc attccgttcg cccgagccaa gacgctgacc
    18361 gacttcacca tgccgcaagg ctggaacctc gacaaatccc tcatcgccag cgtcggcttc
    18421 ggcgccaagc tgccggccgg cctcgaaaac tggagttaca cgccaaagat ccaagccgtc
    18481 aagcaactcc cccagatgaa ggatctcccg cgggagcaca gcaccctgat gcactctccc
    18541 tggttccagt ccccgtccca ggcgatgggc ctcggcctca acgcccgcca cctgcgctac
    18601 cactacgagc tctggaacga catccagccg aagaccctgc gcggcgtatg cacgaccctt
    18661 gtctacgtca ccccgcagat cgggttcgcc ctgacgcccc acatcaaatt ggaccgggtt
    18721 ttccgctact tcggggcgct ctcccggatg gccggcccca cccagttgtt cccagcctcc
    18781 tacctctacc cggatgcccg gctccacggc ttggagaagg tgcctgcggc tatgttcgcg
    18841 gacatcctcc cacaccttgg ctccagcttc ccgctgatgg acgaatccac caacccctgg
    18901 gacgtggtcg tccgcctcaa cggccccgac tgctactacg gctacccgct ctcccgggtc
    18961 tccaacccgg tggaggacgt caccctcagc aacgcgaccg ccgggagcat gacgcttccc
    19021 aacggccagc aggtcaaggc ccgcagctct gagacgttct gctatgcgac ccttgacccc
    19081 aaactcaagg acgccctcaa gatggatcgc gacgcattcc acgccgccta ctgcctcgac
    19141 cgcggggtca tcacccatga cggcctcccg ttcatgctgc cggtggagat gcagtcggcc
    19201 gtgttcgccg tcatcctcac cgcctgggag ctggaaatgg acaagcccgg cgacctcacg
    19261 tcctacaccc tcaagaccca gcactccgcc atccggggct acaacttcta ccagaagatc
    19321 gagccctact actcctgctt catgcggctg gtgcatgatc tttcccaaga tcaaaacttc
    19381 gaaacctacc cattgtccag ccatctgacg cattcctggc tcggcggcaa cctcctgctc
    19441 tccaatcagc ggtccgaggc cgaagaggtg caggggatca atttcctgcg caactactac
    19501 ctgcagcagc tcgactggtc catgggcgtt tacaatttgt ggaataccgt cgagtttcgc
    19561 cgtggcagcg acctcgagag ccagttggat cacgccggtg tggcatttca tcagatgagg
    19621 cctcagttgc gccacgatgt gcacctgttc caacgccccc tgcgcagggc gtccttctgg
    19681 ccggacttca accccaagtg cctggagttt gaccaaacct acatggcgga agggcttcca
    19741 acgccccatg agcggtaaat ccatagtcga gatatagatc atcacccaac aaagaattca
    19801 gattaacaac aataagaaaa ccacctaatg aacactgaat caaacacctc accccagaac
    19861 ctcatcgtgt gccagttcac acgtgattgc atcgagaagc agcaacttgc ccctttcttg
    19921 gattattggg cgccccacaa attaccacac ggtccagcgc ttcggacgct gatgaacaaa
    19981 atgttttttc acattgaggg ttacgatggc ccgcgcgcgg tctgcacgat tccggaggtc
    20041 cggtcatttt gccaggcgtt tttcgagagc tggccttttt ggttttttgt ggcgcacctg
    20101 gatagtccga gcttgctcat tatggtgttc gcctgcctgc agaagttgcg cgtggtcgac
    20161 gaggaggcag gtggggtctg tcatgtgcaa atcgacgcag tagagctgaa cgaattcatt
    20221 gagcgcggtt tggttggcat ggaatgtctt tgcaaacgtg ctggcatgag cccagcagaa
    20281 atctcgctac gatccatgca aatcatcgag tatttccaaa ccgactggag cagcagttcg
    20341 gcccaagcag ccgacggcaa accggaaacc gggaggtgga attgaggagc aggacatctg
    20401 gctcccgatt cgaaaaccca agaattgttg ccaactagtc cgctcgtccg gttcggttga
    20461 tgcacgcctg aaggcgcaag aagagccggc agaacccaag aaacccaatc tttaggcggt
    20521 tcagctcacc aacgcaaatt acaccagcaa taactcacac tatgaacagc tacaaaaaca
    20581 aagcaccgat caatcacaac ccttcctccg cgaagaagcc ggtgttccgt ttcaattggc
    20641 ggggaaaccg accgcgaatg gggaaacgcg ggatgtgtcg tcggtggtgc tgagaaagga
    20701 gccatgccag tctgtgcaat cagatccacc agcttgaccg aaaggctgga aatctatcgg
    20761 gccaccagca acagataccg ccaatggtat gcggatcagg aagagcatgg gggccggtgt
    20821 ttctactggt ccctcacctt gatgggtgtg ctgctcgaaa acgggtatcg cgccctcatt
    20881 caagccggca gcatgtcttg gccaatcgtt ccaccgggtc aggacgacgg cagaagcccg
    20941 acgcacttct cttacgagtg gtcaccctgg cgtgaggaga gccaggccgc gttgaagctg
    21001 ggtctgctgc cggagattca cgtttgggtt ggcttgcctg accagaacga gttgctggac
    21061 ttcgccacaa agttcctgcc cgagcaagct gcaaaggagg gattggcctg gcgcacacca
    21121 aatccgccag acttcctctg gtgcggcccg tccgagctgc cagagggagt cctgtataaa
    21181 ccggacctca acgcgatcgg gttcatcctc gatcgaatca gcaaaaccaa ccaccgctag
    21241 aacggtgaca agaaaggaga acaatgccga attggtgcct aaacaagctc accataaccg
    21301 gaccagaggc ggatgttcag gcgtttaaga caaaagccgt tggacattcg ccttgggagg
    21361 agcccgaggg ggagcctgac gcgctcaact tccacagcct ggtccccatt cctgagaatc
    21421 tactggcggc cggttatgac ggcgctagat acgattggga gatggcaaac tggggctgca
    21481 aatggggggc ttctggtccg gggattctcg acgagaggga aggtcatgtg gaatacgaat
    21541 tctacgccgt gctgagggcc agccaggttc cgctagccgc aggcgtatcg gccccgagcg
    21601 gcgagaacgg accgcctccc caccaggtca tcaagctggg catcgacgtg catctggatc
    21661 gctacgtggt ggtgcgccaa atcgatggcg gtgcgcccca gccgccgcaa cgattcagcc
    21721 caacccagtt tctggagtgg gccaagaaac agacggcgct ggcccagcag gtttacagtt
    21781 gttacgaggc gggccccttt ggttaccgtt tgcatcggaa gctcaaggag ttgggcatta
    21841 ccaactacgt gatccggccc cgggattggg acgagtatgg caagaaggtc aagaccgaca
    21901 agcgggacgc caaagagatg gtgctgaacc tggaccggta tgtctccggc aatcaggagg
    21961 cgttctgcgt cgtgcgcgtg cccacggaag ccgaagaaca agcccggagc cgctcgcgcc
    22021 agcgggagag ctggcaaaag gagaagcaac gtctggcggc ccaggggcgc agccacgcct
    22081 tgtattacgg cgcgcatctg gagggtgcct ggtggaccgg aggtctctgg aaggagatgg
    22141 tggtgccccc cattgtgctg gagttgctgg agccgttgcg ccggctcatc caggcgctgg
    22201 agcaggaact caagaccttg acccaggcgc tgaccaccgc caccccgaag cagctaccga
    22261 tgggtttggg taaactgacc tatgaagtgt tggagcgaga agtgggggac tggcaccggt
    22321 tccacaatcg gcggcaagtg gccagttaca ccggaatgtg tccccgggaa gacagctcgg
    22381 cccagcggcg ctttcaaggg tccatcaaca agcacggcaa cccgcgcatg cgcaccgtcc
    22441 tggtggaggc ctcctggcgg ctggtgcaat accagcccac ttacaagccg gtggcccggt
    22501 ggctgccggt gctgacccat cccaagacca cccggagcaa gcgcaagcag atcattgtgg
    22561 cgattggccg gcagttcggc gtggactggt ggcgcgtgcg cacgcggcgc tgccaagc
//
LOCUS       DATCGS010000166         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_65154, whole genome shotgun sequence.
ACCESSION   DATCGS010000166 DATCGS010000000
VERSION     DATCGS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65154"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VIL39_03345"
     CDS             <1..1294
                     /locus_tag="VIL39_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5480491.1"
                     /translation="FLLINHPLDCPICDQAGECKLQEYSVDYGQSESRFAETKVHKPK
                     RVDLGPRIVLDDERCILCTRCIRFTRDIVGDDALGIVNRGSYNTLTAYPGRVFDNNYT
                     LNTVDICPVGALTSKDFRFQMRVWFLKETKSLCTSCATGCNIVIGSREEKIYRYEPRE
                     NDAVNACWMCDYGRLNYKWIGRQDRLGKVQSPKSKVQSQPQGWTAVLKELSEKLKQAP
                     AGSVAVVASARQTNEELYLLAKLAKTLGALTDSVPRAGEGDRLLLNADRNPNSNGAQL
                     TGIAAEPMGSKLPKIAEGIRSGRIKTLIVFGEDVTRHGLGADLLGKLETLIVSDILPN
                     ETTRLAHYLLPGCAHAEKRGTFTNTKGRVQKFMKAVEPPGDARPEWEFLRELALNTTG
                     ENGVVSIEALFNQMAAEIPAFNGLTWAGLGDLGATVRI"
     gene            1298..2404
                     /locus_tag="VIL39_03350"
     CDS             1298..2404
                     /locus_tag="VIL39_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY5480492.1"
                     /translation="MIQAIIDWFGSPAGQSVLFSAVKILGVFSVLMFIVAYAVWVERK
                     VAAAIQDRRGPNRVGPFGLLQPAADAIKSFLKEDFTPAHVRKTYYWLAPAIVMIPSLV
                     TVAIIPFGSLLGSQKMVIADLNVGVLYTFGIVSLGVYGIVLAGYAARSKYPFIGGIRA
                     SAQLISYEIAMGMSVIPLFLIAGGLDVQQLSAGFQSLLAGTSGLNLNRLVTWQAANGW
                     LIFYAPFSFIIFLAAMFAETNRMPFDMPESEQELAGGYNVEYSSMKFALFFMGEYANM
                     TAAAALMVTLFLGGWSLPVTGLNQPAGTMWAGLLQIGIFLAKVCCVLFMVIWVRWMWP
                     RFRYDQLMDLGWRRFIPLALANIVVTAVVLWAKS"
     gene            2405..>2610
                     /locus_tag="VIL39_03355"
     CDS             2405..>2610
                     /locus_tag="VIL39_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5480493.1"
                     /translation="MIIKRRKLNLWERLYLPAIIGGFTVTLRHFFKKKVTMQYPEERW
                     VVPEGYRGAPYLVKDQDGRTKCVS"
BASE COUNT          503 a          729 c          823 g          555 t
ORIGIN      
        1 atttctgctc atcaatcatc cgctcgactg cccgatctgc gaccaggccg gggaatgtaa
       61 gctgcaagaa tactccgtgg attacgggca atccgagagc cggtttgccg aaacgaaggt
      121 tcacaagccc aagcgcgttg acctggggcc gcgcatcgtg ctggacgacg aacgctgtat
      181 tctctgtacg cgctgcatcc gttttacgcg agacatcgtg ggcgatgatg cgctcggcat
      241 cgtgaaccgc ggcagctaca acacgctcac ggcctatccc ggcagagtgt tcgacaacaa
      301 ttataccctg aacacggtgg acatctgccc ggtcggcgcg cttacctcga aggatttccg
      361 attccagatg cgcgtctggt tcctgaagga gaccaagagc ctttgcacga gctgcgcgac
      421 ggggtgcaac atcgtcatcg ggtcgcggga ggaaaagatc tatcgctatg agccgcgcga
      481 gaacgacgcg gtcaatgcct gctggatgtg tgactacggc cggctgaact acaagtggat
      541 tggacggcaa gaccggctcg ggaaggtcca aagtccaaag tccaaagtcc aaagtcagcc
      601 gcaagggtgg actgcggtct tgaaggaact ctcagagaag ctgaagcagg ctccggcggg
      661 gtcggtcgcg gtcgttgcct cggcccggca gaccaatgag gagctttacc tgctggcgaa
      721 gctggcaaag acgctgggcg cgttgacgga ttcggtgcct cgcgcgggag aaggcgaccg
      781 gttgctgttg aatgcggatc gtaatccgaa cagcaacggc gcgcagctca ccggcatcgc
      841 cgccgagccg atgggctcaa agttgccgaa gatcgccgaa ggcatccgca gcgggcgcat
      901 caagaccttg attgtttttg gcgaagacgt gacccgccat ggcctgggcg cggacctgct
      961 cggcaaactg gagacgctca ttgtgagtga tattctgccg aacgaaacga cccgactggc
     1021 gcattacctt ttgcccggct gcgctcatgc cgagaaacgc ggcacgttca ccaacaccaa
     1081 agggcgcgtt cagaagttta tgaaggcggt tgagcctccc ggggacgccc gaccggaatg
     1141 ggaattcttg cgggaacttg ctctcaatac aacgggcgag aatggtgttg tgagcattga
     1201 agcgttgttc aaccagatgg ctgccgagat ccccgcgttc aacggattaa cgtgggcggg
     1261 ccttggcgac ttgggcgcga cggtcaggat ttaggcgatg attcaggcga tcatagattg
     1321 gtttggaagc ccggcggggc agtccgtgct gttcagcgcg gtgaagattc tcggcgtctt
     1381 ctccgtgctc atgttcattg tcgcctacgc ggtctgggtc gaacgcaagg ttgccgccgc
     1441 cattcaggac cgccgcggcc ccaatcgggt tgggccgttc gggctgctcc aaccggccgc
     1501 cgatgccatc aaatcgttcc tgaaagaaga ttttaccccg gcccatgtcc gcaagaccta
     1561 ttactggctg gccccggcaa tcgtgatgat tccaagcctg gtcaccgtgg ccatcatccc
     1621 gtttggctcg ctgctcggca gccagaagat ggtcatcgca gacctgaacg tgggggtcct
     1681 ttataccttt ggcatcgtct cgctcggggt gtacggcatc gtgcttgcgg ggtatgcggc
     1741 cagatcgaag taccctttca tcggtggcat ccgcgccagc gcgcagttga tttcctacga
     1801 aatcgctatg ggcatgtcgg ttatcccgct gttcctgatc gccggcgggt tggatgtcca
     1861 gcagttgagc gcgggattcc agagtttgct cgcagggacg agtggtctta acctgaaccg
     1921 cctggtcacc tggcaggccg ccaacggctg gctcattttc tacgcgccgt tctcgtttat
     1981 catcttcctg gccgccatgt ttgctgagac gaaccgcatg cccttcgaca tgcccgagtc
     2041 ggagcaggaa ctggccgggg gctacaacgt cgaatacagc tcgatgaagt tcgcgctgtt
     2101 cttcatgggc gaatacgcca acatgaccgc ggccgccgcg ttgatggtga cgctttttct
     2161 gggtggctgg agcttgcccg tgaccggctt gaaccagccc gcggggacga tgtgggccgg
     2221 actgcttcaa atcggcatct tcctggcgaa agtgtgctgt gtcttgttca tggtgatctg
     2281 ggtgcgctgg atgtggccgc gcttccgcta tgatcaattg atggatttgg gctggcggcg
     2341 ctttattccg ctggcgctgg caaacatagt ggttacggcc gtggtccttt gggcgaagag
     2401 ttagatgatt atcaagcgcc gaaaactgaa tctttgggaa cgcctttacc tgccggcgat
     2461 catcggcggg ttcacggtca ccctgcgtca tttcttcaag aagaaggtga cgatgcagta
     2521 tcccgaagag aggtgggtgg tgcccgaggg ctatcgcggc gccccttacc tggtgaagga
     2581 ccaggacggc cgcaccaagt gcgtttcgtg
//
LOCUS       DATCGS010000167         7109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_164650, whole genome shotgun sequence.
ACCESSION   DATCGS010000167 DATCGS010000000
VERSION     DATCGS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164650"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIL39_03360"
     CDS             complement(<1..709)
                     /locus_tag="VIL39_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY5480494.1"
                     /translation="MNKTLVVVPTYNERENLPPLAQRLLALPVPVDILVVDDNSPDGT
                     GKLADELAAKHPTIHVLHRTEKSGLGRAYIAGFKWALEHSYEFVFELDGDFSHNPDDI
                     PMFLEAAKNADLVLGSRYLNGIRIINWPLSRLMLSKSAAKYVQVITGMKITDPTGGYK
                     CFSRRALEAINLEEIRSNGYSFQIEMTHRLWRQGMRVVEVPIIFTERAQGHSKMSGHI
                     IQEALIMVWRLWFQNKMR"
     gene            1075..1165
                     /locus_tag="VIL39_03365"
     tRNA            1075..1165
                     /locus_tag="VIL39_03365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1109..1111,aa:Ser,seq:gga)
     gene            complement(1245..2276)
                     /locus_tag="VIL39_03370"
     CDS             complement(1245..2276)
                     /locus_tag="VIL39_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5480495.1"
                     /translation="MAENCFHPSIEGAQHGAKSLTQFLDYAKKSGASGAQPSNFMLNA
                     EKGLKSAKEIKDAFAKAKLSLDGISAHCPFWVHTTAWTGSPTIRPFTPADVAKKSPEE
                     IEKWAEDYILRLLDLSAELGVKIVPMFWGVAFGWELATGYPWGFWKGGDYDFLKEGQD
                     RFVKKTAKIRKHAKSLGIYLAHEIHPGTAAMCADDFNMLVEICGGDKCLTVNADPSHC
                     WEGEGWETRFLKVAPRIYACHVKNYVIRQGLPLRMMEASWQKRAMQFVDIPSGDLNMT
                     RYVELLIHTGYPQRYCQVMGTKTAPLIVEAESAYRDLDVTSANGIQYVRDNLCFPIAA
                     GSFEDGMGA"
     gene            2412..3230
                     /locus_tag="VIL39_03375"
     CDS             2412..3230
                     /locus_tag="VIL39_03375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5480496.1"
                     /translation="MPMFYDTHAHLNYPDFAEELPAVIARAEAAGIAKIISVGTDLAS
                     SVRAIKLAEEFPNVFAVVGWHPSHALEAPDDLRPALREMARHPKVVALGETGMDYYRL
                     PSRKPGLTAADDETYKTKQAALFRQHLEVAAELGLNCVIHQREALEDTLAALQPFADR
                     VRGVFHCFANDAATMRRIVALGSLVSFTGILTFKNGQNIRDALAATPMDQFMLETDCP
                     FLAPMPYRGKRCEPAYVKETAAVAAQVKGCSLDELSAATCATAEKFFAKLSARP"
     gene            complement(3520..3936)
                     /locus_tag="VIL39_03380"
     CDS             complement(3520..3936)
                     /locus_tag="VIL39_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY5480497.1"
                     /translation="MDARLKAEVRQRAGARCEYCQFPEAFAELRFQVDHIIPQQHGGP
                     TASANLAWSCLRCNKHKGPNLSGIDPATGQVVRLFHPRQDRWAEHFVWEATRLLGLTP
                     VGRTTAAVLQINRTDAVLVREALMEEGVYLAKDSKG"
     gene            complement(3946..4230)
                     /locus_tag="VIL39_03385"
     CDS             complement(3946..4230)
                     /locus_tag="VIL39_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480498.1"
                     /translation="MNAVIADHSPVAIWKRTIQPERASFAPAEARAILRLRLSSLDLG
                     RADELAAKARAGGLAAEEEREMDDYVAIGGALEFLKSKARLSLKRARAAA"
     gene            complement(4406..>7109)
                     /locus_tag="VIL39_03390"
     CDS             complement(4406..>7109)
                     /locus_tag="VIL39_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5480499.1"
                     /translation="FPANAPQVSALLDAAVQASITVIPRGAGTGLAGGAIGDGLVIDF
                     SRHNRAITDLDLERRTVRVGPGVVLDNLNKFLHPHGFCFGPDVATSSRATLGGMIANN
                     SSGSHTPVYGTTADHLNELEIVLADGRAVEVGPAHDTLRKQRELVGDLVFLHGLTIAE
                     RLAPGLLKRWPGYGIDRCVREPDNLNHLLCGSEGTLAAITSAELKIVPLPKQKGLALI
                     FFASVAEAMQATVELLDLKPAAIEHLDRVLLDQTQGQREFQPARDLLELEFKPCQSIL
                     AVEFFDEVDEPLAALAKRKLGLRKMILKTQAEANLIWALRRAGLSLMTGRKGAAKPTT
                     GIEDVAVRPKDLPAYVAGLQSLMAPLGLQASYYGHAAAGLLHVRPILDLHSAEDLKKF
                     RQLANEVSALVARFKGSLTGEHGVGIARSEFMAEQLGDGLLGVMRDIKASFDPNNLFN
                     PGKVVPDGRFAIDTELRLGAGSELKLPFTPILAFAAKDGSFTRNLEQCNGCGGCRKET
                     PGMCPTFIATGEEIMSTRGRANAIRAALELRGLEKGDPLRCAELEAALSNCLSCKACT
                     TECPSNVNMTLLKAELLHARIRRDGLSLRERLVSSVDLLGKLGCCMPRLANLALDSLF
                     VRSILAKTLGIAWQRPLPHYARQRFDHWFKRHQPSPKAPRGRVVLWDDTFVRYHEPNI
                     GIAAVKVLEAAGFEVGLPVGRKCCGRPAFSQGNLDAAARLGQHNLALLNLDVDGAPIL
                     FLEPSCYSMFVEDYRELKLEGTEHVAQRCFLFEQFIEELLAHEPNALKFNAKAANVVI
                     HAHCHAKALMNPAFMRKLAERLPQRNVTLLDTGCCGMAGAFGALESKYELSLKVAEPL
                     AQKVRGQPFGTIVVASGTSCRHQIDHLAPVRARHMAELLADALV"
BASE COUNT         1317 a         2148 c         2299 g         1345 t
ORIGIN      
        1 gccgcatctt gttttggaac cagagccgcc agaccatgat taaggcttcc tggatgatgt
       61 ggcccgacat cttggaatgg ccttgagccc gctcggtaaa gatgatcggc acctccacga
      121 cccgcatgcc ctgccgccac agccggtggg tcatctcgat ctggaagctg taaccattgg
      181 agcggatttc ctcgaggttg atggcctcaa gcgcccgtcg gctgaagcat ttgtagccgc
      241 ccgtggggtc ggtgatcttc atgccggtga tgacctggac atactttgcc gcagacttgc
      301 tgagcatcag gcggctgagc ggccagttga tgatgcggat gccgttcaga taccgcgagc
      361 ccagcaccag gtcggcattc ttggccgctt cgaggaacat ggggatgtcg tccgggttat
      421 gcgagaaatc tccgtccagc tcgaacacaa actcgtagct gtgctccagc gcccatttga
      481 agcccgcgat gtaagcccgt cccaatccgc tcttttccgt gcgatgcaac acgtggatgg
      541 tggggtgttt cgcggccagc tcgtcggcga gcttgccggt gccatcgggc gaattgtcat
      601 ccaccaccag tatgtccacg ggtacgggca gagcgagaag gcgttgtgca agcgggggaa
      661 ggttttcccg ctcattgtag gtcggcacaa cgacaagggt cttgttcatg caattaatgc
      721 ccggggacaa ataagcgacc tgccgaacga aaacaagcgg gaattctgct atcgcgggta
      781 ctcgggggag atggcctggg cggggtgcgc ccatcctcgg gcgcagcaag gccggaaagc
      841 gtcggagctt tgatgaaatg gcgaccgtgg gcacacgtga tgcttgcggc gcccgatggt
      901 aagggttgac tttatttggc cggtttggcc aaggccgggg acaaggtgta gcgcagacgt
      961 ctcgccggcg agaacgcctg cgctactttg tcaacgcccc tgggtttggc cggttgcgcg
     1021 aggaaaaaca ttgcgtaaca ttagcctcgc ctccacagtg ggctttgcct ggccggagag
     1081 atggctgagt ggtttaaggc acacgcctgg aaagcgtgcg tgggcttata ccccaccctg
     1141 ggttcaaatc ccagtctctc cgccagcttt aaaaaaatgc ccctccccaa caatcgctgg
     1201 agaggggtga aggcggatac cccgtaatac cgaatcggca ggagctacgc gcccatgccg
     1261 tcctcgaacg agccggcggc aatcgggaag cagaggttgt ctctcacgta ctgaatgcca
     1321 ttggcgctgg tgacgtccaa atcgcggtag gcgctctcgg cctcgacgat cagcggcgcg
     1381 gtcttcgtgc ccattacctg gcaatagcgt tgcggatacc ccgtgtggat cagcagctcg
     1441 acgtagcgcg tcatgttcag gtcgcccgag gggatatcaa cgaactgcat cgcgcgcttt
     1501 tgccaactgg cttccatcat gcgcagcggc aggccctggc ggatgacgta gttcttcacg
     1561 tggcaggcat agatgcgggg ggcgacttta aggaagcgcg tctcccagcc ttcaccttcc
     1621 cagcagtggg acgggtcggc gttcaccgtc aggcatttgt ccccgccgca aatctcgacc
     1681 agcatgttga agtcgtcggc gcacatggcc gcggtgccgg gatggatttc gtgggcgagg
     1741 tagatgccga gcgatttggc gtgcttgcgg atcttggcgg tcttcttgac gaagcggtct
     1801 tgtccctctt tcaggaagtc gtagtcgccg cccttccaga atccccacgg ataacccgtg
     1861 gcgagctccc agccgaacgc gacgccccag aacatgggga cgatcttgac gccgagttca
     1921 gcggagagat cgagcaagcg caggatgtaa tcctccgccc acttctcgat ctcctccggc
     1981 gacttcttcg ccacatcggc gggggtgaac ggccggatgg ttgggctgcc ggtccaggcg
     2041 gtggtgtgga cccagaaagg gcagtgagca gaaatgccgt cgaggctgag cttggctttc
     2101 gcgaaggcat ccttgatctc cttggcggat ttgaggccct tctcggcgtt gagcatgaag
     2161 ttggagggct gcgcgccgga tgcgccggac tttttggcgt aatccaggaa ctgcgttaga
     2221 ctcttcgcgc catgttgggc gccttcaata ctgggatgga aacaattttc ggccataatg
     2281 ttttaggaag ttaaacagtt ttattcattt accgaccggc aaaaggattg ccgcccccgg
     2341 cgacggaacg caaccttaaa tttcagcgcc ttgaagggaa cggggccggc tggtaatgtg
     2401 atggcgctat tatgccgatg ttctacgaca cccacgccca tcttaattat cccgactttg
     2461 cggaggagct gccggcggtg atcgcccggg ccgaagcggc gggcattgcc aagatcatca
     2521 gcgtcggcac ggacctcgcc agcagcgtgc gcgcaatcaa gctggcagag gaattcccca
     2581 atgtgttcgc ggtggtgggc tggcacccct cccatgccct ggaagcgccg gatgatctgc
     2641 ggccggcgtt gcgtgaaatg gcgcggcatc ccaaggtcgt cgcgctcggg gagacgggaa
     2701 tggactatta ccggctgccg agccgcaagc ccgggctcac cgccgccgat gacgagacct
     2761 acaagacgaa acaggccgcg ctatttcggc agcatctgga ggtggccgcc gagctgggac
     2821 tgaactgcgt gattcatcag cgcgaggcgt tggaggacac gctggcggcg ttgcaaccgt
     2881 tcgcggaccg ggtgcgcggg gtattccatt gcttcgccaa cgatgccgcg accatgcgac
     2941 ggatcgtcgc gcttgggtcg ctggtcagct tcaccggcat tttgacgttc aagaacgggc
     3001 agaatatccg tgacgcgctg gccgcgacgc ctatggacca gttcatgttg gaaacggact
     3061 gcccgtttct cgcgccgatg ccgtaccggg gcaaacgctg cgagccggcc tatgtgaagg
     3121 agacggcggc cgtcgccgcc caggtgaagg gttgttctct cgacgagttg agcgccgcca
     3181 cctgcgcgac ggcggaaaag ttcttcgcca aactatcggc gaggccgtaa cgggaacatc
     3241 cagcgccgta tctttaccaa accgccatct ggcgcttggc ggcgcttgac tgaagcttag
     3301 cgaagaagct ctgaccgctg cttgggagaa ccccgacgac gacgttttca atgagcttct
     3361 ccagaggtga agtcgtcctg ctcccgatcc cttttacgcg tatggctcta ccgatgattg
     3421 tggatttgcg atttgcgatt tactatttgc gattggcgac tgacggcgcg tggtgaaggc
     3481 cgccgcccaa tcgccaatca agaatccaag atcgaagcgt caacccttgg agtccttggc
     3541 gagatagacg ccttcttcca tcagcgcttc gcggaccaat actgcatccg tccggttaat
     3601 ctgaaggaca gcggctgtgg tccggcccac gggggtgagt cccaagagcc tggttgcctc
     3661 ccagacaaag tgttcggccc agcggtcctg gcggggatga aacaggcgaa ccacctggcc
     3721 ggtggccggg tcaatcccgg aaaggttcgg gcccttgtgt ttgttgcagc gcaggcacga
     3781 ccacgcgagg ttggcggaag ccgtcggccc gccgtgctgc tgagggataa tgtgatcgac
     3841 ttggaaccgc agttcagcga aggcctccgg gaactggcaa tactcacaac gagcccccgc
     3901 ccgctgccgc acctctgcct tgagccgggc atccaccagc ggctgtcagg cggcggcccg
     3961 cgcgcgtttc agcgacaagc gggctttgga cttaagaaac tccagcgcgc cgccgattgc
     4021 cacgtagtcg tccatttccc gctcttcctc agcggccaat ccacccgccc gtgccttggc
     4081 ggccagttca tcggcccggc caaggtccag cgaggaaagc ctcaggcgca agatggcccg
     4141 ggcctcggcg ggcgcgaaac tcgccctctc tggctggatg gtcctcttcc aaatggcgac
     4201 cggggaatga tcggcaatta cggcattcat ctccccttac tttggcatgt tctcggcata
     4261 tttcaagccc aaaaaccgca gcccgaaaac cgcaggacgc gtcttggcaa ttggcggccc
     4321 cgcttcctga gcttcgtaga acaggaatcc atcgttaagg tcctggagtg cgtcttcaac
     4381 gaggcgtaaa acagccgggt tagacttata ccagtgcgtc ggctagcagc tcagccatgt
     4441 gccgggcccg cacgggggcc aggtggtcaa tttgatggcg gcaactggtg ccggaggcca
     4501 ccacaatggt tccgaagggc tggccccgaa ccttttgggc gaggggctcg gcgactttca
     4561 gcgagagctc gtacttggat tccagggcgc cgaaggcacc cgccatgccg cagcagccgg
     4621 tgtccagcag ggtgacattg cgttgcggca ggcgctcggc gagcttacgc ataaaggcag
     4681 gattcatcag tgccttggcg tggcaatggg cgtggatgac gacgttcgcg gctttggcgt
     4741 tgaacttgag cgcattcggc tcatgggcga gcaattcctc aatgaactgc tcgaagagga
     4801 agcagcgctg ggcgacgtgc tccgtgccct ccagtttgag ctcgcggtaa tcttccacga
     4861 acatggaata gcaggagggc tccaggaaga ggatcggcgc gccgtccacg tccaggttca
     4921 gcaaggccag gttgtgctgg ccgagccgcg ccgccgcgtc gaggttgccc tggctgaagg
     4981 ctggccggcc gcaacacttc cggccaaccg gcaagcccac ttcgaacccg gcggcttcca
     5041 ggaccttcac cgcggcgatg ccgatattgg gctcatggta gcgcacaaag gtgtcgtccc
     5101 agagcacgac tcggccgcgc ggggccttcg gcgagggctg gtggcgcttg aaccaatggt
     5161 cgaagcgttg ccgggcgtag tggggcaggg gacgctgcca ggcgatgccg agcgttttcg
     5221 ccagaatgct gcgcacgaag agcgagtcta acgccaggtt ggccagccgg ggcatgcagc
     5281 agccgagctt gcccagcaaa tccactgagc tgaccagccg ctcgcgcaag gacaagccgt
     5341 cgcggcgaat ccgcgcgtgc aggagttcgg ccttgagcag cgtcatgttg acgttcgagg
     5401 ggcattcggt ggtgcaggct ttgcaggaga ggcaattgct gagcgcggct tccagctcgg
     5461 cgcagcgcag ggggtcgcct ttctccaggc cgcgcagctc gagcgcggcg cgaatcgcgt
     5521 tggcccgccc gcgggtgctc atgatttcct cgccggtggc gatgaacgtc gggcacatcc
     5581 cgggcgtttc tttgcggcag ccgccgcacc cgttgcactg ctccaggttg cgggtgaacg
     5641 agccgtcttt cgctgcaaag gccaggatgg gcgtaaaggg cagcttgagt tcgctgccgg
     5701 cccccaggcg caattccgtg tcaatcgcga agcgcccatc gggaacgacc ttgcccggat
     5761 tgaacaggtt attcggatcg aacgaagctt tgatatcgcg catcacgccc agcaggccgt
     5821 cgccaagctg ctcggccatg aactcgctcc gggcgatgcc gacgccgtgt tcgccggtca
     5881 gcgagccctt gaagcgggcg accagggcgg acacctcatt cgcgagctgg cggaacttct
     5941 tcaggtcctc ggcggagtgc aggtcgagga tcgggcgcac atgcagcaag ccggccgccg
     6001 cgtggccata atacgacgcc tgcaaaccca gcggcgccat gagcgattgc agcccggcga
     6061 cgtacgcggg caggtccttc gggcgcacgg cgacatcctc aatgcctgtc gtgggtttag
     6121 cggcgccctt gcggccggtc atcagcgaaa gtcccgcgcg gcgcaaggcc cagatcaggt
     6181 tcgcctccgc ctgggtcttg agaatcatct tgcgcaagcc cagcttgcgc ttcgccagcg
     6241 cggccagggg ctcatccacc tcgtcaaaga actcgactgc gaggatggat tggcagggct
     6301 tgaactccag ttcgagcaga tcgcgggcgg gttggaactc gcgttggccc tgggtctggt
     6361 cgagcaacac ccggtcgagg tgctcgatgg cggcgggctt cagatcgagc agttccaccg
     6421 tcgcctgcat cgcctcggcc accgaagcga agaagatcaa ggccaggccc ttctgcttcg
     6481 gcagcggaac gatcttcagc tccgccgagg tgatggcggc cagcgtccct tcgctgccgc
     6541 agagaagatg gttgagattg tccggctccc gcacgcaacg atcaatgccg taaccgggcc
     6601 agcgtttcag cagacccggc gccaggcgct cggcaatcgt cagcccgtga agaaacacca
     6661 ggtcgccgac cagctcacgc tgcttgcgca aggtgtcgtg cgcggggcca acttccacgg
     6721 cccggccatc ggcgagaacg atctccaact cgttgaggtg gtccgctgtc gtgccataga
     6781 ccggcgtgtg cgagccgctc gaattgttcg cgatcatgcc gcccagcgtc gcccgtgagc
     6841 ttgttgccac gtcgggtccg aagcagaagc cgtgcgggtg caggaacttg ttcaggttat
     6901 cgagcacgac ccccggtccg acccgcaccg tgcgccgctc caaatccagg tcggtgatcg
     6961 cgcggttgtg gcgggagaaa tcaatgacca ggccgtcgcc gatggccccg ccggccagcc
     7021 ccgtgcctgc gccgcgggga atgaccgtaa tggacgcctg gaccgcagcg tcaagcaggg
     7081 cgctgacttg gggcgcgtta gcgggaaac
//
LOCUS       DATCGS010000168         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_411688, whole genome shotgun sequence.
ACCESSION   DATCGS010000168 DATCGS010000000
VERSION     DATCGS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411688"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2561
                     /locus_tag="VIL39_03395"
     CDS             <1..2561
                     /locus_tag="VIL39_03395"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480500.1"
                     /translation="AGEKKIVVLQPRRVAARTVAARVAWERRCQLGAEVGYQIRFDDQ
                     TSLGTRISYVTEGILLRWLQADRTLADVGVVIFDEFHERNLLSDVALALVKRLQRTER
                     PDLKMAVMSATLEAEPVAEYQAGQGGTGETPVLHSEGQSFPVEVRYLSQHDERPITEQ
                     AADVVEQIVNSGEPGDILVFMPGMGEINATISAARAIRVNERLTFIPLHGDLPPEQQD
                     LAFAPNSLRKVVVATNVAETSVTIDGIRHVVDGGLARVARYDAERGIGTLFMEPISRA
                     SAEQRKGRAGRTAPGTCYRLWTESGQLNRPERNTPEIQRSDLAEVVLLLHSLGIKRAA
                     EFDWLDKPDPQAVERAEQLLITLGALRESRITTDALRTGREAESPSANSQSTIEADLT
                     PIGRQMLKLPMHPRYSRMLVEAAKCRCVPAAALCAALVSGRDLLMRLGRDDKHIQEAR
                     ELFEASQESDFYTLMRAYQFARKNNFSVETCRRYGIHAQTARQVEQTFEQIMQIAKQQ
                     GLLKSEETGPTAEPNVITHHASRITPPPDPLPRCIMTGFIDQLCLRRDQGTLECDLTE
                     GRHGTLMRESVVQSAPLFVAATIREVTGRGSENLTLLGLASAVKREWVEETFPEQVTA
                     KVEHLYDRTHKRVAAVKLVRFRDLVIHHEHQREVDPKASGRCLAEAYRKGYFELPLLN
                     HELKQFISRVNLVVAAMPELEFPPFDEAAITGCLARAFEGLTLAKEAQATPLRDAFHQ
                     ALAREQIGWLDELAPTSILWPDGRKVKLQYPEQPRDEEGQPNSPELQVKLHECFALKA
                     HPHLCEGRLPVKLWLCAPEGKRLEATFNWTAFRANSYPKLKPALQKKHPGVPWP"
     gene            complement(2789..2941)
                     /locus_tag="VIL39_03400"
     CDS             complement(2789..2941)
                     /locus_tag="VIL39_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY5480501.1"
                     /translation="MLYTIAVVLLILWLLGLVSGYAIGGLIHLLLVVAIVMVLLNFIS
                     GRRRSV"
BASE COUNT          598 a          947 c          985 g          512 t
ORIGIN      
        1 tcgccgggga aaagaagatc gtcgtcctgc aaccccggcg ggtggcggcg cggacggtgg
       61 ccgcccgggt cgcgtgggaa cgccggtgcc agctcggcgc ggaggtcggc taccagattc
      121 gttttgatga ccagacaagc cttggcaccc gcatcagtta tgtgaccgaa gggattctgc
      181 tgcgctggct acaggccgat cgaacgctgg cagacgtggg cgtggtcatc ttcgatgagt
      241 tccacgagcg caacctgctg agcgacgtgg ctctcgcgct ggtgaagcga ctgcagcgca
      301 cggagcgtcc cgatttgaag atggccgtta tgtcggcgac gctcgaggcg gagccggtgg
      361 cggagtatca ggcggggcag ggggggacag gcgagacgcc ggtcctgcat tccgaggggc
      421 agagctttcc ggtggaggtg cgctatttga gccagcacga cgagcgacca attactgaac
      481 aggccgccga cgtggtcgaa cagatcgtga actcaggcga gcctggcgac atcctggttt
      541 tcatgcccgg catgggcgag atcaacgcta ccatcagcgc cgcccgcgcc atccgggtga
      601 acgagcggct cacctttatc cccctgcacg gcgacttgcc gcctgagcag caggacctcg
      661 ctttcgcgcc gaattctctg cgcaaggtgg tcgttgccac gaatgtcgcg gagacctcgg
      721 tcaccattga tggcattcgc cacgtggtgg atggcgggct ggcacgggtc gcgcgctacg
      781 atgcggagcg cggcattggg acgctcttca tggagcccat cagccgggcc tccgccgagc
      841 agcgcaaggg ccgcgccgga cgcaccgcgc cgggcacctg ctaccggctt tggactgaaa
      901 gtggccagtt gaaccggccc gagcgcaaca cgcccgagat tcagcgcagc gacctggccg
      961 aggtggtgtt gctgctgcac tccctcggca tcaagcgcgc cgcggagttt gattggctgg
     1021 acaagccgga cccgcaggcg gtcgagcggg cggagcagtt gctgatcacg ctgggggcgc
     1081 tgcgggagtc aagaatcacg actgacgctt tacgcaccgg ccgggaagcc gaatcgccaa
     1141 gcgccaatag tcaatcgaca atcgaagccg atctgacgcc cattggccgg cagatgctca
     1201 agctgccgat gcacccccgc tactcgcgca tgctggtaga ggcggctaaa tgccgttgcg
     1261 tgccggcggc ggcgttgtgc gctgcgctgg tcagcgggcg cgatttgttg atgcgccttg
     1321 gccgcgacga caagcatatc caggaggcgc gcgagctatt cgaggcgagc caggaatcgg
     1381 acttctacac gctcatgcgc gcctatcagt tcgcccggaa gaacaacttc agcgtcgaga
     1441 cctgccgccg ttatggcatc cacgcgcaga cggcgcggca ggtcgagcag acgttcgagc
     1501 agatcatgca gatcgcgaag cagcagggcc tcctgaaaag cgaggaaacc ggcccaacgg
     1561 ccgagcccaa cgtcatcacg catcacgcat cacgcatcac gcctcccccc gatcccttgc
     1621 cccgctgcat catgactggc tttattgacc aactctgcct ccggcgcgac caggggacac
     1681 tggagtgcga cttgaccgaa gggcggcacg gcacgctgat gcgcgagagc gtcgtgcaaa
     1741 gcgctcccct gttcgtggcg gcgacgattc gcgaggtgac cgggcgcggt tcggaaaacc
     1801 tgacgctgct cgggctagcc agcgcggtga aacgcgagtg ggttgaagag acgttcccgg
     1861 agcaggtcac cgcgaaggtg gagcacctct acgaccgcac ccacaagcgc gttgccgcgg
     1921 tgaagctggt ccgcttccgg gacctagtca ttcatcacga gcaccagcgc gaggtggacc
     1981 cgaaagcttc cggccgatgc ctggcggagg cgtatcggaa aggctatttc gagctgccgt
     2041 tgctcaacca cgaactgaag cagttcatca gccgggtgaa cctggtggtg gcggcgatgc
     2101 cggagctgga attcccacct ttcgatgagg cggcgattac cggttgcctg gcgcgggcgt
     2161 tcgaggggtt gaccctggcc aaagaggcgc aggccacacc actgcgcgac gcctttcatc
     2221 aagcgctggc cagggaacag atcggctggc tcgacgaact cgcgcccacg agcatccttt
     2281 ggccggatgg ccggaaggtg aagctccaat acccggagca accgcgtgat gaggagggtc
     2341 agccgaattc ccctgagtta caggtgaaac tccacgagtg cttcgcgctg aaggcgcatc
     2401 cgcacctctg cgaaggccgc ttgccggtga agctctggct ctgcgcgccc gagggcaaac
     2461 gactggaagc caccttcaat tggaccgcgt tcagggcgaa cagctatccg aagctcaaac
     2521 ccgcgctcca gaagaagcac cccggcgtgc cgtggccgtg acttggcagg gacgcatgcc
     2581 acggcgtccc ggtggtgacc gtctccggct ggatcgcgca gccggtggcg ctatccagga
     2641 accgcacctg gacggtttgc gcctccagtg ccgctggaag catggctgct tgaaacgtga
     2701 agatcacgac ccaccgagcg aggctaactt tccccactct gccagcaaaa aagcaccgca
     2761 aaccccagtc aatgcctgcc ccagtttctt aaaccgagcg tctgcgcccg gaaatgaagt
     2821 tgagcagaac catgacaatg gcaactacca gcaggaggtg aattaaccct cctatcgcat
     2881 aaccgctcac caggccgagc agccacagaa tgagcaggac taccgcaatt gtgtataaca
     2941 tatgtttttc ctttccgttt gttttggagt cgctatgggc cggcgcgtcc gcgccagccc
     3001 acagagattc agtattcaat tcttgctcag agtcgtagcg at
//
LOCUS       DATCGS010000169         3498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_164949, whole genome shotgun sequence.
ACCESSION   DATCGS010000169 DATCGS010000000
VERSION     DATCGS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164949"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VIL39_03405"
     CDS             <1..436
                     /locus_tag="VIL39_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480502.1"
                     /translation="VILGLFWQTNPAALGRPERLGLGFGAALIAPMLNLILLQIPNAA
                     ALAFPAWFQSAKGGGQGIEATGQRLIFMLGQVLVLLVVLLPAAALFAGVFFLARWLLA
                     TFVAIPLASIAAALVLAVEAALGVMLLGWLFDRFDVAAEQTS"
     gene            583..1509
                     /gene="htpX"
                     /locus_tag="VIL39_03410"
     CDS             583..1509
                     /gene="htpX"
                     /locus_tag="VIL39_03410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY5480503.1"
                     /translation="MNIVQVSKRIFLLVVVNILVVTTITLVLGLFHVGRYFPQGGLQG
                     LAVFCLVWGFGGAFISLGLSRLMAKWMMGVQVIPPNTTDPTLRELVETVHALARTAGL
                     PKLPEVGIYKSDEVNAFATGPTKSRALVAVSTGLLQRMGSQEVAGVLGHEITHIANGD
                     MVTMTLIQGIINSFVLFLSHVLAFVISQAMRSRDDRDSGGGGWLQFILVMVFQIIFSV
                     LGSLVVCWFSRWREFRADAGGARLAGRENMINALRALGRLHDPEVAAAEASRTQAFRS
                     LKISGQAGGLMALLATHPPIERRIARLEQGAF"
     gene            complement(1594..1668)
                     /locus_tag="VIL39_03415"
     tRNA            complement(1594..1668)
                     /locus_tag="VIL39_03415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1634..1636),aa:Asn,seq:gtt)
     gene            complement(1768..>3498)
                     /locus_tag="VIL39_03420"
     CDS             complement(1768..>3498)
                     /locus_tag="VIL39_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5480504.1"
                     /translation="VIDTRTDQIVETVCARQNPADLFGAQPNALAFDKSGGKLYVCNG
                     TQNAVAVFDFKPGQSILRGLIPVGWFPGGIAYDRARKALCVANIKGIGSTKHLKPGEP
                     VKYNSHLYFGTLSLVPVPDERELPRLTQTALLNLRYSLLQEAKLPARPDQPARPVPER
                     AGEPSFFKHVVYIIQENRTYDQVLGDLPQGNGDPSLCVFGERITPNHHKVAREFVLLD
                     NTYCSGILSADGHQWADSAFANDYMEKSFAGFPRSYPFGGTEDSVDALAYSSAGFIWD
                     NVIAHGKTLRDYGEFAITEAHWRDKSKTHAPGFLDCYRDFIDQAGEIELGCRPSIESL
                     RPYLVTNTVGWALHVPDVFRAAQFIRELKQFEQTGNFPNLCIISLPNDHTSGTKSGCP
                     TPAAMAADNDLAFGQIVEAISRSRFWKETCIFAIEDDPQAGWDHVSGYRTTSYVASPY
                     TRRAAVVSTQYNQTSLLRTMELMLGLPPMNQMDATATPMTACFTDTPDLTPFSALTNN
                     IPLDQMNPAPKKITDSLLRKHAYASARLPLDEADKCPEDTLNRILWHALKGSQTPYPA
                     WAVSAVCDDD"
BASE COUNT          597 a         1085 c         1114 g          702 t
ORIGIN      
        1 tgtgatcctc gggttgttct ggcaaacaaa tccagccgct ctcgggcgac cggagcggct
       61 gggcctgggt ttcggcgccg ccctcatcgc gcccatgctc aacctgatcc tcctccagat
      121 tcccaacgct gccgcgctgg cattccccgc gtggttccag tcggccaagg gcggcgggca
      181 aggcatcgag gccaccggcc agcggctcat cttcatgctc ggccaggtgc tcgtcctcct
      241 tgtggtcctg ctcccggccg cggccctgtt tgccggcgta ttcttcctgg caagatggct
      301 gcttgcaaca ttcgttgcca tccccctcgc ctcgattgct gccgctctcg tcttggccgt
      361 cgaagcagcg ctgggcgtca tgctgctcgg ctggctgttc gaccggtttg acgtggcggc
      421 cgagcagacc agttgaacat ctctgcctgt tgcagaacgt ccgcaacctg ctatgctttc
      481 cgtcgcaggg tgaccgccca acgtgtaaga ctcaagggcg gcaacctccc gggagtttcc
      541 ggtaacacaa acaaattact ttagataaga cgactgatag ttatgaacat agtgcaggtt
      601 tcaaagcgaa tctttttgct cgtagtggtg aacatcctgg tggtgaccac catcaccctg
      661 gtgctgggcc tctttcacgt gggacgctac ttccctcagg gcggccttca ggggctggcc
      721 gtattctgcc tcgtctgggg ctttggcggc gccttcattt ccctcggcct ctcacgcctc
      781 atggccaagt ggatgatggg tgtccaggtc atccccccga acaccaccga ccctaccctg
      841 cgcgaactgg tggagaccgt ccacgctctc gcccgcacag ccggtctccc caagctgccc
      901 gaagtcggca tctacaagtc tgacgaagtc aacgccttcg ctaccggccc caccaaatcc
      961 cgggccctgg tggccgtctc gaccggcctg ctccagcgca tgggcagcca ggaggtcgcc
     1021 ggtgtcctcg gccacgaaat cacccacatt gccaacggag acatggttac catgaccctc
     1081 atccagggta tcatcaactc cttcgtcctg ttcctctccc acgtcctggc tttcgtcata
     1141 agccaggcca tgcgctcccg cgacgatcgc gacagcggcg gcggcggctg gctccagttc
     1201 atcctggtga tggtgtttca gatcatcttc tccgtcctgg gctcgctggt ggtgtgctgg
     1261 ttctcccgct ggcgcgagtt ccgggccgat gcgggcggcg cccggctcgc cggacgggaa
     1321 aacatgatta acgccctgcg cgccctaggc cggctccacg accccgaagt cgccgccgcc
     1381 gaggcctccc ggacccaggc cttccggtcc ttgaagatct ccggccaggc cggcgggctc
     1441 atggccctgc tcgccaccca tccgcccatc gagcgacgca tcgcccgcct cgaacaaggg
     1501 gccttctgat ctttgctgtc aattccgctg acagtgaccc gctaaacggc ctgattggaa
     1561 gcacaagtcc ttgtcccgca ggcacaaacg aattggctcc agaggcaggg ctcgaacctg
     1621 caacccgccg gttaacagcc ggctgctcta ccattgagct actctggatg ccagagagag
     1681 gcgcaatcta caaagcggca tgcgccgcgt caaacggatt ctcacccaac attctcacca
     1741 ccggtaagcg ggaaggccgg ttccggctca gtcgtcatcg cagactgcgc tgacggccca
     1801 cgcgggatag ggggtttgcg agcctttgag cgcgtgccag aggatgcggt tgagcgtgtc
     1861 ttcgggacat ttatccgctt cgtcgagcgg caggcgcgcg gaagcgtaag cgtgcttgcg
     1921 caggagcgaa tcggtaatct tcttcggggc gggattcatc tggtccaggg ggatgttgtt
     1981 cgtcagcgcg ctgaatgggg tgagatcggg cgtgtcagtg aagcacgcgg tcatgggggt
     2041 ggcggtggcg tccatctggt tcatcggcgg caggccgagc atcaactcca tcgtgcgcag
     2101 gaggctggtc tggttgtatt gagtgctgac cacggcggcg cgccgggtat aggggctggc
     2161 gacgtagctg gtggtccgat agccgctgac gtggtcccaa ccggcctgcg gatcgtcctc
     2221 gatggcgaag atgcaggttt ccttccagaa ccggctccgg ctgatggctt caacgatttg
     2281 gccgaatgcc aggtcgttgt cggccgccat cgcggcgggg gtggggcagc cgctcttggt
     2341 gccactggtg tggtcgttcg gcaggctgat gatgcagagg ttggggaaat tgccggtctg
     2401 ttcgaactgc ttcaactccc ggatgaactg cgcggcgcga aacacatcgg gaacatgcag
     2461 agcccagcct acggtattgg tgacgaggta gggccgcaac gattcgatgg aagggcggca
     2521 gccgagctcg atctcgccgg cctggtctat gaagtcgcgg tagcaatcga gaaacccggg
     2581 cgcgtgcgtt ttcgatttgt cccgccaatg cgcctcggtg atggcgaact cgccgtagtc
     2641 acgcagcgtc ttgccatgcg cgatgacgtt gtcccagatg aaaccggcgg acgaatacgc
     2701 cagcgcgtcc accgagtctt cagtcccgcc gaagggataa ctgcggggga aaccggcgaa
     2761 tgacttctcc atgtaatcat tggcgaaggc actgtccgcc cattgatgcc catcggcgct
     2821 gaggatgccg gagcagtagg tgttatcgag caggacgaac tcacgcgcca ccttgtggtg
     2881 gttgggggtg atccgctcgc cgaagacgca gagtgacggg tcgccgttgc cctgcgggag
     2941 gtcgccgagc acctggtcgt aggtgcggtt ctcctgaatg atatagacga catgcttgaa
     3001 gaagctgggc tcgcccgcgc gctcggggac gggccgagcc ggctggtccg gacgcgctgg
     3061 cagtttggcc tcttgcagca aactatagcg aaggttaagg agggcggtct gggtcaggcg
     3121 cggcaattcc cgctcgtccg gcacgggcac cagcgacaag gtgccgaaat acaggtgtga
     3181 gttgtatttg acgggctcgc ccggcttgag gtgtttggtc gagccgatgc ctttgatgtt
     3241 ggcgacacag agcgccttac gggctcggtc gtaagcgatg ccgccgggga accagccgac
     3301 agggatcagg ccgcgcagga tggactggcc gggtttgaag tcgaagaccg ccaccgcatt
     3361 ctgggtgccg ttgcagacat agagcttgcc gccggacttg tcgaacgcga gcgcgttggg
     3421 ctgcgccccg aacaaatccg cagggttctg ccgcgcacag acagtctcga cgatctggtc
     3481 tgtgcgagtg tcaatcac
//
LOCUS       DATCGS010000170         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_210838, whole genome shotgun sequence.
ACCESSION   DATCGS010000170 DATCGS010000000
VERSION     DATCGS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210838"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2263
                     /locus_tag="VIL39_03425"
     CDS             <1..2263
                     /locus_tag="VIL39_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5480505.1"
                     /translation="DDALFTRFEKTLVAKCVSGHEQEYITMRLADQAARRAKFGNSAC
                     LQEPNLKNGCGGLRDYQNLLWMAFFKYRTHSLKELQTHALIGEGEHKQLEAAHDFLLR
                     VRTEMHYYANREMDVLGKNLQPAVAYNLGYRERSPSKRIEEFMRDLYTHSRNIYLITR
                     TLEQRLALLPQARRLSGFSLPVSLSGLFPGGRKRITEPVDGFRFIDGEIHAASSRVFR
                     DQPRRLMRVFLYAQQRGLRLHPDLAQLMRNQIGLVDRAFLSDTHVRESFLAILNQRGS
                     VAPILRAMHEVDLLGKYLPEFGKLTCLVQHEFYHQYTADEHTLMCLEQLDRIWEAKAP
                     PYLIYNVLFQKLERPFVLYLALLLHDVGKADSHGNHSRASCDLALRVAKRLHLDAADT
                     QTLLLVIEHHLLMTRVSQRRDLDDPAVIRRFAKQVQTPETLALLTLHTFVDSQATSDN
                     LWNGFKDLLLSSLYHKTLQLMIGGPEFARAGEKHRELLMQEVRRLAPEPPSEEELQAH
                     FANLPARYFQIHPAKEILGDLVLAHRFMRLQIAEGEDALAPVVCWHNEPDRGYNGVKV
                     CTWDRAGLFRKIAGSLSAAGLNILSAQIFTRTDDIALDTFFVADAITGNLAGADQRDR
                     FESGLLKALTGEEVDFPALIRRQKITRPLYQAYTGEKIATQIRLDNEDSESRTLIEIE
                     TEDRIGLLYTISQTLTELALDISAAKICTERGAALDSFYVREAHSGKVLAPERHRSIE
                     RQLRHAIHALDAR"
     gene            complement(2559..2747)
                     /locus_tag="VIL39_03430"
                     /pseudo
     CDS             complement(2559..2747)
                     /locus_tag="VIL39_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2744..>3424)
                     /locus_tag="VIL39_03435"
     CDS             complement(2744..>3424)
                     /locus_tag="VIL39_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HEY5480506.1"
                     /translation="QMVQAARSGGKNIAEGSRAAATTSQTELRLVNVARASLEELLLD
                     YEDYLRQRKLRAWEKDDPEALAVRAVGKQLDQTDQTDRTDPVAHYQAYARWLEHADPA
                     VVANTLICLIHQTNYLLDQQISALERDFVKEGGYSEQLAAARIAERRRQDRTDETDRA
                     DRTEAAAKAPNCPRCGKPMALRTARKGPRAGSQFWGCSGYPECKGTRPLEAAVGLDRP
                     DGSKQGKP"
BASE COUNT          606 a         1259 c          930 g          629 t
ORIGIN      
        1 cgacgacgct ttgttcacgc gcttcgagaa gaccctggtc gccaaatgcg tctccggcca
       61 tgagcaggaa tacatcacga tgcgcctggc cgatcaggcc gcccggcgtg ccaagttcgg
      121 caactcggcc tgcctgcagg aacccaacct caagaacggc tgcggcggcc tgcgcgacta
      181 ccagaacctg ctctggatgg cgttcttcaa ataccgcacc cactcgctta aggagctgca
      241 gacgcatgcg ctcatcggcg agggcgagca taagcaactg gaagccgccc atgacttcct
      301 gctccgcgtc cgcacggaga tgcactacta cgccaaccgg gagatggacg tgctcggcaa
      361 gaatctgcaa cccgccgtcg cctataacct cggctaccgt gaacgctccc ccagcaagcg
      421 catcgaggag ttcatgcgcg acctctacac ccactcgcgc aacatctatc tcatcacgcg
      481 caccctcgag caacggctgg ccctcctgcc gcaggcacgc cggctttcgg gcttttcgct
      541 gcccgtcagc ctctccggac tgttccccgg cggacgcaag cgcatcactg agcccgtgga
      601 cggcttcagg ttcattgacg gtgagatcca cgccgcctcc agccgcgtct tccgcgacca
      661 gccgcgccgg ctcatgcgcg tgttcctgta tgcgcagcag cgcgggctgc gattgcatcc
      721 tgacctggcc cagctcatgc gcaaccagat cggcctggtg gatcgcgcgt tcctctcgga
      781 cacgcacgtc cgggaaagct tcctggccat cctcaaccag cgtggcagcg tcgcgcccat
      841 cctgcgcgcc atgcacgagg ttgacctgct cggcaagtat ctcccggagt tcggcaagct
      901 cacctgcctg gtccaacatg agttctacca ccaatacacc gccgacgagc acacgctgat
      961 gtgcctcgaa caattggacc gcatctggga ggccaaggcg ccgccctact tgatctataa
     1021 cgtcctcttc cagaagctcg agcggccctt cgtgctctac ctggccttgc tcctccacga
     1081 tgtgggtaaa gctgacagcc acggcaacca ctccagggca agttgcgacc tggccctgcg
     1141 cgtcgctaaa cgcctccacc tcgacgccgc cgacacccag accctgctgc tcgtcatcga
     1201 gcaccacctg ctgatgaccc gcgtctcgca gcgccgcgac ctcgatgacc cggcggtaat
     1261 ccggcggttt gccaagcagg tgcagacccc cgaaacgctc gccctgctca cgctgcacac
     1321 cttcgtggac tcccaggcca ccagcgacaa tctgtggaac ggcttcaaag acctcctgct
     1381 gagttcgctg taccacaaaa ccctgcaact gatgatcggc ggccccgagt tcgcccgcgc
     1441 gggggaaaag caccgcgaac tgctgatgca ggaagtccgc cgccttgcgc ccgagccccc
     1501 gagcgaggag gaactccagg cccacttcgc caacctgccc gcgcgctact tccagatcca
     1561 tcccgccaag gaaatcctgg gcgacctcgt cctcgcccac cgcttcatgc gcctccagat
     1621 cgccgagggg gaagacgcgc tggcccccgt cgtctgctgg cacaacgagc ccgaccgcgg
     1681 ctacaacggc gtcaaggtct gcacctggga ccgcgccggc ctcttccgca agatcgccgg
     1741 ctccctcagc gccgccggcc tgaacatcct cagcgcccaa atcttcaccc gtaccgacga
     1801 catcgcgctc gacaccttct tcgtcgccga cgccattacc ggcaacctgg ccggcgcgga
     1861 ccagcgcgac cgctttgaaa gcggcctcct caaagccctc accggcgagg aagttgactt
     1921 cccagccttg atcaggcggc aaaagatcac ccgcccgctc taccaggcct ataccggcga
     1981 aaagatcgcc acccaaatcc gcctcgacaa cgaggactcc gagagccgca ccctcatcga
     2041 aatcgagacc gaagaccgta tcggcctgct ctacactatc tcgcagacgc tcaccgagct
     2101 ggccctggac atttccgcgg cgaagatctg caccgaacgc ggcgcagccc tggacagctt
     2161 ctacgtccgc gaagcgcaca gcggcaaggt cctcgcgccc gaacggcacc gatccatcga
     2221 acgccagctc cgccacgcca ttcacgccct ggatgcgcgg taagcccgcg cccgcccagc
     2281 ctgcccttgt ccccggcttg tccctcgcat gtaactgact tgtgcgttgc ttgggccccc
     2341 agtcacgaat cacgaatcac gcatcactcc tggctttgtg gtggccttga ggtggctttg
     2401 ggcggctgtg cccggccgtt caaggttcaa ggttcgaggt tcgaggttca aagttcagta
     2461 ttttccataa gcataccgaa tacaactccc ctcccgcgcc tcccttgggg tggtctggtg
     2521 gcaccctgga catatccgct tcggtccgta gcaggttaga gggcgcggct aatctgctcc
     2581 ttggaatagc ccgcccgggt caggtagctg cccaggagat cctcttcgat attgctattg
     2641 cccggacggt cggcgcggtc accgaggtaa cggtagtgga gttcgtcgcg gaacagcgcg
     2701 atcacacggt tctgcgtttc gcgttcaggt ttgccgatgg tgctcatggt tttccctgct
     2761 ttgatccgtc aggccggtct aatccgaccg ctgcttccag tggccgcgtg cctttgcatt
     2821 ccggataacc tgaacagccc caaaactgcg atcccgcacg aggccctttg cgcgccgtgc
     2881 gcagggccat gggcttgccg caacgcgggc aattgggggc tttagccgcc gcctcagtcc
     2941 ggtccgcccg atcggtctca tctgtccgat cttgcctcct ccgctccgca atccgcgccg
     3001 ccgccaattg ctcgctgtaa ccaccctcct tcacaaaatc ccgctccagc gcgctgatct
     3061 gctgatcgag caggtagttc gtctggtgaa tcaggcaaat cagcgtgttg gccaccaccg
     3121 ccgggtcagc gtgctccagc cagcgcgcgt aggcttggta gtgggccacg ggatccgtcc
     3181 ggtctgtctg atccgtctga tccagttgct ttcccaccgc ccgcacggcc agcgcttcgg
     3241 ggtcgtcctt ctcccacgcc cgcaacttcc gctgccgcag ataatcctca taatccagca
     3301 gcaactcctc caggctcgcc cgcgcgacat tcaccagtcg tagctcggtc tggctcgtcg
     3361 tcgcggcggc gcggctgcct tccgcgatgt tctttccgcc gctgcgcgcc gcctgcacca
     3421 tctg
//
LOCUS       DATCGS010000171         3054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_511471, whole genome shotgun sequence.
ACCESSION   DATCGS010000171 DATCGS010000000
VERSION     DATCGS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511471"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VIL39_03440"
     CDS             complement(<1..358)
                     /locus_tag="VIL39_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY5480507.1"
                     /translation="MMEKFLATTWNRLVASGKRDTETGSRLDLGFQVIDGEVAHARAY
                     LPDSKRCEHVAILGKTGQGKSFFLRHLSTQDIHRRNGFVFFDLHGDTMPFLLRVIAAA
                     ERRTGTDLSDRLVVIEP"
     gene            complement(368..772)
                     /locus_tag="VIL39_03445"
     CDS             complement(368..772)
                     /locus_tag="VIL39_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480508.1"
                     /translation="MNHSDLSATGFQTCTTLDGRLERVGVGRRNGHLTVLLSPDWADG
                     GPRRAQSRLWSDDPRYYVNLNGVERRTWRRILSGASISAIAAEEGVSRAAIYARIQGN
                     SEGHGGMIAKNYWCLLWWRLRKSLLTPHRQDQ"
     gene            957..2099
                     /locus_tag="VIL39_03450"
     CDS             957..2099
                     /locus_tag="VIL39_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5480509.1"
                     /translation="MPSSINPEAAGHAIAELRQSLGLTQKEVAASISTHYSDDRAYRR
                     VERGERLPDRDKVIAILNSGLSIGQTETINRVLGLAGYAALTESEVWSLKPASVMGPV
                     STPLVPQRASAEFWHGPSGRKRLVAVVVSSLALSGAIAFTGPNGIMVMLAAVLYSSLY
                     TVSVLLESAFGPNSGKTVPAGAGVFCLMLLTSVLALAVDGWLVDAGNAAALPVSLAVF
                     VLSAGLQWLLVKSALPDSAVVPSRFASHTAQAAHLKNTLYFLLIVVLFWLPPIHCIAV
                     LRREIRAGNAALAKAMLERTVIIGRDVVCLNSTWLWGLFLFLALLSLPMGAWLTNNLN
                     PHPKTNTYTNLLYLRAFLYFLLILACLIWFSSSIGSLPLQHPRFES"
     gene            2193..2567
                     /locus_tag="VIL39_03455"
     CDS             2193..2567
                     /locus_tag="VIL39_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480510.1"
                     /translation="MQNETQGNEQQLNLSVDLGGGRQVDVEIGTPDQLWVSAGPGIGL
                     GDVWMKGDGRLIDLRVRIRRTPGGVEIVVDRGDTGDPWWPAGGAPRLAVAIDPAARTA
                     LAVASAGTDGQFQEVMLGKTAI"
     gene            complement(2680..>3054)
                     /locus_tag="VIL39_03460"
     CDS             complement(2680..>3054)
                     /locus_tag="VIL39_03460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY5480511.1"
                     /translation="DANTGRHLRAAISENPGFRYPRSVLHVPSESNTVHHTQKPLALF
                     EYLIKTYTNPGELVLDSCMGSGTTAIACLNTGRNFIGFELDPECFQAAQHRLRTRQCS
                     LQKSGNGETPPSEASASATVSC"
BASE COUNT          567 a          865 c          969 g          653 t
ORIGIN      
        1 cgggttcgat gacaacaagc cggtcgctga gatcggttcc ggtccgccgc tccgcggcgg
       61 ctataacccg gagcagaaaa ggcatggtgt cgccgtgaag gtcaaagaaa acgaagccgt
      121 tgcggcggtg gatatcctgc gtactgaggt ggcgcaggaa gaacgacttt ccctggccgg
      181 ttttgccgag aatagccacg tgctcgcagc gcttggaatc cggcaggtag gcgcgggcat
      241 gtgccacttc gccatcaatg acctggaagc ccaagtcaag acgcgatccg gtttccgtgt
      301 cgcgtttacc gctggcgacc aggcggttcc aagttgtggc gagaaacttt tccatcatgg
      361 tggttcgtta ttgatcttgg cgatgtggtg taaggaggct cttacggagg cgccaccaca
      421 acaggcacca gtagttcttg gcgatcatgc cgccgtggcc ttcgctgttg ccctggatgc
      481 gggcgtagat ggcggcgcgc gagacgccct cctcggcggc aatggcgctg atactggccc
      541 cggacagaat ccgtcgccag gtgcggcgtt cgactccgtt caggttgacg tagtacctgg
      601 ggtcgtcact ccaaagacgg gactgggcgc gccgtggtcc gccatccgcc cagtccggcg
      661 acagcagcac cgtcaggtgg ccgttgcgcc ggccgacgcc gacacgttcg agccttccgt
      721 ccagagttgt gcacgtttgg aagccggtag cggaaagatc cgaatgattc acggcccact
      781 attggccgga gagcggcgaa tttgggagga ccacaatcgg gaggaccatg accggaccga
      841 cacaggacca gtaatgaacc gataagtcct ttgactaaag gcatccccac tcttccttgc
      901 cggtccgagg ctttctggct atacttcggc tagactctcc ctttcagagc aagactgtgc
      961 cctcatccat aaatcccgag gcggctggcc atgcgatagc agaattgcgc cagtccctgg
     1021 gtcttaccca gaaagaagtg gccgccagca tcagtaccca ttacagtgac gaccgggcct
     1081 accgtcgagt ggaacgcggt gagcggctgc cggaccggga caaggtgatt gccattctca
     1141 actcggggct ctccataggt caaaccgaaa ctatcaaccg ggttctcggt ctggcagggt
     1201 acgccgctct aactgaatct gaggtgtgga gtctcaaacc tgcatccgta atgggtccgg
     1261 tatccacgcc gctagttcca caaagggcca gtgcggagtt ttggcacggt ccgagcggcc
     1321 ggaaacgcct ggtcgccgtt gttgtctcct cgctggccct gagtggcgcg atcgctttta
     1381 ccggtccaaa cggaatcatg gtgatgctgg ccgccgtgct ctacagcagc ctgtacactg
     1441 tctcggtgct gctggagagt gcgtttgggc cgaattccgg aaaaacagtt cccgcgggcg
     1501 ccggggtgtt ttgcttgatg ctgctgactt cggttctggc attggcggtt gacggatggc
     1561 tggtggacgc cggcaatgca gcagcgctgc cggtgtcgct ggcggtattc gtactgtcgg
     1621 ctggattgca atggctgttg gtgaaatctg cgctgccgga ctccgccgtg gtcccgtccc
     1681 gctttgcatc ccataccgcc caggcggccc atctgaagaa cactttgtac ttcctgttga
     1741 tcgtcgtgct tttctggttg ccgcccattc attgcatcgc ggtgcttcgc cgtgagatcc
     1801 gggccgggaa cgccgccctt gcaaaagcga tgctggagcg gacagtgatc atcggccgtg
     1861 atgtcgtctg cttgaactcc acgtggctct ggggactgtt cctgttcctg gcgctgctgt
     1921 cccttcccat gggcgcctgg ctgacaaaca acctcaatcc tcaccccaag accaacacct
     1981 atacgaactt gctttacttg cgcgcgtttc tttacttcct cctcatcctt gcctgtttga
     2041 tctggttctc gtcctcgatt ggatccctgc cgctccaaca cccgcggttc gaatcataga
     2101 gccacctcgc attcagatct caagcctgcc ttcgggctgg agcacaatct ccgaatcctc
     2161 ggacttgcgc atcgggccga aggagggcag aaatgcagaa cgaaacgcag ggaaacgagc
     2221 agcaactcaa tctgtcagtg gatttgggcg gcggcagaca ggtggacgtg gaaatcggta
     2281 cacccgatca gctctgggtc tccgctggac ctggtatcgg attgggtgac gtctggatga
     2341 agggcgatgg ccggttaatt gacctgagag tgcgcatccg gcggacccca ggcggcgtcg
     2401 aaatcgtcgt ggaccggggc gataccggag atccatggtg gcccgcaggt ggcgcgccga
     2461 ggctggcggt cgcaattgat cctgcggcaa gaacagcgct cgccgtcgct agcgcgggca
     2521 cggacgggca gtttcaggag gtcatgctcg gtaagacggc gatctgattc gtttgcggca
     2581 acgctgcgcg ccggagcagc atgcctgagg gctggttcca gattggagcc ggcctttttc
     2641 atttgtcggg agatgcatca gcgggatcga aattgaggat caacaactca ctgtagcgct
     2701 tgcggacgct tcgctcggcg gtgtctcgcc gttgccggac ttttggaggg aacattgacg
     2761 ggtccgtagg cgatgctggg cagcctggaa acattcggga tcgagttcaa agccgatgaa
     2821 gttgcggccg gtattgaggc aagcgatggc ggtagtgccc gatcccatac aactgtcaag
     2881 caccaactct ccgggattgg tgtaggtttt gatcaggtat tcaaatagcg ccagtggctt
     2941 ttgggtgtgg tggacggtgt tggactcgga cggtacatgc agcacgctgc ggggatagcg
     3001 gaagccggga ttctcggaga tggcagcgcg gaggtgcctg cccgtgttgg catc
//
LOCUS       DATCGS010000172         3694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_461956, whole genome shotgun sequence.
ACCESSION   DATCGS010000172 DATCGS010000000
VERSION     DATCGS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461956"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..997)
                     /gene="gluQRS"
                     /locus_tag="VIL39_03465"
     CDS             complement(95..997)
                     /gene="gluQRS"
                     /locus_tag="VIL39_03465"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY5480512.1"
                     /translation="MVAAETLRQAIHASRIMYRGRLAPSPTGYLHLGHARTFWIAQQR
                     ARASGGVLVLRNEDLDAKRFKLEFVSAMLEDLKWFGFEWQEGPDCGGPFGPYNQSERR
                     EFYLAAMRKLLAAGFIYPCTCSRSDVRRALSAPHQGEDEPVYPGTCREKFKPDGEACA
                     FDPNPTSGSPQKANWRFRVPDGETITFTDGHCGPQQFVAGKDFGDFVVWRHDGVPAYQ
                     LSVVVDDAAMRITEVVRGADLLPSTARQLLVYRALGVPAPQFYHCPLMTDEKGVRLAK
                     RHDALSLRRLRAEGNTPEQIRAQW"
     gene            complement(1130..2068)
                     /locus_tag="VIL39_03470"
     CDS             complement(1130..2068)
                     /locus_tag="VIL39_03470"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY5480513.1"
                     /translation="MSSRTEVLTIEHSLPSVRLDMYLRGKFPAVSRGAIQRLIVQGYI
                     RVNGRTVKPTHAPRAGEQVEVHWPEARSAEAQAEKMPLNILYEDEALLVLNKPPGLVV
                     HPGAGHEEHTLVNALLHHCAGQLSGIGGVARPGIVHRLDKETSGCLVVAKNDETHVGL
                     SVQFATRKVEKIYQAILCGELPRDQGAIHAAIARHPSHRKRMAVDDDLGRVAWTDYRV
                     LERLRGATLVEAILHTGRTHQIRVHFQFLGFPVVGDATYGNRQNQRLADLTGYIAPRQ
                     MLHSGRLAFIHPRTGKRLSSEAPLPEDFLDALAALR"
     gene            complement(2235..2768)
                     /gene="lspA"
                     /locus_tag="VIL39_03475"
     CDS             complement(2235..2768)
                     /gene="lspA"
                     /locus_tag="VIL39_03475"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5480514.1"
                     /translation="MNPSNSLVRTPNRRIALIAMVVVALDQLTKQLVLRFLPQIGQER
                     VVIDGFFRFVHWGNTGAAWSLFRGNNEFLAGVAVLALLILFLSRHHFDSRTLLSQIAF
                     GLIFGGIVGNLIDRLRVRHVIDFIYFYLQPGGGTEIGFPAFNVADSAICTGVGLVFLI
                     TWQSDHKAKPVEPLPSK"
     gene            2890..3324
                     /gene="rplM"
                     /locus_tag="VIL39_03480"
     CDS             2890..3324
                     /gene="rplM"
                     /locus_tag="VIL39_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5480515.1"
                     /translation="MKTYLPKVNLEQRKWHVVDAKGAVLGRLAAQVADILRGKNKPVY
                     TPHLDAGDFVVVINAEKVVLTGKKETQKKFMSYSGWKSGDRYRTVEEVRAKQPEKLIT
                     HAVRGMIPKNRLGRVLLTKLKVYKGAEHPHSAQQPQTLAPAK"
     gene            3367..>3694
                     /gene="rpsI"
                     /locus_tag="VIL39_03485"
     CDS             3367..>3694
                     /gene="rpsI"
                     /locus_tag="VIL39_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5480516.1"
                     /translation="MSDIKLPEVKTPAHLGTGRRKSAVARVRLSTGTGKIVINGRPFE
                     NYFPMESLRGVVTQPLTVTGSTDKFDAQITVTGGGPAGQAGAVRHGIARALLTVDANL
                     RPVLKAE"
BASE COUNT          749 a         1154 c         1112 g          679 t
ORIGIN      
        1 ggagctaggg agcgttcgga cgttgctgcg gacaagaatg tccgcacgcc gattcggtgc
       61 aggctttgcc gcggagtgcc aagtgcgtcc agtgtcacca ctgggcccgg atttgctccg
      121 gggtgttccc ttccgcgcgc aggcggcgca ggctcagggc gtcatggcgt ttggccaggc
      181 ggactccttt ttcgtccgtc atcaatgggc agtgatagaa ttggggagcc ggtaccccga
      241 gcgcgcgata caccagcagt tgccgggcgg tggacgggag caaatccgcg ccgcgcacca
      301 cctcggtgat ccgcatcgcc gcgtcgtcca ccacgacaga aagctggtaa gccggaaccc
      361 catcgtgccg ccagaccaca aaatcgccga agtccttgcc cgcgacgaat tgctgcggcc
      421 cgcaatggcc gtcagtgaac gtaatcgtct cgccatccgg cacgcggaaa cgccagttcg
      481 ccttttgggg cgaaccgctc gtggggtttg ggtcgaacgc gcacgcctca ccgtctggct
      541 tgaacttctc gcggcaggtg cctggataca ccggctcgtc ttcgccctgg tgcggggcgg
      601 acagcgcccg ccgcacgtcc gaacgcgaac aagtgcaggg atagatgaac ccagcggcca
      661 gaagttttcg cattgcggcc aggtagaact cgcgccgctc gctctgattg tagggcccga
      721 acggcccgcc gcaatccggc ccttcctgcc attcaaagcc aaaccatttc aagtcttcga
      781 gcatggcgct gacaaactcc agcttgaagc gcttggcatc caggtcctca tttcgcaata
      841 cgagcacacc gccgctggcg cgggcgcgct gttgcgcaat ccagaaagtg cgcgcgtggc
      901 cgaggtgcag gtaacctgtc ggcgatggcg cgaggcggcc gcgatacatg atccgtgatg
      961 cgtgaatagc ttgtctcagg gtttccgctg caaccattgg catcggtctc actttattgc
     1021 gtcaaaggtc tgctgcagga tttgccagtc cttgaggccc ttgaaaacac tatcattctg
     1081 ccctagcgtg tcaaactcca accctgcttc ctctcgcggc ctcggcggct caccgcaggg
     1141 cggccagcgc gtccaggaaa tcctcgggca gcggcgcctc agagctcaga cgcttgcccg
     1201 ttcgtggatg gataaaagcg aggcggccgg agtgcagcat ctggcgtggc gctatgtacc
     1261 cagtcaggtc cgccagccgc tggttttgcc gattcccgta agtcgcatcg cccacaaccg
     1321 ggaaaccaag gaactggaaa tgaacccgga tctggtgcgt ccggccggtg tgcaggatcg
     1381 cctcgaccag cgtcgctccg cggagacgct ccagcacgcg gtagtctgtc cacgccacgc
     1441 gtccgagatc gtcgtccacc gccatgcgct tgcggtggga gggatggcgg gcaatggcgg
     1501 cgtggattgc gccctgatcg cgcggcagct cgccgcagag gatcgcctgg tagatctttt
     1561 ccaccttgcg ggtggcgaac tgcacggaca accccacgtg cgtttcgtca ttcttcgcca
     1621 caaccaggca gccgctggtt tccttgtcga ggcggtgcac gatgcccggg cgcgcgacgc
     1681 cgccgatgcc gctcaactgc cccgcgcaat ggtggagcag ggcattgacg agcgtgtgct
     1741 cttcgtgccc agctcccgga tgcaccacca gcccgggcgg cttgttcagc accaacaggg
     1801 cctcgtcctc atataagatg ttgagcggca tcttttccgc ttgggcttcg gcggaccgcg
     1861 cttcaggcca gtgaacttcg acttgttcgc cagcccgcgg cgcatgcgtc ggcttcacgg
     1921 tccgcccgtt gacccggatg tagccctgca caatcagccg ctgaattgcg ccgcgcgaga
     1981 cagccgggaa cttgccgcgc aggtacatgt cgagccgcac cgacggaagc gagtgctcga
     2041 tggtcagaac ttcagtgcga gaggacatga tgagaagtct gaagtccgcg ggccgaagtc
     2101 cgaagggcga agtccaaggt tcaacttgga aatcaggtgg tgaggaatcc ctgagttcct
     2161 gatctccaaa ctaaaggtcc aagatctgag gttcaaggta tcagtcgttg cggcctccca
     2221 actctcgatg gcactcactt ggaaggtaac ggttcgactg gcttggcctt gtggtcgctc
     2281 tgccaggtga tcaggaaaac cagcccgacg ccggtgcaga tggcgctgtc ggccacgttg
     2341 aaggcgggaa acccgatttc cgtccctccg cccggctgca agtaaaagta aatgaaatca
     2401 atcacatgcc gcacccgcag ccggtcaatg aggttgccaa caattccgcc gaagatcagg
     2461 ccgaaggcga tctggctaag gagggtgcgc gagtcaaaat gatgccggct caggaacaaa
     2521 atgagcagcg ccagcaccgc gactccagcc aggaactcat tgttgccgcg aaacaggctc
     2581 caagccgcgc cggtattccc ccaatgcacg aacctgaaga agccatcaat tacgacccgc
     2641 tcttgcccta tctgagggag gaaccggagc acgagttgct tggttaactg atcgagcgcg
     2701 accaccacca tagcgatcag cgcaatgcgg cgattcggcg tccggaccag cgaattcgat
     2761 ggattcatgc cagagatcga tcccggtata attagatgcc tcgcaaaaga caatgcaaaa
     2821 caattgaaaa gtctgttgac ttggccgctc cgtttgctat tttccagctt cctttgatca
     2881 agatttgaga tgaagacgta tttgccgaaa gtaaatttgg aacaacgtaa gtggcacgtg
     2941 gttgacgcca agggcgccgt cctcggccgc ttggcggcgc aggtcgccga cattttgcgg
     3001 ggaaagaaca agcccgtcta taccccgcac cttgatgccg gcgattttgt cgtcgtcatc
     3061 aacgccgaga aggtcgtcct cacgggcaag aaggagaccc agaagaagtt catgagctat
     3121 tccggttgga aaagcggcga ccgatatcgc accgtcgaag aggtccgcgc caagcagccc
     3181 gaaaagctca tcacccacgc tgtgcgaggc atgattccca agaaccggct cggccgggtg
     3241 ttgctgacca agttaaaagt gtacaaaggc gcggagcacc cgcactccgc gcagcagccc
     3301 cagacgctgg ctcccgccaa ataaagctgc cgaccattga ctatcaacca tcaacttttc
     3361 ccaggcatgt ccgacataaa gctccccgaa gtcaaaactc ccgcacacct tggcactggc
     3421 cgccgcaagt ccgcggtggc gcgcgtgcgc ctctccaccg gcaccggcaa gatcgtgatc
     3481 aatggccggc cgttcgagaa ttatttcccg atggaatcgc tgcgcggggt ggtgacccag
     3541 ccgttgactg tcaccggctc gaccgacaag ttcgacgcgc agatcaccgt caccggcggc
     3601 ggcccagccg gccaggctgg cgcggtgcgc catggcatcg cccgcgcgtt gctcacggtg
     3661 gacgccaacc tgcggcccgt gttgaaggcc gaag
//
LOCUS       DATCGS010000173         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_263547, whole genome shotgun sequence.
ACCESSION   DATCGS010000173 DATCGS010000000
VERSION     DATCGS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263547"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VIL39_03490"
     CDS             complement(<1..337)
                     /locus_tag="VIL39_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY5480517.1"
                     /translation="MFDECVIRGRRFAPARFCAPLVGYTHSAFRRLVAELGGCGAVWT
                     EMLAARQILSENFRTSPWLRRRSEEGCVIYQLMLGPGDPLERIVDRLGEAGAEALDLN
                     LACGSRSARA"
     gene            complement(535..1242)
                     /gene="yihA"
                     /locus_tag="VIL39_03495"
     CDS             complement(535..1242)
                     /gene="yihA"
                     /locus_tag="VIL39_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY5480518.1"
                     /translation="MNISSAIFDCSAPDLDSCPDESLPEFAFIGRSNVGKSSLLNMLA
                     GEQGLARVSTTPGFTKLINIFTMNKCWRLVDLPGYGFAHGARKHRARFNEAVADYLEN
                     RPNLCLVFALIESGLAPQGIDLEFVEWLVRHAVPFVLVFNKTDKVSAATVQTNIAAFT
                     DAISAWFEKLPAVFTCSAATRQGRQELLGVIDEEMMAINATSIRARVPPESSPNLSME
                     ARVRENRKRRPDLNRPW"
     gene            1501..2712
                     /locus_tag="VIL39_03500"
     CDS             1501..2712
                     /locus_tag="VIL39_03500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5480519.1"
                     /translation="MATVSIPQLRLRVNATAETLIRSRHPWLFANSIREENRSGRLGE
                     LAVIFDRNDRFLAIGLFDPESPIRVRVLHTGKPATIDSAWWQARLKQTLARREGLFDA
                     QTNGYRCIHGESDGWPGLVLDRYDTTFVLKLYTAAWLPRLDEVVGSIRAALHPQRLVL
                     RLSRNIQSSAKEQGPCNDGQLLLGEKPHGPLTFLESGLRFEADVLRGQKTGFFLDQRE
                     NRRCVESLARGRNVLNAFSFSGGFSLYAARGGARSVTDLDISEHALAGARRNFALNQA
                     APGMAACRHDCVRADAFEWLAAKPVRKFDLIVLDPPSLAKREAERAGAIQAYGRLAAN
                     GIRALQPGGVLVAASCSAHVSAMEFFGAVRSAARRSGQSFAELETTGHPPDHPATFAE
                     GQYLKCIYLRC"
     gene            complement(3084..4349)
                     /locus_tag="VIL39_03505"
     CDS             complement(3084..4349)
                     /locus_tag="VIL39_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEY5480520.1"
                     /translation="MSKLLTLEKYSIGVGDRFAHQAKAQLQACILAAQQGVEVIPVWN
                     KSNREHLTIGSDPATTRAAADAAVKALHWQKPYHVDADHIRLDTVDRFLPYADFYTLD
                     VADSIGKPAEAKSAAEFAGRHGELIGRISIPGIEQPFQTTRAEIERTAAKYLLAVKEA
                     GKIYRRIAQAKGERTFITEVSMDETDSPQTPPELIIILAALADEGIPLQTIAPKFTGR
                     FNKGVDYVGDVAQFEREFNQDLAVIAFAVKQYGLPATLKLSLHSGSDKFSIYAPIRRG
                     LARFGAGLHVKTAGTTWLEELIGLAEAGGSGLALAREIYASALEHVGELCAPYASVIN
                     IDRAQLPAAATVNGWSAAQFVSALRHDPGHKAFNPHLRQLLHVGYKIAAQMGDRYLEA
                     LQTHADSISRNVTANLYERHLKPLFLNRG"
BASE COUNT          880 a         1439 c         1401 g          860 t
ORIGIN      
        1 acgcgcgcgc cgacctcgag ccgcaggcca ggttcaagtc cagcgcctcc gcccccgcct
       61 ctcccagccg atccacaatc cgttccaatg ggtcacccgg tcccagcatg agctgataga
      121 tcacgcaccc ttcctcggag cggcggcgca gccagggcga cgtccggaag ttctcgctca
      181 ggatctgccg cgccgccagc atctccgtcc acaccgcgcc gcagccgccc aattccgcca
      241 ccaaccgccg gaaggcactg tgcgtgtaac cgaccagcgg cgcgcaaaag cgcgccgggg
      301 caaaacgccg gcctcggatg acacactcat cgaacaacgc cccacagggt agcccggtgg
      361 caggccccag cccaagacat tacgggcagt tttgaggaag gttttctcgc gcagacgaac
      421 tacgcatcag ccctgcgctg gtgtaatacg ctgctcgctc aagtgcccac caatccccag
      481 acactcgatg aaccgccgga tttccggtat caactcaatc tgatagccac gacctcacca
      541 agggcggttg aggtcgggac gccgctttcg gttttcccga acgcgcgctt ccatgcttag
      601 gtttggtgag ctttcgggag gtacccgtgc ccgaattgac gtggcgttta ttgccatcat
      661 ctcctcatca attaccccga gcaactcttg ccggccctgc cgagtcgcgg ctgaacaggt
      721 gaacactgcc ggcagctttt cgaaccacgc cgagatggca tcggtaaagg ctgcgatgtt
      781 tgtctgcacc gtggcggcgg ataccttgtc ggtcttgttg aagacgagga cgaacggcac
      841 ggcgtggcgc acgagccatt ccacgaactc gagatcgatt ccttgcggcg ccagaccaga
      901 ctcgattagc gcaaagacca ggcagaggtt cgggcgattc tccaagtaat cggccacggc
      961 ttcgttgaac cgggccctgt gcttccgggc accgtgggca aagccatatc caggcagatc
     1021 caccagccgc cagcatttgt tcatcgtgaa gatgttaatt agtttggtga accccggcgt
     1081 ggtcgagacg cgggccaatc cctgctcgcc cgcgagcatg ttcagcagcg aggacttgcc
     1141 gacgttcgag cggccgatga aggcgaactc cggcagcgac tcgtccggac acgaatccaa
     1201 atccggcgcg ctgcaatcaa aaatggcgga tgaaatattc acgatggaaa actcacagcc
     1261 cattgtcgca caaatccacc gtattgatac ccatattgtc agaaaagggg ttgttcaatc
     1321 cttctggctg aacacgcgat gagccccccg gctcatgacg tggtagatcg caccgggata
     1381 ctccactcgc attttgcgcg gcatcggcga gagactggcg ctacccggct aaaacgtcaa
     1441 acagaaccgc caaactatgg gttgacccct tttccgaact tggcgcaagc tgtgggaaag
     1501 atggcaactg tgtccattcc acaattacga ctgcgcgtca acgcaaccgc ggaaaccctc
     1561 atccgctcgc gtcatccgtg gctgtttgcc aacagcatcc gcgaggaaaa ccgctcgggc
     1621 cgactgggcg aacttgcggt cattttcgat cgtaacgaca ggtttcttgc catcggtcta
     1681 tttgatcccg aatcacccat ccgcgtccgg gtgctgcaca cgggcaagcc ggcgacgatc
     1741 gacagcgcct ggtggcaagc gcggttgaag caaacgctgg cgcgccggga gggattgttc
     1801 gatgcgcaaa ccaacggcta ccgctgcatc catggcgaaa gcgacggctg gccgggtctc
     1861 gtcctcgatc gttacgacac gacctttgtg ctgaagcttt acaccgccgc gtggttgccg
     1921 cgcttggatg aagtcgtggg cagcattcgg gcagccctgc acccgcagcg gctcgttctc
     1981 cggctcagcc ggaatattca atcctccgcc aaagaacagg gtccctgcaa cgacggccag
     2041 cttctactcg gtgaaaagcc tcacggcccg ttgacgtttc ttgaatccgg cctgcgcttc
     2101 gaagcggatg tgctgcgggg acagaaaacc ggcttcttcc ttgatcaacg cgagaaccgc
     2161 cgctgcgtcg agtcgttggc gcgcggccga aatgtgctga atgccttcag tttttcgggc
     2221 gggttttcgc tttatgccgc acgaggcggg gcaagatcag tcaccgatct cgatatcagc
     2281 gagcacgcgc tcgccggagc gcggcgcaat ttcgcactca atcaagccgc tcccggcatg
     2341 gcggcctgtc gccatgattg tgtccgggcg gacgcctttg aatggctggc ggcaaaaccg
     2401 gtgcgtaagt tcgacctgat cgtgctcgat cctccctctc tcgccaaacg cgaagccgag
     2461 cgcgctggag cgatccaggc ctacggacgg ctggcggcca acggcattcg ggcactgcag
     2521 cctggaggcg tgctggtggc cgcttcctgc tcggcgcacg tttcagcgat ggaattcttc
     2581 ggtgcagtgc ggtcggcggc ccggcggtcg gggcagagtt tcgccgagtt ggagacgacc
     2641 ggccatccgc cggaccaccc ggcaacgttc gccgaggggc agtatctgaa gtgcatctac
     2701 ctccgctgct agacggcgag ggtaaagaac tgcctcgctg ccgccagcat cctggtacca
     2761 gggtatgtcc agggcgcccc cagaccaccc ccaggaaggc gcgggagggg agttgtattc
     2821 ggtatgctaa tgggaaacac tgaactttga acctcgaacc ttgaaccttg aaccttgaac
     2881 agccgggcaa agccacccaa agccacccca aggccacccc aaggcttgtt ggtagccaac
     2941 tgataggcac cccaaagcca ccccaaggct accccaaggc taccccaaag ccaccccaag
     3001 gccaccccaa ggccactcca aggccacccc aaggccaccc caaggctacc ccaaggctac
     3061 cccaaagcca gggagcccgg aatctatccc cggttgagga acagcggctt caggtggcgt
     3121 tcgtagagat tggcggtgac gtttctggag atggagtcgg cgtgggtctg tagggcttcc
     3181 aggtaacggt cgcccatctg ggcggcaatc ttgtagccaa cgtgcagcag ttgccgcagg
     3241 tgggggttga atgccttgtg gcccgggtcg tgccggaggg cggagacgaa ttgcgccgcg
     3301 ctccaaccgt tgacggtggc ggcggcagga agttgcgccc ggtcaatatt gatgaccgag
     3361 gcgtaggggg cgcagagttc gcccacgtgc tcgagggcgc tggcatagat ttccctggcc
     3421 aaggcgaggc cggagccacc cgcttcggcc agaccgatga gttcttcgag ccaggttgtg
     3481 ccggcggtct tgacgtggag accagcgcca aagcgggcca acccgcgccg gatgggagca
     3541 tagatggaga acttgtcgct gccggagtgc aggctgagct tcagcgtggc gggcagtccg
     3601 tattgcttca ccgcaaacgc aatgacggcg aggtcctggt tgaactcgcg ctcgaattgg
     3661 gcgacatcgc cgacgtaatc aacgcccttg ttgaagcggc cggtgaactt cggtgcgatg
     3721 gtctgcaagg gaatgccctc gtcggcgagc gcggcaagga tgatgatcag ctcagggggc
     3781 gtctgggggc tgtcggtctc gtccatcgaa acttcggtga tgaacgtcct ctcgcctttg
     3841 gcctgggcaa tgcggcggta tatcttcccc gcctccttga ccgccagcag gtatttggcc
     3901 gccgtgcgct cgatctcggc gcgggtggtt tgaaacggtt gctcgatgcc cgggatcgag
     3961 atgcgcccga tcagctcgcc gtgccggccc gcgaactccg cggcggactt ggcctcggcg
     4021 ggcttcccaa tcgagtcggc cacgtcaagg gtgtagaagt ccgcataggg cagaaagcgg
     4081 tccacggtgt cgaggcggat gtggtcggcg tccacgtggt aaggcttttg ccagtgcagg
     4141 gccttgacgg cggcatcggc cgcggcgcgg gtcgtggcgg gatcggagcc gatggtgagg
     4201 tgttcgcggt tggacttgtt ccagaccgga atcacctcga cgccttgctg cgccgcgagg
     4261 atacaggcct ggagctgggc tttggcctgg tgcgcaaagc ggtcgccgac tccgattgaa
     4321 tacttctcaa gcgtcaagag tttgctcata aatcagactt tcgctggtca ggcggagagt
     4381 agccgaggtt ggtggcagag gaaaccctga tcttgcgggg gcactatcgg ttgccactcc
     4441 cccgctgact tggtaacctc ctctcatgat cagacatggg attcccgctc tcagtcttcg
     4501 cgccttcgcg ccttcgctgt tcctctctct tctcgctgtc ccgaccgcct tcgcactgga
     4561 cgccaccgac atcccagccg
//
LOCUS       DATCGS010000174         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_610228, whole genome shotgun sequence.
ACCESSION   DATCGS010000174 DATCGS010000000
VERSION     DATCGS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610228"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VIL39_03510"
     CDS             <1..1513
                     /locus_tag="VIL39_03510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5480521.1"
                     /translation="IAFAHPNVRYFRHEKNLGLSVARNTGIAAATGEIIAFTDSDCRA
                     DEDWLYYLVGSLLGSDFAGMGGPNLLPPEDSLVAAAVMASPGGPAHVMLTDRQAEHIP
                     GCNMAFHKWALEQVGGFDPIFHTAGDDVDLCWRLQQAGLRIGFSPPAFVWHYRRPAIG
                     AYLRQQRGYGEAEALLVRKHPECFNSFGGSLWRGRIYTTAKLGDLIRPPIIYRGLFGS
                     AGFQFLYASEPAFNLMVTTMLEYHVLVTLPLLVVSVIFHPLLPVALTSLLISVGVCVA
                     AGAQAALPRNKRRWWSRPLVGMLFFLQPIVRGWARYQGRLMQRPAALAAHQTLDSIAL
                     RDSKQPLREVQYWAEQRLDRLALVADILRRLDQQGWPNKSDIGWSDYDVEVYDTRWSK
                     LQLTTVAEEHPRTKQLIRCRLRARWALRARVAFWSLCGFELLVCGLVGHWFPWLWLLL
                     VTLPLFCWFLRHEQRNLRSMITVFLDEMAKEWHLTKVEPQSARQPASEPQAAP"
     gene            1559..2353
                     /locus_tag="VIL39_03515"
     CDS             1559..2353
                     /locus_tag="VIL39_03515"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5480522.1"
                     /translation="MIQYLDLLRHVLEHGKFKSDRTGTGTYSVFGAQARFPLAQGFPL
                     LTTKKVHLKSIVYELLWFLRGDTNTKYLHEHGVTIWDEWADKDGNLGRIYGAQWCDWR
                     APDGRSINQIDQVIAQIQKQPDSRRLIVSAWNVGELDQMALAPCHALFQFSVQGGELN
                     CQLYQRSADLFLGVPFNIASYALLTMMVAQVCKLKLGTFVHTFGDLHLYVNHLDQARL
                     QLPRQPRPLPQMKLNPAVKNIHDFQYADFELTGYNPHPGIKAPIAV"
     gene            2350..>2474
                     /locus_tag="VIL39_03520"
     CDS             2350..>2474
                     /locus_tag="VIL39_03520"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY5480523.1"
                     /translation="MKPFKAIAAMSLNRVIGAGNRIPWHLPEDFKWFKRMTTGQV"
BASE COUNT          443 a          791 c          746 g          494 t
ORIGIN      
        1 aatcgccttc gcgcatccca atgtccgcta cttccgccac gagaagaacc tcggcctctc
       61 cgtcgcgcgc aacacgggca tcgccgcggc gaccggtgag atcatcgcgt tcacggactc
      121 cgattgccgg gcggacgagg actggctgta ttacctcgtg ggcagccttc ttgggagcga
      181 tttcgcgggc atgggcggcc ctaacctcct gccgccggag gactcgctgg tagccgcagc
      241 ggtcatggct tcgccgggtg gaccggcgca cgtgatgctg accgaccgtc aggctgagca
      301 cattcccggc tgcaacatgg cgttccacaa atgggcgctg gagcaggttg gcgggttcga
      361 tcccattttc cacacggcgg gcgacgatgt ggacctatgc tggcgcctgc agcaagcggg
      421 cttgagaatc ggtttcagcc cgccggcctt cgtctggcat taccgccgtc cggccattgg
      481 cgcctacttg agacagcagc gcggttacgg cgaagcggag gccctgctgg tgcgcaagca
      541 cccggaatgc ttcaattcat ttggcggcag cctctggcgc ggccggattt acactaccgc
      601 caagctaggc gatttgattc ggccgccgat catttaccgg ggcctgttcg gcagcgccgg
      661 cttccaattc ctctacgcat ctgagcccgc cttcaatttg atggttacca ccatgctcga
      721 gtatcacgtg ctggtgacgc tgccgctgtt ggtggtgtcg gtgatattcc atcccctgct
      781 gccggtggcc cttacgagtc tgctgatctc cgtcggggtg tgcgtcgcgg caggcgcgca
      841 agccgccttg cccagaaaca aacggcgctg gtggtcgcgc cccctggtgg gcatgctctt
      901 tttcctgcag ccgatcgtgc gcggctgggc gcgctaccag ggtcgcctga tgcagcggcc
      961 cgcagcgctg gcggcccacc agacgctcga ttccatcgcg ctgcgggaca gcaagcaacc
     1021 gttgcgcgag gtccaatact gggccgagca gcgcctcgac cggctggcgc tggtggccga
     1081 tattctgcgc cggctggacc agcagggctg gccgaacaag tccgacattg gctggagcga
     1141 ctacgacgtg gaggtgtacg acacgcgctg gagcaagctg cagctcacca ccgtcgcgga
     1201 agagcatccc cggaccaaac aactgatccg ctgccgcttg cgcgcgcggt gggctttgcg
     1261 ggccagggtc gccttctggt ccttgtgcgg gtttgaactg ctcgtctgtg gtcttgtcgg
     1321 gcactggttc ccgtggttgt ggttgttgct ggtaaccctg ccgctgtttt gctggtttct
     1381 ccgccatgag cagagaaact tgcggagcat gatcaccgtg ttcctggatg agatggccaa
     1441 agaatggcac ctgaccaaag tggaaccgca gagcgcccgc caacccgcat cggaaccgca
     1501 agctgcaccc tgattgcgtc cgtgcgacca aagcggtagt ctgaagtccg gcattgctat
     1561 gattcagtat ctagacctgc tgcgccatgt actggagcac gggaaattta agagtgaccg
     1621 caccggcacc ggcacctatt cggtgttcgg ggcccaggcg cgttttccac tcgcgcaagg
     1681 gttcccgctg ctcacgacca agaaggtcca cctcaaatcc attgtttacg aactgctctg
     1741 gttcctccgc ggcgacacga acaccaaata cctgcacgag cacggggtaa ccatctggga
     1801 cgagtgggcg gacaaggacg gcaacctggg ccggatttat ggcgcgcaat ggtgcgattg
     1861 gcgcgccccc gatggccgct ccatcaacca gattgaccag gtcattgctc aaatccagaa
     1921 gcagcccgac agccgccggt taatcgtgag cgcttggaac gtcggcgagc ttgaccaaat
     1981 ggcgctggca ccctgccatg cgttgtttca gttttccgtc caggggggcg agctgaactg
     2041 ccagctctac cagcgcagcg cggacttgtt cctcggtgtg cccttcaaca tcgcctccta
     2101 cgcactcctc acgatgatgg tcgcgcaggt ctgcaagctg aagctcggca cgttcgtcca
     2161 cactttcggc gacctgcacc tttacgtgaa tcatctcgac caggctcggc tgcagctccc
     2221 ccgccagccg cgcccgctgc cgcagatgaa actgaacccg gcagtaaaga acatccacga
     2281 tttccaatac gccgattttg agttgaccgg ctacaacccg catcccggca tcaaagcccc
     2341 cattgccgta tgaaaccttt caaagccatc gccgccatgt cgctcaatcg ggtgattggc
     2401 gctggcaacc gcatcccgtg gcatctgccg gaggatttca agtggttcaa acgaatgacc
     2461 accggccagg ttat
//
LOCUS       DATCGS010000175         3678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_115695, whole genome shotgun sequence.
ACCESSION   DATCGS010000175 DATCGS010000000
VERSION     DATCGS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115695"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1739
                     /locus_tag="VIL39_03525"
     CDS             279..1739
                     /locus_tag="VIL39_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY5480524.1"
                     /translation="MAVQNRIVKVQKRNRALVKFDAARIRKAILGAAESIGGFQQDLV
                     PGVNETIFNAFGTADRIAEFLADAVVVCLNSDPHHLISNFPPTIETIQDEVLHALHSY
                     GFQNTADAYSCYRWGRHWLREGAIAADKFAGNGFPHERMKQTLAWNRQRGCDTVAGLN
                     EIVRSGKIKPLVDESLALYEQALDEGAKKVIGRLNAGDKLRVMWVSGPSSSGKTTTTV
                     KLTERLQKQGLRFLMLNLDDYFWSLVEHPTDWINDRNYETPEALDIQLLNQHITGLLN
                     GETIEKPIYSFKEGRRVATKPIKLEPGQILLLDCLHGLYPPITEGIPASSQFRLYIET
                     QNVLYEGDGSSGRRTHFTDIRLLRRMLRDAQHRNHSPLLTILHWHYVRAGELFSIIPL
                     SGLADHVVNGGFPFDLPVLKPFFCGTDGYLPRPEDLEAYPGFLDARIRLGRVKHLLES
                     VEGLSKEQLRTCDIIPGDAVIREFIGGSTIKIPHNE"
     gene            1752..2234
                     /locus_tag="VIL39_03530"
     CDS             1752..2234
                     /locus_tag="VIL39_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY5480525.1"
                     /translation="MKTFLVLVFGVALGAAAVWFYSTHRDDPRMRSAEQKVENATKTT
                     RDAAQDKLRALHLSSVDIKDELARTGQVVRRKASEAGHAIADATADTRITAAIKGKLL
                     ASRDLSALNISVNTSAGVVTLSGFVSSSEHISKAILLAMETDGVREVVSTLQVKPKAK
                     "
     gene            complement(2373..3038)
                     /gene="infC"
                     /locus_tag="VIL39_03535"
     CDS             complement(2373..3038)
                     /gene="infC"
                     /locus_tag="VIL39_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5480526.1"
                     /translation="MSRPFPSRSYHSREPLHRRNGKIRSREVRVLDENKQQLGVMPLN
                     EALRLAMSKGLDLVEIAPNATPSVCRIVDYGKFMYEEAKKNKDAQGRQSGSKMKELQL
                     SPVIDPHDFGIKLGHAIGFLCEDMKVRVKLRFRGRQKAHKEFGFEVVNRFVNAAAAWS
                     QPDSPPKMLGDRDLNVVLSPLPRNKRAKNPHLEETAEPPEAPEEQEAPESAEAPTAPD
                     SAS"
     gene            3329..>3678
                     /locus_tag="VIL39_03540"
     CDS             3329..>3678
                     /locus_tag="VIL39_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY5480527.1"
                     /translation="MGLKLFNTLSHAVDEFVPLDPAGKSVGMYCCGPTVYDFAHIGNW
                     RTFIFGDLVRRYLAFKGYAVTQVMNITDVDDKIIKRVRETGTPLREFTGKFEAAFFDD
                     LKTLNCRLPQQTPRA"
BASE COUNT          702 a         1206 c         1029 g          741 t
ORIGIN      
        1 cgctcagggt cggctccgat ggccgcgttc ccatcggcgc ccagcgcctc cgggtcgaac
       61 gtcccccggg caccaaggtg gtgctgtgcc tccatcccag cggccatcac tccgtcctgg
      121 ctgcgtcacc cgataaacaa gagaaaccgg tactcctttt tactaatcgc cagaaatgaa
      181 cctctcctgg tttgctaact acagaaaatt cctggtttga aaactacgcg acacaaactg
      241 cattgtcagc cgcagggtct ggtgtatagt gatagctcat ggcagttcaa aaccgcattg
      301 ttaaggtcca gaagcgtaat cgcgcactgg tgaaattcga tgctgctcgc attcggaagg
      361 cgattctggg cgcggcggaa tccatcggcg gcttccagca ggacctggtt cccggcgtca
      421 acgagacgat cttcaacgcc ttcgggactg ccgacagaat cgccgagttc ctggccgacg
      481 cggtggtggt ctgcctcaat tccgatcccc accacctgat ttccaacttc ccgccgacca
      541 tcgagaccat ccaggacgag gtgctgcatg cgttgcacag ttacggcttc cagaataccg
      601 ccgacgccta ttcatgctat cggtggggcc ggcattggct gcgggaaggc gcgattgccg
      661 ccgataagtt tgccggcaac ggctttcccc acgagcggat gaagcagacg ctggcctgga
      721 accggcagcg tggctgcgac accgtggccg gcctgaacga aatcgtccgc agcgggaaga
      781 tcaaaccctt ggtggacgaa tcgctcgccc tctacgagca ggcgctggat gagggggcca
      841 agaaggtaat tgggcgcctc aatgccgggg acaagctccg cgtgatgtgg gttagcggcc
      901 ccagttcttc gggcaagacc accaccaccg ttaagctcac cgaacgcctt cagaagcagg
      961 gcctgcgctt tctcatgctc aaccttgacg actatttctg gtcactcgtc gagcatccga
     1021 ccgactggat caacgaccgt aactacgaaa cgcccgaggc gctggacatc caattgctca
     1081 accagcacat caccggcttg ctcaacgggg aaacgattga gaagccgatc tacagcttca
     1141 aagagggccg ccgtgtcgcc accaagccca tcaagctgga gcctggccag atcctcctgc
     1201 tcgattgcct tcacgggctc taccccccca tcactgaagg catcccagcc tcgtcccagt
     1261 tccgtctcta catcgagacc cagaacgttc tttacgaagg tgacggcagc agcgggcggc
     1321 gcacgcactt caccgacatc cgtttgctcc ggcgaatgtt gcgtgacgcg cagcatcgca
     1381 accacagccc gctcctcacc atcctgcact ggcattacgt gcgcgccggt gagctcttca
     1441 gcatcattcc cttaagcggt ttggccgatc acgtcgtcaa cggcggattc ccctttgacc
     1501 tgcccgtcct caaaccgttc ttctgcggca cggacgggta cctgccccgc cctgaggatt
     1561 tggaggccta ccccggcttc ctggacgctc gcatccgctt gggccgggtg aagcatctgc
     1621 tggaatcggt cgagggcctc agcaaggagc agctccggac ctgcgatatc attccgggcg
     1681 atgccgtgat ccgcgaattc atcggcggca gcaccatcaa gattccacat aacgagtaaa
     1741 cctgtccgcg catgaaaact ttcctggtct tggtattcgg cgttgctctc ggggctgcgg
     1801 cagtctggtt ctacagcacg caccgggacg atccccgcat gcggtccgcc gaacagaagg
     1861 tcgagaacgc caccaagacg acccgcgacg ccgcccagga caaactccgc gcgctgcact
     1921 tgagctccgt ggacatcaag gatgagctgg ctcgcaccgg tcaggtagtg cgccgcaagg
     1981 cgagcgaggc tggccatgcg attgctgatg ccacagccga cacgcgcatc accgccgcca
     2041 tcaaaggcaa gctccttgcc agccgcgatc tgtccgcgct taacatctcg gtcaacacca
     2101 gcgccggagt ggtcacgctg tcgggctttg tgagttcctc cgagcacatt agcaaagcca
     2161 ttctgctggc aatggagacc gatggcgtgc gcgaggtggt atccaccctc caggtgaagc
     2221 cgaaggctaa gtaaataccc gttttgttgg aaggaacgcg ttctcccgcg tcacacttca
     2281 tcggcaagat ctcagggcat ggtggattcc gtccccgcct gcacaactac gaacaccctc
     2341 gggttgaggg tgcagccgca gatcgccggg cgctaagaag cagaatccgg cgcagtcggc
     2401 gcttctgccg actctggcgc ttcttgctct tctggcgcct ccggcggctc cgccgtctcc
     2461 tccaggtggg gattcttggc gcgcttgttg cgcggcagcg ggctgaggac gacgttcagg
     2521 tcgcggtcgc ccagcatctt cggcggcgaa tccggctggc tccacgcggc ggcggcgtta
     2581 acgaatcggt tcaccacctc gaagccgaat tccttgtgcg ccttctgccg cccgcgaaaa
     2641 cgcagcttga cccgaacctt catatcctcg cacaggaagc cgatggcatg gcccagtttg
     2701 atcccgaaat cgtgcggatc aatcaccggc gagagctgta gctccttcat tttgctcccc
     2761 gactggcggc cttgcgcgtc cttgttcttc ttggcctcct cgtacatgaa cttgccgtag
     2821 tccacgatcc ggcacactga cggcgtcgca ttgggcgcga tctcgaccag gtccagcccc
     2881 ttgctcatgg ccaggcgcag cgcctcattg agcggcatga cgccgagttg ttgcttgttc
     2941 tcgtccagca cgcgtacttc gcgggaacgg atcttcccat tccggcgatg cagcggctcg
     3001 cgggagtggt acgaacgcga gggaaagggg cggctcaacg agccctcccc ggtcggaatt
     3061 tgttcatgcg gctatagtct gccaaacttg ctcgaactca gttatgaata ccattacccc
     3121 tgcattttcg catttccccg ccatctggca agcccaattt gacgcgccca cggcagggcc
     3181 aaaacccgct tcaaatataa tctcgctcgt cccccagctc caatcccggt gctcttcgta
     3241 tttggaatcc gggtttcccc ctggcgtctc cgcggacttt agccttttct ttccccggct
     3301 cgcccctaag cttcagcgcg ttcgttgtat gggtttaaag ctgttcaaca cactgtcaca
     3361 cgcggtggac gagttcgtcc cgctggatcc ggccgggaag tcggtcggca tgtactgctg
     3421 cggcccgacc gtctatgact tcgcgcacat cggcaattgg cgcacgttca tcttcggcga
     3481 cctggtgcgg cgttatctgg ccttcaaagg ctatgccgtc acgcaggtga tgaatatcac
     3541 cgacgtggac gacaagatta tcaagcgcgt ccgcgagacc ggcacgccgc tccgggagtt
     3601 caccggcaag ttcgaggcgg cctttttcga cgacctcaag accctcaact gccgcctgcc
     3661 gcagcagaca ccgcgcgc
//
LOCUS       DATCGS010000176         4505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_709652, whole genome shotgun sequence.
ACCESSION   DATCGS010000176 DATCGS010000000
VERSION     DATCGS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709652"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1258
                     /locus_tag="VIL39_03545"
     CDS             218..1258
                     /locus_tag="VIL39_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY5480528.1"
                     /translation="MKLNFKRLDLQLTHTWTIARGSGTNISKTVVVELTGADGTVGLG
                     EAAPISRYKESIDTVETFFKGVDARGLSFNDVEGSMIYLDTLSPHDMSAKCALNLALL
                     DGAAKRNKKAVYDFLELGFRENHHVTSFTIGIDTPDIIRKKVLEAEKYPVLKVKVGVA
                     DDKVNLAALREVAPTKTVRVDANEGWKAKEQALEMIEWLAKDGHIEFVEQPMPAATPV
                     KDWVWLKQRSPLPIFADESYHLAKDVAQVAECFHGANVKLVKSGGVSAGYEALAAARK
                     AGLKTMLGCMIESSILISAAASLAELCDYLDVDGNILISNDPYVGVTAEKGILSFAAA
                     PEKYGLRVSPRA"
     gene            1490..2872
                     /locus_tag="VIL39_03550"
     CDS             1490..2872
                     /locus_tag="VIL39_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480529.1"
                     /translation="MQSMDDMALLREYAARNSEAAFETLVSRRIGLVYSAAMRQAGDP
                     HLAEEVTQTVFTILAQKAGKLSDKTILIGWLFQTTRFAALAQRRAAVKRQRHEQEVQM
                     QTELETAAPDPLWEQMSPLLDEALATLGEKDRQAVLLRFFENKSLGEVGISLGTGEDT
                     ARKRVSRALEKLHRYFNRRGISSTTPLIAGAISAYSVQPVSAALAKSVTVIAVAKGAA
                     AGGSTLTLIKGALKLMAWSKAKTAAVSLVIVSLATVSVIQHQAQVKLRDENQALRQQV
                     DGLQTSNAPVANPVAQETSSQALPDDQFRELLRLRGEVGVLRRQTNELANMLANARRS
                     QPRSPNSAAGQPPASALADDYPKTPEGATKSIFETMARGDWNALYTNFAEPGVPREVY
                     DQMFNDPVKSNYLAGLEVMSIGEPTNSFGSNMWFVPYKIRFKDGTEKEFRLHVAQDTR
                     SQRWIFKGGF"
     gene            complement(2948..3247)
                     /locus_tag="VIL39_03555"
     CDS             complement(2948..3247)
                     /locus_tag="VIL39_03555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5480530.1"
                     /translation="MNAKPLTVIAQFKAKPGKESLVRQELLSLLAPSRKDAGCLNYDL
                     HQAPDNPALFLFHENWTSKAHLDQHLQKPNLQAVLARVGQLVAEPPQITLWEKIG"
     gene            complement(3354..4382)
                     /locus_tag="VIL39_03560"
     CDS             complement(3354..4382)
                     /locus_tag="VIL39_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5480531.1"
                     /translation="MSTKTGESALRRHSQPARGNKGRRAKAPLPAGRGNSAVSTSGRS
                     PASSARDGNLGLLEALITSKLFRDYERAFTEATGLPIALRPVESWQLPLHGRQNEGPF
                     CALMAERSRTCGSCLAVQEKLTSAATREPATAVCPAGLSETIVPVRLGERLIGFLQTG
                     QVFRNEPTQSQFERTARLLAEWGVEVDRVALRQTYFSTRVVPARQQEAVIKLLSIFAQ
                     HLSMLSNQLLVHHQNAESPVITRAKAYIQEHQTDDLHLGQVAKAVNTSLFYFCKIFKK
                     ATGLNFTDYLSRIRIEKSKNLLLNPNLRVSEIAFEVGFQSLTHFNRVFKRAVGQSPTH
                     YREQLLGH"
BASE COUNT          871 a         1328 c         1424 g          882 t
ORIGIN      
        1 cggtcggttt ggtcataggc ttcttgctgt cgttcatagg ccgggcgtgt cggtgatcta
       61 ttgttctgcc gcatcatctc ggcacgcccc gccccccgtc aatcttctaa tgcccccgcc
      121 tccgggcacg ccgccgctct ccgccgctga ccggtagccg cgactcgcca aaactacggt
      181 gttgcatcct gggctggttt agggttttgt ttgcgccgtg aaactgaatt ttaagcgtct
      241 ggacctccag cttactcaca cgtggactat tgcccggggc agcggcacga acatctccaa
      301 gaccgtcgtc gtcgaactga ccggcgccga cggcaccgtc ggcttgggcg aagccgcgcc
      361 catctcgcgc tacaaggaat ccatagatac cgtggagaca ttcttcaagg gggtggacgc
      421 gcgcgggctc tcgttcaatg acgtcgaagg cagcatgatt tacctggaca cgctttcgcc
      481 gcacgacatg tcggccaagt gcgccctgaa cctcgccctg ctggacgggg ccgccaaacg
      541 caacaagaag gcggtctatg acttcctcga acttggcttc cgcgagaacc atcacgtgac
      601 ctccttcacc atcggcattg atacgccgga cataatccgc aagaaagtgc tggaggcgga
      661 gaagtatccg gttttgaagg tgaaggtggg cgtggccgac gacaaggtga acctggcggc
      721 attgcgcgaa gttgccccca cgaaaacggt gcgggtggac gccaacgaag gttggaaggc
      781 caaagaacag gcgctggaga tgatcgagtg gctcgcgaaa gatgggcata tcgagttcgt
      841 cgagcagccc atgcccgcgg ccacgccggt caaagactgg gtctggctca agcaacgctc
      901 gccgctgccg atcttcgccg acgaatccta tcatctcgcc aaggatgtgg cccaggtggc
      961 ggaatgtttc cacggcgcga acgtgaagct ggtcaagagc ggcggcgtca gcgccggcta
     1021 cgaggctctc gcggcggccc gcaaggcggg actgaaaacc atgctgggct gcatgatcga
     1081 gagcagcatt ctcattagcg ccgccgcgag ccttgccgag ttgtgcgact acctcgacgt
     1141 ggacggcaac attctcatct ccaacgaccc ttacgttggc gtcacagcgg agaagggtat
     1201 cctgtccttc gccgccgcgc cggagaagta cggcttgcgg gtcagcccgc gggcatagga
     1261 tgggctcagc agggattacg tcgctcgaat agacgagggc cagattccct cgtaggcaaa
     1321 ggccccggca ggggagctga ggttggcgag gcgtccgtcc aagtcccacc cccgtgccgt
     1381 tggtagaaga ggccgtgggc tgctgaaggc tccgcccccg tttcagagcc acaggctgag
     1441 gcacaaaaac attgtccggt tttccgccca acccgacatt tataggtgga tgcagtcaat
     1501 ggatgacatg gccctgctgc gagaatatgc agcgcgcaat tccgaagcgg ccttcgagac
     1561 gctggtttcg cgccgcatcg gcttggtgta ttcggcggcg atgcgacaag cgggcgatcc
     1621 gcatctcgcc gaggaagtca cgcagacggt gtttacgatc cttgcgcaaa aggccgggaa
     1681 gctgtccgac aagacgattc tgatcggctg gctgttccag accactcgtt ttgccgcgct
     1741 cgcacagcgg cgcgcggcgg tcaaacgcca gcgccacgag caggaggttc aaatgcagac
     1801 cgaactggaa accgccgcgc ccgaccccct ctgggaacaa atgtcgccgt tgctggacga
     1861 ggcgctggcc acgctgggcg aaaaggaccg gcaagccgtg ctgctgcgat tctttgaaaa
     1921 caagagtttg ggcgaggttg gcatctcact gggcaccggc gaggataccg cgcggaaacg
     1981 cgtcagccgc gccctcgaaa agctgcaccg ctattttaac cggcgtggca tttcctcgac
     2041 cacgcccctc atcgctggag cgatttccgc ttattccgtt caacctgtct ccgctgcgct
     2101 ggcaaagtcg gtaaccgtga ttgcggtggc caaaggcgcg gcggctggcg gctcaaccct
     2161 aaccctcatc aaaggagcat tgaaacttat ggcatggtca aaagcaaaga cggcggccgt
     2221 cagcctcgtt atcgtcagcc tggcaacggt gtcggtgatt cagcaccagg cgcaggttaa
     2281 actgcgcgac gagaaccagg cgttgcggca acaggtggat ggtttgcaga ccagcaacgc
     2341 gcctgtcgcc aacccggtag cccaggagac cagctctcaa gcccttccgg atgatcagtt
     2401 tagggaactg ctgcggctgc ggggtgaagt cggagtgttg cgccggcaaa ccaatgagct
     2461 cgcaaacatg ctggccaatg cgcggcggtc ccagccgcgg agcccaaact cagcggctgg
     2521 ccaaccacca gcgtcggcat tggcggatga ttatccgaag actccagaag gcgccaccaa
     2581 gagcatcttc gaaaccatgg cgcgaggtga ttggaatgcg ctctacacaa acttcgcaga
     2641 accgggagtg ccgcgcgagg tttatgacca gatgttcaat gacccagtca aaagcaatta
     2701 cctggccggc ctggaagtca tgagcatcgg cgagcccacg aatagtttcg gttcgaatat
     2761 gtggttcgtg ccctacaaga tccggttcaa ggatggcacc gagaaagaat tccgcctgca
     2821 cgtggcacag gacaccagaa gccaacgttg gatcttcaaa ggcgggtttt agcatccaag
     2881 gcggcgggct gagggttggc gggcgaaagt cgtggggtgc ttcgcgtctt cgcgccttcg
     2941 ttgtcaatca gccgatcttt tcccagagcg taatctgcgg cggctcggca accagttggc
     3001 cgacccgcgc cagcacggcc tgcagattgg gcttttgcag gtgctgatcg aggtgtgctt
     3061 tgctggtcca gttttcgtga aagaggaaga gcgcggggtt gtccggcgcc tggtgcaagt
     3121 cgtaattcag gcacccggca tccttgcggc tgggggccag cagcgagagc agttcctgtc
     3181 ggaccaggct ttccttgccc ggcttggctt tgaattgcgc gatcacagta agaggttttg
     3241 cgttcatgga ggccaatcat cgcgccgacg ggccctggct ggcaatgcgc ttcttgcacc
     3301 ggccaatccg caaagggatc gggcgtggtc tcggcgggct cccggccgaa cgcctaatgc
     3361 cccagcagtt gttctcgata gtgggttggc gactggccga cggctctctt aaacacgcgg
     3421 ttgaagtgag tgagcgactg aaacccgact tcgaaggcaa tttcactcac gcgcaggttt
     3481 ggattcaaca ggaggttctt ggatttctcg atgcgaatgc gtgagaggta gtcggtgaag
     3541 ttcaggccgg tcgccttctt gaatatcttg cagaaataga acaggctggt attgaccgct
     3601 ttggccacct gcccaaggtg caggtcatcg gtctggtgtt cctgaatgta ggctttggcc
     3661 cgggtgatga cgggcgactc tgcgttctgg tgatgcacga gaagctggtt gctgagcatt
     3721 gagaggtgtt gcgcaaaaat gctcagcagc ttgatcaccg cttcctgctg ccgtgccggg
     3781 accacccgtg tactaaaata cgtctgcctc agcgcgacgc ggtccacctc gacgccccat
     3841 tcggccagca ggcgggcggt gcgctcgaac tggctttggg tcggttcgtt gcgaaacacc
     3901 tggccggtct gcaagaagcc aatcaagcgc tctcccaatc gcaccggcac aatggtttcg
     3961 ctcaggccag ccgggcagac ggccgtggcg ggctcccgcg tggcggcgct cgtcagtttc
     4021 tcctgcaccg ccaggcacga gccgcacgtg cggctcctct ccgccataag ggcgcaaaag
     4081 ggaccttcgt tttgcctgcc atggagggga agctgccacg actccaccgg gcgcagcgcg
     4141 attggcaacc cggttgcctc ggtgaaagcg cgctcatagt ctcggaaaag ctttgaggtg
     4201 atgagggctt ccagcaaacc caggtttccg tctctggcag agctggccgg ggatcggccc
     4261 gaggtggaca cggcgctgtt gccccgtcca gcgggcagag gtgccttagc ccttcggccc
     4321 ttgttccctc gggcgggttg gctgtgtcgc ctgagcgccg actcaccggt ttttgtgctc
     4381 acagtcgcaa tatatggcgg gcactgttgg gacggcaagt tatctttggg gctcccttgg
     4441 gtgcccggca tttgtggtgc tggacctccg gtcaggagct cacgtgccgt ggctggcgcc
     4501 gctgg
//
LOCUS       DATCGS010000177         5971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_211476, whole genome shotgun sequence.
ACCESSION   DATCGS010000177 DATCGS010000000
VERSION     DATCGS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211476"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VIL39_03565"
     CDS             complement(<1..1451)
                     /locus_tag="VIL39_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY5480532.1"
                     /translation="MKTLRLHLLGFVLCLAPLAALPASSPNSAAWQFEVAIHKNLAAP
                     PKDGRLFVILAKTNNPEPRLTLGRTGLDAPQVLACDLKGFAPGAVALVGQGTFAFPIT
                     NLARVPAGDYFVQALFDSNTDLRSPEAPGNLYSKPQKIHLDPGQDGTRKLELTEQIPA
                     DQLPAETAQIKFVKIQSKLLSEFHGRPIFLRAGIVLPRDYERDPSRRYPLLVRIAGLN
                     GRYTSVLGLMGEKSEFRQTWQADDTPRLILLQLDGAGPNGDPYYINSANNGPFGDALV
                     QELIPHVEARFRAIGQSRGRVLNGNSTGGWVSLALQVFYPDFFNGTWSSCPDPVDFRA
                     LELVNIYQDDNAYVNKYGNDRPSDRDPNGDVRLTMRREVGVENLLGRGNSYTLSGEQW
                     GEWNAAFGPRGAGGLPVPLWDPQTGRINHAVAEQWKKYDLRLVLEQNWPTLGPKLRGK
                     IHISAGEADAYFLNNAVHLLDKFLATANLPFEG"
     gene            complement(1448..2734)
                     /locus_tag="VIL39_03570"
     CDS             complement(1448..2734)
                     /locus_tag="VIL39_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480533.1"
                     /translation="MRRPSVLVIFLTVFIDLIGFGIIVPLIPSYSEHFGAHGVVIGVI
                     FASYSAMQFIFSPIWGRLSDRHGRRPILLISTAGAAASYVLFARSTGLENHTAALWLM
                     VVSRMFAGVCGGNITVAQAYIADITPPAERSKKMGLIGMAFGLGFILGPFIGAESLRH
                     WGDTGPGWCAAALCAANFLLALFILTESHKPDSDQAAPRPHLDQWAHTLREPKVGFLV
                     IVFFLATFCFSCFESTLPLLIGTNFHLDFVRDATSASTVGYLFVYCGLIGALVQGGAI
                     GRLVKRMGEPRLIALSLVLTAASFAWIPFIKGASQLSWKVLFQHDGLPWVWLLMALAL
                     LSVGTSLTRPPLFGLLSNLTPDNEQGATIGVAQGAGSLARILGPVFATALLSWSPPLP
                     YLICAAVLLGTTVLVSRRLSRDSQPAVDSQAANLAK"
     gene            complement(2948..3589)
                     /locus_tag="VIL39_03575"
     CDS             complement(2948..3589)
                     /locus_tag="VIL39_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY5480534.1"
                     /translation="MKSLLRKMVPALLLISLMTSSALAQGRVATVDLRKIFDNYWKTK
                     QADAALKDRAADIEKDHKTMLDDRKKALDEYQTLLADANNQTLSIEEREKRKKAAEDK
                     FKQIKESEDAIGQYERQARATLDEQRKRMRDSIVEEIRTAVNGKAKLAGYALVFDTTA
                     ESVLNTPVVLYSNNENDMTSAVLAQLNAGGPAEMPKLEDKAADKKSEKKQDKK"
     gene            complement(3711..>5971)
                     /gene="bamA"
                     /locus_tag="VIL39_03580"
     CDS             complement(3711..>5971)
                     /gene="bamA"
                     /locus_tag="VIL39_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY5480535.1"
                     /translation="RAAVDDDVRTLYATGFFYNIQVVADDAGDGLGLVLTYKVQGKPR
                     LTEIKFQGNTKFKNPKLLKKISSKVDDPLDERKLFTDAQDIKKLYQKSGYQRTEVKYL
                     LNIDENAGRGTATFQITESGKVKILEVDFVGANAIPQKKLRRVIKTRKHWMFSWLTTH
                     GVFKDDQFDEDKEKLTEFYREKGYIDFEIKDVQILNPTPRTMIIRITIYEGTQYKIGS
                     VKFTGNELFTTPEIIAGLRVQHQRSRSKAKIGTNGLPMDVGGTFTPGGMTKNIEAMED
                     FYGARGYIDVRAASRNLNVLKIPNTERSTMDLEFQIEEGQKSYIEKIEIRGNTKTRDK
                     VIRRELAVSPGELFDMVRVKLSKQRIENLGYFERVDTQPETIDVPNRRNLVVDVEEKT
                     TGHVSLGAGFSTVDSLVGIAEYNEANFNLAHPFQPPWFRGGGQKLRLRLTIGTERQDY
                     EITFIEPWFLDRKLQLSVDLYYHDYAFLSPNTLYDETRGGGKVGIERALGSDFLRGGI
                     SYKVEDVGIDLTSSAITSGFLRNVPPDILSQTGYHLLSSAGASLAYDTRNSVKLPDKG
                     QRTSLTAQLTGGPLGGSDSFYKLELQHARYIRGFLPGHVLELGGRAGVAQAYGSTEDV
                     PFFERYYLGGLYSLRGFKYRYISPRQPGYNEPVGGDTYWFGSAEYSIPIFEQERGIGV
                     RVAIFYDIGSVGSNPYNFNVSNFSDNWGLGLRLNLPIGPLRLDYGIPIRHDQYSGSSG
                     QFQFGVGWERPF"
BASE COUNT         1148 a         1750 c         1834 g         1239 t
ORIGIN      
        1 ttgccctcga agggcaggtt ggcggtcgcg aggaacttat ccaggagatg gacggcgttg
       61 ttcaggaaat aagcgtctgc ctcgccggcg gagatgtgga tcttgccgcg cagcttgggc
      121 ccgagcgttg gccagttctg ctccagcacg aggcgcaggt cgtatttctt ccactgctcg
      181 gcgaccgcgt gattgatcct gccggtttgc gggtcccata gcgggaccgg gagcccgccg
      241 gcgccgcgcg gcccgaaggc ggcattccat tcgccccact gctcgccgga cagggtgtaa
      301 ctgttgcccc ggcccagcag attctcgacg ccgacttcgc ggcgcatggt cagccgcacg
      361 tcgccgtttg ggtcgcgatc gctcggccgg tcgttgccgt atttgttcac atacgcattg
      421 tcgtcctggt agatgttgac cagttcgagc gcgcggaagt ccacggggtc ggggcacgag
      481 gaccaggtgc cgttgaagaa gtcgggataa aagacctgca gcgcgaggga gacccagccg
      541 cccgtggagt tgccattgag cacgcgcccg cgcgattgcc cgatggcccg gaaccgggcc
      601 tcgacgtgcg ggatgagttc ctgcacgagc gcatcgccga atgggccgtt gttcgccgag
      661 ttgatgtagt aagggtcgcc gttggggccg gcgccgtcca gttgcaggag gatcaggcgc
      721 ggcgtgtcgt cggcctgcca ggtttgccgg aactcggact tttcgcccat caggccgagg
      781 acgctcgtgt agcggccgtt caagccagcg atgcggacca acaacggata gcgccgcgac
      841 gggtcacgct cgtaatcgcg gggcaggacg attcccgcgc gcaggaagat tggccgcccg
      901 tggaactcgc tcagcagctt cgattgaatc ttgacgaact tgatctgcgc cgtttcggca
      961 ggcaactggt ccgcggggat ctgctccgtc agctccagct ttcgggtgcc gtcctgtccg
     1021 gggtcgaggt gaatcttctg cggcttgctg tagaggttgc ccggcgcctc cggcgagcgc
     1081 aggtcggtat tggaatcaaa caaggcctgc acgaagtaat cgcccgcagg cacgcgggcc
     1141 agattcgtga tggggaaagc aaaggtgccc tggccaacca gcgcgactgc gcccggcgca
     1201 aaacccttga ggtcgcacgc caggacttgc ggcgcatcga gaccagtccg gccgagtgta
     1261 agacgcggtt cggggttgtt cgttttggcc aggatgacaa acagccgccc gtctttgggc
     1321 ggtgcggcca ggttcttgtg gatggcgacc tcgaactgcc aggcggctga gttcggtgag
     1381 ctggccggga gagcggccag aggcgcgagg cagagcacga aaccgagcag atggagccga
     1441 agtgtcttca ctttgcgaga tttgcagcct ggctgtcaac ggcgggctgg ctgtccctgc
     1501 tcaatcgtcg gctgaccaat acggtggtgc cgagcagcac cgctgcgcag atcaggtagg
     1561 gcaggggggg cgaccaggac agcagggcgg tggcgaaaac cggccccagg atgcgcgcca
     1621 ggctcccggc accctgggcg accccgatgg tcgcgccctg ttcgttatcg ggggtgaggt
     1681 tcgagagcag gccgaacaag ggcggtcgcg tgagactggt gccgaccgac aaaagcgcga
     1741 gggccataag cagccacacc cagggcaagc catcgtgctg gaagagcacc ttccaggaaa
     1801 gctgcgaggc gcccttgatg aacggaatcc aggcgaagct ggcggcggtc agaacaaggc
     1861 tcaaggcaat cagccggggc tcgcccattc gcttgacgag ccgcccaatg gccccccctt
     1921 gcaccagggc gccgatgaga ccgcaataga cgaacaagta gcccaccgtg ctggctgagg
     1981 tcgcatcgcg tacaaagtcc agatggaagt tggtgccgat cagcaggggc agcgtgctct
     2041 cgaaacagct aaagcagaac gtggcgagaa agaacacaat gaccaggaag cccactttgg
     2101 gttcgcggag tgtgtgcgcc cactggtcga ggtggggccg gggggcagcc tggtcggaat
     2161 cgggcttgtg actctccgtc agaatgaaaa gcgccagcag gaaattcgcc gcgcacagcg
     2221 ccgctgcgca ccagccaggg ccggtgtccc cccagtgtct cagactttcg gccccgataa
     2281 acggcccgag gatgaacccc agcccgaagg ccataccgat aaggcccatt ttcttcgagc
     2341 gttccgccgg cggggtgatg tccgcgatgt aagcctgcgc caccgtgatg ttgccgccgc
     2401 aaaccccggc gaacatccgc gacaccacca tcagccacaa ggcggcagtg tggttttcca
     2461 ggcccgtgct gcgcgcgaac agcacgtaag acgctgccgc cccagcggtg ctgatcagca
     2521 ggatgggacg gcggccatgg cggtccgaca acctccccca gatcggcgag aagatgaact
     2581 gcatcgcgga ataggacgca aagataacgc cgatgaccac gccgtgggcg ccgaagtgtt
     2641 cgctgtaaga cgggatcagg ggcacgataa tcccgaaccc gatgaggtca atgaaaacgg
     2701 tcaggaagat gaccaggacg gagggtcttc tcatggacaa ggcgcgcgaa gcgtctcgac
     2761 accccacggc tggctcgcaa atgacatagc gagagtctat tggcggatca gcgccagtca
     2821 agtgagggac gctgattgtc tcattccccg gtttggcctg tcttaaccag ggacaactgg
     2881 acaagctccc gacctgagtc agtattcact ggcgctgctg ctgctgctga gcccctggaa
     2941 acggggccta cttcttgtcc tgcttctttt cgctcttctt gtctgccgct ttgtcctcca
     3001 gcttgggcat ttctgcgggc ccgccagcgt tgagctgggc cagaacggcg ctggtcatgt
     3061 cgttctcgtt gttggaatac agcacgacag gggtattgag gacgctttcg gccgtagtgt
     3121 cgaacaccag cgcgtaaccg gccaatttcg ccttgccatt aacggccgtg cggatctcct
     3181 caacaatact gtcgcgcatg cgcttgcgct gctcgtctaa ggtggccctt gcctgtcgtt
     3241 catactgccc aatggcgtct tccgattctt tgatttgctt gaacttgtcc tcggccgcct
     3301 tcttgcgctt ctccctttcc tcgattgaga gagtctggtt gttggcgtca gcgagcaagg
     3361 tttgatactc gtccagggcc ttcttccgat cgtcgagcat cgtcttgtgg tccttttcga
     3421 tgtcggccgc ccggtctttg agcgcggcgt cggcctgctt ggttttccaa taattgtcga
     3481 agatcttgcg cagatcaacc gtggcaacgc gaccctgagc caaggcggag ctggtcatca
     3541 gggaaatcaa gagcaatgcc ggaaccatct ttcgtaacaa acttttcatt attgagctaa
     3601 ccaatcagaa ttttaccaag tcgccaacct aaaactgcac gcagtcggtt gcgctttctc
     3661 gaggtagaga caagacatag ccacaaacgt tcaggatttt gaagtccggg ctagaacggg
     3721 cgttcccaac ccacaccaaa ctggaactga ccagagctgc cgctgtattg gtcatggcgg
     3781 atggggatgc cgtagtcgag ccgcagtgga ccgatgggga gattcaggcg cagaccaagg
     3841 ccccagttgt cgctgaagtt gcttacgttg aagttgtaag gattgctgcc cacgctaccg
     3901 atgtcgtaga agatcgcgac tcgcacgccg atgccgcgtt cctgctcaaa gatcgggatg
     3961 ctgtattccg ccgacccaaa ccaataggta tcgccgccga caggttcgtt atagccaggt
     4021 tggcgcgggc tgatgtagcg gtatttgaag ccgcgcagcg agtacaggcc gcccagataa
     4081 taacgctcaa aaaacggcac gtcctcagtg ctgccgtagg cctgggctac gcccgcgcgc
     4141 ccgcccagtt ccaatacgtg gccgggaagg aacccgcgga tgtaacgcgc gtgttggagt
     4201 tcgagcttgt aaaagctgtc acttccaccc agcggaccgc ccgtgagctg ggccgtcaat
     4261 gaggtgcgct ggcccttgtc gggcagcttg acgctgttcc gggtatcgta agcgagtgag
     4321 gcccctgcgc tcgacagcag gtggtaaccc gtctgactca aaatgtccgg cgggacgttg
     4381 cgtagaaaac cggaggttat ggcgcttgag gtaaggtcga tgcccacatc ctcgactttg
     4441 tagcttatac ccccccggag aaagtcgctg cccaaagcgc gttcgatacc gaccttcccc
     4501 ccgccacgag tttcgtcata gagggtgttg gggctcagga acgcatagtc atgataatac
     4561 aaatcgacgc tcaattgcag tttgcgatcc aggaaccaag gctcgatgaa agtaatctcg
     4621 taatcctggc gctccgtgcc gatggtcagc cgcaaccgga gtttctggcc gccgcctcgg
     4681 aaccatggcg gctggaaggg atgggccaaa ttgaagttgg cctcgttgta ctctgcgatc
     4741 ccgaccagcg agtccacggt gctgaagccc gcacccagcg agacatggcc ggtagtcttt
     4801 tcttccacgt ccaccacgag gtttctgcga ttcggcacat caatcgtctc cggctgggtg
     4861 tccactcgct cgaaataccc cagattttca atgcgctgtt tgctgagctt gacgcggacc
     4921 atgtcgaaga gttcacctgg agacaccgcc agttcgcgcc ggatgacctt atcccgggtt
     4981 ttcgtattgc cgcggatttc gatcttctcg atgtaggatt tctgtccttc ctcgatctgg
     5041 aattccaggt ccatcgtgct gcgttctgtg ttcgggatct taagcacatt caagttccgt
     5101 gaggcggcac gaacatcgat gtaacctctg gcgccgtaga aatcctccat cgcctcgata
     5161 ttcttggtca tacccccggg cgtgaaggtg ccgcccacgt ccattggcag tccattggtt
     5221 ccgattttgg ctttgctgcg gctgcgttga tgctggacgc gcagcccggc gatgatttcc
     5281 ggcgtggtga acaattcgtt gccggtgaat ttgaccgagc cgatcttgta ctgggtgccc
     5341 tcgtaaatcg tgatgcgaat gatcatggtc cggggcgtcg gattcaggat ctggacatcc
     5401 ttgatctcga aatcaatgta gcctttctcg cgatagaact cagtcagctt ctctttatcc
     5461 tcgtcgaact gatcgtcctt gaagaccccg tgggtggtga gccaggaaaa catccaatgc
     5521 ttccgtgtct tgatgaccct gcggagcttc ttttgcggga tcgcgttcgc gccgacgaag
     5581 tccacctcga ggatcttgac ttttccactc tcggtgatct ggaacgtcgc cgtgcctcgg
     5641 cctgcattct cgtcgatgtt caacaagtat ttgacctccg tgcgctggta gccggacttc
     5701 tgatacagct ttttaatgtc ctgcgcatcc gtgaacagct tccgttcatc cagcgggtcg
     5761 tccactttcg atgaaatctt cttgagcagc ttcgggttct tgaacttcgt attcccctgg
     5821 aacttgattt ctgtcagccg cggcttgcct tggaccttgt aagtgaggac gagcccgagc
     5881 ccatcccccg catcgtcggc caccacctgg atgttataga agaagccagt tgcatagagg
     5941 gtgcgcacgt catcatcgac cgccgcccgg a
//
LOCUS       DATCGS010000178         5899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_709763, whole genome shotgun sequence.
ACCESSION   DATCGS010000178 DATCGS010000000
VERSION     DATCGS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709763"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2102
                     /locus_tag="VIL39_03585"
     CDS             237..2102
                     /locus_tag="VIL39_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5480536.1"
                     /translation="MHLQLIDWVIVLATLLVCFVPALFFGKRAGKNTSEFFASGRSVP
                     WWLAGLSMVATTFSSDTPNWVTEQVRKFGVAGNWQWWAFVLTGVATVFFFARLWRRSG
                     VMTDLEFYELRYSGKAASVVRGFRAVYLGLFFNCFIMGMVTLAACKIANILFGLPPWQ
                     TIIFCGILNVVFASHSGLWGVLVIDMVQFFIKMTAVVAAAYFSLKQVGGLGVLVEKLG
                     HLQVIMPEGGQPVMSAIDGTGQPILNILPNFNMYQLALMIFIMPIAIGWWANWYPGAE
                     PGGGSYIAQRMLASKSEKDSLGGTLFFNLAHYVLRPWPWIITALCSIIIYPDLASIKA
                     AFPHADPNLIGHDSAFPAMLKFLPVGFVGLMVGGLIAANSSTILTHLNWGSSYLVHDL
                     YRRFINPTATEKHYVNTGRLCTVMLYIFAALLSTMMSSAQQAFQLLLSIGAGTGLLYI
                     VRWFWSRVSAWCEIAAMAASLISAAGFPYLEKVGVLPHLGFAKTTILQVGFTTICWLI
                     AAYIAPQTERSKLIAFYKKVHPAGPGWTKVRKEAGVTEAEATLHGDHMGKATLGWVSG
                     CTVIWSSLFAIGNFLYGRMQPALILTGVFVVSGVVLLWVVNHLWDKKNGDASPAV"
     gene            2165..3763
                     /locus_tag="VIL39_03590"
     CDS             2165..3763
                     /locus_tag="VIL39_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY5480537.1"
                     /translation="MTASTFAADTPALIPLPQKMECREGTFKLEPKTPVLTDAAAQET
                     GNYLAERLSKATGYALKVGKSTKAQPGKGTIFLTTKDAKPELGAEGYELTVTADSVVV
                     RAGKSAGLFYGVQSLLQLMPPEVFAAKPVAGTDWKIPCVQIEDQPRFKWRGFMLDVAR
                     HFFTKDEVKEMLDVLALHKVNTLHMHLTDDQGWRIQIKKYPRLTEIGAWRKDAGFGLD
                     PKASTTYGPDGRYGGYYTQDDIREIVAYARGRHITVVPEIEMPGHASAALAAFPELSC
                     NGGPYTPNAEGGVFAGVYCAGKDETFEFLQNVLAEVRQLFPGQYIHIGGDEVPKDNWK
                     KCPRCQARMKQEGLKTEHELQSYFTRRVEKFINAQGRNLIGWSEIREGGLAQNAVVMD
                     WIGGAVEAASAGHDVIMSPTKFCYLDYCQSTNHATEPKAIGGYLPLSQVYSFEPLPAN
                     LDPQYESHILGAQGNLWTEYVPNLKHAQFMAFPRVCALAEVTWSPKASRNWDDFTRRL
                     QTQFQRFDQLGVNYRKGTPDRIGE"
     gene            complement(3970..4851)
                     /locus_tag="VIL39_03595"
     CDS             complement(3970..4851)
                     /locus_tag="VIL39_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5480538.1"
                     /translation="MRHFALFWAAFDPRTQVPAAGLFGPAYRRAPVHIYTSEQIAALL
                     ARAQQLPPQETLWPRTLSTLLGLLACTGLRISEALHLQPEDWEPTQAVLTIRQAKFGQ
                     SRYVPLAPSAASVLKTYLRARVSEALQLRHQDIHWGAPVTVCLHGKGRKERAIPLLKP
                     IAQELKRALAKLPPEPASLVFENRFGQPLTRWGVEKRLRRTVQQAVQHCPSLKGRRVS
                     PHTFRHTTAMGLLQADVDIMVIALILGHESPTTTHHYIELDIQMKERCLRKLASPKTK
                     TARFQPTDQLLKFLDTL"
     gene            complement(4977..5459)
                     /locus_tag="VIL39_03600"
     CDS             complement(4977..5459)
                     /locus_tag="VIL39_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480539.1"
                     /translation="MNTIVSKSTLDPLVEEYHKHLTEVAGLQPSSCRVYTFFVRSFLH
                     AQCKPNAPSWNLRQLDPRVLLDFVLRQSRGCTANRLQILGSALRSFCRFLCVTQRHGQ
                     DLSGAIPPISGHHREALPTYLSRPELRQLLGKFDRRTVVGKRDYAIGWALRSRMRERC
                     "
BASE COUNT         1149 a         1710 c         1864 g         1176 t
ORIGIN      
        1 gcccgatcaa attcgagctt ctcggccagg agcttcaggg cctgttcgag cgtctcgctt
       61 cccagcttct taggcataaa aggtatttaa accatctgaa gtggataata caactacttt
      121 tatacaatcc ccaggtctgg cttggggggc tccatcaggc ttgttttcgt tggcgaaggt
      181 cggtagcgtc ctcggccgtt ggcgcttaag ccgcccatgg tcggaagaat tctggtatgc
      241 acttacagct tattgactgg gtcatcgtct tagccaccct gcttgtttgt tttgttcccg
      301 cgctgttctt cggcaagcgc gcggggaaga acacttcgga gttcttcgct tccggccgct
      361 cggtgccgtg gtggctggcg gggctttcca tggtcgcgac caccttcagc agcgatacgc
      421 cgaactgggt gaccgaacag gtgcgcaagt tcggcgtggc gggcaactgg caatggtggg
      481 ccttcgtgct caccggcgtg gccacggtgt tcttcttcgc ccgcctctgg cggcgctccg
      541 gggtgatgac ggacctggag ttttacgagc tgcgttattc gggcaaggcg gcctccgtcg
      601 tgcgcggctt tcgcgcggtc tatctggggc tattcttcaa ctgcttcatc atgggcatgg
      661 tgaccctggc cgcctgcaag atcgccaaca tcctcttcgg cctgcctcca tggcaaacga
      721 tcatcttttg cggcatcctc aatgtcgtgt tcgcctcgca ctccggcctg tggggcgtgc
      781 tggtgattga tatggtgcag ttcttcatca agatgacggc ggtggtcgcc gcggcgtatt
      841 tttccctgaa gcaagtcggc ggcctgggcg tgctggttga aaagctcggc cacctgcagg
      901 tgataatgcc cgagggcgga cagccggtga tgtcggccat tgacggcacc ggccagccga
      961 tcctcaacat cctgcccaac ttcaacatgt accaactggc gctgatgatt ttcatcatgc
     1021 ccatcgccat cggctggtgg gccaactggt atccgggcgc ggagccgggc ggcggcagct
     1081 acatcgccca acgcatgcta gcctccaagt cggagaagga ttccctcggg ggcaccctct
     1141 tcttcaacct ggctcattat gtgttgcggc cctggccgtg gatcatcacc gcactctgct
     1201 ccatcattat ttaccccgat ctcgcctcca tcaaagccgc ctttccccat gccgatccca
     1261 atctgattgg ccatgacagc gcgtttccgg ccatgctgaa gttccttccc gtgggctttg
     1321 tgggactgat ggtcggcggg ctgattgcgg cgaactcctc gacgatcctg acgcatctca
     1381 attgggggtc ctcctacctg gtgcacgacc tctatcggcg cttcattaac cctactgcca
     1441 cggagaagca ctatgtgaat acggggcgcc tctgcaccgt gatgctttac atcttcgcgg
     1501 cgttgttgag cacgatgatg agttcggcgc agcaggcgtt ccagctcctg ctttccatcg
     1561 gcgccggcac gggcttgctt tacatcgtgc gctggttctg gtcgcgcgtc tcggcgtggt
     1621 gcgaaattgc ggccatggcg gcgtcgctga tcagcgcggc cgggttcccg tacctcgaga
     1681 aggtgggcgt gctgccccac ctgggctttg cgaaaaccac catcctgcag gtgggtttca
     1741 cgacgatctg ctggctcatt gcggcgtata ttgcgccgca aaccgagcgc tcgaagctga
     1801 ttgcgttcta caagaaggtg catcccgccg ggccgggctg gacgaaagtg aggaaggaag
     1861 ccggcgtgac ggaggccgaa gcgacgctgc acggcgacca catgggcaag gccacgctgg
     1921 gctgggtttc cggctgcaca gtgatctggt cctcgttgtt cgccattggg aactttctct
     1981 acggccgcat gcaaccggcg ctgatcctca ccggcgtatt tgtggtgagt ggcgtggtcc
     2041 tgctctgggt ggtgaaccac ctctgggata aaaagaacgg ggacgccagc cctgccgtgt
     2101 agaacaagaa tccaaaccag gcaacaacat gagataccaa actgctgcaa tcctcctcgc
     2161 tctgatgacc gcaagcactt ttgccgcgga caccccggcg ctgattccgc tcccccaaaa
     2221 gatggagtgc cgggaaggga cgttcaaact cgaaccgaaa acgcccgtcc tgacggacgc
     2281 ggcggcgcaa gagaccggaa actacctggc tgagcgcctg agcaaagcta cgggttatgc
     2341 cctcaaggtc ggcaagagca ccaaggcaca gccgggcaaa gggacgatct tcttgaccac
     2401 caaggacgcc aaaccggaac tgggcgccga aggctacgag ctgaccgtga ccgcggattc
     2461 tgtggtcgtg cgggcgggca agtctgccgg cctgttctac ggggtgcagt cgctgctgca
     2521 actgatgccg ccggaggtgt ttgcggcgaa gccggtggcg ggcacagatt ggaagattcc
     2581 atgcgttcag atcgaggacc agccgcggtt caagtggcgg ggattcatgc tcgatgtggc
     2641 ccgccatttc ttcaccaagg acgaggtcaa agaaatgctg gatgtgctgg ccctgcacaa
     2701 ggtgaacaca ctccacatgc acctgacgga cgaccagggc tggcggattc aaatcaagaa
     2761 gtatccccgc ctgaccgaga tcggggcgtg gcggaaggat gcggggttcg ggctggaccc
     2821 gaaggccagc acgacctatg gcccggacgg acgatatggc gggtattaca cgcaggacga
     2881 catccgcgag atcgtggcct atgcccgcgg gcggcacatc accgtcgtgc cggagatcga
     2941 aatgcccggg catgcgagcg cggcgctcgc ggcgtttccc gagctgagct gcaacggcgg
     3001 gccctacacg ccgaacgcgg aaggaggagt cttcgcgggg gtctattgcg cgggcaaaga
     3061 tgagaccttc gagttcctgc aaaatgtcct ggcggaggtc cgccagctct tccccggcca
     3121 atacatccac atcggcgggg acgaggtgcc caaggacaat tggaagaaat gtccccgctg
     3181 ccaggcccgc atgaaacagg aaggcttgaa gaccgagcat gagctgcaga gctacttcac
     3241 ccgccgcgtc gagaagttca tcaatgcgca gggacgcaac ctgatcggct ggagtgaaat
     3301 ccgcgaaggc ggcctggcgc agaatgcggt cgtgatggat tggattggcg gggcggtcga
     3361 ggccgccagc gcggggcacg atgtcatcat gtcgcccacc aagttttgct acctggacta
     3421 ctgtcagtcc accaaccacg ccacggaacc caaggccatc ggcggctact tgccgttgag
     3481 ccaggtgtac tcgtttgagc cgctccccgc gaacctggac ccgcaatacg agtcgcacat
     3541 cctgggcgcg cagggcaacc tgtggacgga gtatgtcccc aacctcaagc atgcgcagtt
     3601 catggccttc ccgcgcgtgt gcgcgctggc agaggtcacc tggtcgccca aagcctcgcg
     3661 caactgggat gatttcacac ggcgcctgca gacgcagttt cagcgcttcg atcagcttgg
     3721 cgtcaactac cgcaaaggca ctcccgatcg catcggcgag tgagcggcct gggccgaggg
     3781 gtcgtagcgc cgacttcggg gtccaatgcc gctaggattt gaccctcctc atcccggagg
     3841 gatggtcgag aatagccctt atggggaact ccccataagg gctattatgg ggacatgaat
     3901 attatgggga catacggaag aaagtggggg gttttcccgg gtattttggc taggcatgtc
     3961 cccataatat cagagggtgt ccaggaactt taagagctgg tctgtgggct ggaatcgagc
     4021 cgttttggtt ttaggcgatg cgagcttgcg cagacatcgc tccttcatct ggatgtccag
     4081 ttcgatataa tggtgggtgg tggtgggcga ctcgtggccc aagatcagcg caatgaccat
     4141 gatgtccacg tcggcctgca gcaaacccat ggcggtggtg tggcggaagg tgtgaggcga
     4201 aaccctccga cccttgagcg agggacaatg ctgcacggcc tgctggacgg ttcggcgcaa
     4261 acgtttttcc actccccagc gggtcagcgg ctggccaaag cggttttcaa aaaccaggct
     4321 tgcaggctcg ggtggaagtt tcgccagtgc gcgtttcaac tcttgggcga tgggtttgag
     4381 cagggggata gcccgctctt tgcgtccctt gccatgcaag cagacggtga cgggcgctcc
     4441 ccaatggata tcctggtgtc gcagttgcaa ggcttccgag acgcgcgccc gcaaataggt
     4501 cttcaagacg ctggcggcgc tgggggccag cggcacgtag cgggattggc cgaacttcgc
     4561 ctggcgaatc gtgagcacgg cttgcgtcgg ctcccaatcc tccggttgca ggtggagggc
     4621 ttcggagata cgcaatcccg tgcaggccag caaccccagc agcgtgctca aggtgcgtgg
     4681 ccacagggtc tcttgcggag gcagttgttg cgctcgggcc aacagggccg cgatctgctc
     4741 ggaggtgtag atatgtacgg gggctcggcg ataagccggc ccaaacagac ccgctgcggg
     4801 gacttgggtg cgcgggtcaa aggctgccca aaagagcgcg aagtgtcgca cggcattcaa
     4861 gcgccggacc cggcgcggcg agtgtgtcgg cggaggcacc tgcgcccaac tcaaggccaa
     4921 cgccgaggtc aaggggcccc gatgctgaag tttccgggca tgctcgacga gatttctcag
     4981 caacgctccc tcatccttga gcgcaaagcc caaccgatgg cgtaatcgcg tttgcccact
     5041 accgtgcgac ggtcaaactt tcccagtaat tgcctgagtt ctggacgact caaataggtt
     5101 ggcagcgctt cccgatgatg gcccgagatc gggggaatcg ccccgctaag atcctggccg
     5161 tggcgctggg tcacgcacaa aaaacggcaa aaggagcgta aggccgaccc cagaatctgc
     5221 agccggttgg cggtgcaacc gcggctttgc cgcagcacga aatccaacag aactcttggg
     5281 tccagttggc gtaggttcca tgacggcgcg ttgggcttgc actgcgcgtg caaaaacgag
     5341 cgaacgaaga aggtgtaaac ccgacagcta ctcggctgga gtcccgccac ttcggttaga
     5401 tgtttatgat attcttcgac cagagggtcg agggttgatt ttgagacgat agtattcatc
     5461 gccccagtgt cagaaccttg ttgacgcccc tcaactatta tggggacatg cccagccaaa
     5521 atacccggaa aaccccccgc tttcttccgt atgtccccat aatattcatg tccccataat
     5581 agcccggcgt ttcaacgctg gggcagcggc ggcggcaagg cggggttagc gctgtcaaag
     5641 cccagtaggg gcgtggcggc accgctgtgg cgccgcgaag gtcgccacca ggaaaaacag
     5701 ggaaaacacc aagagaatac gaagagaata cgaagagaat acgaagagaa tacgaaggga
     5761 acacgaagag aatacgagga cacctcccgt gctactggcg tgcagatggc ctgcgagtgg
     5821 ctggtgggtg gcttgcgggt ggccttgggg tgactttgcc tggggctgtg cgcttcggtt
     5881 ctcagccagg gcgggcttg
//
LOCUS       DATCGS010000179         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_462677, whole genome shotgun sequence.
ACCESSION   DATCGS010000179 DATCGS010000000
VERSION     DATCGS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462677"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VIL39_03605"
     CDS             <1..811
                     /locus_tag="VIL39_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEY5480540.1"
                     /translation="PTDHDLPVLVARDESGKVRALLMSYACHCTTLGATPNHICGDWA
                     GYAREYLEGDYPGAVALVALGCGGDANPQPRGNLDFAKQHGNAVGAAVNELLRQPLTP
                     LRQKLACRVKLITLPYDTLPTREEWTARAESTNHWIAYHAKLNLSRLDRSERLPTELP
                     YLVQTWNFGTELAMVFLPGEVVVDYSLRLKKEYDSARLWVNAYANDVPCYIPSKRVWR
                     EGGYEGGGAMIYYDRPARLVEGTEDLIVAAVHGLLPRSYSHPRRHAGRSLE"
     gene            922..2004
                     /locus_tag="VIL39_03610"
     CDS             922..2004
                     /locus_tag="VIL39_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEY5480541.1"
                     /translation="MNPGKSLLSTTVLNFAVISALQLAGQPACFGQPDPNWLDHDCNR
                     PQPPVVAPATASTQDQAGKPPSDATVLFDGKDLSHWVSMDGSPTKWITRDGYMECVKG
                     SGYVRTRQNFGDCQLHVEWATPMPGQGEGQGRGNSGVFLGLDRYEIQVLDSYENKTYA
                     DGSAGAIYGQYPPLANICRPPGQWQTYDIVYTAPRFDAEGKLLSPVRLTVLQNGVLIQ
                     NNVQLTGPTSWLERAPYQAHPEKQPISLQDHGNPVRFRNIWVRELGKLDKKEVAPRPA
                     GLTLPTSLLDSYAGKYDSIEITREGSQLAARVGGVKFLMFAESPTKFFAKTTDVQIEF
                     PGGSQGKPDRLTWSVGEGANEAKRDK"
     gene            2144..>2943
                     /gene="recA"
                     /locus_tag="VIL39_03615"
     CDS             2144..>2943
                     /gene="recA"
                     /locus_tag="VIL39_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY5480542.1"
                     /translation="MPPKTAEKTVEKPAAADLKAAEAAKASATRARDLDAAISSITKA
                     YGDGSIMRLGDARSLRQIEVIPTGALAMDLALGVGGIPRGRVVEIFGPESSGKTTLML
                     HVIANAQKTGGLAAFIDAEHALDPGYAKKLGVNLDDLLVSQPDSGEEALTICETLARS
                     NALDVIVIDSVAALVPKAELEGEMGMATMGMQARLMSQALRKLTAILAKAKTTCVFTN
                     QMREKVGVMFGNPETTPGGKALKFYASVRIDIRRKETLKDAAGNAVGN"
BASE COUNT          585 a          908 c          930 g          520 t
ORIGIN      
        1 tccaaccgac cacgacctgc ctgtgctcgt ggcgcgggat gagtccggaa aggtccgtgc
       61 gttgctgatg agctacgcct gtcactgcac cactctgggc gccacgccca accacatctg
      121 cggcgactgg gccggttacg cacgagaata ccttgagggc gattatccgg gggcggtggc
      181 gctcgtggcg ctgggctgcg gcggggatgc caatccgcag ccgcgcggca acctcgactt
      241 cgccaaacag cacggcaatg cggtcggcgc tgccgtcaat gaactcttgc ggcaaccgct
      301 gacgccgttg cgccaaaagc tggcctgccg ggtcaaactc atcactttgc cgtacgacac
      361 actgcccacg cgcgaggagt ggacggcgcg ggcggaatcg accaatcact ggattgccta
      421 ccacgcgaaa ctgaatctaa gccggctcga ccgcagtgag aggctgccga ctgaactgcc
      481 gtatctggtc cagacgtgga atttcggaac cgaactggcg atggtctttc tgccgggcga
      541 agtggtggtg gattattcgc tgcgcctgaa aaaggaatat gattcagcgc gcctctgggt
      601 gaacgcctac gcgaatgacg tgccctgtta cattccctcg aagcgtgttt ggcgtgaggg
      661 cggctacgag ggtggcggag caatgatcta ctacgaccgc ccggcccggc tggtcgaggg
      721 aacggaggat ctgattgtcg cagcggtgca cgggttgttg ccgcggtctt actcgcaccc
      781 caggcgccac gccggtcgca gcctcgaata atgtgagcct cgcacccagc ctgcacgcgc
      841 gcgcaaagaa gtcttttctc gaacgcgcaa agcgtttagg ctgcttgccc agatgcagtt
      901 taactaccaa tgtccactct tatgaatcct ggaaaatctt tgctatccac cactgttctg
      961 aacttcgccg tcatcagcgc gctgcaattg gcgggtcaac cggcctgctt tgggcagcct
     1021 gacccgaact ggctcgacca cgactgcaac cggccgcagc cgccggtggt ggcgccggct
     1081 accgccagca cgcaagacca ggccggcaaa cctccgtccg atgccactgt cctgttcgac
     1141 ggcaaggacc tctcacactg ggtcagcatg gacggcagcc cgaccaaatg gattacccgg
     1201 gatggctaca tggaatgcgt caaaggcagc ggctacgtcc gcacgcgcca gaatttcggg
     1261 gattgccagc ttcatgtcga gtgggcgacg ccgatgccgg gccagggcga gggccagggg
     1321 cgtggcaaca gcggcgtctt cctcggcctg gaccgctacg aaatacaggt gctggactcg
     1381 tatgagaaca aaacctacgc cgacggcagc gcgggcgcca tttacggcca atacccgccg
     1441 ctggccaaca tctgccgccc acccggccaa tggcagactt atgacattgt ctataccgcc
     1501 ccgcggtttg acgccgaagg caagctgctt tcccccgttc gcctgaccgt gttgcagaac
     1561 ggcgtgctca tccagaacaa cgtccagctt accggcccga ccagttggct ggagcgggcg
     1621 ccttatcagg cgcatccgga gaaacagccc atctcgctgc aagaccacgg caacccggtc
     1681 cgcttccgca atatctgggt ccgcgagctc ggcaagctgg acaagaaaga agtcgcgccc
     1741 cggcccgccg ggcttactct cccgacctca ctgctcgaca gttatgccgg caagtatgat
     1801 tccatcgaga tcacgcgcga aggcagccaa ctggcggcgc gggtgggcgg ggtgaagttc
     1861 ctgatgttcg ccgagtcgcc gactaagttc ttcgcgaaga cgaccgatgt gcagatcgag
     1921 tttcccggcg gcagccaggg caagccggac cggctgactt ggtcggtcgg cgaaggcgcc
     1981 aacgaggcga agagagacaa gtagcttccg gtgtgaaccg agtttgcggg tgtaggatgc
     2041 gttgcccgct ccgcaccccg aggtggtgtt tgactgtcca cttccggctc ggtagcattg
     2101 cgcgtcgtta ttaactaagg ttccaaagca cacgaatacg aatatgcctc ccaagactgc
     2161 tgaaaagact gttgagaagc cggcggctgc cgatctgaaa gcggctgaag ccgccaaagc
     2221 gtccgcgacg cgcgcgcggg atctcgatgc cgccatttcc tccattacca aggcctacgg
     2281 cgacgggagt atcatgcgcc tgggtgacgc ccgctcgctc cggcaaatcg aggtcatccc
     2341 gacgggcgcg ctggcgatgg atctggcgct gggggtgggc ggaatccccc ggggccgcgt
     2401 ggttgagatc ttcggcccgg aatcctcggg caagacgacg ttgatgctgc atgtgatcgc
     2461 caatgcccag aagaccggcg ggctggccgc gttcattgat gccgagcatg cgcttgaccc
     2521 gggctacgcc aagaagctgg gagtgaacct ggatgacctg ctggtttcgc agccggacag
     2581 cggcgaggaa gcgctgacga tttgcgagac gctcgcccgt tcgaatgcac tcgatgtgat
     2641 tgtcattgac tccgtggccg cgctggtgcc caaggccgag ctggaggggg agatgggcat
     2701 ggcaaccatg ggcatgcaag cgcggctgat gagccaggcg ctgcggaagc tcacggccat
     2761 ccttgccaag gccaaaacta catgtgtgtt caccaatcag atgcgcgaga aggtcggcgt
     2821 gatgtttggc aacccggaaa ccacgccggg cggcaaggcc ttgaagtttt atgccagcgt
     2881 ccgcatagac atccggcgca aggaaacgct caaagacgcc gccggcaacg cggtcggcaa
     2941 tca
//
LOCUS       DATCGS010000180         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_165839, whole genome shotgun sequence.
ACCESSION   DATCGS010000180 DATCGS010000000
VERSION     DATCGS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165839"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VIL39_03620"
     CDS             complement(<1..247)
                     /locus_tag="VIL39_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480543.1"
                     /translation="MNKSTTNPLSSSPGSMDRRRFLTATAMVSAITLLPRHVLGGAGQ
                     TPPSEKVNIAGIGVGGMGGANLQALKSQNIVALCDVDH"
     gene            complement(250..3075)
                     /locus_tag="VIL39_03625"
     CDS             complement(250..3075)
                     /locus_tag="VIL39_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480544.1"
                     /translation="MKPTRIVLAAPQMKFRTASFLLCAALSISIQAWPAERWSAVISP
                     DNSLEFSFVKDQAPVARLGLGGWGPNWQWVGLSPHPGAPREGLLTVVPFVVDQARGEV
                     IEIGFGAAKTGLQQVAFSYRLSAAKDVPVTMLIASLSINKAFGRGQLRLTHADGQKSE
                     RPLPFVRGTVLATSSLVLHFEKGGDLTLALDPPCPIAFDGDMRIILASEMFKAGSRTN
                     TLTVSFPSEIAFVAGAGELAPFIRTLAGPDWFAFAPTEDAGPSVISMNEWLDKPAGTH
                     GGVRMAGDHFEFEDKTPVKFWGVNLSYGGGCAPEKKTAEFTAARFAKYGVNGVRLHKF
                     SYPKDQMGIGELKDATQMTPEGLDRLDYFAAQLKSNGVYFGWSHTFGFHVCPGNRSRL
                     LAYDEIERNLKGNTYGFINFAEDVQDLMIEMVVNLLQHRNPYTGRTYAQEPALSFIEL
                     QNEDDIFFYTSEKAFASCPTYRKHFTARFCDWLLARYGSESKLQQAWSGALKPGEALA
                     AKNIEPQTNPWFFSEAHLPGQTGGARQRLLDSAAWLHEMQNKFYSRFVKAIRDAGYQG
                     PLCGSPWQAPAMLPHYYNLQSDALVGYIDRHNYFGGGLFDSMLAQPGSGYFSSGLQQA
                     ANRPFGLSEWIHVYPSLYSAEGPAIVAAYGMGLQGWDASYEFQSQAAPRSFNDRAGWP
                     PWGVWEADVPTQLGQYPALTRMLYRGDVLESQIISTRRVSREEVAAGRFGFSDTMAQR
                     GDIKSFGGSVPPEALAAGRVVVEFTDQPLPSSFPDLAKYRTATAIHSSTGQLVWDTAG
                     QGFFTVNTPGTKAVVGFAEGKELRLGEVTIQLACPYASLFLTALDKNVTLAEAKSALL
                     CAVARNSNSGFKYFTLDSRTLDNGHGPILLEPVRATLTFSRRSVATVNVLDHDGRRTG
                     KSLPVTDGRFSIDGARDKTLYYEVQFR"
     gene            complement(3095..3229)
                     /locus_tag="VIL39_03630"
     CDS             complement(3095..3229)
                     /locus_tag="VIL39_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480545.1"
                     /translation="MTNSFVPSLDLVVTRQTGGSGAWPFEEYLRQACAAISQETREAK
                     "
BASE COUNT          717 a         1020 c         1124 g          676 t
ORIGIN      
        1 cgtgatccac gtcacagagt gccacaatgt tctgcgactt aagcgcctgc aagttcgctc
       61 cgcccatccc acccacgcca atgccggcga tgttaacttt ctcgctcggc ggagtctggc
      121 ctgctccgcc cagcacgtgc ctggggagaa gggtgatcgc cgaaaccatc gcagtcgccg
      181 tcaaaaagcg acggcgatcc atgctgccgg gcgaagagga aagagggttt gtggttgatt
      241 tgttcatagt cacctgaatt gcacctcgta atagagagtc ttgtcgcgtg ccccgtcaat
      301 gctgaaacga ccgtcggtaa cgggcagcga tttgccggtt cggcggccgt cgtgatccaa
      361 cacgttcact gtggcaacac ttcggcggga gaaggtcagt gttgctctta ccggttcaag
      421 caggatcggg ccgtgtccgt tgtccagcgt tcgggagtcg agggtgaagt acttgaaccc
      481 gctgttggag ttccgtgcca cggcgcagag gagcgcgctc ttggcctcgg ccagcgtgac
      541 atttttgtcc agggccgtca gaaagagaga agcgtagggg caggcgagtt ggatggtgac
      601 ctcgccgagt cggagttcct tgccttcggc gaaacccacg accgccttgg tgccaggcgt
      661 gtttactgtg aagaagccct ggccggcagt gtcccagacg agttgcccgg tgctggagtg
      721 tatcgcggtg gcggttcgat acttcgcgag atccgggaaa cttgagggca gcggctggtc
      781 ggtgaattca accacgactc gtccagcggc caaagcttcg ggaggaacgc tgccgccaaa
      841 agacttgatg tcccctcgct gcgccatagt gtcagagaaa ccgaaccggc cggcggcaac
      901 ctcttcccga ctgacacggc gtgtggagat gatctgactt tcgagaacat caccgcggta
      961 gagcatgcgg gtcagcgccg gatactggcc gagttgggtc ggcacgtcgg cttcccatac
     1021 tccccagggc ggccagccgg cgcgatcgtt gaaggagcgg ggtgcggcct gcgactggaa
     1081 ttcatagctg gcatcccagc cctgcaatcc catcccgtaa gcggcgacga tggcggggcc
     1141 ctctgcgctg taaagggaag gatagacgtg aatccattcg ctcagcccga aaggccggtt
     1201 ggccgcctgc tgcaggccgc tcgagaaata gccgctgccg ggctgggcca gcatggaatc
     1261 aaacagccct ccgccaaagt aattatgccg atcaatataa cccacgagcg cgtctgactg
     1321 aagattgtaa taatgcggga gcatggccgg ggcttgccag ggcgagccgc agagcgggcc
     1381 ctggtagccc gcgtcgcgaa tggcctttac gaaccggctg tagaatttgt tctgcatctc
     1441 gtgcagccag gccgccgaat ccagcaatcg ctggcgcgcg ccgccggtct gaccgggaag
     1501 atgggcctcg ctgaagaacc aggggtttgt ctggggctcg atgttcttgg cggcaagggc
     1561 ttcgcccggt ttgagcgcac cggaccaggc ctgctggagc ttcgactcag agccatacct
     1621 ggccaggagc cagtcgcaga accgggcagt gaagtgcttg cgataggtcg ggcaggaggc
     1681 gaaggccttc tccgaagtgt agaagaagat gtcgtcctcg ttctgcagtt cgatgaagga
     1741 gagcgccggc tcctgggcat aggtgcgacc ggtgtaggga ttgcggtgct gcagcaggtt
     1801 gaccaccatt tcgatcatca ggtcctggac atcctcggcg aagttgatga acccataggt
     1861 gttgcctttc aggttgcgct cgatttcgtc gtaggccagc agtcgcgagc ggtttccggg
     1921 gcacacatgg aagccaaagg tgtgcgacca gccgaaataa acgccgttgc tcttgagctg
     1981 cgccgcgaag taatcgagcc ggtccaggcc ctcgggcgtc atttgggtag cgtccttaag
     2041 ctcgccgatg cccatctggt cctttgggta agagaacttg tgcagccgca caccattgac
     2101 cccatacttc gcaaagcggg cggcggtgaa ctcggcggtc ttcttctccg gcgcacaacc
     2161 gccgccatag gagaggttca caccccagaa cttcaccggg gtcttgtcct cgaactcgaa
     2221 gtgatcgccg gccatgcgca cgcccccgtg agttccggca ggcttgtcca gccactcgtt
     2281 catgctgatg acactcgggc cggcatcttc cgtgggcgca aaggcaaacc aatccgggcc
     2341 ggccaaggtc cggataaacg gagccaattc ccctgccccg gccacaaatg cgatttcgga
     2401 agggaaactg accgtgagcg tgttggtgcg agagccagcc ttgaacattt cagaggccag
     2461 aatgattcgc atatcgccat cgaacgcgat ggggcatggc ggatccaagg caagcgtgag
     2521 gtcgcctccc ttctcaaaat ggagtaccag gctggaggtc gccagcaccg tcccccggac
     2581 gaaggggagt ggccgctcac tcttctgccc gtcggcatgg gttagcctca actgcccccg
     2641 tccaaaagcc ttgtttatgc taaggctggc gatgagcatg gtgaccggaa catctttggc
     2701 ggcgctcaac cggtagctga acgccacctg ctgaagccct gttttcgccg ccccgaaccc
     2761 gatctcgatg acctcgcccc tggcttggtc aactacgaat ggaacaacgg tgagaagtcc
     2821 ctcgcgaggg gctcccggat gcggggaaag tccaacccac tgccagttcg gtccccatcc
     2881 gcccaaaccg agccgggcca cgggcgcctg gtccttcacg aagctaaact cgagcgaatt
     2941 atccggtgag atgacagccg accaacgctc cgccggccaa gcttgaatcg aaatggacag
     3001 cgccgcgcag aggaggaagg aagccgtgcg gaatttcatt tgtggagcgg ctaggacgat
     3061 gcgggtgggt ttcatggctg cttgatcata agattcattt ggcttcacga gtttcctgag
     3121 agattgccgc gcaggcctgg cgcaaatatt cttcgaaggg ccaggcgccg ctgccgccag
     3181 tctggcgcgt aacgaccagg tcgaggctgg gcacaaagct gttggtcatg agtagcggaa
     3241 gggacagtgg aaagatcata cggcgttgtc gaaccaaaat ggctgggtca ccgctccggg
     3301 ttgttcagtg gttagggtcg ctaatgggcg gaaggaccac cagcggggga gcattggcgt
     3361 tccgggtaat gcgatcctag ggaacgtaat cggtttacgg atcgaagacc cgcttaaacc
     3421 tcaccgagcc tgcgaacggt tggaacgatg caagaccctc cgcggatgtc ggactcccgg
     3481 atgagaaacg aaagatggcg cccgccccga tttcaggatt gaatgggccc gatgcat
//
LOCUS       DATCGS010000181         3883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_709907, whole genome shotgun sequence.
ACCESSION   DATCGS010000181 DATCGS010000000
VERSION     DATCGS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709907"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIL39_03635"
     CDS             <1..251
                     /locus_tag="VIL39_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480546.1"
                     /translation="ECGHWYARAMSSYALLFGLSGARYDAVEKVLYLEPGIRGDFRSF
                     FSTATGYGTVGVRKGKPFYEAKVGTLEIREVRCVMRDA"
     gene            complement(265..2478)
                     /locus_tag="VIL39_03640"
     CDS             complement(265..2478)
                     /locus_tag="VIL39_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5480547.1"
                     /translation="MGAKQNWIMPLAAALVVACVGWWADSELSQVMREELSDDLQTML
                     HADVTALEIWMVNQKRIAVVLAEEPRLRNLAVELLNKGGAKTQDPMAVAGLARQLMIG
                     DRLQERLNTLGYTVAQLVNTNCEVVLDSGRVRSRMGTQVAEDLQPRFDELFASGEPII
                     ITPFTMQAPESPGLPPGGRGPGRSRGPRRPGLGPRFGGNASTNAATAPRSLTVMQVAA
                     PLKDTNGVTRGALALIINPDAEFSRILSVARSDESGETFAFDPDGVMLSKSRFEEQVK
                     ELGLMRPRPGGSSALTLHLTDPGGNLTRGFNPGNTNSTRPLIEMVRRAVDGSPGVEVE
                     PFRDYRGVPVIGAWMWLPHYGFGVGTKIDAREAYRTLRLVRMVFVILFLLLVLASLII
                     LLYSFRQVVWRRRLTEAELKARQLGQYQLMEKIGEGGMGVVYKAHHALLRRETALKLL
                     MPDKADPVSIQRFEREVRLTCRLMHPNTIQIFDYGHTPEGIFYYAMEYLDGLNLAEFV
                     EAYGPQPEGRVVNLLIQICESLAEAHACGLVHRDIKPANVFVTDRGGVPDMVKVLDFG
                     LVRPVASADNTALDAADTQILAGTPNYMSPEAVESGASTDARSDLYSVGAVGYYLLTG
                     QGVFDGESIAEICRKRREEQPVPPSVRAGRPICPHLEGLILHCLERDPKDRPQSAHEL
                     IALLSASPRIADWNVEQRAAWWVAHREAINRARAAEAEPIETAKAVNIEIEDRAL"
     gene            complement(2487..3275)
                     /locus_tag="VIL39_03645"
     CDS             complement(2487..3275)
                     /locus_tag="VIL39_03645"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY5480548.1"
                     /translation="MTLSNQPLVIAGRTFRSRLILGTGKFSAPEALRDALEASGAEMV
                     TVALRRADLSGKKDPFANILEFIDPKRFLLLPNTSGAMNAEEAVRLARLAVAAGLPKW
                     VKLEIHPDPRYLLPDPIETLKAAETLVKEGFTVLPYINADPVLARRLQDVGTATVMPL
                     GSPIGSNRGIQTRDLIRIIIEQAVVPVVVDAGLGAPSHASEAMEMGADAVLINTAIAV
                     ASDPNRMATAFKMAVEAGRAAFESGLAAPQDAASPTSPLTAFLD"
     gene            complement(3526..3735)
                     /gene="thiS"
                     /locus_tag="VIL39_03650"
     CDS             complement(3526..3735)
                     /gene="thiS"
                     /locus_tag="VIL39_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY5480549.1"
                     /translation="MQNQIIIANAKEVPASLPCSIEAFLVAQNLLPRSVVVEHNGEAV
                     APSEFSHLQLHAGDRLEIVKIVAGG"
     gene            complement(3757..>3883)
                     /locus_tag="VIL39_03655"
     CDS             complement(3757..>3883)
                     /locus_tag="VIL39_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEY5480550.1"
                     /translation="PANATSQFQIADDRSPREVADLIRQLGYEPVWKDWDMALTS"
BASE COUNT          681 a         1216 c         1257 g          729 t
ORIGIN      
        1 acgaatgcgg ccactggtat gcgcgggcca tgtcttcgta tgcgctgctg ttcgggctta
       61 gcggcgcgcg ctatgacgcc gtggaaaagg tcctttacct cgaacccggc atacggggcg
      121 acttccgcag cttcttctcc actgcgaccg gctacggcac ggtgggcgtg cgcaagggca
      181 agccattcta cgaggcgaag gtgggaacgt tggagatacg ggaggttagg tgcgtgatgc
      241 gtgatgcgtg acgcgtgata cgggttacag cgcgcggtct tcgatttcga tatttacggc
      301 cttggcggtc tcgattggct ccgcctcggc ggcccgggca cggttgatgg cttcacgatg
      361 ggccacccac caggcggccc gttgctcgac gttccaatcg gcgatgcgcg ggctggcaga
      421 caacagcgcg atcagttcat gggcggattg cgggcggtcc ttcgggtcgc gctccaggca
      481 atgcaagatc aagccttcga gatgtgggca aatcgggcgg ccggctctga cgcttggcgg
      541 cacgggctgt tcctccctgc gcttccggca gatttctgcg atcgactcgc cgtcaaagac
      601 gccttgcccg gtcaagaggt agtagcccac cgcgccgacc gagtagagat cgctgcgggc
      661 gtcggtgctg gccccgcttt caactgcttc cggcgacatg tagttcgggg tgccggcaag
      721 gatttgggtg tctgcggcgt cgagcgcagt attgtcggcg ctggcaactg ggcgcacgag
      781 gccgaagtcg agcaccttca ccatgtccgg cacgccgccg cgatcggtga cgaagacatt
      841 ggccggtttg atgtcccggt gaacgagccc acaggcatgg gcttccgcca gggactcgca
      901 aatctggatg agcaagttga ccacgcgccc ctcgggctgc gggccgtagg cttcgacgaa
      961 ctcagccagg ttcagcccgt cgagatactc catcgcataa tagaagatgc cctcgggcgt
     1021 gtgaccgtaa tcgaagatct ggatcgtgtt agggtgcatc agtcggcagg tgaggcggac
     1081 ctcgcgttcg aagcgctgga tcgatactgg atcggccttg tcgggcatca gcagcttgag
     1141 cgcggtctcg cggcggagca gcgcgtggtg ggccttatag acgacgccca tgccgccttc
     1201 gccgattttc tccatgagct ggtattggcc gagctgtcgg gctttgagct ctgcctcggt
     1261 cagccgccga cgccatacca cctgccgaaa ggaatagagc aggatgatga gcgaggccag
     1321 aaccagcagc aggaagagaa tgacaaacac catccgcacc aggcgcagcg tgcgatacgc
     1381 ttcgcgcgcg tcaatcttgg tgcccacgcc gaaaccatag tgcggcagcc acatccaggc
     1441 tccaatgacg ggcacgccgc gataatcgcg aaacggctcc acctcgaccc cggggctgcc
     1501 atccacggcc cgccggacca tctcgatcaa gggccgggtc gagttggtgt tgccgggatt
     1561 gaatccccgg gtcaggttgc ctccaggatc ggtcagatgc agggtcagcg ctgagctgcc
     1621 gccgggccgt ggtcgcatta gccccaattc cttgacctgc tcctcgaacc ggctcttcga
     1681 gagcataact ccatccgggt caaaggcgaa cgtttcaccg gattcgtctg aacgcgcgac
     1741 cgacaggatg cgggagaact ccgcgtcggg gttgatgatc agcgccagtg cgccgcgcgt
     1801 cacgccgttc gtgtctttca ggggcgcggc cacctgcatg acggtcagcg agcgcggggc
     1861 agtggcggca ttggtggacg cattgccccc gaaccgcggt cccaaccccg gccgtctggg
     1921 tcccctcgac cgccccgggc cgcggccacc tggcggcaag ccagggctct ccggcgcttg
     1981 catcgtgaag ggagtgatga tgatcggttc tccggacgca aacagctcgt cgaaccgtgg
     2041 ctgcaagtct tcggccacct gggtgcccat gcggctgcgc acgcgcccgg aatccagcac
     2101 cacctcgcag ttggtgttga ccaactgggc taccgtatag ccaagcgtgt ttaaccgctc
     2161 ttgcagccgg tcaccgatca tcaactgtcg ggccaggccc gcaaccgcca tagggtcctg
     2221 ggtcttcgcc ccgcccttgt tcagcagctc gacagcgagg ttcctcaagc gcggttcctc
     2281 ggccaacacc acagcaatgc gcttctggtt gaccatccat atctcgaggg ctgtgacatc
     2341 cgcgtgcagc atggtctgca ggtcgtccga caattcctcc cgcatgacct ggctcagttc
     2401 gctgtcggcc caccaaccca cgcaggcaac caccagcgcc gccgccaacg gcatgatcca
     2461 gttctgcttc gcgcccattg gccaggtcaa tccaggaagg ccgtcaacgg gctggtcggg
     2521 ctcgcggcgt cctgcggcgc ggccagcccg gattcgaacg cggcacgtcc ggcctcgacc
     2581 gccatcttaa aggccgtagc catgcggttt ggatcacttg ccaccgcgat ggcggtgttg
     2641 atcagcacgg catccgcgcc catttccatc gcctcgctcg cgtgactggg tgcgccgagg
     2701 ccggcatcca ccaccaccgg caccacggcc tgctcaatga taatgcgaat caggtctcgt
     2761 gtctgaatgc cgcggtttga gccaatcggc gagccgagcg gcatgacggt ggcagtgccg
     2821 acgtcctgca gccgccgggc caggaccgga tcggcgttga tgtaaggcag cacggtgaac
     2881 ccttccttta ccagagtctc cgccgctttc agcgtctcga tgggatcagg cagcaaatag
     2941 cgcgggtcgg gatggatctc caacttcacc cacttgggca ggccggcggc gaccgccagg
     3001 cgcgccaggc gaacggcctc ctcggcgttc atcgctccgc tggtgtttgg cagcaatagg
     3061 aaccgcttcg gatcaatgaa ctccaggatg ttggcgaagg ggtccttctt gccgctcaaa
     3121 tcagcgcgcc gcaacgcgac cgtgaccatc tcggcgccgc tggcctccaa cgcatcgcgc
     3181 aaggcctcag gcgccgagaa cttgcccgtg ccgaggataa ggcgcgaccg gaacgttcgc
     3241 ccggcgatca ccaatggctg atttgatagg gtcatttggc tcaatgtagt cgcagcggat
     3301 aggcgttgtc gaggcgagcg agcgggcact cgggctggct tgctgtcccg gcgttgccag
     3361 cgtgatgctg gcctgttgcc cccgttatgc ttcgaaggag cttcgaaggg gttacgatgg
     3421 ggcttcgaag gagttacgaa gggattcctt ggaaatcagg cgaccgttaa cgagatgctc
     3481 ctacatgagg tgggatgcgt gagaggcgcg ggatgccgcc gtcctttagc ccccggcgac
     3541 aatcttcaca atctccagcc ggtcgccggc atggagctgg agatgggaga attcagaagg
     3601 cgcgacggcc tcgccgttgt gctcgaccac cacgctgcgc ggcaggagat tctgcgcgac
     3661 gaggaatgcc tcgatcgaac acgggagact ggcgggcact tcctttgcgt tggcgatgat
     3721 tatttggttc tgcattctaa cttgcggctg ggccggttag ctggtgagcg ccatatccca
     3781 atccttccac accggctcat agccgagctg gcgaatcaag tccgccacct ctcgcgggga
     3841 gcggtcatcg gcaatttgga actggctggt ggcgttggcg ggg
//
LOCUS       DATCGS010000182         5765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_66422, whole genome shotgun sequence.
ACCESSION   DATCGS010000182 DATCGS010000000
VERSION     DATCGS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66422"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VIL39_03660"
     CDS             complement(<1..1366)
                     /locus_tag="VIL39_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480551.1"
                     /translation="MVAGGRTVMVSLGLSVQQDAAGVLRGSGVTNIQVGNELVAASYS
                     ISGRVTGGGGNATRATLSVRWLSSGVAVGTNGSFTISVQYNLGVSPGGLSGTATGQAR
                     FAKLGNGTIKSTLSAVPLPAGVNGRWSVNMNLQPPGGTGSVILPNGRSLPASLAGSFS
                     ARSGLELIKLSGVGSDPGSALTINFFPATGAVDSLSGKMLGQSVTIKSLSAKIPSPTV
                     SPASPSPAPSAYAVAQICQECHTPIQQTLNNTRHPQVGVTCQNCHGSLANHAANEYDP
                     ASRPVVDDNVLSTACGSCHSGSQHPIYSEWKASRHASVSVSCPICHEPHQPTSFPDQL
                     RGSLFSTNDSSSVNLCAQCHNDRGASWTTSSAPPRPTPQYNMMLGTVGELESGLPHYD
                     PSYHALFITNQCVGCHMQTAPYRSPTQPAVTGHKFTVDSYGICAGCHGSVVNASNFVV
                     FVTSV"
     gene            1914..2849
                     /locus_tag="VIL39_03665"
     CDS             1914..2849
                     /locus_tag="VIL39_03665"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY5480552.1"
                     /translation="MEHLLANNAAWTKPRRTGEEARVLGITLVLVFLLLGIAVSAMWL
                     YRAAHSGPANVSGEPTGSQPLALSESTKAVLARLDSPLEIRFYALLDPATVPDSLTAF
                     AGRVGQLLSAYQQEAGGKIKVTSFTAQSNPDANAAVADGITVFNLDKGEACYLGVALV
                     LNGRKETLSHLLPEWEKALEPDLTRAIIRLMDAARPVTIPTTVSQINTAAIQEVRALI
                     PDLAAVSVESGKQILRDAALKDFTAAAKEMQAQVKEAEQRLTQAQNGGTDVEQQAAMK
                     HLQQVQTEQTDKLKQIAARSQAQIDALQQIKTVHP"
     gene            complement(3020..3490)
                     /locus_tag="VIL39_03670"
     CDS             complement(3020..3490)
                     /locus_tag="VIL39_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480553.1"
                     /translation="MNRDCCMAAPPSAHDARQSLNASAAAIGAEIYEKYGPRIGWTQL
                     RQILEDRSCVRYPCEIVFDAAPLQAGEFAHPVAKSDHPRDGFVIYVHPLFMMQLEQVP
                     LLVLYQLVLVNYGAFASPDDAETFGAAALGLSKDEYYESLCRLADELQLNTVPD"
     gene            complement(3493..5232)
                     /gene="nrfD"
                     /locus_tag="VIL39_03675"
     CDS             complement(3493..5232)
                     /gene="nrfD"
                     /locus_tag="VIL39_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5480554.1"
                     /translation="MRQAVRNYLVFLWRCGRLAFSGDWRYYTWMGALSVFCLLGLNAY
                     AKQFASGLIVTGMSDEVSWGVYIANFTFLVGVAAAAVMMVIPVYIYNNEELHDLVIFG
                     ELLAVAAILMCLAFVTVDLGRPDRFWHLIPGIGQINFPASMLSWDVLVLNGYLLLNVH
                     ICGYLLYCRYRGRVPAKWFYIPFVFIAIVWAVSIHTVTAFLYVGLGGRPFWNSAIVGP
                     RFLASAFTAGPALIILAMQVVRRVTSFAPVGDASRLRGEMGAGPRPKARAAVLDDLDL
                     LARHLAEMASVPKEVRQSILAGATVLETKTGDVLLRQGETENETFFVLKGRVVVRREE
                     GGRFRITRSLGPGEQFGEISALAGTPRTGTAIAEEPALILSLPVSSLRKLMTTPKMNK
                     IIRSRMSERLLITDRALMTLRSIVQVSMLINVFLLVNEVFKEFYSGNLHVASAKYLFV
                     GLHGHHALVPWIWTAIAFNLTAMVLLVLPVSRSLKWLNLTCVLCIVGIWIEKGMGLVI
                     PGFIPTPLGEMVEYSPSLNETLVCFGIWAFGLLCYTVFLRMSVPILQGRLSKANEGLP
                     GFGVRGAGERSAV"
     gene            complement(5229..>5765)
                     /locus_tag="VIL39_03680"
     CDS             complement(5229..>5765)
                     /locus_tag="VIL39_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5480555.1"
                     /translation="LPHGSLDLEKAEHNYNPELVPEKGYFYMPVQCQQCKNPPCVKVC
                     PVRATWQEADGITVIDYNWCIGCRYCEAACPYWARHFNFTKPSIPKERLNLDMAYLGN
                     RPRRQGVMEKCHFCVQRTRVGRYPACVEVCPVGARKFGNILDPESEVSYILRTKRVFV
                     QLKEELGTSPRFFYYFDV"
BASE COUNT         1293 a         1769 c         1659 g         1044 t
ORIGIN      
        1 tcacactagt gacaaaaacg acaaaattgc tggcatttac gacagacccg tggcaaccag
       61 cacagatgcc ataactgtcc actgtaaatt tgtgacccgt aaccgccggc tgcgtcgggc
      121 tgcggtaggg ggccgtttgc atatggcagc cgacgcactg gttagtaatg aagagcgcgt
      181 ggtaagacgg gtcgtaatgg ggaagccccg attccagctc gccgacagtc cccagcatca
      241 tgttgtattg cggcgtagga cgcggcggcg cgcttgaagt ggtccacgag gcgccgcgat
      301 cgttgtggca ttgggcgcaa agattaacgc tggaagaatc gttggtggag aataaggagc
      361 cgcgcagttg gtcggggaag ctggtgggct ggtgcggctc gtggcaaata ggacaactaa
      421 ctgagacact cgcatgccgg cttgccttcc attcgctgta aattggatgc tgcgaacccg
      481 agtggcatga gccgcacgcc gtgctgagga cattgtcatc aacgaccggt cgggatgctg
      541 gatcgtactc attcgccgcg tggttggcca acgagccgtg gcaattctgg caggtcaccc
      601 caacctgcgg gtgccgagtg ttattcagtg tctgctgaat cggggtgtgg cactcctggc
      661 agatttgagc aacggcgtag gctgagggcg caggtgaagg tgatgccggg gaaacggtcg
      721 ggctggggat ctttgcgctc aggctcttga tcgtgacgga ttggccgagc attttgccgc
      781 tcaggctatc gacagcaccg gtggccggga agaagttgat agtcagcgcg ctgcccgggt
      841 cgctgccaac gcccgagagt tttatgagtt cgaggcccga gcgcgccgaa aagctcccag
      901 ccagacttgc tggcaggctg cggccgttgg gcaggatgac agaaccggta ccgccgggcg
      961 gctgcagatt catgttcaca ctccaacgcc cattcacgcc cgctggcaat ggcacggcag
     1021 aaagggtcga tttgatggtg ccgttgccga gcttcgcaaa tctagcctgg ccagtggccg
     1081 ttccactcag ccccccggga ctgaccccca gattgtattg gactgaaatc gtaaaggagc
     1141 cattagtgcc gacggcgacg cccgatgaca gccagcggac cgataacgta gcacgcgtcg
     1201 cgttgccacc cccacccgtt acccttccgc taatgctgta tgacgcggca acaagctcgt
     1261 tgccaacctg gatgttggtc accccagagc ctcgtaacac tcccgcagca tcctgttgaa
     1321 cagagagccc cagggaaacc atcaccgttc tgccgcctgc caccacgact tcgtggccaa
     1381 attgatacct gcccgtgagg tcataaacga gcgcggtcga gttggtgaac aagaagctgg
     1441 ccggaccaga tggcgccgca ggggcgccga gcgtgttctg cgtgctagcg cacaacacgg
     1501 tggcgaacga aaatgccgac gccaaacaca aaaccgacac ttgcttaccc aacggagtgt
     1561 tcatgccatt cataagccac aagtttcgcc gccgaaagca actattgcac agggcaacaa
     1621 gacacccatt atcagatggt taacagagtt tataccgtcg cactttaaca attcaacgga
     1681 ctcgcacaac accaaactag gtccgggggc caacattctg gcgcggagac taggccggga
     1741 ggcacaaccg ggctatccca atattgtctg atgctcacca ttatttgtgc tggggaacgc
     1801 cgggcgcccc gggcctcccc ttcctgggac caaatttccc tcgcggccgt cgtccgattc
     1861 tgctagaata aaatcagacg gcagcaccag caccaaagca cgaaagagac accatggaac
     1921 atttactggc gaacaatgcg gcttggacca agccgcggcg aacaggggag gaagcccgtg
     1981 ttttgggcat cactctggtc ctggttttct tactgctggg catcgctgtc agcgctatgt
     2041 ggctttaccg cgccgcacac agcggccccg cgaacgtcag tggcgagccg accggctcgc
     2101 agccgttggc attatctgag agcacaaagg ccgtcctggc tcgcctggat tcgccgcttg
     2161 aaatccggtt ctatgctctg ctcgatcccg cgaccgtgcc ggactccttg actgcgttcg
     2221 ccggccgggt gggccaactg ctgtccgcct accagcaaga agccggcgga aagatcaagg
     2281 tcaccagctt caccgcgcag tcaaatcccg acgcgaacgc cgccgtggct gatggcatta
     2341 ccgtcttcaa cctcgataaa ggcgaagcct gttacctggg cgttgcgctg gtccttaacg
     2401 ggcggaagga gacgctgtcc cacctcttgc cggagtggga aaaagccttg gaacccgatc
     2461 tgacccgagc catcatccgg ctgatggatg cagcccgccc cgtcacgatc ccaaccaccg
     2521 tttcccaaat caatactgct gccatccagg aagtgagagc cctgattccc gacttggcgg
     2581 cagtttcagt ggagtcaggg aaacagatcc tccgagacgc ggctctcaaa gacttcaccg
     2641 cggcggcgaa agaaatgcag gcgcaagtca aggaagcgga gcagcgtctg acgcaggctc
     2701 agaacggcgg cacagatgtc gagcaacagg ccgccatgaa gcatcttcag caagtccaga
     2761 ccgagcagac cgacaaactc aagcagatcg ccgcccgctc ccaggcccaa atagacgccc
     2821 tccaacagat caaaaccgtc catccctgac cccgccccct gcgcgccctc agattcgaca
     2881 cgctaccgga attctgaact ggaccgcttc gcccccgact tcattaggta aagctaccgc
     2941 tcatgtagtc tatcccgcag tcttggcagt aacgctacaa aagcctttcc attcttgtca
     3001 gcggccaact attctttgtt cagtcgggga cggtattcaa ctgcaactcg tccgccaacc
     3061 tgcacaggct ttcgtaatac tcgtccttgc taagccccag ggccgccgcg ccgaaggtct
     3121 ccgcgtcgtc gggcgaagcg aatgcgccat aattgaccag gaccagttga tagagcacta
     3181 gtagcggcac ctgttcgagc tgcatcatga acagagggtg cacatatata acgaatccat
     3241 ctcgcgggtg atcgctcttg gcaaccggat gcgcaaactc cccggcctgc aatggcgcgg
     3301 cgtcaaacac gatctcgcac gggtaacgca cgcagcttcg atcttccaga atctgccgga
     3361 gctgggtcca ccctatccga gggccgtatt tctcgtaaat ctccgccccg attgctgcgg
     3421 cagacgcgtt gagtgactgg cgggcgtcgt gcgcgctggg cggggcagcc atgcagcaat
     3481 cgcgattcat gattacacgg ctgaccgctc gccagctccc ctgacgccaa acccaggtag
     3541 tccttcgttg gccttcgaga gacggccctg gaggatgggg accgacatgc gcaggaagac
     3601 cgtgtaacag agcaggccaa aagcccaaat accgaagcaa accagcgtct cgttgagcga
     3661 agggctgtat tcgaccatct cacccagggg tgtggggatg aatccgggga taaccagtcc
     3721 catccccttc tcaatccaga tgccgacaat gcaaagtacg caggttaggt taagccattt
     3781 gaggctgcgg ctgacaggca gcactagcaa caccatggcg gtcaggttga aagcaatggc
     3841 cgtccagatc cacggcacca acgcatgatg gccgtgcagg ccgacaaaca ggtacttggc
     3901 ggaagcgacg tgcaggttgc cggaatagaa ttctttgaac acttcattga ccaggaggaa
     3961 gacgttaatc agcatggaaa cctgcacgat gctgcgaagg gtcatcaggg cgcggtccgt
     4021 gatcaggagc cgctctgaca tccgcgaccg gatgatttta ttcatcttgg gggtggtcat
     4081 aagcttgcgc agggagctga ccggcaggct caggatcagg gcgggctctt cggcgatggc
     4141 cgtgcccgtg cgcggagttc cagcaagggc agagatctcg ccgaattgct ctcccggccc
     4201 aagactgcgc gtaatccgga accgtcctcc ttcctcccgc ctgacaacca cccgcccttt
     4261 caggacaaag aaggtttcat tctcagtctc cccctgacgc agcaacacat cgcccgtctt
     4321 cgtttccagc accgtcgcgc cggccagaat gctctggcga acctccttgg gaaccgatgc
     4381 catttccgcc aggtgccggg caagcagatc caggtcgtcg aggacggcgg cgcgagcctt
     4441 cggtcgtggc cccgctccca tctccccgcg caaccgcgag gcgtcgccca ccggggcgaa
     4501 ggacgttacg cggcggacca cctgcatcgc caggatgatc aacgccggcc cggcagtgaa
     4561 cgccgacgcg aggaaccgcg gcccaacgat ggccgaattc cagaagggtc gccccccgag
     4621 gccgacataa aggaaggcgg tgacggtgtg gatcgaaacg gcccagacga tggcgatgaa
     4681 gacaaagggg atgtaaaacc acttggccgg cacccggcca cgatagcgac aatagagcag
     4741 gtaaccgcaa atatggacgt tcagcagcag atacccgttc agaacgagca catcccagct
     4801 cagcatcgac gccggaaagt tgatttggcc gatgccggga atcaggtgcc agaaccggtc
     4861 tggccgcccg aggtccaccg tgacgaacgc caggcacatg aggatggccg ccacggcgag
     4921 caattcgcca aagatcacca gatcatgcaa ctcctcgttg ttgtagatat agaccgggat
     4981 caccatcatc accgccgccg ctgcgacgcc gaccaggaag gtgaagttgg cgatatagac
     5041 gccccatgac acctcatcgc tcattccggt gacgatcaac ccactggcga actgcttggc
     5101 gtaggcgttg aggcccagca ggcagaaaac agagagcgct cccatccagg tgtaatagcg
     5161 ccagtccccc gaaaacgcca gacggccgca gcgccacaag aacaccagat aattccgcac
     5221 ggcttgcctc atacgtcaaa gtagtaaaag aaccggggcg aggttcccaa ctcctctttc
     5281 aattgcacga acacgcgctt ggtgcggagg atgtaggaga cttcgctctc cgggtccaga
     5341 atgttgccga atttccgtgc gcccaccgga cacacttcga cgcaagcggg ataccggccc
     5401 acccgagtgc gctggacgca gaaatgacac ttctccatca ccccctggcg tcgcgggcgg
     5461 ttgcccaggt acgccatgtc caggttcagc cgctctttcg gaattgaagg cttggtgaaa
     5521 ttgaaatgcc gggcccaata gggacacgca gcttcacaat accgacagcc gatgcaccag
     5581 ttgtagtcaa tcaccgtgat accgtccgcc tcctgccagg tcgcacgcac ggggcaaacc
     5641 ttcacgcacg gcgggttctt gcactgctgg cattgcaccg gcatgtaaaa atatcccttc
     5701 tctggaacca gctccgggtt gtaattatgt tccgcctttt ccaagtccag cgacccgtgc
     5761 ggcag
//
LOCUS       DATCGS010000183         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_116196, whole genome shotgun sequence.
ACCESSION   DATCGS010000183 DATCGS010000000
VERSION     DATCGS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116196"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VIL39_03685"
     CDS             complement(<1..626)
                     /locus_tag="VIL39_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HEY5480556.1"
                     /translation="MMQTTTVPKPSPNEILKAGSPTLSGTIAQTLADPQADHFVEDDA
                     QFLKFHGVYPQDDRDLRKTGKKYIFMVRCRIPGGLLTPAQYLACDDLSARYANNTLRV
                     TSRQGFQFHGVVKGGLRALVKEINDALLGTLAACGDVSRNVMAPPTPAMNNLGAQVQE
                     HARQAAAAFLPRTRAYHSIWIEGVPLDLEDPANKDFVDPLYGKTYLPR"
     gene            complement(643..897)
                     /locus_tag="VIL39_03690"
     CDS             complement(643..897)
                     /locus_tag="VIL39_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEY5480557.1"
                     /translation="MDPNPPKPTVGDNHFRSLAKAISWRATGTLDTIAVSFIITGRIK
                     LALSIGCVELFTKICLYYGHERVWNRIPLGKVKAREDYEI"
     gene            complement(926..1888)
                     /gene="cysK"
                     /locus_tag="VIL39_03695"
     CDS             complement(926..1888)
                     /gene="cysK"
                     /locus_tag="VIL39_03695"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY5480558.1"
                     /translation="MPVYPDVISTVGRTPLIKLNRIANGAVATIALKAEFFNPLGSVK
                     DRIGYAMIAAAERDGILTPQTTIIEPTSGNTGIALAFVAAAKGYKLILTMPESMSIER
                     RTLLALLGARVVLTPATEGMKGAIKRAEDLHKEVADSWIPQQFDNPANPEIHRRTTAE
                     EIWKDTNGKADILVSAVGTGGTLTGISEVLKSRKKGFRSIAVEPKDSPVITQTRNGEP
                     LKPGPHKIQGAGAGFIPKNLNLQIVDDVITVSNEDAFAVARQLALEEGILAGISTGAN
                     VWAALQAAKRPENKGKLIVTIGCDTGERYLSTALADEARKRVQV"
     gene            complement(1955..2779)
                     /locus_tag="VIL39_03700"
     CDS             complement(1955..2779)
                     /locus_tag="VIL39_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY5480559.1"
                     /translation="MPLPAIDSLPSKPALPARRSIAELLAPLDRIAAESPNLVANHGA
                     RFESDGQTCELPRYLFIGPKGGDDMIRVGIFAGLHGDEPEGVHALIQFLSLLEDKPEL
                     ATGYCLFLYPVCNPTGFEDRTRHARTGKDLNREFWNGSVEPEVRLLQSELVAHSFHGI
                     ISLHTDDSSQGFYGFAHGATLTKNLVEPALQAAEQFLPRNGNEKIDGFRARKGIIRDT
                     YPGVLSAPPKVRPRPFEIILETPQTPPNYLKEAAFVAALRMILTRYREFIAYAPNL"
BASE COUNT          571 a          966 c          966 g          733 t
ORIGIN      
        1 ttgcgcggca ggtaggtctt gccgtagagc ggatcaacaa agtccttatt cgccgggtct
       61 tcgagatcga gcggcacccc ctcgatccag atcgaatgat aggcccgcgt gcgcggcaag
      121 aacgccgccg ccgcctggcg cgcgtgctcc tgcacctgcg cgcccagatt attcatggct
      181 ggcgtgggcg gggccatcac attgcggctg acgtcgccgc aggccgcgag cgtgcccagc
      241 agcgcgtcat tgatctcctt caccagcgcg cgcaagccgc ccttgaccac cccgtgaaac
      301 tggaatccct gccgggaggt cacgcgcagc gtgttgttgg catagcgcgc ggacaggtcg
      361 tcgcaagcca ggtattgggc cggggtaagc aggccgccgg gaatgcggca gcgcaccatg
      421 aagatgtact tcttgcccgt cttgcgcagg tcacgatcgt cttgcggata gacgccgtgg
      481 aacttcagaa actgcgcgtc atcctcgacg aagtggtcgg cttgcgggtc ggccagcgtc
      541 tgggcgatcg tacccgagag cgtcgggctg ccggctttca ggatctcatt cggcgatggt
      601 ttcgggacgg ttgtagtctg catcataatc actcctctgt cgttaaatct cgtagtcttc
      661 cctcgccttc acttttccca aaggtatccg gttccatacc cgctcgtggc cgtagtagag
      721 gcagattttc gtgaatagtt cgacgcaacc gatagagagc gccagtttga tccgccccgt
      781 gatgatgaac gaaacggcga tggtatccag cgtgccagta gcccgccacg aaatggcctt
      841 cgccagactg cggaagtggt tgtcccccac ggtcggcttg ggaggattgg gatccatgcg
      901 gttccattgg gaacgcggct gcgtttcaca cttgcactcg ctttcgtgct tcgtccgcca
      961 acgctgtgct cagataacgc tcacccgtgt cacatccgat cgtcacaatc aatttgccct
     1021 tgttttccgg acgcttggcc gcttgcagcg ccgcccatac gttggccccg gtcgaaatgc
     1081 cggccaaaat accctcttcc agcgccaact gtcgcgccac cgcaaatgcg tcctcgttac
     1141 tcactgtaat gacatcgtcc acaatttgca ggttgaggtt cttggggatg aaccctgccc
     1201 ccgcgccctg aatcttgtgc ggccccggct tgagtggttc gccattgcgc gtctgcgtga
     1261 tgaccggcga atcttttggc tcgacagcga tggaccggaa gcctttcttc cgcgacttga
     1321 gcacttcgct gatgcccgtg agcgtgccgc cagtgccgac cgccgagacc aggatgtcgg
     1381 ccttgccgtt ggtgtccttc caaatctcct cggccgtggt ccgccgatga atttccgggt
     1441 tggccgggtt gtcgaactgc tgcggaatcc atgagtcggc gacttccttg tgcaagtcct
     1501 ccgccctttt gatggctcct ttcatgccct cggtggccgg ggtgaggacc accctggccc
     1561 cgagcagcgc cagcaatgtg cgccgctcaa tgctcatgct ttcgggcatt gtcaggatca
     1621 gtttgtaacc cttggccgcc gccacgaaag cgagcgcaat cccggtgttg cccgaggtcg
     1681 gctcgatgat cgtggtctgg ggcgtgagga tgccgtcgcg ctcagcggcc gcaatcatgg
     1741 cgtagccgat ccggtccttc acgctgccga gcgggttgaa aaactcggcc ttcaacgcaa
     1801 tggtcgcgac cgcgccattg gcgatccggt tgagtttgat cagcggggtc cgccccactg
     1861 tgctgataac gtctggatat actggcatag ttactttcct ttctctgttg ttgacttgtt
     1921 taacttattg aactctttta aatcattgcg cgagttataa attcggcgcg taggcgatga
     1981 actcgcggta ccgcgtcagg atcatccgca gggccgccac aaaagccgct tccttgaggt
     2041 aattcggcgg cgtctgcggt gtttccagga tgatctcgaa gggccgcggc cgcactttgg
     2101 gcggcgcgct cagcactccg gggtaagtgt cgcggataat gcccttgcgg gcgcgaaaac
     2161 cgtcaatctt ctcattcccg ttgcgaggca ggaactgctc cgcggcttgc agcgcgggct
     2221 cgactagatt cttcgtcaac gtcgccccgt gggcaaagcc atagaagccc tggctgctgt
     2281 cgtcggtatg caacgagatg atcccgtgga acgagtgcgc caccagttcc gactggagca
     2341 gcctcacctc cggctccacc gatccgttcc agaactcacg gttcaagtcc ttcccggtgc
     2401 gcgcatggcg tgtccggtct tcaaacccgg tcgggttgca gaccggatat aggaacaggc
     2461 aatagccggt ggccagctcc ggcttgtcct ccagcaggct taggaactgg atgagggcat
     2521 gcaccccctc cggttcgtca ccgtgaaggc cggcgaatat cccgacgcgg atcatgtcgt
     2581 cgccgccctt cgggccgatg aacaagtacc gcggcagttc gcaagtctgt ccgtcactct
     2641 cgaaccgtgc gccgtggttg gcaacgaggt tgggcgattc cgccgcgatt cggtcgagcg
     2701 gcgcgagcaa ctcggcaatc gaacgtctgg cggggagggc cggtttggag ggcagggagt
     2761 caattgcggg taaaggcata ttattgatca agttgactgg tttagtcacg gaacgaacgg
     2821 tcgtcagacc ggctggtgcc gagccaatcg gtcgaatctg gcctaagtgc cactgatgat
     2881 ttcataatca agatgcgcta aagatgagtg cagtataggc ataagcatgt gtatgtcaat
     2941 taagtaactc cagtttgcat ctgtgctgat tatcattggg gtgttatgtc acttccgctt
     3001 atctccactt gttgtctctt tgccgggcgc atcccgatac caccaccaag gcgggcggct
     3061 ttgccaatcg gaacgccgga tttattcggc agccccagtg cggctcgccg gcctgccgga
     3121 tgaatccggc gttccggtgg tggggtcagg atgcgcccct ctttgtctgt gggtttgaca
     3181 ggtaggtttc cagtctgcta ttctcggcag cgttgacaca atgaaacatc cttctt
//
LOCUS       DATCGS010000184         5749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_15440, whole genome shotgun sequence.
ACCESSION   DATCGS010000184 DATCGS010000000
VERSION     DATCGS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15440"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..850
                     /locus_tag="VIL39_03705"
     CDS             161..850
                     /locus_tag="VIL39_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5480560.1"
                     /translation="MSEILLSATGIHKTYTLGRRSLDVLRGVDVELRRGEFLALRGAS
                     GAGKSTLLHLLGGLDTPDQGEIRLAGRNLATLGRRDLARLRNEEVGFIFQSYYLLPEL
                     DALENVCLPARMARTPAAKAEARGRELLARVGLKDRLEHKPYELSGGEQQRVAIARAL
                     INEPDLILADEPTGNLDSHTGEEIIELLCALRDERQTTLVMATHDARLAARAPKVVEL
                     VDGVIADGKTE"
     gene            complement(942..2915)
                     /locus_tag="VIL39_03710"
     CDS             complement(942..2915)
                     /locus_tag="VIL39_03710"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY5480561.1"
                     /translation="MQDERKTPDDRNKMTDLERLRHSCAHVMATAILRLWPDAQFAAG
                     PAVENGFYYDVELQHRITPEDFPAIEAEMKKEIKANRTFEKVVVTREQAIKDSESGRL
                     GGLSERPGNPSKFKLGNLEAIPDGEAITYFKSGDFIDLCAGPHVMRTGNIGAYKLTSV
                     ASAYYKGDEKNPQLQRIYGTAFKNKTELEAYFTMLEEAKRRDHRKIGQEMGLFTIDAE
                     FTGPGLPLWLPKGGAILEELEKLAKETEFAAGYVRVKTPHIAKEKMYKTSGHLPYYAE
                     SMFPPMELVEEEDRPDKRFLEARVQFAIQQKQELAGTGDVEKIAHWDKQQAEAAEAID
                     KLTTRYYLKAMNCPHHHRIFAAEPRSYRDLPLRLAEYGTCYRYEQSGELFGLMRVRSL
                     NMNDSHIYCTAEQFAQEFNAVNEMYLKYFKIFGIEKYVMRFSTHGKEGLGKKYVNEPE
                     LWIQTENMVRDVLKTSGINFVEVANEAAFYGPKIDVQVWSVIGREFTLATNQVDFAVP
                     RKFGLVYRDKDNTEKTPLCIHRAPLGTHERFIGFLIEHYAGNFPLWIAPEQVRVLTIG
                     EEEPLVSYAKAIVQELRANMVRAEGDYSNDKINGKIQQAELAKVHTMLVVGPRDMAAN
                     AVSVRVHGKGSLGAKPKAEVVADILAAVRERRA"
     gene            complement(3034..4593)
                     /locus_tag="VIL39_03715"
     CDS             complement(3034..4593)
                     /locus_tag="VIL39_03715"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY5480562.1"
                     /translation="MKKPLAPAVEPALSRGLRRYLYFTAATTGAAIMIVEILGAKMLS
                     PYIGLSHFVWTAQIAVTLVALACGYYVGGRMADRSQRLAGLYWAILSAALYLALTVWI
                     CEPVAYWCLDFNLAVGSLLASAILFFVPLALLAMTGPFLVRVITSSVAGVGGNVGRLT
                     SVGTLGSLAGTLLIGYLLIPLLPNSLTMCFTALALMLVCAGYFLLCRRKGPAVLVLLL
                     ALSPALGGKSYLGLTHNFAYVTELFRGNSHFGLLQVVDRRDGGCRFYLNDGLTQNTYD
                     PVKKLSVSHFTYALAGLARAYTTNINDVLCIGLGVGIVPMEFARQGARVDVVEINPAV
                     VPVAVRFFDLETNKLNLTLDDGRHFLNRCRKQYDVVVLDAFLGDSSPSHLMTREAFAS
                     IRRVLRPGGTLVINSFGNLEDGKDFFAASLNLTLKAVFPGVRIHTSGDGGIFFAASDR
                     PALEFVHPPNLENIHPDVLRDTKATYAGIVDMIPGHGRVLTDDYNPAEFYDARNREDL
                     RRRLAMAAREM"
     regulatory      4704..4787
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            4813..5208
                     /gene="crcB"
                     /locus_tag="VIL39_03720"
     CDS             4813..5208
                     /gene="crcB"
                     /locus_tag="VIL39_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY5480563.1"
                     /translation="MWSALWIFVGGGLGSLARWGASGLIASKFGETFPWGTLCVNVTG
                     SFIIGLFATATGPEGRWMAPASFRQFFMLGICGGYTTFSSFSLQTLNLAEDGQWFKAG
                     ANSVLSLVLCLVGVWLGSLLASVINSSSR"
     gene            5289..5645
                     /locus_tag="VIL39_03725"
     CDS             5289..5645
                     /locus_tag="VIL39_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY5480564.1"
                     /translation="MHLPQEAMLLRIFIGESDRWHHQPLYEAIVLKARELHLAGATVL
                     RGPMGYGKSSRLHTAKILQLSMDLPLVIEIVDAEEKIQAFLPVLDEMMKGGLVTLEKV
                     RVIDYRSDPDLEEPKI"
BASE COUNT         1056 a         1705 c         1821 g         1167 t
ORIGIN      
        1 ctttttccgg cttccattta tcagtttacc gaattaccgg cgctgattgt ccggcaggac
       61 atcctgctga tctgcggcag cgcctgggtg atctgcaccc tgggcggatt gattccagca
      121 tggcgggcgg gccgcctcaa acccgtggag gcgctgcgct atgagtgaga ttctcctctc
      181 agccactggc atccacaaga cctacaccct cggacgacgc tcgctggacg tgttgcgcgg
      241 cgtggacgtg gaactgcggc gtggggagtt tctcgccttg cgaggcgctt ctggcgcggg
      301 caagagtacg ctcctccatc tgttgggcgg actggatacg cccgaccagg gtgaaatccg
      361 gctggccggc aggaacctgg ccaccctcgg gcgccgggac ctggcgcggt tgcgcaatga
      421 ggaagtgggc ttcattttcc agtcctacta cctgttgccg gaactggacg cactggaaaa
      481 cgtctgtctg cccgcgcgta tggcgcggac gccggcggcc aaagccgagg cgcgcggacg
      541 cgaattgctg gcgcgcgtgg gcctcaaaga ccgcctggag cacaagccct acgagctttc
      601 cgggggcgag cagcagcgcg tcgccattgc gcgggcgctg atcaacgagc cggacctgat
      661 tctggcggat gaacccaccg gcaacctcga ttcgcatacc ggcgaggaga tcatcgaact
      721 gctttgcgcc ctacgcgacg agaggcaaac caccttggtc atggctacgc acgatgcgcg
      781 cctggccgcg cgcgccccca aggtcgtgga gctggtggat ggggtaatcg cggatgggaa
      841 aacggagtag gctccggctt gccggacctg atgcccaagc tcgaggccat tctcggccag
      901 gttccgtagt ctgaaactgc ctggtcagaa gggcatcggt cctacgctcg ccgctcgcgg
      961 actgcggcca ggatatccgc cacgacttcg gccttcggtt tggccccgag gctgccttta
     1021 ccgtggacgc ggacgctgac agcgttagcg gccatgtcgc gcgggcccac caccagcatg
     1081 gtgtggacct tggccaactc ggcttgctgg atcttgccgt tgatcttgtc gttgctgtaa
     1141 tcgccctcgg cgcggaccat gttcgcccgc agttcctgca cgatggcttt ggcgtagctc
     1201 accagcggct cttcctcgcc gatcgtcagc acgcgcacct gttcgggcgc gatccaaagc
     1261 gggaagttgc cggcataatg ctcgatgagg aagccgatga accgctcatg cgtgccgagc
     1321 ggggcgcggt ggatgcacag cggggtcttc tcggtgttgt ccttgtctcg atacaccaaa
     1381 ccgaacttgc ggggcacggc gaaatccacc tggttggtgg ccagcgtgaa ctcgcggcca
     1441 atcacactcc acacctgcac gtcaatcttc ggcccgtaga acgccgcttc gttggccacc
     1501 tcgacgaagt tgatgcccga ggttttgagc acgtcgcgca ccatgttctc ggtctgaatc
     1561 caaagctcgg gttcgttgac gtatttcttg cccaaccctt ccttgccgtg ggtgctaaac
     1621 cgcatcacgt atttctcgat gccgaagatc ttgaagtact tgagatacat ctcgttgacg
     1681 gcgttgaact cctgcgcgaa ctgctcggcg gtgcagtaga tgtgcgagtc gttcatgttt
     1741 agcgagcgca cgcgcatcag gccgaacagc tcgccggact gctcgtagcg gtagcaggtg
     1801 ccgtattcgg cgaggcgcag gggcaagtcg cgatagctgc gcggctccgc ggcaaagatg
     1861 cggtggtggt gcgggcagtt catggccttg aggtagtagc gcgtcgtgag cttatcaatg
     1921 gcttccgcgg cttcggcctg ctgcttgtcc caatgagcaa tcttctcgac gtcgccagtc
     1981 cccgccagtt cctgcttctg ttggatggcg aactgaacgc gcgcctccag gaaacgcttg
     2041 tccgggcggt cttcctcctc aaccagctcc atcgggggaa acattgactc cgcataatac
     2101 ggcaagtgcc cggaggtctt atacatcttc tccttggcga tatgcggcgt cttcactcgc
     2161 acgtagcccg cggcgaactc cgtctctttg gccagcttct ccagctcttc cagaatagcg
     2221 ccgcccttgg gcagccacag cggcaagccg gggccggtga attcggcgtc aatggtgaac
     2281 agccccatct cctggccgat cttgcgatgg tcgcggcgct tggcctcctc cagcatcgtg
     2341 aagtaagcct ccagctcggt cttgttcttg aacgcggtgc cgtaaatgcg ctggagctgc
     2401 gggttcttct cgtcgccttt gtaataggcg ctggcgacgc tggtgagctt gtaagcgccg
     2461 atgttgccgg tgcgcatcac gtgcggcccg gcgcacagat caatgaagtc gccgctcttg
     2521 aagtaggtga tagcctcgcc gtcggggatg gcctccaggt tgccgagctt gaacttgctc
     2581 gggtttcccg gccgctcgct caagccgccg agccggccgc tctccgagtc cttgatcgcc
     2641 tgctcgcggg tgacgacgac cttctcgaag gtgcggttgg ccttaatctc cttcttcatc
     2701 tcggcctcta tggccgggaa gtcttccggc gtgattcgat gctggagttc cacgtcatag
     2761 tagaacccgt tctcgacggc tggtccggcg gcgaactggg cgtccggcca caggcgcagg
     2821 atggcggtgg ccatcacatg ggcgcaggag tggcgtagcc gctccaagtc ggtcatcttg
     2881 ttgcggtcgt cgggcgtttt cctttcgtct tgcatgaggg gaatttaacc acagatggac
     2941 acagatggac acagataagt tgttacagga cgatgcgttc ccactccagc tttgcgtgct
     3001 tgaagttcag gatcacttcc atctgtggtt acttcacatc tccctcgctg ccatggccag
     3061 gcggcggcgg aggtcttcac ggttccgggc atcgtagaac tcagccgggt tgtagtcatc
     3121 ggttagcaca cgtccatgtc cagggatcat gtccacgatg ccggcgtagg tggctttggt
     3181 gtcgcgcagg acatccggat gaatgttctc caggttcggt gggtgcacaa actccagggc
     3241 cggccgatcc gaggccgcga agaagatgcc tccgtccccg ctggtgtgaa tccgcacgcc
     3301 cgggaagacc gctttcaagg tcaggttcag tgaggcggcg aagaagtcct tgccgtcttc
     3361 caggttgccg aagctattga tgaccagggt cccgccgggg cgcaacactc gccggatgga
     3421 ggcaaacgcc tcccgcgtca tcagatgcga aggcgacgag tcgcccagga aggcgtccag
     3481 cacgaccacg tcgtactgct tgcggcagcg gttcaggaaa tggcgtccgt cgtccagcgt
     3541 caggttgagc ttgttggttt cgaggtcgaa gaagcgaacc gccactggca ccacggcggg
     3601 gttgatttcc accacgtcca cccgcgcgcc ctggcgggcg aattccatcg gcacgatgcc
     3661 aacgcccagg ccgatgcaga gcacgtcgtt gatgttggtc gtgtaggccc gcgccaatcc
     3721 cgcgagggcg taggtgaaat gggagacgct gagcttctta accggatcgt aggtgttctg
     3781 ggtgagccca tcgttcaggt agaaacggca gccgccgtcg cgccgatcaa ccacctggag
     3841 caggccgaaa tgagagttgc cccggaataa ctcggtcacg taagcgaagt tatgggtcag
     3901 acccaggtag ctcttcccgc cgagcgccgg actcagcgcg aggagcagga ccaggaccgc
     3961 cggccccttg cggcggcaga ggagaaaata gccggcgcaa accagcatca gcgccagcgc
     4021 cgtgaaacac atcgtgagcg agttcggcag gaggggaatc aagagatagc cgatgagcag
     4081 cgtgcccgcc aggctgccca atgtgccgac ggaagtgagc cgcccgacgt tgccccccac
     4141 gccagccacc gacgaggtga tcacgcgcac caggaacggc cccgtcattg ccagcaacgc
     4201 gagcggaaca aagaacagga tcgccgaagc cagcagggag ccaacggcga ggttgaagtc
     4261 gaggcaccaa taggcgaccg gttcgcagat ccataccgtc agggcgaggt aaagcgccgc
     4321 gctcaggatc gcccaatata gccccgcgag ccgttgcgac cggtccgcca tccgcccgcc
     4381 cacatagtag ccacaggcca gagctaccag cgtcacagca atctgcgccg tccaaacgaa
     4441 gtgcgacagg ccgatgtaag gggagagcat cttggcgccc aggatctcca cgatcataat
     4501 cgcggcgccc gtggtcgcag cagtgaaata caggtagcga cgcagccccc gcgacagagc
     4561 gggttccacg gcgggtgcga gtggcttctt cacagcgcag gttttacgcg acgcccgcgc
     4621 cgggggcaat gtttttaccg ttttggactg gaacccgggc aagggcagtt tttttattgc
     4681 tggcgggccg ggttggcagt atcgtcggcg gtggcaatgg agtttgccgt ctcgcgcagc
     4741 gagagaactg cctgatcggt ttcagcgctg ataactccta ccagcgccat gtgtcgtggc
     4801 ggggttggat ttatgtggag tgcactatgg atatttgtcg gtggcggctt gggcagcttg
     4861 gcccgatggg gagcttctgg cctcattgcc agcaaatttg gcgagacttt cccgtggggc
     4921 acgctttgcg taaacgtcac aggctcattc atcatcgggc tgtttgccac ggcaaccggg
     4981 cccgaggggc ggtggatggc accggcttct ttccggcagt tcttcatgct cggaatctgt
     5041 ggcggataca caaccttttc ctctttcagt ctgcaaacac tcaacctggc cgaggacggc
     5101 cagtggttca aggcgggcgc caactcagtg ctttcgctgg tgctgtgcct ggtgggcgtt
     5161 tggctgggaa gtctcctggc ctcggtaatt aactccagtt cacgatgaaa ccttgcccgc
     5221 ctgctattgc gtcgctgttc tctccggcac ttcctcactc aatcacgtga tcaacccatt
     5281 cttttgatat gcatctgccc caggaagcaa tgttgctgcg gatcttcatc ggcgagagcg
     5341 accggtggca ccaccaaccg ctttacgaag cgatcgtgct gaaggcgcgg gagctgcacc
     5401 tggcaggggc aaccgtgctg cgtgggccga tgggctatgg gaaatcgagc cggctgcaca
     5461 cggcgaagat tctgcaactg tcaatggatt tgccgctggt aatcgagatt gtggatgccg
     5521 aggagaagat ccaggcgttt ctgccggtgc tggatgagat gatgaaaggg gggctggtga
     5581 cgctggaaaa ggtgcgggtg attgattatc ggtcggaccc ggatctggag gagccgaaga
     5641 tttgaaccgg ccttcacggt gccactgatt tgagggaagc agaaaaagct ttattgcctc
     5701 gatcaaatgc ctgaaaagat agggatgaaa atgattcaaa ccgtccgct
//
LOCUS       DATCGS010000185         4116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_413340, whole genome shotgun sequence.
ACCESSION   DATCGS010000185 DATCGS010000000
VERSION     DATCGS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413340"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIL39_03730"
     CDS             complement(<1..396)
                     /locus_tag="VIL39_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5480565.1"
                     /translation="MNWIAWALLSAFFAGITAILAKIGVAGVDSNLATAVRTTVVLVF
                     TWGIALATSPASSLRQLSNRTLLFLALSGIATGLSWLCYFRALQMGQASRVAPVDKLS
                     VVFAIVLAAVVLGEKLQWQHYVGGALIVTG"
     gene            752..>4116
                     /gene="mfd"
                     /locus_tag="VIL39_03735"
     CDS             752..>4116
                     /gene="mfd"
                     /locus_tag="VIL39_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY5480566.1"
                     /translation="MFASVSETPAVQSLARRLEEGGVLSCAGISAAAQPFLAALLQHL
                     FPHRPIVVVTEGLRTQESTQQDLATWLAMGSEVQSLKSKVQSQAIGDRNQESESSIQN
                     HASRITHHASSTPQPLFYPSWEVLPHEVRLPHSDVISERLETLVALTHHASRLTHQAP
                     LVVTSVVALLQRTFPAGTVRERTRTLALGDRADPLDLVEWLEEQGYEPEAQVTQKGEI
                     ALRGGILDIYPLTSPWPVRMEFFGDELESLRQFDPLTQMSREEITAVTIPPAGELGLL
                     KQMRNAECGVRSENPQPPLGTLLDYLPPETVFLLCEPEILAERADEYAQQVPDGDPFF
                     IPWGTFQEQAGQRGMTRLEVSEAESVLVDMGGDQEAALDSPAALELPGASGISHSALR
                     TPHFESLEAFRPLPERAPEPQVAEAQRREFFAQLHRWLRQGYGVHVFCNNDGERQRFT
                     EIWDEYGLAEEGPKTDVQRPTVPDTAGLWTLDFRLWTHLGALARGFLCDEARLVVVTD
                     AEIFGRYKVQRPRRLKSLHAQAARSALDIDFTELEEGDYVVHLQHGVGRYVGLQVLPL
                     GAGTKPTEGPGANAHAGQECLVIEYASGDPAQPPPKLYVPVTEAHLVSKYVGTGKARP
                     PLNTLGGTRWAKAKAHAERAVRDVAGDLLSIQAARESQPGHPCAPDTPWQREFEGAFI
                     FEETPDQQRAIVETKADMERPKPMDRLICGDVGFGKTEVGIRAAFKAVMDGKQVAVLV
                     PTTVLAQQHYNTFRERMADYPIRVELLSRFRTRRAQDLVVRDLATGSVDIVIGTHRLL
                     QNDIVIKDLGLVVIDEEQRFGVMHKEKFKMLRRLVDVLTLSATPIPRTLYLALTGARD
                     MSTIQTPPHDRLPVETIVTQYDERVIRDAIQRELNRGGQVFFLHNRVLTIQTMAQKLS
                     GLLPNARILVGHGQMHSDELEEVMTKFVNGEADVLLSTTIIESGLDIPNANTIIIDRA
                     DRFGLSDLYQLRGRVGRYKHQAYAYLLLPRHGNLLTDVRKRLSAIKQFSTLGSGFKIA
                     MRDLEIRGAGNLLGAEQSGHITAVGFELYCQLLKQSVASLKGEKVKPRVEVRVNLDFL
                     ALSPGEESPKSKVQSPKSGGGEPESASRITHHAS"
BASE COUNT          841 a         1282 c         1271 g          722 t
ORIGIN      
        1 gccggttacg atgagcgcgc cgcctacata atgctgccac tgaagctttt ctcccagcac
       61 gaccgccgcc agcacaatgg caaacacgac gctcagcttg tcaaccggcg cgacgcggga
      121 tgcctgtccc atttgcaagg cgcggaaata gcacagccat gagaggccag tggcgatgcc
      181 ggaaagggcc aaaaacaaca gcgtccggtt ggacaactgg cggagcgacg acgccgggct
      241 ggtcgccaaa gcgatgcccc aagtaaacac cagaaccacg gtagtccgta ccgccgtggc
      301 caggttggaa tccactccag caacgccgat tttggctaga atggcggtaa tgccagcgaa
      361 gaaggcggac aacaaagccc aagcaatcca gttcatggcg gagagcctgc tcgattcagc
      421 actaccggtc aacccgcctt gacacccgcc ttgacatatg gcggagtgga agtcctggaa
      481 agcaatcaaa gtcgaaacag caaatgctca agccgcagtc agtcaggccc agcgttcggc
      541 acaaccctca accttttgat ttcagctcat tctatgtcat cttttgtcat ccgtccgccg
      601 gccctgcgtt ggccaataca acgccgagag caggcccttt tacccactaa agactcattc
      661 aatccttctt tgccgtcctt gacgcccctg cgcctgcctg cgaggttgtc cttgcttggc
      721 gcaagacttg gcaatcgcgg cgacaagcga attgttcgct agcgtctcgg agactcccgc
      781 ggtgcagtcc ctggcacggc gcctggagga ggggggagtg ttgtcttgcg cgggcatcag
      841 cgccgcagcc cagccttttc tcgctgcgtt gctgcagcat cttttcccac accggcccat
      901 cgtcgtcgta accgaaggac ttaggacgca ggaaagtaca caacaggatt tggcgacgtg
      961 gctggcgatg gggagcgaag tccaaagtct aaagtctaaa gtccaaagtc aggcgatagg
     1021 agacaggaat caggagtcgg aatccagtat ccagaatcac gcatcacgca tcacgcatca
     1081 cgcctcctcg actcctcaac ccctgttcta tccgtcctgg gaagtcctac cccacgaggt
     1141 ccggctgccg cactcggacg tcatcagcga gcgcctggaa acgctcgtcg ccctcacgca
     1201 tcacgcctca cgcctcacgc atcaagcccc tctcgtcgtt accagtgtcg tcgccctgct
     1261 ccagcgcacg ttcccggccg ggacggttcg cgagcgcact cgcacgctcg cgcttggtga
     1321 ccgtgccgat ccgctggatt tggtcgagtg gctcgaagag cagggttatg aaccggaggc
     1381 gcaagtgacc cagaaagggg agattgcttt gcgcggcggc attctcgata tctacccact
     1441 caccagcccc tggccggtgc gcatggagtt ctttggtgat gagctggagt cgctgcggca
     1501 gtttgatccg ctgacccaga tgtcacgaga ggagatcacg gcggtcacga ttccgccggc
     1561 cggggagttg ggactcctca agcaaatgcg gaatgcggaa tgcggagtgc ggagtgaaaa
     1621 ccctcaaccc cctctgggaa cgttgctgga ttatctgccg ccagaaacag tatttctgct
     1681 gtgcgagccg gaaatcttgg ctgagcgggc cgacgagtat gctcagcaag tgcctgacgg
     1741 ggaccctttc ttcattccgt ggggaacgtt tcaagagcag gctgggcagc gcggcatgac
     1801 ccgcctggag gtcagtgaag ccgagtcggt tctggttgat atgggaggcg atcaggaagc
     1861 cgctttagat agtcccgcag cgcttgaact acccggggca agcggcatta gccactccgc
     1921 actccgcact ccgcacttcg agagcttgga ggctttccgt ccgctgccgg agcgggcgcc
     1981 ggagccgcag gtcgctgagg cgcaacggcg agagttcttt gcccaactgc accgctggtt
     2041 gcgccagggt tatggagtgc acgtcttctg caataacgac ggcgaacggc agcgcttcac
     2101 tgagatttgg gacgaatacg gcctggcgga ggagggcccg aagacggatg tccaaaggcc
     2161 aacggtgccc gataccgcag gactttggac tttagacttt agactctgga cccatctagg
     2221 cgcgctggcc cgcggcttcc tttgcgacga ggccaggttg gtagtggtca ccgacgcgga
     2281 gatctttggg cgttacaagg tgcaacggcc acgccgcctc aaatcgcttc acgcacaggc
     2341 cgcgcgctcg gccctggaca ttgatttcac ggagcttgag gaaggcgact atgtcgtcca
     2401 cttgcagcac ggggttgggc ggtatgtcgg gctgcaggtg ttacccctgg gcgcgggcac
     2461 gaagccaacc gaagggccgg gtgcgaacgc ccacgccggc caggaatgcc tggtcatcga
     2521 atacgcgtcg ggcgaccccg cgcagccacc gccgaagctg tatgtgccgg taaccgaagc
     2581 gcacctggtc agcaaatatg tcggcacggg caaagcgcga cctcccctca acacgctggg
     2641 cggcacccgc tgggccaagg ccaaggccca tgccgaacgg gcggtgcgag acgttgccgg
     2701 cgacttgctc tccatccagg ccgcgcgcga atctcaaccg ggccatccct gcgcaccgga
     2761 cacaccctgg cagcgcgagt tcgagggcgc cttcatcttc gaggagacgc ctgaccagca
     2821 acgggccatc gtggagacga aggccgatat ggaacgtccg aagcccatgg accggttgat
     2881 ctgcggcgac gtggggttcg ggaagaccga agtcggcatc cgcgctgcgt tcaaagcggt
     2941 gatggacggc aagcaggtcg ccgtgctggt gccgacgacc gtgctggcgc agcagcacta
     3001 caatactttt cgcgagcgca tggccgatta cccgatccgg gtggagttgc tctcgcggtt
     3061 ccgcacccgc cgggcccagg atctggtcgt ccgcgacctg gcgaccgggt ccgtagatat
     3121 tgtgatcggc acgcaccggc tgttgcagaa cgacatcgtc atcaaagacc tggggctggt
     3181 ggtgattgac gaggagcagc gctttggcgt gatgcacaag gagaagttca agatgctgcg
     3241 ccgcctggtg gacgtgctca cactcagcgc caccccgatc ccccgcacgc tatacctggc
     3301 gctgacgggc gcgcgggaca tgagcaccat ccaaacgccg ccgcacgacc ggttgccggt
     3361 cgagacgatc gtcacccaat acgacgagcg cgtgatccgg gacgccattc agcgcgagtt
     3421 gaaccgcggc gggcaggtct tcttcctgca caaccgcgtg ctgacgatcc agaccatggc
     3481 ccaaaagctg agcgggctcc tgccgaacgc ccgcatcctc gtcggccacg gccagatgca
     3541 cagcgacgag ctggaagagg tgatgaccaa gttcgtcaac ggcgaggccg acgtgctgct
     3601 gtccaccacg atcatcgaga gtggcctcga tattccgaat gccaacacca tcatcattga
     3661 ccgcgcggac cggtttggcc tgagcgatct ttatcaactg cgcggccggg ttggccgcta
     3721 caagcaccag gcctacgcgt atctgctgct gccgcgtcac gggaacctcc tcaccgatgt
     3781 ccgcaagcgt ctgagcgcga tcaagcagtt ctctacgctc ggcagcgggt tcaaaatcgc
     3841 catgcgcgac ctggaaatcc gcggcgcagg caacctgctc ggcgcggagc aaagcggcca
     3901 tatcaccgcg gtcgggttcg agctctactg ccaactgctc aagcagagcg tcgcgtcgct
     3961 aaaaggcgag aaggtcaagc cgcgggtgga ggtgcgggtg aacttggact tcctggcgct
     4021 cagcccgggg gaagagagtc caaagtctaa agtccaaagt ccaaagtcgg ggggcgggga
     4081 gccggagtcg gcatcacgca tcacgcatca cgcatc
//
LOCUS       DATCGS010000186         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_611407, whole genome shotgun sequence.
ACCESSION   DATCGS010000186 DATCGS010000000
VERSION     DATCGS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611407"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..720)
                     /locus_tag="VIL39_03740"
     CDS             complement(187..720)
                     /locus_tag="VIL39_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY5480567.1"
                     /translation="MKNIVYFDLETQKSAEEVGGWDNIIRMGMSIGVTYSSAKGAYKI
                     YGEKQVNDLITELQRADLVVGFNNLRFDYEVLHNYTSFDLRQLPTLDMMVELQNQLKH
                     RLSLDSIATATFGVEKTAEGMQAIQWFKEGKLLEIAEYCCYDVKITRMVHEFGVQHRQ
                     LHYHNRFGRKMTVPVKW"
     gene            859..1422
                     /locus_tag="VIL39_03745"
     CDS             859..1422
                     /locus_tag="VIL39_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5480568.1"
                     /translation="MKWRWLCLILILLLVGGPATYWWWQGRLERSQDGPIRAAARRYG
                     VEPALIKAIVWRESRFHPRVRGRAEEIGLMQLQEEAAREWASAEHLQAFEHEQCFDPG
                     TNTLAGTWYLRKLLRRYAQTDNPLPYALADYNAGRGNVLKWNQGAAATNSAAFTAQIG
                     FPGTQDYVRSVMRRYAHYRPVFRPDAP"
     gene            complement(1434..2162)
                     /locus_tag="VIL39_03750"
     CDS             complement(1434..2162)
                     /locus_tag="VIL39_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5480569.1"
                     /translation="MSDPTPKTESPRGAARRRRSGGVVVPHAPYWHQRLGARLVHLLI
                     RSVSATLRNQWIDRSGYFDNGPADPAIYCVWHNRLALCLITYFDYVKKHNQTPGMAAL
                     VSASKDGGFLASILECFGVQPVRGSSSRRGPQALLELTGWAERGYDLAITPDGPRGPR
                     YVVQEGVIALAQVTGLPIIPASHYVRWKIQAKSWDRFQIPLPFSHSELILGKPVRVPR
                     EASDAEREALRQQLERTLQEISRD"
     gene            complement(2168..>2563)
                     /gene="ruvA"
                     /locus_tag="VIL39_03755"
     CDS             complement(2168..>2563)
                     /gene="ruvA"
                     /locus_tag="VIL39_03755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5480570.1"
                     /translation="LFRLLINTVSGIGPKTALNLLSGISVTAFRGAVANGDIKALSQI
                     SGVGKKTAERIVVELKDKVGAASAWEASSAQRALSPEDQKVNDAVLALMALGFKQVEV
                     HDLVRKVQSSLGPQATVEDLVRACLRKGA"
BASE COUNT          472 a          797 c          796 g          498 t
ORIGIN      
        1 aagaccgaga aagtgaagct cgcctgggcc aagcccgcgc cgcagcaggt gtggctcagc
       61 gacaccagcg agcagccgcg cgataaggtt ggctcatctg tcgaagtgcc cggttggggc
      121 atcgtcaccc tgcgcgcgga ataggcgggt gctcgcgaca gagtcgccaa ggctctgcac
      181 ctctcgctac cacttcaccg gcacagtcat ctttctcccg aagcggttgt ggtaatgaag
      241 ctgccggtgt tgcaccccga attcatgcac catgcgggtg atcttcacgt cgtagcagca
      301 atactcggcg atttcgagca gcttgccttc cttgaaccat tggatggcct gcataccctc
      361 ggcggtcttc tccacgccga acgtcgccgt ggcgatggaa tccagggaga gccggtgctt
      421 caactggttc tgcagctcga ccatcatgtc cagcgtcggc aactggcgca ggtcaaagga
      481 ggtgtagttg tgcagcactt cgtaatcgaa ccgcagattg ttaaacccca ccaccaagtc
      541 cgcgcgctgc aattccgtga tgaggtcgtt gacctgcttc tcgccgtaga tcttgtaggc
      601 gcctttcgcc gagctgtagg tcacgccgat gctcatgccc atgcggatga tgttgtccca
      661 gccgccgact tcttcggcgg acttctgcgt ttccaggtcg aaatagacaa tattcttcat
      721 cagcgcttca ggttgcggcg gaagagcaca cccgccacgc cgccactgta tcccggatgg
      781 ggaaggaggc aaaagaaaat ccggcagacg acgatcgagc ctctcgacgc ggcgttcggg
      841 gtgcggtaaa aagaccgcgt gaaatggcgt tggctgtgtt tgattctgat cctgctcctc
      901 gtgggcggcc ccgctacgta ctggtggtgg caaggccgtc tcgaacgaag ccaggacggc
      961 ccgattcgcg ccgcggcaag acgctatgga gtggaacccg ccctgatcaa agccattgtc
     1021 tggcgcgaaa gccgtttcca tccccgggtg cgggggcgcg ccgaagaaat cggtttgatg
     1081 cagcttcagg aggaggccgc gcgggaatgg gccagcgccg aacacctgca agcgttcgag
     1141 cacgagcagt gcttcgatcc cggcaccaac acgctggcag gcacgtggta tctgagaaaa
     1201 ctgctgcggc ggtatgcgca gacggataat ccgctgccct acgcgctggc ggattacaac
     1261 gcgggccgcg gcaacgtgct taagtggaac cagggcgcgg cggcgacgaa tagcgccgcg
     1321 tttaccgcgc aaatcggctt ccccggcacc caggattatg tgaggtcagt gatgcgccgt
     1381 tacgctcact accggccggt tttccgcccg gacgcgccgt gacaccggtt cactcaatcc
     1441 cgtgagatct cctgcaatgt tcgttccaat tgctgccgca aggcttcgcg ttctgcgtcc
     1501 gaagcttcgc gtgggacccg gaccggcttt ccgagaatca gttcactgtg cgagaagggg
     1561 agggggattt gaaaccggtc ccagctcttc gcttggatct tccagcggac atagtgcgag
     1621 gccgggatga tcgggaggcc ggtgacctgc gccagcgcga tgacgccttc ctgcaccacg
     1681 tagcgcggtc cacgcggtcc gtcgggcgtg atggccagat cgtagccgcg ttccgcccag
     1741 ccggtgagtt ccaatagggc ttgcgggccg cgccggctgg aggagccgcg caccggctgc
     1801 actccgaaac attcaaggat gctggccaga aagccgccgt ctttgctcgc gctcaccagc
     1861 gcggccatgc cgggcgtttg gttgtgcttc ttaacgtagt cgaaatacgt tattaggcac
     1921 agcgccagcc ggttgtgcca cacgcagtaa atcgccgggt cggcggggcc attgtcgaaa
     1981 tagccagagc gatcgatcca ttggttgcgc aacgtcgccg atacgctccg gatcagtagg
     2041 tggaccagcc gcgcccccag ccgctggtgc cagtacggcg cgtggggcac gacgaccccg
     2101 cctgacctgc gccttcgggc tgcgccccga ggagattcgg ttttgggtgt tggatcgctc
     2161 acaatcatta cgctcccttt ctcaggcagg cgcggacaag gtcttccacg gtggcctggg
     2221 gacccagcga gctttgcact ttgcggacca aatcatgcac ctcgacctgt ttgaagccca
     2281 gcgccatcaa agccagcacg gcgtcgttga ccttctggtc ttcgggcgag agggcgcgtt
     2341 gcgcgctcga agcctcccag gcgctggcgg cgccgacctt gtccttgagc tcaaccacaa
     2401 ttcgctcggc cgtcttcttg cccacgcccg atatttgtga cagcgcctta atgtccccat
     2461 tggccaccgc gccgcgaaag gccgtcacgc tgatcccgct cagcaaattg agcgcggtct
     2521 ttgggccgat gccgctgacc gtgttgatga gcagacggaa caa
//
LOCUS       DATCGS010000187         4553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_512592, whole genome shotgun sequence.
ACCESSION   DATCGS010000187 DATCGS010000000
VERSION     DATCGS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512592"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1848)
                     /locus_tag="VIL39_03760"
     CDS             complement(148..1848)
                     /locus_tag="VIL39_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5480571.1"
                     /translation="MTFLPIVARELRIAARRPGTFWVRSGAALAILIIGTWFFLMNQG
                     QPTQVIALGLFSILTGSAVLYCLLSGVWFTADCLSEEKREGTLGLLFLTDLKGYDVVF
                     GKLAATSLNGFYAVLATVPILALPLLLGGVTGGEFGRMALVVVNTLFFSLALGICVSA
                     MSRSPRKAMAMTFLLILLFTAMLPACGAWRSVAIKAPQVAKAWLMPSTGFSYCLALDV
                     FYKTRAPEFWWSVAVIHGLGWIFLLVASFIAPRSWQDQPAGAQTLRWRERWRWWSYGN
                     VAERAAFRRRLLDQNAYFWLAARARLKPAYVWAVLGLVACGWVWGLARSGRDWLHEMT
                     YVLTGLLLNLLIKVWFALEAGRPLAEDRRQGAFELLLSTPLTENDILRGQLLALKRQF
                     RGPVVVVLLVFFLFAMAASSDALSQQEPQDQILWVLFWAAAMVMLVADLMALYWVGMW
                     AGLTARNPTRAAAANLGRILALPWIAVFIGILVASIAWPNTDETQGQKLFLGLWFGLG
                     LAADLGFGAWARHRLLTEFRLAATRRYELLPGFWKRLLSGGGPASVAVPQVQGVADGE
                     "
     gene            complement(1906..3489)
                     /locus_tag="VIL39_03765"
     CDS             complement(1906..3489)
                     /locus_tag="VIL39_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480572.1"
                     /translation="MTILPVIARELRASARQPFTYYLRVLGVTALLLASLLFGLHFGF
                     EPALGRQLFAALHGTLFGAIWLLVPMLTADCISRERREGTLGLLFLTPLTGTDIVVAK
                     GLVHGLRAMTLWLAVLPVLTVPFLMGGVSWRQAAMSVMVNANAMCWALAAGLLASAWS
                     KAWSRALLGAALLACYFLLLLCAGAGWLLLHAVSASRPGQFPISWDQSAAAGLFVLVN
                     ISEDWSRYFRWGSPSQMIWALGQTTLVSFLMLVVAVLVAGAKTRRSWQEEPPSRRQVW
                     VQRTFCTPVLWLGFFHRWMRRRLERNPIGWLEQRTWSGRLVTWGWLAVLVSIYSAVFT
                     DRQFFVNANGLQETMAWLLAGSMAMSAAGSFRRERDTGVLELLLVSPLGENQIISGRL
                     GGLWSQFLPAAGLLLSVWAYCSTFLSNGATVGMGLFFLAAFLTVPVFGLYFSLLCRNF
                     IIAFLATLAVGLLLPLVLPQALRALWWTVFNPAEVFSWEPRPSLGAVVCQILLAVVCW
                     NRLFVRLKARAFPLERTEG"
     gene            complement(3521..>4553)
                     /locus_tag="VIL39_03770"
     CDS             complement(3521..>4553)
                     /locus_tag="VIL39_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480573.1"
                     /translation="ESPSPQETSGTTLLSNAAAPEDGRTPTVGTECTLPGEREAEPAW
                     PSRRPLGDENPIVWLLQRQRGIPALLWAGALVSLTHYGLFTIGLRFLSPGLYSSVTWP
                     LSLASTALEGALFAWAASRFFVEARRTGELELLLATPLGASEIVSAQWDVLKRLLRWP
                     MLVLLAPTLLQSAVLIATMPLQPAVLIAAMPTARSFGAGSSFGVHFAISCVVRLVDTF
                     FGVGALCWLGLWFGFRAGGQGRAIAWTISLAGGLPFLISLLCSILLPMLTRSAGAGRS
                     SSYWIIMWLPQAITLVLYLGLIRLARQRLAGELAGAEVMEFDLRQFISSAARDTLSGF
                     NKARHWTPS"
BASE COUNT         1023 a         1526 c         1320 g          684 t
ORIGIN      
        1 tggtcaaccg gggacgaggc cgtccccgcc cctgcctttt gcgcttcatg aattggccct
       61 gcgttaacta agtgccattc gagtccggct ctcatctgtg tccatctgtg ttcatctgtg
      121 gttcacttct gcttcccgac cacggcgcta ctctccatcc gccaccccct gcacctgcgg
      181 caccgccacg ctcgccggcc cgcctccgct cagcaagcgc ttccagaatc ccgggagcag
      241 ttcatatctg cgagtcgcgg cgaggcggaa ctcggtcagc aatctgtgtc ttgcccaggc
      301 gccaaaaccg aggtcggctg ccaaccccaa cccgaaccac aagcccagga aaagcttctg
      361 cccctgcgtc tcatccgtgt tcggccaggc gattgatgcc accaggattc caatgaacac
      421 ggcaatccac ggcagggcca ggatgcgccc caggtttgcc gcggcggcgc gggtgggatt
      481 tcttgccgta agccccgccc acatcccgac ccagtagaga gccattaggt ccgcgaccag
      541 catcaccatg gccgcagccc agaacaggac ccataggatc tggtcctgcg gctcctgctg
      601 cgacagggca tcggacgagg cggccatagc gaaaaggaag aataccagca ggaccaccac
      661 caccggcccg cggaactgac gtttcaacgc gagcagttgg ccgcgcagaa tatcgttctc
      721 tgtcaacggc gttgacagca gcagctcgaa tgcgccttgt cggcggtctt cggccaacgg
      781 ccgaccggct tccagggcga accagacctt gatgagcagg ttcaggagca agccggtcaa
      841 cacgtaggtc atctcgtgca gccagtcgcg gcctgaccgc gccagccccc agacccaccc
      901 gcaggccacc agaccaagca ccgcccaaac ataagccggc ttcaatcgcg cgcgcgctgc
      961 cagccagaaa taggcgttct gatccagcaa gcgtctgcga aacgcggcgc gctccgccac
     1021 gttgccgtaa ctccaccagc gccagcgttc acgccagcgc agcgtttgcg cgcccgccgg
     1081 ctggtcctgc cacgagcgag gcgcaatgaa acttgccacg agcaggaaaa tccaacctag
     1141 cccgtgaata accgccaccg accaccagaa ttcaggcgcg cgggtcttgt aaaacacgtc
     1201 gagcgccagg cagtagctga agcccgtgct cggcatcagc cacgcctttg ccacctgcgg
     1261 agccttgatg gcaacggacc gccatgcacc gcaagccggc agcatagcgg tgaaaagcag
     1321 gatgagcaaa aacgtcatag ccatcgcctt gcgcggcgat cggctcatgg ccgagacgca
     1381 tatcccgagc gcaagggaaa agaagagggt gttgaccacc accagcgcca tgcgcccgaa
     1441 ttcgcccccc gtcacccctc ccaacaagag cggcagggcc aggatcggca ccgtcgccag
     1501 gacggcgtag aagccgttca gcgaagtggc cgcgagtttg ccgaacacca cgtcgtagcc
     1561 tttgaggtcg gtcaggaata acaggcccag cgtcccttcg cgcttctctt cgctcaggca
     1621 gtcggccgtg aaccacacgc cgctcagcag gcagtacaac accgcgctgc cagtcaggat
     1681 cgagaacaag cccagggcga tcacctgtgt cggctggccc tgattcatca ggaagaacca
     1741 cgtgccaatg atgaggatcg ccagcgccgc gccgctgcgc acccaaaagg tgccgggccg
     1801 ccgcgccgca atccgcaatt cccgtgcaac aatgggcagg aaggtcatcg gcttgcttta
     1861 ctgagtttgc ttactcgcgt ttcggccatt cggtgttcgg atttgttatc cctcagttct
     1921 ttccaaagga aaagcccgtg ccttcagccg gacaaagagc cggttccagc agaccacggc
     1981 gaggagaatc tggcaaacaa ccgctcctaa ggacgggcgc ggttcccatg agaagacctc
     2041 ggccgggttg aagacagtcc accacaacgc ccgaagggcc tgggggagca ccaggggcaa
     2101 cagcagcccc accgcgagcg tggccaggaa ggcgatgatg aaattgcggc aaagcaagga
     2161 aaagtagagc ccgaacaccg gcacagtcag aaacgccgcg agaaagaaca aacccatccc
     2221 gacagtcgcg ccattcgaca ggaaagttga acagtacgcc cagactgaca ggagcagacc
     2281 ggcagccgga aggaactggc tccataggcc gccaagccgc cctgaaatga tttggttctc
     2341 gcccagcggt gaaaccagca gcagttcgag cacgccggtg tcgcgctcac gccggaagct
     2401 tcccgccgcg ctcatcgcca tgctgccggc caacagccag gccatagtct cttgcagacc
     2461 gttggcgttc acaaagaact gccggtccgt gaacaccgcg ctatagatcg agaccagtac
     2521 cgccagccag ccccaggtca ccagccttcc gctccaggtg cgctgttcca gccagccgat
     2581 gggattgcgc tccagtctcc ggcgcatcca gcggtgaaag aagccaagcc aaaggaccgg
     2641 ggtgcagaag gtccgttgca cccaaacctg acgccgcgag ggaggctcct cttgccagga
     2701 gcgccgggtc tttgcgcccg ctaccaatac cgcgacaacc aacataagga aggacacgag
     2761 tgtcgtttgt ccgagcgccc agatcatttg gctgggagag ccccaccgga agtacctgga
     2821 ccagtcttcg cttatgttta caagaacaaa gagcccggca gccgccgact ggtcccagct
     2881 tatcgggaac tgaccaggtc ggctggcgct aacggcatgc agcagcagcc aaccggctcc
     2941 tgcgcacaaa agcagcagga agtaacaggc caggagggcc gctcccagga gcgcccgcga
     3001 ccacgctttg ctccacgccg aggccagcaa accggccgcc agcgcccaac acatcgcatt
     3061 ggcattgacc atcactgaca ttgcggcctg cctccaactc acaccgccca tcaagaaagg
     3121 gaccgttaac accggcagca ccgccagcca taacgtcatc gctctcaatc catggaccag
     3181 tcctttggcc acgacgatgt ccgtccccgt caagggtgtc aggaatagca gccccagggt
     3241 tccttcacgc cgctcccggc tgatgcaatc agcggtaagc atcggcacca gcaaccaaat
     3301 tgcgccgaag agtgtgccat gaagcgccgc aaacaactgg cggcccaaag cgggctcgaa
     3361 cccaaagtgc aagccaaaca gcaggctcgc cagcaacagt gccgtcaccc ccagcacccg
     3421 caggtaataa gtgaagggct gccgcgccga ggcgcgcagt tcacgggcga tgacgggcag
     3481 gatcgtcata ggggtcggtg actggtgatc tcagcttcgt tcatgacggg gtccagtggc
     3541 gcgctttatt gaagcctgac agggtgtcgc gcgcagcgga tgaaatgaac tgtcgcaagt
     3601 cgaactccat gacttcggcc ccggccaact cgcccgccaa acgctgccgg gccagccgaa
     3661 tcagcccgag gtaaagcaca agggtaatgg cttgcggcag ccacattatg atccagtaag
     3721 acgatgatct cccagcgcca gccgaacggg tcagcatcgg gagcaggatc gagcacagaa
     3781 ggctgatcag aaatggcagg cccccggcca ggctgatcgt ccaagcgatc gctcggcctt
     3841 gcccacccgc cctgaagccg aaccacagcc ccagccagca gagcgctcca acgccaaaga
     3901 aggtgtccac taatcggact acacaggaaa tggcgaagtg cacgccgaac gaactgcctg
     3961 caccgaacga gcgagcggtt ggcattgccg ctataagcac tgcgggctgc agtggcattg
     4021 tggctataag caccgcggac tgcaggagag tcggcgcgag gagcaccagc attggccagc
     4081 gaagcaggcg cttcaatacg tcccattgcg cggaaacaat ctcgctcgcc ccgagcggcg
     4141 tggccaacag aagttcgagc tcgcccgtgc gccgggcttc gacgaagaaa cggcttgccg
     4201 cccaagcaaa caaagcgccc tcgagcgccg tagaggccag cgacaaaggc cacgtcactg
     4261 acgagtataa gccagggctc aggaagcgta atccgatcgt gaacaatcca tagtgagtga
     4321 ggctgaccaa cgcccccgcc cagaggagcg ctggtatgcc gcgctgccgt tgcaagagcc
     4381 agacgattgg attctcgtcc cccaagggcc gccgcgaagg ccatgccggt tccgcttcgc
     4441 gctccccagg caaggtgcat tctgtaccta ccgtcggagt gcgcccgtcc tcgggcgcag
     4501 cggcgttcga cagtagtgtg gtcccggacg tttcctgcgg gctgggcgat tcg
//
LOCUS       DATCGS010000188         6885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_362355, whole genome shotgun sequence.
ACCESSION   DATCGS010000188 DATCGS010000000
VERSION     DATCGS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362355"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..667)
                     /locus_tag="VIL39_03775"
     CDS             complement(203..667)
                     /locus_tag="VIL39_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480574.1"
                     /translation="MRRWFICLLLAGGLAVAWSAQAAAGRVIKVLPQFLDLKGRNSLS
                     PSLYERDTYQARLRDHTNLCSGIRFNVQWKAKGQATAPLKLRIELRGVAHGDFPKQLQ
                     LEKSVEPGGWFSHWTEVNLLGEEYRSFGQITAWRATLWEGPLVLGEQKSFLW"
     gene            791..1060
                     /gene="rpmB"
                     /locus_tag="VIL39_03780"
     CDS             791..1060
                     /gene="rpmB"
                     /locus_tag="VIL39_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY5480575.1"
                     /translation="MARICELTGKRPIKGSIIWRSGKSKKSGGIGTHVTAITKRRFFP
                     NLQRVKAVVNGEVRYVRVSVKAIKKGLVVKAPKRTWKKEAEAAKV"
     gene            complement(1140..2888)
                     /locus_tag="VIL39_03785"
     CDS             complement(1140..2888)
                     /locus_tag="VIL39_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5480576.1"
                     /translation="MNESQGTTRKEAGDLLLEHGKLTEQQLEQVRRRQRRLNLPQHRA
                     IVDLNFASEEDTWRALAAANHLEFVDPVGLDLKREILELVPIKLIFHYHLLPLGVEDD
                     CITLAFSEPPSQMEQGNLRLLLSKRFKIVLATPSSIHAVIKKNYGLGAETIQKLREDR
                     GGTEINQEIVFDVQGKDTDSALEATVSAFVDQILQEALRLRATDIHLEPYINSIRLRY
                     RVDGILETVPVPADMRHLHPAVISRLKIMAGLNIAEKRLPHDGRIAMKTGDEEYDLRV
                     SIVPTKYGEAICLRILGRQSLYLDLGQLGMEPNQEAVLAQLTALPQGMVLLTGPTGSG
                     KTTTLYTALAQANDEGRKVITFEDPIEYQLEGTVQIQVREQIGLTFAAGLRSVLRHDP
                     DVVLVGEIRDYETAEIAVRAAQTGHLVFSTLHTNDSVSAVTRLLEMKIESYLIASSLV
                     CSISQRLARRICRHCTEADLAIPDSVREEMAAALSLPAEQVKAWKGRGCVECNHKGHR
                     GRVAIYEFFLLNEAIVDLIQPGLRTGELRAAARKLGWRSLREMAWLKVQKGLIPISEQ
                     ERWTKVIDPAALLAKS"
     gene            complement(2885..3403)
                     /locus_tag="VIL39_03790"
     CDS             complement(2885..3403)
                     /locus_tag="VIL39_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480577.1"
                     /translation="MPPIVKRALRNRWFVTAVHAGLWFLLYLALAGLRGKAPDLRNSD
                     GATSPPHSSVPVAGLERLFSSGIWPKSLVETNRLDPFVTHYFIPPVAPPPTTRKIEVT
                     YLGFYQTGGGPKNTIFKLGDAFQVAPIGARIATNLFIADATMQTLTLTNLAAQTNVLS
                     LNTNKEIIVPIQ"
     gene            complement(3410..4231)
                     /locus_tag="VIL39_03795"
     CDS             complement(3410..4231)
                     /locus_tag="VIL39_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480578.1"
                     /translation="MKIPFGEYQRRGLIPLIGLALAAYYLFVMLPLTHKAANLNGPLQ
                     KAWHNLSLSLDQTNVNAIDFLNITNQLSETRQALLILENAKKQATSRLQLGPTVRARM
                     HRDFQLVDYENERSRQLDELSNLAKQRQAAVDPAVFAGFPEHTAGVKQPALLWASLSL
                     TESLLRTALQCKVAAIQSLEVPPVLTNAPPTNSTERLAEIPLQVELTGSTASVAMLIQ
                     SLPLRAEEIRAAGLPEAEADKPALFIDRLIIQKQTPDKTDEVHVWLRAVGFVVRE"
     gene            complement(4228..6090)
                     /locus_tag="VIL39_03800"
     CDS             complement(4228..6090)
                     /locus_tag="VIL39_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480579.1"
                     /translation="MLKLDRMRPPVRRVLALDAGSRCIKLLLAESDFGRLHILKEELI
                     DLQAEGLVSAEEIKAHLQTSLDEWGRPPLALILPQHLSISQVINLPLAPESEVEKLIQ
                     DETIKLGGVSESRIVYDFVRTETAAKNRQQFWVTLSQEGEIRERILRLGVEQEDLCEV
                     TTTANALIAAYRATSPLSSRAILVHLGAQTTVVVVLLAGQGAFATSFQMGGDFFTRSL
                     ARLRSSSEETAESLKHAKNLLTGPEAAPDFAAVVDGWVAELKRQLNEWFEHNPGLAPD
                     AASFELVASGGGFDQPGLLDYLKAQAGLNLKPWPKGSQPDAVLPSKRFEVAFGAALQA
                     LGYSAQPVSLLPDDYRVAWRKRLNLQRLEMASLGLAVICLLIFAFGTWHQVSLINRQQ
                     ALLVKVQAGQEAVEANDLLSAELAAEYEGFRPLFASQQNTLDTLKTLALLQESRSNRS
                     FWYVLLADQQSYFSQSPGGSPTNRLSRTNVFAAPASRSRSASSDFPSASATATNLSPA
                     KPGFIAELCIPEDADGARRVRRQLVDELKEQQLFSKVDLLSDDLRRNLANPRVIIPDR
                     DFVLALDFAATEFQQPLLLKRLLPGGASRATLRRAARTAWASPEGEENPAPIAP"
     gene            complement(6128..>6885)
                     /locus_tag="VIL39_03805"
     CDS             complement(6128..>6885)
                     /locus_tag="VIL39_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY5480580.1"
                     /translation="QNFPSAINPSGAVNPAVGGGISQVFTNVLGAGDLTATISALEQS
                     GESQTLSAPRLTVLNNRPASISDGQVHYYYEEYSVSSTVQQYYTASSIYPSGKPTKIT
                     AGAELNVLASVSGDGQSILLALNPKVNTQVLLETYTTISQYTAADTKPNTFDIKLPTY
                     RTQEISTRVAVKSGETVVMGGVLAREKSIYVESVPVLGDIPILGALFRRRTEQDTPRY
                     LLVFVTATIVKDSGEFLVYDDEQSATNSPAIAR"
BASE COUNT         1315 a         2067 c         2171 g         1332 t
ORIGIN      
        1 cgaagctaaa cgccccaaca ggtcgtgtca acgtatcgcg ccacatatcg cggacgggac
       61 atggtagcgg ctgagcgacg gagaacggcg acttcagcga agcgccggaa tgctacgaac
      121 aggccctgca cacgcaggaa acgcggcacc ccttgagcag cccacctgaa cgcgactgcc
      181 cgggctggaa acgccgcggc gcctaccaaa ggaacgactt ctgttccccg agaaccagcg
      241 ggccctccca cagagtcgcg cgccacgcag taatctggcc gaagctccta tattcctcac
      301 cgagcaggtt gacttcggtc caatgactga accagccgcc gggctccacg gacttttcga
      361 gctgcagttg tttgggaaag tcgccatgtg cgacgccgcg cagttcaatc cgcagtttaa
      421 gcggggcggt ggcctggccc ttggccttcc actgcacgtt gaaccgaatc cctgaacata
      481 agttcgtatg atcgcgcagg cgagcctggt aggtgtcgcg ctcgtaaagg cttggggaga
      541 gcgaattgcg tcctttcagg tcgagaaact gcggcaagac tttgatcacc cgacccgcgg
      601 ccgcctgggc cgaccaagcc accgccagcc caccggccag caacaaacaa atgaaccacc
      661 gtcgcatgaa tgacaaatag ccgccccagg cgcccgactc aaggctgatt tcgccaaaag
      721 gcagatacag cttgcaaaga cgaggacacc tgctattgtt tgtgttcttt aatgttcaaa
      781 caggatttgt atggcgagaa tttgtgaact gacaggcaaa cgcccgatca agggcagcat
      841 catctggcgc agcggcaagt cgaagaaaag cggcggtatc ggcacgcacg ttacggccat
      901 caccaagcgc cggtttttcc caaacctgca gcgtgtcaag gccgtcgtta acggcgaggt
      961 gcgctatgtc cgcgtttcgg tcaaagctat aaagaaggga ttggtcgtca aagcgcccaa
     1021 acgcacctgg aagaaggaag cagaggccgc caaggtctga tccaaccaga atagagcttt
     1081 cacgaaagag gatggcgttc aaccgagcgc catcctcttt ttatctcgca gattgatgtt
     1141 tagctcttgg ccagcagcgc ggcggggtcg attaccttgg tccaccgttc ctgctcggaa
     1201 atcgggataa gtcctttttg gactttcagc caggccatct cgcgcaagga tcgccagccc
     1261 agcttccgcg ccgccgcgcg gagctcgccg gtccgcaagc cgggctggat gaggtccaca
     1321 atggcttcgt tcaggaggaa gaactcatag atggcgaccc ggccgcggtg gcctttgtga
     1381 ttgcattcga cgcagccgcg gcctttccag gctttgacct gttcggcggg gagcgaaagc
     1441 gcagcggcca tttcttcgcg gaccgagtcc gggatggcca ggtctgcctc ggtgcagtgg
     1501 cggcaaatgc ggcgggccaa ccgttgcgag atgctgcaaa ccagcgagga agcgatgagg
     1561 taagactcaa tcttcatctc cagcaggcgc gtgacggcac tgacgctgtc gttggtgtgc
     1621 agggtcgaga aaacgaggtg gccggtttgg gcggcgcgga cagcgatttc ggcggtttca
     1681 tagtcccgaa tctcgccgac gagcacgaca tccgggtcgt ggcgcagcac cgaacgcagg
     1741 ccggcggcga acgtcaggcc gatttgctcg cggacctgga tttgcaccgt gccttcgagc
     1801 tgatactcga tggggtcctc gaaggtgatg accttgcggc cttcatcgtt cgcctgagcc
     1861 agcgcggtgt aaagggtggt ggtcttgccg ctgccggtcg gcccggtcag caggaccatg
     1921 ccttgcggca aggcggtcaa ctgagccagc acggcctcct ggttcggctc catgccgagc
     1981 tggccgaggt cgaggtaaag gctctggcgg cctagaatcc gcaggcatat cgcttccccg
     2041 tacttggtgg gcacaatcga aacccgcagg tcatattctt cgtcgcccgt cttcatggcg
     2101 atgcggccgt cgtggggcag ccgtttttcg gcgatgttca gccccgccat gattttgagc
     2161 cgcgagatca ccgccgggtg caagtggcgc atgtccgccg gcaccggcac cgtttccagg
     2221 atcccgtcca cgcggtagcg caggcggatg gaattgatgt agggttcgag atggatgtcc
     2281 gtcgcgcgga ggcggagggc ttcctgcaga atctggtcca caaaagccga gacggtggct
     2341 tcgagcgcgg aatcggtatc tttgccctgc acgtcgaata cgatctcctg gttgatctcc
     2401 gtcccaccgc ggtcctcgcg cagtttctgg atcgtctccg cgcccaggcc ataattcttc
     2461 ttgatgacgg catggattga gctgggagtg gcgaggacga tcttgaagcg tttgctcaac
     2521 aggaggcgca ggttgccctg ctccatctgg ctcggcggct cgctgaaggc gagcgttatg
     2581 cagtcgtcct cgacgcccaa cggcagcaaa tggtagtgaa aaatgagctt gatgggcacc
     2641 agctccagaa tctcgcgctt aaggtctagc cccactgggt ccacgaactc cagatgattg
     2701 gcggcggcca aggcgcgcca ggtgtcctct tccgacgcga agttcaggtc cacgatcgcg
     2761 cgatgctgcg gcaggttcag ccgccgttgg cggcgccgga cctgctcgag ctgctgctcc
     2821 gtaagcttgc cgtgctccag caacaggtcg ccggcttcct tacgggttgt gccctggctc
     2881 tcgttcattg aattggaacg atgatttcct tattcgtgtt cagggacaga acattggtct
     2941 gcgccgccag gttcgtcaat gtcagcgttt gcatcgtcgc gtcggcaatg aagaggttgg
     3001 tggcgatcct ggccccaatg ggggccacct ggaacgcatc gcccagtttg aagattgtgt
     3061 tcttgggacc accgccagtc tggtagaaac ccaggtaggt cacttcgatc ttgcgggtgg
     3121 ttggtggcgg cgcgaccggc ggaatgaagt aatgggtaac gaagggatcc agccggttcg
     3181 tctcgaccag cgatttaggc cagatccccg acgaaaagag gcgctccagc ccggcgaccg
     3241 gcacggaact gtgcggcggg ctggtggccc cgtcggagtt ccgcaaatcg ggggccttgc
     3301 cgcgaagacc cgccaacgcc aggtaaagca ggaaccacag gccggcatgc acggcggtca
     3361 caaaccagcg gtttctcaag gctcgtttta caatgggcgg catattccct cactcccgca
     3421 cgacaaatcc gacggcgcgc agccacacgt gcacctcgtc cgtcttgtct ggcgtttgct
     3481 tctgtatgat cagccggtca atgaatagcg cgggtttgtc ggcctccgcc tcgggcagcc
     3541 cggccgcgcg aatttcctcg gcgcgcaggg gcagcgactg gatgagcatg gcgacgctgg
     3601 ccgtcgagcc ggttaattca acctgcaatg gtatttcagc caggcgctcg gtgctgttgg
     3661 tgggcggggc gttggtgagc accggcggca cctccagaga ttggatggcg gcgactttgc
     3721 attggagagc ggtccggagc aggctctcgg tgagcgagag cgacgcccag agcagggccg
     3781 gttgcttcac gccggccgta tgctcgggga agccggcgaa gacggcgggg tcaacggcgg
     3841 cctgccgttg tttggcgagg ttgctcaact catccaactg ccggctgcgc tcattttcgt
     3901 aatccacaag ctggaagtcc ctgtgcatcc gggcgcgcac cgtcgggccg agttgcagcc
     3961 ggctcgtggc ctgcttcttg gcgttctcca ggatgagcag ggcctggcgg gtttcactga
     4021 gctggttggt aatgttcagg aaatcaatgg cgttaacgtt ggtctggtcc agcgagagcg
     4081 agaggttgtg ccaggccttt tgcagcggcc cgttcaggtt cgcggccttg tgcgtcaggg
     4141 gcagcatcac gaacaggtaa taggccgcca gggccagccc gataagcggg atgaggccac
     4201 gtcgctgata ttcgccaaag ggaatcttca cggcgctatg ggagcgggat tctcctcacc
     4261 ttccggcgac gcccacgccg tgcgcgccgc gcgtcggaga gtcgcccggg acgctccccc
     4321 gggcagcagc cgcttcagca acagcggctg ctgaaactcc gttgccgcaa aatccagcgc
     4381 gagcacgaag tcccggtcgg gaataatgac ccgcggattg gccaggttcc ggcgcaaatc
     4441 gtcggagagc aaatccactt tcgagaacaa ctgctgctcc tttagttcat ccaccagttg
     4501 gcggcgcacc cggcgggcgc cgtccgcgtc ttccgggatg caaagctcgg cgatgaagcc
     4561 gggcttggct ggtgagaggt tggtggcggt cgccgaggcc gacgggaaat ccgaggaagc
     4621 actgcgggac cggctggccg gcgcggcaaa cacgttcgtc cgggaaagtc tattggtcgg
     4681 agatcccccc ggcgactgac tgaagtagct ttgctggtca gccagcagga cgtaccagaa
     4741 gctgcgattg ctgcgggact cctggaggag cgcgagcgtt ttcagcgtgt cgagcgtgtt
     4801 ctgttggctg gcgaacagcg ggcggaaacc ctcgtattcg gcggccaact cggcgctcag
     4861 caggtcgttg gcctccacgg cctcctggcc ggcctgaact ttcaccaaca aagcttgctg
     4921 gcggttgatc agcgacactt ggtgccaggt gccgaacgca aagatcagca ggcaaatcac
     4981 cgccagcccg aggctggcca tttccagccg ctggagattc agccgcttcc gccaggcgac
     5041 acggtaatcg tcgggcagca acgagaccgg ttgggcactg tagccaagcg cctgaagcgc
     5101 ggcgccgaaa gcgacttcga agcgttttga aggcaagacc gcatcgggct ggctgccctt
     5161 gggccagggc ttcaaattca atcctgcctg cgccttcagg taatcgagca aacccggctg
     5221 atcaaacccg ccaccgctgg cgaccaattc aaaggaggca gcgtccggcg ccaggccggg
     5281 attatgctcg aaccactcgt tcaactgccg cttcaattcc gccacccagc cgtccaccac
     5341 cgcggcaaag tccggcgccg cctccgggcc ggtcaaaaga ttcttggcgt gcttcaagga
     5401 ctccgccgtt tcttcagagg aacttcgaag ccgcgccagg gagcgagtga agaaatctcc
     5461 ccccatctgg aagctggtgg cgaaggctcc ctgacccgcc agcaggacca ccaccaccgt
     5521 cgtctgggct cccagatgca ctagaatggc gcgggacgac aggggactgg tggcgcggta
     5581 ggcggcaatc aaagcgttgg ctgtcgtggt aacctcgcac aggtcttcct gctcgacgcc
     5641 caaccgcaaa atgcgttccc gaatctcgcc ttcctgcgaa agagtcaccc agaactgctg
     5701 ccggttcttg gcggcagttt ctgtgcgaac gaagtcataa acgatacggc tttcgctgac
     5761 gccacccagt ttgatggttt cgtcctggat caacttctcc acttcgctct ccggcgcgag
     5821 tggcagattg atgacctgtg aaatggagag gtgttgcggc aggatcaggg ccagcggcgg
     5881 tcggccccat tcgtccaggc tggtttgcaa gtgcgccttg atctcctccg ccgaaaccag
     5941 gccctcggcc tgcaagtcga tcaattcctc cttgagaata tgcaggcgcc cgaagtcgct
     6001 ttccgccagc aacagcttga tgcagcggct gcccgcatca agcgccagca cccgccgcac
     6061 cggcggtcgc attcggtcaa gcttcaacat ggcgagggga cggtgactgg aagagcaatt
     6121 cccaacttca tcttgcaatc gcaggcgaat tagtggcgga ctgctcgtcg tcatagacga
     6181 ggaattcgcc actgtcctta acgatcgtgg cggtcacaaa aacgagcagg tatcgcggcg
     6241 tgtcctgttc cgtccggcgc cggaacagcg cgcccagaat ggggatgtct cccaggaccg
     6301 gcaccgactc gacatagatg gatttctccc gcgccagcac cccgcccatc accacggtct
     6361 cgcctgactt gacggcgacg cgcgtggaga tctcctgcgt gcgataggtt gggagtttga
     6421 tgtcgaaggt gttgggtttg gtgtccgccg cggtgtactg gctgatcgtg gtgtaggttt
     6481 ccagcagcac ctgggtattc accttggggt tcaaagccag caggatactc tggccatcgc
     6541 cgctgacgct ggccagcacg ttcaactctg cgccagcggt gatcttcgtc ggtttgccgc
     6601 tggggtagat tgaggaggcc gtgtagtatt gctgcacggt ggatgagacg ctatactcct
     6661 cgtaataata atggacctgg ccgtcgctga tgctcgccgg gcggttgttg agtactgtca
     6721 ggcgcggcgc gctcagcgtc tggctttccc cgctttgctc taatgcgctg atggtggcgg
     6781 tcaggtcccc cgcgcccagc acgttggtaa acacctgcga aatcccacca cccaccgccg
     6841 gattcaccgc gcctgaggga ttgatcgcac tggggaaatt ctggt
//
LOCUS       DATCGS010000189         2764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_413751, whole genome shotgun sequence.
ACCESSION   DATCGS010000189 DATCGS010000000
VERSION     DATCGS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413751"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VIL39_03810"
     CDS             <1..345
                     /locus_tag="VIL39_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEY5480581.1"
                     /translation="APKSDRLLDNYTHVDAMTLNIERACQAAIDMAMHLVAREHAGIP
                     QSSADAFILLGKAGLLQAEVVKEMISMTGFRNVAVHEYRELDMGVLRLIAVDRWKSLM
                     RLCEQFGMLIKP"
     gene            complement(522..1571)
                     /locus_tag="VIL39_03815"
     CDS             complement(522..1571)
                     /locus_tag="VIL39_03815"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY5480582.1"
                     /translation="MITTRGYAAQNATSSLTPFTFERREPGPHDVQIEILYCGICHSD
                     LHQVRNDWAGSMYPMVPGHEIVGRVTRVGAQVTKVKTDDYAGVGCMVDSCRRCPACNE
                     GLEQYCQEGCTFTYNSKERGSEQLTFGGYSERVVVEERFVVKIPGSLDLKAVAPLLCA
                     GITTYSPLRHWKVGKGQRIGVIGLGGLGHMGVKLAKALGAQVVMITTSPAKGKDAVRL
                     GADEVLVSKDSAAMAKQAGSFHFLLNTIPNPHDVNPYLALLKRDATMVLVGALTALEP
                     PLQGISLIGGRRCLAGSVIGGMPETQEMIDFCAAHEIVSDVEMVPIQSVNEAYERLLK
                     NDVRYRFVIDMASLK"
     gene            1996..2274
                     /locus_tag="VIL39_03820"
     CDS             1996..2274
                     /locus_tag="VIL39_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480583.1"
                     /translation="MIGTTQYLEAAKLISGTEYECGLWELEGRTMEWIAERSDPDNLA
                     QRQQSISAFFDGLKGDTYRAEQRPAVYFVLCLIVAVVSYSMVRKAFQK"
     gene            2367..>2764
                     /locus_tag="VIL39_03825"
     CDS             2367..>2764
                     /locus_tag="VIL39_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480584.1"
                     /translation="MAISRRLFLSRAATASAVAAFSSPLTSSCAAAQRSGEPEFHGVD
                     FHVHLDNSTLDKALELSRERGFKFGIVEHAGTKENQYPTLLSNDEDMRRYLAMLEGQP
                     VFKGIQAEWTDWMSCFSRQTLAQLDYVLTDA"
BASE COUNT          522 a          861 c          824 g          557 t
ORIGIN      
        1 gcccctaagt ccgacaggct gctagacaac tatacccatg ttgacgccat gacccttaat
       61 attgagcgtg cctgtcaggc tgccattgat atggccatgc accttgttgc ccgcgaacat
      121 gccggcattc cacagagcag cgccgacgcc tttatccttc tgggcaaagc cggcctgctt
      181 caggcggagg tcgttaaaga aatgatcagc atgacgggct tccggaatgt ggcggtgcat
      241 gaataccgcg agttggacat gggcgtgttg cgtttgatcg cggtggatcg ctggaaatca
      301 ctgatgcgcc tttgtgagca gttcggcatg ctcataaagc cctgacagac ttgctaccct
      361 cgcgcgccga aagccattcg gtgctgcggg cgcattggcg gctgcttcag cctcacggct
      421 gcaatagtcg aaattgttcg cgcaattcgg ttgcggtttt gccgcgctgc gctcctggcg
      481 ccgtcgcggc ccccaacccc tcgaaaccaa gctcggcccg cctacttcag cgaggccata
      541 tcaatcacga agcggtaacg gacatcgttc ttcagcagcc gctcatacgc ctcgttgacc
      601 gattggatcg gcaccatctc gacgtcgctg acgatctcat gggcggcgca gaaatcaatc
      661 atctcctggg tctccggcat gccgccgatg actgatccgg cgaggcaacg gcggccacca
      721 atcaggctga tgccctggag cggcggttcg agcgcggtca gcgccccgac cagcaccatc
      781 gtggcatcgc gtttcagcag cgccagataa gggttgacgt cgtgggggtt ggggatggtg
      841 ttgagcagga aatggaaact gccggcctgt ttggccatcg ccgccgaatc cttcgacacc
      901 agcacctcat cggcgccgag ccggaccgca tccttgccct tggccggcga cgtggtgatc
      961 atcaccacct gggcgccgag ggccttggcg agtttgacgc ccatgtggcc gaggccaccg
     1021 aggccaatca ccccgatcct ctggcctttg ccgaccttcc agtgacgcaa cggcgaatag
     1081 gtcgtgatgc cggcgcagag cagcggggcg accgccttca ggtcgaggct gccggggatc
     1141 ttcaccacga accgctcctc gaccacgacc cgctcggaat agccgccgaa ggtcaactgc
     1201 tcgctgccac gctctttgga gttgtaggta aaggtgcagc cctcctggca atactgttcg
     1261 agcccctcat tgcaggccgg gcagcggcgg caggaatcca ccatgcagcc gacgccggca
     1321 tagtcgtccg tcttgacttt ggtgacctgc gcgccgacgc gggtgacgcg gccgacgatc
     1381 tcatggccgg gcaccatcgg gtacatcgag ccggcccaat cgttgcggac ctgatggaga
     1441 tcggaatggc agatgccgca atagaggatt tcaatctgca catcgtgcgg gccgggttcg
     1501 cgccgctcga aggtgaaggg cgtcagggat gaagtagcgt tttgggcggc atagccgcgg
     1561 gtcgtgatca tattcgtgtc ctgtttgtta gattatctgc ttcgtgcgag agcagcgtag
     1621 catcatcaca accgccgggc aaggcgtttg cgcctggagc ggcgcggagg ggtgcatcct
     1681 gacactacca ccggaacgcc ggattcatcc ggcagcccca gtgcggctcg ctggcctgcc
     1741 ggatgaatcc ggcgttccga ttggcatcgc cgcccgcctt ggtggtggtg tcagtttgac
     1801 gcccgcactc ggacgttatc ctcaactgcg gatacctgga caaactcgcc ggtttcaagg
     1861 agattgtgca tgagaaaccc atagcacaaa ggaactcccg cttatgaagc ccgatgccgt
     1921 aaaacaactc gatgaagccg tcgccgtcat gctggccgat ctcaactccg ccaacatctt
     1981 gcgctcggac tggaggtgat cggcacaacg caatacctgg aggcggccaa gttgatttcc
     2041 ggcactgaat acgagtgcgg cttgtgggaa ttggaggggc gcacgatgga atggatcgcc
     2101 gagcgatcgg accctgacaa tcttgcccag cgtcaacagt ccatttccgc cttcttcgac
     2161 gggcttaagg gtgacacgta tcgggccgag caacgtccgg cggtgtattt tgtcctttgc
     2221 ctcatcgtgg cggtcgtttc ctactccatg gtccggaagg cgttccagaa gtagctcgtt
     2281 ccatccggcc ttggattttt cccccttgca tccgcccctg tggtcacggc actgtggcgg
     2341 gaaatcagtc cagacatgat atgaacttgg caatctcaag acgcctattc ctttcccggg
     2401 ccgccaccgc ctcggcggtc gccgcgttct cgtcacccct cacttcgtcc tgcgcggcag
     2461 cccagcgctc cggcgagccc gagtttcacg gcgttgattt ccacgtccac ctcgacaaca
     2521 gcaccctcga caaggcgctg gaactctccc gtgaacgggg tttcaagttt ggcatcgtcg
     2581 agcacgcggg caccaaagag aaccagtatc ccacactctt gagcaacgac gaggatatgc
     2641 ggcgctacct cgccatgctc gaaggccagc ccgtcttcaa gggcatccag gccgagtgga
     2701 cggattggat gagctgcttt tcccgccaga cgctggcgca attggattac gtgctaaccg
     2761 acgc
//
LOCUS       DATCGS010000190         6000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_67266, whole genome shotgun sequence.
ACCESSION   DATCGS010000190 DATCGS010000000
VERSION     DATCGS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67266"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VIL39_03830"
     CDS             <1..643
                     /locus_tag="VIL39_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5480585.1"
                     /translation="PCTLEFLDRTTIHCVEDFAKVGLPLDCEALLLMETDGHPGPVAD
                     EAARMEEIARKNGAVEVRVAKDDAEATKLATARRSAFSALARVSPTTILEDATVPRSE
                     LAKMIRFVASVAKKHQLRIGTFGHMGDGNLHPTFLTDERNKDEMHRVEEAFKEIFTEA
                     IRLGGTITGEHGIGVSKKSFLPKFAGDASMRVMRELRRVLDPSGVLNPGKIFD"
     gene            778..2079
                     /locus_tag="VIL39_03835"
     CDS             778..2079
                     /locus_tag="VIL39_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5480586.1"
                     /translation="MNTPFSHLKGLDYSVLQQCMHCGLCLPTCPTYDATKVERHGPRG
                     RISLMRAIADGRLGATRTFADEMYFCLGCLACMSACPAGVNYAELFEHARAEAEASGV
                     LKSAKRNMIRAFAIRWLFMDLSHLRLVGRVLRIYQQLGLQALVRGSGVLKLLPKRLQE
                     LEGITPDIQPKFSAELIAPVTPAVGPKKYRVAMLIGCAQDLIFSNINLDTVEVLARNG
                     CEVFTPPKQLCCGSLHAHNGEWKLAQQLARKQLEQFPPDQFDAIITNAGGCGSHLKHY
                     HKLLADDPAYRDRAVLWDKKVKDIHEWLVEIGVQRPTKPGPVQVVTYHESCHLAHGQK
                     ITAQPRQLLGLIPNLTLVELPESLWCCGSAGIYNIIQPEMAGQLLDRKLKHIKSTGAS
                     IVANGNPGCLLQLVNGAKQEGLNLRVVHPITLLAEAYRRSG"
     gene            complement(2168..2923)
                     /locus_tag="VIL39_03840"
     CDS             complement(2168..2923)
                     /locus_tag="VIL39_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5480587.1"
                     /translation="MKVTQPTRKASRLDFPALLGRWLLGGLLLYLGLSKVLHPAEFVK
                     LVREQLMIIDPFLSGLVVTTAPWLEMLFGLLLLTGIARNATAVLARWWLAGVFIFMGL
                     HKALPHPEAFLKLVRQYDIVTNPFLLNSIGAALPWFEVFCGLLLLAGVAVRGAALNIV
                     VMLVPFTLMVLKRALVIAAASGIPFCAVKFDCGCGAGEVYICHKLVENTVLLLLSAWL
                     LTGRGRPLCLRFSLFREKAPASDPPAPAKPPSS"
     gene            complement(2920..3573)
                     /locus_tag="VIL39_03845"
     CDS             complement(2920..3573)
                     /locus_tag="VIL39_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5480588.1"
                     /translation="MKKVLQEGALVAVIGAALAFAANALSPHRLELARDYFPATKPAS
                     PPTVAQTNLTVAAPAATNSPVEVLAASLRAKGLQLAESNQVVQFFRDPRREQDGVIFI
                     DARDEDHYRAGHIPGAYHFDRFQPQNSLTNVVQVCLTAQQMVFYCNGGECDDSEHAAI
                     MLRDSLGIPKEKVFVYGGGFTEWTNNGLPYELGQRNSGNLTNAIKAAASANVGGAKQ"
     gene            complement(3574..4737)
                     /locus_tag="VIL39_03850"
     CDS             complement(3574..4737)
                     /locus_tag="VIL39_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEY5480589.1"
                     /translation="MKNLAWAVGLTMLSTAVVGAQQTSAPPATVTTPTNAVGPKIQFA
                     TPVYEFGKIRAGEPIRYTFVFTNTGDQVLILNSVQPQCGCTTAGEWTKQVEPGGTGTI
                     PIQVNTLGYNGLVIKQVTVTCNVASQPTTFLQLKGTLYKPIDFNPPLAVLNLPPDGET
                     ASVVVTITNNTDEPLMLAYPESNNRMFSAQLVTNTPGRGFQLTVAVVPPLSTGSVQGQ
                     INLRTGWTNPVVLTVPVVANVPPAVMAIPSYITLAPGPLATAVTNSVTIQNNSTNALQ
                     LSEPVVNVPGVVAQINEIRPGRAFTATLAFPEGFTVPPGQQVELTVKSTNPKYPVIKV
                     PIMQMPRPAVYHPPATLAAPLAPVRPLTPPRPNPQAAASRPPPPPPPPLPVSR"
     gene            complement(4822..5208)
                     /locus_tag="VIL39_03855"
     CDS             complement(4822..5208)
                     /locus_tag="VIL39_03855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY5480590.1"
                     /translation="MDTALRQRVRDWAGHRCEYCRLRQEDQPVCRFHIEHIIPRQHGG
                     GDEEENLALACFHCNLHKGTNLTGVDPDSGEGDDVGCRVWQGAVRACSRAAGNIGLRH
                     VSGRHQNRCGLRITAVPLINPPSSPG"
     gene            complement(5208..5663)
                     /locus_tag="VIL39_03860"
     CDS             complement(5208..5663)
                     /locus_tag="VIL39_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480591.1"
                     /translation="MLLFLLDGLLLLRVADRQFLPLLFLRFERYLLAFKLQNQRAIFR
                     PEWQPLCFGDGDISGIRPSSMRAAIVVCFKPGRFNRIPFLRARHSSVNRRELLSTGTT
                     LAAGREFLVMMVSLPVPASRMIEAWVSACDLVAILQSKARAWLNHAHAG"
BASE COUNT         1206 a         1934 c         1774 g         1086 t
ORIGIN      
        1 cccttgcacg ctggagtttc tggaccgcac caccattcat tgcgttgaag acttcgccaa
       61 ggtcggcctg ccgctcgatt gcgaagccct cctgctcatg gaaaccgacg gccatcccgg
      121 ccccgtcgcc gatgaagccg ccaggatgga agagatcgcc cgcaagaacg gcgcagtcga
      181 ggtccgggtg gccaaggacg acgccgaggc taccaagctg gccaccgccc ggcgcagtgc
      241 tttttccgcc ctggcccgcg tctccccgac cacgattctt gaggacgcca ccgtgccacg
      301 cagcgagctg gcgaagatga tccgcttcgt ggcctccgtc gcgaagaaac accagttgcg
      361 catcggcact ttcggccaca tgggcgatgg caacctgcat cccaccttcc tcaccgacga
      421 gcgcaacaaa gatgaaatgc atcgcgtcga ggaggcgttc aaagagatct tcactgaagc
      481 catacgcctc ggcggcacca tcaccggcga gcatggcatc ggggtgtcca agaagtcgtt
      541 cctgcccaag ttcgccgggg acgcctcgat gcgcgtgatg cgcgagctcc gtcgcgtgct
      601 tgacccgagc ggcgtgctca atccggggaa gatatttgac taacgcggca agaagttaaa
      661 tgagttacaa gagttacatg gttacaaatg ccggtggcgg ggtcgctcat gtaacccttg
      721 taacgattta accttgtaac cccttgcatt gactgctgtg cctgcctgcg acattcgatg
      781 aatactccct tttcccacct caaaggcctc gactactcgg tcttgcaaca gtgcatgcac
      841 tgtggcctgt gcctgcccac ctgcccaact tacgacgcga cgaaggtcga gcgtcacggc
      901 ccgcgcggcc gcatctcgct catgcgcgcc attgccgacg gccgcctggg cgccaccagg
      961 acgttcgccg acgagatgta tttttgcctc ggctgcctcg cctgcatgtc agcctgtcct
     1021 gccggcgtca attatgccga actgttcgag cacgcccgcg ccgaggctga agccagcgga
     1081 gtgctcaaat cggccaagcg gaacatgatc cgcgcctttg ccatccgctg gctctttatg
     1141 gacctgagcc acctgcggct ggtgggccgc gtcttgcgga tctaccagca gctcgggctg
     1201 caagccctcg tgcgcggcag cggcgttctc aaactgctgc ccaaacgcct ccaggagctc
     1261 gaaggcatca cgcccgacat ccagcccaag ttctccgccg agctgatcgc ccccgtcacg
     1321 cctgctgtcg gcccgaagaa gtatcgggtc gcgatgctca tcggctgcgc ccaggacctg
     1381 atcttcagca acatcaacct cgatacggtc gaagtcctcg cccgcaacgg ctgcgaagtc
     1441 ttcacgccac cgaagcagct ctgctgcggt tccctgcacg cccacaacgg cgaatggaaa
     1501 ttggcccagc agcttgcgcg caagcagctc gagcagttcc cgcccgacca gttcgacgcc
     1561 atcatcacca acgccggcgg ctgcggctcg cacctgaagc actaccacaa gctcctcgcc
     1621 gatgacccgg cctatcgcga ccgcgccgtg ctctgggaca agaaggtcaa ggacatccac
     1681 gagtggctcg tcgaaatcgg cgttcagcgg cccaccaagc ccggtccggt gcaggtggtg
     1741 acctaccacg aatcctgcca cctcgcgcac ggccaaaaga tcaccgccca gccgcgccaa
     1801 ctactgggcc tgatcccgaa cctgacgcta gtggagttac cggagagcct atggtgctgc
     1861 ggcagcgcgg gcatctacaa catcatccag ccggaaatgg ccggccaact cctcgaccgc
     1921 aaactcaagc acatcaagtc aaccggcgcc agcatcgtgg ccaacggcaa tccgggctgc
     1981 ctgcttcaac tcgtcaacgg cgcgaagcag gagggcctga acctgcgcgt cgtccacccg
     2041 ataaccctcc tcgccgaagc ctatcgccgg tccggctaag ccgtgaggcg gattcataag
     2101 ctattaccct ctgccggaac tggcgcgagc tgcgatgatc cttcgccgag cagccttcca
     2161 attcgcgcta tgagcttggg ggcttcgcag gcgcaggcgg gtcggaggct ggagctttct
     2221 cccggaacag gctgaagcgg aggcacagtg ggcggccacg cccggtcagc agccaggccg
     2281 agagcagcaa caaaaccgtg ttctcgacca gcttatggca gatatagact tctcccgccc
     2341 cgcatccgca gtcaaatttc accgcgcaga agggaatgcc tgaagctgcg gcaataacca
     2401 gcgcccgctt gagcaccatg agggtgaacg gcaccagcat caccacgatg ttaagagcgg
     2461 cccctctgac cgccacaccg gccagcagca gcagcccgca gaacacctcg aaccacggca
     2521 acgcggcgcc gatcgagttc aacagaaacg ggttggtcac gatgtcgtat tgccgcacga
     2581 gcttgaggaa cgcctccggg tgcggcaggg ctttatgcaa ccccatgaaa atgaagacgc
     2641 cggccagcca ccacctggcc agcaccgcgg tggcattccg ggcgatgccg gtcagcagca
     2701 gcagtccgaa aagcatttcc agccagggcg cagtggtaac caccaatcct gacaagaagg
     2761 gatcaatgat catcaactgc tcccgcacga gcttcacaaa ctcggccggg tgaagcacct
     2821 tgctcaagcc caggtaaagc agcagaccgc ccaacaacca ccttcccagc agcgccggga
     2881 agtcaaggcg gctcgccttc cgggtgggtt gcgtcacttt cactgttttg cccccccgac
     2941 attggccgat gcggctgcct taatcgcatt ggttaaatta ccgctattgc gctggccgag
     3001 ctcgtacggc agtccgttgt tggtccactc cgtgaaaccg ccgccataca cgaacacctt
     3061 ttctttcggt atgccaaggc tgtcgcgtag catgatcgcc gcgtgttcgc tatcatcgca
     3121 ctccccgcca ttgcagtaga acaccatctg ctgcgccgtg aggcaaacct ggaccacgtt
     3181 ggtaagggag ttctgcggtt ggaaccggtc gaagtggtaa gcgccgggaa tgtggcccgc
     3241 ccgataatgg tcctcatcgc gagcgtcaat aaagatgacc ccatcctgct cgcggcgggg
     3301 gtcgcggaag aactgcacga cctggttgct ctcagccagt tgcaggccct ttgcccggag
     3361 gctcgccgcc agaacctcga cgggcgaatt ggtggccgcg ggtgcggcaa ccgtcaggtt
     3421 ggtctgcgcc accgtggggg gtgaagccgg cttggtcgcc gggaaatagt cccgggccag
     3481 ctcaaggcgg tgcggtgaga gtgcattggc agcaaaagcc agggccgcac cgatcaccgc
     3541 caccagcgca ccttcctgca agaccttttt cacctatcgg ctgacgggca ggggcggtgg
     3601 cggcggagga ggaggccggg aggccgctgc ctgcggattc ggccttggcg gtgtcagcgg
     3661 tctcaccggc gccagcggcg ccgccagcgt cgccggcgga tgataaacgg ccggccgcgg
     3721 catttgcatg atcggcacct taatgaccgg gtacttcggg ttggttgatt tgacggtcag
     3781 ctcgacttgc tgacccggtg gaaccgtgaa tccctccggg aaggccagcg tggcagtgaa
     3841 ggctctgcca ggccgtatct cgttgatctg cgctacaacg ccgggcacat tcacgactgg
     3901 ttcggacaat tgcagggcgt tcgtgctgtt gttctggatc gtgacggagt ttgtcaccgc
     3961 ggtcgccagc ggtcccggag ccagggtgat gtaagaaggg atcgccatga ccgccggcgg
     4021 cacgttggca acgacgggaa ccgtgagcac caccgggttg gtccaaccgg tccgcagatt
     4081 gatctggccc tgaacacttc ccgtggacag cggcggtact accgcaaccg tcaactggaa
     4141 ccctctgcca ggcgtattgg tgaccagttg ggcggagaac atacgattgt tgctctccgg
     4201 gtaagccagc atgagcggct cgtcggtgtt gttcgtgatg gtcacaacca ccgaggccgt
     4261 ctccccgtcg ggcggaaggt taaggactgc caggggggga ttgaaatcaa tcggcttgta
     4321 aagcgtgcct ttgagctgaa ggaacgtggt gggctggctc gcgacattgc aggttacggt
     4381 tacctgtttg atgaccagcc cgttgtagcc aagggtgttg acctggatcg ggatggtgcc
     4441 ggtgccgccc ggttccacct gtttcgtcca ctcgccggct gtggtgcagc cacactgcgg
     4501 ctgcacactg ttgagaatca gcacctggtc ccccgtgttg gtaaacacga aagtgtacct
     4561 gatcggctct ccggccctaa tcttgccgaa ctcatagacg ggcgtcgcaa actgaatctt
     4621 cggccccacg gcattcgttg gggtcgtcac ggttgctggc ggagccgaag tctgctgcgc
     4681 cccgacgacg gcggtgctca acatcgtgag cccaacggcc cacgccagat tcttcatcgc
     4741 aatactcata aatgacatct aaccatttat tattacagat tatgccgctt ttgcaaagcc
     4801 cattctctta cagatcgaat cctaaccagg gctgctcggc gggtttataa gtggaacggc
     4861 ggtaatcctc aaaccgcagc ggttttgatg tctgccactg acatggcgta aacctatgtt
     4921 tcccgcggct cggctgcaag cccgcaccgc accttgccaa acacggcacc caacatcatc
     4981 accctcgccc gagtcggggt caaccccggt gaggttcgtg cccttgtgca ggttgcagtg
     5041 gaaacaggcc aaagccaggt tctcctcctc gtctccaccc ccatgctgcc gggggatgat
     5101 gtgctcgata tgaaaccggc agaccggttg gtcctcctgg cgcaaacggc aatactcgca
     5161 ccggtggccg gcccaatccc gcacccgctg gcgcagcgcg gtgtccatca cccggcgtgc
     5221 gcgtgattca gccaagcccg ggctttcgac tgcaatatgg cgacgagatc acacgccgac
     5281 acccaggctt caatcatccg gctggctggg acgggtaagg aaaccatcat cactaagaat
     5341 tctcgcccgg ccgccaaggt ggtcccggtg gaaaggagtt ccctccggtt gacggaagag
     5401 tggcgggcgc gcaggaaagg aatccggttg aaccgccccg gcttgaaaca caccacgatt
     5461 gccgccctca ttgaggaggg gcgaatgccg gatatgtcgc cgtcgccaaa gcaaaggggt
     5521 tgccactctg gacgaaagat ggcccgctga ttttgaagct taaacgcgag aagatagcgt
     5581 tcaaacctta ggaacaacaa cggcaggaac tgccggtcag cgacccgcaa caacaacaac
     5641 ccgtccaaca ggaacaacaa cattgggttc cgggttgtcc tggccccagc tcaaccggcg
     5701 cgccggaggg cgcattggct gacccggccg ccatcccgtc ctgcgcaacg gactgggcgc
     5761 aggcaaacag agacagcgaa ccgcccggtg cgagtcggga agcggagctt cccaaaagct
     5821 ccgggcggtc tctcgggctg tgagatgccg cccggtccgc cagaccatct ctgaatggca
     5881 ggttgagcag caacttggtc gtgcaataca gcacgaattt ggcgggaagc aactggatga
     5941 atctgctttc cctgtgcaat ttgccggcca gcccgtatcc gttccttgac ccggctggtg
//
LOCUS       DATCGS010000191         4813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_611802, whole genome shotgun sequence.
ACCESSION   DATCGS010000191 DATCGS010000000
VERSION     DATCGS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611802"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VIL39_03865"
     CDS             <1..1182
                     /locus_tag="VIL39_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5480592.1"
                     /translation="NNYKNVVNEWKISTTNANVVDPASRREVISFGKNAGNHNGGTIT
                     FGPDGYAYLALGDGGNANDVGPSHIVPGGNAQNLSTPLGKMLRFDPLNPALTPSSPDP
                     ISTNGQYRIPTTNPFQGPGQASEIYAYGLRNPYRFCFDRANGDLIEADVGQNNIEEIN
                     RIVRGGNYGWAVKEGDFLFNQTNGPSGPAGTIGAPPGNRSPGVPAGLIDPISGTMGTL
                     EYDHNNGISITGGFVYRGTAMPELYGKYIFGDLALVASPVRVNGRLFYADLQTGQMNV
                     FTLPQFGSEILPSGLTVHGFGQDANGELYALATNTSANGTGGVVYKLASVRLSFQVSG
                     NLLDISWPIAGGRLQTQTNGPGVGIGTNWVTVPGSTTTNHVVVPIGPANGGVFYRLAV
                     P"
     gene            1205..1606
                     /locus_tag="VIL39_03870"
     CDS             1205..1606
                     /locus_tag="VIL39_03870"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY5480593.1"
                     /translation="MSSAPHDLVLFLGRFHPVLVHLPIGGLVLLGVLELLAKFPRFKG
                     AAQNNRLILGLVAAASVIAALLGWMLSRSGGYDPQLLLWHKWTGLAVAATCALAWLLN
                     WQDRPRAYRIFLLVTLAALVVASKAARTVRR"
     gene            complement(1830..2831)
                     /locus_tag="VIL39_03875"
     CDS             complement(1830..2831)
                     /locus_tag="VIL39_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480594.1"
                     /translation="MNTNNRLSLNVRRHRNPKPAFVSRFTVLAGLALAAALSPMVLAS
                     ENVPHQPFAQWADVPERGQFVFGLVYEESEAYHIWASGKQRGINVNPPDGESYGIDIN
                     QGYLALQYGITEKWAADLNIGVTTVGWRSFSPTHRIESTTGLMDWSFGVRYQIFREAL
                     ETNSPWLPTLTFRAGAVLPGSYDESFIFAPGLRSAAIEPELLLRKHFGWPGLGVYGDA
                     LYRWNRTTGNDQYIAAIGLFQQIKGWELDVGYRRMQTISGTDISFADPNDLSTIVYPR
                     DPREINDAIEAGFSYTTSKRHWRYGFYSRTVLDGNNSDSKFWVGGSIDIPIGGKHEH"
     gene            2984..4333
                     /locus_tag="VIL39_03880"
     CDS             2984..4333
                     /locus_tag="VIL39_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5480595.1"
                     /translation="MNSTNLTDSSRTTRREFLKTSGTALAGAALASAIARPGYAAENN
                     TIKIALVGCGGRGTGAAAQALSTQGPTKLWAVADVFEQHVQSSLANLKANHEKQLDVP
                     PERQFIGLDGFKKAIDSLDKGDVVLLTTPPAFRPIHFEYAVQKGVNVFMEKSFAVDAP
                     GIRRVLKTGEEAAKKNLKVATGLMSRHYKPLEETIQQIHDGLIGELITCWAYRMHGPV
                     GIGAKQPGMKELAYQIANYSSFTWLNGSFIEDWLIHNIDVCCWAKNAWPVSAQGMGGR
                     QVRQDADQLFDHYEAEYTFADGTRFVAQGRHIANCWDFFGSILQGAKGSAILGEGQTK
                     PRLFRGYQQTAENLIWSYKGPACDHYQVEHDLLFDAIRNDKPYNEAARSAKSCFTAIM
                     GRMACESGKLITWDEALASNLELAPGLGNYTWDSNPPAMPDANGKYPIAMPGQTPGV"
     gene            4424..>4813
                     /locus_tag="VIL39_03885"
     CDS             4424..>4813
                     /locus_tag="VIL39_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEY5480596.1"
                     /translation="MQKLVGILGTNASGKSELGIRLANHFGGEVVSADSRQVYRGLDL
                     GTGKITPAQAGTVKHHLIDAADVSEYFSLAQYQRAAYRAIDFIAGAGKLPLLVGGTGL
                     YVSAIVEGYELVDVPPNDPLRAELECLP"
BASE COUNT          976 a         1505 c         1443 g          889 t
ORIGIN      
        1 aacaactaca agaacgtcgt caacgaatgg aagatctcga ccacaaacgc caatgtcgtt
       61 gatcccgctt cgcgccggga ggtcatctcg ttcggcaaaa acgccggcaa tcacaacggc
      121 ggcaccatca ccttcggacc ggacggctac gcgtatctcg ctttgggtga tggcggcaac
      181 gccaacgatg tcgggcccag ccacatcgtg ccgggcggca acgcccagaa cctgagcacc
      241 ccactcggca aaatgttgcg gtttgaccca ctcaatccgg ccctgacgcc cagcagcccc
      301 gacccgatca gcaccaacgg acaataccgc atcccgacga ccaacccgtt tcaggggccc
      361 ggccaggcgt ccgagatcta cgcctacgga ttgcgcaatc cttatcgctt ttgcttcgac
      421 cgcgctaatg gcgatctgat tgaggccgac gtcggccaga acaacatcga ggagattaat
      481 cgcatcgttc gtggtggcaa ctacggctgg gcggtcaaag aaggggactt ccttttcaac
      541 caaacgaacg gtccgtccgg gcctgccggc accatcgggg cacccccggg aaatcgcagc
      601 ccgggagtcc ccgcggggct gatcgacccg atttccggca cgatgggtac actcgaatac
      661 gaccacaata acggcatttc catcaccggc ggtttcgtct atcgcggcac cgcgatgccc
      721 gagctatatg ggaagtatat ctttggcgac ctggccctgg tagcaagccc cgtgcgggtg
      781 aacggccgct tgttctatgc cgatcttcaa accggccaga tgaacgtgtt tacactgccc
      841 cagtttggct cggagattct gccgagcggc ctcaccgtgc atggcttcgg acaggacgcc
      901 aacggcgagc tctacgcgct ggcgaccaac acctcggcca atgggaccgg cggggtcgtt
      961 tacaagctcg cttctgtgcg cctgtcgttc caggtgtcag gcaacctgct ggacatttcc
     1021 tggcccatcg ccggtgggcg gctgcaaacg cagaccaacg gcccgggcgt tggcattggc
     1081 acgaactggg ttacggtgcc gggttcgacg acgaccaatc atgtcgtcgt tccaattggc
     1141 ccggccaatg gcggcgtgtt ctaccggctg gccgtgccgt gattatagag agaggctgtg
     1201 tcgcatgagc agcgctccgc atgatttggt tctcttcctt gggcgttttc acccggtgct
     1261 ggtccatttg cccatcgggg gcctggtctt gctgggcgtt ctggagctat tggcgaagtt
     1321 tccgcgcttc aagggcgcgg cccaaaacaa ccggctgatc ctcggactgg tggcggcagc
     1381 ctcggtcatc gcagccttgc tggggtggat gctctctcgc tcaggcggct atgacccgca
     1441 gttgttgctg tggcacaagt ggaccggtct tgccgtcgcc gcaacctgcg cgctggcttg
     1501 gctactgaac tggcaggacc ggccccgagc gtatcggatc ttcttgctgg tgacgctcgc
     1561 cgccctggtt gtcgcctcaa aggcggcaag aacggtccgg cgctgatcgc cggcaaagcc
     1621 tttaacagcc ctattatcca acgcctcctc ctgccgctca atgatgatga ccacatgccg
     1681 cccgacagca agccgcagcc aaccctggcg gaaattgcag cacttcagtg gtggatcgac
     1741 cacggtgcgc ccgcggacaa gacggtcggc gacctccagc ctagcgcgga gattcagcgc
     1801 atacatggcg ccgcgcaagc tggggcgaat taatgttcgt gcttcccgcc gatcgggata
     1861 tcaattgaac cgcccaccca gaacttgctg tcagagttgt tgccgtcgag caccgtccgg
     1921 gaatagaaac catagcgcca atgccgcttg gacgtggtgt agctgaagcc cgcttcaatc
     1981 gcgtcattga tctccctggg gtccctcgga taaacaatgg tgctgaggtc gttcgggtcg
     2041 gcaaaggaga tgtcggtgcc ggaaatggtt tgcatacgcc ggtagcccac gtccagttcc
     2101 cagcctttga tctgctggaa cagtccgatg gcggcgatgt attggtcgtt cccggtggtg
     2161 cgattccagc gatacaaggc atcgccgtaa acccccagtc ctggccagcc aaagtgcttg
     2221 cgcaggagca gctccggctc aatcgctgcc gagcgcaggc cgggagcgaa gatgaagctc
     2281 tcgtcatacg agccgggcag caccgcgccc gctcgaaaag tcagcgtcgg cagccagggc
     2341 gaattggttt cgagggcttc cctgaatatc tggtagcgga cgccgaaaga ccaatccatc
     2401 agcccggtcg tagattcgat tcggtgggtg ggagagaagg agcgccaccc gacggtcgtg
     2461 acgccgatgt tcagatcggc tgcccacttc tcggtgatgc catattgcag ggcgagatag
     2521 ccttggttga tgtctatgcc gtaactctcc ccgtcggggg ggttcacgtt gatgccacgc
     2581 tgcttgccgc tggcccagat gtggtaggct tcggactctt cgtacaccag cccgaacacg
     2641 aactgaccgc gctcgggcac gtcggcccac tgcgcaaaag gctggtgcgg gacgttctcg
     2701 gaggccagca ccatcggaga cagtgccgcg gccagcgcga ggccggccag gacagtgaat
     2761 cggctgacga aggccggctt cgggtttcga tgccgcctga cgttcaagct caacctgttg
     2821 ttagtgttca caagtaaagt gtttactata tcaaatcact tgtcagatgc aaccctggaa
     2881 atgggccagg aaacggctct tgccaagcgc ggggcggaag ggaatcatcc gtccgacggc
     2941 catgaacagt ttgaatctcg caaacgcaaa catcacaagc tccatgaact ccacaaacct
     3001 cacagactca agtcgcacca cgcgtcgtga attcctcaag acctccggca cggcattggc
     3061 cggcgcggcg ctggccagcg ccatcgcccg ccctggttat gccgcggaga acaacaccat
     3121 taagattgcc ctcgtcggct gcggcggacg cggaacgggc gccgcggccc aggccctgtc
     3181 cacccagggg cccaccaaac tctgggccgt ggcggatgtc ttcgagcagc acgtccaaag
     3241 cagcctcgcg aatctgaaag cgaaccatga gaagcaactg gacgtgcccc cggagcggca
     3301 gttcatcggc ctggacggat tcaagaaggc gattgattcc ctggacaaag gcgatgtagt
     3361 gctgctgacc acgccgccgg cgtttcggcc aatccacttt gagtatgcgg tgcagaaggg
     3421 cgtgaacgtg ttcatggaga agtcgttcgc cgtggatgcg ccgggcatcc gccgcgtgtt
     3481 gaagaccggg gaggaagccg cgaagaagaa cctcaaggtc gccaccggcc tgatgagccg
     3541 ccattacaaa cctctggaag aaaccatcca gcaaatccat gacggtttaa tcggtgaact
     3601 catcacctgc tgggcctacc ggatgcacgg gccggtcggc atcggcgcga aacagcccgg
     3661 catgaaggaa ctggcctacc agatcgcgaa ctatagctcc ttcacctggc tcaacggcag
     3721 cttcatcgag gactggttga ttcacaatat cgacgtctgc tgctgggcca agaatgcgtg
     3781 gccggtgtcg gcccagggca tgggcgggcg ccaggtccgc caggatgccg accagttgtt
     3841 cgaccactac gaagcggaat acaccttcgc cgacggcacg cgttttgttg cgcagggccg
     3901 gcacattgcc aactgctggg actttttcgg cagcatcctt cagggcgcca agggcagtgc
     3961 gattctcggt gaaggccaga ccaagccgcg cctcttcaga ggctaccagc agaccgccga
     4021 gaatctcatc tggagctaca aagggccggc ctgcgaccat taccaggtcg aacacgacct
     4081 gctgttcgac gctatccgca acgacaagcc ttacaacgag gccgcccgct ccgccaagtc
     4141 ctgcttcacc gccatcatgg gccgcatggc ttgcgagtcc ggcaaactga tcacctggga
     4201 tgaggcgctc gcttccaacc tcgagctggc gcccgggctg ggcaactata cctgggattc
     4261 caatcccccg gctatgccgg acgcgaatgg caaatatccc atcgccatgc cggggcagac
     4321 gccgggcgtc taaacgggcg gggattgcgg gtcaagcgct gagtaacggt aggcgtagcc
     4381 ggcgggacag gtgcgcgccc caggaccatc gatcccactg agcatgcaaa agcttgtcgg
     4441 catactcggg acaaatgcct ccggaaagag cgagctcggc attcgcctgg cgaaccactt
     4501 cggcggtgaa gtcgtgtcgg ccgactcacg ccaggtttat cgcggcctgg acctgggcac
     4561 tggaaagata accccagccc aagccgggac ggtaaaacac cacctcatag acgccgccga
     4621 tgtctccgaa tacttttccc tggcgcagta tcagcgggcc gcttaccgcg ccattgactt
     4681 tattgctgga gccggaaaac tgcccctgct cgtgggcgga accggtctat acgtcagcgc
     4741 tatagtcgag ggatatgaac tggttgacgt ccccccgaac gacccactgc gcgcagaact
     4801 cgagtgcctg cca
//
LOCUS       DATCGS010000192         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_16091, whole genome shotgun sequence.
ACCESSION   DATCGS010000192 DATCGS010000000
VERSION     DATCGS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16091"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1480
                     /locus_tag="VIL39_03890"
     CDS             215..1480
                     /locus_tag="VIL39_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5480597.1"
                     /translation="MTLLDAGSVKPPPGFMALFNGTDLTGWRGGDTFDHRKLLAMPAE
                     QREAQIAKWTETMKAHWRAEQSELVNDGQGAYATTEKDYGDFELLVDYKTVPKADSGI
                     YLRGCPQVQIWDYTEKAKFPLGADKGSGGLWNNSAGAPGKDPLVLADKPFGEWNTVRV
                     LMVGARVTVWLNSKLVVDHALLENYYDRKTPVPPKGPIELQTHGGEIRWRNIFIREIG
                     SDEANKILASHGNEGFKSIFTGRDFTGWAGPTDNYEVADGAIQCRAKKGGTIYTTDEY
                     ADFVARLQIKLPPGGNNGLAIRYPGQDDTAYQGMCELQVLDDNYEKATGDKIDPRQAH
                     GSAYGMVAAARGYQHPIGEWNFQEVTVKGPKIKVELNGTVILDCDLSTVTEFMGGHAH
                     PGKTRTKGSFGFAGHNDPVAFRNIQIKPL"
     gene            1492..2028
                     /locus_tag="VIL39_03895"
     CDS             1492..2028
                     /locus_tag="VIL39_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480598.1"
                     /translation="MQSKHEALWPTDLFTQGIGWVIIARFKSGGRRVEVGIFLIDVLC
                     LGAKLAVYEDCDASDYRRRIRDHYQSSFPMVAAEPACARKLLEQAVQYAKRLGFAPHP
                     DYKKAARVFGGLRTEQCAQQFTFGREGKPFYRRGPKETEEQARRIVEHLDCRCGSGNY
                     HYEVLLGEAEAITRFLED"
     gene            complement(2158..2394)
                     /locus_tag="VIL39_03900"
     CDS             complement(2158..2394)
                     /locus_tag="VIL39_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEY5480599.1"
                     /translation="MSTINELDPVALTGDLPAHGLKRGDVGTAVLVHGKGAAFEVEFV
                     GYDGRTVALVTLERDQVRPLQTGDIPHVRELAAA"
     gene            complement(2584..2784)
                     /locus_tag="VIL39_03905"
     CDS             complement(2584..2784)
                     /locus_tag="VIL39_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY5480600.1"
                     /translation="MKLKVDREADALYLRLDDSPIVESEEVSPGVVLDFNRQNQVVGI
                     EMMGLSKRAPQLNPQSLEFVTV"
     gene            complement(2781..3032)
                     /locus_tag="VIL39_03910"
     CDS             complement(2781..3032)
                     /locus_tag="VIL39_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY5480601.1"
                     /translation="MTYELTQHARDVLAERHIPAAWLERALREPELKQPDPADATLER
                     RYRKIPEHENRVLRVVVNTTVAPERVVSVYFDRTMKGKL"
BASE COUNT          645 a          986 c          969 g          593 t
ORIGIN      
        1 ggtggcgcac gggtgatttg cgctttcggt cgcgatggtg gagccagtgt ccggggtact
       61 cccgaattgc tcgggaaagc agaaaaagct ttattgcacc cggcaaatac ctgaaaagac
      121 agggatgaaa atgattcaaa ccgttcgctg gggcagcttt tgctccaccc tgatgctttt
      181 ggccgccggt tcccagctcg ctttgggcgc agacatgact ttgctcgacg ccggcagcgt
      241 gaagccaccc ccaggcttca tggccctgtt caacggcacg gatctcaccg gctggcgcgg
      301 gggcgacacc ttcgatcatc gcaagctcct cgcgatgcct gccgagcagc gcgaggcgca
      361 aatcgcgaag tggacggaaa ccatgaaggc ccactggcgc gccgaacaga gcgagttggt
      421 gaatgacggc cagggcgcct acgccaccac cgagaaggac tacggggatt tcgagttgct
      481 ggttgattat aagaccgtgc ccaaagccga ttcgggcatt tacctgcgcg gctgcccgca
      541 ggtccagata tgggattaca ccgagaaggc gaagttcccg ctcggcgccg acaaaggctc
      601 cggcggtctc tggaacaaca gcgccggcgc gccgggcaag gacccgttgg tgctggctga
      661 caaaccgttc ggcgagtgga atacggtccg cgtcctcatg gttggcgcgc gagtgaccgt
      721 ctggctgaat agcaagctcg tggtggatca cgcgctgctg gagaattact acgaccgcaa
      781 gacgcccgtg ccgcccaaag gcccgattga actccagacc catggcggcg aaatccgctg
      841 gcgcaatatc ttcatccgcg agattgggtc cgatgaggcc aataagattc tcgccagcca
      901 tggcaacgaa ggcttcaagt ccatcttcac tggccgcgat ttcaccggct gggccgggcc
      961 aacggataat tacgaggtcg ccgatggcgc catccagtgc cgggcaaaga aaggcggcac
     1021 catctacaca accgacgaat acgctgattt cgtggcccgc ctgcagatca agctgccgcc
     1081 cggaggcaac aacggcctgg cgatccgcta ccccggccag gacgacaccg cttaccaggg
     1141 catgtgcgaa ctccaggtgc tcgacgacaa ttacgaaaag gccaccggcg acaagattga
     1201 cccccgccag gcccacggtt cggcgtacgg catggtcgcc gccgcacgtg ggtatcagca
     1261 ccccatcggc gagtggaact tccaggaggt caccgtcaag ggcccgaaga tcaaggtgga
     1321 gctaaacggg acggtcatcc tggactgcga cctatccacc gtgacggagt tcatgggggg
     1381 gcacgcccat cccggcaaga cccgcaccaa gggctctttc ggcttcgccg gccacaacga
     1441 cccggtcgcc ttccgcaata tccagatcaa gccgctctga aattggcacc cttgcagtct
     1501 aagcacgaag cgctttggcc gacggatctt ttcacgcagg gcattggctg ggtcatcatc
     1561 gcccgattca agtcaggcgg aaggcgtgtc gaagttggga tcttcttgat cgacgtgttg
     1621 tgcctggggg ccaagctggc ggtttatgag gactgcgacg cgagcgacta ccgtcggcgc
     1681 atccgcgacc actaccagtc gagctttccg atggtcgccg ctgagccggc gtgcgcgcgc
     1741 aagttattgg agcaggctgt ccagtacgcc aagcgccttg gatttgcgcc acatccggac
     1801 tacaaaaagg cagcacgggt gttcggaggg ttgcgaaccg agcaatgcgc gcagcagttt
     1861 acctttggac gcgagggcaa gccgttctat cggcgcggcc ccaaggaaac cgaggagcaa
     1921 gcccgtcgca ttgtggagca tctcgactgc cgctgtgggt ccggaaacta tcattacgag
     1981 gtcctgctgg gtgaggccga ggccatcacc cgctttcttg aagattaggc aagcgtgctg
     2041 acagcgaagg cgcgaaggtt tgttggccgc agattatctt acgcggtctg gcactccaac
     2101 tcgacgacgc tgactgagcg cgccgggaag cgcctcactt gccgcagtaa cagagaatca
     2161 ggcggcagcc aactcgcgga cgtgggggat gtctcccgtc tggagcggac gcacttggtc
     2221 gcgctcaagg gtcaccagtg ccaccgtgcg gccatcgtag cccacaaact ccacctcgaa
     2281 cgccgcgccc ttcccatgca ccagaaccgc cgtgcccacg tccccgcgct tcaagccgtg
     2341 cgcgggaaga tcgcccgtca gcgccacagg atccagttca ttgatcgtgc tcataacagc
     2401 ccattgtgcg tttggcagct tcacctgcgc ggtatcctaa cgaggcgctt tgggaggggc
     2461 aactggaatc cgttgaagcg gatacctccg gttgcaggcc tggtcgtgct ggcaacacgt
     2521 ggcgcgggcg attacgccaa cctgatctcg cagcctccaa agggcacttt catgcccgcg
     2581 ccctcaaacg gtcacgaact ccagagactg cggattcaac tgcggggcgc gcttggagag
     2641 ccccatcatc tcgatgccaa ccacttggtt ctgccggttg aaatccagca cgacgcccgg
     2701 cgacacctct tcagattcga cgattggcga gtcgtccagg cgcaggtaaa gagcgtccgc
     2761 ttcgcggtca accttcagtt tcatagcttg cctttcatcg tgcggtcgaa atatacactc
     2821 accacccgtt ccggggcaac cgtggtattg acgaccaccc gcagcacccg gttctcgtgt
     2881 tccggtatct tgcggtagcg gcgttccagc gtggcatccg ctgggtcggg ctgtttcaac
     2941 tccggctcgc gcagcgcgcg ctccagccat gccgcgggaa tatggcgttc cgccagcaca
     3001 tcgcgagcat gttgggtcaa ttcgtaagtc attccattat cgcgccatgc tgcggcggag
     3061 cctggcggga tgcacgccaa ttcttgaggt cacaatttgt gacttcaagt cttctaatcc
     3121 attgtgatcg agctgaaact tgtggcgcgg ctcgctggag atcatcaaat tcaccggcga
     3181 agggcgcgac ggc
//
LOCUS       DATCGS010000193         5668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_16109, whole genome shotgun sequence.
ACCESSION   DATCGS010000193 DATCGS010000000
VERSION     DATCGS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16109"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1110
                     /locus_tag="VIL39_03915"
     CDS             97..1110
                     /locus_tag="VIL39_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5480602.1"
                     /translation="MMHSLFAAERRNFRMAALLTLTGLAISLQPALAEFKVGLVLDRG
                     GKDDKSFNSSAHEGATRAKTKLGILLKYVEAPDDNAFEPMLRAFALRDFDLIIGVGFA
                     QKEAIQKVAAQFPNRHFAIVDSQVQAPNVRSLMFQEHEGAYVIGAIAAMTSKTGKIGF
                     VGGMDIPLIRRFVMGYEAGAKKINPQITVIANYVGVTSEAWNNPPKGKELAMSQYEGG
                     VDIIFAAAGASGLGVFDAAEDKKKFAIGVDSNQDWTKPGLILTSMLKRVDEAIFSTIA
                     EAKAGTFTGGVQWFGLANKGVDYSFDQYNAKILTEPVRKRADELKAEIIAGKIVVPDY
                     YKK"
     gene            complement(1118..1684)
                     /gene="def"
                     /locus_tag="VIL39_03920"
     CDS             complement(1118..1684)
                     /gene="def"
                     /locus_tag="VIL39_03920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5480603.1"
                     /translation="MPLTVVKYGTPVLRQKGARIESITPAIKKLAADMLETTYAYRGI
                     GLAAQQVGVALQLMVIDVRGVTDRPSSLELKGQPADLNKFMPLVLLNPEVKPAGQPVA
                     SPEGCLSFPEIYADVTRPEVVDVKALSLEGKPLEFRCGGLLARAIQHETDHLNGILFI
                     DRMDKETKADVRDELDLLMAETKAMLKR"
     gene            complement(1687..2790)
                     /locus_tag="VIL39_03925"
     CDS             complement(1687..2790)
                     /locus_tag="VIL39_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5480604.1"
                     /translation="MREQTATQLLAAVSRTSQMTAFVGLDGFVDDILHVVDKRESADK
                     YLRLPTIAQLAERLAGAAGRSTNLELVSQLNKLGGNGPIMANALASFGLKVTYLGILG
                     YPNLHPVFADFAKRAEVHSIAEPGYTDALEFEDGKIMLGKHESLKQMNWENIKARFGQ
                     DKFSAKFGSADLVGFVNWTMLTHMSDIWAAVLKEICPGMKGPRRKLFIDLADPEKRTK
                     GDILRALELVAAFQKHFDVVLGLNEKEGIEVGKTLGLKTSDHSPEGLTTLCQAIHERV
                     HVDTLIIHPTAYALASGPDGTALVQGPFTPKPKITTGAGDHFNSGFCLGKLLGFPTDR
                     CLLIGVATSGFYVRTGQSPAIPDLAHLLRNWPI"
     gene            complement(2877..3704)
                     /locus_tag="VIL39_03930"
     CDS             complement(2877..3704)
                     /locus_tag="VIL39_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY5480605.1"
                     /translation="MGAASAADLRERVQRVALDRVHPCPFQPRKDFSPETLRELADSI
                     KEQGIVQPLIVRERQGHLELIAGERRWRAAQLLGLKEVPVILREADDRAVLELALIEN
                     LQRENLNAIEEAQGYSQLIEQFQLKQEEVATKVGKSRAVVANALRLLKLPPSIQTSIR
                     EGRLSVGHAKVILGLAGETQQRLAFERILKEGLNVRQTEGLVARLQAREAGPVTKSGP
                     ITPLNRDTHVASLENQLRERLGTKVHLRYAHGRGALEVAFFSDAELERILLILGVTE"
     gene            4256..>5668
                     /locus_tag="VIL39_03935"
     CDS             4256..>5668
                     /locus_tag="VIL39_03935"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEY5480606.1"
                     /translation="MVTNTKRPVTISPLAGKPVPKEMLVDLTRLEREYYKRQPDVGNR
                     DQMVSFGTSGHRGSSLRGTFTEAHVLAITQAICDYRRRQGIDGPLHMGKDTHALSGPA
                     QRTALEVLAANNVQTIIQRDDGVTPTPVISRAILVYNRGRKEHLADGIVITPSHNPPE
                     DGGLKYNPPHGGPADTSVTKWVQDRANELLREDDDDVKRVPFATAIQAGTTLEEDIIL
                     PYVEDLRNVIDMEAIRAAGLKLGVDPLGGASLPYWEPINSIYGLDITVVNSKLDPTFS
                     FMTVDHDGKVRMDCSSPYAMAGLVRLKDQYRVAFANDPDSDRHGIVTPSAGLMNPNHY
                     LAVAIGYLLTHRPGWGAAAAVGKTLVSSSMIDRVVSKLGRRLAEMPVGFKYFAPGLLD
                     GSFCFGGEESAGASFLRQDGTVWTTDKDGILLNLLAAEITARTGKDPSEHFQTLVAEF
                     GKPFYTRIDAAATPEQKARLE"
BASE COUNT         1070 a         1631 c         1873 g         1094 t
ORIGIN      
        1 tgcccggctc tacggcaggc aggatgcccg ccgctacggc acagggcgtg acgccaaagg
       61 ttgaccgggc agcggtttac gcctagaaag tgggaaatga tgcacagtct ctttgcggca
      121 gagcgaagga atttccgaat ggcggcgttg ctcacgctga ccggcctcgc gatttcattg
      181 cagccggcct tggcggaatt caaagtcggg ttggtcctgg accggggcgg caaggacgac
      241 aagtcgttta actcgtcggc acacgagggg gcaacccgcg ccaagaccaa gctgggaatt
      301 ctcctgaagt atgttgaggc gccagacgac aatgccttcg agccgatgtt acgggcgttt
      361 gcgctgcgcg acttcgacct gatcatcggg gtgggttttg cccagaagga ggcgattcag
      421 aaggtcgcgg ctcagtttcc caaccggcat ttcgccatag tggactcgca ggtgcaggcg
      481 ccgaatgtgc ggtcgctcat gtttcaggag catgaagggg cctatgtcat cggcgccata
      541 gcggccatga cgagcaagac gggcaagatc gggtttgtgg gtgggatgga tattcccctg
      601 atccgccgct ttgtgatggg ctacgaggcc ggggcgaaga agatcaatcc ccagattacc
      661 gttattgcca actatgtcgg ggtcaccagc gaggcctgga acaatccgcc taaggggaaa
      721 gaactcgcca tgtcgcagta tgagggcggc gtggatataa tcttcgccgc agcgggggcc
      781 tcgggcctgg gagtgttcga cgcggcagag gacaagaaga aattcgcgat cggcgtggat
      841 tccaaccagg actggaccaa accagggctg atcctgacca gcatgttgaa gcgggtggat
      901 gaggcgatct tttccacgat tgcggaagcg aaagcgggga cattcaccgg cggcgtccaa
      961 tggttcgggc tggccaacaa gggcgtggac tactctttcg accaatacaa cgcgaagata
     1021 ctgactgaac cggtgcgtaa gcgggccgac gagctgaagg cggagatcat cgcggggaag
     1081 atcgtggtcc cggattacta taagaagtag agggggctta ccgcttcagc atcgcctttg
     1141 tctcggccat gagaaggtct agttcgtccc tgacgtctgc cttggtctcc ttgtccatcc
     1201 gatcaatgaa gaggatgccg ttgaggtggt cggtctcgtg ttggatggcg cgggcgagca
     1261 ggccaccgca gcggaattcg agcggcttgc cctccaggct tagcgccttc acgtccacca
     1321 cctcggggcg ggtgacgtca gcgtagattt cggggaagct caaacagcct tccgggctgg
     1381 ccactggctg gccggccggc ttgacttcgg gattaagcaa tacgagcggc atgaacttgt
     1441 ttaggtcggc cggttgcccc ttcaactcga gtgaggatgg acgatcggtc acgccgcgca
     1501 cgtcaatgac cattagttgt agcgcgacgc caacctgctg cgcagcgagc ccgatacctc
     1561 tgtaggcgta cgtggtttct aacatgtcgg cagcgagctt cttgatcgcg ggcgtaatgc
     1621 tctcgatgcg cgcgcccttc tggcggagca ccggggtgcc gtatttgaca acagtcaggg
     1681 gcatactcaa attggccagt ttcgcagcag gtgggccagg tccggtatgg ccggactttg
     1741 gccggtgcgc acgtaaaagc cgctggtggc gacgccgatg agcaggcagc ggtcggtcgg
     1801 aaagccgagc aacttgccca ggcagaagcc ggaattgaaa tggtcgccgg cgccggtcgt
     1861 gatcttcggt ttgggggtga agggaccctg cacgagcgcc gtgccatccg gcccggaggc
     1921 cagcgcatag gccgtcgggt gaatgataag ggtgtccacg tggacgcgct cgtgaatggc
     1981 ctggcacagg gtcgttaacc cttccggcga gtggtcgctg gtcttcaagc caagtgtctt
     2041 cccaacctcg atgccttcct tctcgttcag gccgaggacg acgtcgaaat gcttttggaa
     2101 ggcggccacc agctccaggg cgcgcaggat gtctcctttg gtgcgcttct cggggtcggc
     2161 gaggtcaatg aagagcttgc ggcgcgggcc tttcatgcca gggcaaatct ccttgagcac
     2221 ggcggcccag atgtcgctca tgtgcgtgag catggtccag ttgacgaagc cgacgagatc
     2281 ggcgctgccg aacttggcgc tgaatttgtc ctggccgaac cgggccttga tgttctccca
     2341 gttcatctgc ttaagcgact cgtgtttgcc gagcatgatc ttgccgtcct cgaactccaa
     2401 cgcgtcggtg tagcctggct cggcgatgga atggacctcg gcacgcttgg cgaagtcggc
     2461 gaacaccgga tgcaggttgg ggtagccgag gatgccgagg taggttactt tgaggccgaa
     2521 gctggcgagt gcattggcca tgatggggcc gttgccgccg agcttgttaa gctggctgac
     2581 caattcgagg ttggtgctcc tgccggcagc tccggccaag cgttcggcga gctgagcaat
     2641 cgtcggcagg cgcagatatt tgtctgcgct ctcgcgcttg tccacgacgt ggaggatgtc
     2701 atctacgaag ccgtccagac cgacaaaggc ggtcatctgg ctggtgcggc taacggcggc
     2761 taggagttgg gtggcggttt gttctcgcat ggggagatgg agtaatggag taatggagtt
     2821 gtggtggtta actgtaggct acgttgcttt cctttaggag atcatccacg actccatcac
     2881 tccgtcactc ccagtatcag gaggatgcgt tcgagttcgg cgtcgctgaa gaaggctacc
     2941 tccaaagcgc ctcggccatg ggcgtagcgc aaatgaacct tggtccccag acgttcgcgc
     3001 agttggtttt caaggcttgc gacgtgggta tcccggttca gcggggtaat gggcccggac
     3061 ttggtgacgg ggccggcctc ccgggcttgc aagcgcgcga ccaacccctc ggtctgacgg
     3121 acgttgagcc cttccttgag gatgcgctcg aacgcaagcc tctgctgggt ctcaccggcc
     3181 aatccgagaa tgaccttggc atgaccgacc gaaaggcggc cttcgcggat gctggtctga
     3241 atcgagggcg gcagcttgag caggcgcaac gcgttagcca cgacggcgcg gctcttgccg
     3301 accttggtcg ccacttcttc ctgcttgagt tggaactgct cgatgagttg cgagtagccc
     3361 tgggcctctt caatcgcgtt aaggttttcg cgctgcaagt tctcgatcag ggccagttcg
     3421 aggacggcgc ggtcgtcagc ctcccgcagg ataactggaa cttcctttag gccgagaagt
     3481 tgcgcggccc gccagcggcg ttcgccggcg ataagttcca ggtgcccctg gcgttcacgg
     3541 acaatcagcg gctgaacgat gccctgttcc ttgatcgaat cggccagttc gcggagagtc
     3601 tcaggggaaa agtccttgcg gggctggaag ggacaggggt gaacgcgatc aagcgcaacg
     3661 cgctgcacgc gctcgcgcag atcggccgct gaagcggcac ccaagattgt ggttgagttt
     3721 ggcgccggaa cgggtggttt tgcgatgggg ctgtcgccca acaacgcacc cagaccccgg
     3781 cccaaagctg gctttgccat gcatccagag tgtcgcaggg caagggagtt gtcaattcag
     3841 tccacgcgcc ggtccacgag ttgactgggc cttcctccgc tcacccttgc ggcgcgcgcc
     3901 aactggtggt gtggcgttgt tgtggtcttg gtctcaggtg ggatgccccg ggcgtcatac
     3961 tgccgatcct ccttaggtgc cacgggggcg cacacttccc cgcccgaccc caccaatcag
     4021 gggataaacc accccaaatt cgagcccagc gcgcccacca ggcgtctttg gggagcaagg
     4081 gtggggttgc agacccccgc catgcttttc aggttacaaa ttgctctcag gggcaggggc
     4141 aaacggtggc gggaacccaa acagaggatg cttcatgtat cacgcatcac gcatcacgcc
     4201 agtttgttcc tgcaatgttg aacttgcgac ggatacacgt tagaataggc caccaatggt
     4261 tacgaatacg aagagacccg tgacgatttc tcccctcgcg ggcaagccgg tgccgaagga
     4321 gatgctggtg gacctgacgc ggcttgaacg tgagtattac aagcgccagc cggacgttgg
     4381 caaccgggac cagatggtca gcttcggcac ctccgggcac cggggcagct ccctgcgggg
     4441 cacgttcacc gaggcgcatg tcctggcgat cacgcaggcg atctgcgact accggcgccg
     4501 ccagggcatt gacggcccgc tgcacatggg caaggacacc cacgcgctgt ccgggccggc
     4561 tcagcgcacc gccctcgaag tgctggcggc caacaacgtc cagaccatta tccagcgcga
     4621 cgacggggtg actcccacgc cggtcatctc ccgggccatc ctcgtctata accggggccg
     4681 caaggagcac ctggctgacg gcatcgtcat cacgccatcg cataacccgc cggaggacgg
     4741 cgggctcaag tacaatccgc ctcacggcgg cccggcggat accagcgtga cgaagtgggt
     4801 tcaggaccgg gcgaacgagc tgctgcgtga ggacgatgac gatgtgaagc gcgtgccatt
     4861 cgccacggcc atccaggcgg gaaccaccct cgaggaagac atcatcctgc cctacgtgga
     4921 ggacctgcgg aacgtcatcg acatggaagc cattcgtgcc gccgggctca agctgggagt
     4981 ggacccgttg ggcggggcct cgttgcctta ctgggagccg attaactcca tctacgggct
     5041 ggacatcacc gtggtcaact cgaagctcga tccaaccttc tcatttatga ccgtggatca
     5101 cgacggcaag gtccgcatgg attgctccag cccttacgcg atggcggggt tggtccggct
     5161 gaaggaccaa taccgcgtgg cctttgccaa cgaccccgat tccgaccggc acggcattgt
     5221 gacgccgtcg gccgggttaa tgaaccccaa tcattatctg gcggtggcga ttggctacct
     5281 gcttacgcac cggcccggct ggggcgcggc ggcggcggtg ggcaagacgc tggtcagcag
     5341 cagcatgatc gaccgggtgg taagcaagct gggccggcga ttggccgaga tgccggtggg
     5401 cttcaagtat ttcgcgccag gcttgctcga cggctcgttc tgcttcggcg gggaggagag
     5461 cgccggggcg agtttcctgc gccaggacgg cacggtttgg acgacggaca aggacggcat
     5521 cctgctgaac ctgctggcgg cggagattac cgcgcgcacc ggcaaagacc cgagcgagca
     5581 ctttcaaacc ctggtagcgg aattcgggaa gccgttttac acgcgcattg atgccgctgc
     5641 cacgcccgag cagaaggcgc gcctggag
//
LOCUS       DATCGS010000194         4540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_661976, whole genome shotgun sequence.
ACCESSION   DATCGS010000194 DATCGS010000000
VERSION     DATCGS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661976"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VIL39_03940"
     CDS             complement(<1..667)
                     /locus_tag="VIL39_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480607.1"
                     /translation="MAAAIASQSAEVLSQQKFTAGPSGEAARRFTFDSDQALTNWTIT
                     GDVTIDRTKGRGGRAGALKVGPGAKALLKLRDRDESGQVDFWIYDDGTTPENVKTNRP
                     GPRWGLVQNDGRVLAVGILYASYLGGDEGYTATACEGQSWFDQLFWLGVNRAPAGWHK
                     WTFIFDTEKGVQILHADQNGKPTRQPQFDNTKAGLRGFSAIAISGDSGAGKGQTFWVD
                     DVSV"
     gene            1016..3832
                     /locus_tag="VIL39_03945"
     CDS             1016..3832
                     /locus_tag="VIL39_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480608.1"
                     /translation="MQMLLRAKFLLGACIRPSLLCGILLLASGLANAAAPAELPPIRI
                     GPKSADATPERRAHPQPTVPDSASEIPFDETAPEPTLTGPEKERGYLLFQRPLTESIY
                     PNSRPLTYERIEALVAFATPGEFEPVTFALYPVRPLQNLKVRTSSLTSPAGEIPAGNI
                     DVRLATYWNVGYPAYTTLNTYRRVPELLERVTVHSSPAGECQWYWLTVHVPEAARPGL
                     YRGILRVWDDGFGQAIEIPLALRVLGFRLQKDPRKHYSAYFYTRNKALYKGCDEAFIR
                     RAADKDYQAMLDFGLDMLPTLYVRSEDGKHITLADAGELDRMLAAGLKGPAPVTADNV
                     IARIYRDTTPGGNRGSHWRVSPLPPPAFYERVTELFREFEVERKAKGWPEFICCPIDG
                     VDASCKEFGTKVYAAVKAAGLRTYATKDPVGADASDYAPYLDIWCSQPYSVPYDRIVS
                     QNRYEYWCYPNHNAGEIKDRRTMCKGGRMTYGFGFWRSGYTTLIPWHWCWPSEPDPFD
                     YLRGRFSGCGQRVDDDGEVIPAVYWACFREGYDDARYLYTLQQAIVDREGSKDPACLA
                     AVNQGQRLLQETWEAIRVQPKYLAEGMWPSEEFDAIRWRLAVLSARLLEYPATAKAIA
                     PSVLLAGPVRTKSAVETRSPYETAARLGRLETFDLGGGFVGWKNGTAEGNTEITEAAR
                     HVGKAGLRWTVVVDHEHDGGEGGNYPVGWPRLGRDFKSGELDMSQYDTLEFWMRIDSN
                     RDEVADDHTPVGLVISSHGRKKALYEKTLDLGGEQRVWVLLRFPVKPMMAAAEAGTEP
                     WKSISRIQLYISEHDYRHGTRLVFDVGEISLLRLTAPVLAGMDAPHHVLLPGKTLAFA
                     FDVVGVGSVTRGNYTVAAALEAGPGVVRAQARQDLADPHRVALSLSAAEPGACTLRLT
                     LLDTAGNKCSETAQPVTMHAGPLY"
     gene            3865..>4540
                     /locus_tag="VIL39_03950"
     CDS             3865..>4540
                     /locus_tag="VIL39_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480609.1"
                     /translation="MMINSTKYEGKIMRKQEKNGGTQTRRQFLAQSSVLAGASVVGAP
                     DLSRSAYAAGSDLIKIGLIGCGGRGSGSIANALSVNPGARLYAMADAFADRLDSARTQ
                     IQQKFSSQVDVNNRCFAGFDAAQKLIESGVQVALLTTTPHFRPMHIEACVEAGVHVFA
                     EKPMAIDAPGVRRVLAAGEKARQKKLSFVSGFQTRYSPAAREEVKRVHDGDIGDIVSI
                     EGVYNTG"
BASE COUNT          838 a         1460 c         1414 g          828 t
ORIGIN      
        1 tcacggagac atcatccacc caaaaggtct gtcctttccc cgcaccgctg tcgcccgaaa
       61 tggcgatggc gctgaagcct cgcaggccgg ccttggtgtt gtcgaattgc ggctggcgcg
      121 tgggcttgcc gttctggtct gcgtggagga tctggacccc cttttcagtg tcgaaaatga
      181 aggtccattt gtgccagccg gcaggggcgc ggttcacacc cagccagaat aactggtcga
      241 accaactctg gccctcgcac gctgtcgcgg tgtagccttc gtcgcccccg agatagctcg
      301 cgtagaggat tcccacggcc agcacccggc catcgttttg caccaggccc cagcgcggcc
      361 cggggcggtt ggttttcacg ttttcgggcg tggtgccgtc gtcgtaaatc cagaagtcga
      421 cctggcctga ctcgtccctg tctcggagtt taagcagggc cttggcacca ggtccgacct
      481 tgagcgcgcc agccctgccg ccgcggcctt ttgttcgatc aatggtcaca tcgcccgtga
      541 tcgtccagtt ggtcagggcc tggtcgctgt caaaggtaaa ccttctggca gcctcgccgg
      601 atggcccggc agtgaatttc tgctggctca ggacctcagc cgattggctt gcgatggctg
      661 cagccaagac aagcagactg agctttaacg cgtgtttcca catacatctt atttaccatg
      721 aatggccaag ccttcgcgac cctcgattgc tggccaccta aagttatagc tgaagtgtca
      781 acaaccttgg agaagaacag aatccgcttg aatgttgaaa ggccgccctg tcccatggga
      841 ggcggcagcc agaggtcatg tgcctctatg gtcaactaag cagttatgaa tgccttgagc
      901 cgtcggactt tccgcaaagc tagcgtcgcc gccaaacccg gcatcgagcg cctggacgca
      961 cgcaccatcc gctgggtgac cagagatccc ccggctatcc tgccttgagg cacccatgca
     1021 aatgcttttg cgcgcaaagt ttctccttgg agcctgcatc cggccctcgt tgctgtgcgg
     1081 catcctgctc ctggcgtcgg gtttggccaa cgcggcggcg cccgcagaat tgccgcccat
     1141 tcggatcggt ccgaagtcgg ccgacgccac ccccgagcgg cgcgcgcatc cgcaacccac
     1201 ggtgccagac agcgccagcg agatcccttt tgacgagacc gctcctgagc caacccttac
     1261 tgggccggaa aaggagcgcg gctatctgct gttccagcgc ccactcaccg agagcatcta
     1321 tcccaacagc cgccccctga cctatgagcg gattgaggcg ctggtcgcct tcgccacgcc
     1381 cggcgagttc gagcctgtca cgtttgccct ttatcccgtg cgtcccctgc agaacctgaa
     1441 ggtcaggacg tcgtcgctca cctcccccgc gggcgagatc cccgctggca acatcgatgt
     1501 gcgcctggcc acctattgga acgtcggata cccggcctat acaacgctta acacctaccg
     1561 ccgcgtgccg gaactactcg aacgtgtcac ggtccactcc tcgcccgccg gcgagtgcca
     1621 atggtactgg ctcaccgtcc acgttcccga ggccgcaaga cccggccttt acagggggat
     1681 cctccgcgtc tgggacgacg gcttcggcca ggccatcgag atcccgctgg ccctgcgcgt
     1741 cctcggcttt cggctgcaga aggatccgcg caagcactac tcggcctatt tctatacacg
     1801 aaacaaggcc ctgtacaaag ggtgcgacga ggcattcatc cggcgagccg cggacaagga
     1861 ttaccaggca atgctcgact ttggcctgga catgctgccg acgctctacg tgcgttctga
     1921 ggacggaaaa cacatcacgc tggcggacgc cggcgaactc gaccggatgc tggccgccgg
     1981 cttgaagggg ccggcgccgg ttaccgcgga caacgtcatt gcccgcatct accgcgacac
     2041 gacgcctggc ggaaaccgcg gcagccactg gcgggtcagt ccgctgcctc cgccggcctt
     2101 ctacgagcgt gtcaccgaac tcttccgcga gttcgaggtg gagcggaagg cgaagggttg
     2161 gccggagttc atctgttgcc cgatcgacgg ggtggatgcc tcctgcaaag aattcggcac
     2221 caaggtctat gccgcggtga aggccgccgg ccttcggacc tacgccacca aggatcccgt
     2281 cggggccgat gcctccgatt acgcccccta tcttgatatc tggtgttccc agccttattc
     2341 cgtgccctac gaccgcatcg tttctcagaa tcgctacgag tattggtgtt acccgaacca
     2401 caacgctggc gagatcaagg accgccgcac catgtgcaaa ggcggacgga tgacttacgg
     2461 cttcggcttc tggcgcagcg gttatacgac gctgatcccc tggcactggt gctggccatc
     2521 cgagccggac cccttcgact acctccgcgg ccgcttctcg ggttgcggtc aacgggtgga
     2581 cgacgacggc gaggtcatcc cggccgttta ttgggcctgc ttccgcgaag ggtatgacga
     2641 cgcgcgctac ctctataccc ttcaacaggc gatcgtggac cgcgagggat caaaggaccc
     2701 ggcctgcctt gcggcggtca accaggggca gcggctcctc caggagacgt gggaggcgat
     2761 ccgcgtccag ccgaagtacc tggccgaagg gatgtggccg tcggaggagt tcgacgccat
     2821 ccggtggcgc ctggcagtcc tgtcggcgcg gttgctggaa tatcccgcca cagcaaaggc
     2881 gattgccccg tcggtcctgc tcgccggccc tgtccgcaca aagtccgcgg tggaaacccg
     2941 gtcaccctac gagacagccg cccgcctggg gcgcctcgag accttcgacc tgggtggcgg
     3001 cttcgtcggc tggaagaacg ggaccgcgga ggggaacacg gagatcacgg aagcggcacg
     3061 gcacgtcggc aaagccggcc tgcgctggac agtcgtcgtc gatcacgagc acgacggcgg
     3121 cgagggcggc aattacccgg tcggctggcc ccgattaggc cgggatttca aatcgggtga
     3181 actggacatg agccagtacg acacgctcga attctggatg cgcatcgatt ccaaccggga
     3241 tgaggtggcc gatgaccaca cgccagtcgg gctggtcatc tcgtcgcacg ggcgaaagaa
     3301 ggcgctctac gagaagacgc tggatctggg cggcgagcag cgcgtgtggg tactcttgcg
     3361 gtttccggtg aaaccgatga tggcggcggc ggaagcgggg acagagccct ggaagtcgat
     3421 cagccgcatc caactctata tctccgagca cgactaccgg cacgggacgc ggcttgtctt
     3481 cgatgtgggt gagatatcgc ttttgcggct cacggcgccg gtgctcgccg ggatggacgc
     3541 gccccaccac gtgttgctgc caggcaagac gctcgctttc gcgttcgacg tggttggggt
     3601 cggctccgtc acccggggaa actacacagt cgccgccgca ctggaagcgg gaccaggagt
     3661 agtgcgcgcc caggcccggc aggacctggc ggacccgcac cgggtcgcgc tgtcgctgtc
     3721 ggctgctgag cctggcgcct gtacgctgcg gctaaccctg ttggataccg cgggcaacaa
     3781 gtgttcagag acggcgcagc cagtcacgat gcacgccggg cccctctact gagaagcgag
     3841 attggaacag cggccaatca actaatgatg attaactcaa ccaaatacga aggaaagatt
     3901 atgcgcaagc aggaaaaaaa cggcggcacc caaacccggc gtcagtttct ggcgcaatcg
     3961 agtgtgctag caggtgcctc ggtcgtcgga gccccggatc tcagccgaag cgcctatgcg
     4021 gcgggcagtg acctcatcaa aatcggcctg atcggctgcg gcggacgcgg gtccggctct
     4081 atcgctaacg ccctttcggt gaatccgggc gctcgacttt acgccatggc cgacgccttt
     4141 gccgatcgac tggacagcgc ccgcacccag atccagcaaa agttcagcag ccaggttgac
     4201 gtgaacaacc ggtgctttgc cggcttcgac gcggcgcaga agctgatcga gagtggtgtg
     4261 caggtggcgc tattgacgac gacgccgcac ttccggccga tgcatatcga ggcctgcgtc
     4321 gaggccggtg tccatgtgtt tgcggaaaag ccgatggcga tcgatgcccc cggggtgcgc
     4381 cgcgtgctgg cggccggaga aaaagcgcgc cagaagaagc tcagttttgt gagcggcttc
     4441 caaacccgct attcgccggc tgcccgcgag gaggtcaagc gtgtgcacga cggggatatc
     4501 ggcgacatcg tgtccatcga gggcgtctat aataccggcc
//
LOCUS       DATCGS010000195         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_513202, whole genome shotgun sequence.
ACCESSION   DATCGS010000195 DATCGS010000000
VERSION     DATCGS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513202"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VIL39_03955"
     CDS             complement(<1..380)
                     /locus_tag="VIL39_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5480610.1"
                     /translation="MPPSRQPANASPPLPGSETPKPNSSGGKMAFIVVLCLLVVAGAV
                     WLARSRAAGADKKGPGAGRMELPPVPVVAGTVAEKDVPIYLGGSGTVQAFNTVTIHAR
                     VDGQVQKIAFTEGQDVRTGDLLAQ"
     gene            complement(430..834)
                     /locus_tag="VIL39_03960"
     CDS             complement(430..834)
                     /locus_tag="VIL39_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480611.1"
                     /translation="MNSLGKRKLALYVLAIFLAGGGSGAFITWQVCRRMPVVSRPPAE
                     IGARLRARFQSRLALTPDQARKIDPMIDQATRRVETIRQETASQVFANVSNLHEQVLM
                     VLTPEQKATFEELDRERREHLRQKFGPATNSP"
     gene            complement(831..1127)
                     /locus_tag="VIL39_03965"
     CDS             complement(831..1127)
                     /locus_tag="VIL39_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480612.1"
                     /translation="MKPTDQTLQRLLQAAAKAAPEAVDAPPLGLETRVLANWRAAGMD
                     DDSVSVFAFFRRAVLSAGLVLMLSAAWSLTQSSGNLTGDEAAQLDYNIQMSLNP"
     gene            complement(1252..1842)
                     /locus_tag="VIL39_03970"
     CDS             complement(1252..1842)
                     /locus_tag="VIL39_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5480613.1"
                     /translation="MPEPSFDVAACVERVRRRDEDAARALFRHLYPLVARLVRSHLPR
                     RTSQEDLVQTIFMKVFANLDRFSGAVPLEHWVSRIAINTCLNQLAAEKARPELRWADL
                     SEEQTQALEAATAGPEELDPLKNLAAREIVEKLLAQLAPADRLVLTLLHLEGRSVDEV
                     RQITGWNVPVIKVRAFRARRKLRKHFETLMAEKIRN"
     gene            1896..2237
                     /locus_tag="VIL39_03975"
     CDS             1896..2237
                     /locus_tag="VIL39_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480614.1"
                     /translation="MVTGFFKTLIYQHRRSFGGLDHVLELRFAPALLLLALCRAGRYL
                     SAMKVAEIRDLVERQPFRPFGVRLNNGAHYTFKDSRDVGAPKDYRLLIYFGKSEAVRI
                     DTDSIVEIFER"
     gene            complement(2386..>2803)
                     /locus_tag="VIL39_03980"
     CDS             complement(2386..>2803)
                     /locus_tag="VIL39_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5480615.1"
                     /translation="KLIAKGLMPANGDVLLDTSVVIPYFKGDRAVHTQIQASAKLHLP
                     QTVLGELICGAHLSTNPARTLAEVQNFLKAVVVLSPGVTTAEHYGRIRADLAKAGTPI
                     PENDIWIAALALEHDLPLAARDAHFDRISGLQVLKW"
BASE COUNT          509 a          911 c          800 g          583 t
ORIGIN      
        1 atctgcgcca gcaaatcccc cgtgcgcacg tcctggccct cggtaaaggc gattttctgc
       61 acctgcccat ccacccgggc gtggatggtc accgtgttaa acgcctggac ggtgccactg
      121 cctcccagat agatcggcac atccttttcg gcgaccgtgc cggccacgac cggcactggc
      181 ggcagctcca tccggccggc gcccggccct ttcttgtcgg cgcccgcagc gcgactccgc
      241 gccagccaca ctgctccggc caccaccagc aggcacagca caacgatgaa cgccattttc
      301 ccgccggagg aattaggttt cggggtctcg ctgccgggca acggcggact ggcattcgca
      361 ggttggcgtg aggggggcat agttcgtttg ttggtcatct cgtccatggt tggcatatct
      421 taaaacgcct tacgggctgt tggttgcggg gccgaacttc tgacgcaggt gctcgcgccg
      481 ctcgcgatcc agctcctcaa atgtcgcctt ctgctccggc gtcagcacca tcagcacctg
      541 ctcgtgcagg ttcgagacgt tggcgaagac ctggctcgcc gtttcctggc ggatggtctc
      601 cacccggcgc gtcgcctggt caatcatcgg gtcaatcttc cgcgcctggt ctggcgtcag
      661 cgcgagccgg gactggaacc gggcgcgcag ccgcgccccg atctccgccg gtggccggga
      721 cacgaccggc atgcggcggc acacctgcca ggtgataaac gccccgctgc cgccgcccgc
      781 caggaagatg gccagcacat acaaagcgag cttccttttg ccaagcgagt tcatgggttc
      841 aggctcatct ggatgttgta gtcgagctgg gcggcttcgt cgccagtcaa gttgccgctg
      901 gactgggtga ggctccaggc ggcgctcagc atcagcacga ggccggcgct gagcactgcg
      961 cggcggaaga acgcgaacac ggaaacggaa tcatcgtcca tccccgccgc acgccagttg
     1021 gccagcaccc gggtttccag gcccagcggc ggagcgtcaa cagcctcggg cgccgccttc
     1081 gcggcggcct gtaacaacct ttgcagcgtt tggtcagtcg gtttcatatt tgctctatgc
     1141 aaggctttcc gtgaagactg gcgcaacccg cgcaaactct tagtcctcat gtaacccatg
     1201 taacccatgt aacccatgta actctcttgc ctgcgtcatg gcgtctttgc gttaattccg
     1261 aatcttctcc gccatcagcg tctcgaaatg cttccgcagc ttccgtcgcg cccggaaggc
     1321 gcggaccttg atgacgggca cattccagcc ggtgatttgc cgaacctcgt ccaccgaccg
     1381 cccttcgagg tgcagcaaag tcagcaccag ccgatccgcc ggagcaagct gtgccagcag
     1441 tttctcgaca atctcccgcg ccgccaggtt cttcagcggg tccagctctt ccggcccggc
     1501 cgtggccgcc tccagcgcct gcgtctgctc ctcgctcaag tcggcccagc gcaactccgg
     1561 gcgcgccttc tcggccgcca gttggttgag gcaggtattg atcgcaatcc gcgagaccca
     1621 atgctccagc ggcaccgcgc cggagaaccg gtcaaggttt gcgaatactt tcataaatat
     1681 ggtctgcact aaatcttcct ggctggtgcg gcggggcaga tgcgaccgca ccagcctggc
     1741 caccagcgga tacaaatgcc gaaacagtgc ccgcgcggca tcctcgtccc gccggcggac
     1801 ccgctctacg caggccgcca catcaaaact cggttcaggc atgtcgctca ccggggccat
     1861 catgccataa ggagttatga ccggctagcc caagagtggt tactggtttc ttcaagacct
     1921 taatatacca gcaccgccgc tcgttcggcg gacttgacca cgtcctcgaa cttcgctttg
     1981 ctcctgcttt gctcctgctt gccttatgcc gcgctgggcg ttatctttcc gccatgaaag
     2041 tcgcagaaat cagggatttg gtggaacgcc agccttttcg cccctttggt gtccgcctca
     2101 acaacggtgc gcattacacc ttcaaagatt ccagagatgt cggtgcgcca aaggattacc
     2161 gtctcttgat ttatttcggc aagtccgaag ccgtgcgcat agacaccgat agcattgttg
     2221 aaatcttcga gcggtaaaac cgtcatgatg ggtgcccggt gcttcttacc cccggttaca
     2281 gccgcttcac ccggatatcc ttcagagtct ggcgctgcct ttcacgcaaa cagttggcga
     2341 atctttgatc tttgacttcg cttcctgcaa cttcgcacat agcgtttacc atttgagaac
     2401 ctgcaagccg ctgatgcggt caaaatgagc gtctcttgcc gccagaggga gatcgtgctc
     2461 aagggccagg gcggcaatcc aaatgtcgtt ttcaggaatc ggcgtgccgg ccttggccag
     2521 atcggcacgg atgcggccgt agtgttccgc cgttgtcacc cccggggaaa gcacgaccac
     2581 ggccttgagg aagttttgaa cttcggccag agtcctggct ggattcgtgg agagatgcgc
     2641 gccgcaaatc aattcaccga gaaccgtctg cggaaggtgg agcttcgcgc tggcttggat
     2701 ttgggtgtga acagcccggt cgcccttgaa atagggaatg accaccgagg tgtccaacag
     2761 gacgtcacca ttggccggca tcaacccttt cgcaatgagt ttc
//
LOCUS       DATCGS010000196         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_662174, whole genome shotgun sequence.
ACCESSION   DATCGS010000196 DATCGS010000000
VERSION     DATCGS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662174"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIL39_03985"
     CDS             complement(<1..219)
                     /locus_tag="VIL39_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480616.1"
                     /translation="MSESVIICGRVFRPELVQHLSQMATRQPPPGRNVLAHEVCTHLA
                     WFSPNGRPALSGAKVALRKLSRRGLLSLP"
     gene            736..1740
                     /locus_tag="VIL39_03990"
     CDS             736..1740
                     /locus_tag="VIL39_03990"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5480617.1"
                     /translation="MNRKPHVAVVGATGAVGIEMIKTLEKRNFPVGKLTLLASARSVG
                     KKLKFRGEDVTVKELKKDSFGGIDIALFSAGGAISKEYAPIAAKAGCVVIDNSSAFRM
                     DDKVPLVVPEINAADVKWHKGIIANPNCTTAITLIALYPLHAAFGCKRIFASSYQAVS
                     GTGAKAIAELERQVDQIVKKQPVTREVYPHQIAFNVLPHVDSFLPSGYTKEEMKMENE
                     GRKIMHHPNFRASVTCVRVPVYRAHCVAVSAEFEKPTTVEAALAVLKKAPGLDVIDNP
                     AKCEYPLPLYQSDKYNCAVGRLRKDCALDNGLCFWVSGDQLLKGAALNAVQIAEELMK
                     "
     gene            1874..>2588
                     /locus_tag="VIL39_03995"
     CDS             1874..>2588
                     /locus_tag="VIL39_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5480618.1"
                     /translation="MESQEPIPYESGQNPPASANRASPPAISIRGLTKLYGPLTALND
                     LNLDIHRGETFGFLGLNGAGKTTAIRLLLDLLRPTSGKAFIFGYDCWSEGLEARARIG
                     YLPGELGLYLDLTGLETLDFLAGLNRQPVDKRHRQELCDRLELPRSDLRRRLREYSTG
                     MKRKLGIIQAFQANPPLLILDEPTEGLDPLMQESFYGLLADVKRGGATVFMSSHVLSE
                     VERVCDRIALLRKGEMVLLS"
BASE COUNT          521 a          801 c          780 g          486 t
ORIGIN      
        1 aggcagtgac aacagacccc gacggctgag cttgcgcagc gccaccttgg ccccggaaag
       61 ggcgggccgc ccattgggtg agaaccaagc cagatgggtg cacacttcgt gcgccaagac
      121 attcctgccg ggcggtggct gccgggtcgc catttggctc agatgctgca ccaattcggg
      181 acggaagact cggccacaga taattacaga ctcactcatc cggcgatagt gctcgccgga
      241 aaccgaaatg tctatagcgc ataaatggtt ataaggagca gcgtcctccg gcgcagcaag
      301 ccccgaatgt gctgacgcgc cggatttcat tatatctttg ccgctttcca acattgctgc
      361 gcccgaggac gggcgcactc cggcacgacc ttctcctgct gtcactgacc cattactcga
      421 ccccggtgct ttgggtggat ctgttttctg gtacatgccg cgggatgcgc cacgcaatag
      481 acggccggga ggacgccagt gtcccgtcaa agcggcttcc ccaacggtgg aatagggcct
      541 gcggctggct gggtgctccg gcgcagcgag cctgatgctt cgaaggagtt acgatggggc
      601 ttcgaagggg ttacgatggg gttacgaagg gaatcaaggg aaatcagccc tccgttacac
      661 cctctcgacc gcgttgggga ctcggcgaca cgttgacatg gctggccgcc gctcgctata
      721 ttctgccccc tttatatgaa ccgtaaaccg catgtggctg tagttggtgc gacgggcgcc
      781 gtgggcatcg agatgatcaa gacgctggag aagcgcaatt tcccggtggg gaagttgacg
      841 ctgctcgcct cggcccgttc ggtgggcaag aagctgaagt tccgggggga agacgtcacg
      901 gtcaaggaac tgaagaaaga ctccttcggc ggcattgaca tcgcgctgtt cagcgccggc
      961 ggcgccattt cgaaggagta tgcgcccatc gcggccaagg ccgggtgcgt ggtcattgac
     1021 aactcgagcg ctttccgcat ggacgacaag gtgccgctgg tagtccccga gatcaacgcc
     1081 gcggacgtga agtggcataa gggcatcatc gccaacccga attgcaccac ggccatcacg
     1141 ctcatagcgc tctacccgct gcacgcggcg ttcggctgca agcgcatttt cgcctccagc
     1201 taccaggcgg tttcgggcac gggggccaag gcgattgccg aactggagcg ccaggtggat
     1261 cagatcgtca agaagcagcc ggtgacgcgc gaggtttatc cgcatcaaat cgctttcaac
     1321 gtgttgccgc acgtggattc cttcctgccg tccggttaca caaaggaaga gatgaagatg
     1381 gagaacgaag gccgcaagat catgcaccac ccgaacttcc gggcgagcgt gacctgcgtc
     1441 cgcgtgccag tctatcgggc gcactgcgtg gcagtcagcg ccgagtttga gaagccgacg
     1501 acagttgagg ccgcgctagc cgtgttgaag aaagcgccgg gcctggatgt gatcgacaac
     1561 cccgccaagt gcgagtatcc gctgccgctc taccagtcag acaaatacaa ttgcgccgtc
     1621 ggccgcctcc gcaaggattg cgcgctggat aacggcctgt gcttctgggt cagcggcgat
     1681 caactcctca aaggcgccgc cctcaacgcc gttcagatcg ccgaagagct gatgaagtaa
     1741 tccaagggca ccttcttggc gccctgcaat ccaaggctgc ggcgcaggta tcgtccagca
     1801 gcgtgacacc caggcattag ccctgtcgaa ccactacgct ttgcggaaat atcaactagc
     1861 cgctccattg cccgtggaat ctcaagaacc cattccctat gagtctggcc agaatccgcc
     1921 tgcgagcgcg aaccgcgcgt ccccgcccgc catttcgatt cgcggtctta ccaaactata
     1981 tgggccgctc accgccctga acgatctcaa cctggacatc caccggggcg agacctttgg
     2041 cttcctcggg ctgaatggcg cgggcaagac cacggccatc cgtctcctgc tcgatttgct
     2101 ccggcccacc agcggcaaag cgttcatctt cggttacgac tgttggtccg agggcctcga
     2161 agcccgggcg cgaatcgggt acctccccgg agaactgggg ctttacctcg acctgacggg
     2221 gcttgaaacg ctcgatttcc tcgcgggcct caaccgacag ccggtggaca aacgacaccg
     2281 acaggaactc tgtgaccgat tggaattgcc tcgcagcgat ttgcggcggc ggctgcgtga
     2341 atacagcacc ggcatgaagc gcaaactggg aatcatccag gccttccagg cgaacccgcc
     2401 cctgctcatt ttggacgagc caaccgaggg cctcgatccg ctcatgcagg agtcgtttta
     2461 cggcttgctc gcggacgtca aacgcggcgg cgcgaccgtt ttcatgtcgt cccacgtgct
     2521 atcggaagtc gagcgtgtct gcgaccggat cgcgctcttg cgcaaaggcg aaatggtcct
     2581 cctctcca
//
LOCUS       DATCGS010000197         3755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_562772, whole genome shotgun sequence.
ACCESSION   DATCGS010000197 DATCGS010000000
VERSION     DATCGS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562772"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..645
                     /locus_tag="VIL39_04000"
                     /pseudo
     CDS             1..645
                     /locus_tag="VIL39_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472234.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(750..1904)
                     /locus_tag="VIL39_04005"
     CDS             complement(750..1904)
                     /locus_tag="VIL39_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5480619.1"
                     /translation="MSNGQQPKWDPRSKEVLEDQINAYDGIRHRYQVAFSDYLQWSLF
                     KHKDVVQVLNDHETFSNAVSSHLSVPNGVDPPEHTRYRKMIETYFDSQQMDVFEPVCR
                     RIAVSLVNRLPLRGEVEFMSKIAEDFALQIQCAFMGWPAELHEPLRQWTRKNGDATLA
                     GDREAMAAVALEFDGYIRDLLRVRRDAGSQAPDDVTTRLLRERVGDRLLKDEEIVSIV
                     RNWTVGELATISSCLGILAQYLSKRPELQRRLREQLSLLAPAIDEILRIHAPLISNRR
                     VTTKAVEIGGNHLEIGARITLIWASANRDEAVFGDPDEFRLDRDPSKNLLYGAGVHVC
                     PGAPLARLELRIIMEELLGRTRHIATVPGKHPTRAVYPASGFSSLPLWIE"
     gene            complement(2272..2745)
                     /locus_tag="VIL39_04010"
     CDS             complement(2272..2745)
                     /locus_tag="VIL39_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5480620.1"
                     /translation="MDQDVAKHDGLRRSAPDLALRTQVGVPDSLMTQVMEGVLDIAVI
                     YAPQHRPGLKIELLFEEKLVLVTTGPDASRTGLNPETAVRSDYVYVDVASSRSAGPVT
                     SGCAVCSRTLRKESSTECLARRSSPAYAVYGTNADETVLRTALEGLRAVAGEEQS"
     gene            complement(2720..3151)
                     /locus_tag="VIL39_04015"
     CDS             complement(2720..3151)
                     /locus_tag="VIL39_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5480621.1"
                     /translation="MEDRFQLAEWILDQVADAVIYANRSGAIIRWNCASAALFGFSAE
                     EAIGQRLDLIIPEHLQAAHWRGFEAAMTKGAMKLQGRPTLTRALHKNGRKLYVEMTFA
                     VVKGDAEGEVLGSVAMARDVTDRVERERSAARVSGSGRREA"
     gene            complement(3254..3586)
                     /locus_tag="VIL39_04020"
                     /pseudo
     CDS             complement(3254..3586)
                     /locus_tag="VIL39_04020"
                     /inference="COORDINATES: protein motif:HMM:NF040453.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6522 family protein"
BASE COUNT          747 a         1207 c         1063 g          738 t
ORIGIN      
        1 gttcaagcgc gccaggcgag agctcaaatt cctgctaacc cgtctcggcc gcatcattcg
       61 cgacatccgc cgcaagatcg agggcaaccc ggcgctggag gaacgcttcg ctccgctgct
      121 cgatctcgcc gcagcgccag cgcagaccca aggtctattc tctgcatgcg cccgaggtcg
      181 aatgtatcgg caaaggcaag gcccgcgcgc cttacgagtt cggctgcaaa cccgtcaccg
      241 ccccacggca atccgtatga cggccatacg ctcggccccc gtcatcaacg gcctgcaggc
      301 actgaccggc attgagcccc gccgcgtcca catcgacaaa ggatatcgcg gccacaacta
      361 ccccgaccgc ttcaaggtct ggatctccgg ccaggtccgc cgggtcacca aaaccattcg
      421 ccgcgagatg aagcgcaaag ccgccgtcga gccatcatcg gccacctcaa ggccgatcac
      481 cgcatgggcc gaaattacct caagggccgc caaggcgacc gcatcaacgc gatgctcgcc
      541 gccgcaggat tcaacttcag cctgttgcta cgatggttcc aacggctttt gcgcgccctg
      601 tttgccgccc tctgccgcgc agtcgtctct ccccgcttcg cataaacagg caaaagacta
      661 ctttcttcac ggacgactac gaactcttat gtcattgaaa tcgcatcata gttcaggacc
      721 gttctcgggt ctgcgccgca gatgacggct cattcgatcc acaacggcag cgatgaaaac
      781 ccgctggcgg ggtagactgc cctcgtggga tgcttgcccg gcaccgtcgc gatatgtcgg
      841 gtgcgcccga gtagttcttc catgatgatg cggagttcca gccgtgccaa cggcgctcca
      901 ggacagacgt gcacacccgc gccgtacaag aggttcttcg atgggtcgcg gtcgagccgg
      961 aattcatcgg gatcaccaaa gacggcctcg tcgcggttgg ctgaggccca aatcagggtg
     1021 atccgcgccc cgatctcaag atggttgcca ccaatctcca cagccttcgt tgtgacccgg
     1081 cgattggaga tgagcggtgc gtggatccgc aagatttcgt cgatggcggg ggctaagagc
     1141 gagagctgtt cccgcaagcg ccgctgcagt tccggccgct tagagagata ctgcgccaga
     1201 atacccaggc aggacgagat cgtcgcgagc tccccgaccg tccagttgcg cacgatgctg
     1261 acgatttctt catctttcag cagcctgtcc ccaacccgct cacgaagcag acgagtggtc
     1321 acatcatcgg gcgcttgact gcccgcgtcg cggcggaccc tcaacagatc cctgatgtaa
     1381 ccatcaaact cgagcgctac cgcagccatg gcttccctat ctcccgccaa ggtcgcgtcc
     1441 ccattcttgc gggtccactg gcgcaggggt tcgtgcaact ccgccggcca tcccatgaag
     1501 gcgcactgaa tctgaagggc aaagtcctcc gcaattttgc tcatgaactc cacttcgccc
     1561 cgcaacggca ggcgattcac cagactgacc gcaatccttc tgcacaccgg ctcgaacaca
     1621 tccatctgtt gcgagtcgaa ataggtctcg atcatctttc ggtaccgagt gtgctccggg
     1681 ggatccacgc cattaggcac agaaaggtgg cttgagaccg cattactgaa agtttcatga
     1741 tcgttcagga cttgcacaac gtctttgtgc ttgaacaacg accattgcag atagtcactg
     1801 aatgctacct gatagcgatg acgaataccg tcataggcgt tgatctgatc ctcaagaact
     1861 tccttggacc tggggtccca cttgggttgc tggccattac tcatgagaat cctcagcttg
     1921 gtgcaaggcg aaaagcaggt cagcccgaga ccccgacctg tctagtcgga tgtggtgtcc
     1981 tgtaggtcag tcactccatg gaatgaatga catggatcaa agtgttgtaa cacaacccat
     2041 caatccggat gatgcggtgc gcggcgggtc tcccagcgcg ttgctggcgg gcacgccctc
     2101 atcggccacg tcctcagcgc tgatgccatc ccggtggcga cgccggatca tcaagcgcct
     2161 cgcgggcgag ggccatgtca gctctgggta ccagcggcca cctacgcgtg atgtctgctt
     2221 tgcccgccga aaacggatat tcgccggtgc ggtcggcagg tcagcctcgg cttaggactg
     2281 ttcttcgccc gccacggcac gtagcccctc cagcgctgtg cgcagtacag tctcgtccgc
     2341 attcgtaccg tagactgcat aggccggaga actccggcgc gccaagcact cggtagagct
     2401 ttcctttctc aaggtacggc tgcacaccgc gcatccggaa gtaaccggtc ccgccgacct
     2461 cgaggatgca acatcgacgt agacgtaatc ggaacggacg gcggtctcag gattgaggcc
     2521 cgtgcgactg gcatcaggac cggtcgtgac cagcaccagc ttctcttcga acagcagctc
     2581 tatcttcagc cccgggcgat gctgcggcgc gtatatgacg gcaatatcga gcacgccctc
     2641 catcacctgt gtcatcaggc tgtcgggcac gccgacttgc gtccgcagcg caagatcggg
     2701 ggccgagcgc cgcaagccgt catgcttcgc gacgtcctga tccactgacg cgggctgcgg
     2761 agcgctctcg ctcaacgcgg tcggtgacgt ctcgcgccat ggcaactgag ccaagcacct
     2821 cgccttcggc gtcgcccttg acgaccgcga atgtcatctc gacgtagagt ttgcgcccgt
     2881 tcttgtgcag cgcgcgggtc agcgttgggc ggccttgaag cttcatcgcg ccctttgtca
     2941 tcgctgcttc gaagccgcgc cagtgtgcgg cttgcagatg ctcgggaatg atcagatcga
     3001 ggcgttggcc gatcgcctcc tccgcggaga agccgaacaa tgcggcagag gcgcagttcc
     3061 agcggatgat cgcgcccgag cggtttgcat agatgacggc atcggcgact tgatcgagaa
     3121 tccactcagc caattggaac cggtcttcca taggcatgcc tttcgggtct gagaccatcg
     3181 gctcgctcgc gcacaatcgg cgggtgagcg cagtgcgtgg accgagcgca agtcgagttt
     3241 tatccccgcc tcctcagcgg cccggccgat gcagggcgtt cggaaccgga cgatctccga
     3301 aatcgatggt cgacctgcga atgatccgtc ctttcggatc catactaagg cgcgcgcgac
     3361 gattgcgata gaagaacgtg agccggaatt cgccttcgtt ctcgtcgacc taatgttcgc
     3421 agaagcctgt gatcggccgt tcacgcatca ggatcggcac gcgcgatgcc ggtatgtcca
     3481 gaagctcgcc cagctgcgca gcgtttacta caaatgtgcc gtcgctgatc tcgatatcag
     3541 gtgcggacga ctctggagtt ccggttcccc ggcagctatc gaccatcaaa attcctcttg
     3601 ccgagacgcc taaaagatgt atttattata catcttttat ggtccaggag tcaatccatg
     3661 cgccatcaat ccggactgga gagctaggca aaattacaga cgccgccgga agcgattctc
     3721 cagcgtgaat ttccgcccgg gacgtcgggg ccccg
//
LOCUS       DATCGS010000198         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_67913, whole genome shotgun sequence.
ACCESSION   DATCGS010000198 DATCGS010000000
VERSION     DATCGS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67913"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..806)
                     /locus_tag="VIL39_04025"
     CDS             complement(441..806)
                     /locus_tag="VIL39_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY5480622.1"
                     /translation="MKLLLDQGTPRSAATILRRAGFDAVHTGETGLAEAEDSEILRRA
                     SNDNRIVVTLDADFHTQLALTQAREPSVIRIRIEGLRAAEFSGLLQKVLNQCADDLKA
                     GAMISVNDCQIRVRRLPVS"
     gene            complement(803..1033)
                     /locus_tag="VIL39_04030"
     CDS             complement(803..1033)
                     /locus_tag="VIL39_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5480623.1"
                     /translation="MTFKRITCDPKRMNGQSCVRQLRLTVRRVVEAVATYPDRRDLQR
                     EFPELEAEDIREALAYAAANLDDKVIELATAG"
     gene            complement(1797..>3066)
                     /locus_tag="VIL39_04035"
     CDS             complement(1797..>3066)
                     /locus_tag="VIL39_04035"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY5480624.1"
                     /translation="TFEPKQIALRHTQTDAVLGIAVPTEQQIEYLQRLGLEIATAADI
                     GPATGMRMPHTTVFRVPSFRVDLKREIDLIEEVARLYGVDKIPATAPRGAIGTNPYDA
                     VHDQLADARRILTGLGLFEAQGQTLISDAAAKLMAGDAMVPLSNPLSSDMNVLRPSLL
                     PGLLDALRHNLSHKTYDVAIFEVGRVFTVAQASPPASSPGVPPGATSTTREERRVAIA
                     LTGQRASLFWSGNDREAKFDIYDLKGLLEEFLEQFGLRGLNYARRQNSTALLLESATV
                     HLGRFQLGEFGQLLPPLARQYDLRDAVLLAELNLDLLLARRNTTKSFRPLPAFPAIRR
                     DVAMLVPEATTHEAVLQVVKQAKPANLETTDLFDVFRGKNVPPGQKSMAYAFTYRRAE
                     RTLTDADVNAAHEKLVAQLKQRLQAVVRES"
BASE COUNT          609 a          858 c         1023 g          576 t
ORIGIN      
        1 agcagaagag gtatcatgtg cccttggaag gcgcgaaggt gtttatccgg gtgtggcagc
       61 aggtgaacgg gtgggagaac cagttgggga tgttccaggg cagcgccttc gtcccggccg
      121 ccgggggcag gtgggcggca aaaaaccgcc atcagtagag gaccaataga ggaccaatag
      181 aggaacccaa tagagagtag cctcgtagca gcacccttga cacgccgtag tggcgccgtt
      241 gaccaacgag gctagccggg cagcagtttc atccagccgg tgatagagac acgaattcca
      301 cgaattgacc cgaaggaagt agccgccggc ggcgagcagg tcgggccagt gggtggaaga
      361 ttcgttcgcc ggccaaaacg tgctcgaagg cactacgcat aatgacgggg gtggttcgct
      421 gtccgcctcg tgcgaccgct ttatgaaacc ggcagacgac ggacgcggat ttgacaatcg
      481 ttcacggaaa tcatcgcgcc ggccttcaaa tcatccgcgc attggttcaa aaccttttgg
      541 agcagtccgc tgaattccgc cgcgcgcaat ccttcaatgc ggatgcggat caccgacggc
      601 tcgcgggctt gcgtcagggc aagctgcgtg tggaaatcgg cgtccagtgt cacaacgatg
      661 cggttgtcat ttgaggcgcg gcggaggatt tcagaatctt ccgcctcggc caggccggtt
      721 tcgcccgtgt gaaccgcatc aaaacctgcg cgacgaagaa tcgttgccgc cgaacgcgga
      781 gttccctggt cgagcagcaa tttcatccag ccgtggccag ttcaatcacc ttgtcgtcca
      841 gattggccgc ggcgtaggcg agcgcctcgc ggatgtcttc ggcttccagt tcgggaaact
      901 cgcgctgcaa atcccggcgg tcgggataag tagcgacggc ctccaccaca cggcgcaccg
      961 tcaaacgaag ctggcggacg cacgactggc cgttcatccg tttgggatca caagtgatgc
     1021 gcttgaaagt catggcatta ctgtgcacgg aaacagcgga aaatgcaatg gcgctttgcg
     1081 cgacggcgag atcacgagtt gacacgaagg gggcggctgt tggcgtggag gcggtcgggc
     1141 cagtgggtgg agaattcttt ctccggccaa agcgtgcttc gaggcactat ggcgtcatgc
     1201 gtgatgcgtg gtccgctgtc cgcatcatgc gaccgaacgg ggcaaacacg acattgcgaa
     1261 cgggcgcaga cttgtgtaga gcaacgcata gcggctctcg ctttcttggt aaagattacg
     1321 gttttcttgg cggggggaag gggcggtcgt gtactaccag attgttgttg gaaaacagag
     1381 aaggactgag cgaaaagcgg gtgggcggtc ctttgacgcg ctcttagccc atgggctctg
     1441 ggatcctctc gtaggagtag gcgatcgcgc taagatcgcg acaggcaccg tacagacgaa
     1501 cgtgctgccg gcggcccaac tcgctgtccg cttcgatatc atcaacggca cggcggcgat
     1561 catgaagagc gcacaggctg cggcggcgac gtaggacaga gcagggaaaa ttctgcgatg
     1621 acctaaggat tctgcgcggt gagccaatcc tgaagttcgt ccagcgtctc gacggacgag
     1681 aggacggcgg cggggatttc aacggttccc gttttcatgg taccagttta tcacggtttg
     1741 ccgggaaagg taagagcttt ccctttcatg gagggaggaa attctgttgc ggctccctat
     1801 gactcgcgca cgaccgcctg caagcgctgc ttcaactgcg ccaccagctt ctcgtgcgcc
     1861 gcgttgacgt cggcatcggt gagcgtccgc tcggcgcggc ggtaggtgaa ggcgtaagcc
     1921 atgctcttct ggcccggggg cacattcttg ccccgaaaca cgtcgaagag gtcggtggtt
     1981 tccaggttcg ccggcttcgc ctgcttgacc acctggagga cggcttcgtg cgtggtagcc
     2041 tcgggcacga gcatcgccac gtcgcggcgg atcgcaggga acgccggcag tggccggaat
     2101 gatttcgtgg tgttccgacg cgccagcagc agatcgagat tcagctcagc caacagcacg
     2161 gcgtcacgca aatcatattg ccgggccagc ggcggcagca gttggccgaa ttcgccaagc
     2221 tgaaatctgc cgaggtgaac ggtggcggat tccagcaaga gggcggtgga gttctgccgt
     2281 cgcgcataat tcagcccgcg caggccgaac tgctccagga attcctcgag caagcctttc
     2341 aaatcataga tgtcgaactt cgcttcgcgg tcattgccgc tccagaacag cgaggcgcgc
     2401 tggccggtca aggcgatggc gacgcggcgc tcttcgcggg tggtggaggt tgcgccgggc
     2461 gggacgcccg gggaactcgc aggcggggac gcctgcgcta cagtgaagac gcggccgacc
     2521 tcgaatatgg ccacgtcgta ggtcttgtgg ctgaggttgt ggcgcagggc gtccagcagg
     2581 ccgggcagca aacttggccg gagcacattc atgtcgctgc tgagcgggtt ggagagcggg
     2641 accatggcat ccccggccat caacttggcg gctgcgtccg agattaaggt ttgcccttgc
     2701 gcctcgaaga gccccaggcc cgtcagaatc cggcgggcat ccgccagttg atcgtgaacc
     2761 gcgtcgtagg ggttggtgcc gatggctccg cgcggggcgg tggcggggat cttgtccacc
     2821 ccataaaggc gggcgacttc ctcaatgaga tcaatttcac gcttcaggtc cacgcggaac
     2881 gaaggcacgc ggaaaacggt cgtgtgcggc atccgcatac ccgtagcggg accaatgtca
     2941 gcagccgtag cgatctccag tccgagtcgc tgcaaatact cgatctgctg ctccgtggga
     3001 acggcgatgc ccagcacggc atccgtttgc gtgtggcgca gggcgatttg ctttggctca
     3061 aaggtc
//
LOCUS       DATCGS010000199         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_562979, whole genome shotgun sequence.
ACCESSION   DATCGS010000199 DATCGS010000000
VERSION     DATCGS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562979"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1362
                     /locus_tag="VIL39_04040"
     CDS             289..1362
                     /locus_tag="VIL39_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5480625.1"
                     /translation="MLRPPQRLSLVAQTVAILQEHIASCRGGEKLASERELCHQLSVS
                     RLTLRAALANLARDGFLHAGQGRRRVIAVTGLGARQPTSSRDVVVLSPLPLQGVDPRV
                     LFWIDELRDALAKENYRLDFVNQRNCYSEHPQRALEELAARLRPAVWLLHLSTHTMQQ
                     WFFAQGLPAVIPGSRYEGVRLPSVDVDYRATCQHAVGRFLARGHRCLALLNPKSSAAG
                     DLESEAGFRQAGAVAGSGVETLIAQHDGTVAGICASLDRLLARTRPPTAFLVSRPTHA
                     LTALGHLIQRGLRFPKEAVFIARDHDSFLEHAVPTVARYQVDAVLFAHKMSRIVLEMA
                     SGGNARSCDYRVTPRLISGETLG"
     gene            1489..1626
                     /locus_tag="VIL39_04045"
     CDS             1489..1626
                     /locus_tag="VIL39_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480626.1"
                     /translation="MLKQSEKLEVLATNRLDDKFDASPAAVGKELFVPGREYLYNLAE
                     K"
     gene            complement(1711..>2595)
                     /locus_tag="VIL39_04050"
     CDS             complement(1711..>2595)
                     /locus_tag="VIL39_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY5480627.1"
                     /translation="LSLARSSLRLSGTKWIRPVGDSARSAGGDGNLLLNVGPMPNGRI
                     APEQADRLKELGAWLAKYGQTIYGTRGGPFKPGTYGASTRKGKTIFLHIRRWPKGALK
                     LPPILAKVLCSRVLTGGTAGLKQGEAGLELTLPLGERQPLYTIIALDLDRSALSLAAL
                     DVQLELSLTTNAKATASNVYHNLPEYGPDKALDGRDDTRWATDEGTTSVWLEVDLGKP
                     TEFSHAVIVQAFPELKRIHRLAIEYFEDGYWKTSFRGQDPGERLDARFEPVTAQRVRL
                     NLLDATGGPTIWEFQLLK"
BASE COUNT          464 a          813 c          794 g          524 t
ORIGIN      
        1 cgatgatcgc gatgacgacc agtagttcga tcagcgtaaa gcccttgggg cattcagttc
       61 ggcatttcat ttttctctgc gagtggcggt gacgccggtt ggaccggtgt tacccccgtc
      121 tctttcgctt attaacgcac caactaacct cccgcgcttg gattcgttaa ccataatttg
      181 atcgggttca acttattcca actgtaggtc gtgcccacac caattaacaa gcggcttgca
      241 cttgtaatat ttgtggacca catgcagccg aggttgtatc ttaaagccat gctccgtcca
      301 ccccaacgcc tgtcgctcgt ggcccaaacg gtcgccattc tccaggagca cattgcgtcg
      361 tgtcgtggcg gcgagaagct ggccagcgaa cgggagcttt gccaccagtt gagcgtcagc
      421 cgtctcacgt tgcgggccgc gctggccaat ctggcgcgcg atggattcct ccatgccggt
      481 caaggacgcc gccgggtaat cgcagtgacc gggctgggag cccgccaacc cacctcgagc
      541 cgtgacgtgg tcgtgctctc accgctcccg ctgcaaggcg tcgacccgcg cgtgcttttc
      601 tggatagatg aactgcgcga cgcgctcgcg aaagagaatt accggctcga tttcgtgaac
      661 cagcggaact gctattcgga acacccacag cgcgcgctcg aggaactggc cgcgcggcta
      721 cgtccggccg tctggctgct ccacctttcc acacacacga tgcagcaatg gttttttgcc
      781 caaggacttc cggcggtgat tcctggctcg cgctacgaag gcgtgcggct gccgtcagtg
      841 gatgtggact atcgggccac ctgccagcac gcggttggcc gattcctcgc cagaggccat
      901 cgttgcctcg cgctgctcaa tccgaagtca tcggctgctg gggacctcga gagcgaagcg
      961 ggctttcgtc aggccggagc cgttgccgga tcgggcgtgg agacgctgat cgcgcagcac
     1021 gacggcaccg ttgctgggat ttgcgcgagc ttggatcgct tgctggcgcg cacgcggccc
     1081 ccaacggctt tcttggtgtc gcgaccgacc catgcgctca cggctttggg ccatttgatc
     1141 caacggggcc tgcgcttccc aaaggaggcg gtgttcatcg cgcgcgacca cgattcattt
     1201 ctcgaacacg ccgtgccaac ggtggcccgc tatcaagtcg atgccgtctt gttcgcgcat
     1261 aagatgtccc gtatcgtgct ggaaatggcc tccggtggca acgcgcgttc atgcgattac
     1321 cgcgtcaccc cccgtctgat ttcaggagaa acccttggtt aagcaacgcc cgcgacaggg
     1381 ggtcgggttc agcggaaagc gattcgttca tggcggtggc ctcctgttcg cgggcacgcc
     1441 ggcgcagccg gcagaggtta tcttgcgggc agaaatggcg tcgccgtcgt gctcaagcag
     1501 tccgagaagc tggaagtgct ggccacgaat cggctcgacg acaagttcga tgcctcaccc
     1561 gccgccgtcg gcaaggaact gttcgtgccc ggacgcgagt acctgtataa cctcgccgaa
     1621 aagtagcatg ggcggcagaa ttggggaagg gcatcggcgc acgagaagtg gtttgctcta
     1681 gccgacaggg gacacaggac tcgatcactt tcacttcagg agctggaatt cccagatggt
     1741 cggcccgccc gttgcgtcga gcaggttcag ccgcacgcgc tgcgcagtca cgggttcaaa
     1801 cctcgcatca agcctctcgc cggggtcctg gccccggaaa ctcgtcttcc agtagccgtc
     1861 ctcgaagtat tcgatggcca gcctgtggat gcgtttcagt tccggaaagg cctgcacgat
     1921 aacggcatgg ctgaattctg tcggcttgcc aagatcgacc tccaaccaga cggaagtggt
     1981 gccttcgtcg gttgcccagc gcgtatcgtc cctgccgtcc agcgccttgt cggggccgta
     2041 ctcaggcaag ttgtgatata cattcgaagc ggtcgccttg gcgttggtgg tcagcgacag
     2101 ctccagctgc acgtccaggg cggcgaggct gagggccgaa cgatcgagat cgagcgcaat
     2161 aatggtgtac aatggttgcc gttcgcctag gggaagagta agttccagcc cagcctcgcc
     2221 ctgttttagt ccagccgtcc cgccggtcag tacgcggctg cacaggacct tcgccagtat
     2281 cggcggcaac ttgagcgcgc ctttaggcca gcgccgaata tgcaggaaga tggtcttgcc
     2341 tttgcgggtc gaggcgccgt aggtgcccgg cttgaagggc ccgccccggg tgccatagat
     2401 ggtctggccg tatttggcga gccaggcacc caactccttg agccggtcgg cctgctcggg
     2461 cgcaattcga ccgttgggca tcggccccac gttcagcaac agattgccat ccccgcccgc
     2521 gctcctggcc gagtcgccca ccggcctaat ccacttcgtc ccggacagcc tcaaggacga
     2581 gcgggccaag gacaa
//
LOCUS       DATCGS010000200         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_67961, whole genome shotgun sequence.
ACCESSION   DATCGS010000200 DATCGS010000000
VERSION     DATCGS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67961"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..220
                     /locus_tag="VIL39_04055"
     CDS             92..220
                     /locus_tag="VIL39_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5480628.1"
                     /translation="MPLEEADGGTIFLDEVGSMPAMLLGISIAMLYRRLDGEDKEP"
     gene            335..1300
                     /locus_tag="VIL39_04060"
     CDS             335..1300
                     /locus_tag="VIL39_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEY5480629.1"
                     /translation="MPLTHTKDLFAKALKGKYALGAFNVNNMELLQAIVEACEEEKAP
                     VMLQISKGARQYANPVYLKKLIEAAVSLSNIPIAVHLDHGDSFELCKDCIDDGFTSVM
                     IDASHEPFEKNIEICRKVVEYAHKHNGVVEGELGQLVGAQFDEGEAGGSYSQAGHYTN
                     PGQAVEFVKQSGVDSLAVAIGNSHGAYKFKGEQHLDLERLKAIKKALMDAGLGDYPLV
                     LHGASSVPKDLVEEINKYGGKLGTDAAGVPEADIEVARRIGCAKVNIDTDLRLAMTAA
                     IRKAFGENPKEFDPRKYLGPARTKVKELVRHKVRNVLCCAGHAFD"
     gene            complement(1428..3011)
                     /locus_tag="VIL39_04065"
     CDS             complement(1428..3011)
                     /locus_tag="VIL39_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5480630.1"
                     /translation="MLPAKLFRRKSIDRLVGEAIKPEHQLKRALGPVQLTLLGVGAII
                     GAGIFSTVGTAAAGGGEHIGAGPALVVSFTFVAIACGFAALCYSEFASMVPVSGSAYT
                     YAYATLGELIAWIIGWDLILEYAVGNVAVAISWSDYFQSLLAGFKVVWPVWLGTDYRS
                     AVQAAHKLAEAKAAGVDLSTLSDAVVRGSQAWTLAPRMAGIPLIFNLPAVLIVAFITW
                     VLVRGIRESAGFNSTMVILKLAIVAFFVAVGACYVKPANWTPFAPHGLAGISSAGASI
                     FFAYIGFDAVSTAAEETRNPQRNVPIGIIASLILCTIIYIAVALVLTGMVKWDQFTGV
                     ADPLAKAFSVRGMNWTAGIISFGAVFATTSVLIVFQLGQPRIFFSMARDGLLPAWAAK
                     VHPKYRTPHVTTILTGVFVAVFAAVTNIDEVVDLCNIGTLFAFVLVAAGILVLRRQDP
                     DRRRPFRTPFVPWVPLLAILTCGYLMVVQPRVTWVRFVVWLVVGLVLYFCYGFRRSRL
                     GTAQAQTPKAPGPQGTKPE"
BASE COUNT          710 a          911 c         1042 g          544 t
ORIGIN      
        1 aagggctacc aattcgtgcg cctggacgag ttgctggagc cgaaggtcag taagacatac
       61 ccgaggagga tgaacggcag cctcgtaacc gatgcctctc gaggaggcgg atgggggcac
      121 gatcttcctg gatgaagtgg gttcgatgcc ggcgatgttg ctgggcatca gcatcgcgat
      181 gctgtatcgg cggctggacg gcgaggacaa ggagccgtag ccaaagttgg tcatcttttt
      241 tgctttccta gcccgtggag ttgcctatta tgccggctgg tcgcagttat tgcggccgcc
      301 aggtgagtga ttcattaata ggaaaataaa gattatgcca ctgacacata cgaaagattt
      361 gttcgccaag gccctgaagg gcaaatacgc gctgggcgct tttaacgtca acaacatgga
      421 gctcctccag gcgatcgttg aggcgtgcga agaggagaag gcgccggtga tgctccagat
      481 ttccaagggc gcgcgccaat acgccaatcc ggtgtatctc aagaagctga ttgaggcggc
      541 ggtgagcctt tccaacatcc ccatcgcggt ccacctcgac cacggggatt ctttcgagct
      601 gtgcaaggac tgcattgacg acgggttcac cagcgtgatg attgacgcca gccacgagcc
      661 attcgagaag aacatcgaga tctgccgcaa ggtggtggaa tacgcgcaca agcataatgg
      721 cgtcgtggag ggcgagctgg gccagttggt gggggcgcag ttcgatgaag gcgaagcggg
      781 gggcagctac tctcaggcgg ggcactacac caaccccggg caggcagtcg aattcgtgaa
      841 gcagtccggg gtggattcgc tggcggtcgc catcggcaac tctcacgggg cgtataagtt
      901 taaaggggaa cagcacctcg acctcgagcg gctcaaagcc atcaagaagg cgctcatgga
      961 cgcgggcctg ggcgattatc cgctggtgct ccacggggct tcgagcgtgc ccaaagacct
     1021 ggtggaggaa atcaacaagt atggcggcaa gctggggacg gatgccgccg gcgtcccgga
     1081 ggcagatatc gaggtcgccc gacgcatcgg ctgcgccaag gtcaatattg atacggatct
     1141 gcgcctggcg atgacggcgg ctatccggaa ggcttttggg gagaacccga aggagttcga
     1201 tccgcgcaag tatcttgggc cggcccgcac gaaggtcaaa gagctggtgc ggcacaaggt
     1261 gcgcaacgtc ctctgctgtg cgggacatgc ctttgattag cgggtaaccg caagagccaa
     1321 tcgcgagcac tgcttctttt gattccgctg ctggccggat gcgctcagtc cttacggatg
     1381 agaggatcca gggaagcaac gatctggtct ggcgggaccg ctccccctca ttcgggtttc
     1441 gtcccttgtg gccccggggc tttgggtgtt tgcgcctggg cggttcccaa ccggctccgg
     1501 cggaagccgt agcagaagta aaggaccaga cccacgacca gccaaactac gaaccgtacc
     1561 caggtcacgc ggggctgaac taccatcagg tatccgcagg ttagaatcgc cagcagcggc
     1621 acccagggaa cgaacggggt gcggaatggg cgccgacggt cagggtcctg gcgtcggagc
     1681 acgaggatgc ccgccgcgac caggacaaag gcgaagagcg tgccgatgtt gcacaagtca
     1741 accacttcat caatgttcgt caccgcagcg aagacggcga caaagacgcc ggtcaagatg
     1801 gtcgtgacgt gcggcgtgcg gtatttggga tggactttgg cggcccaggc cggaaggagg
     1861 ccatcgcggg ccatcgagaa gaagatgcgc ggctggccga gctgaaacac gatcaggacg
     1921 gaggtggtgg cgaacaccgc gccgaaggag atgatgcccg ccgtccaatt catgccgcgg
     1981 accgagaacg ctttggccag cgggtccgcc acaccggtga actggtccca tttgaccatg
     2041 ccggtgagca ccagggctac ggcgatgtaa atgatggtgc agaggattaa actggcgatg
     2101 atgccgatgg gcacgttgcg ctgcggattc cgggtctctt cggcggcggt ggatacggcg
     2161 tcaaagccaa tgtaggcgaa gaagatgctc gccccggcgc tggaaatgcc ggccagcccg
     2221 tgcggcgcaa acggcgtcca gttcgccggc ttaacgtagc aagcgccgac cgcgacgaag
     2281 aacgcaacaa tcgccagttt caagatcacc atagtcgagt taaagcctgc gctctcgcga
     2341 atgccccgga ccagcaccca ggtaatgaat gccacaatga gcactgccgg caggttgaag
     2401 atgagcggaa tgcctgccat gcgcggggcc agggtccagg cctggctccc gcggacgacc
     2461 gcgtcgctta gcgtgctcaa gtcaaccccg gcggctttgg cttcggccag cttgtgggcg
     2521 gcctgcacgg cggagcgata gtctgtgccc agccacaccg gccagaccac cttgaacccg
     2581 gccagcaggc tctggaaata atccgaccag gaaatggcga cggccacatt gccgacggca
     2641 tattcaagga tcagatccca gccaatgatc cacgcgatca gttccccgag tgtcgcatac
     2701 gcgtaagtgt aagctgagcc ggaaaccggc accatggagg caaactccga atagcacagt
     2761 gcggcaaagc cacaggcaat ggccacgaac gtaaacgaca ccaccaacgc cgggcccgcg
     2821 ccgatatgtt ctccgccgcc ggccgccgcg gtgccgacgg tggaaaagat cccagcgccg
     2881 atgatcgcgc ccacgcccag cagagtcagt tgcaccggcc cgagcgctcg tttgagctgg
     2941 tgctccggtt tgatggcctc gccgaccagt cggtcgatgg attttctacg aaagagcttg
     3001 gccggcagca tgggaggaaa tgaatccgaa cgccggaaga cgacaccctt gtgcagcagg
     3061 gtggcgattc gcgttgcggc aagcgtgccg agaggcctgg tttctggctg gcattaccga
     3121 gagcagattt cacaaccgcc ccgaacgcaa gcagaaactg aacaactccg ccccaattca
     3181 gggccaattc atgaagtgca aaaggca
//
LOCUS       DATCGS010000201         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_117814, whole genome shotgun sequence.
ACCESSION   DATCGS010000201 DATCGS010000000
VERSION     DATCGS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117814"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VIL39_04070"
     CDS             complement(<1..326)
                     /locus_tag="VIL39_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480631.1"
                     /translation="MLVVCGLLAEAACAETFKLTNGDTLTGELLAGSASDVGVQIKVG
                     EGKYERVLWASFSQEDLKKFAKVQKLEPLVEPFIEITQEERIKKTEPNIKQPPRLERP
                     AAQSLIG"
     gene            complement(427..1815)
                     /locus_tag="VIL39_04075"
     CDS             complement(427..1815)
                     /locus_tag="VIL39_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY5480632.1"
                     /translation="MRDWEQATWAAGQQEAEVIRRVGRCVAHRALQLTQLADLILILA
                     GKGHNGEDARCAREHLADRQVEVLNVTEPTADLPKLDALLALRPALVVDGLFGIGLNR
                     PLGPEWVSFIEHVNATRLPVLAVDVPSGLNGDTGEPQGVAIEASVTLTVGAPKTGLLQ
                     QVAWPFVGRLEVAADVGLAPCPHQSEGQWTLPEDFTGYPPPRMAATHKGSYGHLAIVA
                     GSLGYHGAAVLASRGAQRAQPGLITLHTLEAVYHAVAPQLDAVMVSAWLPHGKLSNSH
                     DAFLLGPGLAAAGMPDQMKLLTRHLWRDSLVPVVVDASALDWVPLDPVPKNALRVMTP
                     HPGEAARLLLSTPQQVQANRLSALRNISRRFGNAWVVLKGHQTLIGRSSGEVYVNPSG
                     NPHLAQGGSGDVLSGYLAGLLAQPALRADPLKTIRYAVWQHGAAADRLQAMRPNWVVE
                     DLLETVGAVKSR"
BASE COUNT          393 a          718 c          605 g          375 t
ORIGIN      
        1 ccgatcaaag actgggccgc cggccgttcc aggcgtggtg gttgtttgat gttgggctcg
       61 gtcttcttga ttctctcctc ctgcgtaatc tcgataaacg gttcgaccag cggctccagt
      121 ttctggacct tggcgaactt cttcaggtcc tcctgcgaga agctggccca caacacgcgc
      181 tcatacttgc cttccccgac cttgatctgc acccccacgt cactggctga gcctgccaac
      241 agttcacctg tgagagtgtc cccgttggtg agcttgaacg tttccgcgca ggcggcttca
      301 gccagcaacc cacaaaccac caacacggca attaaacaaa ttctgcgcac catgaaagtg
      361 ttcaataaca gacggccctt gccaaaggaa gcacaattgc gaggcgaaga gcccctcgat
      421 cacgggctac cggctcttga cggcccccac ggtttcaagc aaatcctcca ccacccagtt
      481 agggcgcatg gcttgcagcc gatctgccgc cgcgccgtgc tgccacacgg catagcgaat
      541 cgtcttgagc ggatcagccc gcagcgcggg ttgggccagc aaaccggcga gatacccgga
      601 cagcacatcg ccgctgccgc cttgggccag gtgcggattg cccgacggat tcacataaac
      661 ctcacccgag cttcggccaa tgagcgtttg atggcccttg agcaccaccc aggcattgcc
      721 gaatcgccgc gaaatgttgc gcagcgcact cagccggttg gcttgcactt gctgcggggt
      781 tgagagcaag agccgcgctg cttccccggg gtgcggagtc atcacgcgca gggcgttttt
      841 ggggaccggg tccagcggca cccagtccag cgcgctggca tccaccacga ccggcacaag
      901 ggagtcgcgc caaagatgcc gcgtcagcaa cttcatctgg tcgggcatgc cagccgccgc
      961 cagccccggc cccagcagga acgcgtcatg cgagttggac agtttgccat gcggcagcca
     1021 ggctgaaacc atcaccgcgt ccagttgcgg ggcaacggca tgataaactg cttccaaagt
     1081 atggagggtg attaggccgg gctgcgcccg ttgcgcgccg cgtgacgcca gcaccgccgc
     1141 gccgtgataa cccagactcc cggccacaat ggccagatgg ccatagctcc ctttgtgcgt
     1201 cgccgccatc cggggaggcg ggtaaccggt gaaatcctcc ggcaaggtcc actgcccctc
     1261 gctctgatgc gggcaaggcg ccaggccaac gtccgcggcg acttcgagcc gcccgacaaa
     1321 cggccatgcc acttgctgca gcaacccggt cttgggtgcg cccactgtca gggtcaccga
     1381 ggcttcgatc gccacccctt gcggttcacc ggtgtcgccg ttcaaaccgg acggcacatc
     1441 caccgccagc actggcaggc gcgttgcatt aacgtgctca atgaagctga cccactccgg
     1501 cccgagcggc cggttcaagc cgatgccgaa caagccatcc accaccagcg ccggacgcaa
     1561 ggcgagcagc gcgtccagct tcggcaggtc ggctgttggt tccgtcacgt tcagcacctc
     1621 gacctggcgg tcggccaggt gttcacgggc gcaacgggcg tcctccccat tgtggccttt
     1681 gcccgcgaga atcaggatca ggtccgcgag ctgagtcaac tggagggccc ggtgggcaac
     1741 acatctcccg acgcggcgaa tgacttccgc ttcctgctga ccggcggccc acgtggcctg
     1801 ttcccagtcg cgcatttgtg caatgctaat tatcggcact ggcatggcgc gggaagatat
     1861 accctcccgg ccgcaacgaa agcggaattg ccagaaatgc cgtaatgggt cagtgacagc
     1921 gggagaaggt cgtggcggag tgcgcccatc ctcgctgcac cccggcccca gtcagccttg
     1981 tcggcagtgg tacgtgcaaa atgcgtcccc gccgtcccgc tttcctccaa gcctttcgcc
     2041 catatcccat agattctagc cttgccaatc cgctcccatt cccctcgcac t
//
LOCUS       DATCGS010000202         3649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_265421, whole genome shotgun sequence.
ACCESSION   DATCGS010000202 DATCGS010000000
VERSION     DATCGS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265421"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VIL39_04080"
     CDS             <1..187
                     /locus_tag="VIL39_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480633.1"
                     /translation="AVNAERRRRLTRAALDYLRLLRNPPVKLRFDIVEVLLQDSAVRE
                     VRHLPNTFAMERPYRYG"
     gene            complement(252..1265)
                     /gene="gmd"
                     /locus_tag="VIL39_04085"
     CDS             complement(252..1265)
                     /gene="gmd"
                     /locus_tag="VIL39_04085"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5480634.1"
                     /translation="MAKKALITGITGQDGSYLAELLLSKGYEVHGLIRRASTFNTGRL
                     DPIYNDPHSAKSRMFLYYGDLSDASVLARLIGKIQPEEVYNLAAQSHVRVSFDSPEYT
                     ADITATGTVRLLEAIRETGIRPRFYQASSSEMYGMVREVPQTETTPFYPRSPYGCAKV
                     FSYWITVNYRESYGLHANNGILFNHESPRRGETFVTRKITRAVAHIQAGLEDKLFLGN
                     LDAKRDWGFAKEYVEAMWLMLQQDKADDYVIATNETHSVREFLEVAFGHAGLDWKKHV
                     ENDPRYYRPAEVDLLIGDYSKAKRQLGWAPKTRFVDLVKLMVDADVDLLRRHRLGQIR
                     VSS"
     gene            complement(1485..>3649)
                     /locus_tag="VIL39_04090"
     CDS             complement(1485..>3649)
                     /locus_tag="VIL39_04090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY5480635.1"
                     /translation="IYFVMQFSKPFASFGIEQDGQRLPADAREGKGRQMKAFVDYPTT
                     AKEAVLVKVGISGTGIEGARKNLKAELPDWNFERVKAAAVKQWKDLLDVAQIETFDPH
                     IRNTFYANLYLCCQAPILYNDVDGTYRGMDHKNHTGANFQNYTIFSLWDTYRAEHPLL
                     TLLQPGRVDDMVQSMLAEYRESGLHTTPIWPLWGNESWCMIGYHSVAVIVDAYLKGFR
                     GFDAEAAYQAMRDTAMQDRNGLKSYKELGYVASTRGGEATSRTIECSFDDWCLARMAE
                     ALGHKEDAALFYQRSANYRNHFDRTVSFFRGRKADGSWRKPFVDNALVGDEYTEADAW
                     QYAFSIQHDVPGMIALYGGDEGFVQRLEAMFNADSTIQTSIPDISGRIGQFSQGDEQC
                     HHVAYLYNYAGAPYKTQERVRQVMDTFYNDTPAGQCGNVDCGQMAAWYVFSALGFYPV
                     NPDSGVYMIGSPVVTKAVLNLDAKKYHGRKFTVIAENNSPKNIYIQSASLNGKPLAQA
                     WLTHEQITSGGTLKLVMGPKPNQDWGRGQEVRPPATMPAGFRYPELPAPFIDKREVLS
                     LPIRVICGNDEPVQGFVPDPNMVSGSTNHKNVKIDTSVTNAAPAAIYQYERYGQDYAY
                     VYEVPKTDRYTVRLHFAEIFNDGEGSRLEDIRLNDQVVLKDFDIFKAAGGMNKAVVKE
                     FKDVAPNDQGNIVIRITAASHSPDKNAKICGIEILKAR"
BASE COUNT          700 a         1106 c         1058 g          785 t
ORIGIN      
        1 ggcggtcaac gcggagcgac gacgccggct tacccgggcc gcgctcgatt acttgcgctt
       61 gttgcgcaac ccgcccgtga agctgcgctt cgacattgtg gaggtgctcc tgcaggacag
      121 cgcggtgcgg gaggtgcggc acttgccgaa caccttcgcc atggaacggc cctatcgcta
      181 cggctgagac agcatatagc gtcgtggaac ttgggttcac gaacgagccc gggaccagag
      241 ctgctcgacc gtcaactgct gaccctgatt tgacccaggc ggtgcctgcg tagcaggtcc
      301 acgtcggcat ccaccatcag ctttaccagg tccacaaacc gggtcttcgg cgcccagccc
      361 agttggcgtt tcgctttgct gtaatcccca atcagcaaat ccacttcggc gggccgatag
      421 tagcgcgggt cattctccac gtgcttcttc caatccagcc cggcgtggcc gaaggccacc
      481 tccaggaact cccgcaccga atgagtctcg ttcgtggcga tgacgtaatc gtccgctttg
      541 tcctgctgca acatcagcca catcgcctcc acgtactctt tcgcgaagcc ccagtcgcgc
      601 ttggcgtcca ggttgcccag aaacagcttg tcctccaacc cagcctggat gtgcgccacc
      661 gcccgcgtga tcttccgcgt cacaaacgtc tccccgcgtc gcggtgactc gtggttgaac
      721 agaatcccgt tgttcgcatg cagcccatag gactcccggt agtttaccgt gatccagtag
      781 gaaaacacct tggcacaccc atacgggctg cgcgggtaaa aaggcgtcgt ctccgtttgc
      841 ggcacctcgc gcaccatccc gtacatctcg ctcgacgacg cttgatagaa ccgcggccgg
      901 atgcctgttt cgcggatcgc ctccagcaac cgcactgtcc ccgtcgctgt gatgtccgcc
      961 gtgtattcgg ggctgtcaaa gctcacccgc acgtgactct gcgccgccag attatacacc
     1021 tcctccggct gaatcttgcc aatcaaccgc gccagcacgc tcgcatcgct cagatcgccg
     1081 taatacagga acatccggct cttggctgag tgcgggtcat tgtagatggg atccaaccgc
     1141 ccggtgttga aagtgctcgc ccgccggatc aagccatgca cctcgtagcc cttggacaag
     1201 agcagttccg ccaggtagga accatcctgc ccggttatgc cagtaatcaa tgccttttta
     1261 gccatacttt gtgagcggca gcttcgggaa cttcaacctc caaggcaagt ctggagaaat
     1321 gcggaagggg gggctagagt agcacggctt tctccccgta atgtccatag accattcccg
     1381 cgatccacga gcccaggaac acgccctcac ccggcggggg ttggccaagg ccataatgag
     1441 aattgctgaa ccgccatggt tgctggttac aggcgaagtg gtcactacct ggctttgagg
     1501 atctcaatgc cgcaaatctt ggcattcttg tccgggctat gcgaggcggc ggtaatccga
     1561 atgacgatgt tgccctggtc gttcggggca acatccttga attccttgac gacggctttg
     1621 ttcatgccgc cggcggcttt gaagatgtcg aagtctttca acaccacctg gtcgttaagg
     1681 cgaatgtctt cgaggcgcga gccttcaccg tcgttgaaga tctcggcgaa atgcaggcgg
     1741 accgtgtatc ggtcggtctt gggcacttca tacacgtagg cataatcctg gccatagcgt
     1801 tcatactgat agatggcggc aggcgccgcg ttggtcacgc tggtgtcaat cttcacgttc
     1861 ttgtggttgg tcgagccgga caccatgttg gggtcgggaa cgaagccttg caccggttca
     1921 tcattgccgc agatcacccg aatcggcagt gagagcactt cccgcttgtc gatgaagggc
     1981 gccggcagtt cgggataacg gaatccggcc ggcatggtgg cgggcggacg cacctcctgg
     2041 ccacggcccc agtcctggtt gggcttgggt cccatcacca gcttgagggt gccgcccgaa
     2101 gtaatctgct cgtgggtcaa ccaggcctgc gccagcggct ttccgttgag cgaggccgac
     2161 tgaatgtaga tgttcttggg actgttgttc tcggcgatga ctgtgaactt gcggccgtgg
     2221 tatttcttcg catcgagatt caggacggcc ttggtcacga ccgggctgcc aatcatatac
     2281 acgccgctgt ccgggtttac cgggtaaaaa cctaacgcgc tgaacacgta ccaggcggcc
     2341 atctggccgc agtccacgtt gccgcattgg ccggcaggcg tatcgttgta aaacgtgtcc
     2401 atcacctggc ggacgcgctc ctgggtcttg tacggggcgc ctgcgtagtt atacaaatag
     2461 gctacgtgat ggcattgctc atcgccctgc gagaactgcc caatgcgccc gctgatgtcc
     2521 gggatgctgg tttggatggt cgaatcggcg ttgaacatcg cttcgagccg ctgcacgaag
     2581 ccttcgtcgc cgccataaag agctatcatg cccggcacgt cgtgttgaat gctaaaggca
     2641 tattgccagg cgtcggcttc ggtgtattca tcgcctacca gggcgttatc aacaaagggc
     2701 ttgcgccacg acccgtcggc ttttcgtccg cggaagaaac tgacggtgcg gtcaaagtgg
     2761 ttgcggtaat tggccgagcg ttgatagaac aacgcggcat cttccttgtg gcccagagcc
     2821 tcagccatgc gggccaggca ccaatcgtcg aaactgcatt caatcgtgcg cgaggtcgcc
     2881 tcgccgccgc gggtggaggc cacatatccc agttccttgt aactcttcag cccgttgcga
     2941 tcctgcatgg ccgtgtcgcg catggcctga taggcggcct cggcatcaaa gcctcggaac
     3001 cccttgagat aggcatccac aatcacggcg acggagtgat acccgatcat gcaccagctt
     3061 tcattccccc acaatggcca gatgggagtg gtgtgcaaac ccgactcccg gtactcggcg
     3121 agcatcgact gcaccatgtc atccacgcgg cccggctgca acagggtcag caacgggtgt
     3181 tcggcgcgat aggtgtccca gagcgagaaa atggtgtagt tctggaagtt ggcgccagtg
     3241 tggtttttgt ggtccatgcc gcgataggtc ccgtccacgt cgttgtacaa tatcggggcc
     3301 tggcagcaga gatagaggtt ggcataaaac gtattgcgga tgtgcgggtc gaacgtttca
     3361 atctgggcca catccagcag gtccttccac tgcttcaccg ccgcggcctt gacgcgctcg
     3421 aaattccagt caggaagttc agccttcaaa ttcttgcgcg ccccttcaat gccggtgccg
     3481 gagatgccca ctttcaccag cacggcttcc ttggcggtgg ttgggtagtc aacaaaggct
     3541 ttcatctgcc gtcccttgcc ttcgcgggcg tcggccggca agcgctgtcc gtcctgctcg
     3601 ataccaaaag aggcaaaggg tttggaaaat tgcatgacga aataaatgg
//
LOCUS       DATCGS010000203         4620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_315909, whole genome shotgun sequence.
ACCESSION   DATCGS010000203 DATCGS010000000
VERSION     DATCGS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315909"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..2204)
                     /locus_tag="VIL39_04095"
     CDS             complement(237..2204)
                     /locus_tag="VIL39_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5480636.1"
                     /translation="MSDRNFSRRRRGMRFRPTGGLGHGQQNPDREATQARADAVGGPP
                     APEKVYEHRHANEIDRAENIAAGLPPEGLPKDAAEAPGSGKDNFPEPNVATPVQLQEQ
                     KPFAPVPIKEQSRGIVETIRNAASSLVKKVQRLIKPVKRIHKEVIINAETLETRVAVS
                     EEGRLEEFTIERTTEERLVGSIFKGRVRNLEDGLKAAFVDIGFEKNAFLHYWDIVPNS
                     FDSGVEIVDRGGRRRDRPKITQKDIPRLYPPGSEIIAQVTKGPIGTKGPRVTTNVVLP
                     GRYLVLLPNSDQSGISRKIENQPERQRLKKILRELAIPDGMGVIMRTVGEGQQKRYFV
                     RDLALLLDEWTQIQERIKSQALATCVFQEPDLIERTVRDFLTEDVERIVVDSQKAYDR
                     MRDMISKISRRSADKVKLYAEPQPIFDRFNITRQLENAFSRQVHLKSGGYIVMDETEA
                     LVAIDVNTGRHKGGKDQEATILKVNLEAADEIARQLRLRNMGGLIVLDFIDMKARRDQ
                     QQVYQRMKDGLRRDKAKTHVLPISQLGLLEMTRQRHSESVDAAVYDDCPYCKGRGKVK
                     SALTMSVEIQRKLSEILKKRSRDESDFQLRIVVHPTILERLRTEDEKHLIEMEKRYFG
                     KLSFRADPALHAEQFKIVNVATNEELASVGG"
     gene            complement(2207..3421)
                     /locus_tag="VIL39_04100"
     CDS             complement(2207..3421)
                     /locus_tag="VIL39_04100"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5480637.1"
                     /translation="MFEVDLQRRESRIDRLQIAALLGLMVLGTLFVYSATMVNESAVS
                     APFCNHTWFRQIIWYALGIGAAAGLCFMDYHTIARWSFVLYGITILLLVAVLIPGIGK
                     THSWGARRWLDLGPFQGQPSEFAKLAFILAQAHFLSRPVGELHELATLWKSLGLMLLP
                     FVLILKEPDLGSALVLLPTGLAILFVAGIPRRYLARLLGGVGLLGALFVADVLFAPPS
                     LQIKLEDYQRRRLLVYFGKEYPLSSGASKMERERSRRQQSDDSLNVRQALISVGSGGL
                     TGKGWRQGTQNALGYLPRPVAHNDFIFSVIAEESGFVGSVIVLTLYGVVLFCGIRIAG
                     QARDRLGKMMAVGVVALLFSHVFINIGMNIRIMPVTGVPLPLLSYGGSSVLGSLIAMG
                     ILQNVYIHRKTY"
     gene            complement(3427..>4620)
                     /locus_tag="VIL39_04105"
     CDS             complement(3427..>4620)
                     /locus_tag="VIL39_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5480638.1"
                     /translation="VWSPAEPGLNVVMTIDLRLQQAAERALQGLQKVLSGTIRGAAVV
                     MDVHSGDILAMVSLPSFDPNMYVRGITHEEWQRIVDLNAEKSRATFENYMPGSIFKTV
                     VGMAALEAGWNPEEIIHIEPNPAQPNKGHIRVGNHTFKDTAPPGDFAFRRALKLSSNS
                     YFITCGLRIGPEAIIRMGQRLHFGETAGLTNRQDAPGKFPSLQRLGTSGWNNVTTGNM
                     CIGQDPVWVTPLQITVLTAAIANGGEVLWPRLVDRIEAADPTFAAPPTVFPRGRVRDH
                     LGISARTVKIMHEAMLADTEDSDGTGRKACVPGLRICGKTGTAQVQDVHNTKIGQTTW
                     FASFAPYESPRYAVVVMVENGSSGGDTAPVAHDIYAALLDQERAAPGKPQTLAKNHSM
                     ATRAQ"
BASE COUNT          838 a         1455 c         1372 g          955 t
ORIGIN      
        1 gcgttctacc ctgtcgctgc atccgaccga ctgcggtctg cctgcgccga cggtgttttc
       61 ccggtgttac cccgaaggaa cccccgtgcg taccagatgt ggatagcgaa atcccctgtg
      121 ttttccagcc cttgttggct ctttcccgtc tttaaaagca acggagcgcg gttcttgcga
      181 gccgcgcccc gtctgcttat ctatcaactc tcaaaactat ccactatcaa ctaccttcag
      241 cccccgacgc tggccagctc ttcattggtc gcgacgttca cgatcttgaa ctgctcggcg
      301 tggagcgccg ggtccgcgcg gaaggagagc ttgccaaagt agcgcttctc catctcgatc
      361 agatgcttct cgtcctccgt gcggaggcgt tcaagaatgg tgggatgcac cacaatgcgc
      421 aactggaagt ccgactcgtc ccgcgagcgc tttttgagta tctcgctcag cttgcgctga
      481 atttcgacgc tcatggtcag cgcgctcttg accttgcccc ggcctttgca atacgggcag
      541 tcgtcataca cggctgcgtc cacgctttcg gagtggcgct ggcgcgtcat ctcgagcaac
      601 ccaagctggg agatgggcag gacgtgcgtc ttggctttgt cacggcgcaa gccgtccttc
      661 atccgctgat agacctgctg ttggtcgcgg cgcgctttca tgtcaatgaa gtcgagcacg
      721 attaaaccgc ccatgttgcg gaggcgcagt tgacgggcga tttcgtccgc cgcctccaga
      781 ttcaccttga gaatggttgc ttcctgatcc ttgccgccct tgtgccggcc cgtgttcaca
      841 tcaatggcca ccagggcttc ggtttcgtcc atgacgatat agccgccgct cttcaagtgg
      901 acctggcggg agaaggcatt ctccaattgc cgtgtgatgt tgaaacggtc gaagatcggc
      961 tgcggttcgg catagagctt caccttgtcg gcggaacgcc tggagatctt ggaaatcatg
     1021 tcccgcattc ggtcgtaggc tttctggctg tccacgacga tccgctccac gtcttcggtc
     1081 aggaagtcgc gcacggtccg ttcgatcaag tctggctcct ggaaaacgca agtggccagc
     1141 gcctgagact tgatgcgctc ctggatttgg gtccactcgt cgagcaggag ggccaggtcg
     1201 cgcacgaagt aacgcttctg ctgcccctcg cccacggtgc gcatgatgac ccccatgcca
     1261 tcgggaatag ccaattcgcg taagattttc ttaaggcgct ggcgctccgg ctggttctcg
     1321 atcttgcgcg agatgccgct ctggtctgaa ttcgggagca gcaccaggta acggcccggc
     1381 aggaccacgt tggtcgtgac ccgcgggcct ttggtgccga tgggaccttt ggtcacctgc
     1441 gcaatgatct cgctgcccgg cggatagagg cgcggaatgt ccttctgcgt gatcttgggc
     1501 cggtcgcgcc ggcgtccgcc ccgatccaca atctccaccc cgctgtcgaa gctgttcggc
     1561 acaatgtccc aataatgtag aaacgcgttc ttctcgaatc cgatatccac gaaggcggct
     1621 ttgaggccgt cttctagatt gcgcacccgg cctttgaaga tgctgccgac gagccgttct
     1681 tcggtcgtcc gctcgatggt gaattcctca agccggcctt cctcggacac ggccacgcgg
     1741 gtttcgagcg tttcggcgtt gatgatgact tccttgtgga ttctcttcac cggcttgatc
     1801 agccgctgaa ccttcttgac cagcgagctc gccgcgttcc ggatggtttc gactattccc
     1861 cgcgattgtt ccttgatggg cactggcgca aagggctttt gttcctgcag ttgcaccggg
     1921 gtcgccacgt ttggctcggg gaagttgtct ttacctgagc ccggcgcctc cgccgcgtct
     1981 ttgggcaaac cctcgggtgg caggcctgcg gcgatattct cggcgcggtc gatctcgttc
     2041 gcgtggcggt gctcatagac cttctccggc gcgggagggc cgcccaccgc gtccgccctg
     2101 gcttgggtgg cttcgcggtc ggggttttgc tgtccatgtc ccagcccgcc ggttgggcgg
     2161 aaacgcatgc cgcgccgcct tcgggaaaag tttcgatcac tcatatttaa taggtctttc
     2221 gatgtatata cacgttttgc aagatgccca tggcgattaa cgagccgagc actgatgacc
     2281 cgccgtaact tagcagcggc agtggcacgc ccgtcacggg cattatccga atgttcatgc
     2341 cgatatttat gaagacgtgg ctgaagagga gcgcgacgac gcccacggcc atcatcttcc
     2401 ccaggcgatc gcgcgcctgg ccggcgattc tgatcccaca gaacagaacc accccataca
     2461 gcgtgagcac gatcacgctg ccgacgaatc cactctcctc ggcaatgacg gagaagatga
     2521 agtcgttgtg cgccactggc cgcggcaggt acccaagggc attctgcgtg ccctgccgcc
     2581 agcctttgcc cgtcagcccc cccgagccca cggaaatcaa cgcctgtcgc acattgagcg
     2641 agtcatcgga ctgctgcctg cgcgagcgtt ctcgctccat cttggacgca ccggaagaca
     2701 gaggatattc tttgccgaaa tacaccagca gccggcgccg ctggtagtct tccaatttga
     2761 tctgcaagct cggtggcgcg aacagcacgt ctgccacgaa caacgcgccc agaaggccga
     2821 cgccgccgag caggcgcgcc aggtaccgcc gagggatgcc ggccacgaac aagatggcca
     2881 acccggtcgg cagcagcacc agcgccgagc cgaggtcggg ttctttgagg atcaacacga
     2941 acggcaggag catcagaccc agcgatttcc agagcgtcgc cagctcgtgc aactccccga
     3001 ccggccgact caggaaatgg gcttgggcca ggatgaaggc cagcttggca aactccgagg
     3061 gttggccctg gaacgggccc aaatcaagcc agcggcgagc gccccaactg tgggtcttgc
     3121 caatgccggg aatcagcact gcgaccagca gcaagatcgt gatgccataa agcacgaacg
     3181 accagcgagc gatcgtgtgg taatccatga agcacagtcc cgccgccgcg ccaatcccga
     3241 gcgcatacca gatgatctgg cgaaaccagg tgtgattaca gaagggcgcg gaaacggccg
     3301 attcgttgac catcgtcgcg ctatagacga agagcgtccc cagcaccatc agcccgagca
     3361 gggcggctat ctggagccga tcaatgcggg actcgcggcg ttggaggtca acttcaaaca
     3421 tgtcgattat tgtgctcggg ttgccattga gtggttcttg gctagagtct gaggtttgcc
     3481 cggggctgcc cgttcctgat cgaggagggc ggcgtaaatg tcgtgcgcga cgggcgcagt
     3541 gtcaccgccc gaagagccgt tttcgaccat caccaccacc gcgtaacgcg ggctttcata
     3601 gggcgcaaag gaggcgaacc aggtcgtctg gccgatcttc gtgttgtgca cgtcctgcac
     3661 ctgggcggtg ccggtcttgc cgcaaatccg caggccgggc acgcaagctt tcctgcccgt
     3721 cccatccgaa tcttccgtgt cggccagcat cgcttcgtgc atgatcttca ccgtgcgcgc
     3781 gctgatgccc aggtgatccc gcacccggcc gcgcggaaag accgtcggcg gcgcggcgaa
     3841 cgttggatcg gccgcttcga tgcggtccac cagccggggc caaaggacct cgccgccgtt
     3901 ggcgatcgcc gcagtcaaca cggtgatctg caatggcgtc acccagaccg ggtcttggcc
     3961 gatgcacatg ttgccggtgg tcacgttgtt ccaacccgac gtgccgaggc gctgcaagct
     4021 cggaaacttg ccgggagcat cctggcggtt ggtcagccca gcagtctcgc cgaaatgcag
     4081 ccgttgaccc atgcgaataa tcgcctccgg accgatgcgc aggccgcacg tgatgaagta
     4141 ggaattgctc gacagcttca gcgcccggcg gaacgcgaag tcgcccggcg gagctgtgtc
     4201 cttgaaagtg tggttgccga cccgaatatg gcctttgttg ggctgggcgg gattgggctc
     4261 gatatgaatg atctcctccg gattccaccc agcttccagg gccgccatcc ccaccaccgt
     4321 cttgaaaatg gagcccggca tgtagttctc gaaggtggcg cgactcttct cggcgttgag
     4381 atcaacgatg cgctgccatt cctcgtgcgt gatgccgcgc acatacatgt tcgggtcgaa
     4441 cgacggtaac gagaccatcg ccaggatgtc ccccgaatgc acgtccatga caaccgccgc
     4501 cccgcggatc gtgccgctta aaactttttg cagcccctgc aatgcgcgct cggccgcctg
     4561 ctggaggcgc aggtcaatcg tcatcactac gttcagacca ggttcggccg gactccagac
//
LOCUS       DATCGS010000204         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_213565, whole genome shotgun sequence.
ACCESSION   DATCGS010000204 DATCGS010000000
VERSION     DATCGS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213565"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2640
                     /gene="metH"
                     /locus_tag="VIL39_04110"
     CDS             <1..>2640
                     /gene="metH"
                     /locus_tag="VIL39_04110"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY5480639.1"
                     /translation="PGRVCFVAGALGPTGKTASLSPDVHNPAFRAITFDQLVTAYTEQ
                     TRGLLDGGVDVLLLETVFDSLNSKAALFAIDKYFEETGRRVPVMVSFTITDLSGRTLS
                     GQTVEAYWNSISHNKLLSVGINCAFGPKEMRPFIEELSGVADIYVSAYPNAGLPNPML
                     PTGFPETPETLAPQLKEWAENGWLNIVGGCCGTTPAHIRALAEAVRGLPPRVPPKIEP
                     ATRLSGLEALTIRPEANFVNIGERTNVTGSPKFAKLVLAGNYEAALTVAKQQVENGAQ
                     IIDVNMDEAMLDSEQAMTTFLNLVASEPDIARVPVMVDSSKWSVIETGLKCLQGKGIV
                     NSISLKEGEEKFVRQAKLIRRYGAAVVVMAFDERGQADSFERKIEVCQRSYRILTQQA
                     GFPPQDIIFDPNVLTVATGIEEHNNYAADFIRTAKWIKENLPFARVSGGISNVSFSFR
                     GNNVVREAMHSAFLYHAIRAGLDMGIVNPGMLEVYEEIPKDLLELVEDVLLNRRPEAT
                     ERLVRFAEKIKQKDEVEVAEQEWRKGTVEQRLSHALVKGVVEYIDQDTEEARQKYGRP
                     LLVIEGPLMQGMSVVGDLFGSGRMFLPQVVKSARVMKKAVAYLFPYMEAEKEASGNHQ
                     PQGRILMATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPAEKILQTAREKGADVIG
                     LSGLITPSLDEMVHVAKEMERQGFSVPLLIGGATTSKAHTAVKIAPGYSRPVIHVLDA
                     SRAVGVVSSLLNPRLKPAFASNIRAEYDKLRAQHADQKAKPLLTIAEARKRRTPIDWK
                     ASDLDMPAFTGIRILATDHQSPSTHHESRITHHASPISLSDIIPFIDWSPFFHTWEMR
                     GRYPAILEEPEAKKLFDEAQELL"
BASE COUNT          541 a          870 c          783 g          446 t
ORIGIN      
        1 aaccgggccg ggtttgcttt gtcgccggcg ccctcgggcc aaccggcaag acggcctcgc
       61 tctcccccga cgtccacaat cccgctttcc gcgccatcac ctttgatcaa ctggtcacag
      121 cctataccga gcagacgcgc gggctgctgg acggcggcgt ggatgttctg ctgctggaaa
      181 cggtcttcga ctccctgaac tccaaggctg cgctcttcgc catagacaag tatttcgaag
      241 agactggccg ccgcgtgccc gtgatggttt ctttcaccat caccgacctc agcgggcgca
      301 ccctgtcggg ccagaccgtc gaggcatatt ggaactccat ctcccacaac aagctgctga
      361 gcgtcggcat caactgcgcg tttggcccca aggagatgcg gccattcatc gaggagttgt
      421 cgggcgtcgc ggacatctac gtcagcgcct atccgaatgc cggtttgccc aacccgatgc
      481 tgccgaccgg cttccccgag acgccggaaa cgctcgcgcc gcagctcaag gaatgggccg
      541 agaacggctg gctcaacatc gtcggcggtt gctgcggcac cacgcccgcg catatccgag
      601 ccctggccga agccgtccgc ggcttgccgc cgcgcgtgcc acccaagatc gagccggcca
      661 cccgcctgag tgggctagag gcgctgacca ttcgtcccga agccaacttc gtcaacatcg
      721 gcgagcgcac caacgtcacc ggctcgccca agttcgccaa gctggtcctc gccggcaact
      781 acgaagccgc gctgaccgtg gccaaacagc aagtcgagaa cggcgcgcag atcattgatg
      841 tgaacatgga cgaggccatg ctcgactcgg agcaggcgat gacgaccttc ctgaacctcg
      901 tcgcatcgga gccggacatc gcccgcgtgc cggtcatggt tgacagttca aaatggtcgg
      961 tcatcgaaac cggcctgaaa tgcctccagg gcaagggcat cgtgaactcg atcagcctca
     1021 aggaaggcga agagaagttc gtgcgccagg cgaagctgat ccgccgttac ggcgcggcgg
     1081 tggtcgtgat ggcgttcgac gagcgcggcc aggccgacag cttcgagcgc aagatcgagg
     1141 tctgccagcg ctcgtatcgc atcctcaccc agcaagccgg cttcccgccg caggacatca
     1201 tcttcgaccc gaatgtcctc accgtcgcca ccggcatcga ggaacacaac aactatgccg
     1261 cggacttcat ccgcacggcg aaatggatca aggaaaacct gcctttcgcc cgcgtcagcg
     1321 gcggcatcag taacgtctcc ttctcgttcc gcggcaacaa cgtcgtgcgc gaggcgatgc
     1381 actcggcgtt cctctatcac gccatccgcg cggggctgga catgggtatc gtaaaccccg
     1441 ggatgctgga ggtctatgag gagattccca aagacctgct cgaattggtg gaggacgtgc
     1501 tgctcaaccg ccggccggag gccaccgagc gcctcgttcg gtttgccgaa aagatcaagc
     1561 aaaaggacga agtcgaggtt gccgaacagg agtggcgcaa gggcacggtc gagcaacgac
     1621 tgagccacgc gctcgtcaaa ggcgtcgtcg agtacattga tcaagacacg gaggaggccc
     1681 ggcagaagta tggccggccg ctgctggtga tcgaggggcc actcatgcaa ggcatgagcg
     1741 tcgtcggcga cctcttcggc tccggccgga tgttcctgcc gcaggttgtc aagagcgcgc
     1801 gcgtgatgaa gaaagccgtc gcgtatctct tcccttacat ggaggcggag aaggaggctt
     1861 ccggcaacca ccaacctcag ggccgcatcc tcatggccac ggtcaagggc gacgtgcatg
     1921 atatcggcaa gaacattgtc ggcgtggtgc tcggctgtaa caattatgag gtcattgacc
     1981 tcggcgtcat ggtgcccgcg gagaagatcc tgcagaccgc ccgtgagaag ggcgccgatg
     2041 tcatcggtct gagcgggctg atcacgccgt cgctggacga gatggttcac gtcgccaaag
     2101 aaatggagcg ccagggcttc agcgttcccc tgctgatcgg tggcgcaacc accagcaagg
     2161 cgcacacagc cgtcaagatc gctcccggtt actcgcggcc agtcatccat gtgctggacg
     2221 cttcgcgcgc ggtgggcgtg gtgagcagcc tgctcaatcc ccgcctcaaa ccggcttttg
     2281 cttccaacat ccgcgccgaa tacgacaagc tccgcgcgca gcacgccgat cagaaggcca
     2341 agccgctgct aaccatcgcg gaagcccgca aacgccgcac ccccattgac tggaaagcga
     2401 gcgacctgga catgcccgcc ttcaccggca tccgcatcct cgcgacagat caccaatccc
     2461 cgagcacgca tcacgaatca cgaatcacgc atcacgcatc ccctatcagc ctgtccgaca
     2521 tcattccgtt tatagactgg tcaccgttct ttcacacctg ggagatgcgc gggcgatacc
     2581 cagccatcct ggaggagcca gaggccaaga agctcttcga cgaagcccag gagctgctga
//
LOCUS       DATCGS010000205         4406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_464604, whole genome shotgun sequence.
ACCESSION   DATCGS010000205 DATCGS010000000
VERSION     DATCGS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464604"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VIL39_04115"
     CDS             complement(<1..346)
                     /locus_tag="VIL39_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480640.1"
                     /translation="MGVFGLLLPFLFLASGSLNASTEISIQNSVCRVVFDPVTLAVTL
                     QSGSHPAILVSAAQTNLGNVAGLERSATGARWSLPERKVSVTLELEGSRLLAHVLAAE
                     PGEFTFPVIAETA"
     gene            complement(390..1328)
                     /locus_tag="VIL39_04120"
     CDS             complement(390..1328)
                     /locus_tag="VIL39_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480641.1"
                     /translation="MNGPRIRGWLVAAVMVLWAPLATRATNAVAPDAGGANQTAPTAQ
                     AAPAARYSSSVADIVKLVDAKVDAEVIKTYVRNSPTAYNPSATEIIALKDRGVGSEIL
                     TAMLQHGAEVRAQSVQAAQAAQSAPAPQVNPGASTAYAPAYAYSAQPVYQDYAYSYPV
                     SSYAYPYYDYGYPGYSYASYGYGYSWPYYWPSLSFGFGCYPYRGWGGYGYGYGGRGYY
                     GGHAYYGGRGYYGGHGYYGGAGYRGQGGRSAPYAGSSGGFRSYGGGARSASFASPAGG
                     YRGGGGFSGHQASFGSMGGARGGGGFGGGHSSGRGR"
     gene            complement(1505..2401)
                     /locus_tag="VIL39_04125"
     CDS             complement(1505..2401)
                     /locus_tag="VIL39_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480642.1"
                     /translation="MKINRTWLSTGQHSCPSRGFTLIELLVVIAIIAILAALLLPSLA
                     RAKLKAQGVQCMNNHRQLAIAWRMYTEDSADKLLYASATVSSLSTWDSTWPYTWCTGK
                     MDFNAGNASNWDPNVDIYKSPMWPYCGKSLGIWRCPADRSFLTVGGVRKPRIRTMAMN
                     AYLGGFGGQPIGTGNMTAYTLYLKYSDLVRPGADRIYVFVDEREDAINWGNAIFDMTG
                     YSPTNPNLYRFLDIPAGYHGNAGGFSFADGHSELKKWRDPRTMPPVSDGSVIFDGSTP
                     RSSPGNADIAWMQDKTTRPKQP"
     gene            2624..3577
                     /locus_tag="VIL39_04130"
     CDS             2624..3577
                     /locus_tag="VIL39_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5480643.1"
                     /translation="MNFLVTGGAGFIGSHVCERLLHSGHAVWAFDDLNAFYDPRIKQR
                     NLRDIQALARPFEFIRGDLTDRAAVDGLLGRVKFDQIIHLAARAGVRPSLEEPALYQR
                     VNVEGTVNVLEAARLNRVPKVIIASSSSVYGVNSKVPFAESDPIFRAISPYAASKLAC
                     EALGHVYHHVYGLDVVMLRFFTVYGSRQRPDLAIHKFARLISAGRPIPVFGDGTAARD
                     YTYITDILDGVMACTQREFGYEVFNLGESQTVRLDQLIALLEGALGKKAVIDRQPPQP
                     GDVPITCADISKARAKLGYAPRVKIEQGIPLFVDWFTRNIL"
     gene            3586..4155
                     /locus_tag="VIL39_04135"
     CDS             3586..4155
                     /locus_tag="VIL39_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480644.1"
                     /translation="METTRHYQRPPFEDALIAWKALLTERGLSTDIVWILEENLCFEK
                     DPGTPARVKLGFQTRFTAYPPDAAKLTYHHFAELDARLVFYRLGANAGRSICLLLCDA
                     WFEPKNETHGYVRRDDWLVSFFPGANEEIEEVTDAKRWQERVVRGRPLSAVDFCMTLA
                     ALRELKAHGRVLTPDERFGLKILRSMRGT"
BASE COUNT          860 a         1395 c         1315 g          836 t
ORIGIN      
        1 aagccgtctc ggcgataacc gggaaggtga actcgcccgg ttccgcggcc aggacatggg
       61 ccagcaaccg gctgccttcc aattcgaggg tgacggaaac ctttcgctcg ggcagcgacc
      121 accgggcgcc ggtggcgctt ctctccagtc cggccacgtt ccccaggttg gtttgggcgg
      181 cagaaaccag gatggccggg tggctgccgc tctgcaacgt cacggccagg gtgacgggat
      241 caaaaacgac gcggcagaca ctgttctgaa tggaaatctc cgtgcttgca ttcagacttc
      301 ccgaggccaa aaacaagaac ggcagcagca gcccaaatac acccattgcg taaccccacc
      361 ttgggcaaga catctgttaa gcgctaggct cagcgtcctc ggccggagga gtgcccgccg
      421 ccgaaacctc caccgccccg ggctccgccc atgctgccaa aggaggcttg gtgtccgctg
      481 aaccctccac caccccgata accaccagcg gggctcgcga aggaggctga ccgggcacca
      541 ccgccgtaag agcggaagcc gccagaagag cctgcataag gagcggatcg cccgccttga
      601 ccacgatagc cggccccgcc gtagtagccg tggccaccat agtagccccg gccgccgtag
      661 taagcatgac cgccataata gccgcgacca ccgtagccat agccgtagcc accccaaccg
      721 cgatacgggt agcaaccgaa gccaaaagac aaggagggcc agtaatacgg ccagctatag
      781 ccgtagccgt aggacgcata actgtacccg ggatagccgt aatcgtaata agggtacgcg
      841 taagaactca cgggatagct gtatgcgtaa tcctggtaaa cgggctgggc gctataggcg
      901 taagccggag cataagcggt ggacgcgccc gggttgacct gcggtgcagg ggcgctctgg
      961 gccgcctgcg cggcttgcac cgactgggcc cgcacctcgg cgccgtgctg caacatggcc
     1021 gtgaggatct ccgacccgac gccacgatcc ttcagcgcga tgatttccgt ggcactgggg
     1081 ttgtaggcgg tcggtgagtt cctgacatag gtcttgatga cctccgcatc cactttcgcg
     1141 tccaccagct taacgatgtc cgcgacgctg gaagaatatc ttgctgccgg agcggcctgg
     1201 gctgttggag cggtctgatt agcgccgcca gcgtccgggg cgacggcatt ggttgctctt
     1261 gttgccaggg gcgcccacag caccattacg gccgccacca gccaccccct gattcgaggg
     1321 ccattcacag ctacgattcg cttcgctatt ttcataacca ctgccagcat tggcggttac
     1381 cattactatc ccccctcagg ccccagtgtc aagggccggc aggacagccg gagagccgca
     1441 gcccggaaat tcaaaggctg aaacccgaac ggtaaagcaa gcgaaaggat ttcccggcag
     1501 cgtcctacgg ttgtttcggg cgcgtcgtct tatcctgcat ccaggcgatg tccgcgttgc
     1561 cgggggagct gcgtggggtg gagccgtcaa agattactga gccgtcgctg accggaggca
     1621 tcgtgcgggg gtctcgccac ttcttgagtt cagaatggcc atcggcgaac gagaatccgc
     1681 cggcgttgcc atgatagccg gcggggatat ccaggaaccg gtaaaggttc ggattggtag
     1741 gcgagtagcc agtcatgtcg aagatggcat tgccccagtt gatggcatcc tcgcgttcgt
     1801 ccacaaacac atagatcctg tccgctccgg gtctgaccag gtcggaatac ttcaagtaca
     1861 gcgtataggc ggtcatgttt cccgtgccga tcggctggcc cccgaagccc cccaggtaag
     1921 cattcatggc catggtgcga atccggggct tccttacccc cccgaccgtt agaaacgagc
     1981 ggtccgccgg gcaccgccag atccccaggc ttttgccgca atatggccac atgggactct
     2041 tgtagatgtc cacgttcgga tcccaattgg aggcattgcc ggcattgaaa tccatcttcc
     2101 cagtgcacca ggtgtacggc caagtggaat cccaagtgct gagcgatgag acggtggcac
     2161 tggcgtagag caacttatcg gcactgtcct cggtgtacat gcgccacgcg atggcaagct
     2221 gacggtggtt gttcatgcac tggacgccct gggccttgag cttggcgcgg gccagggacg
     2281 gcagcagcag cgctgcgagg atcgcgatga tggcaatgac gaccagcagt tcgatgagag
     2341 tgaaaccgcg agaggggcac gaatgctgcc cggtggataa ccaggttcta tttattttca
     2401 tgcgagcgtg gtttgttgcc aacgatcaac gctcttttgc catgccgccc cgccaattgc
     2461 aagcataaaa tgcggacgca cgacgaaacg tgttgcctca cttatgcaca catcggcact
     2521 aatgccagtc ctgacgccgg cccggcactc gaccggacag gacgcgctgg ccgccattgt
     2581 cgagggtctc gacatgacta gctttcccag gtaatctgcg ggtgtgaact tcctcgtcac
     2641 gggtggcgcc ggcttcattg gctcgcatgt ctgcgagcgc ctgctccatt ccggccatgc
     2701 cgtctgggcg ttcgatgatc ttaatgcctt ctacgacccg cgcattaagc aacgcaacct
     2761 gcgcgacatt caagcccttg cccggccctt tgaattcatc cgcggcgatt tgaccgaccg
     2821 cgcggcagtg gacgggctgc tcggccgcgt taagttcgac cagatcatcc acctcgccgc
     2881 gcgtgcgggg gtgcggccca gcctcgagga gccggcgctc taccagcgcg ttaatgtcga
     2941 aggcacagtc aacgtgctcg aggccgcgcg gctcaaccgc gttccgaagg tcattattgc
     3001 ctcctcctct tcggtgtatg gcgttaattc gaaggtgccg tttgccgaga gcgaccctat
     3061 tttccgcgcc atctcgccct acgccgcgag caagctggct tgcgaggcgc tggggcatgt
     3121 ttatcaccac gtctatggcc tcgacgtggt catgctgcgc tttttcaccg tgtatggctc
     3181 gcgccagcgt ccggacctgg ccatccacaa attcgcccgg ctcatcagcg ccggccggcc
     3241 catccccgtt ttcggcgacg gcacagcagc gcgcgattac acttatatca ccgacatcct
     3301 cgacggcgtc atggcctgca cgcagcggga gttcggctat gaagtgttca atttggggga
     3361 atcgcaaacc gtgagacttg accaactcat cgctttgctc gaaggcgcgc tgggcaagaa
     3421 ggccgtgatt gaccgccagc cgccgcagcc gggcgatgtg cccatcacct gcgcggacat
     3481 ctcgaaagcg cgcgccaaac tcggctacgc cccgcgcgtg aaaatcgagc agggcattcc
     3541 gctttttgtt gactggttca cccggaacat cctctaatgg aagcaatgga aactactcgc
     3601 cattatcagc gcccaccttt cgaagacgct ctaattgcgt ggaaagccct cctgaccgaa
     3661 cgcggccttt cgaccgacat cgtatggatt ctggaggaga acctctgttt cgagaaagac
     3721 ccggggacgc ccgcccgcgt caagctcggc ttccagaccc ggttcaccgc ctatccgccc
     3781 gatgccgcca aactcaccta ccatcatttc gccgaactgg acgcccggct ggtcttttac
     3841 cgcctcggcg ccaacgccgg acgctccatc tgcctcctgc tctgcgacgc atggttcgag
     3901 cccaagaatg agacccacgg ttacgtgcgc cgcgacgact ggctggtttc tttcttcccc
     3961 ggtgccaatg aggaaatcga agaggttacc gacgccaaac gctggcaaga gcgcgtagtg
     4021 cggggccgtc ccctgagcgc ggtggacttc tgcatgacgt tggcggccct tcgcgagctc
     4081 aaagcccatg gccgcgtgct gacccccgac gaacggtttg gcctgaagat cctgcgctcg
     4141 atgcgcggga cctgacccgt tcgccgcccc gcctggttta ggagtgcggt tgtcgaaact
     4201 gagatttgag actcgacgta ggcgccgcgg agcgcaagcg cgccttggat cgcgcattcg
     4261 gctgcctctc ggcatcggaa tcggaaacca tggcgagcgc catcgaaact cattgcgaaa
     4321 gggttgatgc cggccagtgg tgacgtcgtt cagaacttcc ttaaggccgt ggtcgtgctt
     4381 tccccggggg tgacaacggc ggaaca
//
LOCUS       DATCGS010000206         7149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_68313, whole genome shotgun sequence.
ACCESSION   DATCGS010000206 DATCGS010000000
VERSION     DATCGS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68313"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..619)
                     /locus_tag="VIL39_04140"
     CDS             complement(161..619)
                     /locus_tag="VIL39_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY5480645.1"
                     /translation="MPRIRLLLRYWLPLLVWMLVIFTASGDRMSFDHSSRIIGPFLHW
                     LLPHLSDAAVHAVIVIVRKGAHVTEYAVLALLLWHALRKPPEANVPSWRWSQAGLALA
                     LAALYAAGDEIHQTFVPSRQGSVWDVLLDTTGAAIGLLCLWAIGRLRKRW"
     gene            717..1457
                     /locus_tag="VIL39_04145"
     CDS             717..1457
                     /locus_tag="VIL39_04145"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY5480646.1"
                     /translation="MLKLGVNIDHVATLREARYRGRGHGEPDPVAAALDCEAAGAHGI
                     TAHLREDRRHIQDRDVWKLREVIKTRLNLEMASVPEIIAIALKLKPEIVCLVPERRQE
                     VTTEGGLDVAGNVQSLTETRKRMNDGGIEVSLFITPDAAQIEAAARTGSQFIELHTGA
                     FDEAFDDESRCKAELERLIAGAKQAHALGLKVNAGHGLNYRNLPLLHRVPHLVELNIG
                     HTIISRAVTVGVKAAVQEMLRLMANYRG"
     gene            1468..1851
                     /gene="acpS"
                     /locus_tag="VIL39_04150"
     CDS             1468..1851
                     /gene="acpS"
                     /locus_tag="VIL39_04150"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY5480647.1"
                     /translation="MILGIGIDIIEVARIQASHERFGERFLNRILLPSEISYCLSYKA
                     PGPFLAVRFAAKEAISKAFGTGIGAQLGWQDMEVGRKESGEPFVILHEGGRKLLEARG
                     ARVVLISLSHTQNYATAVAVLESKD"
     gene            1958..2578
                     /locus_tag="VIL39_04155"
     CDS             1958..2578
                     /locus_tag="VIL39_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY5480648.1"
                     /translation="MQPEPVSAAVPMSLIYPLPSIVERIDLAHLFPTPQSLEVELGSG
                     DSSFLVEYARQYAGHNFIGVERLLGRIRKLDRKGRRAGLTNLRGMRIESAYFLEYLLP
                     PHSAVSLHVYFPDPWPKRKHRRNRLINERFPALAQQALAPGGTVYLRTDDQDYSEQML
                     AVFAAAPAFRPVETPPELSALLTDFEKDFHARGIKTLRAAYRIGDR"
     gene            complement(2629..3060)
                     /locus_tag="VIL39_04160"
     CDS             complement(2629..3060)
                     /locus_tag="VIL39_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY5480649.1"
                     /translation="MKRFLQSWMINTLAVLAAVYIVPGIRFRDDSLWTPFVTSLVLGI
                     LNAFIRPILMFLALPLLIFTLGLFTLVINALLLFFVSLLLGRYFVVDNFGAAFLGALV
                     ISIVSLVLNFITGTNRARVRVERRRRPPDSDRGGNGPVIDV"
     gene            complement(3057..4025)
                     /locus_tag="VIL39_04165"
     CDS             complement(3057..4025)
                     /locus_tag="VIL39_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEY5480650.1"
                     /translation="MLYPLTFQPIFKQRVWGGRELERLYHKPLPPGVPIGESWEITDR
                     PGDVSVIANGPLAGKDLRWLMENHVAGLLGDAKSHRGRFPLLIKILDAQEKLSLQVHP
                     PAARATELGGEPKTEMWYIAEAAPGAELYVGLKHGVTRAEFEGKVQTGTVAECFHRVS
                     VQPGDAMFLPSGRVHALGAGLVVFEIQQNSDTTYRVSDWNRLGLDGKPRELHIPQSLA
                     SINFNDFEPSLVPRGFALEGAQQVRPLVRDPLFTVEAHHAGAGANVPLRPGKMQIIAV
                     LTGQMQVGSGQATVALVPGQFCLVPASLASSALRTETPTTFLRVEL"
     gene            complement(4087..5085)
                     /locus_tag="VIL39_04170"
     CDS             complement(4087..5085)
                     /locus_tag="VIL39_04170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY5480651.1"
                     /translation="MPMKHQLDFEKPIVELQNKLEELKKHPETHSLDISFEEEAGLIQ
                     KKLEETKRQIFANLSAWDRIKIARHPKRPFTLDYLQTAFTGFSELHGDRLFAEDRAVV
                     GGFACLGAHKVMVIGTQKGRDTKENIRRNFGSAHPEGYRKALRLMRLADKFRLPIITL
                     IDTAGAYPGIGAEERHIAEAIAVNLREMMILQVPIIAVVIGEGGSGGALGIGVADRVL
                     ILENAYYSVISPEGCAAILWKDRSAAAKAAEALKITARHLLELGLVDEIVPEPLGGAH
                     TDREMAAQTLRDHLLKHLEQLKTLSPADRLKQRYAKFRAFGHFTEKQPAVAEKIGG"
     gene            complement(5417..7033)
                     /gene="zwf"
                     /locus_tag="VIL39_04175"
     CDS             complement(5417..7033)
                     /gene="zwf"
                     /locus_tag="VIL39_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5480652.1"
                     /translation="MKSKIRIFEKVRPIGDGLGVNQSAQFDDLLTCRLSEGHKAVEPC
                     SIIIFGASGDLTARKLIPALYHLCGEKQMPPAFRVIGLARREKTSASWRNELRSALDE
                     FSRTKPVDDQIWQDFSQHVFYCQGDLTAAATYQALEKQLAAFGSEPLRRNLLFYLATS
                     PSQFSEVVEQVHGSGLLRKEEGEGWQRIVVEKPFGHDLESARQLNRELTRYASEQQVF
                     RIDHYLGKETVQNIMMFRFSNAVFERLWNRDAIDHFQITVSEKLGVDQRGGYYEEAGA
                     LRDMVQNHLLQVLSLVAMEPPVSLEAEAIRDEKVKLLKSIRPLSPEDVARQVVRGQYF
                     AGTVDGESRPGYRQEPKVKPDSNVETYVALKLFIDNWRWSGVPFFLRTGKNLPLSTSE
                     VRIQFRPTPQVLFAAQCGQKLDPNSLTLRLQPNEGISLRVNGKVPGTSVAVRPVRMHF
                     SYDAEFGAYTPEAYERLLLEAMAGDATLFIRRDEVETAWQIVDGIRQAWQGKSLSNRE
                     FYAAGTWGPVAADDLLAQSGHAWREPLVIK"
BASE COUNT         1414 a         2190 c         2211 g         1334 t
ORIGIN      
        1 cgggccaggg ctgcatcaag ctcccgattc cgaccaagtg tggtagggcg tttcgccgaa
       61 acgtccgtgt ttgcgcgagt gcggacgcct cggcgaggcg tccctaccaa acaagcgcgc
      121 ctaaacgcga gcttgaggca gccccgcgtc gcccggtgcg ctaccagcgt tttcgcagtc
      181 gcccaatcgc ccacaggcaa agcaagccaa tggccgcacc ggtggtgtcg agcaagacgt
      241 cccacacact gccctggcgc gaagggacga aggtttggtg gatttcatcg cccgccgcgt
      301 agagcgccgc cagggccagg gccaggccgg cttgcgacca acgccaggaa gggacattcg
      361 cttcgggcgg tttgcgcagc gcgtgccaga gcaagagggc caataccgca tattcggtta
      421 catgggcgcc cttgcggacg atgacgatga cggcatggac cgccgcgtca gacagatgcg
      481 gaaggagcca gtgcaggaac ggtccgatga tgcgtgagga gtgatcaaag gacatccggt
      541 cgccggacgc ggtgaagatg accaacatcc agaccagcag cggcaaccaa tacctgagga
      601 gcaatcgtat tctcggcacg acacatggaa acagaaaact gccagccgcg gcaatcctgt
      661 agagaactaa tgtttgggtt gcggacagcc tgacgggtga accataattg aagcgtatgt
      721 taaagctggg cgtaaatatt gatcacgtag cgacgctgcg ggaagcgcgc tatcgcggac
      781 gcgggcacgg cgagccggac cccgtagccg ccgcgctcga ctgtgaggcg gcgggcgcgc
      841 atggcatcac cgcccacctg cgcgaggacc gccgccatat ccaggatcgg gatgtctgga
      901 agctgcgcga ggtcatcaag acccggctca acctggaaat ggccagtgtg ccggagatca
      961 ttgccatcgc gctgaagctc aagccggaga tcgtttgcct ggtacccgaa cgacggcaag
     1021 aggtcaccac cgagggcggc ctggatgtcg ccgggaatgt ccaatcgtta accgagacgc
     1081 gcaaaaggat gaacgacggc gggattgagg tcagcttgtt catcacgccc gatgcggcgc
     1141 agattgaagc cgccgcccgc acgggcagcc agttcattga gctgcacacc ggcgctttcg
     1201 atgaggcgtt cgacgacgag tcgcgatgca aggcggagct ggaacgattg atcgccggcg
     1261 cgaagcaggc gcatgcgctc ggcctgaagg tcaacgccgg gcatggcttg aattaccgga
     1321 acctcccgct gctgcatcgc gtgccgcacc tggtcgagct gaatatcggc cacaccatca
     1381 tcagccgcgc cgtcacggtg ggggtcaagg ccgcggtgca agagatgttg cggttgatgg
     1441 cgaactaccg ggggtgaaac tatcgaaatg atcctgggca ttggcatcga cattatcgag
     1501 gtggctcgca ttcaggcttc gcatgaaagg ttcggtgagc ggttcctgaa ccgcattctg
     1561 cttccgagcg agatcagcta ctgcctgtcg tacaaggcgc cgggaccgtt cctggcggtg
     1621 cgctttgcgg cgaaggaggc tatctccaag gctttcggca ccggcattgg cgcacagctc
     1681 ggctggcagg atatggaggt cggacgcaaa gagagcggcg agccattcgt cattctgcat
     1741 gaaggcggcc gcaagctcct ggaggcccgc ggtgcccggg tggtgttgat cagcctgagc
     1801 cacacccaga attacgccac cgcagtggca gtcctggaaa gcaaagactg aatccgtcag
     1861 gggcagtgcc cttggcgcgg accccatgct caccggccat gaatctcgca ggacgctatg
     1921 ggttgccgcc gcctcgctcg gcctgctaac ctcggacatg caaccagagc cggtttcagc
     1981 cgcagtgccg atgagcctga tctatccgct gccctcgatt gtcgagcgca ttgacctggc
     2041 acaccttttt ccaacgccgc aatcgctcga ggtggagctg ggcagcgggg acagctcgtt
     2101 cctggtggag tatgcccggc aatacgcagg acacaatttc atcggggtgg aacggttgct
     2161 gggacgcatc cgcaaactgg accgcaaagg ccggcgggct gggctgacga acttgcgcgg
     2221 tatgcgcatc gagtcggcct acttcctcga atacctgttg ccgccccact cggcggtgtc
     2281 cctgcacgtt tactttcccg acccgtggcc gaagcggaag caccggcgga accggctgat
     2341 caatgaacgt tttcccgcgc tggcccagca agcgcttgcg ccgggcggca cggtttatct
     2401 gcgcacggat gaccaggatt attccgagca gatgctggcc gtgtttgccg ccgcccctgc
     2461 gttccgcccg gtggaaacgc cccccgagct cagcgccttg ctgactgatt ttgagaagga
     2521 tttccatgcg cgcggaatca agaccctccg ggcggcctat cggattggcg accggtgatt
     2581 gtgctactga tcactgatca ccgactactg atcaccagcc accccctctc aaacgtcaat
     2641 gacagggccg ttgccgccgc ggtcggagtc gggtgggcgg cgccggcgct cgacgcggac
     2701 gcgggctctg ttggtgccag tgatgaagtt caggaccagg gaaacgatac tgatgaccag
     2761 cgcgcccaga aaggccgctc cgaagttatc caccacaaag taacgcccga gcagtaaact
     2821 tacgaagaac aacagcagcg cgttgatgac cagggtgaag agccccaggg taaaaatgag
     2881 caaaggcaac gccagaaaca tgaggatggg ccgaatgaag gcattgagga tgcccagaac
     2941 cagggaggtc acgaaggggg tccagaggct atcatccctg aagcggatgc ccgggacgat
     3001 atagaccgct gccaacacgg ccagcgtgtt gatcatccag ctctggagaa acctcttcac
     3061 agttcgacgc gcaggaaagt ggtcggggtc tctgtgcgca acgccgacga cgccaggctc
     3121 gcgggcacga ggcagaattg gcccggcacc agggcaacgg tcgcctggcc gctgccaacc
     3181 tgcatctggc cggtcaacac ggcgatgatc tgcatcttcc cgggccggag cggcacgttc
     3241 gcgccagcgc ccgcatggtg ggcctcgacg gtgaagagcg gatcgcgcac gagcggtcgc
     3301 acctgttgcg ccccttccag ggcgaagccc cggggcacca gcgatggctc gaagtcgttg
     3361 aagttgatgc tggccaggga ctggggaatg tggagttcgc ggggtttgcc gtccaggccc
     3421 agacggttcc agtcggagac gcggtaggtg gtgtccgagt tctgctggat ttcaaagacg
     3481 accagtcctg cgccgagcgc gtgaacgcgg ccgctgggca ggaacatggc gtctccaggc
     3541 tgcacgctca cccgatgaaa gcactcggcg accgtgccgg tctggacctt gccttcgaac
     3601 tcggcgcggg tgacgccgtg tttcaggccg acatacagct ccgctcccgg ggcggcttcc
     3661 gcgatgtacc acatctcggt cttcggctcg ccgccgagtt cggtcgccct ggcagcgggt
     3721 ggatgcacct gaagcgagag cttttcctga gcgtcgagga tcttaatgag caaagggaaa
     3781 cggccgcggt gcgatttggc atcgccaagc aggccggcga catgattctc catgagccag
     3841 cgcaggtcct tcccggcgag cgggccgttc gcaatgacgc tgacatcgcc cgggcggtca
     3901 gtaatctccc acgactcacc aatcggcacg ccgggcggca ggggtttgtg gtaaaggcgc
     3961 tcaagctcgc gtccgcccca gacgcgctgc ttgaagatgg gctggaaggt caaagggtag
     4021 agcataggga aggattttca gctaaaggca gaatgctgca gacaccgggc cttggagcca
     4081 tgaggctcat cccccgatct tctccgccac cgctggctgc ttctcggtaa aatgcccaaa
     4141 ggcgcggaac ttggcgtagc gctgcttcaa gcggtcagcc ggagacaggg tcttgagctg
     4201 ctctaggtgt ttgagcagat gatcgcgcag cgtctgagcc gccatctctc gatccgtatg
     4261 ggcgccgccg agcggttcgg ggacaatctc gtccaccagc cccaactcca gcaggtgccg
     4321 ggcggtaatc ttcagcgctt cagcggcctt ggcggcggcg gaacgatcct tccagagaat
     4381 cgccgcgcag ccttccgggc tgatcaccga atagtaggcg ttttccagaa tcagcacccg
     4441 gtcggccaca ccgatgccca gggcgccgcc cgagccacct tccccgatca ccacggcgat
     4501 aatgggcact tgcagaatca tcatctcgcg caggttcacc gcgatggcct ccgcgatgtg
     4561 gcgctcctcc gccccgatgc ccggataggc tccggccgtg tcaatgagag tgatgatcgg
     4621 cagccgaaac ttatccgcca accgcatgag ccgcagggct ttgcggtagc cttccggatg
     4681 tgcggagccg aaattgcgcc ggatgttttc cttggtgtcc cggccttttt gggtgccaat
     4741 caccatcact ttgtgcgcac cgaggcaggc gaacccgccg accaccgccc gatcctccgc
     4801 gaacagccgg tcgccgtgca gctcgctaaa gccggtaaaa gccgtttgga ggtagtcgag
     4861 cgtgaacggc cgtttcggat gccgggcaat cttgatgcgg tcccaggcac tgaggttggc
     4921 gaagatctgg cgtttggttt cttccagctt cttctggatt aaaccggctt cttcctcgaa
     4981 actgatgtcg agggagtgcg tctccggatg ctttttcaac tcctcaagct tattctgtag
     5041 ctccacaatc ggcttttcga agtcgagttg gtgtttcatc ggcataagcc tagccgcatt
     5101 tcgcctgcca ggcaacgccg atttattaca ggcaaagagc cggatttgca tccggctctt
     5161 agtggtgacg aggtttacac gccttcaccc aggcgctcag aagggtaatg cggccaacta
     5221 ttcgatcgct gttcgaaccg cattacaagc aaaacctctc tgctaaccct aaacgaacag
     5281 cgaaatcttc tgtcgctctg agtctatgat acaccagccc ggcaatctgt tcaaactctt
     5341 tttttgccat ctttccagct ccctgccctt ctcgcggaca gaggacttac ccggcctaat
     5401 ctggcaccgc cgctgcttac ttgatgacca gaggctcgcg ccaggcgtgg ccgctctggg
     5461 ccaacagatc gtcggccgcc accggccccc aggtgcccgc ggcgtaaaac tcgcggttgg
     5521 acagcgactt gccctgccag gcttggcgaa tcccgtccac gatttgccac gcggtctcga
     5581 cctcgtcgcg gcggataaaa agcgtggcgt cgccggccat cgcttcgagc agcagccgtt
     5641 cgtaggcctc gggcgtatag gcgccgaact cagcatcgta actgaaatgc atgcgcaccg
     5701 gccgcaccgc cacgcttgtc ccgggcacct tgccgttaac ccggagggag atgccttcgt
     5761 tgggctgcag tcgcaaagtc agcgagttcg gatccagctt ctggccgcac tgggccgcga
     5821 agagcacttg cggcgtgggc cggaactgaa ttcgcacctc actggtgctc agcggcagat
     5881 tcttgcccgt gcgcaggaaa aacggcacgc ccgaccagcg ccagttgtca atgaagagct
     5941 tcagcgccac gtaagtctcg acattggagt ctggcttgac cttcggctcc tggcggtagc
     6001 ccggccgcga ttcgccatcc accgtgcctg caaaatactg cccgcgcacc acttgccggg
     6061 cgacgtcctc cgggctcaat ggccggatcg acttgagcag tttgaccttt tcgtctcgga
     6121 tcgcctcggc ttcgagggag acgggcggtt ccatcgcgac cagcgacagg acttgcagca
     6181 gatgattctg caccatgtcg cggagagcgc cggcctcctc gtagtagccg ccgcgctggt
     6241 ccacgccgag cttctcgctg acggtgatct gaaagtgatc gatggcatcg cggttccaga
     6301 gccgctcgaa aacggcgttc gagaagcgga acatcataat attctgcacc gtctctttgc
     6361 cgaggtaatg gtctatgcgg aagacctgct gctcgctggc gtagcgggtc agctcccggt
     6421 tcaactggcg ggccgattcc agatcgtggc cgaagggctt ttcgaccacg atccgttgcc
     6481 agccctctcc ctcttccttt cgcagcagtc cggagccatg cacttgttcg accacctcgc
     6541 taaactggct gggcgaggtg gcgagataga agagcaggtt ccgccgcagc ggttcgctgc
     6601 cgaaagcggc aagctgtttc tccagcgcct ggtaggttgc cgcggcggtg agatcgccct
     6661 ggcaatagaa cacatgctgc gagaaatcct gccagatctg atcgtccacc ggtttggtgc
     6721 gcgaaaactc gtccagcgcc gaccgcagct cattccgcca ggaagcagag gtcttctcgc
     6781 gccgcgccaa gccgataacg cgaaaggccg ggggcatctg cttctccccg caaaggtggt
     6841 acaaggcggg tatgagcttg cgcgccgtca gatcgccgct cgcaccgaag atgataatcg
     6901 agcatggttc caccgccttg tgcccctcac tcaggcgaca cgtcagcagg tcatcaaatt
     6961 gcgcggattg gttcacgccc aaaccatcgc cgatgggccg caccttttca aatattctta
     7021 ttttagactt catgaattat gattatccac ttaatgcagg ttgatggttt tcgtagatat
     7081 tgcaaatgcc aagaccgaca ttcccgaaag gcctgcgtga attccggcaa aagttttcga
     7141 gtgacgcga
//
LOCUS       DATCGS010000207         4437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_167919, whole genome shotgun sequence.
ACCESSION   DATCGS010000207 DATCGS010000000
VERSION     DATCGS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167919"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="dctP"
                     /locus_tag="VIL39_04180"
     CDS             <1..633
                     /gene="dctP"
                     /locus_tag="VIL39_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5480653.1"
                     /translation="LAKGYVVLFWSDSGWVRFFSKSPVLHPDDLRKLKVFSGSDSAAA
                     YDLWKALGFSPVSLDATGIPQGLLSGTISAVPTVPIYALAGQLDSQAKYMLELNWGPL
                     VGAAVVHKKTWDRVPAAAREAMLKIAAETGRQVRAAGRAESDAAVAAMVKRGLVVQKV
                     TPEVDQEWRAMIDKVQGQIRGKVVPADLFDEAQRFAKDYRAAGGKGVEKK"
     gene            932..2809
                     /locus_tag="VIL39_04185"
     CDS             932..2809
                     /locus_tag="VIL39_04185"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5480654.1"
                     /translation="MSSGAVLPPDLLGSPESTTLSRRRRLFCQGENLLLVIPLAAMLL
                     LPVVEIILRLVFKTGISGSSAIVQHLTLIVGMLGGAIAARDQRLLALSPVQTLLKGRA
                     RTAAQIFSSGFAAAITFFLFLASLQYVLDMRPLGKTLVYGIPVWVIQLILPIGFGLIL
                     LRLLWHASEKYGGRALALALAAAAVGMAIWAPVSPERLMIPALVALGLATLLGAPVFT
                     ALGGAALILFWGHDLPIQAVPLKHYSLTTNNMLPSIPIFTLAGYFLAEGGASKRLVRV
                     FQALVGQFRGGPAIVTALVCAFFTSFTGASGVTILALGGVLMPVLLAARYSERNALGL
                     LTGAGSLGMLFPPCLPLILYTIVANHSAHADLTIKEMFLGGIGPGILLVALTAWWGIR
                     KGPKEALDRPVFQWSEAWTALWDAKWEMLIPVVAIVSLFYVPTTVEAAAVTATYALLV
                     TTVIHRDLRPIKDLPRVITECGLLVGGVLLILGVALGFTHYLVDAQLPDKMVEWATQM
                     VGSKWLFLLGLNVVLLLVGGLVEIYAAIIVVVPLLVPLGVAFGLDPIHLGIIFLANME
                     LGFLCPPVGLNLLLSSYRFNKPMAEVTRATFPMLLVLLVGVLLITYFPPLTTFLPRFF
                     K"
     gene            2994..3569
                     /locus_tag="VIL39_04190"
     CDS             2994..3569
                     /locus_tag="VIL39_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5480655.1"
                     /translation="MERTDAQLIAAVLKGDAASFEPLVEKYSPRIFATARRYARRESE
                     VEDLVQTIWLKAFEKLKSFRREAPFEHWLMRLAVRTCYDGLRAHQRNRETTFSELSQP
                     EEDWLDRFVSQPDAASEDAEAARQLVARVLEQLSPPARLVITLQEIEQRSVKEISELT
                     GWSVPLVKVRAFRARAEMRKVLSRIAKEKYL"
     gene            3610..3924
                     /locus_tag="VIL39_04195"
     CDS             3610..3924
                     /locus_tag="VIL39_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480656.1"
                     /translation="MNLAKLERKLIAAARGNLPSDRVPYAFEKRIMARLAARPVVDEW
                     ALWARALWRAAASCVAIMLLLGAWSFFVPPTSASANDLSQELEQTLLAAVDQDQPTDL
                     TR"
     gene            3921..4403
                     /locus_tag="VIL39_04200"
     CDS             3921..4403
                     /locus_tag="VIL39_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480657.1"
                     /translation="MNSWKVILATMVIFGTGVVTGGLVVRHAGPGGVRHPQRASGAVH
                     ATQPSPAGVMRIEFLRRMERELDLAPAQREPIDKILKDGQERTKKLMDTVEPRRREEF
                     KRTIEEFRAVLTPEQRSRFDGVVKQQQQRAREQRKAASAREHPQQSPPASNTPAATNS
                     "
BASE COUNT          743 a         1370 c         1458 g          866 t
ORIGIN      
        1 ctggccaagg gatacgtcgt cctgttctgg agtgactccg gctgggtgcg gttcttctcc
       61 aagtcgccgg tgttgcatcc ggatgacttg cggaagctga aggtcttctc cgggtcggac
      121 agcgccgccg cttatgactt gtggaaagcc ctcgggttca gcccggtatc cctggacgcg
      181 acgggcatcc cacagggcct gttatcgggc accatcagtg cggttccaac ggtgcccatt
      241 tatgcgctgg cagggcagtt ggacagccag gccaagtaca tgctggagtt gaactggggg
      301 cccctggtag gcgcggcggt cgtgcacaag aagacgtggg accgcgtgcc ggcggctgcc
      361 cgcgaagcga tgctcaagat cgccgcggaa acaggccggc aggtcagagc ggccggccgg
      421 gcggaaagcg acgccgccgt ggcggcgatg gtcaagcgcg ggctcgtggt ccagaaggta
      481 acccctgaag tggatcagga atggcgcgcg atgattgaca aggtgcaggg tcaaatccgg
      541 ggcaaagtcg tgcccgcaga cctgtttgat gaagcgcagc ggtttgcaaa agactatcgt
      601 gccgcgggcg gcaaaggagt tgagaagaaa tgaagggggc ctcctttccc gcagagttta
      661 gtcgccaaac ccgaaggtcg aaggccgaag cccgaaagaa acccgagacc cgaaggtcga
      721 accccgcagc ttgctcgcgt ccagcggcgc atgccggccg taagcgcttc ggcctgtgtg
      781 ccttctctcc tgaaaccccg gcggcctcag cccaagcgac gatctgttcg ggccttcggg
      841 cttcggcctt ctttcggcct tcggccctcg ggtttcggat ttcccgagcc acgcgccttc
      901 ccactaaaac cgcacgagga gagagttgtc cgtgagcagc ggcgccgttc tccctcccga
      961 cctgctaggt tcgcccgagt caacgacgct ctcccgccgg cggcgcctgt tctgccaggg
     1021 tgaaaacctc ctgctggtga ttccattggc ggcgatgctg ctgctgcccg tcgtggaaat
     1081 catcttgcgc ctggtgttca agaccggcat ctcgggttcc agcgccattg tgcagcacct
     1141 gacgttgatt gtgggaatgc tgggcggggc catcgcggcg cgggaccagc ggctgctcgc
     1201 gctctccccg gtgcaaacgc tgcttaaggg gcgggccaga acggcggcgc agatcttcag
     1261 cagcggcttt gcggcagcga tcaccttttt cctcttcctg gccagccttc agtatgtgct
     1321 ggatatgagg ccgctgggga aaaccctcgt ctatggcatc ccggtctggg tcatccagct
     1381 tatcctgccc attggttttg gcctgatcct gttgcgcctg ctctggcacg cctcagaaaa
     1441 atacggtgga cgggcgctgg cgctcgcgct ggccgcggca gcggtcggca tggcgatctg
     1501 ggcgccggtg tctccggaac ggctgatgat cccggcgctg gtggccttgg ggctggcgac
     1561 gctcctcggt gcgccggtct tcaccgcctt gggcggcgcg gcgctaatcc tgttttgggg
     1621 ccatgacctg ccgatccagg ccgtgccgct caaacactac agcctcacca ccaataacat
     1681 gctccccagc attcccatct ttacgctcgc gggctatttc ctcgccgagg gcggcgcgtc
     1741 caagcggctg gtgcgtgtgt ttcaagccct ggtcgggcaa ttccgcggcg ggccagcgat
     1801 cgttaccgcg ctggtgtgcg cgttctttac ctcgttcacc ggcgcgtccg gcgtcacgat
     1861 tcttgcgctg ggcggggtgc tcatgccggt cttgctggcg gcgcgctact ccgaacgcaa
     1921 cgcgctcggc ttgttgaccg gggccgggtc gctcgggatg ttgtttcccc cctgcctgcc
     1981 gctgatcctc tacacgatcg tcgccaacca cagcgcgcat gccgatctga cgatcaagga
     2041 gatgttcctg ggcggcatcg gccccgggat actgctggtc gcgctgaccg cctggtgggg
     2101 aattcgcaaa ggtcccaaag aggcgctgga ccgcccggtc ttccaatggt cggaggcatg
     2161 gaccgcgctg tgggatgcca agtgggaaat gctgatcccg gtggtagcca ttgtctcgct
     2221 gttttatgtg ccaaccaccg tcgaagcggc cgcggtaacc gccacctacg cgctcctggt
     2281 gaccacggtg attcatcgcg atctgcgtcc gatcaaagac ttgccgcgcg tgatcaccga
     2341 gtgcgggttg ctggtcgggg gcgtgctgtt gatcctgggc gtcgcgctcg gttttactca
     2401 ttacctggtg gatgcgcagc tccccgacaa gatggtcgag tgggccaccc agatggtggg
     2461 ctcgaaatgg ctcttcctgc ttggactgaa cgtggtgctg ttactcgtgg gcgggctggt
     2521 tgaaatctac gcggcgatca ttgtggtggt gcccttgctg gtgcccttgg gggtggcgtt
     2581 tggtctggac cccatccacc tcggcatcat tttcctcgcc aacatggagc tgggtttcct
     2641 ctgcccgccg gtgggcttga acctgttgct ctcctcctac cgtttcaaca agccgatggc
     2701 cgaggtcacc cgcgccacgt tcccgatgct gctggtcttg ctggtgggcg tcctgttgat
     2761 cacctatttc ccgcccctta ccacgttcct gccgcgcttc ttcaaatgat cgccgcatac
     2821 gtccagccgc agccgtacta tccgctcgtc gcggacaacg gcaagcgcct cacggcagtt
     2881 aagaatggcg cttagtttag ggcccgccgc tacctgatgg cagggtcacc ccgcgagatg
     2941 cgccttgggg gtgtgttttg actttcaggc gggggatggg catactccgc cgaatggaac
     3001 ggaccgatgc gcagttgatt gcggcggtgc tgaaaggcga tgccgctagc tttgagccgc
     3061 tggtcgagaa gtattccccg cgcatatttg ccacggcgcg gcgctacgcg cgccgcgaga
     3121 gcgaggtcga agacctcgtc cagaccatct ggctcaaggc gttcgagaag ctgaagagct
     3181 tccgccgcga ggcgcccttc gagcactggc tgatgcgcct ggccgttcgc acttgttatg
     3241 atggcctgcg ggcccaccaa cgcaaccggg agacgacctt ctccgaactg tcgcagccgg
     3301 aggaagactg gctggaccgg tttgtcagcc agccggatgc cgccagcgag gacgccgagg
     3361 cggcgcgcca gttggtcgcg cgcgtgctgg agcagctttc gccccccgcc cggctggtta
     3421 tcaccttgca ggaaatcgaa caacggtcgg tgaaggaaat ctcggaactc accggctggt
     3481 cggtgccgct ggtcaaggtg cgggccttcc gggcgcgggc cgagatgcgc aaagtcctgt
     3541 cgagaatcgc caaagagaag tatctgtaac cacacattac gacgaaccgt ctgaaaggtt
     3601 gtaggctgta tgaatcttgc caaactggaa cgaaaactga ttgccgccgc ccggggcaac
     3661 ctgccgagcg accgggtgcc ttatgccttc gagaaacgca tcatggcgcg cctcgccgcc
     3721 cggccggtcg tggatgagtg ggcactttgg gctcgcgcct tgtggcgggc cgccgcgtcg
     3781 tgcgtggcga tcatgctgtt gctgggcgct tggtcgttct tcgtcccacc gaccagtgcg
     3841 tccgcgaacg atttgtccca ggaacttgag cagaccttgc tcgcggcagt ggaccaggat
     3901 caaccgaccg atttaacccg gtgaatagct ggaaagtcat cctcgccacg atggtgatct
     3961 tcggcaccgg tgtggtaacc gggggcttgg tggttcggca tgcgggaccc ggtggggttc
     4021 gccacccaca gcgcgccagt ggggcggtgc acgccacgca accttctccc gcgggcgtaa
     4081 tgcgaatcga gttcctgcgg cgtatggagc gagaactgga cttggcgccg gcgcagcgtg
     4141 agccgataga caaaatcctc aaggacggcc aggagcgaac gaaaaagctc atggacaccg
     4201 tggagccgcg ccggcgggag gaattcaaga ggacgataga ggaatttcgc gcggtgctga
     4261 cgcccgaaca acggagccgg ttcgacggag tggtcaagca gcagcagcag cgcgcccggg
     4321 agcagcggaa ggccgcgtcg gcgcgggagc atccccagca aagccctccg gccagcaata
     4381 cgccggcagc aacaaactct taacctggag ggcgctcgcc ctccgcaagt gggaaac
//
LOCUS       DATCGS010000208         4049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_711872, whole genome shotgun sequence.
ACCESSION   DATCGS010000208 DATCGS010000000
VERSION     DATCGS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711872"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1974)
                     /gene="polX"
                     /locus_tag="VIL39_04205"
     CDS             complement(208..1974)
                     /gene="polX"
                     /locus_tag="VIL39_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY5480658.1"
                     /translation="MDKGQVAEVLVSIATLLELKGENPFKARAYLNAARTIETLSEPL
                     NKVVAEARLGDIKGIGDALQQKITELVTTGRLQYYEDLKAATPPGLIAMLEIPGLGPK
                     KIKALHDELGVETVEQLEQACKDGKIAGLKGFGEKTQANILEGINRRRSYASRHLLSD
                     ALQQAEPLLEALRSHPDVIRCSAAGSLRRHREMIGDIDLLASSKQPAEVIKFFTSQPG
                     ILKVIAKGETKASVLLEGGIQSDLRVVSDAEYSPALLYFTGSKEHNIAMRQRAIDRGL
                     RLNEYGLFHSKVETRDPKLLVKCRTEEEMFEKLGLHYIPPELREDMGEIALAEKGPLP
                     RLIEWTDLKGSLHNHSTWSDGHQRPEEIAKAMRELGLAYWGITDHSKSAFQANGLDVA
                     RVRQQLKEIASINAMLAKEGTDFRLLTGTEVDILKDGKLDFPDDLLAELDVVIASIHT
                     SFTQTEAETTQRLIGAAQNRYVHILGHLTGRLLLEREPYKVDHHAVIDACAKTGTWIE
                     LNAHPMRFDMDWRWWPYAKSKGVKCVINCDAHRFAEAGFLRLGAGIARKGWLTKEDVV
                     NTLPLKALMKELGRKRATASLP"
     gene            2313..3800
                     /locus_tag="VIL39_04210"
     CDS             2313..3800
                     /locus_tag="VIL39_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY5480659.1"
                     /translation="MTNFIQRWLRAESGASSTARRFAQRCARQGSVLGASAGVPGFLA
                     ALCLTIALFAAVPAQAVGTLNWETNRNLVSADIKSGKLIPLLEQIASVTGWHVFVEPD
                     ATCTISAKFDRLPPGEALHLLLHDLNFALVPATNASAQLFVFHSTMRNATQSVRPANP
                     AGADSKGKLIPNELIVRLKPGAKIDDLAKLLGAKVTGRIDGLNAYRLQFEDQAAADAA
                     RAQLTSNPDVASVDSNYSIDRPTTPAGAQGANLPPPPQLQLKPPLSDGRIIVGLVDTA
                     LQPLGNGLDSFLLKAISIAGDAQLDPNSPSHGTSMAETILRSLAAMTKGSSSVQILPV
                     DVYGPNASTTTFDVANGIAQAVNGGANPINLSLGSAGDSPFLDSIIQDARSKNIVFIA
                     AAGNTPVTTAFYPAAYSGVMAVTAIDQGQLASYANHGSFVSLGAPGTSIIYFNNQAYY
                     VMGTSASAAFISGIAAGYMDSTHNSSSQMQNFLRSNFGVTIVPAK"
BASE COUNT          707 a         1286 c         1226 g          830 t
ORIGIN      
        1 caagcaggaa cccgcgaaac acgcgacacg aaggcggagg gtgggcgcta acacgcgaag
       61 cgtttggagt gcgtccagct tactggcgct gtcgtaaggc gagggggttt gaaagcggga
      121 gcaagctcct cgcactccaa acgcttcgcg caattcggtg gcggctttgc cgcgctgcgt
      181 ccatcgcggc tcccttcaac tatagggtca cggcaaagag gcggttgccc gtttccggcc
      241 cagctctttc atgagcgctt tcaggggcag agtgtttacc acatcctctt tcgtcagcca
      301 gcctttgcgg gcgatgccgg cgccgaggcg gaggaagccc gcctctgcga agcggtgcgc
      361 gtcgcagttg atgacgcact tcacgccctt ggatttggcg taaggccacc agcgccagtc
      421 catgtcgaag cgcatcgggt gggcgttaag ctcgatccag gtgccggtct tggcgcaagc
      481 gtcaattacg gcgtggtgat ccaccttgta gggctcgcgc tccagcagca ggcggcccgt
      541 gagatggccg aggatgtgga cgtaacggtt ctgcgccgcg ccgatcagcc gctgggtagt
      601 ctccgcctcg gtctgggtga agcttgtgtg aatgctggca atcacgacat ccagctccgc
      661 cagcaggtcg tccgggaaat cgagcttgcc gtcctttaga atgtccacct ccgtgccggt
      721 gagcaggcgg aagtcggtcc cctccttcgc cagcatggcg ttgatggagg cgatttcctt
      781 gagctgctgc cgcacgcgcg ccacgtcgag gccgttggcc tgaaaggcgg acttcgagtg
      841 atccgtgatt ccccagtagg cgaggccgag ttcgcgcatc gctttggcaa tctcctcggg
      901 gcgctgatgc ccatcgctcc aggtggagtg gttgtgcagg gagcccttta gatccgtcca
      961 ctcgatcagg cgcgggagcg gccccttctc cgccagcgcg atctcgccca tgtcctcacg
     1021 cagctccggc gggatatagt gcaggccgag cttctcgaac atctcctctt cggtccggca
     1081 tttgacgagc agcttcgggt cgcgcgtttc caccttggaa tggaacaagc cgtattcgtt
     1141 cagccgcaag ccgcgatcaa tcgcgcgctg gcgcatggcg atgttatgct ccttgctgcc
     1201 ggtgaaatag agcagggcgg gggaatactc cgcgtcgctg accacgcgca ggtcggattg
     1261 gatgccgcct tcgagcagca cgctggcctt ggtttcgccc ttggcaatga cctttagaat
     1321 gccgggctgg ctggtgaaga acttgatgac ctccgccggc tgcttcgagg aggcgagcaa
     1381 gtcaatgtcg ccaatcatct cgcgatgccg ccgcaggctg cctgccgcgc tgcaacggat
     1441 cacgtccgga tgcgaccgca gcgcttcgag cagcggctcc gcctgctgga gtgcgtcact
     1501 caggaggtgg cgcgaggcgt aggagcggcg gcgattgatg ccttcgagga tgttcgcctg
     1561 tgttttctcc ccgaaccctt tcagcccggc gatcttgccg tccttgcagg cctgttcgag
     1621 ctgctcgacc gtttcgacgc ccagttcgtc gtgcagggct ttgatcttct tcggacccag
     1681 cccggggatc tcaagcattg cgatcagtcc gggcggggtc gccgctttca agtcctcgta
     1741 atactgcaag cggcccgtgg tgacgagttc ggtgatcttc tgctggagcg cgtcgccaat
     1801 gcccttgata tcgcccaggc gcgcttcggc gacgaccttg ttcagcggct cgctcagcgt
     1861 ctcaatggtg cgggcggcgt tgagataagc gcgggccttg aacgggttct cacccttcaa
     1921 ctccagcaac gtcgcgatac taaccaacac ttcggctacc tgacccttat ccatgcctga
     1981 aatatggcgc tcaacgtccc aggggcaaag cgcaaaatgc gccgcccgat cgccgacagc
     2041 agcggcgcca caagtcaggt gcaagcggga tacacacgcg atgctcgcgt gttgctatcg
     2101 tatttccttc gtattctctt cgtattctct tcgtgtttcc ttcgttttct tgtccctttc
     2161 tcccgtcggt tcggcctgcc ttccgcggcg gaggtgccgt tacgcttgcg cgttgtcttt
     2221 ctggcttcat cctgctactt ttgcccttgc tgattggcaa aagcgggcga ttttgaatgg
     2281 ttgactacga aaaccagtct gattaggcga gcatgacgaa cttcatccag cggtggttgc
     2341 gagcagagtc cggagcgtcg agtacggctc ggcggttcgc gcagagatgt gcccggcagg
     2401 gcagcgtcct cggcgcttcg gccggggtgc cgggtttcct ggctgccttg tgcctcacaa
     2461 ttgcgttgtt cgctgccgtc cccgcccagg ccgtcggcac tcttaactgg gagaccaacc
     2521 ggaatctagt tagcgcggac atcaagtccg gcaagctcat cccgctgctc gagcaaatcg
     2581 cctcggtcac cggctggcat gtgtttgtcg aaccggacgc gacctgtact atctcggcca
     2641 agttcgaccg tttgccgccg ggcgaggcgc tgcacttgct gctgcacgat ctgaacttcg
     2701 cgctggtgcc tgcaaccaat gcgagcgcgc agctcttcgt cttccactcc acgatgcgga
     2761 atgcgacgca atccgtgcgt cccgccaacc ccgcgggggc cgactccaag ggcaaactga
     2821 tccccaatga actcatcgtt cggctcaagc ccggtgcgaa gattgacgac ctggccaagc
     2881 tgctgggtgc taaagtcacc ggtcgcatcg acggcttgaa cgcctatcgc ctccagttcg
     2941 aggaccaggc tgcggcggac gccgcccgcg cgcagctcac ctcaaatcca gatgtcgcct
     3001 cggtggacag caactactcc attgaccgcc cgacgacgcc cgctggggcg cagggggcga
     3061 atttgccgcc gccgccgcag ttgcagctta agccgccgct cagcgacggc cgcatcatcg
     3121 tgggcttggt tgataccgcc ctccagcccc tcggcaacgg tctggacagc ttcctgctca
     3181 aggcgatctc catcgcgggc gacgcccagc tcgacccgaa cagccccagc cacggcacct
     3241 cgatggctga aacgattctg cgaagcctgg ccgccatgac caaaggcagc tcctcggtgc
     3301 agattctgcc ggtggatgtc tatggcccga atgcgtccac caccacgttc gacgtcgcga
     3361 acgggattgc gcaggccgtg aacggcgggg ccaaccctat caatcttagc ctcggcagcg
     3421 ctggcgatag cccttttctc gatagcatca tccaggacgc cagatcgaag aacattgtgt
     3481 ttatcgccgc cgccggcaac acaccggtta ccacggcgtt ttacccggcg gcctattcgg
     3541 gagtgatggc ggtgacggca attgatcagg gccaacttgc cagctatgcc aaccatggca
     3601 gcttcgtctc cctcggcgcg cccggcacca gcatcatcta cttcaacaac caggcgtatt
     3661 atgtgatggg gacttcggcc tcggccgcct tcatcagcgg catcgcggcc ggctacatgg
     3721 actcgacgca caatagctca agccagatgc agaacttcct gcgcagcaac ttcggggtga
     3781 ccatcgtccc ggccaagtga acggcgagac gtctttgccg cctgggcggc gcgacgggtc
     3841 acaaccgagc gcgcccttcg atctccagca caaggcgggc ttctgtagcc tttcctgccg
     3901 cgggtgcagg actgaattct tcaatagccg tgatagaaga gcatcggagc acctgctgta
     3961 gatgggaact atatagagcc cgtcccaaca gtcacaagtg ccataggcac aatgggttac
     4021 tccggctagt gcgagaataa acccggttg
//
LOCUS       DATCGS010000209         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_68541, whole genome shotgun sequence.
ACCESSION   DATCGS010000209 DATCGS010000000
VERSION     DATCGS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68541"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..1938
                     /locus_tag="VIL39_04215"
     CDS             562..1938
                     /locus_tag="VIL39_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5480660.1"
                     /translation="MRLEVNESPVARDSKFTTSFAPVVKKVSPSVVKVFVTAKANPAA
                     LSGRGMPDLRRYFGDEENGPDSPRPFGSPNQHGVGSGVIVTKDGYILTNNHVVESADD
                     IKVALNDGREFSAQVIGRDPQTDIAVLKIDARELPFVTLADSDKIEVGDVVLAIGNPF
                     GIGQTVTTGIISAKGRATLGLDYEDFIQTDAAINPGNSGGALVDTDGRLIGINTAILS
                     RSGGNQGIGFAVPTNLARWVMEGLVQNGRVERGFLGVNIQDLTPQLAKEFKLDHANGA
                     LVSEVTPQSPADKGGLKNGDVIVEFNGTPVTDSRHLKLQVGATIAGSSVPVKILRDGA
                     SKTLQVTIEKLASDKLAKASSPADQAEDALHGVAVADLDLASRAELKVPERVNGALVS
                     QVQPDSAAYEAGLRAGDVITEINRQPVKNSAQAVAACEKPADKQMLVKVWSHGGSRYV
                     VVDETKAS"
     gene            2043..>2344
                     /locus_tag="VIL39_04220"
     CDS             2043..>2344
                     /locus_tag="VIL39_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5480661.1"
                     /translation="MRLLVVEDDSKIASFVVKGLKQNGFAVDPASDGEKGLELAQAVT
                     YDAAVLDIMLPKLDGLSLLRQLRQDKILTPVLILSAKASVDDRVKGLQAGGDDYLT"
BASE COUNT          499 a          737 c          686 g          422 t
ORIGIN      
        1 atgcggtcaa tccgtttgtc tacatgatgg ccgtggtcgg gaatgctccg gctgccaagt
       61 ccgccccgga acagtggatg ccctggaact atccagccaa cccagatcaa caaacagcgg
      121 ccaccccttg accgtcctca aggcaaaata tcgccctgcc gcaatgctcg cgatcagttt
      181 tatgagccct ccgcacgctt gccgaaggca cacgatgaaa cccgccgtcg agttgatcgt
      241 ggatgccgcc cgctgccact tcctgcgtca tccatgcccg agcgtcacct ctccctcgtt
      301 cgaccaggcc cgccgcctta cacacttgta atctgtcagc aaggccccgg tcaggttggt
      361 ggggtctatt gctatcagaa agcggtaaaa cgccgcgatc caaacgaaca cagaaaagat
      421 aaaaaacata tgaaacacac atcccctcac ccgttctcct ggcggcgcac ggcgctgctc
      481 ggaggcgccg catggttatt agccacctcg ccggggttca ccccgtgagc ccacgcgaag
      541 gacaattcga aggttgcccc cttgcgcctt gaagtcaacg agtcgcccgt cgcgcgcgat
      601 agcaagttca ccacaagctt tgcacccgtg gtgaaaaagg tctccccgag cgtggtcaaa
      661 gtatttgtca ccgccaaagc aaatcctgct gccctgtccg gccgcggaat gccggacctg
      721 cgtcgctact tcggcgatga agagaacgga ccggattctc cgcgcccgtt cggttccccg
      781 aatcaacacg gtgtcggcag cggcgtcatc gtcaccaagg atggttacat tctcacgaac
      841 aaccacgtcg tggagagtgc cgacgacatc aaggtggcgc tcaacgatgg ccgtgagttt
      901 tccgcccagg tcatcggccg agatccgcaa accgacatcg cggtgctgaa gattgacgcg
      961 cgcgagctgc cgttcgtcac tctggccgac agcgacaaga tcgaggtggg tgacgtggtg
     1021 ctcgccatcg gtaatccttt cggcattggc cagaccgtta cgaccggcat catcagcgcc
     1081 aagggccgcg ccaccctggg cctcgattac gaggatttta tccagactga tgcggccatc
     1141 aaccccggca attccggcgg cgcgctcgta gacacggacg gccggctcat cggcatcaac
     1201 accgccatcc tcagccgaag cggcggcaat caaggcattg ggttcgccgt gccgacgaat
     1261 ctggcgcgct gggtcatgga gggtcttgtg cagaatggac gcgtcgagcg tggtttcctc
     1321 ggggtgaaca ttcaggatct cacgccgcag ctcgcgaagg agttcaagct cgaccacgcc
     1381 aacggcgcgc tggtgtcgga agtcacgccg caaagtcccg ccgacaaagg tggattgaag
     1441 aacggcgatg tgatcgtgga attcaatggc acgccagtga cggacagccg tcacttgaag
     1501 ttgcaggttg gcgcgacaat agccggttcg tccgtgccgg tgaaaattct gcgcgacggc
     1561 gcgagtaaga cgctccaggt caccatcgag aaattggcca gcgacaaact cgcgaaagct
     1621 tcctcgcccg ccgatcaagc tgaggacgca cttcatggcg tggccgtggc cgatctcgac
     1681 ctggccagcc gcgcggaact caaagtgccg gagcgcgtca acggcgcgct cgtctcccaa
     1741 gttcaacccg actccgccgc gtacgaagcc ggcctgcgcg cgggcgatgt gattacggag
     1801 atcaaccgcc aaccggtaaa aaactccgcg caagctgtcg ccgcctgcga aaagccggcg
     1861 gacaaacaga tgttggtcaa agtctggagt cacggcggca gccgctatgt cgtcgtggac
     1921 gagaccaagg ccagttgaaa tggctgacga ccgcttcgcc ctcctgccca cgagccggag
     1981 ggcgaatccc tttaaagctt tctttgcgcg aaggtatcgg atacgctcgc ccgccttgaa
     2041 ttatgcgcct gcttgtagtt gaagatgaca gcaagatcgc ctcgttcgtc gtcaaggggc
     2101 tgaagcaaaa cggcttcgct gtggaccctg cgtccgatgg tgaaaaaggg ctcgagctgg
     2161 cgcaggcggt cacctacgac gcagcggtgc tcgacatcat gttgccgaag ctcgacgggc
     2221 tttcgttgct gcgccagttg cgccaggaca aaatcctcac gccggtgctg atcctcagcg
     2281 cgaaagccag cgtggacgac cgcgtgaaag gcttgcaggc gggcggcgac gattatctca
     2341 ccaa
//
LOCUS       DATCGS010000210         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_663177, whole genome shotgun sequence.
ACCESSION   DATCGS010000210 DATCGS010000000
VERSION     DATCGS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663177"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..571)
                     /locus_tag="VIL39_04225"
     CDS             complement(185..571)
                     /locus_tag="VIL39_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5480662.1"
                     /translation="MNVVDSSGWIEYFTAGPNSGFFAGAIENVDELIVPALAICEVFK
                     WVSRERGETQALKAIAHMQLGEVVELDSGLAIYAARLSLQSKLPMADSVVYATARAHE
                     ATLWTQDDDFEGLDDVKYIAKKKTAR"
     gene            complement(568..774)
                     /locus_tag="VIL39_04230"
     CDS             complement(568..774)
                     /locus_tag="VIL39_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY5480663.1"
                     /translation="MNTVTLSPKFQVVIPQPVRDFLKLKPGQKLQVFALGGRIEIVPL
                     QPMKEMRGFLKGMNRDFEREPDRL"
     gene            complement(999..1202)
                     /locus_tag="VIL39_04235"
     CDS             complement(999..1202)
                     /locus_tag="VIL39_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480664.1"
                     /translation="MREEPRAPTRPDKCPRLSWLPPAKQGVIDITVPYFQKHGAYIQE
                     HGVWLEGKRISKKLPVSLRMPNP"
     gene            complement(1342..>2854)
                     /locus_tag="VIL39_04240"
     CDS             complement(1342..>2854)
                     /locus_tag="VIL39_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEY5480665.1"
                     /translation="ERFLEENWLFEAVTETYVPLLQLLQRWQRDGLQPRLTLTLTPTL
                     CSMLLDPLLQGRYERHLNELIGLAEKEIHRTQFERTFHQLAQFYHGRFTAVRDAYAAC
                     GRDLVGAFRQFQERGYLEIITSAATHALLPLLADHPPSLRAQVLVARDHYRSCFGCEP
                     RGIWLPECAYVESLDDVLQEANLHWFVTDTHGILHARPQPLYATFAPIFTPNGIAAFG
                     RDPDSTRQVWSRHTGYPGDPRYRDFYRDIGFDLDWDYVKPHLPAPDSRGFTGIKYHRI
                     TGSSPDKAAYDRQAAIRAVESHAAHFLAARIAQFRQLAETLDRPPIVVAPYDAELFGH
                     WWYEGPEFLDSLVRKACRDQKVFELITPAEFLRQHPTHQVATPAASSWGEEGYWRVWL
                     NEKNEWIYPHLHVAQDRMTELARRFPQPDALQERMLKQAARELLLAQASDWPFILQTG
                     TSPGYARQRVQDHLLRFTRIYEQLTAAQPDPEWLKQVEWRDNIFPDVDYRYWT"
BASE COUNT          522 a          860 c          891 g          581 t
ORIGIN      
        1 tgtttgcccc gttcggtcgc acgatgcgga catgggacca cgcccgtcat tacgacgcgt
       61 agtgcctttt ttgcctcatt gacgccaatc cgcctggcac tcaattttcc agcatgagcg
      121 cggttgctgg aatcgttttc ccgaaccgca catcgttaat gccgccaagc tccaccagga
      181 gctttcatct cgccgttttc tttttggcga tgtatttcac atcgtcgagt ccttcaaaat
      241 catcgtcctg cgtccacagc gtcgcctcgt gggcgcgggc ggtggcataa acaacggaat
      301 ccgccatggg cagtttggac tgcaggctca agcgtgcggc gtaaatggca agtccggaat
      361 ccagttccac cacttccccc agttgcatgt gggcgatagc cttcagcgcc tgggtttcgc
      421 cgcgttcgcg ggagacccat ttgaaaacct cgcagatggc cagcgccgga acgatcaatt
      481 cgtccacgtt ttcgattgcc ccggcgaaga agccggagtt cgggccggcg gtgaaatact
      541 caatccatcc ggatgaatcc acgacgttca caggcggtcg ggttcgcgct caaagtcgcg
      601 gttcatgccc ttcaaaaaac cgcgcatctc cttcatcggc tgcaggggaa caatttcaat
      661 ccgcccgccc agcgcaaaga cttgcaactt ctggccgggc ttgagtttta ggaaatcccg
      721 caccggctgc ggaatgacca cttgaaattt cggcgacagg gtaacggtat tcatatcgtt
      781 atcagttcgg tatgacgtta tgttcttcga tgagttatgt caaccacgcg gattaagatg
      841 cttttccggc aatctggaaa aggacttggc cgcttgaagc ctctggatct aagcgggtcg
      901 ttcctcgcgc gcgtccgcgc ttatcccaaa gagacacgcg gcagaattgg acgggcgttg
      961 cagcgcttgg agcgggattt cggccatccg catcgtcatc agggattcgg catccgcaag
     1021 ctgacgggaa gcttctttga aatccgcttc ccttccaacc acaccccgtg ctcctggatg
     1081 tacgcgccgt gcttctggaa gtagggcacg gtaatatcaa tcacgccttg cttggccggc
     1141 ggcagccagc tcaggcgcgg gcacttgtca ggccgggtcg gcgctctggg ctcttcgcgc
     1201 acggccttcg ttgcctcgcc taccccgcat aggcatccct cccggccgcc ttctgcctgt
     1261 ccggctccgt caccctgtcg ttatgggtct tcatagagtt ccattctcaa tcgaacgccc
     1321 cattttgcca tcccgctgcg gctaagtcca ataccggtaa tcaacgtcgg ggaagatatt
     1381 gtcccgccac tcgacttgct tgagccattc cggatcgggc tgagccgcgg tgagttgctc
     1441 gtaaattcgg gtgaagcgca ggaggtgatc ctgcacgcgc tggcgggcgt agccaggact
     1501 cgttcccgtc tggaggataa acggccaatc gctggcttgc gcgaggagga gttcgcgggc
     1561 ggcttgctta agcatgcgct cctgcaaggc gtccggctgc gggaagcgcc gcgccagttc
     1621 cgtcatgcga tcctgcgcca cgtggagatg ggggtagatc cattcgttct tctcgttgag
     1681 ccagacgcgc caatagcctt cctcgcccca gcttgaggcg gccggcgtgg ccacctggtg
     1741 cgtcggatgc tggcgcagga actccgccgg cgtgatcagc tcgaacacct tctggtcgcg
     1801 gcaggccttg cgcacgaggg aatcaaggaa ctccgggccc tcgtaccacc agtgaccgaa
     1861 caattcggcg tcatagggtg caaccacgat cggcggacgg tcgagggttt ccgccaactg
     1921 ccggaactgc gcgatccgag cagcaagaaa gtgggccgcg tggctttcca cagcccggat
     1981 ggcggcttgg cggtcatacg cggctttgtc gggcgagctg ccggtaatgc gatggtactt
     2041 gatgccggtg aacccgcgtg agtcgggcgc gggcagatgc ggcttgacgt agtcccaatc
     2101 gaggtcgaag ccgatgtcgc ggtagaagtc gcggtagcgc gggtcgcccg ggtaaccggt
     2161 gtgccggctc cacacttgcc gggtggaatc gggatcgcgg ccaaaggcgg cgatgccgtt
     2221 gggtgtgaag atgggtgcaa acgtcgcgta aaggggctgg ggccgcgcgt gcagaatgcc
     2281 gtgcgtgtca gtaacgaacc agtggaggtt ggcctcctgc aatacatcgt ccaggctttc
     2341 cacataagcg cattccggca gccagatgcc ccggggctcg cagccaaagc agctccggta
     2401 atggtcgcgc gctaccaaca cctgcgcgcg cagggaaggc ggatgatcgg ccagcagcgg
     2461 caggagcgcg tgcgtggcgg cgctggtgat gatttccaaa tacccccgct cctggaactg
     2521 gcggaaggcg cccaccaggt cgcgcccgca ggctgcgtaa gcgtcacgaa cggcggtaaa
     2581 gcgcccgtga tagaactggg cgagttggtg gaaggtcctc tcgaactggg tgcgatggat
     2641 ctccttctcc gccagtccga tgagttcgtt gaggtgccgc tcgtagcggc cttgcaggag
     2701 cgggtccagc agcatcgagc agagcgttgg cgtgagcgtg agcgtgaggc gaggctgcaa
     2761 gccgtcccgc tgccagcgct gcagcagctg cagcagcggc acgtaggtct ccgtaacggc
     2821 ctcgaacagc cagttctctt ccagaaaccg ctcg
//
LOCUS       DATCGS010000211         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_266355, whole genome shotgun sequence.
ACCESSION   DATCGS010000211 DATCGS010000000
VERSION     DATCGS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266355"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIL39_04245"
     CDS             complement(<1..221)
                     /locus_tag="VIL39_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5480666.1"
                     /translation="MKITILGAGAWGTALVRLLHQGGHGITLWGHKAEWLAEIRQTGR
                     NDRFLPGIEVPREVALEADLPCAIEGAEC"
     gene            complement(218..907)
                     /gene="plsY"
                     /locus_tag="VIL39_04250"
     CDS             complement(218..907)
                     /gene="plsY"
                     /locus_tag="VIL39_04250"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY5480667.1"
                     /translation="MVGYILVVLIAYLLGSIPTGFLVAKARGVDIRSVGSGNIGATNV
                     FRVLGKSAGAFVLLADAAKGWLAVFVVARLVSGWFYPDAGSLAREWFSLCAGIAAILG
                     HNYTCWLHFKGGKGIATSAGVLVALVPVPLLIILGVWIIVFALSRYVSLASISAAFTL
                     PFAVWLVGEIRDKIQGDRDVTRIIVLITAALAALAIYKHKTNIQRLLKGTENRIGAKK
                     PLPSDASKPIA"
     gene            1027..2091
                     /locus_tag="VIL39_04255"
     CDS             1027..2091
                     /locus_tag="VIL39_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480668.1"
                     /translation="MNTDDEPRTTDIVKVSVLGTGSLGKEHVRIYSELAAAGKVEFAG
                     VYDTVAETAQRLAQKYGIRAFGSVAEAAAASDAVSIVTPTTTHFDLAKTLLQQSRHVL
                     VEKPMTENAAQATELVQIAQQKHCVLQVGHVERFNPVFKYLESVATDPRFIETHRLSP
                     FPARSTDIGVVLDLMIHDLDVVLAFVKSPVTSVDAVGIPVLSKSEDIANARLRFANGC
                     VANLTASRVSPERMRKIRVFSGGAMTSYISLDYRAQEGFIYRIAKDGEEESSLLKKLL
                     HAKDSTIVSEFGGKRIVREPVPIAKEEPLKLELQSFVQCVRAHRTPVVSGESAKRALD
                     LAFEITRQIQTAALNPETPG"
     gene            2091..3314
                     /gene="lpxB"
                     /locus_tag="VIL39_04260"
     CDS             2091..3314
                     /gene="lpxB"
                     /locus_tag="VIL39_04260"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY5480669.1"
                     /translation="MKPTSFMLIAGEASGDMLAAELVRALRRAVPAATPISTTDYQPL
                     HGSLEPRFFGAGGPQMAAAGVELAFDMTAHSVIGLSEALKNYLKFRRLFRQLFRLALD
                     RQPDVIICVDFSGFNRRFAHAIRQYTRARADWFHNWHPKIIQYVSPQVWASREGRAYQ
                     IARDYDLVLSIFPFEKAWYAKRVPQLRVEFVGHPIVERYGQARTVRGETRGNKGTPMA
                     LLLPGSRPDELRRHLPVMLAALGLIRAKVPNLRARMVLPNEGFIQQARTFKPPTSLAM
                     QAGGLPEALAEADVALASTGTVTTECAYFGVPTVALYKTSWSTWQIARHIVKVKYAAM
                     PNLLADEEIFPEFIQDAATPENLARAAVELLGNEGRRARVKARLAEIIAALGGPGATQ
                     QAAQAVMATLKAEAS"
     gene            complement(3397..>4086)
                     /gene="recO"
                     /locus_tag="VIL39_04265"
     CDS             complement(3397..>4086)
                     /gene="recO"
                     /locus_tag="VIL39_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY5480670.1"
                     /translation="QRCARRFALALGSRPPMIETAMGLVFRTRLLTETSLIVQWLTPN
                     LGRLATVAKGARRAKSPFRGKLDLFYLADFSFSRSSRSELHTLREVSLRETHSGLRKD
                     LGLLQQASYCAALVERATETETPLPQVFDLMVGLMQQLLTHPPQPQTVFSFELKLLAE
                     LGLKPDLAKSQLNPGTRQLVKALAENDWPVMSRLKLSHEQVAELRQFLHGFLQYHLGS
                     LLKGRAGALGL"
BASE COUNT          828 a         1249 c         1292 g          717 t
ORIGIN      
        1 atgcactcag ccccctcgat ggcgcagggc aggtcggcct ccaaagcgac ctcgcgcggc
       61 acctcgatgc ccggaagaaa gcggtcgttg cggccagttt ggcggatttc agccagccat
      121 tccgctttat gcccccagag cgtaattccg tgtccgccct gatgcagcag ccgcaccaga
      181 gctgtccccc atgcgccggc gccgagtata gtgattttca tgcgattggc ttcgacgcgt
      241 ccgacggcaa cggcttcttc gcgccgattc ggttctccgt gcccttgagc aggcgttgga
      301 tgttggtctt gtgtttatag atagccagcg cggccagcgc ggcggtgatg agaacaatga
      361 ttcgggttac gtcgcgatcg ccctgaatct tgtcccggat ttcccccacc aaccaaacgg
      421 cgaacggcag cgtgaacgcg gcggatattg aggcgagtga cacgtaacgg ctcagtgcaa
      481 agacgataat ccagaccccc aggatgatca gcagcggcac cggcacgagc gcaaccagca
      541 ccccagcgga ggtggcaatg cccttgccgc ccttgaaatg gagccagcag gtgtagttgt
      601 gtccaagaat agccgcaatg ccggcacaaa gcgaaaacca ctcgcgcgcc aatgagccgg
      661 cgtcagggta gaaccagccg gacaccagcc tcgccacgac aaagacggcc agccagccct
      721 tggcggcatc ggccagcaga acgaaggcgc ccgctgattt gcccaggacc cggaatacgt
      781 tggtcgcccc aatattgccg ctgcccacgc tgcggatatc gacgccccgg gcctttgcga
      841 cgaggaagcc cgtggggatc gacccaagca agtaggcgat cagcacgacc aggatgtaac
      901 caacaatcgg cacgaggaca ctctatgaat ccagggcgca aagttcaaag tccaatttgg
      961 ggacgacaga ccgacttgcc agttgcgagt tgtcagttgg catctgccaa tggcacaatg
     1021 gcggccatga acacggacga cgaaccacgg actacggaca tcgtaaaggt gtccgtcctc
     1081 ggcaccggct cactcggcaa ggagcatgtg cggatttatt cggagctggc ggcggctggg
     1141 aaggtcgagt ttgcaggggt ttacgacacg gtggccgaaa ccgcgcagag actcgcccag
     1201 aaatacggga tccgggcgtt cgggtcggtg gccgaggctg cggcagccag tgacgccgtg
     1261 agcatagtaa cgcccaccac gacgcatttc gacctggcca aaacgttgct gcagcagagc
     1321 cggcatgtgc tggtcgaaaa gccgatgacc gagaatgccg cccaggccac cgagctggtc
     1381 cagatcgcgc agcagaagca ttgcgtcctg caggttggcc acgtcgagcg gttcaatccg
     1441 gttttcaaat accttgagag cgtggccacc gacccgcgct tcattgagac tcaccgcctt
     1501 tcgccgttcc cggcgcgcag cacggatatc ggcgtggtgc tggacctaat gatccacgat
     1561 ctggacgtgg tgctcgcctt cgtgaagtcg ccggtgacca gcgtagatgc ggtgggcatc
     1621 ccggtgttga gcaagtcgga ggacatcgcg aacgcgcggc tgcgctttgc caacggctgc
     1681 gtggccaacc tgaccgcgag ccgcgtcagc ccggagcgga tgcgcaagat ccgcgtgttc
     1741 agcgggggcg cgatgaccag ctatatctcg ctcgattacc gcgcgcagga ggggttcatc
     1801 tatcgcatcg cgaaggatgg cgaagaggag agctcgctgt tgaagaagct gctccacgcc
     1861 aaggactcca cgatcgtcag cgagttcggc ggcaagcgga tcgtgcgcga gccggtgccg
     1921 attgccaaag aggagcccct caagctggag ctgcagagtt ttgtccagtg cgtgcgcgct
     1981 catcgcaccc cagtcgtcag cggcgaatcg gcgaaacgag cgttggacct ggcgtttgaa
     2041 atcacccggc agatccagac ggcggcgctt aacccggaga cgcctggtta gtgaaaccaa
     2101 cgagcttcat gctgattgcg ggcgaggcta gcggcgatat gctcgccgcg gagttggtgc
     2161 gcgccttgcg ccgggcggtc cctgccgcca cgccgatttc caccacggat taccagccgc
     2221 tccacggcag cctggagccg cggttcttcg gtgccggcgg gccacagatg gccgctgcgg
     2281 gagtagagct ggcctttgac atgacagcgc attcagtgat tggcctctcc gaggcgctga
     2341 agaactacct caagttccgc aggctgttcc gccaactctt ccggctcgcg cttgaccgcc
     2401 agcctgacgt catcatctgc gtggatttct ccggcttcaa ccgccgcttt gcccatgcca
     2461 tcaggcaata cacgcgggcg cgagccgact ggttccacaa ctggcaccct aagattatcc
     2521 agtacgtctc accgcaagtc tgggcgtcac gtgagggccg cgcctaccaa atagcgcgcg
     2581 actacgacct ggtcctgagc atcttcccgt tcgagaaagc atggtatgcg aagcgcgtgc
     2641 cacagttgcg tgtggagttt gtcggccatc ccatcgtcga aaggtacggg caagcgcgaa
     2701 cggtcagggg cgagacacga gggaacaagg gaacgccgat ggcgctgctg ctgccaggca
     2761 gccgaccgga cgagttgcgc cgccatctgc cagtgatgct cgccgccttg ggcttgatcc
     2821 gcgccaaagt tccaaatctg cgcgcacgca tggttctgcc caacgagggt ttcattcagc
     2881 aagcgaggac cttcaagcct ccgaccagtt tggcaatgca agccggcggt ttgcccgaag
     2941 cattggccga agcggacgtg gcgctcgcct cgactggcac cgttacgaca gagtgcgctt
     3001 acttcggcgt gccgactgtg gccctctaca aaacgtcctg gagcacctgg caaatcgcca
     3061 ggcacatcgt aaaagtgaaa tacgcggcca tgcctaactt gctggccgac gaggaaatct
     3121 tcccggagtt tatccaggac gctgctacgc cggagaatct cgcccgggcg gcagttgagc
     3181 ttctggggaa tgaaggccgc cgcgccaggg tcaaagccag gctggctgaa atcatcgccg
     3241 ctttgggtgg tccaggcgca acacagcagg cagcgcaggc cgtaatggcg acgctgaagg
     3301 ctgaagcgag ttagcgacca cggctgactg gcccgagtct gttggccgaa ggcaccggtt
     3361 cagacagaga cacgcccgcg cggcacacct gaagagttac agccccagcg cccccgcgcg
     3421 ccctttcagg aggctcccca gatgatactg caagaacccg tgcaggaatt gacgcagctc
     3481 ggcaacctgc tcatggctga gtttcaggcg tgacattacc ggccaatcgt tttccgcgag
     3541 cgccttcacg agctggcggg tgccaggatt cagttggctt tttgccaggt ccggcttcag
     3601 cccgagctcg gccagcagtt tgagttcaaa ggagaagacg gtttgcggct gaggcggatg
     3661 ggtcaagagt tgttgcatca agccgaccat caagtcgaag acctggggca acggcgtttc
     3721 cgtctcggta gcgcgttcga ccaaggcggc gcagtaggaa gcttgctgaa gcaggccaag
     3781 gtctttacgg aggccgctgt gcgtctctcg caggctcacc tcgcgcaggg tatgaagctc
     3841 ggaacgcgag ctgcggctga agctgaagtc cgccaggtaa aacaagtcca gtttgccgcg
     3901 gaacggtgat ttggcccgtc gcgcgccttt ggccactgtc gccagccggc caaggttggg
     3961 cgtgagccac tgcacaatga ggctggtctc ggtgagcaac cgggtgcgga agaccaggcc
     4021 catggcggtt tcgatcattg ggggccgact cccgagagca agggcaaacc gccgcgcgca
     4081 cctttg
//
LOCUS       DATCGS010000212         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_415650, whole genome shotgun sequence.
ACCESSION   DATCGS010000212 DATCGS010000000
VERSION     DATCGS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415650"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VIL39_04270"
     CDS             <1..941
                     /locus_tag="VIL39_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5480671.1"
                     /translation="TTNKVMRDVIHDVCTRVESGDSFSEALAKHPKAFSRLYICMVAA
                     GEQGGLLAEILARLATYLETTARLRKKIKSAMMYPAVVSVVAVLITTFLLIKVVPVFG
                     EIFASFGAKLPAPTAYLIMLSNIVKHYLIFILLAGGGVVYGWFYFIKTPAGRLFWDSR
                     RIKLPIFGAIAHKICLARFTRTLSSLVRSGVPILEVLQIVSQTVGNVVMEKAIKTAAS
                     DIERGESISAALAKHPIFPSMVVRMITAGEQTGKIDNMLERISDFLDEEIETTLSGLT
                     SMIEPILIVFLGVVVGGMVICMFLPLFKLPEIVGGH"
     gene            complement(1010..1930)
                     /locus_tag="VIL39_04275"
     CDS             complement(1010..1930)
                     /locus_tag="VIL39_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEY5480672.1"
                     /translation="MNQLVEDFLQYLRHERGQAEHTQRTYAALLNKFVAWAAKQGISD
                     WQSVKLRHLMAFLQHERERALANQPKESARRLSSESLYLEIAALRALYRFAENEKLLP
                     ANVAENLSLPRRWKRLPKALTNQEIDKLLTPETPETPANLCDQAILELAYASGLRLAE
                     LRNARLENLHLDAGFINVVGKGNKERVVPVGSKAVAALEHYLSAGRPRLVTPRSTANV
                     FLTKRGTPFAPVTLWLRIKQRVRRAGIPRNVTPHMLRHSFATHLLDHGADLRVIQELL
                     GHASISTTEVYTHVAAQRLREVHRKCHPRP"
     gene            complement(1927..2820)
                     /locus_tag="VIL39_04280"
     CDS             complement(1927..2820)
                     /locus_tag="VIL39_04280"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5480673.1"
                     /translation="MATETLLQLDLPGLRKLRSGKVREVFDLGDRLLFVATDRISAFD
                     CIMPNGIPRKGEVLTQLSYFWFAQTRALQPNHVLSRPDDPLPPDLQPFAAQLARRSMI
                     VRKTEPLAIECVVRGYLAGSGWKEYRDQQTVCGIKLPPGLKESSELPGPIFTPATKAE
                     SGHDINIPFDEAARIVGADIAEQARAASIKIYRYASDYARRRGIIIADTKFEFGLLDD
                     KLILIDEILTPDSSRFWPADQYRPGQGQPSFDKQFVRDYLETLDWNKTPPAPALPPEV
                     VARTQAKYLEAYERLTGQKLE"
BASE COUNT          548 a          848 c          949 g          614 t
ORIGIN      
        1 agaccaccaa caaagtcatg cgcgatgtga ttcacgacgt ttgcacgcgg gttgaaagcg
       61 gcgacagttt ctccgaggca ctcgcaaagc acccgaaggc cttcagccgc ctatacattt
      121 gcatggtcgc cgccggcgaa caaggcgggt tgctggcgga gattctcgcc cggctggcga
      181 cctatctgga gactaccgcc cggctgcgca agaagatcaa atccgccatg atgtaccccg
      241 ccgtcgtgtc ggtggtggcg gtcctgatca cgactttcct gctgataaag gtggtgccgg
      301 tctttggcga aatcttcgcg agcttcggcg ccaagctgcc tgcgccgacg gcgtatctga
      361 tcatgctcag taatatcgtg aagcattacc tgattttcat tctgctcgct ggaggcgggg
      421 tggtgtatgg ctggttctac ttcatcaaga ccccggctgg ccggctcttc tgggattcgc
      481 gccgcattaa attgccgatt ttcggagcca tcgcccacaa gatttgcctc gcccggttta
      541 cccgcacgct ctcgtcgctg gtccgcagcg gcgtgccgat cctggaggtg ctgcaaattg
      601 tttcccaaac ggtcggcaac gtggtcatgg agaaggcgat caagaccgcc gcatcggata
      661 ttgagcgcgg cgagagcatt tccgccgccc tggccaagca tccgatattc ccctccatgg
      721 tcgtgcgcat gataaccgcc ggggaacaga ccggcaaaat tgacaatatg ctggagcgca
      781 tctcggactt cctggatgag gagatcgaaa caaccctttc gggtctgacc tcgatgatcg
      841 aacccattct aatcgttttc ctcggagtgg tggtgggcgg catggtcatc tgcatgttcc
      901 tgcccctctt caagttgccg gaaatcgtcg gtggccatta agtccccaat ccccaaagcc
      961 tactgatagc caaccgattg cggctccaaa gccaggttcg gccggaaagc taagggcgcg
     1021 gatggcactt gcggtggact tcgcgcaggc gctgggcggc cacgtgcgta tagacctccg
     1081 tcgtgctgat gctggcgtgg ccgagcaact cctgaatgac gcgcaagtcg gcgccgtggt
     1141 cgaggagatg agtggcgaaa ctatgccgca gcatgtgtgg ggtcacgttg cgggggatgc
     1201 cggcacgacg gacgcgttgt ttgatgcgca gccagagggt cacgggggcg aagggggtgc
     1261 cgcgtttggt gagaaagacg ttggcggtag atcggggggt cacgagccgg ggccgacccg
     1321 cactgaggta gtgttccaac gcggcgacgg ctttacttcc aacgggcacg acgcgctcct
     1381 tgttaccttt gccgacgaca ttgatgaagc cggcatcgag gtggaggttc tccagcctgg
     1441 cattgcgcaa ttccgctaag cgcaggcccg aggcgtaggc caactcgaga atggcctggt
     1501 cgcacaggtt tgcgggcgtc tcaggggttt cgggcgtgag cagtttgtcg atctcctgat
     1561 tggtgagggc tttgggcaaa cgtttccagc gccttggcag ggacaggttc tcggcgacat
     1621 tggccggcaa gagcttctcg ttctccgcaa agcgataaag ggcccgcaag gcggcaatct
     1681 ccagataaag gctttcgctg ctgaggcgac gcgcggactc tttgggctgg ttggccagcg
     1741 cccgctcgcg ctcgtgttgg aggaaagcca tcaagtggcg gagcttaacc gattgccagt
     1801 ctgagatacc ctgcttggcc gcccaggcga cgaacttgtt caacagcgcg gcgtaggttc
     1861 tctgcgtgtg ctccgcctgg ccgcgctcgt gacggaggta ttggaggaaa tcttcgacca
     1921 gttgattcat tccagcttct ggccggtcaa tcgttcatac gcctccagat acttcgcctg
     1981 cgtccgggcc accacctcgg gcggcaacgc gggcgcgggc ggtgtcttgt tccaatccag
     2041 ggtttccagg taatcgcgga cgaactgctt gtcgaagctc ggctgaccct ggccgggccg
     2101 atactggtca gccggccaga accgcgagga atcgggggtc aggatttcat caatcaggat
     2161 aagtttgtca tcgaggaggc cgaactcgaa tttggtatcc gcaatgatga tgccgcgccg
     2221 gcgcgcgtag tcgctggcgt aacggtagat ctttatggac gccgcccgcg cttgctcggc
     2281 gatgtccgcg cccacaatcc gcgcggcctc gtcaaatgga atgtttatat catggccgga
     2341 ctccgccttg gtggccggcg tgaagatggg cccgggcaat tccgaggatt ccttcaagcc
     2401 cggcgggagt ttgatgccgc agaccgtttg ctggtcgcga tattccttcc agccggagcc
     2461 ggcgaggtag ccgcgcacga cgcactcgat ggcgagcggc tcagtcttcc tgacgatcat
     2521 gctccggcgc gcgagttggg cggcaaaggg ctggaggtcg ggcggcaagg gatcatcggg
     2581 ccgcgagagc acgtggttgg gctggagggc cctggtctgg gcgaaccaga agtaagagag
     2641 ctgagtcagc acctcgcctt tgcgcggaat gccgttgggc atgatgcagt caaaggccga
     2701 aatccggtcc gtggcgacga agagcaggcg gtcccccaga tcaaacactt cccgcacttt
     2761 gccgcttcta agtttgcgca gaccgggcaa atcgagttgg agcaaggttt cagttgccat
     2821 gccggcaaat atgagacgag attcccccgg agcaaagagc aaagtgcgcc cctggcagcc
     2881 tctgagttcc cccggactcc ttcccccaca gcgttcattg ccggttctgg gtttggaact
     2941 tcttcgggtg gcagctctt
//
LOCUS       DATCGS010000213         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_17846, whole genome shotgun sequence.
ACCESSION   DATCGS010000213 DATCGS010000000
VERSION     DATCGS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17846"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1660
                     /locus_tag="VIL39_04285"
     CDS             47..1660
                     /locus_tag="VIL39_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5480674.1"
                     /translation="MKQAAAKELAADLVVIGGSVGGCAAALAAARNGLRVILTEETDW
                     IGGQLTSQAVPPDENAWIETCGGTRSYLNLRQEIRDYYLRHYPLTAEARAKPYLNPGN
                     GWVSRLCHEPRVALAVLQQLLAPYVGGQKILVLPRHKAMDALTGGDRVEAVRVRSLES
                     GKEVVLRAPYFVDATELGDLLPLTKTEYVTGAESQKETGEPHAPAEAAPHNMQSFTCC
                     FAMDYLRGEDHTIEKPREYAFWREFVPAVKPPWPGPLLSWPYSDAITSKPTKLETDPE
                     KGTGLWTYRRIADKSIFAAGAYPGDISLVNWPQNDYMLGNLCEVSAEEAARHVDRAKQ
                     LSLSLLYWLQTEAPRPDGGTGWKGMRLRPEIVGTEDGLAKYPYIRESRRIRAEFTVVE
                     QHVATEFRMKETGAKQEEVKAAEFKDSVGIGSYRIDLHPSTGGDNSIDISSLPYQIPL
                     GALLPQRVENLLPACKNLGVTHITNGCYRLHPVEWNIGEAVGALAAFCLTQKRLPREV
                     RKQGDLLKSFQTLLADQGVPLAWPKFTPR"
     gene            1664..1990
                     /gene="cutA"
                     /locus_tag="VIL39_04290"
     CDS             1664..1990
                     /gene="cutA"
                     /locus_tag="VIL39_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY5480675.1"
                     /translation="MKPKGKYAVVLVTAPDVKTARQLARAALEARLIACANLIPKIES
                     HYWWQGKSMIGAEVLLILKTTTARLAALEKLISARHPYDTPEFLVLPISRGNQRYLDW
                     VAESVE"
     gene            2023..>2328
                     /locus_tag="VIL39_04295"
     CDS             2023..>2328
                     /locus_tag="VIL39_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY5480676.1"
                     /translation="MDPLWPQNYDPLNNAVLSTALAALPIVVLLGSIALLRLRIHFSA
                     LLGLATALAIALGAYQMPAKAACAATLYGAAFGLFPIGWIILNVMFLYQITVQRGLFE
                     "
BASE COUNT          448 a          729 c          740 g          411 t
ORIGIN      
        1 cgttgccttc agccagaccc agaattcctc gactgcgcgt ggcagcctga agcaagccgc
       61 agcaaaggaa ctggctgccg accttgttgt catcggcggg agcgtcggcg gctgcgcggc
      121 ggcgttagcg gcggcgcgca atggcctgcg cgtcatcctg accgaggaga cggattggat
      181 tggcggccag ttaacgtcgc aagccgtgcc gccggatgag aatgcctgga tcgagacgtg
      241 tggcggcacg cggagttacc tgaacctgcg ccaggagatt cgcgattact atctgcgcca
      301 ttatcccctc accgccgagg cgcgggccaa gccctatctg aatcccggca acggctgggt
      361 ctcgcggctg tgccatgagc cgcgcgtcgc gctggccgtg ctgcagcagc tcctggcccc
      421 ttacgtgggc ggacagaaga tcctggtgct gccgcggcac aaggcgatgg acgctttgac
      481 cggcggcgac cgcgttgaag ccgtccgggt gcgcagtctt gaatcgggca aggaggttgt
      541 cctccgggcg ccttacttcg tggacgcaac cgagctgggc gacttgctgc ccctcacgaa
      601 gacggaatac gtcaccggcg ccgaatcgca gaaggagacg ggcgagccgc acgcgcccgc
      661 cgaagccgcg ccgcataata tgcagtcatt cacctgctgc ttcgcgatgg attacctccg
      721 cggtgaggac cacacgattg agaagccgcg cgagtatgcg ttctggcgcg agttcgtgcc
      781 cgctgtcaaa ccgccgtggc ccgggccctt gctctcgtgg ccctacagcg acgctattac
      841 cagcaagcct accaagctgg agaccgaccc ggaaaagggc accggcctgt ggacctatcg
      901 ccgcatcgcg gacaagtcga tcttcgccgc gggcgcgtat ccgggcgaca tctcgctggt
      961 gaactggccg cagaacgact acatgctcgg caacctgtgc gaggtcagcg ccgaggaagc
     1021 cgcgcgccac gtggaccgag ccaagcagct cagcctctcg ctgctctact ggctgcaaac
     1081 ggaagcgccc cgaccggacg gcggcaccgg ctggaaggga atgcgcctgc ggccggaaat
     1141 cgtcggcacc gaggacgggt tggccaagta tccttatatc cgcgagagcc ggcgcatccg
     1201 tgccgagttc acggttgtcg agcagcacgt agcgaccgag ttccgcatga aggagaccgg
     1261 agcaaagcaa gaagaagtga aggccgcgga gttcaaggat tcggtgggca tcggcagtta
     1321 ccggattgat ctgcatccca gcaccggcgg ggacaactcc attgacatca gttccctgcc
     1381 ctaccaaatc ccgctcgggg cgttgctgcc gcaacgggtc gagaacctgc tgccggcctg
     1441 caaaaacctc ggggtgacgc acatcactaa cggctgttac cggttacatc ccgtggagtg
     1501 gaacatcggc gaagcggtgg gcgcgctggc ggcattttgt ctgacgcaga aacgcctgcc
     1561 ccgtgaggtg cgcaaacagg gcgacctgct gaagagcttt cagaccctgc tcgccgatca
     1621 gggcgtgccg ctcgcgtggc caaagttcac gccgaggtaa gatatgaagc ctaaaggaaa
     1681 atatgcagtg gttctggtca ccgcgccgga cgtgaaaacc gcgcgccaac tggcccgggc
     1741 ggcgctggag gcccgactga ttgcgtgcgc caacttgatt cccaaaatcg agtcgcatta
     1801 ctggtggcag ggcaagagta tgatcggggc cgaggtgctg ctgatcttaa aaacaaccac
     1861 ggcccgcctg gctgcgttgg agaagctgat tagcgccagg cacccgtacg acacgccaga
     1921 gttcctcgtg ctgcccatca gtcgcgggaa ccagcgctac ctggactggg tggccgaatc
     1981 cgtggaatga accctgtgac gccgctaatc tgaatccaga caatggaccc gctctggccg
     2041 cagaactacg atccgctcaa taatgcggtc ctttccacag ccctggctgc gctgcccatt
     2101 gtggttctgc tcggctcgat tgccctgcta agactccgaa tccatttctc ggccttgctg
     2161 gggctggcca ctgccctggc cattgcgctg ggggcatatc agatgccggc caaagccgcc
     2221 tgtgcggcca cgctttacgg cgcggcgttt ggattgtttc ccattggctg gatcatactg
     2281 aacgtcatgt ttctctacca aatcacggtg caacgcggcc tgttcgaa
//
LOCUS       DATCGS010000214         8013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_118861, whole genome shotgun sequence.
ACCESSION   DATCGS010000214 DATCGS010000000
VERSION     DATCGS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118861"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..215)
                     /locus_tag="VIL39_04300"
     CDS             complement(39..215)
                     /locus_tag="VIL39_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480677.1"
                     /translation="METGQVWRMEDSQLHVDLVGKHLVHYKLIKHEAKRTPMSLSNKK
                     AVEKYLKDNKAVLV"
     gene            354..902
                     /locus_tag="VIL39_04305"
     CDS             354..902
                     /locus_tag="VIL39_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEY5480678.1"
                     /translation="MQSVFIGPIFVVRDNIDTDQIIPAQYLNLVPTIPEEYEKLGAYA
                     FAGLPESAYPGRYVKEGQLDSDYPIVVAGRNFGCGSSREHAPIALGSAGCKVVLAESF
                     ARIFFRNSVATGELYPCESADRLCDALKTGDVVTVDLDAGTVTAKSTGKTYEFKPLGD
                     VRSVVDAGGLFNYARKTGMIPK"
     gene            complement(1031..3367)
                     /gene="pnp"
                     /locus_tag="VIL39_04310"
     CDS             complement(1031..3367)
                     /gene="pnp"
                     /locus_tag="VIL39_04310"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5480679.1"
                     /translation="MSDKVIAPVGDKSLIIETGKIAKQADGAVTVQLGETIVIVAAVA
                     ATKAKPGQEFFPLTVDYREKAAAAGKFPGGYFKREGRPTEKEILTCRLVDRPIRPLFP
                     KGMYNEVQVQCIVLSADGENDPDMLALIGASTALMVSDIPWAGPIGAVRVGRLGGQFV
                     ANPTHTQMLESDLDLVYVGNESDMVMYEGSAKEITEADFNAALKFGQEAIQPAIAAQK
                     ELVARVGKPKRQITLNVVPDEILKEAKALAGDRIVAALLTPGKLDREGAVAAITEEIG
                     KKLVEKFGAEKVTEFVLKDAFYYIQKESVRGLIMNQGKRLDGRDFETVRPIVSEVGIL
                     PRAHGSALFQRGETQAVTLCTLGTGEDAQEFDSYTGGASEKKFILHYNFPNFSVGETG
                     RISGPGRREIGHGALAERSLEPMVPTATYPYSVRITSEIMESNGSSSMASVCGGCLAL
                     MDAGVPMTRPVGGISIGLCTEHDAQDKITSYKLLTDIIGWEDAFCDMDCKIAGTEKGI
                     TGFQLDLKLRGIPLSLMTETVEKARIARLHVLAEMAKTLATPRSEISKYAPRIQTVKI
                     NPEKIGLLIGPGGKNIKKLVEESGCEINIEDDGTVNIYSVSAEGMKIAVDAIAGMTAE
                     AEINKVYRGRVVTIKEFGAFVEFLPGKDGLVHISELANFRVKQVEDIVKMGDEIWVKC
                     LGVDEKGRVRLSRRAAMAERDQQMGGAPVEGAPAGEGGQPVEGGDQGQPREGGEQPYG
                     DRGEHRGEPRGDQGDPRGGDRGPRRDGGGRGGERRGRR"
     gene            complement(3576..3833)
                     /gene="rpsO"
                     /locus_tag="VIL39_04315"
     CDS             complement(3576..3833)
                     /gene="rpsO"
                     /locus_tag="VIL39_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5480680.1"
                     /translation="MEAKAKTIDGFKLHERDTGSADVQIALLTQRINHLTEHLQTNKK
                     DHGSRRGLLLMVGQRRRLLDYLQTIDVNRYVAITKKLKLRK"
     gene            complement(4071..4670)
                     /locus_tag="VIL39_04320"
     CDS             complement(4071..4670)
                     /locus_tag="VIL39_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY5480681.1"
                     /translation="MPINFFDAVVIVVLLVGIAQGRKRGMSEELLNLIQWLVILFGCA
                     AIYEPTGEMIRHFTGMFSRLSCYLLAYVGVGLLVVLVFAGIKRTLGGKLIGSDIFGRS
                     EYYLGMASGLVRSSCILLAVLALLHARYFNPTEVRAMEKLQEDVYGSNFFPTLQSLQA
                     TVFDKSLTGSWIKQNLSFLLIKPTEPEKMEIHQRDAVIP"
     gene            complement(4845..6356)
                     /locus_tag="VIL39_04325"
     CDS             complement(4845..6356)
                     /locus_tag="VIL39_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480682.1"
                     /translation="MLLLALLAGCTPPGPRALFDGQRLIEQGKYPQAIEKLRAATLLL
                     GGTNALAWHYLGLACQQAGEAAEAERAYRKALELDHDLSEAHYNLGCLWLAQNRIEAA
                     RGEFTACTLRRPNMVDGFLKLGAVQLRAREPSAAEKSFSEALRLSPHNPEALNGLGLA
                     RLQRGRATEAAQCFDDALKQQPDYRPALLNLAIVSHQYLKDRQLALEKYREYLALKPP
                     PPNAESLMATVRQLEQELNPPSRRAVTNELAQPIPKANTSKPAVTNVTRITNSPKAEP
                     PPGPTKPTFLHNGDKTPVRPTPLGAEAAPPEGESAKRAATGGEPAVSLPSPLPNRTAT
                     RYAYRSPAAPAPGNRADAERACAQGVQAYEAHRLPEAMQAYRLAAQLDPSLFEAPYNL
                     GLAATQAGNFPAALTAYESALAVQPRSLDARYNFALVLKQANYIADAVNELERVVVTY
                     PNETRAHLALGNLYAQQLQQPAKARQHYSKVLEVEPRHPQARDIRYWLAANPP"
     gene            6564..7664
                     /locus_tag="VIL39_04330"
     CDS             6564..7664
                     /locus_tag="VIL39_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480683.1"
                     /translation="MLVRFASRFAAGLTALRPNTPQPLAALAVCLLSLSLLPAQAADA
                     SSSGSRAPRAYVTVTQDPEATDAYRPRPEKVRLMVNRAITNLTHKPTVPDAWRSLVST
                     QDVVGIKVFSAPGPNSGTRPAVVAAVVEGLLAAGLPPKHIIVWDRQITDLRLAGFAEL
                     SDRYGIRIAGSAQAGWDEKTFYNPDSPILGNLVWGDLEFGKKGDGVGRKSFVSTLVSR
                     ELTKIINITPLLNHNEAGVSGHLYSLATGSVDNLIRFDSSPETLATAIPEIYSLPALS
                     DHVVLSITDALICQYEGGERGLLHYSATLNQLRFSRDPVALDVLSIKELDHQRQMTSA
                     PSIKPNLKLYRNAALLQLGVYDLKEIQVDTLP"
     gene            complement(7671..>8013)
                     /locus_tag="VIL39_04335"
     CDS             complement(7671..>8013)
                     /locus_tag="VIL39_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480684.1"
                     /translation="QAPPSLHGSIMRAVRAAEHPVAEARRGLTFLRWLPVPVVALLAL
                     LVVWHVARGPVRVPMQDTQSLTVATTALEMGGQMARAMPSAVVSPLAEELEKVNRDLD
                     STAQFILASLP"
BASE COUNT         1515 a         2551 c         2335 g         1612 t
ORIGIN      
        1 cacctgactg ttgctggagt ggtccgactt ccctatcctt agaccagaac ggccttgttg
       61 tccttgagat acttctccac ggcctttttg ttggacagcg acatgggagt gcgcttggcc
      121 tcgtgtttga tgagtttgta atgcaccagg tgcttgccca ccaggtcaac gtggagttgc
      181 gaatcctcca tgcgccagac ctgtccagtt tccaagggct tgagagggat tcgacttttt
      241 tgcttcatca gtatgtaaga acaagggtgt cagagcggaa cagaaagagc aatgcaaatc
      301 ggagtgctgc aaatctggtt gaatattggc gtagttggcg ccactctccg ggcatgcaat
      361 ctgttttcat aggaccgatc tttgtggtgc gggataatat tgataccgac cagattatcc
      421 ccgcgcaata cttgaatctc gtgccgacga ttcccgagga gtacgagaag ctcggcgcct
      481 acgccttcgc cggcctgccg gaatccgctt acccgggacg ctacgtgaag gaaggccagc
      541 tcgacagcga ctacccgatc gtggtcgccg gacgcaattt cggctgcggc agttcccgcg
      601 agcatgcgcc cattgcctta ggctcagcag gctgcaaggt cgtgctggcc gagagtttcg
      661 cgcgcatctt cttccgcaac tcggtcgcaa cgggcgagct ttatccctgt gagtcggcgg
      721 accgcctgtg cgatgcgttg aagacaggcg acgtggtcac ggtggacttg gacgcgggca
      781 cagtaaccgc caagagcacg ggcaagacct atgagttcaa gccccttggc gatgtgcgat
      841 cggtggtgga tgcgggcggg ctgttcaatt acgcgcgcaa gacggggatg ataccgaagt
      901 aacacgcgcc ccaacgggtt ccctctcacc aatccgggcc aagtcgggcg ataccgaatc
      961 aactctgaag cccgaacgac aaaaaaggcg agccgttgcc ggctcgccta tgaagtgatt
     1021 ctgggccgaa ctaccttctc ccgcggcgct caccgccccg gcccccgccg tctcggcgtg
     1081 gcccgcgatc gcccccgcga ggatcaccct gatcgccacg cggctcgccg cggtgctcac
     1141 cccggtcacc gtagggctgt tccccgccct cgcgaggctg gccttggtcg ccgccttcga
     1201 cgggttggcc accttcgccg gctggggcgc cttcgaccgg tgcgccgccc atttgctggt
     1261 cgcgctcggc catagcagcc cggcgagaga gccgcacgcg gcccttttca tccacgccca
     1321 gacatttgac ccagatctcg tcgcccatct tgacgatgtc ctcgacctgt ttaactcgga
     1381 agttggccag ctcgctgatg tgaaccaggc cgtccttgcc gggcaggaac tcgacgaacg
     1441 cgccaaactc cttgatggtg acgactcgac cgcgatacac cttgttgatc tcggcttcgg
     1501 cggtcatgcc cgcgatggcg tccacggcga tcttcatccc ctcggcggag accgagtaga
     1561 tgttgaccgt accgtcgtct tcgatgttga tttcgcagcc ggattcctcg acgagtttct
     1621 tgatgttctt gccgccgggg ccgatcagca gaccaatctt ctcggggttg atcttcaccg
     1681 tctggatgcg tggggcatac ttgctgattt ccgaacgcgg ggtggccagc gtcttggcca
     1741 tctcggccag cacgtgcagc cgcgcgatgc gtgccttctc gacggtctcg gtcatcagac
     1801 tgagcgggat gccgcgcagc ttaaggtcga gttggaagcc ggtgatgcct ttctcggtcc
     1861 cggcaatctt gcagtccatg tcgcagaacg cgtcttccca accgatgatg tcggtgagca
     1921 gcttatagct cgtgatcttg tcctgagcgt cgtgctcggt gcataggccg atgctgatgc
     1981 cccccaccgg gcgggtcatc ggcacgcccg cgtccatcaa cgccaggcag ccgccgcaga
     2041 cggaggccat cgaggaggag ccgttggact ccatgatctc gctggtgatg cgaacggaat
     2101 aggggtaggt ggcggtcggc accatcggtt caagcgagcg ctcagctaat gcgccgtggc
     2161 caatctcgcg ccgacccgga ccgctgatgc ggccggtttc gccgacggag aagttcggga
     2221 agttgtagtg caggatgaat ttcttctcgc tggcgccgcc ggtgtaggag tcgaactcct
     2281 gcgcgtcctc gccggtgccc agggtgcaaa gcgtgacagc ctgcgtctcc ccgcgctgga
     2341 acagcgccga gccgtgcgcc cgcggcagga tgcccacctc gctgacaatg ggccggacgg
     2401 tttcgaaatc gcgaccgtcg agccgcttgc cttggttcat gatgaggccg cggacagatt
     2461 ccttctggat gtagtagaag gcatccttga gcacgaactc ggtgactttc tccgcgccga
     2521 acttctcgac gagcttcttg ccgatttctt ccgtgatggc cgcgacggcg ccttcgcgat
     2581 cgagcttgcc cggcgtgagg agggccgcga cgatgcggtc gcccgccagc gccttggctt
     2641 ccttgaggat ctcgtccgga acgacgttga gcgtgatttg gcgtttcggc ttgccaaccc
     2701 gggcgaccaa ttccttctgc gcggcaatcg ccggctgaat cgcttcctga ccgaacttaa
     2761 gcgcggcgtt gaagtccgct tcggtgattt ccttggccga gccttcatac atcaccatgt
     2821 ccgactcgtt gccgacatag accaggtcca aatcgctttc gagcatctgg gtatgggtgg
     2881 ggttggcgac gaactggccg ccgaggcgcc cgacgcggac cgccccgata ggacccgccc
     2941 agggaatgtc gctgaccatc aacgccgtcg aggcgccgat gagcgcgagc atatccgggt
     3001 cgttctcgcc atcggcgctc agcacgatgc attggacctg gacttcgtta tacatgccct
     3061 tgggaaagag cggccggatc ggccggtcca cgagccggca ggtgaggatt tccttctcgg
     3121 tcgggcggcc ttcgcgtttg aagtagccgc cagggaactt gccggccgca gccgcctttt
     3181 cccggtaatc caccgtcaac ggaaagaact cctgacccgg tttggccttg gtggcggcga
     3241 cggccgccac gatgacaatg gtttcgccca gttggacagt tacggcgccg tcggcctgtt
     3301 tggcgatttt gccggtttca atgatgagtg acttgtcccc aacgggggca atgactttat
     3361 ctgacatatt atgtaacccc tccattgcca ccgaggaact tcagtgaatc ccgaaaccat
     3421 tcgggactga acgaaattac ccagggcaac ggagctttct tttacgcgat tgttaaggca
     3481 gaaaaccggg ccgttcgcgt ttaccggtcc gattaaatcg tgagcctccc ggcgacggac
     3541 gtgtccgtcg cgacactcag gagggcaggc cgcggctact ttcgcagctt gagcttcttg
     3601 gtaatcgcca cgtagcggtt gacgtcgatg gtctgaagat agtccagcaa acgccggcgt
     3661 tgaccgacca tcagcagcag cccgcggcgg gaaccatggt ccttcttatt cgtctgcaga
     3721 tgctccgtca ggtggttgat gcgttgtgtg agcagcgcaa tttgcacatc cgccgaacct
     3781 gtatcccgct cgtgcagctt gaaaccatca atcgtttttg ctttcgcttc catactttct
     3841 aatacgtcca atcaatccaa acaatccaaa caatccttca tttaattcaa tatagacacc
     3901 gaatagtagt ttctccgctc gggcgtgtaa agcgcttttg gagcatcgtt gtcagagaat
     3961 gaaattaggg cttgggacga ggtgccaccg accccatgca tctgccacgg agacgcggaa
     4021 cagaccggcc atggccacgc cgacacaagc atccaggatc cgattagcac tcaggggatg
     4081 accgcgtccc tttgatggat ctccatcttc tccggctccg tcggcttgat caataggaag
     4141 ctgaggttct gtttgatcca cgagcccgtg agcgacttat cgaaaaccgt ggcctgcagc
     4201 gactgcagcg tcgggaagaa gttgctccca tagacatcct cctgcaactt ctccattgcc
     4261 cggacttcag tggggttaaa ataacgggca tgaagcaacg ccagcacggc cagcaagatg
     4321 cagctagacc gcaccagtcc cgacgccatg cccaaataat attccgagcg tccaaaaatg
     4381 tcgctgccta tcagtttgcc acccaaagtg cgcttgattc ccgcaaatac aagcactacc
     4441 agcaggccca cgcccacata agccagcaaa taacaggaca gccggctgaa catgccagta
     4501 aagtgcctga tcatttcccc agtcggctcg taaatggccg cgcagccgaa gaggatcacc
     4561 aaccattgaa tcaagttcag cagttcctcc gacatcccgc gcttacggcc ctgggcaatg
     4621 cccacaagca gcacgacgat tactaccgca tcaaaaaagt taattggcaa ctgctcaagg
     4681 ctcattcttg tgttctgtca ttcgcatccc ggcggagatg cgggttttat ggacagtgtt
     4741 agccagtttt gtgccatcga agcccttgca atgcagatcg ccgacccgac ccaaggccgg
     4801 gaaaaggccg ttaccgtgcc tcctattggg tcccgcaatc gcggctaagg cggattagcc
     4861 gccaaccagt aacgaatatc gcgcgcctgc ggatgacgcg gctccacttc cagcaccttc
     4921 gaataatgct ggcgggcttt ggccggctgt tgcaactgct gcgcgtagag attcccgagc
     4981 gccaggtggg cgcgggtttc gttcggatag gtgaccacga cccgttccag ctcattgacg
     5041 gcgtcggcaa tgtagttggc ttgcttgagg accaacgcaa aattgtagcg ggcatcgagc
     5101 gaccgcggct ggaccgccag agcgctttca taggccgtca gcgcagccgg gaaattgccg
     5161 gcttgcgtgg ccgccaggcc caaattgtaa ggcgcctcaa agaggctcgg atccagttgc
     5221 gccgccagac gataggcctg catcgcttcc ggcagccgat gcgcttcgta cgcctgcacc
     5281 ccttgcgcgc acgcgcgctc cgcgtcagcg cgattcccgg gcgcgggcgc ggcgggagac
     5341 ctataggcgt agcgagtcgc cgttctattg ggaagaggcg acgggaggct gaccgcgggc
     5401 tctcctccgg tggccgctct cttcgccgat tcaccttccg ggggagccgc ttcagcaccc
     5461 agcggtgttg gacgcaccgg cgtcttgtca ccgttgtgaa ggaaagtcgg tttcgtcgga
     5521 ccaggcggcg gttccgcttt gggcgaattg gtgattcggg tgacattcgt cacagccggt
     5581 ttcgacgtat tcgccttggg gatgggttga gccagttcgt tcgtcaccgc gcgccgcgat
     5641 ggcggattga gttcctgttc gagctgccga acggtcgcca tcaggctctc cgcgttcggc
     5701 ggcggcggct ttaacgcaag gtactcccga tacttctcca gcgccaactg acgatctttc
     5761 agatactggt gcgatacaat cgccaggttc agcagggcgg gacggtaatc cggctgctgc
     5821 ttcagcgcgt cgtcaaaaca ctgcgccgct tcggtggccc ggccgcgttg aagccgcgcc
     5881 aggcccagcc cattcaatgc ctccgggttg tgcggactca gccgcaacgc ctcgctgaag
     5941 ctcttctccg ccgcgctcgg ctcgcgggcg cgcaattgca cggcgcctag tttgaggaaa
     6001 ccgtcaacca tgtttggccg gcgcaaggtg caggccgtga actcgcccct ggccgcctcg
     6061 atccgatttt gcgccagcca caggcagccg agattgtagt gcgcctcgct taaatcgtgg
     6121 tccagctcca gcgctttccg gtaagccctc tcggcctcag ccgcctcgcc ggcctgctgg
     6181 caagccagac ccaggtaatg ccatgccagc gcgttcgtcc ccccgagcag caaggtggcc
     6241 gctctcagct tctcgattgc ctgcgggtac tttccctgct ctatcagccg ttggccatcg
     6301 aacaaggcgc gcgggcccgg cggagtgcag ccagccagca gcgcgagcaa caacaccagc
     6361 ctccacacgt tagatggaat ccatgtgtga ttctctcgtc cgccagcgag ctcaagccca
     6421 ggccggtttt tgatggtcaa catcggtgat ggtggtaaca tccgcgccct gaagtgtcaa
     6481 gaaacgcacc atttagcaat gattcggttg accccgtagc cgagggagca cggggcccac
     6541 gccgtttcac agacgctggt tccatgctgg tgcggtttgc ttcgcggttc gccgccggcc
     6601 tgaccgcact gagaccaaac actccccagc cactcgccgc gctggccgtc tgcctcctca
     6661 gcctctcgct cctgccggcg caggccgcgg acgcttcctc ctccggcagc cgtgccccgc
     6721 gcgcctatgt tactgtcaca caggacccgg aggctaccga cgcctaccga ccgcgcccgg
     6781 agaaagtccg gctcatggtc aaccgcgcga tcacgaacct gacccacaag cccaccgtgc
     6841 cggacgcctg gcgcagcctg gtttcgaccc aagacgtcgt cggcattaaa gtcttctcgg
     6901 cgcccggccc gaacagcggc acgcgtccgg cggtggtggc ggcggtcgtc gaaggtctgc
     6961 tcgctgcggg tttgccgccc aaacacatca tcgtatggga ccgacaaatc accgacctgc
     7021 ggctcgcggg ttttgctgaa ctttccgacc gctacggcat tcgtatcgct ggcagtgcgc
     7081 aagccggctg ggacgagaaa accttctaca accccgatag ccccattctc ggcaacctcg
     7141 tctggggaga cctggaattc ggaaagaagg gcgatggcgt cggacgaaaa tcctttgtct
     7201 ccaccctcgt cagccgcgag ttgacgaaga tcatcaatat caccccactg ctaaaccaca
     7261 atgaagccgg tgtttcgggc cacctttaca gcctggcgac cggcagcgtt gataacctca
     7321 tccgctttga ctccagcccg gaaacgttgg ccacggctat ccccgagatc tacagcctgc
     7381 cggcgctcag cgaccacgtt gtgctcagca tcaccgatgc gctgatttgc cagtacgaag
     7441 gcggcgagcg cgggctgctc cactactcag ccaccttgaa ccagcttcgc ttcagccgtg
     7501 atcccgtcgc gctcgatgtg ctttcaatca aggaactcga ccaccagcgc cagatgacct
     7561 ccgcacccag catcaaaccg aacctcaagc tgtacaggaa cgccgccctg ctccagcttg
     7621 gcgtgtacga cttgaaggaa atccaagtgg acaccctccc ataacgagcc tcaaggcagg
     7681 cttgccagga tgaactgcgc cgtgctgtcc aggtcacggt tgaccttctc caattcctca
     7741 gccagcgggc tgacgactgc agaaggcatg gcccgcgcca tttggccgcc catctctaaa
     7801 gcagtggtgg cgaccgtcaa cgactgagtg tcttgcatcg gcacccgcac cggaccgcgc
     7861 gccacatgcc agaccaccag cagagccagc aacgcgacaa caggcacagg aagccagcgc
     7921 agaaaggtca gcccacgtcg cgcttccgcc accgggtgct cggctgcccg caccgcccgc
     7981 ataatcgagc catgcaacga tggcggggcc tga
//
LOCUS       DATCGS010000215         6013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_168736, whole genome shotgun sequence.
ACCESSION   DATCGS010000215 DATCGS010000000
VERSION     DATCGS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168736"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..>6013
                     /locus_tag="VIL39_04340"
     CDS             476..>6013
                     /locus_tag="VIL39_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEY5480685.1"
                     /translation="MKSTLKMLGATLLGVAWSGALFAGHTNFDFSIDPRASGNDPTGN
                     PLFYFAGAGTPSYNNPASPDYFWRSANGNPGGYLALTDNQNNESMVVVLPQLDFYTNA
                     SAVVTPSPIRNFRIDVDVRIGNPTNVDSAPADGFSISFARSTDPALLNAINGNLYGFA
                     GGASLAEAQAGPNPTGGAYNTPENGVRQGVSIVFDAHQGNYLPDTAPSDGASRFPLPG
                     HSNDREGITLRVDDKTLIQGNFGTANRNGACSNLFNATATNACAISGFACTNINTLQT
                     GPFGGALNTVSNALLCWQHLSITLSNKVLTVVWKGRTNMTTTVTNWLGGTGRFMLAGR
                     TGGDNQIQHFDNLVIDTTPFTTSFLNPTVGNNYGFMFNVQDFGDSLVTNITQVVLDGS
                     IDVTSQIVLSNSPPSTFGIYTQAMPFAAGSTHTVAVSWQTSLGELLSATNQFMVAAYS
                     TIPDSYLVPLSAIDTNQPGFLVHSHQSYQHGGASARYVEEQLIGLHGANIADQSQATH
                     GGLFAWTGAIDFMTPGGAAAAQGMFFANNDFVNFGIGAVTGGGNNGSTTEPDFAVEFF
                     TYVYFPTNGLYTFVVAGDDSHQLRFSRNPMDKFGVIGTTLDVNQMPISSLPPAAGGEE
                     VTLYVSHAACLPIRILVGNVASDGALEVYTANADGTFSALNDINDPNALLCYQVSSVY
                     GPYVKSANPVWDAQNVVFYQPIVMQLGDGTGNLAIDTNTVVLKSTTMADLSTMVTQSI
                     TLATSAGITTLAQQKGTYPQWTNWPKAGTITNTLSFNDLNGTNYTYTWTFSVLNTAAN
                     IATNALPIPATLGVDSSLVDLARPGWRIKSWQSAQESPNVPAWTLEQLEGLHGPNLAD
                     QSGTNGPGFFVRNDLLDLRIGTGTAPAGSSPGEWGYDLGYDATPGGSLSQFGIGALAS
                     TGGNGNTLAPTFRFAGDNRMETAALDIGAWLVFPAAGTYIMQVNSDDGFIAGFPQGSP
                     FGGLGLVGTYANATLFSGGRGLAGTPTGADTGGTLVSINIPQAGAYPFRLVYYNGGTA
                     GGLELSVYQALPDGSIAKVPVNDPAQPYSIKAYQTLTSGEAAAPYLKYMNPVSTAVDI
                     LPWQPVVADLADGAGTKTVVPTSIQLKVDGVVAAIIKTNPTPGITHIIEQVGMARSAT
                     AHTNVLTYTDNSGASYTNTWAFTVLNNLPFGITTLPLASMRPTNQVDHSQPGFRVKAY
                     QTTAASPPNVTFAEEAFAGLHGANIATAVATPPEGYFVWNNEMDFADDIAANAGNGEY
                     RYNYFLSQYFGYVLQGGGLAVNYNEAIFAGWMEFPTAGLYAMTVNRDDGFRLTCPYGS
                     NPFEEAGQVVGVFDGSGGNGGAAYPPTAGLRFVFNVPAAGAYPLRLIWYNGTGGASLE
                     WSVWQYLPDGSMARLLVNDPNTLGALKVYQTLTNGSAPYVAFINPYPGTALGQQANVF
                     WQSTPIPGSTFVPAVSEADLTVALQDGATTVLTNTITLTFNGVVQPITIGTSNGLTTI
                     TRAATNGFWPSGGLGPLVLSFTDSASNTYSYPVEILATPFWGTLTNAVTAQVDTNKPG
                     FKMRIYQLDPKATAGTTVIPTRVHVAEQVLSGVWGTNAVKPTAYTNNGYVNLQGTGPT
                     AGTINFNLLGAAQIGDFQSGGGFADQIWPGVPGFITATVNSDTNNDFAAEILAYVEFP
                     TNGTYFLAVSSDDGFRLWNGWTPPVNKGALVVNSPVALAGIKATVINGSTANTPFISL
                     PLTNAINGDLVLAQGIGFGSTTNGEGCVITNPGQLAGKIAVMYRSAFCGYAQQVANAQ
                     AAGALAVVLIQNRPATEGPFPQEPAISPWAAIPAIQIEQADGNALVAALNGGTNVNVT
                     LTPL"
BASE COUNT         1199 a         2063 c         1636 g         1115 t
ORIGIN      
        1 ttttccattc cccgccaagt gccgatgggc cgtacacata cgaagcctgc gaggaggacc
       61 cgatgcccga ctacgaaaac gcgctcacgg attgaggatc ccagagacca agctggaagg
      121 cgggtccgtc gccgggcgcg gctctctctc accggtttgg ccagcgcagg gtcggcctcc
      181 tcttccgcct tcgccactcc cggtccccat tgcccccata atcccgctgc ccccgcaagc
      241 tagcgcccca ggggcgcggc caaaaccatt gaaaaaatgg caagaactgc cccttgacat
      301 tctggccgtc ctttggcaca ctcggcccgt aatggcagag acccgaaacc aattctctct
      361 cactcgcgta gcgctgagta catgtgggcg cgcgaaaagc acattcctga catcagtatc
      421 agttccatga accaagaccc ataaaagaag cactacggag aacaccccca atacaatgaa
      481 aagcacactc aaaatgcttg gtgcgacgct gctcggcgtc gcttggtccg gagccctgtt
      541 cgcggggcac acgaacttcg atttttccat agatcccagg gcgtctggga acgatccaac
      601 gggcaacccg ctcttttact tcgcgggcgc gggtactcct agttataaca acccggcctc
      661 cccggattac ttctggcgct cggccaacgg caatccaggt gggtacctcg cgctcacgga
      721 caaccagaac aacgaaagca tggtggtggt gctgccgcaa ctggacttct acaccaacgc
      781 ctcggcggtc gttactccca gccccatccg caatttcaga attgatgttg atgtacggat
      841 aggcaacccc acgaacgttg actccgcccc cgccgacggc ttcagcatca gcttcgctcg
      901 ctcgaccgat cccgcgctcc tcaatgcgat caatggcaac ctttatggtt ttgccggcgg
      961 cgccagcctc gccgaggcgc aagcgggccc caaccccacg ggcggcgcct acaacacccc
     1021 cgagaatggt gtcagacagg gtgtctccat cgtgttcgat gcgcaccagg gcaattacct
     1081 gccggatacg gcaccgagcg atggcgcgtc gcgtttccct ctcccgggcc actccaatga
     1141 ccgcgagggc atcaccttgc gagtggacga taaaactctg atccaaggca acttcgggac
     1201 agcgaaccgg aatggcgctt gcagcaacct ttttaacgcc accgccacca acgcctgcgc
     1261 catcagcggc ttcgcctgca cgaacatcaa tacgctccag accggtccct tcggcggggc
     1321 cctaaacacg gtcagcaacg cattgctatg ctggcagcac ctctcgatta ccctgagcaa
     1381 caaggtcctg acggtcgtct ggaagggccg caccaacatg accaccaccg tcaccaactg
     1441 gctcggcggc accgggcgtt tcatgctggc cggccgaacc gggggcgaca accaaatcca
     1501 gcacttcgac aatctggtca tcgataccac cccctttacc acttccttcc tcaacccgac
     1561 cgtcggcaac aactacggct ttatgttcaa tgtgcaggac ttcggcgaca gcctggtaac
     1621 caacatcact caggtggtcc tggatggcag catcgacgtc acctcccaaa tcgtgctcag
     1681 caattctccc ccctccacct tcggcatcta cacccaggct atgcccttcg cggccggctc
     1741 gacgcacacc gtggcagtga gctggcagac cagcctgggc gaactgctca gcgccaccaa
     1801 ccagtttatg gtggcggcct actcaacgat ccccgacagc tacctggtgc ccttaagcgc
     1861 catcgacacc aaccaacccg gcttcctggt acactcgcat cagagctacc agcatggcgg
     1921 tgcatccgcc cgctatgtcg aagagcagct tatcggcctc catggcgcaa acatcgccga
     1981 ccagtcgcaa gctacccacg gcggcctctt cgcctggacc ggggcgatcg atttcatgac
     2041 gcctggcggc gccgcggccg cccaaggcat gttcttcgcc aacaatgact ttgttaattt
     2101 cggcatcggc gccgtgacgg gcggcggcaa caacggctcc accaccgagc ccgacttcgc
     2161 ggttgagttt tttacctacg tctatttccc gaccaacggc ttgtacacgt ttgtggtcgc
     2221 gggcgacgac agccatcaat tgcgctttag ccgaaatccg atggacaagt ttggcgtgat
     2281 tggcacgaca ctggatgtca atcagatgcc aatctccagc ctgcccccag ccgctggtgg
     2341 ggaggaagtc acgctgtatg tcagccatgc cgcgtgtttg cctatcagga ttctggtggg
     2401 taatgtcgcc agcgacggcg cgcttgaggt ctataccgcg aatgcggacg ggaccttttc
     2461 cgcgctcaac gacatcaacg accccaatgc gctgctgtgc taccaggtca gctccgtcta
     2521 tgggccttac gtgaaatcgg ccaacccggt ctgggacgca cagaacgtgg tgttttacca
     2581 gccgattgtg atgcaactcg gcgacgggac cggcaacctg gccatcgaca ccaatacggt
     2641 cgtgctcaaa tccacgacta tggccgatct cagcacgatg gtaacccaga gtattaccct
     2701 cgctacctca gcgggaatca ccacgctggc gcagcaaaag ggaacctatc cgcagtggac
     2761 caactggccc aaggccggca cgatcaccaa taccctgagt ttcaatgacc tgaacggcac
     2821 caactacact tatacctgga ccttcagcgt cctcaatacc gccgccaaca tcgccaccaa
     2881 tgcgctgccg attcccgcca ccctcggggt cgattcctcc ctggtggatc tcgcccggcc
     2941 cggctggcgc attaaatcct ggcagagcgc gcaggagtcg cccaatgtgc cggcgtggac
     3001 gctcgagcaa ttggaaggac tgcacgggcc gaacctggcg gaccagagcg ggaccaatgg
     3061 ccccggcttc tttgtccgga atgatctgct cgatttgcgc atcggcaccg gcacggcgcc
     3121 cgcaggcagc tcgcccgggg aatggggtta tgatcttggc tacgatgcca cgcccggcgg
     3181 cagtttaagc cagtttggca ttggtgcgct agcgagcacc ggcggtaacg gcaatacact
     3241 ggctccgacc ttccggtttg ccggggacaa tcggatggag accgccgcgt tggatatcgg
     3301 cgcctggctg gtcttcccgg ccgctggaac ttacatcatg caggtgaaca gcgacgacgg
     3361 tttcattgcc ggtttcccgc agggcagtcc cttcggaggg ttgggcttgg tcggaacata
     3421 cgccaacgcc accttattca gcggcggccg cggtttggca gggaccccca ctggggccga
     3481 caccggcggc acgcttgtca gcatcaacat tccgcaggcg ggcgcctatc ccttccgcct
     3541 ggtatactac aacggcggaa ccgccggcgg ccttgagtta tccgtttacc aggctttgcc
     3601 ggacggctcg attgccaagg ttcccgtcaa tgacccggct cagccttatt cgatcaaggc
     3661 ctaccagacc ttgaccagcg gcgaggccgc ggcgccctat ctcaagtaca tgaacccggt
     3721 cagcaccgcc gtggatatcc tgccctggca gccagtcgtg gccgatctgg ccgacggcgc
     3781 gggaaccaag accgtggtcc cgacgtcgat tcagctcaag gtggacggcg tggtggccgc
     3841 tatcattaag accaatccca ccccgggaat cactcacatt atcgagcagg tggggatggc
     3901 ccggagtgcg accgcgcaca caaatgtcct gacctatacg gacaactcgg gcgcttctta
     3961 caccaacacc tgggccttca ccgtgttgaa caacttgccg ttcggcatca cgacgttgcc
     4021 gctcgccagc atgcgcccca ccaaccaggt ggatcacagt cagcccggct tccgcgtgaa
     4081 ggcttatcag accaccgcgg cttccccgcc caatgtgaca tttgccgagg aagccttcgc
     4141 cggcttgcat ggggccaata tcgccactgc ggtggccacg cccccggagg gatacttcgt
     4201 ctggaataac gaaatggatt ttgcggacga catcgccgcc aacgccggca acggcgaata
     4261 tcgctacaac tacttcctca gccagtattt tggatacgtg ctccagggcg gcggcctcgc
     4321 cgtcaactat aacgaagcca tcttcgccgg ctggatggaa ttccccaccg caggcctcta
     4381 tgccatgacc gtcaaccgcg atgacggttt ccggctgact tgcccctatg gcagcaatcc
     4441 cttcgaggag gcgggccagg ttgtcggagt cttcgatggc tcgggcggca acggcggcgc
     4501 ggcctatccg ccgaccgcgg gcctccggtt tgtcttcaac gtccctgccg cgggcgcgta
     4561 tccgttgcgc ctgatctggt ataacggcac gggtggtgcc agcctcgagt ggtcagtctg
     4621 gcagtatctg cccgatggca gcatggcccg cctgttggtc aacgacccca acacccttgg
     4681 cgccctcaag gtttaccaga ccctcaccaa cggctccgcc ccgtatgtgg cctttatcaa
     4741 cccatacccc gggaccgctt taggccagca ggccaatgtc ttctggcaat caactcccat
     4801 ccccggctcg acctttgttc cagccgtcag cgaggctgac ctcaccgtcg ccctgcagga
     4861 tggggccacc acggtcctta ccaatacgat taccttgacc tttaacggcg tggtccagcc
     4921 cattaccatc ggtacaagca acggcctcac caccatcacc cgggcggcga ccaacggctt
     4981 ctggccgtcc ggtggcctcg ggccgctggt gctgagcttc accgatagcg cgagtaacac
     5041 ctactcctac cccgtcgaaa tcctggccac gccgttctgg ggcacgctga ccaatgcggt
     5101 taccgcccag gtggatacca acaagccggg cttcaagatg cggatctatc aactggatcc
     5161 taaggcgacg gcgggcacca ccgttatccc cacccgggtg catgtcgccg agcaggtgct
     5221 cagcggggtc tggggcacca acgccgtcaa gcccaccgcc tacaccaaca atggttatgt
     5281 caatctccag ggcaccggcc ccactgccgg caccatcaac ttcaatcttc tcggcgccgc
     5341 ccagatcggt gacttccagt ccggtggcgg ctttgccgac cagatctggc ccggcgttcc
     5401 cggcttcatc accgccacgg tgaactcgga taccaacaac gacttcgccg ccgagattct
     5461 ggcgtacgtt gaattcccaa ccaatgggac ctacttcctg gccgtcagca gcgacgatgg
     5521 cttccggctc tggaacggct ggacaccgcc ggtcaacaaa ggcgctctgg ttgtgaacag
     5581 cccggtcgct ttagccggaa tcaaagccac cgtcatcaac ggcagcacgg ctaatactcc
     5641 gttcatctcg ctgccgctga ccaacgccat caatggcgac ctggtgctgg cccaaggcat
     5701 cggcttcggc agcaccacca acggcgaggg ttgtgtgatt actaacccgg gccagttggc
     5761 gggcaagatc gcagtgatgt accggagcgc cttctgcggc tatgcccagc aagtcgccaa
     5821 cgcgcaagcc gccggcgccc tcgccgtcgt cctcatccaa aaccgcccgg ccacggaagg
     5881 gccgttcccg caagaaccgg ccatctctcc ctgggcagcc atcccggcca tccagatcga
     5941 gcaggctgat ggcaacgctc tggtcgccgc cctcaatggc ggcaccaacg tgaatgtgac
     6001 cctcacgccg ctg
//
LOCUS       DATCGS010000216         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_514850, whole genome shotgun sequence.
ACCESSION   DATCGS010000216 DATCGS010000000
VERSION     DATCGS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514850"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3304)
                     /locus_tag="VIL39_04345"
     CDS             complement(<1..>3304)
                     /locus_tag="VIL39_04345"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY5480686.1"
                     /translation="WVYWFWLDGNITREGITADLEAMRRVGIGGVLIMEVDQGAPKGP
                     ARFASPQWRELFKFMLAEANRLGLEVNMNNDAGWCGSGGPWITPALAMQKVVWTNVTV
                     EGPRRLEQVLPEPQKVANYYEDIAVLAFPTPADYRIKDILAKAAFIRQEVPPQKLPEP
                     LPAEKTIPRGSIRELTAQMSKDGRLVWDVPPGKWTVVRFGHTPTGKDNHPAPLDGRGL
                     ECDKLNRKAADAMFEGLMGKLIADSKPLAGKTLVATHIDSWEVGSQNWTPAFREEFGR
                     LRGYDPLPFLPVMTGRVVDSLDISERFLWDLRQTISELVVKNYAGRFRELAHQHGLRL
                     SIEAYDGCCDDMTYAGQADEPMGEFWSWTFGSGADWCTEMASAAHIYGKPILGAEAFT
                     ATDKEKWLGHPGGIKALGDWAFCEGINRFVFHRYALQPWRDVKPGMSMGPWGLHYERT
                     ETWWDQSTAWHEYLSRCQFLLQQGQFVADICYLEPEASPYRFRAPPTPGSPPHRPAYN
                     FDGCTPEVVLTRMKVKDGRLVLPSGMSYRLLVLPEVETMTPKLLRKVSELVKAGATVV
                     GPAPLRSPGLSNYPQCDDEIQKLTGKLWSGGAGEGTAAFGRGQVIWPIERRRDVVAQE
                     TPARLGSAQWIWFKEGRPAVSAPVGTRYFRRIVNVENGAGIESARLVMTADNEFEAWV
                     NGRRAGGGDDFSHTYVMDCTRLLQPDTNLLTVAAVNGSTSPNPAGMVGNLIIKFQDGR
                     TVELHTDGTWEAAKTAEGDWRKATAAPGAWKPAMELGAVGMAPWGEIDHPTGGAKTLY
                     PNAEVIAGLMKRMGVPPDFDYVTHSSERDLRYIHKRIGKVDLFFVANEKPHSEQAVCS
                     FRVEGKSPELWWPDTGRIERAAVYDEAGGCVRLPLRFDASGSVFIIFRPGETIEPDRV
                     TSMKRNGELLADLATKELAAATVAEPGIEIVRGTGGTVEAQVWQAGTYTLTSANGKSR
                     QLAAASIPEPVEITGSWELRFPAKAGAPERVTLDRLISWSEHTDPGVRHFSGRATYLK
                     TLNVPTGLIAPGRRLYLDLGKVEVMASVRVNGKDLGILWKQPYCVEVTDALKPGANTL
                     EVQVANLWINRQ"
BASE COUNT          568 a         1177 c          940 g          619 t
ORIGIN      
        1 atctgccggt tgatccaaag gttggccact tgcacttcga gcgtgtttgc tcccggcttc
       61 aaggcgtcgg tcacctccac gcaatacggc tgcttccaca ggatgccaag gtctttgccg
      121 ttgaccctga cgctggccat gacctccacc ttgcccagat cgagatagag ccgccgccct
      181 ggtgcgatta gcccggtcgg cacgttcaac gtcttcaaat aagtcgccct cccggagaag
      241 tggcgcacgc cgggatcggt atgctcactc caggaaatca accggtcgag cgtcacccgc
      301 tcgggcgcgc cggccttggc cgggaaccgc agctcccacg agccggtgat ctcgaccggc
      361 tcgggaatcg aggccgccgc caactggcgg ctcttgccgt tggcactcgt caacgtatat
      421 gtccctgcct gccagacctg ggcttcgacc gtgcctcccg tgcctcggac gatctcaatc
      481 ccaggctccg cgaccgtcgc cgcggcaagc tccttcgtgg cgaggtcagc cagcagctcc
      541 ccgtttcgct tcatagatgt gacccggtcc ggctcgatag tttctccagg acggaagata
      601 atgaacactg aaccgctggc gtcgaagcgg agcggcaacc tcacgcaacc gcctgcctcg
      661 tcataaaccg ccgcccgttc aatccggcca gtgtccggcc accacagttc ggggctcttg
      721 ccttcgacgc ggaagctgca tacggcttgc tcggaatgcg gcttctcgtt ggccacaaag
      781 aacaggtcca ccttgccaat ccgtttgtgg atgtaacgca agtcccgctc cgagctgtgc
      841 gtcacgtaat caaaatccgg cggcacgccc atcctcttca tcaagccggc aatcacctcg
      901 gcattcggat agagagtctt cgccccgccg gtaggatgat caatctctcc ccaaggcgcc
      961 atgcccactg cgcccagttc catcgccggc ttccaggcgc cgggcgcagc cgtcgctttg
     1021 cgccagtcgc cctcggctgt tttggcggcc tcccaggtgc cgtccgtgtg cagctctaca
     1081 gtgcgaccgt cctggaattt gataataagg ttgccgacca tgcctgccgg gttgggggaa
     1141 gtggaaccgt tcacagcggc caccgtcagg agatttgtgt ccggctggag cagccgcgtg
     1201 cagtccatca cgtaggtgtg tgagaagtcg tctccaccgc cagcgcggcg gccgttaacc
     1261 cacgcctcga actcgttgtc ggcggtcatc accaggcggg ccgattcaat cccggccccg
     1321 ttctccacat tcacgattcg gcggaagtag cgcgtgccca ccggcgcgga cactgcaggt
     1381 ctgccttcct tgaaccaaat ccactgcgcc gaaccgagcc gcgctggcgt ttcctgagcg
     1441 accacgtcgc gccttctttc gatcggccag atcacttggc cccgcccgaa cgcagcggtg
     1501 ccctcccctg ccccgccgct ccatagcttc cccgtgagct tctgaatctc gtcgtcgcac
     1561 tgcgggtaat tcgagaggcc cggcgatctc agcggcgcgg gtcccaccac cgtcgcgccg
     1621 gctttgacca actcgctgac cttgcgcagc agcttcggcg tcatcgtctc cacctcgggc
     1681 agcacgagca ggcggtaact catgccgctg ggcaacacga gccggccatc cttcactttc
     1741 atgcgcgtga gcaccacttc cggcgtgcag ccgtcgaagt tataggcggg ccgatgaggc
     1801 ggactcccag gggtgggcgg ggcgcgaaaa cgatagggcg aggcttccgg ctccaagtag
     1861 caaatgtcgg cgacaaactg cccttgctgg agcaggaact ggcagcggga caggtactcg
     1921 tgccaggctg tggactggtc ccaccacgtc tcagtccgct cgtaatgcag cccccatggg
     1981 cccatggaca tccccggctt cacatcccgc cacggctgca gcgcgtagcg gtgaaacacg
     2041 aatcggttga tcccctcgca gaacgcccaa tcgcccagcg ccttgatgcc gcccggatga
     2101 cccagccact tctccttgtc ggtcgccgtg aacgcctcgg cccccaggat gggtttgcca
     2161 taaatgtggg ccgccgatgc catctccgtg caccaatcgg cgccgctgcc aaaggtccat
     2221 gaccagaact cacccatagg ctcatcggcc tggccggcgt aagtcatatc gtcgcagcag
     2281 ccatcatacg cctcgattga aagccgcagc ccgtgctggt gtgccagctc gcggaaccgg
     2341 cccgcgtagt ttttcaccac cagctctgaa atcgtctgcc gcaggtccca gaggaaccgc
     2401 tcggagatgt ccaggctgtc caccacccgc cccgtcatca ccggcaggaa gggcaaagga
     2461 tcgtagccgc gcagccgtcc gaattcctcc cgaaaggccg gggtccagtt ctgcgacccc
     2521 acttcccagc tatcaatgtg cgtcgccacc agcgtcttcc cggccagcgg cttggagtcc
     2581 gcgataagct tgcccatcag cccttcgaac attgcgtccg ccgctttgcg gttcaacttg
     2641 tcgcactcca gcccacggcc atcgagcggc gcgggatggt tgtccttgcc ggtcggcgta
     2701 tgcccaaaac gcactaccgt ccatttgccc ggtggcacgt cccacaccag ccggccatcc
     2761 ttggacatct gcgcggtaag ctcccggatg ctgccgcgcg ggatggtctt ctccgccggg
     2821 agcggctccg gcagcttctg cggcggcacc tcttgccgaa taaatgccgc cttggccagg
     2881 atgtccttga tgcggtaatc cgccggagtc gggaaagcca gcacggcaat gtcctcgtaa
     2941 tagttcgcca ccttttgcgg ctcaggcagc acctgctcga gacggcgcgg cccttcaacg
     3001 gtcacgttcg tccacaccac tttctgcatg gccagcgcgg gggtaatcca cggcccgccg
     3061 ctgccgcacc agccggcgtc gttgttcatg ttcacctcca ggccgaggcg gttggcctca
     3121 gccagcatga acttgaacag ctcgcgccat tgcgggctcg caaaccgcgc gggacctttg
     3181 ggcgcgccct ggtccacttc catgatcagc accccgccga tccccacccg ccgcatcgct
     3241 tccaggtcgg ccgtgatgcc ctcgcgcgtg atattcccat ccagccagaa ccaatagacc
     3301 cacg
//
LOCUS       DATCGS010000217        10268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_712614, whole genome shotgun sequence.
ACCESSION   DATCGS010000217 DATCGS010000000
VERSION     DATCGS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712614"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="hydG"
                     /locus_tag="VIL39_04350"
     CDS             complement(<1..1348)
                     /gene="hydG"
                     /locus_tag="VIL39_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HEY5480687.1"
                     /translation="MTTTRSHQLATYIDEAKISATLAAARTCDAKQVREILAKAREMK
                     GLDASDMATLMENNDPELLEEMYAAARHVKNEIYGNRLVLFAPLYISNLCHNECLYCA
                     FRARNKELVRRTLNPEEIANETRILIEQGHKRVLLVSGESYPKEGFDYVLNAIATVYS
                     TRSGKGEIRRVNANVAPLSVEGYKRLKAAKIGTYQCFQETYHRATYAAVHVSGIKRDF
                     DWRVTCFDRAMQAGIDDVGIGVLFGLYDWKYEVLAMKQEIEHLEDAFGVGPHTISVPR
                     MEPAVGSTLAYAPTHPVSDSDFKKIVALLRLAVPYTGIIMSTRENAETRRGTFELGVS
                     QISAGSRCDPGGYAASANGHKLEQFQLGDHRSLDEVVRDVAALGYTPSFCTACYRLGR
                     TGADFMDMAKPGLIKHHCAPNALSSCMEYLLDYAKPATRKVGESLIAHELETMEPSV"
     gene            complement(1373..3400)
                     /locus_tag="VIL39_04355"
     CDS             complement(1373..3400)
                     /locus_tag="VIL39_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEY5480688.1"
                     /translation="MTTTVATKTLTIDGREVAFNGERNLLELIRKAGIDLPTFCYHSD
                     LSVYGACRLCLVDIQGRGVQGACSTPPEAGLKVRTQTEEIREIRKITIELLLANHDQQ
                     CPTCPKSSACQLQDIARKLGIRKVRFKPVHQPAELDLSSVSLVRDPNKCVLCGDCVRF
                     CSEIQGVGAIDFAHRGHDAAVLPAFGKGLGEGECINCGQCAAVCPTGALTPRPEVDEV
                     WRALNDPVKTVVVQVAPAVRVAIGEAFGLAPGLSSSGQIVAALKRLGFDKVYDTAFSA
                     DLTVIEEANEFLKRKAKGEKLPQFTSCCPGWVKFAEQYFPDLLPNLSTCKSPQQMLGA
                     VCKATLPGEMGIPKENLVMVSIMPCTAKKFEAKRPEFAEGGVPEVDHVLTTQELARMI
                     EEAGFRFKELEPESFDMPLGFKTGAGVIFGNSGGVSEAVLRYAAETVTGRKLDNPDFL
                     AVRGEDGVRFATVALDGVQLKLAVVHGLKNARTLAEKVRRGECELDLIEVMACPGGCI
                     GGAGQPVSRERDVKRLRTRGLYDADKHLDLHKSQENHHVTEMYQKYLGEIGGRKAHHL
                     LHTHYQNRRRIADERMVFGDGPGAEKVKVSVCAGTNCFLKGSQEVIRDVLQCVDAQEL
                     QDQVEVSASFCFEKCDQGPTVSVDGQQIQHCNGQKACAAMRERLKNKVAPE"
     gene            complement(3454..5337)
                     /locus_tag="VIL39_04360"
     CDS             complement(3454..5337)
                     /locus_tag="VIL39_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5480689.1"
                     /translation="MSAQSSTNPNGNNGHSAQRRVIVCAGTGCVASGSYKVFQALVEQ
                     VRAAGLPVVTEFRPEPKAGAVRLNKSGCQGFCQMGPLVTVLPENLLYNKVRLEDVPEI
                     VTETLVAGRVVDRLLYVDSTTKKHCRGVEEIPFYQRQQRFVLHSCGTIDPESIEEYEH
                     QGGYAAAKKAYLQMTPEQICQEISRSGLRGRGGGGFPTGRKWEAARSQPEGKKYVICN
                     ADEGDPGAFMNRSVMEGNPHSVLEGLMIAARAIGADETLIYVRTEYPLAVQRMRTAVK
                     DAEARGFLGDNAFGSGRPLRCEVMEGAGAFVCGEETAMIASIEGQRGMPRPKPPFPAQ
                     SGLWGKPTIINNVETLAHAVRIINEGAEQFRKLGTAASPGTKTFALTGHVANTGLVEV
                     PFGTTLREVVFDIGGGITDRRGKLTRDGFKAVQIGGPSGGCLTRAHFDLPLDYDSLRG
                     AGAMVGSGGLVAMNNKTCMVAIARFFMDFTQRESCGKCVLCREGTRQMLALLDDIIEG
                     RATAETLDLLQKLASAVQKGSLCGLGKTAPNPVLSTLRHFREEYDAHVFHKHCPSGQC
                     KALAKPSIDPAKCKGCTACAKKCPVHAISGERKQAHTIDQALCIKCGVCADTCKFEAV
                     LGV"
     gene            complement(5334..5837)
                     /locus_tag="VIL39_04365"
     CDS             complement(5334..5837)
                     /locus_tag="VIL39_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5480690.1"
                     /translation="MTAKRKPESTGRRFVKVCHILDQHGHNPSRLVPILQAVQEEYRY
                     LPQEVLTYVATALGVSPARVYGVATFYAHFTILPKGKHLVRLCDGTACHVKDSVPILR
                     ALQKRLGVDDRRKTTEDMLFTVETVACLGACGLAPVVVIDDQVHGQQTPESAVKLVEA
                     LITQEAQ"
     gene            complement(5865..6560)
                     /locus_tag="VIL39_04370"
     CDS             complement(5865..6560)
                     /locus_tag="VIL39_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY5480691.1"
                     /translation="MNTEMPRTVSEATLKRLPLYHRFLKELLQREQEAVSCTDIGEEL
                     KLDPTQVRKDLEMAGISGRPRIGYATAAVVDGIERFLGWKKASEAFLVGAGSMGSALL
                     GYRKFEECGLKIVAAFDSDPAKIGRKIHGKHVLALGKLADLTQRMHVLIGIITVPAEQ
                     AQAVADVLVEGGVRAIWNFAPLRLRVPEHIIVHNEDLYCSLASLSQKLGQALETNPAE
                     PVTARRQRTAILD"
     gene            6899..7654
                     /locus_tag="VIL39_04375"
     CDS             6899..7654
                     /locus_tag="VIL39_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY5480692.1"
                     /translation="MKAQAGTSKRARQARTSPSPAVESAVTPAGTPSAEAVQVAGVAC
                     VKRLPAYLQMLRVLQGEGQEYVSGTLLATTHSLEPVIVRKDLAVTGAAGIPRRGFRIA
                     ELIAAIERFVGWDNQTKAVLVGVGNLGTALLGYQGFENLGLRIAGAFDRDPAKVGKWI
                     QGRKVQPISQLAAFVRRGGIRLGVLTVPAAVAQETADLMIRAGIRGIWNFSPARLEVP
                     AGVVTQKEDLAEGLAVLSHKLQHLPPADPAKLG"
     gene            8030..10105
                     /gene="ligA"
                     /locus_tag="VIL39_04380"
     CDS             8030..10105
                     /gene="ligA"
                     /locus_tag="VIL39_04380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY5480693.1"
                     /translation="MNLNEARARHAQLVEEIRRHDYAYYVLAQPTIGDRDYDRLYHEV
                     LDLEQQFPQLVTPESPTQRVSGQPLKEFKQVQHLLPMLSLDNTYSQADVRDFVNRVQK
                     LLPEEKLEWIVEPKIDGVAINLRYEQGVFACGSTRGDGTTGDDITPNLKTIRSIPARL
                     HSGAGVSPVSGAGDRRDACPTMLEVRGEVYLTKTGFEKLNAERKAAGEEVFANPRNAA
                     AGSLKQLDPRIAAKRPLDIVVYGLGQLEGAPQPPQKHDQMLDWLKSLGFKTPDRTWHC
                     RSADELVAAIEALDQVRREFAYETDGAVIKLNSFAQREIAGFTSKAPRWSIAYKYAAE
                     QAETRLNGITVQVGRTGALTPVAELEPVFLAGSTISRATLHNEDYVRQKDIRIGDTVK
                     IEKAGEVIPAVVDVVLARRTGKEVSFRFPRQCPECGSKVSRAPGIVEGDEGVVWRCLN
                     PDCPAQVRGRLEHWCARGAMDIEGGGEVLARQLVQNGLVRDVAELYSLKLSDVAGLER
                     MGEKSARNFLDGVEASKQRDLWRVLYGLGILHVGAGVAKALGRGFATLDDVFAASVDQ
                     LTDCEDIGDVIAQSLVQWQGDSRNQKLIERLRKAGLNSKSALYQPKAKAGPLTGKTFV
                     LTGTLPTLKREEAAAKIEAAGGKVSGSVSKKTDYVVAGAEAGSKLDKAQALGVKIIDE
                     AELLRLCGA"
BASE COUNT         1844 a         3301 c         3134 g         1989 t
ORIGIN      
        1 gcactgacgg ctccatcgtt tccaactcat gggcgatcag gctctcgccc accttgcgag
       61 tcgccggctt cgcatagtcc agcagatact ccatgcagct cgacagcgcg ttgggggcgc
      121 agtggtgttt gatcaggccg ggtttcgcca tgtccatgaa atcggccccg gtgcggccca
      181 gccgataaca ggccgtgcag aaggaaggcg tatagccgag cgccgccacg tcgcgcacta
      241 cttcatcgag cgagcggtga tcgcccaact ggaactgctc caatttgtgg ccgttagccg
      301 aggcggcata gccgcccgga tcgcaacggc tgcccgccga gatttgggaa acgcccagct
      361 cgaaggttcc ccggcgcgtc tcagcgtttt cgcgcgtgga catgataatg ccggtgtaag
      421 gcacggccag acgcaggaga gcgacgatct tcttgaagtc gctgtccgag accgggtgtg
      481 tcggcgcgta ggccagagtc gaaccgaccg ccggctccat tctcggcacg ctgattgtgt
      541 gcgggccgac gccgaaagcg tcttcgaggt gctcgatctc ctgtttcatc gcgagcactt
      601 catacttcca atcgtagagg ccaaacagga ccccgatccc gacgtcatcg atgccggcct
      661 gcatcgcgcg gtcaaagcag gtcacccgcc agtcgaaatc ccgcttgata ccgctgacgt
      721 gcacggcggc gtaggtcgcg cgatgatagg tctcctggaa gcattggtat gtgccgatct
      781 tcgccgcctt gagccgcttg tagccctcga cactgagcgg cgccacgttg gcattgacgc
      841 ggcggatttc tcctttgccg ctgcgggtgc tatagaccgt ggcaatcgcg ttcaagacat
      901 aatcgaagcc ctccttcgga tagctttccc cggagaccag caacacccgc ttatgcccct
      961 gctcaatcag gatgcgcgtc tcattcgcga tctcctccgg gttcaacgtc cgccggacca
     1021 gttccttatt gcgcgcgcga aacgcgcaat agaggcactc attgtggcac aggttcgaga
     1081 tatacagcgg ggcgaatagc accaggcggt tgccgtagat ctcgttcttc acgtgccggg
     1141 ccgcggcata catctcctcc aacaactccg ggtcgttgtt ctccatcaag gtcgccatgt
     1201 cggaggcgtc caggcccttc atttcgcgcg ccttggccag aatctcgcgg acctgcttgg
     1261 catcgcaggt tcgggcggcg gccagcgtcg ctgagatttt ggcttcgtcg atgtaggtgg
     1321 ccagttgatg ggaacgtgtt gttgtcataa aagatgtatc gccgtggcgc gctcactcgg
     1381 gcgcgacttt gtttttgagg cgttcgcgca tggcggcgca cgccttctgt ccgttacagt
     1441 gctggatttg ctggccgtcc acgctgaccg tcgggccctg gtcgcatttc tcgaagcaga
     1501 acgaagcgct cacttcgacc tggtcttgca gttcctgggc atcgacgcat tggagcacgt
     1561 cgcggatgac ctcctgcgac cctttcagga agcagttggt gcccgcgcac acgctgacct
     1621 tgaccttttc cgctcccggt ccgtccccga ataccatccg ctcatccgca atacgccgcc
     1681 ggttctgata atgcgtgtgc aagaggtgat gggccttgcg cccgccgatc tcgcccagat
     1741 atttctggta catctcggtc acgtgatggt tctcctgcga cttatgcaga tccagatgct
     1801 tgtccgcatc atacaggccc cgggtgcgca agcgcttgac gtcgcgctcg cgtgacaccg
     1861 gctggccggc gccgccgatg cagccgcccg ggcaagccat cacctcgatg aggtccagct
     1921 cgcattcacc ccggcgcact ttctccgcca gcgtgcgggc attcttcaat ccgtgaacca
     1981 cggccagctt gagctgaact ccgtccaaag cgacggtggc gaagcgcact ccgtcttcgc
     2041 cgcgcactgc gaggaagtcc ggattgtcca gcttccgccc cgtgactgtt tcggcggcat
     2101 accgcaggac ggcttcgctc accccgcccg agttgccgaa gatgacgccg gcgccggtct
     2161 tgaagccaag cggcatatcg aaagattccg gctccagctc tttgaagcgg aacccggcct
     2221 cttcgatcat ccgcgccagc tcctgcgtcg tcaggacatg atccacttcc ggcacgccgc
     2281 cttcggcgaa ttcgggccgc ttggcctcaa acttcttggc ggtgcagggc atgatggaaa
     2341 ccatgacgag gttctccttc ggaatgccca tctcgccggg cagcgtggcc ttgcacacgg
     2401 cgccgagcat ctgctgcggc gatttgcacg tggacaaatt cggcagcagg tccgggaaat
     2461 actgttcggc gaatttgacc cagcccgggc agcaactggt gaactgcggc aacttctcgc
     2521 ctttggcctt gcgcttcagg aattcattgg cttcttcaat caccgtcagg tcggcgctga
     2581 aggcggtgtc gtaaaccttg tcgaagccga ggcgtttaag cgccgcgacg atttggccgc
     2641 tggacgacag gccgggagcc agcccgaaag cctccccgat cgccacccgc actgccgggg
     2701 caacttgcac gaccaccgtc ttgactggat cgttgagcgc acgccacacc tcgtccacct
     2761 cgggccgggg cgtcagcgcg ccggtggggc agaccgcggc gcattggccg cagttgatgc
     2821 actcgccctc gcccaatcct tttccaaagg ccggcagcac ggcggcgtcg tgtccgcgat
     2881 gggcgaagtc gatggcgccc acgccctgga tctccgagca gaaacgcacg caatccccgc
     2941 agagcacgca cttgttcgga tcgcgcacca gcgagaccga ggagaggtcc agctcggcgg
     3001 gctgatgcac aggtttgaaa cgcactttgc ggatgcccag cttccgggcg atgtcctgga
     3061 gctggcaggc cgagctctta ggacaggtcg ggcattgctg gtcatggttg gccagcagca
     3121 gctcgatcgt tatcttacga atctcgcgaa tctcttccgt ctgcgtgcgc accttcaggc
     3181 ccgcttcggg aggggtcgag caggcgccct ggacgccgcg gccctggatg tccaccaggc
     3241 agaggcggca agccccatac acgctcaggt ccgagtgata gcagaacgtc ggcaggtcga
     3301 tgcccgcttt tcgaatcagc tccagcaaat tgcgctcgcc gttgaacgcc acttcgcgcc
     3361 cgtcaatggt caatgtcttt gtagctacgg tggtagtcat gttgccttca aatggtttcg
     3421 tttcagtcga tttcatgctt tggtcgcggc gggctatacg ccgaggacgg cttcgaactt
     3481 gcaggtgtcg gcgcagacgc cacatttgat gcacagcgcc tggtcaatcg tgtgggcctg
     3541 tttccgttcg ccgctgatgg catgcacggg acacttcttg gcacacgcgg tgcagccctt
     3601 gcatttggcc gggtcgatgc tgggcttggc cagcgccttg cattggccgc tgggacaatg
     3661 cttgtgaaac acgtgcgcgt cgtattcctc gcggaagtga cgcagcgtgg agaggaccgg
     3721 attgggagcc gtcttgccca gcccgcagag tgagcctttc tgcacggccg aggccagctt
     3781 ctgcagcagg tccaaggtct ccgcggtggc gcggccctcg atgatgtcat cgagcaacgc
     3841 cagcatctgc cgcgtccctt cccggcagag cacacatttg ccgcagctct cccgctgggt
     3901 gaagtccatg aagaaccggg cgatggccac catgcaggtc ttgttgttca tcgccaccag
     3961 tccgccggag ccaaccatcg ccccggcgcc gcgcaatgaa tcgtaatcga gcggcaggtc
     4021 aaagtgcgcg cgcgtgaggc atccgccgga gggcccgccg atttgcaccg ctttgaaacc
     4081 gtcgcgggtc aatttgccgc gccggtcggt aatgccgccg ccaatgtcga acactacttc
     4141 ccgcagcgtg gtcccgaaag gcacttccac cagccccgtg ttggccacat ggccggtcag
     4201 cgcaaaggtc ttggtgcccg gcgatgccgc cgtgcccagc tttcggaatt gctcggcgcc
     4261 ttcattgatg atgcggaccg cgtgggcgag cgtttccacg ttgttaatga tagtcggctt
     4321 gccccaaagc ccgctctggg ccgggaatgg cggcttgggc cgcggcatgc cgcgctgccc
     4381 ctcgatcgag gcaatcatcg ccgtctcttc gccgcacaca aaagcccccg cgccttccat
     4441 cacctcgcag cgcagcggac ggcccgagcc gaaggcgttg tcacccagga aaccgcgtgc
     4501 ttccgcgtcc ttgacggcgg tgcgcatgcg ctgcacggcc agcggatact cggtccggac
     4561 gtagatcagg gtttcgtccg cgccaatcgc gcgcgcggcg atcattagcc cctccagcac
     4621 gctgtggggg ttgccttcca tcacgctgcg gttcatgaac gcgcccgggt ctccctcgtc
     4681 cgcgttgcag atcacatatt tctttccctc aggctggctc cgtgcggctt cccacttgcg
     4741 cccggtgggg aaaccaccac cgccgcggcc gcgcaggccc gagcggctga tctcctggca
     4801 aatctgttcc ggcgtcatct gcaagtaggc cttcttcgcc gccgcatagc cgccctggtg
     4861 ctcgtactcc tcgatgctct ccggatcaat cgtgccacag gaatgcagca caaaccgttg
     4921 ttgccgttgg tagaacggaa tctcttccac gccccggcaa tgcttcttgg tcgtcgagtc
     4981 cacgtaaagc agccggtcaa ccactcgccc ggcaaccagc gtctcggtga caatctcagg
     5041 cacgtcttcc aaccgcactt tgttatagag caggttctcg ggcagcacgg tcacgagcgg
     5101 ccccatctgg cagaagccct ggcagccgct cttgttcaga cgcaccgcgc cggctttggg
     5161 ctccggccga aactcggtga cgaccgggag gccggcggcc cgcacctgtt ccaccagggc
     5221 ctggaagacc ttatatgacc cgctggcgac gcagccggtt cccgcgcaca caatgacgcg
     5281 tcgttgggcc gagtggccgt tgtttccgtt tgggttggtt gaactttgcg cgctcattgg
     5341 gcctcctgtg tgataagggc ttcgaccagc ttgaccgcac tctcgggcgt ttgttgcccg
     5401 tgaacctggt cgtcgatcac gacgaccggt gccaggccgc aggcgcccag gcacgccacg
     5461 gtctccaccg tgaacagcat gtcctcggtg gtcttccgcc gatcatctac tccgagccgc
     5521 ttttgcagcg cgcgcaggat cggcaccgag tccttgacgt ggcaggccgt gccgtcgcag
     5581 agccgcacca ggtgtttgcc cttgggtaaa atagtgaagt gcgcgtagaa ggtcgccacg
     5641 ccataaacgc gcgcgggcga tacgcccagc gccgtcgcaa cgtaggtcag cacctcctgc
     5701 ggcaaatagc ggtattcctc ctgcactgct tgcaggatgg gcaccagccg cgacgggtta
     5761 tggccgtgct ggtccaggat gtggcacact ttgacgaatc tgcgcccggt gctttcaggc
     5821 ttacgtttag cggtcatagg catttggatt tttctagtaa gtgtttaatc taagattgca
     5881 gtccgctgtc tccgggcggt caccggctcg gcggggttgg tctccagcgc ctggcccagc
     5941 ttctgggaca gcgacgccag cgaacaatag aggtcttcgt tatggacgat gatatgctcc
     6001 ggcactcgca gccggagcgg ggcaaaattc cagatggcgc gcacgccgcc ttccacgagc
     6061 acgtccgcca ccgcctgcgc ctgctcggcc gggacggtga tgatgccgat gaggacgtgc
     6121 atccgttggg tcaggtcggc cagtttgccc agcgccagca cgtgcttgcc gtggatcttc
     6181 cggccgatct tggccgggtc actgtcaaag gccgccacga tcttcaggcc gcattcctcg
     6241 aatttccggt agcccagcag ggccgagccc atgctgcccg cccccacgag aaaagcctcg
     6301 ctggccttct tccaccccag gaatcgctcg atcccgtcca cgaccgccgc ggtggcatag
     6361 ccgatgcgcg gccggccgct gatcccagcc atctccaggt ccttgcgcac ctgcgtcgga
     6421 tccagcttca gctcttcgcc aatgtcggtg cacgatacag cttcctgctc gcgttggagc
     6481 agttccttta gaaaacgatg atacagcggc aggcgcttga gcgtcgcctc ggacaccgtc
     6541 ctgggcattt cagtattcat gactcgatgc gagaatcttt aatcgatacc aatgtatcga
     6601 gaattatgta ttgatatata aatcacgaat agccaggtgc gtcaaccgat gagtccaatt
     6661 aggggttggc cccgcactcg gcctttcttc tccgtggtgg ttcgcaaacc cctgtccctg
     6721 ctgcatgttt ctgcgttggc taacgccgtc gtcccggcca gccgcgccaa gaaatgtaag
     6781 aagaatggca atccaggttg acgtcttctg gcgaacactt ctggcgaaca cccttgcccc
     6841 tggttgcgag tgtcggctaa tctcaagcgg cagccggcat ggccgccata tttatgcgat
     6901 gaaggcacaa gcgggcacta gcaagcgcgc gcggcaggcg cgaacaagtc catctccggc
     6961 agtcgaatcg gcggtgacac ccgccggtac gccctcggcg gaagcggtgc aggtggcggg
     7021 cgttgcatgc gtgaagcgcc tgccggctta cctgcagatg ctgcgcgtgc tccagggcga
     7081 agggcaggag tacgtctctg gcaccctctt ggcgaccacg cattccctgg aacccgttat
     7141 tgtccgcaaa gacctggctg tgacgggcgc ggctggaata cccaggcgcg ggtttcggat
     7201 cgcggagttg atcgccgcca tcgaacgctt cgtgggctgg gataaccaga ccaaggcggt
     7261 cctggtgggc gtgggcaacc tgggcacagc actcctgggc taccagggat tcgagaatct
     7321 cggcttgcgg atcgccggcg cctttgatcg ggaccctgcg aaagtcggca agtggattca
     7381 gggacggaaa gtgcagccca ttagccaact ggcggctttc gtcagacgtg gcggtattcg
     7441 gctgggtgtc ctcaccgtgc ccgccgcggt agcgcaggag acggccgatc tcatgatccg
     7501 cgcgggcatc cgcggcattt ggaatttcag ccccgcccgg ttggaagttc cggcaggcgt
     7561 cgttactcag aaagaagatt tggcggaggg cctggccgtc ctttcacata agttgcagca
     7621 cttgccccca gccgaccccg ccaagctggg ttaggcggag ccgcgcacga gcccccattc
     7681 acctggtaac ccagccgcgc tggcccggga gcccgcggat tcttcatggt ctaccccgac
     7741 tttgctgttt gagattgtgt agctgactgc gcgagccagg atgaggtgcg tgcagattcc
     7801 cgcatggaac gccaactgcg acggaacctg acgggcgctt cctgacacca ccaccggaac
     7861 gccggattca tccggcagac cggtgagccg cactggggct gccggatgaa tccggcgttc
     7921 cgattggcat agccgcccgc cttggtggtg gtgtcaggat gcgcacgaac ctggcgacat
     7981 ctcccttttc tgcgtgcatt tttagccggt cggactacta atgctgccga tgaacttgaa
     8041 cgaagccaga gctcgtcacg cccaacttgt tgaggaaatc cgccggcacg actatgccta
     8101 ttacgtgctg gcgcagccca ccatcggcga ccgcgactac gaccgactct accatgaagt
     8161 gctcgacctg gagcagcagt tcccccagct cgtcacgcca gagtccccca cgcagcgcgt
     8221 cagcggacag ccgctgaaag agttcaagca ggtgcagcat ctgctgccca tgctcagcct
     8281 ggacaacacc tattcgcaag cggacgtgcg cgatttcgtc aaccgcgtcc agaagcttct
     8341 gccggaggaa aagctggagt ggatcgttga gccgaagatt gatggcgtcg ccatcaacct
     8401 gcgctacgag caaggcgtgt ttgcctgcgg ttcgacccgc ggcgacggca cgaccggcga
     8461 tgacatcacc cccaatctta agaccatccg gagcatccca gcccggcttc atagtggggc
     8521 aggcgtctcg cctgtctctg gggccggaga caggcgagac gcctgcccta ctatgctgga
     8581 agtgcgcggc gaggtttacc tgacgaagac gggcttcgaa aagctgaacg cggaacgcaa
     8641 agccgccggc gaggaggtat ttgccaatcc gcgcaatgcg gccgccggct cgctcaagca
     8701 gctcgacccg cgcatcgccg ccaagcgtcc gctggatatc gttgtctatg gcctcggcca
     8761 gctcgaaggc gcaccccaac cgccccagaa gcatgaccag atgctcgact ggctgaaatc
     8821 gcttggtttc aagaccccgg accgcacctg gcactgccgc tcggcggatg aactggtcgc
     8881 cgccatcgaa gccctcgacc aggtgcggcg cgagttcgcc tacgagaccg acggggcggt
     8941 tatcaaactg aactccttcg cgcagcgcga gatagccggc tttacttcca aagcgccacg
     9001 ctggtccatt gcctataaat acgccgcgga gcaggcggag acgcggctca acggcatcac
     9061 cgtccaggtc ggccgcacag gcgcgctcac acccgtcgcg gaactcgaac cggttttcct
     9121 ggccggcagc accatcagcc gcgccacgct ccacaacgaa gactacgtcc gccaaaagga
     9181 catccgcatc ggcgacacgg tgaagatcga gaaggcaggc gaggtcatcc ctgccgtggt
     9241 ggatgtagtc ctcgcccggc ggacagggaa ggaagtctcc tttcgctttc ccaggcaatg
     9301 cccggaatgc ggctccaaag tctcccgcgc gcccggcatc gtcgagggcg acgaaggcgt
     9361 cgtttggcgc tgcctgaatc ccgattgccc ggcgcaggtt cgcgggcgcc tcgagcattg
     9421 gtgtgcccgc ggcgcaatgg acatcgaagg tggcggagaa gttctcgctc gccaactagt
     9481 ccagaacggc ctggtgcgcg acgtggcgga gctttattcg ctcaagctct ccgatgtcgc
     9541 cggcctcgaa cgcatgggag agaagtcggc ccggaatttc ctcgacggcg tcgaagccag
     9601 caagcagcgc gacctatggc gggtgctcta cggcctgggg attctccacg tcggcgcggg
     9661 ggtggccaag gccctcgggc gcggatttgc cacgctcgat gacgtatttg ccgccagcgt
     9721 ggaccagttg accgattgcg aagacatcgg cgatgtcatt gcccaaagct tggtccaatg
     9781 gcagggcgac tcccgaaacc agaaactaat cgagcgcctg cggaaggcgg ggctgaattc
     9841 caaatctgcc ctctaccaac cgaaagccaa ggcaggtccg ctgaccggca agacctttgt
     9901 cctcaccggc acgttgccga cactcaagcg cgaagaggcc gccgcgaaga tcgaggcggc
     9961 gggcggcaaa gtcagcggca gcgtcagcaa gaaaaccgac tacgtcgtcg ccggcgcgga
    10021 agccggttcc aagctggaca aggcgcaggc gctcggcgtg aagatcattg acgaggcgga
    10081 gttgctgcgg ctgtgcggcg cgtgacgacc tagcgtgacg tcgatgaatc aggcctgata
    10141 tcgaacagat gactcttgag ggattacaat tgcgggcagg tttttgttga aaagcaaggg
    10201 cgtttgcccg agttcgccaa cgcccagcgc caattcatga aggccctttg gcgcaaagga
    10261 ggtgcttc
//
LOCUS       DATCGS010000218         3032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_564544, whole genome shotgun sequence.
ACCESSION   DATCGS010000218 DATCGS010000000
VERSION     DATCGS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564544"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VIL39_04385"
     CDS             complement(<1..845)
                     /locus_tag="VIL39_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEY5480694.1"
                     /translation="MMAATYGYFPGCSLKGTGAAYEESLLTLFRLLDLSLQELKDWNC
                     CGATSYMSIDENSAFGLSARNLGIAHEAGHRDLVTPCSACYLVLRKTQDYAAKYPQIA
                     KSMEGSPAAAALDAVKIRHPMELLYNDVGMARIKSKMVRKWKGGPVACYYGCQLVRPF
                     AEADRAHDPTRMDELLRAVGVPTVDWALKTKCCGGSLTGTIHEVGVRLNYILLKEAAR
                     KGAKAIVTICPLCQYNLDAYQAEIRKETGEAFDMPVLYFTQILGWALGGDAKSLGLGR
                     AISGCG"
     gene            complement(842..1444)
                     /locus_tag="VIL39_04390"
     CDS             complement(842..1444)
                     /locus_tag="VIL39_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5480695.1"
                     /translation="MKKLKAFWLSSDMRRKMKFQREADLQWAQQVAERPGCEGLFSCL
                     QCGTCSGTCPLSIYMDFGPRKIISLVREGFREEALSSQTIWLCASCYSCAVHCPRQIH
                     ITDVMYSLKREAIQQKLYPKRFPIPVLAQEFYKMVRRYGRSSEFWLVLRMALRTNPFQ
                     LLTMSGTGWQLMRTGRLRLGQDKIENVKELQSQMAGREVR"
     gene            complement(1490..2353)
                     /locus_tag="VIL39_04395"
     CDS             complement(1490..2353)
                     /locus_tag="VIL39_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5480696.1"
                     /translation="MDKSSQEALKNLNRYGNSFGAAPRARPNWTRQLQFSIKDARQEP
                     VQYLWFVGDYASYHPRVAAATCAAARVFHRAGLDFGILYEGECNSGNDARLAGEEGLF
                     EALRDKNLRSFAKAHFDEVVTTDPHSFHVLKHEYRATNGARTVRHHTQLLSEMVLSGE
                     LPLRRKLRMTVTYHDPCYLGRYNAIFNAPRHVMAALGLDLVEMPQSRTYAYCCGAGGG
                     RIWMEAPPAFKENPALTRVREAASLKGVRTLVVVCPKDLVMFQGAIGTTGLGDRLEVK
                     ELSELVEQAMG"
     gene            complement(2684..2872)
                     /locus_tag="VIL39_04400"
     CDS             complement(2684..2872)
                     /locus_tag="VIL39_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480697.1"
                     /translation="MVSPWPPGTKATTSEADLMDADTLQGIEKIYEAKLTEDELVALH
                     KSAAAVKELVDVMAPKLA"
BASE COUNT          565 a          989 c          879 g          599 t
ORIGIN      
        1 ccgcaccccg aaatggcgcg gcctaatccc aatgacttgg catcgcctcc caaagcccag
       61 cccaggatct gggtgaagta caaaaccggc atgtcaaacg cttcgccggt ttcctttcga
      121 atctccgcct ggtaagcgtc caggttgtac tggcacaagg ggcagatggt cacgatggcc
      181 ttcgctcctt tgcgcgcggc ttccttcagg aggatgtaat tcagccgcac gcccacttca
      241 tggatggtgc ccgttagcga accgccgcag cacttggtct taagcgccca gtccaccgtc
      301 ggcaccccca cggctctcag cagttcatcc atgcgcgtgg ggtcgtgcgc gcggtcggcc
      361 tcggcgaaag gccgtaccaa ctggcatccg tagtagcagg ccactggccc gcccttccac
      421 ttgcggacca tcttcgactt gatccgtgcc atccccacat cgttatagag gagctccatg
      481 gggtggcgaa tcttcaccgc atcgagcgcc gcggcggcgg gcgatccctc catgctcttg
      541 gcgatttgcg ggtacttggc cgcgtaatcc tgtgtcttgc gaagaaccag gtagcaggcg
      601 ctacacggcg tgaccagatc gcggtggccg gcctcgtgcg caatccccag gtttctcgcc
      661 gacaacccaa acgccgagtt ctcgtcaatg gacatgtacg aggtggcgcc gcagcagttc
      721 cagtccttca gttcctgcag agacagatcc agcagccgaa acagggtcag caggctctcc
      781 tcgtaagcag cgccagtgcc cttcagcgag cagcccggga aataaccgta tgtcgcagcc
      841 atcatcgcac ctcccggccg gccatttggc tttgcagttc tttcacgttc tcgatcttgt
      901 cctgacccaa ccggagcctg cccgtccgca tcagttgcca gcccgtcccc gacatggtca
      961 gcaattggaa aggattggtg cggagcgcca tgcggagcac cagccagaac tccgagctgc
     1021 gcccgtaccg gcggaccatc ttgtagaact cctgcgccag cacgggaatc gggaagcgct
     1081 tgggatagag cttctgctgg atcgcctccc gcttcaggct gtacatcaca tccgtgatgt
     1141 ggatctgccg gggacagtga acggcgcacg aatagcacga tgcgcatagc cagatggtct
     1201 gggaactgag cgcttcctcc cggaacccct cccgtaccag ggagataatc ttgcgcggac
     1261 caaagtccat gtagatcgac aacgggcacg tccccgagca ggttccacac tgcaggcacg
     1321 agaacagccc ttcacacccc ggccgctcgg cgacctgttg cgcccactgg aggtccgcct
     1381 cccgctggaa cttcatcttg cgtcgcatat ctgagctaag ccagaatgcc ttcagtttct
     1441 tcatagatct cctcgtcttg gaaccggtcc tgcccgatgg aggcgccagt taccccatag
     1501 cttgctccac cagttccgat agttctttca cttcgagccg gtctcccagt ccggttgtcc
     1561 cgatggcgcc ctggaacatc accaggtcct tcgggcagac caccaccaaa gtccgaaccc
     1621 ctttgagcga cgccgcctcg cgcaccctgg tgagcgccgg attctccttg aaggccggcg
     1681 gggcttccat ccagatgcgg ccgcccccgg cgccgcagca ataggcgtag gtacggcttt
     1741 gcggcatctc gacgaggtcc aatcccaaag cggccatcac gtggcgggga gcgttgaaaa
     1801 tggcattgta gcgccccagg taacacggat cgtggtatgt caccgtcatc ctcagcttcc
     1861 gccgcaacgg cagttctccg ctcagcacca tctcgctgag cagttgcgtg tgatgccgga
     1921 ccgtgcgcgc gccgttggtg gcgcggtact cgtgtttcaa cacgtggaag ctgtgcggat
     1981 cggtagtgac gacctcgtcg aaatgcgctt ttgcgaacga tctcagattc ttgtcgcgca
     2041 gagcctcaaa cagcccctct tcgcccgcca ggcgggcatc gtttccggag ttgcactcgc
     2101 cttcgtagag aatcccgaag tcgagaccgg ctctgtgaaa aacgcgcgcc gccgcgcaag
     2161 tcgcggccgc cacccgcgga tggtaagacg cgtaatcgcc cacgaaccac aggtactgga
     2221 ccggctcctg gcgggcatcc ttgatggaga actgcagttg ccgagtccag ttcggacggg
     2281 cccgaggggc cgccccaaaa gaatttccgt aacggttcag gttcttcaac gcttcctggg
     2341 aagatttgtc catatcaccc ttcccaacgg gcgccggcgc gtccgatcgg cgcgcgggcg
     2401 tccagttgtc gtgctaaaag tgcgaaggaa gtcttgagga ataatgaatc ggtgagcaat
     2461 cgtgttcggc gtttctcgct cacagggtcc cacctaagag aataacacct tattctcttt
     2521 tgcccaacaa gtccccgcac aacgcgacgg gacggcctcc gcgatggcag gtagtgacgc
     2581 gattcacaac aatctgagaa tttcgtaggg ttgacatgcg gaagcgcctg tcccgccttg
     2641 gtgcgccctt gtgggcagac gcttccgtct gtcagcccgg tgctcaggcg agcttgggcg
     2701 ccatcacatc cacgagttcc ttgacggccg ccgcgctctt gtgcagcgcc acgagttcat
     2761 cttccgtcag cttggcttcg tagatcttct cgatgccctg gagggtgtcg gcatccatca
     2821 gatccgcctc ggacgtggtg gccttcgtgc cgggcggcca cggcgacacc atggcgcctt
     2881 tggtgcgcta cagcagcgtg gccggtatcc cgctggctga cggaagatga actcgtggcg
     2941 ctgcacaaga gcgcggcggc cgtcaaggaa ctcgtggatg tgatggcgcc caagctcgcc
     3001 tgagcaccgg gctgacagac ggaagcgtct gc
//
LOCUS       DATCGS010000219         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_364842, whole genome shotgun sequence.
ACCESSION   DATCGS010000219 DATCGS010000000
VERSION     DATCGS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364842"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VIL39_04405"
     CDS             complement(<1..413)
                     /locus_tag="VIL39_04405"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY5480698.1"
                     /translation="MHPIALHLGPLTIHWYGAMVALGFLAGLWTAGRRAMHAGIAAEK
                     IIDLGPWLIIGAILGARTLYVISYWHELFEGRPIAEVFMVWKGGLVFYGGLVGSSLAC
                     ILYTRIKKLPLWKVADILAPSVALGYVFGRIGCLL"
     gene            complement(523..1524)
                     /locus_tag="VIL39_04410"
     CDS             complement(523..1524)
                     /locus_tag="VIL39_04410"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY5480699.1"
                     /translation="MSLPPLSICRVAALLGASHLLLAALPAQAAATGAVSAPANSAPA
                     RPPMPSLPPALSPISFFRDLLAMDAAGREQALTNRPPQIKAQILAKVREYESLKPDER
                     ELRLRVTELRWYLRPLMSASATNRPAQLAMIPETYRKLVEDRLQEWDKLSPDLQAELL
                     VNESTIRYLTEIEGRTEEQRRQTLEAIPLGGRTVLERGIDKWSSLSEDRRQRMLSRFR
                     EFFELTAQEKRKVINTLPGLGRGQIEKTMRALDNLPPDQRDQCIRSLEKFAGLSLAER
                     QQFLKSAERWKLMSPSERQVWRELVQRFPPLPPPPLPPLPRSLRPTPPVLPIATNRN"
     gene            complement(1521..2021)
                     /locus_tag="VIL39_04415"
     CDS             complement(1521..2021)
                     /locus_tag="VIL39_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480700.1"
                     /translation="MTNDPIYNHLRELSWQRQLTGTETAQLQAWLAAHPEAQADWEAE
                     AGLNAALGRLPDVPVPSNFTARVLQAAEREAAAELRRSGWKWGGWPRLRWLPKLALTA
                     SVICAGVVSCLLVQDAQRKNLVESVAVVSAVSSLPGPDILKDFDAIRASNPTSAADEQ
                     LLAVLQ"
     gene            complement(2068..2676)
                     /locus_tag="VIL39_04420"
     CDS             complement(2068..2676)
                     /locus_tag="VIL39_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480701.1"
                     /translation="MAVESDPDAALMLCVKQGDMAAFAVLVDKYKQPVLNLASRMLRD
                     ATEAEDLAQAVFVQVFKSASRYQVASKFSTWLFTIARNLCLNEIRRRSRHPVDSMDTP
                     HPEQEDQPLHQFEDRKTASPPEHLLHGELEQKIEQALAGLPENQRLAILLCRQDELSY
                     EEIARVLGCSVSATKSLIHRGRETLKQRLKPYLQTGVWHESK"
     gene            2764..>3296
                     /locus_tag="VIL39_04425"
     CDS             2764..>3296
                     /locus_tag="VIL39_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY5480702.1"
                     /translation="MQELLKKIEASAAARLVLPPGRQPAQELARYKAFLKVETHRLKL
                     HHRAGTGGLEICHARAAILDALLRHLWEAAKAGLSEQGRKEFPQLALVAIGGYGRAEL
                     NPHSDIDIMFLHNRQVTAGARPLPHLSRIVDGLLYPLWDLGLKIGHSVRSVEDCVKVA
                     NSDMQSKTSLIEARHVTG"
BASE COUNT          630 a         1039 c         1030 g          597 t
ORIGIN      
        1 ttgagcaggc agccgatgcg gccgaagacg taacccaatg caacgctcgg cgcgagaatg
       61 tcggctacct tccagagcgg cagcttcttg atcctggtgt agagaatgca ggctagcgag
      121 gagccgacca gtcccccgta aaaaaccagc ccgcccttcc agaccatgaa gacctccgct
      181 atcggcctgc cttcaaatag ctcgtgccaa taggaaatga cgtaaagtgt tcgcgcgccg
      241 aggatcgcgc caataataag ccacgggccc aggtcaatga ttttctcggc ggcaatgccc
      301 gcgtgcatag ctcgccgacc ggcggtccac agaccggcca gaaagccgag cgcgaccatt
      361 gcgccgtacc agtgaatggt aagcgggccg agatgaagcg caatcggatg catttctcag
      421 agtgtaggat cgaaaggcat ggctggaaat cgaaattgcg ccctggcgtc catgcagtca
      481 aacccgggcc ggagcagcgc tggcctcggc caccgcacgc agtcagttcc ggttcgtggc
      541 tatggggagc accggtggag tcgggcgcaa cgatctcggc aggggcggca gcggcggcgg
      601 cgggagcgga ggaaagcgtt ggaccagttc gcgccagact tggcgttcgc tgggcgacat
      661 cagcttccag cgctcagcac tcttcaggaa ctgctgacgc tcggcaaggc tcagacccgc
      721 gaacttctcg agggatcgaa tgcactggtc acgctgatcg gggggcaaat tgtccaaggc
      781 ccgcatcgtt ttctcgatct ggccccggcc cagcccgggc agcgtattga taaccttccg
      841 tttttcctgg gcggtaagct cgaaaaactc tcggaagcgg cttaacatcc tctggcgccg
      901 gtcctcagac aacgagctcc acttatcaat gccccgttcc agcacggtgc gcccccccag
      961 cgggatggcc tccagcgtct gccgcctctg ctcctcggtg cggccttcga tttcggtcag
     1021 gtaacggatg gtggattcgt tcaccaaaag ctccgcttgc agatccggcg acagcttatc
     1081 ccactcctgc aaccggtctt cgaccaattt gcggtatgtc tccggaatca tggctagttg
     1141 ggcggggcga ttggttgccg acgcggacat cagcggccgc agataccagc gcagttcggt
     1201 gacccgcaga cgcagttcgc gctcgtccgg tttgagtgat tcatactcgc gcactttggc
     1261 caatatctgc gccttgatct gtggcggacg attcgtcaga gcctgttccc ggccggcagc
     1321 atccatcgcg agcaggtcgc ggaagaagct tatgggcgag agggccggtg gcaacgacgg
     1381 catgggcggg cgcgcgggag ccgagttggc tggcgccgaa accgcgccgg tggcagccgc
     1441 ctgagccggc agcgcggcca gaagcaggtg gctggcgcca agcagcgcgg ccacccggca
     1501 aatgctaagc gggggcaaac tcattgcagc accgcgagca attgctcgtc cgcggcagat
     1561 gtcgggttgg acgcgcgaat agcgtcaaag tctttgagaa tgtcagggcc aggcagagaa
     1621 gacaccgccg aaacgaccgc cacgctttcc accaggttct tgcgttgggc gtcctgaacc
     1681 agcaggcagg acaccactcc cgcgcatatt acgctggccg tcaaagccag cttgggcagc
     1741 cagcgcagcc gcggccagcc cccccatttc catccagatc ggcgcagttc cgccgcggct
     1801 tcccgttcag cggcctgcaa cacgcgtgcc gtgaaattgc tcggcacggg cacgtccggc
     1861 agccggccca gggccgcgtt tagcccggct tcggcttccc agtccgcctg cgcctcaggg
     1921 tgcgcggcca gccaggcctg caattgggcg gtttcggtgc cggtgagttg ccgctgccag
     1981 cttagctcgc gcaagtggtt atagatgggg tcgtttgtca tataacgctg ctagtagtat
     2041 taaaaccgtt gggttcacaa agttgcttca tttcgactcg tgccagacgc cggtctgcaa
     2101 gtagggcttc agcctctgct taagcgtctc ccggccccgg tgaatgaggg atttcgtcgc
     2161 agataccgaa cagccaagca cccgggcgat ttcctcgtag ctcagctcat cctggcggca
     2221 aagcaggatg gcgaggcgct ggttttccgg cagcccggcc agggcctgct cgatcttttg
     2281 ttccagctcg ccgtgcaaca ggtgctcggg cggcgaggct gttttcctgt cctcgaactg
     2341 gtgtagcggc tggtcttcct gctccgggtg gggggtgtcc atcgaatcaa ccggatgccg
     2401 ggagcggcgg cggatttcgt tcaggcacag gttccgcgca atggtgaaca gccaggtcga
     2461 gaacttggag gcaacctgat aacggctcgc ggatttgaac acctgcacaa agaccgcttg
     2521 cgcgaggtcc tcggcttccg tcgcgtcgcg caacatgcgg gaggcgaggt taaggaccgg
     2581 ctgcttgtac ttgtccacca gcacggcaaa agcagccata tcgccctgct tcacgcacaa
     2641 catcagggcg gcatcgggat cggactcaac agccatattt gccaatctaa caaacaccgc
     2701 cgcggccaca aggtttcgcg ggcgcttgcg ttaaggggct tttgaaccca aagtgattcg
     2761 cgaatgcagg aattgctgaa gaagatcgaa gcgagtgccg cggcgcgcct ggtgctgccg
     2821 ccaggccggc agcctgccca ggaattggcc cgctacaagg ccttcctgaa ggtggaaaca
     2881 caccggctca agctgcacca tcgcgccggc accggcggcc tcgaaatctg ccacgcccgc
     2941 gccgcgatcc tcgatgcgct gctgcgccac ctgtgggaag ccgccaaagc cggcttgtcc
     3001 gaacagggcc gcaaggagtt tccccaactg gcgctggtgg ccatcggcgg ctacggccgg
     3061 gcggagctta atccgcacag cgacattgat atcatgttcc tgcacaaccg gcaggtcacc
     3121 gccggcgccc gcccgctgcc gcacctgtcc cgcatcgttg acggcctcct ctatccgctg
     3181 tgggacctcg gcctgaagat cggacactcg gtccgcagcg tcgaggactg tgtcaaagtg
     3241 gccaatagcg acatgcaatc caagacctcg ctcatcgagg cccggcatgt cacggg
//
LOCUS       DATCGS010000220         6374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_664029, whole genome shotgun sequence.
ACCESSION   DATCGS010000220 DATCGS010000000
VERSION     DATCGS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664029"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VIL39_04430"
     CDS             <1..519
                     /locus_tag="VIL39_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5480703.1"
                     /translation="KSGKPYSMVTLEDLEGSVQVLCFNENYDKYRELLVPGKAILVIG
                     EVNVGDDKPKLFPQEIMPLEDAPGRFTKQVHLRLHTAHLKPEHLESVQELVAAHPGKC
                     PLFLCFVQPTGEVIFMEAHDKFSVAPSRALQQAADDQFGEETYYVKVDTSLPERAQRR
                     WERRTEGNGTEG"
     gene            complement(617..2050)
                     /locus_tag="VIL39_04435"
     CDS             complement(617..2050)
                     /locus_tag="VIL39_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY5480704.1"
                     /translation="MSNQQTQPVSQLPGYITSAVPNPRNNRAPWYKNTAPTYAGIFLW
                     FVFWQDAAKAGAPGGTLAQGVGWALLSLVISAFVCHFLFYLVPGLLGMKTGLPLYIVG
                     TSTFGAQGGFIMPGFLMGVLQFGWVGVNVYFSSQALSAMIPIDARIIMVVWGVLACFV
                     GLKGIQYVAKVATYLPLIPLVVLLWLTASTIGNVGSFSPDALITAQQAANPAGSGALS
                     MMGVLAAMLTYVVGFFATAGAAGVDFGLNSRDEKDVKMGGLIGIALAIIVTAGLSLLI
                     VAGTYGGIKAPLDPAAANKLSLSSFELIPTVLGPGTAKWVMFLLAVAAFPSACFSAFI
                     AANSFKTTLPKVNPFISVGIGTVVAIILAITGVVAKAISVFVIIGASFGPICGAMLVD
                     YLLSGKKWSGPRAGFNPAGWISWALGFLVGIMPNLHGWKPDLFPDMPAAPVLAFVVGA
                     VVYFLCAKMGMLSPVVRVSGPLAEATK"
     gene            2211..3758
                     /locus_tag="VIL39_04440"
     CDS             2211..3758
                     /locus_tag="VIL39_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY5480705.1"
                     /translation="MSLFQRVREATQALLDERGPAALADFSNWQKFAHFWVLVWKSFT
                     RNRCPVRASALAYATVLAIIPMLAVVMSITSGLLKKEGEDQIDQFIVKIVASVTSAAM
                     VDTNAAGTTTNPRVEESSVIGATNSATTSTNLAAETTNSLALSTGGSSTVTNGLPSFA
                     RDAEAVKARHAMARYIHEFIQNTRSGALGVTGSILLIFAAISMLSRIEDTFNDIWGVA
                     RGRGWFMRVVLYWGVISLAPILLIVALGLASGPHLEGTRKLIIAMPFVSSLAFRILPV
                     VVLCLTFAVFYALIPNTKVHWGAALAGGLAGGILFHLNNVVSVLYVSRVVSNSKIYGS
                     LGLVPVFMIGLYFSWLILLFGAQVAYAYQNRATYFEERQVENINQRGREFIALRLMTC
                     IGQRFTRGESPLSAVEIAEALTVPTRLVQQIMRTLSTARLVIETAGAEPGYLPARPLE
                     TITCHDILLALRASQGQELATRDEPTRSEVYGEFHRIEEAERHAAASVTMLALVNRAQ
                     AQKALTD"
     gene            3790..3927
                     /locus_tag="VIL39_04445"
     CDS             3790..3927
                     /locus_tag="VIL39_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480706.1"
                     /translation="MEYTKRRNLNRGYMKLEVWQRGMDLFELKRENHTWQDTLPQSNN
                     P"
     gene            3980..5080
                     /gene="ald"
                     /locus_tag="VIL39_04450"
     CDS             3980..5080
                     /gene="ald"
                     /locus_tag="VIL39_04450"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY5480707.1"
                     /translation="MIIGVPKEIKDQEYRVALLPSAAYQLIKRGHQVLVERNAGVGSG
                     YPDADYEQAGATMVSDHTAAFAKADIVVKVKEPLPSEYPLLKPGQLLFTYLHLAASRS
                     LTDALLKSRVTGLAYETIEVNHRLPLLEPMSEIAGRMSILVGGYFLAKHNGGSGVLLG
                     GVPGVLPGKVVVLGGGCSGINAARMAQGMGADVTILEVDLERMRFLDITLHTAHTLYS
                     NEAHLLELLPTVDLLIGAVLVPGAKAPRLIRRDMLRRMRPGSVLVDIAIDQGGCAETS
                     RPTTHDHPVFVEEGVTHYCVANMPGAYARTATQALANATYRYLELLADHGLAEACQMQ
                     PALVGGLNVMDGKITQKAVAGAHGLPYFPPAL"
     gene            5144..5839
                     /locus_tag="VIL39_04455"
     CDS             5144..5839
                     /locus_tag="VIL39_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5480708.1"
                     /translation="MRAPIPDNEAQRLAALQEYHILDTAAEQAYDDIVALAAYLCNVP
                     IALITLVDESRTWFKSRLGLNEKETPRDVAFCAHAILQTEPLIVRDALKDRRFSDNAL
                     VTGSPHIRFYAGFPLVNPDGFALGTLCVIDRKPRRLSAEQKTAMQALARQVMALLELR
                     RVSAQMAEALEKVKTLHGLLPICAWCKRIRDDKGYWSQVEAYIHAHTGADFTHGICPD
                     CFEKQRPRKGPGE"
     gene            complement(5891..6355)
                     /locus_tag="VIL39_04460"
     CDS             complement(5891..6355)
                     /locus_tag="VIL39_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480709.1"
                     /translation="MGPLQIEKATDENGKLGAWVALDPGAEPVFVAQHHWIGVDLDGT
                     LARDDAAGHFLPPYPLGEPIPEMIEMVKSLLAAGVTVKIFSARACEPESIPIIQAWAE
                     QHGLGRPEVTNQKDYDLIRYYDDRAIQMVPNRGKSVGCLANCKPGGGDGVME"
BASE COUNT         1313 a         1888 c         2003 g         1170 t
ORIGIN      
        1 aagagcggca agccgtattc gatggtaacg ctggaggact tggaaggctc ggtgcaggtg
       61 ctttgcttta acgagaacta cgacaagtat cgcgaactgc tggtgccagg caaggccatc
      121 ctggtcatcg gcgaagtcaa cgtcggagac gacaagccga agctctttcc ccaggagatt
      181 atgccgctgg aggacgcgcc gggacggttc accaagcagg tccacctgcg cctgcacaca
      241 gcacacctga aacccgagca cctcgaatcg gtccaggaac tggtggccgc gcacccgggc
      301 aagtgcccct tgttcctctg ttttgtgcaa ccgacgggcg aggtcatttt catggaggcg
      361 catgacaagt tctccgtcgc cccgtcgcgg gcgttgcagc aagcggccga cgaccagttc
      421 ggcgaggaga cctactacgt gaaggtggat acgagtttgc cggagcgagc acagcgccga
      481 tgggaacggc ggacggaggg gaatgggacg gaaggctaaa gtctaaagtc cagggtctaa
      541 aggccggagg gcggaaacaa gaacccgcct tggccacgaa tggcaaaggc gggttgaagg
      601 gaactaagcg cgcaggttac ttggtcgctt cggccagggg gccgctgacg cgcacgaccg
      661 gggagagcat ccccatcttc gcgcagagga aatacaccac cgcgccgaca acgaacgcta
      721 gcaccggagc ggcgggcatg tccgggaaca ggtcgggttt ccagccgtgc aggttgggca
      781 tgatgccgac caggaagcca agcgcccaag agatccaacc cgctgggttg aagcccgcgc
      841 gcgggccgct ccatttcttg ccgctgagca ggtagtccac cagcatcgcg ccgcagatcg
      901 gaccgaacga ggcgccgatg atcacaaaca ccgagatagc cttggccacc acgccggtaa
      961 tcgccaggat gatcgcaact accgtgccga tgccgaccga gataaaggga ttcacctttg
     1021 gcagcgtggt cttaaagctg ttggcggcga taaacgctga gaagcaagcg gacgggaagg
     1081 ccgccaccgc cagcaggaac atcacccact tggccgtgcc cgggccgagc acggtgggga
     1141 tgagttcaaa ggaactgagg ctgagcttgt tggccgcggc agggtcgagc ggggccttta
     1201 tgcccccata ggtgccggcc acgatcagca gcgacaggcc ggccgtcacg atgatggcca
     1261 gggcgatgcc gatcaggccg cccattttca catctttctc atcgcggctg ttcaggccga
     1321 agtccacgcc cgcagcgccc gccgtggcaa agaagccgac cacataagtc agcatcgccg
     1381 ccagcacgcc catcatgctt aacgcaccgg accccgcggg gttcgccgcc tgttgcgcgg
     1441 taatcaacgc gtcgggacta aagctgccga cattgccaat ggtcgaggcg gtcagccaaa
     1501 gcagcaccac cagcgggata aggggcaggt aagtggcgac tttggcgacg tactgaatgc
     1561 ctttcagtcc gacaaagcag gccagcacgc cccacaccac cataatgatg cgggcgtcga
     1621 tgggaatcat tgcgctcaag gcctgcgaag agaaatagac gttcacaccc acccaaccga
     1681 actgtaagac gcccatgagg aagccgggca tgatgaagcc gccttgtgcg ccgaaagtcg
     1741 aggtgccgac aatgtagagc ggcaggccgg tcttcatgcc gagcaaaccc ggcacgaggt
     1801 agaacaggaa atggcagacg aaggccgaga tgaccaggct gagcaacgcc cagcccaccc
     1861 cctgcgccag cgtcccgccc ggagcgccgg ctttggcagc gtcctgccaa aacacgaacc
     1921 acaggaagat gcccgcgtaa gttggggcgg tgttcttata ccagggggcg cggttgttcc
     1981 tgggattggg aaccgcgctg gtgatgtatc ctggaagttg gctaaccggt tgtgtttgtt
     2041 gattactcat attttagtta aggattcggt catgctgcct gactccgaac agacggcaag
     2101 cattgggggg gaatagtccc gctcgcgggg aaactgtcaa ccgtaaagtt ccggcaattg
     2161 acttggcttc gcgctctgtt agcgtgggcg gcgtcagtat ggcaaaaaag gtgtccctat
     2221 ttcaacgggt tcgcgaggcc acgcaggccc tgctggacga gcgggggcct gcggcgctgg
     2281 ccgacttctc gaactggcag aagttcgcgc atttctgggt gctggtgtgg aagagtttca
     2341 cccggaaccg gtgcccggtg cgcgcgtccg ccctggccta cgcgacggtg ctggcgatca
     2401 tcccgatgct ggctgtggtt atgagtatca ccagcggcct gctgaaaaag gaaggcgaag
     2461 accagatcga ccagttcatc gtgaagatcg tcgcgagcgt cacctcggca gcaatggttg
     2521 acacgaatgc cgccggcacg acgaccaacc cgagggtgga agagagttct gtaatcggtg
     2581 caaccaattc cgccaccacc tccaccaacc tcgctgctga aacgaccaac agccttgcgc
     2641 tttccaccgg cggcagctca accgttacca atgggctgcc ttcctttgcc cgggacgcgg
     2701 aagccgtgaa agcccggcac gccatggcac ggtacattca tgagttcatt cagaacaccc
     2761 gcagcggcgc gttgggggtg acgggcagca tcctgctgat ctttgcggcg atctcgatgc
     2821 tgagccggat cgaggacacc ttcaacgaca tctggggtgt ggcgcgcggg cggggctggt
     2881 tcatgcgcgt cgtgctgtat tggggcgtca tcagcctggc gccgatactg ttgatcgtgg
     2941 cgctggggct ggcctcggga ccccacttgg aaggaacgag aaagctgatc atcgccatgc
     3001 cgtttgtgag cagcctcgcc ttccgaatcc tgccggtggt ggtattgtgc ctaacgttcg
     3061 cggtgttcta cgccctgata ccgaacacca aggtccattg gggtgcggcg ctggccggcg
     3121 ggctggcagg gggcatcctg tttcacctga acaacgttgt gagcgtgctc tacgtgtcgc
     3181 gcgtggtgtc caacagcaag atctatggca gcctcgggct ggtgccggtg tttatgattg
     3241 gcctgtattt ctcgtggctg atcctgctct tcggcgcgca ggtggcctat gcgtatcaga
     3301 accgggcgac ctattttgag gagaggcagg tcgagaatat taatcagcgc gggcgcgagt
     3361 tcattgcgct gcggttgatg acctgcattg ggcagcgttt tacacgcggg gagtcgccgc
     3421 tcagcgcggt ggaaatcgcc gaggcgctta cggtgccaac ccgcctggtg cagcaaatca
     3481 tgcgaacgct ctccaccgct cggctggtaa tcgagactgc gggcgccgag ccgggttacc
     3541 tcccggcgcg tccgctggag accatcacct gccatgacat tctgctggcc ctgcgcgcca
     3601 gccagggcca agagttggcc acgcgcgacg agccgacgcg cagcgaagtc tatggcgagt
     3661 tccaccgtat cgaggaggcg gaacgccacg ccgccgcctc ggtcaccatg ctggcgctgg
     3721 tcaaccgggc gcaggctcaa aaggcattaa cggattaatg gattgatgga ttgatgaagg
     3781 gctgaaccta tggaatatac gaaacggcgc aatctgaatc ggggctacat gaagctggaa
     3841 gtgtggcagc ggggaatgga tttatttgag ttaaaacgcg agaatcacac ttggcaggac
     3901 accctccccc aatccaacaa tccatgaatc caggaatcca tttatccatt catccaccac
     3961 tccatcactc caatccgtta tgatcatcgg cgtccctaaa gaaatcaaag accaggaata
     4021 ccgagtggcc ttgttgccct cggcagctta tcaactcatc aaacgcggcc accaggtgtt
     4081 ggtcgagcgc aacgccggcg tcggctcggg ctatcccgat gccgactacg agcaggccgg
     4141 cgcgacgatg gtgagcgacc acacggcagc tttcgcgaag gctgacatcg tcgtgaaggt
     4201 caaggagccg ctgccatccg aatacccact gctcaagccg gggcaactcc tttttaccta
     4261 tcttcacctt gccgcgagcc gctcgctgac ggatgcgctg ctaaagtcgc gcgtcaccgg
     4321 cctggcctac gagacgattg aagtcaacca ccggctacca ttgctggagc cgatgagtga
     4381 aatcgcgggg cgcatgtcca tcctggtggg cggctatttt ctggccaaac acaacggcgg
     4441 cagcggggtc ttgctgggcg gcgtgccggg cgtgttgcca ggcaaggtcg tcgtgctggg
     4501 cggcggctgt tccggcatca acgccgcgcg catggcccag ggcatggggg cggatgtcac
     4561 catcctggag gtggacctgg aacggatgcg gttcctggac atcacgctgc acaccgccca
     4621 cacgctttac tcgaatgaag cgcatctgct ggagttgctg cccacggtgg atttgctcat
     4681 cggcgcggtc ctggtgccgg gagccaaagc gccccggctg attcgccgcg acatgctgcg
     4741 gcggatgcgg ccgggcagcg tgctggtgga tatcgcgatt gatcagggcg gctgcgccga
     4801 aacgtcgcgc ccgaccacgc acgaccaccc ggtgttcgtg gaggaaggcg ttactcacta
     4861 ttgcgtcgcc aacatgccgg gcgcttacgc gcggacggcg acacaggcgt tggcgaacgc
     4921 cacttaccgt tacctggagt tgctggcgga tcacgggctg gccgaagcct gccagatgca
     4981 gccggccctc gtaggcggcc tcaacgtgat ggatggcaag atcacgcaaa aggccgtggc
     5041 tggggcgcat gggctgcctt attttccacc agctctttga ggggtagtga cgcctcaggc
     5101 aagacgcccc agcaggtagc gcagcaagca cccaaagctc cgaatgagag ccccaatacc
     5161 ggacaatgag gcacagcggc tggccgcctt gcaggaatat cacatcctgg ataccgcggc
     5221 ggaacaggct tacgatgata tcgtcgcgct ggcggcatac ctttgtaacg tgcccatcgc
     5281 gctgatcacg ctggtggatg aatccaggac gtggttcaaa tcaaggctgg ggttgaatga
     5341 gaaagagacg cctcgcgacg tggctttttg tgcgcatgcg attctccaga cggaaccctt
     5401 gatcgtgcgg gatgcgctca aggataggcg gttttcggac aacgccctgg tcaccggttc
     5461 accgcatata cggttttatg ccgggtttcc gctggtcaac ccggatggct ttgctttagg
     5521 cacactctgc gtcattgatc gcaagccacg gcgactctcc gccgagcaaa agactgcgat
     5581 gcaggccctg gcacggcagg tcatggcgct gctggagttg cggcgcgttt cggcgcaaat
     5641 ggcggaggcg ttggagaagg tcaagacgct gcacgggctg ttgccgattt gcgcctggtg
     5701 caagcgcatc cgcgatgaca agggctactg gagccaggtc gaggcctata tccatgcaca
     5761 taccggggcc gactttaccc acggcatctg ccccgactgc ttcgaaaagc agcgccccag
     5821 aaaagggccg ggggagtgat ggggtgttgg agtgatggag tgatggcaaa ccctccactc
     5881 caatactcca tcactccatt actccatccc ctccgcctgg cttgcagtta gccagacaac
     5941 cgacggattt cccgcggttg ggcaccattt gaatcgcccg gtcgtcgtag taacgaatca
     6001 ggtcataatc cttctggttg gtgacttccg ggcgccccag cccgtgttgc tcagcccagg
     6061 cctgaataat cgggatgctc tccggttcac aagctctggc ggagaagatc ttaaccgtca
     6121 cacccgccgc caaaaggctc ttcaccatct caatcatctc cgggatgggc tcccccagcg
     6181 gatacggcgg aaggaaatgt cccgcggcat catcccgggc caaagtgccg tccaaatcca
     6241 caccaatcca gtggtgttgt gctacaaaaa cgggttcggc gccggggtcc agtgcgaccc
     6301 aggccccgag cttgccattc tcgtcagttg ctttctcgat ttgcagtggg cccataggga
     6361 gactctaaat tccc
//
LOCUS       DATCGS010000221         7725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_614125, whole genome shotgun sequence.
ACCESSION   DATCGS010000221 DATCGS010000000
VERSION     DATCGS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614125"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1211
                     /locus_tag="VIL39_04465"
     CDS             144..1211
                     /locus_tag="VIL39_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5480710.1"
                     /translation="MKTAVDGGASDVHVKMSTPIIFRINRQLVAIECPFPTEEWLNNV
                     VKQIIPAHLTKRVEQEREADFSYFVPGIGRFRTNLFQQRGQWCLAMRHVKTRVPSFED
                     LGLLEQIKKIAEAPRGIVLLAGSTGCGKSTTLAAMIEHINANFKKHLVTMEDPIEYVF
                     EDNQCVIEQREVGLDTLSFHHALKHVLRQDPDIIMIGEMRDSISFTSAMSAADTGHLV
                     LSTLHTTNASQSVSRILDFFKADEREQIRRQLSGTLQAVVCQRMVATVAGSMTPALEI
                     MINTPTVKKLIEENRLDKLAAAIETGVEDGMLNFNQALFNLVKQGKVSEQEALSKATN
                     PQALEMNFKGIFLDEGRRILS"
     gene            complement(1336..2700)
                     /locus_tag="VIL39_04470"
     CDS             complement(1336..2700)
                     /locus_tag="VIL39_04470"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY5480711.1"
                     /translation="MIGKTGVHTLYNILFAICLVLSAPYYFLRMWRRGNWQWGFTERF
                     GKYDTGLKQAITNRQTLWMHAVSVGEVNVCTQLIRALEPRLPNLKFVVSTTTTTGMGE
                     LRRKLPNHISKIYYPIDRRHYVTRALNTIRPEAIVLVEAEIWPNFLWRARALRMPVFL
                     VNARLSDRSYRGYKRFGFLFRPLFGSLTGVGAQNEADAAKLRTLGCRPEAIHIVGSLK
                     FDAAKLDERHLLDVPALLRQAGVVPGARLLVGGSTHAGEEAILAEQFLRLRSRFPDLF
                     LVLVPRHFERSREVGRELAARGLKFTYRSEIMSHTQFEPGELDCLIVNTTGELKYFYE
                     HATIIFIGKSLAAQGGQNPIEPGALGKAMVFGPNMQNFAPVVRSFVEQNGAVQVRDAA
                     ELEKVLGELLADESRREQLGRNALKVVHENLGAIDRTVDMILKHLAAGGLYVVPSKGP
                     GLDA"
     gene            complement(2764..4194)
                     /locus_tag="VIL39_04475"
     CDS             complement(2764..4194)
                     /locus_tag="VIL39_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY5480712.1"
                     /translation="MGYQLVEPNPWMILPFGLLLGAIALAPLFFPDWWARHYPKVAYS
                     LGAVTLIYYVFSLHAYQRTLHVGHEYLSFIALIGSLFVVSGGIHIKVKGEATPCVNTL
                     YLFLGALLANLLGTTGASMLLIRPWIQMNRCRITGYHIVFFIFIVSNVGGCLTPVGDP
                     PLFLGYLKGIPFWWVAEHCWPMWAVGVGFLLALFYFMDVANYRRTPREIRTRLAEAPE
                     QWRFDGLWNLAFLAVILGAVFLNHPPFAREALMVGAAVGSWLTTRKEVHEANQFNFHP
                     IREVAILFIGIFATMIPALDWLQNNASRVGQPTPALFYWSSGFLSSLLDNAPTYLSFL
                     SAAFGLFVDPAMVDKVQLLIQNHSADVAAVAGSHAEPIRHTIAALQEYHAAALASGQV
                     GTEQIEVAFLLGNGNLNQYILAISIGAVFFGASTYIGNGPNFMVKAIADHQRIRTPGF
                     LGYVFKYTLPCLAPMLVLIWLLFFRS"
     gene            4325..6268
                     /locus_tag="VIL39_04480"
     CDS             4325..6268
                     /locus_tag="VIL39_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5480713.1"
                     /translation="MTTTLFSELGLSSELLKAIDKLGFEQAAPIQAAAIPVLMQGKDV
                     YGQSQTGSGKTVAFAAPAIEKVVVEERTTQVLILCPTRELAVQVSEEVHKLALFKRGL
                     HALPVYGGQSYDRQLFGLRQGAHIVIGTPGRVMDHMRRGTLQLNTVKMVVLDEVDVML
                     NMGFRDDIELILQGTPKERQTVFFSATIPRPIQQLIEKYSRNPQSVRIEQKAMTVPTV
                     EQVFYECDRRYKVELLTRLIDIHDFKLGLIFCNTRRMVDELVEHLNAQGYSADRLHGE
                     MTQAMRDRVMNKFRKSGLEFLVATDVAARGIDVENIEVVFNYDLPYDGEDYVHRIGRT
                     GRQGRSGRAISFASGREVFQIRAIERYANTRIHRATPPTVSEVEAARADAVLDKIRAT
                     LKSGEFKRQDQLVERLLEEGFNSTDIASALLHHLQGGESAPAAKVAREEARHVREPLG
                     AAAPRPRVHTASRGPEQSGAGRPAYPQAGRPRYEKPAHTAREGRYDRYAERPPRNPDE
                     PAPRRERSPEPKPPGVVGRAPVKESPSPAQPPPQAAAPPMPEAPQRTEPAPVQPPKGS
                     RRTPDAQTRLYLNAGAEMGISEADIVGTILGETGLPRNVVGAVDIRERHLFVDVASEH
                     ANSIISKLNRTQIKGRKAKAKVA"
     gene            6268..6600
                     /locus_tag="VIL39_04485"
     CDS             6268..6600
                     /locus_tag="VIL39_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480714.1"
                     /translation="MKEQGSAKPVERLFPFVMRSRNLLIGRDTLRRSKSRLHFVLITR
                     DISEQSRAEVLSDFAYYPVVQHYTSAELEQFFGVKGTKVIGFQKSGLAQSIYAELKQH
                     RLNKPVKS"
     gene            complement(7015..>7725)
                     /locus_tag="VIL39_04490"
     CDS             complement(7015..>7725)
                     /locus_tag="VIL39_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5480715.1"
                     /translation="SEGDLSALSILADFLSGQLKARDRSVATKSLPPAYRRLQPLPVH
                     RLDQYTSGVFCMATNPAARHHLIEQLKAHTMKREYVAFVEGRASTPKGVWRQWLQLSR
                     DELRQHVISETQARVAASEAREAITHYEVIAEYPIAGGTRFVTKLRLWLETGRKHQIR
                     VQAANAGLPLLGDRTYNPGVRGPDATKTSIDFPRQALHAEVLTLEHPDQPGTRMSWTA
                     ELPKDLRQLESVLRLARR"
BASE COUNT         1654 a         2259 c         2392 g         1420 t
ORIGIN      
        1 acagttaccg gaaaggagag ccttcgaact ggagcttgat gcagccttgg ggtgacgtga
       61 tgacctgacg ttgtcatctt ttcgactttg gttcctcggt cccatttgtt accctgccga
      121 ctatggaaat atttcagcgt attttgaaga cggcggtcga tggcggagcc tcggatgttc
      181 acgtgaagat gagcactccc atcatcttcc gcatcaaccg tcaacttgtc gccatcgaat
      241 gtccttttcc gaccgaggaa tggctgaaca atgtggtgaa gcagattatc ccggcccatc
      301 tgaccaaacg cgtggagcag gagcgcgaag cggacttctc ttacttcgtg cccggcatcg
      361 gccggtttcg caccaatctg ttccaacaac gtggccagtg gtgcctggcc atgcgccatg
      421 tgaaaacccg cgtgcccagc ttcgaggacc tggggctgct ggagcaaatt aagaagatcg
      481 cggaagcgcc gcgcggtatc gtgctgctgg ccggttcaac cggttgcggc aaatcaacca
      541 cgctcgcggc gatgatcgag cacatcaacg ccaatttcaa gaagcacctc gtcacgatgg
      601 aagacccgat cgaatacgtg tttgaggaca atcaatgcgt catcgagcag cgtgaagtcg
      661 gtttggacac actctcattc catcacgcac tcaagcacgt cctgcggcag gaccccgaca
      721 tcatcatgat tggcgaaatg cgtgattcga tcagtttcac ctcggccatg agcgcggctg
      781 acaccggcca cctggtgcta tcgacgctgc acaccaccaa cgcctcccaa tcggtcagcc
      841 gtattctgga ctttttcaag gccgacgaac gcgagcagat tcgccgccag ctctcgggca
      901 ccctgcaagc ggtcgtttgc cagcgcatgg tggctaccgt ggccggcagc atgacgcccg
      961 cgctggagat catgatcaac acgcccacgg tgaaaaagct aatcgaagag aatcggctcg
     1021 acaaactcgc ggctgcgatc gagaccggcg ttgaggatgg catgctcaac ttcaatcaag
     1081 cgctgttcaa tctcgtgaag caagggaaag tcagcgagca ggaagccctg agcaaggcca
     1141 ccaacccgca agcgctcgag atgaacttca aaggcatatt cctggatgaa gggcggcgga
     1201 tcttgtccta aagcgaataa cgccacatgc gtggagcggc gaagccgcaa tcgagcaccc
     1261 tacgaacggg gggagttgga ctattgcacg gcaggtggtg gggaaattcc ccgcctctgc
     1321 ctttggtctc aaggatcacg catcaagtcc gggacccttg ctgggcacaa cataaagtcc
     1381 gccggcggcg agatgcttga gaatcatatc aaccgttcgg tcaatggcgc cgaggttttc
     1441 gtgaacgacc ttcagcgcgt tccggcctaa ttgttcgcgg cgcgactcat ccgccagcag
     1501 ttcgcccagg actttctcca gttctgccgc gtcccgcact tgcaccgcgc cgttctgctc
     1561 cacgaagctg cggacgactg gcgcaaagtt ctgcatgttg ggaccaaaaa ccatagcctt
     1621 gcccagggcg cccggttcaa tggggttctg gcccccttga gccgcaaggc tcttgccaat
     1681 gaagataatg gtggcgtgtt cgtagaagta tttcagttcg ccggtggtat tgacaatcag
     1741 gcaatcgagt tcccccggct cgaactgcgt gtgggacatg atctcgctgc gataggtgaa
     1801 cttcagaccg cgagccgcca gttcgcggcc aacctcccgg ctgcgctcga aatggcgcgg
     1861 aaccagcacg agaaacaggt ccggaaagcg cgagcgcaag cgaaggaatt gttcggcgag
     1921 aatagcctct tcacccgcat gggtgctgcc gccgacgagc aaccgtgcgc ccggcaccac
     1981 gcccgcctgg cgcaacaggg ctgggacatc cagcaaatgg cgctcgtcga gtttggccgc
     2041 gtcgaatttc aagctgccca cgatgtggat cgcctccggc cggcaaccca gggtgcggag
     2101 tttggcggca tcagcctcgt tttgcgctcc caccccagtc agcgagccga acagcggtct
     2161 gaacagaaag ccgaagcgct tgtagccgcg ataggagcgg tcggacaaac gcgcgttcac
     2221 caggaagacc ggcatccgca gggcgcgggc gcgccagagg aagttcggcc agatttccgc
     2281 ctccaccagc acgattgctt cggggcggat ggtgttgagg gcgcgggtga cataatggcg
     2341 gcggtcgatc ggatagtaga tcttggagat gtggttcggc agcttgcgcc gtaattcacc
     2401 catgcccgtc gtggtggtgg tggagacgac aaacttgagg ttcggcaagc gcggttcgag
     2461 cgcgcgaatg agttgggtgc agacgttgac ctcgccgacg cttacagcgt gcatccaaag
     2521 cgtttgacga ttggtgatgg cctgcttaag tccggtgtcg tatttgccaa accgctcggt
     2581 gaaaccccac tgccaattgc cacgccgcca cattcgcagg aagtagtacg gcgcggacaa
     2641 gacaaggcaa atcgcaaaca ggatgttgta aagggtgtgc actccggtct tccctatcat
     2701 caaattacac tcgattctgg gaagtttccc aaaaccgcgc aaggtaattc ttcgccctga
     2761 gcttcaactg cggaagaaga gcagccagat tagcaccagc atcggagcca ggcagggcag
     2821 ggtgtacttg aagacgtaac ctaagaagcc gggagtgcgg attctctggt ggtcggcgat
     2881 ggccttgacc atgaaattgg ggccgttgcc aatgtaagtg ctggcaccga aaaacaccgc
     2941 gccaatgctg atggcaagga tgtattggtt caaattgcca ttgccgagca ggaaggcgac
     3001 ttcgatttgc tcggtgccca cttggccgct ggcgagggcg gcggcatgat attcttgcag
     3061 cgctgcaatg gtgtgacgaa tcggttcggc gtgcgacccg gcaacggcgg caacgtccgc
     3121 gctgtggttt tggatcagca gttgcacttt gtccaccatt gcgggatcca cgaacaatcc
     3181 gaacgccgca ctcagaaagc tcaaataggt gggcgcgttg tccagcaggc tggaaaggaa
     3241 tccgctactc caatagaaca gggccggcgt gggttgaccc acccgggacg cgttgttctg
     3301 caaccagtcc agcgccggga tcatggtcgc gaagatgcca atgaacaaga tggccacttc
     3361 ccggatcgga tgaaagttga actggttcgc ctcatgcact tccttccggg tggtgagcca
     3421 ggagcccacc gccgcaccca ccatcaacgc ctcgcgcgca aagggcggat gattgaggaa
     3481 gaccgcgccc aatatgacgg ccagaaaggc gaggttccac aaaccgtcaa accgccattg
     3541 ttcgggagct tccgccagcc gcgtccgaat ttcccggggc gtccgccggt aattggccac
     3601 gtccatgaag tagaacaacg ccagcaggaa gcccacgccg accgcccaca tcggccagca
     3661 atgctcggcg acccaccaga acggaatgcc tttcaagtaa cccaggaaca gcggcgggtc
     3721 gcccacgggc gtcaggcagc ctcccacatt cgagacgata aagataaaga aaacgatgtg
     3781 atagccggtg atgcggcacc ggttcatttg gatccagggc cgaatgagca gcatcgaggc
     3841 gccggtggtg cccaacaggt tcgccagcag ggcgccaagg aacaggtaca gcgtgttgac
     3901 gcacggtgtc gcctcccctt tcaccttaat gtgaatgccg cctgagacga cgaacaatga
     3961 gccaatgagt gcgatgaagc tgagatactc gtgtcccaca tgaagggtgc gctgatacgc
     4021 atggagactg aacacgtaat agatgagcgt cacggcaccg agcgaatacg ccactttggg
     4081 ataatgcctg gcccaccaat ccgggaagaa tagcggcgcc agcgcaatgg cgcccagcag
     4141 cagcccaaag ggtaaaatca tccagggatt cggctcgacc aattggtaac ccatcccgcg
     4201 taccctatcg gcccgcgcca gatctggtca accagatttg acccgtcccg tgatcgtcgc
     4261 agaaactcct ctactttcac gcccggctga gctatgttgt gctttcgggg aactatttat
     4321 ttccgtgacg acaacattat tttctgaact ggggctttcg agcgaactac tgaaagccat
     4381 cgacaagctt ggtttcgagc aggcggcgcc gattcaagcc gcagcgatcc ctgtcctgat
     4441 gcaaggcaag gatgtgtatg ggcaatcgca gaccggttcc gggaagaccg tggccttcgc
     4501 cgcgcccgcc atcgaaaagg tggtggtgga agagcgcacc acgcaggtgc tgatcctttg
     4561 cccgacgcgt gaactggccg tgcaggtttc ggaggaggtc cacaagctgg cgctcttcaa
     4621 acggggcctc cacgccttgc cggtctatgg cggccaatcc tacgaccgcc agctcttcgg
     4681 gctgcgccag ggcgcgcaca ttgtcatcgg cacgccgggc cgggtcatgg accacatgcg
     4741 ccgcggcacg ctccagctca acacggtgaa gatggtcgtg ctcgatgagg tggatgtcat
     4801 gctcaacatg ggtttccgcg acgacatcga gctgattctt caggggactc ccaaggagcg
     4861 gcagacggtc ttcttctcgg cgaccatccc gcgccccatc cagcagttga tcgagaaata
     4921 ctcgcgcaac ccgcagagcg tgcgcatcga gcagaaggcg atgacggtgc cgaccgtcga
     4981 gcaggtcttc tacgagtgtg accgccgcta caaggtggag ttgctgacgc gcctcattga
     5041 catccacgat ttcaagctcg gcctcatctt ctgcaacacg cggcgcatgg tggatgagct
     5101 ggtggagcat ttgaatgcgc agggctactc cgcggaccgt ctccatgggg aaatgaccca
     5161 ggcgatgcgc gaccgggtga tgaacaagtt ccgcaagtcc ggcctcgaat tcctcgtcgc
     5221 caccgacgtg gcggcgcgcg gcattgacgt ggagaacatc gaggtcgtgt tcaactacga
     5281 tttgccttat gatggagagg attacgtgca tcgcatcggg cggacaggcc gccaagggcg
     5341 cagcggacgg gcgatctcat tcgcctcggg ccgtgaggtg tttcagattc gcgccatcga
     5401 gcgctatgcg aacacgagga ttcatcgcgc caccccgccc actgtcagtg aagtggaagc
     5461 ggcgcgcgcc gatgcagtgc tcgataagat acgcgccacg ctcaagagcg gcgaattcaa
     5521 acggcaggat caactcgtcg agcgtttgct cgaagaaggc ttcaattcga ccgacattgc
     5581 ttccgcgctg ctgcatcatt tgcagggcgg agaaagcgcg cccgcggcca aagtcgcgcg
     5641 cgaggaagcg cggcacgtgc gcgagccgtt gggagcggcg gcgccccgcc cgcgggtcca
     5701 cacggcgtct cgggggccgg agcagagcgg ggcgggacgc cccgcatacc cgcaggcggg
     5761 acgcccgcgc tacgagaaac ccgcgcatac agcccgggaa ggacggtacg accgctatgc
     5821 ggaacggcca ccacgtaatc ccgacgagcc cgccccccgg cgggagcgtt ctcctgagcc
     5881 caaaccgcct ggagtcgtcg ggcgtgcacc ggtcaaggaa agccccagcc cagcccaacc
     5941 cccgcctcaa gcagcggccc ccccaatgcc ggaagccccc cagcgcaccg aaccggcccc
     6001 ggtccaacct ccgaaaggca gccgccggac gccggatgcg caaacacgcc tttaccttaa
     6061 tgcaggcgcg gagatgggca tttcggaagc cgacatcgtc ggcacgattc tcggtgagac
     6121 gggtctgccc cggaacgtcg tcggcgcggt ggacatccgg gagcggcatc tgtttgttga
     6181 tgtggcttcc gaacatgcca acagcatcat ctcgaagctg aaccgcacgc agatcaaagg
     6241 ccgcaaagcc aaggcgaaag tggcgtaatg aaagagcaag gttcagccaa gccggtggaa
     6301 cggctgttcc cgtttgtcat gcggtcgcgc aatctgctga tcggacgcga cacgctgcgg
     6361 cgcagcaaga gcaggttgca cttcgtgctg atcacgcgcg acatatcgga gcagagccgc
     6421 gccgaggtgc tgtcggactt cgcttactac ccggtcgttc aacactacac ctcggcggaa
     6481 ctggagcagt ttttcggggt gaaaggaaca aaggtgattg gctttcagaa gtcgggtctg
     6541 gcacaatcca tttacgccga gctcaagcag catcggctca acaaaccggt gaagtcgtga
     6601 gcaggtttgg ttcgcctgct gcatgccggc tgtcatttag tacgagcgtc cggcgagatt
     6661 tgatggtaag ttatgaacac gaaaagtatc ttcaatgcag gaaatggcgg ccgtggccgg
     6721 atggcaccgg gcgcccggaa aggaccgaaa ggtcgaaaag ggggtcataa aggcagcggt
     6781 gggagaggac tgagaaccga accgctaatc gagcagccga ttgcagagtc gtttggcact
     6841 gtgccctgcc agatctgcgg ccacccggtt gatcccaagc gcatgcaccc tcacatggtg
     6901 cgatttcatg gcgcggcgat ccgatcgaga ggcgcgtggg aggataagtc taattcaacc
     6961 acaggttaag gcgaatagcc agtgatggga acgtgcgggg ggagcgggct cgggttagcg
     7021 ccgagccaag cgcaggacgc tttcgagctg gcgaaggtct ttgggcaatt ccgcagtcca
     7081 gctcatgcgc gtgcctggct ggtccggatg ttcaagggtg aggacttctg catgcaacgc
     7141 ctggcgggga aaatcaatgc tcgtctttgt ggcgtccggc ccgcgaactc ccgggttgta
     7201 agtgcggtcg ccgagcaggg ggagtccggc attcgcagcc tggacgcgaa tctgatgttt
     7261 ccggccggtt tccagccaga gccgcaattt ggtgacgaag cgtgtgccgc ccgcaatggg
     7321 gtattcagcg atgacttcgt agtgggtgat ggcctcgcgg gcctcggagg cggcgacccg
     7381 ggcttgcgtt tcggaaatga catgctgtcg cagctcatcc cggctcaatt gcagccattg
     7441 ccgccaaacc cctttaggcg tgctggcccg gccttccacg aaggccacat attcccgctt
     7501 catggtgtgc gctttgagct gctcgataag gtggtggcgc gcagcggggt tcgtggccat
     7561 gcagaagacg ccgctggtat attggtccag gcgatgcacc ggcaagggtt gcagccggcg
     7621 ataggcgggc ggcagtgact ttgtggcgac gctgcggtcg cgggctttaa gctggcccga
     7681 caagaagtcg gcgaggatgc tcagggcgga gaggtcgcct tcgga
//
LOCUS       DATCGS010000222         5123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_466142, whole genome shotgun sequence.
ACCESSION   DATCGS010000222 DATCGS010000000
VERSION     DATCGS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466142"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VIL39_04495"
     CDS             complement(<1..1336)
                     /locus_tag="VIL39_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5480716.1"
                     /translation="MRGCIDGRLLASLALCLLAPGLTFGQTNTNPITPHMVVDAEKLM
                     GLDFSEAKINLMLADLKEQASSFEALHRLAFSNDVPPALLFNPIPVGMKFETQRRKFK
                     LSPAGKVRLPANLDDLAFYSVGELAALIKTRQITSARLTRFYLERLKKYGPKLECVVT
                     LTEDLALQQARRADAEIAAGKYRGPLHGIPYGAKDLLAVKGIRTTWGAAPYTNQVFDY
                     DATVIKRLEAAGAVLVAKTTLGELAMGDVWFGGMTRNPWDLKGGSSGSSAGSTAATAA
                     GLIPFGIGSETYGSIVSPCDRCGATGLRPTYGRVSRVGAMPLSWSMDKIGPICRTVED
                     CAIVFNAIYGPDGLDQSVYDVPFNYDPKTKLSKLRIGYLKGDFDQEKGERKEHDEATL
                     QKMRALGANLIPITLPDYPLRNISFVLSTEAATAFDELTRSNRDDLLKQQGAD"
     gene            complement(1390..1890)
                     /gene="leuD"
                     /locus_tag="VIL39_04500"
     CDS             complement(1390..1890)
                     /gene="leuD"
                     /locus_tag="VIL39_04500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY5480717.1"
                     /translation="MPNIVFKVGDDVSTDVIYPGRYMATVLPAETPQFAFADETAFNA
                     KLKAKQVPHGSVLVGGKNFGCGSSREQAVSCLKGHELIIVANDFARIFLQNGINLGLR
                     MIVCPGIEAAEGDELDLTPEAVVNKTSGKQFKVEPMPKSRQVIIDAGGLIPYTRKRLL
                     EKTAAA"
     gene            complement(1992..3278)
                     /locus_tag="VIL39_04505"
     CDS             complement(1992..3278)
                     /locus_tag="VIL39_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY5480718.1"
                     /translation="MSMTVAEKILARAAGLPSVKAGDVVEPKVDLAMSHENAALVINQ
                     FLEIYKGTPLPSRIWDPSRVAVIFDHRVPAESPKTASNQKKVREFIGAQGIGRFHDIR
                     GDVGGICHQILPENGYVRPGYVVVGTDSHTTSHGALGALGFGIGATEMASVWALGSVL
                     NVEVPATIKVVVTGRFKKFVGPKDLILHLVGKISAQGANFKVLEFHGETIRNMSTSGR
                     LVLCNMAVEAGATAGIVPADEETLRYLREEAGVTDPIEPVLPDADAAYESVIEIKVSK
                     LQPQIACPHTVDNVKPIDQVAGKKVNQIVIGSCTNGRLDDLAIAAKIMKGQKVAQGTR
                     MLIFPASAKIYRQALGKGYVGVFMQAGAVVMNSGCGPCLGIHEGALGDGEVALSTTNR
                     NFKGRMGNPNSEVYLCSPAVAAASAITGVITDPRRK"
     gene            complement(3326..4552)
                     /locus_tag="VIL39_04510"
     CDS             complement(3326..4552)
                     /locus_tag="VIL39_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5480719.1"
                     /translation="MSNSQEKEQKPRKALQGVRVLDMTNVLAGPFATLHLALLGGEVI
                     KIENPKDGDLARKLGNVPKLNQQLMGTSFLAQNSNKKSLTLDLKSPEGKEIFKKLVAT
                     ADVLVENFRPDVMKRLGLDYPVLAQINPRLIYCAISGFGQTGPDAFKPAYDQIIQGLS
                     GVMAINGDERLNPLRAGFPVCDTVGGLNAAFAVLAALFHRERTGEGQFIDVALLDSIM
                     PLAGWVAANLLIGKQQPVLMGNDNFTAAPSGTFVTKDGYINIAANKQEQWEAVADLLG
                     MPELKTDPRFQDRDSRKKNRKALTPLLEVKLKEKETAYWIELLNRNDVPSGAILGLEA
                     ALNQPQVRHRQTLQPVSVEGVGELQLFGLSAKLEKTPGGIETPPPRLSAHTNEILGGL
                     GYSADDIKRLKEAHVI"
     gene            complement(4555..>5123)
                     /locus_tag="VIL39_04515"
     CDS             complement(4555..>5123)
                     /locus_tag="VIL39_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY5480720.1"
                     /translation="ADATKRIIAMARLATSAGKRVQLSVQSAFGCGFEGMVAPERVLK
                     IAEQFLNAGFRSISLADTAGHATPRQVEELFGGILKLAPDAECTCHFHNTYGLGMANC
                     CAAMDVGVRCFESSVAGLGGCPFTKVAGGNVCTEDLVYYLHRLGQRADVNQEKLVEIA
                     RDMSRFFNREMPGMVYKIGLAAATPACS"
BASE COUNT          943 a         1630 c         1526 g         1024 t
ORIGIN      
        1 catccgcgcc ctgctgtttc agcaggtcat cccggttgct gcgagtcagc tcgtcaaacg
       61 ccgtcgcggc ctccgtagaa agcacgaagg agatattccg cagcgggtag tcgggcagcg
      121 taatgggtat caaattcgcg cccagcgccc gcatcttctg aagcgtggcc tcgtcgtgct
      181 ctttgcgctc gcctttctcc tggtcgaagt cgcctttcag atagccgatg cgcagcttgc
      241 tcagcttggt cttaggatcg tagttgaaag gcacgtcata aacgctctgg tccaggccgt
      301 ccggcccgta aatcgcattg aacacaattg cgcagtcctc gaccgtgcgg cagatcggcc
      361 cgatcttgtc cattgaccag ctcagcggca ttgcgcctac acggctgacg cggccataag
      421 tgggccgcag acccgtcgcg ccgcagcggt cacagggcga cacgatcgac ccataagtct
      481 cggagccaat gccaaagggg atgaggcccg ctgcggtcgc cgccgttgat ccggccgacg
      541 agccgctgga accgcccttc aaatcccacg ggttgcgcgt catgccgccg aaccacacat
      601 cgcccatcgc cagttcgccc agggtcgtct tcgccaccaa taccgcgcct gctgcctcca
      661 accgtttgat cactgtcgca tcgtaatcaa agacttggtt ggtgtaaggc gccgcgcccc
      721 aagtggtccg gatgcctttg accgccagca aatccttcgc gccgtaggga ataccgtgca
      781 acggcccgcg atatttgccc gcggcgatct cggcgtccgc tcgtctggcc tgttgcagcg
      841 ccaggtcctc ggtgagcgtc accacgcact ccagcttcgg cccgtatttc ttcagccgct
      901 ccaggtaaaa gcgcgtcagc ctcgccgagg tgatttggcg cgtcttgatc agtgccgcca
      961 gctcgcccac tgaatagaag gccaggtcgt ccaggttcgc cggcagcctc actttgcccg
     1021 ccgggctcag cttgaacttc cgacgctgcg tctcgaactt catccccacc gggatgggat
     1081 tgaacaggag ggccggggga acgtcgttcg agaacgcaag tcggtggagc gcctcgaaac
     1141 tgctcgcctg ctccttcagg tccgccagca tcaagttgat cttggcctcg gagaagtcga
     1201 ggcccatcag cttctcggca tcaaccacca tgtgcggcgt aatggggttg gtattggtct
     1261 ggccaaaggt caggcccggg gccaagaggc agagagccag cgacgcgagc agcctcccgt
     1321 caatgcaccc gcgcattgca acaaagcagc gtagcagccg acgtaaggag gctctgatca
     1381 atttcgcatt caagcagcgg cagtcttttc gagcagccgc ttccgggtgt aaggaatcag
     1441 cccgcccgcg tcaatgatga cctgccggga cttgggcatt ggctcaacct tgaattgctt
     1501 gccgctggtt ttgttgacga ccgcctccgg ggtcaggtcc agctcgtcgc cttcagccgc
     1561 ttcgatgccg gggcagacga tcatgcgcaa gccgaggttg atgccgttct gtaggaagat
     1621 tcgggcaaag tcattcgcca caataatcag ctcgtgtccc ttcagacagg acaccgcctg
     1681 ctcgcgcgag gagccgcagc cgaagttctt cccgccgacg aggacgctgc catgcggaac
     1741 ttgcttcgcc ttgagcttcg cgttgaacgc ggtctcgtcc gcaaacgcga actgcggcgt
     1801 ttcagcgggc agcacggtcg ccatgtaacg gcccggataa atgacgtcgg tcgatacatc
     1861 gtcgccgact ttgaacacaa tgttaggcat aggggaaaag gttaaatgag ttaaatcagt
     1921 tacaattgtt acatgactga catgagcgga atgggtgaag cgtgatgcgt gatgcgtgat
     1981 gcgtgaccgg gtcacttcct cctcggatcg gtgattacgc cggtgatggc cgaggcggcg
     2041 gccacggctg gggaacagag atagacttcg gagttcgggt tgcccatgcg gcccttgaag
     2101 ttccggttgg tggtggacag cgccacttcg ccatcgccga gtgcgccttc atggatgccc
     2161 aggcacgggc cgcagccgga gttcattacc acggcgccgg cttgcatgaa gacgccgaca
     2221 tagcctttac ccagcgcctg ccggtaaatc ttggccgagg ccgggaagat caacatgcgc
     2281 gtgccttgcg cgaccttctg gcccttcatg atcttggcgg caatcgccag gtcgtcgagc
     2341 cggccgttgg tgcaggagcc gatgacaatc tggttcacct tcttgccggc tacctggtca
     2401 attggcttca cgttatccac cgtatgcggg caggcgattt gcggctgcag cttggaaacc
     2461 ttgatctcaa tgacgctctc gtaggcggcg tccgcgtccg gcaacactgg ctcgatcggg
     2521 tccgtcacgc cggcctcttc gcgcaggtag cgcagcgtct cctcgtccgc cgggacgatg
     2581 cctgccgtgg cgccggcttc gaccgccata ttgcacaaca ctagccggcc cgaagtggac
     2641 atgttgcgga tggtctcgcc gtggaactca aggaccttga aattcgcccc ttgcgcgctg
     2701 atcttgccga ccaggtgcag aatcaggtcc tttggcccga caaacttctt gaagcggccg
     2761 gtgacgacca ccttgatggt cgccggcact tccacattca agacgctgcc cagggcccag
     2821 acgctggcca tctcggtcgc gccgatgccg aaccccaatg cgcccaacgc gccatggctg
     2881 gtcgtgtggg agtccgtccc caccaccacg tagcccgggc gcacgtagcc attctccggc
     2941 aggatctggt ggcagatgcc gcccacatcg ccccggatgt cgtgaaacct cccaatgccc
     3001 tgggcaccga tgaactcgcg caccttcttc tgattggagg cagtcttcgg cgactccgct
     3061 gggacgcgat gatcgaagat caccgccacc cggctcgggt cccagatgcg ggacggcagc
     3121 ggcgtgcctt tatagatctc caggaattga ttgatcacca gggccgcatt ctcatgcgac
     3181 atggccagat ccactttcgg ctccacgacg tccccggcct tcacggacgg gaggccggcg
     3241 gcgcgggcaa gaatcttctc agcaacggtc atgctcattg tatgttagtt gatggttgat
     3301 agttaagagt tgatagtcat tcctgtcaga tgacgtgggc ctctttcagg cgcttgatgt
     3361 catccgcaga atagcccaag ccgcccagaa tttcattcgt gtgagcggaa agacgcggcg
     3421 gcggggtctc gatgccaccc ggggtcttct caagcttcgc gctcaggccg aagagctgca
     3481 actcgccgac tccctcaacg gagaccggtt gcaacgtctg acgatgccgc acctgcggct
     3541 ggttcaaggc ggcctccagc ccgaggatcg cgccggaggg cacgtcgttc ctattcagca
     3601 gttcgatcca gtaagcggtc tccttttcct tcagtttcac ttcgagcagg ggcgtcagcg
     3661 ccttgcggtt ctttttccgg ctgtcccgat cctggaaccg agggtcggtc ttcaactccg
     3721 gcatgccgag caggtcggcg acggcttccc actgctcctg cttgttggcc gcgatattga
     3781 tatagccgtc tttagtgaca aaggtgccag atggggcggc ggtgaaattg tcattgccca
     3841 tgagcaccgg ctgctgcttg ccgataagca ggttggccgc gacccagccg gccagcggca
     3901 tgatggaatc cagcagcgcc acatcaatga actgcccctc gccggtccgc tcgcgatgaa
     3961 acaacgcggc gagcacggcg aacgcggcat tgagtccgcc caccgtgtcg cacaccggga
     4021 agccggcacg caacgggtta agccgctcat cgccgttaat cgccatcacc ccgctcaggc
     4081 cctggataat ctggtcgtag gccggcttga aggcatcggg ccccgtctgg ccgaagccgg
     4141 agatggcgca gtaaatcagg cgcggattga tctgggccag caccgggtag tccaggccga
     4201 gccgcttcat gacatcgggc cggaagttct ccaccagcac gtcagccgtc gcgaccagct
     4261 tcttgaagat ctctttgcct tcggggctct tcaggtccag ggtgagcgac ttcttgttgc
     4321 tattctgagc caggaagctg gtgcccatca gttgctggtt gagcttgggc acgttgccga
     4381 gcttgcgcgc caggtcgccg tccttcggat tctcgatctt gatgacttcg ccgcccagca
     4441 gggccaggtg cagggtggcg aacggtcccg ccaggacatt ggtcatatcc agcacccgca
     4501 cgccttgcaa cgccttgcgg ggcttttgtt ctttctcttg ggaattactc ataatcagga
     4561 gcaggccggg gtggccgcag ccagcccgat cttatacacc atgcccggca tttcgcggtt
     4621 gaagaaccgg ctcatatcgc gcgcaatttc gaccagtttc tcctggttga catcggcgcg
     4681 ctggcccagg cgatgcaggt aatacaccag gtcctcggtg cagacgttgc cgccggccac
     4741 cttggtaaag gggcagcccc ccaacccggc cacagaagac tcgaaacacc tcacgcccac
     4801 atccatcgcg gcgcagcagt tggccatgcc caggccgtag gtgttgtgaa aatggcaggt
     4861 gcactcggcg tcaggagcga gcttaagaat ccccccgaac aactcctcga cctgccgcgg
     4921 agtcgcatgt cccgccgtgt cggccaaact gatactgcgg aacccggcat tcaggaactg
     4981 ctccgcgatc ttcagcacgc gctccggggc caccatgcct tcaaagccgc agccaaacgc
     5041 ggactggacc gacaattgca cgcgtttccc cgcgctggtc gccaggcggg ccatcgcgat
     5101 aatgcgcttg gtggcgtcgg cga
//
LOCUS       DATCGS010000223         4687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_317805, whole genome shotgun sequence.
ACCESSION   DATCGS010000223 DATCGS010000000
VERSION     DATCGS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317805"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1252
                     /locus_tag="VIL39_04520"
     CDS             476..1252
                     /locus_tag="VIL39_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5480721.1"
                     /translation="MTEKLEIRVHGDASLPTLVYLPGLHGDWTLVGSFRHAVSDRVRF
                     VELTYPRTLTWSLEDYAREVETALAEQGIVRGWLIGESFGSQLVWPLVARKRFQVEAV
                     ILAGGFVQHPMRWGVRLAERLCGGIPLALLTHILFGYAKVARLRYRNAPETLAHIHEF
                     IARRTELDREAAVHRLRLIAQNDPRPIARQVKLPVYALTGLVDPVVPWCFVRPWLKKN
                     CPSLREYKIIGQADHNVLGTGSRPAAEQVLRWMGEKPFAR"
     gene            complement(1344..1877)
                     /locus_tag="VIL39_04525"
     CDS             complement(1344..1877)
                     /locus_tag="VIL39_04525"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY5480722.1"
                     /translation="MSIVTKGGDQGTTALMYNRRVPKCHPRVEAYGAVDELNAAIGLA
                     RAAAQDGVLRDNLATTQKDLVRLMGELATVVEDLPRYVKDGHSLVTSAMTASLEKWIA
                     QLETAKLSTMGWAMPGANTTSAAFDFARTVCRRAERRACALHDSGQLHNAEIVVYLNR
                     LSDLLWLLARWAEAKQP"
     gene            complement(1943..2920)
                     /locus_tag="VIL39_04530"
     CDS             complement(1943..2920)
                     /locus_tag="VIL39_04530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5480723.1"
                     /translation="MKSAITFCLLVVLVLSGTRRTAEAAQVSGLPYVWLADWAKANGF
                     EVRWLKPDETLQLSKNGSKLVLTVDSRETQINGVQVWLLLPVVARNGKVCLAQLDVER
                     TLQPLLWPTRAQASARIRKICLDPGHGGKDPGNQVGSNQEKQYTLLLAQEVRTQLAQA
                     GFKVTLTRSTDTFIDLPTRSELAKRQSADLLVSLHLNAAETGRDSVQGAEVYCLTPAG
                     ASSTNARKEGSFAGWFPGNRHNDLNLFLAYQVQKALTRNLAVPDRGVRRARFAVLRNA
                     VMPAVLVEAGFMSHPKEGRKIFTAAYRQKMARAIVEGLLAYKRAVERGV"
     gene            complement(3012..3734)
                     /locus_tag="VIL39_04535"
     CDS             complement(3012..3734)
                     /locus_tag="VIL39_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5480724.1"
                     /translation="MKKPTADVHPHTSLPFVFINMAMTADGKIATANRLVSSFGSPCD
                     QDHLLRLRATADAVMTGARTADLNPINMGPGPARYRRRRLRRGLAEFNLRIIVSRSGT
                     VNPKAEVFKHKFSPIIILTTRRATTARLKKLRALVAEVKICGTNEIHFARALRWLRAK
                     WGIKRLVCEGGGELNDALFRAGVVNQLHLTVCPRIFGGGAAPTIADGVGAGSLARAAR
                     LELDSARGRGNEMFLAYRVVKK"
     gene            complement(3731..>4687)
                     /gene="queA"
                     /locus_tag="VIL39_04540"
     CDS             complement(3731..>4687)
                     /gene="queA"
                     /locus_tag="VIL39_04540"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY5480725.1"
                     /translation="DLLEYLHPGDLLVLNDSRVIPARLRGVNAHTGGQFEILLLEENA
                     TNDWWVMLRPGKRARVGTQIALRDTSGQPSPVHATVLEANHEGHRRLSFAGAPNIADL
                     LDVLGEVPLPPYIVRSDKSRMEEDRQRYQTVFAESAGSVAAPTAGLHFTESLLAEISA
                     RSVQACFLTLHVGLGTFAPVKAETLATHTMHEERYELSQQTARDVNEAKAAGRRVIAA
                     GTTTVRVLETLAARRADPSSATVVAGTGRTRIFIHPPYDFKVTDALLTNFHLPRSTLL
                     MLVSAFAAPNDTRGRDVILSAYAEAIRDRYRFFSYGDAMLIL"
BASE COUNT          842 a         1465 c         1448 g          932 t
ORIGIN      
        1 attgctggat gagcccctgg agccgcttgc cggacggatg ggagagaact tccagcgcct
       61 gtttggggtg atagcgccgg cgtgagtttg gaaggggggg cagtggccgc gctgcgtcca
      121 tcgcgtctct gcggcttaac ggaggagagc caccccaaag ccacatcaat gcgatatcaa
      181 agccacccca aagcgtgttg atagccaagc gataggcacc ccaaagccac cttaaagcca
      241 ccttaaagcc accctaaagc caccctaaag ccaccctaaa gcccggaaac cgggctcggg
      301 cactatcagc ttggggcctt gagccttgag ccttcgcttt cggctctcgc ccttgctttg
      361 attgggctgc tgatcgtcat tgtgattggt ctcacgagag ttgccgtctc tttccctaca
      421 ctttccacga acgaaaagac tggctgacct gtcccaagca tcgcatatct cgacgatgac
      481 ggagaaactg gaaatacgcg tccacggcga cgcctcgctg cccacgctgg tttacctgcc
      541 gggcctgcac ggcgattgga cgctcgtggg cagtttccgc cacgcggtca gcgaccgggt
      601 gcgctttgtg gaactgacct atccgcgcac gctcacttgg tcgcttgagg attacgccag
      661 ggaagttgaa acggcactgg cagagcaggg gatcgttcgg ggctggctga ttggggagtc
      721 gttcggttcc cagcttgtgt ggccgctcgt cgcccggaag cggttccagg tggaggcagt
      781 gatcctcgcc ggtggtttcg tccagcaccc gatgcggtgg ggggtgcgtc tggccgagcg
      841 cctctgtgga ggtattccgc ttgccctgct gacgcacatt ctgttcggct atgccaaggt
      901 ggctcgcttg cgctatcgga acgcgccgga gacgctggcc cacatccatg agtttattgc
      961 gcgccgcacc gaactggatc gcgaggcagc cgtccaccgc ctgcggttaa tcgcgcaaaa
     1021 tgatccccgc cccatcgctc ggcaggtgaa gctgcccgtc tatgctctca ccgggctggt
     1081 cgatccggtc gtgccctggt gctttgtgcg gccctggctg aagaagaact gtcccagcct
     1141 ccgcgagtat aagatcattg ggcaggccga ccacaacgtg cttggcaccg gttcccgacc
     1201 tgccgccgaa caagtcttgc gctggatggg cgagaaaccg tttgcccgat agtcgcgcag
     1261 ggcgtccgat tgtttctccg acggctccca agctgcggcg tcagttgccg ctaccaaaac
     1321 cggcggaggt tttctcaagc gcgtcagggc tgcttcgctt cggcccaccg cgccagcagc
     1381 cacagcaaat cggacagccg gttgagatag actacgatct cagcattgtg gagttggccg
     1441 gagtcgtgca acgcgcaggc gcgcctctcg gcgcgccggc acacggtccg cgcgaagtcg
     1501 aacgccgctg aagtcgtgtt cgcccccggc atcgcccagc ccatcgtgga gagcttcgcc
     1561 gtctccaatt gcgcaatcca cttttccaac gaggccgtca tggccgaggt caccagcgaa
     1621 tggccgtcct tgacgtagcg cggcagatct tccaccaccg tggcgagttc tcccatcagc
     1681 cgcaccaggt ccttctgggt cgttgccagg ttgtcccgca gcacaccgtc ctgagccgca
     1741 gcgcgggcca gtccgatagc tgcattcagc tcgtccaccg ccccgtaagc ttccacgcgc
     1801 ggatggcact tgggaacccg tcggttgtac atcaacgccg tcgtgccctg gtccccgcct
     1861 ttggtgacga tgctcataaa gctttaagag cgtggacgcc gggcagtgtg acaacacaaa
     1921 ctcatttctc ggttttcgga tttcacacgc ctcgctcgac cgcccgtttg tatgccagca
     1981 agccttcgac gatggcccgt gccatctttt gccgatacgc cgcggtaaag atcttccgtc
     2041 cttccttggg atgcgacatg aaaccggcct cgaccagcac cgccggcatg accgcgttgc
     2101 gcagcacggc aaagcgcgcc cggcgcaccc cgcggtccgg gacggcaaga tttcgcgtca
     2161 gcgccttctg cacctggtag gccaggaaca ggttcaagtc gttgtgccgg ttgccgggga
     2221 accaacctgc gaacgagcct tccttgcggg cattggtgga actcgcgccc gctggcgtca
     2281 gacaatatac ttccgcgccc tgcacggaat cgcgcccggt ttcggcggca ttgaggtgca
     2341 gactgacaag caaatccgcg ctctggcgct tcgcgagttc cgagcgtgtt ggcaggtcga
     2401 taaacgtatc cgtgctgcgc gtcagcgtca ctttaaaacc ggcctgcgct agttgtgtgc
     2461 ggacttcctg cgccagcagc agcgtgtatt gcttctcctg gttcgagccg acctggttgc
     2521 cggggtcttt gcccccgtgg cccggatcga ggcagatctt tcttatcctt gcgcttgcct
     2581 gggctctcgt cggccacagc agcggctgca gcgtcctttc cacatcaagc tgtgccaggc
     2641 agacctttcc attgcgcgcc actaccggga gcagcagcca gacttggacc ccattgattt
     2701 gggtctcacg cgaatccacc gtcagcacaa gcttcgagcc gttcttgctt agttggagcg
     2761 tctcatccgg tttgagccag cggacctcaa aaccattggc tttggcccaa tcggcgagcc
     2821 acacgtaggg cagtccggaa acctgcgccg cctctgccgt gcgcctggtc cccgaaagga
     2881 ccagcaccac cagcaggcag aacgtgattg ccgatttcat gcccgcgaac tctgccggtc
     2941 gagccttcct tgttcaagca aagagcgatg cggcatgatc aggtgttggc gccgtgtgtg
     3001 aggatcagtc gtcacttttt cacgacccga tacgcgagga acatttcatt gccccgccct
     3061 cgcgctgaat cgagttccag tcgtgccgcc cgcgccaggc tgcctgcgcc cacgccgtca
     3121 gcgatcgtcg gggccgcccc gccaccgaag attcggggac acaccgtcag gtggagttgg
     3181 ttcaccacgc cggcacggaa caaggcgtcg ttaagttcgc cgccgccttc gcagacgagt
     3241 cgtttgatcc cccacttcgc gcgcagccac cgtagcgcgc gggcgaagtg gatctcgttc
     3301 gtgccgcaaa tcttcacttc cgcaacgagt gcgcgcagct tcttcaaccg ggctgtggtg
     3361 gcgcggcgag tggtgagaat gatgatcggg gagaacttgt gcttgaagac ctcggccttt
     3421 ggattcacgg tgccagagcg gctcacgatg atgcggaggt tgaattcggc cagaccgcgc
     3481 ctcaagcgcc ggcggcggta tctcgccgga cccggcccca tgttgatagg gttcaagtct
     3541 gccgtgcgcg cgccggtcat cacggcatcg gcagtggcgc gcagtcggag caggtgatcc
     3601 tggtcgcacg ggctgccgaa tgaggagacg agacgattgg cagtggcgat cttgccatcg
     3661 gcggtcatcg ccatgtttat gaagacgaac ggcagggacg tgtgcggatg aacgtcggca
     3721 gtcggtttct tcatagaatg agcatcgcat cgccatagct gaagaagcga tagcgatcac
     3781 gaatcgcttc ggcgtaagcg gatagaatca cgtcgcgccc acgcgtgtcg ttcggggcgg
     3841 cgaaggcgct gacgagcatc aacagcgtgg agcggggcag gtgaaaatta gtcaggagcg
     3901 cgtcggtgac tttgaagtcg taagggggat gaatgaaaat gcgggtcctg ccggtgccgg
     3961 cgactacagt ggcgctggac gggtcagccc ttcgggcggc gagggtttcc agcacgcgca
     4021 cggtagtagt gccggcggcg atgacgcggc ggcctgcggc tttagcttcg ttaacatcac
     4081 gggcggtctg ctggctcagc tcgtaacgct cttcgtgcat ggtgtgagtg gccagcgtct
     4141 cggctttgac gggcgcgaag gttcccagcc cgacatgcag ggtgagaaag caggcctgca
     4201 cgctacgtgc ggagatttcg gctagcagcg attccgtgaa atgcaatccc gcggtcggag
     4261 ccgcgaccga gccggctgac tctgcgaaaa cggtctggta tcgttgccgg tcctcttcca
     4321 tccggctctt gtccgaacga acaatgtacg gaggcaacgg aacttcgccc aggacatcga
     4381 gcaagtcagc gatgtttggc gcgcccgcaa agcttaggcg ccgatgtcct tcgtgatttg
     4441 cttcgagcac ggtcgcgtgc accgggctcg gctggccact cgtatcccgg agggcaattt
     4501 gcgtccccac tcgcgcccgc ttgcccgggc gcagcatgac ccaccaatcg ttggtggcat
     4561 tctcctcaag caaaagaatt tcaaactgtc cgcccgtgtg agcatttacg cctcgcaggc
     4621 gagcagggat cacgcgcgaa tcgttgagca ccaacagatc gccggggtgc aggtactcca
     4681 gcaagtc
//
LOCUS       DATCGS010000224         4066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_565272, whole genome shotgun sequence.
ACCESSION   DATCGS010000224 DATCGS010000000
VERSION     DATCGS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565272"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VIL39_04545"
     CDS             complement(<1..414)
                     /locus_tag="VIL39_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480726.1"
                     /translation="MASVHQRPRSPYWHASYLAPDGRWILRSTKVVDRQAALAVAMEF
                     ERASKIARRGELVEAQAREVLKDIMKRADMGETLQAVTIKGHFDNWLASKRDRKSAGT
                     WKRYGGAVAEFLKTLGNRASRNLTALAARDVQHFLD"
     gene            complement(518..3103)
                     /locus_tag="VIL39_04550"
     CDS             complement(518..3103)
                     /locus_tag="VIL39_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY5480727.1"
                     /translation="MNWLLLKNSLLVGGLTTLLAASFGFLAALWLAGLEGRWRNRLFA
                     VAVMALALPPFVVTNCWLHFLGYAGVWRGWLPLNIVSLGGTVWILSLLTWPITLLMVL
                     SAWQRLEPPQLESDVAVRGWWLIRGLLWPLGRTALAQAAVLTFVLAMNNFAVPAILQV
                     KVFPAEMWVLFNTTFDTVGALQLSWPLVVAPLLLLLWFARREFPWPRTEGPVSAKLFR
                     QQLGPGWFWACGTCTLLLCLLAVGLPVFQLVSVTRTWTELPGALAAGQGAIWNSLSLA
                     AASATAVIALGLAAGAHRTLRNSAFGFPSVFGFRISDFCLDLCRWLAWLPFLLPGVLL
                     GIGLIVLLNRPWLAFLYQSAAIVILAFVIRYLALGWNAAARAFQTVDRDLTDVARLDG
                     ASRWQMLRHVQWPQIAPQAAAAWYVVFLLCLWDVESMILVVPPGGETLALRVFNLLHY
                     GHNAQVNALCLTLLVLAVTPLAIWKAWCVVRGALSVHRMDVTPPTPSLPTKPGNIQHP
                     TSNTQHPMVAEAEAAGCSMLDVGCWMFPFRPDGLTLLFASAVMAILTGCAPNTPSDQA
                     PLQSQLFQRVQIIGSRGVGVGQLNKPRSVAVDARDNLYVADMTGRVQKFSSNGVFLLS
                     WQMPLTTLGKPKGMSRDREGNIIVLEPHYQRVNVFSPEGKLVAQWGKRGTNAGELMMP
                     RAVAVNSRHEVFLSEYGLVERVQRFASKSAAVLSRSPSPQLLASFGHSGNGPGDFNRP
                     EGLCVDSQDRLYVADSCNHRIQVFSSDGKFLRAYGKAGKGKGELSYPYDICVDAAGRQ
                     YVCEFGNSRIQVFDANDRPIEIIGGPGLAPGQFNNPWGVALDSAGNLYVADSQNHRVQ
                     KLIRK"
     gene            complement(3108..>4066)
                     /locus_tag="VIL39_04555"
     CDS             complement(3108..>4066)
                     /locus_tag="VIL39_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480728.1"
                     /translation="RLTVRDDMAVDNQASVVSLLPKPVKVLLISRGNRLLEKALRAVA
                     NVELAGATDLTDTATVFDFVVLDDVTPTVWPKGNVLAIHVVNTNWLEGVRREEAPAIV
                     DWRSAHPLLRYTGFDNVQVVQSLTARTPTWAVSLVETTQAPIILAGELGRQRIIWIGF
                     DILESNWPLRVSFPIFIANAVDWLNPANVRSGQLLVKAGDPFRLPLSEPASSAQVTLP
                     DGTTKSLKLDPTANEFVFGDTSKSGVYRVRLGTNDTVFCANLLDAAESSIKPRDELQL
                     GKYTKVIATTTKRANMEVWRTIAALGLLVLLAEWWYYHRRTV"
BASE COUNT          820 a         1280 c         1257 g          709 t
ORIGIN      
        1 gtctaggaag tgttgcacgt ccctcgctgc gagggctgta aggttcctgc tggcgcggtt
       61 gcccagcgtc ttcaaaaact ctgccacggc cccgccgtag cgtttccagg tgcctgcaga
      121 cttccgatcc cgcttgctgg cgagccaatt gtcgaaatgc cctttgatgg tcacggcttg
      181 caaggtctcg cccatgtccg cccgcttcat gatgtccttc aggacttcgc gggcctgggc
      241 ttccaccagt tccccgcgcc gggcgatctt ggaagcccgc tcaaattcca tcgccacggc
      301 cagggcggct tggcggtcaa ctaccttggt gctgcggaga atccaccggc catctggcgc
      361 gaggtatgag gcgtgccagt atggtgaacg aggtctttga tgtacgcttg ccatatcagt
      421 gctaaagcct agtgctaaag cctgcctatg tcaaccatgt tcaaacatgc tgataataaa
      481 gggttttatg cgtgtccacc ttgtgggaat tggaagttca tttccggatg agcttctgca
      541 cgcggtggtt ctgcgagtcg gcgacgtaaa gattgcccgc cgagtcgagc gccacacccc
      601 agggattgtt aaactgtccc ggcgccaagc ccgggccgcc gatgatttca atgggccggt
      661 cgttcgcatc aaatacctgg atgcggctat tgccgaattc gcagacgtat tgccgccccg
      721 ccgcatccac gcagatgtcg taggggtagc tcagctcgcc cttgcccttg cccgccttgc
      781 cgtaggcgcg gaggaacttg ccgtcactgg agaagacctg gatgcggtgg ttgcacgagt
      841 cggccacgta aagccggtct tgcgaatcaa cgcagagtcc ttccgggcgg ttgaagtcgc
      901 cggggccgtt gccggaatgg ccaaagctgg ccagcaattg aggggagggt gagcggctga
      961 ggaccgccgc gcttttggaa gcaaagcgct gcacgcgctc gaccaggccg tattcactca
     1021 ggaagacctc atggcgggaa ttcaccgcta ccgcgcgagg catcatcaac tcacccgcgt
     1081 tggtgccccg cttgccccac tgcgcgacca gtttgccctc gggcgaaaac acattcacac
     1141 gctgatagtg cggctccagg acgatgatgt tgccctcgcg gtcgcggctc atgcccttgg
     1201 gcttgccgag ggtggttaac ggcatttgcc aggaaagcag gaagactccg ttcgaggaga
     1261 acttctgcac gcggcccgtc atgtccgcca cgtagaggtt atcccgcgcg tccaccgcca
     1321 ccgagcgtgg cttgttgagc tggcccactc cgactccacg cgagccgatg atctgcacgc
     1381 gctgaaacag ttggctttgc aggggagcct ggtctgacgg cgtgttgggc gcacagccgg
     1441 tgaggatggc cataacagcg gaggcgaaca ggagcgtcag accgtccggc ctgaagggaa
     1501 acatccaaca tccaacatcc aacatcgaac aaccagcagc ctccgcttcg gcaaccattg
     1561 gatgttgggt gttcgatgtt ggatgttgga tgttcccggg tttcgtgggg agggacgggg
     1621 tgggtggagt tacatccatt ctgtggaccg acagcgcacc acgcaccacg caccacgctt
     1681 tccaaatggc cagcggggta accgccagga ccagcagggt gaggcaaagc gcgttcactt
     1741 gcgcgttgtg gccgtaatgc agcaggttga acacacgcag cgccagggtc tctcccccgg
     1801 gcggcacgac gagaatcatt gactccacgt cccagaggca aagcaggaac accacatacc
     1861 acgcggcggc ggcttgcggt gcgatttgcg gccactgcac atggcgcagc atttgccagc
     1921 gcgatgcccc gtccaatcgc gcgacgtcag tcaaatcgcg gtccaccgtc tggaaggcgc
     1981 gagccgcggc gttccagccc aacgccaggt aacgaatcac aaacgccaga atgacaatcg
     2041 ctgcgctctg ataaaggaac gcgagccagg gccgattcag taggacgatc agcccgatgc
     2101 ctagcaatac ccccggcaag aggaaaggca gccaggcgag ccagcggcag agatccaggc
     2161 agaaatccga aatccgaaac ccgaataccg aaggaaatcc gaaggccgaa ttccgaaggg
     2221 ttcggtgcgc tccggcggcc agacccagcg cgatgaccgc tgtggccgaa gcggcagcga
     2281 gcgagagcga gttccaaatg gcgccttggc ccgcagccag cgccccaggc aactcggtcc
     2341 aggtccgagt gacggaaacc aattggaaga caggcagacc gacggcgagg aggcagagca
     2401 ggagggtgca ggtgccgcaa gcccagaacc aaccgggacc gagctgctgg cggaagagtt
     2461 tggctgatac cgggccttcg gttcgcggcc atgggaattc gcgccgggcg aaccacagca
     2521 gcaggagcag cggcgccacc accagcggcc aactcaactg gagcgcgccg acagtgtcga
     2581 aggtggtgtt gaagagcacc cacatttccg ccgggaagac tttcacctgc aagatggccg
     2641 gcacggcaaa gttgttcatg gcgagcacaa aggtcagcac cgctgcctgg gccaacgcgg
     2701 ttcggcccag gggccagagc aggccgcgaa tcagccacca gccccggacc gcgacgtcgc
     2761 tttccaattg cggtggttcg agccgttgcc aggcgcttag gaccatgagc aacgtgatcg
     2821 gccaggtcag caggctgaga atccacaccg ttccgccgag tgaaacgatg ttcagtggca
     2881 accagccgcg ccacacgccc gcgtagccga gaaagtgcag ccagcagttg gtcaccacga
     2941 atggtgggag ggcgagggcc atcacggcca ccgcgaacag gcgattccgc cagcggccct
     3001 ccagtcccgc cagccacagc gccgccagga agccaaagct cgccgccagc agcgttgtga
     3061 ggccgcccac cagcaaactg ttcttgagca ggagccagtt cacctcttca cacggtcctc
     3121 cgatggtagt accaccactc cgccagcaac accaggagcc cgagcgccgc aatggtgcgc
     3181 cacacttcca tgttcgcccg cttcgtcgtg gtcgcgatta ctttggtgta tttgcccaac
     3241 tgcaattcgt cgcgcggctt gatgctgctc tccgccgcgt cgagcaggtt ggcgcagaaa
     3301 accgtgtcgt tcgtccccag gcggacccgg taaacgcccg acttggaggt gtcgccgaac
     3361 acgaattcgt tggcggtcgg gtcgagcttg agcgatttcg tcgtgccgtc gggcagggtg
     3421 acttgggcac tggacgccgg ttccgatagc ggcaggcgga aggggtcgcc ggctttcacc
     3481 agcagttgac cgcttctcac gttggccggg ttcaaccagt ccaccgcgtt ggcgataaag
     3541 atggggaacg agacgcgcag cggccagttg ctttccagga tgtcgaagcc gatccaaatg
     3601 atgcgttgcc ggcccagctc gcccgccagg atgatcggcg cttgcgtcgt ttccaccaac
     3661 gacaccgccc aggtaggcgt tcgcgccgtg aggctctgca ccacctgcac gttgtcaaag
     3721 ccggtgtagc gcaggagcgg atgtgcagac ctccagtcca caatcgccgg cgcctcctcg
     3781 cgtcgcaccc cttcgagcca gttcgtattg accacatgaa tcgcgagcac gttgcccttt
     3841 ggccagaccg tcggcgtcac gtcatccagc acgacgaaat caaacaccgt ggctgtgtcc
     3901 gtcaaatccg tggcgccggc cagctcgaca ttcgcgaccg cccgcaaggc cttctccaat
     3961 agccggtttc cccgtgagat caggagcacc ttcaccggct tcggcaatag gctcacgaca
     4021 gaggcctggt tatccacggc catatcatcc cggacggtca ggcgag
//
LOCUS       DATCGS010000225         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_267839, whole genome shotgun sequence.
ACCESSION   DATCGS010000225 DATCGS010000000
VERSION     DATCGS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267839"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..2048)
                     /gene="ftsH"
                     /locus_tag="VIL39_04560"
     CDS             complement(30..2048)
                     /gene="ftsH"
                     /locus_tag="VIL39_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5480729.1"
                     /translation="MSDDNKQRDDDKNSKKKGGEFRVPPRTMVVWIAILGGITLLMLF
                     KDRMDTPGDVLKQYGFQQLVESNRIIQATINYNPQSPDLREITGRYWKDNDHKVEAAF
                     RAKVVLYPELQKKLLSMPQFEPREPNTMLFSFLLSVLPIIVIAALIWFFFIRQIKMAG
                     KGALSFGKSKARLLAKERNKTTFKDVAGVEEAIQEVSELVEFLKDPKKFQRLGGRIPK
                     GVLMIGPPGTGKTLLAKAIAGEADAAFFSISGSDFVEMFVGVGASRVRDMFEQARKAT
                     PCLVFIDEIDAVGRSRGHGLGGGNDEREQTLNALLVEMDGFDTQEGIIIIAATNRPDV
                     LDPALLRPGRFDRQVTVNLPDVRGREGILKVHAKNVKLAPAVDLSVIARGTPGYSGAE
                     LANLLNEAALLAASTNKKAVGMEELEEARDKVRWGRERRSLAMTDEDKKCTAWHEAGH
                     ALVNVMLQHTHPLHKVTIIPRGQSLGSTMSLPKTDVLNRRRKEMLDMIAVMMAGRISE
                     EILSGDISSGAAGDIQQATNMARAMVTQWGMSDRLGMIQYGDDDEFVFLGREVSRAKV
                     YSEFTSQEIDSEVKRIIDNAYKIAKDLLTTNIEKLELIAKSLLEYETLDGEQVMEIVS
                     TGKFTAPPPPPQVEPPLGAPAGTPLPETPSKPTPPKLPGLGTPAPATA"
     gene            complement(2106..3608)
                     /gene="tilS"
                     /locus_tag="VIL39_04565"
     CDS             complement(2106..3608)
                     /gene="tilS"
                     /locus_tag="VIL39_04565"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY5480730.1"
                     /translation="MSELPDHVEQCIRTRRLFRPGERILVAVSGGADSMVLLHVLHGL
                     AAKHRWQLTVAHLNHQLRGRSSAADERLVRRTAERLKLPVLVEGADVRQSAHTLKLSL
                     EMAARKLRHHFLAATAAQLRIPSIALAHHADDQLELFFLRLLRGSGGEGLAGMKWRNP
                     SPSDPEIELVRPLLDQPKAVLIEYAADRGIQYREDASNAVLDFQRNRIRHELLPLLRR
                     KYQPALDRTIPRVMDIVGAEAEFAAAAAREWLDCMGSPKSKVQSPKCGEVRGTFSRLA
                     RGQVGRRAGLAFEELPVAVQRRCVQFQLLSQGIVADFELVEQLRLAADRPVPVSRAQA
                     QRDARRTSGRPVPLCAVRDRLGLVHLQAAEAEEFKTDSAAVYLEDRAGEVEFDGARIW
                     WRIHSRSAVGQPKARVECECFDADKVGSPVWLRHWRPGDRFQPIGMPGPVKLQDFFTN
                     QKVSRGRRRQLIVAATAKGEVFWVEGMRISERFKLSNQTIRRLQWRWERL"
     gene            3859..>4113
                     /locus_tag="VIL39_04570"
     CDS             3859..>4113
                     /locus_tag="VIL39_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5480731.1"
                     /translation="MSVAAEIKATPTLQTLPGDDLRQIMWRFADRYDLAMLVQSTRTV
                     ARGPVARLVAQGGRNSHEWTPEKARLLTAFDESGITAAFLD"
BASE COUNT          780 a         1399 c         1088 g          846 t
ORIGIN      
        1 tgtaactctt gtaactcatg tgacctatct caggcggtcg ctggtgcagg agtccccagg
       61 cccggcagct ttggcggagt cggtttgctc ggggtttccg gcaacggtgt ccccgccgga
      121 gcgccaaggg gcggctcaac ctgtggcggt ggtggcggtg cggtaaactt gcctgtgctc
      181 acaatctcca tgacctgttc cccgtcgagc gtctcgtatt caagcagcga cttggcaatc
      241 agctccagct tctcgatatt ggtggtaagg aggtctttcg cgatcttgta agcattgtca
      301 atgatgcgct tgacctcgct gtcaatttcc tgggaggtga attcgctata gaccttggcg
      361 cgcgacacct cgcgcccaag gaagacgaat tcgtcatcat ccccgtattg tatcatgccc
      421 agccggtcgc tcatgcccca ttgcgtgacc atggcgcggg ccatattcgt cgcctgctga
      481 atgtcccccg ccgcgccgct ggaaatatcc cccgaaagga tttcctccga gatccgaccc
      541 gccatcatga ccgcaatcat atccagcatt tccttgcggc ggcggttgag cacatccgtt
      601 ttcggcagtg acatggttga accgagcgac tggccgcgcg gaatgatcgt aactttgtgc
      661 agtggatgcg tgtgctgcag catcacattg accagtgcat gcccggcttc gtgccaggcc
      721 gtgcacttct tgtcctcgtc cgtcatggcc agactgcggc gctcgcggcc ccagcgcacc
      781 ttgtcgcgcg cctcctcgag ttcttccatg ccaacggcct tcttgttcgt gctggccgcc
      841 agtaacgccg cttcgttcaa caggttcgcc aactcagcgc cggaataacc cggcgtgccc
      901 cgcgcaatga ctgacaggtc aaccgcgggc gccagcttca cattcttggc gtggaccttg
      961 aggatgccct cgcgaccgcg cacgtctggc aagtttaccg tgacctggcg atcgaaacgg
     1021 cccggccgca acagcgccgg atccaggaca tccggccggt tcgtcgccgc gatgataatt
     1081 atcccttcct gcgtgtcgaa accatccatc tcgaccaata gggcgttcag tgtttgttcg
     1141 cgctcgtcgt tgccaccccc caatccatgt ccccggctgc ggccaacggc gtcaatttcg
     1201 tcaatgaaga ccaggcacgg cgtggccttg cgcgcctgtt caaacatgtc ccgcacacgg
     1261 ctggcgccga cgccaacaaa catctccacg aaatccgagc cgctaatact gaagaaggcg
     1321 gcatccgcct caccggcaat cgccttggcc agcagcgtct tgcccgtgcc cggcgggccg
     1381 atcatcagga cgcctttcgg gatgcgcccg cccagccgct gaaacttctt cgggtccttg
     1441 aggaactcca ccaattcgga aacctcttgt atggcttcct cgaccccggc cacgtctttg
     1501 aaggtcgtct tgttccgctc tttggccagc agccgcgcct tgctcttgcc aaagctcagc
     1561 gcgcccttgc ccgccatctt gatctggcga atgaagaaga accagatcag cgccgcaatg
     1621 acgataatgg gaaggacgct cagcagaaaa ctgaacagca tggtgttcgg ctcgcgcggc
     1681 tcgaactgcg gcatgctcag cagcttcttt tgcaactccg gatacagcac gaccttggca
     1741 cggaacgcgg cctcaacctt atggtcgtta tccttccagt acctgcccgt aatttctctc
     1801 agatccgggc tttgcgggtt gtaattgatg gtagcctgaa taatccggtt cgattcgacc
     1861 aactgctgga acccgtattg cttcagaaca tcgcccggcg tgtccatccg atccttgaac
     1921 agcatgagca aggtgatgcc tccaagaatt gcaatccaca ccaccatcgt acgcggcggc
     1981 accctgaact cgcccccctt cttcttggag ttcttatcgt cgtcgcgctg cttattgtcg
     2041 tctgacatct ttgatccggt tctgggaagc ttagcacgct tgcccgaaag ccgcaatacg
     2101 cccatttaaa gcctttccca gcgccattgc aagcggcgaa tcgtctgatt tgacagtttg
     2161 aaccgttccg aaatccgcat cccctccacc caaaacacct ctcctttcgc cgtcgcagcc
     2221 acaataagct ggcgacgccg accccgcgaa accttctgat tcgtgaagaa atcctgcaac
     2281 ttaaccgggc ctggcatgcc aatgggctga aaacggtcgc ccggccgcca gtgccgcagc
     2341 caaaccgggg aaccaacctt gtcggcatcg aaacactcgc attccaccct ggctttgggc
     2401 tgccccaccg cgctccgcga atggattcgc caccaaattc ttgccccgtc gaactcgact
     2461 tcccctgccc tgtcctccaa atagaccgcc gcgctatccg tcttgaactc ctccgcctcg
     2521 gcggcctgca aatgcaccag ccctaaccgg tcgcgaaccg cgcaaagtgg gacaggtctc
     2581 ccgcttgtcc gccgagcatc tcgttgagcc tgtgcccgac tgacgggaac cggccggtcc
     2641 gccgccagcc gaagctgttc caccagctca aaatccgcca cgatgccttg actcagcaac
     2701 tggaactgca cgcagcgccg ttgcaccgcc accggcaact cctcgaatgc gaggcccgcg
     2761 cgccttccga cctgcccccg tgccaaccgg ctaaaggtgc ctctaacctc tccgcacttt
     2821 ggactttgga ctttggactt tgggcttccc atgcaatcca accactccct tgccgctgcg
     2881 gcagcgaact ccgcttccgc cccaacaatg tccatcacgc gtgggatggt tctgtccagg
     2941 gccggctgat acttgcggcg caacaagggc agcaattcgt gcctgatccg gttcctctga
     3001 aagtccagca ccgcattgct cgcgtcttca cgatactgaa tcccacggtc ggcggcatac
     3061 tcgatcaaca cggccttcgg ctgatccagc aacggacgca cgagctcgat ttccgggtcg
     3121 ctgggtgacg ggttccgcca tttcatcccg gccagccctt ccccgccgct gccacgcaac
     3181 agccgcagga agaacagctc aagctggtca tccgcatgat gcgccagcgc gatgctgggg
     3241 attcgcagtt gcgcagccgt ggcagccaga aaatggtgac gcaacttgcg cgcggccatt
     3301 tccagggaga gtttcagagt gtgtgccgac tggcgcacat ctgccccctc aaccagcacc
     3361 ggcagcttca gccgttccgc cgtgcgccgg accagccgct catcggcggc gctgctccgg
     3421 ccacgaagct gatggttcaa atgcgccacg gtaagttgcc aacggtgctt tgctgccagt
     3481 ccatgcaaca catgcaacag gaccatcgaa tccgccccgc ccgataccgc caccaggatg
     3541 cgctcccccg ggcgaaagag ccgccgcgtg cggatacatt gctcaacgtg gtctggcagt
     3601 tcactcacgt ccggatgcta tttcagcagc cggatgaagc aaggaggatt ccgcgccggg
     3661 cggggcggtc ctctcggacc gttgcgctcg gataacacaa tctcgattac tccaacgcat
     3721 ctcctatgag cactttggct tgcgccccgt ctctgaattg cctacaagct tacctgcctc
     3781 gcgtaccgtg ccctgaataa gtccggcgaa aacgcgggat gctgaatact taaatctgtt
     3841 cgatcaacac aatcatatat gtctgtagct gccgagatca aagcgacacc cacgctccaa
     3901 accctgccgg gggacgacct acgtcagatc atgtggcgct tcgccgaccg gtatgacctc
     3961 gccatgctcg tccaatccac ccgcaccgtg gcgcgcgggc cggtcgcccg gttggtcgcc
     4021 cagggtggac gcaattctca cgaatggacg cccgagaagg cccgcctgct gaccgccttc
     4081 gacgagagcg gcatcacggc cgcgttcctg gac
//
LOCUS       DATCGS010000226         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_215726, whole genome shotgun sequence.
ACCESSION   DATCGS010000226 DATCGS010000000
VERSION     DATCGS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215726"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VIL39_04575"
     CDS             complement(<1..875)
                     /locus_tag="VIL39_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5480732.1"
                     /translation="MTRLKVVGSSPNRIDGRDKISGAATYVDDLAFGPDLLHAEVVES
                     PHAHALIKSIDTSAAEKVPGVVRVVTGKDFPYRFGLYMQDRFIFPTDRVRFVGEQVAA
                     VVARDAKIAKRAAKLVKVVYKELKPVLDLTEALRDGAVLVHPDLGKYPRCPWFFPQAG
                     TNIAHFRKTRKGDVEKGFKEADVILEDTYTVPRYAHCAIEPHAIVGLYDLAGRLTLWS
                     ASQSPYTQRHVFAQALAPLGLTHKDIRVVTPYVRGGFGGKAGVSMEIIGAALAIMVKG
                     HPVKVRWSRAQEFYNT"
     gene            complement(872..1345)
                     /locus_tag="VIL39_04580"
     CDS             complement(872..1345)
                     /locus_tag="VIL39_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5480733.1"
                     /translation="MNKRITFKLNQETRAVEVEPSDILLDVLRDKLGLKSPKIGCERG
                     DCGACTVLLNGKSVRTCLILAIEVDGCEITTVEGLSRAGLTPLQESFLKHDSFQCGFC
                     APGIVLSATELLGKNPHPSEAEVKEAIAGNLCRCTGYKPIIEAVLAVSGKSGGRV"
     gene            complement(1355..2245)
                     /locus_tag="VIL39_04585"
     CDS             complement(1355..2245)
                     /locus_tag="VIL39_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY5480734.1"
                     /translation="MAIAHQFEYVKPPTLREAVRILARYGTRARVLAGGTDLVSLISD
                     GVAAPEAVVDIKGIPGLDRIEFKNGVLSIGALVTFNELRESPIIAWKFPVIREMTGWV
                     ASVGIRNRATMVGNLCSAVPCCDSGAVLLVYDAAVQVAGPAGKRKVPLREWFLGPRKT
                     VLKRGEIATGVTVSLPKQKHEACFIKLRRYEGEDLAQASVTVLALAGNHYRISFGAVA
                     PRPIAAERIEVLLEGQALSDDLIQQAVRLVPEEIAPITDIRASKEYRLHMVGVMLERG
                     LRAAVARLAGTGPDYGTSLI"
     gene            complement(2254..2460)
                     /locus_tag="VIL39_04590"
     CDS             complement(2254..2460)
                     /locus_tag="VIL39_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY5480735.1"
                     /translation="MKRLTAILYPGELRRWLVAFNPETGTTIQEQTVGQAVRNLTEAT
                     ELYLEEDLPQRAWASLTPFELAQA"
BASE COUNT          487 a          942 c          829 g          494 t
ORIGIN      
        1 gtgttgtaaa actcctgcgc ccgcgaccag cgcactttca caggatggcc cttgaccatg
       61 atcgccagcg cggcgccgat gatctccatg gacacccccg ccttgccgcc aaagcctccc
      121 ctcacgtagg gcgtgacgac ccgaatgtcc ttgtgcgtca agcccagcgg cgccagcgcc
      181 tgcgcaaaga catgccgctg cgtgtagggc gactgcgaag ccgaccagag cgtcagtcgc
      241 ccggccaaat cgtagaggcc gacaatggca tgcggctcga tcgcgcagtg ggcgtagcgc
      301 gggacggtgt aggtgtcttc caggatgacg tcggcttcct tgaaaccctt ttcgacgtcg
      361 ccctttcggg tcttgcgaaa gtgcgcgatg ttcgtccccg cctgggggaa gaaccacggg
      421 cagcgcggat acttgccaag gtccgggtga accaggaccg cgccgtcccg cagcgcctcg
      481 gtgaggtcga gcaccggctt gagttcctta tagaccacct tcacgagctt cgccgcgcgc
      541 ttggcgatct tcgcatcgcg cgccacgacg gcagcgacct gttccccgac aaaccgcacc
      601 cggtcggtcg ggaagatgaa ccggtcctgc atgtacaacc cgaaccggta agggaaatcc
      661 ttgcccgtaa ccacgcgcac tacgccggga accttctccg ccgcggacgt atcaatactc
      721 ttaatgagcg cgtgcgcgtg cgggctctcg acgacctccg cgtgcagcag atcgggaccg
      781 aacgcgaggt cgtccacgta ggtggcggcc ccgctgatct tatcccgccc atcaatgcgg
      841 ttaggcgaac tgcctacaac cttgagccgg gtcatacgcg cccccccgat ttgccgctca
      901 ccgccagcac cgcttcgatg atgggtttgt agccggtgca ccggcacagg ttgccggcaa
      961 ttgcctcttt gacctccgcc tcggaaggat gcggattctt ccccaacaac tcggtcgccg
     1021 aaagcacgat gcccggcgcg cagaacccgc actgaaagga gtcgtgtttg agaaacgact
     1081 cttgcagcgg cgttagccca gcgcggctca acccctcgac cgtcgtgatc tcgcagccgt
     1141 ccacctcgat ggccaggatg aggcacgtcc gcaccgactt gccgttgagc aggactgtgc
     1201 atgctccgca gtcgcctcgc tcgcagccga tcttcgggct cttcagcccc agtttatccc
     1261 gcagcacgtc caggaggatg tcgctgggct cgacctccac ggcgcgcgtc tcctggttga
     1321 gcttgaaggt gatgcgtttg ttcatggccg cctcctagat caagctggtc ccgtagtccg
     1381 gtcctgtgcc ggccagccgg gcgacggcgg cccgcaagcc gcgttcgagc atcacgccga
     1441 ccatgtgcag ccggtattcc ttgctcgccc gaatatccgt gatgggcgcg atctcctccg
     1501 gcacgagccg cacggcctgc tggatcaaat cgtccgaaag cgcctggcct tccaacagga
     1561 cttcgattcg ttccgcggcg attggacggg gtgcgaccgc gccaaaggaa atgcggtagt
     1621 ggttgccggc cagcgcaagc acggtgacac tggcctgggc gagatcctcg ccctcgtaac
     1681 ggcgcagctt gatgaagcag gcctcgtgtt tctgcttcgg caaggacacg gtgactcccg
     1741 tcgcaatctc gccgcgcttc aagacggtct tgcgcggccc cagaaaccac tcgcgcaacg
     1801 gcaccttgcg cttgccggcg gggccggcca cctggaccgc cgcatcatag accagcagca
     1861 cggccccgct gtcacagcag ggcacggccg agcagagatt gccaaccatc gtggcccggt
     1921 tccgaatgcc caccgacgcc acccagcccg tcatctcccg gatcacagga aacttccagg
     1981 caatgatcgg cgattcccgc agctcgttga acgtgaccag ggcgccgatg gagaggacgc
     2041 cgttcttgaa ctcgatccgg tcaagaccgg ggatgccctt gatgtccacc acagcctccg
     2101 gtgcggccac gccgtcgctg atgaggctga ccaggtccgt accgcccgcg agtacccgcg
     2161 cgcgcgtgcc atagcgcgcc agtatcctga ccgcctcgcg cagtgtggga ggctttacat
     2221 attcaaattg gtgggcgatg gccattatgc tgcttaggcc tgggccaact cgaagggggt
     2281 caaagacgcc cacgcgcgtt gcggcaaatc ctcctctaag taaagctctg tggcctccgt
     2341 gagattccgc accgcctgcc ccaccgtctg ctcctggatt gtggtgcccg tctccgggtt
     2401 gaacgccacc agccaccgcc tcaactcgcc ggggtaaagg atcgccgtca aacgcttcat
     2461 ggcgccattc tagcccaccc cgccgtgccc ggcaatgctc gaaacgacgc cgcaatccga
     2521 cgggcggggt tgcttcaagc tcgcgtttag gcgtgctggt ttgggacgcc tcgccgaggc
     2581 gtccgcaccc gcgcatacac ggacgtttcg gcgaaacgtc cctgccacac ctggtcggaa
     2641 tcgagagctt gatgcagccc tggcgggcgt atcttggcac tgccaccggg acggcgcgcg
     2701 gacattcctg tccgcagcaa tgttcgatgg atagaatggc ttgggaactg gc
//
LOCUS       DATCGS010000227         2812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_365667, whole genome shotgun sequence.
ACCESSION   DATCGS010000227 DATCGS010000000
VERSION     DATCGS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365667"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VIL39_04595"
     CDS             complement(<1..685)
                     /locus_tag="VIL39_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480736.1"
                     /translation="MAERSGDTAFSDRAWFPKRRGASLPAALQILWLQRIFFLVGLAV
                     LLVGCGKKHEPQAAPGESLPPATVRVQKIETIRQPAMEEVVGTVQPKLRAVIEAKNSG
                     RITRLPITLGQSVKQGDMLVELATQEIQARLDQANAAFRQAELDFNRTDNLRKQQATT
                     QAELDAAQARYIVAKATVAEAEALSGYAKIVAPFDGVVARKLADEGDLAMPGKPLLEL
                     EGRTGLRLVA"
     gene            complement(748..2061)
                     /locus_tag="VIL39_04600"
     CDS             complement(748..2061)
                     /locus_tag="VIL39_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5480737.1"
                     /translation="MTKQLGILGLLLSWSLSALCAEPWTLERALEQALASNPDARLAQ
                     QRIVSAQAGLEQANAAFWPRVQFQSSYTGSDNPMLAFGSILSQRAYSSSLNFNDVPSV
                     DDLNARGLVTVPLYAGGKNTATRKAAKANTDAARQDNEAVRNALGFEVSRAFYTVLKT
                     RRFIRAAEAGVNSFEGSLAVAKKRLDGGTLLKADVLDIEVRLAQAREELVRARNANAL
                     AVRVLRNLLGIESGDFEVADTAPSVNAPDSGDFSGRPELAAARHREQSAQEQARAAKG
                     GYLPRVNAFGSMDYDYGWKYENGGSSYTAGALLQWDLWDGKLTRAKVREANANLESAR
                     EEQRKLRLALDLEVEQARLELKAANERLSVTDQVVAQASESVSLTRARFEQELALSSQ
                     LIDAETALVAARVRRAEAEADRQIAIAAARKALALPQLESRSQSK"
     gene            complement(2058..2291)
                     /locus_tag="VIL39_04605"
     CDS             complement(2058..2291)
                     /locus_tag="VIL39_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY5480738.1"
                     /translation="MKMQLIIRRFAGAFILVSLVLAHYHSPYWLWFTAFVGFNLLQSS
                     FTNFCPLETILRKAGMGGSCCGSEKAGDSCCAK"
     gene            complement(2318..2485)
                     /locus_tag="VIL39_04610"
     CDS             complement(2318..2485)
                     /locus_tag="VIL39_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HEY5480739.1"
                     /translation="MILRIVIGVVVGGGLGFAFYKFVGCSTGTCPLTSNPFISTLYGG
                     LVGALVASSIH"
BASE COUNT          503 a          866 c          901 g          542 t
ORIGIN      
        1 cggctaccaa tcgcaagccg gtgcggcctt ccagttcgag cagcggtttt ccgggcatcg
       61 ccagatcgcc ttcgtccgcg agcttgcggg ccaccacgcc gtcaaacggg gcgacaatct
      121 tcgcgtagcc ggacagcgcc tccgcctccg ccacggtggc tttggcgacg atgtagcgcg
      181 cctgtgcggc gtctaattcg gcctgcgtgg tggcctgctg cttgcgcaga ttgtcggtgc
      241 gattgaaatc cagctctgcc tgccggaacg cggcgttggc ctggtcgagc cgggcctgaa
      301 tctcctgggt ggcaagttcc accagcatat ccccctgctt gaccgactgg ccgagcgtga
      361 tgggcagacg agtgatgcgc ccactattct tcgcttcaat caccgcccgc agcttcggtt
      421 gcaccgtgcc gacgacttcc tccatggccg gctgccttat cgtttcaatc ttctgcacgc
      481 ggacggtcgc gggcggcagg ctctcgccgg gtgccgcttg cggttcatgc ttcttgccgc
      541 agccgacgag gaggactgca aggcccacaa ggaagaagat gcgctgcagc cacaggattt
      601 ggagtgcggc gggaagcgaa gcgccacgcc gctttgggaa ccacgctcgg tctgagaaag
      661 cggtgtcgcc gctgcgctct gccaccgcac tccatatggt tgtcgtcctg gcgcgaagtg
      721 gcaagattgt ggtagagacg gggttgctca tttcgattgt gatcgggatt ccagttgcgg
      781 cagggccagc gccttgcgcg cggcggcgat ggcgatctgg cggtcggcct cggcctcagc
      841 acggcgcacc cgggcggcga ccagcgcggt ttcggcatca atcaactgcg aagagagcgc
      901 cagttcctgc tcgaaccggg cgcgggtgag actgacactt tccgatgcct gggcgacgac
      961 ctggtcggtc acgctgagac gttcgttggc ggctttgagt tcgaggcggg cttgctcgac
     1021 ttcaaggtcg agtgccagac gcagcttgcg ctgctcttcg cgggcggact caagattagc
     1081 gttggcctct cggactttgg cgcgggtcaa cttgccgtcc cagaggtccc attggagcag
     1141 ggcgcctgcg gtgtagctgc tgccgccgtt ttcgtatttc catccgtagt cgtagtccat
     1201 gctgccgaag gcattgacgc gggggaggta gcctcctttg gcggcgcgcg cctgctcctg
     1261 agcggactgc tcacgatgcc gggcggcggc cagttccggg cgcccggaga aatcacccga
     1321 atccggcgca ttcaccgagg gcgccgtgtc ggcgacctcg aagtcaccgc tctcgattcc
     1381 cagcaggttg cggagcacgc ggaccgccag cgcattcgcg ttgcgggcgc ggacgagttc
     1441 ctcgcgcgcc tgcgccagcc gaacctcgat gtccagcaca tcggccttca gcagtgtgcc
     1501 gccgtcgagg cgcttcttcg ccaccgccag gctcccctca aacgaattga ctcccgcttc
     1561 ggcggcccgg atgaatcgcc gcgtcttgag caccgtgtaa aaggcgcggg acacctcgaa
     1621 gccgagagcg ttgcggacgg cctcattgtc ctgccgggcg gcgtcggtat tggctttagc
     1681 cgccttgcga gtcgccgtgt tcttgccgcc cgcgtagagc ggaacggtga ccaggccccg
     1741 ggcgttgagg tcatccacgc tcggcacatc attgaagttg agcgacgaac tgtaggcgcg
     1801 ctggctgagg atgctgccga aggccagcat cgggttgtcg ctgccggtgt agctggactg
     1861 gaattgcacg cggggccaga aggcggcgtt agcctgttcc agcccggcct gtgcgctcac
     1921 aatgcgctgc tgagccaggc gcgcgtccgg attggaggcc agcgcctgct ccaaagcgcg
     1981 ctccagcgtc cacggctcgg cgcacaaggc cgacagggac cacgaaagca acagccctag
     2041 aattcccagt tgcttcgtca tttcgcgcag caggagtcgc cggccttttc cgacccgcag
     2101 caactgccgc ccatgccggc tttcctgaga atagtctcca aggggcagaa gttggtgaac
     2161 gaggattgga gcagattaaa gcccacgaac gccgtgaacc aaagccagta cgggctgtgg
     2221 tagtgtgcca gcactaaact aacgaggatg aaggccccgg caaaccgacg gatgatcaat
     2281 tgcattttca taagagctga ctcgcttggt tccaaggtca atgaatactg ctagccacca
     2341 gcgccccgac tagaccgccg tagagggtgc tgatgaacgg gttgctggtc aacgggcagg
     2401 tgccggtcga gcagcccaca aacttgtaaa aggcaaaacc caacccgccg cccaccacta
     2461 ccccgataac gatacgtaga atcataatct attgtctttc gagttccaac ccataccgag
     2521 ccgcgaacgg cccggaagtt acaagcaatc cggttcccga gcggccagcc atgcctgtgt
     2581 gttccctcgc catttcctgg cttcggatgg ctttgtccgg tctttggatg ttcgaggttg
     2641 gatgttggtt gttggatgtt caaagcgcat tacgtttgaa tagcggccaa accaacgctg
     2701 tcgaaccgac ggctcggcta ggtgtgttcg cggggatggc ttcggggccc ggcggagatt
     2761 acaggcgcag aacaagcgta gaatacgaag agaatacgaa gagaatacga ag
//
LOCUS       DATCGS010000228         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_215812, whole genome shotgun sequence.
ACCESSION   DATCGS010000228 DATCGS010000000
VERSION     DATCGS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215812"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VIL39_04615"
     CDS             complement(<1..257)
                     /locus_tag="VIL39_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY5480740.1"
                     /translation="MKTRKLAPIHPGEILREEFMKPHGLSQNALARALNVPPRRINEI
                     VLEKRGITADTALRLARYFGTTAELWTGLQADYDLRLARYE"
     gene            complement(330..1928)
                     /locus_tag="VIL39_04620"
     CDS             complement(330..1928)
                     /locus_tag="VIL39_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5480741.1"
                     /translation="MHSANPEVAQAPIHFNFATDVIDRWAQERPEAPALWCVNATTGA
                     EQKFTFRQVATLSCQAADFLRSSGVRRGDRVLIMLPRVPQWWIAMLGLIRLGAVPVPG
                     TLLLTARDVAYRLGSARISAVLTNQDGVAKVGGFEGTRLLVGGGRSGWLDFNRGVREA
                     STGFRGERTRSDDPGIIYFTSATTGDPKMVLHTQASYGLGHRLTGQFWLDLRPDDVHW
                     NLSDLGWGKAAWSSFYGPWQMGACIFALDTPGKFDPAGALKTLAAFPITTWCAPPTAL
                     RLIVRQDLSGWHFPHLRHCVSAGEPLNPEVLSLWREATGLTLYEAYGQTETVVLVGNF
                     RALGHEVRPGSMGKATPGFTVALLDEHLREVPAGEEGEVAVRVKPERPLGLFREYWLN
                     PNEMAAHFRGDWYLTGDRATRDADGYFWFIGRKDDVIKSSGYRIGPFEVESALLEHPV
                     VLEAAVVGQPDPLRGQIVKAYVVLRPGIKDAPELKSELQTHCKKLIAAYKYPREVEFV
                     KELPKTTNGKTRHMKLRQNRAAKA"
     gene            complement(2003..3367)
                     /locus_tag="VIL39_04625"
     CDS             complement(2003..3367)
                     /locus_tag="VIL39_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5480742.1"
                     /translation="MLLTASSAFAGTGAPTVESRLAELEAKVNSTSGINNGDNAWLLV
                     SSALVLMMSAPGLILFYGGLVRQKNVLGTMMHSLVLMGLVSVLWVLFGYSMVFGQGNA
                     FCGNPWQHFLLRGVGSAPNPDYGATVPEESFMLFQMMFAIITPALISGAVAERMKFSA
                     WLLFTVLWVTFVYFPLAHMVWGKGGWFNWALGGKIPVLDFAGGTVVHISSGVSALVCA
                     LVLGKRLGYPRAPMPPHNVVLSLIGAGLLWVGWFGFNAGSAVNAGKLATSAFAATHFS
                     ASAAALSWAFCEWWQRGRPSVLGVASGMVAGLATITPASGFVSIPAALMIGLAAGAAC
                     YFTVTHLKSRFGYDDSLDVFGVHGVGSTLGMLMLGFLASTLINPAIAVTWKQNGQLVS
                     LAGGAGQFVNQFKGVAFTMLFAGTATFVILKVVNALVGLRVSEEDETLGLDLTQHGES
                     AYND"
BASE COUNT          696 a         1166 c         1031 g          602 t
ORIGIN      
        1 ttctcgtagc gggccagccg caagtcgtag tcggcctgga gccccgtcca aagctcagcc
       61 gtcgtgccga aatagcgcgc cagccgcaac gccgtgtcgg ccgtgatgcc tcgcttctcc
      121 aaaacgattt cgttgatccg ccgcggcggc acattcaatg cgcgtgccag tgcattctgg
      181 gaaaggccgt gcggtttcat aaactcctcg cggagaatct cacccgggtg aatcggggct
      241 agttttcttg tcttcatacc ttaatgataa tccgctattt ctacgcccag ttaaacgagc
      301 gggccatccc ggccatgctc catccgtttt caggcttttg cggcgcggtt ctgcctcagt
      361 ttcatgtgcc gggtcttgcc gttggtggtc ttgggcagtt ccttcacgaa ttcgacctcg
      421 cgcggatact tgtatgcggc aatgagtttc ttgcagtgcg tctgcagttc cgacttcaat
      481 tccggggcgt ctttgatgcc tggccgtagc accacatagg ccttcacaat ctggccgcgc
      541 agcggatccg gctggccaac caccgccgcc tccagcacca ccggatgttc cagcagcgcg
      601 ctttccactt caaacggccc gatacggtag ccggaactct tgatcacgtc gtccttgcgc
      661 ccgataaacc agaaatagcc gtcggcgtcg cgtgtggcgc ggtcacccgt cagataccag
      721 tcgccacgaa aatgggccgc catttcgttt gggtttagcc aatactccct gaacaggccg
      781 agagggcgtt ctggcttcac gcgcacggct acttcgcctt cctcgcccgc cggcacctcg
      841 cgcaggtgct catccagcag cgccacggtg aaacctggtg tggccttgcc catcgaaccg
      901 ggccgcacct catggcccag cgcacgaaag ttaccgacga gcacgacggt ttccgtctgg
      961 ccgtaggctt cgtatagcgt cagaccggtt gcctcccgcc agagactcag cacctccggg
     1021 ttgagcggtt cgcccgcgct gacgcaatgc cgcagatgcg gaaaatgcca gcccgacaaa
     1081 tcctgccgca cgatcaaccg cagcgccgtg ggcggcgcgc accaggtcgt aatggggaac
     1141 gcggccaggg tcttgagcgc ccctgcgggg tcaaacttgc ctggcgtatc cagcgcaaat
     1201 atacatgcgc ccatctgcca ggggccatag aagctcgacc acgccgcctt gccccagccc
     1261 aagtcggaca ggttccaatg gacatcgtcg ggccgcaaat ccagccagaa ctgccctgtc
     1321 agacgatgtc ccagcccata gctggcctgc gtgtgcagca ccatcttcgg atcgccggtc
     1381 gtggcgctgg tgaaatagat aatccccgga tcatcgctac gcgtccgctc gccgcggaat
     1441 ccagtgctgg cctcgcgcac gccgcggttg aaatcaagcc atcctgaccg cccgcccccc
     1501 accagcaagc gcgtgccctc gaagccgcca accttcgcca ctccgtcctg gttggtgagg
     1561 accgcgctaa tccgagcgct ccccagccgg taggcgacgt cccgcgcggt gagcagcagc
     1621 gtgccgggga ccggcaccgc ccccaaccgg atgaggccga gcatggcaat ccaccattgc
     1681 ggcactcgtg gcaacatgat caggacgcgg tccccgcgcc ggacgccgct ggagcgcagg
     1741 aagtccgccg cctgacagct cagcgtcgcg acttgcctga aagtgaactt ctgctcggcc
     1801 ccggtcgtgg cattcacgca ccaaagggcg ggggcctccg ggcgttcctg ggcccagcga
     1861 tcaatcacat cggtggcaaa gttgaagtgg atcggcgcct gggccacttc ggggttcgca
     1921 ctgtgcattg cgctaattgg tagctggatt ggcggtcaaa gtaaaggaac cggcctatgg
     1981 gccttctcag acgggtgcgg ggctagtcat tgtaagcgct ttcgccgtgt tgcgtcaggt
     2041 ccagtccgag ggtctcgtct tcttcgctga cgcgcaggcc aaccagggcg ttgacgactt
     2101 tcaggatgac aaaggtggcc gtaccggcga agagcatggt gaacgccact cccttgaact
     2161 ggttcacgaa ctggccggcg ccccccgcga gcgacacaag ctggccattc tgcttccagg
     2221 tgacggctat cgccgggttg atcagcgtgc tggccaggaa tcccagcatc aacatgccca
     2281 gggtgctgcc gaccccatgc acaccgaaga cgtcaagcga gtcgtcatag ccaaacctgg
     2341 acttgaggtg cgtgacggta aagtaacagg ctgctcccgc cgccaggcca atcatcagcg
     2401 ccgccgggat ggagacaaat cccgaggcgg gtgtgatggt agccagcccg gcgaccattc
     2461 cggacgcaac gcccagcacg ctgggcctgc ccctttgcca ccactcgcaa aaggcccaac
     2521 tgagcgcggc agcggaggcc gagaaatgcg tggcggcaaa cgcgctggtg gccagcttgc
     2581 ccgcgttcac cgcgctgccg gcgttaaaac caaaccagcc cacccagagc aaaccagcgc
     2641 cgatgaggct cagcaccaca ttgtgcgggg gcataggcgc gcgcgggtag cccagacgct
     2701 tgcccaagac cagggcacag accagcgccg aaacgcccga gctgatgtgc accaccgtgc
     2761 cgccggcaaa atccagcacc gggattttcc caccgagcgc ccagttgaac catccgccct
     2821 tgccccagac catgtgtgcc agcgggaaat agacgaaggt cacccacagc accgtgaaca
     2881 gcagccaggc gctgaatttc atgcgctccg ccaccgcccc gctgatcaac gcaggggtga
     2941 tgatggcgaa catcatctgg aacagcatga aactctcctc cggcacggtc gccccgtagt
     3001 ccgggttagg cgcggagccg accccgcgca gcaggaagtg ctgccaagga ttgccgcaga
     3061 aggcattgcc ctggccaaag accatgctgt agccgaaaag gacccagagc accgaaacca
     3121 ggcccatcag caccaggctg tgcatcatgg tccccaaaac attcttctgc cgcaccagcc
     3181 cgccgtagaa caggatcagg cccggcgcac tcatcatcaa caccagcgcg gagctgacca
     3241 gcagccaagc gttgtcgccg ttgttgatgc ccgaggtgga gttgaccttg gcttcgagtt
     3301 cagcgagccg ggactccacc gtgggcgccc ccgtccctgc aaaggcagag gaggccgtca
     3361 gaagcaacag caggagcccc aatacagaga gtcgcgaact aattcgccgg ttaaatatgc
     3421 agcgcatggg cgcaaagtgg actggaatgt agcgccggag tcaagtgaac ccgtttgaac
     3481 gccaccggct tgaga
//
LOCUS       DATCGS010000229         5339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_417000, whole genome shotgun sequence.
ACCESSION   DATCGS010000229 DATCGS010000000
VERSION     DATCGS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417000"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1763
                     /locus_tag="VIL39_04630"
     CDS             258..1763
                     /locus_tag="VIL39_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480743.1"
                     /translation="MAKDGKFRWSKLVLVLVVLGAAVGGVMYFKNDHSEAPQYQTTAV
                     TRSDLTQAVSASGTLNPVLNVQVGSQISGNIAKLFADFNSLVKSNQVVAQLDPAIFQA
                     SVHQAEGDLANANASLELQRAETTRSETLFKNKLISQSDYDKAIATLHQAEAQLKIKQ
                     ASLERSQLDLAHCTIYAPVDGIVISRNVDVGQTVAASMNAPVLFQVANDLAKMQIDTN
                     VSEADVGTVEEGQNVSFTVDAYPGRTFVGRVVQIRNSPVTVQNVVTYDTVIGVTNSDF
                     KLKPGMTATVAITAAQRTNVLKISNSALRFKPTDPSTNQTLVARLLAKVGLGKEPKTA
                     ATNAVQIAKAVGTNKVETAGNGSPPLTGNEPPEELARRVREMRDRGEEPTPEIRAKLR
                     ELYQSGALQRSGQGGRSAGGGSGSRPPSAPAWRNIYVLTTTTPPGGGDPVSKPEAVRV
                     RLGITDSYYTEVLDGLKEGDMVITGVKLPAAQAATPAPAGQSPFGGPGRMR"
     gene            1770..3089
                     /locus_tag="VIL39_04635"
     CDS             1770..3089
                     /locus_tag="VIL39_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480744.1"
                     /translation="MPPQPENAAANVTTVPEEVTGQPRRVVAGVVPWRQTFAALKHRN
                     FRLFFFGQLISLTGTWMQNTAQSWLVYQLTGSKLLLGIVAAVGSAPMLLFSLWGGSVA
                     DRHPKRVVLLWTQSGMMVLAFVFAALVWSKLVQPWHILVLAALGGVAMAFDMPVRQAF
                     MVEMSSREDLMNALSLNSSIVNGARVAGPAVAGILIAQAGLTACFVLNGLSFVAVLAG
                     LLLMRLPRFIPPAQPESNWQHALDGFAYVAKHRRVRTLLLLFAIVGVFGWSYSVLLPA
                     FAADVLHVGSRGYGMLLSANGIGALLGALTVATYGDLLHRRLMVFGGLGLFSLMLLLL
                     AVTRNYYLALLFLAIAGWGMLLYFSTTNTLIQLGVADGMRGRVMGIWALVFGGMMPVG
                     GMEAGALSHWLSVQAAVAIGAVVCGAAALMTWLYVRKNSPSPGTLAD"
     gene            3240..4001
                     /locus_tag="VIL39_04640"
     CDS             3240..4001
                     /locus_tag="VIL39_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5480745.1"
                     /translation="MDTVIQLQDIHKTYHTGEVDVHAVRGVSLDIAQGEFVALMGASG
                     SGKSTMMNIIGCLDRPTGGKYLLDGIDVSRLSRDELADIRNQKLGFVFQGFNLLSRTS
                     ALENVELPMLYARRRADGHSQRERALKALDTVGLADRADHKPNQLSGGQQQRVAIARA
                     LSNEPALLLADEPTGNLDTRTSVDIMGVFQKLNDQGMTIVMVTHELDVAQYTKRMIVM
                     RDGKVVSDTPVAKRLIAETELRRLQEEQKAAQLTS"
     gene            4099..5316
                     /locus_tag="VIL39_04645"
     CDS             4099..5316
                     /locus_tag="VIL39_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480746.1"
                     /translation="MRYFVIFKAAFRALRRNKMRTVLTMLGIIIGVGAVIAMVGIGNG
                     AKAQVQARIAALGQNVIMVFAGSVNRGGVYSGSGGAGTLTVEDALAMEKEVPGVSAVS
                     PEVRSGAQIMAGNNNWSTSVMGEGVGYLGIRQWDIEDGTMFTDADVRAAAKVCILGKT
                     TADHLFPEDDPVGKTIRIKNVPMKVLGVLRAKGASMFGSDQDDTVVVPYTTGMKRFAG
                     VTTLRMINVQAASPEQMTEVQNGIAELLRQRHRIQPGRDDDFMMRNQQEIAEAMSATT
                     DVMTALLAAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGARAHDILLQFLIEA
                     VVLSSTGGMLGILCGVGGAKLITMIKNWPTLVSANSIIIAFAFSAVVGVFFGFYPARK
                     ASQLDPIDALRYE"
BASE COUNT          987 a         1663 c         1707 g          982 t
ORIGIN      
        1 agcgaagcgc gacgccgctt ttcctgacag cagtagtccg ctgatgcagc aggagtgcca
       61 cccgcagcca aagcggcgtc gcgcttcgct tgccgccgca ctccacatcc gtggcgccgg
      121 gacatcccgg cgactttgtc aatcgccctc ccgcccgggc cgcaccaatt cccactatcg
      181 acaggggcaa gaaatcaagt aacctgtcaa tggctgcatc tgtcttatta aagacaaccg
      241 gtccggttga tacaattatg gcaaaagacg gcaagttccg ctggtcgaag ttggttttgg
      301 tgttggtagt gctcggcgcc gcggtcggcg gcgtgatgta tttcaagaac gatcactctg
      361 aggcgccgca gtatcaaact acggccgtca cccgcagtga tctgacccag gcagtgagcg
      421 ccagcgggac gttgaacccg gtcctcaacg tgcaggtcgg gagccagatt tccggcaaca
      481 tcgccaagct cttcgccgac ttcaattccc tggtcaaatc caaccaggtc gtcgcccaac
      541 tggacccggc catcttccag gccagcgtgc accaggccga aggggatctg gctaatgcca
      601 atgcctcgct cgaactgcaa cgagccgaga ccacgcgctc ggaaacgctg ttcaagaaca
      661 agctgatctc ccagtccgac tatgacaaag caatcgccac cctgcaccag gcggaggcgc
      721 agttgaagat aaagcaagcc tcgctggaac gctcgcagtt ggatttggcg cactgcacca
      781 tttacgcgcc ggtggacggc atcgtcatct cgcgcaacgt ggatgtgggc caaaccgtcg
      841 cggcgagcat gaacgcgccg gtgctgttcc aggtggccaa cgacctcgcc aagatgcaga
      901 ttgatacgaa cgtgtccgag gccgatgtgg gcacggtgga ggaggggcag aatgtgagtt
      961 tcaccgtgga tgcgtatccc ggccgcacct ttgtcggccg ggtggtgcag atccgcaact
     1021 cgcccgtcac agtgcaaaac gtcgttacgt acgacaccgt catcggcgtc accaattccg
     1081 atttcaagct taaacccggg atgaccgcga cggtcgcgat caccgccgcc cagcgcacca
     1141 acgtgttgaa gatctcgaac agcgccctcc gcttcaagcc gacggacccg tccaccaacc
     1201 agacgctggt ggcgcggctg ttggcgaaag ttggtttggg taaggagccg aagaccgcgg
     1261 cgacaaatgc cgtccagatc gcgaaggccg tcggcaccaa caaggtcgag acggcgggca
     1321 acggttcccc gcccctcact ggcaacgagc cgcccgagga gttggcgcgc cgggtgcgcg
     1381 agatgcggga ccggggagaa gagccgacgc cggagattcg cgccaaactg cgggagttgt
     1441 accagagcgg cgcgttgcag cggtcgggac agggtggccg atctgcgggc ggcgggagtg
     1501 gttcgcggcc tccatcagcg ccggcctggc gcaatattta cgttctgacc accacgacgc
     1561 cgcccggcgg cggtgaccct gtctccaagc ctgaagcggt gcgcgtgagg ctcggcatca
     1621 ccgacagcta ctacaccgag gtcctcgatg gattgaagga aggggacatg gtcatcaccg
     1681 gggtcaagct gcccgcggcc caggcggcca ctccggcgcc cgcgggtcag agtccgtttg
     1741 gcgggccggg gcgaatgcgc taatgagcca tgccgcccca accggagaac gcggccgcca
     1801 acgtcaccac ggtgcccgag gaggtaacgg gtcagccccg gcgggtggtg gccggggtgg
     1861 tgccgtggcg gcaaacgttc gccgcgctca agcaccgcaa ctttcggctc ttcttcttcg
     1921 gccagttaat ttcgctaact ggcacgtgga tgcagaacac ggcccagagc tggctggtct
     1981 atcagctcac agggtccaag ctcctgctcg gcatcgtcgc ggcggtgggt tccgcgccga
     2041 tgctgctgtt ttccctatgg ggtggatcgg tggcggaccg gcatccgaag cgggttgtgc
     2101 tgttgtggac acagagcggc atgatggtgc tggcgtttgt gttcgcggcg ctggtttgga
     2161 gcaagctggt gcagccgtgg catatcctgg tgctggcggc gctcggggga gtagcgatgg
     2221 ccttcgacat gccggtgcgc caagccttca tggtcgagat gagcagtcgc gaagacctga
     2281 tgaatgcgct gtcgctcaac agctctatcg tcaatggcgc gcgcgtggcc gggccggcgg
     2341 tggcggggat actcatcgcg caggcgggcc tgacggcgtg ctttgtcttg aacgggctga
     2401 gtttcgtggc ggtgctggcg ggtctgttgc tgatgcggtt gccccggttc ataccgcccg
     2461 cacaaccgga atccaactgg caacatgccc tggacgggtt tgcctacgtg gcgaagcatc
     2521 gccgggtgcg cacgctcttg ctgctgtttg ccatcgtcgg cgtgttcggc tggtcgtatt
     2581 cggtgctgtt gcctgcgttt gccgcggacg tgctgcatgt cggcagccgc ggatatggga
     2641 tgttgctcag cgccaacggc atcggggcac tcctcggggc gctgacggtc gcgacttatg
     2701 gcgacctcct gcaccggcgg ttgatggtgt tcggcgggtt ggggcttttc tcgctgatgc
     2761 tgctgttgct ggcggtgacg cggaattact acctggcgct gctctttctg gcgatcgccg
     2821 gctggggcat gttgctttac ttttccacca ccaacacctt gatccagttg ggtgtcgccg
     2881 acggcatgcg cgggcgcgtg atgggcattt gggcgctggt gttcggcggc atgatgccgg
     2941 tgggcggtat ggaggcgggc gccctgtcgc actggctcag cgtgcaggca gcggtggcca
     3001 tcggcgcggt ggtgtgcggc gctgcagccc tgatgacgtg gttgtacgtg cgcaagaatt
     3061 cgcccagccc cggcacgctg gcggattgaa agggtcaaat ggtggcatgg ttgcataagt
     3121 tgcatgagtt acatgggtat tgcgtgaagt cgcccgctgt cactatgcaa cgatgcaact
     3181 tatgtaaccc gtgtaactca cgtaactcat gtaacgcctt aacattgtaa cgcccccaga
     3241 tggacaccgt cattcaactt caggacatcc acaagaccta ccacaccggc gaggtggatg
     3301 tgcatgccgt gcgcggcgta tcactggata tcgcccaggg cgagtttgtc gcgctgatgg
     3361 gcgcgagcgg gtccggcaag tccaccatga tgaacatcat cggctgcctc gaccggccaa
     3421 cgggggggaa gtatttgctg gatggcattg acgtctcgcg gctcagccgt gacgagctgg
     3481 ccgacatccg caaccaaaag cttggcttcg tcttccaggg cttcaacctg ctctcccgca
     3541 cttccgcgct ggagaatgtc gaactgccga tgctctatgc ccgccggcgc gcggacggac
     3601 atagccagcg tgagcgggcc ttgaaggcgc tggacacggt cggcctggct gaccgcgcgg
     3661 accacaagcc gaaccagctt tccggcggcc agcagcagcg cgtggcgatt gcccgcgcac
     3721 tgtccaacga gccggccctg ctcctcgccg acgagccgac cggcaatctc gacacccgca
     3781 ccagcgtgga catcatgggc gtgttccaga agctcaacga tcagggcatg accatcgtca
     3841 tggtgaccca tgagctggac gttgcccagt acaccaagcg gatgatcgtc atgcgcgacg
     3901 gcaaagtggt cagcgacacc ccggtagcca aacgcctgat cgccgaaacc gagctgcgcc
     3961 gattgcagga ggaacagaag gccgcgcagt tgaccagttg aacgcgagga caggcacaat
     4021 gacgactcca acgccaattc ggatttcagc cttcgggctt ccttcggatc tcggatttcg
     4081 gccttcggat ttctagtcat gcgctacttc gtcatcttca aagccgcctt ccgcgcgctg
     4141 cgccgcaaca agatgcggac cgtcctcacc atgctgggca tcatcatcgg cgtcggcgcg
     4201 gtcatcgcca tggtcggcat cggcaacggc gccaaggccc aggttcaggc ccgcatcgcc
     4261 gcgctgggcc aaaacgtgat catggtattc gccggcagcg tcaaccgcgg cggcgtttac
     4321 agcggttccg gcggcgccgg cacgctgacg gtggaagacg ccctcgcgat ggagaaagaa
     4381 gtgcccggcg tctccgccgt cagcccggaa gttcgcagcg gcgcgcaaat tatggcgggc
     4441 aacaacaatt ggagcacatc cgtcatgggc gagggagtcg gttaccttgg gattcgccaa
     4501 tgggacatcg aggacgggac catgttcacg gacgccgacg tgcgcgccgc cgccaaggtc
     4561 tgcatcctcg gcaagaccac cgccgaccac ctcttcccgg aggacgaccc agtgggcaag
     4621 accatccgca tcaagaacgt gcccatgaag gtcctcggcg tgctccgggc caagggtgct
     4681 tccatgtttg gttccgacca ggatgacacc gtcgtcgtgc cttacacgac cggcatgaag
     4741 cggtttgccg gcgtgaccac gctccgcatg atcaacgtgc aggcggccag ccccgagcaa
     4801 atgaccgagg tgcaaaacgg catcgccgaa ctgctccgcc agcgccatcg catccagccg
     4861 ggccgcgatg acgacttcat gatgcgcaac cagcaggaaa tcgccgaagc catgagcgcc
     4921 accactgatg tcatgaccgc gctgctggcc gccatcgcca gcgtctctct gctggtgggc
     4981 ggcatcggca tcatgaacat catgctcgtc agcgtgaccg agcggacgcg ggaaatcggc
     5041 atccgcatgg ccgtcggcgc gcgcgcccac gacatcctcc tgcaattcct gatcgaagcc
     5101 gtcgtgctca gttccaccgg cggcatgctg gggatattgt gcggtgtcgg cggcgccaag
     5161 ctcatcacca tgatcaagaa ctggcctacg ctcgtctccg ccaactccat catcatcgcc
     5221 ttcgccttca gcgccgtcgt aggggtgttc ttcggcttct atcccgcccg caaagcctca
     5281 cagcttgatc ccattgacgc gctgcggtat gagtgaagcg gcacaggctg ttacagaaa
//
LOCUS       DATCGS010000230        12579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_268116, whole genome shotgun sequence.
ACCESSION   DATCGS010000230 DATCGS010000000
VERSION     DATCGS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268116"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VIL39_04650"
     CDS             <1..240
                     /locus_tag="VIL39_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480747.1"
                     /translation="FQLETTKTPLFASKAARELALPSGGRFGLRRDFGLRFGLGVDLL
                     PLQIGIPTFSFLSFVVLLAHNYLYITNSLRLFSAL"
     gene            237..773
                     /locus_tag="VIL39_04655"
     CDS             237..773
                     /locus_tag="VIL39_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480748.1"
                     /translation="MKIRWSRFNIYLTVALVAAVVCGCRTASESKAKKQLSTLRLHLE
                     TGRDAAGATKAVPVYRRQPFMVNVEKTTCLNESNVSEARVVDVVGGFALRIQYDHAGT
                     ALLEQASTANRGKRIAVFSQFGENLKDYRWLAAPVISQRITDGVFIFTPDATREEAEE
                     IALGLNNTAKKTHTWIDK"
     gene            758..1759
                     /locus_tag="VIL39_04660"
     CDS             758..1759
                     /locus_tag="VIL39_04660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5480749.1"
                     /translation="MDRQIVLSSFLIAFAAQAQTFQIPTANHALFEKGGEDRFFVGTA
                     GKTWTTGTFGCVRTGGWQMHEGLDIRCLQRDKRGEPTDPILATADGTVAYISMRPSLS
                     NYGNYLILRHHVEGLEIYSLYAHLHEVRHDLKVGQAVKAGEPVATMGRTANTRETISK
                     DRAHVHFELNLLVNDRFAAWHKQSVPGQRNDHGEWNGQNLFGIDPKPILLGQHEQGAK
                     FSLLSLLQNQTELCRVLVRKTDFPWLKRYAPLVRPKPLTEKESIAGYEIALNFNGVAF
                     ELIPRTASEIKGKAKFQLISVNEAEYARNPGRRLVVKRGSRWELTSHGLNLLELLTY"
     gene            complement(1872..3134)
                     /locus_tag="VIL39_04665"
     CDS             complement(1872..3134)
                     /locus_tag="VIL39_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY5480750.1"
                     /translation="MDYSPAPKRARLQSLAEELASFREFGQATRKLVTTFHAADDQTR
                     EVPTFVNEFWTAKQRQASSLQEISYRACFKPQLPRFFIERLTQPGEVVYDPFMGRGTT
                     VLEAALLGRVPCGCDINPLSVVLTRPRLRPPRLEDVAQRLKQIDFTSSDDFPEDLLVF
                     FHPDTLRAIASLKKYLLARRAANLLDAADDWICLLALNRLTGHSSGFFSVYTMPPNQA
                     VSVKSQQKINARRNQTPPSRDVVGIILKKTRILLRDCDAATRQVLAGVAPRAVLLTKP
                     AACTPEIPSQSVALVVTSPPFLDVVDYATDNWLRCWFLGIDAKSVKLTVPKKLYAWQT
                     AMTAVFHELHRILKPGGHIAFEVGEVHGGKTKLEETVLPCGVAAGLEPMLVLINDQLF
                     TKTANCWGVDNMAKGTNTNRIVLFRRKP"
     gene            3426..3689
                     /gene="rpsP"
                     /locus_tag="VIL39_04670"
     CDS             3426..3689
                     /gene="rpsP"
                     /locus_tag="VIL39_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY5480751.1"
                     /translation="MAVKIRLKRVGAKNKPAFRIVVADGRSPRDGRFIEELGTYLPRN
                     KGNNVTMDLDRAKYWLSKGAQPSETVASFIKKAIKAAMAGAAA"
     gene            3706..3951
                     /locus_tag="VIL39_04675"
     CDS             3706..3951
                     /locus_tag="VIL39_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY5480752.1"
                     /translation="MQAFLEYIVKGLVQNPEAVTITPVEREGTTVYELRLDPPDVGRI
                     IGRQGMTINAIRSLLLAGSAKKGLRCTLEIVEDKPAT"
     gene            4041..4706
                     /gene="trmD"
                     /locus_tag="VIL39_04680"
     CDS             4041..4706
                     /gene="trmD"
                     /locus_tag="VIL39_04680"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY5480753.1"
                     /translation="MKVDVLTLFPAMFAGPLDESIIKRARESGRLDLAIHDLRDYAHD
                     RHKKVDDRPFGGGPGMLLKPEPIFEAVESLARESTRVILLSPSGRQFSQAIACELAGL
                     EHLLMVSGHYEGFDERVREQLADDELSIGDYVLTNGALPVMVIIDAVTRLLPGVLGDE
                     DSARDDSFSQGLLEYPQYTRPAEFRGTKVPEVLLSGNHAEIARWRAEQARLRTRERRP
                     DLL"
     gene            4800..5192
                     /gene="rplS"
                     /locus_tag="VIL39_04685"
     CDS             4800..5192
                     /gene="rplS"
                     /locus_tag="VIL39_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY5480754.1"
                     /translation="MNQALLDTIESEQLRKTATDFGVGDTVRVHTKVVEGDKERIQIF
                     TGVVIGRRGHGLNEMFTVRRISYGEGVERVFPLHSPRVDKVEVERKGSVRRAKLTYLR
                     HRVGKGATLVKERAGRVTASAAPKAGQS"
     gene            complement(5303..6574)
                     /gene="hisS"
                     /locus_tag="VIL39_04690"
     CDS             complement(5303..6574)
                     /gene="hisS"
                     /locus_tag="VIL39_04690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5480755.1"
                     /translation="MERLPGFRDFYPEPLPKPDLWSADARQYIFDKWRGMARRYGFRE
                     YDGPPLEPLELFTTKSGAEIVAQLYNFKDKGEREVSLRPEMTPTLARMVAAYERGYKK
                     PFKWFALPQLFRYERQQKGRLREHFQFNADIIGESDPAADAELIALLIDTLRSFGLTE
                     KDFVIRLSSRNAWQDFFRRHQGDAAQEYAFYQIIDKLEREAPEQSDEKLRRTGFSLAE
                     VSSFIEQGTPTAELEAILKNLAARGLNDYVKIDYRVIRGLAYYTGVVFEAFDRKGEFR
                     AIAGGGRYDNLIKLISGGKVDLPSLGFGMGDVVLLEILKARALLPQFDAALDAFCLIE
                     DETLRGESLKLIHGLRAAGLAVDYSLTPAKSDKQFKRAQELKAAHTIKIERHEAGELL
                     AKMKNLKTRQETVLPPTQAAARLCESRAAGV"
     gene            complement(6676..7008)
                     /locus_tag="VIL39_04695"
     CDS             complement(6676..7008)
                     /locus_tag="VIL39_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY5480756.1"
                     /translation="MTLTKRGLVLRICEDTDLAQQQVFDIVQRTLDHIIKALAQGEKV
                     ELRNFGIFSVRVRKARTGRNPSAPGTEVPIPERSVVKFKAGREMRLSVRKLTPKPAAK
                     PPTKPPKA"
     gene            complement(7171..8085)
                     /locus_tag="VIL39_04700"
     CDS             complement(7171..8085)
                     /locus_tag="VIL39_04700"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY5480757.1"
                     /translation="MNLSVKIGTLTLQNPVTVASGTFGYGVEYSQLINLNQLGAVVVK
                     GIRLGPVRGNPTPRTVEVTSGLINAIGLQGPGVDGFIRKYWPFLKALKVPTIINIWGT
                     TVEEYAEVARRFDALGGVGALELNVSCPNIKEGGAQFGTDVTLLSQVVGACRKVTKLP
                     LITKLSPNVVSIAPYAKAAEAAGSDALSIMNSFPAMAIDIETRRPRLANVTGGLTGPC
                     IKPIAIKLVWEAAKAVRIPIIGMGGIQSTADAIEFLMAGATAVAVGTANFYEPQTALQ
                     VIAGLRQFMQQNRIKDLRELIGSVQTRK"
     gene            complement(8082..8867)
                     /locus_tag="VIL39_04705"
     CDS             complement(8082..8867)
                     /locus_tag="VIL39_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY5480758.1"
                     /translation="MVEQTVQIVSNEREADLYFRLVVRAPGIAPLVQPGQFAHVRILP
                     LREALLRRPFSIFQVSGSTLSILYKTIGKGTEVLSRMRPGEELSMIGPLGHGFTVPPP
                     SGETPLLVAGGYGMAALYLLAQRSPQKGIVFVGGRRRVDILCEEEFRALGWEVRVTTE
                     DGSYGEKGLLTEALLTELKRRTPGRKLFACGPSPMLKAVGRLAAEFDLQAELSLDEHM
                     GCGIGVCLACVVPIKAGDSWEYRRTCTEGPVFDSRQVAWEAAK"
     gene            complement(9079..11094)
                     /gene="shc"
                     /locus_tag="VIL39_04710"
     CDS             complement(9079..11094)
                     /gene="shc"
                     /locus_tag="VIL39_04710"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY5480759.1"
                     /translation="MDAVSLREGRIEGNPRQQDLGLKPQIELEAAIERSQSYLLSQQK
                     PEGYWVGELMVDATLVADTIAYHHWNGKVDPQWHRKAVNHIFSMQLSDGGWNIYYGGP
                     AEVNATIKCYLALKLAGVPVTDPRMLRAREVALNLGGVPRMNTFSKLYLALLGLYSWE
                     YVPTIPCEVILIGKWFYVNFNEMSSWSRSMLVPLAIINHYRPTRLLKKNIDIDELYPE
                     GIHERDLALAPDPERITWRNFFLWLDRVHKLAEWFAQHHIHPFRKRALRKAEQWMLER
                     FEGSDGLAAIFPAMLNSLIALKALGYPDDHPLVVRAERELKKLEHESEQSVHIEPCLS
                     PVWDTAIVNMCLSESGVPANHPALKRATEWLMAREIRFRGDWQYKNPAKVEPSGWVFE
                     FENKWNSDVDDTAMVLLALRKVPTDDRQKRDECFQRGLKWMMTFQCRDGGWAAFDKDC
                     TKGVLEKVPFADHNAMLDPECADITARILELLGYEGWSVEHPQVQKALRFIREHQEED
                     GSWYGRWGVNYVYGTWQVLRGLRALNLDMNEPWLTKARDWLESVQHEDGGWGERCNTY
                     DDPVFKGQGPSTASQTAWAVMGLCTFDDPNRPSVRRGIEYLIRMQNADGSWTEHETTG
                     TGFPKVYYLKYDMYRNTWPLLAMATCRDLLTRAVAKRDGEAIGATVP"
     gene            complement(11238..12245)
                     /locus_tag="VIL39_04715"
     CDS             complement(11238..12245)
                     /locus_tag="VIL39_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5480760.1"
                     /translation="MDTLAGMIIDRRGFLKLAAAASVGTLVGGARVGGAEPTASTEVA
                     RSGKHPIIWGNLLHLSTNMWCDWDAPELKGDGSIYRPELRFDRSLWNDLLKKMQQAGM
                     NMVVNDLGDGVKYKSHPEIAIKGAWSTAELRGELKRMRALGLEPIPKLNFSTTHDAWL
                     GKYARCVSTDTYYAVCRDLIAEVMQLFDRPRFFHLGMDEEDYGNQKYYEHVVIRQYDA
                     WWRDFLLLVAQVEKSGARAWIWSDYVWHHPELFYRKMPKSVVQSNWYYDAKFDPQKAV
                     AKAFRELDQHGYDQIPTGSNYYNSTNFELLAEECRRSLGAGHLLGFLQTPWKPTVEKY
                     R"
     gene            12308..12490
                     /locus_tag="VIL39_04720"
     CDS             12308..12490
                     /locus_tag="VIL39_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY5480761.1"
                     /translation="MKGYPFETPLPAGQPISGVVLADHARSLDWNSRHAELICQLPAE
                     VVQDIAAKIIALVAED"
BASE COUNT         2462 a         3767 c         3849 g         2501 t
ORIGIN      
        1 ttccagcttg aaactactaa aaccccgctt tttgcgtcaa aagccgcccg tgaattggcc
       61 ctgccgtcag gcggtcgctt tggtcttagg cgcgactttg ggctgcgctt tggccttgga
      121 gttgaccttc ttccgcttca aataggcatt ccgacgtttt cgtttctcag ctttgttgta
      181 ttgcttgctc ataactatct ttacattacc aactcattgc gattgtttag cgcactatga
      241 agattcgctg gagtcgattc aatatctatt tgacggtggc gctggtcgca gcggtggttt
      301 gcggatgccg aaccgccagc gaaagcaagg ccaagaaaca gctcagcacg ttgcgcctgc
      361 atcttgaaac gggccgagat gcagccgggg ccaccaaagc ggtccccgtt tatcgccggc
      421 agccgtttat ggtcaacgtg gagaaaacaa cttgcctcaa cgaatccaat gtgtccgagg
      481 ccagagttgt cgatgtggtg ggcgggtttg ctctgcgcat ccagtacgac catgcgggca
      541 cggcgctgct cgaacaggcc tccaccgcga atcgcgggaa aaggatcgcc gtgttcagcc
      601 agttcggcga gaacctgaag gactatcgct ggctggctgc tcctgtaatc tcccagcgca
      661 tcaccgatgg cgtcttcata ttcacgccgg acgcgacccg cgaggaggcc gaagagatcg
      721 cgctcggcct gaacaacaca gccaagaaaa ctcatacatg gatcgacaaa tagttctttc
      781 atcttttctc atcgcattcg ctgcccaggc ccagaccttt caaatcccca ccgccaatca
      841 cgcgctgttc gagaaaggtg gggaggaccg gttttttgtc ggcaccgccg gcaagacgtg
      901 gacgaccggc acattcggct gtgtgcgcac gggcggctgg cagatgcacg agggcctcga
      961 tatccgctgc ctccagcgcg acaagcgcgg cgaaccaact gatcccattc tcgccaccgc
     1021 cgatggcacg gtcgcttata tcagcatgcg accatcgctg tcgaactatg gcaactacct
     1081 tattctgcgt caccacgtcg aaggcctgga aatctattca ctttacgccc acctgcacga
     1141 agtccgccac gacctgaaag tcgggcaggc cgtcaaggcc ggtgagccgg tcgccaccat
     1201 ggggcgcacc gccaacaccc gggaaaccat ctccaaagac cgcgcgcatg tgcacttcga
     1261 gctgaacctg ctggtcaacg accggttcgc agcctggcac aagcaaagcg ttccaggtca
     1321 gcgtaacgat cacggtgaat ggaatggcca gaatctgttc gggattgatc cgaagcccat
     1381 cctcctggga cagcacgagc aaggcgccaa gttcagcctg ctcagcctcc tgcaaaacca
     1441 gacagaactc tgccgggtcc ttgtgcgcaa gaccgatttc ccttggctca agcgctatgc
     1501 tcccctggtt cgcccgaaac cgcttacgga gaaagagagc atcgccgggt atgaaatcgc
     1561 cctgaacttc aacggcgtcg ccttcgagct cattccgcgc accgcctcgg agatcaaagg
     1621 aaaggcgaaa ttccagctca tttcagtcaa cgaagctgaa tacgccagga accccggccg
     1681 acgtctcgtg gtaaaacgag gcagtcgctg ggaactcacg agccacggtt tgaacctcct
     1741 tgagctattg acatactagc ctcggctggg ccgggtggcg cagcgcgacg acctcggcgc
     1801 atccccgtgt tttcgcagat ttggttcgac atgcccgccg accggcacag ccggggcgcc
     1861 tgcgcgaggg attacggttt gcggcggaac agcacgatgc ggttggtgtt ggtgcctttt
     1921 gccatgttgt ccacgcccca gcagttggcg gtcttggtga atagctggtc gttgatgagc
     1981 acgagcatcg gctccagacc ggcggcgacg ccgcaaggca aaacggtctc ttcgagtttg
     2041 gtcttgccgc cgtgaacttc gccgacctcg aaggcgatgt ggccgccggg tttgagaatg
     2101 cggtgcagtt cgtggaagac ggcagtcatc gcagtctgcc aggcatagag cttcttgggg
     2161 acagtgagct ttacggactt ggcgtcaatg cccaggaacc agcagcgcag ccagttgtcg
     2221 gtggcgtaat ccaccacgtc gaggaagggc ggcgaagtga ctaccagtgc gacggattgc
     2281 gaggggattt caggcgtgca cgcggcgggc ttggtcaaca ggaccgcccg gggcgcaacg
     2341 ccggccagga cctgacgtgt ggcggcgtcg cagtcgcgca gcagaatgcg ggtcttcttc
     2401 aggatgattc cgaccacgtc gcggcttggc ggggtttggt tgcgccgggc attgatcttc
     2461 tgctgcgatt tgacagaaac cgcttggttg ggcggcatcg tatagacgga gaagaacccc
     2521 gacgaatgac cggtcaaacg gttcagcgcg agcaggcaaa tccaatcatc cgccgcgtcg
     2581 agcaaattgg cggcccgccg cgcgagcaga tacttcttga gggaggcgat ggcgcgaagt
     2641 gtgtcggggt gaaagaagac caggaggtct tcggggaagt catcggagga agtgaagtcg
     2701 atttgcttga gccgttgggc gacgtcttcg aggcgcggcg ggcggagccg tggtcgcgtc
     2761 aggacgacgc tcaaggggtt gatgtcacag ccgcagggga cgcgccccag cagcgcggct
     2821 tccaggaccg tagtgccgcg gcccatgaac gggtcataga cgacctcgcc aggctgcgtc
     2881 aggcgctcga tgaagaagcg aggcaactgc ggtttgaagc aggcgcgata ggaaatctcc
     2941 tggagcgagc tggcctggcg ttgtttggcc gtccagaatt cgttgacgaa cgtggggact
     3001 tcgcgtgtct ggtcatcggc ggcgtggaag gtggtgacca gcttgcgggt ggcctggccg
     3061 aactcgcgga atgacgccaa ctcctcggcc aatgattgga gacgggcgcg cttgggcgcg
     3121 ggggaatagt ccaaggacag ctctggagtc tctggaccgg ttgcgactaa agtcttgctg
     3181 ggcaagggcg gcatgtgctt ggctgtaaga agaccattgg cggtgaagtt ccgcaagcgg
     3241 agtttacggg cacggcctgg cttggtcaaa ccgaagcgct ggggtggaac cagccaaaag
     3301 agaaaaacct caaaaacagg gcttgccaaa tcgccagagc aagtctaggc tcgcacctct
     3361 ctttggggaa accctttagg gcgggtgggt ccggatatgg ccgccgtcaa agaaaatgat
     3421 gatttatggc agttaaaatt cgtttgaagc gagtcggggc gaagaacaag cccgcattcc
     3481 gcattgttgt ggctgatggc cgcagtccgc gcgatggccg gttcatcgag gaactgggta
     3541 cctacttgcc gcggaataag ggtaataatg tcacgatgga cctggatcgg gccaagtact
     3601 ggttgagcaa gggtgcgcag ccaagcgaga ccgtcgccag tttcattaag aaagcaatca
     3661 aggcggccat ggctggcgct gcggcctagc gattgatcct ggcttatgca agcgttcctc
     3721 gaatacatcg tcaaaggcct ggtgcaaaac ccggaagcgg tgacaatcac cccggtggaa
     3781 cgcgagggca cgacggttta cgagctgcgc ttggacccgc cggatgtcgg gaggatcatt
     3841 ggccggcagg gcatgacgat taatgcgatc cgttccctcc tgctggccgg cagcgcgaag
     3901 aaggggctgc gctgcacact ggaaatcgtc gaagataagc cggccacgta ggcgcggttg
     3961 gcgttcaagt ttcggcttgt tcccccgggc aacgctcacc ggcggcaaca gtagctaaag
     4021 ctcgaacccc aacggggaag atgaaagttg acgtgctcac gttgtttccg gcgatgttcg
     4081 cggggccgct ggacgaaagt attatcaaac gggcgcggga atcggggcgg ctggatctgg
     4141 cgatccatga tttgcgcgac tacgcgcatg accgccacaa gaaggtcgat gaccggcctt
     4201 ttggcggcgg accgggcatg ttgttgaagc ccgaaccgat ctttgaagca gtggaaagtc
     4261 tggcgcggga gagcacgcgg gtgatcttgt tgtcgccgtc gggccggcag tttagccagg
     4321 ccatcgcgtg cgaactggcc ggactggagc atctgttgat ggtctccggc cactacgaag
     4381 ggtttgacga gcgggtgcgc gagcagttgg cggatgacga gttgtcaatc ggcgattacg
     4441 ttctaaccaa cggcgcgctc ccagtgatgg tgattattga cgcggtgaca cggctgttac
     4501 cgggcgtgct gggcgatgaa gacagtgccc gggacgattc gttcagccag gggctgctcg
     4561 aatatccgca atatacgcgg ccagccgagt ttcgcgggac gaaggtcccg gaagtgctgc
     4621 tctcgggcaa ccatgcggaa atcgcccggt ggcgggcgga acaggcccgg ctgcgaacgc
     4681 gagagcgtcg gccggatctg ctttaacaaa cggtgtgaga gagtgacgga gtggtggagt
     4741 gatgggtgtt cggcccaagc ctctatcccc ctgccctccc tcacccaaac agacatttta
     4801 tgaatcaggc attacttgac acaattgagt cggaacagct tcgcaagacc gccacggact
     4861 tcggggtggg tgatacggtg cgggtgcata cgaaagtggt tgaaggcgac aaagagcgca
     4921 tacagatttt cactggcgtt gtgattggcc gccgcggcca tgggttgaac gagatgttca
     4981 ccgtccgccg catcagctac ggcgaaggcg tcgagcgggt gttccccctg cattcgccgc
     5041 gcgtggataa ggtggaagtg gagcgcaaag gctcggtccg gcgcgccaag ctcacttatc
     5101 tgcgccaccg ggtcggcaag ggcgcaacac tcgttaaaga aagggcaggt cgtgtcacgg
     5161 ccagtgctgc cccgaaggct ggccagtcgt aaccggcagg gcaattgagc tttgcaagcg
     5221 aggcccccgc ctcgcttttt ttgctgccgc ggcgtgagtt cagcaatggg cgccagggac
     5281 ttgcgcctgc agtgctggcg ggtcaaacgc ccgcagcacg actttcacac aatcgagcgg
     5341 cggcctgggt gggcgggaga accgtttcct gccgtgtctt gaggttcttc attttcgcca
     5401 ggagttcccc agcctcgtgg cgctcgatct tgatggtgtg cgcggctttg agttcctgcg
     5461 cgcgcttgaa ctgtttatcc gatttggctg gcgtcagcga gtagtccact gccaagccgg
     5521 cggcacgcag gccatggatc agtttcaggg actcgccgcg caaggtttca tcttcgatca
     5581 ggcaaaaggc gtcgagggcc gcgtcaaatt gtggtagcag tgcgcgcgct ttgaggatct
     5641 ccagcaggac cacgtcgccc atgccgaagc ccagggacgg gagatccacc ttgccgccgc
     5701 tgataagctt tatgaggttg tcgtagcgcc cgccgcctgc aatggcacga aattctcctt
     5761 tgcggtcgaa ggcttcgaaa acgacacctg tgtaataggc caggccgcga atgacgcggt
     5821 aatctatctt cacgtaatcg ttgagcccgc gcgcagcaag attcttcaga atcgcttcca
     5881 gctcggccgt tggcgtgccc tgctcgataa aggacgacac ctcggccagg gagaagcccg
     5941 tccgcctcag cttttcatcg ctctgctccg gcgcctcgcg ttccagcttg tcgatgattt
     6001 gatagaacgc gtattcctgc gcggcgtcgc cttggtgccg ccggaagaaa tcctgccagg
     6061 cgttccggct gctcagccgg atgacgaaat ccttttccgt caacccaaag gagcggagcg
     6121 tgtcaatgag aagcgcgatg agctcggcat cggcggcagg gtcactctca ccgataatgt
     6181 cggcgttgaa ctggaagtgc tcgcgcaagc ggcctttttg ctggcgctca taccgaaaga
     6241 gctgtggcag ggcgaaccat ttgaacggtt tcttgtagcc ccgctcgtag gcggcaacca
     6301 tacgcgccag ggtgggagtc atctccgggc gcagcgaaac ctcacgctca cccttgtctt
     6361 tgaaattgta aagctgcgcg acgatttccg cgccgctctt ggtggtgaag agttccagcg
     6421 gttcgagagg gggaccgtca tactcgcgaa aaccatagcg ccgggccatg ccgcgccact
     6481 tgtcgaaaat gtattggcgc gcgtccgcgc tccacaagtc aggctttggc agcggctcag
     6541 gatagaaatc gcgaaacccg ggaagacgtt ccataattcg aggcagaagt taagcggtgc
     6601 ccgtgaaaaa gcaaaagcaa aacggcgtgc cagccaaaca ctggcagcgc gcgggaaatg
     6661 gccaacggct ccggctcagg ccttgggcgg cttggtgggc ggcttggcgg cgggtttggg
     6721 cgtaagtttg cggaccgaga ggcgcatctc tctgccagct ttgaatttaa ctacggaacg
     6781 ctcaggaatc ggcacctcgg ttcccggcgc actcggattg cggccagtcc gcgccttgcg
     6841 caccctgacg ctaaatatcc caaagttgcg cagttccact ttttcccctt gggccagggc
     6901 cttgataatg tgatccaacg tccgctggac gatgtcgaac acttgctgct gggccaagtc
     6961 ggtatcctcg cagatgcgaa gcaccaggcc gcgctttgtc agagtcatag gcaatgaatt
     7021 atgttgttaa gtcctatcaa ttgcgttggt tatagcttcc gccgccgcag aggtcaagcc
     7081 ggaaagttaa gcaagttttc cagggttcta cgcttcccaa gtgcccgccc caaactcgtt
     7141 cccccccaca ctctcccctg cctggcagat tcacttgcgg gtctgcacgc tgccgattag
     7201 ctcgcgcaag tccttgatgc ggttctgctg cataaactga cggaggccgg caatgacttg
     7261 cagagcagtc tggggctcgt aaaagttagc tgtgccgacg gcaacagcgg ttgcgccggc
     7321 cataaggaac tcgatggcgt cagcggtgga ttgaatgccc cccatgccga tgatggggat
     7381 tctcacagcc ttcgccgcct cccagaccag cttgatcgcg atgggcttga tgcacgggcc
     7441 agtcaggccg cccgtgacat tggcgagcct gggccggcgc gtctcgatgt cgatcgccat
     7501 ggccgggaag ctgttcatga tggacaacgc gtcgctgccg gctgcttccg ctgctttcgc
     7561 gtagggcgca atgctgacca cgttggggct cagcttggtt atcaagggga gtttcgtcac
     7621 cttccggcac gcgcctacca cctggctgag caatgtcaca tctgttccga actgtgcgcc
     7681 gccttccttg atgttggggc aggagacgtt caactccagg gcgccgacgc ctccgagagc
     7741 gtcaaagcgc cgcgccacct cggcgtattc ctcaaccgtc gtgccccaga tgttaatgat
     7801 cgtgggcacc ttcagcgctt tgaggaacgg ccagtacttc cggatgaaac cgtccacacc
     7861 cggtccctgg aggccgatgg cattaatgag gccgctggtg acctcaaccg tccgcggggt
     7921 cgggttgccg cggaccggtc cgagccggat gcctttgacg acgaccgcgc cgagctggtt
     7981 gagattgatg agctgtgagt attcgacgcc gtaaccgaat gtgccggagg cgacggtaac
     8041 cgggttctgc agagttaggg tgccgatctt gacagataag ttcatttggc tgcctcccag
     8101 gcaacttgtc gggaatcgaa aaccggccct tcggtgcagg tgcgccggta ttcccaacta
     8161 tcgccggctt tgatcgggac cacgcaggcc aggcacacgc cgatgccgca gcccatgtgc
     8221 tcatcgagtg agagttcagc ctgcagatcg aactcggctg cgaggcggcc aaccgccttc
     8281 aacattgggc tggggccgca ggcgaacagc ttgcggcccg gtgtgcgccg cttcagctcg
     8341 gtcaatagcg cttcagttaa gaggcccttt tcgccgtagc tcccgtcctc agtcgtgacg
     8401 cgcacctccc agccaagcgc gcgaaattcc tcctcgcaca gaatgtccac acgccgccgt
     8461 ccgccaacga acacaatacc cttttgcggc gagcgttgcg ccaacaggta gagcgcggcc
     8521 atgccatagc cgccggcgac caggagcggg gtctcgccag acggcggcgg cacggtgaac
     8581 ccgtggccga gcgggccgat catgctcagt tcctcgcccg ggcgcatgcg cgagagcacc
     8641 tcggtaccct tgccgatggt cttgtagagg atggacagcg tgctgccgga aacctggaag
     8701 atgctgaatg gccgccggag cagcgcctcc ctcaacggca gaatacggac gtgagcgaac
     8761 tgccccggct gcaccagcgg cgcaatccca ggcgcgcgga ccaccagccg aaaataaaga
     8821 tcagcctcgc gctcgttgga gacaatctgg accgtttgtt cgaccatcaa cgtcgagcat
     8881 gggcagggct ttggagcgtg tcaatgttgc gatgtgcctg gacgccctga aatccgaagc
     8941 ccgaaccccg agacccgaac aaggcttgcg ccagcacgcg tttgtcctgc tggagcggag
     9001 tgtattcggc cttcgggctt cggatttctt tcggccctcg gtcttcgggt ttcgaattta
     9061 gaccgcaccc gaccggcttc agggcaccgt tgctccgata gcctctccgt cgcgcttggc
     9121 gacggccctg gtgagcaggt cacggcaagt cgccatcgcc agcagcggcc aagtattccg
     9181 atacatatcg tacttgaggt agtaaacctt cgggaagccc gtgcccgtgg tctcgtgctc
     9241 ggtccaggag ccgtccgcgt tctgcatgcg aatcaaatac tcgataccac gccggacgct
     9301 ggggcggttc ggatcatcga acgtgcagag gcccatgacc gcccaagccg tttgcgacgc
     9361 ggtgctcggt ccctgaccct tgaaaacggg gtcgtcatag gtgttgcagc gctcacccca
     9421 gccgccgtcc tcgtgctgca cgctttccag ccaatcgcgg gctttggtca gccacggctc
     9481 gttcatgtcg aggttgaggg cgcgcagacc gcgcaacacc tgccaggtgc cgtaaacgta
     9541 attaacgccc cagcggccgt accacgagcc atcctcctcc tggtgctcgc ggatgaagcg
     9601 cagcgccttc tgcacctgcg gatgctcgac gctccagccc tcgtaaccca gcagttccaa
     9661 aatgcgggcc gtgatgtcgg cgcactcggg gtcgagcatc gcgttatggt cggcgaatgg
     9721 caccttctcc agcacgcctt tggtgcagtc tttatcgaac gcggcccagc cgccgtctct
     9781 gcattggaag gtcatcatcc atttcaagcc gcgctggaag cattcgtcgc gcttctgccg
     9841 gtcatccgtc gggaccttgc gcagcgccag aagcaccatg gccgtgtcgt ccacgtccga
     9901 gttccatttg ttctcgaatt cgaacaccca gccgctgggc tcgaccttag cggggttctt
     9961 gtattgccaa tcgccccgga agcggatctc cctggccatg agccactcgg tggcgcgctt
    10021 cagcgccgga tgattggccg gcacgccaga ttcactcagg cacatgttga cgatggcggt
    10081 gtcccagact ggcgagaggc agggctcgat gtgcacgctc tgttcgcttt catgctcgag
    10141 cttcttcaac tctcgctccg cccgcaccac cagcgggtga tcgtccggat acccgagcgc
    10201 cttgagggcg atgagcgaat tgagcatggc cggaaagatg gccgccaagc catctgagcc
    10261 ttcaaaccgc tccagcatcc attgctcggc cttgcgtagc gctcgcttgc gaaacggatg
    10321 aatgtgatgc tgagcgaacc attccgccaa cttgtgcacc cggtcgagcc agaggaagaa
    10381 attgcgccag gtgatccgct ccgggtcggg agccagcgcc aggtcgcgtt cgtggatgcc
    10441 ttccgggtaa agctcgtcaa tatcaatgtt cttcttcagc agccgggtcg gcctgtagtg
    10501 attgatgatc gccagcggca cgagcatgga ccggctccag gaactcatct cgttgaagtt
    10561 cacgtagaac catttgccga tgaggatcac ctcacaggga atggtcggca catactccca
    10621 ggagtacaag cccagcagcg ccagatacag cttcgagaac gtattcatgc gcggcacgcc
    10681 cccgaggttt aaggccacct cgcgtgcgcg cagcatgcgg gggtcggtca ccggcacgcc
    10741 ggccagcttc agcgccaggt agcatttgat ggtcgcgttg acctccgccg ggccgccgta
    10801 atagatgttc cagcccccgt cggacaactg catcgagaag atgtgattca ccgccttgcg
    10861 gtgccattgc gggtccactt tgccgttcca atggtggtag gcaatcgtgt ccgccaccag
    10921 cgtcgcatcc accatcagct cgccgaccca gtagccttcg ggcttctgct ggctgagcaa
    10981 atagctctgc gaccgctcga tggcggcttc caactcaatc tgaggcttta acccgaggtc
    11041 ctgttgacgc gggtttccct ctatgcggcc ttctcgcaaa gatactgcat ccacactcat
    11101 actctgtgga atcaagcccg aagtgtaaag cgttaaccgc tcccgcgcga atggtggtgc
    11161 gggccacggt cgccttaccc gccgccttta gccgcctgga tcgcccgggc cacctgctcg
    11221 atcgcctcca ggtgccgcta acggtacttc tccactgtcg gcttccaggg cgtctggagg
    11281 aaacccagca gatgccccgc gccgaggctt cggcgacact cttcagcgag cagctcgaaa
    11341 ttcgtcgagt tatagtagtt gctcccggtc ggaatctggt cgtagccgtg ctggtccagt
    11401 tcccggaacg ccttggccac agccttctgc gggtcgaact tcgcgtcgta ataccagtta
    11461 ctctggacca ccgacttcgg catcttccgg taaaacagct ccgggtggtg ccagacatag
    11521 tccgaccaga tccaggcgcg cgcgccgctc ttctcgacct gcgcgaccag gagcaggaag
    11581 tcgcgccacc acgcgtcata ctggcggatg acaacgtgct cgtagtactt ctggtttccg
    11641 taatcctcct cgtccattcc caggtggaaa aaccggggcc ggtcgaagag ttgcatcact
    11701 tcagcaatca gatcgcggca gacggcataa tacgtgtcgg tcgagacgca ccgcgcgtat
    11761 ttgcctaacc aggcgtcatg tgtggtcgag aaattcagct tgggaattgg ctccaggccc
    11821 agcgcccgca tccgcttcaa ttcaccgcgc aattcggcgg tgctccaggc gcccttaatc
    11881 gcgatctccg ggtggctctt gtacttcacc ccgtcgccca ggtcgttcac caccatgttc
    11941 atcccggcct gctgcatctt cttgaggagg tcgttccaca agctccgatc gaaccggagc
    12001 tcaggccggt aaatggagcc gtcgccctta agctccgggg catcccagtc gcaccacata
    12061 ttcgtgctca ggtgcagcag gttgccccaa attatcggat gtttgccgct ccgtgcaacc
    12121 tccgtcgagg ccgttggctc ggctccgccg acccgggcgc cgcccaccag ggttccaacc
    12181 gatgccgcgg ctgctagctt cagaaaacct ctgcgatcta tgatcatacc cgcgagcgta
    12241 tccacgcccg cagcctggtc aaccccgaag tggcaccccg aagggcatcc gagacaacac
    12301 cagccgagtg aaaggctatc cttttgaaac accgttgccg gccggccagc ccatcagcgg
    12361 cgtggtgctg gccgaccacg cccggtcatt ggattggaat tcgcgccacg ccgagttgat
    12421 ctgccagctt cccgccgaag tggttcagga cattgccgcc aaaatcatcg cgctggtcgc
    12481 ggaggattga aaactgaacg gatcgagtgg aacggaggcg tttctgatga ccgttggcgc
    12541 cgcgtcaaac caaacgaaac ccggtgctgg atcacttct
//
LOCUS       DATCGS010000231         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_714116, whole genome shotgun sequence.
ACCESSION   DATCGS010000231 DATCGS010000000
VERSION     DATCGS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714116"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VIL39_04725"
     CDS             <1..598
                     /locus_tag="VIL39_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HEY5480762.1"
                     /translation="LPGYFGGQRWHYYRLRAEGQNTLVINPGQGPDQDPAAKTQITRF
                     ESTPKRAFAIADLTPAYAKNASRVWRGIALLDRKNVVVQDEIQADKPIELWWFMHTPA
                     SVRIEDDGRTATLQLAGAQLRAEILSPASAKFQIMDAQPLPASPHPERQAKNERIRKL
                     AIHLTGISDTRLVVRLVPKPSEGEETGQAPKLSALSEW"
     gene            604..>3111
                     /locus_tag="VIL39_04730"
     CDS             604..>3111
                     /locus_tag="VIL39_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480763.1"
                     /translation="MRHLSSALLLAATLATATGTPAMRVVRTGSTGTPLLVNGSFEEL
                     PQGKPTPWFAWQQGFRVAPGEGRGGSQCLVCERSEKDGESGASQSLTLNRTNIAPFII
                     RGWSKAENVSGSPDNGYSLYVDIVYADGTPLWGQTANFTCGTHDWEQRQFVLLPDKPV
                     KSLTLHCLFRGHTGKVWFDDASVEEVPTPAGAVLFQGVPLQRVTHEKPDTKAAPRKLS
                     TQDGLKLTLRDNTITSLQLEGKELSAPSPSGFLVRDFAADSDVYAFAGGVCQELSLNL
                     QASFTAAKDHLAIEGRVTDTTGRDRAVTLLFALPLDATGWQWGDDVRRSRLIDGAGEF
                     ANTISLRCGANGKMSLYPLAAVWNERAGLALALDMDHPAQYRLVYHAGTRQFFIAYDF
                     GLTKDTAKNPSSAGFRFVLYRFDPRWGFRAALQKYYDLFPQHFVKRVTREGVWMPFTD
                     IAKVPGFDDFGFAFQEGAPNVAFDDQHGVASFVYVEPMSHWLAMPRDVPRTYESALSV
                     LDKDLAGTRGNEAAEMAAATLTSGIENADGRLLLHLVKAPWCDGGVFSLNPDPDVPTT
                     PNHPFNKAMVMHKAIAATFKKNEPKPGPPVSQPSTLNSQPLPGPGLDGVYLDSLEMSS
                     GELNYRREHFRTASVPLVFDREGRPCQLMIFNTWTFERDIAAQMHARGKLLFANAVLW
                     QFAFPAPLLDVLGTEVNWLRHDEYQPDSDAVMNFRRALCRQKPYCLLMNTDYGKFTPE
                     LVERYFQRCLFYGVWPGFFDQEAASKDPYWASAKRWHERDRPLFKKYIPLLRRVTAAG
                     WQPLTYGMCDNLAILVERFGPGTDGTVFLTLLNDTAHT"
BASE COUNT          596 a         1041 c          910 g          564 t
ORIGIN      
        1 cctgccgggc tatttcggcg gccaacggtg gcactactat cgcctccgcg ccgaaggcca
       61 gaatacactg gtcatcaatc cgggccaggg ccccgaccag gacccggcgg ccaagacaca
      121 gataactcgc tttgaatcca cgccgaagcg ggcgtttgcc attgccgatc tcacccctgc
      181 ctacgcaaag aacgcgagcc gcgtctggcg cggaattgct ctcctggacc gcaagaacgt
      241 tgtggtacag gacgagattc aggcggacaa accgatcgag ctgtggtggt tcatgcatac
      301 cccggccagc gtgagaattg aggacgacgg ccgcacagca accctccaac tagccggcgc
      361 gcagcttcgt gcggagattc tttcgcccgc gagcgcgaag tttcaaatca tggacgccca
      421 gccgttgcca gcttccccgc accctgaacg gcaggcaaag aacgaacgca tccggaagct
      481 ggcgattcac ctgaccggca tcagcgatac ccggctcgtg gttcggctgg tgccaaagcc
      541 ctccgagggt gaagaaactg gtcaggcacc gaaactgtcc gccttgtccg agtggtaatg
      601 ggcatgagac acctatcctc cgctctcctg ctcgccgcca ctttggccac cgcaaccggc
      661 acgccggcca tgcgcgtcgt gaggaccggc agcacaggga ctccgctgct ggtcaatggc
      721 agctttgaag agctgccgca aggcaagccg accccttggt ttgcctggca gcaaggcttc
      781 cgggtcgcac ccggcgaagg acgcggcggt tcacagtgct tggtgtgtga gcgcagtgaa
      841 aaggacggcg aatcaggtgc cagtcagtcg ctcacactca accggacgaa cattgcgccg
      901 tttattatcc ggggttggag caaggcggag aatgtgagcg gcagccccga caacggctac
      961 tcgctctacg tggacatcgt ctatgcggat ggcactcccc tgtggggcca gaccgcgaac
     1021 ttcacgtgcg gcacgcacga ttgggaacag cgccagttcg tgctgctgcc ggacaagccg
     1081 gtcaagtcgc ttacgttgca ctgcctcttt cgcgggcaca cgggcaaggt gtggtttgat
     1141 gacgcctccg tggaagaagt cccaaccccg gcgggcgcgg tgttgttcca aggcgtcccg
     1201 ctgcaaaggg tcacccatga gaaacccgac acaaaagccg ccccgcgcaa gctctccacg
     1261 caggacggcc tgaagctgac gctgcgcgac aacaccatca cgtcgctgca gcttgaggga
     1321 aaggagttga gcgctccgtc gccttccggc ttcctggtcc gggatttcgc ggcggactca
     1381 gacgtctatg cgttcgccgg cggcgtctgc caggaactta gtttgaacct gcaagcgagt
     1441 ttcactgccg ccaaggatca tcttgccatc gaaggccgcg tgaccgatac cacgggccgg
     1501 gaccgcgcgg tgacgctcct tttcgccctg ccgctcgacg cgactggttg gcaatggggt
     1561 gacgatgtgc gccgaagccg cctcatcgac ggcgccggtg agtttgccaa caccatcagt
     1621 ctccgctgcg gcgccaatgg gaagatgtcg ctttatcccc tggccgcggt ctggaacgag
     1681 cgcgccggtc tggcgctggc gttggacatg gatcatccgg cccaataccg cctcgtctat
     1741 catgccggca ccaggcagtt cttcatcgcc tatgacttcg ggctaacgaa ggacacagcg
     1801 aagaatccca gcagcgccgg cttccgcttc gtcctgtacc gcttcgaccc gcgctggggc
     1861 tttcgggccg ccttgcagaa gtattacgac ctcttcccgc agcactttgt caaacgcgtg
     1921 acccgcgaag gcgtgtggat gccgttcacg gacattgcca aagtgcccgg cttcgacgat
     1981 ttcggtttcg ccttccagga gggcgcgccg aacgtcgcat tcgatgacca gcatggcgtc
     2041 gccagcttcg tctatgtcga acccatgagt cactggctgg ctatgccccg cgacgtgccg
     2101 cgcacttacg agagtgcgct gtccgtcttg gacaaggacc tcgccggcac ccggggcaac
     2161 gaggcggcgg agatggcagc agccactctc accagcggaa tcgagaacgc agacggccgc
     2221 ctcttgcttc acctggtgaa agccccctgg tgcgatggcg gcgtgttctc tctcaatccc
     2281 gatccggacg ttcccacaac ccctaatcat cccttcaaca aggcgatggt catgcacaaa
     2341 gccatcgccg ctaccttcaa gaagaacgaa cccaaaccgg ggccgccggt ctctcaaccc
     2401 tcaaccctca actctcaacc acttcccggc cccggcctcg acggcgtgta tctcgactcc
     2461 ctcgaaatgt catccggcga gctgaactac cgccgcgagc actttcgcac cgccagcgtc
     2521 ccgctcgtct tcgatcgcga gggccggccc tgtcaactga tgatcttcaa cacgtggacg
     2581 ttcgagcgcg acatcgccgc acaaatgcac gcgcgcggca agctcctgtt cgcgaacgcc
     2641 gtgctctggc agtttgcctt ccccgccccg ctgctcgacg tgctcggcac cgaggtcaac
     2701 tggctgcgcc acgacgaata ccaacctgac tccgacgcag tcatgaactt ccgccgcgcc
     2761 ctctgccgcc aaaagcctta ttgcctgctg atgaacacgg actatgggaa gttcacgccg
     2821 gagctggtag agcgctattt ccagcgctgc ctcttctacg gcgtctggcc cggcttcttc
     2881 gaccaggaag ccgcgtccaa agacccgtat tgggcctcag cgaaaaggtg gcatgagcga
     2941 gaccgcccgc tgttcaagaa gtacatcccg ctgctgcggc gcgtcaccgc ggcaggctgg
     3001 cagccgttga cttacggcat gtgtgacaat ttggcgatcc tggtcgagcg tttcggccct
     3061 gggaccgacg gcaccgtgtt cctgacactg ctcaatgaca cagcgcacac g
//
LOCUS       DATCGS010000232         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_70769, whole genome shotgun sequence.
ACCESSION   DATCGS010000232 DATCGS010000000
VERSION     DATCGS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70769"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1583
                     /locus_tag="VIL39_04735"
     CDS             396..1583
                     /locus_tag="VIL39_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480764.1"
                     /translation="MTKSRMAGQARGPGLTGEERPRPAVVVGGQATHIALPSDLCAFS
                     RHFLSDSTRNPQLPTGLRVALVDGDERVHDFVRQAFKAHANGWVLDSHLTSHPALGAF
                     GPIPATPRSLRSKAPTSPQTLHTHAPRGTGGRRSYMATPSSTVYPPPFVPSRLCCHLR
                     RCRPGFTLIELLVVIAVISILAALLLPALGAAKERAVSAQCQNNLRQLGLAEHMYVDQ
                     NGGALPYAWATGNDPNRNNFEALLAPLVQKAAFGAGSSNGNSDFVRNVFRCPVRMREN
                     LWRQYPTYNGHGNPWKISYGMNQFNSVDYHAGDPGNVSPSGRTYALAFVPHPAQTLSI
                     ADLSYLKNHPPLADLGHQDIGYKHGGRLQIGDGAAEMLFLDSHVEGRKFGRTGDVILD
                     FRQ"
     gene            complement(1646..1789)
                     /locus_tag="VIL39_04740"
     CDS             complement(1646..1789)
                     /locus_tag="VIL39_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480765.1"
                     /translation="MILRVALEALIETPLMRRTPVQTDLDERLPNQARAPEKQQGGAC
                     SDA"
     gene            1805..2413
                     /locus_tag="VIL39_04745"
     CDS             1805..2413
                     /locus_tag="VIL39_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEY5480766.1"
                     /translation="MGQLNARGKSESWDGDIFPYVKRLAGVSDPYRQFQCPSDAVPRP
                     QPRSYAMTFYDPVRYGWPPRPDSHGGLGLYVDADRLRVLEAHAPEDPARSSARSMRLP
                     ALRFDMVPDPKGTGLLVESISADHFFGQAGGAAVECSRDQFNTNGPNSKTSANLHGGR
                     LNYLMADGHVETLHPWESVGWAGDAHGGIGDHFGLWSLRPDD"
     gene            2445..>4010
                     /locus_tag="VIL39_04750"
     CDS             2445..>4010
                     /locus_tag="VIL39_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5480767.1"
                     /translation="MKWFEGSIVLRAIRRLIGRANQSWRALAGRTPNWAMGLGMLLTV
                     AGFLVWRLTGLQPQKAQAVGEISEARLFQKNCAQDPLANRYAGIHSKASGFGVFIYDA
                     DTGQERAVGEVPQTKFAWSYLNFAGWSPDGSLFALTLDGKHIDWCDGRTGRKLGQSLS
                     RSALHTLAWLGNGWVTGWDDNHALQVIHSQPGAAGSDFVLPPLTGAAEEPRSLSVWQT
                     NTVLWLASNGVWELTVDTGARKKIWSDPTNRPIALSVSAETGQAVLCCRGPRTDLLYA
                     LDLNSPSPMPARLLAEDKGISDVAAIGDTNGFAYCKPGGGRRMFYVKANATAGMNALE
                     LGNDSCFSGCRGRYLYFKAQLTNEPPSLWQYDMLAERWNNVFPPWPKPLGQARLLFSC
                     RRAVVNARGHAGGYTMLPAANYTRGKKYPVLMGVMNYDWASFGEAIYAQTTANAGAYF
                     VAIDQRWRVDMKAEAIEENADDAYAIYQQLIQNPNVDKDRIFVFAMSAGGLYLRKLVA
                     EHPGLWRGLIAYST"
BASE COUNT          768 a         1205 c         1240 g          797 t
ORIGIN      
        1 tgtaccaagt gtttgcggcg ctgccgtcgg tcccgcgctc gcctccggtg tccggcaatc
       61 ctgactatgg gtaaagacta ggcaaagtga gggggagcag taggtttatc tatgtttgac
      121 cccggaaagt ggattgcggg gcgcgtgcag atcggcacaa ccaaaggggc gaaaaccttg
      181 ctccatcatt tggcccatga accaccccag cataaatccg ccaggatgaa caaagccgat
      241 ggacagcttg aattccaatc tacggacccc tttccttatc accggcctgc tcgaaggcgt
      301 gagttgcgcc cgttccagat tcttgttcca gatttgtgtt gacatttccg cttatgttgt
      361 gtttgatgcc ggaagtcagg agaagcggga ttcttatgac gaaaagcaga atggccggcc
      421 aagcccgcgg tccggggctg acaggagagg aacgccctcg ccccgccgtc gtagtcggcg
      481 ggcaggccac ccacatcgcc ctgccaagtg acctgtgcgc cttctcgcgg cacttcctca
      541 gcgactcaac ccgcaaccct caactcccaa ccgggctccg cgtcgccctc gtggacggcg
      601 acgaaagggt ccacgatttc gtccgccaag cgtttaaggc tcacgccaac ggctgggtgc
      661 tggatagcca cctgacttcg catccagcgc tcggtgcttt cggccctata cccgctactc
      721 cgcgctcctt gcggagcaag gccccgacca gtccgcaaac tctccatacc catgccccgc
      781 gagggaccgg cggacgcaga agttacatgg ctactccatc ctccaccgtc tatcctccac
      841 ccttcgttcc ttcgcgtctt tgttgtcatc ttcgccggtg ccgtcccggc tttacgctca
      901 tcgagttgtt ggtggtcatt gccgtcatca gcattctggc ggccctgctg ctgccggcct
      961 tgggcgcggc gaaggagcgg gcggtttccg cccaatgcca gaacaacctc cggcagttgg
     1021 ggctggccga gcatatgtat gtggaccaaa acggaggggc gctgccgtat gcctgggcga
     1081 cggggaacga ccccaaccgg aataattttg aggcgttgct ggctccgctg gtgcaaaagg
     1141 cggcgtttgg cgcgggcagc tccaacggga acagtgattt cgtgaggaac gtgtttcgtt
     1201 gtccggtgcg gatgcgggaa aacctctggc ggcagtaccc gacttacaac ggacacggca
     1261 atccgtggaa gatcagctac gggatgaacc agttcaattc ggtggactat catgctggcg
     1321 accccggaaa cgtctcgccg agcggcagaa cgtatgcgtt ggcatttgtg ccgcatccgg
     1381 cccagacctt gtccattgcc gacctgtctt atctaaagaa ccatcccccc ctggctgacc
     1441 tcggacatca agacatcgga tacaagcacg gcggtcgttt acagattggg gatggggcgg
     1501 cggaaatgct gtttctggac agccatgtgg aaggccgcaa gttcggcagg accggggacg
     1561 tgattctgga ttttcgccaa tgacctttag gcgcaagaaa acgcggtgcg gacgacatcc
     1621 accggctctt gcgcgcattg acgtcttatg catcggagca ggcgccgccc tgttgcttct
     1681 cgggggcgcg ggcctggttc ggcagtcgtt catccaggtc cgtctgcacg ggtgtgcggc
     1741 gcatcaaagg agtctctatc agggcttcca aggctacgcg caggatcatt tcaacgggct
     1801 gcccgtggga caactgaatg cccgtgggaa aagcgaatca tgggacggcg acattttccc
     1861 ctacgtgaag cggctggccg gggtatcgga tccctatcgt cagtttcagt gcccgtcaga
     1921 tgcggtgccg cggccgcagc cgcgcagtta cgcgatgacg ttctacgacc ccgtgcgtta
     1981 cggctggccg ccccggccgg acagtcatgg cggccttggg ctgtatgtgg atgccgaccg
     2041 tttacgcgtg cttgaggcgc atgcgccgga agatcccgcg cggtcatcgg cgcggtcaat
     2101 gcggctgccg gcgctgaggt ttgacatggt tcccgacccc aaaggcaccg gattgctggt
     2161 ggaaagcatt tccgccgatc atttctttgg ccaggccggg ggagcggcgg tggaatgcag
     2221 ccgcgaccag ttcaacacca acgggccaaa ttcaaagacc tccgccaatc tccatggcgg
     2281 aaggctcaat tacctcatgg ccgacggcca tgtggagaca ctgcatccgt gggaaagcgt
     2341 cggctgggcg ggtgatgccc atggcggtat cggcgaccat ttcggcttat ggtcgctgcg
     2401 gcctgacgat tagagtccaa agaagcgggc aagggcgcaa aagtatgaaa tggtttgaag
     2461 gcagcattgt gttaagggcc atacgccggt tgattggcag ggcaaaccag tcatggcggg
     2521 ccttggcggg ccgaacgccc aactgggcta tgggcctggg gatgttattg actgtggccg
     2581 gctttctggt ctggcggctg acgggcctgc agcctcaaaa agcgcaagcc gtgggggaaa
     2641 tcagcgaggc caggcttttt cagaagaact gcgcgcagga cccgctggcc aaccgttacg
     2701 ctgggattca cagcaaggcc agtggctttg gcgtgttcat ctacgatgcc gacacgggac
     2761 aggaacgtgc cgtgggcgaa gtcccgcaaa cgaagtttgc ctggtcctat ctgaacttcg
     2821 ccggctggtc gcccgacgga agcctttttg ccctcacgct ggatggcaag cacattgact
     2881 ggtgcgacgg gcgcacgggc cggaaattgg ggcaaagcct ctcgcgcagc gccttgcata
     2941 cgctggcctg gctcggcaac ggctgggtga ccggctggga cgataaccac gcgcttcaag
     3001 tcatccattc ccaacccggg gcggccgggt ctgacttcgt cttgccgccc ttgaccgggg
     3061 cggccgaaga gcctcgcagt ctttccgtct ggcagacgaa caccgtcctc tggctggcga
     3121 gcaatggtgt ctgggagttg acggtggaca ctggagcgcg caagaagatc tggagcgatc
     3181 ccaccaaccg ccccatcgcc ctgtccgtct ccgccgaaac cggccaggcc gtcctctgct
     3241 gtcgcgggcc gcgcaccgac ctcctctacg ccctggatct caattccccc tcgcccatgc
     3301 cagcgcggct gttggcggag gacaagggaa tttccgacgt tgccgccatc ggggacacaa
     3361 atggctttgc ctactgcaag cctggtggcg gtcggcgcat gttctatgtc aaagccaatg
     3421 cgacggctgg aatgaatgcg ctggaactcg gaaatgattc ctgcttttcg gggtgccgcg
     3481 gcagatacct ctacttcaag gcgcagttga ccaacgagcc gccctccctc tggcagtatg
     3541 acatgctggc tgagcgatgg aataatgttt ttccaccatg gccgaaacca ttggggcagg
     3601 cgcggctgct cttcagttgc cggcgggcgg tggtcaatgc ccgtggtcat gcggggggat
     3661 acaccatgct gccggcggcc aactataccc gcggcaagaa ataccccgtg ctgatgggcg
     3721 tgatgaatta tgactgggcc tcctttggcg aggccatcta tgcacagacc accgccaacg
     3781 ccggagccta cttcgttgcc atagaccagc gctggcgcgt ggatatgaaa gccgaagcca
     3841 tcgaggaaaa tgcggatgac gcctatgcca tctaccagca actcatccag aaccccaacg
     3901 tggacaaaga ccgcatcttt gtttttgcga tgagcgccgg cggcctctac ctcaggaaac
     3961 tggtggcgga acaccccggg ttatggcgcg gtttgattgc ctacagcacc
//
LOCUS       DATCGS010000233         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_216355, whole genome shotgun sequence.
ACCESSION   DATCGS010000233 DATCGS010000000
VERSION     DATCGS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216355"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1011
                     /locus_tag="VIL39_04755"
     CDS             337..1011
                     /locus_tag="VIL39_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HEY5480768.1"
                     /translation="MHFLRKKFEGSPDYARVAPFVIFVVLTAMQGQFGEDSRYWLYLV
                     KTLVGVWLIWEMRPFVEEMRWKISWEAVVVGVAVFAIWVGLDGLYPRLSELDDAGWNP
                     NKQFGQGSALAWLYVVVRIAGSSIVVPPLEEAFYRSYVYRVLVKTDFRSMPLGQFHLL
                     SFVVTSSIFGLMHPDRWLGGILCGLAFQWLVIRKNRLGDAMTAHAVTNFLLGLWVVWK
                     GAWNFW"
     gene            1027..>2317
                     /locus_tag="VIL39_04760"
     CDS             1027..>2317
                     /locus_tag="VIL39_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5480769.1"
                     /translation="MLPCLIFEDDHLLVVNKPAGLNTHAPSPYAGEGLYDWLRHRETR
                     WAKLAIVQRLDKETSGVMVFSKSPLANRSLTRQFTDRVVRKKYLLLTDRPVHPREMKV
                     ESTLVRAGNRYVSRPLHPSGETAQTHFRVLGEADLEGGNPKAEARRPKEIRNPKPETR
                     RGSSEGGWCELVEAEPLTGRTHQIRVHAAEKGFPILGDTLYGGTPAPRVYLHAAELTL
                     RHPASGQEMTFRAPFEQGTHLAGAAPQRRDSHEPAPADLAEPGDGLRWLTGPLPCLSL
                     REALIEPEQTNAYRVIHGASDGWPAWYLERLGDFLLSQSEHALHPAQREELARLAKTF
                     AARGVYHKILTRQVRRAAPAEASPQLVLGEAAPERFTVRENGVQFELSFAEGYSVGLF
                     LDQRDNRRRLLTGHVAAGFSLLPSSLQSPIADGKWQIL"
BASE COUNT          419 a          701 c          751 g          446 t
ORIGIN      
        1 gctcaagatc gagcagcccg gcgaccagac cctcaaagtc cgtccgaaag acgccgcaag
       61 ctggaaggcc atcaacctcc gcttcatcaa actgaccccg gccatctgaa tgtcgccggc
      121 gcgcggcatt cctgtccgca gcaagctccg aaggtcaacg cccttccggc caattcgcta
      181 gccatcttgc ccattgaaca ttgctgcgga caagaatgtc cgcgctccgc tccttcggcc
      241 gtgacggcgg cggcagtgtc cgaatgcgcc cacccctgtt tgcgcggcca cttttggctt
      301 ggcgcgggtg ggggatagcg ggatgattgc ccgccaatgc acttcctgcg caagaaattt
      361 gaaggttcgc ccgactacgc gcgagttgcg cccttcgtga tcttcgtcgt cctgactgcc
      421 atgcaaggcc agttcggcga agactcgcgc tattggcttt acctggtgaa gacgctcgtg
      481 ggcgtgtggc tgatctggga gatgcgcccg ttcgtcgagg aaatgcgctg gaagatcagt
      541 tgggaggcgg tggtggtggg cgtagccgtc tttgccattt gggtgggcct ggatggactg
      601 tatccgcggc tcagcgaact ggatgatgcg gggtggaatc ccaacaaaca gttcggccaa
      661 ggctcagccc tcgcgtggct gtacgtggtg gtgcgcatcg cgggctcatc catcgtcgtg
      721 ccgccgttgg aggaagcttt ctaccgctcg tacgtctatc gggtgctcgt aaagactgat
      781 ttccgctcga tgccgctcgg ccagttccat ttgctctcgt tcgtggtcac gtcgtcaatc
      841 ttcgggctca tgcacccgga ccgttggctg gggggcatcc tgtgcggtct ggccttccaa
      901 tggctggtga ttcgcaagaa ccgtctgggg gacgcgatga cggcgcacgc ggtcaccaat
      961 ttcctgctcg ggctttgggt ggtgtggaag ggcgcctgga atttctggta aagggatcat
     1021 cgcccggtgc tgccgtgcct catttttgaa gacgaccatc tgctggtggt gaacaaaccc
     1081 gccggcctga acacgcatgc gcccagcccg tatgcgggcg agggcctcta cgactggctg
     1141 cggcatcggg agacgcgctg ggcgaagctg gcgattgtcc agcggctcga caaggagact
     1201 tccggcgtca tggtcttctc taaatcaccc ctcgctaatc gctcgcttac caggcagttc
     1261 accgatcgcg tcgtcagaaa gaaatacctc ttgctcaccg atcgccccgt gcacccgaga
     1321 gagatgaagg tcgagagcac tctggttcgc gctggtaaca ggtacgtgag ccggccgttg
     1381 catcccagcg gagagaccgc ccagacgcat ttccgagtcc tgggagaggc ggacttggag
     1441 gggggaaatc cgaaggccga agcccgaagg ccgaaggaaa tccgaaaccc gaaacccgaa
     1501 acccgaaggg gatcgagcga gggcggttgg tgtgagctgg tcgaggctga gccgttaacg
     1561 gggcggaccc accagatccg agttcacgcg gcggagaagg ggtttccgat tctcggcgac
     1621 acgttgtatg gcggcacacc ggcgccgcgg gtttacctgc acgcggctga gctgacgctg
     1681 aggcatccgg cttcgggcca ggagatgacc ttccgcgcgc cgttcgagca agggacacat
     1741 cttgcgggtg ccgcaccaca gcgaagagac tcccacgagc cagcgcctgc ggacctcgcc
     1801 gaacccggcg atggtctgcg ttggctgacg ggtcccctgc cctgcctgtc gttgcgcgaa
     1861 gcgctgatcg aaccggagca gaccaatgcc tatcgggtca tccacggcgc gagtgatgga
     1921 tggccggctt ggtatctcga gcggctggga gacttcttgc tatcgcagag cgagcacgct
     1981 ttgcacccgg cgcagcgcga ggaactggcg cggctggcaa agaccttcgc cgcgcgcggg
     2041 gtgtaccata agatcctgac acgtcaagtg cgtcgcgccg ctcccgccga agcgtcacca
     2101 caactggtgc tgggagaggc ggctccggaa cggttcacgg ttcgagagaa cggcgtgcag
     2161 ttcgagctca gttttgccga gggctattca gtgggcctgt tcctggacca gcgagacaat
     2221 cggcggaggc tccttacagg acatgtcgcc gctggcttct ccctgctccc gagctccctg
     2281 caatcgccaa tcgcagatgg caaatggcaa atcctca
//
LOCUS       DATCGS010000234         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_417288, whole genome shotgun sequence.
ACCESSION   DATCGS010000234 DATCGS010000000
VERSION     DATCGS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417288"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIL39_04765"
     CDS             complement(<1..252)
                     /locus_tag="VIL39_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480770.1"
                     /translation="MRPFVLLAVMLALAAGWWCPSAAGAGTLVEPAASTNAAVPGTEL
                     AHTISEITGVAISPLLGVSAVGAWKYFHARTPQQAARLPW"
     gene            complement(428..2602)
                     /locus_tag="VIL39_04770"
     CDS             complement(428..2602)
                     /locus_tag="VIL39_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY5480771.1"
                     /translation="MIAAALLTSLGSTIRAAEPAEKPLYLDPAQPAEKRAQDLIARLT
                     LEEKAMLLNHKGTTVERFGIKCDRWNQCLHGVCWDRPTTLFPVPIALAATWDSNLVHE
                     VATAISDEARAIYNGWHQDPAFPGEHKGLIYRAPVINIERNPYWGRNEEAYGEDPYLT
                     GRLGVAYVKGLQGNDPHYLKLVSTLKHYAVNNVEQGRTSLSAAVPERMLYEYWLPHFR
                     DCIVEGQAQSVMASYNAINGVPSNINPLLLTEILKQRWGFQGFVVSDLGGVNTMVTGH
                     MKGHMTYEEAVAKSLIAGCDFSDKEFEAHLPAAVRQGLLPEARLNDAVFRVLRDRIRL
                     GEFDPQELVPYSKIPTNVICSPAHRALALKAVRESIVLLVNRNNFLPLNKSKLKTIAV
                     IGPHADTFTSGGYSGKAENPVKPLQGIRNRAGQGTEVLYARGCAISGGANEGDSIAQA
                     AAVARGADVAIVYVGTNNKIESEGRDRTSLSLPGRQEDLVKAVCAANPNTVVVLMNAG
                     PLTVPWLKENVPAILEAWWNGVEGGDAIADVLFGDFNPAGRLPHTIYASESQVPPQDE
                     YDVTKGFTYMYLNGTPLFPFGHGLSYTRFKYSNLRVSPKQIPATGKVTVRVDVENTGK
                     RAGDEVVQLYVHDVKCSVKRPSKELRGFERVALRPGQKKTVAFTLAGEKLSFWDVKTH
                     GFVVEPGAFDVLVGSSSEDIRVHTRIEVNLKTAVEQQGTKGT"
BASE COUNT          536 a          891 c          897 g          570 t
ORIGIN      
        1 ccagggcaga cgggcagctt gctgcggagt ccgagcgtga aagtacttcc acgcgcccac
       61 agcgctgacc cccagcagcg gggagatcgc gacgcctgtg atctctgaga ttgtatgagc
      121 cagctccgtg cccggcactg cagcgttggt ggacgctgcc ggctcaacga gggttcctgc
      181 cccggcggcg gacgggcacc accaaccggc ggccaaggcc aacatgacgg ctaagagaac
      241 gaacggtctc ataccacaca ggctccgcct tttaggcatg tctgtaaagc ggggccttcg
      301 ctcgctctgc gggcggttct tgcatcctga agggattgta tcatgcagcc cagggggccg
      361 ttcgctcacc caatcctatt gaagggattg tataagctcg cccagggttg gcccgtcgtg
      421 gtgcaaacta tgtgcctttg gtgccttgtt gttcaaccgc ggtttttagg ttcacctcga
      481 tgcgggtgtg aacgcggatg tcctccgagg agcttccaac caacacatcg aacgcgcccg
      541 gctccacaac gaagccgtgg gttttcacat cccaaaatga gagcttctcc ccggcaaggg
      601 tgaacgcgac ggtcttcttc tggcctggac gcaaggcgac ccgctcgaat ccgcgcagtt
      661 ccttgctggg ccgcttcaca ctgcatttca cgtcgtgaac atagagctgg accacctcgt
      721 cgcccgcgcg cttgcccgtg ttctccacat ccacgcgcac ggtgaccttg ccggtcgctg
      781 gaatctgctt gggtgagacc ctcaaattcg agtacttgaa cctggtgtag ctcagcccgt
      841 gaccgaaggg gaagagcggc gtgccgttca gatacatgta ggtgaaaccc ttggtgacat
      901 cgtattcatc ctggggcggg acctggcttt cggaagcata aatggtgtgc ggaaggcgcc
      961 ccgccgggtt aaagtcgcca aagagcacgt cagcaatcgc gtcgccgccc tcgacgccat
     1021 tccaccatgc ctccaggata gccggcacgt tttctttgag ccagggaacg gtcaacgggc
     1081 cagcgttcat caggaccacc acggtgtttg ggttggccgc gcaaaccgcc ttgaccaggt
     1141 cttcctgacg gccgggcagt gacaggctcg tgcggtcacg gccctcggac tcgatcttat
     1201 tgttggtgcc gacatacaca atcgccacgt ccgcaccccg ggcgaccgca gcggcctggg
     1261 cgatggaatc accttcgttg gcgccgccgc tgatggcgca tccgcgcgcg tagagcacct
     1321 cggtcccctg ccccgcgcgg ttgcggatgc cttggagggg cttgacggga ttctccgcct
     1381 tgccgctgta gccgccggag gtaaaagtgt cggcatgggg gccgatgacg gcgatcgtct
     1441 tgagtttgct cttgttcagc ggcagaaagt tgttccggtt gaccagcagc acgatggatt
     1501 cgcggaccgc cttcaatgcc agcgcccggt gcgccggact gcagatcaca ttcgtgggga
     1561 tcttgctgta aggcaccagc tcctgggggt caaactcgcc caggcggatg cgatcacgca
     1621 ggacgcggaa cacagcatca ttgaggcgcg cctcgggcag caacccctgc cggaccgcgg
     1681 cgggcaggtg ggcctcgaac tccttgtcgg agaagtcgca accggcgatg agggatttgg
     1741 ccacggcctc ctcataggtc atgtggcctt tcatgtgccc agtcaccatg gtgttcacgc
     1801 cgcccaggtc cgacaccaca aagccttgaa atccccatcg ctgcttgagg atttcggtga
     1861 gcaagagcgg gttgatgttg ctgggcacgc cgttgatcgc attgtaggag gccatgaccg
     1921 actgggcctg gccttcgacg atgcaatcgc ggaagtgcgg cagccaatat tcgtaaagca
     1981 tccgttcggg gacagcggcc gagagactgg tgcggccctg ctcgacgttg ttgacggcgt
     2041 aatgtttgag cgtggaaacc agcttcaagt aatgcgggtc attgccctgc aagcctttga
     2101 catacgccac gccgaggcgg cccgtcagat agggatcctc tccataagcc tcctcattgc
     2161 gcccccagta agggttccgt tcgatgttaa tgacgggtgc gcgatagatc agccccttgt
     2221 gctcgccggg aaaagccggg tcttgatgcc agccgttgta gatggcgcgc gcttcgtcgg
     2281 agatggcggt ggcaacctca tggaccaggt tggaatccca cgtcgccgcc agcgcaatcg
     2341 gcactgggaa cagggtggtc ggacgatccc agcacacccc atggaggcac tgattccacc
     2401 gatcgcactt gatgccaaag cgctccaccg tggttccctt atgattaagc agcatcgcct
     2461 tctcctccag cgtcaggcgt gcgatcaaat cctgggcgcg tttctcggcg ggttgcgccg
     2521 ggtccaggta aaggggtttc tcagcgggct cagccgcccg aatcgtgctt cccagcgacg
     2581 tgagcagcgc cgccgcgatc actagtgcgc ttgcagcgtt gctgcctcca ccaacgaatg
     2641 aatacatatc gagacccttc taactcgcaa gcctgcggag agcaagtcgc aaacagagct
     2701 gcaacgcaat tccagtatca gccccgaatg ctctcgggcg caatggatgt tcgcgcggcg
     2761 agcatggtgg tggtccgcag gatgggagaa gccaagcccc tgccgtcgca aacgtttaag
     2821 ccggcagagt tccgacggct tcctgtcgcg gttgccgctg cgcgggtggg gcgctgcttg
     2881 acactgccac cggg
//
LOCUS       DATCGS010000235         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_120659, whole genome shotgun sequence.
ACCESSION   DATCGS010000235 DATCGS010000000
VERSION     DATCGS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120659"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VIL39_04775"
     CDS             complement(<1..457)
                     /locus_tag="VIL39_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480772.1"
                     /translation="MPYFPTRKTTIFAQDPSIRLDGKIVRAQIEIPNEALQPGPRGYR
                     VQVVDYDSSTHTLYKTMPHPSNRADGLPDDPFCEMTDDALLASPDFHAMNTYAIIMRT
                     LAHFEFALGRRVAWSFPGHQIQVAPHAFADANAFYSPEDQALMFGYFPRH"
     gene            complement(726..1982)
                     /locus_tag="VIL39_04780"
     CDS             complement(726..1982)
                     /locus_tag="VIL39_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480773.1"
                     /translation="MASFASETGGPGAATGRFPVRVWLIAFLGWMFDFYDLILFSFLL
                     IPIGKDLALSETEESLLLGVALGASGVGGILFGYLSDRYGRKRVMTWTIGLYSLGTAL
                     CAFSTGPMSLLVFRLLTGLGVGGEWAVGHALLQEASPSHMRGRAAAFLQAGEPIGVGL
                     AAVMGLLVSPFLGWRWVFLVSSASAVLAFVARRHLPESPLWGRQEEERLSPLAALVWL
                     KRHHYLGPLWKGWLLGVFKLGTYWTCYIWLPKFLQNQLHQSIGNSAKWILTAQSGQLL
                     GMLLFGWAADRYGRRQAYTVYSLLTASALYLLAFHWQQLLLNTTLFWAVMFAMGLGSG
                     CTAGFGALLAELFPTRIRNFAMGTAYNCARGVQFFAPMVVSAFVALDGVRGGLSVPLV
                     LALATAAWVWVLPETRHRDLARITAH"
     gene            2026..2670
                     /locus_tag="VIL39_04785"
     CDS             2026..2670
                     /locus_tag="VIL39_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480774.1"
                     /translation="MKPERCYFFPPMASLEKDAEIELARDLVAGNLGAFDRFVEAFHS
                     RLFQYSMLVCGHPEDAEEVAQDTLLNVFKNFARLREPEHVRAWVFRIARNACLMKRRK
                     SVFAPAHELSLDELMPAAGDRTGERKLEIADWSALPDAQVLRAELKDTLMEAIQSLPD
                     LYKTVLLLRDVEELSTEETAGILDVSHDVVKTRLHRARLAVRQKLDGYLQARAN"
     gene            2670..2954
                     /locus_tag="VIL39_04790"
     CDS             2670..2954
                     /locus_tag="VIL39_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5480775.1"
                     /translation="MLHKSPLPPGECADLFARLSEYLDGELSPEARRAMEQHICDCPP
                     CIEFIHSLRRTIDLCHHLEPAGSPAPVSREAKQELLSVLQRALAARDPGR"
     gene            3001..>3264
                     /locus_tag="VIL39_04795"
     CDS             3001..>3264
                     /locus_tag="VIL39_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter YccM"
                     /protein_id="HEY5480776.1"
                     /translation="MAKKLVKRRAPDHSQLLRWSVQCAFLLLNIWIGVQFYFFVRHYE
                     TFGATALVDRPPGVEGWLPIASLMNLKVLLLTGTLPAVHPAGVF"
BASE COUNT          662 a         1040 c         1014 g          548 t
ORIGIN      
        1 cgtgcctggg aaagtacccg aacatcaacg cctggtcctc cggcgaataa aacgcgttgg
       61 catcggcgaa cgcgtgtggc gcgacctgaa tctggtggcc cggaaaactc cacgccacgc
      121 gacggccgag tgcgaactcg aaatgcgcga gcgtccgcat gatgatcgcg tacgtgttca
      181 tcgcgtggaa atcggggctg gcgaggagcg cgtcgtcggt catctcacag aatgggtcgt
      241 cgggcaagcc gtcggcccgg tttgacggat gcggcatggt cttgtacagc gtatgcgtgg
      301 aggagtcata gtccaccacc tgcacgcggt atccgcgcgg gcccggctgc agtgcttcgt
      361 tggggatttc gatttgtgcg cgcacgatct tgccgtcgag ccgaatgctc ggatcctggg
      421 cgaatatcgt ggtcttgcgg gttggaaagt aaggcaagtt ccctctctta gcatgaataa
      481 tacgcgcttt cttaccgaat ttccagagga cattccgcgg cagatgagtc acgtggccaa
      541 aaggcgaatg ttgacggcgc aaaactattg gaagaaccgg ggcccggcga aatctatctc
      601 cggaggcgtg gggcagaccg tcgtcgtttg tggtctgccg tttgcgggcc gccaggcata
      661 gcagacgaca caaaacgatc gtctgctcca cgcaacataa ccggcattct gcttgccgca
      721 ctgcactagt gcgccgtgat ccgagccaag tcgcggtggc gtgtctccgg cagcacccac
      781 acccaggcgg cggttgccag cgccagcacc agcggcaccg acaagccgcc gcgcacgccg
      841 tcgagggcca caaaggcgct gacaaccatg ggcgcgaaga actggactcc gcgcgcgcaa
      901 ttgtaggccg tacccatggc gaagttgcgg atgcgagtcg ggaacaactc cgccagcagc
      961 gcgccgaagc ccgccgtgca acccgagccc agccccatgg caaacatcac ggcccagaag
     1021 agggtcgtgt tcagcaacag ttgctgccaa tggaaggcca acaggtagag ggcggaggcc
     1081 gtgagcagcg agtacacggt gtaggcctgc cggcggccgt aacggtctgc cgcccatccg
     1141 aacagcagca tgcctaagag ctgtcccgat tgcgccgtca atatccactt cgccgagttg
     1201 ccaatggact gatgcaactg gttctgcaga aacttgggca gccagatgta gcaggtccag
     1261 taggtgccca gcttgaagac acccagcagc cagcccttcc acaacggccc gaggtaatga
     1321 tgccgcttca gccaaacgag agcagccagc ggggaaagtc gttcttcctc ttgccggccc
     1381 cacagcggag attcgggcaa gtgccgccgg gctacaaagg ccaggaccgc cgaggcggag
     1441 gaaaccagga aaacccagcg ccagcccagg aatggcgaga ccagcaggcc catcacggcc
     1501 gccagaccca ctccgatcgg ttcgccggcc tgaaggaaag cggcggcgcg cccgcgcatg
     1561 tgcgagggag aagcttcctg caagagagcg tggcccactg cccattcgcc gcccaccccg
     1621 agcccggtca acagccggaa gaccagcagg ctcatcgggc cggtggagaa agcgcacagc
     1681 gccgtgccga gcgagtacag gccgatggtc caggtcatca cgcgcttgcg cccgtagcga
     1741 tccgacagat agccaaacag aattccgcca acgccggaag cgcccagcgc tactcccagc
     1801 aagagcgatt cttcggtctc gctgagcgca agatccttcc caatcgggat cagtaagaag
     1861 gaaaacagga tcagatcgta gaagtcgaac atccagccca ggaaggcgat cagccacacg
     1921 cgtaccggga agcgcccggt agcggcgccg gggccgccgg tctcactcgc gaaagaggcc
     1981 atgaccggaa catgatatca cgaagcgtgt cacgcgtccg ctacaatgaa gccggagcgc
     2041 tgttacttct tcccaccgat ggcatctctt gaaaaggacg cagaaattga gctggcgcgc
     2101 gacctggtag ccggcaatct gggggccttc gatcgatttg tcgaggcctt tcactcccgg
     2161 ctgtttcagt acagcatgct cgtctgcgga catccggagg acgccgagga agtggctcag
     2221 gatactctgc tcaacgtgtt caagaacttc gctcggcttc gtgagcccga gcacgttcgc
     2281 gcctgggtct ttcgtatcgc ccgcaacgcc tgcctgatga agcggcgtaa gagcgtgttc
     2341 gccccggcgc atgagctatc cctcgatgaa ctgatgcccg ccgccggcga ccggaccggc
     2401 gagcgcaagc tcgagattgc ggattggtcc gcgctgccgg atgcacaggt gctgcgcgcc
     2461 gagctcaaag acaccttgat ggaagcgatc cagagcctgc cggatctgta caagaccgtt
     2521 ctgcttctgc gcgacgtaga ggagctttcg accgaggaaa ccgccggcat tctcgacgtc
     2581 agccatgacg tggtgaagac gcgcctgcac cgcgcccgcc tggccgtccg gcagaagctg
     2641 gacggctatc tgcaagcgag ggcgaattaa tgttacacaa gtctccactg ccgcccggcg
     2701 agtgcgcgga cctctttgcg cggctctccg agtacctgga cggtgaactc tcgccggaag
     2761 cccgccgggc catggagcag catatttgcg actgcccgcc ctgcatcgag ttcattcaca
     2821 gcctgcggcg cacaattgac ctttgtcacc acctcgagcc cgcgggttca ccggctccgg
     2881 tcagccgcga agcgaagcag gaactgctgt cagtgctgca gcgcgcactg gccgcccgcg
     2941 accccggccg gtgaccgcgg tcactgctcc gcgcccccca ctccggtacc ctgaaaggga
     3001 atggcgaaga agctcgttaa acgccgcgcg cccgatcatt cgcagcttct gcgttggagc
     3061 gtgcagtgcg ctttcctgct gttgaacatc tggatcggcg tccagttcta cttcttcgtc
     3121 cggcattatg aaacgttcgg cgccacggcg ctggtcgacc gtccgccggg cgtcgaaggc
     3181 tggctgccga tcgcgtctct catgaacctg aaggtgctgc tgctcaccgg aaccctcccg
     3241 gccgtgcatc ccgcgggcgt gttt
//
LOCUS       DATCGS010000236         5578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_516589, whole genome shotgun sequence.
ACCESSION   DATCGS010000236 DATCGS010000000
VERSION     DATCGS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516589"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2307)
                     /locus_tag="VIL39_04800"
     CDS             complement(<1..2307)
                     /locus_tag="VIL39_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5480777.1"
                     /translation="MQPFPSPLAELPALNQVAIPDLWQQQAVSALREGKDVVVQAPTG
                     AGKTLIFELWSNQGKNRGQAIYTVPTRALANDKLAEWRGRGWDVGIATGDLAENLGAP
                     VLVATLETQKNRLIQGDGPTLLVVDEYQMLSDLDRGLNYELALALAPQQTQLLLLSGS
                     VGNPQDVVKWLLRLGRKAVLVRHDERPVPLDEVHANSLSYHVPSEIRGYWPRLVAKAL
                     AEDLGPILIFAPRRQAAESMANELSRQLPTPNPLQLSPEQKLVVGEHLAKLLKSRVTY
                     HHSGLSYGARAGVIEPLAKAGQLRVVVATMGLAAGINFSLRSVALAGESYRRNEAEQL
                     LKPDELLQMFGRAGRRGLDETGFVLITANELRLLDSHTGHLSRSAAVDWSALLGIMTA
                     AAQHGRDPFREAVRTQERLFTTKPIFLGVEESLRHPDVPCGLRTDAERARHVHKRVRE
                     MEDSRGEWEPYPTPIPVPVKDVRVSPIVNHQSPNAPTPAGPANWQSAIGDWPSVLSLP
                     AALEKVGTGTLCVLSEDEHGKIHGRALTVADRLGHDRVLLAKWIRRLTNWNGRQVPLS
                     VWEEKIVPLVKERLAQQKTPVVSFQNQQQRILALVSIADLTMRVPVDRHGVALWKPVE
                     REVLPADCAQCPLVPACRQLPTSTGVALLWRRLGLVNAAGVPTLRGRVVSFFSQGDGL
                     AIAAALEDKTYPLDELIYDLADLDAGFRFAGEDINRWGGRLGIVCHKTYGLQSIPGYL
                     ENGVPPKYGAGAEQIVASVHKNPLAKQVW"
     gene            2481..3545
                     /locus_tag="VIL39_04805"
     CDS             2481..3545
                     /locus_tag="VIL39_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5480778.1"
                     /translation="MFAADSKVVCAPVTAAPNGETACTWKQIIDPVEPFLEAVGQRLA
                     KQVEAFDPDIVRYADYALNGQGKHLRAALVALTAKALGKVNDAHITVAVIIETVHLAT
                     LVHDDVIDEAEIRRGQPTLAANWSNEIAVLFGDCLFAHALVLAASFPTPDVCRAVARA
                     TNTVCTGEILQIQRRRDFTFTREKYFKVLEMKTAELFALSCELSAGISGMDAECRLAM
                     RRFGLAFGTAYQVYDDCVDLFGSEAVAGKSLGTDLAKGKLTLPVLLLWERADAADRAR
                     LRELVEGWQSGSMSCLEELLAKYDALAASLEIIHQYLEEARQTLLGLPASKGRAGLLG
                     LAEYLARQADGLGAVREGSL"
     gene            3542..4192
                     /locus_tag="VIL39_04810"
     CDS             3542..4192
                     /locus_tag="VIL39_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480779.1"
                     /translation="MIMTEPARRKAPQPADEPEKASPAEPVPTEEMELVGRARRGDLS
                     AYDELVRRYQQRIYATVYHMTANHEDANDLAQEAFIKAFQALRSFKGGSSFYTWIYRI
                     AVNKTINFLKQRRNKAQMSLDDLDFNAEHDPDLVALISDKTPRRDVALAELQEKLNAA
                     MQRLSEPHRLVVTLHDVQGLSHEEAAKIMGCNIGTVRSRLFYARQQLQAYLSDYLK"
     gene            4195..4710
                     /locus_tag="VIL39_04815"
     CDS             4195..4710
                     /locus_tag="VIL39_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480780.1"
                     /translation="MSEDTENFEQLRRLLVLKRYEQPPPRYFNDFSAQVIRRIKAGEK
                     GERGDIFERLFDEAPWLQRIWAAFETKPILAGVLGMAVCGFLIGGMFYSDTTEVPPFA
                     LMPGVETAPGAQASMAVADHPLLIKPVVLQQPSSTSPITAVQTDAFLLGDLAKLRSGT
                     IPASFSFPGGN"
     gene            complement(4720..>5578)
                     /locus_tag="VIL39_04820"
     CDS             complement(4720..>5578)
                     /locus_tag="VIL39_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480781.1"
                     /translation="YLILLSSVLYPLSYWTVQMATGDEDPRAMRILSRLGQDLQSTGF
                     AKVARAVDRMPQVWYDSADLAGLIYKNPLSEARLARYPAFLGLAERPEFQDMANDTQF
                     TELLMRPAPIMTVLDHPKAQAVIQNPALVQLIWSTVVPDLKDVTTFLVTAKSAKYDSE
                     KILGRWNFNVSVTMSLFRRAKPNMSSKEMTQWKKWMMSAFAKTSLVAMTDHQAILKNA
                     PQLKLPAAGATASGGPQTLKGEWKDLDTKYQLALSGGSKEELLTATIEGDRLTISADG
                     LGLAFDRED"
BASE COUNT         1007 a         1583 c         1885 g         1103 t
ORIGIN      
        1 ccagacctgt ttggcgagcg ggttcttgtg gacggaggcg acgatctgct cggcacctgc
       61 gccgtatttg ggcggcacgc cgttttcgag gtagccgggg atggattgca ggccatacgt
      121 cttgtggcaa actatcccca gtcgcccgcc ccaacggttg atgtcctcgc cggcaaagcg
      181 gaacccggcg tccaggtccg cgaggtcgta gatcaattca tcgagtggat aggttttgtc
      241 ttcgagcgcg gcggcgatgg ccagcccatc tccctggctg aagaagctga cgacccggcc
      301 gcggagggtg ggcacgccag cggcattgac caggccgagc cgccgccaaa gcaaggcgac
      361 gcctgtgctg gtcggcagtt gacggcaggc ggggacaagc gggcattggg cgcagtcggc
      421 gggcaggact tcgcgctcga cgggcttcca gagggcgacg ccgtgccggt ccaccggcac
      481 gcgcatggtc aggtccgcaa tgctgacgag ggcgaggatg cgctgctgct ggttctggaa
      541 gctcacgacg ggcgttttct gctgggccag cctctctttt accagtggga caatcttctc
      601 ttcccagacc gacagcggca cctggcggcc gttccaattg gtcaggcgcc ggatccactt
      661 ggcgagcagg acgcggtcgt ggccgaggcg gtcggcgacc gtgagggcgc ggccatggat
      721 cttgccgtgc tcgtcttcgg acaggacgca aagggtgccc gtgccgacct tttcgagggc
      781 ggcggggagg gagaggacgg atggccaatc gccaatggcc gattgccaat tcgcaggtcc
      841 ggctggcgtc ggggcatttg gcgattggtg attgacgatt ggcgatacgc gtacgtcttt
      901 caccgggacg ggaattggcg ttgggtaagg ttcccactcg ccacggctgt cttccatttc
      961 gcggacgcgc ttgtggacgt ggcgggcgcg ctcggcgtcg gtccgaaggc cgcacgggac
     1021 atccgggtga cgcaaggatt cctcgacgcc gaggaagatg ggcttggtcg tgaagagccg
     1081 ttcctgggtg cgcacggctt cgcggaaggg gtcacggcca tgctgggcgg cggcggtcat
     1141 gatgccaagc agggcggacc agtccaccgc ggcgctgcgc gagaggtggc ccgtatggga
     1201 gtccagcagg cgcagctcgt tggcggtgat cagcacgaag ccggtttcgt ccaggccccg
     1261 acgcccggcg cggccgaaca tttgcagcag ctcgtcgggt ttgagcagtt gctccgcttc
     1321 gttgcgccgg taggattcac cggcgagggc gacgctgcgc agggagaagt tgatgccggc
     1381 ggccaagccc atggtggcga cgaccacccg aagctggccg gccttggcca gcggctcgat
     1441 gacgccggcc cgggcgccgt agctcaatcc gctgtgatga taggtgacgc ggctcttcaa
     1501 cagcttggcc aagtgctcgc ccacgaccag cttctgctcg gggctgagct ggaggggatt
     1561 gggtgtgggc agttgccggg agagttcgtt ggccatggat tccgccgcct ggcggcgcgg
     1621 ggcaaagatc aggatcggtc ccaggtcttc ggccagggcc ttggcgacga gccggggcca
     1681 gtagcctcgg atttcgctcg ggacgtggta gctcaggctg ttggcatgaa cttcgtcgag
     1741 cggcacggga cgttcatcat ggcggaccaa aacggccttg cggcccagcc gcagcagcca
     1801 tttgacgaca tcctgcgggt tgcccacgct gccgctcagc aggagcagtt gcgtttgttg
     1861 cggcgccagg gccagcgcga gctcgtaatt caggccgcgg tcgaggtcgc tgagcatctg
     1921 gtactcatcc acgaccagca gggtggggcc gtcaccctgg atcaggcggt tcttctgcgt
     1981 ctccaacgtc gcgaccagca cgggggcgcc caggttttcc gccaggtcgc ccgtggcgat
     2041 gccgacgtcc caaccgcgcc cgcgccattc cgcgagcttg tcgttggcca gggcgcgggt
     2101 gggcaccgtg tagatggcct gcccgcgatt cttgccctgg ttggaccaga gctcaaagat
     2161 cagcgtcttg cccgccccgg tcggcgcctg gaccaccacg tccttgcctt cgcgcagggc
     2221 gctgacggcc tgctgttgcc agaggtcagg gatcgcgacc tggttgaggg cgggcagttc
     2281 ggccagtggt gaggggaacg gttgcacggc aaacgtgagt caaatcaggg taaattgggt
     2341 aattggggcg taggtcgtca gttgtggatg gcacgttatc atggggagaa gatacccaag
     2401 tttggcgaat ccgaaatggg aaagacgcgg cactatatgg ggtttgactt ggacacttca
     2461 cgacattaga cttcgcgcac atgtttgcgg ctgattccaa agtggtttgc gctccggtta
     2521 ctgcagcgcc gaatggcgag acggcttgta cctggaagca gatcattgat ccggtggagc
     2581 cgttcctcga agccgtcggg cagcggctgg cgaaacaagt cgaggccttc gacccggaca
     2641 tcgtccgtta cgctgattat gcgttgaacg ggcagggcaa gcatttgcgg gctgcgctgg
     2701 tggctttgac ggccaaagcg ttgggaaagg tcaatgacgc gcatatcacg gtggcggtga
     2761 tcatcgaaac ggtgcacctg gcgacgctgg tgcatgacga cgtgattgat gaagccgaga
     2821 ttcggcgggg ccagccgacg ctggctgcca attggagcaa cgagatagcc gtattgttcg
     2881 gcgattgcct gttcgcccat gcgctggtgc tggcggcgag ctttcccacc ccggacgttt
     2941 gccgggcggt ggcgagggcg accaatacgg tgtgcacggg ggagatcctg caaatccagc
     3001 gccggcgcga cttcaccttc acgcgtgaga agtacttcaa ggtgctggaa atgaagaccg
     3061 ctgagctgtt tgcgctttcg tgcgaactga gcgcgggtat aagtgggatg gacgccgagt
     3121 gccggctggc gatgcgccgg ttcggactgg cctttggcac cgcctatcag gtgtatgatg
     3181 attgcgtgga tctgttcggc tcggaagcag tggcgggcaa gtccctgggc acggatttgg
     3241 ccaaaggcaa actgacgttg ccggtgctgc tgctgtggga gcgggcggat gcggcggacc
     3301 gggcgcgctt gcgcgagctg gtcgagggat ggcagagcgg ttcgatgagc tgcctggaag
     3361 agctgctggc aaaatatgac gcgctggcgg cctctttgga gattattcac cagtatttgg
     3421 aagaggcgcg ccagacgctg ctgggtttgc ctgcctccaa gggccgcgcc gggttgctgg
     3481 gcttggccga atatctggct cgccaggctg atgggttagg ggctgtccgc gagggcagcc
     3541 tatgattatg actgagccgg cgcgccgcaa ggcaccccaa ccagccgatg aacctgaaaa
     3601 ggcctcaccg gcggagccgg ttccgaccga ggaaatggag ctggtgggcc gggcgcggag
     3661 gggggacctg tcggcttacg acgaactggt gcgccgctac cagcaacgca tctacgcgac
     3721 ggtgtaccac atgacggcca accacgagga cgccaacgac ctggcgcagg aggcattcat
     3781 caaggcgttt caagcgctca ggtcctttaa gggcggctcc agcttctata cctggatcta
     3841 ccggattgct gtcaataaga ccatcaattt cctcaagcag cggcggaaca aggcgcagat
     3901 gagccttgac gacttggact tcaatgccga gcatgatccg gacctggtgg cgctgatttc
     3961 ggataaaacc ccgcgccgcg acgtcgcctt ggctgaattg caggagaaat tgaacgcggc
     4021 gatgcagaga ctgtcagaac ctcatagact ggtagtgaca ttacacgatg tgcaagggct
     4081 ctcgcatgaa gaagcggcaa agattatggg atgcaatatc ggcaccgtgc ggtcgcgttt
     4141 attttatgcc cgtcagcagt tgcaggccta tttgtcagac tacttgaaat gagtatgagt
     4201 gaggataccg agaacttcga acagttgcgc cggttgctgg tgctcaaacg ctacgagcaa
     4261 cccccgcctc gttatttcaa tgatttctcc gcccaggtca tccggcgcat taaggccggc
     4321 gaaaagggcg agcgcggcga catcttcgag cggctgtttg atgaagctcc gtggttgcaa
     4381 cggatttggg cggcttttga aaccaagccc atcctggctg gagttctcgg aatggcggtg
     4441 tgcggctttt tgattggcgg catgttctat tcggacacga ctgaagttcc cccattcgcc
     4501 cttatgccgg gagtggaaac ggcgccggga gcgcaagcga gtatggcggt ggcggatcac
     4561 ccgctgttga ttaagccggt tgtgctccag caaccttcca gcaccagccc gattactgcg
     4621 gtccaaacag atgccttcct tctgggcgac ctcgcaaagc ttcggtccgg cacaataccc
     4681 gccagcttta gttttcctgg cgggaactga cctgccgaat tagtcctcgc ggtcgaacgc
     4741 gagacccagt ccatcagccg agatcgtcag gcggtcgcct tcaatggtag cggtcaatag
     4801 ctcctctttg cttccgcccg aaagggcaag ctgatacttg gtatccaggt ccttccattc
     4861 acccttcaag gtttgtggtc cgccggatgc cgtggcgcca gccgcgggca gcttcagttg
     4921 cggcgcgttc ttgagaatgg cctggtgatc ggtcatcgcc accaggctgg tcttggcgaa
     4981 agccgacatc atccactttt tccactgcgt catttccttg gacgacatat tcggcttggc
     5041 ccggcggaac aggctcatgg tgacgctcac gttgaagttc caacgcccga gaatcttctc
     5101 tgagtcgtac tttgcggact tggcggtcac caggaaagtg gtcacgtctt tgaggtcggg
     5161 gaccaccgtg gaccagatca actgcaccag ggcaggattc tggataaccg cttgggcttt
     5221 gggatgatcg agcacggtca tgatcggcgc gggccgcatg agcagttcgg tgaactgcgt
     5281 gtcattggcc atatcctgga attccggccg ctcagccagt cccaggaaag ccgggtagcg
     5341 ggccagccgc gcttcgctca gggggttctt gtaaatcagg cccgccaggt cggctgagtc
     5401 gtaccatacc tgcggcatcc gatcaactgc tcgcgccacc ttcgcaaaac cggtgctttg
     5461 aaggtcctga cccagcctac tgagaatcct catcgccctc ggatcttcgt cacccgtcgc
     5521 catttgcacc gtccaatagc tgagcgggta gagcacggac gaaagcagga tgagataa
//
LOCUS       DATCGS010000237         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_366374, whole genome shotgun sequence.
ACCESSION   DATCGS010000237 DATCGS010000000
VERSION     DATCGS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366374"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VIL39_04825"
     CDS             <1..254
                     /locus_tag="VIL39_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY5480782.1"
                     /translation="TPAEIRAFARFGADAVGMSTVPETIVARQCGLKVAAVSCITNLA
                     AGRSGKALSHAEVLETAERVKKLAAQVLKNFAKLYAQNQ"
     gene            238..651
                     /locus_tag="VIL39_04830"
     CDS             238..651
                     /locus_tag="VIL39_04830"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY5480783.1"
                     /translation="MPKTSKRDLVRAAAKARQRAVAPYSKFKVGAALLTRSGETVTGA
                     NVESASYGLTCCAERVALFNALTAGKRKFVAVAVVARAPGGPMPCGACRQLLAEYAPH
                     AVVWVADSRALHAIKEFSVKELLPAAFIAVPEDSD"
     gene            697..2217
                     /locus_tag="VIL39_04835"
     CDS             697..2217
                     /locus_tag="VIL39_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5480784.1"
                     /translation="MKSDVAFALENAGWPALLVDEGGTLCGASPAAKSLFGAALADES
                     PRLAAVWAPENTSTADELLAQWECAAAPTLLLKFRVKDGRAVSYLTWICALTKDGRKY
                     YVLQLLRESTPAATELKPPSMDAGQALKQKLDCALQLARTVALDFNNALTSILGHTSL
                     VLSQMEPSHPWRSSLIEVEKSAAKAAEIANDLGTFSRQEKEARVQAAGNLNPLLQRSI
                     EFFQQNAGPAPAAWTLQLDRKLFTAKFDEAKMQQAFMKVIENALQSLRGDQGRITVQT
                     RNLELAEPTQDRNAQLAAGAYVCVEITDNGCGIEPDILPRVFEPFFTTKRDGSHRGLG
                     LAWVYGIVTNHGGGVAISSQPGAGTSVRLYLPAERKLVRDNGVTSADLNGTQTILMVD
                     DEDLLLTMGKTILSAYGYRVLTANGGQKALEILAQKDTTVDLVITDLVMPAMSGRELV
                     ERIRQLSPRMRILCTSGYVWPAGRETDGAYLQKPFTSQELLLKVKQALNAEPSAED"
     gene            2263..3144
                     /locus_tag="VIL39_04840"
     CDS             2263..3144
                     /locus_tag="VIL39_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5480785.1"
                     /translation="MQIESLKVFCDLAETESFTKAAQINEVTQSAVSQQISSLERLFK
                     ALLIERSKKKFRLTREGQVLYDYSKQVIATYDALHSKLQETKDIISGTIRVATIYSIG
                     LHDLPPYLKKFLKSYPTVNVHVEYRRSNQVYEDVLGNTVDLGLVAYPMRDSKLEIYPL
                     RKEPLVLICHPKHPLAKNKSIRLKATNGQKFISFEPDIPTRRALDKILKEHSVRVQHV
                     MEFDNIETVKRAVEIDAGIAIVPQGTVLQEVAKQTLAQVTIEDGEFYRPLAALYKKNK
                     VLSPAIKQFLAVLKDEK"
     gene            complement(3266..>4080)
                     /locus_tag="VIL39_04845"
     CDS             complement(3266..>4080)
                     /locus_tag="VIL39_04845"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY5480786.1"
                     /translation="ATENADVASDRMALYSSITRPVLNYRVSKTRWCVLRWPTPSMAQ
                     AAGMSTEAFEDLYFAVCTLDYHKMARAMGPLERRLKRANRVHLKGPGTDLAFSIKGMG
                     AKMCKGDRNIPDGEVFSCPVKNSINGRIQFNTPTLYSGTKFENVCLEFKDGKIVKAVS
                     NNTRRLNEILDTDPGARYTGEFSLGFNPYIMSPMCDILFDEKIAGSLHLTPGQAYDEC
                     DNGNRSAIHWDMVLIQRPEWGGGEIWLDGELIRKNGMFLPRDLKPLNPANLK"
BASE COUNT          833 a         1246 c         1264 g          737 t
ORIGIN      
        1 agaccccggc tgagatccgc gcgtttgccc gattcggcgc ggatgcggtc gggatgagca
       61 ccgtgccgga gacgattgtg gcgcgtcaat gcggcctgaa ggtggccgcg gtatcctgca
      121 ttaccaacct ggcggccggc cggagcggaa aggccctttc gcacgcggag gtgctggaaa
      181 cggctgagcg ggtgaagaaa ctggcagcgc aagtgctcaa gaactttgcg aaactctatg
      241 cccaaaacca gtaagcgcga cttagtgcgg gcggcagcca aagcgaggca acgcgctgtg
      301 gcgccgtatt ccaagtttaa ggtcggcgcg gcgctgctca ccaggtccgg tgaaaccgtc
      361 acgggcgcca acgtggagag cgccagctat gggctgacct gctgcgccga gcgcgtggcg
      421 ctgttcaatg cgctgaccgc cggcaagagg aagttcgtcg ccgtggccgt cgtcgcgcgc
      481 gccccgggcg ggccgatgcc ctgtggggcg tgccgccaac tgctcgccga atacgcgccc
      541 cacgccgtgg tatgggtggc ggacagccgc gcgttgcacg cgatcaagga attctccgtg
      601 aaggaattgc tgccggccgc gttcatcgca gtgccggaag attcggattg agaaaggact
      661 tcgaggcggt aagctgccag ttggaagatc ctgttgatga agtccgatgt agcttttgcg
      721 ttggagaatg cgggctggcc ggcgctcctg gtggacgagg gtggcaccct ctgcggcgcc
      781 agtccggccg ccaagagcct tttcggcgcg gccctcgccg acgagtctcc ccgcctggcg
      841 gcggtttggg cgcccgagaa caccagcacg gccgacgagc tattggccca gtgggaatgc
      901 gccgccgccc ctacgctgct gctcaaattc cgggtcaagg acggccgggc ggttagctat
      961 ctcacgtgga tctgtgcgct caccaaagat ggccggaagt attacgtgct tcagttgctg
     1021 cgggaaagca cgcccgccgc gacggaacta aagccgccga gcatggacgc cggccaggcg
     1081 cttaaacaga agctggactg cgctttgcaa ctggcgcgca ccgtggcgct cgatttcaac
     1141 aatgcgctga ccagtatcct gggtcatacg tcgctcgtct tgagccagat ggagcccagc
     1201 catccctggc gcagctcgct gatcgaagtc gagaaatcgg ccgccaaggc ggctgaaatc
     1261 gccaacgacc tgggcacctt cagccgccag gagaaagaag cccgcgtgca agccgccggc
     1321 aatctgaatc ctctcctcca gcgcagcata gaattctttc agcagaatgc cgggccggct
     1381 ccggcggcgt ggactttgca attggaccgc aagctcttca cggcgaagtt tgacgaagcc
     1441 aagatgcagc aggcgttcat gaaagtcatc gagaacgccc tccagtccct gcggggagac
     1501 caaggccgca ttaccgtgca aacgcgcaac ctcgagctgg cggagccgac ccaggaccgc
     1561 aacgcgcaat tggccgcagg ggcttacgtg tgcgtggaaa tcaccgacaa cggctgcggc
     1621 atcgagccgg atatcctgcc gcgagtgttt gagcccttct tcacgaccaa gcgcgacggc
     1681 agtcatcgcg ggctcggttt ggcctgggtc tatggcattg tcaccaacca cggcggcggc
     1741 gtcgccatct ccagccagcc cggcgccggc acctccgtaa ggctttacct gcccgcggaa
     1801 agaaagcttg tgcgggacaa cggggtgacg tcggctgacc tcaacggcac ccagacgatc
     1861 ctgatggtgg atgacgagga tttgctgctg acgatgggca aaacgattct ctcggcgtac
     1921 ggttaccggg ttctgaccgc caatggcggg cagaaggcgc tggaaattct tgcccagaag
     1981 gacaccacgg tggaccttgt catcaccgat ctggtgatgc cggccatgag cggccgggaa
     2041 ctggtcgagc gcatccgaca gctttcgccc aggatgcgca tcctctgcac cagcggctac
     2101 gtgtggccgg cgggccggga gaccgatggc gcctacctgc aaaagccgtt caccagccag
     2161 gaactgctgc tcaaagtcaa gcaggccctg aatgcggagc cctcagccga ggactaaaac
     2221 ggcagttgac ggctttcatt aaatgtcgta ttcactgttc ttatgcaaat cgagagtctg
     2281 aaggtctttt gcgacttggc tgaaaccgaa agcttcacca aagccgcgca aatcaacgaa
     2341 gtgacccaat cggcggtgag ccagcagatc agttcgttgg aacgcctctt caaagcgctg
     2401 ctcatcgagc ggagcaagaa gaagttccgt ctcacccggg agggtcaggt gctttacgat
     2461 tacagcaagc aggtgattgc cacttacgat gcgctgcaca gcaaactgca agaaaccaag
     2521 gacatcatct ccggaaccat ccgcgtcgcc accatttaca gcatcgggtt gcacgatttg
     2581 ccgccgtatc tgaagaagtt cctgaagagc tacccgaccg tcaacgtgca cgttgaatat
     2641 cgccgctcca accaggtgta tgaagatgtg ctgggcaaca ccgtggacct gggcctggtg
     2701 gcgtatccaa tgcgcgacag caaactggag atctacccgc tccgcaagga gccgctcgtg
     2761 ctgatctgcc acccgaaaca tccgctcgca aagaacaaga gtatccggct caaggccacc
     2821 aatgggcaga agttcatcag ctttgagccc gatatcccca cccgcagggc gctggacaag
     2881 attctcaagg aacactcggt ccgcgtgcag cacgtgatgg agttcgacaa catcgagacc
     2941 gtcaagcgcg ccgtcgagat tgacgcgggc attgccatcg tgccccaggg tactgtcctc
     3001 caggaagtcg cgaagcaaac cctggctcaa gtgaccatcg aggacgggga gttctaccgg
     3061 ccactggccg cgctctacaa gaaaaacaaa gtcctctcgc ccgccataaa acagttcctg
     3121 gcggtcttga aggacgagaa atagcggcaa ctgttcgggt agtggggcgg ggcgtctcgc
     3181 ctgccaggcg gcgtctcgcc ctcggcccca gcgacgcggg cgagacgccc cgtgcggcag
     3241 gcaagacgcc cgccccacta ctcggtcact tcaagttcgc gggattgagc ggcttcaaat
     3301 cgcgcggcag gaacatcccg ttcttgcgga tcagttcgcc gtcgagccag atttcaccac
     3361 cgccccactc gggccgctga atcagcacca tgtcccaatg gatcgccgag cgattgccgt
     3421 tatcgcattc atcataagcc tggcccggtg tcaggtgcag cgagccggcg atcttctcat
     3481 cgaacaggat gtcgcacatc ggactcatga tgtatgggtt gaagccgagc gagaattcgc
     3541 ccgtgtaacg agcgccggga tcggtgtcca gaatctcgtt caggcgccgc gtattgttcg
     3601 acacggcctt tacgatcttc ccgtctttga actccaggca cacgttctcg aacttcgtgc
     3661 ccgagtaaag cgtcggcgtg ttgaactgaa tgcgcccgtt gatcgagttc ttgaccgggc
     3721 aggaaaacac ctcgccatcg gggatgttcc ggtcgccttt gcacatcttc gcgcccatgc
     3781 ctttgatgct gaaggcgagg tccgtgccgg gacctttaag gtgaacccgg ttggctcgtt
     3841 tcagccggcg ctccaacggc cccatggcgc gcgccatctt gtggtagtcg agcgtgcaga
     3901 ccgcaaaata gaggtcctcg aatgcctctg tgctcatgcc ggcggcctgg gccatgctgg
     3961 gcgtcggcca gcgcaacaca caccagcggg tcttgctcac gcgataattg aggaccgggc
     4021 gagtgatgct ggaatagagc gccattcgat cgctggccac gtcggcgttc tcggtggcgt
//
LOCUS       DATCGS010000238         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_615481, whole genome shotgun sequence.
ACCESSION   DATCGS010000238 DATCGS010000000
VERSION     DATCGS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615481"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VIL39_04850"
     CDS             <1..1549
                     /locus_tag="VIL39_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5480787.1"
                     /translation="AWRNRDAIYVGTDFGPGSLTDSGYPRIVKEWKRNSPLSEAKVIR
                     EGKKEDMSVSAGVVHDHGRTYEFISRAVTFFTNEEYLRQGDQWTRIDKPDDALVSTFE
                     DQLLLRLRSDWKVNGSTYPAGSLLAADFKQYLQGDRHLETLFLPTARKSLAAISATKK
                     YLVINELDNVKNRLYTLQHKNGQWTRTGLDTPAFGSVGLIGIDADESDDYFMTVTDFL
                     TPSSLYLGSLPRAGREKLKALPALFKTGGLEITQHEAISKDGTKVPYFQVSRTHLVLN
                     GTNPTLLYGYGGFEIPMLPAYSAGVGSAWLERGGVYVLANIRGGGEFGPTWHEAARKK
                     NRQRAYDDFIAVAEDLAARKVTSAKHLGIQGGSNGGLLMGVMLTERPNLFKAVVCQVP
                     LLDMRRFNKLLAGASWMDEYGNPDKPEEWSYISKYSPYQNVRPSTKYPRVLFTTSTRD
                     DRVHPGHARKMVARMKEQGHDVLYYENIEGGHGGAANNKQAAYMSALAYTFLLKELAP
                     QGSEVKP"
     gene            complement(1539..2195)
                     /gene="aat"
                     /locus_tag="VIL39_04855"
     CDS             complement(1539..2195)
                     /gene="aat"
                     /locus_tag="VIL39_04855"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY5480788.1"
                     /translation="MPIAILDCRLRFPSPECADAEGLVAIGGDMSLPRLLLAYRSGIF
                     PWTVDPITWWSPEPRAIFELDRFHVSRSLARVIRKGVFRITMDSAFRRVMQGCAARAP
                     GRRSTWVTPEFIEAYTLLHEQGHAHSLECWQGPRLVGGIYGVTIGGFFAGESMFHRVT
                     DASKIALFHLIEHLRQQGFELFDIQMLTPITTQLGGVTVPRKEYLQRLAQAVEKPAKV
                     "
     gene            complement(2278..2742)
                     /locus_tag="VIL39_04860"
     CDS             complement(2278..2742)
                     /locus_tag="VIL39_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480789.1"
                     /translation="MKLIRTTKTRLVFHLGYWEAHLFLEVLSLYPCLPPAHQKLSQTG
                     RLPEPEANQQLLDEALAEHRSDNKKQLQALLADQRRFAPTEKGGRLSLPPAEVEWVLQ
                     VLNDIRVGSWVLLGSPDKTSVQLNEKTAPHYRAMEIAGYFEAQLLEALGGET"
     gene            complement(2739..3047)
                     /locus_tag="VIL39_04865"
     CDS             complement(2739..3047)
                     /locus_tag="VIL39_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEY5480790.1"
                     /translation="MAATATPEIQPGVVQTTEEKPRSGLEPGFLVICWNDPVNLMDYV
                     THAFQVVFGWPRKKAEFHMLQVHEQGKSLLTRESMEKAEHYVHQLQKYSLHATMERDE
                     "
     gene            3198..>3413
                     /locus_tag="VIL39_04870"
     CDS             3198..>3413
                     /locus_tag="VIL39_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480791.1"
                     /translation="MNTTSTLRAIIISVALVAILASTLPGMGAEQPGKIKVLRVTGDD
                     AQPAHDWREVSQALRETLISSGRFDVRI"
BASE COUNT          715 a         1010 c         1028 g          660 t
ORIGIN      
        1 agcctggcgg aaccgggatg cgatttacgt gggcacggac tttggtccgg gctcgttgac
       61 ggactcgggt tacccgcgga ttgtgaagga atggaagcga aactctcctc tatccgaagc
      121 caaggtgata cgcgagggca agaaagagga tatgagcgtc agtgcgggag tggttcatga
      181 tcacggccgc acctacgagt ttatcagccg cgccgtgacg ttcttcacca acgaagagta
      241 cctgcgccag ggtgaccagt ggacccggat tgataaacca gacgacgcac tggttagcac
      301 tttcgaagat cagttactgt tgcgcttgcg ctccgactgg aaggtgaacg ggagcaccta
      361 tcccgccggg tccttgctgg ctgccgattt caaacagtac ctgcaaggtg atcggcacct
      421 cgaaaccctg tttttgccga ccgcccgcaa aagcctggcc gcaatcagcg ccactaagaa
      481 atacctggtc atcaacgaac tggacaacgt gaagaatcgt ctttatacgc tgcaacacaa
      541 gaacggccaa tggacgcgaa ccgggctgga cacgccggct ttcggttccg tcggcctcat
      601 cggcatagat gcggatgaat ccgacgacta ctttatgacg gtcacggatt ttctcacgcc
      661 ctccagcctt tatttgggtt cgttgccgcg cgcggggaga gagaagctca aggctttgcc
      721 ggcgcttttc aagaccgggg gtttggagat cactcagcat gaggccatct cgaaggacgg
      781 aaccaaggtg ccctactttc aggttagccg gacccacctg gtgctgaatg ggaccaaccc
      841 gaccttgcta tatggatacg gcggcttcga gattcccatg ttgccggcct atagtgccgg
      901 cgtcggttcg gcctggctcg agcgcggggg cgtttatgtg ctggcgaata tccgcggcgg
      961 gggcgaattc ggcccaacct ggcatgaagc ggcccgcaaa aagaaccgcc agcgcgccta
     1021 cgacgatttc atcgccgtgg ccgaagacct ggcggctcgc aaggtgactt cagcgaaaca
     1081 ccttggcatc caaggtggct ctaatggggg cttgctgatg ggagtcatgc tgaccgaacg
     1141 accgaacctg tttaaggcag tggtctgcca ggtgcccctg ctggatatgc gtcggttcaa
     1201 caagctcctc gccggcgcaa gctggatgga cgagtatggc aaccccgaca aacccgaaga
     1261 atggagttac atcagcaaat actcgccata tcagaatgtg cgcccgagca cgaagtaccc
     1321 gcgagtgctt tttaccactt ccacgcgcga tgaccgggtc caccctgggc acgccaggaa
     1381 gatggtagcg cgcatgaaag agcaagggca cgacgtgctg tattacgaga acattgaagg
     1441 ggggcacggt ggagccgcca acaacaagca agccgcctat atgtccgcgc tggcttatac
     1501 ctttctcctg aaggaactgg ctccgcaggg gagcgaagtc aaaccttagc aggtttctcc
     1561 accgcctggg cgaggcgttg gaggtattct ttgcgcggga cggtgacgcc gcccagttgc
     1621 gtggtaatcg gggtgagcat ctggatgtcg aacaactcaa aaccttgctg ccgcaggtgt
     1681 tcaatcaagt ggaacagagc gatcttggag gcatcggtga cgcggtggaa catggattcg
     1741 ccggcaaaga agccgccaat cgtcaccccg taaatgccgc cgacgagccg ggggccttgc
     1801 cagcactcaa ggctatgcgc atggccttgc tcgtggagta gcgtgtaggc ctcgatgaac
     1861 tcgggagtga cccacgtgct ccggcggccg ggggcccgcg ctgcgcaacc ctgcattacc
     1921 cgccggaacg cactatccat ggtgatgcga aacacgccct ttcggatgac ccgggcgagg
     1981 ctgcgcgaga cgtgaaagcg atccagctcg aagatggcgc gcggttcggg cgaccaccac
     2041 gtgataggat ccacggtcca ggggaagatg ccgctgcgat aggccagcag caaacggggc
     2101 agagacatgt cgccgccaat cgccaccaac ccctccgcgt cagcacactc cgggctcgga
     2161 aagcgcaacc ggcaatcgag aatggcgatg ggcatgggtc aatgattcgt gatgcgtgat
     2221 gcgtgatgag tgatgagtga cgcgtgcgcc ccgggatcac gcatcacgca tcacgtttca
     2281 cgtttcacct ccgagcgctt cgagcaactg cgcctcaaag taccctgcga tctccatcgc
     2341 ccgatagtgg ggcgcggtct tttcgtttaa ctgcacggac gttttgtcgg gcgagccgag
     2401 cagaacccag cttcccaccc ggatgtcgtt gagcacctgc aagacccatt cgacctcggc
     2461 tggcgggagc gagagccgtc cgcccttttc cgtgggcgcg aaccggcgct gatcggccag
     2521 cagcgcctgc aactgcttct tgttgtccga gcgatgctcc gccagggcct cgtccagcaa
     2581 ctgctggttg gcttccggct ccggcagccg cccggtctgg ctcagctttt ggtgggcggg
     2641 cggcaagcac ggatacaggc tgagaacctc cagaaacagg tgcgcctccc aatagcctag
     2701 atggaagacg agccgggtct tggttgtccg gatcagcttc actcgtcgcg ctccatcgtt
     2761 gcgtgcaagc tgtacttctg aagctggtgc acgtaatgtt ccgccttctc catgctttcg
     2821 cgcgtcaaga gactcttgcc ctgctcgtgg acttgcagca tgtgaaactc ggccttcttc
     2881 cgcggccagc caaacaccac ctggaacgca tgcgtcacat aatccatgag attcaccggg
     2941 tcgttccagc agatcaccag gaagcccggc tccaagccgg acctcggctt ctcctcggtc
     3001 gtttggacga cgccaggctg aatttcgggt gtggccgttg ccgccatagc gccaaactaa
     3061 gagatcagcc cccgcttgtc gagtcgccgg tctgggcaac tggggctcaa attttccttt
     3121 cctatccggg aaatccgtgc aaactgtgtt tcaagcccgg tgagtggcta actcaactgc
     3181 cgaaagtgaa acaatctatg aacacgacct caactcttag agcaatcata atcagcgtgg
     3241 cccttgtggc tatattggct tccaccctgc ctggcatggg ggccgagcaa cccggcaaaa
     3301 tcaaggtgct ccgggtgacg ggcgacgatg cgcagcccgc ccacgactgg cgcgaggttt
     3361 cccaggccct cagggaaacg ctcatctcga gcggcagatt cgacgtgagg atc
//
LOCUS       DATCGS010000239         6527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_516761, whole genome shotgun sequence.
ACCESSION   DATCGS010000239 DATCGS010000000
VERSION     DATCGS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516761"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VIL39_04875"
     CDS             complement(<1..506)
                     /locus_tag="VIL39_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480792.1"
                     /translation="MKLKLLQYDRPALHGGEANTMMSSAGVSLTLSMRAIPPGPLVAV
                     SATTGFGPNFGRGGGSKSGFRPQVANTVLILLKNFCPSSGRGFPSLGRLRTLQAAARQ
                     AARCGSVSWFHRRENVHMYLRARLCAAVWGFLGETRWRRNWPERLRLATTSPRASLCG
                     PLNVFRGSS"
     gene            complement(553..1068)
                     /locus_tag="VIL39_04880"
     CDS             complement(553..1068)
                     /locus_tag="VIL39_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5480793.1"
                     /translation="MGFDGIELIVTARKDLQAFWTDARIDRLKQKLDGHKLRVSQFAM
                     FQPVVEGLSSAQRDVRDQALDYFEGGCRIARKFESPLINIVAPWALELAYCSLVFLLC
                     LPGFSVASAALLSRPRALAGQNNGAAGEHVAPVFPAIHLLHRTDKDARASQGYAAVTL
                     ELLQLFQLLQV"
     gene            1857..2663
                     /locus_tag="VIL39_04885"
     CDS             1857..2663
                     /locus_tag="VIL39_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5480794.1"
                     /translation="MKLYLREIGQVKLLTPEEEIQLAARIKKGDKKAREQMIKANLRL
                     VVKIARDYEGIGLPLLDLISEGNIGLMKAVERFDPAKGGKLSTYGSWWIKQSIKRALA
                     NQSKTIRLPVHLVDKISKMRRTSMRLQEELGREPSDEELGEELGITASRVSQMRMAAI
                     RPASLDAPIGDEDSNNFAEVVQDESADTPYEQLEDKTVTKMLQEMVKTLDNREATILR
                     ARFGLDGGSQQTLEEVGAKFGVTRERVRQIQNIALKKLRKMIEKIEATRT"
     gene            2816..3352
                     /locus_tag="VIL39_04890"
     CDS             2816..3352
                     /locus_tag="VIL39_04890"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY5480795.1"
                     /translation="MSQPRPTAAEIERAIRTIPDFPKPGIQFKDITPVLADARLFSGS
                     IDLLTEKFKPGSVDAVVGIDARGFIFAAAAAVKLQAGFVPVRKKGKLPYKTHEQQYDL
                     EYGTATIAVHTDALKPGSRVLLIDDLLATGGTAAAAATLVKGLGAQILEISFLIELGF
                     LNGREKLKGYPVRSLIVY"
     gene            3368..5620
                     /locus_tag="VIL39_04895"
     CDS             3368..5620
                     /locus_tag="VIL39_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5480796.1"
                     /translation="MHWLQTLDAELFRFINLTLVNPLLDVGMPLASHNALFGPLLVLA
                     VVLLLWKGRARGALCILMLGLILPLGDGMVCNTIKHAIERPRPYAALPDVHRPGTSPS
                     SDSMAPPPPSQETPAAPRAYPPGSLTSMPSSHAANWFAATMILFIYYRRSVRLMLPIA
                     IVVSFSRIYNGVHYPSDVLVGAILGAGYAAAGVWTLNALWQWAGQKWFPLWWTALPSL
                     RAPPRRTESGEDEELPPEDSAPHATVDQHWLRLGYIWIAACLLGRLAYIASKTIQLSQ
                     DEAYQWLWSKHLALSYYSKPPLIAYTQFLGTSLWGDTPFGIRFFAPVIAAVLSLVLLR
                     FFVREVNARAGFFLLLIISATPLMSAGGVLMTIDPLSVLFWTAAMLAGWRAVQPDAPT
                     RPWLWVGLWMGLGFLSKYTELFQLLCWAVFFVLWPPARKHLRRPGPYLALLVNLVCAL
                     PVLIWNAQHEWITVSHVADIAGAHSAWKPTLKYLGEFLGAELGLLNPIFFVATVWAAI
                     AFWRRGRHNPRLVYFFSMGAPVFLAYLAQSFRSSVLPNWIAPSVLPLLCLMVIYWDTK
                     WRLGATKLKPWLVAGLTLGFVLVLLAHDTNLIEKATGWRLPVNQDPLHRVREWSEVAH
                     VAGIARQKLLADGKPVFIIADHYGLVGEISFYLPEAKEAVTKEPLVFYQTSPTPENQF
                     YFWPGYKDRQGQNAIFVRELDRGRTSDPPVPPALEQEFESVTLLGVRNVLYHGRVLRP
                     LQIYACRGLR"
     gene            5626..6501
                     /locus_tag="VIL39_04900"
     CDS             5626..6501
                     /locus_tag="VIL39_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEY5480797.1"
                     /translation="METTKSFTLRDYDLATTLTCGQAFRWEWRDQRWAGVIGTHWVRL
                     HSGPGSLTAETAAPVPDWNWLTDHLQLGVDLTEVTRTFPDDEPMRAALAAARGLRLLR
                     QDPWECLASFILSSTKQIVQIRQIVALLCARYGDPLPVAPACAPAFAFPCPARLARAT
                     EAELRACKMGFRAPYLLATTRIIASGQLDLARLHALPIESARAELMQLPGVGRKIADC
                     VLLFAYGFQAAFPVDVWVMKALQQLYFPRRRVSRQRLRRFAATHFGPCAGYAQQYLFH
                     YMRTKANQPARRAAT"
BASE COUNT         1258 a         2206 c         1893 g         1170 t
ORIGIN      
        1 gatgaccctc tgaaaacatt caacggtcca cagagcgagg cgcggggcga cgtcgtcgcc
       61 agccgcaacc gttccggcca gttcctgcgc cagcgggtct cgcccaaaaa tccccacacc
      121 gccgcgcaaa gccgcgctcg caagtacatg tgcacgttct cgcgcctgtg gaaccagctc
      181 acggagccgc aacgggccgc ctggcgcgcg gctgcctgta acgtcctcag tcggcccaag
      241 ctggggaaac cccgcccgct ggacgggcag aagttcttta acaagatcaa tacggtgttg
      301 gcgacctgcg gccggaagcc gctcttggaa ccgccgccac gcccgaagtt cggcccgaac
      361 ccggttgtcg cgcttacggc caccaatggc cccggcggga tcgccctcat gctcagcgtg
      421 agcgagactc ccgcggagga catcatggtg ttcgcctcgc cgccgtgcaa cgccggccga
      481 tcgtattgca gcaacttcag tttcattggc cttttgcccg ctccgaatga aggaataagc
      541 cacatcacac gtctatactt gaagaagctg aaagagctga agaagctcaa gcgttaccgc
      601 cgcgtagccc tggctggctc gcgcgtcttt atccgtgcgg tgcagcaggt gaatggctgg
      661 gaaaacaggc gctacatgtt cgccagcagc gccgttgttc tggccagcta aggcgcgggg
      721 gcgggacaaa agggccgccg aggcgacgga aaagcccggc aagcacaaga ggaacacaag
      781 agagcaatac gctaactcca gcgcccaggg cgccacgata ttgatcaagg gtgactcaaa
      841 tttccgggcg atccggcagc cgccctcgaa ataatcgagt gcctggtcgc gcacgtcccg
      901 ctgcgcgctg gatagccctt cgaccaccgg ctggaacatg gcgaactgcg acacccgcaa
      961 cttgtgcccg tcgagtttct gtttgaggcg gtcgatccgg gcatcggtcc aaaacgcctg
     1021 gagatccttg cgggcggtga cgatcaattc gatgccgtca aaacccatct cgccgataat
     1081 tttgattgct ggctccgggt cggcggcggg acccaaacta tggaaacacc agctcagaca
     1141 gccgactttg atcgttccgg tgctgcccga ggccgctcgg cgcccactga caagaagggc
     1201 aacgccgatg cagcaagggc ggtagtttgg agaaactggc ggcgatctaa ttctgttcga
     1261 gccttcatgc gcgtaatgcg aattggtttg gcggtgacta agtcgctggt tctattgcat
     1321 aatcccccgc gcaacgtcaa aaccaaattc ttagtgctgc cagcggaata cacggccagt
     1381 gacaaagtcc ttgcgggagc aagaggaggg aggtgagcca aaagccttga gcgccggcgc
     1441 gaagaataga acgggcgcag tgttggctga ctgcgcccgg agaggaaggc tgccgatccc
     1501 acaacctgat ttggtgtcgt tttatttggt tgccaactgc cgcccagtat ttagtctcac
     1561 tcaggtttat ggttgcccac aacaaagctc acaaggtcgc gccgcgaaag cagcgtcctt
     1621 ctacccgccg cacgcgggcg cccaaagcgc acgcgccatc gaaacgccgc gcaccggcga
     1681 cgggaagaag ggaattgccc aggaacaaga aggcattgaa ggccgaggaa ctggtcagag
     1741 aagagaccct caagcccgaa gaagtagaga gggcggctat cacgccggcc cccgtatccg
     1801 aagagccgct tgccccgcgc gagcgggaac gggggagtta tgacggcgac acggcgatta
     1861 agctgtactt gcgcgagatt ggccaagtca aactgctgac gcccgaagag gaaatccagt
     1921 tggccgcccg catcaagaag ggcgacaaga aggcccgcga gcaaatgatc aaggccaact
     1981 tgcgcctcgt cgtgaagatc gcgcgcgact acgagggcat cggcctgcca ctgctcgatt
     2041 tgatcagcga aggcaacatc ggcctaatga aagccgtcga gcgcttcgac ccggccaagg
     2101 gcggcaagct ctcgacctac ggctcgtggt ggatcaaaca atcgatcaag cgagccctgg
     2161 ccaaccagtc caaaaccatc cggctgccgg tgcacctggt ggacaagatc tccaagatgc
     2221 ggcgcacctc gatgcggctg caggaagagt tgggccgcga acccagtgac gaggagctgg
     2281 gcgaagagct gggcatcacc gcgtcgcgcg tctcccagat gcgcatggcg gccatccggc
     2341 ccgcctcgct cgatgccccg atcggcgatg aagactccaa taacttcgcc gaagtcgtcc
     2401 aggacgaaag cgcagacacc ccctacgagc agctcgaaga caagacggtc accaagatgt
     2461 tgcaggagat ggttaagacg ctcgacaacc gcgaggcgac catcttgcgc gcccggttcg
     2521 ggctggacgg cggctcgcag cagacgctcg aggaagtcgg cgccaagttt ggcgtcaccc
     2581 gcgagcgcgt ccggcagatt cagaacattg ccctgaagaa gctgcgcaag atgattgaga
     2641 agatcgaggc gaccaggacc tgagccgcca aaggctcaac acgttccagg aattacatgg
     2701 ggcaaatcag ccgaatcctc cgcgcgcacc taagcaacca cccgacccac catcgccctg
     2761 tgtgcgatga cgattaagac gacaaacctc accacataag aaagaaccaa gcactatgag
     2821 ccaaccccgc ccgacggccg cagagatcga acgcgccatt cgcaccattc ctgacttccc
     2881 caagcccggc atccaattca aggacatcac gcccgtgctg gccgatgccc ggctcttctc
     2941 cggcagcatt gatctgctga ccgagaagtt caaacccggc agcgtggatg ccgtggtggg
     3001 cattgacgcc cgcggattca tcttcgccgc cgccgctgcc gttaagctcc aggccggctt
     3061 tgtcccggtt cgcaagaagg gcaagctccc ctacaagacg cacgagcagc aatacgacct
     3121 cgaatacggc accgccacca tagccgtgca caccgatgcc ctcaagcccg gcagccgcgt
     3181 cctcctcatc gacgacttgc tggccaccgg cggcaccgcc gccgccgccg cgacgctcgt
     3241 taaagggctc ggggcgcaga tcctggaaat ctccttcctg atcgaactcg ggttcctgaa
     3301 cggccgggaa aaattgaagg gctacccggt gcgttcgttg attgtgtatt aagcgcctac
     3361 ttccctgatg cattggctgc aaacgctgga cgctgagctg ttccgattca tcaacctcac
     3421 gttggtcaat cccctcctcg acgtggggat gcccctcgcg agccacaacg ctttgttcgg
     3481 cccgctgctg gtgctggcgg tcgtccttct cctctggaaa ggccgcgccc ggggcgcgct
     3541 gtgcatcctg atgctgggac tgatcctccc gctcggtgac gggatggtct gcaacaccat
     3601 caaacatgcc atcgagcgcc cgcggcccta tgccgccctg cccgatgttc atcgccccgg
     3661 aaccagtccc agctccgata gcatggcacc gccgcctccg tcccaggaaa cgccggccgc
     3721 gccacgcgcc taccccccgg gcagtctcac cagcatgccc tcatcccacg ccgccaattg
     3781 gtttgcggcc acgatgatcc tcttcatcta ctaccgccgt agcgtgcggc tcatgctgcc
     3841 catagccatc gtggtcagct tttcccgcat ctacaacggc gtccattacc cgagtgacgt
     3901 cctcgtcgga gcgatcctgg gcgcgggtta cgccgccgcc ggggtctgga ctctcaacgc
     3961 gctctggcaa tgggccgggc agaaatggtt tccgctctgg tggaccgcgc tgccgtccct
     4021 gagggctccc ccccggcgaa ccgaatcggg cgaagatgaa gaattgcccc cggaggattc
     4081 ggctccgcac gccacggtgg accagcattg gctccgcctc ggttacatct ggatcgcagc
     4141 ctgcctgctg ggacggctgg cctacatcgc cagcaagacc attcaactca gccaggacga
     4201 agcctaccaa tggctctggt ccaaacacct cgcgctctcc tactacagca agcctccgct
     4261 catcgcctac acgcagttcc tcggaacctc cttatggggc gacaccccct tcggcatccg
     4321 gttctttgcc ccagtcatcg ccgcggtcct cagcctcgtc ctgcttcgat tcttcgtgcg
     4381 tgaggtcaac gcccgggccg ggttcttcct gctgctcatc atcagcgcca ccccgctgat
     4441 gtcggctggc ggggtgctca tgaccattga tccgctctcc gtcttgttct ggacggccgc
     4501 catgctggcc ggctggcgcg cggtgcagcc ggacgcgccg acccggccct ggctttgggt
     4561 cggcctctgg atgggcctgg gctttctgag caaatacact gagctattcc agttgctttg
     4621 ctgggccgtc ttttttgtcc tctggccgcc cgcgcgcaaa cacctgcgcc gcccggggcc
     4681 atacctggca ttgctggtca acctggtctg cgccctgccc gtgttgattt ggaacgcgca
     4741 gcacgaatgg atcaccgtct ctcacgtcgc cgatatcgcc ggcgcccata gcgcgtggaa
     4801 acccaccctg aaatacctgg gcgaattcct tggggccgag ctcggtctgc tcaacccgat
     4861 cttcttcgtc gccacggtct gggccgccat cgccttctgg cgtcggggcc gtcacaaccc
     4921 tcgcctcgtt tatttcttca gcatgggcgc gccggtcttc ctggcttact tggcacagtc
     4981 atttcggtcg agcgtgctgc ccaactggat tgcgccctcg gtcctgcccc tgctctgcct
     5041 gatggtgatc tactgggaca cgaagtggcg gctcggcgcg acaaaactca aaccctggct
     5101 ggtcgccggc ctcacactgg gcttcgtcct cgtcctcctc gcacacgaca ccaacctgat
     5161 cgagaaggcg accggctggc ggctgcccgt caaccaggac ccgttgcacc gcgtgcgcga
     5221 atggagcgaa gtcgcccacg tcgccgggat cgcccggcaa aagttgctcg cggatggaaa
     5281 gcccgtgttc atcatcgctg atcattatgg gctggtcgga gagatttcat tttacctgcc
     5341 ggaagccaaa gaagccgtca ctaaggaacc cttggtcttc taccaaacca gcccgacccc
     5401 cgaaaaccag ttctacttct ggcccggcta caaagaccgc caaggccaga atgccatctt
     5461 cgtccgcgaa ctggaccgcg gccgaacgag cgatccaccg gttccaccgg cgttggagca
     5521 ggaattcgag tcggtgacgc tattgggcgt gcgcaatgtc ctctatcatg gccgggtgct
     5581 gcgcccgctc cagatttacg cctgccgcgg cttgcggtaa tcggcttgga aaccacgaag
     5641 tccttcacgc tgcgcgacta cgacctggcc accacgctca cctgcggcca ggcattccgc
     5701 tgggaatggc gcgaccagcg ctgggcaggc gtgatcggaa ctcactgggt ccgactgcac
     5761 tccggccccg gctcccttac ggccgagacc gccgcgccgg ttccagactg gaattggctg
     5821 accgatcacc tgcaactggg cgttgacttg actgaggtga cgcggacctt tccggatgac
     5881 gagccgatgc gcgccgccct cgccgccgct cgcggcctgc gtctgttgcg ccaagacccc
     5941 tgggaatgcc tggcttcctt catcctctcc tccaccaaac agatcgtcca aatccgacag
     6001 atcgtcgccc tgctctgcgc acggtacggc gacccgctcc cggtggcgcc cgcctgtgcg
     6061 cccgcgttcg ccttcccctg ccctgcccga ctggcccggg ccaccgaggc cgagctgcgc
     6121 gcctgcaaaa tgggattccg cgcaccgtat ttgctggcga ccacgcgcat aattgccagt
     6181 ggccagttgg acctggcccg cctgcacgcg ttgccgatcg agtccgcccg cgccgagctg
     6241 atgcaactgc cgggtgtggg ccggaagatc gctgattgtg tcctcctgtt cgcctacggc
     6301 ttccaagccg cgttcccagt ggacgtctgg gtgatgaaag ccttgcagca actctacttc
     6361 cctcgccgtc gagtcagccg ccaacgcctc cgtcgctttg ccgccaccca cttcggcccc
     6421 tgcgccggtt acgcgcaaca gtatctgttc cattacatgc ggacgaaagc gaaccagcca
     6481 gccaggcgcg cggcaacgta gccattcaga ctcaggggac cgcgccc
//
LOCUS       DATCGS010000240         4537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_20047, whole genome shotgun sequence.
ACCESSION   DATCGS010000240 DATCGS010000000
VERSION     DATCGS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20047"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..3145
                     /locus_tag="VIL39_04905"
     CDS             341..3145
                     /locus_tag="VIL39_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY5480798.1"
                     /translation="MGGHSQARRVCAAAIGSLLLGAGLAGAESADRQGLFPVQSTVVL
                     VAGVPGDVESEENYRDQMQGWLALVTGSGQAARIFVLCDDPQAVTLPESSDGKSAPKE
                     GEVFTNHPPADQSPISNRQLPIANHPAQVSVLKGDRSSFLGLGQKLAGGSNALVVIAW
                     GHGGRQGNTPVLHVRGPRITAADFKAVAHQAAAGESRWVLMFQGSGSFANELAGERRQ
                     IISSEGEMMFSDDPVGMPLLLKLAKEKPELPFQGLAEELGRATEAWYRDRNLARTEEP
                     TLWLATDKPRHLVVLAAEAEAGEAMKPEEPKPGATNAPSAEPAFVAVVPGDLPAVWKE
                     IKRVEARKYAEADAVILRRRLSYTLGSSPAIATEQEEFVQVLTPEGKRYGDFDVSYSP
                     PHEDITFLDCEVLRPDGKLVRLDPDAIRDAREQAVGDYQRAQRKFFSLPGVGPGAVLH
                     VRYRTIWKTFPLPHVSLEIPVGQDLATLEATVQVSVPKETAFHFAPDQVAAADPVLKQ
                     TTYGTTYSWHFENLPAQQREILVSPGQRSRLLISTFPDWAGFGEWYGRISKLADEVTP
                     EITAKAKELTKDTNGDREKVLALYNYVTRLRYVAVPMGVNSFRPHAAANVLQNEFGDC
                     KDKANLFNTLLRSVGIEARLVLVPRFSQAHEGIPGLAFNHAISRVTLGGETVWVDTTD
                     DVCRFGMLPPGDPGRKVLVIGGETNALTQLPAPDPREHVLKVRGEVDCSGPMDALPVK
                     LSATALGYPDYELRTAAREAKEHAFALPLLAAKFHPAAGSFALESQSATSVAALNEDF
                     TWRAAGTCVGLFSKAGSGGLLRAPFWLPKEWDLALHRRKAGLYLNLGYPLTLEQEFEF
                     ALPPKTQPAVLPGVKENKTAPLRWRVEWTKIGDDKVLARLRAELARGELTVAEMPMVQ
                     RQLRELLAALAAGASLPAAP"
     gene            3317..4360
                     /locus_tag="VIL39_04910"
     CDS             3317..4360
                     /locus_tag="VIL39_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5480799.1"
                     /translation="MTDQSTLHTVHEVPPVIERDPAEVLDQLAPKIRLLKGEMAKVIV
                     GQEDVIMAALYALFSRGHCLLVGVPGLAKTLLVQTLARSLELAFSRIQFTPDLMPSDI
                     TGTEILEEDAATGHRRFVFGQGPVFTQVLLADEINRTPPKTQAALLEAMQEKTVTVAG
                     KTYHLEEPFMVLATQNPIEQEGTYPLPEAQLDRFLFEVLVEYPTLEEENKVVAQHSFT
                     PLVGLQPVLTRDEVLSFRRAIAQVPAAPNVIEYAVRLVRATRPSSDGEAPDYIKRWIR
                     WGASPRASQYLILAGRARAACDGRFNVACEDVAALAPLVLRHRLIRTFQADAEGKTTD
                     EIIGQLLQDVAAG"
BASE COUNT          849 a         1202 c         1681 g          805 t
ORIGIN      
        1 actcggcgga tcacccgcgg cacggcgcag ggttcaaccg gaaaacgaga ggagaacaag
       61 cagaacacaa tggagtaacg ctaccccaag gctaccccaa agccggctga gcttatgtca
      121 aacccgagat ctgaaggccg aatcaatagc cgcccaaggt gggggagcgc gcctccactc
      181 aaacatgctt tggatttggg ctttcgagtt gcggtcttcg gatttgaggg ctgctggctt
      241 cggccaccct tcgaccgggc ggccttcgcc ggtggtgcgg ggggaacgtt tttggtttcg
      301 tatggtctaa agaacggcta tatccggata caaagtttct atgggcgggc actcgcaggc
      361 gcggcgtgta tgcgcagcag cgatagggag tcttctcctg ggcgcgggct tggcgggggc
      421 cgaatcagcg gaccggcagg ggcttttccc ggtacaatcg acggtggtgc tggtggcggg
      481 ggtgccgggg gatgttgaga gcgaggagaa ttaccgggat cagatgcaag gctggttggc
      541 cctggtgacg ggcagcggcc aggcggccag gatcttcgtg ctgtgcgatg atccacaggc
      601 ggtgacgctg ccggagagct cggacgggaa gagcgctcca aaggaagggg aggtgtttac
      661 caatcaccct cccgctgacc aatcaccgat tagcaatcgc caattgccaa tcgccaatca
      721 ccccgcccaa gtcagcgtcc ttaagggcga tcggagcagc tttctgggtc ttggccagaa
      781 gctggcgggc gggagcaatg cgctggtggt gattgcgtgg ggccacgggg ggaggcaagg
      841 gaatacgccg gtgctgcatg tgcgggggcc gcggatcacg gcggcggatt tcaaggcagt
      901 ggcgcaccag gcggcggcgg gggagtcgcg ctgggtgctg atgttccagg ggagcggatc
      961 gtttgcgaac gagttggcgg gggagaggcg gcagatcatt tcctcggagg gcgagatgat
     1021 gttcagtgac gatccggtgg ggatgccgtt gctgctgaag ctggcgaagg agaagccgga
     1081 actgccgttc caggggttgg cggaggagtt gggtcgagcg acggaggcgt ggtacaggga
     1141 ccgtaacctg gcgcgcaccg aggagcccac gctttggttg gcgacggaca agccgcgtca
     1201 tctggtcgta ctggcagcgg aggcagaagc cggcgaggcg atgaagccgg aggaacccaa
     1261 gccaggggcg accaatgccc ctagtgcaga accggcgttc gtagccgtgg tgccgggcga
     1321 cctgccggca gtttggaagg agatcaaacg ggtcgaggcc cggaagtatg ccgaggccga
     1381 tgcggttatc ctgaggaggc ggttgagcta cacgctgggc agcagcccgg cgattgcgac
     1441 ggagcaggag gagttcgtgc aggtgctgac gccggagggg aagcgttatg gcgatttcga
     1501 cgtgagctat tcgccgccgc acgaggacat tacgttcctt gactgcgagg tcttgcgtcc
     1561 ggatggaaag ctggtgcggc tggacccgga cgcgattcgc gacgcgcgcg agcaggcggt
     1621 aggcgattac cagcgggcgc aacgcaagtt cttctccctg ccaggggtag gaccgggcgc
     1681 ggtgctgcac gtgcgttacc gaacgatctg gaagacgttc ccgctgccgc acgtgtcgct
     1741 ggagataccg gtcgggcagg atctggcgac gctggaggcg acggtgcaag tgagcgtgcc
     1801 aaaggagacc gccttccact tcgcgccgga ccaggtggcc gccgccgatc cggtccttaa
     1861 gcagacgact tacgggacga cctactcgtg gcatttcgag aatctgcccg cgcagcagcg
     1921 cgagattctc gtctcgccgg ggcagcggtc gcggctgctg atctcgacgt tccccgattg
     1981 ggcggggttt ggggagtggt atgggcggat cagcaagctg gcggatgagg tgacgccgga
     2041 gatcacggcc aaggcgaagg agttgacgaa ggacacgaac ggggaccggg agaaggtgct
     2101 ggcgctgtac aattacgtga cccggctgcg ctacgtggcg gtgccgatgg gggtcaattc
     2161 gttccggccc catgccgcgg cgaacgtgct ccagaacgag ttcggggatt gcaaggacaa
     2221 ggccaacttg ttcaacacgc tgttgcgctc agtggggatc gaggcgcgcc tggtgctggt
     2281 gccgcgcttt agccaggcgc acgaggggat cccgggtctg gctttcaacc atgccatttc
     2341 gcgggtcaca ctgggcggcg aaacggtgtg ggtggatacg acggatgacg tttgccgttt
     2401 cgggatgctg ccgccgggcg atccggggcg gaaggtgctg gtgatcgggg gcgagactaa
     2461 tgcgctgacg caattgccgg cgcccgatcc gcgcgagcac gtgctcaagg tgcgcgggga
     2521 ggtggattgc tccggcccga tggacgcgct gccggtcaag ttgagcgcga cggccctggg
     2581 gtatccggac tacgaactgc gcacggcggc gcgggaagcg aaggagcacg catttgcgct
     2641 gccgctgctg gccgcgaagt ttcatccggc agcaggctcc tttgcgctgg agagccaaag
     2701 cgcaacctcg gtggccgcgc tcaatgagga tttcacctgg cgcgccgcgg ggacgtgcgt
     2761 gggactgttc tcaaaggcag gaagtggcgg gttgctgcgc gcgccattct ggctgcccaa
     2821 agagtgggac ttggcgctgc atcgccggaa ggcggggctt tacctgaatc tggggtaccc
     2881 gctcacgctg gagcaggagt tcgagttcgc gctgccgccg aagacgcaac cggcggtcct
     2941 gcccggggtt aaagagaata agacggcgcc gttgcgttgg cgggtcgaat ggaccaagat
     3001 cggcgacgac aaagtgttag cgcggctgcg ggctgagctg gcgcgcgggg agctaacggt
     3061 ggcggagatg ccgatggtgc agcggcaact gcgcgagctg ctggcggcgc tggctgcggg
     3121 cgcgagtttg ccggcggcgc cataggaagc tggcgttagg aatgcggtga aaccacgaaa
     3181 tacccagcgt ggcagagcga caaccgaaga tagccaatag ccaatagcaa atggccgatg
     3241 cgctgcggca cctgagaatt gcgcaggctg cgcaaactgt gacggatagt agtacaaagc
     3301 acacgaaaag acaactatga ctgaccaatc cacacttcat accgttcacg aagtgccgcc
     3361 ggtaatcgaa cgtgatcccg ccgaggtgct cgaccagctc gcaccgaaga ttcgattgct
     3421 caagggtgag atggccaagg tgattgtcgg gcaggaggat gttatcatgg cggcgctgta
     3481 tgcgctcttt tcccgcggcc actgtttgct ggtgggcgtg cccggtttgg ccaagacgct
     3541 gctggtgcag acgctggcgc gttcgctgga actggccttc tcgcgcattc aatttacgcc
     3601 cgacctgatg ccgtcggaca tcaccgggac ggagatattg gaggaggacg ccgcgaccgg
     3661 gcaccggcgg tttgtgttcg ggcaggggcc ggttttcacg caggtgctgc tggcggatga
     3721 aatcaaccgc acgccgccca agacgcaggc ggcgttgctg gaggcgatgc aggagaagac
     3781 cgtgacggtt gccggcaaga cgtatcatct ggaagagccg ttcatggtgc tggcaacgca
     3841 gaatccgatt gagcaggaag ggacctaccc gttgcccgaa gcgcaactgg accggttcct
     3901 gttcgaggtg ctggtggagt atcccacgct ggaggaggag aacaaagtgg tggcgcagca
     3961 ttccttcacc ccgctggtcg ggcttcagcc ggtgctgacg cgcgatgaag tgctgagctt
     4021 ccgtcgggcc atcgcgcagg tgcccgcggc gccgaatgtg atcgaatatg cggtgcggct
     4081 ggtgcgggca acacggccat cgagcgacgg cgaggcgccg gattacatca aacgctggat
     4141 taggtggggc gcgagtccgc gcgccagcca gtacctgatc ctcgcgggcc gggcgcgggc
     4201 agcctgcgac gggcgtttta acgtggcctg cgaagacgtc gcggcgctcg cgccgctggt
     4261 gctgcggcat cggttgattc ggactttcca ggcggatgca gagggcaaga cgacggatga
     4321 gatcatcggg caactgttgc aggatgtggc ggcggggtag gggggaggag ttgatggtgt
     4381 tgaatgagtt gatggagtta catgagtcgc atggattggg cgcacttgct gagatgaaga
     4441 ttgagcatcc attcttgcat tggctgtggc aggccgcagt attcctcgct tgttgctttg
     4501 gcaccgtcgt actcatgcag ttgttgatgt ccaagta
//
LOCUS       DATCGS010000241         3746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_417725, whole genome shotgun sequence.
ACCESSION   DATCGS010000241 DATCGS010000000
VERSION     DATCGS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417725"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VIL39_04915"
     CDS             <1..1094
                     /locus_tag="VIL39_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY5480800.1"
                     /translation="LWTLVNRTEYDIPGPQLRVPHETGRRYFDLWHGVELTPAFTTNQ
                     GVRSANLSFEIEGRGYGAVLAWDNESLNADFQPLLARMKAMSGKPLRAFSSEWKFLPQ
                     TQVPIKAVRAPRNPPGDMVRIPGGGFEFVVSGIMVEGGDGVGVDVQYPWEDSPRRHHR
                     QQLNLKPFWIDRYPVTNARFKEFLDATGYQPRDSGNFLKDWKDGTFPAGWGNKPVTWV
                     AIEDARAYAHWAGKRLPHEWEWQYAAQGPDRRLFPWGNEPNAAAVPAPDKSRSPRPPT
                     EVEAFPGGASPFGVMDMAGNVWQWTDEYLDEHTRAAVVRGGSYFFPQGSGWYFPNSSR
                     LTEHGKLLLMAPCKDRAATLGFRCVMDAN"
     gene            1182..1409
                     /locus_tag="VIL39_04920"
     CDS             1182..1409
                     /locus_tag="VIL39_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480801.1"
                     /translation="MKLKAHNQRSLAKGQIWKTRAADIEIVALGNELIHYRITKQFGL
                     KGVSAQISGIEAMANYLATNEARLVKGPSTN"
     gene            1536..2090
                     /locus_tag="VIL39_04925"
     CDS             1536..2090
                     /locus_tag="VIL39_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480802.1"
                     /translation="MEPRAFLFWQIPLDPTVISSRRFRAEAPSVLMDTTLSRLRVWPL
                     WQKSKHFVVSGVHEDSATGARADEFCRTLSQCLGRNCEFTKELWLLTELRTASLRAIA
                     AKEAASADLVIISVHHGEKLPTEVKSWVDLWLKQKGRRSTVLLALFDPPYLGTSSSIQ
                     ACLQQAAERGNMEFLARSEEKLED"
     gene            2155..2445
                     /locus_tag="VIL39_04930"
     CDS             2155..2445
                     /locus_tag="VIL39_04930"
                     /inference="COORDINATES: protein motif:HMM:NF033216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HEY5480803.1"
                     /translation="MKKANLPLLIGLIALTGCAHHYVMKLTGGSEITTASKPRLKDGI
                     YHFKDAKGEEHFIPEGRVREVAPASVVARENKPQPIKAPSVKKRKWYLLWLG"
     gene            complement(2492..3103)
                     /locus_tag="VIL39_04935"
     CDS             complement(2492..3103)
                     /locus_tag="VIL39_04935"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5480804.1"
                     /translation="MFTGIVEETGTVERIKPAAKSIELTVRANLCGRGLKVGASLAIN
                     GCCLTVVKVASPGKSRLVQFDLLKETWVRTNLQFAKPGSLVNLERPLRTDGNLGGHFV
                     TGHIDGIGKITRWERMGQDHVLDIAAPPEVMRYVVFKGSMAVDGISLTVAGVQKKSFR
                     IWIIPHTYEITALRERKVGDAVNLEADLIGKYVEKFVTARACR"
     gene            complement(3329..>3746)
                     /locus_tag="VIL39_04940"
     CDS             complement(3329..>3746)
                     /locus_tag="VIL39_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5480805.1"
                     /translation="PVEAKVASDECAALTTIVVSKLAPKRRVAALAKRVRVLVAPSAD
                     GKINLRWLLKKLGSENVTRMLVEGGGEVNASFLLHGLAQRVTFFYAPMILGGRDSRRG
                     VAGAGARTLAESLSLADLEWQRLGPDWLLSAQVVAR"
BASE COUNT          739 a         1180 c         1103 g          724 t
ORIGIN      
        1 cgctttggac actcgtcaac cgcacggaat acgacatccc cggcccgcaa ctgcgcgtgc
       61 cgcacgaaac cgggcggcgc tatttcgatc tgtggcacgg cgtcgagctg acgcccgcct
      121 tcaccaccaa ccagggcgtc cggagcgcca acctgagctt cgagatcgaa ggccgcggtt
      181 acggggccgt gctggcgtgg gacaacgaat cgctcaacgc ggactttcag ccgctgctgg
      241 cccgcatgaa agcgatgtcc ggaaaaccgc tccgcgcctt ctccagcgaa tggaagttcc
      301 tgccgcaaac gcaggtgccg atcaaggcgg tgagagcccc caggaatccg ccgggcgaca
      361 tggtccgcat tcctggcggc gggttcgagt tcgtggtctc cggcatcatg gtggaaggtg
      421 gcgacggggt cggtgtcgat gtgcagtatc catgggaaga ctcgccgcgg cgtcaccatc
      481 ggcagcagct caacctcaag ccgttctgga tcgaccgtta tccggtgacg aacgcgcggt
      541 tcaaggaatt cctcgatgcg accggctacc aaccgcgcga tagcggcaac tttctcaagg
      601 actggaagga tggcactttc ccggccggct ggggcaataa accggtcacc tgggtcgcca
      661 tcgaagacgc tcgcgcctac gcccactggg cggggaaacg cctgccgcac gaatgggaat
      721 ggcaatatgc cgcccaaggg cctgaccggc gcctgttccc ctggggcaac gaaccaaacg
      781 ccgccgccgt gccggcgccc gacaagagcc gcagcccgcg tccgccgacg gaggtcgagg
      841 ccttcccggg cggcgccagc ccctttggcg tgatggatat ggccggcaac gtgtggcagt
      901 ggacggacga gtatctggac gagcacacgc gcgccgccgt ggttcgtggt ggcagctact
      961 tcttcccgca aggctcgggg tggtatttcc cgaactcctc ccggctcacc gagcacggca
     1021 agctgctgct gatggccccc tgcaaagacc gcgcggcaac gctcgggttc cgctgtgtca
     1081 tggacgcgaa ttagcctgat tggtccaccg atggctctgc gtgcgggagg acgtcgagca
     1141 gccaggttga acaaataagg tattccaccg tctaagacag gatgaaactg aaagctcata
     1201 accagcggtc gttagccaaa ggccagattt ggaaaacgcg agccgcggac attgagattg
     1261 tggcgctggg taacgagctc atccactacc ggataaccaa gcagtttggc ctcaaaggag
     1321 tcagtgcgca gatcagcggc attgaggcta tggccaatta cctggcaacc aatgaggcgc
     1381 gcctggtgaa gggtccctcc actaattgat ttctcccatg attgctgctg ccagggtcga
     1441 aatggctgcc gccgccgggg cccggtggac gcccgtgaac cccaccgcaa tcaactcgct
     1501 gtagcctgct ttctttcaac aaggcatgcg gctttgtgga gccgcgtgcc tttttatttt
     1561 ggcagattcc acttgaccca actgttattt caagcagacg attccgcgca gaagcaccgt
     1621 ctgttctcat ggacacaaca ctatcaaggc tgcgcgtgtg gccgctctgg cagaaatcga
     1681 agcacttcgt cgtttcgggg gtgcacgaag attctgccac aggcgcccgc gcggacgaat
     1741 tctgtcggac actctcccag tgcctcggcc ggaattgtga attcaccaag gagctgtggc
     1801 tcctgactga gctgcgcacg gcttcattga gagcgattgc ggccaaggaa gcagcttccg
     1861 ccgacctggt tatcatttcc gttcaccatg gcgagaagtt gccgactgag gtcaagagct
     1921 gggttgacct gtggcttaag caaaaaggca ggcgttcaac cgtccttctg gccctctttg
     1981 atccgccgta tctgggcacc tccagttcga ttcaagcctg tttgcagcaa gcggctgaaa
     2041 gaggaaacat ggaattcctg gctcgctcag aggagaagct ggaagactga acatggctca
     2101 gggcgcttcc aacagacctt ctcccgccgt ctcgcaatgc tctcttgtgc tgcaatgaaa
     2161 aaagcaaacc tgccccttct catcggcctg atcgcgctga ccgggtgcgc gcatcattat
     2221 gtgatgaaac tcaccggtgg ctcggagatt accaccgcca gcaaaccgcg gctcaaggac
     2281 ggcatttacc atttcaagga cgccaagggt gaggagcatt tcataccgga ggggcgtgtc
     2341 cgcgaggtcg cccccgcctc ggtagttgcc agggagaaca agcctcaacc aatcaaggcg
     2401 ccgagcgtga agaagcgaaa gtggtatctg ctgtggctgg gataatcttc tccagctcac
     2461 tcctggccgg caaggaggct gacttggcgc ctcagcggca cgcccgggcg gtgacaaact
     2521 tctcgacgta tttgccgatc aggtcagctt ccaggttgac ggcgtccccc actttgcgct
     2581 cgcgcagggc ggtaatttcg taagtgtgcg ggatgatcca gatgcggaaa ctcttcttct
     2641 gcacgccggc gacggtgagg ctgatgccat cgaccgccat tgagcctttg aacaccacgt
     2701 agcgcattac ctcgggtgga gcggcaatgt ccagcacgtg gtcctgcccc attcgctccc
     2761 accgcgtgat cttgccaatg ccgtcaatgt ggccggtcac gaaatgtccg cccaggttgc
     2821 catccgtgcg cagcggtcgc tccagattga ccagcgagcc aggtttggcg aactgcaggt
     2881 tcgtccgcac ccaggtttcc ttcagcagat cgaattggac cagcctggac ttgccgggtg
     2941 aagccacctt caccaccgtc agacagcagc cgttgatcgc caggctcgct cccaccttca
     3001 gcccccgccc gcagaggttg gcccgcacgg ttagctcgat ggacttcgcc gccggtttga
     3061 tccgctcgac ggtgcccgtt tcttcaacga ttccagtaaa catagccgca tactaaaccg
     3121 tatccatgca gaagagaacc acagatggac acagattaac acagattctg ggggccagac
     3181 ttccgtcttg cttccctttg ctttaggcca ctgtccggta cacggagttc tgccatggaa
     3241 gggggcggtt ttgagcagtg cttatctgtg ttcatctgtg tccatctgtg gttgtcttga
     3301 attggcgcgg ccaagtccga aacccgaatc agcgggcaac aacctgggcg ctcagcagcc
     3361 agtcgggacc cagccgctgc cactccagat cggcaaggct cagactctcc gccagcgtcc
     3421 tcgccccggc gccggcgacc cctcggcgcg agtcgcgccc gcccaaaatc atcggcgcgt
     3481 agaagaacgt gacgcgctgt gccaggccgt gcagcaggaa tgatgcgttc acttcgccgc
     3541 ccccttcgac cagcatgcgg gtgacgtttt ccgagccgag cttcttcaac agccagcgca
     3601 ggttgatctt gccatcagcg ctcggcgcga ccagcaccct gacccgcttt gccaatgccg
     3661 ccacgcgcct tttcggggcc agcttgctca cgacaatcgt cgtgagggcc gcgcattcat
     3721 cgctcgcaac cttcgcctcc accggc
//
LOCUS       DATCGS010000242         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_121195, whole genome shotgun sequence.
ACCESSION   DATCGS010000242 DATCGS010000000
VERSION     DATCGS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121195"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VIL39_04945"
     CDS             complement(<1..579)
                     /locus_tag="VIL39_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5480806.1"
                     /translation="MRLAILTLLSVPLWAQTPLSLKDAVRVALDKHPSMEAAGAQVTA
                     AEMRARQARGGFLPKVNYTESWQRSDNPVFVFSSLLTQHQFSEQNFAIGPLNRPDAMN
                     NFQSQVALDQTVWDAGMTSKQVRSAELGGKMAGEEQRRTSMDLIAGVVRAYEGVLLSQ
                     ESLKVAREAVRSAAADLERAQAVQSAGMATDAD"
     gene            701..1111
                     /locus_tag="VIL39_04950"
     CDS             701..1111
                     /locus_tag="VIL39_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5480807.1"
                     /translation="MEASITYLDGVRFEAESRGHRVICDQPAGSSGADTGMSPPEFLL
                     VSLGTCAGFYAVQYLKTRSLPTEGLAVRVLAEKASHPVRLASFRIEVTVPGLDPRHHE
                     GILRAVKLCLVHNTLLQPPAIETVLKTEARAAVV"
     gene            complement(1144..2451)
                     /locus_tag="VIL39_04955"
     CDS             complement(1144..2451)
                     /locus_tag="VIL39_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5480808.1"
                     /translation="MSVLSHTGRYLAGVEGVFRAGHWTRSQLQDYQAARLRALVRHAS
                     RKVPFYRRRLEAAGLDWRQVRGIEDLRRLPVVQRSELRTATPDELLADGADGAELVTH
                     LTSGTTGHPLAVRRTWVEERLLNAFRLQVLFGYGMRWRDLRTSLRLSGSIERSWWARL
                     GLLRQHSLSVYDGWESLKAQILASKPEVLEGYAATTAAVADLMTGDDRKGLGLKFVAA
                     GAEAVTTERRRRITDGFGARVYECYGSVEFNLIAAECRQNGLMHICEGSVLVEVLRDG
                     HPVQAGEEGEAVITALHSYAMPLLRISLGDRVRAGPAPCPCGAPFGTLERVEGREDPL
                     FPLPDGRVLPAYLLRLPLVERWPWVRQYQLAQERLDLLRLKVVPVDGVTDVAERLQDW
                     LRFSREQCGYQVQIVIEQVSEIPLGTGGKFQQLVSKVQGEAKS"
     gene            2722..>3351
                     /gene="rlmB"
                     /locus_tag="VIL39_04960"
     CDS             2722..>3351
                     /gene="rlmB"
                     /locus_tag="VIL39_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY5480809.1"
                     /translation="MAILVGIHAVLEALRAGRSFDRVVVSKGAGGERIQEVVDLCRSA
                     SVPTRFEPRASLDRLAGSSAHQGVVALGAAHRYATIEDIPSGAKLLVVLDGVEDPHNL
                     GAVIRTAHAAGAAALVIPERRAVGITEVVAKAAAGALEYLPVVRVSNLNRALETLKER
                     GFWIYGFDERGQSIYDETDFASPTVFVLGGEGQGLHHQVREHCDLVMRIP"
BASE COUNT          594 a         1139 c         1022 g          596 t
ORIGIN      
        1 atcggcatcg gtggccatcc cggcggactg cacggcctgc gcacgctcga gatcggccgc
       61 cgccgagcga accgcctcgc gcgccacctt cagactttcc tggctcaaca ggacgccttc
      121 ataggcgcgc actaccccgg cgatcagatc catgctggtt cgccgctgct cttcacccgc
      181 cattttcccg ccgagttcgg ccgaccgaac ctgcttggag gtcatccccg cgtcccaaac
      241 cgtctggtcc agggccacct gcgactggaa gttgttcatg gcatcgggac gattgagcgg
      301 gccaatggcg aaattctgct cgctgaactg gtgttgcgtg agtagcgagc tgaacacgaa
      361 gaccggattg tcgctgcgtt gccaggattc ggtgtagttc accttcggaa ggaatccgcc
      421 gcgggcctgg cgcgcgcgca tctccgcggc ggtaacctgc gcgccggcgg cctccatcga
      481 ggggtgctta tcgagcgcca cgcggactgc atctttgagc gataggggtg tctgcgccca
      541 caacggcacc gacagcaggg ttaatatggc taatctcatg cggttcacgg ctccaagggc
      601 ctcgtctctg aactaagatg caggagggag ccggacgtaa cagcgcaatc ttcccgtcac
      661 cttcggccag cggctgcatc tcagaggatg aggtcctttt atggaagcga gcataaccta
      721 tttagatggt gtgaggtttg aggccgagag ccgcgggcac cgggtcattt gcgaccagcc
      781 ggccggcagc tccggggcgg acaccgggat gagcccgccg gagtttctgc tggtgtccct
      841 gggtacctgc gcgggattct acgcagtcca atatctgaag acacggagct tgcctacgga
      901 ggggctggcg gttcgcgtcc tggccgagaa ggccagccat ccggtccggc ttgcttcctt
      961 ccgcatcgaa gtcacggtgc cgggactgga tccccggcat catgagggca tcctccgcgc
     1021 cgtgaagctg tgcctggtcc acaacacgct gctgcaaccg cccgccatcg aaacggtgct
     1081 caagaccgaa gcccgggccg ccgtcgtgtg atcggcacac gggctactga ggaagtgccg
     1141 gcatcaggac ttcgcctctc cctgcacctt cgagaccaat tgctggaact tgccgcctgt
     1201 gccgaggggg atctcggaga cctgttcgat gacaatttgc acctggtagc cgcactgctc
     1261 tctcgagaag cgcaaccagt cctgcaaccg ctcagcgacg tccgtcaccc cgtccaccgg
     1321 gacgaccttc aagcgcagca gatccaggcg ctcctgcgcc aattggtact gtcgcaccca
     1381 cggccaacgc tccacaagcg gcagtcgcag aaggtaagcc ggcagcaccc ggccgtcggg
     1441 cagcggaaac agcggatctt ctcttccctc cacccgttcc agtgttccga acggagctcc
     1501 gcaggggcat ggcgcaggcc ccgcgcggac tctgtcaccc agcgaaatcc gaagcaaagg
     1561 catggcgtag gagtgcagcg cggtgatgac tgcctccccc tcttcgccgg cctgaacggg
     1621 atgcccgtcc cgcaacactt caactaagac gctcccctcg cagatgtgca tcaaaccgtt
     1681 ctggcggcac tccgctgcaa tcaggttgaa ctccacgctg ccgtaacatt cgtacactct
     1741 ggctccgaag ccgtccgtga tcctgcgccg cctctccgtc gtcacagcct ctgcgcccgc
     1801 agcgacgaac ttgagcccaa gaccctttcg gtcatcgccg gtcatgagat cggccacggc
     1861 agccgtagtg gccgcatatc cttccaacac ttcgggtttc gaggccagga tttgggcttt
     1921 cagggattcc cagccatcgt agacgctaag cgaatgctgc cgcaggagac cgagtctggc
     1981 ccaccaggag cgttctatgc tgcccgaaag ccgcaggctg gtccgcaggt cccgccaacg
     2041 catgccgtag ccgaacaata cctgcaacct gaaggcgttc aacagccgct cttcgaccca
     2101 cgtgcgacgc acggccagtg gatgccccgt cgttccgcta gtgaggtgcg taaccagttc
     2161 agcgccatcc gcaccgtccg ccagaagctc atcgggcgtt gcggtgcgca actcggaccg
     2221 ctgcaccaca ggcaagcgcc tcaaatcctc gattcccctg acttggcgcc agtcgaggcc
     2281 cgccgcctcc agtcgccgcc ggtagaacgg caccttccgc gaagcgtggc gcaccagggc
     2341 acgcaggcgg gcggcttggt aatcctgaag ctggctgcga gtccaatggc cggcccgaaa
     2401 tacaccttcc actccggcca gatatcttcc tgtgtgactt agcacgctca ccgtgtcttg
     2461 agcatagcag cgcttgccgg cagccgactc aagtgcaaag ggagtagctt cccggccggg
     2521 gagaggaccg gatcggcaat ggccccactt cgaatacccc ggcggatccg atgacgcggc
     2581 gcttcgagtc cgtcggcgtc tctccccccg ggaacctcag cctgtcgcta acacccaggc
     2641 cgcgctcgag cgcggcggac gagaacaaga ccgcattcgc cacccgcttc cagcccgttg
     2701 tcgcgctacc atgaaatcta agtggcaatc ctggttggca ttcatgcggt tctggaagcg
     2761 ctgcgcgccg ggcgcagctt cgatcgcgta gtagtttcca agggcgccgg cggcgagcgc
     2821 attcaggagg tggtggatct gtgccgctcc gcctcggtcc ccacccgctt tgaaccgcgt
     2881 gcctcgctcg accggctggc cgggagttcc gcgcatcagg gcgtggtcgc tcttggcgcg
     2941 gcccaccgtt acgccaccat cgaggacatt ccctccggcg cgaaactgct ggtcgtgctg
     3001 gatggggtgg aggacccgca caacctggga gccgtcatcc gcaccgcgca cgccgccggg
     3061 gccgccgccc tggtgattcc ggagcggcgc gccgtgggaa tcaccgaggt ggtcgccaag
     3121 gccgcggccg gggcgctcga atacttgccg gtggtccgtg tcagcaacct caaccgtgca
     3181 ctcgaaactc tcaaggagag aggcttctgg atctacggct tcgacgagcg cggccagagc
     3241 atctacgacg agacggactt cgcctccccc accgttttcg tgttgggcgg cgagggccag
     3301 gggctgcatc atcaggttcg ggaacactgc gatctcgtga tgcgcatccc g
//
LOCUS       DATCGS010000243         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_366945, whole genome shotgun sequence.
ACCESSION   DATCGS010000243 DATCGS010000000
VERSION     DATCGS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366945"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="carA"
                     /locus_tag="VIL39_04965"
     CDS             complement(<1..1070)
                     /gene="carA"
                     /locus_tag="VIL39_04965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5480810.1"
                     /translation="MKAILALEDGSVFHGAGFGARASACGEVCFNTSMTGYQEILTDP
                     SYKGQIVTMTYPLIGNYGVNRDDVESWQPHVAGFVIRELSPIVSNWRAESSLSEYLEQ
                     NGIPGIQGIDTRALTRKLRVRGALNGLISTEGTAADEAVKRAKGWPGLIGVDYVKEVT
                     HKEPFLWDQKDEQSASFNLVRGPASAEAREAHDPLPPADIPIVAYDYGMKYNILRRLR
                     QHGFKVQVVPATTTAAEALQYKPAGIFLSNGPGDPAALGYAVQAVSDLVKAGLPIFGI
                     CLGHQILGQAFGGKTFKLKFGHRGGNQPVKELESGRVEITSQNHGFVVDPKTLPADVV
                     VDRINLNDQTVEGMRHKTKPVF"
     gene            complement(1092..>2444)
                     /locus_tag="VIL39_04970"
     CDS             complement(1092..>2444)
                     /locus_tag="VIL39_04970"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5480811.1"
                     /translation="DPANRFDDRADVLICQGRIVALGPDAAAKAPADVERMDVNGQVV
                     CPGLIDMHVHLREPGQVAKETIATGTATAARGGFTSVVCMPNTSPAIDNAGTVALIHE
                     RAAGQGVVNVYVAGAITKNLAGEELAPIGSLKKAGVVAITDDGHCVQNNDMMRRALEY
                     AKMFDLPVLDHCQDYSLVMDGVIREGYWSTVLGLRGWPAAGEEMIVARDILLAELTGA
                     QVHCQHISAAGSVKLLREAKKRGLPISGEACPHHFTLTDAAVAGSEKFLAADGNGIFG
                     FDGSRKGRPAWPAYDTNLKVNPPLGSARDREAVLEGLVDGTLEILCSDHAPHCDYEKD
                     VEFDYAPFGITGLETELALSLMQLYHTRRLGLADLIAKFTVAPARLLHLPKGTLSVGA
                     EADVTVFDPDCEWVFDRSRSASKSSNSPFYGWPLRGKVFATVVGGRKAWVEQREQAVA
                     "
BASE COUNT          455 a          859 c          663 g          467 t
ORIGIN      
        1 cagaaaaccg gcttggtctt atggcgcata ccctcgaccg tctgatcatt gaggttgatg
       61 cgatccacca ccacgtctgc cggcagcgtc ttcggatcca ctacaaaccc gtggttttgc
      121 gaggtaatct ccaccctgcc ggattcgagc tctttcaccg gctggttgcc gccacggtgc
      181 ccgaacttca gcttgaaggt cttcccacca aacgcctggc caagaatctg gtggcccagg
      241 catattccga aaatcggcaa gccggctttc actaagtcgc taaccgcctg caccgcgtac
      301 ccgagtgccg ccgggtcgcc cgggccgttc gagaggaaga ttccggctgg tttgtactgc
      361 agtgcctcgg ccgcagtagt agtcgccggc acaacctgaa ccttgaaccc gtgctgccgg
      421 agccggcgca ggatgttgta tttcatcccg taatcgtaag ccacaatggg aatgtccgcc
      481 ggcggcagcg gatcatgcgc ctcccgcgct tccgcagagg cggggccacg gaccagattg
      541 aaactcgcgc tctgctcatc tttctggtcc cacaagaatg gctctttgtg cgtgacctcc
      601 ttcacataat ccaccccaat caatcccggc catccttttg cccgcttcac cgcctcgtcg
      661 gcggcggtgc cttccgtgga aatgagaccg ttgagcgccc cgcgcacccg cagcttccgc
      721 gttaatgcgc gggtgtcgat tccctggatg ccgggaatgc cgttctgttc gaggtactcg
      781 gacagagaag actccgcacg ccagttgctc acgatcggcg acagctcgcg gatcacaaac
      841 cccgccacgt gcggctgcca ggattcgaca tcgtcccggt tcacaccgta gttgccgatg
      901 aggggatagg tcatcgtgac gatctgcccc ttgtaggacg ggtcagtcag aatctcctgg
      961 tagccggtca tcgaggtgtt gaagcaaacc tcgccgcagg ctgaggcgcg cgcgccaaaa
     1021 cctgcgccgt gaaacactga accgtcttcc agagccagaa tcgctttcat ctagaattcg
     1081 ccaaccagca gtcaggctac agcctgttcg cgctgttcaa cccatgcctt cctgcccccc
     1141 acgaccgtcg cgaacacctt gccgcgcaga ggccagccat agaaggggct gttcgaagac
     1201 ttgctcgcgc tcctgcttct gtcaaagacc cactcgcaat ccggatcaaa cactgtcacg
     1261 tcggcctccg cgccaacgct cagtgtccct ttcggcagat gcagcagccg ggcgggcgcg
     1321 acggtgaact tcgcgatcag gtccgccagg ccaagccgcc gggtatggta aagctgcatc
     1381 agcgacaggg ccagttccgt ttccaggccc gtgatgccga acggcgcata atcgaactcg
     1441 acgtccttct catagtcgca atgcggcgcg tggtcgctgc agagaatctc cagcgtgcca
     1501 tccaccagcc cctccagcac ggcctcgcga tcccgcgccg agcccagcgg cggattcacc
     1561 ttcaaattgg tgtcataagc cggccacgcc ggtcgcccct tacggctccc gtcaaaaccg
     1621 aatatgccgt tgccatctgc agccaggaat ttctcgctgc ccgccacggc cgcgtccgtc
     1681 aatgtaaaat gatgcgggca ggcctcgccg gaaatcggca gccctcgctt cttcgcctcg
     1741 cgcagcaatt tcacgctgcc cgctgcgcta atgtgctggc aatggacctg ggccccggtc
     1801 aactcggcca gcaggatgtc ccgcgccacg atcatttcct cacccgccgc cggccaccca
     1861 cgcaggccca acaccgtgct ccaatagcct tcgcggatta ccccatccat caccagcgag
     1921 taatcctggc aatgatccag caccggtaga tcgaacatct tcgcgtattc cagcgcacgc
     1981 cgcatcatgt cgttgttctg cacacaatga ccatcatcgg tgatggccac cacgcccgcc
     2041 ttcttgaggg agccgatcgg cgccagttct tcccccgcca ggttcttcgt gatggccccg
     2101 gcgacataga cattgaccac cccctgcccc gccgcgcgtt cgtgaatcag cgccaccgtc
     2161 ccggcattgt caatcgccgg cgacgtgttg ggcatgcaaa ccaccgaggt gaacccgccg
     2221 cgcgccgccg tcgccgtgcc ggtagcgatc gtctccttgg ccacctggcc cggctcacgc
     2281 agatggacgt gcatatcgat cagccctgga caaaccacct gcccgttgac gtccatccgc
     2341 tcgacgtcag cgggcgcttt ggccgcggcg tccggcccga gcgcgacaat tctaccctgg
     2401 caaatcagca cgtcggcccg gtcatcaaag cggttggcag ggtc
//
LOCUS       DATCGS010000244         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_517379, whole genome shotgun sequence.
ACCESSION   DATCGS010000244 DATCGS010000000
VERSION     DATCGS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517379"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            501..1640
                     /locus_tag="VIL39_04975"
     CDS             501..1640
                     /locus_tag="VIL39_04975"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY5480812.1"
                     /translation="MITPRGRGVFREQAREVLLAMRTVLGKCPEPMAITVQTVFLKDA
                     CDQAECKQLFVEFLGSNLPITNYVLQPPCCGAALALEAWAIGGKSVRVEHFGSHALAV
                     AYDGVRWVYCAGLEPAVSLSGVHPQTLDALGRLRAALRQAGSSYEHVVRTWFYLGDIT
                     EPEAGDQRYQELNRARTDFYRDIRFHCSSLASNPAQSVYPASTAIGMAGCGLVASCLA
                     LETQRDDVFLLPLENPQQTPAYAYHAKYSPKSPKFSRAVALGLGDYVTTWVSGTASIV
                     NSESCHLGDPAKQTEQTINNIERLISAENFARHGLKGAGTSLHDLAMVRVYVKRPEDL
                     AKCQAVCERRLGAVPAIYVVAGVCRPELLVEIEGVAFSRYAQAAA"
     gene            complement(1684..>2046)
                     /locus_tag="VIL39_04980"
     CDS             complement(1684..>2046)
                     /locus_tag="VIL39_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5480813.1"
                     /translation="GDAMAHALTLVDGLGVIGGGISGASPLFLPALVGELNSTYTSPK
                     GNKFRRLASVAFNLEDPAELKTFLKGETRTITVPGSKKKVQYDPLQRIGVGMSRLGTS
                     EAVAIGAYAFALRKLDER"
BASE COUNT          375 a          602 c          655 g          414 t
ORIGIN      
        1 ttatcgtgcc gtggacgctg gttcccaacg ttgaaacgtt gggctattat cgtatgtccc
       61 tacgggacaa agacttggcc ccgttttgtg ggtcttctct gggagtaaat cctagtggca
      121 ttggacaagc tgtccccgcc cctttcagca gggccttcga actggggctt gatgcagctc
      181 tgggcaagcg tcgggctgtc ccgggtaggc gaaaaagcaa gaagaggtat ggagaagcca
      241 ataaatatga aacttaccgc cggcaaaatc cgtctggaaa gatagtcagt tgcttgagat
      301 gggcagccat cctgctacaa ctaattgtca tgtgtgcact tttatcggct gcgcaaaggc
      361 gcgaacaggt tgcgaaaccc tcgcacagcc gcaacctgcc tttgcggtca cgcatggtca
      421 gggctgaaac cgggtccgga ctggcggaag gtgccgggtg ctcccacgtg gagctaggtg
      481 attccacgcg agttgccctc atgattactc cccgggggcg gggtgtgttt cgcgagcagg
      541 cccgggaagt gttattggcc atgcgcaccg tcctgggcaa gtgcccggag ccgatggcga
      601 tcacggtcca gacggtattc ctgaaagacg cctgcgacca agccgaatgc aagcagcttt
      661 tcgtggagtt cttgggctcg aacctgccga taaccaacta tgtgctgcaa ccgccctgct
      721 gcggcgctgc tttggccctg gaagcctggg ccattggtgg gaagtctgtg cgggtcgagc
      781 atttcggttc ccacgccctt gcggttgcct atgacggggt gcggtgggtt tattgcgccg
      841 gcctcgagcc tgccgtctca ttgagcggcg ttcatcctca aaccctggat gcgctgggac
      901 gtttgcgtgc cgcgctgagg caggccggta gcagttacga gcacgtcgtc cgcacctggt
      961 tctacttggg cgacatcact gagccggagg ctggggacca acgctatcag gaactgaacc
     1021 gcgcccgaac ggatttctac cgcgatattc gcttccactg ttcgtcgttg gcctcgaacc
     1081 cggcgcagag cgtctatccg gccagcaccg ccatcggcat ggccggctgc gggttggtcg
     1141 cgagttgcct ggcgctggaa acgcagcgcg atgatgtctt cctgctgccg ttggagaatc
     1201 ctcagcaaac tccggcttac gcctatcacg cgaagtattc gcccaagagc ccgaagtttt
     1261 cgcgcgcggt ggcgcttggg ctcggggact atgtgaccac ctgggtgtcg ggcaccgcca
     1321 gcatcgtcaa ttcggaaagc tgccacctcg gcgaccctgc aaagcaaacc gagcagacca
     1381 tcaacaacat cgagcggcta atctcggctg agaacttcgc tcgacacggc ctgaaaggcg
     1441 cgggcaccag cctgcatgat ctggccatgg tgcgcgtgta cgtcaaacgg cccgaggacc
     1501 tggccaaatg ccaggcggtc tgcgagcggc ggcttggcgc ggtgcccgcg atttatgtgg
     1561 tcgcaggagt gtgccggccg gaactactgg tcgagatcga gggcgttgcc ttctccagat
     1621 acgcgcaggc ggcagcgtag cagtccatcc aaagggaatc cgcagcccga tgaactttgc
     1681 ggcttatcgc tcgtccaact tccgcagcgc gaaggcgtag gcgccgatgg ccacggcttc
     1741 gctggtgccg aggcgggaca tgccgacgcc gatgcgttgc aacgggtcgt attgcacctt
     1801 cttcttgctg ccgggcacgg tgatcgtgcg cgtctcacct ttgaggaagg tcttcagttc
     1861 cgccgggtct tccaggttga aggcgaccga ggccagccgg cggaacttgt tgcccttcgg
     1921 gctggtgtag gtgctgttca actcgccaac cagcgccggc aggaaaaggg gcgaagcgcc
     1981 ggagatgccg ccgccaatga ctcctaggcc atccaccagt gtgagcgcat gggccatggc
     2041 gtcgcc
//
LOCUS       DATCGS010000245         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_468265, whole genome shotgun sequence.
ACCESSION   DATCGS010000245 DATCGS010000000
VERSION     DATCGS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468265"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..737)
                     /locus_tag="VIL39_04985"
     CDS             complement(63..737)
                     /locus_tag="VIL39_04985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5480814.1"
                     /translation="MGTSISFKRLDGKDTNGYLANAARGNAPGVVVIQEWWGLQDQIK
                     GMCDRFALAGFDALAPDLYKGKVVPYHDTDAAGKEMNSLDFMDLTTQTVRGAAQYLGR
                     NGAKVGLTGFCLGGAVTIIGATRIPELTAGVVFYGIPPEQAAKPADVKIPLQAHFANK
                     DDWCTPAAVDAFEKAMKAAGKSLELFRYDAEHGFANEQRMTVHDRQAAELAWGRATEF
                     FKKHLG"
     gene            complement(1024..>2673)
                     /locus_tag="VIL39_04990"
     CDS             complement(1024..>2673)
                     /locus_tag="VIL39_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY5480815.1"
                     /translation="TGFMGYVLPWGQMSFWGATVITNLFSAVPYFGESIVTLLWGGYS
                     VGNPMLNRFFSLHYLLPFLITGVVVLHVWALHVSGQNNPAGVEPKTEKDTVPFTPYAT
                     IKDAFGLCCFMLFFAWFIFYMPNYLGDADNYIPANPGVTPAHIVPEWYYLPFYAILRS
                     IPNKLAGVTAMFAAILVLAFLPWLDTAKTKSSRYRPLAKQFFWIFVVVCVLLGYLGAQ
                     PPEGIYVIAGRILTFCYFAYFLILLPLLSRIEKPRPIPNSIADDVLAKTAGRTAPVVS
                     TVIALMVAGALFAGSTQNARAEEQETPPSQKWSFAGPVGKFDRGSLQRGLKVYKEVCS
                     ACHALSYIAFRNLADPGGPGYSEAQASAFAAEYKIKDGPNDQGEMFDRPGRPADYFPS
                     PFPNEQAARAANGGALPPDLSLITKARSYERVFPKFVFDFFTQFQEQGPNYVDALLQG
                     FEEKPPADVTLPEGSYYNKYFPGHAIKMPKPLSDGQVTFDDGSPGTVAQYAKDVTTFL
                     AWTAEPQMEARKKMGMQVFVFLIILTLLLYFTKKKVWADAH"
BASE COUNT          495 a          817 c          862 g          499 t
ORIGIN      
        1 tgccgcggag tatgggtccc ggcgttcgcc gggacgacgg catagagttt tgccaactct
       61 tctcatccca gatgcttttt gaaaaactcc gtggcgcggc cccaggccag ctccgcggcc
      121 tggcgatcat gcaccgtcat ccgctgctcg ttggcgaaac cgtgctcggc gtcatagcgg
      181 aacagttcga gcgatttgcc ggcggccttc atcgccttct cgaaggcatc gaccgctgcc
      241 ggcgtgcacc agtcgtcctt gttggcgaaa tgcgcctgca gcggaatttt cacgtccgcg
      301 ggcttggccg cctgctccgg cggaatgccg tagaacacca cgcccgcggt caattcggga
      361 atcctggtgg cgccgatgat ggtgaccgcg ccgccgaggc agaagccggt caggccgacc
      421 ttggcgccgt tgcggccgag atattgcgcg gcgccccgca cggtctgcgt cgtgaggtcc
      481 atgaaatcca gcgagttcat ttctttgccg gcggcgtcgg tgtcgtgata gggcaccacc
      541 ttgcccttgt agagatcggg cgccagcgca tcgaagccgg ccagcgcgaa gcggtcgcac
      601 attcccttga tctggtcctg cagcccccac cattcctgaa tcacgaccac gcccggcgcg
      661 ttgccgcgcg cggcgttggc gagataaccg ttggtgtcct tgccgtccag gcgcttgaaa
      721 gagatgctgg tacccatggg ttcctccggt gagctttttg ggatggccga tttgcggcca
      781 ttttgtccgc tgtgcggtca caacgcaatc ggttggtcgc ggagttcgtg cccctgaggc
      841 tgcgcagcgc gccgctcttg cggcgtggtg cgctgctgat ccggagccca ttgagtggga
      901 ggcgctcgtg ggtcccggct ctgcgaagcg gtgctgcgca ccgcatcgcg tccgggacac
      961 gcacccagcc caacaaaaag cccccgcggg ggctttctcg aattcacatc atcgctgcca
     1021 agatcaatgc gcgtcggccc agaccttctt cttggtaaag tacagcagca gcgtcaggat
     1081 gatcagaaac acgaacacct gcatccccat cttcttgcgc gcttccatct gcggctccgc
     1141 ggtccacgcc aggaaggtag ttacatcctt ggcgtattgc gccaccgtcc ctggcgaccc
     1201 atcgtcaaac gtgacctggc cgtcgctcag cggcttcggc atcttgatgg cgtggccggg
     1261 gaaatattta ttgtagtagg agccctcggg aagggtgacg tcggccggcg gcttttcttc
     1321 aaagccctgc agcagcgcgt cgacgtagtt cggaccctgc tcctgaaatt gcgtgaagaa
     1381 gtcgaacacg aatttcggga agacgcgctc ataggagcgt gccttggtga tcagggacag
     1441 gtcgggaggc aatgcgccgc cattggcagc gcgggcggcc tgctcgttgg ggaacggcga
     1501 ggggaagtaa tcggcgggcc ggccgggccg gtcgaacatt tcgccctgat cgttggggcc
     1561 gtccttgatc ttgtattcgg cggcgaaggc cgaagcctgc gcttccgaat atccgggacc
     1621 gccgggatcg gcgagattgc ggaacgcgat atatgacagc gcgtggcagg cggagcagac
     1681 ttccttgtag accttcagcc cgcgctgcaa cgagccgcga tcgaacttgc cgacggggcc
     1741 cgcgaacgac catttctgcg acggcggcgt ttcctgctcc tcggcgcggg cattttgcgt
     1801 gctgccggcg aacagcgcgc cggcgaccat cagcgcgatc actgtggaca ccaccggcgc
     1861 cgtcctgcct gcggtcttcg ccagcacgtc gtcggcgatc gaattcggaa ttggcctcgg
     1921 cttttcgatc cggctcaaca gcggcaacag gatcaggaaa taggcgaagt agcagaaggt
     1981 caggatgcgc ccggcaatca cgtagatgcc ttcaggcggt tgcgcaccga gatagcccag
     2041 cagcacgcag acgacgacga agatccagaa gaactgcttt gccagcggac ggtacctgga
     2101 cgatttcgtt ttcgcggtat cgagccacgg caggaacgcc agcaccagga tcgccgcgaa
     2161 catcgcggtc acgccggcga gcttgttcgg gatgctgcgc aggatcgcgt agaacggcag
     2221 ataataccac tccggcacga tatgcgccgg ggtcacgccg ggattggcgg ggatgtagtt
     2281 gtcggcgtcg ccgagatagt tcggcatata gaagatgaac cacgcgaaga acagcatgaa
     2341 gcagcagagg ccgaacgcat ccttgatggt cgcgtagggc gtgaacggca cggtgtcctt
     2401 ttccgtcttg ggctctacgc cggccggatt gttctgaccc gatacatgca gcgcccacac
     2461 gtgcagcacc acgacaccgg tgatcaggaa cggcagcaga tagtgcagcg agaagaagcg
     2521 gttcagcatc ggattgccga cggaatagcc gccccacagc aaggtcacga tgctctcgcc
     2581 gaaataggga acggcggaga acaggttggt gatgacggtg gcgccccaga agctcatctg
     2641 gccccatggc agcacgtaac ccatgaagcc ggt
//
LOCUS       DATCGS010000246         5763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_367256, whole genome shotgun sequence.
ACCESSION   DATCGS010000246 DATCGS010000000
VERSION     DATCGS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367256"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VIL39_04995"
     CDS             <1..467
                     /locus_tag="VIL39_04995"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEY5480816.1"
                     /translation="LTLAVPPDEPTVPRKVIVWTVVTVVILVLGLIVVVAGLNHYAKL
                     AERQRARTGAVAGTKDTATAPGFEVSTFSLEKGQGSDGSYAVGTVVNTYNRPRSRVAL
                     KLDLLDAGGQKVSIAKAYRPVLEPRAKWEIKLPVAGDSKAVSARVASIQEGQ"
     gene            complement(478..1686)
                     /locus_tag="VIL39_05000"
     CDS             complement(478..1686)
                     /locus_tag="VIL39_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5480817.1"
                     /translation="MTINHQLINWLSQVASVSKFGLLSIPQRGGSVVAAVIGIAGVVA
                     VLVGVLSMGVGFQKAMTASGSPDAAIVLRSGADTEMVSGFLRESTRIIADAPGVARTE
                     DGALASAELFVIISLPKRSTGTDANVPMRGVEHAAVKVRDKLKLVDGRMFEWGKNEVV
                     VGVGAANEFAGLELGDKLRVGRRDWPIVGVFSAGGGTAESEVWTDASVLQAAYKRGDT
                     FQSVYVKLVSPGAFQEFKDALTTDPRLNVKVLRQSEYYAEQSTAVTRLITTLGLLIAF
                     LMAIGAVFGALNTMYSSVSARTREIATLRALGFGSGAVVVSVMIESLALGVLGGSLGA
                     TLAYFTFNGLQTATMNWQSFSQVTFAFAVTPDLLVRGIVWAVLIGLIGGLFPALRGAR
                     LPIATALREP"
     gene            complement(1707..2861)
                     /locus_tag="VIL39_05005"
     CDS             complement(1707..2861)
                     /locus_tag="VIL39_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5480818.1"
                     /translation="MKYLPLIWSNLKRKKLRTLLTLLSIVVAFILFGFLSAIRQALAG
                     GVAMAGQNRLVVRHKISIIQSLPQSYKARMERIPGVAAATHESWFGGVYQDTKNFFPQ
                     MPVEPEEFLDMYPEFLLPPEQKQAWLETRTGAIVGRRTAERFHWKIGDRVPIQSSIWG
                     KTDGSRLWEFDIVGIFDGRDKATDTTPLYFQYDYFEEARAWNKGQVGWYLVRVKDPAQ
                     AAEVAKLVDQEFENSAAETKTEPEGAFLQAWAKQIGDITLITATILSAVFFTILLVAG
                     NTMGQAVRERTGELGALKAMGFTSGQVLSLVLAESCVLAVLGGGIGLSLAWLTIARGD
                     PTGGLLPMFYFPTVDVLIGVGLSVALGVFAGILPALQAMRLRVADALRRM"
     gene            complement(2867..3628)
                     /gene="rph"
                     /locus_tag="VIL39_05010"
     CDS             complement(2867..3628)
                     /gene="rph"
                     /locus_tag="VIL39_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY5480819.1"
                     /translation="MAQPQSLSISPAPRADGRGPDQLRAVRFQNHIAPYATGSTLIEW
                     GNTRVICGVTVEESVPRWMKEQGVTGGWITAEYSMLPYSTLQRKQRDITKGKIDGRSQ
                     EIQRLIGRAMRAAIDLDKLGSRTIWVDCDVLQADGGTRTAAITGAYVAMALATRKLVA
                     EGKLSADPMRHAVAAVSVGIVGKQPLLDLCYTEDAAAEVDLNMAMTAAGEFIELQGTG
                     EEATFSQSQLADMLALGRFGIGELLALQQAALAAQ"
     gene            3840..4136
                     /locus_tag="VIL39_05015"
     CDS             3840..4136
                     /locus_tag="VIL39_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480820.1"
                     /translation="MKTKLFTTVLFVLALLLGTVNPAFAAGDTGATSVAIDVIVARPV
                     SLAMTIIGSALFVVSLPVAIPAQSVEKVAHTLVVTPAKDTFTRPLGDFDSFLDY"
     gene            complement(4171..5112)
                     /locus_tag="VIL39_05020"
     CDS             complement(4171..5112)
                     /locus_tag="VIL39_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY5480821.1"
                     /translation="MTNSLPRPEVIITHESDLDGLVSGVLLQRLARKLFNADVRLEAY
                     HYHNWRQRDLREKCGWIADLTFEQRMDRPNWVIIDHHITETPPKNASFIYDPKKSAGL
                     ICYELCQQQGLGSPELDRLVHLNNVADLFLEDDPDFILATDYANLVKTYQFWNLHALI
                     SGQIEQLLNNPLLRVMEVKRNIENPLGFAWSRENVSELTPQVGFVDTIIGNNNLIVHQ
                     LLEQQATKYPVLLTLFRRANGVVIASLRSRNGEALKIAEKLQGGGHPNASAATLPRSI
                     KNIPDAVIYLRQTLNPKADEPLNSLEDIFASIDLSQK"
     gene            5286..>5763
                     /locus_tag="VIL39_05025"
     CDS             5286..>5763
                     /locus_tag="VIL39_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5480822.1"
                     /translation="MTIPFQVNLKDQVAVVTGGGGILCSVMADAVAQCGAKVAILDLK
                     PEMAQKAADQITKAGGIAKAFACNVLEKASIERAHEQVKAAFGPVDILINGAGGNHPK
                     GTTSKEYLSREDLTAKKEGVVTFYDLTVEGIQFVFNLNFLGTFLPTQVFSKDMVQKG"
BASE COUNT         1095 a         1744 c         1857 g         1067 t
ORIGIN      
        1 aattgaccct ggccgtcccg ccggacgaac cgactgtgcc tcggaaggtc atcgtctgga
       61 cggtcgtcac ggttgtgatt ctggttctgg ggctgatcgt ggtggtggcg gggctgaacc
      121 actatgcaaa gctggcggag cgtcaaagag cccgcacggg ggcggtagcc gggaccaaag
      181 ataccgcgac ggcgccgggc tttgaggttt cgaccttctc gctcgaaaag gggcagggca
      241 gtgacgggag ttatgccgtc ggcacggtgg tgaacaccta caatcgtccg cgctcgcgcg
      301 ttgccctgaa actcgacctg ctcgatgccg ggggacagaa agtgagcatc gccaaggctt
      361 atcggccggt gctcgagccc cgggcgaagt gggagatcaa gctgcccgtc gcgggcgatt
      421 ccaaagccgt ttccgcgagg gtggcttcca tccaggaagg gcagtagggc gggttcttca
      481 aggctcgcgc aacgcggtgg cgatgggcag ccgggcgccg cggagagcgg ggaagagacc
      541 gccaatcaga ccgatgagga cggcccacac aatcccgcgc acgagcaggt cgggcgttac
      601 ggcgaaggcg aaggtgacct ggctgaagct ttgccagttc atcgtggcgg tctggaggcc
      661 gttgaaggtg aaataggcaa gcgtggcccc gaggctgccg ccaagcacac ccagcgccag
      721 cgattcaatc atcaccgata cgacgaccgc gccgctgccg aacccgaggg cgcgcagggt
      781 ggcgatttcc cgcgttcgcg ccgagacgga gctgtacatc gtattgagcg cgccgaagac
      841 ggcgccgata gccatcagga acgcaatgag caagcccagc gtcgtgatca gccgcgtgac
      901 ggcagtggat tgctcggcgt aatactcact ctggcggagc accttcacgt tgaggcgggg
      961 gtcggtggtg agcgcgtcct tgaactcctg gaacgcgccg ggcgagacga gtttgacata
     1021 gacggactgg aaggtgtcgc cgcgcttata ggcggcctgt agaacgctcg cgtcggtcca
     1081 gacctcggat tcggcggtgc cgccgccggc ggagaagacg cccacgatcg gccagtcgcg
     1141 gcgcccgacg cgcagtttgt cgccgagctc aagcccggcg aattcattgg cagcgccgac
     1201 gccgacgacg acttcattct tgccccactc gaacatgcgg ccgtccacca gcttgagctt
     1261 gtcgcgcacc ttgaccgcgg cgtgctcgac gccgcgcatc ggcacattcg cgtcggtgcc
     1321 ggtggagcgc ttggggaggc taatgatgac aaacagttcc gcggaggcga gggcgccatc
     1381 ttcagtgcgg gcgacgcccg gggcatccgc gatgatgcgc gtgctctcgc gcaggaaacc
     1441 gcttaccatt tcggtgtcgg ccccgctccg cagcacgatg gccgcatcgg gcgacccgga
     1501 ggcggtcatg gccttctgga acccgacgcc catcgagagc acgcccacga gcacggcgac
     1561 caccccggca atgccgatga cggcggccac caccgacccg ccccgttgcg gaatgctcag
     1621 caaaccaaac ttgctgacgg aggcgacttg cgagagccag ttgattagtt gatggttgat
     1681 agtcaagagt tgatggggga caggaatcac atccgcctca gggcatcggc tacgcgcagg
     1741 cgcatggctt gcagggcggg gaggatgccg gcgaacaccc cgagcgccac gctcaacccg
     1801 acgccgatga gcacatcaac ggtcgggaag tagaacatcg gcaacaaacc gccggtgggg
     1861 tcgccgcgcg caatcgtcag ccaggccagg ctcaggccga tgccgccgcc cagcaccgcc
     1921 agcacgcagg attccgccaa caccagcgaa agcacctgcc cgctggtgaa acccattgcc
     1981 ttgagcgcgc ccagttcgcc ggtgcgctcg cgcacggcct ggcccatggt gttgcccgcg
     2041 accagcagga tcgtgaagaa cacggcgctc agaatcgtgg cggtgatcag cgtgatgtcg
     2101 ccgatctgct tggcccaggc ctggaggaag gcgccttctg gctcggtctt ggtctccgcc
     2161 gccgagttct cgaattcctg gtccaccagt ttcgcaacct ccgccgcctg cgccgggtct
     2221 ttcacgcgca cgaggtacca tcccacctgg cccttattcc acgcgcgagc ttcctcgaag
     2281 taatcatact gaaagtagag cggcgtggtg tcggtggcct tgtccctgcc gtcaaagatg
     2341 ccgacgatgt cgaattccca aaggcggctg ccgtcagtct ttccccaaat ggaggactgg
     2401 atgggcaccc ggtcaccgat cttccaatgg aatcgctcgg cggtcctgcg gccgacgata
     2461 gcccccgtgc gcgtttccag ccaggcttgc ttctgttccg gcgggaggag gaactcgggg
     2521 tacatgtcca ggaattcctc cggctcgacg ggcatttggg ggaagaagtt cttggtgtcc
     2581 tggtaaacgc cgccaaacca ggactcatgg gtggccgcgg ccacgccggg aatacgctcc
     2641 atgcgggcct tgtatgattg cggcagcgac tggatgatgg aaatcttgtg ccgcaccacc
     2701 aggcggttct ggccggccat ggccacgcct ccagcgaggg cctgcctgat ggccgagagg
     2761 aagccaaaga ggatgaaggc caccacgatc gagagcagcg tcagcagcgt gcggagtttc
     2821 ttccgcttca gattgctcca gatcaggggc aggtacttca ccggacctac tgagcggcga
     2881 gggccgcctg ctggagggcg agcaactcac ctatcccgaa tctgcccaaa gcgagcatat
     2941 ccgctagttg gctctggctg aaggtcgcct cctcgcccgt gccttgcagt tcgatgaact
     3001 cgcccgcggc ggtcattgcc atattcaagt ccacttcggc ggcggcgtct tcggtgtagc
     3061 aaaggtccag caagggctgc ttccccacga tgccgacgct gaccgccgcg acggcgtgcc
     3121 gcattgggtc ggccgacagc ttgccttcgg cgaccagctt gcgtgtcgcg agagccattg
     3181 ccacgtaggc gccggtgatc gcggcggtgc gcgtgccgcc gtcggcctgc aacacatcgc
     3241 agtccaccca gatcgtgcgg gagcccagct tgtccaggtc aatcgccgcg cgcatcgccc
     3301 ggccgatcaa gcgctggatt tcctgcgagc gcccgtcaat cttgcccttg gtgatgtcgc
     3361 gctgtttgcg ctgcagggtc gagtagggca gcatcgagta ttcggccgtg atccagccgc
     3421 cggtgacgcc ttgctccttc atccagcgcg gcacggattc ttcgaccgtc acgccacaaa
     3481 tgacgcgcgt atttccccat tcgatcagcg tcgagccggt ggcatagggc gcgatgtggt
     3541 tctggaagcg aacggcccgg agttgatccg gcccacggcc atcggcacgg ggggcaggac
     3601 tgatagatag tgattgaggc tgcgccataa aggtgcgtca tgttatgaca gtccgccccg
     3661 acggggcaac gacgaaatcg gcgatatgac gaaacaggcc cgcacttgac agcggtgcag
     3721 ggtattgtac gctgttttga gtgagcagga ccaattatca ctttcctgtg ttcgggtggg
     3781 cttgcagcgg caacttcccg tcaagagagc gttctgccgc caataaatgt ctaggccaaa
     3841 tgaaaaccaa gctatttact actgtcctgt ttgtgctggc gcttctactc ggcacggtaa
     3901 accccgcttt cgccgctgga gacacaggcg cgacgtcggt tgccattgat gtgattgtcg
     3961 cgcgcccggt ctcactcgcc atgaccataa tagggtcggc gctgttcgtg gtcagcctgc
     4021 ctgtggctat cccggcccaa agcgtcgaaa aggtcgccca cacgttagtg gtcactccgg
     4081 ccaaggacac gttcacccgg ccgctgggcg actttgatag cttcctggac tactgaccgc
     4141 ttcagcggct gccgcgacct gtcgagccga tcacttctgc gacaagtcaa tggaggcaaa
     4201 gatgtcttcc agactgttca agggctcatc cgctttcggg ttgagggtct ggcgcaggta
     4261 gatgacggca tcgggaatgt tcttgatcga gcggggcagg gtggccgcgc tggcgttggg
     4321 atggccgccg ccctggagct tttcggcgat ctttagcgcc tcaccgttgc ggctgcgcag
     4381 gctggcgata accacgccgt tcgcgcggcg gaagagggtc agcagcacgg gatacttggt
     4441 cgcttgctgt tccaggagtt ggtggacgat caggttgttg ttgccgatga tcgtatccac
     4501 aaagcccacc tgtggggtga gttcgctgac attctccctg ctccacgcga agccgagcgg
     4561 gttctcgatg ttgcgtttga cttccatcac cctaagcagc ggattgttca ggagctgctc
     4621 aatctggccg ctaataagcg catgcaggtt ccagaactgg taggtcttga ccaggttcgc
     4681 gtagtcggtg gccaggatga agtcggggtc gtcttcgaga aacaggtcgg cgacgttgtt
     4741 caaatgcaca agccggtcca gttcgggcga cccaaggccc tgttgctggc aaagctcata
     4801 gcagatcagg ccggcagatt tcttcgggtc atagatgaac gacgcgttct tgggtggggt
     4861 ctcggtaatg tggtggtcaa tgatgaccca gtttggccgg tccatgcgct gctcaaaggt
     4921 caggtcagcg atccagccgc atttctcccg caggtcgcgc tgccgccaat tgtggtaatg
     4981 ataggcctcc agccgaacgt cggcgttgaa cagttttcgc gccagccgct ggagcaagac
     5041 accggacacc agcccgtcga ggtcgctctc gtgggtgata atcacttccg gtctaggcaa
     5101 ggaattcgtc attggacaga cattaagcga agccggcttg gctggcgagt ggggaattca
     5161 ctccggccta tgtcaggaaa atactggcgc gccctggagt ttccagtatt gttcacgcgg
     5221 cgttgacgac tcggcccaca cggcttgagc ccggcgccga caaacaattt aaggcacagc
     5281 aaactatgac tataccattc caagtgaatc tgaaagacca ggtggccgtt gttaccggcg
     5341 ggggcggcat cctgtgcagt gtgatggccg acgcggtcgc ccaatgcggc gccaaggtcg
     5401 ctatcctgga cctcaaacct gagatggccc aaaaggccgc cgaccaaatt accaaagccg
     5461 ggggcatcgc caaggccttc gcctgcaacg tgctggagaa ggcaagcatc gagcgggcgc
     5521 acgaacaagt caaagccgcg ttcgggccgg tggacattct catcaacggc gccggtggca
     5581 accatccgaa gggcacgact tcgaaagagt acctctcccg cgaagacctc acggccaaga
     5641 aagagggcgt ggtgacgttc tacgacctga cggtcgaggg catccagttc gtgttcaacc
     5701 tgaacttcct gggcaccttc ctgccgacgc aggttttcag caaggacatg gtgcagaagg
     5761 ggc
//
LOCUS       DATCGS010000247         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_71708, whole genome shotgun sequence.
ACCESSION   DATCGS010000247 DATCGS010000000
VERSION     DATCGS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71708"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VIL39_05030"
     CDS             <1..820
                     /locus_tag="VIL39_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5480823.1"
                     /translation="VLVSAGGGDNMMGAIGTGNTRAGVITASFGTSGTIYACSEKPVV
                     DPQGEIAAFCDSTNRWLPLLCTMNVTVATEMVREDFGWSHEKFAAEAARIPAGSRGLL
                     LLPYFEGERTPNVPDGTGVWFGVNQKTFEAGHFARAAMEGVTLGMNYGLRRLAELGVK
                     PAQIRATGGGAKSKVWRQIMADIFDAEVVTLKVSEGAAYGAALQALWCWRLQQGEKVS
                     IGDLTDQFVGLNADETAEPDSKNAAIYRELQGIQDEMSVALRGAFKRHRQFLLR"
     gene            complement(838..>2098)
                     /locus_tag="VIL39_05035"
     CDS             complement(838..>2098)
                     /locus_tag="VIL39_05035"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY5480824.1"
                     /translation="GSELMLGRVLNTHQQWLCRQLADLGYVVSRQVAVPDTGSDIEQA
                     VREALARADLVIATGGLGPTSDDLTRDLVAQMLGKRLREDAAVLAHISHFFESRKRPM
                     PERTRVQAQVPEGATVLPNPHGTAPGLAMEVRPNPFRAGGRPSWLIMLPGPPRELRPM
                     FSDTVVPLLGRVLPLAGRFVCRTLRTTGVGESVVEEKISGPLQRWVEAGLDLGYCARP
                     GQVDVRLAARGGEAEQQVREAEMVVRELLGPHIYGVEDEDLETAVVRLLTERKATLAL
                     AESCTGGCIANRVTNVPGASVVLLAGLVTYSNAAKQKFLGVRADTLAQQGAVSEAVAR
                     EMAEGARRQTQADYALSVTGIAGPSGGTPGKPVGTVFIGLAGPSGTRVEGQFNPYDRE
                     TFKQVTAQQALELLRRTMLSGGVENSA"
BASE COUNT          345 a          694 c          688 g          371 t
ORIGIN      
        1 cgtgctggtg agtgccggtg gcggagacaa catgatgggc gccatcggca ccggcaacac
       61 gcgcgcgggc gtcatcaccg ccagcttcgg caccagcggc accatctatg cctgctcgga
      121 gaagccggtc gttgacccgc aaggcgagat cgccgctttc tgtgattcga ccaaccgctg
      181 gttgccgctc ctctgcacca tgaatgtgac cgtcgccacg gagatggtgc gcgaagattt
      241 cggctggtcg catgagaagt tcgccgccga ggcggcgcgc attccggcgg gctcgcgggg
      301 cctgctgttg ctgccgtatt ttgagggcga gcgtacgccg aacgtgccgg acgggacggg
      361 tgtctggttc ggcgtgaacc agaagacctt cgaggccggc cactttgccc gcgcggcgat
      421 ggagggcgtg acgttgggca tgaactacgg gttgcgccga ttggcggaac tgggtgtcaa
      481 accggcgcaa atccgcgcca cgggcggcgg cgccaagtcc aaggtctggc ggcagatcat
      541 ggcggatatc ttcgacgcgg aagtggtcac cttgaaagtc agcgagggcg cggcttacgg
      601 cgcggcgttg caggcgctct ggtgctggcg cttgcagcaa ggcgaaaagg tttccatcgg
      661 cgacctcacc gatcagttcg tgggactgaa tgccgacgag acggcggaac cggattccaa
      721 gaacgctgcc atctatcgcg agttgcaggg catccaggac gaaatgtccg tggccctgcg
      781 cggcgcgttt aagcggcacc ggcagttcct gttgcggtaa tggcttccgg ctgtcggcta
      841 cgccgagttt tccacgccgc ctgagagcat tgtccgccgc aacaactcca aggcttgctg
      901 ggccgtgacc tgcttgaagg tctcgcgatc gtaaggattg aattggcctt ctacccgggt
      961 gccggacggg ccggctaatc ctatgaacac ggtcccgact ggcttgccgg gcgttccgcc
     1021 gctgggaccg gcaatccccg ttaccgacaa agcgtaatcc gcctgggttt gccggcgggc
     1081 gccttcggcc atttcgcgcg ccacggcttc gcttaccgcg ccttgctggg ccagagtgtc
     1141 cgctcggacg ccgaggaact tctgcttcgc tgcgttgctg taagtgacca gtccggcgag
     1201 caggaccacc gacgcgccgg gcacattggt cacccgattc gcgatacagc ctccagtgca
     1261 ggactcggcg agtgcgagcg tcgctttgcg ttcggtcaat aggcggacaa cggccgtctc
     1321 caggtcttcg tcttccacac cgtagatgtg cggaccgagc agctcccgaa cgaccatctc
     1381 ggcttcccgc acctgttgtt ccgcctcgcc tccgcgagcc gccaggcgca catcaacctg
     1441 gccgggccgc gcgcagtagc cgaggtcgag tccggcctca acccagcgct gcaatgggcc
     1501 gctgatcttc tcctcgacca ccgactcgcc aacgcccgtc gtcctcaagg tgcgacacac
     1561 aaacctgccg gccagcggca acactcgccc aagcagcggg acgaccgtgt cgctgaacat
     1621 cggccgcaac tcgcgcggcg ggccgggcag cataatgagc cagcttgggc ggcccccggc
     1681 acgaaatggg ttgggccgga cttccatcgc caatccggga gcggtgccgt gcgggttggg
     1741 cagaaccgtt gccccctccg gaacctgggc ctgcacccgc gttcgttccg gcatgggccg
     1801 cttgcgactc tcgaagaaat ggctgatatg cgccagcacc gccgcgtcct cgcgcaacct
     1861 tttcccgagc atctgcgcca ccaggtcccg cgtcaggtcg tccgaagtgg ggcccaggcc
     1921 gccggtcgcg atcacgagat cggcgcgtgc gagcgcctcc cgaacggctt gttcaatgtc
     1981 actgcccgtg tccggcactg cgacctggcg gctgacaaca tatcccaggt ccgcgagctg
     2041 tcggcagagc cattgctggt gggtattgag cacgcgcccg agcatcagct cgctgccg
//
LOCUS       DATCGS010000248         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_71897, whole genome shotgun sequence.
ACCESSION   DATCGS010000248 DATCGS010000000
VERSION     DATCGS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71897"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VIL39_05040"
     CDS             <1..948
                     /locus_tag="VIL39_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5480825.1"
                     /translation="HRWLMFLLVVLPVVTALAVGGGSLLVKRALLPVDKIAASAERIS
                     SQNLSERLPVARTGDELERLSIALNHMIQRLDEAFQYSRQFMADASHELRTPLTVLRG
                     ELESVIQEATLAPEWRERLGSALEEVERLASIVEGLFAISRLDAGEAAAEWVKLDLAR
                     LTAATADQMSLLAEDKNIQVTCVAAESVWVEGDRARLKQVVVNLLDNAIKYTLQDGAI
                     ALTVSASNSRAVLEVADNGIGIPAEALPRVFDRFFRVDKARSRELGGAGLGLSIVKSI
                     CTAHHGRVEASSLPGQGSRFRVELPLAKPSTNNEKKHNS"
     gene            945..2192
                     /locus_tag="VIL39_05045"
     CDS             945..2192
                     /locus_tag="VIL39_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5480826.1"
                     /translation="MSTESLESQQPAGSPVEAQPAKPLRPLTGRLAGCLRHIDWRIIG
                     IMFLAAASIPVWRAVSVHAQPQMATGSAAPPLTVAVARVTREDLYNEVTIPAEFRPYL
                     KVELHAKVSGYVDQINVDIGDQVKAGQLLARLEVPELRDELARAKAAEQRAGADYKDA
                     HLVYTRLLAVDKAHPNLVAQQELDAAEAKDNTSEAAIAGAKAEAQRQQTLLAYTHITA
                     PFDGVITHRYADPGSLIQAGTASDTQSMPLVCLSDNYRLRLDFPVSVAYVKDIQIGDR
                     VEVRVQSLKGKPFTGTISRSTQKVDEDTRTMIMEIEVPNPKLELVPGMYATVVLQVDR
                     RPQVLAIRTEAISTDKKTLVYVVNREQEIEERPVTLGLETPGKYEVVDGLKEGDLVMI
                     GSRSQVKPGQKVEAKLIDSQAQQ"
     gene            2189..>2732
                     /locus_tag="VIL39_05050"
     CDS             2189..>2732
                     /locus_tag="VIL39_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY5480827.1"
                     /translation="MNTPLTKELLESLRRLDGCTLANAIETFNVRLRNEGFTDGGLRC
                     FFPLLPPIVGYAATVKIRGSAPPTAGGYYPQRTDWWEYVRSLPAPHIVVAQDIASHRG
                     LGAMLGQVHFNIQRALGAVGIVTNGAVRSIPTAETLGFQVFAAGVSVSHAYTHLLEFG
                     TPVEVDGLKVHSGELLHGDQH"
BASE COUNT          538 a          824 c          872 g          498 t
ORIGIN      
        1 caccggtggc ttatgttcct cctcgtagtg ctcccggtcg tgacggcgct ggcggtgggc
       61 ggcggctccc tgctagtgaa acgagcgctc ttgccggtgg acaagattgc cgccagcgcg
      121 gagcggatta gttcgcagaa cctcagcgag cgcctcccgg tcgcccggac gggcgatgag
      181 ttggagcgcc tgtctattgc gctcaaccac atgatccaac gcctggatga agccttccaa
      241 tactcgcgac agttcatggc tgatgcatcg cacgagctgc gcacgcccct gaccgtgctg
      301 cggggcgaac tcgaaagtgt catccaggaa gcaaccttgg cgcccgagtg gcgcgagcgg
      361 ctgggcagcg cgctggagga agtcgagcgc ctggccagta tcgtcgaggg gctgtttgcc
      421 atctcccggc tcgatgccgg ggaggccgct gcggaatggg tgaaactcga tctggcccgc
      481 ttgaccgcag cgaccgcgga ccagatgtct ctgctggccg aggacaagaa cattcaggtc
      541 acatgcgtcg ccgccgaatc ggtatgggtg gaaggcgacc gggcgcgctt aaagcaggtg
      601 gtggtgaacc tgctcgacaa tgcgatcaaa tacactctcc aagacggcgc cattgctctg
      661 accgtcagcg ccagcaacag ccgcgcggtg ctggaggtgg cggacaatgg catcgggatt
      721 ccggcggaag cgctgccgcg ggttttcgac cggttctttc gcgttgataa ggcgcgttcg
      781 cgcgaactcg gcggcgcggg cctgggcttg tcgatcgtca aatccatttg cacagcccac
      841 cacgggcgcg tcgaggctag cagcctcccg ggccagggca gccggttccg ggtggaattg
      901 ccgctggcca agccgtcaac gaacaacgaa aagaagcata actcatgagc actgaatcgc
      961 tggaatctca acaacctgcc ggcagccccg ttgaagcgca gccggcaaaa cctctgcggc
     1021 ccttgactgg ccggctagca ggctgccttc ggcacattga ttggcggatc atcggaatca
     1081 tgtttctcgc tgcggcttcg attccggttt ggcgcgcagt cagcgtccac gcacagccgc
     1141 agatggcgac gggctcggca gcccctccgc tgacagtcgc cgttgcgcga gtgactcgcg
     1201 aggaccttta caatgaagta actatccctg cggagttccg gccatatctc aaagttgagt
     1261 tgcatgcaaa ggtctcgggg tatgtagatc agatcaacgt tgatattggc gaccaggtca
     1321 aggccggcca gcttttggcc cggctcgaag tgccggaatt gagagacgag ctggcgcggg
     1381 cgaaagcggc ggaacagcga gcgggggctg attacaagga tgcccatctg gtttacaccc
     1441 gcctcttggc ggtggataag gcacatccca atctggtggc ccagcaggaa ctggatgctg
     1501 ccgaggcaaa ggacaacacc tcggaggcgg ccatcgccgg ggccaaggcg gaggcgcaaa
     1561 ggcagcaaac gctcctggca tacacgcaca tcacagcgcc gtttgacggg gtgattaccc
     1621 atcgttacgc ggacccgggc tcgctcatcc aggcggggac cgcttccgac acccaatcca
     1681 tgcccttggt gtgcctctcg gataattacc gcctccggct cgatttcccg gtttccgtcg
     1741 cctacgtcaa agacattcag atcggtgacc gggtggaggt gcgtgtgcaa tctctcaagg
     1801 ggaagccgtt cacgggtact atctcacgtt ccacgcaaaa ggtggacgag gacacccgga
     1861 cgatgattat ggaaatcgag gtgcccaacc caaagctgga attggtgccg ggcatgtatg
     1921 ccaccgtcgt tttgcaggtg gaccggcgac cgcaggtgct ggccattcgc acagaggcga
     1981 tttccacgga caagaagacc ctcgtctatg tcgtcaatcg cgaacaggaa atcgaggagc
     2041 gaccggtgac actgggcttg gaaacaccag gcaaatatga ggtcgttgac ggcctgaagg
     2101 aaggcgattt ggtaatgatt ggcagccggt cgcaggtcaa gccgggccaa aaggtggaag
     2161 caaaactgat cgattcccag gcacaacaat gaacactcct cttactaaag agctattgga
     2221 atccctgcgg cggctggacg gctgcacgct agccaacgcc atcgaaacct tcaacgtgcg
     2281 cctgcgcaac gaaggattca ccgacggcgg actgcgctgc ttctttccgc tcctgccgcc
     2341 gatcgtcggc tacgccgcca cggtcaagat tcgcggctcg gcaccgccga cggcgggcgg
     2401 ttattacccc caacgcactg actggtggga gtatgtgcgg tccctgccgg cgccgcacat
     2461 cgttgtggcc caggacatcg cttcgcatcg cgggttgggc gcgatgttgg gccaggttca
     2521 tttcaacatc cagcgggcgc tgggcgccgt ggggatcgtc acgaacggcg ccgtgcgcag
     2581 cattcccacc gccgagacgc tggggttcca ggtttttgcc gccggcgtgt cggtgtctca
     2641 cgcttacacg cacctcctgg agttcggcac cccggtcgaa gtggacggcc tcaaggttca
     2701 ctccggcgaa ctgctgcatg gcgaccagca cg
//
LOCUS       DATCGS010000249         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_468494, whole genome shotgun sequence.
ACCESSION   DATCGS010000249 DATCGS010000000
VERSION     DATCGS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468494"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="VIL39_05055"
     CDS             <1..1688
                     /locus_tag="VIL39_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEY5480828.1"
                     /translation="VSLVWGASPGDRQTISIRTLTGQCVKVPLKFTANADTNDGRFEV
                     VGTGSGTFHIGAVSMMPADNVSGFKAASIRLLKEQGISIARWPGGNFVSAYDWRDGIG
                     DADQRPSRRELAWSGMESNDMGIDDFMTFCRLLSAEPYIAVNTGFGDAHSAAEEVEYV
                     NGPTTSRLGQLRAANGHPVPYGVRIWGIGNEMYGPWQWGHMAVTQYPEKHNLIVKAMR
                     KVDPAIKVIASSATPEELSWTYIENRQLGTFPGREQVNDKVPFGFGTKYDWTGALLER
                     SADYIDYLGEHFYGYPNLAINAEKQAFVEADDAIVDRVRRMSNKVQMKFEAWEEYLKR
                     MPSLKGKDIRFAFDEWAPRNRAVSSTSAAPAVSNPMLNPLTNALVYHEFFRHSDMVAL
                     GVATGGMGTLAMDAYGDAAGFRMEGLVIKVLHDHFAGRMPVAVTGNSPQHVVKGTLGV
                     DLPARASGSPTYPLDVFAALSADRKKLAISVVNPTETTQVCELNLTGVQPGGAARLWQ
                     LTAPAGAAPPPAGPGRGGFSGPPATMAEDSLAQAPRRITLPPSSISVYEFEVK"
     gene            complement(1881..2702)
                     /locus_tag="VIL39_05060"
     CDS             complement(1881..2702)
                     /locus_tag="VIL39_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5480829.1"
                     /translation="MGAVFEILHRMAALFRGVLPKLIEDYRQTPVRHADETGWRTDGR
                     SGYAWLFASATVSLFLFRATRLAQVPKEVLGEQALAGVLVVDRYAAYNRAPCALQYCY
                     AHLMRDVEDLAEEFPGEAEVTAFTSTLIPLLAEAMHLRGRPLADAEYYDQSRPLQQQI
                     VDLIEQPAQHLGVRQVQGIFRDNAHRLYHWVENRAVPADNNRAERELRPTVIARKVSF
                     GSQSDAGAKTRGVLMSAVHTLVKRVPDPEAHFKAVLDQLAADPTQDPVALLLNTS"
     gene            complement(2703..3257)
                     /locus_tag="VIL39_05065"
     CDS             complement(2703..3257)
                     /locus_tag="VIL39_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480830.1"
                     /translation="MSVNLNLCESCLEKQRTIDGLKQEVQSLREKVHRLERKAEAGPF
                     GSSTPSAKIPVKANTAEEHHSKPGGAKPGHTGHGRKAVEDSAAVRIETIAVGPVCPHC
                     TGALEHKEYRDRSVIESQPLRAEPILYQLERARCPRCGKTVQARAPEVLPKSLYGNQL
                     VTQVVFLHYRQGIPMGRLCDQLAI"
     gene            complement(3254..>3884)
                     /locus_tag="VIL39_05070"
     CDS             complement(3254..>3884)
                     /locus_tag="VIL39_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEY5480831.1"
                     /translation="EPLRLEVVRGGQADRGGDHALFKELLDRHHYLGYRVPFGASLRY
                     LVRSERGPQNVLACLLWSSPAWRMAARDGWIGWNDGERARNLQYIVNNSRFLILPWVR
                     VKGLASKILGRCARQLPRDWEKLYGYRPLLLETLVDAERFRGTCYRAANWIALGQTTG
                     RGRMDRDHEAHGAAVKDLYVYPLCRHVPQRLRSATAPVWQEPEQEASQP"
BASE COUNT          702 a         1260 c         1232 g          690 t
ORIGIN      
        1 acgtcagtct ggtctggggc gcgagtcctg gagatcgcca gacgatcagc atccgcacgc
       61 tgaccggcca gtgcgtcaag gtgccgctga agttcacggc aaacgcagac actaacgacg
      121 gccgcttcga ggttgtcggg acaggcagcg gcacgttcca catcggggcc gtttccatga
      181 tgcccgcgga caacgtctcg ggttttaagg ctgcgagcat ccgtctgctg aaggagcagg
      241 gaatctcgat cgcgcgctgg cctggcggga acttcgtgtc cgcctacgac tggcgtgacg
      301 gcataggcga cgccgatcag cggccatccc ggcgcgagct tgcatggagc gggatggagt
      361 ccaacgacat gggcatcgat gacttcatga ccttctgccg cctgctgagc gcagagccct
      421 acattgcagt gaacacgggg ttcggcgacg cccactcggc cgcggaggag gtcgagtatg
      481 tgaatggccc caccaccagc cggctgggac aattgcgcgc agctaacgga catcccgtgc
      541 cctacggcgt gaggatctgg ggaattggca atgagatgta cgggccgtgg cagtggggcc
      601 acatggccgt cacccaatac ccggaaaagc acaacctcat agtcaaggcc atgaggaagg
      661 tcgatccggc gatcaaggtg atcgcctcca gcgccactcc cgaggaacta tcttggacct
      721 acatcgaaaa ccggcagttg ggcaccttcc caggacggga gcaggtgaac gacaaggtgc
      781 cgttcggctt tgggacgaag tatgactgga cgggagcgct gttggagcgt tccgccgact
      841 atatcgacta tctcggtgag cacttctacg gctatcccaa tctcgcgatc aacgccgaga
      901 agcaggcgtt cgtggaggct gacgacgcga ttgtcgacag agtgcgccgc atgtcgaaca
      961 aagtgcaaat gaagttcgag gcatgggaag agtacttgaa gaggatgccc tcgctcaaag
     1021 gcaaagacat caggttcgcc ttcgacgaat gggcgccgag gaaccgggcg gtgagttcca
     1081 cgagcgccgc tcccgccgtc agcaatccga tgttgaatcc cctgaccaac gccttggtct
     1141 accacgagtt cttccggcac tcggacatgg tggcgttggg cgtggccacg ggaggcatgg
     1201 ggacgcttgc gatggacgcc tacggcgatg cggccgggtt ccgcatggaa gggctggtta
     1261 tcaaggtgct gcacgaccac ttcgccggca gaatgccggt tgccgtcacc ggcaactcgc
     1321 cccagcacgt cgtcaagggg actctcgggg tggatctccc ggcgcgtgcg tccggaagtc
     1381 caacctaccc gcttgacgtg ttcgcggcgc tgagcgccga tcgcaagaaa ctcgctattt
     1441 cggtggtgaa ccccaccgaa acgacgcagg tttgtgagct gaatctgacc ggcgttcagc
     1501 ccggcggggc tgcccggctt tggcaactga ccgctcccgc cggggctgcg ccgccgccgg
     1561 ccggccccgg acgcggcggc ttcagcggcc ctccggcgac gatggcggaa gactccttgg
     1621 cacaagcgcc acgcagaatc acgttaccgc ccagcagcat cagtgtttat gaattcgaag
     1681 tgaagtagtc cacgtgaggt cgcaactcgt ggtgcggatc aggccgaggc tgcgtgctac
     1741 ggtgatgatg gaactgcaat gaatcagggc gtcaatcggt aatccctcag tctcggggac
     1801 gggcttgagg ttgcgcgcac tggtgcggca gcggcggagg gtactgctcg atggcggtag
     1861 attggacggc tggattcggc tcaactggtg ttgagcagca aggcaacggg gtcttgcgtg
     1921 gggtcggccg cgagttggtc gagcaccgcc ttgaagtgtg cctccggatc ggggacccgt
     1981 ttgaccagcg tgtggactgc gctcatcagt accccgcgcg ttttcgcgcc ggcatcggac
     2041 tgcgagccga agctcacctt gcgggcgatc accgtcgggc gcagttcacg ctccgcccgg
     2101 ttgttgtcgg ccggcaccgc acggttttcc acccagtggt acagacgatg ggcgttgtcg
     2161 cggaagatgc cctgcacctg gcggacaccc aagtgttggg cgggttgctc aatcagatcc
     2221 acgatctgtt gctgaagcgg gcgggactga tcgtaatact cggcgtcggc aagcggccgc
     2281 ccgcgcaagt gcatggcctc ggccagcagc ggaatcaacg tggaagtgaa ggctgtcacc
     2341 tcggcctcgc cgggaaactc ctcggccaag tcttccacgt cccgcatcag atgcgcataa
     2401 cagtattgca gcgcgcaggg tgcccggttg taggcggcgt agcggtccac caccagcacg
     2461 ccagccaagg cttgctctcc cagcacctcc ttgggcacct gcgccaaacg cgtcgcgcgg
     2521 aacagaaaca ggctcaccgt cgcgctggcg aacagccagg cgtagcccga gcggccgtcc
     2581 gtacgccaac ccgtctcatc ggcgtgccgc accggggttt ggcggtaatc ctcgatcagc
     2641 ttcggcagca caccccggaa caatgccgcc atgcgatgca gaatctcaaa taccgcgccc
     2701 agtcagatgg ccagttgatc gcacaaccgc cccatcggaa tcccttgccg gtaatgcaga
     2761 aacaccacct gggtgaccag ttgattgccg tacagactct tgggcaacac ctccggcgcg
     2821 cgcgcctgaa ccgtcttgcc gcaacgcgga cagcgcgccc gctccaactg atagaggatc
     2881 ggttccgcgc gtaacggctg gctttcgatg accgagcggt cgcggtactc cttgtgctcc
     2941 aacgctccgg tgcagtgcgg acacaccggc cccacggcga tggtttcgat ccgcacggcg
     3001 gcgctatcct ccactgcctt gcgcccgtga ccggtgtgcc ccggcttggc cccgcccggc
     3061 ttgctgtggt gctcctcggc cgtgttggct ttcaccggaa tcttcgccga cggcgttgag
     3121 gaaccaaacg gtccggcttc ggccttgcgc tccagccggt ggaccttctc ccgcaggctt
     3181 tggacctcct gcttcagccc gtcgatggtg cgctgtttct ccagacagct ttcgcacagg
     3241 ttgaggttca cgctcacggc tggctcgcct cttgctccgg ctcttgccac acgggcgccg
     3301 tggcgctccg taagcgttgt ggaacgtgac ggcacagcgg atacacgtag agatctttga
     3361 cggctgcccc gtgcgcctca tgatcgcggt ccatgcggcc gcgcccggtg gtctgcccca
     3421 gcgcaatcca gttcgccgcc cggtaacagg taccgcggaa tcgttccgca tccaccagtg
     3481 tctccagcaa cagcggccgg tagccgtaca gcttctccca gtcccgcggc aactggcgcg
     3541 cgcaccgccc cagaatcttg ctggccagtc ccttcacccg cacccagggc aggatgagga
     3601 agcggctgtt gttgacgatg tattgcaggt tgcgcgcccg ctcgccatcg ttccagccga
     3661 tccatccatc gcgcgccgcc atcctccagg ccggcgagga ccacagcagg caggccagca
     3721 cgttctgtgg ccctcgctcg ctacgcacca gataccgcag gctggcgcca aacggcaccc
     3781 gatagcccag atagtgatgc cggtcgagca actctttgaa cagcgcgtga tccccgccgc
     3841 ggtccgcctg ccccccgcgc accacctcca ggcgcaacgg ttcc
//
LOCUS       DATCGS010000250         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_269547, whole genome shotgun sequence.
ACCESSION   DATCGS010000250 DATCGS010000000
VERSION     DATCGS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269547"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VIL39_05075"
     CDS             <1..292
                     /locus_tag="VIL39_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480832.1"
                     /translation="EEQPVPPSVRAGRPICPHLEGLILHCLERDPKDRPQSAHELIAL
                     LSASPRIADWNVEQRAAWWVAHREAINRARAAEAEPIETAKAVNIEIEDRAL"
     gene            complement(306..2909)
                     /locus_tag="VIL39_05080"
     CDS             complement(306..2909)
                     /locus_tag="VIL39_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEY5480833.1"
                     /translation="MANESHNESAMPRRKFLQLSAATMGAVGSLGAAPAAEFEHAHSQ
                     HSTGAKTARAPGRHKRRPYNSEYAGECLNQVAFPLGGIGAGMICLEGTGALSHVSLRN
                     HPDVFNEPCVFAAISVKGKRPAARVLEGPVPGRKLFGAHGTGNGAGGSTYGLPRFANA
                     AFKTRFPFGVVTLTDPQVPLGVEITGWSPFEPGDADNSSLPVAALEYRFTNRSKGPIE
                     AVFSFHAKNFMAAGGDPQAVRPVEGGFILWGGASKQKPVEEGAFSATVSEPGAKVNYG
                     WFRGGWWDALTMAWKDVTEGACYDRAPLTEGEPSPGASLFVPLQLAAGASKTVALRLA
                     WFSGQTNLHVGKDLAGFKLEPDQAGKYRPWYAGRFTDINGVTFYWRDHYEELRQKASR
                     FSDCFYDSTLPPEVIEAVAANLTILKSPTVMRQTDGRLWSWEGCSDNAGCCAGSCTHV
                     WNYAQAIPHLFPTLERTLRETEFGPSQNEAGHQQFRSGLPIRPVEHDFHAAADGQLGG
                     IMKVFREWRISGNTEWLRGLWPKVKRSLDYCIETWDPGHKGVVEEPHHNTYDIEFWGP
                     DGMCTSFYLGALQAAVIMGKALGDEVPLYAELLAGGSRRAESELFDGEYFIQRIEWKN
                     LRAKNPMENKSMVGSYSPEAVAILEKEGPKYQYGNGCLADGVLGSWLALVCGVGQVLA
                     APKVASHLQAVHKYNLKHDLSDFANPQRPSYACGAEGGLVLCTWPKGGELSLPFVYSN
                     EVWTGIEYQIASHLMRMGRVQEGLEVVRICRDRYDGRVRNPFNEYECGHWYARAMSSY
                     ALLFGLSGARYDAVEKVLYLEPGIRGDFHCFFSTATGYGTVGVRKGKPFYEAKAGKLE
                     IREVRYLMRDA"
BASE COUNT          601 a         1077 c          941 g          550 t
ORIGIN      
        1 ggaggaacag cccgtgccgc caagcgtcag agccggccgc ccgatttgcc cacatctcga
       61 aggcttgatc ttgcattgcc tggagcgaga cccgaaggac cgcccgcaat cggcccacga
      121 gctgatcgca ctgttgtctg ccagcccgcg catcgcggat tggaacgtcg agcaacgggc
      181 cgcctggtgg gtggcccatc gtgaagccat caaccgtgcc cgggccgccg aggcggagcc
      241 gatcgagacc gccaaggccg tgaatatcga aatcgaagac cgcgcgctgt gacccgtatc
      301 acgcgtcacg catcacgcat caagtaccta acctcccgga tttccaactt ccccgccttc
      361 gcctcataga acggtttgcc cttccgcaca cccaccgttc cgtagccggt cgcggtggag
      421 aagaagcagt ggaagtcgcc gcggatgccg ggttcgaggt aaagaacctt ttccacggcg
      481 tcgtagcgcg cgccgctcag cccgaataaa agcgcgtacg aggacatggc ccgcgcatac
      541 cagtggccgc attcgtattc gttgaacgga ttgcgcacgc ggccgtcgta ccgatcccgg
      601 cagatgcgca caacctcgag cccctcctgc acgcgcccca tccgcatcag gtgggaagcg
      661 atctggtatt cgatgccggt ccacacctcg ttcgagtaca caaaaggcag ggacagctcg
      721 ccgcccttgg gccaggtgca gaggaccagc ccgccttccg ccccgcaggc gtacgatggt
      781 cgctgcgggt tggcgaagtc ggagaggtcg tgcttcaggt tgtatttgtg caccgcctgc
      841 aagtggctgg cgaccttcgg ggccgcgagc acctggccga ccccgcacac cagcgccagc
      901 cacgaaccca gcacgccgtc cgccagacag ccgttgccat attggtactt cggcccttcc
      961 ttctccagga tcgccaccgc ttccggcgag tagctcccaa ccatgctctt gttctccatc
     1021 gggttcttcg cgcgcagatt cttccactcg atgcgctgga tgaaatactc cccgtcgaaa
     1081 agctcagact cggccctccg gctgccgccg gccagcagct cggcgtagag cgggacctcg
     1141 tcaccgagcg ccttgcccat gatcaccgct gcttgcaatg cgcccaggta aaagctcgtg
     1201 cacatgccgt ccggccccca gaactcgatg tcgtacgtat tgtgatgcgg ctcctccacc
     1261 acgcccttgt gccccgggtc ccacgtctcg atgcagtaat ccaggctgcg cttcaccttc
     1321 ggccacagcc cgcgcagcca ttcggtgttg ccgctgatgc gccactcgcg aaacaccttc
     1381 atgatccccc caagctgtcc atcggcggcg gcgtggaagt catgttccac cggccggatg
     1441 ggcagcccgc tgcggaactg ctggtggccg gcctcgttct gcgacggacc aaactcggtc
     1501 tcgcgcaagg tccgctccag ggtcggaaag agatgaggga tggcctgcgc gtagttccaa
     1561 acgtgagtgc aagagccggc gcagcagccc gcattgtcgc tgcaaccttc ccaggaccag
     1621 agcctgccgt cagtctgccg catgactgtg ggtgacttca ggatcgtgag gttcgcggcc
     1681 accgcttcaa tgacctcggg cggcagagtc gagtcataga agcagtcgct gaaccggctg
     1741 gccttctgcc gcaactcctc gtaatggtcg cgccaataga aggtaacccc gttgatgtca
     1801 gtaaaacgcc cggcatacca cgggcggtat ttgccggctt ggtccggctc aagcttaaag
     1861 cccgcgaggt ctttgccgac gtggaggttc gtctgcccgg agaaccacgc cagccgcagc
     1921 gcgacagtct tcgatgcgcc ggccgcaagc tggagcggaa cgaacaagct cgccccggga
     1981 ctgggttcgc cctcggtaag cggcgcgcga tcgtagcacg ccccctcagt cacatctttc
     2041 caagccatag tcaacgcgtc ccaccacccg ccgcggaacc agccatagtt caccttcgcg
     2101 cccggctcgg aaacggtcgc cgaaaacgct ccctcttcca ccggcttctg cttcgacgca
     2161 ccgccccaca agatgaaccc gccctcgacg ggccgcactg cctgcggatc accgcccgca
     2221 gccatgaagt tctttgcgtg gaaggaaaac accgcctcga tcgggccttt gcttcggttg
     2281 gtgaaccggt attccaatgc ggcgaccggg aggctggaat tgtccgcgtc gccgggctcg
     2341 aacgggctcc agccggtgat ctcgacaccc agcggaactt gcggatcagt gagcgtcacc
     2401 acgccaaacg ggaaccgggt cttgaacgca gcattggcaa agcggggcaa accgtaggtg
     2461 gagccgccgg ccccgttgcc ggtgccgtgc gctccaaaga gtttccggcc cggcaccggt
     2521 ccttccaata cgcgcgcggc agggcgtttg cctttgaccg agatggccgc gaagacacac
     2581 ggctcattga agacgtccgg gtggttgcgc agcgacacat gcgagagcgc cccagtgccc
     2641 tccaggcaga tcatgcccgc gccgatgccg cccagcggga aggctacctg gttcagacac
     2701 tcgccggcgt attcgctgtt atacggacgc cgcttgtgcc ggcccggcgc gcgcgcagtc
     2761 ttggctcccg tggaatgttg gctgtgggcg tgctcgaatt ccgccgccgg cgcggcgccc
     2821 agactgccaa ccgcccccat cgtcgcggcg gacaattgca gaaacttccg gcgcggcatg
     2881 gccgattcat tatgcgattc atttgccata atcattcctt tggttcttag cagtaacgga
     2941 cgccccgttt atgcgcgaaa gcgggtccga actcaagccg gctttgcggc agcatcgaga
     3001 agcgcccgcc agcgcggtgg acagccggag cgccttcctg gaaaaatgtg attgcgaccg
     3061 aagccctcac cggagcgcaa cgaaaacacc ggggaaacac tcccgaaccc caaagaatct
     3121 aaaagcgatg tacaagggat agataaggga tgtacaaggg ttgtacaag
//
LOCUS       DATCGS010000251         5615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_121910, whole genome shotgun sequence.
ACCESSION   DATCGS010000251 DATCGS010000000
VERSION     DATCGS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121910"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(582..992)
                     /locus_tag="VIL39_05085"
     CDS             complement(582..992)
                     /locus_tag="VIL39_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480834.1"
                     /translation="MCAESHQTEGMSRGGLRVEPPLRQGRSGGYSGEAKRAHGEAEAE
                     RLLARGLAALGLAEGQLAETRKGAWEKEALAWWLCQHITSRRRWVSERLGMGDESRVT
                     QAIGRLKRKGQPELERLKRRLEQVYENHNGEVEV"
     gene            991..1914
                     /gene="larE"
                     /locus_tag="VIL39_05090"
     CDS             991..1914
                     /gene="larE"
                     /locus_tag="VIL39_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY5480835.1"
                     /translation="MVSEKYERLRGLLRAYGSCLVAYSGGVDSAFLACVAHEVLGDKS
                     LAAIADSPSLPRREFEQAMSLGRQFGFPVRVLRTREFENASYLANPDNRCYFCKHELF
                     TELAPLAKAEGLAVIAYGENASDIGDFRPGAQAAAEFQVRAPLKEAGLTKADIRTLSA
                     QLGLPTADKPQMACLSSRVPYGERVTPHKLGMIEQAENVLRDLGFYDVRVRHHELRSA
                     EFLSTINSQLSTTRHLARIEVGPAEMQKLLENGLSLKVAEALKQIGYAHVTLDLQGYR
                     RGSTNEVLPLSGSQDAAAGQQPKFSPQDPKT"
     gene            2063..3340
                     /locus_tag="VIL39_05095"
     CDS             2063..3340
                     /locus_tag="VIL39_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5480836.1"
                     /translation="MPQFSYKARRRTGEVVQGVLEVADRSAALVQIERLGLFPVAVDA
                     AKASAIAAAAERQPGRKTDWRALLPPTLRAAMAQKRKPKLQELATFTQQLANLLSSGM
                     PLTVALNSMTHLESKGIPAEVSKELKQDVMEGRSLSDSMAKQPRIFSDLYVNMVRAGE
                     SSGALVEVLRRMADHFERFAQVQSKFTSALVYPAFVSVVGIAIMFFFMSYMLPKFMTI
                     FEGMNVPLPLMTQILVKISHVFAAYWWLMLATVVAAVILFKRFQATPQGRRKIDHWRM
                     NAPLFGKVVKLNIFGQFARTLSTLLENGVPVLTALKITEQIIPNSIVKEAIAKTREEV
                     TDGKTIAQPLARSKLFPQLMVDLVKIGEDTGDVPGALKNVADTYENELSIALRVMTNM
                     IEPTLIIVMAVGVGFLLLSVLSAMFAITSNIAR"
     gene            3347..4033
                     /locus_tag="VIL39_05100"
     CDS             3347..4033
                     /locus_tag="VIL39_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5480837.1"
                     /translation="MSPKSQVQSPKPAPRFAACGLQLEPPGHASRITHHVSRITHHAP
                     RITHPPRAFTLIEIMIVVAIMGIVLTMSVPIVYKTWRKVPMREAVKDIVEVCSHARAR
                     AIMNGVETEVVFHPRENRLGLAGGASPARSAGVAGGTPGVNTSTASGSGLSAQLSNAV
                     IIETLDINMSGIEYNDADEAKIHFHPNGTSDEMRMLLFDGRDRIGIELEITTGLANVV
                     NNPLREWTRR"
     gene            4030..4503
                     /locus_tag="VIL39_05105"
     CDS             4030..4503
                     /locus_tag="VIL39_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480838.1"
                     /translation="MNCRVKSGEWRVASGDTDHASRITHHASRITPSHAAFSLMEVMI
                     ASFIFFMAIFAILAMISNVLRNARSLRRVELDAGMVAAQVSNTNKLTEGTQSGDFGNM
                     YQDYSWEADQYEIGTNGLWQVDIVVHRRGLQKPADVMSIWLFRPESKSGFGPRSR"
     gene            4503..5444
                     /locus_tag="VIL39_05110"
     CDS             4503..5444
                     /locus_tag="VIL39_05110"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEY5480839.1"
                     /translation="MQTLPPSCRLLRPRPASAACSRAWVSGFGFRPADFLRPSDFGLR
                     TSRPRRAAFTLVEIMIAIGILSIILSAIYSSWTAILRSSKTGLEAAASAQRARMTIRE
                     LEESLGSAQSFAANLPYYAFVAENGNEASLSFVSRLSKSFLRSGKFGDLDMRRVTFSV
                     EPSPDGGRQLVLRQNPLVMELDTDEKEHPIVLAKNVKGFEMQFWATDKKPPDWVDEWR
                     EQKTNQLPSMVMITLKLADSPQSTRITEEITRIISIPSMTVQPVWQVTRGPGGPGGPG
                     GLVPPGGVVPPGGVVPPGGLGPPGGLGPPGGSIVRPR"
BASE COUNT         1097 a         1861 c         1578 g         1079 t
ORIGIN      
        1 aaggcttatg gaaaacactg aactttgaac ctcgaacctc gaaccttgaa cagccgggca
       61 aagccaccga aagccggagg tgattcgtga ttcgtgattc gtgactgggc gggcaagcca
      121 ccccaaagcc acaattgggc aaaagctgaa atgctgaaag ctgaaagctg aaatcagaaa
      181 gagagctgca cagagccacc caaagccacc ccaaggccac cccaaggcct gttgataggc
      241 accgcatagg caccccaaag ccaccccaag gctaccccaa ggctacccca aggctacccc
      301 aaggctacac caaagccagg ggagccgcat tctggaaccg tttccgcgca acccccgggc
      361 gcatcctgat gccaccacca agactggctg ccctggagtt tggacgtttt cagcaaaact
      421 cagaaaccga atgccgggtt gaatggcgtg ccgccatttg cttcaggcgg ccccgcgggc
      481 tctggccgcg gggtgttacc gaaaagaact tccccggtcc agcttacttg ggcgggagca
      541 ttgccacgat gcggcataaa agggtcccgg aattcgcata ttcacacctc cacctctcca
      601 ttatggtttt catagacctg ctccagtcgc cttttcagcc gctccaattc cggctgccct
      661 ttccgcttca gccggccaat ggcctgcgtc accctcgatt cgtctcccat ccccaaccgc
      721 tcgctcaccc atcgccgccg cgacgtgatg tgctggcaca accaccacgc caacgcctcc
      781 ttttcccaag ctcccttcct cgtctccgcc agttggcctt ctgccagtcc cagcgccgcc
      841 aacccgcgcg ccagcaaccg ctcggcttcc gcctcaccgt gcgcccgttt ggcttccccg
      901 ctatagccgc cgcttcgccc ctgacgcagc ggcggctcca cccggaggcc tcccctcgac
      961 atcccctccg tttggtgcga ttctgcacac atggtatcgg aaaagtacga acggctgcgc
     1021 gggctcctgc gcgcttacgg ctcctgcctc gttgcctact ccggcggcgt ggactcggcc
     1081 ttcctcgcgt gcgtagcgca cgaggtgttg ggcgacaagt ctctcgccgc gattgccgat
     1141 tcgcccagcc tgccccgtcg tgaatttgag caggccatga gcctgggccg gcagttcgga
     1201 ttcccggtcc gcgtccttcg cacccgggaa ttcgagaatg ccagctacct ggccaacccc
     1261 gacaaccgct gttacttctg caaacacgaa ctgttcaccg agctggcccc gctggccaaa
     1321 gcggaaggtt tggccgtcat cgcctatggc gagaacgcca gcgatatcgg ggacttccgt
     1381 cccggtgcgc aggcggcggc ggaattccag gttcgcgccc ccctcaaaga ggccggcctg
     1441 accaaagccg atatccgcac gctctccgcc cagctcggtc tgcccacagc cgacaaaccc
     1501 cagatggcct gcctcagttc gcgggtgccc tacggcgaac gcgtcacccc gcataagctt
     1561 ggcatgatcg agcaggctga gaatgtcctg cgcgacctcg gcttttacga cgttcgcgtc
     1621 cgccaccatg aattgcggag tgcggaattc ctctcaacca tcaactctca actttcaacc
     1681 acccgccact tggcccgtat cgaagtcggc cccgctgaaa tgcagaagct cctggagaac
     1741 ggcctgtcac ttaaggtggc cgaggcgctc aaacagatcg gttacgccca cgtcaccctc
     1801 gacctccagg gctatcgccg cggcagcacc aacgaggttc tgccactgtc tggttcgcaa
     1861 gacgccgcgg ccggtcagca gcccaaattc agccctcaag acccgaagac ctgacggcct
     1921 gataaccgaa taagccggat cttccaacgc cgctcccggt ctccgtccct gccaagagaa
     1981 tggctgggcg ctccaaaaag ccgcttggtt taccgcaact tttggctaaa cttcttgttg
     2041 aagaaagaag cagttccgct ttatgccgca attctcatac aaagcgcgcc gccggacggg
     2101 ggaagtcgtc cagggcgtgt tggaagtcgc cgaccgctcg gcggcattgg tgcagattga
     2161 gcgcctgggg cttttcccgg tcgcggtaga tgcggccaaa gccagcgcga tcgcggccgc
     2221 cgctgagcgg caacccggcc gcaagacaga ctggcgcgcc ctgctgccgc ccaccttgcg
     2281 ggcggccatg gcccagaagc gcaaaccgaa actccaggag ctggccacct tcacgcagca
     2341 gttggccaac ctgctcagct ccggcatgcc gctgaccgtc gcgctcaaca gcatgactca
     2401 cctggagtcc aaaggtattc cggccgaggt cagcaaggag ctaaagcagg acgtgatgga
     2461 gggcaggagc ctgtccgact cgatggccaa gcagccgcgc atcttttccg atttgtatgt
     2521 caacatggtc cgggcgggcg agtccagcgg cgcgctggtc gaagtcttgc ggcgcatggc
     2581 cgaccatttc gagcggtttg cgcaggtgca gtcgaagttt acttccgcgc tcgtttatcc
     2641 ggcctttgtg tcggtggtgg ggattgccat catgttcttc ttcatgagct atatgctgcc
     2701 gaagttcatg accatcttcg aaggcatgaa cgtccccctg ccgttgatga cccagatcct
     2761 cgtcaaaatc agtcatgtgt tcgcggccta ttggtggctg atgctcgcga cggtggtcgc
     2821 cgccgtcatc ttgttcaaac ggttccaggc gaccccgcag ggccgccgca agattgatca
     2881 ttggcggatg aacgcgccgc tctttggcaa ggtggtcaaa ctgaacatct tcggccagtt
     2941 cgcgcgcacc ctctcgactc tgctcgaaaa cggcgtgccg gtgctcaccg ccctcaagat
     3001 caccgagcag atcattccca attccatcgt caaggaggcc atcgccaaga cccgcgagga
     3061 ggtcaccgat ggcaagacca ttgcccagcc gctcgcgcgc agcaagctct tcccccagct
     3121 catggtggac ctggtgaaga tcggcgagga caccggcgac gtgccgggcg cactgaagaa
     3181 tgtcgccgac acctacgaaa acgagctgag catcgccttg cgcgtaatga ccaacatgat
     3241 cgagccgacg ctcatcatcg tcatggccgt tggcgtcggg ttcctcctgc tgagcgtgct
     3301 ctccgccatg ttcgcgatta cgtctaatat cgcgcggtga ggtggcatga gtccaaagtc
     3361 ccaagtccaa agtccaaagc cggcgccacg gtttgcggct tgcggtttgc agcttgagcc
     3421 accgggccac gcatcacgca tcacgcatca cgtttcacgc atcacgcatc acgccccacg
     3481 catcacgcat cctccccgcg ccttcactct catcgagatc atgatcgtcg tggccatcat
     3541 gggcattgtc ttgaccatga gcgtgccgat cgtctataaa acctggcgca aggtcccgat
     3601 gcgcgaggcg gtgaaggaca tcgtggaagt gtgcagccat gcccgggccc gggcgattat
     3661 gaatggggtg gaaaccgagg tggtttttca tcccagggaa aaccgtctgg ggttggctgg
     3721 tggcgccagc ccggcacgct ctgcgggtgt ggcgggcgga acacccggcg tcaacacctc
     3781 gacggcctcg ggttccggcc tgtcggcgca gctatccaat gccgtcatca ttgagacgct
     3841 ggatatcaac atgtcgggta tagagtataa cgacgcggac gaggcgaaga ttcactttca
     3901 ccccaatggc acctccgacg aaatgaggat gcttctgttc gacggacgag accgcattgg
     3961 gattgagttg gagataacga ccggtctggc gaatgtcgtg aataatcccc tccgggagtg
     4021 gaccaggcga tgaactgccg cgtcaagagt ggcgagtggc gagtggcgag tggcgacacg
     4081 gatcacgcct cacgcatcac gcatcacgca tcacgcatca cgccatccca cgccgccttc
     4141 tcgctcatgg aggtcatgat cgccagcttc atattcttca tggccatctt cgcgattctg
     4201 gcaatgatat ccaacgtgct gcgcaacgca cgcagcttgc gtcgagtcga gctggatgcc
     4261 ggcatggtgg ccgcgcaagt ttcgaatacc aacaaactca ccgagggcac tcagtccggt
     4321 gacttcggga atatgtacca ggattactcc tgggaggcag atcaatacga gatcggcacc
     4381 aacggcctct ggcaggtgga cattgtggta caccgccgtg gactgcaaaa gccggcggat
     4441 gtgatgagca tctggctgtt tcggccggag tcgaagtccg gtttcggccc cagatccagg
     4501 tagtgcaaac cctccctcca tcctgtcgcc tgcttcgccc tcggcccgcc tcagccgcct
     4561 gctcccgggc ttgggtttcg ggcttcggat ttcgacccgc ggatttcctt cgcccttcgg
     4621 acttcggcct tcggacttcc cgcccccggc gggccgcctt cacgctggtc gaaatcatga
     4681 tcgccatcgg gatactctcc ataatcctgt cggcgatcta ttcgagctgg acggcgattt
     4741 tgcgctcctc caagacgggc ctcgaggcgg cggcctcggc gcagcgggcg cggatgacca
     4801 ttcgcgagtt ggaagaatca ctgggctccg cgcaatcctt tgcggcgaac ctgccgtatt
     4861 atgcctttgt tgccgaaaac ggcaatgaag cctccttaag ctttgtctcg cgactgtcca
     4921 agtcatttct ccgcagcggc aagtttggcg acttggacat gcggcgcgtc accttctctg
     4981 tcgagccgtc accggatggc gggcgccaac tggtcttgcg gcagaatcct ttggtcatgg
     5041 agctggatac cgacgagaag gaacatccga tcgtgctcgc caagaacgtc aagggcttcg
     5101 agatgcaatt ctgggccacg gacaaaaaac cgcccgactg ggtggacgag tggagggagc
     5161 agaaaaccaa ccaactaccc tcgatggtga tgatcactct gaagctggcg gacagtcccc
     5221 agtccacgcg gatcacagaa gaaatcaccc gcattatcag cataccctcc atgaccgtgc
     5281 aacccgtctg gcaggtgacg cggggcccgg gtggacccgg aggtcccggt ggcctcgtcc
     5341 ccccaggcgg cgtcgtccct ccaggcggcg tcgtccctcc aggtggcctc ggccctccag
     5401 gtggcctcgg ccctccgggc ggatcgatag ttagaccgcg atgaaactcc cccccacctc
     5461 atcccatcgc ccgaccgccg tgatcacgca tcacgcatca cgcatcccgc atcccgcatc
     5521 acgccgctct cagcaaggca tcgccctggt catcgtcatg atttcgatca tggtcctgac
     5581 gattctggcc gcggggtttg cctattccat gaagg
//
LOCUS       DATCGS010000252         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_666650, whole genome shotgun sequence.
ACCESSION   DATCGS010000252 DATCGS010000000
VERSION     DATCGS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666650"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1319)
                     /locus_tag="VIL39_05115"
     CDS             complement(33..1319)
                     /locus_tag="VIL39_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEY5480840.1"
                     /translation="MRYQPLNSRMFTSHRANLAKLLLPNSLAVVNANDILPTNADGTL
                     VLRPNSDLFYLAGVEQEESILLLYPDAHEENMREILFLRETNDLIATWEGHKLTKEEA
                     RKVSGIKRVEWLSQFQPLFRRLMCECEHAYLDANEHSRASIVVPTREARFVADTQARY
                     PLHDYQRLARLLHRLRAVKSEGEVALLKQACAITRAGFLRVCRFVKPGVTETEVEAEF
                     AHEFIRRGGNFAYSPIIACGKNACALHYLANDQRCRKGELLLLDVAASYANYNSDLTR
                     TIPVSGRFTRRQRQVYNSVLRVLRAASTAATPGKLPRQWQKEAEAMIEKELVDLGLLK
                     VAEIRKQDPDKPALKKYFMHGVGHPLGLDVHDVADTTQPIQAGWVLTVEPAIYIPAEG
                     FAVRLENDILVQANGNVDLMADIPIEAGEVERLMRR"
     gene            complement(1334..3706)
                     /gene="hypD"
                     /locus_tag="VIL39_05120"
     CDS             complement(1334..3706)
                     /gene="hypD"
                     /locus_tag="VIL39_05120"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HEY5480841.1"
                     /translation="MNDRTSQLRRQSLDAPPSISGERARLITEFYQANNGRYSVPVMR
                     ARAFHHLCREKTIHLGADELIVGERGPRPKAVPTFPELTCHSVEDLRILNSRPKTWYR
                     VDEETVRLYEEKVIPYWRGRSMRDRMFEELAPEWKEAYHAGIFTEFMEQRAPGHTVLD
                     GKIYSKGMEDFKRDIAQGVTNLDFLNDPEAYEKREALKSFEISCDAVILFAERHAALA
                     RELATKEGRPQRRAELLRIAEVCSRVPAHAPQDFHEALQYYWFCHLAVITELNGWDSF
                     NPGHLDQHLLPFYKRGLAEGTLTPESARELLECFFIKFNNHPAPPKVGVTAAESGTYT
                     DFANINIGGLLADGTNGANEVSDMLLDIVDEMHLLQPSTNIQLSRKSPDAFFKHALRV
                     VSRGYGFPSIFNADTVVQEQLRQGKTLEDARAGGCSGCVEVGAFGKEAYILTGYFNLV
                     KVLELVLHDGLDPRTGKQLGLQTGRPDSFQNFEQLFAAFRKQLHHFIEIKVRGSRLIE
                     RMYADDMPAPFLSVLIDDCILKGRDYNAGGARYNNTFIQMVGLGSITDCFSALRQFCF
                     DGEQPAPSMPLSQLVERLDRNLAGDEVLRQRLVNRTHKYGNDDNYADDLMLEVFNACF
                     EEVDGRPDARGGRYRVEMLPTTCHVYFGSVTGAMPDGRLAGLPLSEGISPVQGADRHG
                     PTAVFKSAAKMDHVKTGGTLLNLKFTPGLVATEEGLDKWAHLVRSYFKMDGHHVQFNV
                     VTAATLRKAQAQPEQHRDLIVRVAGYSDYFCDLSTELQEEIIARTEHPEA"
     gene            complement(3805..4710)
                     /locus_tag="VIL39_05125"
     CDS             complement(3805..4710)
                     /locus_tag="VIL39_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEY5480842.1"
                     /translation="MQNGLLFNIQKYSVHDGPGIRTTVFLKGCPLHCAWCHNPEGISR
                     QREIVVLESRCIGCGQCRLACRHGAVIPGDGALPARSEVCELCGACVEACPTQGRQII
                     GQEMSVSQVLETVLQDQVFYEDSGGGVTFSGGEPLSQPDFLRGLLGACRGRGLHAAVD
                     TCGLARQEDLLAVAALTDLFLYDLKFMDDAKHRRYTGVSNALILDNLQALGRVHGRIW
                     VRVPIIPGINDDPADLEAAARFAAAVSGVQQVNLLPFHRTGLPKLRRLGQDSGLAEVQ
                     PPSAAAMNRAADIFTRAGLTVKQGG"
     gene            complement(4632..5054)
                     /locus_tag="VIL39_05130"
     CDS             complement(4632..5054)
                     /locus_tag="VIL39_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480843.1"
                     /translation="MFMPIMTLKSRLTGLIAIATCCLARLSAQAEYHGFNTASGADGM
                     VQEVRWPVPFPVIASFTLLANGSFNLGGTGAVNQAYVLLASSNLVPPVSCCPKGWPTS
                     AGEAKLPLALIASCKTACFSTFRNTQCTTGRVSGRLSS"
     gene            complement(5393..>5606)
                     /locus_tag="VIL39_05135"
     CDS             complement(5393..>5606)
                     /locus_tag="VIL39_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480844.1"
                     /translation="EESEAIQDSMSTKESWEWELWRHAPDTRRTLPVDNFETPSESRK
                     QKIESRDKGGRPGKPPKATFRQKLKS"
BASE COUNT         1032 a         1681 c         1742 g         1151 t
ORIGIN      
        1 ttcggacttc ggacttcagc cccgcccgac tctcaacgcc gcatcagcct ctccacctcc
       61 cccgcctcga tagggatatc cgccatgaga tctacattgc catttgcctg caccaggatg
      121 tcgttttcga ggcggacagc gaagccctcg gctgggatgt agatggccgg ctcgacggtg
      181 agcacccagc ccgcctggat gggttgcgtc gtgtcggcga catcgtggac gtccaacccg
      241 agcggatggc caacaccgtg catgaaatac ttcttgagcg ctggcttgtc cgggtcctgc
      301 ttcctgattt cggccacttt cagcagcccg aggtccacca gctccttctc gatcatagcc
      361 tcagcttcct tttgccattg cctgggcagc ttgccgggcg tggccgccgt gctggctgcg
      421 cgcaaaacgc gcaggacgga gttatagacc tggcgttgcc ggcgggtgaa acggccgctg
      481 accgggatgg tgcgagtcag gtcggaattg tagttggcgt aagaggccgc cacgtccagc
      541 aggagcaact ctcctttccg gcaacgctgg tcgttggcca gataatgcaa ggcacaggcg
      601 ttcttgccgc aggcgatgat cggcgaatag gcgaaattac cgccgcgccg aatgaattcg
      661 tgggcgaact ccgcctcgac ctccgtctcg gtcacccccg gcttcacgaa cctgcaaacc
      721 cgcagaaagc cggccctggt gatcgcgcag gcctgcttga ggagcgcgac ttcgccctcg
      781 gacttgaccg cacgcaaacg gtgcaacaga cgcgccaggc gctgataatc gtgcaacgga
      841 tagcgggcct gggtatccgc aacgaaccgc gcctcgcgcg tggggaccac aatgctggcc
      901 cggctgtgct cgttggcatc cagataggcg tgctcgcact cgcacatcag gcggcggaac
      961 aagggttgaa actgggagag ccactcgacg cgcttgattc ccgatacttt acgcgcctct
     1021 tctttggtca gcttatgccc ttcccaggtg gcgatcaggt cgttggtttc gcgcaggaag
     1081 aggatttccc gcatgttctc ctcatgagca tctggataca ggagcaggat gctttcttcc
     1141 tgctcgacgc ccgcaagata aaacaggtcc gaattcgggc gcaggaccag ggtgccgtcc
     1201 gcattcgtgg gcagaatgtc gttggcgttt accaccgcga gcgagttagg gagcagcagt
     1261 ttggcgaggt tcgcccggtg gctggtgaac atccgtgagt tgagaggttg gtagcgcatg
     1321 cgtaataaag tggctaagct tccgggtgct cggttcgggc gatgatctcc tcctgtaact
     1381 cggtcgaaag gtcgcagaaa tagtcgctgt agccggcgac gcgcacgatc aggtcgcggt
     1441 gctgctccgg ctgggcctga gctttgcgca gcgtcgcggc ggtgacgacg ttgaactgga
     1501 cgtgatggcc gtccattttg aagtagctgc gaacgaggtg cgcccactta tccaggccct
     1561 cctccgtcgc cacgaggccg ggggtgaact taaggttgag cagcgtgccg ccggtcttca
     1621 cgtggtccat cttggctgcg ctcttgaaga ccgcggtcgg cccgtgacgg tcagcgccct
     1681 gcacgggact gatgccttcg gacaacggca ggccggcaag gcggccgtca ggcatcgcgc
     1741 cggtcacgct cccgaagtac acatgacagg tggtgggaag catttcgacc cgatagcggc
     1801 cgccgcgggc gtcgggcctg ccatccacct cttcgaagca ggcattgaac acctccagca
     1861 tcaggtcatc ggcgtaatta tcgtcgttgc cgtatttatg ggtgcggttg actagccgct
     1921 gacgaaggac ttcatcgcca gcaagattgc ggtccaggcg ttccaccagt tggctgaggg
     1981 gcatggaagg ggctggttgc tctccgtcaa agcagaactg ccttagcgcg ctaaagcagt
     2041 cggtaatgct ccccagcccg accatctgga tgaacgtgtt gttatagcgc gcgcccccgg
     2101 cgttgtaatc ccgcccctta aggatgcaat cgtcaatcag cacgctgaga aacggcgccg
     2161 gcatgtcatc ggcgtacatc cgctcgatga gccggctgcc ccgaacctta atctcgatga
     2221 aatggtgcag ttgctttcgg aaggcggcga acagttgctc gaagttctgg aacgagtcgg
     2281 gccggccagt ctgcaacccc aactgcttgc ccgtcctcgg gtccagccca tcgtgcagaa
     2341 cgagttccag gaccttgacc aggttgaagt agccggtgag aatgtaagcc tctttaccga
     2401 atgcccccac ttccacgcag ccgctgcacc cgccggcgcg cgcgtcttcg agcgtcttac
     2461 cctgtcggag ttgttcctgg accaccgtgt cggcgttgaa gatggagggg aagccatagc
     2521 cccggctgac gacgcgcaac gcgtgtttga aaaaggcgtc agggctcttg cgcgagagct
     2581 ggatattcgt gctgggctga agcagatgca tctcgtccac gatgtccagc aacatgtcgg
     2641 aaacctcatt ggcgccgttg gtcccgtccg ccaggaggcc gcctatgttg atgttggcga
     2701 agtcggtata agtgccgctc tcggcggcgg tcacgcccac cttgggcggc gccggatgat
     2761 tgttgaactt gatgaagaag cattcgagca attcgcgagc ggattcgggt gtcaacgtcc
     2821 cctccgccag gccgcgctta tagaaaggaa gcaggtgctg atccagatgg ccgggattga
     2881 aggaatccca gccgtttagc tcggtaatca cggccaggtg gcagaaccag taatactgga
     2941 gggcctcatg gaaatcctgc ggcgcgtgcg ctgggacacg gctgcaaacc tccgcaattc
     3001 tcaacaactc cgcgcgccgt tgcggacggc cttccttcgt agccagctct cgtgccaggg
     3061 ccgcatggcg ttcggcgaag aggatgaccg cgtcgcagct aatctcgaac gacttgagcg
     3121 cctcgcgctt ctcataggct tccggatcgt tgaggaaatc aaggttcgtt acgccttgcg
     3181 caatgtcgcg cttgaaatcc tccatgccct tggaataaat cttgccgtcc agaacggtat
     3241 gcccgggagc gcgttgctcc atgaactcgg tgaagatgcc ggcgtgataa gcttccttcc
     3301 attccggggc cagttcctcg aacatgcggt cgcgcatcga gcggccccgc caatagggaa
     3361 tgaccttctc ctcgtaaagc ctcaccgtct cctcatccac ccggtaccaa gtcttgggcc
     3421 gcgaattcag gattctgagg tcctcaacgc tatggcaggt cagctcgggg aacgtcggca
     3481 ccgccttcgg acgcggccct cgctcgccga caatcagttc atctgcgccg aggtggatgg
     3541 tcttttcccg gcaaaggtga tgaaacgccc gggcgcgcat caccgggacc gagtagcggc
     3601 cattgttggc ctgatagaac tcggtgataa gccgcgcgcg ctcgccggaa atggagggag
     3661 gagcatcgag gctctgacgg cggagttggc tggtgcggtc gttcatatat agagcgtcat
     3721 cattgcttgc gccgggcgcc ctcacacggc gcggcttatg tcgctacgga cgtattgtcc
     3781 tcatttgaag actgatttca aggctcatcc tccctgctta acggtcaaac cggcccgggt
     3841 aaagatgtcc gccgcacgat tcatcgccgc ggcagaaggc ggttgaactt ccgccaagcc
     3901 gctgtcctgg ccgaggcggc gcagtttggg cagtcccgtt cgatgaaacg gaagcagatt
     3961 cacttgctgc acaccggaaa ccgccgccgc gaagcgggcg gcggcttcga ggtcggctgg
     4021 atcgtcgtta ataccgggga tgatcggcac gcggacccaa atccgcccgt gcacccgccc
     4081 cagcgcttga aggttatcca ggataagggc attggaaacg ccggtgtagc ggcggtgttt
     4141 ggcgtcgtcc atgaacttga ggtcatagag gaaaaggtcg gtcaatgctg cgacagccag
     4201 caagtcctcc tgccgggcca gaccacaggt gtccaccgcg gcgtgcaagc cgcggccgcg
     4261 acaggccccc agcaatcccc gcaaaaagtc aggctgagac aaaggctccc cacctgagaa
     4321 agtcacgcct ccgcccgaat cttcataaaa tacctggtct tgcagcacgg tttccaagac
     4381 ctggctgacg ctcatctctt gtccgatgat ctgccgcccc tgggtcgggc aggcttcgac
     4441 gcaggcgccg cacagctcgc acacttcact gcgcgccggc agcgcgccgt ctccgggaat
     4501 gaccgccccg tgccggcagg ccagtcggca ttgaccacag ccgatgcagc ggctttccag
     4561 caccacgatc tcgcgctggc gcgaaatgcc ctcggggttg tgacaccagg cgcaatgcag
     4621 cgggcagcct ttcaggaaga cagtcgtccg gatacccggc ccgtcgtgca ctgagtattt
     4681 ctgaatgttg aaaagcaggc cgttttgcat gaggcgatca gagccaacgg cagcttagcc
     4741 tcccccgcgc tagtcggcca tccttttggg cagcaagaca ccggcggaac caaattcgag
     4801 gacgccagca ggacataagc ctggttaacg gccccggtgc cgccgaggtt gaagctgcca
     4861 ttggcgagca gggtgaagct ggcgatcacg gggaacggaa ccggccagcg cacctcctgg
     4921 accatgccat cggccccgct ggcggtattg aaaccgtggt attccgcctg ggcggacagg
     4981 cgcgcgagac aacacgtggc gatggcgatc aaaccggtga ggcggctctt caatgtcata
     5041 attggcataa acacaggcgg gctatcaggc caagccacta accttaaccg ataccccggt
     5101 caggcagtat cgcacatttt aggtaggcgg cctcggggcc ttggtgtggc tttggtgaag
     5161 ccttggtgta gccttggtgt agccttgggg tggccttggg gtggccttgg ggtggccttg
     5221 gggtggcctt ggggtagcct tggggtagcc ttggggtagc cttggggtag ccttggggta
     5281 gccttggggt ggctttgggg tgcctatgcg gtgcctatca acaggccttg gggtggcctt
     5341 gggtggcttc gggcagccct ctttctgatt tcagctttca gctttcagca tttcagcttt
     5401 tcagcttttg ccgaaaggtg gctttgggtg gcttgcccgg tctcccgcct ttatctctgc
     5461 tttctatttt ctgctttctg ctttcactgg gagtctcaaa gttatctact gggagggtcc
     5521 ggcgtgtgtc cggcgcgtgg cgccagagtt cccattccca actttctttc gtggacattg
     5581 agtcctggat ggcctcgctt tcctcg
//
LOCUS       DATCGS010000253         3331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_269612, whole genome shotgun sequence.
ACCESSION   DATCGS010000253 DATCGS010000000
VERSION     DATCGS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269612"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VIL39_05140"
     CDS             <1..115
                     /locus_tag="VIL39_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY5480845.1"
                     /translation="RGKEIDEATFEANAQTIFDEAENRLHVQKAILSWLVS"
     gene            complement(382..1392)
                     /locus_tag="VIL39_05145"
     CDS             complement(382..1392)
                     /locus_tag="VIL39_05145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5480846.1"
                     /translation="MSNQSNPQPRRAFLKTLGAASAGAVFVGHTAPTAAADTPAIGPV
                     PKRKFGRHSEMISSLALGGATLAQAASIEEATRIVAAAIDCGVTFFDNAWEYSNGRAE
                     EWMGIAMQGKRDKVFLMTKVCTHNKGQETKEKALAMLEDSLRRLKTDHLDLWQVHQIL
                     TDAEADSIFIPGGVLEAIEQARKQGKIRYCGFTGHADPKAHLRVLAHHYPFDSVQMPL
                     SVFDGHDNGFQKLVLPELLKQGIAPLAMKTLAGNAKAITDGLVTSEECLRYALSLPIA
                     AVISGIDTLPWLEQNARVAANFKPLSRKEMAALEERCSSKKQYEYYRRWAYLDGHSPQ
                     LA"
     gene            complement(1503..2135)
                     /locus_tag="VIL39_05150"
     CDS             complement(1503..2135)
                     /locus_tag="VIL39_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5480847.1"
                     /translation="MKLNKFVNLLVIGLVLTVAASGCRKKPVNTTLLPGSRTPAPVTD
                     TGMGGAIKPAQPMSTDTGVPGGTSVLGGPGSLGDAHASNPAKLHDGWIPDAEALKAET
                     VFFDFDSSVVKASERPKVAAVADYLKANSPKAVKIEGNCDERGTEEYNRSLGERRALA
                     VREELIRLGIDLTRVDTISYGKDKPVAQGHDESAWRQNRRDDFIVLSPLK"
     gene            complement(2159..>3331)
                     /locus_tag="VIL39_05155"
     CDS             complement(2159..>3331)
                     /locus_tag="VIL39_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480848.1"
                     /translation="MKRTLSICNLFTLLATTMALAQEVLPTITGNFAPGNTPPILVSM
                     SGISGDAAAALHFDLYVQGFAFTNAEAAQYLISGSNNGNLQAKATDPHNKSTLVSKAY
                     SGASLRHQAHAFADDFVLALGRKGIAQTKIAFKGENSPTSEIYIADFDGQNAQAVTRD
                     NNLVAAPSWVPGYLALYYVSYKLNHADIFYHDLSTGARRAFARFGGSNMSPAASPRGS
                     KVAMILSKDGWTDLYVCDADGSNLKRLTKSPQDESSPCWSPDGKWICFAAKEKERRTL
                     CKVSPSGGPMQTIGTSGMPSPSEPDWSPDGKWIAFTAQYRSGFQICVVPATGGTATSL
                     VEGEDPSWAPNSRTLAFGRRRGGRYVLSLLDVPTKQYKDVSRISGISSQSQPSWAK"
BASE COUNT          620 a         1002 c         1024 g          685 t
ORIGIN      
        1 tcgcggcaag gagattgatg aagccacctt cgaggccaac gcgcagacga tcttcgatga
       61 ggccgagaac cgccttcacg tgcagaaagc gatcttaagc tggctcgtca gttagcaggc
      121 accgcgctcg tcaaactgac ggagggccag caggactggc attggggctt ctgccccctg
      181 acgccgcgca cgctcgacct cggccggcgg atcacctcgc agttccaccg tcgcgttccc
      241 tggctggggt agccttgggg tagcctcagg gtagcctcag ggtggccgca aacctcttac
      301 caaaagccga aaccgcgcgt aaaatcgcac gctataagcg ggctggctca tgatacgtca
      361 ccggatttgt gaccgggacc actaagccaa ttggggtgag tgcccgtcca gataggccca
      421 gcggcggtaa tactcatact gtttcttcga cgagcatcgc tcctccaggg cggccatttc
      481 cttgcgggag aggggcttga aattggcagc cacccgggcg ttctgctcca gccacggcag
      541 ggtgtcgatc ccggagatca cggcggcaat gggcaggctg agcgcatagc ggagacattc
      601 ctcggacgtc accagcccat ccgtgatggc tttggcgttg ccggccagcg tcttcatcgc
      661 tagcggcgcg atgccctgct tgagcaattc cggcagcacg agcttctgga acccgttgtc
      721 gtgcccatca aataccgaca agggcatttg caccgagtca aatgggtagt ggtgcgcgag
      781 gacgcggaga tgcgctttgg ggtcggcgtg cccggtgaag ccgcagtagc gaatcttgcc
      841 ctgtttcctg gcttgttcaa tcgcctcgag cacgccgccg ggtatgaaga tcgagtcggc
      901 ctcagcgtcc gtgagaatct ggtgcacctg ccaaaggtcc aggtggtcgg tcttgagccg
      961 ccggagggag tcttccagca tggccaacgc cttttccttg gtctcctggc ccttgttatg
     1021 ggtgcagacc ttggtcatca ggaacacctt gtcgcgcttg ccctgcatcg cgattcccat
     1081 ccattcctcg gcgcgtccgt tggaatattc ccaggcgttg tcgaagaagg taacgccaca
     1141 atcaatggcg gcagcgacga tgcgggtggc ttcctcgatg gatgcggcct gggccagggt
     1201 ggcgccgccc agcgcgagcg aggagatcat ctcggagtgg cggccaaatt tacgtttcgg
     1261 caccgggccg atggcgggag tatcggcggc ggcggtcggg gcagtatggc ccacgaacac
     1321 cgcaccggcg ctggctgcgc ccagcgtttt caagaaggcc cgacgaggct gtggattgga
     1381 ttggttggac ataagttgag taatataaaa ggggggcaaa agcccccctt gaaagttcgt
     1441 tatgccttgc ggaactgctc cgcaatagcc ttgccattca gggctttggc cccgccgccg
     1501 gattacttga gcgggctcag aacaatgaag tcgtcccggc gattctgtct ccaagcggat
     1561 tcatcgtggc cctgggctac gggtttgtct ttgccgtagc taatcgtatc aacccgggtc
     1621 aagtcaatgc ccaggcggat tagctcctcc cgcaccgcca gcgcccgccg ctcgccgaga
     1681 gaacggttgt attcctcagt gccacgctcg tcacaattgc cttcgatctt caccgccttg
     1741 ggcgagttgg ccttcagata atcggcgacc gccgccacct tgggtctctc ggaggctttg
     1801 acgaccgaac tgtcgaagtc gaaaaagacc gtctcggcct tgagtgcttc cgcgtccggg
     1861 atccagccgt cgtgcagttt ggccgggttg gaggcatgag catcccctag actgcctgga
     1921 ccgcctagaa cgcttgtgcc accgggcaca ccggtgtccg ttgacattgg ttgagcgggt
     1981 ttgatggcac ctcccatgcc cgtatccgtt acgggcgccg gcgtccgcga ccctgggaga
     2041 agcgttgtat tgacaggttt cttgcggcag ccggaggcgg cgacggtgag gactaatccg
     2101 atcaccaaca ggtttacaaa cttgttcagt ttcatatatg catctttcat ggacgggctt
     2161 atttcgccca actaggttgt gattgtgagc taattcccga aattcgggaa acatctttgt
     2221 attgttttgt cggcacgtca agcagagaga gcacataacg cccgccccgc cgccgcccga
     2281 aagccagcgt ccgggaattc ggcgcccagg acgggtcctc gccttcgacc agcgaggtgg
     2341 cagtgccacc cgtggccggc accacgcaga tctggaaccc ggaccggtat tgcgccgtga
     2401 acgcaatcca cttgccgtcg ggcgaccaat ccggctccga gggactgggc atgccgctgg
     2461 tgccgatggt ctgcatcggt ccgcccgaag gggacacttt gcaaagcgtc cggcgctctt
     2521 tctccttggc cgcaaaacaa atccatttcc cgtccggcga ccagcacggc gacgactcat
     2581 cctgcggcga tttggtcaac cgcttcagat tgctgccgtc ggcatcgcag acatacaagt
     2641 ccgtccagcc gtccttgctc aagatcatgg ccaccttgct gccgcggggc gaagccgccg
     2701 ggctcatgtt cgacccgcca aaccgcgcga aagcccggcg cgcgcctgtg gagaggtcat
     2761 gatagaagat gtccgcatgg ttgagtttgt aggaaacgta gtacagcgcc aggtatcccg
     2821 gcacccaact cggcgccgcc acaaggttgt tgtccctggt caccgcctgt gcgttctggc
     2881 catcgaagtc ggcgatgtaa atctcgctgg tcgggctgtt ctcccctttg aacgcaatct
     2941 tcgtctgcgc gatccccttc cggcccagcg ccagaacgaa atcatcagcg aaagcatgcg
     3001 cctgatgccg cagggacgcg ccggagtacg ccttggaaac gagcgtgctc ttattgtggg
     3061 gatcggtggc cttggcctgc aggttgccgt tgttgctgcc gctaatcagg tattgcgccg
     3121 cctcggcgtt cgtgaacgcg aacccctgaa cgtaaaggtc gaaatgcagc gctgccgccg
     3181 cgtcaccgct aatcccgctc atggaaacca agataggcgg cgtattgccg ggtgcaaagt
     3241 tgcctgtgat agttggcagc acctcctggg cgagggccat ggtggtggcc agtagggtga
     3301 atagattaca gatggagagt gtgcgtttca t
//
LOCUS       DATCGS010000254         5762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_518074, whole genome shotgun sequence.
ACCESSION   DATCGS010000254 DATCGS010000000
VERSION     DATCGS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518074"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VIL39_05160"
     CDS             complement(<1..633)
                     /locus_tag="VIL39_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5480849.1"
                     /translation="MATEKYSPPKYYQIRRKIVGSIQRGDLLPGTPVASENEIIERYG
                     VSNTTARKVLHEMEKEGWVTRVKGKGTFVRDFPVVRAINRIFGFTRNMIEAGRKPATK
                     LIGFHLREEDHSQTINGRRFTLKGPFCEIERLRLADGIPMMKETRYISLSLCPDIQRR
                     NLEQSLYEVFEKHYGIQLTEINQMLSAIVLEGEWLTVFQVDRPIPAFRVEG"
     gene            804..3005
                     /locus_tag="VIL39_05165"
     CDS             804..3005
                     /locus_tag="VIL39_05165"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY5480850.1"
                     /translation="MNEPVIVCGRVFRPELVQHLSQMATQQPPPGRNVLAHEVCTHLA
                     WFSPNGRPALSGAKVALRKLSRWGLLSLPLRQGRYMRTHRLQPSGQPLPPVFKVPQRV
                     DQVPGLHLHLITGCEDPWHGLWNDLIIQQHPCGDAPLVGAQLRYLIGSEHGWLGAIGF
                     GPAAFALRARDQWIGWPTGARLSKLNQVVGLARLLIRQEVHCANLASLVLRLVLQRLP
                     SDWQARYGIRPVLVETFVDRSLFTGRTFGAANWQRIGTRSGRGRLGSLPPVKTLKDIW
                     VFPLTSQARRNLQAQAAPVLTPQPVTENLAQTDWCAHELGGLDLGDQRLDRRAQRILE
                     ARWQQPQASFYGSFAGWSPAKAAYDFIEHPQAPISLSRLLEPHTQATQARMAAENVVL
                     LPQDTTTLNYTGLRQTSGLGPLGEESGRGLWLHSLLAFRPDGIPLGLLHAHCWARPPA
                     MPGTDRGRNAKSIDEKESFRWLQAFQAAAAAARRMPQSQLVVLTDREGDLYELHDAVQ
                     IGPPNLHTVIRAQHDRQLECQQKLWAFMAAQPLGKEHPLEVPRRRGQSARLATVEVRW
                     AQIIIHAPAVGCKKGWPPLTLWSVWVCEPHPPQGVEPIEWMLLTDLPVTTWEQAWEKV
                     QWYCRRWGIEEWHRALKNGCGVEQREFKKAEHLQRVLAFDLIIAWRVLACVKLGRALP
                     QLPASVLYSPDELQVLWGTLKKRERPWPAEPDLGPSQSIGGTAGRILGPNL"
     gene            3659..4330
                     /locus_tag="VIL39_05170"
     CDS             3659..4330
                     /locus_tag="VIL39_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480851.1"
                     /translation="MKRWALVVVALYLLILGVLTVPVALLAFAPQASVADIVECYRYL
                     PYWLWMGVMVLGQAALLVVPVRVASRRPITRRSLLWPIVTAGLMMGSLAVGALYSLYE
                     FAVRDKALDGWFGWAGFGSGVLIWGVWAVLFYRSSRTAAPADVISRQCRLMLRGSILE
                     LLIAVPTHIVARSRDYCCAGAMTFVGLTLGISVMLFSYGPAVFFLYAERWRRLHPEPA
                     GESGD"
     gene            complement(4339..4575)
                     /locus_tag="VIL39_05175"
     CDS             complement(4339..4575)
                     /locus_tag="VIL39_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5480852.1"
                     /translation="MPLYEYELCEGSCVVCGGKFTLRRPLSAPPLTKCPACKKPVRRV
                     WSTFNSPKKLSISDAKKAGFTVLKRVNKGEYERQ"
     gene            4715..5335
                     /locus_tag="VIL39_05180"
     CDS             4715..5335
                     /locus_tag="VIL39_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5480853.1"
                     /translation="MTSVLNQHVLVLNRLWQAVNVCTARRALTLLFQGHAQVVLDRND
                     GSFQTFSFSEWHHLSQQEPDPESVHTVSFRIRVPRVILLIAYDRLPKKEVKFTRHNIF
                     ERDQNTCQYCGKVHDRNDLNLDHVIPRDRGGPTTWENIVCSCIECNTHKANRSPQEAG
                     MRLIRKPKRPKWRPFVQISFTLNQHDSWKHFIDLAYWNVELGEDVR"
     gene            5513..5587
                     /locus_tag="VIL39_05185"
     tRNA            5513..5587
                     /locus_tag="VIL39_05185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5546..5548,aa:Glu,seq:ctc)
BASE COUNT         1055 a         1757 c         1774 g         1176 t
ORIGIN      
        1 cccctcgacc cggaaggccg ggatcgggcg gtccacctga aagaccgtca gccattcccc
       61 ctccagcaca atggcgctga gcatctggtt aatctccgtc aactggatgc cgtagtgctt
      121 ctcgaacacc tcgtagagcg actgctccag gttccgccgc tggatgtccg ggcacaggct
      181 caacgagata tagcgcgtct ccttcatcat cgggatgcca tccgccaacc ggaggcgctc
      241 gatctcgcag aagggccctt tcagcgtgaa ccggcgtccg ttgatcgtct gggaatggtc
      301 ctcctcgcgc aggtggaacc cgatgagctt ggtcgccggc tttcgcccgg cttcaatcat
      361 attccgcgtg aagccgaaaa tgcggttaat ggcgcgcacc accgggaaat cgcgcacgaa
      421 cgtccccttg cccttcacgc gggtgaccca cccttccttc tccatctcgt ggagcacctt
      481 gcgggcggtg gtattgctta cgccataccg ctcgatgatc tcgttctccg aggccaccgg
      541 cgtcccgggc agcaggtcgc cccgttgaat cgaaccaacg atcttccggc gaatttggta
      601 atacttcggc ggactgtatt tctcagtggc catggcgttg catcaagctt tccgccgcca
      661 ttaacggttc tggcctctcc cttgtcaagc agcacccaaa gccgcgccca cgtccggccg
      721 catctttgcg gcccgtcccc gctgctcctt ataaccattt atgcgctata aacatttcga
      781 tttccggcgg gcactatcgc gggatgaatg aacctgtaat tgtctgtggc cgagtcttcc
      841 gtcccgagtt ggtgcagcat ctgagccaaa tggcgaccca gcaaccaccg cccggcagga
      901 atgtcttggc gcacgaagtg tgcacccatc tggcttggtt ctcacccaat gggcggcccg
      961 ccctttccgg ggccaaggtg gcgctgcgca agctcagccg ttggggtctg ttgtcactgc
     1021 ctctgcgcca ggggcgctat atgcgcacgc atcggttgca accctccggc cagccgttgc
     1081 cgccagtttt caaggtgccc cagcgtgtgg atcaggtgcc cgggttgcat ttgcatttga
     1141 ttactggctg cgaggatccc tggcatggct tgtggaatga tctgatcatc caacagcacc
     1201 cctgcggcga tgcgccgttg gtgggggccc aattgcgcta cctcatcggg agcgagcacg
     1261 gttggctggg cgccatcggt tttggcccgg cggctttcgc gctccgcgcg cgcgaccagt
     1321 ggattggttg gcccacgggg gcgcgtttga gcaagctcaa ccaggttgtg ggcttggccc
     1381 ggttgctcat tcggcaagaa gttcactgcg ccaatctggc ttcgctggtg ctgaggctag
     1441 tgttgcagcg tctgccctca gattggcaag cccgttacgg cattcgtccg gtgttggtgg
     1501 aaacgttcgt ggatcgttcc ctgtttacgg gacgcacgtt tggggccgcc aactggcagc
     1561 ggatcgggac ccgcagcggg cgggggcgtt tgggctcctt gcccccggtc aaaacgctca
     1621 aagacatctg ggtctttccc ttgacgagcc aggcgcgtcg caaccttcaa gcgcaagcgg
     1681 cgcccgtgct gaccccgcaa ccggtgacgg agaatttggc gcaaaccgac tggtgcgcgc
     1741 acgagttggg cggcctggat ctgggcgatc agcgtctgga ccggcgagcg cagcggattt
     1801 tagaggctcg gtggcagcag ccgcaggcta gcttttatgg cagctttgcc ggctggagcc
     1861 cggccaaagc ggcttacgat tttatcgagc atccgcaagc cccgatcagc ctgagccgct
     1921 tgctggagcc gcacacccag gccacgcagg cgcgcatggc cgccgaaaac gtggtgctct
     1981 tgccccaaga caccaccacg ctcaattaca ccggtctgcg ccaaaccagc ggcttggggc
     2041 cgttgggcga ggagagcggc cgggggttat ggttgcacag cctgctggcc ttccggccgg
     2101 acgggatccc tttggggttg ctccacgccc actgttgggc ccgaccgcca gcgatgccgg
     2161 gcaccgatcg tggccgcaat gccaaatcga ttgacgaaaa agagagtttc cgctggctcc
     2221 aggcctttca agccgccgcg gcggcggcgc ggcgcatgcc ccagagccag ttggtcgtgt
     2281 tgaccgaccg ggaaggggat ctttacgaat tgcacgatgc ggttcaaatc ggtccgccca
     2341 acctgcacac ggtcattcgc gcccagcacg accgccaatt ggaatgtcag cagaagctct
     2401 gggcctttat ggccgcccaa cccctgggaa aagagcatcc cttggaagtg cctcgccgcc
     2461 gaggtcagag cgcgcgcctc gccacggtgg aagtgcgctg ggcgcaaatc attatccacg
     2521 cccccgccgt tgggtgcaaa aagggatggc ccccgctgac gctctggtcg gtatgggtct
     2581 gcgaaccgca tccccctcaa ggggtggagc cgatcgagtg gatgctcttg accgatttgc
     2641 ccgtcacgac ctgggagcag gcctgggaaa aggtgcagtg gtattgccgg cgctggggca
     2701 ttgaagagtg gcaccgcgcg ctcaaaaatg gctgcggggt cgaacaacgc gagtttaaga
     2761 aagccgaaca cttgcagcgg gtcttggcct ttgacctgat tattgcctgg cgggtgttgg
     2821 cctgcgtcaa attggggcgc gccctgcccc agttgccggc cagcgtcttg tactcgccgg
     2881 acgaactgca agtgctttgg ggcacattaa aaaaaagaga gcggccttgg cccgcagaac
     2941 ctgaccttgg cccaagccaa tcgattggtg gcacggctgg gcggatactt gggccgaact
     3001 tgtgacggtg aacccggggc ggaaagtgtc ggcattggct tgcatcggct cgccgatcgg
     3061 ctttggggtt ggcgcctcaa tgcacagaga tctcaatctg ccgaacaacg atgaccaaga
     3121 atgattataa ggagcagcgg ggacgggcgc actccgatac cgtcgtctcc gccgtcactg
     3181 acccattacg aaatgccggc gcgcccgccg cccctcattt tatgcacatc cgactggtgg
     3241 ttcgcgcatc acgtggaccg cgtctaatta tcagggaacg gcatacggat cgggcgcggc
     3301 acaacgccaa acaccgtatg actcccccgc ccggagtcag ccgccagtgg ccacgttgag
     3361 taggaaaaag accccagaac tagaggaaca atagaggaac aatagaggaa taatagagga
     3421 ataatagagg agtagtagag cgcagcaccg ggggcggtgg cttattcctg gcttgattct
     3481 ggccgtgccc cagccatccc gcgcctcgtt cctccaaagc ggcgttacac tggccccgga
     3541 gcagttcccc aacgcgcacc cgcctcctct attgagccca agactggggc ggtggtcaca
     3601 cagtttctgc gaggaaagag atggcgcaat agccttaaac cgaatacaag aacggtgaat
     3661 gaaacgctgg gcgttagtcg tggttgcgtt gtatctgctg atcctggggg tcctcaccgt
     3721 gcccgtggct ctgctggcgt tcgccccgca ggccagtgtg gcggatattg tcgagtgtta
     3781 ccgctacttg ccctattggc tatggatggg agtgatggtc ctcgggcagg cggcgctcct
     3841 agtcgtcccg gtgcgtgtgg ccagccggcg gcccattacc cgccgctcgt tgctgtggcc
     3901 gatcgtgacc gccgggttga tgatgggcag ccttgccgtg ggagcgctgt attccctgta
     3961 tgagttcgcc gtgcgggaca aagcgctgga tggctggttc gggtgggctg gtttcgggtc
     4021 aggggtgctg atctggggcg tctgggcggt gttattctat cgctccagcc gcacggcggc
     4081 gccagcggac gtgatttccc gccagtgccg gctgatgctc cggggtagca tcctggagct
     4141 gctcatcgcc gtgccgacgc acatcgtggc gcgctcccgg gattattgct gcgccggagc
     4201 catgaccttc gtcggcctca cactgggaat ctccgtgatg ctgttctcct atggcccggc
     4261 ggtgttcttc ctgtatgccg agcgctggcg gcgcttgcat ccggaaccag cgggagagtc
     4321 cggggattaa cctccactct actgccgttc gtattcgccc ttgttgaccc gcttaaggac
     4381 ggtaaaccca gccttcttgg catccgagat tgacagcttc ttgggcgagt tgaacgtgga
     4441 ccacacccgg cgaaccggct tcttgcaagc cggacattta gtcaatggcg gagccgaaag
     4501 cggtcggcgc aaggtgaact tcccgccgca caccacgcag cttccttcgc aaagttcgta
     4561 ttcgtataaa ggcataacct tccgtctcta gtccatactc cacattccag actccgaggg
     4621 ctaaagtcaa gggctgtcca gggacggcgg tattcccgtg gcgccgttga caggctgcgg
     4681 cggtctgtgt agtatggtcc cagttggatc ggatatgact tctgtcctca accaacatgt
     4741 gctggtgctt aatcgcctct ggcaggcggt taatgtttgc acggcccgaa gggcgctgac
     4801 acttctgttt cagggccacg ctcaggttgt gctcgaccgc aacgacgggt cgtttcagac
     4861 cttcagtttc agcgaatggc accacttgtc tcagcaggaa ccggacccgg aatccgtcca
     4921 tacggtgtcg ttccgcatcc gggtgccgcg cgtgattctg ctgattgctt acgaccgcct
     4981 gccgaagaag gaagtgaaat tcacccggca caacatcttc gagcgggacc agaacacctg
     5041 ccaatactgc ggtaaggttc acgatcgcaa cgacctgaac ctggaccacg tgattccgcg
     5101 cgaccggggc ggcccgacga cgtgggagaa catcgtgtgc tcctgcatcg agtgcaacac
     5161 gcacaaggcg aaccgctccc cacaggaagc cggcatgcgc cttatccgca agcccaagcg
     5221 accgaagtgg cgtcccttcg tgcagatcag cttcacgcta aaccagcacg acagttggaa
     5281 gcacttcatt gacctcgcct attggaacgt cgagctgggc gaggatgtgc gctaaggcga
     5341 tccgaggccc ggatcccagc ctccggcaag ctcgcattca agtttgggcg gttcccgcga
     5401 cggcctccgg gcgctgaagc gtccagagag ctgctgcttc gcctccgcgc cgcccgcgac
     5461 cttggattga caacgggcca gcaaacgcta gcatttagcg aaatggctgt aaggcgtgtt
     5521 cgtctatcgg ttaggacacg gccctctcaa ggctgaaaga cgggttcgat tcccgtacgc
     5581 gctaccactt tgacggttga gggttgaagg ttgagggcgt aaagccgcgt aaagctgcgc
     5641 cacgcggcca cgccccttta tggggttgag ggattctaaa gttgagtgtt gttataccat
     5701 ttcccgataa taatcggcac atttcccaga cggtagggcc gtgctgctgc gcggccatca
     5761 ta
//
LOCUS       DATCGS010000255         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_172171, whole genome shotgun sequence.
ACCESSION   DATCGS010000255 DATCGS010000000
VERSION     DATCGS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172171"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1307
                     /locus_tag="VIL39_05190"
     CDS             237..1307
                     /locus_tag="VIL39_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY5480854.1"
                     /translation="MPALSLHEEQWEYVRTLLPADLEDSARRTKALVRCRNVPDASAL
                     MRMALAYAVSDLSLKDVAAWASATELAEITGPGLFYRLREADAWLEQMLAQVLGEQLP
                     QAVGGWPVRVVDATVINGPGQKAVQWRAHVLVNPSTGGFRSVELTDDSGGEKLARHPV
                     GKGELVLGDRAYGTARGVYAVRQAEAHVVVRFNPVTLRTCDDQRRRIFLQEREAELPA
                     TGAVEVSVILPIPPERTKSHKTWDSSKAIAWIPVRAIAARTRNAEVIWILTTLTAAEL
                     PALAVLGLYRLRWQIELFFKRLKSLLHLDTLPSREGPTAKSWMLARLIAAALAQRLVQ
                     PSGPLSPWGYQLRELRAEPAQA"
     gene            1631..1858
                     /locus_tag="VIL39_05195"
     CDS             1631..1858
                     /locus_tag="VIL39_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480855.1"
                     /translation="MAGYVAAIKQRGRKTPTLALRQLLRLVREYPREPLLAAVREAAR
                     YGLYDLDRLERMILRRVARDYFLLDGGSHDD"
     gene            1851..2348
                     /locus_tag="VIL39_05200"
     CDS             1851..2348
                     /locus_tag="VIL39_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480856.1"
                     /translation="MTEELEQLLKNLKLRRVLEIYDEQLRAAEKEDVTYSDLVTRLVR
                     AQWHARQEGALEWRIRRANLPERLSARPSPVARRVGIHVITFEACSGFTRVTAYKVAH
                     PPFVGFIAKLRPNRFPGSDARKLPSSTNNLLGWVLPPLVICALGRTRNSRGRACPTRR
                     AEERI"
     gene            complement(2353..>2750)
                     /locus_tag="VIL39_05205"
     CDS             complement(2353..>2750)
                     /locus_tag="VIL39_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480857.1"
                     /translation="ATDFGKPYTYRVRTIVKLAGGKEAESDLSDEIGITPQDVFPPAA
                     PSGLTASAAPNSIELAWNPITEPAPGGYRVYRSTAGGSFEKIADASPIPAYSDRTAEH
                     GKSYRYALTAVSKTGYESPRSELVEITLP"
BASE COUNT          500 a          857 c          862 g          531 t
ORIGIN      
        1 acctgctggc gttccagccg cctccaggcg aagaccacgg ctggcgggcc ctggaagtcc
       61 tgctgcgctc gtccagaggc cgcaaggtgc gcgcccgaga gggatactac ccggattagc
      121 cccaataccg ttccctttgc ggacgcagtc gtggagtggc ctttcaggct ggggggcgcc
      181 ccataggcgt taaaataagg attgatcttc aggcgagttt tctgtgaagc tgtgctatgc
      241 ccgcattgtc tcttcatgag gagcagtggg agtacgtgcg caccctgctc ccagccgatt
      301 tagaggattc agctcgtcgg acgaaagcgt tggtgcggtg tcggaatgtc ccggatgcat
      361 cagcgctgat gcgcatggcg ctggcttatg cagtgtcgga tctttccttg aaagatgtgg
      421 cggcatgggc cagcgccacg gaactagccg agatcaccgg accaggctta ttttatcggc
      481 tgcgcgaggc ggacgcgtgg ttagagcaga tgctggcgca ggtgcttggc gagcaactcc
      541 cacaagcggt tggcggatgg ccagtgcgag tagtggatgc cacggtcatc aacggccccg
      601 gccagaaggc ggtgcaatgg cgcgctcacg tattggtgaa tccatcgacc ggaggatttc
      661 gctcagtgga gttgaccgat gattccggcg gcgagaaact ggcgcgccac ccagtgggga
      721 aaggagaact ggttttagga gaccgggcct atggaacggc gcgtggggta tacgcggtgc
      781 ggcaggcgga agcccatgtg gtggtgcggt tcaatccggt cactctgcgc acgtgcgatg
      841 atcagcgccg acgcatcttc ttgcaggaaa gggaggctga attgcccgct acgggggcag
      901 tggaagtcag cgttatttta cctatacccc cagagcgcac caaaagccac aagacttggg
      961 attcctccaa agccattgcc tggattcccg tccgtgccat cgccgcccgc acgcgcaacg
     1021 cagaagtcat ttggatactc accaccctca ccgctgcaga attgcccgca ctggcagtgc
     1081 tgggattata tcggctgcgt tggcaaattg aactattctt taaacgacta aagtccttac
     1141 tgcacctgga cacactgcct tcgcgcgaag gtcctactgc caagagttgg atgctggccc
     1201 gcttgatcgc cgcagccctg gcacagcgcc tcgtgcagcc atccggtcca ctttccccct
     1261 gggggtacca attgcgagaa ttgcgagccg aacccgccca ggcatagtgc atggtcccgc
     1321 ttccgcatga cactctgggc actccgcgtg gccgttctgg gcgatggtcc ttcggcgctc
     1381 gggagaactt acccacctct gctttccaga aatgacccag gcattctgat ctgagccgcg
     1441 gaccgtccgt gccgccgctt gtgaccatga agcatcacca tgtcagaggc ccggaagaga
     1501 ccgtgtgcga tctgccgccg ttggttcccc gcgagctctt cgcggtggag cggctgcatc
     1561 tgaagccgct gccggcctgg attcccgagg tctacccgaa gagaaggcca ttctggaggc
     1621 cgcgcccgag atggccggct atgtcgcggc gatcaagcaa cgaggccgca agacgcctac
     1681 gcttgccctg cgccaactgc tgcgcctggt gcgcgaatat ccacgggaac cgctgctggc
     1741 ggccgtgcgg gaagccgccc gctacggcct ctacgacctg gaccggctgg aacggatgat
     1801 tctgcgccgc gtggcgcgcg actactttct gctcgacggt ggctcacacg atgactgaag
     1861 aactcgaaca actgctgaag aatctcaagc tccgccgcgt cctggagatc tacgacgaac
     1921 agttacgcgc cgccgaaaag gaagacgtta cctactcgga tctggtcact cgtctggtgc
     1981 gggcgcagtg gcacgcacgg caggaaggcg ccctggagtg gcgcatccgg cgcgccaacc
     2041 tgcccgagcg cctgtccgca cggccttccc cggttgctag gcgggtcggc atccacgtca
     2101 tcactttcga ggcctgctcg ggctttacac gcgttacggc ctacaaggtt gctcacccac
     2161 cattcgtggg ctttatcgcg aagctccgcc ccaaccggtt tcccggctcg gacgctcgca
     2221 agctaccaag ttcaaccaac aacctccttg ggtgggtcct tcccccactg gtgatctgcg
     2281 cccttgggcg cactagaaac tccaggggcc gggcatgtcc gacccgcagg gctgaggaac
     2341 ggatctgaat cgtcacggca gcgtaatttc caccagctcc gagcgcgggc tctcgtaacc
     2401 cgtcttgctt accgctgtca gggcgtagcg gtagctcttg ccgtgctccg ccgtccggtc
     2461 cgagtaagcc ggaatcggac ttgcgtccgc gatcttctcg aaagatccgc cggcggtgga
     2521 gcgatatacg cggtagccgc cgggcgcagg ctcggtgatg gggttccagg ccagctcgat
     2581 ggaattcggc gcggccgagg ctgtcagccc cgatggcgca gcgggcggga acacatcctg
     2641 gggcgtgatg ccgatctcat ccgacaggtc gctttcggct tccttcccgc cggcgagctt
     2701 cacaatcgtc cgcacccgat aggtgtaggg cttgccgaaa tccgtggccg
//
LOCUS       DATCGS010000256         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_716268, whole genome shotgun sequence.
ACCESSION   DATCGS010000256 DATCGS010000000
VERSION     DATCGS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716268"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VIL39_05210"
     CDS             complement(<1..510)
                     /locus_tag="VIL39_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480858.1"
                     /translation="MNTNLPAADLSWKRSRYGFASFWFLSFLAAWFLLRLVLLVVFKP
                     AAVPVTDVLLALVSGLHRDIFAALVETIPLLAWMLIIPDRRFGALWHRVLFIGACLGF
                     WFVQVFLLFVEFFFFDEFKARFNTVAVDYLIYPKEVFVNIWESYHVGVVLAVCLVLSL
                     GWLFAASRLF"
     gene            complement(553..1215)
                     /locus_tag="VIL39_05215"
     CDS             complement(553..1215)
                     /locus_tag="VIL39_05215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5480859.1"
                     /translation="MGTKYVPLNDRLYRYLCRCRSDAADPLLQELRAETAALGKEISN
                     CQISDDQGTFLSILVAAAGVKSAIEVGTFTGYSSLCIARALPPEGKLICLDKSEEWTA
                     VARKYWAKAGVQDRIELRLGAAITLLKQLKRGLTFDFAFIDAAKTEYDAYYELLLPRI
                     RRNGLILFDNMLWGGRLGAGAVKQANGRAIDALNHKLAGDPRVEAVLLSVADGIQLCR
                     KR"
     gene            complement(1317..2264)
                     /gene="fmt"
                     /locus_tag="VIL39_05220"
     CDS             complement(1317..2264)
                     /gene="fmt"
                     /locus_tag="VIL39_05220"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5480860.1"
                     /translation="MAPLRVLFMGTAELSCDSLRGLLESPDFNVVAVVTQPDRPKGRD
                     LKLQPSPVKLLGMSAKLPVLQPERARSEEFLEELRALQPDLIVVAAYGQILPASILDL
                     PRFGCLNVHTSLLPKYRGAAPIQWAILNGDTETGVTIMKMDVGLDTGDILTQATTPIR
                     PEDNSETLHDRLARMGAELLVRTIPDYVAGKLPLQPQSAEGVSYAAKIKKQDGHIDWT
                     QPARAIWNRVRGLAPWPGAFTYLPGQPQPHLLKLWQAEVVERAGPSGEVLQADKTGVV
                     VGCGHEALRILILQREGGRRLQAHEFLAGHPLLPGQKLG"
     gene            complement(2282..2521)
                     /locus_tag="VIL39_05225"
     CDS             complement(2282..2521)
                     /locus_tag="VIL39_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480861.1"
                     /translation="MPQQFGTFTASELYCPKCRCAQPVRERLLLVLPTGELYEFLCSR
                     CATSLGKRTVTGPAVAPAAARRPPPRPARTRRLLR"
     gene            complement(2523..>3282)
                     /locus_tag="VIL39_05230"
     CDS             complement(2523..>3282)
                     /locus_tag="VIL39_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480862.1"
                     /translation="EPLVEPFIEITQEERIKKTEPNIKQPPRLERPAAQSLIGALFSS
                     SLGSFVLLLLYAANIYAGYEVAIFRARPVPLVCGVSAVLPLAGPIIFLSMPTKLAPPE
                     ETWQAAAAAAAETAAPDMVNPMAAEGAEHPTGLKLAGTGPADEAKSGQSEGGEQKPVL
                     PPTTTFQRGQFTFNRRFFETKFPGFFGVIRRDADRDMVLIIRSSRGEYIGQRISRIAA
                     NDLHLQVQRGHASEEVMIPFIEIQEIKLKHKDAK"
BASE COUNT          666 a          988 c         1040 g          588 t
ORIGIN      
        1 aaagaggcgg ctcgccgcaa acagccagcc caggctcagg accaggcaaa ctgcgagcac
       61 aacacccacg tgatacgact cccagatgtt gacgaagact tccttgggat agatgaggta
      121 atcaacggcg accgtgttga agcgcgcttt gaactcatca aagaagaaga actcgacgaa
      181 tagcaggaag acctgcacga accagaagcc aagacaggcg ccaatgaaca acacccgatg
      241 ccacaaggca ccaaaccgtc ggtccggtat gatcagcatc caggccagca gcgggatcgt
      301 ctccaccagg gccgcgaaga tgtcacgatg caacccgctg acgagggcga gcaggacatc
      361 ggtcacaggc acggcggccg gcttgaagac aacgagcaga accagcctga gcaggaacca
      421 ggcggccagg aacgagagaa accaaaagct ggcgaagccg tagcgcgacc gtttccacga
      481 cagatcagca gccggaaggt tagtgttcat tgaatccaat aaaccgtttt caaagagtgg
      541 ccgcaaatcg ttttaccgct tgcggcagag ctgaatgccg tcagcgacag aaagcaaaac
      601 ggcctcgacg cgcgggtcgc cggccagctt gtgattcaac gcatcaatcg cgcgcccgtt
      661 agcctgcttc actgcgccgg cgccaagccg tccgccccag agcatgttgt cgaacagaat
      721 gaggccgttc cgcctgatgc gcggcaggag cagctcgtag taggcgtcat attcggtctt
      781 ggcggcatca ataaacgcga agtcaaaggt tagcccgcgt ttgagttgct tcagcagggt
      841 aatggccgcg cccaggcgga gctcgatccg gtcttgcacc ccggccttcg cccagtactt
      901 ccgcgctact gccgtccact cctcactctt atccaggcaa atgagcttgc cctccggggg
      961 caaggcgcgc gcgatgcaca gggagctgta gccggtgaag gtcccgactt cgatggcgga
     1021 tttcacgcca gcggccgcga ccagaatgct caggaacgtg ccctggtcgt cgctgatctg
     1081 gcaattgctg atttccttgc ccagcgccgc agtctccgcg cgaagttcct gcaacagcgg
     1141 gtccgccgcg tcactgcggc agcggcagag atagcggtat agacggtcgt tcaacggaac
     1201 gtattttgtg cccatgcaga gacagtagca gaattggcgt tggtggaaag ggggatgggt
     1261 gatgcgtgat gcgtgatgcg tgattggcga ccgtggcacc cgagagaagg cagagcttag
     1321 cccagctttt gaccgggcag caacgggtgg ccggcgagga attcgtgggc ttgcaggcgc
     1381 cggccgcctt cgcgctgcag gatcagtatc cgcagagcct catggccaca gcccaccacc
     1441 acgccggtct tgtcggcttg caacacctcg ccggacgggc cggcgcgttc gacgacttct
     1501 gcttgccaaa gtttcagcaa gtgaggttgg ggttggccgg gcaaataggt gaacgccccg
     1561 ggccagggcg ccaaaccgcg cactcgattc caaatagctc gggccggctg ggtccaatca
     1621 atgtgtccat cctgcttctt tatcttggcg gcgtaactga ctccctcggc cgactggggc
     1681 tgcaaaggaa gtttgcccgc gacataatcc ggtatggttc gcacgagcaa ctccgcgccc
     1741 atccgggcga gccgatcgtg cagcgtctcc gagttgtctt ccgggcgaat tggagtggtt
     1801 gcctgcgtga ggatgtcacc ggtgtccagg cccacgtcca tcttcatgat cgtgacgcca
     1861 gtctcggtgt caccgttgag gatggcccac tggatcggcg ccgcgccgcg atacttgggc
     1921 agcagggaag tatggacatt gaggcaaccg aagcgtggca agtccagaat gctcgcgggc
     1981 aggatctggc cataggctgc gactacgatc aggtccggct gcagagcgcg cagttcctca
     2041 aggaattcct cgctccgtgc ccgctcgggc tggagcacgg gcagcttcgc gctcatgcca
     2101 agtaatttga ccggtgacgg ctgcagcttc agatcgcgcc ccttgggacg gtccggctgc
     2161 gtgactaccg ccaccacgtt gaaatccggc gactcaagca gcccacggag actgtcacac
     2221 gacaattctg ccgtgcccat gaagaggact cgtaacggag ccatgcgagg gaagggccgg
     2281 gctaccgcag cagccggcgg gttcgggcgg ggcgcggcgg cggccggcgc gcagcagcgg
     2341 gcgctaccgc cggtccagtc accgtgcgtt tgcccaggga cgtagcgcag cgggaacaga
     2401 ggaattcgta gagttcaccg gtggggagca cgagcaggag ccgttcgcgg actggttggg
     2461 cacaacggca cttcgggcaa tagagttcgc tggcggtaaa ggtgccaaat tgctgcggca
     2521 ttttacttcg cgtctttgtg cttcagcttg atctcctgga tctcgatgaa cgggatcatc
     2581 acctcctcag aggcgtggcc gcgctgtact tgcaggtgga ggtcgttggc ggcgatgcgg
     2641 ctgatccgct ggccgatata ttcgccgcgg gatgatctga tgatcagcac catgtcacgg
     2701 tccgcatcgc ggcgaatgac cccgaagaag ccgggaaact ttgtctcaaa gaaccggcgg
     2761 ttaaaggtga actggccgcg ttggaaagtc gtggtgggcg gcaagacggg cttttgctca
     2821 cccccttcgg attgccccga tttcgcctca tccgcaggtc ccgtgcccgc caacttcaac
     2881 cccgtcgggt gctccgctcc ctctgccgcc atcgggttga ccatgtctgg cgccgcagtc
     2941 tctgctgccg ccgccgccgc tgcctgccag gtttcttcgg gcggcgctaa cttcgtcggc
     3001 atggacagga agatgatcgg gccggcgagc ggcagcacgg cggagactcc gcagaccagc
     3061 ggcaccggtc gcgcgcggaa aatggccacc tcatacccgg catagatatt ggccgcataa
     3121 agcaggagca gcacgaacga ccccaggctg gacgaaaaga gcgctccgat caaagactgg
     3181 gccgccggcc gttccaggcg tggtggttgt ttgatgttgg gctcggtctt cttgattctc
     3241 tcctcctgcg taatctcgat aaacggttcg accagcggct cc
//
LOCUS       DATCGS010000257         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_518676, whole genome shotgun sequence.
ACCESSION   DATCGS010000257 DATCGS010000000
VERSION     DATCGS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518676"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VIL39_05235"
     CDS             <1..700
                     /locus_tag="VIL39_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480863.1"
                     /translation="LVSEIHASPELRDAARTNRTDLLFVEASAHLVQNDLQGTEAAVV
                     ASLRKYPGDQDLLAAATQVYMKYGSYFDNLLAAKAGSDTNTCTYMRYGCYSNALTAVE
                     QQLAIAPTNMTALINKGYVCMQVGAFEQAIPPLTKVLAMDTNNYSALLNRAIAFLRSD
                     KLDAAQRDYGVLQKAFPTAFQIYYGLGEIAWLKKDTNAAIRNYQLYQANAPTNTTEAK
                     LVSARLKELKPGPH"
     gene            710..1870
                     /locus_tag="VIL39_05240"
     CDS             710..1870
                     /locus_tag="VIL39_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5480864.1"
                     /translation="MRVTHVITRLIVGGAQENTIASVLGLREKPGLDVDLVAGPTLGP
                     EGSLESLFADHPAQLTIVPQLVRPLHPWKDYLAWRKLTELFRARQPEIVHTHSGKAGV
                     LGRLAAARAGVPIIVHTIHGPSFGPFQGPLANTLFRSAERYAARVTTHFVVVAEAMKQ
                     QYLAAGIGGSDQYTEIFSGFPLEPFLSSTNDLQLRARFGLSPEDIVIGKIARLFKLKG
                     HQDLLAVAPKLVRSCPRIKFLLVGDGRWRGRLENQVRSLGLERHVVFTGLVAPAAVPP
                     LVGIMDLLVHLSLREGLPRALPQALAAARPVVAYDSDGASEVCLENETGFLLRPGDLP
                     GLQERLLRLAQDAVLRERLGRCGRQFVQQRFGVQRMVDDLHQLYMRLRAFPA"
     gene            1867..2916
                     /locus_tag="VIL39_05245"
     CDS             1867..2916
                     /locus_tag="VIL39_05245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY5480865.1"
                     /translation="MTFPFNVYLLAFVSACVTVLVSLPLWRKWCLRAGLVDDPGYRKI
                     HDRPIPLAGGLAVMTGLLVPTLLAGLVLWWQRAGGDGGGHLVIPGSAARGAGRLALLD
                     SDSAFLLQYGLGRRAIELAGIFLGAFGMLWVGWLDDKHELKPRAKFAGQLLIAGLVAA
                     TGVRITLFVPNIIFSYAITILWILTVINAFNFMDNMNGLCAGLGAIGACHFAIMAAAD
                     GQYLVALIAFLTFGALLGFLPYNFPSARAFLGDAGSHLVGYLLAVLAILPHFYSMRHL
                     QRWAVLIPLLVLAIPLADLVWVVLLRWRIGQPFYEGDTNHLSHRLARRGLTRAQAVLV
                     IWLMAEALAILACLL"
     gene            complement(2953..>4621)
                     /locus_tag="VIL39_05250"
     CDS             complement(2953..>4621)
                     /locus_tag="VIL39_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5480866.1"
                     /translation="YRLRLLVGCGAASGIAAAYNAPISGAVFAALIVLGNFSMSLFAP
                     LVFASVVATMVSRSFFGIRPWYTVPPFEFTSLTQLPWFVCLGIASGVMGALFMKLLNR
                     TEAAFKKVPAPIYIRLALGGLVVGLIAVWYPGVWGNGYVITNRILQGEYGAPSFSAKD
                     IANLESFANKLKRPGQDDEVSKYLAAQLAPTTLNLLSNYTGNLDAKLQQALAANLNRV
                     IRSGPLYEAQRFTNVTLTARTTRLLEREPQGLDLVRLNRKLLLEVYPLEMSRTHWREA
                     AAAGLMLLAGLVVAKLAATLATVGSGAVGGVFTPTLFLGAGLGATFALTLQQLGAGED
                     LPIAAFAVVGMGSMLAATTRSPLLAMIMVFEISLDYSLMPPLMLACVVSILVARRLHP
                     ESIYTEPLRRKGLILPQEATASEAAAERTVGDIMRAPVPPVRETATLREIANRFLTGA
                     NNFLPVVDAKNQLVGLVALQDLKEYLGAEEEFRGVIAYDFMRPPPPCVTPNQRLLDVL
                     PVVLASEQRNIPVVNTLKENRLVGALPRMEVLGIFSEAIAASSKAEA"
BASE COUNT          826 a         1563 c         1302 g          930 t
ORIGIN      
        1 gctcgttagt gagatccacg cttcccccga gctgcgcgat gccgctcgta ccaaccggac
       61 ggatttgctt ttcgtcgaag cctccgctca cctggtccag aacgacctcc aaggcacaga
      121 ggcggccgtg gtggcgagct tacgcaagta tcccggcgac caggatttgc tggctgcggc
      181 gacgcaggtg tatatgaagt atggaagtta ctttgacaac ctgctcgcgg ccaaggctgg
      241 ctcggacact aacacgtgca cgtacatgag gtatggatgt tactccaatg cgcttaccgc
      301 cgtcgaacag cagctcgcca tcgcgccgac caacatgact gcgctcatca acaaaggcta
      361 cgtttgcatg caggtcggcg cctttgagca ggccattccc ccgctcacca aggtgttggc
      421 tatggacacc aacaactact ccgctctgct gaatcgcgcc attgcctttc tgcgtagcga
      481 caaactcgat gccgcccaac gtgattacgg ggttctgcaa aaggcctttc ccaccgcttt
      541 ccagatctac tacggcctgg gggagattgc ctggctgaag aaagacacca atgcggcaat
      601 ccggaactac cagctctacc aagccaacgc tccgaccaat accaccgaag ccaagctcgt
      661 cagcgcgcgc ctcaaagaac ttaagcccgg cccgcactga ccgcaccgga tgcgggtcac
      721 acacgtcatc acgcgcttaa tcgtaggggg cgcccaggaa aacaccatag cgtctgtgct
      781 gggcctgcgc gagaaaccgg gcttggatgt tgatctggtt gccggcccaa cactcgggcc
      841 ggaaggttcg ctggaatcgc ttttcgctga ccatccggcc cagctcacca tcgtgcctca
      901 actggtccgt ccgcttcacc cttggaaaga ctatctggcc tggaggaagc tcaccgaatt
      961 gtttcgcgcg cgtcagcctg agattgttca cacccacagc ggcaaagctg gcgttctggg
     1021 tcggctcgcc gcggcccgcg cgggcgtgcc gatcatcgtg cacacgatcc acgggccatc
     1081 cttcggcccc tttcaaggtc cgttggctaa cacgctgttc cgctcggctg aacgctatgc
     1141 cgcccgggtc acgacccatt tcgtggtcgt ggccgaggcc atgaagcagc agtacctggc
     1201 cgccggtatc gggggctcgg atcagtacac ggagatcttc agcggatttc cccttgaacc
     1261 gttcctttcc tccaccaacg acctccaact tcgcgccagg ttcggccttt cacccgagga
     1321 tatcgtcatc ggcaagatcg cccgtctctt caagctcaag ggacaccagg acctgctggc
     1381 tgtcgcgccc aagcttgtgc gcagttgtcc tcgaatcaag ttcctgctcg tgggcgatgg
     1441 tcgctggcgc gggcgcttgg aaaaccaggt gcgctcgctc ggactcgaaa gacatgtggt
     1501 cttcaccggc cttgtcgctc cggcagccgt cccgccgttg gtgggcatca tggacttgct
     1561 tgtgcatttg tcgttgcgcg agggtttgcc tcgcgcgctg ccgcaggcgc tggccgccgc
     1621 ccggccggtt gtcgcttacg attccgacgg tgccagtgag gtttgcctgg agaatgaaac
     1681 cgggttcttg ttgcggccgg gcgatctacc cggattacag gaacgcctgc tccggctggc
     1741 tcaggatgcg gtgttgcgcg agcgcctggg ccgttgcggt cggcagttcg tgcaacaacg
     1801 cttcggtgtc cagcgcatgg tggacgacct gcaccaactt tacatgcgat tgagagcgtt
     1861 cccggcgtga ccttcccctt taacgtctat ttgttggcct tcgtgagcgc atgcgtcacg
     1921 gtgctggtct ccttgccgct ctggcgcaaa tggtgtctgc gcgccggact ggttgatgat
     1981 ccgggctacc gcaagattca cgaccggcca atccccttgg ccggcggctt agcggtgatg
     2041 acgggcctgc tcgtgccaac gcttctggcc ggcttggtgc tatggtggca gagggcaggg
     2101 ggcgatggcg ggggacatct cgtcattccg ggctcggctg cccgcggcgc tggtcgtctt
     2161 gcgctgctgg attcagactc cgcgtttctg ttgcagtatg gcctgggccg ccgcgcaatc
     2221 gagttggccg gcatcttcct cggcgcgttt ggcatgttgt gggtgggttg gctggacgat
     2281 aagcacgaac tgaagccgcg cgccaagttc gccgggcaat tgcttatcgc cgggttggtc
     2341 gccgcgacgg gtgtgcgcat cacgctcttt gttcccaaca tcatcttcag ctacgccatt
     2401 accatcctgt ggattctcac cgtcatcaac gccttcaact tcatggacaa catgaacggc
     2461 ctatgcgcgg ggttgggcgc gattggggcc tgtcatttcg ccatcatggc cgcggcagac
     2521 ggccaatacc tggtcgcgct gatcgccttc ctgaccttcg gcgccttgct tggcttcctg
     2581 ccctacaatt tcccgagcgc ccgcgccttt ctcggtgacg ccggcagtca tctggtcggt
     2641 tatctgctgg cggtgctggc gattctgccc cacttttact ccatgcggca cttgcagcga
     2701 tgggcagtct tgattcccct attggtcctt gccatcccgc tggcggactt ggtttgggtc
     2761 gttcttctgc gctggcgcat tggccagccg ttctatgaag gcgataccaa ccatctgtcc
     2821 caccggttgg cccggcgagg ccttacccgc gcgcaggccg tgctggtcat ctggcttatg
     2881 gcggaagcac tcgccatcct ggcatgcctg ctctgagcca atagccagtg gccaattggc
     2941 caatcgcagc cgctacgctt ccgccttgct gctcgcggca atcgcttctg agaagatccc
     3001 cagcacttcc atgcgcggca gtgccccgac cagccggttc tccttcagcg tattgaccac
     3061 cgggatattg cgctgttcgc tcgccagcac caccggcagc acgtccagca aacgctgatt
     3121 cggcgtcacg cacggcggcg gcggccgcat gaagtcgtaa gcaatcaccc ctcggaattc
     3181 ctcctccgct cccaggtatt ccttcaaatc ctgcaaggcc accaggccca ccaactggtt
     3241 cttcgcatcc actaccggca ggaagttgtt cgcgccggtc aggaaccggt tggcaatctc
     3301 ccgcagcgtc gccgtctcgc gcaccggcgg caccggcgct cgcatgatat ccccgaccgt
     3361 tcgctcggcc gcggcttccg acgccgtggc ctcctgcggc aagatcaatc ccttgcgccg
     3421 cagcggctcc gtgtagattg attcgggatg cagccggcgc gccaccagga tggacaccac
     3481 gcacgccagc atcagcggcg gcatcagcga ataatccaat gagatctcga agaccataat
     3541 catcgccagc aatggtgagc gcgtggtcgc cgccagcatg ctccccatgc ccaccaccgc
     3601 aaacgccgca atcggcaagt cctctcccgc gcccaattgc tgcaaggtca aagcaaacgt
     3661 cgcccccaat cccgcgccca ggaacagcgt cggcgtgaac accccgccta ccgcccccga
     3721 gccaaccgtg gccagcgtcg ctgccagctt cgccaccacc agtcctgcca gcaacatcaa
     3781 ccccgccgct gccgcctcgc gccagtgcgt ccgggacatc tccaacggat aaacctccaa
     3841 cagcagcttc cgatttagcc gcaccaggtc cagtccttgc ggctctcgtt ccaacagccg
     3901 tgtcgtcctc gccgtcaacg tgacgtttgt gaatcgctgc gcttcgtaaa gcggtccgct
     3961 ccgaatgacc cgattcaggt tggctgccag cgcctgctgc aacttcgcgt ccaggttgcc
     4021 ggtataattt gacaataggt tcagcgtggt cggcgcgagc tgcgccgcga gatacttaga
     4081 gacttcgtca tcctggcccg ggcgcttcaa cttattggca aacgattcca ggttcgcaat
     4141 gtccttggcg gagaacgacg gcgccccata ctccccctgc aaaatgcggt ttgtaatgac
     4201 gtagccgttg ccccacaccc cgggatacca taccgcgatc agcccgacca ccagcccacc
     4261 cagcgccagc cggatgtaaa tcggcgcggg caccttcttg aacgccgctt ccgtccggtt
     4321 caacagcttc atgaagagcg ctcccattac gccgctcgcg atccccagac acacaaacca
     4381 cggcagttgc gtcaggctgg taaactcgaa cggcggcacc gtgtaccatg gccggatgcc
     4441 aaagaaactc cgcgacacca tcgtcgccac caccgatgcg aacaccagcg gcgcaaacag
     4501 gctcatcgag aaattaccca gcacgatcag cgccgcaaac accgcaccgg agatcggggc
     4561 attatacgct gccgcaatcc ccgaggccgc cccgcacccg accagcagcc ttagccgata
     4621 g
//
LOCUS       DATCGS010000258         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_667543, whole genome shotgun sequence.
ACCESSION   DATCGS010000258 DATCGS010000000
VERSION     DATCGS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667543"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VIL39_05255"
     CDS             <1..497
                     /locus_tag="VIL39_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480867.1"
                     /translation="DDYITEEAKGTASLPATLSPPFTVVATNLSLVSGSWANYMNECS
                     YALDPGQFSAWRLAVGLLPCSSTPQVLLIVAGPYLPEETWTGCDNLSLPYPWLGLAYD
                     PAFLCAPSGANGGGGIFRFPLLDGQADLVNQTVSGDSQAGSCSDPSQHATGTIQFHLV
                     HTPK"
     gene            681..1205
                     /locus_tag="VIL39_05260"
     CDS             681..1205
                     /locus_tag="VIL39_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5480868.1"
                     /translation="MNPLKCFGSTRAPASVLLIRVMVGVVFLTEGVQKFLYPEELGAG
                     RFIKIGIPAPEVMGPFVGVVEIVCGVLLLLGLLTRLAAVPLLINISTAIVSTKVPMLL
                     GHGFWTFTLAKLPRYGFLSMLHEARTDFCMLLGLLFLLIVGAGAWSVDVVLSRRLMNG
                     EAAKTDAPTIGGRR"
     gene            complement(1259..2170)
                     /locus_tag="VIL39_05265"
     CDS             complement(1259..2170)
                     /locus_tag="VIL39_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY5480869.1"
                     /translation="MKTTWQSFLLFSISALAAVSACAASAASAAPATASAPGSKWELV
                     WSDEFDRDGLPDPAKWNYEEGFIRNQEKQYYTKERKENARVENGMLVIEGRKEQFKNP
                     RYRAGSANSRSADEYAEYTAASLITSRKASWLYGRIEVRAKLPQGQGVWPAIWMLGDN
                     HNTAGWPRCGEIDIMEFVGHDPNHVHATVHYSLDRKHKSSGSQLQTPAPYNDFHIYAV
                     EWNKDRMDFFFDDKKYHTFELKAADENGENPFRKPQFLMVNLALGGAWGGKIDDAIMP
                     QKFLIDYVRVFKEKDGGQESKAPANQP"
BASE COUNT          425 a          738 c          774 g          552 t
ORIGIN      
        1 cggatgacta catcaccgaa gaggccaaag ggaccgcgag cctcccagcc actctatccc
       61 cgcctttcac agtcgttgcc accaacctca gcctcgtgtc gggcagttgg gcgaactaca
      121 tgaacgaatg ctcctatgct ctggacccag ggcagttcag cgcctggcgc ctggcagtgg
      181 ggctgctgcc ctgcagttcg accccgcagg tgctcctaat agttgccggc ccgtacctgc
      241 ccgaagaaac ctggacgggt tgcgacaacc tctctctgcc atacccgtgg ctgggtcttg
      301 cttacgatcc cgccttcctc tgtgcgccca gcggggccaa tggcggcgga gggatattcc
      361 gcttcccctt gctggacggc caggccgatt tagttaacca gactgtgtcg ggtgatagtc
      421 aggccggctc atgctctgat ccgtcccagc atgccaccgg gaccattcaa ttccacctgg
      481 tgcatacccc gaaataggca atccggacaa cgccgatggg gcggaaaacc cgagccgctc
      541 gcaggcggcg cgaccccggg caatgaacca ggcgacgatt acgcggcaga gccgttgcgg
      601 ggcggcggga tgggcatgcg gggcggggca gtttgacaaa agaggggggt gaattacggt
      661 tattgaactc ggttaccgca atgaatccac tcaaatgttt cggttccact cgggcaccgg
      721 cgtcggtgct gctcattcgg gtgatggtcg gcgtggtgtt tttgaccgag ggcgttcaga
      781 agttccttta tcccgaggaa ctgggggccg ggcgtttcat caagatcggg attcctgcgc
      841 cggaggttat ggggccgttt gtcggagtgg ttgaaatcgt atgcggggtg ctgttgctcc
      901 tcgggttgct gacgcggctg gcggcggttc ctctccttat caacatctcg acggcgattg
      961 tttccacgaa agtgccgatg ctgctcgggc acgggttttg gacgttcacc ctagccaagc
     1021 tgccgcgcta cggttttctg agcatgctgc acgaggcgcg cacggatttt tgcatgctgc
     1081 tgggcctgct gttcctgctg atcgtcggcg cgggcgcgtg gtcggtggat gtcgtgctct
     1141 cgcgcagact gatgaacggt gaggcagcca aaacagacgc gccaacaatc ggaggacgaa
     1201 gatgaacgag gggagttgaa gcgttttccc atgatgcacg aagcggagtc aggcccgttc
     1261 agggctggtt tgctggcgcc tttgattcct ggccaccgtc tttctccttg aagaccctca
     1321 cgtagtctat caggaacttc tggggcatga tcgcgtcgtc aatcttgccg ccccaggccc
     1381 cgccgagcgc gagattcacc atcaggaatt gcggcttgcg gaaggggttc tcgccgttct
     1441 cgtccgcggc cttcaactcg aaggtgtgat acttcttgtc gtcgaagaag aaatccatgc
     1501 ggtccttgtt ccattccacc gcgtagatgt ggaagtcgtt atagggagcg ggcgtctgca
     1561 actggctgcc gctggatttg tgcttccggt cgaggctgta atgcaccgtc gcatgaacgt
     1621 gattggggtc gtggccgacg aactccatga tgtcaatctc gccgcaacga ggccagccgg
     1681 cggtgttgtg gttgtcgccc agcatccaga tcgcgggcca gacgccctgg ccctgcggca
     1741 gcttcgcgcg cacctcgatg cggccataga gccaactagc cttcctggac gtgatcaggc
     1801 tcgccgccgt gtactcggca tattcgtccg cgctccggga attcgccgag ccggccctgt
     1861 acctcgggtt cttgaactgc tccttgcgac cctcgatgac caacatcccg ttttcgacgc
     1921 gtgcgttctc tttgcgctct ttcgtatagt attgcttctc ctggttgcgg atgaatcctt
     1981 cctcgtagtt ccatttcgcg ggatcgggca gcccgtcgcg gtcgaactcg tcggaccaga
     2041 ccagctccca cttcgagccg ggcgcagagg cagttgccgg cgcggcggaa gctgcgctcg
     2101 ccgcgcaggc ggaaaccgcc gccagcgcgc tgatcgagaa tagaaggaat gactgccagg
     2161 ttgtcttcat gattgaacgc gctcctttgg gaatatcgct gatttttcgg tctcacgcat
     2221 gaggccctga tgctgccaaa cgggagccgt gatgtcaaac tggagtttcg ccgcctttct
     2281 catcgtgcac tccagtttca gggtttcggc ggcgcgggcg cgggcggggg cgatggtgcg
     2341 tgccgccgcc caacgcgcac ataacagcgg tggattagtg tcaatagtga gacacttgct
     2401 cggccacacc ctcaagccgt cttcaaggca cctgcgagcc ggaagctctg gaggtgtctt
     2461 cgtattcgct tcgtattcgc ttcgtattc
//
LOCUS       DATCGS010000259         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_667578, whole genome shotgun sequence.
ACCESSION   DATCGS010000259 DATCGS010000000
VERSION     DATCGS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667578"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VIL39_05270"
     CDS             <1..1495
                     /locus_tag="VIL39_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY5480870.1"
                     /translation="NTKLLISAAFVLLAGGCTVGPNYQRPNVKLPAQWGEPLASGVTN
                     HDNLVGAWWKKFHDPELDSLIGRAVTTNLDLRIAEARVRQARAQQGFAEAGYWPTVNA
                     SGAYARQKQSENQPIIGSIPSSSNIPFENSVYQAGFDAAWEIDLFGGKRRTVAAATAA
                     LAAVEYGRRDVLVTLLSEVARNYVLTRGAQRQLAIAQNQIKAQGETVAITQSRLDHGA
                     GTDVDLQRALALLATVQSQVPPIETSIQSSVHRLGVLLAQTPDALQEELSVTAPIPAP
                     PLEVPVGLPSDLLLRRPDVLRAERQLASETARIGQVKADLFPKFFLTGSAGLASISTS
                     DFFSPTSRLWSIGPTVQWRVFDAGRVRASLRAQTAIQDQASLTYEQVVLTSLEDVENA
                     LVAYAKEQEHRHLLERAVTANAKAVDLANQLYTKGLGKYLSLLDAQRALYASQDELVR
                     SDRAVTVNLIALYKALGGGWETDQSQPLAATGLGKPDAALEQARQSP"
     gene            1607..>2323
                     /locus_tag="VIL39_05275"
     CDS             1607..>2323
                     /locus_tag="VIL39_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5480871.1"
                     /translation="MNTCLQGQVVLVTGASGGIGSAIARQFAAEGARVVLHCRAGRAR
                     VRALQKELGAAESLVVAADLSKEAEARRLFAQALQRFGRVDTLVANAGAWETRDVGLH
                     QMSLRQWRRTLDGVLTTTFLTVREFLRLVAKQKRGNAILIGSTAAVFGEAGHADYSAG
                     KAAIAFGLTRSLKNEIVRLAPHTRDYCGGRVNCICPGWTVVPRLAAKLGNATSVRKVT
                     ATMALPQLARPEDMANAAIFL"
BASE COUNT          420 a          774 c          695 g          434 t
ORIGIN      
        1 gaatacgaaa ctccttatct ccgctgcgtt cgtgttgctg gccggcggat gcaccgtcgg
       61 cccaaactat cagcggccga acgtcaagct gccggcgcaa tggggtgaac cgctcgccag
      121 cggcgtcacc aaccatgaca acctcgtcgg cgcgtggtgg aaaaagttcc atgaccccga
      181 gctggattcc ctcatcggac gggccgtgac gaccaacctc gacctgcgca tcgccgaagc
      241 acgcgtgcgg caggcccgtg cccagcaggg ttttgccgaa gccggctatt ggccgacggt
      301 caatgcctcc ggcgcctacg cccgacaaaa acaaagcgag aatcagccga tcatcggttc
      361 cattccctcg agctccaaca tcccgttcga aaacagtgtc tatcaggccg gattcgatgc
      421 cgcttgggag atcgacttgt ttggcggcaa acgccgcacg gtcgccgccg ccactgccgc
      481 gctggccgcc gtggaatatg gccgccgcga cgtactggtg acgttgctat ccgaggtggc
      541 gcgtaattac gtgctgacgc gcggagccca gcggcagttg gccattgccc agaatcagat
      601 taaagcccaa ggagagaccg tagccatcac ccagagccgc ctggaccatg gcgccggcac
      661 ggatgtggac ctgcagcgcg ccctcgcgct gctcgcgact gttcaatcgc aagtgccccc
      721 aattgaaacc tcgatccagt cctccgttca tcgccttggg gttctcctgg cccagacccc
      781 cgatgcgctg caagaagaat tgtcggtcac ggcccccatt cccgcaccgc cgcttgaagt
      841 cccggtcggg ctgccctcgg acctgttgct tcgccgaccg gatgtgctgc gggccgagcg
      901 tcaacttgcg tcggaaacgg cccgcatcgg ccaggtgaaa gccgacctgt tcccgaagtt
      961 cttcctcacc gggtcggccg gcctcgccag catcagcacc agcgacttct tttctcccac
     1021 cagccgattg tggtccatcg gcccgaccgt gcagtggcgc gtttttgacg ctggccgagt
     1081 ccgtgccagc ctccgagccc agaccgcgat tcaggatcag gcgtcgctga cctacgagca
     1141 ggtcgtcctg acctcattgg aagacgttga gaacgcgctg gtcgcctacg cgaaagagca
     1201 agagcaccgc cacctgctgg agcgggcggt taccgcaaac gccaaggccg tggatctcgc
     1261 taatcaactc tacaccaagg gtctgggtaa atacctcagc ctgctcgacg cgcagcgcgc
     1321 cttgtatgcg tcccaggatg agctggtgcg gagtgatcgt gccgtcaccg tgaatctgat
     1381 cgccctctac aaggccctcg gcggcggatg ggaaacggat caatctcaac ccctggccgc
     1441 aacgggcttg ggcaagccag atgccgcgct agagcaggcc cgtcaatcgc cctgacggtc
     1501 ctggtcaggg cggtctcgac catcaacttg cagctatcaa ctcacaaccc tcacctttcc
     1561 agattctctt ggccttcgcg cctgctttga ctaacagtta gccgcaatga atacgtgtct
     1621 gcagggacag gttgttttgg tcaccggggc ttccgggggt atcggctcag ccatcgccag
     1681 gcagtttgcg gccgaaggcg cccgggtggt acttcattgc cgcgccggac gtgcccgggt
     1741 ccgcgcgctc caaaaagagc tcggcgcggc cgaatcgcta gtggtggccg ccgatttgtc
     1801 gaaggaagct gaagcgcgac gcctgttcgc gcaggcgctc cagcgcttcg gccgtgtgga
     1861 cacgctggtg gccaatgccg gggcctggga aacgcgcgat gtgggcctgc accaaatgtc
     1921 gctgcgccaa tggcgtcgaa ccctcgatgg cgtgttgacg accacgtttc tgaccgtgcg
     1981 cgagttcctg cgcctggtgg caaaacaaaa gcgaggcaac gccattctta ttggttcgac
     2041 cgctgcggtt ttcggcgagg ccggccacgc tgactattcc gccggcaagg ccgccattgc
     2101 ctttggtctg acccgttccc tgaagaatga aatagtgcgc ctcgcgcccc atacccgcga
     2161 ttattgcggc gggcgcgtga actgcatctg ccctggctgg acggttgtgc cccgcttggc
     2221 cgctaagctg ggcaatgcta cctccgtgcg caaagtcacc gctaccatgg cgctgccgca
     2281 gctcgcgcgc cctgaagaca tggctaacgc cgcgatcttt ctc
//
LOCUS       DATCGS010000260         4533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_21602, whole genome shotgun sequence.
ACCESSION   DATCGS010000260 DATCGS010000000
VERSION     DATCGS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21602"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VIL39_05280"
     CDS             complement(<1..272)
                     /locus_tag="VIL39_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480872.1"
                     /translation="MRSWRTIFVSAIATLWLAASFHCRLEVLTIFEFLSCCQHSESEQ
                     SPAHHEKECNDDGCAAVERGFYKPARLENAPLIPLLGLAAWLSPLP"
     gene            792..1721
                     /gene="fabD"
                     /locus_tag="VIL39_05285"
     CDS             792..1721
                     /gene="fabD"
                     /locus_tag="VIL39_05285"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5480873.1"
                     /translation="MSKTALLFAGQGAQVVGMGRDLADHSPTAKAWFDRANAALGYDL
                     ASICFNGPEAELTKTEHAQPGIFLVSWAAFELLKERVPALSFQATAGLSLGEFTALTA
                     AGAMSFEEGLKVVRQRGRFMQEACEATRGGMAAIIGLDEAPMREVCAQAGVALANLNC
                     PGQIVISGATDKIEPACELAKAKGAKRALPLTVAGAYHSPLMASAQPKLEAELARVSL
                     VLPAVPVIANVTAQPHDGPDAIRARLVEQVTSSVRWEESMRYLIAQGFTRFIELGPGR
                     ALSGFMKRIDKTVQILNVEDLATLEATAKALAS"
     gene            1778..2521
                     /gene="fabG"
                     /locus_tag="VIL39_05290"
     CDS             1778..2521
                     /gene="fabG"
                     /locus_tag="VIL39_05290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY5480874.1"
                     /translation="MNELTNQVAVVTGAGRGIGQAIALKLAAAGADIACVDLKLEFCA
                     ETVQKTQALGRKAWPFAANVSDAASVEAAAEQILAATGKVDILVNNAGITKDGLLMRM
                     SEADWDAVLDINLKGTFLFTKAFSRSLLKQRSGRIVNIASVVGLIGNAGQCNYGASKA
                     GVIGFTKSAARELASRGITVNAIAPGFIETAMTAKLGPEAREALSKQIPLGSLGQPDD
                     VANAVLFLASPAARYITGQVLSVDGGMAM"
     gene            2598..2843
                     /gene="acpP"
                     /locus_tag="VIL39_05295"
     CDS             2598..2843
                     /gene="acpP"
                     /locus_tag="VIL39_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5480875.1"
                     /translation="MADKTIEQRIKDIIVEQLGVNADQVTPDAKFIEDLGADSLDTVE
                     LVMALEEEFGNEIPDEQAEKLQSVGDVIKYIEDLQQK"
     gene            2909..4168
                     /gene="fabF"
                     /locus_tag="VIL39_05300"
     CDS             2909..4168
                     /gene="fabF"
                     /locus_tag="VIL39_05300"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5480876.1"
                     /translation="MGSNWTDRRVVITGLGVVTPLGHQVDTFWANLIGGQCGISKITR
                     FDASQFDSRIAAEVKDFDPLPALPTPKEMRRTDRFSQFAVYAGWQALRDSGLDLDRVN
                     RDEVGVFIGSGIGGLQTVSEQCKVLLERGPSRLSAFMIPMLISNMASGLFSIYHKLRG
                     PNFATCSACATSNHALGEAWRTIKMGDAQVMFAGGAEATIVPVGIGGFCAMKALSTRN
                     DDPGHASRPFDKERDGFVMGEGAGMLVLEELEHAKRRGARIYCEVVGYGNTADANHVT
                     SPAPGGEGAARCMRMALRHAGLNPEAISYINAHGTSTSQGDIAETQAVKSVFGEHARK
                     LAVSSTKGATGHMLGAAGAVEMVVCTKAIETGIVPPTINYQIPDPECDLDYVPNTARQ
                     MTVNAVLNNSFGFGGHNACILAKKFVG"
     gene            4323..>4533
                     /locus_tag="VIL39_05305"
     CDS             4323..>4533
                     /locus_tag="VIL39_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480877.1"
                     /translation="MAEVVPKLPPEGKPARISYWFMAGTLIMVGGFHLATPLLVAFFA
                     YLALTKLHFVKRGGKWLAVALFVLLV"
BASE COUNT          883 a         1351 c         1423 g          876 t
ORIGIN      
        1 tgaggcaagg gagacaacca ggctgcgagc cccagcaaag gtatcaatgg cgcgttttcc
       61 agccgcgccg gcttgtagaa gccgcgttcg acggcggcgc aaccgtcatc gttgcactcc
      121 ttttcgtggt gggcgggaga ctgctcgctt tccgaatgct ggcaacagga gagaaactcg
      181 aaaatcgtca gcacttccag gcggcaatgg aaactcgccg ccagccagag ggtggctatc
      241 gcgcttacaa agattgttcg ccaggatcgc acttgaccca atagaaaggc aaaagcgcca
      301 ggcgttcaat cttttactgt ctttgctttc ctccgggatc tggaatgccg ccccaagatg
      361 cagcctgttt ggccacgccg ggaagccgcg ctgttgggag cgtgtcttaa gcgtgttgcg
      421 agcggggggt gacggtgttg ctccggtgtt gcgagcgtgt tggtcccttg tatatcgcta
      481 gtacaaccct tgtatacccc ttatctatcc cttgtacatc ccatctgcac ccgtggggac
      541 ttcggcagct ccacggtgtc tccaccgagc cctggcttgg ttttctcaga tatgtgcgct
      601 tgggctggcg ctttggatac agcgggaggc cgcttggcag tggaaacccc gctcgtccac
      661 aggtctagtt gtagggtgaa agaagttaca tggtcacatg gttatctggg ttacatccga
      721 atcaggctgc cctttccttg catatctctg ctttcctcca ggcgaacttt cgtgtttagt
      781 cacgcccgat tatgagtaag acggcactat tatttgcagg gcaaggcgcc caggtggtgg
      841 gcatgggcag agatctggcg gaccattctc ccaccgccaa ggcttggttc gaccgcgcca
      901 atgcggcgct cggctacgat cttgcctcga tctgtttcaa cggaccggaa gcggagctga
      961 cgaagacgga gcatgcgcaa ccgggtatct tcctggtgag ctgggccgcc ttcgagctgc
     1021 ttaaggagcg cgtgcccgct ttgagcttcc aggccacggc gggcttgtcg ctgggggagt
     1081 tcacggcgct gacggcggcg ggtgcaatga gtttcgaaga ggggttaaaa gtagtgcgcc
     1141 agcgcgggcg cttcatgcag gaagcgtgcg aagccacccg cggcggcatg gcggcaatta
     1201 tcggattgga cgaggcgccg atgcgcgagg tctgcgccca agccggcgtg gcgctcgcca
     1261 acctgaattg ccccggccaa atcgtgattt cgggcgcgac cgataagatt gaaccggcct
     1321 gcgaactggc gaaagccaaa ggcgccaaac gcgcgctgcc gctgaccgta gccggggcgt
     1381 atcattcgcc gctcatggcc agcgcccagc ccaagctcga ggccgaactg gcgcgcgtga
     1441 gcctcgtttt gccggcggtc ccggtgatcg ccaatgtgac cgcgcagccg cacgatggcc
     1501 ctgacgccat tcgcgcgcga ctggtcgagc aagtgacctc ctcggtgcgc tgggaggaat
     1561 ccatgcgtta tctgatcgcg caagggttca cgcgcttcat cgagctcggg ccgggccggg
     1621 ctttgagcgg ctttatgaag cgcatagaca agaccgtgca gatcctcaac gtcgaggacc
     1681 tggcgacgtt ggaagcgacg gccaaagcgc tggcgagctg aatccgcaaa acgcgaattg
     1741 acaacagccc cgggatagcg cacaagaagc gcggtttatg aatgaattga ccaatcaagt
     1801 ggcggtagta accggcgccg gccgcggcat cggccaagcc atcgccttga aactcgccgc
     1861 cgccggcgcg gacattgcct gcgtggattt gaagctggag ttttgcgccg agaccgtgca
     1921 aaagacgcag gcgctcgggc gcaaagcctg gcccttcgct gccaacgtct ccgacgccgc
     1981 cagcgtggag gctgccgcgg agcagatcct ggccgccacc ggcaaggtgg acatcctggt
     2041 caacaatgcc ggcatcacca aggacggctt gctcatgcgc atgagcgagg cggactggga
     2101 tgccgtgctg gacatcaacc tcaagggcac gtttctcttc accaaggcat tcagccgcag
     2161 cctgctcaag cagcgctcgg ggcgcatcgt taacattgct tcggtggtcg gcctcatagg
     2221 caacgccggc cagtgcaatt acggggccag caaggcgggt gtgattggct tcaccaagtc
     2281 cgccgcccgg gagctggcca gccgtggcat caccgtgaac gcgattgccc ccggtttcat
     2341 cgagaccgcc atgacggcca agcttggtcc cgaggcgcgc gaggcactct cgaaacagat
     2401 tcccctcggt agccttggcc agcctgacga cgtggccaac gccgttctgt tcctcgccag
     2461 cccggcggcg cgctacatca ccggccaggt gctgtctgtt gacggcggca tggccatgta
     2521 acttttttcg attccgggct tgacgctttc tgaatcgtgg catagacaca ctgcaaaaat
     2581 aagaactgga gtacaccatg gctgacaaaa caattgagca aagaattaaa gatattatcg
     2641 tcgaacagtt gggcgtgaac gcggaccagg tcacacccga cgccaagttc attgaagacc
     2701 tgggagcgga ttcactcgac accgtggaat tggtcatggc gctggaagag gaattcggca
     2761 acgagattcc cgacgagcag gcggagaagc tccaaagcgt gggcgacgtg atcaaataca
     2821 tcgaggacct gcagcaaaag tagtcgtgag ttgaacattt ccggggcggc ttggactgtc
     2881 gtcagcttga gctgccccgt ttgattttat gggcagcaat tggactgacc gccgagtagt
     2941 cattaccggc cttggcgtcg tgactccttt gggtcaccag gttgatacct tctgggccaa
     3001 cctgatcggc ggccagtgcg gcatcagcaa gatcacccgc tttgatgctt cgcagttcga
     3061 ctcccggatt gccgccgaag tgaaggattt tgaccccctt cccgcgttgc cgacgcccaa
     3121 ggaaatgcgg cgtaccgacc gtttttcgca attcgccgtg tacgcgggct ggcaggcttt
     3181 gcgcgattcg ggtctggatc tcgaccgggt gaatcgcgat gaggtcggcg tgttcatcgg
     3241 ctccggcatc ggcgggttgc agaccgtctc cgagcaatgc aaagttctgc tcgagcgcgg
     3301 gccgagccgg ctgtcggcgt tcatgatccc gatgctcatc tcgaacatgg cctcgggact
     3361 cttctcgata tatcacaagc tgcggggccc caattttgcg acctgctcgg cttgcgccac
     3421 ctccaaccac gccctgggcg aagcctggcg caccatcaag atgggggatg cgcaggtcat
     3481 gttcgccggc ggggcggaag ccacgattgt gcccgtcggc atcggcgggt tctgcgccat
     3541 gaaagcgctg agcacccgca acgacgatcc cgggcatgcc tcaaggccgt tcgacaaaga
     3601 gcgggatggg tttgtgatgg gcgagggtgc cggcatgctg gtgcttgaag agttggaaca
     3661 tgcgaaaagg cgcggcgcga gaatctattg tgaggtcgtc ggctacggca acaccgccga
     3721 tgccaatcac gtgacctcgc cggccccggg aggcgaaggc gccgcccgct gcatgagaat
     3781 ggcgctgcgc cacgctggtc tcaaccccga ggccatctcc tacatcaatg cccacggcac
     3841 ctccacgtcc cagggggaca tcgcggagac gcaagcggtc aaatctgttt tcggcgagca
     3901 cgctcgcaaa ctggccgtca gctccaccaa aggcgcgacc ggccacatgc tgggcgcggc
     3961 gggcgccgtg gaaatggtcg tctgcaccaa ggcgattgaa accgggatag tgcccccgac
     4021 gattaactac cagatccccg accccgagtg cgatctggat tacgtcccca acaccgcccg
     4081 ccagatgacc gtcaacgccg tcctcaacaa ctctttcggt ttcggcggcc acaacgcctg
     4141 catcctggcc aagaagttcg tgggctaaga aaggagcgcg gcactggcaa cagggccagt
     4201 gacaaagtgt agcgcatggc gtcctcgcct gataggtttt gacagcaatc gtgcctgggc
     4261 tactcaggtg accccgaaaa aggctggctc cggccgcctc gccgggatat aaacgcgggc
     4321 aaatggcaga agtcgttccc aagctaccgc ccgaaggcaa gcccgcccgt atctcgtact
     4381 ggttcatggc ggggacgctg attatggtgg ggggctttca cctggcgacg ccgctgctcg
     4441 tggcgttctt cgcgtacctg gcgctgacca agctgcactt cgtgaagcgc gggggcaagt
     4501 ggctcgcggt cgcgctcttt gtgcttctgg tgt
//
LOCUS       DATCGS010000261         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_667639, whole genome shotgun sequence.
ACCESSION   DATCGS010000261 DATCGS010000000
VERSION     DATCGS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667639"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..904)
                     /locus_tag="VIL39_05310"
     CDS             complement(113..904)
                     /locus_tag="VIL39_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEY5480878.1"
                     /translation="MICAGIDAGSRATKVVLFDSEQSRIVGSSLADQGVEQQRLASEL
                     LERACQSAQVDRAAVKAIVATGYGRNAIRFADTTITEITCHAQGVHHLAPEVHTIVEI
                     GGQDSKFITLEDGGRVRDFAMNDRCAAGSGRFLEVVATRLGVDLSTLGDLSRHSHQAA
                     LISSMCVVFAETEIIGLLAEGVAPADIAAGVQNAIAQRVVTLAGRTVTPPVCFTGGVA
                     LQSGMVRALETALSCAVCVSSLPQYTGALGAAILASRQSRPGPLL"
     gene            complement(1073..>2562)
                     /locus_tag="VIL39_05315"
     CDS             complement(1073..>2562)
                     /locus_tag="VIL39_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY5480879.1"
                     /translation="FPRGRACELRAGPPSAIRISAFGFPSTFGLRVSDFPMTSPPRKL
                     TLDDWDARYEQLTRAGLREPGYGGPLRRHVVLEGDLRLRHLRFDNSAAALRLWNFLLT
                     ENQRLHAARAQGEKIIGAMKDLGTVPVMAYSLPGLRAFYPDGAWWTPCLMECSDGLLQ
                     QAEQLGIDASFCPVRAMLPAFQNGEHFPRPDALICSTGAVCDDFSAIAQRLEKMSFPI
                     VWWEMPRRRAPDPGEAACALPGGLCAPQVQVDCVRAELERIGTILSGLAGQPLDTETL
                     ARGIRQANQVRRLLAALRRVVYTSPGAPLPALELLVAEMLALHFCSDREETIGVLTDL
                     LAEAEARLRQKAFVVADPAVRVFWVNPVADLRAMNLLEDWGGRVCGSDYMFTHALDPI
                     PEDLPPLEALARTALADPMVGSTQERARRIVADCRAGRAEAVVVSRIPGASHCAREGA
                     IIREVVRADLGIPIIELEVPPICDAMLPTLASRLQALLETAKSRR"
BASE COUNT          498 a          806 c          864 g          394 t
ORIGIN      
        1 ctcgcaggtt acgagcaact tcggctactg caaagcagaa gactccaatt ggaaatctcc
       61 gcccatcgtc tctgcggttc attcctacgt gccccgactg aatagacacg gctcagagca
      121 gggggccagg gcgggactgg cgactggcca ggatggcggc gcccagtgcg ccggtatatt
      181 gcgggaggga cgagacacat acggcgcagg acagggccgt ttccagggct cggaccatgc
      241 cggattgcag cgcgaccccg ccggtaaagc agacgggcgg cgtgaccgtt cttccggcca
      301 gtgtcacaac ccgctgggcg atggcattct gcacgccagc ggcaatgtcg gctggcgcga
      361 cgccttcggc cagcagcccg atgatctcgg tttcggcaaa gaccacgcac atgctgctga
      421 tgagtgcagc ctgatggctg tgccggctga ggtctcccag cgtggagaga tcgacgccca
      481 gccgggtggc gacgacttca aggaagcggc cgctgccggc ggcacagcgg tcgttcatgg
      541 caaagtcccg cacgcggccc ccgtcttcga gtgtgatgaa cttgctgtct tggcctccaa
      601 tctcgacaat ggtgtgaact tccggcgcca ggtgatgaac gccctgcgca tggcaggtga
      661 tctccgtaat ggttgtgtcc gcaaaccgga tggcgttgcg gccatagccg gtggcaacga
      721 tggccttgac tgcagcccgg tccacttgcg cgctctggca ggcgcgttcg agcagttcgg
      781 aagccaggcg ctgctgttcc acgccctgat cggcaaggct ggagccaacg atgcgcgact
      841 gttcggaatc gaacagcacc actttggtcg cccgcgaccc tgcatctatg ccggcacaga
      901 tcatagggga aacccgaaat ccgaaggccg aaggccgaag gaaacccgaa ggccgaaatc
      961 caaaggccga agggtaatgg gcagagtcat agcgcgctgg ctaaggttgg cacccaagag
     1021 cccccgcgag agccggggca cccggtgttt caattcacat ctttcagtca tttcagcggc
     1081 gcgacttcgc ggtttccagc agtgcctgca ggcgcgaggc cagcgtcggg agcatggcat
     1141 cacaaatggg gggcacttca agctcgatta tgggaatccc caggtcggcg cgcactacct
     1201 cgcgaatgat agccccctcg cgcgcgcagt ggctggcgcc gggaatgcgg gaaaccacca
     1261 ccgcttccgc gcgaccggcc cggcagtcgg ccacgatgcg gcgggcccgc tcttgggtgg
     1321 agccgaccat gggatcggcc agggcggtcc gggcgagcgc ttcgagcggg ggcaggtcct
     1381 cgggaatcgg atccaaggcg tgagtaaaca tatagtccga cccgcagacg cgcccgcccc
     1441 agtcttccag caagttcatg gcgcggaggt cggccacggg gttgacccag aagacgcgca
     1501 cggctgggtc agccaccaca aacgccttct gccggagacg ggcttcggcc tcggccagca
     1561 aatcggtcag cacgccaata gtctcttcac gatcggaaca gaagtggagg gcgagcatct
     1621 cggcgacgag caattccaag gctggtagcg gggcgcccgg cgaggtgtaa accacccggc
     1681 gcaacgccgc gagcaagcgc cggacctggt tcgcttggcg gataccgcgg gcgagggttt
     1741 cggtatcaag cggctgtccc gcaagcccgc tcagtattgt gccgatacgt tccagttcgg
     1801 cccgcacgca atcaacttga acctgtggcg cgcacaagcc gccgggcaga gcgcaggcgg
     1861 cttcgcccgg gtccggtgcc cggcggcggg gcatctccca ccagacaatg gggaaactca
     1921 tcttctccag gcgttgcgcg atagccgaga agtcatcaca gacggcgccg gtgctgcaga
     1981 tcagcgcgtc cggccgcggg aagtgctcgc cgttctgaaa cgcgggcagc atggcgcgga
     2041 cagggcaaaa ggacgcgtcg atgcccagtt gctccgcctg ctggagcaag ccgtcgctgc
     2101 attccatcag gcagggggtc caccacgcgc cgtccgggta gaacgcccgt aggcccggca
     2161 gcgaatatgc cattactggc accgtgccga ggtctttcat ggcgccgatg atcttctcgc
     2221 cctgcgcgcg ggccgcatgg aggcgctggt tctcggtcag cagaaagttc caaaggcgca
     2281 gggcggcggc ggaattgtca aagcggaggt ggcgcaagcg caaatcacct tcaagcacga
     2341 catggcggcg cagcggcccg ccatagccag gctcgcgcag cccggcccgg gtgagttgct
     2401 cgtaacgggc atcccagtcg tcgagggtga gcttgcgcgg cggagaggtc atggggaaat
     2461 ccgaaacccg aaggccgaag gtcgaaggaa acccgaaggc cgaaatccga atggcgctgg
     2521 gcggaccggc acgaagctca caggccctgc cccggggaaa cc
//
LOCUS       DATCGS010000262        16778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_568048, whole genome shotgun sequence.
ACCESSION   DATCGS010000262 DATCGS010000000
VERSION     DATCGS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16778
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568048"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIL39_05320"
     CDS             <1..198
                     /locus_tag="VIL39_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480880.1"
                     /translation="RSSRLPAEMITLNDRTVGLAPQGEVGAESRQRLWQCSRALRQNT
                     GAGSYNTSRLRLQTTNSPPRK"
     gene            613..2235
                     /locus_tag="VIL39_05325"
     CDS             613..2235
                     /locus_tag="VIL39_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480881.1"
                     /translation="MSQIIVPLENLFAPGIAPDPNNADPMMIALQVARRYPFLPQPVF
                     IEVEGDRIILNYPAAFEDAKKEAERLAQRAARHAGNGDCHRAIAIWEQVLQLVPDDLV
                     ARRDIGEACSELGDFIKAKHYLSEALLVDFEDIGSLVALANLAVRQEDYGAAEVYAQK
                     AVAAGPQNAWALNCLGAVQFHTGRHEEALSFLRAAIAFDPEFAPPYGVIAYLSHQQGR
                     HAEAEATLREMFARAKKQDIRSEPIFTQARQLYRKVQDSLAEMQREQARRTAEEFRVA
                     VEKHTGYPIRVVQEGSGDGPVAQVQLAWQHHRDHHLIRCQPSCPSKLMPHLLAHEIMH
                     IRLGYDAHQAERCRYFSLSSANRQAALQAFENRLERLAREGYPVVWLADCTGRLIANL
                     FGALYNYPLDLVVEQKLREKMPTLAPTQFLALTTPLLKEEEQDDYELISRLMPRRFLR
                     ALVALRGVQLLFVDSLCCGATDHAARFRHREGFNLARRLWQHWQTKSQALQPGDEYDL
                     VDEFADIVGLRGNYTWLPDPHSNSSPPTPAAA"
     gene            2246..2797
                     /locus_tag="VIL39_05330"
     CDS             2246..2797
                     /locus_tag="VIL39_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480882.1"
                     /translation="MQDSPYLMPAQTNNRANTERRFTHRRMSSCRLEGSAAHKSPPKT
                     KAMKRHVHNQPTRHINISSLARRFGIPIRTAITPELVACYGQSIDSVAAHPAWLWDLL
                     WTARHALAGQLPCRRESTESAELYHIGCSTFFSGRMEPEHIRCCVALSGEDDGDVRLT
                     FRLSAPEPKLYAKENSSDKSSTL"
     gene            2794..3486
                     /locus_tag="VIL39_05335"
     CDS             2794..3486
                     /locus_tag="VIL39_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480883.1"
                     /translation="MKIKILGLALFEIQRDLPTTFSGRPKAPPAKPVKSRRPTVMVPT
                     TLLPLADLHKPADLLPRTARGTLDADAALRLFGLKDVAVLWQQKYPSGSAGAALKRVV
                     GAIGRKPARRAIRVRLGLEGLSPGASPDTERPPAPLRPPKPETNLPQPATKVSAAQDS
                     SALPLPRDASGRIDIAASLELLGLPDPKTLWNGKVQPGSPAHELKKEIARVLARGETV
                     TGITRQDIGLPD"
     gene            3495..4370
                     /locus_tag="VIL39_05340"
     CDS             3495..4370
                     /locus_tag="VIL39_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480884.1"
                     /translation="MPTPIFQPGDEILFRENGIEIRGVVSLVHGSQVTFLAEDGRKLT
                     RNSRLVTHLCPMAAQRRVLPLPPIHERLTLLSQAVEMVASKLSPSLILVGPTGGKTYE
                     VTRTLRAMGLERDHDFFLIKGYASARGLYETLYRHNGCILVFDDCDNALTDAVAVELL
                     KGALDSYDARTIAWLTAAKGGGQIPPRFDFTGSVIFISNRALADIDEPVHSRSLVIDL
                     QMSRAEILERMETLLPRVEINATAEQRRLAMNFIRQWAPNIQQLSLRTLIYLLRIIQA
                     NPRNWERLASYAVTR"
     gene            4415..5005
                     /locus_tag="VIL39_05345"
     CDS             4415..5005
                     /locus_tag="VIL39_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5480885.1"
                     /translation="MKIKIPGPTVVDIGKDHPAPPAPPAESRPPTAIAPTVPGPPADL
                     DQPARPRRPHLLVVEDDEDVADCLRLGLDRKGFAVDVALTAAAAQECLQRRLPELILL
                     DVDLPDLSGLELCRQLKAGPQMARIPIVFCSGNDDARTRALLLGAADCFEKPPDLTQW
                     PDVCTRWWATSLRPKQLKSKSHELIHSRQLRSRAYP"
     gene            5049..5441
                     /locus_tag="VIL39_05350"
     CDS             5049..5441
                     /locus_tag="VIL39_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480886.1"
                     /translation="MKPDYLQNGCTRAEALLDGQLHDLTRIKAAINCRFIRPTAMTQA
                     LWHALAGDTTFALDHPHLLQLCRLVVSAIATGQAERRKQGMFSETIWLRATLGGRPLH
                     LKVVCHPGDAGEPVLTLLEGSEATQFEI"
     gene            5625..5834
                     /locus_tag="VIL39_05355"
     CDS             5625..5834
                     /locus_tag="VIL39_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480887.1"
                     /translation="MSENIRSEREKREQEALDAFLGRSVKTKAPAEMVWLFPFLDQPG
                     LQCPSLRPSVADGLLLALEAHRHIG"
     gene            5837..6634
                     /locus_tag="VIL39_05360"
     CDS             5837..6634
                     /locus_tag="VIL39_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480888.1"
                     /translation="MKPTRSIISLDSGLLIRALQGDSAPKATRGSRLDRQWRPSAIRA
                     AMSKFFSGHEPHWALHAAQLLELATVVANLAQSGKIRYRDNGRVKERALTAEESANVR
                     EFRHYLEALRNQLQSNLVVPKICYTSMEGEGLGLYMIASTLHDLAVEFIKASCGKLTN
                     PIVDTVICLNAYHQACLHRYGYAHQILTMDGNLGVIQKLIRDHVFDCVRRGSARDCDA
                     LPEPLSSFRALFCWREGGRKTRIMLPNIKEAFDIQDFLCRPPKGRSC"
     gene            6913..7443
                     /locus_tag="VIL39_05365"
     CDS             6913..7443
                     /locus_tag="VIL39_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480889.1"
                     /translation="MKTKQLREGDLLFYSFSRPTVEDGDFRHFLDTFGLEHLPRDQAL
                     RSLCNSVVFILDGYDHDTRELYEIPHVRQFCTSFSQAWPFWLFACNLEFPSLQAFTFS
                     CLPCLRVIKRDGAGLLRVQYPPQELGKFLEPHFAGMNLLFERAGMTEAENRQRTQQIL
                     EFYQRDWTSFAQMTSP"
     gene            7477..8157
                     /locus_tag="VIL39_05370"
     CDS             7477..8157
                     /locus_tag="VIL39_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480890.1"
                     /translation="MINKSRTPTVPSQEAGGPSATELLEAITPEQEQQLQAFARRRLR
                     RAANTPWLQRHLAPNSAEDLVQEAKAKVLLGEQFPRVGRRLKPRNRVSVEACLACLRG
                     IMESDLSNLLKVARRRGEHLSVGDSRTEPGTVDPPDPLDPAGNLVRRDLRCVGFARLR
                     ERAAAEPDLLPVILQWEEGFLTDDRIAAEELDRKPAHRVRVMFREVLQELAAELSVAP
                     VTGKEMLL"
     gene            8154..8549
                     /locus_tag="VIL39_05375"
     CDS             8154..8549
                     /locus_tag="VIL39_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480891.1"
                     /translation="MKRRIPPAGVTESVCPGSRAAELRAGVLHDISATARACAQLDVP
                     TAITHRLWEAVAPGESFRANDERVKEVCYALAFARVGLIDGELFDQAGGQAMRFEFLH
                     REKSLSLTVVVHPAENLTLVCTIMHSHEA"
     gene            8703..10238
                     /locus_tag="VIL39_05380"
     CDS             8703..10238
                     /locus_tag="VIL39_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5480892.1"
                     /translation="MSSNYDPMDSFYTRAKGLLDAQPRLGKKKLARLLQAHQGHARRL
                     LERWRGETQGPGNNPEYLSVEQVKRAHPRWGARRIAHALGLTLDRAKLHLARWTGVQS
                     RSQTAGTASNAATLPPPSQTKAAPAPPLEPPASMPEAPANGSELQDTVRPESRDLCYR
                     GTRIHTLEDLLVFAEVNTSLWEVERHVINKWEVGARNPATGEILVEQLFQVKAWLKRR
                     VAERRLEDLLNSMLAEFRKAAPEWPALSRPSAGAGTLEVSLMDLHLGKLAWGPETGGR
                     SYDASIMENMFWAALEDLLDKASGCKPGQILFVAGNDFFNTDSLGRTTTAGTPQDEMV
                     RWQDSFTRGRNLLVKAIERMRTFAPIKVLMVPGNHDTQRLYYLGEVLHAWFHRTADMQ
                     ADYSPATRKYFAHGANLIGFTHGNNERHPNLPLLMATEQPEKWAKSRHREFHLGHFHT
                     KKHKMFVPAEDLSGVLVRILPSLCPPDAWHASMKYSSKLAAEAYYWDPEDGCVATFTH
                     SPA"
     gene            10235..12211
                     /locus_tag="VIL39_05385"
     CDS             10235..12211
                     /locus_tag="VIL39_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480893.1"
                     /translation="MNIPINFPDDEPPLVRLKCYEPPGLTLELDRDTLLRHLLFIGGT
                     GAGKTSVVNGVLAQLLAAQVNGRPIGLLILDGKQDESVARVQKLAAEAGRSVLVLTPD
                     GELRYDLFASLRTLDDVEPTAQRLLLGAGRLGGDNLYWSDMRLTLVDAALSLLVAFGV
                     PVAFETAVEFMRRWFFGGTTDAELVSKVIQHTTRVLAEGEARLPAAIRRKLQQALDTA
                     ALWRTLDPRTRSNVQSTLLAAMRPLLSRRASLLFEADGHVCFRAGSVLEQGGVVVVSA
                     NAVNEPDLARLLFRLVKSDFYRAVQARCSGSEPLAFLIMDEYPLAASQSDLPNLQTLR
                     SKNAGVIACTQGFISLDEALGVRIRQALIAGFQNIIAMRSHEIEIDALMFGMMGEREL
                     EEPSRGGHRYEDLIDWSPGPPRRRPVPVCPLGALARLKPFEAFVSLANGTHPPQSVWL
                     EPLFVAVEPASPSIVLAEPADPLAEVVARIRGGVVGPASTSSIFELMGRVGGQMLMPH
                     PLWQAAVELCVPGMSQELLVEQLTSFFRVRSGLCPRGLESLPACWLRALPRLLEKRAK
                     KHWQGRIPFGLSSVAEQGGALVMEFAQEHECPRREGEVNSWDVLRVHMNCHLYPSLWQ
                     PLTRRHWRQLHATRPELRAHLRVHAIVVGSSWLQ"
     gene            12886..13173
                     /locus_tag="VIL39_05390"
     CDS             12886..13173
                     /locus_tag="VIL39_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5480894.1"
                     /translation="MKQPLSNKVAIVTGASRGIGRAIALKLAEDGAAVMVAARNAELL
                     KEAQAEIESNGGKAAWLSLDLRKPEAPALLVQNTIEVPGRVCPRDRVEQER"
     gene            13124..13393
                     /locus_tag="VIL39_05395"
                     /pseudo
     CDS             13124..13393
                     /locus_tag="VIL39_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
     gene            13431..14015
                     /locus_tag="VIL39_05400"
     CDS             13431..14015
                     /locus_tag="VIL39_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480895.1"
                     /translation="MQIPELHRYSVGELYSEKRRRWPEGAQFLYRRGQFEFLLFFNRP
                     RGQEVLDVRKGEARFALLPYRDTLFFLYRFGTNPRWSDATFSWWLHEAEERQVPIPPQ
                     ELAEESRIAIPIMLVGADDGLIKAMRLVTFSPSFTAELFRAVRKQASQPWCGDEAYAK
                     TLRRPTACGPRPTGWPRRQQPSAVVGIDLITLTQ"
     gene            14012..14209
                     /locus_tag="VIL39_05405"
     CDS             14012..14209
                     /locus_tag="VIL39_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480896.1"
                     /translation="MNKTNKTNTDNTFQQGLEGTGAGEWAKVSYNMQLVVLTTAATAT
                     PEVTLGILTGYPAWRIGQTCG"
     gene            14200..14943
                     /locus_tag="VIL39_05410"
     CDS             14200..14943
                     /locus_tag="VIL39_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480897.1"
                     /translation="MRLIPERVAEAADKFCGTSVMYPSTHDITPEILPQALQTLRGLL
                     GGGNSVLFVSKPHLEVIEAICREMPAHKTQLQFRFTIGSGNPDTCALWEPGAPGPNER
                     IAALRHAFELGYQTSVSMEPMLEDNAAMCALVDRVTPFVTGTVWLGKLNGGVPLYIQA
                     KPGVKEALRAVRAGQSDELILALHDRLAQNPKVRWKDSIKEVLRKHGRLPKLPNASAK
                     VVDPKRRQAALKAHETMRRRRAALQGTSG"
     gene            complement(15000..15269)
                     /locus_tag="VIL39_05415"
     CDS             complement(15000..15269)
                     /locus_tag="VIL39_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480898.1"
                     /translation="MSAPTIKAIAAMAALDALINEDKTLICLTGPAATGKTLLGIASA
                     LHSARTRAPEPAQEQADPEADVTSDEQHLTRKQRKMMENRRGRRM"
     gene            complement(15511..16323)
                     /locus_tag="VIL39_05420"
     CDS             complement(15511..16323)
                     /locus_tag="VIL39_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480899.1"
                     /translation="MKRKYSPRELKPVLQALAQTNGVIIGGQAINLWAEHYQKDSSPW
                     KELRPYTSFDLDVLGNRTDVLKCSQALNAEPFFPTPAENTVNSGKIVTQIQGSEFEID
                     FLHSPNGLSPAEVVELARPISFEQMSLKVLHPLHCLESKTVNLMTLPQEAGERQDLKH
                     LHLSIAILRQHLTDLTLKGGAEQLLLRWAHRIRTNSNHELGIQAAIKHGINFQDAIPA
                     DLWRDRPGPLADFVKREWSDWKDENDEKIADLRDLESWLKSLNASPKGPDLE"
BASE COUNT         3261 a         5297 c         5045 g         3175 t
ORIGIN      
        1 cgaagctcac gcctacccgc cgagatgatt accttgaatg accgcaccgt gggcctcgcc
       61 ccgcaaggtg aagtcggcgc cgaatctcgc cagcgcctct ggcaatgctc ccgtgccctg
      121 cgccagaaca cgggggctgg atcatacaac acttcgagat tgcgcctgca aacgacgaac
      181 tcgccaccgc gcaaatagta ggtgcccggc gtgccgccta atttctgtct gataaggcac
      241 tcgcgacccg cgaacccatc cgccgtttcg ggaaatgaac gcaggcgcgg ttcgttggtc
      301 ttcggcatcc ttccaggaca accgacgctc gccacggaga ctaggtttgg cgaaagcagc
      361 tcttcgtatt tgcgcatcct ggtgagttcc gtcacttcgt caaccactgc ttaactcgcc
      421 gctggcaacg ctggaactag cagcgagcaa ggttgacgag accgagagct gttcccccgc
      481 atcaaggccc ggcattcggc tacgccgatc agactcccac cgaagacatt tcaccgagca
      541 gtcaaatccg ttcccggcct cggatcggcg cccaacagaa aagcagacaa acctgatgct
      601 gttatgaatc aaatgagcca aatcatagtt cccctggaga atctgtttgc gcccggaatt
      661 gctccggacc cgaacaacgc agaccctatg atgatcgccc tgcaggtcgc gcgtcgatat
      721 ccatttctgc ctcaaccggt cttcatcgaa gtcgaggggg accgaatcat cctcaattat
      781 cctgcggcat tcgaggacgc caagaaggaa gcggagcgct tggctcagcg tgccgccagg
      841 cacgcgggta atggcgattg ccaccgggcc attgccatct gggaacaggt gctacaactc
      901 gttccggacg accttgtggc acgccgggac attggcgaag cttgcagcga actcggcgat
      961 ttcattaaag ccaagcacta cctctcggaa gccctcctcg tcgactttga agacatcggg
     1021 agcctggttg ctttggcaaa cctcgcggtc aggcaggagg attacggggc ggctgaggtc
     1081 tacgcccaga aggcggtggc cgctggccct cagaacgcct gggcgttgaa ctgcctcggc
     1141 gcggtccagt ttcacactgg acgtcacgaa gaggccctca gcttcctccg ggcggccatc
     1201 gcatttgatc ctgagttcgc tcctccttac ggcgtcattg cttacctgtc tcaccagcaa
     1261 gggcggcatg cggaggcgga ggccaccctg cgcgagatgt ttgccagggc caagaagcag
     1321 gacatccggt ctgagcccat cttcacgcag gccaggcagc tttaccggaa ggtccaggat
     1381 tcactggcgg aaatgcagcg cgagcaagcc cgccggacgg cggaggaatt tcgcgtcgcg
     1441 gtggagaagc acactggcta ccccatccga gtggttcagg agggttctgg cgacgggccg
     1501 gttgcccagg tacaattggc ctggcagcac catcgggatc atcatctcat ccgctgccag
     1561 ccgtcctgtc caagcaagct catgcctcat ctgttggccc acgagatcat gcatatccgg
     1621 ctgggctacg atgcccacca agcggagcgc tgccgttact tctccttaag ctctgccaac
     1681 cgccaggcgg cgctacaggc tttcgaaaac cggctcgaac ggttggctcg cgaggggtac
     1741 ccggtggttt ggcttgcgga ctgcaccggg cggttgatcg caaacctgtt tggggcctta
     1801 tacaactatc cgctggacct ggtggtggag cagaagttgc gcgagaaaat gccgacgctt
     1861 gcgccaaccc aattcctggc gctgaccact ccgctgctaa aggaggaaga gcaagatgat
     1921 tatgagctga tttcgcgcct gatgccgcgc aggtttttgc gcgccctggt ggcactgcgc
     1981 ggtgtgcagt tgttgtttgt ggattccttg tgttgcgggg ccaccgatca cgcggccaga
     2041 ttccgccacc gggagggttt caatttggcc cggagactct ggcaacactg gcaaaccaaa
     2101 tcccaggcgt tgcagccggg cgatgaatac gacctggtgg acgagttcgc ggacattgtt
     2161 gggctgcgcg gcaattacac gtggcttccg gacccgcatt ccaattccag cccgccgacc
     2221 ccggccgcag cttaagcgag tgacgatgca agattctccc tacctcatgc ctgcgcagac
     2281 aaacaaccgc gccaataccg agcgtcgttt tacgcaccga aggatgtcct cctgccgcct
     2341 cgaggggagt gctgcgcaca aatcgccgcc aaaaacaaaa gcaatgaaac gtcatgttca
     2401 caaccaacca acacgtcaca tcaacatcag ttccctggcg cggcgcttcg gcattcccat
     2461 tcgcaccgcg atcactcccg aattggtggc ctgctacgga cagtcgatcg acagcgtggc
     2521 cgcccacccg gcttggctct gggatttgct ctggaccgcg cgccatgcac tggccggcca
     2581 gctaccctgc cggcgcgaat cgaccgagtc cgccgagttg tatcatattg ggtgttccac
     2641 cttcttctcc ggccggatgg agccagagca catccgctgc tgtgttgccc tgagcggcga
     2701 ggatgacggt gacgtccgcc tgacctttcg cctctcggca cctgaaccca aactctacgc
     2761 gaaggaaaac tcatcggaca aaagctcaac gttatgaaaa tcaaaatcct cggactggcc
     2821 ctgtttgaaa ttcaaagaga cttgccgaca acgttctcgg gacgacctaa ggcgccgcca
     2881 gcaaaacccg taaagtcacg acgaccaacg gtcatggtcc cgaccacgct ccttcccttg
     2941 gccgatctcc acaaaccggc cgaccttctg ccgcgcaccg cccgagggac cctcgatgcg
     3001 gacgcggccc tccggctgtt cggactcaag gatgtggctg tcctctggca acagaaatac
     3061 ccttcgggga gcgccggcgc ggcgctcaag cgagtggtcg gcgccatagg ccgcaaaccc
     3121 gctcggcgtg cgattcgggt gcgcttgggt ctggagggcc tttcgcccgg cgcgagccct
     3181 gacactgagc gtccgcctgc acccttgcgc ccacccaagc cggagaccaa cctaccgcaa
     3241 cccgcgacca aggtttcggc ggcgcaagac agcagcgcat tgcccctccc gcgagatgcc
     3301 agcgggcgca tcgacatcgc cgcttccctc gagttgttgg gccttcccga cccgaaaacg
     3361 ctatggaatg gcaaggtcca gcccggctca ccagcgcatg agctgaagaa ggagatcgct
     3421 cgcgtcctgg cccgaggcga aacggtaacc ggcatcacgc gccaggacat tgggttgccc
     3481 gattgaaccg ctttatgcct acacccattt ttcagcccgg cgacgagatt ctgttccggg
     3541 aaaacggaat cgaaatccgg ggagtcgtca gcctcgtgca cgggtcccaa gtcacctttt
     3601 tggccgagga cggtcgcaag ctgacgcgga actcccgcct ggtcactcat ctgtgcccga
     3661 tggccgcgca gcgcagagtg ctgccgcttc cacccatcca cgagcgtctg acgctcctgt
     3721 cccaggcggt ggagatggtc gccagcaaac tctcccccag cctcattctg gtgggtccga
     3781 ccggggggaa aacctacgag gtcacgcgca cgctccgggc catgggcctc gaacgcgatc
     3841 acgacttttt cctcatcaaa gggtacgcct ctgcccgcgg cttgtatgaa acgctttacc
     3901 gccacaacgg ttgcatcctg gtctttgatg actgtgataa cgcccttacc gatgcggtgg
     3961 cggtggagct gcttaaaggc gcgctggact cttacgacgc gcgcaccatc gcctggctca
     4021 ccgccgccaa gggaggcggg cagattccgc cacgcttcga tttcacgggc agcgtgatct
     4081 tcatttcaaa ccgcgcgctg gcggacattg atgaacccgt ccacagccgc agcctcgtca
     4141 tcgacctgca gatgagccgc gcggaaatcc tggaacgcat ggaaactctg cttccccggg
     4201 ttgaaatcaa cgccaccgcc gaacagcgcc ggctggcgat gaacttcatc cgtcaatggg
     4261 cgccgaacat ccagcaactg agcctgcgca ccctgatcta tttattgcgc atcatccagg
     4321 caaacccgcg caattgggag cggctggcca gctatgccgt cacgcgatga gcccggatca
     4381 ggaggaacag ctcaaccaac aaaaaatcca cgttatgaaa atcaaaattc ccggcccaac
     4441 cgttgttgac atcggaaaag atcacccggc cccgcccgcg ccacctgcgg agtcacgccc
     4501 tcccactgcc atcgcgccga cggtgcccgg tcctccggcc gatctcgacc aaccggccag
     4561 gccccggcgg cctcatctcc tggtggtgga ggacgacgag gacgtcgccg attgtctgcg
     4621 cctgggtttg gaccggaagg gttttgcggt cgacgtcgcg ctgaccgcag ccgccgccca
     4681 ggagtgtttg cagaggcggt tgccggagtt gatcctcctc gatgtggatc tgcctgactt
     4741 gagcgggctc gaactctgcc gccaactcaa ggccgggccg caaatggcgc gcattccgat
     4801 tgtgttctgc agcggcaacg atgacgcgcg gacgcgggcg ctcctgctgg gggctgcgga
     4861 ctgtttcgaa aagccccccg acctcacaca gtggcccgac gtctgcacgc gctggtgggc
     4921 caccagcctg cggccaaaac aacttaaatc aaaaagtcat gaactcatcc actccagaca
     4981 actccgatcc cgggcgtatc cgtgaatccg aatcacaccg aactgacctt aacgaaaacc
     5041 acacacctat gaaaccagac taccttcaaa acggctgcac ccgcgcggaa gccctgctcg
     5101 acggacaact gcacgacctc acgcggatca aagcggccat caattgccgg ttcatccgac
     5161 ccaccgccat gacgcaagca ttgtggcacg ccctggctgg cgacacgacc ttcgcgctgg
     5221 atcatccgca tttgttgcag ctttgccggc tcgtggtgtc cgccattgcc acgggccaag
     5281 ccgagcgccg gaagcaaggc atgttcagtg aaaccatctg gctgcgcgca acgctgggcg
     5341 gccggccgtt gcacctcaag gtggtttgcc atcccggcga cgcaggggaa ccggtgctca
     5401 cgttgttgga gggttcggag gcaacccaat tcgagatctg aggccgcatc aacggtgtca
     5461 ttcacggcgc aagcataccg gttgctaagc gtgttgtcgc gatcgccccc acacgcccag
     5521 cgttccaacc gcatgctctt aagtcagcgg gcctggggct gatgccaatg gaatagaccg
     5581 cctcggctaa aacgaaaaat gtgttaggcc gcctttgcta aaagatgagt gaaaacatta
     5641 gaagcgagcg ggagaaaaga gagcaggaag ccctggacgc ctttctggga cgcagcgtga
     5701 agaccaaggc ccctgccgag atggtgtggt tattcccttt cctcgaccag cccggcctcc
     5761 aatgcccgtc cctccggccc tccgttgcgg atggcctcct gctggcgctg gaggctcacc
     5821 gtcacatagg ctaaacgtga agcccaccag gagcatcatt tccttggaca gcggcctgct
     5881 catccgcgcc ctccaggggg actcggcccc caaggccacc aggggcagca gactggaccg
     5941 ccagtggcgc ccgtccgcaa tccgcgccgc catgtccaaa ttcttcagcg gccacgaacc
     6001 acactgggca ctgcacgcgg cgcaactgct cgagttggcc accgtcgtcg caaacctcgc
     6061 gcagtccggc aagatcaggt atcgggataa cgggcgagtg aaagagcgcg ccctgaccgc
     6121 ggaggagagc gccaacgtgc gcgaatttcg ccactacctc gaggctctcc ggaatcagct
     6181 ccagagcaat ctcgtggtcc ctaagatctg ctacaccagc atggaagggg aagggctggg
     6241 actctatatg attgcatcga cgctgcacga ccttgccgtg gagttcatca aagcatcttg
     6301 cggcaagctc accaacccca tcgtcgacac cgtcatctgc ctcaacgcct atcatcaagc
     6361 atgccttcac cgctacggat acgcgcatca gatactcacg atggatggga acctcggcgt
     6421 catccaaaag ctgattcgcg accacgtctt tgactgcgtc cgccggggat ccgcccgtga
     6481 ctgcgacgcg ctccccgagc cgctttccag cttccgcgca ttattttgct ggagggaggg
     6541 cggccgcaag accaggatta tgctccccaa catcaaggag gcattcgaca tccaggactt
     6601 tctctgccgc ccgccgaagg gcaggtcgtg ctgaattccg attccaaagc ccctcggtcc
     6661 tcgatctttc cgacgcatgg ttgagacgac gcccgacaag gggcgtaatg caatgtgaaa
     6721 ggagagctcg gcgcgcggcc cctgcaggag gtggccgtct aatccgttgc caggtccaaa
     6781 caaaaggcaa agccccttcg tgttcgcgac ccgcctgacg ttcgaggctt ggctcttttc
     6841 accgggccca ggctttttgt tttctcggtt cgcgcctacg gccaaaacag gtaggcagac
     6901 aaaaaactca ccatgaaaac caaacaactt cgcgaaggag accttctctt ctattcattc
     6961 agccggccga ccgtcgagga cggcgacttc cggcacttcc tcgacacttt cggtctggaa
     7021 cacttgccgc gcgaccaggc cctccgttcc ctgtgcaaca gcgtcgtgtt catcctcgat
     7081 ggctacgatc acgatacccg cgaactttac gaaatccccc acgtgcggca gttctgcacg
     7141 tcgttcagcc aagcgtggcc gttctggcta ttcgcttgca acctggagtt tcccagtctt
     7201 caagccttca ccttctcctg tctcccctgc ttgcgggtga tcaagcgcga cggggcaggc
     7261 ctgttgcggg tgcaatatcc cccacaggaa ctgggaaagt ttttggaacc gcacttcgcg
     7321 ggcatgaacc tgttgttcga gcgcgccggg atgaccgaag ccgagaaccg ccaacggacc
     7381 cagcagatcc tggaattcta ccagcgggac tggacttctt ttgcccagat gacctccccc
     7441 tgatccggag aaattaccgc caaacacttt gccgaaatga tcaataaatc gagaacacca
     7501 acggtgcctt cgcaggaagc cggcggccca agcgccaccg aactgctgga ggcaataacc
     7561 ccagaacaag aacaacagct tcaagctttc gcgcgtcggc gcctgcgccg cgcggccaac
     7621 accccgtggc tgcagcggca tctcgctccc aacagcgccg aagacctggt gcaggaggcc
     7681 aaggccaagg tgctcctggg cgagcagttt ccgcgagtcg gacgccgctt gaagcccagg
     7741 aaccgcgtca gcgttgaggc ctgcctcgct tgcctgcgcg gcatcatgga gagcgacctg
     7801 tccaatctgc tcaaggtcgc ccgccgccgc ggcgagcatc tgtcggtcgg cgacagccgc
     7861 accgaaccgg gtaccgtgga tccgccggac ccgctggacc ccgccggcaa cctggtccgg
     7921 cgggatctgc gctgcgttgg ctttgcccgg ctgcgcgaac gcgccgccgc cgagcctgat
     7981 ctgctgcccg tgatactgca gtgggaagag ggtttcctga ccgacgaccg cattgcggcc
     8041 gaagagctgg accggaagcc cgcgcaccgc gtgcgcgtga tgttccgcga ggttttgcag
     8101 gagctggccg ccgagctgag cgtcgccccg gtaaccggaa aggagatgct cctgtgaaac
     8161 gacgcatccc cccagccggc gtcacggaat cggtctgccc cggctcgcgc gccgcggaac
     8221 tgcgcgccgg ggtcttgcat gacatcagcg ccacggcccg cgcctgcgcc cagctcgacg
     8281 tgcccaccgc catcacccac cggctctggg aggccgtcgc cccgggcgaa tctttccgag
     8341 ccaatgacga gcgggtcaag gaggtgtgct acgcgctggc cttcgcgcgg gtgggcctga
     8401 ttgacggcga gctgtttgac caggccggcg gccaagcgat gcgcttcgag ttcctgcacc
     8461 gcgaaaagag cctcagcctg accgtggtcg tgcatcccgc ggagaacctg acgctggtct
     8521 gcaccattat gcacagccat gaagcctaag ctgcgcaacc ctgacttcat ctcgcgggcg
     8581 cgtgggccac caacccgccg tgaaatcatg acgggtccaa gctcattccc ggaaaagcgc
     8641 agtttttcgc atttttccgg gaacactttt tgttctttgg agcatataca gggtgagagc
     8701 ttatgtcgtc aaactacgat ccgatggatt cattctacac gcgggccaag ggcctgctgg
     8761 acgcccagcc gagactgggc aagaagaagc tggcacggct gttgcaggcc caccagggtc
     8821 acgcgcggcg cctgttggag cgctggcggg gggaaaccca gggccccggc aacaatccgg
     8881 aatacctcag cgtggagcag gtcaaacgcg cccatccgcg ctggggcgcc cgccgcattg
     8941 cccatgccct gggtctgacg ctggatcgcg ccaaactgca cctggcccgc tggacgggag
     9001 ttcaatcgcg ttcgcagact gccgggacag cctccaacgc cgccacgctt ccaccgccgt
     9061 cccagaccaa agctgcgccg gcgccgccgc tcgaaccccc cgcttcgatg cccgaggcgc
     9121 ccgccaatgg cagcgaactg caggacacgg ttcgtccgga atcgcgcgac ctctgttacc
     9181 ggggcacccg cattcacacc ctggaagact tgctggtgtt tgccgaggtc aacacgagcc
     9241 tctgggaagt cgagcgccac gtgatcaaca aatgggaggt cggcgcccgc aatccggcca
     9301 ccggcgagat cctggtggag cagctcttcc aagtcaaagc ctggctgaaa cgccgggttg
     9361 ccgagcgccg cctggaagac ctgctcaact ccatgctcgc tgagtttcgc aaagcggctc
     9421 cggagtggcc ggcgttgtcg cgcccatccg ccggagctgg cacgttggag gtatcgctca
     9481 tggacctcca cttgggcaag ctcgcctggg ggccggagac gggcggccgc agctacgacg
     9541 cgagcatcat ggagaacatg ttctgggccg cactggaaga cctgctggac aaggcttccg
     9601 gttgcaaacc cggtcagatc ctgttcgtcg cgggcaatga ttttttcaac acggactcgc
     9661 tcggccgtac tacgacggct ggcacgcccc aggatgagat ggtccgctgg caggactcgt
     9721 ttacccgcgg acgcaacttg ctggtcaaag ccattgagcg gatgcgcacg ttcgcgccaa
     9781 tcaaagtgct catggtgccc ggcaaccacg atacccagcg cctgtattac ctgggggagg
     9841 tgctgcacgc ctggttccac cgcacggcgg atatgcaggc ggattactcc ccggcgaccc
     9901 ggaagtactt tgcccacggg gcgaacctga tcggctttac gcatggcaac aacgagcggc
     9961 atccaaacct gccgctcctg atggcgacgg agcagccgga gaagtgggcc aaatcacgcc
    10021 accgggaatt ccatctgggc cacttccata ccaaaaagca caagatgttc gtgcctgcgg
    10081 aagatctgag cggcgtgctg gtgcgcatcc tgccctccct ctgcccgccc gatgcctggc
    10141 acgcctccat gaagtattcg agcaagctgg cggctgaggc ctactactgg gacccggaag
    10201 acggttgcgt cgccacgttt acccatagcc cggcatgaac atcccgataa actttcccga
    10261 cgacgagcca cccctggtgc gcctgaagtg ttacgaaccg ccgggcttaa cgctcgagct
    10321 cgaccgtgac acgctgttgc gccacttgct gttcatcggc ggcaccgggg ccggcaagac
    10381 ctccgtggtg aacggggtgc tggcccaact gttggccgcg caggtgaacg gacggcccat
    10441 tggcctgctg atcctcgacg gcaagcagga cgagtcggtg gcgcgcgtgc agaaactagc
    10501 tgcggaggcc ggccgctccg tgctggtgct cacccccgac ggggagttgc gctacgatct
    10561 gttcgcatcg ctgcgcaccc tcgacgatgt cgagccgacc gcgcagcggc tgctcctggg
    10621 cgcgggacgc ctgggcggtg acaacctcta ttggtccgac atgcgcctga ccctggtgga
    10681 tgccgccctg tcgttgctcg tggcctttgg cgttcccgtc gccttcgaaa ccgcggtcga
    10741 gttcatgcgc cgctggtttt ttggcggcac cacagacgcg gaactggtca gcaaggtgat
    10801 tcaacacacc acccgagttc tggccgaggg cgaggcccgc ttgcccgcgg cgatccggcg
    10861 caaactccag caggctttgg acaccgccgc gctgtggcgc accttggatc cgagaacgcg
    10921 gagcaacgta cagagcacgc tgctggcggc catgcgcccg ctgctgagcc gccgggccag
    10981 cctgctcttc gaggccgatg gacacgtctg ctttcgggcc gggtcggtcc tggagcaggg
    11041 cggggtggtg gttgtgtccg ccaatgccgt gaacgagccg gacctggcgc ggctgctgtt
    11101 tcgtctcgtg aagagcgatt tttaccgcgc ggtgcaggca cgttgctcgg gctcagaacc
    11161 gctggctttc ttgataatgg acgagtaccc attggccgcc agccagtccg accttcccaa
    11221 tcttcagacc ctccgctcaa aaaatgcggg agttatcgcc tgcactcagg ggttcatcag
    11281 cctggacgag gcgttggggg tgcgcattcg ccaggcgttg atcgccggtt tccagaacat
    11341 catcgccatg cggtcccacg aaatcgaaat cgatgcgctg atgttcggca tgatgggcga
    11401 gcgcgagctg gaggaaccgt cccgtggcgg tcatcgttat gaggacttga tcgattggtc
    11461 gcccggtcct ccgcgccgcc gcccggtgcc ggtgtgtccg ctgggcgcgc tggcccggct
    11521 gaaacccttc gaggcgtttg tatccctcgc caacggaacc cacccgccgc agtctgtgtg
    11581 gctcgaaccg ctctttgtcg cggtggagcc ggcgtctcct tcgatcgtgc tagccgagcc
    11641 ggccgatccg ctcgcggagg tcgtcgcccg cattcgcggc ggcgtggttg gccccgcctc
    11701 gacctcctcc atcttcgaac tgatgggccg cgtgggtggg cagatgctta tgccgcatcc
    11761 gctgtggcaa gcggcggtgg aactttgtgt tccgggcatg tcgcaggagc tgctggtcga
    11821 gcaactcacc agctttttcc gggtgcgcag cggcctgtgt ccgcgaggcc tggaaagcct
    11881 acccgcctgc tggctgcgcg cgctgccgcg cctgctggag aagcgcgcca agaaacactg
    11941 gcaggggaga atcccctttg gcctctcctc ggtcgccgag cagggcggcg ctctggtgat
    12001 ggagttcgcc caagaacacg agtgcccacg acgggaaggg gaagtgaaca gttgggatgt
    12061 cttgcgagtt cacatgaatt gccacctgta tccaagcctc tggcagccgt tgacccgccg
    12121 gcactggcgg cagctccacg ccacccgccc cgagctgcgc gcgcacctcc gggtccacgc
    12181 catcgtcgtc ggaagttcct ggttgcagta ggccggggcc aggcgtaccg cgccatgctc
    12241 gacctcgtcc cgttgcccaa ccggctttga tgcgggggcc acgacgggtg gtaaccgcct
    12301 cgaaccggcg caagtagacc ccaggtccga tttcttttcc tgaactccga ttggaattca
    12361 cccccaacaa tcccgggggc cggaggcgag ggtcggtttg cgtccctcga cttcacgatt
    12421 tcctaaggga tttgcagatc gagaaatcgg cgcgggtgac ggctaacaat ggctaacaaa
    12481 atggctaact aaccatagtg aaatcatcct gcaaaatagt tccacaaaag ggttgaatta
    12541 ccaatagaat caggctattc ttgcattcgg aatcagcgtc tcccaggccg attccgctag
    12601 tcgccccagg attaagacgg atacgattgg atacgggtgg cttaacttgg attagcggaa
    12661 actgcgaagg gccattccgg gacctaagat ctaccgtccc ttcgtctggc ttccctttca
    12721 agagcactgt ctcacgaata ttctggcgct ggatcgtcgc gtcggtgagc acgggaaatt
    12781 ggtccgcgcg ggagtaaccc tctctaaaac tgctaaaact ctaaccattc gcccaggaaa
    12841 tgacagagtt gagggtatga gctgctcgct ttatccttca cgccgatgaa acagccattg
    12901 tcgaataaag tcgcgattgt aacaggggcg agccgcggaa ttggcagagc cattgcattg
    12961 aaacttgcgg aggacggcgc ggcggtgatg gtggcggcaa ggaatgcgga gttgctaaag
    13021 gaggcgcagg ctgagatcga gagcaacgga gggaaggcgg cctggttgtc gctggatttg
    13081 cggaagccgg aggcgccagc gctgctggtg cagaatacca tcgaagtgcc tgggcgggta
    13141 tgtccccgtg atcgagttga acaagagcgt tgaagatcgg atcacctcga cggtgcggca
    13201 caacatggcg cggcggacgc atcaggtcga gctgtcggcc aagctcgtcg cggcgctcaa
    13261 gaagcacaac tggacgatcc agcgcatcgg gatggagttg gggatggacc cggacgaagt
    13321 gctgcggctc aagcagatca ccgggttggc cgaggccttc aaggacaagg aattctcgaa
    13381 gtcgtgggag tagcccgatt tggcacccga atagctgtcc aacacaaagc atgcagattc
    13441 cagaattaca tcgttactcg gtgggggagc tctatagcga aaagcggcgg cgttggccag
    13501 agggggcgca gttcctttac cggcgcgggc agttcgagtt cctcctgttc ttcaacaggc
    13561 ccagggggca ggaggtactg gatgtcagga agggggaggc ccggtttgcc ctgttgcctt
    13621 atcgcgacac cctattcttc ctgtaccgct tcggtacgaa cccgcgttgg tcggacgcga
    13681 cattttcgtg gtggctgcac gaagctgagg agcgccaggt gccgattccg ccacaggagc
    13741 tcgccgagga aagccgaatt gcgataccta tcatgctggt gggggccgac gacgggctga
    13801 taaaagcgat gcggcttgtc accttctctc cgtcgtttac ggcagagctg ttccgggcgg
    13861 tcagaaagca ggcttcgcaa ccctggtgcg gggacgaagc ctacgccaag acgctccggc
    13921 ggcctacagc ttgtggccca cgtccgacag gatggccgcg gcggcaacag ccaagtgctg
    13981 tggtggggat tgacttgatc accttgacac aatgaacaaa acgaacaaaa cgaacaccga
    14041 caacaccttt cagcaggggc tggagggcac cggtgccggg gaatgggcaa aggtgagcta
    14101 caacatgcag ttggttgtcc tcacaactgc agctactgct acgccagagg taacgctcgg
    14161 cattttaact ggatacccag cctggaggat tggccaaaca tgcggctgat accagagagg
    14221 gtcgccgagg ccgccgataa gttttgtggc acctccgtga tgtacccttc gacccacgac
    14281 atcaccccgg agatactgcc gcaggcgctc cagactctgc ggggcctgct gggcgggggg
    14341 aactcggttc tcttcgtttc aaagccgcac ctcgaagtaa tcgaggcgat ctgtcgagag
    14401 atgcccgccc ataagaccca actgcaattt cgcttcacga tcggcagtgg caacccggat
    14461 acgtgtgcgc tatgggagcc cggggcgcca gggccgaacg agcgcatagc cgcccttcgg
    14521 cacgcgttcg agctgggtta tcaaaccagc gtcagcatgg agccgatgct ggaggacaat
    14581 gccgccatgt gcgccctggt agatcgggtg accccgtttg tgacgggcac cgtgtggctc
    14641 ggcaagctca acgggggtgt cccactatac atccaggcga agccgggggt aaaagaggcc
    14701 ctccgcgcgg tccgggctgg gcagagcgac gagctcatcc tggccctgca cgatcgctta
    14761 gcccaaaacc cgaaggtacg ctggaaggac agcatcaagg aggttcttcg gaagcatggg
    14821 aggttgccga agttgcctaa tgcctctgcg aaagtggttg accccaagag gaggcaggcc
    14881 gcgctgaagg ctcatgagac aatgcgccga aggcgggcgg cgctgcaggg gacctcgggt
    14941 taatctcgtc cctgcagcgc gggtatgttt tcgacagagc tattatgcct gtcgacacat
    15001 tacatccgcc gccccctgcg gttctccatc atcttgcgct gcttgcgggt gaggtgctgc
    15061 tcgtcggagg tgacgtcggc ctcgggatcc gcctgctcct gggcgggctc gggggcccgg
    15121 gtgcgcgcgc tgtggagggc ggaggcgatg ccgaggaggg tcttgccggt ggcggcgggc
    15181 cccgtcaggc agatgagcgt cttgtcctcg ttgatcaggg cgtcgagggc ggccatggcg
    15241 gcgatggcct tgatggtggg ggcgctcact ccgtatccgc ccagggcacg ctgaccatca
    15301 gcaaggcaca ggcatccgtc atcctcgccg acctgacccc tcatgccgga tggcggacaa
    15361 ggctccgcgg aattgaaacg ccgggacgcc taagttcagc aaccacagta gttgacgaac
    15421 gtttcgacga tctgggacga aggaaacgca ctggcgtttg tgacctggtt tgtgacctca
    15481 caagacgaca ggatccgggc cgcaggtaat ctattccaaa tctggacctt tggggctcgc
    15541 gttcaaggac ttgagccagc tctcaagatc acggagatcg gctatttttt cgtcgttctc
    15601 gtctttccaa tctgaccatt ccctcttcac gaaatctgcg agtgggcctg gccggtctcg
    15661 ccacaaatcc gctgggatgg cgtcttggaa attgatcccg tgttttatgg ccgcttgaat
    15721 cccgagttcg tgattggagt ttgttcggat ccggtgcgcc caccgcaaaa gcaactgttc
    15781 ggccccaccc ttaagggtca ggtctgtgag gtgttggcgc aggatcgcga tcgaaaggtg
    15841 caggtgcttg aggtcctgcc gttctccagc ctcttgcggg agcgtcatca gattcaccgt
    15901 cttactttcc aagcaatgca gcgggtgtaa aaccttaagc gacatctgtt caaaggagat
    15961 tggcctggca agttccacta cttctgcggg agagaggccg ttggggctat gcaggaaatc
    16021 gatctcaaac tctgagccct ggatttgggt gacgattttc cccgaattca ccgtgttttc
    16081 ggctggggtg ggaaaaaagg gctctgcgtt cagcgcttgg ctgcacttca gaacgtccgt
    16141 tcggttgccc aagacatcaa gatcgaagct ggtgtagggg cgaagctcct tccaaggcga
    16201 tgaatccttc tggtaatgct cggcccagag gtttatcgcc tgtcctccaa tgatcacgcc
    16261 attcgtctgg gcaagggctt gcagaacggg tttaagttcg cgagggctgt attttctctt
    16321 cattccgtgg gcagggcttc ttcccgttct ggaaagacga actccgtggc gggagggagc
    16381 gcgcggtttc gttcctgaac cgcttccgca cctccacgtg ccacttctgc ggcgtcagca
    16441 cgcctgcgct cagcctttaa tcggcctcgc gctcttgggc tgtaaagctt gattcgccgc
    16501 ttttgtgctt cgctgagcat tggaatttgt tctggccgaa ttcacgactc cccacccgcg
    16561 tcatggaggt cgccgtctgc cacatgtctt tatggtcggg cttctggccc ggttttacaa
    16621 gggtctactc tatttccgcg ccatccccca tgtcgccatc ttcggctctc attagctccc
    16681 gagctacggc ggcgttgatg gcgcgcaact cctccaggaa tgccgttaac tcgcccacct
    16741 taggtggccg cgcgcacggt atgtcctggg tgcccttt
//
LOCUS       DATCGS010000263         5171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_617618, whole genome shotgun sequence.
ACCESSION   DATCGS010000263 DATCGS010000000
VERSION     DATCGS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617618"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1049
                     /locus_tag="VIL39_05425"
     CDS             156..1049
                     /locus_tag="VIL39_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480900.1"
                     /translation="MNSGCIPCRPSRKGSRINAPAFSLIELLVVIAVIAILAALLLPA
                     LNKAKLKAQAIQCMNNHKQLALAWRMYADDNRDVLVYASDDPGDWQVNWQELDQYAWT
                     LTHMDFDPNNQGNWDINYDMAKRPLWPYTSKNPGIYKCPADKSFLTVGGVQKPRVRSM
                     SMNLYMGGFDGTDGNWPWAHPYRIYDKLPAIDFPSKIFVFLDMREDSVNWGNFMTCME
                     GYAPNNPSQYCFDGDYPGMYHHFACGFSFADGHSELKRWRDSRTIPPLTVGANPLFEQ
                     FTASPDNQDVAWLQDHSTRLR"
     gene            complement(1104..1613)
                     /locus_tag="VIL39_05430"
     CDS             complement(1104..1613)
                     /locus_tag="VIL39_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480901.1"
                     /translation="MQKLEPPDTHYFFAAVGWLELGNAAEARTELAQVSPARQEHPDV
                     LEVRWSLAAEQECWDEALQVAQVLLRRAPKRTSGWLHQAYALRRIPDGSVQKAWEALL
                     PAFDKFPKEPTIAFNLACYACQMRQLDAARDWLQRAVAIGGKEQIHEMALKDSDLEPL
                     WHEIRQFQT"
     gene            1821..2597
                     /locus_tag="VIL39_05435"
     CDS             1821..2597
                     /locus_tag="VIL39_05435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5480902.1"
                     /translation="MKALIPILLTLGGALTAAAQIHTELIEYKHGDAVLEGCLAYDTG
                     IKGKRPGVLIVHQWKGLTDYEKKRAEMLAKLGYNVFALDIYGKGIRPQSSQEAGAEAG
                     KYKSNRDLLRARAQAGLAVLQKHELTDPKRVAAIGYCFGGTTVIELARSGADVAGVVS
                     FHGGLDSPHPEDGRNIKCKVLALHGADDPNVPPKDLAAFEDEMRQAKVDWQLVKYGGA
                     VHSFTDWNAGDNPQQGAAYNEKADRRSWEAMKQFFAEIFK"
     gene            2657..3286
                     /gene="rsmD"
                     /locus_tag="VIL39_05440"
     CDS             2657..3286
                     /gene="rsmD"
                     /locus_tag="VIL39_05440"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY5480903.1"
                     /translation="MRITGGKAARRILKVPKGLDVRPTADLVKQAVFNSLGGRVVGAR
                     VLELFAGTGALSLECLSRGATSTVCVEKSSRHAETLRHNFQLAGFPPETLQVRVQDVF
                     VALAQLAAAGERFDLILADPPYGEKNVGRRSTSLAQQLLDEVGLPRLLEPDGLLVLGH
                     TKRDTLSVPDFWQERKLLKHGDSIMRFLSAGVWKAGGDVAETEGLNLER"
     gene            3283..4053
                     /locus_tag="VIL39_05445"
     CDS             3283..4053
                     /locus_tag="VIL39_05445"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY5480904.1"
                     /translation="MKNKNEIVADWLPRYTGTPLQEFGKYILLVNFSNYVRMFAAWHK
                     VPVRGEDRPMLNATANGITIINFGMGSANAATIMDLLCAAPPKACLFLGKCGGLKKKN
                     KLGDLILPIAAIRGEGTSNDYFPPEVPALPAFSLQKAISTTIRNYQRDYWTGTVYTTN
                     RRVWEHDEDLKRYLRRIRCMAVDMETATIFITGFHNGIPSGALLLVSDQPMAPEGIKT
                     SQSDQRVTADFADMHLRVGIDSLCQLINQGLTVKHLRF"
     gene            complement(4120..4569)
                     /locus_tag="VIL39_05450"
     CDS             complement(4120..4569)
                     /locus_tag="VIL39_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY5480905.1"
                     /translation="MPAEPQATLHRILELLGFPATVEEHRMEEGILLDVKTDDSGRLI
                     GRQGQTLADLQYITNRLLFQQDPSVPKALVDVGGYRAQAREALVKKAKEAAEKVRRWG
                     DVVELEPMSAFDRRIIHQALKDDPSIETHSVEVEGTDRKAILLRPKR"
     gene            complement(4632..>5171)
                     /gene="yidC"
                     /locus_tag="VIL39_05455"
     CDS             complement(4632..>5171)
                     /gene="yidC"
                     /locus_tag="VIL39_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY5480906.1"
                     /translation="YKDDPVKMNKKTMEFMKENKVSPLGGCLPMLLQIPVFFGFFSMI
                     QSAVELRGASFLWIGDLAQTDTLFLIPGLGFIPFFGIVGVGLPFNLLPLIMGATMLWQ
                     AQMTPPSPGMDPTQQKIMRYMPLMFLAFLYNYSAGLTLYWTVQNLLTILQTKLTRTAP
                     AATVLAKVPGLTPPAKKRK"
BASE COUNT         1027 a         1529 c         1596 g         1019 t
ORIGIN      
        1 tatcaagcgc gacggcttgt ttggatacgg gaagagcgcg atgtccgcag gacgcagttc
       61 gtagtccgtt gggcttcggg cgaggacgcg ccgtgcgcat ggcagattga cccaacccgc
      121 gccggggcat acattataga aagcgctggt ggaaaatgaa ctcaggctgc attccatgcc
      181 gtccgtcccg taaggggtca agaatcaacg ccccagcatt ttcgctgatc gagctgctgg
      241 tggtcatcgc cgtcattgcc atcctggctg ccctgcttct gccggcgttg aacaaggcca
      301 aactgaaggc ccaggccatc cagtgcatga acaaccataa acagttggct ttggcctggc
      361 ggatgtatgc cgacgacaac cgcgacgtgc tggtctatgc cagcgacgat ccgggcgatt
      421 ggcaagtcaa ctggcaggag ctggaccagt atgcctggac gttgacgcac atggattttg
      481 acccgaacaa ccagggaaac tgggacatca actatgacat ggccaaacgc ccgctgtggc
      541 cctacacgtc gaagaaccca ggcatttaca agtgcccggc cgacaagtcg tttctgacgg
      601 tgggcggggt gcagaagccg cgcgtgcggt caatgtccat gaacctttat atgggcgggt
      661 ttgacggcac cgacgggaat tggccctggg cgcatcctta tcgcatttat gacaagctcc
      721 cggccattga tttcccctcc aagatctttg tattcctgga catgcgcgag gacagcgtga
      781 actgggggaa cttcatgacc tgcatggagg gctatgctcc aaacaacccc tcccagtatt
      841 gcttcgatgg cgactaccca gggatgtatc accactttgc gtgcggcttc tccttcgccg
      901 acggccattc cgagctcaag cgatggcgag actcacgcac cataccaccg ctgacggtcg
      961 gggcaaatcc attgtttgaa cagtttacgg cgtctcccga taatcaggac gtggcctggc
     1021 tgcaagatca ctcgacccgc ctgagatgat tctggaacga atcaagcggt cttggtgcgg
     1081 tgacgccgtt atccaggctc gttttaggtc tggaactgcc ggatttcgtg ccaaagcggt
     1141 tccaggtcgg agtccttcag cgccatctcg tgaatctgct ctttgccgcc gattgccacc
     1201 gcgcgctgaa gccagtcccg ggcggcatcc agttgccgca tctggcaggc gtaacacgca
     1261 agattgaagg caatcgtcgg ctccttgggg aacttgtcga acgccggcag cagcgcctcc
     1321 caggccttct gaacactccc gtccgggatg cggcgcaagg cgtacgcctg atgcagccag
     1381 ccggacgtgc gcttcggggc gcgacgcagc agaacttgag cgacctgcag cgcctcatcc
     1441 caacactctt gctccgccgc gagcgaccag cgcacttcca acacatccgg atgctcttgc
     1501 cgggctgggc tgacctgagc caattccgtc ctggcctcgg ccgcgttgcc cagttccaac
     1561 cagcctacgg cggcgaagaa ataatgcgtg tcgggcggct caagcttttg cattgcgtga
     1621 taatagctta gcatcactgg cgtggtgtca agggcgaccg tgcatgcgac agcatttctc
     1681 actatggcca aagccctggg aaaacaggtg gttgccgcgc ccaggctctg gggtggcttt
     1741 agggtgactt tgggcgcgta agggcttaga cagccaatcg cagttggcat tttcgttaat
     1801 cctggtttat ctgtgctcgt atgaaagcat tgattcccat actgctcact ttaggcggcg
     1861 cgctgacggc agcggcgcaa atccatactg agttgatcga gtacaaacac ggcgatgcgg
     1921 tcctggaagg ctgcctggcc tacgacacgg ggatcaaggg caagcgtccc ggcgtgctca
     1981 tcgtgcatca gtggaagggg ctgacggatt acgagaagaa acgcgccgag atgctcgcca
     2041 agctcggcta caacgtcttc gcgctggaca tctacggcaa ggggattcgc ccacagtcaa
     2101 gccaggaagc cggcgctgag gcgggcaaat acaaaagcaa ccgtgacctg ctgcgtgccc
     2161 gggcccaggc cggcctggct gtcctgcaaa agcatgaact gacggacccg aagcgggtcg
     2221 cggccatcgg ctattgtttt ggcggcacga cggtgatcga gttggctcgc agcggggcgg
     2281 acgtcgccgg tgtggtcagc tttcatggcg gcctggattc gccccatccg gaggacggca
     2341 ggaacatcaa atgcaaggtc ctggccctgc acggcgcgga tgaccccaac gtgccaccca
     2401 aagacctggc cgccttcgag gacgagatgc gccaggccaa agtggactgg caactggtca
     2461 aatacggcgg tgcggtccac agttttaccg actggaacgc gggggataac cctcagcagg
     2521 gcgccgccta caatgaaaag gccgaccgcc gctcgtggga ggcgatgaag cagttcttcg
     2581 cagagatctt caagtgaacc tctcctgcag acattttcat tcgtgctatc tgtgaaaccg
     2641 ccagctagag tttcccatgc gcatcacagg tggaaaagcc gctcgccgga ttctgaaagt
     2701 gccgaagggc ctggacgtgc gccccacagc agacctcgtc aagcaggccg tcttcaacag
     2761 cctcggcggc cgcgtggtgg gcgcgcgggt gctggagctg ttcgccggca cgggcgcgct
     2821 cagcctcgaa tgcctcagcc gcggcgcgac ctcgacggtg tgcgtggaga aatcatcgcg
     2881 ccacgcggag acgctccgcc ataacttcca gcttgccggt tttcctcccg aaaccttgca
     2941 ggttcgggtg caggacgtgt tcgtcgccct ggcccaactg gcggcggcgg gcgagcggtt
     3001 tgacctcatc ctggcggacc cgccttatgg agaaaagaac gtcggccgcc gctcgacttc
     3061 cctcgcccag caactgctgg atgaggtcgg gttgccgcgg ctccttgagc ccgacggctt
     3121 gctcgtcctg ggccatacaa aacgcgacac gctgagcgtg cccgactttt ggcaggaacg
     3181 gaagctgctc aagcacggcg acagcatcat gcggtttctg agcgcgggcg tgtggaaggc
     3241 gggaggcgat gtcgcggaaa ccgaagggtt gaacctggag cgatgaaaaa caagaatgag
     3301 atcgtggccg actggctgcc tcgttatacg ggcacgccgc tgcaggagtt cgggaagtac
     3361 atcctgctgg tgaacttcag caactacgtg cgcatgttcg ccgcgtggca taaggtaccc
     3421 gtgcgcggcg aggaccgccc gatgctcaat gccacggcca atggcatcac catcatcaac
     3481 ttcggcatgg gcagcgccaa cgcggcaacc atcatggacc tgctgtgcgc cgcgccgccc
     3541 aaagcctgct tgttcctggg caagtgcggc gggttgaaga agaagaacaa gctgggcgac
     3601 ctgatcctgc cgatcgcggc gatccgcggc gagggcacct cgaatgatta ctttccgcct
     3661 gaggtgccgg cgttgcccgc cttcagcttg cagaaggcca tctcgacgac catccgcaat
     3721 tatcagcgcg actattggac cgggacggtc tataccacca accgtcgcgt gtgggagcac
     3781 gatgaagacc tgaaacggta tttgcgccgc atccgatgca tggccgttga catggagacg
     3841 gcgaccattt tcatcaccgg gttccacaat gggatcccca gcggcgcgct cctgctggtc
     3901 tcggatcagc ccatggcgcc ggagggcatc aagacctcgc aaagcgatca gagagtaacc
     3961 gctgattttg cggacatgca cctgcgcgtc gggattgatt cgctttgcca gctcatcaac
     4021 caggggctga cagtgaaaca cctgcggttt tagagcacag cccctccttc ttcacgccac
     4081 gaggccagag accatccacc agcaatccac cgcccggctt tagcgcttcg ggcggagcag
     4141 aatcgctttc ctgtctgtgc cttcgacctc gacgctgtgg gtctcgatgc tcgggtcgtc
     4201 tttgagcgcc tggtggatga tgcggcggtc aaaggcgctc atcggctcca gttccaccac
     4261 gtcgccccag cgccggactt tctcggcagc ttccttggcc ttcttgacga gcgcctcgcg
     4321 ggcttgcgcg cggtagccgc ccacgtccac cagcgctttg ggcactgacg ggtcctgttg
     4381 gaagagcagc cggttggtga tgtattggag gtcggcgagg gtttggccct ggcgtccgat
     4441 gaggcggccc gagtcgtcgg tcttcacgtc gagcaggatg ccttcttcca tgcggtgctc
     4501 ttcgacggtg gcagggaagc cgaggagttc cagaattctg tgcagcgtgg cttgaggttc
     4561 agcaggcata cgagagttga tagttgatgg ttgatagttg atagctgaaa gctgccgttt
     4621 ccattggccc tctatttcct ctttttcgcg ggcggtgtca atcctggaac tttggcgagg
     4681 acagtggctg ccggagcggt tcgggtcagc ttggtctgca ggatggtcag caggttttgc
     4741 accgtccaat agagggtcaa cccggccgag tagttgtaga ggaaagccag gaacatcagc
     4801 ggcatgtagc gcatgatctt ctgctgggtc gggtccatgc cgggcgaggg gggcgtcatc
     4861 tgcgcctgcc agagcatcgt tgctcccata atcaatggca ggaggttgaa gggcaagccg
     4921 acccccacaa tgccgaagaa cggaatgaag cccaaaccgg ggattaggaa cagcgtgtcg
     4981 gtttgagcca ggtccccaat ccacagaaag ctggcgccgc gcagctcgac ggcgctttga
     5041 atcatcgaaa agaagccgaa aaagacggga atctgcagga gcatcggcag gcagccgccc
     5101 agcgggctga ccttgttctc cttcatgaac tccatcgtct tcttgttcat cttcaccggg
     5161 tcatctttgt a
//
LOCUS       DATCGS010000264         4126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_218758, whole genome shotgun sequence.
ACCESSION   DATCGS010000264 DATCGS010000000
VERSION     DATCGS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218758"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2515)
                     /locus_tag="VIL39_05460"
     CDS             complement(<1..2515)
                     /locus_tag="VIL39_05460"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY5480907.1"
                     /translation="MTKLLRLSTIRRSVSSRHFVVAWLLLAAVASVAPFSASAVAVGQ
                     LRCEYLKDPLGIDTPQPRLSWILRADKGSARGQAQTGYQILVARNRNELEANQGDLWD
                     SGKVTSDKSIQVRYAGKALISEQECFWKVRVWDEQGKPSAWSEAARWSMGLLNPSDWH
                     ANWIGLDEPAESSTGKNVLGDAQWVWFPEGQPEKAAPIGTRCFRCAVTVPTDRIVKRA
                     TLFFTADNSGEMYVNGQKAGTASDFHSATKADVTKLLHPGKNVLAASVKNDGADPNPA
                     GLIALLRVEFAQGAPLVVLTDSSWKSENREMAGWKGAGFDDAGWVAAQKLGPAGMAPW
                     GKISGPEDRRLPARMLRREFAVEKKVQRATAYVCGLGLCEFYLNGKKVGDHVLSPGLT
                     DYTKRALYVTFDVTRQLKRGANVAGIMLGNGRFYAPRVSVPTGTTSYGYPKLLFQMHV
                     EYRDGTSAEVVSDGAWKLSAEGPIRANNEYDGEDYDARLEMPGWSAPGFDGAKWQSAQ
                     AVTAPGGKLAAQMIEPIRVTETLKPIAVTQPQPGVWVFDLGQNMVGWCRIKVSGPGGT
                     EVKLRHAETLKPDGTLYLDNIRGAKVTDTYTLKGKGTEVYEPRFTYHGFRFVEVTGYP
                     GRPSLASIEGQVVHDDLQNAGDFACANPLLNRIYKNIVWGVSGNYRSISTDCPQRDER
                     QGWLGDRSAEAKGETYLFNTAALYAKWLQDMADAQKENGSVSDVCPAYWPFYSDNVTW
                     PSSTVIIPGALREQFADEGIIASHYDSAKKWMDHMNTFVANGIIERDSYGDWCVPPED
                     PKLIHSNDPARKTDKAVLATAYFYHDALLMARYATLLGKA"
     gene            2955..3560
                     /gene="thrH"
                     /locus_tag="VIL39_05465"
     CDS             2955..3560
                     /gene="thrH"
                     /locus_tag="VIL39_05465"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEY5480908.1"
                     /translation="MKQSIVTLDMEGVLTPEIWIAVAEKTGIKELRRTTRDEPDYDKL
                     MRGRLKILDQHALKLSDIQQVIGTLRPLEGAKAFLDELRSLVQVIILSDTFEQFATPL
                     LRQLGWPTLLCHRLVVEKDRIVDYKLRVPEQKRRAVAAFKLLNYYVISGGDSFNDTAM
                     LSEAHVGFLFRAPDNVKQQFPQFKAVDEYADLMKLIKQAMG"
     gene            complement(3603..>4126)
                     /locus_tag="VIL39_05470"
     CDS             complement(3603..>4126)
                     /locus_tag="VIL39_05470"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5480909.1"
                     /translation="IAADAVQKLFNRPSFRVYTSTDRLGVELGGALKNVIAIAAGVAD
                     GLGFGDNSKAALVTRAIVEIRRLGVACGAQAETFTGLSGLGDLMVTCFSKHSRNRGFG
                     ERVGRGEKPAAIVASMLAVAEGYPTARSAFRLARKHHVSTPVIDEVHAMLYEGKNVAQ
                     AVRDLMSRDLKPE"
BASE COUNT          753 a         1314 c         1243 g          816 t
ORIGIN      
        1 cggctttgcc aagcaaggtc gcgtagcggg ccatcaatag cgcgtcgtgg tagaaatagg
       61 cggtggcgag cacggctttg tcggtcttgc gcgcggggtc gttcgagtgg atcagcttcg
      121 gatcctcggg cggcacgcac caatcaccgt aggagtcccg ctcgataatg ccattggcga
      181 cgaaggtgtt catgtggtcc atccacttct tggcactatc atagtggctc gcaatgatgc
      241 cttcatcggc gaactgctcg cgcagggcgc cgggaatgat caccgtgctg ctcggccagg
      301 tgacattgtc cgagtaaaac ggccagtagg ccgggcacac gtcggacacg ctgccgttct
      361 ccttttgcgc gtcggccatg tcctggagcc acttggcgta gagcgcggcg gtgttgaaga
      421 ggtaggtctc gcctttcgcc tcggcggaac ggtcgcccag ccagccctgg cgctcgtcgc
      481 gctgcgggca gtcggttgag atgctccggt aattgccgct gaccccccag acgatgttct
      541 tatagatgcg gttcagcagc gggttggcgc aggcaaagtc acctgcgttc tggagatcat
      601 catgcaccac ctgtccttcg attgaggcca ggctgggtct tccgggatag ccagtcacct
      661 cgacgaagcg aaaaccgtga taggtaaagc gcggctcata gacctccgtg cctttgccct
      721 tcagcgtgta ggtgtccgtc actttcgccc cgcggatgtt gtcgaggtaa agtgtgccgt
      781 cgggctttag cgtctcggcg tggcgcaact taacctcagt cccgcccggg ccggacactt
      841 tgatgcggca ccagccgacc atgttttggc ccaggtcgaa aacccacacg ccgggctgcg
      901 gctgcgtgac ggcgatcggc ttgagcgtct ccgtgacgcg aattggctcg atcatttgcg
      961 ccgcgagctt gccgcccgga gcggtgactg cctgggcaga ctgccatttc gcaccatcaa
     1021 agcccggcgc gctccaaccg ggcatttcca gccgcgcgtc ataatcttcg ccgtcgtatt
     1081 cgttattggc acggatcggg ccctcggcgc tcagtttcca ggcgccgtcg ctgaccactt
     1141 ccgccgaggt gccgtcccga tactcgacgt gcatttggaa cagcagtttg ggataaccat
     1201 aactcgtggt gccggtcggc acggagacgc gcggcgcgta aaagcggccg ttgcccagca
     1261 tgatgccggc cacgttcgcg ccccgcttga gctgcctggt cacgtcgaac gtaacgtaga
     1321 gggcgcgttt agtataatcc gtcaggcccg gggacaagac gtggtccccg accttcttgc
     1381 cattgagata gaactcacac aagccgagcc cgcagacgta ggccgtcgcg cgctggactt
     1441 tcttctcgac cgcaaactcc cggcgcagca tccgggcggg caaccggcgg tcttccgggc
     1501 cgctgatttt gccccacggc gccatgcccg ccgggccaag cttttgcgcg gctacccaac
     1561 ccgcgtcgtc gaagcccgca cctttccatc cggccatctc cctgttctcg ctcttccagc
     1621 tcgaatccgt caggaccaca agcggtgcgc cctgggcaaa ttcaactcgc agcaaggcaa
     1681 tcaagcccgc gggattcgga tccgccccgt cgttcttcac agaggcggcg agaacattct
     1741 tgccgggatg caggagcttg gtcacgtcgg ccttcgtcgc ggagtggaag tcgcttgccg
     1801 tcccggcttt ctgcccgttg acatacattt cgccgctatt gtcagccgta aagaacagcg
     1861 ttgcgcgctt cacgatgcga tccgtgggca cggtgaccgc gcaccggaag cagcgtgtgc
     1921 caatcggcgc cgccttctcc ggctggccct ctgggaacca gacccattgc gcatcgccga
     1981 ggacgttctt cccggtgctg ctttccgccg gctcgtccaa cccgatccaa ttcgcgtgcc
     2041 aatccgacgg gttgagcagt cccatgctcc accgcgccgc ttcgctccaa gccgacggct
     2101 tgccttgctc gtcccacacc cgcaccttcc agaagcattc ctgctctgag atcagcgcct
     2161 tgccggcgta acggacctgg atggatttgt ctgaggtgac cttgccgctg tcccacaagt
     2221 cgccctggtt ggcctcgagt tcgttccggt tgcgggcgac caggatttgg tagcccgtct
     2281 gggcctgacc ccgggcggag cctttgtccg ccctcagaat ccaactcaag cgcggctgcg
     2341 gcgtgtcaat ccccagcgga tctttcagat actcgcagcg caattgcccg acggcgacgg
     2401 ccgatgcgga aaagggcgcc acgctggcta cggcggccaa caacagccag gcaaccacaa
     2461 aatgccgtga agaaaccgaa cgtcggattg tcgagagtcg gagtaattta gtcatagggt
     2521 catctagttc atactgtatt gcagcgcgca gcttcacata tcgccctgca tgcgtccagc
     2581 ccgcgtgagg tctggtataa atccgaaggg cgaggggcga accaacagcc cagcagccct
     2641 cctgaccaag cccttgtcgg atttcgggtt tcgggcttct ttcggccttc gggtttcggc
     2701 cttcggattt cagggccgcg ggcttggccg attgatctac cgcttgggcg gcggcgggcc
     2761 attggtgtca ggcgggtcct ggacgaactc cttcaggaaa ctgtcaacca cacttggtcg
     2821 gaatcgggag cttgatgcag ccctggccaa ccaaggtgtg gcgggccgct ccaacctcgt
     2881 gtgccgtaaa tcgcccatcc gaacgtccat cccaacgctg gacacttccg tcgcattttg
     2941 aaaaactccc gtcagtgaaa caatcgattg taaccttgga tatggaggga gtgctgactc
     3001 ccgagatttg gattgccgtg gcggagaaga ccggcatcaa agagctgcgc cgcaccactc
     3061 gcgacgaacc ggactatgac aagctgatgc gcggccggct gaaaatcctc gaccagcacg
     3121 ccctcaagct ctccgacatc cagcaggtca tcggaaccct gcggccgctc gaaggggcga
     3181 aggcatttct cgatgaactt cgctcgctgg tgcaggtcat catcctgtcc gacacgttcg
     3241 aacagttcgc cactccgctg ctgcggcaac ttggctggcc cactttgctc tgccatcggc
     3301 tggtggtcga aaaagaccgc attgtggatt acaagctgcg cgtgcccgaa caaaagcgac
     3361 gggcggtagc ggccttcaaa ctcctcaatt actacgtcat ctcgggcggc gattccttca
     3421 acgacacagc catgctgagc gaggcgcatg tcggcttcct gttccgcgcg ccggacaacg
     3481 tgaagcagca attcccccag ttcaaggcgg tcgatgagta tgccgacctg atgaagctca
     3541 tcaaacaggc gatgggctaa ggagaagctg gcgctgcggt cggcggtatg agcctcgggg
     3601 gattactccg gcttcagatc ccgggacatg aggtcgcgga cggcttgggc aacgttcttg
     3661 ccctcgtaga gcatggcatg gacttcgtca attacgggcg tgctgacgtg atgcttgcgg
     3721 gccagtcgaa aggccgaacg ggccgtaggg tagccctcgg ccaccgccaa catgctggcg
     3781 acgatggcag ccggcttttc tccacggcca acgcgctcgc caaagccgcg gttgcggctg
     3841 tgcttggaaa agcaggtcac catcaaatcg cccaggccgc tgagaccggt aaacgtttcg
     3901 gcctgggcgc cgcaagcgac accgaggcgg cggatttcaa caatcgcacg ggtcaccaat
     3961 gcggccttgg agttgtcccc gaatcccagc ccgtccgcga cgcccgccgc gatggcaatg
     4021 acgttcttca aggcgccccc cagctcgacg ccgagccggt ccgtgctggt atagacgcga
     4081 aaagaaggcc ggttaaagag cttttgcacc gcatcggcgg cgatca
//
LOCUS       DATCGS010000265         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_419667, whole genome shotgun sequence.
ACCESSION   DATCGS010000265 DATCGS010000000
VERSION     DATCGS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419667"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VIL39_05475"
     CDS             <1..1488
                     /locus_tag="VIL39_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY5480910.1"
                     /translation="WPTKPDGTRFQADGGYNPEMKQAQLRELLTQYGPIEFIWFDHAV
                     GDGGLSHADTVKLVKSLQPGCFVGFNTGEQEGADIRLGEEGRPGALEDQAAAGPHMQT
                     KAASTYRLAEFTYPIQPKHEGGAMWFYSLPKHDQLCRPAEQIYADYLGAVRYGNLFSL
                     DVGPDYAGRLREIDVKTLRQVGQYIRGEAKPPVASGSTSSQGEAGDTCAQPSPEEMAK
                     AAGVTLPHQPWHLANIWWEFEKPTEHFESLEVDVTIDRDVPSTYNLYVSPCGLARIND
                     KDFYGGLQSNINGWANRTNHTRVHPGKGAIFSRWSSDKKTPVSLDNVRTAGPDCLVES
                     AGYEGEFCSVRRPFAWTKGTYTYQIVKGATEVANGQTNTWFTCRVKDPQGAVHEIGSL
                     RFEGSDFTFWDRHSAFVEVYSTEKIPRAGIPKVNITFGWPRLNGHEPSLKRAHAYYPS
                     KTGPAAPDCAWIKADGQSVRVEIGPIFQRDEAKRRHDLPLKRQGA"
     gene            complement(1540..>3291)
                     /locus_tag="VIL39_05480"
     CDS             complement(1540..>3291)
                     /locus_tag="VIL39_05480"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEY5480911.1"
                     /translation="GKRFEHGIGTHANSIFRIALGGKAERFTATVGVDDEVGDKGSVV
                     FKVIGDGKTLWESGVLHGGDAAKNASVALDGVKVLVLTVGDAEDDMNYDHADWADARI
                     VMTEGKPEAVAPVREPAVVLTPKPSPKPRINGAKVVGVRPGSPFLFTVPATGESPVTI
                     SATKLPAGLQLDPQSGCITGVLKEKGTYVVTLHAQNAKGTAERALKIICGDTIALTPP
                     MGWNSWNCFACDVTEANVRAAADAMVASGLIRHGWTYINIDDCWEAGRDADGNVLSNQ
                     KFPDMKALTDYVHSKGLKIGLYSSPGPKTCAGHEGSYKHEEQDARRYGEWGFDYLKYD
                     WCSYGGIVPNPDHDGLMKPYQIMRAALDKAPRDILFSLCQYGMGNVWEWGAQVGGNCW
                     RTTGDITDTWSSMAGIGFGQAGHEKFVGPGHWNDPDMLVVGYVGWSAKVRLTRLTPSE
                     QYTHISLWCLLCSPLLIGCDMTKLDDFTLNLLTNDEVLDVSQDPLGRQAGRIAKNGSL
                     EVWAKDLEDGSKAVGLFNRGEDGATITVKWSELGISGPQNVRDLWRQLDLGKFPGQFQ
                     TEVSRHGVVLVKVTPAR"
BASE COUNT          607 a         1064 c         1008 g          612 t
ORIGIN      
        1 tggccgacca agccggacgg cacgcgcttc caggcggacg gaggctacaa ccccgagatg
       61 aagcaggccc agctccgcga gctgctgacg caatatgggc cgatcgagtt catctggttc
      121 gatcacgccg tcggcgacgg cggtctcagc catgccgaca cggtcaagct cgtcaaatcg
      181 ctgcaacccg gctgctttgt cggtttcaac accggcgagc aagaaggggc cgacatccgc
      241 ctgggcgagg aaggccggcc aggcgctttg gaagaccagg cggcggccgg tccccacatg
      301 cagaccaagg cggcttctac ctaccggctg gctgagttta cctacccgat ccagccaaag
      361 cacgagggcg gggccatgtg gttctactca ttgccaaaac acgaccaact gtgccgtccg
      421 gcggagcaga tctacgccga ttaccttggc gcggtgagat acggcaacct gttctccctc
      481 gatgtcggtc cggactacgc aggccggctg cgcgagattg atgtcaagac gctgcgccag
      541 gttggccaat acatcagggg tgaagccaag ccacccgtcg cttcaggctc cacctccagt
      601 cagggcgagg ccggggatac ctgcgcccaa ccctcgcccg aagagatggc caaggccgcc
      661 ggcgtgacct tgccgcatca gccgtggcac ctggccaata tctggtggga attcgagaag
      721 cccacggagc acttcgagtc gctcgaggtg gacgtgacca ttgatcggga cgtgccctcc
      781 acttacaacc tctatgtctc gccctgcggt ctggcccgca tcaacgacaa ggacttctac
      841 ggcggcctcc agtccaatat caatggctgg gccaatcgca ccaaccacac ccgcgttcac
      901 cctggcaaag gcgcgatatt ctcgcgctgg tcgagcgaca agaagactcc cgtgagcctg
      961 gacaacgtcc ggaccgccgg gccggattgc ctggtcgaga gtgcgggtta tgaaggcgaa
     1021 ttctgcagcg tgcgccgccc ctttgcctgg accaagggga cctacaccta ccaaattgtg
     1081 aaaggcgcca ccgaggtggc aaacggccag accaacactt ggttcacctg ccgcgtaaag
     1141 gacccccagg gtgccgttca tgagatcggc agcctgcggt tcgagggcag cgatttcacg
     1201 ttttgggatc gccactcggc cttcgtcgaa gtctatagca cggagaagat tccgcgcgcc
     1261 ggcatcccca aggtcaacat caccttcggc tggccgcggc tcaacgggca tgagcccagc
     1321 ctcaaacggg cgcacgccta ttatcccagc aagaccggcc ccgctgcgcc cgattgcgcc
     1381 tggatcaagg ccgacggcca aagcgtgcgc gtcgagatcg ggcccatttt ccagcgcgac
     1441 gaagccaagc gccgccacga cctgccgctc aaacgccagg gtgcctgacc gtcggcaccc
     1501 tggttcgagg aggatgctgc tccgcctcgc ccatcctgcc tatcgcgcag gcgtgacttt
     1561 gaccagcacg acgccgtggc gggacacctc cgtttgaaat tggccgggaa acttgccaag
     1621 gtccaactgg cgccagaggt cgcggacgtt ttgcgggccg gagataccca gttcggacca
     1681 cttcacggtg atcgtggcgc catcttcacc acgattgaac aggccgaccg ccttggagcc
     1741 gtcctcgagg tccttggccc agacttccaa cgagccgttc ttcgcgatgc ggccggcctg
     1801 acggccgagg gggtcctggc ttacgtcaag cacttcgtcg ttggtgagca ggttgagcgt
     1861 gaagtcgtcc agcttcgtca tgtcgcagcc gatgagcagc ggcgagcata gcaggcacca
     1921 gaggctgata tgggtgtatt gctcgctggg cgtgaggcgg gtgaggcgga ctttggcgct
     1981 ccagccgacg tagcccacca ccagcatgtc cgggtcgttc caatgcccgg ggccgacgaa
     2041 cttttcgtgg cccgcctgcc caaagccgat gcccgccatg ctcgaccagg tgtcggtaat
     2101 gtcgccggtg gtgcgccagc aattgccacc gacctgggcg ccccattccc atacgttgcc
     2161 catgccgtat tggcacaggc tgaacaggat atcacgcgga gccttgtcga gcgcggcacg
     2221 catgatctgg tagggtttca tgaggccgtc gtggtccgga ttgggcacga tgccaccata
     2281 gctgcaccag tcgtatttta ggtaatcaaa tccccactcc ccgtaacggc gggcatcctg
     2341 ttcctcgtgc ttgtagctgc cctcgtgtcc ggcgcaagtc ttcggaccgg gcgaggagta
     2401 cagtccgatc ttcaagccct tgctgtggac gtaatccgtc agagccttca tgtcggggaa
     2461 cttctggttg gagagcacgt tcccatcggc gtccctcccg gcttcccagc agtcgtcgat
     2521 gttgatgtag gtccagccgt gccggatcaa gccgctggcg accatcgcgt cagccgcggc
     2581 gcggacgttc gcctcggtga cgtcgcatgc gaaacaattc cagctattcc agcccatcgg
     2641 cggcgttagg gcgatggtgt caccgcaaat gatcttgagc gctcgttcgg cggtgccttt
     2701 cgcgttctgg gcgtggagcg tgacgacata ggtgcccttt tccttaagca cgccggtgat
     2761 gcagccgctc tgaggatcga gttgcagccc ggcgggcagt ttcgtggcag aaatggtcac
     2821 agggctctcg ccggtggccg ggacggtgaa gaggaaaggt gagccgggtc ggacaccgac
     2881 cacctttgcg ccattaatgc gaggcttagg cgaaggtttc ggcgtcaata caactgccgg
     2941 ctcgcgcacc ggtgcgacgg cttcgggctt gccttcggtc atcacaatcc tggcgtcggc
     3001 ccagtctgca tggtcgtagt tcatgtcgtc ctcggcatcg cccaccgtca gcacgagcac
     3061 cttaaccccg tcaagggcta cggaagcatt tttggccgcg tcgcccccgt ggaggacgcc
     3121 gctttcccag agagtcttgc catcgccgat gaccttgaac acgacgctac ccttatcgcc
     3181 gacttcgtca tcaaccccga ctgtggctgt gaaacgctcc gccttgccgc cgagcgcgat
     3241 acgaaagatg ctgttggcat gggtcccgat gccatgctcg aagcgcttgc c
//
LOCUS       DATCGS010000266         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_123233, whole genome shotgun sequence.
ACCESSION   DATCGS010000266 DATCGS010000000
VERSION     DATCGS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123233"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1230)
                     /gene="phoU"
                     /locus_tag="VIL39_05485"
     CDS             complement(154..1230)
                     /gene="phoU"
                     /locus_tag="VIL39_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5480912.1"
                     /translation="MDLIRSKVTEMAGLGERALKASLRALLERNRQLAYSVILRDQYI
                     DEVETELNRLCLEFLVRQQPVAGQLRFVFTTIQINKELERIGDYAESIARQVLAVSAL
                     EPQPPLTRFVELGNLSVAMLRDAVRSFLEEDPDLAWRTMATEEQGNTLRSAINAELMD
                     LSRKGQLPTAGLNPLMTIARRFERVTDQAKNLCEEVLYMCTGEVTKHKGGDAFRILFF
                     DSSNACLSQMAEGIGNAMSLPRFVFSSAGVIPQPVDERAVKFMAQKGIDLSRQTSKSL
                     EQVPQWEQYQVVIALSAQAREALPIRAKKTICLTWPVPDPAQIEATPDAKVAAYESAF
                     QFLQSHIQELVGAILEQPQTEQKL"
     gene            1477..2106
                     /locus_tag="VIL39_05490"
     CDS             1477..2106
                     /locus_tag="VIL39_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY5480913.1"
                     /translation="MFSLQKLLGKEDKFFTLLEASAEEARGSVQALVKLSKTLDNPPP
                     AEDFAYARQKGKQITREISAAVYTTFITALEREDIEELSNALYKIPKMIDKFATRLLA
                     TPQYVRGIDFSRQIGLLERATDIVLELVKSLRQGMDLEKVKDLNDKLQFLESEADTHM
                     MSLYKDLFSGKHEPVQVIVLKDLYELLEKVIDRCRNAGNVIANIALKNS"
     gene            2113..3192
                     /locus_tag="VIL39_05495"
     CDS             2113..3192
                     /locus_tag="VIL39_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY5480914.1"
                     /translation="MTLLLTVIVVALVFEFINGFHDTANSVAAVVGTRVLTPTQAIGL
                     AAVTNLCGALAGTAVATTVGQGLVDAHCVTGVTLICGLLGAILWNLITWRLGLPSSSS
                     HALIGGLIGSAVASAGNNWNVVIWSAPVAGKPWYAWGGVLYKVIIPMFSSPILGFVVG
                     FLWMTLLYILLSRCRPSSINKAFSKLQILSAAFLGFSHGSNDAQKTMGIVALALFSAT
                     TIGDLTHLPSWLQFLHTPEFKIALWVKLACAFTMAAGTATGGRRIIKTLGRKVSRLQP
                     INGFAADTTSATVLMTTAAFGMPVSTTHAVSTSIMGVGTARNHKAMKWHVVESIIWTW
                     VMTLPATGLLAYALRRIFLALGSGA"
     gene            3313..>3915
                     /locus_tag="VIL39_05500"
     CDS             3313..>3915
                     /locus_tag="VIL39_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY5480915.1"
                     /translation="MTLLLTVILVALVFEYINGFHDTANSIATVVATKVLTPRQAVLL
                     AACTNLLGALWGTAVARTIASGLIDTRLVLLTSDVMICALLGAIIWNLITWWFGLPSS
                     SSHALIGGLCGSALAASNNNWQVIIWSQPNPQHWYQGKGMLWKVIVPMVTSPMAGLIL
                     GFIVMALLYLLLQRARPNWVNRFFGRAQMLSAGAMGIMHGT"
BASE COUNT          738 a         1194 c         1145 g          838 t
ORIGIN      
        1 agcctttgtt ctggatggtc tgctgcttgg cggcatcacc gccgccgctg ggttcgcttt
       61 tgccacagcc ggtttggagc agacacacag cgaggactgc cgccgtggcc gccgcgttga
      121 gtttgaggca ggatgaatat cgttggtatt ggttcatagc ttttgttctg tttgtggttg
      181 ttctagtatc gcgccgacca gctcctggat atgggattgc aggaactgga aagccgattc
      241 gtaagcggcc accttggcgt cgggcgtggc ctcgatttgc gccgggtccg ggactggcca
      301 ggttaggcag atggtcttct tagcacggat gggcagggcc tctcgggcct gggcgctgag
      361 ggcaatgacc acctgatact gctcccactg cggcacctgt tcgagcgatt tgctggtttg
      421 ccgggaaagg tcaatgccct tctgggccat aaacttcacc gcgcgctcat ccactggttg
      481 aggtataacc ccggcgctgc tgaagacgaa ccgaggcagg ctcatggcat tgccaatgcc
      541 ttcggccatc tggctcaagc aggcgttgct tgagtcgaag aagagaatgc ggaaggcgtc
      601 gccgcccttg tgcttggtaa cttctcccgt gcacatgtag agcacctcct cgcagaggtt
      661 cttggcctgg tcggtcacac gctcgaagcg gcgcgcgatg gtcatcagcg ggttcaagcc
      721 agccgtcgga agctggccct tccggctgag gtccatcaac tctgcgttga tagcgctgcg
      781 cagcgtgttc ccctgttcct cggttgccat cgtccgccac gcaagatcgg gatcctcctc
      841 caggaaggac cggactgcgt ctcgcagcat ggcgacggat agattgccga gttcgacgaa
      901 tctggtgagc ggcggctggg gttccagcgc gctgaccgcc agcacctggc gggcgatact
      961 ttccgcataa tcgccgatgc gctccagttc tttgttgatc tggatggtcg tgaacacaaa
     1021 ccgcaactgg ccggcgaccg gttgctggcg caccaggaac tccaggcaca gccggttgag
     1081 ctcggtttcc acctcgtcaa tgtactgatc ccgcaagatt accgaatagg ccaactgccg
     1141 attccgctcc aacagcgcgc gcaggctcgc cttgagcgcc cgttcgccca agccggccat
     1201 ctcggtgact ttgcttcgga tgagatccat atcccgctga agggactctt caaaatgtga
     1261 atgcgtattc aatcgtgtag ccacgcaccc gttgtttgta tttcgtaatc ggttgtcaag
     1321 cgcaccgtca ctatgaaccc ggcgtgttac gggaaagcgt cggatttgtt acaattaggt
     1381 tacaacgcgc ccggagtctt tccctcaggt tccacacggc ggagcggctg ctttttgtcg
     1441 tgcgcttttg agcgaatcag gtagaactac gccgctatgt tttcacttca gaagctattg
     1501 ggcaaagaag acaagttctt caccctcctc gaagccagcg ccgaagaggc ccgcggcagc
     1561 gtgcaggcgc tcgtcaagct gagcaagacg ctcgataacc cgccgcctgc cgaggacttc
     1621 gcctatgccc ggcaaaaggg caagcagatc acgcgggaaa tcagcgccgc cgtctatacc
     1681 acctttatca ccgcgctgga acgcgaggac atcgaggaac tgtccaatgc gctttataag
     1741 atcccgaaga tgattgataa gttcgccacc cgcctcctgg caacgccgca atacgtgcga
     1801 gggatagatt tttccaggca gatcggcctg ctggagcggg ccacggacat cgtcctggaa
     1861 cttgtaaaat cactgcgcca aggcatggac ctggagaagg taaaggacct aaatgacaag
     1921 ctgcagttcc tcgaaagcga agccgacacc cacatgatgt cactctacaa ggatctgttc
     1981 agcggcaagc acgaaccggt ccaggtcatc gtgctaaagg acctctacga actgctggag
     2041 aaagtcatcg accgctgccg gaatgccggc aatgtcatcg ccaacatcgc gctgaagaat
     2101 tcctgaagcg caatgactct cctgctgacg gtcatcgtcg tagcgttggt ctttgagttc
     2161 atcaacggct tccacgacac ggccaactcg gtagccgccg tggtcgggac gagggtgctc
     2221 acccccacgc aggccatcgg tttggccgcg gtcaccaacc tgtgcggcgc gctggcgggg
     2281 accgcggtag cgaccacggt gggccaaggt ctcgtggacg cacattgcgt cacaggcgtg
     2341 acgctgatct gcggtctgct gggggcaatt ctctggaacc tgatcacatg gcggctgggg
     2401 ttgccctcga gctccagcca cgcgctgatc ggcggcctga tcggctccgc cgtcgcctcg
     2461 gcgggcaaca attggaatgt ggtgatttgg tcggcgccgg tggccggcaa gccttggtac
     2521 gcctggggcg gcgtgctcta caaggtcatc atccctatgt tcagctcccc catcctcggc
     2581 tttgttgtcg gattcctatg gatgacgctg ctctatatcc tgctgtcacg ttgccggcct
     2641 tcgagcatca acaaggcatt cagcaagctg cagattctca gcgccgcctt tttgggcttc
     2701 agccacggca gtaacgacgc gcaaaagacg atgggaatcg tcgccctggc cctcttctcc
     2761 gccaccacca tcggcgatct gactcacctt ccctcctggc tccagttcct gcacaccccc
     2821 gagttcaaaa ttgcgctttg ggtgaaactc gcctgcgcgt ttacgatggc tgccggcacc
     2881 gccactggtg gccgccggat catcaaaacc ctcggccgca aagtatcccg actccagccg
     2941 atcaacggct ttgcggcgga caccacttcg gccacggtgc ttatgacaac cgcagcgttt
     3001 ggcatgccgg tctccacaac ccacgccgtt tctacctcga tcatgggagt cggcacggcg
     3061 aggaaccaca aggccatgaa gtggcacgtg gtagaaagca tcatctggac gtgggtcatg
     3121 accttgcctg cgaccgggtt actcgcttat gcgttgaggc gtatttttct cgcactcggc
     3181 tcgggggcat gaacgaattg ccatgccaga attgtgttgc gcgccagacc acttgcgccc
     3241 cggtgcgtgc tgccggaaag ggcaacgccg ccaggtgcgg cacggcgtct gccagccact
     3301 aactaacgcc ctatgaccct cctattgacc gtcatattgg tggcgctggt cttcgaatac
     3361 atcaatggct ttcacgatac ggccaactcg atcgccaccg tggttgccac taaagtgctc
     3421 acgccgcgcc aggccgtcct gctggccgcc tgcaccaatc tgctcggcgc gctgtggggc
     3481 accgcagtgg cacgaaccat cgcctcgggg ctgatcgaca ccagactagt cctgctcacc
     3541 tcggacgtta tgatttgcgc cttgctgggc gcgatcatct ggaacctcat tacctggtgg
     3601 ttcggacttc cctccagctc gagtcatgcg ctgattgggg gcctgtgcgg ttcggcgctg
     3661 gccgcttcga acaacaactg gcaggttatc atttggtccc agccgaaccc acagcattgg
     3721 taccagggca aaggcatgct ctggaaagtg attgtgccga tggtcacctc gcccatggcc
     3781 gggctgatct tgggcttcat cgtcatggcg ctgctctatc tgctgctgca acgggctcgc
     3841 ccgaactggg tcaatcgttt cttcggccgg gcgcaaatgc tgagcgcggg ggcaatgggc
     3901 atcatgcacg gcacc
//
LOCUS       DATCGS010000267         3479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_21997, whole genome shotgun sequence.
ACCESSION   DATCGS010000267 DATCGS010000000
VERSION     DATCGS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21997"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VIL39_05505"
     CDS             <1..773
                     /locus_tag="VIL39_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5480916.1"
                     /translation="LKWFRNKKFRQAFSCAIDRDRLVREVYGGHAQPASGFISTENQK
                     WNNPDIPRYGFDLSRARTLLAEIGIRDRNGDGVMEDADGNPIEVLFYSNAGNPLREKA
                     TALIQEDLKKLGVRLVHTSLAYQALVDRINVTFDYECALMGLGGGGGDPASQVNVLRS
                     SEELHQWFPLQKAPSTGWEARLDTLMDAQMRTLDFAQRKKYFDEVQVILAEELPMIYT
                     VAPFAAAAIHPDIANLRPAVLSPYHLTWNLEELCFKKK"
     gene            complement(808..1695)
                     /locus_tag="VIL39_05510"
     CDS             complement(808..1695)
                     /locus_tag="VIL39_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5480917.1"
                     /translation="MRHLRYFVAVAEDLSFTKAAAKLHLAQPSITRQIHNLEEEIGVG
                     LLNRSRSQVALTEEGRSFLVDARRILALAAESILAVQRLSRGETGQLNIAYLSNSDVE
                     LLPATLGAFRQAFPHIALNLFDMAPAEQFRALEARKIDLGFVGLPPPATLGHLKWESI
                     TRHRTVVVLPIKHPLARKPQVSLRELKTMFFVGLSEKTHPGFRNWLSATCQQAGFTPR
                     ILQDAEMEPALMTFVAEGLGVSLAREHIKKLPHPGVAFRPLTPPIKSDYCIAWNRGND
                     SRALQQYIEIVKGLAARAH"
     gene            1887..>3479
                     /locus_tag="VIL39_05515"
     CDS             1887..>3479
                     /locus_tag="VIL39_05515"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEY5480918.1"
                     /translation="MRKCNCSGHCGPQPLNRREFIGLVGAGAAATLLGNTAWGAFELP
                     AEDLQRWRRELFAPAKPRLYHSDKHTDARMHLGGIGTGNFEIGVDGQLTTWQLFNTLR
                     DGQVPFHFLIRAGATTKLLQTAGGPDWPRVKQIEMTGEYPAAVLRFRDADLPVQVELT
                     AFSPFAPLDTRLSSMPLAAFVFQIKNPTNQKQTVSLTAVMQNPVGYEAAGENNGATNP
                     SFGGNVNEVLREGGAGGLFMRAEAGSEPTLDKPVTIWTAANLKGLNTPPPDRPKNLNV
                     QVLDAKPLRADKLTDPAHTVIWLEEAGSDIPASLLRAAQEAVQAGATLLFSGKSMPLL
                     EAYASWTGGKPVAEVNQRADILFEDFEHGYGKWKVEGEAFGKEPARGTLPNQNPVSGF
                     LGQGLVNSYLAGDDTRGRLISQPFTIERRFIRFLVGGGHYANTQIRLVLDGKVVRATS
                     GTDSEQLQPATWDVGAFQGQTAHIEIVDEQQGGWGHINADQIEFSDLPGNRALMQLLE
                     ELLPARFSGIRPAGGLASGTKAV"
BASE COUNT          689 a         1074 c         1099 g          617 t
ORIGIN      
        1 ggctgaagtg gttccggaac aagaaattcc gccaggcctt ctcgtgcgct attgaccggg
       61 accgactggt gcgcgaggtt tacggtggcc acgcacagcc cgcctcgggc ttcatctcga
      121 cggagaacca gaagtggaac aacccggata tcccgcgtta tggcttcgac ctctcacgcg
      181 ctcgcaccct gctcgcggag atcggcatcc gggatcgcaa tggcgatggc gtgatggagg
      241 acgccgacgg gaacccgatt gaggtcctgt tctattccaa tgccggcaat ccccttcgcg
      301 aaaaggcgac cgccctgatt caggaggacc ttaagaagct gggggtcaga ctcgttcaca
      361 cgtccctcgc ctaccaggca ctcgttgaca ggatcaatgt caccttcgac tacgagtgcg
      421 ctctgatggg cctgggtggc ggcggcggcg accccgcctc tcaagtgaac gtgctccggt
      481 ccagcgaaga actccaccaa tggttccccc tccaaaaagc tccttcgacc ggctgggagg
      541 cccgcctcga caccctcatg gatgcccaaa tgcggacgct ggatttcgcg cagcgcaaga
      601 aatacttcga cgaagtgcag gtcatactag ccgaggagct accgatgatt tacaccgttg
      661 cgccgttcgc cgccgctgcc atccatcccg acattgccaa cctccgtccc gctgtcctga
      721 gcccttacca cctcacttgg aacctcgagg aactctgctt caagaagaag tgacgctcat
      781 tagaataaaa agggcggccc tggcggctca gtgcgcgcgc gcagcgaggc ccttgacgat
      841 ctcgatatat tgttgcaagg ctcgtgaatc gttaccccgg ttccaggcaa tgcagtagtc
      901 gctcttgatc ggcggcgtca agggccggaa ggccactccg ggatggggca atttctttat
      961 gtgctcgcgc gccagcgaga cgcccaaacc ttcggcaacg aaggtcatga gggcgggttc
     1021 catctcggcg tcttgcagaa ttctcggagt aaagcccgcc tgctggcacg ttgcgctgag
     1081 ccaattgcga aaacccggat gcgtcttttc ggacaggccg acaaagaaca tcgttttcag
     1141 ctcgcgcagg ctcacttggg gtttccgggc cagcggatgt tttatcggca gcaccacgac
     1201 cgttctatgg cgggtgatgc tttcccattt caggtgcccc agggttgccg gaggaggcag
     1261 ccccacaaag cccaaatcaa tcttgcgcgc ctcgagggcg cgaaactgtt ctgcgggagc
     1321 catgtcaaag aggttgagcg caatgtgggg gaacgcctga cggaaggccc cgagcgttgc
     1381 ggggagcagt tcgacgtcag aattggacaa atacgcgatg ttcaactggc cggtttcgcc
     1441 ccggctgagg cgctgcaccg ccaggatgct ttccgccgcc agggccaaaa tgcgccgggc
     1501 gtcaaccagg aaagaccgcc cttcctcggt taaggccacc tggcttctgg aacggttcag
     1561 caggcccact ccgatttcct cctccaggtt gtggatctgg cgcgtgatcg agggttgggc
     1621 taaatgcagc tttgcggcgg ctttggtgaa gctcaaatcc tccgccaccg ccacgaaata
     1681 tcgcagatgc cgcaattcca tagactagca cgaaacttag cagctttggc cgcgttagca
     1741 accgctgaga gcacgctcag ggcatcgccc agcggtagcg taccttttcg cccagccgct
     1801 ttcctgcttg gcagcgcgat cgcagcctga tagcagagtc accaatgaag atcagcacgg
     1861 ataactggaa ctgaaggagg caaatcatgc gcaaatgcaa ctgttcggga cattgcgggc
     1921 cccaaccact taaccgccgt gagttcatcg ggctagtggg cgcgggagcg gcggcgacgc
     1981 tgctgggcaa tacggcgtgg ggggcattcg agctcccggc tgaggacttg cagcggtggc
     2041 ggcgtgagct gttcgccccg gccaaaccgc ggctctatca ctcggacaag cacacggacg
     2101 cccggatgca cttgggcggc atcggcacgg gcaatttcga gatcggcgta gatggccaac
     2161 tgaccacctg gcagcttttc aacacgctgc gggacggtca ggtgccgttt cattttctga
     2221 tccgggccgg cgccaccacg aagctgctgc aaactgcggg agggccggac tggccgcgcg
     2281 tcaagcagat cgagatgacc ggcgaatacc cggcagccgt gctgcggttc cgggacgcgg
     2341 acctgccggt ccaggttgag ctgacggcgt tcagcccctt tgcgccgctc gacacgcggt
     2401 tgtcatccat gccgctggcc gcgttcgtct tccagatcaa gaacccgacg aaccagaagc
     2461 aaacggtatc cctgaccgcc gtgatgcaga acccggtggg ctatgaggcc gccggggaga
     2521 acaacggggc aacgaatccc agctttgggg gcaacgtgaa cgaggtattg cgggaagggg
     2581 gggccggcgg attgttcatg cgggctgagg ccggcagcga gccaacgctg gacaagcccg
     2641 tgaccatctg gacggcagcc aacctgaagg gtctcaacac gccgccgccc gaccgaccga
     2701 agaacctcaa cgtccaggtg ctcgatgcca agccgctgcg cgccgacaaa ctgaccgatc
     2761 ccgcccacac cgtcatctgg ctggaggaag ctgggtcgga catccccgcg tcgttgctgc
     2821 gcgccgcgca ggaagcggtg caggcggggg cgacgcttct cttctccggc aaatccatgc
     2881 cgctgctgga ggcgtacgcg tcgtggaccg gtggcaaacc ggtcgcggag gtcaaccagc
     2941 gggcggacat cctgttcgag gacttcgagc acggctacgg caagtggaaa gtcgagggcg
     3001 aagcgtttgg caaagagccg gcccgcggca cgttgccgaa ccagaacccg gtatccggct
     3061 ttctcggcca gggcctcgtc aacagctatt tggccgggga tgacacgaga ggtcggctca
     3121 tcagccagcc gttcaccatc gagcgccggt tcatccggtt cctggtgggt ggcgggcatt
     3181 acgccaacac gcaaatccga ctggtgctgg acggcaaggt cgtgcgcgcg acctcgggca
     3241 cggacagcga gcagctccag cccgcgacgt gggatgtcgg ggccttccag ggccagaccg
     3301 cgcacatcga gattgtagat gagcagcaag gcggctgggg ccatatcaat gcagaccaga
     3361 tcgagttctc ggacctgccc ggcaaccggg cactgatgca gttgctcgag gagttgcttc
     3421 cggcgcgctt cagtggtatt cgtccggcag gcggcctggc cagtggcacg aaagcggtc
//
LOCUS       DATCGS010000268         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_270984, whole genome shotgun sequence.
ACCESSION   DATCGS010000268 DATCGS010000000
VERSION     DATCGS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270984"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1126
                     /gene="tatC"
                     /locus_tag="VIL39_05520"
     CDS             95..1126
                     /gene="tatC"
                     /locus_tag="VIL39_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5480919.1"
                     /translation="MAEKPEADRLDAQEEEEGGPVKSFLEHLEDLRWVLIKSVAALGV
                     AILICLIGGNYVVLILKHPLTKTKVPYSGTNQVVTVVFGTNRLGVFSLTAEQQGGLDL
                     GTNRFVAVRVEPAVIGTNRVLAWRLDDNLDLAERAQGLSVDLINLSPASGFFVAFQVA
                     LYGGIVLASPFIFYFVASFVFPALKMKERKYVYGGLAYGLTLFLVGVAFCYFVLMPVA
                     LAASQRYSQWLGFSAFQWKADEYISFVCKFMLGMGLGFEMPVIILTLVKIGVLNYSIL
                     SKARKYMIVINLVLGAVLTTPEVITQLIMFLPLQTLFEITIWITWYWERRDKKREAAQ
                     GAGDIVAGT"
     gene            1279..1722
                     /locus_tag="VIL39_05525"
     CDS             1279..1722
                     /locus_tag="VIL39_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HEY5480920.1"
                     /translation="MRNIFAFLATLVIAGILSSGCSSAEKKLGRGVSNMTEFARLGEI
                     RRSVEQTAIFDQPGGHYTTGFIRGFNKTLARTGVGVYEVVTAPFPPYGPVCTSYLSPN
                     PVYPDNYSPDILDDALFATDTNMGFSGGDVAPFIPGSRFKIFNTQ"
     gene            1867..2739
                     /gene="ispE"
                     /locus_tag="VIL39_05530"
     CDS             1867..2739
                     /gene="ispE"
                     /locus_tag="VIL39_05530"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY5480921.1"
                     /translation="MLEKLSPCKVNLLLNTLGRRPDGFHELETVLQPLRFFDRLSFVR
                     VPQGIQLSCSVPGLPTDARNLVYRAAALFLEAAGIKEGVRVELQKNIPLAAGLGGGSG
                     NAATTLLGLNELFGGPLAPGELQRLAASIGSDVPFFLQSQPALATGRGERIEPLESFP
                     ALKGAAFVLIHPGFGIATPWAYQHLARFPHAVNGRPGRAQELISRLRSADHLATAGAE
                     FYNSLEAPALEKYPLLALFQEFLRANGAAAALMSGSGSTTFGVFPSQAAAEALVEKLK
                     SRFGEANWMVVVRA"
     gene            complement(2789..>3299)
                     /locus_tag="VIL39_05535"
     CDS             complement(2789..>3299)
                     /locus_tag="VIL39_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480922.1"
                     /translation="QAIARESNSAPAHYYFGMNLGQLARTKGLTALRLVSQMQREFAR
                     ARDLDERLDWAGPDRNLGLLYADAPTIGSIGSRARAREHLKRAADLAPQYPENRLNLI
                     EAYLNWGESNNAQRELAALQRLWSSAHTNFVGEAWAAGWADWEPRLKKLQKKIEAPPK
                     SLGAPRTQP"
BASE COUNT          593 a         1015 c          983 g          708 t
ORIGIN      
        1 ccaacaccac ccctacacag cccgaggcgc cgccgccgac tacttccccg tccactcccg
       61 gcaccaaggc ctaattcgct gagcggtttc agctatggcc gaaaaacccg aggcggaccg
      121 cttggacgca caagaggaag aagagggcgg gcccgtaaag tcgtttctcg agcacctcga
      181 agacctgcgt tgggtgctca tcaagagcgt ggccgcgctg ggcgtggcca tcctcatttg
      241 tctgattggc ggcaattacg tcgtcctcat tctcaaacac ccgctcacaa agacaaaagt
      301 tccctattcc ggcaccaacc aggtcgttac cgttgtcttc ggcactaacc ggctgggggt
      361 gttttcgctc accgctgagc aacagggggg cctggacttg ggcaccaacc gctttgtcgc
      421 cgtccgggtc gaacccgccg tcattggtac caaccgggtg ctggcgtggc ggctggatga
      481 caatctcgat ctggcagagc gcgcacaagg gctaagcgtt gacctgatta acctcagtcc
      541 ggccagcggt tttttcgttg ccttccaggt cgccctttat ggcggcatcg tcctggcctc
      601 gcctttcatt ttctacttcg ttgcctcgtt tgtatttccc gccctcaaga tgaaggagag
      661 gaaatacgtc tatggcgggc tggcttacgg tctcacgctg tttttggtcg gcgtggcctt
      721 ctgctatttc gtgctgatgc cggtggcgct cgccgcatcg caaaggtatt cccaatggct
      781 cggcttctcc gccttccaat ggaaggcgga cgaatacatc agcttcgtct gcaaattcat
      841 gctcggcatg ggattgggat ttgaaatgcc agtgatcatt ctcaccctgg tgaagatcgg
      901 cgttctcaat tactcgatcc tttccaaggc ccgcaagtac atgatcgtca tcaacctcgt
      961 cctgggcgcc gttctaacca ccccggaggt catcacccaa ctcatcatgt tcctgccgct
     1021 gcaaacgctt ttcgagatca ccatctggat cacctggtac tgggagcgac gggacaagaa
     1081 gcgcgaggcg gcacagggcg ctggggatat cgtagcgggc acttgaggcg tgatgcgtga
     1141 tggatcagaa tcgaccaacc cctgtttcgc cggtcacgca tcacgcatca cgcctcacgt
     1201 ataaaccttc accttttttc aattcgccct ttacaagccc ccaggtttgg ctaaattaca
     1261 tgccagtaat gaacatttat gcgtaacata tttgccttcc ttgccacctt ggtaatcgcc
     1321 gggatcctga gctctggatg ctccagcgcg gaaaagaaac tcgggcgcgg cgtgagcaac
     1381 atgacggaat ttgcccgact cggagaaata cgccgctcgg ttgagcagac cgccatattc
     1441 gatcaacccg gcggtcatta caccaccggc ttcattaggg gattcaacaa gaccctcgcg
     1501 cgcacgggcg tcggcgttta tgaagtcgtg accgctccct tcccgcctta cggtccggtg
     1561 tgcacctctt acctctcacc caaccccgta tatcctgaca actatagccc ggacatcctt
     1621 gacgacgcgc tgttcgcgac ggacactaac atgggattca gcggcggtga cgtcgcgccg
     1681 ttcattccgg ggagccgttt caagatcttc aatacgcagt aatatccttt tgggctgctg
     1741 atcggttttg cgccagcatt tgcgtgctgg cgcttttttt gcagcggaag cctggcgtcc
     1801 gcctgcaaga gtgcggccaa cttttagtgt ccttccccgc ccttttgtgg caaatcaatc
     1861 accgtgatgc tggaaaaact atcgccctgc aaagtcaacc tgttgctgaa cactctcggc
     1921 cggcgcccgg atggctttca cgagctggag acggtgctgc agccgctccg gttcttcgac
     1981 cgacttagct tcgtgcgggt gccccagggg atacaattaa gctgcagtgt gcccggcctg
     2041 ccgaccgacg cgcggaactt ggtgtatcgg gctgcggccc tgtttctgga ggcggcagga
     2101 atcaaggagg gggtacgcgt ggaactccag aagaatattc ccctggcggc aggtctgggg
     2161 ggcggcagcg gcaatgcggc gactacgttg ctcgggctga acgaactatt cggcgggccg
     2221 cttgcaccgg gagagttgca gcgcctcgcc gcgtccatcg gctccgatgt gccgtttttc
     2281 ctgcaaagcc agccggcgtt agcgacgggc cgcggcgagc ggattgagcc gctggaatcg
     2341 ttccccgccc tgaaaggcgc ggcgtttgtg ctgattcatc cgggcttcgg catcgccacc
     2401 ccctgggctt accagcacct agcgcgcttc ccgcacgcgg tcaacggccg ccccgggcgg
     2461 gctcaggagc tcatctcgcg gctgcgaagc gccgatcatc tggcgacggc gggggcggag
     2521 ttctacaact cgctcgaagc gccggcactc gagaagtacc cgttgctggc gttgttccag
     2581 gaattcctgc gcgccaacgg cgcggcggcc gcgctgatgt ccggtagcgg ttcgaccacc
     2641 tttggcgtat ttccgagcca ggcggctgcg gaagcgctgg tcgaaaagct caagtccagg
     2701 tttggcgagg cgaattggat ggtcgtggtg cgcgcctgac agcaggcgaa ggcccgaggc
     2761 gcaagggccg aatgaatgtg gctggaggct acggctgagt ccgcggcgct ccgagcgatt
     2821 tagggggggc ctcgatcttc ttctggagct tcttgagccg cggttcccaa tccgcccagc
     2881 ctgccgccca ggcctcgccg acgaagttgg tgtgcgcgct agaccagagt ctttggagag
     2941 cggccaactc acgttgggcg ttattggatt cgccccagtt cagataggcc tcgatgaggt
     3001 tcagccggtt ctcgggatat tggggagcca gatccgcggc gcgcttcaaa tgctcccgcg
     3061 cccgggcgcg gctcccgatg ctgccgatgg tcggagcgtc ggcgtaaagc aaccccaggt
     3121 tgcgatcggg accggcccag tcaagccgct cgtccaggtc gcgtgcccgc gcgaactcgc
     3181 gctgcatctg gcttaccagt ctgagcgcag ttagcccttt ggttctcgcg agttgcccga
     3241 gattcatgcc gaagtagtag tgggcgggcg ccgagttgga ctcgcgcgca atcgcctgc
//
LOCUS       DATCGS010000269         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_73488, whole genome shotgun sequence.
ACCESSION   DATCGS010000269 DATCGS010000000
VERSION     DATCGS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73488"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VIL39_05540"
     CDS             <1..594
                     /locus_tag="VIL39_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5480923.1"
                     /translation="AFFLYRQNQAEITASEALSSVAMPQASGARADTVEGYLKVAATH
                     PRSKAGARALLLAAGGLFVDGKYDEARTQFERFRREYSDSLFMGEALLGIAACLDAQG
                     KTRDAMAAYKDLIDHRPGDSNVPQAKFALARLHQAQNEPEQARNLFEDVERSQPYGSL
                     GDEARMQLEELKTKFPKLLAPVTTMPTNAAPLKVEKR"
     gene            594..1883
                     /locus_tag="VIL39_05545"
     CDS             594..1883
                     /locus_tag="VIL39_05545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY5480924.1"
                     /translation="MKLTLIGTGYVGLVTGACFAEVGHQVVCVDNDAAKVKVLQEGGI
                     PIYEPGLEELVKRNVAAGRLSFTTSTIEGVEKSDVIFIAVPTPPLPDGSVDMSFLEKV
                     AREIAGAMTSYKIVVDKSTVPVQTGDKVSETIKRYCKARVDFDVVSNPEFLREGFAVE
                     DLMQPDRIVVGVRSQRPVAALEEIYAPFKAPIIVTDINSAELIKHASNSFLALKISYI
                     NAIAVLCEATGANVQEVANGMGMDERIGRRFLQASLGFGGSCFPKDLSAFIKIAEQVG
                     YDFRLLKEVQQINVAQMERFVKKILETLWVLKGKTIGVLGLAFKQNTDDVRMSPAIEL
                     CQRLQKEGAILRVHDPKAMEKAKAILKDVTFVEDMNAVAEGCDALIIATEWNEFKKLD
                     LERVRKSLTHPIMFDGRNLFDPAEMERLGFIYKSIGR"
     gene            2046..2450
                     /gene="mutT"
                     /locus_tag="VIL39_05550"
     CDS             2046..2450
                     /gene="mutT"
                     /locus_tag="VIL39_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY5480925.1"
                     /translation="MPIIDVSAALVFREGKLLITQRYADAHLGDLWEFPGGKREPNET
                     FEACLQRELREELGIEVAVGELVESLTHAYPEKTVYLKFYRCRWEEHEPQTLGCSAFK
                     WVTADELKDYAFPAADAQLLDKLKDDTELWGL"
     gene            complement(2570..>3310)
                     /locus_tag="VIL39_05555"
     CDS             complement(2570..>3310)
                     /locus_tag="VIL39_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5480926.1"
                     /translation="AAPTADEAETKLKLWAQYSDTDGGILKDVITYQPGERGQRTLVL
                     IKPENFRFPTGRPGNMIDFFSRTGLFIVAVKVHRMSVAQALEFYGPVRETLRTKLKDV
                     VAAKAKAAIEKEMGFKVGREQEQQLGAILGPLFGDNQFENIVRFMSGRAPSECPPGEE
                     NTPGSEKCIALVYEGVGAVGKIRDVLGPTDPSKAPPGSIRREFGQTIMVNAAHASDSE
                     ENSKREIGIVNVGENTFRQIVEKFYGKA"
BASE COUNT          677 a          948 c         1009 g          676 t
ORIGIN      
        1 gccttctttc tctataggca aaaccaagca gagatcaccg ccagcgaagc attatccagt
       61 gttgccatgc cgcaagcgtc cggcgctcgc gctgataccg tcgaagggta tttgaaggtc
      121 gccgccacgc atccgaggtc caaggccggc gcgcgcgcgt tgctgcttgc ggccggcgga
      181 ttgttcgtgg acggcaagta tgacgaagcc aggactcagt tcgagaggtt ccggcgcgaa
      241 tactcggaca gccttttcat gggggaggcg ctgctgggga ttgcggcctg tctggatgcg
      301 cagggcaaga cgcgcgacgc gatggccgcc tacaaagatt tgatcgatca ccgtccgggc
      361 gatagcaatg tgcctcaggc caagttcgcc ttggcgcgcc tgcaccaagc gcagaacgag
      421 ccggaacagg cgcgcaacct tttcgaggac gtggaacggt ctcagcctta cggttcgctc
      481 ggggacgagg cgcggatgca gcttgaggaa ttgaagacaa agtttccgaa gctcctggcg
      541 cctgtgacca ccatgcccac caacgctgcg ccgctcaaag tcgaaaagcg ctgatgaagc
      601 tcaccctcat cggcacaggc tacgtgggcc tggtcacggg ggcctgcttt gccgaggtcg
      661 gtcatcaggt ggtctgcgtc gataacgatg cagccaaggt gaaggtgctc caggaaggcg
      721 gcattcccat ctacgagccg ggtctggaag agttggtgaa gcgcaacgtc gccgctggcc
      781 gcctcagctt caccaccagc accatagagg gggtggaaaa atcagacgtc attttcatag
      841 ctgtccccac gccgcccttg ccggatgggt cggtggacat gagcttcctc gaaaaggtcg
      901 cccgcgaaat cgccggcgca atgaccagct acaaaatcgt cgttgataag agcaccgtgc
      961 cggtccagac tggcgacaag gtctccgaga ccatcaagcg ctactgcaaa gcccgcgtcg
     1021 atttcgacgt ggtcagcaac cccgagttcc tccgcgaggg gtttgcggtc gaggatttga
     1081 tgcagccgga tcgcattgtg gtcggcgtcc ggtcgcagcg gcccgtggcc gccctcgagg
     1141 agatctatgc gccgttcaag gcccccatta ttgtcacgga cattaattcg gcggagctga
     1201 tcaagcacgc gtccaattcc tttctcgcgc tcaagatttc ctacatcaac gccatcgccg
     1261 tattgtgcga ggccacgggc gccaacgtcc aggaagtggc caacggcatg ggcatggacg
     1321 aacgcattgg ccggcgattc ctgcaggcct cgctcggttt cggcggaagc tgctttccca
     1381 aggacctcag cgccttcatc aagatcgccg aacaggtggg ctatgatttc cggttgttga
     1441 aggaagtgca gcaaattaac gtggcgcaga tggaacggtt cgttaagaag attctggaga
     1501 cactctgggt gctcaagggt aagaccattg gcgtgttggg cctggcattc aaacagaaca
     1561 cggatgacgt ccgcatgtct cccgcgatcg agctttgtca gcgcctgcaa aaagaaggcg
     1621 ccatcctgcg cgtccacgat cccaaagcca tggagaaagc caaagctatc ctcaaggacg
     1681 ttacgttcgt cgaagacatg aacgcggtcg ccgagggttg cgacgcgctg atcattgcca
     1741 cagagtggaa cgaattcaag aagctcgatc tcgaacgcgt ccgcaaatcc ctgacgcacc
     1801 cgattatgtt cgacggccgc aatctctttg acccggccga aatggaacgt ttgggattca
     1861 tctacaagag cattggccgc tgacggccct gggcggaatg ggcggtggag aggttaaatg
     1921 agttaaatag gttacatggg ttacatgggt tacatcggtt gcagcgccgt tctctccccg
     1981 attggccgta ttttagctat gtaacctatg taacttattt aacccattta actgaattaa
     2041 cccctatgcc catcattgac gtttccgctg ccctggtctt ccgcgagggt aaactgctta
     2101 tcacccagcg ctacgccgac gcgcatctgg gcgatctgtg ggagtttccc ggcggcaaac
     2161 gcgagccgaa cgagactttt gaggcctgtc tgcagcggga attgcgcgag gagttgggca
     2221 tcgaggtggc tgttggtgag ttggtcgaga gtctgactca cgcttacccg gagaagaccg
     2281 tttacctgaa gttctatcgc tgccgatggg aagagcatga accgcaaacg ctgggctgtt
     2341 ctgccttcaa atgggtgacg gctgacgagt tgaaggatta cgcctttccc gccgccgacg
     2401 cccagttgct ggacaaactg aaggacgaca ccgagctttg gggtctgtga gatcctgtaa
     2461 gccgcctgtt tccaggcggc tcagaggacg aagttagacg tcaggccagc gaagggtacg
     2521 gccctgaacg gtctcgggcg ggggtaacga agcggggacg cccgccccac tatgccttcc
     2581 cgtaaaactt ctcgacgatc tggcggaagg tgttctcgcc gacattcacg atgcctattt
     2641 cgcgcttgga attctcctcc gaatcgctgg cgtgcgcggc attgaccatg atagtctggc
     2701 cgaattcgcg gcggattgat ccgggcgggg ccttagacgg gtcggtcggg ccgagcacat
     2761 cgcggatttt gcccacggcc ccaacacctt cgtaaaccag cgcgatgcac ttttccgagc
     2821 cgggtgtatt ctcctcgccc ggaggacatt ccgagggagc gcggccggac atgaatcgga
     2881 cgatattctc gaattggttg tcgccaaaaa gcgggccgag gatggcgccg agctgttgct
     2941 cctgttcgcg tccgaccttg aagcccatct ccttttcgat ggcagctttg gctttcgcgg
     3001 ccacgacatc cttaagcttg gtgcgcaggg tctcgcggac cgggccgtag aactccagcg
     3061 cctgggccac gctcatgcga tgcaccttaa cggcgacgat gaacaggccg gtgcgggaga
     3121 agaaatcaat catattgccg ggccggccgg tgggaaagcg gaagttctcc ggcttgatca
     3181 gcaccagcgt acgttgcccc cgctcgcccg gttgataggt gatgacatct ttcaagatcc
     3241 cgccatcggt gtccgagtat tgggcccaga gcttgagctt cgtttcggcc tcatccgccg
     3301 tcggcgcggc
//
LOCUS       DATCGS010000270         3621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_369572, whole genome shotgun sequence.
ACCESSION   DATCGS010000270 DATCGS010000000
VERSION     DATCGS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369572"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VIL39_05560"
     CDS             complement(<1..1401)
                     /locus_tag="VIL39_05560"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEY5480927.1"
                     /translation="MLKSSARPAIRLRGVRHNNLKNFDLDLPLNQFVVITGLSGSGKS
                     SLAFDTLFAEGQRRYIETFSPYARQFFDRMDKPQVDSIEGIPPAIAIEQRNAVKTTRS
                     TVGTMTEICDYMKMLWPHVAQLHCRQCGQPVRKDSPQQIWHALQAGEQQQPAADEVLI
                     TFELPLSEKLSLEESLALVAKQGYQRLLLDDQILQLEDVPSRITDHASRITSLTIIQD
                     RLRLTEASRARFVEACEQAYHFGKGKLAIHSLVTGGSDSKLKTRRPQLFSNRRHCAEC
                     DIAYREPSPALFSFNHPVGACPTCRGFGRTISIDYDLVLPDRSRTLAQGVVRPWQTGL
                     GAECQDDLLRSCGKFHVPTDIPFNRLPRKWQDFVIEGEPGYGKDEAHEWPRAWYGVKG
                     YFRWLESKAYKMHVRVLLSRYRAYTPCPDCHGKRFQPEALLYRCEAGSPKSKVQSPKQ
                     GRNPKPEVRTGLEPASS"
     gene            1580..1810
                     /locus_tag="VIL39_05565"
     CDS             1580..1810
                     /locus_tag="VIL39_05565"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEY5480928.1"
                     /translation="MKSLEDIESEALLLPRDQRFTLAHRILSSIEPDMKAEAEAVWDM
                     EIRERISKYDAGLTSGVPGQEVFAELDKKLEG"
     gene            1807..1953
                     /locus_tag="VIL39_05570"
     CDS             1807..1953
                     /locus_tag="VIL39_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480929.1"
                     /translation="MNYEFLTEARDEFWEAALYYESKEAGLGVRLRDEISHVIERILT
                     DPYL"
     gene            2303..>3621
                     /locus_tag="VIL39_05575"
     CDS             2303..>3621
                     /locus_tag="VIL39_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY5480930.1"
                     /translation="MASEARSATGSGTGFQSHVPHGATMRECTPRAIILGTLLGVVFG
                     ASSLYLVLKVGLTVSASIPVAVISLALFRLWSKAGGRDATILENNIVQTAGSAGESIA
                     FGLGVTMPAIMILGFDLEISRVMLVGLLGGLLGILMMIPLRRALIVAQHGQLKYPEGT
                     ACAQVLKAGAAGASPAAAKGAKPAAPLADTGIKASTIFTGFGIGLAYKTLMAAFRLWK
                     DIPEKVFGAPLKGGSISAEISPELLGVGYIIGPRIGSIMMAGGVLSCLVLTPLIKFFG
                     EGLSGVLAPGTKRIADMSPGDIRTAYILYIGAGAVAAGGIISLMRSLPIILHSVRSGL
                     SDFKTAAVARTSVLRTDRDLSMKFVGVGILVLILAILAAPSLHMNILGAVMIVVFGFL
                     FVTVSSRLTGEIGSSSNPISGMTVATLLLTCLVFLIVGWTGGTYYVTA"
BASE COUNT          626 a         1039 c         1187 g          769 t
ORIGIN      
        1 ggaggaggca ggctctagcc cagttcggac ttcgggcttc gggtttcggc cttgtttggg
       61 actttggact ttggactttg gactcccagc ctcacatcgg tacagcagtg cttccggctg
      121 gaagcgtttg ccgtggcaat ccgggcaggg cgtgtaggca cggtatcggg agagcaggac
      181 gcgcacgtgc atcttgtagg ctttcgattc cagccagcgg aagtagcctt tgacgccata
      241 ccaggcgcgc ggccattcgt gcgcctcgtc cttgccgtaa cccggctcgc cttcgatgac
      301 aaagtcctgc cacttcctgg gcaaccggtt gaacgggatg tcggtgggca cgtggaactt
      361 cccgcaactg cgcagtaaat cgtcctggca ttcggcgccc aggccagtct gccagggtct
      421 caccacgcct tgcgccagcg tccgggagcg gtcgggcagc accaggtcgt aatcgatact
      481 gatggtgcgc ccgaagccgc ggcaggtggg gcaggcgccg acggggtggt tgaagctgaa
      541 cagggcaggg gatggctcgc ggtatgcgat gtcgcactcc gcgcaatggc gccgattgga
      601 gaagagctga ggccttctgg tcttgagctt cgagtcgctg ccgccggtaa cgaggctatg
      661 gatcgcgagc ttgcccttgc cgaagtggta ggcttgttcg caggcttcga cgaagcgcgc
      721 gcggctggcc tcggtgaggc ggaggcggtc ctggatgatg gtcagggacg tgatacgtga
      781 tgcgtgatcc gtgatgcgtg aaggaacgtc ctcaagctga aggatttggt catccagcag
      841 caggcgctgg tagccttgtt tggccaccag ggcgagggat tcttcaagtg agagcttctc
      901 cgacagcggc aactcgaagg tgatcaacac ctcgtccgcg gcgggttgtt gctgctctcc
      961 cgcctggagg gcatgccaga tttgctgcgg cgagtctttg cgcacgggct ggccgcactg
     1021 gcggcagtgc agttgcgcga cgtgcggcca gagcatcttc atgtaatcgc aaatctcggt
     1081 catcgtgccg actgtggaac gggtagtctt gacggcattg cgctgctcga tggcgatggc
     1141 gggcgggatg ccttcgatgc tgtccacctg gggcttgtcc atgcggtcga agaactgccg
     1201 tgcgtagggc gagaaggtct cgatatagcg ccgctggcct tccgcgaaca gggtgtcgaa
     1261 cgccagggaa ctcttgcccg agccgctcaa gcccgtaatg accacaaact ggttgagcgg
     1321 caggtcgaga tcgaagttct tcagattgtt gtggcggacg ccccggagtc ggatggcagg
     1381 ccgcgcagac gatttaagca ttgcaggacc aaggtagggt tgcgcaagcg gggttgcaag
     1441 ttggagggcg cgggtcgcga cttgccgcac ggagtctcct gttggagaag tgctggcatc
     1501 gtagcaatcg gcgtatgaat tcggctatga gacggcagtt cggcttgcca actctgccac
     1561 cggacggtaa cctctaacta tgaagtcgct cgaagacatc gagtccgagg ccctgcttct
     1621 ccctagggac cagcgcttca cattggcgca tcgcattctt tcgagcattg agcccgatat
     1681 gaaggccgag gcggaggctg tgtgggacat ggaaatccgc gagagaatca gcaagtatga
     1741 cgctgggctg acatcgggcg tcccgggcca ggaggtcttt gcggaactgg acaagaaact
     1801 ggagggatga attacgagtt tctcacagaa gctcgcgatg agttttggga agcggcgctg
     1861 tattatgagt ccaaggaggc gggtttgggc gtgcggctga gggatgagat ttcgcatgtt
     1921 attgagcgta ttctcactga tccatatctt tgacgggaac gcgctggggg atatcgacga
     1981 gtaaactgcc cggtctttcc gtattacgtc gcctatttca tccgcgccga gaaggtggtg
     2041 attgcggctg tagcacatgg gcaccgaaga cccgactatt ggaaggaccg agtgaaaggc
     2101 gtctgatccg cactgccttc ctgtggggat ggtccgctgt tcagcaaacc cacccctaat
     2161 ctgatggtat ggccgaagca tccctagcct caaacgcctt ggctgtcatc ggcaggcagc
     2221 agcgtgattc ggtggcgcgc gtcaccgctc cggccacttt tcgctcgcat tgggcggatt
     2281 ttagcagaag attccgcaac ttatggcgag cgaagcccga tctgccaccg gctccggcac
     2341 cggattccag tcccacgttc cgcatggcgc aaccatgcgc gaatgcacgc cgcgcgccat
     2401 catcctcggc accctcctgg gcgtggtgtt cggcgcttcg tcgctgtacc tcgttctcaa
     2461 ggtcggcctg acggtaagcg cgtcaatccc ggtcgcggtg atttcgctgg cgctgttccg
     2521 gctttggtcg aaggccggtg gacgcgacgc cactatcctc gaaaacaaca tcgtccagac
     2581 tgccgggtcc gccggtgagt ccatcgcgtt cggcctgggg gtcaccatgc cggcgattat
     2641 gatcctcggg tttgacctcg agattagccg ggtgatgctg gtgggtctgc tcggcggcct
     2701 gttgggcatc ctgatgatga ttccgctgcg gcgggcgcta attgttgctc agcacggcca
     2761 actcaagtat cccgaaggca ccgcctgcgc ccaggtgctc aaggccgggg ctgcgggggc
     2821 atcgcctgca gccgccaaag gagccaagcc cgcggcgccg ctggctgata ccgggataaa
     2881 ggcctcgacc atcttcaccg ggttcgggat tggcctggcc tacaagacac tcatggcggc
     2941 cttccggctg tggaaagaca ttccggagaa agtgttcggc gccccgctta agggtggctc
     3001 aatctccgca gaaatctccc ccgaactgct gggggtgggt tatatcatcg gtccgcggat
     3061 tggctcgatc atgatggccg ggggcgtgct tagctgcctg gtgctgacgc cactgatcaa
     3121 gttcttcggc gagggcctga gcggcgtgct ggcgccgggc accaagcgca ttgccgatat
     3181 gagccccggc gacatccgca ccgcctacat cctctacatt ggcgcagggg cggtcgccgc
     3241 cggcggtatc atcagcctaa tgcggtcgtt gcctatcatc ctgcacagtg tccggtcggg
     3301 actgtcggac tttaagactg ccgccgttgc ccgcacctcg gtcctccgca ctgaccgcga
     3361 cctgtctatg aagttcgtag gggtgggcat tttggtgcta atccttgcca ttctggccgc
     3421 gccgtcgctg cacatgaaca ttctgggagc ggtgatgatt gtggtgtttg gcttcctgtt
     3481 cgtcaccgtg tcctcccggc tcaccggcga gatcggttcc tcttccaacc ccatctccgg
     3541 gatgaccgtc gccacgctgc tgctcacgtg cctggttttc ctcattgtcg gctggaccgg
     3601 cgggacctac tacgtcacgg c
//
LOCUS       DATCGS010000271         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_369586, whole genome shotgun sequence.
ACCESSION   DATCGS010000271 DATCGS010000000
VERSION     DATCGS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369586"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VIL39_05580"
     CDS             complement(<1..213)
                     /locus_tag="VIL39_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY5480931.1"
                     /translation="MALDYSCLRTLTAREVIAALLRDGFSLDRQSGAHRLYRHPDGRR
                     VTVSFHTSGETFGIRTLRSMLEWQARW"
     gene            complement(217..459)
                     /locus_tag="VIL39_05585"
     CDS             complement(217..459)
                     /locus_tag="VIL39_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY5480932.1"
                     /translation="MTYNFKVIVEPDGDRWHAYCPALVSAGAATWGHTRTEALRNIQD
                     VVRMVVDSLVEHGEALPSTPSDQVQVLAEPQVAVIV"
     gene            801..2249
                     /locus_tag="VIL39_05590"
     CDS             801..2249
                     /locus_tag="VIL39_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5480933.1"
                     /translation="MAERKKSTFQRLQDGRLNRKQRKELAQRLTKDDPGLEVVHRDAA
                     GIDIGNASHFVAVPAGRDAEPVQEFGSWTGELRRMAEWLNKCGIRTVVMQSTGVYWIA
                     AHDVLEQAGFEVWLVNARGTKNLPGRKSDVQECQWLMKLHSYGLLRNSFRPPEQIRAL
                     RTVWRLRDRVVSDAGRTIQQMQKALTKMNVHLANAINDVSGVTGLRIIRAIVSGQRDP
                     RQLAKLRDVRVRASEEEIACSLEGNWQSDVLFELEQALETYDFHQKQLAACDAQLQQY
                     MAALPDRERVPAAEPVAEPQEPAGKRRKAKSKRKAKNYPSFDLAAELARKTGVDLTTI
                     DGINVLTAQTIFAELGADLSAFPSEAHFCSWLTLAPKRDVSGGKVIKHVSSPMQNRVA
                     LALRMAAQSLFNSNSYLGAKSRQLRARLGGLKAIKAMARYLACLVYRLLTKGQAWVDR
                     GTAHFEEQRKFRDMAALGRRAAAMGLRLVPNA"
     gene            2336..2485
                     /locus_tag="VIL39_05595"
     CDS             2336..2485
                     /locus_tag="VIL39_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480934.1"
                     /translation="MTRFEYDAGRAATARANFKKAGVDQVAMVVQDDADKKGYTDYLT
                     KLQPL"
BASE COUNT          538 a          665 c          864 g          424 t
ORIGIN      
        1 ccaccgcgcc tgccattcga gcatcgaccg tagagtcctg atcccaaagg tctctccaga
       61 agtgtgaaag gacaccgtga cgcggcggcc atcggggtgc cggtataacc ggtgggcacc
      121 ggactggcga tcgagagaga aaccatcccg aagcaaagcg gctatgacct cgcgagcggt
      181 cagtgtgcga aggcaggagt agtccagcgc catcggctaa acgatcacgg caacctgggg
      241 ttcggctagc acctgcactt gatcggaggg cgttgaaggc agagcctcgc cgtgctcaac
      301 gaggctgtcg accaccatgc gaaccacgtc ctgaatgttg cgcagggcct cagtgcgcgt
      361 gtggccccag gtggcggcgc cagccgagac cagtgcgggg cagtaggcat gccagcggtc
      421 cccgtcgggc tcaacgatca ccttaaagtt gtaggtcatg ctgagaacct tcccgctcac
      481 cagtatgact gaaaacagag ggcagggaag attcaccaca gagcggtggc cccggagggg
      541 ctccggggtc cagtgaacaa ggtaactttg ctttaggcag ggcgattgta gaacactgga
      601 cgagccatgc tcggcaagct atggtcgtta gattcttggt gcccttcgtg cccgtcacag
      661 agcctgacct cggttgcggc gatccattgc ttggtggcga gttgtcctga aaaaggccaa
      721 gctcgacccc gctggagaga ctgtgaagtg attgccaacc gagccgagcc tggcaaattc
      781 aaaaacaaag agagtatgcc atggcagaac gcaaaaagtc cacctttcag cggttgcagg
      841 acggaaggtt gaatcggaag caacgcaagg aactggccca acggttgacg aaggacgatc
      901 cggggttgga ggtcgtgcat cgcgatgcgg cgggaatcga tattgggaac gcgagtcatt
      961 tcgtggcggt accggcgggc cgggatgcgg agccggtgca ggaattcggc tcctggaccg
     1021 gggagctgcg ccggatggcg gaatggctga ataaatgtgg aatccggacc gtggtgatgc
     1081 agtccacggg agtctactgg atagcggcac acgacgtgtt ggaacaagcg gggtttgagg
     1141 tttggctggt gaatgcgcgc ggcaccaaga acctgccggg gcgcaagagc gatgtgcagg
     1201 agtgccagtg gttgatgaag ctgcatagct acgggctgct gcggaattcg ttccggccgc
     1261 ccgagcagat tcgagcgttg cggacggtgt ggcggctgcg ggaccgggtg gtgagcgatg
     1321 cgggccggac catacaacaa atgcagaagg cgctcacgaa gatgaacgtg cacctggcca
     1381 acgcgatcaa cgacgtcagc ggggtcaccg gattgaggat catccgggcc atcgtgagcg
     1441 ggcaacggga cccgcggcaa ttggccaagc tgcgggacgt gcgcgtgcgt gccagcgagg
     1501 aagagatcgc ctgcagcctg gaaggcaact ggcaatccga cgtgctcttc gagttggagc
     1561 aagcgctgga aacctatgat ttccaccaga agcaactcgc cgcctgcgat gcgcagttgc
     1621 agcagtacat ggcggcgttg ccggatcgtg agcgggtgcc ggctgccgaa ccggtggctg
     1681 agccgcagga gccggcgggc aagcggcgca aggccaagtc gaaacggaag gcaaagaatt
     1741 acccgagctt cgatttggcg gcggaactcg cgcgcaagac gggcgtggat ttgacgacca
     1801 ttgacgggat caacgtgctg acggcgcaga cgatattcgc cgaactgggg gcggatctga
     1861 gcgcgtttcc gagcgaagcg catttctgct cctggttaac ccttgcgccc aagcgagacg
     1921 tgagcggggg aaaggtgatt aagcacgtgt caagcccgat gcaaaaccgg gtggccctgg
     1981 ctttgcgaat ggcggcccaa tcgttgttca acagcaatag ttatctcggc gccaagtccc
     2041 gtcagttgcg ggcgaggctg ggcggcctca aggcgataaa ggccatggcg cgctatctgg
     2101 cctgcctggt ctaccggctt ctcaccaagg ggcaagcttg ggttgaccgg ggaaccgccc
     2161 acttcgaaga gcagcgcaag tttcgggaca tggccgcact cggacgccgg gccgctgcca
     2221 tgggcctccg tttggtgccg aatgcgtagc gacatccgag ccctgtagca gaacattttc
     2281 cgtccctcgg gagtgagtta ctggagagag gtggagaaga aggtgctctc ggtcattgac
     2341 gaggttcgag tacgacgccg ggcgcgccgc gacggctcgg gcgaacttca agaaggcggg
     2401 cgtcgatcaa gtggcgatgg tggtccagga cgacgcggac aagaaaggat atactgacta
     2461 tctgaccaag ttgcagccgc tgtagagggt c
//
LOCUS       DATCGS010000272         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_73903, whole genome shotgun sequence.
ACCESSION   DATCGS010000272 DATCGS010000000
VERSION     DATCGS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73903"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..955
                     /locus_tag="VIL39_05600"
     CDS             86..955
                     /locus_tag="VIL39_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5480935.1"
                     /translation="MNKNILGLSLLLSTVLLTPAVAGEPARAKTNAFFAFCIDTHDAK
                     KRNLEQQAALLKELGYDGVGHLWLNDIPERLKTLDAASLKLFQITMTVDITPGKQAYD
                     PKFKEVMPLLKGRGVQFVLLMNGLKPSDPAGDARAVEIVREMASLASDSGAQILLYPH
                     SYMWMERIEDAVRVADKVDRPNVGVMFNLCHWLRVSKDRNYKPLLASAMPRLWAVSIS
                     GADEFDDKPGWAHYIQPLGRGSFDMLAFLKTLRQLGYTGPIGLQCYGIPGDVRDNLSE
                     SMKVWRSYAAKMN"
     gene            complement(1003..1875)
                     /locus_tag="VIL39_05605"
     CDS             complement(1003..1875)
                     /locus_tag="VIL39_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5480936.1"
                     /translation="MKRALSIIGVIGVIGVITFLAITRQMAQGRAARLDRALMDAEAR
                     VIQLEADIETAKQRSPELTQATGASSGAGGSTIAPPAAAGVAGPLVRYVAISKPGLTN
                     LVRIEGTSTVHDWQVESHLIGGSAELGTGLPTRSGAQSLPGIADAKVNAFIPVRSLKS
                     VEKNGSPYSDPMDEIMYGKLLDQTNKRITYTLTSLILKEQPRQMADPVLYEATGNLCV
                     AGVTNVITMPVTALPDAAGRIQFAGSVKVKMTDFKISPPSPSLGGVSIKTGDEVTLRF
                     VWWVNRVSPVQSAK"
     gene            complement(1971..>4142)
                     /locus_tag="VIL39_05610"
     CDS             complement(1971..>4142)
                     /locus_tag="VIL39_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY5480937.1"
                     /translation="YLAETNPAAAKTEYELIKANAAYPEVHRYEAAECVKEIDRAAKG
                     LPARDVTASRTKIPPITVFAVEYFVAPQGNDANPGTRGKPFATLEKARDTIRALKAKG
                     ALPGPVCVRLLPGEYPVERTFELTAADSGTEAAPIVYRADRKGAAVLYGGRRLNGFVP
                     VTDPVVLDRLPAEARGKVFQCDLKKAGVTNYSSLTERGYGVNPPPLTLEVFFNGAPLT
                     LARWPNTGFINGGKIVQPGSKSADKPSVFEYLDDRHARWANAEDAWLFGYFRHGWADR
                     TLKVRSIDPAAKQIACGPYDLSGENMEPVKWFNQGRIKYFAFNLLEELDQPGEWYLDR
                     KTGILYCYPPADPAKAVVEIGMLSAPMLTLTRASQVRLEGLVFDLSRADCMSLQDCEG
                     CLVAGCTVKRFAGSGISIHGGRGDGILGCDLYSLGRGGTEVTGGDRQTLTPAQHFVEN
                     CLMHSLGRLDHTYVPGIGMAGVGIRAAHNRFYDCPSSAIRFDGNDLLMEYNQVERVVL
                     ESEDQGGMETYGNPTFRGNVLRYNSFAFIGVATAMEGPAGRAGIRLDDTISGTLVYGN
                     IFFHASQGFGGININGGRDNIIDNNIFAECEKGITGNYDANNALWKALGKHPAFIMSE
                     LYLKRYPGLLRLHEPPALNSAWRNVFWKCGPLFTTYNQPSTQKFDLLANAEYAAADPG
                     FVDAAKGDFRLKPDAELLRRIAFRPIPVEEIGLYQDQYRAD"
BASE COUNT          776 a         1274 c         1282 g          810 t
ORIGIN      
        1 agctcgccac cgaggccctg gcccgcacca aagaccgcga gcgagcgtgg atagtctaag
       61 gcgtggctgg taatagatga attatatgaa caagaacata ttaggattaa gcctgcttct
      121 gagcaccgtc ctgttgaccc cggcggtcgc cggagaacct gcgcgtgcga agacgaacgc
      181 gttcttcgct ttctgcattg acacgcacga cgccaagaag cgcaatctgg aacagcaagc
      241 cgcgctgctc aaggaactcg gctacgacgg agtcggccat ttgtggctga acgacatccc
      301 tgagcggctt aagacgctcg acgcagccag cctcaagctg ttccagatca ccatgacggt
      361 ggacatcacg cccggcaagc aggcctacga tccaaagttc aaggaggtca tgccgctgct
      421 caaagggcgc ggagtgcagt ttgtgttgct gatgaacggg ctgaaacctt ccgatcccgc
      481 cggtgatgcc cgggcagtcg agatcgtccg cgagatggct tccctggcca gcgactcggg
      541 cgcccaaata ctgctctatc cgcatagcta catgtggatg gaacggatcg aagacgcggt
      601 ccgtgtggcg gacaaggtgg accggccaaa cgtgggcgtg atgttcaacc tctgccactg
      661 gctgcgggtg agcaaggatc gcaattacaa gccgctgtta gcttcggcga tgccccggct
      721 ttgggcggtc tcgatcagcg gcgccgacga gtttgacgac aagcccggct gggcgcacta
      781 catccaacct ctgggccgtg gctcgttcga tatgctggcc tttctgaaaa cgctgcgcca
      841 gcttggctac accggcccga ttggtttgca gtgctacggc atcccgggag acgtgcgcga
      901 taacctctcc gaatccatga aggtctggcg cagttacgcc gccaaaatga attgaggttg
      961 caacttcagt ggtggcctcc tgttcgtgcc ctcgcctcgg gctcacttgg cggactgcac
     1021 ggggctaacc cggtttaccc accagacaaa cctaagcgtc acctcatcgc cggtcttgat
     1081 ggacacccct ccgagggatg gcgagggcgg ggagatcttg aagtcggtca tcttcacctt
     1141 tactgacccc gcaaactgaa ttctgccagc cgcgtctggc aacgcggtaa ccggcatggt
     1201 gatgacgttc gttacgccgg ccacacagag attcccggta gcctcataca agacagggtc
     1261 agccatctgg cgcggctgct ctttcagtat cagcgaagtc agcgtgtaag tgatccgttt
     1321 gttggtctgg tcaagcagct tgccatacat gatttcatcc atcgggtcgc tatacgggct
     1381 gccgtttttc tccacgctct tgagcgagcg caccgggatg aacgcgttga ctttggcgtc
     1441 cgcgataccc ggaagggact gcgccccgga ccgtgtgggc agtcctgtcc ccagctcggc
     1501 gctgccaccg atgagatgac tttcgacttg ccagtcgtga acggtagaag tgccctcgat
     1561 ccgcaccagg ttcgtcagcc cgggcttgga gatggccacg tagcgaacca acggtccggc
     1621 aaccccggcc gccgcgggcg gcgcgattgt gctcccgccc gcgccagagg atgctcccgt
     1681 ggcttgtgtc aactcgggcg accgctgctt ggcggtttca atatccgcct ccaactggat
     1741 cacccgcgct tccgcgtcca tcaaagcccg gtcgagccgg gcggcccgtc cctgcgccat
     1801 ttgccttgtg attgccaaga aggtgatcac gccgattacg ccaatcacgc cgatgatgct
     1861 gagtgcccgc ttcatagttc cttgtcttgg acgaccttgc cccggcgggg tattcactgc
     1921 aaagcggaac acaagcagcc gggcgacggc ctccagttag cttccaagaa ctaatcggcg
     1981 cgatattggt cctggtagag gccgatctcc tccacgggaa tcgggcggaa ggcgatgcgg
     2041 cggaggagtt cggcatccgg tttcagccgg aagtcaccct tggccgcatc cacgaagccc
     2101 ggatcagcgg cggcatactc cgcattggcc agcaagtcga acttctgagt tgagggctgg
     2161 ttgtaggtgg taaacaaggg gccgcacttc cagaatacat tccgccaggc gctgttcaag
     2221 gccggcggct catgcaggcg cagcaggccg ggataccgct taaggtaaag ctcggacatg
     2281 ataaacgccg ggtgcttccc cagagctttc cagagcgcat tattggcatc gtagttgccg
     2341 gtgatcccct tttcgcattc ggcgaagatg ttgttgtcaa tgatgttgtc ccggccacca
     2401 ttgatgttga tgccgccgaa cccttgggaa gcgtggaaga agatgttgcc ataaacgagc
     2461 gtgccgctga tcgtgtcatc caggcggatg cccgcgcgcc cggccggccc ctccatagcc
     2521 gtcgccacgc cgatgaaggc aaaggagttg tagcgcagca cgtttccgcg gaaagtgggg
     2581 ttgccatacg tctccattcc gccctggtcc tcgctttcga gcacgacgcg ctcgacctgg
     2641 ttgtactcca tgaggagatc gttgccgtcg aaccggatcg cgctggacgg acagtcatag
     2701 aagcgattgt gcgccgcccg gatacccacc ccggccatgc cgatgcccgg gacataagtg
     2761 tgatccaggc ggcccaggga atgcatcagg cagttttcca cgaagtgctg cgccggggtc
     2821 agggtctggc ggtcgccccc cgtgacttcg gtgccgcccc ggcccaggct gtacagatcg
     2881 cagccgagga tgccatctcc ccggccgcca tgaatggaga tgccgctgcc ggcgaagcgt
     2941 ttcaccgtgc aaccggccac caggcagcct tcgcaatcct ggagcgacat gcaatcggcg
     3001 cgggaaagat cgaagaccag gccctccagt cgcacctggg acgccctggt gagggtcagc
     3061 atgggggcgg ataacatacc aatctccacc acggctttgg cggggtcggc gggaggatag
     3121 cagtagagaa tccccgtttt gcggtccaga taccactcgc ccggctggtc cagctcttcg
     3181 agcaggttga aggcgaaata cttgatcctg ccttggttga accatttgac gggctccatg
     3241 ttctcgccgg acaggtcgta cggtccgcag gcaatttgtt tcgcggcggg gtcaatgctt
     3301 cggaccttga gggtccgatc ggcccagccg tggcggaaat agccaaacag ccaggcgtcc
     3361 tctgcgttgg cccaccgcgc gtggcggtcg tccaggtact cgaacacgga tggcttatcc
     3421 gccgacttcg agccgggctg aacgatcttg cctccattga taaagcccgt attcggccag
     3481 cgggcgaggg tgaggggcgc gccgttgaaa aacacttcca gcgtcaaggg gggcggattg
     3541 acgccgtagc ctcgctccgt gagcgagctg taattcgtga cgccggcctt cttcaggtcg
     3601 cactggaaaa ccttgccgcg cgcttcggcg gggagacggt cgagaacaac ggggtcggtg
     3661 accgggacga atccgttaag ccgcctgccg ccataaagca cggctgcgcc cttcctgtcc
     3721 gcgcgataga cgatgggggc tgcctccgtg ccggaatcgg cggcggtcag ctcgaaggtc
     3781 ctttccactg gatactcgcc cggcagcaga cgcacacaaa ccgggccggg aagcgcgccc
     3841 ttggctttga gcgcgcggat cgtatcgcgg gccttctcca gcgttgcgaa cggctttccc
     3901 ctggtaccgg gattcgcatc gttgccctgt ggagctacga agtactccac cgcgaacacg
     3961 gtgatcggcg ggatcttcgt ccgcgaggcc gtgacatcgc gggcgggaag tcccttggcc
     4021 gcacggtcaa tctccttcac gcattccgcc gcctcgtagc ggtggacctc ggggtacgct
     4081 gcattggcct tgatcagctc atactcggtc ttcgccgctg cggggtttgt ttccgccagg
     4141 ta
//
LOCUS       DATCGS010000273         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_420334, whole genome shotgun sequence.
ACCESSION   DATCGS010000273 DATCGS010000000
VERSION     DATCGS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420334"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VIL39_05615"
     CDS             <1..1046
                     /locus_tag="VIL39_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5480938.1"
                     /translation="PEEAAQVLGFIDQARLDLPQVFALLNALGDGLHQAGSSLALMDP
                     QNRLQRFYSQASDVVANSLVFDPIRVEAIRLMGVGPYTVSSMGDLLLLPLGSGQSEAI
                     QSAAIATLGRFNDSRVSPALIQRWRVLTPRLRNEAITALLARSSRVGAVLTALETGRI
                     DRADLSPAQVNFLRTHRDPATCQRALQLFGPGPRQHPEAVRRFGPALGLKGAAERGRE
                     TFVARCAACHQRGGASQGIGPDLVSARIYGKGRILAAILEPNAEVWRDFLPYVIETTE
                     GKTLIGLLRGDNAATVSLQPLNGRPVVLPRANIQDLQAQPWSLMPERLEEGLTPQGMA
                     DLLEYILRPTATP"
     gene            complement(1165..2820)
                     /gene="leuA"
                     /locus_tag="VIL39_05620"
     CDS             complement(1165..2820)
                     /gene="leuA"
                     /locus_tag="VIL39_05620"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5480939.1"
                     /translation="MLKDPSAKYRPFPPVHLPDRQWPNRTLTHAPSWCSVDLRDGNQA
                     LAVPMNVGQKLELFQTLVKCGFKEIEAGFPSASNTEFTFNRRLIDEHRVPDDVWLQVL
                     VQAREDLIERTFESLVGAKKVIIHLYNSTSPAQRRIVFGMSKEEILRVAVRGAQWVQE
                     RLPRLKGSEVRLQYSPESFSATEVEFAKEASEAVMAVWQPTPQNKMILNLPDTVEVAT
                     PNVYADQVEWICRNIKNRDSLIVSLHAHNDRYTGVAATELGLLAGGDRVEGTLFGNGE
                     RTGNLDVVTVALNLYMHGIDPKLDFSDLNGICEVYERCTGMTVPPRHPYAGELVFTAF
                     SGSHQDAIKKGLAEWETGRQKYWEVPYLTIDPADIGRQYREVIRVNSQSGKGGVAYLL
                     ENEYGIVLPKDMQREFGPIANEEVDRLGREVSGAELKGMFWREYIERAAPWQLQGFET
                     ESRDGMVRCRARLLRGGKPVVLSGEGNGPLAALVHGFSTAGVPRFEIANYSEHALSSG
                     EEASAIAYIQIKHSDGRTRWGAGVDTNIELASVRAVLSALNRS"
BASE COUNT          545 a          997 c          999 g          588 t
ORIGIN      
        1 gacccgagga agccgcccag gtgctgggtt tcattgatca ggcgcggctt gacctgccgc
       61 aggtattcgc gttgcttaac gctctgggag acggtctgca tcaggcgggc agttcgctgg
      121 ccctcatgga cccgcaaaac cgcctccagc gattctactc ccaggcctca gacgtggtgg
      181 cgaactccct ggtctttgac ccaatccggg tcgaggccat ccgattgatg ggcgtcggcc
      241 cttacacggt ctccagcatg ggcgatttgc ttctgctccc gctgggttcg ggccaatccg
      301 aggctattca atccgccgcc atcgccaccc tgggccgctt caacgattca cgagtatccc
      361 cggccttaat tcagcgctgg cgagtattga cgccgcgcct gagaaacgag gcgataaccg
      421 ccctgctggc acggtctagc cgcgtcgggg ccgtcctgac ggcgctcgaa accgggcgga
      481 ttgatcgcgc cgatttgtca cccgcgcagg tgaatttcct gcggacgcat cgcgacccag
      541 ccacctgtca gcgcgcgctg caactgttcg gccccgggcc caggcagcat ccggaagcgg
      601 tgcggcgatt cgggccggcg ctgggcctga agggtgccgc cgagcgtgga agggaaacct
      661 ttgttgcgcg ctgtgccgct tgccaccagc ggggcggcgc aagccagggg atcggccccg
      721 atttggtaag cgcgagaatc tacggcaaag gaagaatcct cgcggcaatc ctggagccaa
      781 atgctgaagt atggcgcgat ttcctgccct atgtgatcga aaccacggaa gggaagacct
      841 tgatcggcct gctgcgcgga gacaacgcgg cgaccgtctc cttgcagcca ctgaacgggc
      901 gtcccgtggt gttgccgcgc gccaacattc aagacctgca ggcccagccg tggtccctca
      961 tgcctgaaag attggaggag gggctcacgc cgcaaggcat ggctgacttg ctcgagtaca
     1021 ttcttcggcc tacagccact ccctaatctt ctcccggtac ttgctgcaga tgaacccaag
     1081 caccagcagc acgctcacct gtgagagcac ggcggggagg gggccagggt tacgattgcc
     1141 caaagccctc cggcactgac cccgtcacga ccggttcaag gcgctgagca cggctcgcac
     1201 ggaggccagt tcgatattgg tgtccacacc ggcgccccag cgggtgcggc cgtcgctgtg
     1261 cttgatctgg atgtaggcaa tggccgacgc ttcttccccg gaactcaggg cgtgctcgct
     1321 gtaattcgcg atttcaaagc gcggcacgcc cgcggtgctg aacccgtgga ccagcgccgc
     1381 cagcgggccg ttgccttcgc cggacaacac gaccggcttg ccaccgcgga gcaaccgcgc
     1441 gcggcagcgg accatgccgt cgcggctctc ggtttcgaag ccctgaagct gccatggagc
     1501 ggcgcgttcg atgtattcgc gccagaacat gcccttcaac tcggcgccgc tgacctcgcg
     1561 gccgagccgg tccacttcct cgttggcgat cgggccgaac tcgcgctgca tgtccttggg
     1621 cagcacgatg ccgtactcgt tctccagaag gtaggcgacg ccgcccttgc ctgactggct
     1681 gttgacgcgg ataacctcgc ggtattgccg gccgatgtcg gctgggtcga tggtcagata
     1741 tggcacctcc caatatttct ggcggccggt ttcccactcg gccaggccct tcttgatcgc
     1801 gtcctggtgc gagccgctga aggcggtgaa gaccaattcg ccggcgtagg ggtggcgcgg
     1861 cggcaccgtc atgccggtgc agcgctcata gacctcgcag atgccgttga ggtcagagaa
     1921 gtcgagcttc gggtcaatgc cgtgcatgta gaggttgagc gcgacggtca cgacgtcgag
     1981 gttgccggtg cgctcgccgt tgccgaacag ggtgccttcg acgcggtcgc cgccggcgag
     2041 caggcccaat tccgtggccg ccacgccggt gtagcggtca ttatgggcgt gcaaactgac
     2101 gatgagcgag tcgcggttct tgatgttccg gcagatccac tccacctggt ccgcatagac
     2161 gttcggcgtg gcgacctcga ctgtgtcggg caggttcagg atcatcttgt tctgcggcgt
     2221 gggctgccag acggccatca cggcttcgga agcttccttg gcaaactcga cttcggtggc
     2281 gctgaagctt tcgggcgagt attggagccg gacttcgctg cccttgagcc ggggcaggcg
     2341 ctcctgaacc cattgggcgc cacgcacagc cacgcggagg atctcctcct tggacatgcc
     2401 gaaaacgatc cggcgctgcg cgggggaggt cgagttataa agatggatga tgaccttctt
     2461 cgcgccgacc agcgactcga aggtgcgctc gatcaaatcc tcccgcgcct gcacgagcac
     2521 ctggagccac acgtcgtccg ggacgcggtg ttcatcgatc agccggcggt tgaaagtgaa
     2581 ctcggtgttc gaggccgacg ggaagccggc ctcgatttcc ttgaagccgc acttcacgag
     2641 cgtctggaac agctcgagct tctggccgac gttcatcggc acggcaagcg cttggttgcc
     2701 atcgcggagg tccacgctgc accaactcgg tgcgtgcgtg agggtgcggt tcggccactg
     2761 gcggtcgggc aagtggaccg gcgggaacgg acgatatttg gcagacggat ctttaagcat
     2821 ggtcgtttcg ttttcttgtt aacaccgccc ggcggtcagg caaataaaaa accccaccgc
     2881 cggttgcggc agtggggttt agtaaacttt cgtgaaaggc tacaacccaa ctgccgcgcg
     2941 gctcagcagc gcgctcgata gcagcaggtt taaatcgcaa ttcacgccgc caatgtagct
     3001 acccggccag caggggtcaa acggtttctc ggcctcgtgc cggatgttct ctgaacttgc
     3061 ttgcgctctc ccaagcgcgc gggaacgccc accgcgcgca acgccgcgac atacaccgcc
     3121 tcgaaaccg
//
LOCUS       DATCGS010000274         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_369878, whole genome shotgun sequence.
ACCESSION   DATCGS010000274 DATCGS010000000
VERSION     DATCGS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369878"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIL39_05625"
     CDS             <1..198
                     /locus_tag="VIL39_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480940.1"
                     /translation="FRDDEDRQKLLATLGEACRKTEWPAVGPFLPRRAICNLENLEGG
                     YKPPLGPKDAWWGRWARIWAV"
     gene            complement(914..1204)
                     /locus_tag="VIL39_05630"
     CDS             complement(914..1204)
                     /locus_tag="VIL39_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY5480941.1"
                     /translation="MSTISVNDLKKKPAKQWLKSASKGDLVITSKGQPVAVLLRIAAA
                     SVDSTRALLRSVRALQAQTALQQAAVVNGTSDFSMSDVDAEIAAARRARRQK"
     gene            complement(1401..2201)
                     /locus_tag="VIL39_05635"
     CDS             complement(1401..2201)
                     /locus_tag="VIL39_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5480942.1"
                     /translation="MSATIKLSWPATKGFWEIPVLHEDEHLLALDKPSDLLTSPDSTE
                     PDRPDLVTLLHAGIERGAPWAAQGGRAYLMPAHRPDAETSGVLLLAKSKPVLTTLMDW
                     FSAEKPGDRHLALVQGTPPEDQFTVDAKMTRHPLRAGFMRVDSQHGKRSRTVFEVREK
                     FAGYTLLQCDALTHRLHQIRVHLRYLGLSAVGDMAYGGRPLLLSSLKPNYRLKPDQIE
                     HPLMSRAAIHAEVLTLPHPITGETVTITAPWPKDLTVAVKYLRRYAIA"
     gene            complement(2252..>2879)
                     /locus_tag="VIL39_05640"
     CDS             complement(2252..>2879)
                     /locus_tag="VIL39_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480943.1"
                     /translation="ATKAFVRTSEDPFAGPILGMDGVYVIALNKKLPSEIPTLDRIRD
                     RVVQDYKQNQALNLARQAGTVFYSTLTNGLAQGKSFASVCLSAQLKPVSVTPFSLSTR
                     ELPEVEEHLTINQFKQISFTTPLGKPSPFQPTADGGLVVYVKEKMPLDEAKMNTTMPA
                     FANYVRQNRQSEAFNEWFRKEAEKGLRDTPIAKPPAPPEMTSAPKAKK"
BASE COUNT          527 a          859 c          924 g          569 t
ORIGIN      
        1 ttcagggacg acgaggaccg gcagaagctc ctggcgacgc tgggtgaggc ttgccgcaag
       61 accgagtggc ctgcggtcgg ccccttcctg ccccggagag cgatttgtaa cctcgaaaac
      121 ctggaggggg gatacaaacc gcccttgggc cccaaagacg cctggtgggg gcgctgggcg
      181 cgaatttggg ctgtttagct tcggctgggg tcgggcaggg agggtatagt tgttacggac
      241 atttcccttg gggctgacgc caagttcacg ggctggcgga ctctgcccag acgcaatccg
      301 ctggaacgta gagtgcgggc gcggcgttca aagttcatct ggcaatttct ctgtcccgct
      361 gccgcgcctg cctgccacca ggtctccgtc tgcagccccc aggtgaaccc accatgcagg
      421 tgcatgtaat ctggcccgcc agcctgcccg acgaacgcat cgctccactg cgcatacgtg
      481 atgcaagtcc ggagaaccgg ccgactgaag aatgggttgc ccaaggccac ttgcggcggc
      541 cagcggacac actttaggtt tgagttttct aagctacgag tttaaaaagc tcaaatgcgt
      601 tacctgcctc gcgagttgga gcggcagatc accccagccc tctcccttgg ggagaaggtg
      661 ccttgccgtc cgttgcgaga gatgcctgca cgcctcggtt tgtcgcagcg cggtgggcag
      721 ggcaagtgcc agtggtcact tttcacgctg caactttcgc cactgctccg tgaagacagc
      781 cggagaaact actgcgcatc ctctgcattt atcgggcggg taatcagaca agttgccagt
      841 gtgagaactt gggcgggatt cccttgagat tggagcaagc ccgacacgac gatgttggtg
      901 tcgagcacga ttctcatttc tgacggcggg cgcgacgggc agcggcgatt tcggcatcca
      961 cgtcggacat ggagaaatcg gatgtgccat tgacaacggc ggcttgctga agcgcggtct
     1021 gcgcttgaag agcacggaca ctacgcaaaa gtgcgcgggt ggagtccaca gacgccgccg
     1081 cgatgcgcag caacacggcg accggctggc ctttggaagt gatgaccaag tcgcccttgc
     1141 tggctgactt gagccattgc ttcgccggct tcttcttcag gtcgttgacc gaaatcgtgc
     1201 tcatgccgaa agaataggct ggtccgccaa ttcgtgcaag cgcggctcag ccgcagggat
     1261 gcggtcatgt cgcggacaga agtgattacg catcaggcca aagggcagtt gaacctcaga
     1321 acatccgccg ctccatctgt atcccgaact ccctccggcc cgcctggctg gcctgggttt
     1381 gaacgaccga agatgcgggg ctacgctatc gcgtaccggc gcagatactt caccgccacc
     1441 gtcaggtcct tcggccacgg ggcggtgatg gtcaccgttt cgccggtgat gggatggggc
     1501 agggtaagga cttcggcgtg gatcgcggcc cgggacatca gtggatgctc gatctgatcc
     1561 ggtttgagcc gatagttcgg tttcaagctc gaaagcagca gtggccggcc gccataagcc
     1621 atatccccga cggccgacag cccgaggtaa cggaggtgga cccgaatctg atgcaggcga
     1681 tgagtcaatg cgtcgcactg caggagggtg taaccggcga acttctccct gacctcgaag
     1741 acggtccggg agcgtttgcc gtgctgcgag tccacgcgca tgaagccggc ccgtaacggg
     1801 tgacgcgtca tcttggcgtc caccgtgaac tggtcctccg ggggggtgcc ctgcacgagc
     1861 gccaggtgcc gatcgccagg cttctccgcg ctgaaccagt ccatcagggt ggtgagcact
     1921 ggtttgctct tggccagcag cagcacgccg ctcgtctcgg catcaggccg gtgcgcgggc
     1981 atcaggtaag cccggccgcc ttgcgccgcc cagggcgcgc cgcgctcgat gcccgcgtgc
     2041 aacaacgtca ccaggtccgg gcggtccggc tcggtgctgt cgggggaggt cagcaagtcg
     2101 ctcggcttgt cgagcgccag caggtgctcg tcctcatgca gaacagggat ttcccagaac
     2161 cctttcgtcg cgggccatga aagtttgatg gtggcactca cagtcgcata gcagagaacc
     2221 acagataaac acagcggctc aggccgggcc actatttctt cgccttcgga gccgacgtca
     2281 tttccggcgg ggccggtggc ttggcgatcg gtgtgtcgcg caggcccttc tcggcttctt
     2341 tgcggaacca ttcgttaaag gcttcgcttt gacggttctg gcggacatag ttggcaaaag
     2401 caggcatggt cgtgttcatt ttcgcctcgt ccagcggcat cttttctttc acatagacaa
     2461 cgagcccgcc atcagcggtc ggctggaagg ggctgggctt cccgagcggc gtggtgaagg
     2521 agatttgctt gaattgattg atcgtgagat gctcctccac ttccggcagt tcccgggtgc
     2581 tgagcgagaa cggcgtcacg gacacgggct taagctgcgc gctcaggcat acggacgcaa
     2641 acgatttccc ttgcgccagc ccgttcgtca gcgtcgagta aaagactgtg cccgcctgac
     2701 gcgccagatt cagcgcctgg ttctgcttat agtcctgcac cacccggtcg cgaatccggt
     2761 ccagggtcgg gatctcgctc gggagctttt tgttcaaggc gatgacatac acgccgtcca
     2821 tgccgagaat cggcccggca aacgggtcct cggacgtccg gacaaaggcc ttggtcgcg
//
LOCUS       DATCGS010000275         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_618676, whole genome shotgun sequence.
ACCESSION   DATCGS010000275 DATCGS010000000
VERSION     DATCGS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618676"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VIL39_05645"
     CDS             <1..952
                     /locus_tag="VIL39_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HEY5480944.1"
                     /translation="GGGMGRSHGNAATFPRLADLIGFLPPEKVVDVARAVLTIHRDFS
                     DRADRKHARLKYVLEDRGAQWFREELERRVNFSLAEPMPFKFEKQGDAFGWHQQADGR
                     LFLGLFVETGRIRDHDGWRMKTALREAVAKYQPEVHLTPANNVILANLPAAQQDEITR
                     LFAEHGVQTERDGQGSSLRRASMACVSLPTCGLGLAESERYLPGLITRLERLLAELGL
                     GGQEITIRMTGCPNGCARPYMAEIGFVGKAPGRYQVWLGGNEASTRLNRLYRDLVKDP
                     DIVTELRPLFARYAQERLTDERFGDWVARVLWPEQPAAAK"
     gene            959..1615
                     /locus_tag="VIL39_05650"
     CDS             959..1615
                     /locus_tag="VIL39_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY5480945.1"
                     /translation="MTPDQLACWNAELRCKSPLEITRWAIAYAGGRAVVSTNFRPYEA
                     AILHLCVQAQPDIPVLWVDHGYNRSATYKHAEQLCSLLKLNLRVFLPRISAAHRDAAW
                     GPIPALDDESGLKQFSALMKLEPFQRGMKELAPKVWITALRQVQNSNRAGLDIVSADP
                     NFGTLKVSPVFYLSDADMETYLQEHELPNEWDYFDPAKADDKRECGLHAAWGGQLVRA
                     "
     gene            1657..>2279
                     /gene="cysD"
                     /locus_tag="VIL39_05655"
     CDS             1657..>2279
                     /gene="cysD"
                     /locus_tag="VIL39_05655"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5480946.1"
                     /translation="MHRYHLDHLQYLETEAIHVMREVAAGFERPALLFSGGKDSICIL
                     RLAEKAFRPSDIPMPLLNIDTGHHFPELNEFRDRRAAELGCKLIIRTVERAIANGTAV
                     PAPGEVSRNRLQIPTLLAAIEEFRLDCCIGGARRDEEKARAKERFFSFRDSFGQWDPK
                     NQRPEIWNLYNARLNPGENMRVFPLSNWTEMDVWEYIRKEKLAVPGI"
BASE COUNT          420 a          764 c          719 g          376 t
ORIGIN      
        1 gggaggcggc atgggacgca gccacggcaa cgcggcgacg ttcccgcgat tggcggacct
       61 catcggcttt ttgccgccgg aaaaggtggt ggacgtggcc agggcggtgc tgacgatcca
      121 ccgcgatttc agcgaccgcg ccgaccggaa gcacgcccgg ctgaaatacg tcctggaaga
      181 ccggggcgct caatggttcc gcgaagagct cgagcggcga gtgaatttca gcctcgccga
      241 accgatgccg ttcaagtttg agaagcaagg cgacgcgttt ggctggcacc agcaggccga
      301 tgggcggctg ttcctcggcc tgttcgtcga gacgggccgc atccgggacc atgacggctg
      361 gcggatgaag acggcgctgc gcgaggcggt ggctaagtat cagccggaag ttcatctcac
      421 cccggccaac aacgtcatcc tggccaacct ccccgcggcg cagcaggacg aaatcacgcg
      481 cctgttcgcc gaacacggcg tgcagaccga acgcgacggc cagggcagca gcctgcgccg
      541 tgcttcgatg gcttgcgtgt cgctgccgac gtgcggcctg gggctggccg agtcggagcg
      601 ttacctgccc ggcttgatca cgcgcctcga acgcctgctc gcggagctgg ggctgggcgg
      661 gcaggagatt accattcgca tgaccggttg cccgaacggc tgcgcgcggc cttacatggc
      721 cgaaattggt ttcgtcggca aagcgccggg gcgttaccag gtctggctcg gcggcaacga
      781 agccagcacg cgcctcaatc ggctctatcg cgacctggtc aaggacccgg acattgtcac
      841 tgagctgcgc ccgctgttcg cgcgctacgc gcaggagcgg ctgaccgacg agcgcttcgg
      901 cgactgggtc gcgcgtgtgc tatggccgga acagccggcc gccgccaaat gattccccat
      961 gactcccgat caactcgcct gctggaacgc cgaactacgc tgcaagtcac cgctcgaaat
     1021 cacccgctgg gcgattgcgt atgccggcgg gcgcgcggtg gtctccacca acttccgccc
     1081 gtatgaagcc gccatcctgc atctctgtgt gcaggcccag cccgatattc cggtgctgtg
     1141 ggtggaccac ggctacaatc gctcggcgac ctacaagcac gctgagcaac tctgctcgct
     1201 gctgaagctg aacctcaggg tcttcctgcc gcgcatctcc gccgcgcacc gggacgccgc
     1261 ctggggcccc atcccggccc tggatgacga gtcgggactg aagcagttca gcgcgctgat
     1321 gaaactcgag ccgttccagc gcggcatgaa ggaactggct ccgaaagtct ggatcaccgc
     1381 tttgcgccag gtccagaact ccaatcgcgc cgggctggac atcgtgtccg ctgacccgaa
     1441 ctttggcacg ctgaaggtga gcccggtatt ttatcttagc gacgcggaca tggaaaccta
     1501 tctccaagag catgagctgc ccaatgaatg ggattacttc gacccagcca aggccgacga
     1561 taaacgcgag tgcgggctgc acgccgcctg gggcggccag ttggtcaggg cgtaatcacg
     1621 catcacgcat cacgcatccc gcatcccgcg aagcccatgc accgctacca tctcgaccac
     1681 cttcaatacc tggagacaga ggccatccat gtgatgcgcg aagtcgccgc cggctttgag
     1741 cggccggccc tgctcttctc gggcggcaaa gactccatct gcatcctgcg cctcgcggag
     1801 aaggcctttc gcccgtccga tatcccgatg ccgttgctca acattgacac cgggcaccac
     1861 ttccccgaac tgaacgagtt ccgcgatcgc cgcgccgcgg agctggggtg caagctcatc
     1921 attcgcaccg tcgagcgcgc catcgccaac ggcacggcgg tgcccgcgcc cggcgaggtc
     1981 agccgcaatc gcctgcagat ccccaccctg ctggcggcca tcgaggagtt ccgtctcgac
     2041 tgttgcatcg gcggcgcgcg acgcgacgag gagaaggccc gcgccaagga gcgcttcttc
     2101 agcttccgcg acagcttcgg ccagtgggac ccgaagaacc agcgcccgga gatctggaac
     2161 ctctacaacg cgcgcctcaa tcccggtgag aatatgcgtg tcttcccgct cagcaactgg
     2221 accgagatgg acgtgtggga atacatccgc aaagagaagc tcgcagtgcc gggcatcta
//
LOCUS       DATCGS010000276         5697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_219931, whole genome shotgun sequence.
ACCESSION   DATCGS010000276 DATCGS010000000
VERSION     DATCGS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219931"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1263
                     /locus_tag="VIL39_05660"
     CDS             229..1263
                     /locus_tag="VIL39_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEY5480947.1"
                     /translation="MLAAPVGHAQPGTADAFAQNRKLGRGVNILGYDPIWRSRDQARF
                     QAKHFRLLKEAGFNSVRINLQPFRQMNAAKDYALSESWFEALDWAVRQAQAQDLRVIL
                     DLHEFTSLGDDPAANKVKFLAFWRQLSAHCQGAPENVLFEVLNEPSRKLTPALWNEYL
                     AEALAIIREKNPTRTVIVGPAFWNSIDHLTELELPAADRHLIVTVHYYTPMDFTHQGA
                     AWAGRKDKLGVDWLGTEKELGALKSDFDKAANWAKQHDRPLFLGEFGAYDKAPMESRV
                     RYLASVARAAEQRGWSWAYWQFDSDFILYDISRDSWIEPIRRALIPAAGPTTSQPSSI
                     STTKKPGESD"
     gene            1425..3704
                     /locus_tag="VIL39_05665"
     CDS             1425..3704
                     /locus_tag="VIL39_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5480948.1"
                     /translation="MTDQKNPAAGTAPESFLGKFTVLFGAVRELWIVFALTILSNLAY
                     RLVNFTLSLWLTSDLGFSDEKTGLAVMIWSAGLTLSIVLVGSLTDALGLRKTFLLGFA
                     LCIISRVFLTFTTVKWIALGGGMALLAVGEGMGAPVTVAALRRYTTTAQRSIAYSVFY
                     AIMNGGFFIALYISDGLRAGMGEHGRFVVPGLGMELSTYRTIFLASLALSVPSLLLTY
                     FWMREGVEATDHGVVITPEQPKYPGLNMFQALGCTVRDTLRETGRIFLGLWQQSGFYK
                     FIAFLSFAAFIKMIFLHMDYTYPKFGIRELGEGAPIGHLYALNSILIVFLAPLVGVLT
                     QKLPAYRMVIFGSFVAASSVFIMTIPSQRFERLADGKMGHFIANSWLGGYSRFSPDDF
                     RDLPAFANQLQSGSNSVSLCLKGSLSPITQALLSRQIANGFRDESLCPHPATALASTT
                     ELRDVPAFLKRLQDDSNLATRPVSQFLWDRFSSKDKSVFGESDPAVEARRAATLAREL
                     NRVLQGDPLYSKKQKARFAGVALSQATRLAVESDPASANDSRVTQSQLLNRLLLEDTY
                     PEFMVKSDCPLQVALAEDITKLMKGPSLFEAQRFAGVPLSDETKQLLAQAPQGGELVR
                     LNRCLLEDAFPADLSKNRVGVPGSVNPWYVMIFLFVVLLSVGEAFYSPRLYEYTAAIA
                     PKGQEASYMAMSSLPFFLAKLGVAPFSGVLLAHFCPDIGPRHPGTLWLLIGLSTMIAP
                     VGLFVFQRFIRVREAGRDE"
     gene            3732..4064
                     /locus_tag="VIL39_05670"
     CDS             3732..4064
                     /locus_tag="VIL39_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480949.1"
                     /translation="MSLTTQIGSAAPTATDSGWSGGLDEAAFYNTVLGVGTIWNHLLV
                     MVGPPTLSYSLAGTQLTLSWPTDVTGFTLEYAESLPATSWTPVSGVVNNQVAVDASSG
                     KRFFRLRK"
     gene            4061..4888
                     /locus_tag="VIL39_05675"
     CDS             4061..4888
                     /locus_tag="VIL39_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5480950.1"
                     /translation="MNTSGSTSIGLSRREDTPRRAFTLIELLVVIAIIAILAALLLPA
                     LAKAKEKAYRIACTNNLKQIGLFLQFYTDDNRDVFPAHRNQNENDNPTTALTNWWGTA
                     IIGYARNQSNVFCCPAIKGKRLDNGVAWSWSFDCHKVGYGINSFFDCLWPYGGASVSI
                     SGITFDSKPWFKRSAVKSPADNLIIGDGMPKSDGTYSSSLWWPNACMDKVKSASQGFE
                     GIDNLRHRNTGVAVFTDCHAEARKDAMINPPVDPGAGDPKGLMNSKYWDPLKRAGDR"
     gene            complement(5209..5379)
                     /locus_tag="VIL39_05680"
     CDS             complement(5209..5379)
                     /locus_tag="VIL39_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480951.1"
                     /translation="MQTRTIEPAHALAAETLSLERTLSDLVNQACALTPAEIALLWQT
                     APPRMPIPPPAT"
BASE COUNT         1054 a         1792 c         1716 g         1135 t
ORIGIN      
        1 ctccccgtag ctggtctggt gtctcgcgcg tgataccgaa gacgaagggc tgcccgtcgt
       61 agaagcgttc acgcagctcg aaggacccgg cccggcaagg tgacccaggc gaaaggtgtc
      121 tctgattagc ccttcaaaga tgccgcctgg tttggtaacc ttgccggcat gaatggaatg
      181 gctcggctcc cactaacgag tccagcactg ttgctggctg caatactcat gctcgcagcc
      241 ccggtcggtc acgcccaacc gggaaccgcg gacgccttcg cgcagaaccg aaaactgggt
      301 cgtggcgtca acatactcgg ctacgatccc atctggcgct cgcgcgacca ggcccggttt
      361 caagccaaac atttccgcct gctcaaagag gccgggttca actcggtgcg catcaacctg
      421 cagccgtttc ggcagatgaa tgcggcgaag gactatgcgc tgagcgagtc ctggtttgag
      481 gcgctcgact gggctgtccg gcaggcgcag gcgcaggatt tacgagtgat cctcgacctg
      541 cacgagttta cttcgcttgg cgatgacccc gcggccaaca aagtgaagtt cctcgccttc
      601 tggcggcagc tctccgcgca ctgccagggc gcgccggaga acgtgctctt tgaagtgctc
      661 aatgagccgt cgcgcaaact gaccccggcg ctctggaacg aatacctggc cgaagcgctc
      721 gccatcattc gcgagaagaa cccgacccgc accgtcatcg tcggaccggc tttctggaac
      781 tcgattgatc atctaaccga actggagctg ccggcggcgg atcgccatct cattgtgacg
      841 gttcactatt acacgcccat ggacttcacg caccagggcg ccgcgtgggc ggggcgaaag
      901 gacaagctgg gcgttgattg gctgggcacg gaaaaagaac tcggcgccct taagagcgat
      961 tttgacaaag ccgccaactg ggccaaacaa cacgaccggc cgctgttcct cggcgagttc
     1021 ggcgcgtatg acaaggcccc gatggaatcg cgggtgcgct acctcgcgag cgtggcgcgg
     1081 gccgccgaac aacgcggttg gagctgggct tattggcaat tcgacagcga cttcattctc
     1141 tacgacatca gccgcgactc ctggatcgag cctatccgcc gcgcgctgat tcctgccgcc
     1201 ggcccaacga cttctcaacc ctcttcaatc agcaccacaa agaagcctgg agaatcagat
     1261 tgagaggcac ccatcggaga tcactacgag cagccactga cactgggcgg agatgcgttg
     1321 ctctaccgcc aacctcctca tgattgggtt gtcggccagc ctcggtcgcg gatttgggcc
     1381 ggcctggtcc ggcggctcgg ggaccactca cagatggact caccatgact gaccaaaaga
     1441 accccgcggc agggactgcg cctgagagtt ttctgggcaa gttcaccgtg ctattcggcg
     1501 cggtgcgcga actctggatc gttttcgccc tgaccatcct cagcaacctg gcctaccggc
     1561 tggtgaactt taccttgtcg ctctggctca cttcggacct ggggttcagc gacgagaaga
     1621 ccggcctggc agtcatgatc tggtcggcgg gattgacgct ttccattgtg ctggttggct
     1681 cgttgaccga cgcgctgggg ttgcgcaaga cgtttctgct cgggtttgcc ctttgcatca
     1741 tctcgcgtgt gttcctgacg ttcacgaccg tcaaatggat cgcgctgggc ggcggcatgg
     1801 cgctgctggc ggtgggagaa gggatggggg ccccggtgac cgttgccgct ctgcgacgct
     1861 acaccaccac cgcccaacgc tccatcgcgt actctgtctt ctacgccatc atgaacggcg
     1921 gcttcttcat tgccctctat atttccgatg gcctgcgcgc cggcatgggt gagcatggcc
     1981 gattcgtggt gcccgggttg gggatggagt tgagcaccta ccgcaccatc tttctcgcca
     2041 gcctggcact gtcggttccg agcttgctcc tgacgtattt ctggatgcgc gagggcgtcg
     2101 aggcgaccga ccacggagtg gtcatcactc ccgagcagcc caaatatccc ggcttgaata
     2161 tgtttcaagc cctgggatgc accgtccgcg acaccctgcg cgagaccggc cgcatattcc
     2221 tcgggctgtg gcagcagtcc ggcttttaca agttcattgc cttcctctcc ttcgcggcct
     2281 tcatcaagat gatcttcctg cacatggatt acacctaccc gaagttcggc attcgcgagc
     2341 tgggcgaggg ggctcccatc gggcatctct atgcgctgaa cagcatcctc atcgtattcc
     2401 tcgccccgct cgtcggcgtg ctgacccaga aacttcccgc ttaccgcatg gtgatcttcg
     2461 gaagcttcgt cgccgcctcg tcggtcttca tcatgacgat cccgtcgcag cgcttcgagc
     2521 ggctggccga tggcaagatg ggacacttca tcgccaattc ctggctgggc ggctactcgc
     2581 ggttttcacc cgatgacttc cgtgacctgc ccgctttcgc caatcaactc caatccggct
     2641 ccaactcggt cagcctctgc ctgaaggggt ccctttcgcc cataacccag gccctgctca
     2701 gccggcagat cgccaacggt ttccgcgacg agagcctgtg tcctcacccg gcgaccgccc
     2761 tggcttccac cacggaactc agggacgttc ccgcctttct gaagcggctg caagacgact
     2821 cgaacctcgc tacccgcccg gtctcgcaat tcctctggga caggttctct tccaaggaca
     2881 agtctgtctt cggggagtcc gatccagcgg tcgaggcccg tcgtgcagca acgttggccc
     2941 gcgaactgaa ccgcgtgctt caaggcgatc ccttgtatag caagaagcag aaggctcgct
     3001 ttgccggggt ggcgctctcc caggcgacac ggctggccgt cgaatccgac cccgcgagtg
     3061 ccaatgacag ccgcgtcacc cagtcacaac tcctcaaccg gttgctgctg gaggatacct
     3121 accccgaatt catggttaag agcgattgcc cgttgcaggt agccctggcg gaggacataa
     3181 ccaagctcat gaagggtccc tcccttttcg aggcgcagcg ctttgccggg gttcccttgt
     3241 cggacgagac gaagcaattg ctggcgcagg caccccaggg cggcgagctg gtccgcctga
     3301 accgctgcct gctggaagat gccttccccg ccgacctatc caagaaccgg gtcggcgttc
     3361 ccggcagcgt caacccctgg tacgtcatga tcttcctctt tgtcgtcctc ctctcggtgg
     3421 gcgaagcctt ctattcaccg cggctctatg aatacacggc ggcgatcgct ccgaaggggc
     3481 aggaagcctc ctacatggcc atgtcttcgc tgccgttctt cctggccaag ctgggcgtcg
     3541 cccccttctc cggcgtgctg ctggcccatt tctgtcccga cataggaccg cgccatccgg
     3601 ggacgctgtg gctgctcatc ggcttgagca caatgattgc gccggtgggg ttgttcgtgt
     3661 tccagcgttt catccgcgtc cgcgaagcgg gtcgtgacga gtgaaggccc cgcggggttt
     3721 gaattagcct aatgagccta acaacccaga ttggctccgc cgcccctacg gcgacagaca
     3781 gcggatggag cgggggcctt gatgaggcgg cgttctacaa caccgtgctt ggggtgggaa
     3841 ccatctggaa tcacctcctc gtcatggtcg gcccgccgac gctgagctac tcgctcgccg
     3901 gcacccaact cactctctct tggccaacgg acgtgaccgg gtttaccttg gagtatgctg
     3961 agagcctgcc tgcgacctcg tggacgccgg tgagtggcgt ggtcaataac caggtcgccg
     4021 ttgacgcgtc cagcggcaag cggttcttta gacttagaaa gtgaacacct ccggaagcac
     4081 cagtatcggt ttgtcccggc gggaagacac tccccgccgg gccttcaccc tcatcgaatt
     4141 gctcgtggtg attgcgatca tcgccattct cgcggcgttg ctgctgccgg ccctggccaa
     4201 agccaaggag aaggcctacc gcatcgcgtg cacgaacaac ctgaagcaga tcgggctgtt
     4261 cctgcagttc tacaccgatg acaaccggga tgtctttccg gcccaccgga atcaaaacga
     4321 gaacgataac cccaccaccg ccctcaccaa ctggtggggg acggccatca tcggctacgc
     4381 gcgcaaccaa tcgaacgtgt tctgttgtcc agccatcaaa gggaagcgcc tggataacgg
     4441 cgtggcttgg agttggtcct tcgactgcca caaggtgggg tacggaatca actccttctt
     4501 tgactgcctt tggccctacg ggggagccag cgtctcaatc agcggcatca cgttcgactc
     4561 gaagccctgg ttcaaacgca gcgcggtgaa gagtccggcg gacaacctga tcatcggcga
     4621 cggcatgccc aaaagcgatg gcacgtatag ttcgagccta tggtggccca acgcctgtat
     4681 ggataaggtg aaaagtgcca gccagggttt cgaaggcatt gacaatctcc ggcatcgaaa
     4741 caccggggta gccgttttta ccgactgcca cgctgaggcc cgcaaagacg cgatgattaa
     4801 cccgcccgtt gacccgggcg ccggagatcc taaggggctt atgaattcga agtactggga
     4861 tccgctgaaa cgagcaggcg accgctgaag gcgagataag ccggcggcag aaggaccatg
     4921 aagcaagcca ccagcagcaa gccggcctgg tagaagacag aaacccggca ccgcggcatg
     4981 gcgccctcca gtgcggcctt caactcagcc gcggtggaaa ggccagtctt ccttggcggc
     5041 ctgcttgaac tcgtccagga agtccaacgg cagggccagc ggataattca cgctcttcat
     5101 ggtggaaaca tacattggcg ggccttcaaa tcaaaccctt gttgcgctga cccgtttacg
     5161 cctgtgcatg caaagccacg ccggtgactt tgtgtcaata gtgctcggtt atgttgcagg
     5221 cggcgggata ggcatgcggg gcggggcggt ttgccacagt agtgcaatct ccgccggggt
     5281 cagggcgcag gcctggttga cgaggtcgct gagcgtgcgc tccaagctca aggtttcggc
     5341 agcgagggcg tgggcgggtt cgatggtgcg ggtctgcaat cccaacggga ttgagtcctc
     5401 cagcccaggg ttgcgagctg cgagctaccc tgggaaaatg ggtccgggag aggccaaccc
     5461 caacggggtt gcgaccaccg cgcttcgtct caggccgcaa ccccgttggg gttgacgccc
     5521 aatatcgtgg ccatcccagg gtagctcgcg gctcgcaacc ctgggctttg tggcggaatc
     5581 cctttgggat tcaacgcggg tgatcacggc ctgggtgctg gaatgggcag gcggggcggg
     5641 gcggtttgcc acgtgagggc gatctcggct ggggttaggg cgtaggcttg gttgacg
//
LOCUS       DATCGS010000277         4966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_74363, whole genome shotgun sequence.
ACCESSION   DATCGS010000277 DATCGS010000000
VERSION     DATCGS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74363"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VIL39_05685"
     CDS             complement(<1..476)
                     /locus_tag="VIL39_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5480952.1"
                     /translation="MATIKPFAALRPDPGLASRICELPYDVMSSEEARQIAASNPLSF
                     LHVSKPEIDLPPATDVYAPEVYARGRENFQRLIAQGALRQDPQAYFYLYRQVMGAHRQ
                     TGLVAAASCEDYLKGIIRKHELARTEKEDDRVRHIEALDSQTGPVFLVYRANQAMD"
     gene            complement(798..1976)
                     /locus_tag="VIL39_05690"
     CDS             complement(798..1976)
                     /locus_tag="VIL39_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5480953.1"
                     /translation="MLLIPKTMQAVVYRGANDLRLETVPVPRIGPGELLVRVAVCGVC
                     PTDIKKIQYGTIPPPRIFGHETAGTIVRVGSRLQQFRVGERVALHHHVPCLECHACRH
                     HAFAQCAQYKRTGITAGFEPAGGGYAEYVRVMPFVLPGVVRIPARNSFLEGALLEPVN
                     TVLKGVKRLALLPGDTVLVAGQGPIGLMFTRLLALHGMRVLATDLLPARLKLARAFGA
                     AAVYCPESKVQSPKPKVEGNPKPEARSPKSSITHHASRITHPAANPQLSTLVHQLTKG
                     RGLDAAVIAVPSDAVVREAQALIRGAGQVLLFAHTRRGAETSIDPATVCMDEKDLIGS
                     YSSDYLLQKEVARLVFSRKLDAPRLITHQFPLAQTAAAIELASHPTAESLKVVVVQDE
                     "
     gene            2115..2642
                     /locus_tag="VIL39_05695"
     CDS             2115..2642
                     /locus_tag="VIL39_05695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5480954.1"
                     /translation="MDRKIEFETPWFQVVAKSVAGESAPYYTLRMQDYACVVAFTNRD
                     ELVLVRQYRPAVERHTLELPSGHVEPKEPPAESARRELAEECGLDAPRLELLGTLISD
                     TGRNENQLWCYFAARVLPLGADYVPEPGVERILVPRAKVPELIARAEFDHALHLAALM
                     LAVVKHGPRVFALPD"
     gene            complement(2738..3223)
                     /locus_tag="VIL39_05700"
     CDS             complement(2738..3223)
                     /locus_tag="VIL39_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5480955.1"
                     /translation="MQLKFDWLIVLENLHRGIMVTDVTMDAPVGPRIIYVNRAWMKIT
                     GYDRADIGGKTPRILQGKHTDRNLLRTLKLKLLNREVFHGQTWNYRKSGQPFMMNWYC
                     YAIYGDNGKPIYYVAEQEDATELETLRMKQRLLLNPLDPDANKFFAILAEYKAARKQA
                     R"
     gene            complement(3317..3832)
                     /locus_tag="VIL39_05705"
     CDS             complement(3317..3832)
                     /locus_tag="VIL39_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480956.1"
                     /translation="MLNDILRTDIELATRLMEEKRPAEEIIAALVPRGIDRAKATQLV
                     DDLRSGKKVDIQATLPMELGLARRSRSRSGSRGARQDQPSHSSPAESHREPSRRTSAH
                     SHKKSAVPWLIPALIVVLVVVVGGILVFQRYRADADFPMERTTNAPAPKVNVGSTPAP
                     AKVATPGKNSK"
     gene            complement(3963..>4966)
                     /locus_tag="VIL39_05710"
     CDS             complement(3963..>4966)
                     /locus_tag="VIL39_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5480957.1"
                     /translation="GPPNAPDSLATNSPPPGMREGRSREPGGRPRRPFWLRGMDEKEF
                     QSLIAKRTLHGLVLTLSTEGLEAAATRDLWLRTIIVLLAALAALGSGLAWRNLAKSSE
                     LQIRLVRASEMNTHLKEMNLAAAGLAHETRNPLNIIRGLAQMISKQPDTAAEIQRKSR
                     DIIEEADKVAAQLNEFINYSRPREVRRTKLSLSSVVNEVVRTLNYDLEEKKISLQVKG
                     EQLSIEADEQLLRQALFNLLLNAIQAVEGGGEIQVVAQKRNAAEAVVEVRDNGPGVPL
                     ERRTEIFKPYFTTQEKGTGLGLAVVQQIVLAHGWEVECLANEPRGATFRISHLKLAG"
BASE COUNT          894 a         1461 c         1635 g          976 t
ORIGIN      
        1 ttgtccatcg cctggttggc gcgatagacg agaaagacgg gacccgtctg ggaatccagg
       61 gcctcgatgt gccggacgcg gtcatcttcc ttctcggtcc gggccagctc gtgcttgcgg
      121 atgatacctt tcaggtaatc ctcgcaactg gcggcggcca ccaagccggt ctggcggtgc
      181 gcgcccatga cctggcggta gagatagaaa tacgcttgcg ggtcctgccg gagcgcgccc
      241 tgggcgatga gacgctggaa gttctcccgg ccccgggcat agacctccgg cgcgtaaacg
      301 tccgtggcgg gcggcaagtc tatctccggc ttgctcacgt gcagaaagct cagcgggttg
      361 ctggcggcaa tctggcgcgc ctcttcggac gacattacgt cgtacggcag ctcgcaaatg
      421 cgcgaagcca gcccggggtc tggccgcagc gcggcaaaag gcttaatggt agccatgaat
      481 acggcagaag ctaccgtccg ttgcgccgaa acgcacgcag aaagacaatg gacagagacc
      541 aaagcgcgcg tccttgccac cccgcttccg cgacgctggc ctcgggcctc tggccggctg
      601 gcggcaaggg tgatgctggc ctgatgctcc cgtgatgctt cgaaggagtc tcgaaggggt
      661 tacgatgggg cttcgaagga gtttcgaagg aattcaagga aaatcagccc ctgttacggt
      721 cgcgcgaccg tctcagcaac gccaagccaa gctagctccg gctctgtcat tagacgcctg
      781 agcttcggcg cggttcctca ctcgtcctga accaccacga ctttcaggga ttcggcagtg
      841 ggatgggaag ccagttcgat ggcagcggcg gtttgcgcga gcgggaactg gtgcgtgata
      901 agccggggcg catcgagctt ccgcgagaag accaggcggg ccacttcctt ttgcagcaag
      961 taatccgacg agtagctgcc aatcaagtcc ttctcatcca tacagactgt ggctgggtca
     1021 atggacgttt cagcgccgcg ccgggtgtgg gcgaagagca acacctgacc cgcgccgcgg
     1081 attagcgcct gagcctcacg cacgacggcg tcggaaggca ccgcgatgac tgcggcgtca
     1141 agaccacggc ctttcgtaag ctgatggacg agagttgaga gttgagggtt agcggctgga
     1201 tgcgtgatgc gcgatgcgtg atgcgtgatg ctcgacttcg ggcttcgggc ttcgggtttt
     1261 ggatttcctt cgactttggg ctttgggctt tggactttgg actcaggaca atagaccgcg
     1321 gcggcgccga acgcgcgcgc cagcttcagc cgcgcgggga gcaggtcggt ggccagcacg
     1381 cgcattccgt gcaaggccag caggcgggtg aacatcaggc cgatcggtcc ctggccggcc
     1441 accagcaccg tgtcgcccgg caatagcgcg agccgtttga cgcctttgag aacggtgttg
     1501 accggttcga ggagcgcgcc ttcgagaaag gaattgcgcg cggggattct gaccacgccc
     1561 ggcaggacga agggcatgac ccggacgtat tcggcatagc cgccgcctgc cggctcgaag
     1621 cctgccgtga tgcccgtgcg cttgtactgc gcgcactgcg cgaaggcgtg atgccggcag
     1681 gcgtgacact cgaggcacgg cacgtgatga tgcagcgcca cccgttcgcc gacccggaat
     1741 tgctggaggc gtgagccgac acggacgatg gtgccggccg tttcgtggcc gaagatgcgc
     1801 ggcgggggaa tagtgccgta ttggatcttc ttgatgtcgg tcgggcagac gccgcagacg
     1861 gcgacccgaa caagcaattc gccgggcccg atgcgcggca cgggcacggt ttcgagccgc
     1921 agatcattcg ccccgcgata aaccactgcc tgcatggtct tgggaatcag aagcataggg
     1981 gcattgagcc atagatggca ctgatttcac agactaaaag cgtgatgcgt gatgcgtgat
     2041 gcttgcattg ccacctgctg agactcagac ttgtggtggt tgaaggttaa agagtgatat
     2101 ctatgacgca gatcgtggat cgaaagattg agtttgagac cccctggttc caggtggtgg
     2161 ccaagagtgt ggcgggcgaa agcgccccgt attacacatt gcgcatgcag gattatgcgt
     2221 gcgtggttgc ctttacgaac cgggatgagc tcgtcctggt gcggcaatac cgtccggcgg
     2281 tagagcggca caccctggaa ctgcccagtg gccatgtcga gccgaaggaa ccgccggcag
     2341 aatccgcccg gcgcgagctg gcggaagagt gcggcctcga tgccccgcgg ctggaactgc
     2401 tcggcacgct gatcagcgac accggccgca acgaaaacca gctctggtgc tacttcgcgg
     2461 ccagggtgtt gccgctgggg gcagactatg tgccggagcc cggggtggaa cgtatcctcg
     2521 tgccgcgggc gaaagtgcct gagctgatcg ccagggccga atttgaccat gccttgcacc
     2581 tggccgcgct catgctggcg gtggtgaaac acgggccgcg ggttttcgcc ttgcccgact
     2641 aattggccac ggagccagcg caggatcagt gtagctggtc tgctccgcaa ccgcgatggt
     2701 tttccccggg caacagagcc ccggacgcat ccgaacgcta tcgtgcctgt ttgcgggcgg
     2761 ccttgtactc ggccaggata gcgaagaact tattggcatc gggatccagc ggattgagca
     2821 gcagccgctg tttcatgcgg agagtttcca gctcggtggc gtcctcctgc tcggcgacgt
     2881 agtagattgg tttgccgtta tcgccgtaga tcgcgtagca ataccagttc atcatgaacg
     2941 gctggccgct cttgcggtag ttccaggtct ggccgtgaaa gacctcgcga ttcaggagct
     3001 taagcttgag tgtccgcagc aggttccggt cggtgtgttt gccttgcagg atgcgcgggg
     3061 tcttgccgcc gatatcggcc cggtcatacc cggtgatctt catccaggcg cggttgacat
     3121 agatgatccg cggaccgacg ggggcgtcca tggtcacatc ggtgaccatg atcccgcggt
     3181 gcaggttttc cagaactatc agccaatcaa atttcaattg catagtgtcc gttgctcttg
     3241 gttgccctgg tgcgggcaac ctttccgtca atcatagcgc acattgccat ttacgccaaa
     3301 tctcagggcg gcaggatcac ttggaattct tgcccggcgt ggcaaccttt gccggcgccg
     3361 gcgtggagcc gacgttcacc ttcggcgccg gagcgttggt tgtccgctcc atagggaagt
     3421 cggcgtccgc acgatagcgc tggaaaacga gaatgccgcc caccacgacc accaagacaa
     3481 cgatgagtgc cggaatgagc cagggaaccg cggatttctt gtggctatgg gccgatgtgc
     3541 gccgggacgg ctcgcggtgg gattcggccg gcgaggaatg cgagggttga tcctgcctgg
     3601 ccccccgtga accgctcctc gaccgtgacc gccgcgccag gccgagttcc atcggaaggg
     3661 tggcctggat gtcaaccttc ttgccactgc gcaggtcatc caccagttgg gtcgctttgg
     3721 cccggtcgat gccccgaggg actagcgccg cgatgatctc ctcagcgggg cgcttttctt
     3781 ccatcaaccg ggtggcgagt tcgatatcgg tccgcaaaat gtcgtttaac atacctgtgg
     3841 caaacttctc tacaggcaaa cgggagcatt tacaagccgg caattgtggc gatcaggaat
     3901 tccgtatgct ggtcccccta aaccagatgg ccggagtagg gatgtccggg tcgcgccttc
     3961 gcctacccgg cgagtttcaa gtggctgatg cggaaggtag cgccccgggg ctcgttggcg
     4021 aggcattcga cctcccagcc gtgcgcgagc acgatttgct ggaccaccgc caggcccaag
     4081 ccggtgccct tctcctgagt cgtgaagtaa ggtttgaaga tttcggtgcg gcgctccagg
     4141 ggaacgcccg ggccgttgtc gcggacttcg accaccgctt cggcggcatt gcgcttttgg
     4201 gcgacgacct ggatttcgcc gccgccctcg acggcttgga tggcgttgag cagcaaattg
     4261 aagagcgcct gccggagcaa ttgctcatcc gcctcgatgc tgagctgctc gcccttgact
     4321 tgcaggctga tcttcttctc ctccaggtcg tagttcaagg tccgaacgac ttcattgacc
     4381 accgagctca gggagagctt ggtgcggcgg acttcgcgcg ggcgggagta gttgatgaat
     4441 tcattcaact gcgcggccac cttgtcggcc tcctcaataa tgtcgcgcga cttcctctga
     4501 atctccgccg cggtgtcggg ttgcttggaa atcatctggg ccaggccgcg gatgatattg
     4561 agcgggttcc gggtttcgtg ggccaggccc gcggcagcga ggttcatctc cttgagatgg
     4621 gtgttcattt cgctggcccg caccaggcgg atttgcagtt cggaggactt tgccaggttt
     4681 cgccacgcca agccggaacc gagagcggcg agcgccgcca acaggacgat gatcgtccgc
     4741 aaccacaaat cacgagtggc tgcggcctcc agcccctcgg tggacagggt gaggaccagc
     4801 ccgtgcaggg ttcgcttggc tatcaaggat tggaattcct tctcgtccat gccccgcagc
     4861 cagaagggac gccggggccg gcccccgggc tcccgtgacc gaccctcccg cataccgggc
     4921 gggggactgt tggtggccag actgtcggga gcattcggag gtccgg
//
LOCUS       DATCGS010000278         3341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_569378, whole genome shotgun sequence.
ACCESSION   DATCGS010000278 DATCGS010000000
VERSION     DATCGS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569378"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..378
                     /locus_tag="VIL39_05715"
     CDS             175..378
                     /locus_tag="VIL39_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin RelB"
                     /protein_id="HEY5480958.1"
                     /translation="MSTQEIAEAVMELPEKERLELARRIVASIVAEQDADGAISRAVQ
                     GIEDVVTGKVRGLSETEFRDALL"
     gene            747..1763
                     /locus_tag="VIL39_05720"
     CDS             747..1763
                     /locus_tag="VIL39_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5480959.1"
                     /translation="MTIPTEQPRTAEEILALGRSYQGAAVLAAAADLDLFSALSAAPL
                     AAAELAHTLQCDLRALTILLDALVALRLLEKSGSHYTLPAGLDAFLTPDGAQSILAMA
                     QHQGHCLRNWAQLGRVVKTGRPAERMPGVRGEAGDQEAFIGAMHNISAPNADQVIRAV
                     QPLQFRHLLDIGGASGTWTIAFLRACPSAQATIFDLPHVLPMARRRLADAGFDQRVKL
                     VAGDFMTDALPPGADLAWVSAIVHQNSRAQNRALFTAVFQALTPGGRIAIRDILMEED
                     RTRPVAGALFAVNMLVATEGGGTFTLAELREDLMAAGFAEVALARQDEAMNAIVVAHK
                     PGAA"
     gene            complement(1779..2504)
                     /locus_tag="VIL39_05725"
     CDS             complement(1779..2504)
                     /locus_tag="VIL39_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480960.1"
                     /translation="MLGLLALPSNRTARAWWIWAPLVVLALLGAGLGAFADAANNEGL
                     SFSAQAAGAAAFGLAAVWLVGAGLARRCRTLSIVLMALAFAAVSLLAFAVSPMWEQLW
                     DLRRYEPAIPLYLLLFWIVGGLVFAGGLNLTGWMCRKRFSCLRVLLRLPFWLWVMWFV
                     AGSLLCCVMTFGFNNSFEWTGILVEPLVLALVSFGLILPYLILSFACSFYRERLKDLL
                     RLPATDSSPPPRASPVATPESPR"
     gene            complement(2504..3169)
                     /locus_tag="VIL39_05730"
     CDS             complement(2504..3169)
                     /locus_tag="VIL39_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480961.1"
                     /translation="MFAGIALIWDQPKHKLYLDLNRNLDLTDDPTGAFSSTGKEFQQV
                     LTNVTLPLKTATGLHPAILDLHLFTDAQGRWAQVRLHSRSLWQAKVAPGGEEWQVAVV
                     DNLFGPEGPVVAKFLLLRPWAVRTNRVSFYDPTFGIIPFPSHLFWLGRPFQLGRRFET
                     DGESPVCKLEFTPQQPPLTELKLSGEFVCMQTWEAWERNRVRRFDSFGRCPVFSRCCS
                     RGW"
BASE COUNT          702 a         1038 c         1021 g          580 t
ORIGIN      
        1 agcgaaccgg caccgccgcc cagggctggc gccgcgcggc cagtaagcta tgggaaaagc
       61 cgcaagtcgc gctcgtcggg gcgccggcag gttccgttct gatcaggctc acaacaccgc
      121 tcaacgccga gacaccgctt gatcgggtta aggccatcga ttaaggtttg tgttatgagc
      181 acgcaggaga ttgctgaagc ggtcatggaa ctgcctgaga aggagcgctt ggagctagcg
      241 cggcgcattg tcgcgagcat cgtcgcagag caggacgccg acggggcgat tagccgcgcg
      301 gtccagggga tcgaggatgt ggttacgggc aaggtgcgcg gtctgagtga gacggagttt
      361 cgcgacgctc ttctatgagg gtttcctatc accccgattt tccgaaggac ataaagaggt
      421 ttgaagccca atacctggaa gtgtccgaac ggcttgcttt gcgatttcgc gcagtagtgg
      481 atgaggcgat caagcagata aaggcctcgc cgacttctgc gggtcacttc gtcaacacgg
      541 ggtcccggat cgtaaaggaa gtccgaagat gcaatctctc ttcgtttccc attttcattc
      601 tgtatggtgt ttctgcagat atgctggtct ttcggtcgct catcgccagt gcctcagacc
      661 ccttgacctg gctaacgcgt ttccccgagg caaccgctgg agaaccgcca gatggcagaa
      721 ccacgtaaca gaggcaaggc cacattatga cgattccaac cgagcaaccg cggacggctg
      781 aagaaattct ggccttggga cgcagctatc agggcgccgc ggtcctggcc gccgcggcgg
      841 acctggacct ctttagcgca ttgtctgccg cgccgctggc ggctgcggaa ctcgcccaca
      901 ctctccagtg cgatctgcgc gccctgacta ttctcctgga tgccctcgtc gcgctgcgac
      961 tgctcgagaa gtctggcagc cattacaccc tgccggccgg tctggatgct tttctgacgc
     1021 cagacggagc gcaaagcatc ctggccatgg cccagcatca agggcattgc ctccgcaact
     1081 gggcgcaatt gggccgcgtc gtcaaaaccg gccggcccgc cgaacgcatg ccaggcgtcc
     1141 gcggcgaggc gggcgatcag gaggccttca ttggcgcgat gcataacatc tcggcaccca
     1201 atgccgacca ggtgatccgg gcagtgcaac ccctgcaatt ccgacacctg ctggacattg
     1261 gcggcgcttc gggcacctgg accatcgcct ttctgcgcgc ctgtccctcg gcccaggcca
     1321 cgatcttcga cctgccgcat gttctcccga tggcgcggcg gcgtctggcc gatgctggct
     1381 tcgatcagcg cgtcaagctc gttgccggcg acttcatgac cgatgcgttg ccgccgggcg
     1441 cggaccttgc gtgggtgagc gccattgtgc accagaattc ccgcgcccag aaccgcgcgt
     1501 tgttcactgc ggtatttcaa gcgctgacgc cgggcggacg catcgctatc cgcgacattc
     1561 tgatggagga agaccgcacg cgccctgtgg cgggcgcgtt gtttgccgtg aacatgctcg
     1621 tagccacgga aggcggcggc acgttcacct tggctgaatt gcgggaggac ttgatggccg
     1681 cgggctttgc tgaggttgcc ctggcgcgcc aggacgaagc gatgaatgcc atcgtcgtcg
     1741 cgcataaacc tggcgcagcc tgagccaggg agggggcttc agcgcgggct ttccggcgtg
     1801 gctacgggtg aagcacgagg aggaggcgag gaatccgttg ctggcaatcg caacaggtcc
     1861 ttgagccgct cgcggtagaa cgaacaggcg aacgagagga tcaggtaagg cagaatcaac
     1921 ccaaagctca cgagagcgag aacgaggggc tccaccagaa tccccgtcca ctcaaagctg
     1981 ttgttgaaac caaacgtcat cacgcagcag agcaggctgc cggccacgaa ccacatcacc
     2041 cagagccaga acgggagccg caataagacc cggaggcagc taaaccgttt gcggcacatc
     2101 cagcctgtca gattcagccc gccggcaaac accaggccgc cgactatcca gaacagcaac
     2161 agatacaggg gtatcgccgg ctcatatcgt ctcaggtccc atagctgctc ccacatcggg
     2221 ctgaccgcga acgccagcag gctgactgcg gcgaaggcca gcgccatgag cactatgctg
     2281 agcgtccggc atcgtcgcgc caggcctgct cccaccagcc acaccgccgc cagcccgaag
     2341 gccgcagcgc ccgccgcctg ggccgaaaag ctgaggcctt cattattggc agcatcagca
     2401 aacgctccca aaccagcgcc gagcagagcc aggaccacca gcggcgccca aatccaccat
     2461 gcccgcgcag tgcggttcga cggcaacgcg agcaggccaa gcactaccaa ccacgggagc
     2521 agcagcggga gaagactggg cagcgtccaa acgaatcgaa ccggcggacc ctgtttcgtt
     2581 cccaggcctc ccaggtctgc atacagacga attcgccgga gagcttgagt tccgtcagtg
     2641 gcggttgctg cggcgtgaat tccagcttgc agacggggga ttcgccgtct gtctcaaagc
     2701 gacgccccaa ttggaacggc cggcccagcc agaaaaggtg gctgggaaag gggatgatgc
     2761 cgaaggtggg atcatagaac gacacacggt tcgtccgcac cgcccacggg cgcaatagca
     2821 ggaactttgc gacgacgggg ccctccgggc caaaaaggtt atccacgacg gccacctgcc
     2881 attcttcacc tccaggcgcc accttggctt gccacagaga acgcgaatgc aaccgcacct
     2941 gagcccagcg cccctgcgcg tctgtgaaca gatgcaaatc caatatcgca gggtggaggc
     3001 ccgtcgccgt cttcaggggc aaagtgacat tagtaagaac ctgctggaac tccttgccgg
     3061 tcgagctgaa ggctccggtt ggatcatcgg tcaaatccag gttccggtta aggtcaaggt
     3121 agagtttgtg cttcggctgg tcccagatca gggcaatgcc agcaaacata ctgcaataag
     3181 cagcacgcgg acaggccgca aaatcatggg tggaatcgta agcatgggtg ccagacaagt
     3241 caagactggc acctaacgca acccttggtc gccagggcca attcatgata ctcactaacc
     3301 cgttgtaaac tcaggggtta gcgctttctt tggacgcttc c
//
LOCUS       DATCGS010000279         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_220166, whole genome shotgun sequence.
ACCESSION   DATCGS010000279 DATCGS010000000
VERSION     DATCGS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220166"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VIL39_05735"
     CDS             complement(<1..491)
                     /locus_tag="VIL39_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5480962.1"
                     /translation="MPNTTVIGIDLGGTKLAGALFTPKGRILSKNIVPLAGRQGRAVG
                     ELIRDELALLLAAARVRKLKLTAIGVSVPGISHAKTGKVWAPNIPGWKDYPLRRELLA
                     ALPDKRIRVVMDSDRAAYILGETWRGVAKGCHNAIFLAVGTGIGAGILIDGRVLRGAH
                     DIAG"
     gene            complement(503..1681)
                     /gene="hemW"
                     /locus_tag="VIL39_05740"
     CDS             complement(503..1681)
                     /gene="hemW"
                     /locus_tag="VIL39_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5480963.1"
                     /translation="MAEFSAEPIASLYVHVPFCVRKCLYCAFYSEPSSGELIDRYVGA
                     LTHELERVTADLKPRTIFFGGGTPSLLNLRQWERIFRAMERIPKSGVMEWTVECNPAT
                     VSLEKAKLLRAGGVNRVSLGVQSFDDALLDRLGRVHSRQMVFESFDILRQAGFDNVNL
                     DLMFAIPGQTLEVWRETLAEAAAMGSEHLSTYEVIYEEDTPLYDQLQAGKVDVDEDLA
                     CAMYEELVERAANAGFHQYEVANFARDTTLNPQPSTLDIPSRCCFHNVNYWRGGSFYG
                     LGPSATTYVRGVRTKNGSNTHLYCEQLEQGRRSLESREELLPLARAGETAAFGLRMVA
                     GWPFEQFRQVTGYDLRREWAEDMNQLVQQGRGRILPDRFQLTREGLRFADSAAQLFLR
                     "
     gene            complement(1837..>2151)
                     /locus_tag="VIL39_05745"
     CDS             complement(1837..>2151)
                     /locus_tag="VIL39_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480964.1"
                     /translation="NRLRDLNYRVQTIADAERLVEYAEEAKAMLVLADLESRRNNVCA
                     ALARLKEHPATKHLPVIAFSGESAVELQAAARAAGATLVVTEVAILNHLPQLLEQALQ
                     IE"
BASE COUNT          401 a          649 c          704 g          397 t
ORIGIN      
        1 ccggcgatgt cgtgtgcgcc gcgcagaacc cggccgtcga tcaagatgcc cgcgccgata
       61 cccgtgccga cggccaggaa aatggcgttg tggcagccct tggcgacgcc gcgccacgtc
      121 tcgcccagga tgtaggcggc ccgatcactg tccatgacga cccggatgcg cttgtccggg
      181 agcgcggcca ggagttcccg gcgcagcgga taatccttcc agccgggaat gttcggcgcc
      241 cagaccttac ccgtcttcgc atgactgatg ccggggacgg agaccccgat ggcggtgagc
      301 ttgagttttc tgacgcgggc agcggccagg agcagggcga gttcgtcgcg gatcagttcg
      361 ccgacggcac ggccctgccg gccggcgaga ggaacgatgt tcttgctgag aatcctgcct
      421 ttgggggtga aaagcgcgcc ggcaagcttg gtgccgccca ggtcgattcc gatgacggtc
      481 gtgttgggca tgagttagaa tatcagcgca ggaagagctg ggcggcggag tcggcgaagc
      541 gcagaccttc ccgggtgagt tggaagcggt cgggcaggat gcgccccctg ccctgctgca
      601 ccaactggtt catgtcctcc gcccactcgc ggcgcaaatc gtaaccggtt acctgccgga
      661 actgctcgaa aggccagcct gcgaccatcc gcagaccaaa ggcggccgtt tccccggcgc
      721 gagccagcgg gagcagttct tcgcgcgatt ccagcgaccg ccggccttgc tcgagctgct
      781 cgcagtagag gtgcgtattg gaaccgttct tcgtgcgcac gccgcggacg taggtggttg
      841 cgctcgggcc cagcccgtaa aaggagcctc cccgccagta attgacgttg tgaaagcagc
      901 agcggctggg aatgtcgagg gttgagggtt gagggttgag ggtggtgtcg cgggcgaagt
      961 tggcgacttc gtattggtgg aagccggcgt tcgcggcgcg ctcgaccagt tcttcataca
     1021 tggcgcaggc caaatcctca tccacatcaa ccttgccagc ctggagttgg tcatagaggg
     1081 gcgtgtcctc ctcgtagatc acttcgtagg tggagagatg ctcgctaccc atcgcggcag
     1141 cctcggccag agtctcgcgc cagacttcga gggtttggcc agggatggcg aacatcaagt
     1201 cgaggttcac attgtcgaag cccgcctggc gcaggatgtc gaatgactcg aagaccatct
     1261 gacggctgtg aacacgtccg agacgatcga gcagggcatc atcgaacgat tgcacgccca
     1321 gggaaacacg gttcacgccg ccggctcgga gcagtttcgc cttctcgagc gacacggtag
     1381 ccgggttgca ttctacggtc cactccatga ccccggattt ggggattcgc tccatcgcac
     1441 gaaagattcg ctcccattgc cgcaggttca ggagcgaggg tgtgccgccg ccaaagaaga
     1501 tcgtccgcgg cttcaggtca gccgtaacac gttccagctc atgggtgagg gcccccacat
     1561 agcggtcaat cagctcaccg ctggaaggtt cggagtaaaa ggcgcaatag agacacttgc
     1621 gcacgcagaa gggcacgtgg acatagagac ttgctatcgg ctcggcagag aactcggcca
     1681 tagccgacag tagccgcccc ccggacggct ggccaatcga aaagcctgcc acccaaacgc
     1741 ggggcggcgg cggaaatacc aacgaacggg ttgcaacgcg cccgcaggct caagcccttg
     1801 tttccgggca tttgttcttc ggtaagattg gagcgcttat tcgatttgca gagcttgctc
     1861 gagtaactgg ggcaggtggt tcagaattgc cacttcggtg acaaccaggg tggcgccggc
     1921 ggcccgggcg gcagcctgca actccaccgc gctttcgccg ctgaaggcaa tgaccggcag
     1981 gtgtttggtg gcgggatgtt ctttcaggcg cgccaaggcg gcacagacgt tgtttcgcct
     2041 ggattccaga tcggccagca ccagcatggc tttggcctct tccgcgtatt ccaccagtct
     2101 ctccgcgtcg gcaatcgtct ggacccgata attcaggtcc cgcagccggt t
//
LOCUS       DATCGS010000280         5136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_420895, whole genome shotgun sequence.
ACCESSION   DATCGS010000280 DATCGS010000000
VERSION     DATCGS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420895"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /gene="secD"
                     /locus_tag="VIL39_05750"
     CDS             <1..1920
                     /gene="secD"
                     /locus_tag="VIL39_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5480965.1"
                     /translation="FLEFRLVSPTSDQDIKEGIVQPGYEILKRKEKTRRGQERIEEVE
                     VRKRAEMDGSSIKSAMVVRGNLGEPEIHFTLDAKGTERFGEITRENIHQRLAIILDKE
                     LYSAPVIQSAIETGSGQITGQFDNKEAFELANVLENPLRAPLKIIQSREVDPTLGKDS
                     IRSGINAAIYGTLAVSVFMLVYYLAAGMVANIALIANIIILLGVMCSIGTTLTLPGIA
                     GIVLTVGMAVDANVLIYERIREESAKGKSLRGAIAAGYARAFTVIFDSHVTTLISSII
                     LMYMGTGSIKGFGVALTIGVAASLFTALVVTRLIFDFLVDRGWLKSLPMFHLIRATKI
                     DFMKLAKPAFVTSWILIAIGLGYGWHRGKAAFGVDFLGGDTTTFAFEQKVPEDVLRSA
                     LAKAGVKDPLIQYQKDVSSRRETLRVDSTTGTGDKVKGTLSEIPAAKFRLLGQDQIGA
                     TVGKEIQKTAAIAALLSLFGILIYVAVRYEFPFAVGAVLAIVHDVPMTIGWYCLSGRE
                     FNATTVAAILTIIGFSTNDTIVIFDRIREDLKLGVRGTFREVINQAINQTLSRTIITS
                     GTVFLATLSLYIFGGGSINDFAFTFLVGIITGTYSSIYIASTLVLWWHKGQRPTLGSG
                     AQVAVVEGAAATVRK"
     gene            1942..2928
                     /locus_tag="VIL39_05755"
     CDS             1942..2928
                     /locus_tag="VIL39_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5480966.1"
                     /translation="MLALVEITPWHWVGFVVCVLIFLALDLGVFHRHAHTVKFKEALV
                     WSIIWFSLAMLFALALKPLRGKQEALQFVTGYLIELSLSMDNVFVIALIFAYFRVPRE
                     HQHRVLYWGILGALIMRGLMIGLGLALIALLDWILYVFGAFLLYSGFRMLFVQTEVHP
                     ENNRVVRWARKLYPVTPELHGQRFIVPWEGRMAITPLALVLLMVETTDLLFALDSIPA
                     VFAVTRKPFIVFTSNVFAILGLRSLYFLLAGAIGYFRYLKYGLPAVLVFMGVEMLLDP
                     HNRQPPRWFQVEIPTSVSLVVVAGILLLAIVLSILAARHERKQKTAKPLKPD"
     gene            2977..4761
                     /locus_tag="VIL39_05760"
     CDS             2977..4761
                     /locus_tag="VIL39_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY5480967.1"
                     /translation="MKFRWSLAAPQPLLAAQLASALSIPPLLAQCLLNRGLCEPASIA
                     GFLQPRLKQLADPFLLPNMAAAVERLLQARQRNESLVIFGDYDVDGVTSTALLLEVLR
                     PLGWKVHAYLPHRMDEGYGLSQDGVENCLAKFPTTLLLAVDCGSNAVASIAYLRQRRV
                     DVIVLDHHQVASPAPAAVALVNPQLGVPSPISSNELTPSCNPCNPINSCNSPPPFTEL
                     CSAGLAFKLAHALVKRGRETGLPGAADFDLRPLLDLVALGTIADVVPLIAENRILVTA
                     GLERLNTTRRPGLVALKRVAQSPALLTTYEVGFQLAPRLNAAGRLETAEEALRLVLAA
                     DLAEALPIAQILDSHNRERQKIERGIADEVISALRTRFHTETDFVIVEGQLPWHIGVV
                     GIVAARVLQQFYRPTIILGGDGDLFRGSGRSIAGFDLAAALRQCDDLLVRHGGHAMAA
                     GVTLQAANLAPFRARLNDIARRALQPDALQAPLRLDAEVTLDTITEECLVSLDQLRPT
                     GLGNPSVQFFARNLTQQRSPLRMGAEKQHLKLWVTDGATTREAVWWGAGNEPLPAPRF
                     DLAFAPQINEYNGRRTVQLKILDWRPAE"
BASE COUNT          895 a         1762 c         1420 g         1059 t
ORIGIN      
        1 tttctggaat tccgcctggt aagcccaacc agcgatcagg atatcaaaga ggggatcgtc
       61 cagccgggct acgaaatcct caagcgcaag gagaagacgc gcagaggtca ggaacgtata
      121 gaagaggtcg aggtcaggaa acgggctgaa atggacggca gcagcatcaa gagcgccatg
      181 gtcgtgcgcg ggaatctggg cgagcccgaa attcacttca cccttgatgc caaaggaacc
      241 gagcgcttcg gtgaaatcac ccgtgagaat atccaccagc gcctggctat catcctggat
      301 aaggagctct attcggcgcc ggtcatccaa tcggctatcg aaacgggcag cggccagatc
      361 acgggccagt tcgacaacaa ggaggccttc gagttggcca acgtcctgga gaatccgctc
      421 cgcgcgcctc tgaagataat ccagtcccgg gaagtggacc cgacgctggg caaagactcc
      481 atccgtagcg gcatcaacgc cgccatctat gggacgctgg ccgtctcggt cttcatgctc
      541 gtgtattacc tcgccgccgg catggtggcc aatatcgcgc tcattgccaa tatcatcatt
      601 ctgttgggcg tcatgtgctc gatcggcacg accctgaccc tgccgggcat tgcgggcatc
      661 gtgctgacgg tcggcatggc cgtggacgcc aacgtcctca tctacgagcg cattcgcgaa
      721 gagtcggcga aaggcaagtc tctgcggggt gccattgccg ccggttatgc cagggccttc
      781 accgtcattt tcgactcgca cgtcacgacg ctgatttcgt ccatcatcct gatgtacatg
      841 ggcaccggtt ctatcaaggg cttcggcgtg gccctcacca ttggcgtggc cgccagcttg
      901 ttcaccgcgc tggtcgttac tcgcctgatc ttcgactttc tggtggaccg cggctggctc
      961 aagtcgctgc caatgtttca tcttatccgc gccaccaaga ttgatttcat gaagctggcc
     1021 aagcccgcct tcgtgacgtc ctggatcctg atcgccattg gcctgggtta cggctggcat
     1081 cgcggcaagg ccgcgttcgg cgtggatttc ctcgggggcg acaccacgac ctttgctttc
     1141 gaacagaaag ttcctgagga tgttctccga tctgctctcg ctaaagccgg ggttaaagac
     1201 ccgctcattc agtatcaaaa ggacgtgagc agccggcgtg agacactgcg cgtggactcc
     1261 accaccggga cgggtgacaa agtcaagggc actctgtccg agattcctgc cgcaaaattc
     1321 cgtctgctgg ggcaggatca gatcggggcc accgtgggca aggaaatcca gaagacagcc
     1381 gccattgccg cgttgctgtc gctctttggc atcttgatct atgtggcggt tcggtatgaa
     1441 ttcccgtttg ccgtgggcgc ggtgttagcc atcgtgcatg acgtaccgat gacgattggc
     1501 tggtactgcc tctccggccg cgagttcaat gccacgaccg tcgccgccat cctgacgatt
     1561 atcggcttct ccaccaacga caccatcgtt atttttgacc gtatccgcga ggacttgaag
     1621 ttgggcgtcc gcggcacatt ccgcgaagtg ataaaccagg cgatcaatca gaccctgagc
     1681 cgcaccatca ttacctccgg caccgtgttc ctggcaaccc tgtcgcttta catttttggc
     1741 ggcggttcga ttaacgactt tgccttcacc ttcctggttg gcatcattac cggcacctac
     1801 tccagcatct acatcgccag cacgctggtc ctgtggtggc ataaaggcca gcggccgacg
     1861 cttggctcag gagcgcaggt cgccgtggtc gaaggtgcgg cggctaccgt gcggaaatag
     1921 ccgcttgcgc ggtagcggta catgcttgcc ctggttgaaa tcaccccctg gcattgggtt
     1981 ggtttcgtag tctgcgtcct gatcttcctg gcgctcgacc tcggcgtgtt ccatcggcac
     2041 gcccacacgg tcaagttcaa ggaagcactc gtctggagca tcatctggtt ctccctggcc
     2101 atgctctttg ccctggccct caaacccctg cgcggaaagc aggaggccct ccaattcgtc
     2161 accggctatc tcatcgagct gtcactctcg atggacaacg tctttgtcat cgccctcatc
     2221 tttgcctatt tccgcgtccc gcgggaacac cagcatcgcg tcctctactg gggcattctc
     2281 ggcgctctca tcatgcgcgg cctgatgatc ggcctcggcc tggccctcat cgccctgttg
     2341 gattggatcc tctacgtctt tggcgccttc ctcctctata gcggcttccg gatgctcttc
     2401 gtccagacgg aagtgcatcc cgagaacaat cgcgtggtcc ggtgggcgcg aaaactctat
     2461 cccgtcacgc cggaacttca cggacagagg ttcatagttc cctgggaagg ccggatggcg
     2521 atcaccccgc tggcgctggt gctcttgatg gttgagacca ccgacttgct ctttgccttg
     2581 gattccatcc cggcagtctt cgcggtcacg cgcaaaccct tcatcgtgtt cacctcgaac
     2641 gtctttgcca tcctcggcct gcgatcgctc tatttcctcc tggccggcgc catcggctat
     2701 ttccgctact taaaatacgg cctgcccgcc gtgctggtgt tcatgggcgt ggagatgctg
     2761 ctcgacccgc acaaccggca gccgccgcgc tggtttcagg tcgagatccc cacctctgtc
     2821 tccttggtgg tggtcgccgg catcctgttg cttgccatcg ttctttccat cctcgccgcc
     2881 cggcacgaga ggaagcagaa gaccgctaaa cccctgaaac cagactgact cccccgtgcc
     2941 gttcgccgac cgcgacaccc tccctctgcc tctccaatga aatttcggtg gtcactcgca
     3001 gcgccccagc ccctcctcgc tgcgcagttg gcaagtgccc tgagcatccc gcccctgctc
     3061 gcccaatgcc tgctcaaccg cggcctgtgc gagccggcct caatcgcggg cttccttcag
     3121 ccccggctca agcaactggc cgatccgttc ctgctgccga acatggcggc cgccgtcgag
     3181 cgcctgctcc aggcccgcca acgcaacgaa tccctcgtca tctttggtga ttacgacgtg
     3241 gacggggtca cctccaccgc cctgctgctc gaagtgctcc gccccctcgg ctggaaggtc
     3301 catgcctacc tgccgcatcg tatggatgaa ggctacggcc tcagccagga cggcgtggag
     3361 aattgcctgg cgaagttccc caccaccctg cttctcgccg tggactgcgg ctcgaacgcg
     3421 gttgcatcca tcgcttacct gcgccagcga cgggttgacg ttatcgttct ggaccaccat
     3481 caggtcgcct cccccgcccc tgccgccgtc gccttggtca acccccaact cggcgtcccg
     3541 tctccaatat ccagtaacga actcaccccc tcatgtaacc catgtaaccc aatcaactca
     3601 tgtaactccc ccccgccttt caccgagctc tgttccgccg gcctcgcctt caagctcgcg
     3661 cacgccctcg tcaagcgcgg acgcgagacc ggcctcccgg gtgctgccga cttcgacctg
     3721 cgccccttgc ttgacctggt cgcccttggc accattgctg acgtcgtccc gctcatcgcc
     3781 gagaaccgca tcctggttac tgccggcttg gaacgtctga acaccacccg gcggcccggt
     3841 ctggtggcct tgaaaagagt cgctcaatcc cctgccctcc tcaccaccta cgaggtaggc
     3901 ttccagcttg cgccccgtct caacgccgcg ggtcgcctcg aaaccgctga agaagccctg
     3961 cgtctcgtgc tggcagccga cctggctgaa gccctgccca tcgcgcagat actggattcg
     4021 cataaccgtg agcgccagaa gatcgagcgc ggcattgcgg acgaggtcat cagcgcgctg
     4081 cgcacccgct tccacacgga gaccgacttc gtcatcgtcg agggccaatt gccctggcac
     4141 atcggcgtcg tcggaattgt ggcggcgcgt gtgctccagc agttctaccg ccccactatc
     4201 atcctcgggg gcgacggcga cctattccgc ggctcaggcc gcagcatcgc cggcttcgac
     4261 ctggccgccg ccttgcgcca gtgcgacgac ctgctggtcc gtcacggcgg ccatgccatg
     4321 gcagcgggag tcaccttgca agctgccaac ctggccccct tccgcgcgcg cctgaacgat
     4381 atcgcccgcc gtgccctcca acccgacgcc ctccaggcgc cgctgcgcct cgacgccgag
     4441 gttaccctgg acaccattac cgaggaatgc ctggtctccc ttgaccagtt aagaccgacg
     4501 ggcctgggca acccgtccgt ccagttcttt gcccgcaacc tcacgcaaca gcgttcacca
     4561 ctgcgcatgg gcgccgaaaa gcagcacctc aagctgtggg tcaccgacgg cgccaccacc
     4621 cgcgaggcgg tctggtgggg cgcgggcaac gagccgctcc ccgccccgcg cttcgacctc
     4681 gctttcgcgc cccaaatcaa cgaatacaac ggccgccgca ccgtgcagtt gaaaatcctc
     4741 gattggcgcc cggcggagta gtctccgcgc agctccctct ccccgcattt ccaggcccct
     4801 tgcgtcctta cctaatggcg ccttcgcaaa acagtcgaag ttgttcgcaa gctgcgaatg
     4861 cttttgtggc tgaactgcca cgcgaagcgt ttggagtgcg tccagcttgc tggcgctgtc
     4921 gttaaggagg gccgttccga aagcgggagc aagctccacg cactccaaac gcttcgcgca
     4981 attcgtttgc ggctgcgccg cgccgcggcc atcgcgccgc cctcgctgct gcggggcggc
     5041 tatgaggcag gcagaaccgg gaaacaaacc aggatcaagc gtggagatag ccaccgcatc
     5101 gggggatgca cgcgcctgct cttcgtattc tcttcg
//
LOCUS       DATCGS010000281         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_669312, whole genome shotgun sequence.
ACCESSION   DATCGS010000281 DATCGS010000000
VERSION     DATCGS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669312"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..904
                     /locus_tag="VIL39_05765"
     CDS             149..904
                     /locus_tag="VIL39_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5480968.1"
                     /translation="MTNPIIQDLLTLSHEIGREERGLAILGEGNTSARLSDETFAVKA
                     SGTCLGTLKAEDTVECRLDALLPLLDRDSLTDQQVDDALMAARVDQKAKKPSVEAVFH
                     AWLLSLPGIKFVGHGHPTTALQILCSPRARDFAEHRMFPDEIVCCNIASVLVPYTDPG
                     LKLAQTIRRQTDAFIQKHQRQPRVILMQNHGVITLGATWQSVLAAMLIAEKSARVFVG
                     AAALGGPVFFTEQNVQRIAGRPDEAYRQRALKM"
     gene            complement(969..1045)
                     /locus_tag="VIL39_05770"
     tRNA            complement(969..1045)
                     /locus_tag="VIL39_05770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1009..1011),aa:Pro,seq:tgg)
     gene            1262..2485
                     /locus_tag="VIL39_05775"
     CDS             1262..2485
                     /locus_tag="VIL39_05775"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5480969.1"
                     /translation="MSTQAPAAPQKINLRRPDVMAEVQAQVLSHYRSGLVERIRSTGH
                     LLSAGDLTIKLAKQFGFCYGVERAIDLAYAARKVFLNQPIYILGEIIHNPEVNDQIRA
                     MGIKFLSGKEKDADIADLKRDDIVIIPAFGVEVSMRRQLEAKGCRFVDTTCGDVMSVW
                     KRVRQYSKDQVTSIIHGKAWHEETKATSSQAMASGGGHYLVVFTLGETDYVCNYLLNG
                     GEKAAFLKKFKGAYSEGFDPDVHLQAIGVANQTTMLRGETEEVQRRLRRAMVQKYGED
                     EIDRHFRFFDTICGATQDRQDALEKLLQEPIDLLLVIGGYNSSNTSHLAEMGEAKLPT
                     YFIKNAAKMRSDKLILHYDQHKHEEVETANWLPSGKVTVGITAGASCPNNLIEDAIRQ
                     LFELRGISVQQLLEG"
     gene            2541..>3002
                     /gene="dgt"
                     /locus_tag="VIL39_05780"
     CDS             2541..>3002
                     /gene="dgt"
                     /locus_tag="VIL39_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEY5480970.1"
                     /translation="MPRTRQELEALERQSLAPYAQLSANTRGRKHREPAPEWRTEYQR
                     DRDRVIHSRAFRRLEYKTQVFLNGTGDHLRTRLTHTIEVAAISRNIARALKLNEDLAE
                     AIALAHDLGHSPFGHKGEMVLNRLMKDFGGFEHNRQSLRIVEALEQRYPRFS"
BASE COUNT          626 a          942 c          866 g          568 t
ORIGIN      
        1 caacgccgtc gtctttctcg cttccgacca atcctcctac atcaccggca tctccctgag
       61 cgtgaccggt ggccaggaga tgaactagtt tcccctgtaa cctttgtaac tcgtgtaacc
      121 ttgtaactaa tgtaactctg cgtttgccat gactaatccc atcatccaag acctgctgac
      181 cctttcccac gaaatcggcc gcgaagagcg cggcttggcc atcctcggcg aaggcaacac
      241 ctccgcgcgg ctcagcgacg agaccttcgc cgtcaaagcc agcggcacct gcctcggcac
      301 cttgaaagca gaggacaccg tcgagtgccg tctggacgcg ctgctgccct tgctggacag
      361 ggacagcctg acggaccagc aggttgatga cgccctgatg gctgcccgtg tggatcagaa
      421 ggccaagaaa ccttccgtcg aggcggtatt ccatgcgtgg ctgctctcgc tgcccggcat
      481 caaattcgtc ggccacggcc accccacgac cgcccttcag atcctttgtt ccccccgcgc
      541 ccgggatttt gcggagcacc ggatgtttcc cgacgagatc gtctgctgca acatcgcctc
      601 cgtgctggtg ccgtacaccg atcccggctt gaagctggcc cagaccattc gccgccagac
      661 cgacgcgttc atccaaaagc accagcgcca gccgcgggtc attctgatgc agaaccacgg
      721 cgtcatcacc ttgggcgcga cctggcaatc ggtcctcgcc gccatgctga tcgccgagaa
      781 atccgccagg gtctttgtgg gcgcagccgc gctcggtggc ccggtgtttt tcaccgagca
      841 gaatgtccag cgaatcgccg gccggccgga tgaagcctat cggcagcgag cgttgaaaat
      901 gtgagcccgc tcagcggcaa gagcgcaatc atcaccggcg ccagccgcgg catcggtagt
      961 cttttcagtg gtcggagcga caggatttga acctgcggcg tcttggtccc aaaccaagtg
     1021 ctctaccagg ctgagctacg ctccggcaac cgagtgtcta atatcccaca tccccagcgg
     1081 agcgcaatcc caaatctgac caaagcggac gaaagattag tcgaggaatg gagtgctggg
     1141 gagaggtgta gcaaaccagg cccattcgtc ccatcgaaca ttcagcaccc ttttggccta
     1201 tttgccccgc cgccgccttt ctgttggacg gtatgcgggt tcatgttaga gtgagagcgg
     1261 tatgtcaacg caagcgcctg ccgcgccgca gaaaatcaac ctccgccgtc ccgatgtcat
     1321 ggcggaggtc caggcccagg tcctctcgca ttatcgcagc ggcctggtcg agcgcatccg
     1381 gagcaccggg catctccttt cggcgggcga cctgaccatc aagctcgcca agcaattcgg
     1441 cttctgctat ggcgtcgagc gggccattga cctggcctat gccgcgcgca aagtcttcct
     1501 gaaccagccg atttatattc tcggcgaaat catccacaac ccggaagtga acgaccaaat
     1561 ccgcgccatg ggcatcaagt tcttgtctgg caaggagaag gacgccgaca tcgcggactt
     1621 gaagagggac gacattgtca tcattcccgc cttcggggtg gaagtctcga tgcggcggca
     1681 actggaagcc aaaggctgcc ggttcgtcga taccacttgt ggcgacgtga tgagcgtatg
     1741 gaagcgcgtg cggcagtact ccaaagacca ggttaccagc atcatccacg gcaaagcctg
     1801 gcacgaagag accaaggcga ccagttcgca ggccatggcc agcgggggtg gccattacct
     1861 cgtcgtgttc acgctgggcg aaacggacta cgtttgtaac tatctgctca acggcggcga
     1921 gaaggcagcc ttcctgaaga agttcaaggg cgcgtattcc gaaggttttg atcccgacgt
     1981 tcacctgcag gccatcgggg tagccaatca aaccacgatg ctccgcggcg agaccgaaga
     2041 agtgcagcgg cgcctgcgcc gggccatggt gcagaaatac ggcgaggacg aaatcgaccg
     2101 gcacttccgt ttcttcgaca ccatttgcgg ggcgacgcag gatcgccagg acgcgctgga
     2161 gaagctattg caggaaccga tcgacctgtt gctggtcatt ggcgggtaca attcctcgaa
     2221 cacctcgcac ctggcggaaa tgggcgaggc gaagctgcct acctacttta tcaagaacgc
     2281 cgccaagatg aggtcggata aattgattct ccactacgac cagcacaagc acgaggaagt
     2341 tgagaccgcc aactggctgc cttcgggcaa agtcacggtc gggatcaccg cgggcgcctc
     2401 ctgtccaaac aacctgattg aggatgccat ccgccagctc tttgagctgc ggggcatctc
     2461 agtgcaacaa ctgctggaag gataatgcgc ccctgtttcc ttcggccttg ggacctcggg
     2521 cttcggattt gcctttagac atgccccgca cgcgacagga actcgaggcg ctagagcgac
     2581 aaagccttgc gccttatgcg cagctaagcg ccaacacgcg cgggcgcaag caccgggaac
     2641 cggcgccgga atggcgcacg gagtatcagc gggaccgtga tcgggtcatc cactcccggg
     2701 cctttcgccg gctcgaatac aagacccagg tttttctcaa cggcactggc gatcacctcc
     2761 gcacgcggtt gacccatacg atcgaggtgg ccgccatttc gcgtaacatt gcccgtgcgc
     2821 tgaagcttaa tgaggacctc gccgaggcca ttgcgctggc gcacgacctc ggccattccc
     2881 cgttcggtca caagggtgag atggtgctca atcggctgat gaaggatttc ggcgggtttg
     2941 aacacaatcg ccaaagcctg cgcatcgtcg aggcactgga gcagcggtat ccgcggtttt
     3001 ca
//
LOCUS       DATCGS010000282         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_619406, whole genome shotgun sequence.
ACCESSION   DATCGS010000282 DATCGS010000000
VERSION     DATCGS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619406"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VIL39_05785"
     CDS             <1..203
                     /locus_tag="VIL39_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY5480971.1"
                     /translation="ADVHGILLRVAALEDTLKGKIKAWSDSARRQSKRIKDLGDIARL
                     VEAHAHLWESLPDELKQQIEHP"
     gene            complement(262..1317)
                     /locus_tag="VIL39_05790"
     CDS             complement(262..1317)
                     /locus_tag="VIL39_05790"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY5480972.1"
                     /translation="MSHTASESFEVRLASPAPLLALAPMQGVTDLPFLRLVASYGGAD
                     VYVTEYFRVYATSRLNKHILKSVTENPTGRPVIAQMIGNDIPALVRTARELQQYPVAA
                     VDLNLGCPAPVVYRKGAGGGLLREPKRVDAILGALRDAVKTPFTVKTRIGFDSAEVFS
                     ELLPIFAKHSLDLLTVHARTVKEMYRSEVHYEFIARAVAALRCPVLANGNIYSALKAA
                     EVLKITGARGLMIGRGAIRNPWLFRQIREHQRGEAIFIPRGTDVLAYVRALYEAVCLP
                     AARESAQVQKMKKYLNYFGVGVEPTGQFLHQVRRVTTTADLFRVCEEYLDHGRPMPLE
                     PFPLALKAGDLMAGEQL"
     gene            complement(1298..>2323)
                     /locus_tag="VIL39_05795"
     CDS             complement(1298..>2323)
                     /locus_tag="VIL39_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY5480973.1"
                     /translation="NFRRLIAEGALRQDPQAHFYLYRQVMGAHCQTGLVAAASCEDYL
                     KDIIRKHELTRPEKEDDRVRHIEALDSQTGPVFLVYRANQAMDKFVAGKTAEPPASDF
                     TASDGVRHTAWVIADAAGIRFIQTEFSRMPRLYIADGHHRSAAAARVFLKRKGAGESA
                     FFLSVIFPHNQMQILPYNRVMKDLNGLSPAQLLEKLAGVFEISRPGRAVPTRKHELGL
                     YLGGQWHTLRFSPRLTAATDAMETLDVTLLQKHVLEPVFGIADPRTSKRINFVGGIRG
                     TAELEKLVNSGEYACAFSMFPTSIEDLMTIADAGGIMPPKSTWFEPKLRDAMFSHLIG
                     PDVSHSK"
BASE COUNT          417 a          703 c          785 g          418 t
ORIGIN      
        1 cggccgatgt gcatggcatc ctgctccgag tggccgcgct ggaggacacg ctgaaaggca
       61 agatcaaggc ttggagcgat tcggcgcgac gccagagcaa gcgaatcaag gaccttgggg
      121 acatcgcccg gctggtggag gcacatgcgc atttatggga gtctctcccg gatgagctga
      181 aacagcagat tgaacatcca taggggaatc gaatggtctg acgagaacgt ccttggattc
      241 agtggctgaa cgatccgttc ctcacaactg ctcgcccgcc atgaggtcgc cggctttcag
      301 tgccagcggg aacggctcca gcggcatcgg ccggccgtgg tccaggtatt cctcacatac
      361 acggaacagg tcggcggtgg tcgtgacgcg gcggacctgg tggaggaatt ggccggtcgg
      421 ctccacgccc acgccgaagt agttcaggta tttcttcatc ttctgcacct gggcggactc
      481 gcgcgcggcg ggcaaacaga ctgcctcgta aagcgcgcgc acataggcca gcacgtcggt
      541 cccgcgcggg atgaagatgg cttcgccgcg ctgatgctcg cggatttgcc ggaacagcca
      601 ggggttgcga atggcgccgc ggccaatcat cagcccgcgc gcgccggtga tcttcagcac
      661 ctcggcggcc ttgagcgcgg aatagatgtt gccgttggcc agcacggggc agcgcagtgc
      721 ggcgaccgcg cgcgcgatga actcgtagtg cacctcgctc cgatacattt ccttcaccgt
      781 tcgggcatgc acggtaagca ggtcgagcga gtgcttcgca aagatcggca gcagttcgga
      841 aaagacctcc gccgagtcaa agccgatgcg ggtcttgacg gtgaacgggg ttttcacggc
      901 gtcgcgcaac gcgccgagaa tggcatccac gcgcttcggt tcacggagca ggccgccgcc
      961 cgcgcccttg cgatacacga ccggcgcggg gcaacccagg ttcaaatcca ccgccgcaac
     1021 cggatattgc tgcagctctc gagccgtgcg caccagcgcg ggaatgtcgt tgccgatcat
     1081 ctgcgcgatg accggcctgc cggtcgggtt ctcggtaacc gatttaagga tgtgcttgtt
     1141 cagccgcgaa gtggcgtaaa cgcggaagta ctccgtgaca tagacatcgg ctccgccgta
     1201 agacgcgact aatctcagga acggcagatc cgtgaccccc tgcatcggcg ccagggcgag
     1261 cagcggcgcc gggctggcga gccggacctc gaacgattca cttgctgtgt gagacatccg
     1321 gcccgatcag gtgagagaac atggcgtcgc ggagcttggg ttcaaaccag gtgctcttgg
     1381 ggggcatgat gccgcccgcg tcggcgatgg tcataaggtc ttcgatgctg gtcgggaaca
     1441 tcgagaaggc gcaggcgtat tcgccgctgt tgaccagctt ttcgagctcc gccgtgccgc
     1501 ggatgccgcc gacgaagttg atgcgcttgc tcgtgcgcgg gtcggcgatg ccgaaaaccg
     1561 gttcgagcac gtgcttttgc aggagcgtga cgtcgagcgt ttccatcgcg tcggtcgcag
     1621 cggtcaggcg cgggctaaag cgcagcgtgt gccactggcc gcccaggtaa aggcccagct
     1681 cgtgcttgcg cgtcggcacc gcgcggccgg ggcggctgat ctcgaaaacg cccgcgagct
     1741 tctccagcaa ttgcgcgggg gataggccgt tcagatcctt catcacccga ttgtagggca
     1801 ggatctgcat ctggttgtgc gggaagatga cgctcaggaa gaaggcgctt tcgccggcgc
     1861 ctttgcgctt taggaacacc cgcgccgccg cggcgctccg atggtgcccg tcggcaatgt
     1921 aaaggcgggg catccggctg aactcggttt gaatgaagcg gatgcccgcg gcgtcggcga
     1981 tcacccaggc ggtgtggcgc accccatcgc tggccgtgaa gtcgctggcc ggcggctcgg
     2041 cagtcttccc ggcgacgaat ttgtccatcg cctggttggc gcgatagacg agaaagacgg
     2101 gccccgtctg ggaatccagg gcctcgatgt gtcggacgcg gtcatcctcc ttctcgggcc
     2161 gcgtcagctc atgcttgcgg ataatgtcct tcaggtaatc ctcgcaactg gcggcggcta
     2221 ccaacccggt ttggcagtgc gcgcccatga cctggcggta gagatagaaa tgcgcctgcg
     2281 ggtcctgccg gagcgcgccc tcggcgatga gacgacggaa gtt
//
LOCUS       DATCGS010000283         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_669528, whole genome shotgun sequence.
ACCESSION   DATCGS010000283 DATCGS010000000
VERSION     DATCGS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669528"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2557
                     /gene="galB"
                     /locus_tag="VIL39_05800"
     CDS             <1..2557
                     /gene="galB"
                     /locus_tag="VIL39_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HEY5480974.1"
                     /translation="AGSEGKLDYQTLKPWLLAMGPEFSVNSPSAAKPSGNPGAEVAYT
                     QPGYDDGGWRKLNLPHDWGIEGPFKQEYPGETGKLAWWGIGWYRKHFTVPAADRGKQI
                     YLDVDGAMAYASVWVNGQFIGGWPYGYSSWRIELTPHLKPGADNVIAIRLDNPPTSSR
                     WYPGGGLYRNVWLVKTAPVHVAQWGTYITTPEISKDAATVKIEVNLDNRSERQADVKV
                     AAKVYELNAQGRKLSRAVASLAPASLAIASSSSAAATLTTTLRHPRLWSLKTPNRYVA
                     VTSVEQGGQIIDLHETPFGIRTIKFTADNGFLLNGERVALQGVCDHHDLGALGSAINT
                     RALERQIELLQEMGCNAIRTSHNPPAPELLELCDRHGLVVMDEAFDCWVRGKKKNDYH
                     LLFPDWHEKDLRALVRRDRNHPCVILWSIGNEVSEQGSPSGHKVAAELAAIVRSEDPT
                     RPVTAGCNNSQAGYNGFQKQVDVFGYNYKPGEYGKVRQANPSLPLFGSETASCVSSRG
                     EYFFPVSDDKSQGRADFQVSSYDLYAPRWAMPPDGEFRGQDEFPYVAGEFVWTGFDYL
                     GEPTPYNSDSSNLLNFTDPAEKARMEGELKELGKIQVPSRSSYFGIIDLAGFKKDRFY
                     LYQARWRPELRMAHILPHWNWPERVGQVTPVHVYTSGDEAELFLNGKSLGRKSKARYE
                     YRLRWDDVVYQPGELKVIAYKQGKRWAEEIVRTTGAAARLKLQPDRSAIQADGQDLSF
                     VTVTIADQSSRLVPRSRNRVAFELTGPGEIIAVDNGDATSFESFQAKERTACNGLCLV
                     IVRSTGEPGTLSLKARSPGLQSAEVKLQAKTGARPQTGAFDSRSLIPPTRAFK"
BASE COUNT          558 a          860 c          784 g          507 t
ORIGIN      
        1 cgccggcagc gaaggcaaat tggactacca gacgctcaaa ccctggctgc ttgccatggg
       61 acccgagttc agcgtgaatt cgccgtccgc cgccaagccg tcaggcaatc ccggcgcaga
      121 ggtcgcctac actcaacccg gttatgacga cggcggctgg cgcaagctca acctgcctca
      181 cgattgggga atcgaaggac ctttcaagca ggaatatccc ggcgagacgg gcaagcttgc
      241 gtggtgggga attggctggt atcgcaaaca cttcacagtg ccggctgctg atcgtggcaa
      301 acaaatctac ctcgacgtgg acggtgccat ggcttacgcg agcgtttggg tgaatgggca
      361 gtttatcggt ggctggccgt atggctattc ctcctggcgg atcgagctta cgccccacct
      421 caaacccggc gccgataatg tgattgccat tcggctggat aacccaccga cttcctcccg
      481 ctggtatccg ggcggcggtc tttatcgcaa cgtctggctg gtcaaaacgg ccccggtcca
      541 tgtcgcccaa tggggcacct acattaccac gcccgagatc tcgaaggatg cggccacggt
      601 aaagatcgaa gtcaacctgg acaatcgctc ggaaaggcag gccgacgtga aagtggcggc
      661 caaagtgtat gaactcaatg ctcaaggccg gaaactcagc cgcgcagttg cctccctcgc
      721 tcccgcgagt ctggcgatcg cctccagctc cagcgccgcc gcgacactca ccaccacgct
      781 ccgccatccc aggctctgga gcttgaaaac acccaaccgc tacgttgcgg tcacctcggt
      841 cgaacagggc ggccaaatca tagaccttca cgaaaccccg ttcggcatcc gcacaatcaa
      901 gttcaccgcc gataatggct ttctgctcaa tggcgagcgc gtggcgctcc agggcgtttg
      961 cgatcaccac gacctcggcg cgctcggttc ggccatcaac actcgcgcgc tggagcggca
     1021 aatcgagctg ttgcaagaga tgggctgcaa tgccatccgc accagccaca acccgcccgc
     1081 gccggaattg ctcgaattgt gcgaccgcca cggcctggtg gttatggatg aagccttcga
     1141 ttgctgggtc agaggcaaga agaagaacga ttaccatctg ctcttccccg actggcatga
     1201 gaaagatctc cgcgccctcg tccgccgcga ccgcaatcac ccctgcgtca tcctctggag
     1261 cattggcaac gaagtcagcg aacaaggctc gccttccggc cacaaggtcg cggctgagct
     1321 ggcggccatc gtccgttccg aagatcccac ccggccggtt acggctggct gcaacaattc
     1381 gcaagccggc tacaacgggt ttcagaagca agtggacgtt ttcggctaca actacaaacc
     1441 cggcgaatac ggaaaggttc gccaggccaa tccttcgctc cccctctttg gcagcgagac
     1501 cgcctcctgc gtcagttcgc gcggcgagta tttcttcccg gtcagtgacg acaagagcca
     1561 ggggcgcgcc gatttccagg tcagttccta cgacctctac gccccgcgct gggcgatgcc
     1621 gccggacggg gagtttcgcg gccaggatga gttcccttac gtggccgggg aattcgtctg
     1681 gaccgggttt gattacctcg gggagccgac gccctacaat tcggatagca gcaatttgct
     1741 taacttcacc gacccggcgg aaaaggcgcg catggaagga gagctgaagg aactgggcaa
     1801 gatccaggtg ccctcgcgca gttcctattt tggcatcatt gacctggcgg gcttcaagaa
     1861 agaccggttc tacctttatc aagcgcggtg gcggccggag ctgcgaatgg cccatatcct
     1921 gccgcactgg aactggcccg agcgcgtcgg gcaggtaact cccgtgcacg tttacacttc
     1981 cggagatgaa gccgagctgt tcctcaacgg caagtcgctt ggccgaaaga gcaaggctag
     2041 gtatgagtac cgcctccgct gggatgacgt ggtctatcag cccggcgaac tcaaggtcat
     2101 cgcatacaag caaggcaagc ggtgggccga ggagatcgtg cggaccaccg gcgctgccgc
     2161 cagactcaaa ctccagcccg accgcagcgc catccaggcc gatggccagg acctgtcctt
     2221 tgtgaccgtg accatcgctg atcagtcaag ccgattggtt ccgcgctccc ggaaccgcgt
     2281 ggcttttgag ctgaccgggc ccggcgaaat catcgccgtg gataacggtg atgcgaccag
     2341 tttcgagtct tttcaggcca aagagcgcac cgcctgcaac gggctctgcc tcgtgattgt
     2401 ccgttctacc ggtgaacccg gcacgctgag cctcaaagcc cgttcccccg gcttgcagag
     2461 cgccgaggtt aagctccaag ccaagactgg tgctcgacct caaaccgggg ctttcgattc
     2521 gagatccctt attccgccta cccgcgcgtt caagtaagac gacgacagat gacaaatcta
     2581 agcaggcagc aagcccttgt ctttcccgct gggccaagag aagacagaac ctcctactca
     2641 ctaccacgac ggaggtgtgg caaggtcgtt gctgctctgc tggtggctgg gctgctgggt
     2701 tgcttattc
//
LOCUS       DATCGS010000284         5563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_272704, whole genome shotgun sequence.
ACCESSION   DATCGS010000284 DATCGS010000000
VERSION     DATCGS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272704"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VIL39_05805"
     CDS             <1..408
                     /locus_tag="VIL39_05805"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEY5480975.1"
                     /translation="GLDFCTSTGAINDKAPWGDIQEVFYELKAPTDRNEAGMELVRCI
                     NRNLLATTTQTPEIQFLMGKVQTLQFDCYDGSQWRNTWDTSAGDTNLPVAVRIRIQLV
                     AKEGEGAGNLQPMEMLVALDSQTRTNLTTTGTQ"
     gene            405..1856
                     /locus_tag="VIL39_05810"
     CDS             405..1856
                     /locus_tag="VIL39_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEY5480976.1"
                     /translation="MRTRANIEVFHRAQRGSVLVIVLWIAFGLVSLALYFANSMNFEL
                     RASDNRVSAMAADQAIEGAERYINYLLTQQVINGSNGVFLALNDAQCRDVPVGDAHFW
                     IIGRDTNNPVGPGQMCFGLVDEASKLNLNMAASHPLSTSNQIIWLPRMTADLTQAILD
                     WVSTNGNGPTVTYYAMQQPAYQCKCDLFETVDELRLLYGADMDTLVGEDANRNGILDP
                     NETDDNQNGMLDPGVLEYFTVYTREPNTNSSGTARVNVSTLTSGSTELISLLQTNFGD
                     ARSTQILGNLGLVSAPSRGPSGGASGTSGGGGRSPAPGGTTTVTNKSPLEFYVKSGMT
                     SAEFALIGTNLTTTSGSYVEGRVNINTASAAVLACLVGGDTGVAQQLVNYRQSNPNNL
                     TTIAWIIDALGQSYPEALTALEARDYITTQSYQFTADIAALGPYGRGYRRVKFIFDTS
                     DGTPRIVYRQDLTHLGWALGKEVRQNLLSAKKT"
     gene            1856..3316
                     /locus_tag="VIL39_05815"
     CDS             1856..3316
                     /locus_tag="VIL39_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480977.1"
                     /translation="MIKLPKSDLLKRQRLSSLLGLALDGSRLDGLALRRADGALHIQQ
                     VFSVSLSLDPLTNDPELVGREIRNHLDAAGVREHHCIVGLPLKWALTTHIEVPELPEA
                     DIPGFLQIEAERNFPCDVQTLHVVTSRSHTPAGKQHATLVGIPKNHLARLEQVLRAAK
                     LKPLSFSLGIAALQPPETDASNGIMALAIGETHVALQVTVGGGVAALRALEGALEAEG
                     SQRVLHADLIAREARITLGQLPAELRETVRRIRVFGPRDLAQQLVDELELRLDATGLK
                     VESVTRYAANEFGAQLPPDAPVSPAFSLAASRLAGRRALFEFLLPKVTSLQQLAARYS
                     SGRLRTVILAAAAVALVAGGLFFFQQCQLWRLESQWAKLQPTVRQLEGMQEQIRQYRP
                     WYDESVRGLTILKRLTEAFPEDGSVTAKTVEIRDLNTITCTGIAKNYQALLKTVEKLR
                     SIRQIPDVNLGPTRGQPPALQFTFNFQWSEGGRSAN"
     gene            3306..3845
                     /locus_tag="VIL39_05820"
     CDS             3306..3845
                     /locus_tag="VIL39_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480978.1"
                     /translation="MQIKNRQQLLIIGAIVAVALFAGDQLVLSPLIKVWSARATRVTE
                     LRKKITQGKMLVDREQKQSIRSRWEQMVTNALPNNTSAAEQQVFKAIDLWAQNSGVAI
                     SAITPQWKHDSDDYMTFECRVDAAGDLGKLSRFLYSVERDPMALKLEFVELGALDKEG
                     QQLSLGLQLSGLVLNTPTR"
     gene            3851..4849
                     /locus_tag="VIL39_05825"
     CDS             3851..4849
                     /locus_tag="VIL39_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480979.1"
                     /translation="MRNIKLIVWELIVDGSRLWSAIGFAVRMTTLWLALICGFPVVAQ
                     EPDASAQTNDMTAAEVLSQLNAMVQATDGAQAEDMAPSNEPAATNGLPQASAPASGGD
                     RPNRFIRPDNSTPSPGSSRPQSNDRRSRGRRSSNSRPGQPGGYGSARDYSSGSDRTPT
                     NAATGTNNGLVSLDYSAFKMIVDRNIFDPNRFPHAPGQVRPTSKPRSVDYVTLVGTMS
                     YEKGTFAFFDGTSSDYKKALKLTDSIAGYKVTNITPNSVKLAAGTNELNLNVGAQLRR
                     EENGPWLLASQFTSYAATPASTSTNGTAATTTGSDTTSGGTESDILKRLMQKREKE"
     gene            4856..>5563
                     /locus_tag="VIL39_05830"
     CDS             4856..>5563
                     /locus_tag="VIL39_05830"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480980.1"
                     /translation="MKTINHLLLLACLSAGIPILAAESTPGAPTNEAPSAPATAIQPS
                     TGQPPPAPTPAPPDATNSAAVPATPAPGPGVAIDAIAAADAVTGTNTVSAAPPAAPTI
                     VVETGTNGMRLNFHGAPLSLVLDYLSDAAGFIINKETDVRGTVEVWSKEPLTKDEAVE
                     VLNSVLKKNGCAVIRNGRILTIVAQDTAKTRDLPVISGNDPNEVEKGDEVVTQIIPVK
                     YATVSQLVPNLELLLPTT"
BASE COUNT         1149 a         1761 c         1688 g          965 t
ORIGIN      
        1 gggctggatt tctgcacttc gaccggcgcg attaacgaca aggcgccatg gggtgatatt
       61 caggaagtgt tctatgagtt aaaggcgccg accgaccgga acgaggcggg gatggaactg
      121 gtgcgttgta tcaatcgcaa cctgctggcc accaccaccc agaccccgga gatccagttc
      181 ctgatgggca aggtgcagac gctgcaattt gactgttatg acggctcgca gtggcgcaat
      241 acctgggata ccagcgccgg cgataccaac ctgccggtgg cggtccgaat ccgcattcag
      301 ctcgtcgcca aggaaggtga aggggccggt aacctgcagc cgatggaaat gcttgtcgcc
      361 ctggactcgc agacgcgtac caacctaacc accacgggga cgcaatgaga actcgcgcca
      421 acattgaggt ttttcatcgc gctcagcggg gttccgtgct cgttattgtg ctctggatcg
      481 cgttcgggct ggtgagcctg gcgctgtatt ttgcgaactc gatgaacttc gaactgcgcg
      541 cctcggacaa ccgggtgtcc gcgatggcag cggaccaggc gatcgagggc gcggagcgct
      601 atatcaatta cctgctgact cagcaagtta tcaacggttc caacggcgtc ttcctggccc
      661 tgaacgacgc ccagtgccgg gacgtcccgg tgggtgatgc ccatttctgg atcatcgggc
      721 gcgacaccaa taacccggtc gggcccggcc agatgtgctt tggcctggtg gacgaggcct
      781 caaagctcaa cttgaacatg gccgccagcc acccgctctc caccagcaac cagatcatct
      841 ggctgccgcg gatgacggcg gacttgaccc aggccattct cgattgggtg agcaccaacg
      901 gcaacggacc cacggtcacc tactacgcca tgcagcagcc ggcttaccag tgcaagtgtg
      961 atctctttga aaccgtggac gagctgcggc tcctgtatgg cgcggacatg gacacgctgg
     1021 tgggcgagga tgccaaccgc aacgggatac tcgacccgaa cgagacggac gacaaccaga
     1081 atggcatgct tgaccccggg gtgctggaat acttcaccgt ctatacccgg gagcccaaca
     1141 cgaacagcag tgggaccgcc agggtcaacg ttagcaccct caccagcggg tccaccgagt
     1201 tgatctcgct cctgcagacc aacttcggcg atgcgcggtc cacccagatc ctcggcaatc
     1261 tcggcctggt gagcgctccc tcccggggtc cgagtggcgg cgcctcaggc acatcgggcg
     1321 gcggcggccg tagcccggcg cccggcggca cgacgacggt cactaataag agcccgttgg
     1381 agttttatgt gaagagcggc atgacgtcag ccgagtttgc tctaatcggc acgaacctca
     1441 ccacaaccag cggcagttac gtcgaggggc gagtgaacat caatacggcc agcgcggcgg
     1501 tgttggcttg cctggtcgga ggcgatacgg gggtggcgca gcaactggtc aactatcgcc
     1561 agtccaaccc gaataatctg acgacgattg cgtggattat tgatgcgctg ggccagagct
     1621 atccggaggc gctcacagcc ctggaggccc gggattatat tacgacgcaa agctaccagt
     1681 ttaccgccga tatagccgcc ctcggaccct acggccgcgg gtatcggcgg gtgaaattca
     1741 tcttcgacac cagcgacggc acacccagaa tcgtgtatcg tcaggacctg acccatctcg
     1801 ggtgggcctt gggcaaagaa gtgcgccaga acctgctctc agcgaagaaa acgtgatgat
     1861 caaactgccc aaatcagact tattgaaacg acagcgcctc tcgtccctgc tggggctggc
     1921 gctggacgga agccgcctgg atgggctcgc cttgcgccgc gccgacgggg ccctgcacat
     1981 ccagcaagtc ttctccgtct cgctttcgct cgaccccctg accaatgatc ccgaactggt
     2041 tggccgggag attcgcaacc acctcgacgc cgccggcgtg cgcgagcacc attgcatcgt
     2101 gggcctgccg ttgaagtggg cgctgacgac gcacatcgag gtgcccgagc tacccgaggc
     2161 ggacatcccg ggctttctgc aaatcgaggc ggagcgcaat ttcccgtgcg acgtgcagac
     2221 gctgcacgtc gtcacttccc gctcgcatac cccggctggc aaacaacacg ccacgctggt
     2281 cggcattccg aagaaccatc tggcgcggct ggaacaggtg ctgcgggcgg cgaagctgaa
     2341 gccgctcagc ttctcgctgg gcatcgcggc gctgcaaccg cctgagaccg acgcctcgaa
     2401 cggcatcatg gccctcgcca ttggcgaaac ccacgtggcg ctgcaagtta cggtcggcgg
     2461 cggcgtggcg gcgctgcggg cgctggaagg cgcgctcgaa gccgaaggca gccagcgagt
     2521 gctgcacgcc gatctgatcg cgcgcgaggc ccgcatcacg ctcgggcaac tgccggcgga
     2581 gttgcgtgag acggtgcggc ggattcgggt cttcggcccg cgggacctgg cccagcagtt
     2641 ggtggatgag ctggaactgc ggctggatgc gactggcctg aaggtcgagt ccgtgacgcg
     2701 ttatgccgcc aatgaatttg gcgcccagct tcccccggat gcccccgtgt cgccggcgtt
     2761 cagcctggcc gccagccggc tagccgggcg gcgcgcgctg ttcgagttcc tgctgcccaa
     2821 agttacctcc ttgcagcaac tggccgcccg ctattcttcc ggcaggctcc gcacggtgat
     2881 cctggcggcc gcggcggtcg cgctcgtggc cggggggctg ttctttttcc agcagtgcca
     2941 gctctggcgg ctggagtcgc aatgggcaaa gctccagcca accgtccggc aactggaggg
     3001 catgcaagaa caaatccggc aataccgccc ctggtacgac gaatccgtgc gcggcttgac
     3061 tatcctcaaa cgtctcacag aggccttccc cgaagacggc agcgtgacgg ccaagaccgt
     3121 ggagattcgt gatttgaaca cgatcacttg cacgggcatc gccaagaact accaggccct
     3181 gctgaagacg gtcgaaaaac tgcgcagcat tcggcaaatt cccgacgtga acctgggtcc
     3241 aactcgcggg caaccgccag cgttgcagtt tacgttcaac tttcaatgga gtgaaggagg
     3301 aagaagtgca aattaagaat cgtcagcaac tattgattat cggggcgatt gtagctgtcg
     3361 cgctgttcgc aggggaccaa ctggtcctct cgcccctgat caaggtctgg agcgcgagag
     3421 cgacgcgcgt taccgagctg cgcaagaaga ttacccaggg caaaatgctc gtggaccgcg
     3481 agcaaaagca aagcatccgg agccgctggg agcagatggt caccaacgcg ctgccgaaca
     3541 acacctccgc ggctgaacag caggtcttca aggcgattga tctgtgggcg cagaacagcg
     3601 gtgtcgctat ttccgccatc actccccagt ggaagcacga cagcgatgat tatatgacct
     3661 ttgaatgccg cgtggacgcg gcgggcgacc tcgggaaact gagccgcttc ctctacagcg
     3721 tcgaacggga cccgatggcg ctgaagctgg agttcgtgga actgggcgcg ctcgacaagg
     3781 aaggccagca gctttcgttg ggtctgcaac tcagcggctt ggtcctcaat acccccacac
     3841 gatgaacccg atgcgaaaca ttaagttgat agtttgggag ttgatagttg atggcagcag
     3901 actctggagc gcgattggct ttgctgtccg catgaccacc ctatggctcg ccttgatctg
     3961 tggcttcccg gtcgtggcgc aggaaccgga tgcctccgcc cagaccaatg acatgacggc
     4021 ggcggaggtc ctgtcccaac tcaacgccat ggtccaggcc accgacggcg cacaagccga
     4081 ggacatggct ccgtccaacg aaccggcggc gaccaacggt ctcccgcaag ccagcgctcc
     4141 ggcgtccggc ggggaccgcc ccaacaggtt tatccgtccc gacaactcga ccccctcccc
     4201 gggctccagc cgacctcaga gtaatgatcg ccggtctcgc gggcggcgtt cgtccaattc
     4261 aaggcccggc cagcccggtg gttacgggtc ggccagggat tacagctcag gcagcgaccg
     4321 taccccgaca aatgccgcta ccgggacgaa caatggcctc gtctcgctgg actactccgc
     4381 cttcaagatg atcgtggacc ggaatatctt cgaccccaac cggtttccgc acgctccggg
     4441 acaggtgcgc cccacgtcaa agcccaggag cgttgattat gtgacgctgg tcgggaccat
     4501 gagttatgaa aaaggcacgt tcgccttttt tgacggcacg agttccgact ataagaaggc
     4561 gctgaagctg accgactcga ttgcgggcta caaggtcacc aacattacgc cgaacagcgt
     4621 gaagctggcg gctggaacca acgaactgaa tttgaacgtg ggggcgcaat tgcgccgcga
     4681 ggagaacggg ccctggctgc tggccagcca attcacgtcc tatgcggcca cgcccgcttc
     4741 gacctccacc aacggcacag ccgccaccac cactggctca gataccacat ccgggggcac
     4801 ggagagcgac atacttaaac gactgatgca gaaacgcgaa aaggaataat tcccaatgaa
     4861 aacaatcaat catttgcttc tattagcgtg cctgtccgca ggcatcccga tcctggccgc
     4921 ggaatcaaca cccggcgctc ccaccaatga agcgcccagt gcaccggcca ctgctatcca
     4981 gccgagcact ggtcaaccgc cgcccgcgcc aaccccggct ccgccggacg ccaccaactc
     5041 agccgccgta cccgccacgc cggccccggg tccaggcgtc gcgattgacg ccattgcggc
     5101 tgccgacgca gttactggca cgaacacagt ctctgccgca ccgccagccg cgccgacgat
     5161 agtcgtcgag actggcacga atggaatgcg cctgaacttt cacggggcgc cgctgagcct
     5221 ggtgctggat tatctgagcg atgccgccgg cttcatcatc aacaaagaga ccgacgtccg
     5281 cggcacggtc gaagtctgga gcaaggagcc cctgacgaag gatgaagcgg tcgaggtgct
     5341 taattcggtg ctgaagaaga acgggtgcgc cgtcattcgc aatggccgca tcctgaccat
     5401 tgtcgcccag gatacggcca aaaccaggga ccttcccgtc atttccggca acgaccccaa
     5461 cgaggtcgaa aaaggggatg aagtggtgac ccagatcatt ccggtgaagt acgccaccgt
     5521 gagtcagtta gtgcccaacc tggaactgct tctgccgacc acg
//
LOCUS       DATCGS010000285         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_323158, whole genome shotgun sequence.
ACCESSION   DATCGS010000285 DATCGS010000000
VERSION     DATCGS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323158"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(500..1702)
                     /locus_tag="VIL39_05835"
     CDS             complement(500..1702)
                     /locus_tag="VIL39_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5480981.1"
                     /translation="MSRAIKIIAAGLLALCASSWARAEEGVLRVGIITDMSGQYADGN
                     GPGAVIAAQMAAEEIGGTVSGRHIEIISADHQNKPDVAAAIVRNWIDNKSIDVIAEGV
                     NSSVALAIQNLTRERKKVFLISGSGSSDLTGKQCSPTSVQWTYDTYASSNSTAKAVVA
                     RGGTPWFFLTADYAFGHALERDASKAVIAAGGKVLGAVRHPFNTADFSSFLLQAQASG
                     AKILALANAGADLRNTVGQADEFNIRQSMQLVALQVTLTDVPAIGLAHAHDLLFTDSF
                     YWDRTPETRAFSNEFFKRHSAMPTAYQAGVNSALRHYFKAVAATNSTESETVIAQMRK
                     MPVDDFFAQGGKVREDGRMVHDMYLMRIKRPEESKQKWDLYEYLATVPGDEAFRPMAE
                     GDCPYLAK"
     gene            complement(2858..4099)
                     /locus_tag="VIL39_05840"
     CDS             complement(2858..4099)
                     /locus_tag="VIL39_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY5480982.1"
                     /translation="MANAGQALPRAYDVIVYGASGFTGRLVAEHLLGTYGATGEIRWA
                     MAGRSTGKLAEVRDLIGGPSNFPLIAADASDPASLKTMAENAKVIISTVGPYQLYEGL
                     VAACAAAGTDYVDLCGEPAWMAAMIRKYGDAAQASGARIVFSCGFDSIPFDLGVFFLE
                     QQARAKFGQPATRVRGRVRKMKGTFSGGTAASLLATVEAAAREPAIRKTLSDPFALTP
                     GFVGAPQPSGNEVVEDVVAGSWVAPFMMAMINTKAVHRSNQLLNYAYGRDFVYDEMMM
                     TGSGSTGEKRAKAAARREAVQNALIGFAPTRALIRRFVLPKPGQGPSKAARDAGFYDI
                     LLIGETSDGRSLRVSVKGDKDPGYGSTSKMIAESGICLAREVSHEAVGGGFWTTASSM
                     AEPLIGRLQAKAGLTFTVENG"
BASE COUNT          820 a         1253 c         1189 g          838 t
ORIGIN      
        1 gtgtggattt atctaggaca tcaatcgacg cgctgatgaa ttgccctgct atgggcaacg
       61 gccctgccgg gctggttcgg gcagggagca gcgccccaaa aggcaaaggt catacgttca
      121 aatcgtgtcg ggtgcgccag gaaagcacca gccgagggtg ccgggactgt ggctggtctg
      181 gcccctggcc ggctttccct ctgccggaaa ttcgctgttt ttctagcgcc gatggcccag
      241 acgaattccg agcttgaggg ccttctgtcg cagcgatcct tcacttcgct tcgtgagctt
      301 cgcgattctc gcgaccggtg ttttcgcttt ggagtgcaca cgcagctcct tcacatcagc
      361 ctttgtgtac tcgcgacgca caacagcctg ctttgctttc ttagccacgt actattcctt
      421 gatggtgagg cgttgttact atcagaaaac tcagacgaaa caaccgtcct tacacatccg
      481 accgagagca tcggtcccct tacttagcca gataggggca gtccccctca gccattggcc
      541 gaaacgcctc gtcgcccggc acggtcgcga gatattcgta aaggtcccac ttctgttttg
      601 attcttcggg cctcttaatt cgcatgagat acatgtcatg aaccatacgc ccgtcttcgc
      661 gcacttttcc gccctgcgcg aagaaatcgt ccactggcat tttgcgcatt tgggcgatca
      721 cggtctcgga ctcggtggag ttggtcgcag ccaccgcctt gaaatagtgc cgaagcgccg
      781 aattgacacc ggcttgatag gccgtcggca tggcgctatg ccgcttaaag aactcgttac
      841 tgaaagcgcg cgtctcgggc gtgcgatccc agtaaaagga gtcggtaaag agaagatcat
      901 gtgcatgcgc cagaccgatc gccggcacgt cggtcagcgt cacctgcaaa gcaacgagct
      961 gcatgctctg gcggatgttg aactcgtcgg cctgccccac cgtgttacgc aaatccgcgc
     1021 cggcattggc gagagcgaga attttcgcgc cggatgcctg cgcctgcaac aggaacgacg
     1081 aaaagtccgc ggtgttgaag ggatgacgga ccgcgccaag cactttgccg cctgctgcaa
     1141 tgaccgcctt gctcgcatca cgctccaatg cgtggccgaa agcataatcg gcggtaagga
     1201 agaaccacgg cgtgccgccg cgcgcgacaa ccgccttcgc agtcgaattg gaggaggcgt
     1261 aagtgtcgta ggtccattgc acgctagtcg gagagcattg cttgccggtg agatcggacg
     1321 agccagagcc cgaaatgaga aacacctttt ttcgctcgcg ggtcaggttc tggatggcca
     1381 gcgcaacgga ggaattgacg ccttcggcga tgacatcgat acttttatta tcgatccagt
     1441 tgcgcacgat agcggcggct acgtctggct tattctgatg atcggcagaa ataatttcga
     1501 tgtggcggcc cgagaccgtt ccgccgatct cttccgccgc catttgagcc gcaatgacgg
     1561 cgcccggacc gttaccgtcg gcatattggc cactcatgtc ggtaatgatg ccaacacgca
     1621 gcacgccttc ttcggcacgc gcccaacttg aagcgcaaag tgccaacagg ccggctgcaa
     1681 ttatcttgat cgcacgagac attactcggt ttcctccctg gaagcctcca gtgaggccat
     1741 ttaccggacg ccagtggctg ttcgcaagct tgcggattcc gaaaggctct tcggcatagc
     1801 agacatcttc gatggcataa aacttcgacg cgagcggcca cacaattagg gcggggttgg
     1861 tcaaccgcga gccgctcggg cacgagcggt ggtccgagta gatataaggt gccgtggagg
     1921 cggcttcgtt cctagatgtt gtaggttccg cgcggtagtt ggggcctgaa cgccctccaa
     1981 ggtggaaaag aaaattggaa tcgaagagaa tcgaaggaaa cctttttcat cctaataact
     2041 gaatcggccg gctatttttc ccaaccggcc ttttgctacc ccagacccac cgatcgcgct
     2101 ggcattaggc gtgcctatag agcttcaacc aggcttccga ttccccttag ccacgacaac
     2161 cgccttcgct gaaacgatgt attagaccat acacagcagg cacttcctta ttccaggctc
     2221 tctcgctggt cgcatcacca aatagcattg gcgacattcc ccgactttcc gaggttacgg
     2281 cgctcaacgg gtgctgggcc tccttggggt tcgaccggcg catcgggctc ggactggccg
     2341 accgggattt tggttagttt tttggtttgc agtgcagcca gattctacca actgcaaacc
     2401 agcagcaacc ggattttggc tgcagctaac cgccagcttt gggccagatg acccactaaa
     2461 tgtagagctg ggccggagcc ccgggaggtg ttcgagagct gccgaagcca cccgatcggg
     2521 tatggacatc ccggacctca cccctgacca ggattgccgc ttcacacacc ggaccgatgg
     2581 tggcatcgcc atcagccggg cgcgtctgag tttggggcta cctccaagga ggatgagaaa
     2641 aatactggtg gcgacaggct cgatccctgt ggcccgaacc ggcgttctct aggagttcga
     2701 tgctccacct gctgacgcgg gtgtatctgc gcgtatcttg atggcaccgt tcagacctga
     2761 ccggtcgaac tgggcaaagc ggacctggcg actcgagcgt caacttccga aattgacccc
     2821 ggtggagaca ttcagcgcca cagcttccaa gtccggacta accattctcc accgtgaagg
     2881 tgagaccggc ctttgcttgc agccggccga tgagcggctc tgccatcgaa gaggctgtgg
     2941 tccagaatcc tccgccaacc gcctcatgcg agacctcgcg cgctagacag atgccgctct
     3001 cggcgatcat cttggacgtc gaaccgtagc cggggtcctt gtcacccttg accgagaccc
     3061 tcaggctgcg gccatcgctg gtctcgccga taagcaggat gtcgtagaag ccggcgtcgc
     3121 gggccgcctt ggatgggccc tgcccgggct tcggcaggac aaaacgacgg atcaaggcgc
     3181 gggtcggtgc aaacccgatc agagcgttct ggaccgcctc gcggcgtgcc gccgctttgg
     3241 ctcgcttctc gccagtcgag ccggagcccg tcatcatcat ctcgtcatag acaaaatctc
     3301 ggccataggc gtagttcagc agctggttcg aacgatgcac cgccttggtg ttgatcatcg
     3361 ccatcatgaa gggcgcaacc cacgagcccg cgacgacgtc ctcgacgacc tcgtttccac
     3421 tcggctgagg cgctcctaca aatcccggcg tcagggcgaa gggatcggac agagtcttgc
     3481 ggatcgcagg ctctcgcgcg gcggcttcga cggtcgcgag caggcttgcc gcagtgccgc
     3541 cggaaaatgt gcctttcatt ttccgcaccc ggccgcgcac ccgtgttgcg ggctgcccga
     3601 actttgcccg cgcctgctgc tcgagaaaga agactcccag atcaaaagga atggagtcga
     3661 agccacacga aaacacgatg cgcgcgccgg atgcttgcgc ggcgtcaccg tatttacgaa
     3721 tcatcgccgc catccaggcc ggctccccgc acaggtccac atagtcggtt ccggccgcgg
     3781 cacaggccgc cacgaggccc tcgtaaagct gataaggccc gaccgtcgaa atgatcacct
     3841 tggcgttttc cgccatggtt ttcagcgaag ctggatcgga cgcgtcggcg gcgatcagcg
     3901 ggaaattcga cggtccccca atgaggtcgc ggacctcagc gagtttcccg gtgcttcgtc
     3961 cggccatcgc ccacctgatt tcgcccgtcg cgccataggt gccgagcaga tgttcagcca
     4021 ccagtcggcc ggtaaagccg gacgcgccat agacgatgac gtcataggcg cgcgggagcg
     4081 cttgtccggc attggccatg
//
LOCUS       DATCGS010000286         3238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_370778, whole genome shotgun sequence.
ACCESSION   DATCGS010000286 DATCGS010000000
VERSION     DATCGS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370778"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VIL39_05845"
     CDS             <1..1125
                     /locus_tag="VIL39_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5480983.1"
                     /translation="TQIPVTTTLMGCGAFPETDPLSLRWLGMHGAAFANWAVSGEFEL
                     HKNPADPVKLIAPGADLLLAFGVRFDDRVTGKVDKFCEKGTIVHLDIDPSEINKNKPA
                     NLPIVSDVKFALGRLAEMVKERPIQKKFTAWHKQIEAWKAKAPFGYLVTDEVMKSQHM
                     RDHLKGRESEVILPQMAIETLYELSKGEALITTGVGQHQMWTGQFYKFKYPRQFLTSA
                     GLGAMGYGYPAALGAKVAFPDRQVVDIDGDGSFLMNVQELATAHIEKIAAKAIIMNNQ
                     HLGMVMQWEDRFYKGNRGNTYLGDPQNRKEIYPDYTAVCNSFNVKCERVMYQRDLRAA
                     MQRMLDANEPYVLDIIVPYTEHVMPFIPANKSVAEMIWQA"
     gene            complement(1180..2232)
                     /gene="lpxD"
                     /locus_tag="VIL39_05850"
     CDS             complement(1180..2232)
                     /gene="lpxD"
                     /locus_tag="VIL39_05850"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY5480984.1"
                     /translation="MPYTVAEIARLLGGEVVGDASLVLKGFAPADRAQAGDLTFAENE
                     SYFARAEQSAASTIIIDGPVASSRKTLIRVANARVAFAKALPLFFPEPAFAAGIHPTA
                     IVPSSAQVDPTAHIGPHCILGEKVRIGPRSVLQGLNYVGADSLIGEDVNLFPNVTLYP
                     RTEVGNRVRIHSGTVVGSDGFGYVLDNGVHRKVPQIGNVIICDDVELGANVTVDRGAL
                     GPTIVGKGSKIDNLVQIAHNVIVGEHCIIVSQVGVSGSTKLGNYVVLGGQVGLAGHLK
                     IGNRVSVAAQSGVMNNIPDGEKWLWTPAQPDRQAKRQMIALQQLPELIRRVKQLEKQL
                     GLKAEPAAAPESGRPK"
     gene            2449..>3238
                     /locus_tag="VIL39_05855"
     CDS             2449..>3238
                     /locus_tag="VIL39_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5480985.1"
                     /translation="MSNRPLNVGLVGGGKGAFIVHPHERAIHFDGTRRVVAGALFPDP
                     KIALEEAANWPYPIKGYGSYDEMIAANATLPADQKLDYILIVTPNFVHFDPAMKAMKA
                     GIAVFCEKPLCLNLKEASELVQTSRKLKIPFGVSHTYLGHWTSRLSRYIVRSGLLGEV
                     RWVDSSYLQGWLATKLEATGQTQAVWRVDPKRAGGSGCGGDIGTHALMQLRYATGLNV
                     TQVSAQLETFVEGRQLDDHFTVYCKLSNGGKALVRASQICIGFKN"
BASE COUNT          666 a          984 c          991 g          597 t
ORIGIN      
        1 acgcagatcc cggtcacgac gacgctgatg ggctgcggtg cgttcccgga gacggacccg
       61 ctgtcgttgc gctggctcgg catgcacggt gcggccttcg cgaattgggc ggtgagcggc
      121 gagttcgagt tgcacaagaa tccagccgac cccgtgaagc tcattgcgcc cggagccgat
      181 ttactgctgg catttggcgt gcggttcgac gatcgcgtga ccggcaaagt ggacaagttc
      241 tgcgagaagg ggaccatcgt tcacctcgac attgacccgt cagagatcaa taagaacaag
      301 ccggccaacc tgcccatcgt cagcgatgtc aagtttgccc tggggcgact ggccgagatg
      361 gtcaaagagc ggccgatcca gaagaagttc acggcctggc acaagcagat tgaggcgtgg
      421 aaagccaaag cgccgttcgg ctacctggtg accgatgagg tcatgaagtc acagcacatg
      481 cgcgaccacc tgaaaggcag agagagcgag gtgatcctgc cgcagatggc cattgagacc
      541 ctgtacgaat tgtccaaggg cgaggctctc atcaccacgg gcgtgggcca gcaccagatg
      601 tggacggggc aattctacaa gttcaaatac ccgcgccagt tcctgaccag cgccgggctg
      661 ggcgcgatgg gttacgggta tcccgcggca ttgggggcca aggtggcgtt ccccgaccgg
      721 caggtggttg atattgatgg cgacggctcg ttcctgatga acgttcagga gctggccacc
      781 gcgcacatcg agaagattgc ggccaaggcg atcatcatga ataaccagca cctgggcatg
      841 gtgatgcagt gggaagaccg gttttacaag ggcaaccgcg gcaacaccta cctgggcgac
      901 ccgcagaacc ggaaggagat ctatcccgat tacaccgccg tgtgcaatag cttcaacgtg
      961 aagtgtgaac gggtcatgta ccaaagggac cttcgggcgg cgatgcagcg catgctggac
     1021 gccaacgaac cctacgtgct ggacatcatc gtgccctaca ccgagcacgt gatgcctttc
     1081 atcccggcca ataagagcgt cgccgaaatg atctggcagg cgtaggctag aaggcttgag
     1141 cgggctcggc ctccgagccc gcgacgcaga tcgcagcgtc tatttcggcc gtcccgattc
     1201 cggtgcagcg gcgggttccg ctttcagccc gagctgtttc tccaactgtt tgacacggcg
     1261 gatcagttcg ggcaactgct gcagcgcaat catttgccgc ttggcttgcc ggtcgggctg
     1321 cgccggggtc cagagccatt tctcgccgtc cgggatgttg ttcatcactc cggactgggc
     1381 cgccaccgag acacggttgc cgattttcaa gtgaccggcc agtccaacct gcccgccgag
     1441 gaccacgtag ttgcccagct tggtgctccc ggatacgccg acttgcgaaa cgatgatgca
     1501 atgctccccc acgatcacat tgtgcgcgat ctggacgagg ttatcgatct tcgagccttt
     1561 gccgacaatg gtcggcccga gcgcgccgcg atcaaccgtc acattggcgc ccagttccac
     1621 gtcgtcgcag ataatgacat tgccgatttg gggcaccttg cgatggacgc cgttatcgag
     1681 cacgtagccg aatccatccg agccgacgac cgtgccggag tggatgcgca cccggttgcc
     1741 cacctcggtg cgaggataga gcgtgacgtt ggggaagagg ttgacgtcct cgccaatcag
     1801 ggagtcggcg ccgacatagt tgaggccctg caatactgag cgcggcccaa tccgcacctt
     1861 ctcgcccagg atgcaatgcg gcccgatgtg cgccgtggga tcgacctggg cgctggacgg
     1921 cacaatcgcc gtggggtgga tgccggcggc gaaggccggc tccgggaaga acagcgggag
     1981 ggctttcgca aacgcgacgc gggcgttggc cacgcggatg agggtcttgc ggctggaggc
     2041 aacgggaccg tcaatgatga tcgtcgaggc cgcgctctgt tcggcccgtg cgaaatagct
     2101 ctcgttctcc gcaaaggtga ggtcgcccgc ttgggcgcgg tccgccgggg cgaagccttt
     2161 gagaacgagc gaagcgtccc cgactacttc gcccccgaga agtctggcaa tttcagcaac
     2221 ggtataaggc ataaggcatt gacttggaca accgcggcca ctataaaggc cacggcagct
     2281 aattcaaccc ttactttgaa ggcgggctgt gaggatctgg ccttgaactc aactcggccg
     2341 ctctgtagcc tgcgcgcaag ttttggctgg cagaaacata agctaacgac agacaataga
     2401 ctcatcatta atcactagtc accaattaca caaacaggag aaatatatat gtccaacaga
     2461 ccccttaatg tcggtttagt cggcggcggc aaaggcgcct tcatcgtcca cccgcacgag
     2521 cgagccattc acttcgacgg cacccgccgc gtggttgccg gcgcgctgtt tcctgatccc
     2581 aaaatcgccc tcgaagaggc cgccaattgg ccttacccca tcaagggcta tggctcctat
     2641 gacgagatga tcgccgctaa cgccactctg cccgcggacc agaagctgga ctacatcctc
     2701 atcgtaacgc ccaacttcgt gcatttcgat ccggcgatga aggccatgaa ggccggcatc
     2761 gcggtgttct gcgagaagcc gctctgcctg aatctcaaag aggcgagcga gctggtacag
     2821 acctcgcgca agctgaagat cccgttcggc gtctcccaca cttacctcgg ccattggacc
     2881 agccgcctca gccgctacat tgtccgcagc ggtttgctgg gcgaagtgcg ctgggtggac
     2941 agctcctacc tccaggggtg gctggcgacc aagctcgaag ccaccggcca gacccaggcg
     3001 gtctggcgcg ttgatcccaa gcgcgccggc ggctccggct gcggtggcga catcggcacg
     3061 cacgccctga tgcaactccg ctacgctacc ggcctcaatg tcacacaggt tagcgcgcag
     3121 cttgagacgt ttgtcgaagg ccgccaactc gacgaccact tcaccgttta ttgcaagctc
     3181 agcaacggcg gtaaagccct cgtgcgcgcc tcgcagattt gcatcggttt caagaatg
//
LOCUS       DATCGS010000287         5210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_175019, whole genome shotgun sequence.
ACCESSION   DATCGS010000287 DATCGS010000000
VERSION     DATCGS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175019"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..317)
                     /locus_tag="VIL39_05860"
     CDS             complement(48..317)
                     /locus_tag="VIL39_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480986.1"
                     /translation="MQIDYTVQVWREDGQFIAHAMPLDVASSGPTPEAARKAVDEAVR
                     LFLATAAEHGTLTEVLEDAGYRRVRREWRGPAWLGFEQRSCLAEV"
     gene            867..2060
                     /locus_tag="VIL39_05865"
     CDS             867..2060
                     /locus_tag="VIL39_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY5480987.1"
                     /translation="MNIHFVTGQEKDLDLIAGAGFKFVRMDFHWEAIETSKGEYNWAG
                     YDDLLANLEKRGLRAILILDYSHHLYEDSVTSPHPITGQAHKTTSSPQHPASIAAFAR
                     WAAASAKHFQGRHVLWEIWNEPNGDFWSPKPDAQQYTTLALATCKAIREAEPQATIIG
                     PASSGFPWEFLETFLKSGVLEYLDAVSVHPYRNPKDPPETAATDYLKLRTMIGRYVPP
                     SKKDRIPILSGEWGYSTHKRGVSLETQGAFAARQQLVNLLNGVPLSIWYDWKNDGPDA
                     NENEHNFGTVLPDLQPKPAYVAIKSLTRELSGYRIARRLALPDAKDYVLLCTNQAGAQ
                     KLAAWTLGEPHEVSLEISLKGEDKLAAVSGNGERFMPKLDSGRLLIEIASAPQYVTLG
                     KAALK"
     gene            complement(2164..4764)
                     /locus_tag="VIL39_05870"
     CDS             complement(2164..4764)
                     /locus_tag="VIL39_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEY5480988.1"
                     /translation="MTLYDLIPRDERPGNDDLLGRFLEYTESKRFRLYAAQESAILEV
                     FEEKNVILNTPTGSGKSLVAAAVHFKAVAQGKRSIYTCPIKALVNEKWLGLCREFGPD
                     NVGLSTGDASVNRDAPILCCTAEILANIALREGPQANVQEVIMDEFHYYADRERGGAW
                     QVPLLTLPQARFLLMSATLGDTTFFEEELTRLNGRSTITVASKERPVPLEHAYSELPL
                     ATTLESLVAQGKAPVYVVHFTQLEAAQSAQDFTSINVCTREEKNAIGSVLEGFKFTSP
                     YGPDIRKWLKHGIGLHHAGLLPKYRVLVEQLAQKGLLKVICGTDTLGVGINVPIRTVL
                     FSRLCKYDGEKTGILSARDFHQIGGRAGRKGFDDRGWVVAQAPEHMVENLKLEEKAAR
                     GGKKAVKRKPPERNFVNWDKNTFLRLISAQPERLTSRFRVSHAMLLNVLSRKGDGCRA
                     MQQLIARCHETPKAKKAHVKRAWQLFRSLVERKIIEIVPPGARPSSAAASLNAEGAAA
                     GDGRTPKLRVNIELQDDFSMDQTLSLYLLDTLPLVDPQAPDYPLVLLTLVESILEDPD
                     IILRKQLDKLKSQKMAEMKMEGIEYDARIEELEKLEYPKPNREFVYSTFNAFADKHPW
                     VGQENIRPKSIAREMFESFRSFSDYIRDYELQRAEGVLLRHLNSVYKVLGQTVPDAAK
                     NDTVREMELYLGTMIRQIDSSLLDEWEKMRDPSYQRAEIKEVRPPGAEEAAADVTRDT
                     KAFTATIRNRIFMFLRGLVNRDFEQAVANLSSPQDPDGAPWTADRLQQALDAYHAEHQ
                     YICLDPNARNIRHTYVIPADDQRTWRVQQMLVDPEEHNDWVVEFEVDLARSREAAEPV
                     LRLVRLGSLA"
BASE COUNT          939 a         1578 c         1658 g         1035 t
ORIGIN      
        1 ttccaccgtg cgccaatgct ccggggtgaa tttgggcatg ggcgcggtta cacttcagcc
       61 agacaggaac gctgctcaaa acccagccac gccgggccgc gccactcgcg gcgcacgcgg
      121 cggtatccgg catcctccag cacttccgtc aacgtgccgt gttcagcggc ggtggcgagg
      181 aacagtcgca cggcttcgtc caccgctttg cgcgccgcct ccggggtggg gccagaactg
      241 gccacgtcca gcggcatggc atgggcgatg aattggccgt cctcccgcca gacctgcacc
      301 gtgtaatcaa tctgcatgag agtaacataa ccaatccgcc cctctttgac aacgccagcg
      361 gacgcgccaa cggagaagcc ggctgcgaga ttcacccgtg gcgcctactg agcaaccgct
      421 ttcacctgct acctgagacg ctccagacac catgccagtc aggctttcct tccgcctccc
      481 ttattccgcc ctggaggaga cagcgcattg ccagcgtatt gatggcgtgt tgccagggtg
      541 ttgctccggt attgttacga tggggctacg atgggattac gatgggatta cgatggggtt
      601 acgaagggat tgggcctttt tgccgccccg cgcactccag tggcccagtc cggccgccgc
      661 ttggccgccc acaaacccat gctaaagtcc aaacccaaga gcggggctgc atcacttttc
      721 agtgcatcac ttttcaggtg actattggcg cacatcggtt acacctgctt ggaatgagat
      781 tgatagcgtg cgttcttatg tgtgggctgc cattgcttgg tggcggagcc gaaatcccgg
      841 aaccagtcct gccggcggga gtcggggtga acattcactt tgtaaccggc caggaaaagg
      901 atctcgacct gattgcgggt gcggggttca agtttgtgcg gatggacttt cactgggagg
      961 caattgagac cagcaaaggc gaatacaatt gggctggtta cgatgatctg ctggcaaacc
     1021 tggagaagcg cggcctgcgg gcgatcctca ttcttgatta ctcgcatcac ctttatgagg
     1081 actcggtaac gagcccgcac ccgattaccg gccaggcgca caagacgacc tcctcgccgc
     1141 agcatccggc aagcatcgcc gcttttgccc gctgggcggc agcctcggcc aaacacttcc
     1201 aaggccggca cgtgctgtgg gaaatctgga acgagcccaa cggcgacttc tggagtccca
     1261 agcccgatgc gcagcaatac acgaccctgg ctctggccac gtgcaaagcg atccgcgagg
     1321 cggagccgca ggcgacgatc atcggccccg cctcgtccgg gttcccgtgg gaatttctgg
     1381 agacgtttct caagtccggc gtgctggagt atctcgacgc agtgagcgtg catccgtatc
     1441 gcaacccgaa ggatccgccg gagacggcgg ccaccgatta tctgaagctg cggaccatga
     1501 tcgggcgcta cgtgccgccg tcgaagaagg accgaattcc gattctcagc ggcgagtggg
     1561 gctactcgac gcataagcgc ggcgtgtcgc tggagacgca gggcgcattc gcggcgcggc
     1621 agcagcttgt caacctgctc aacggcgtgc ccctttccat ctggtatgac tggaagaacg
     1681 acggtcccga cgcgaacgag aacgagcaca actttggcac ggtcctgccc gacttgcagc
     1741 ccaaacccgc gtacgtggca attaagagcc tgacccgcga attgtcgggc tatcgcattg
     1801 cacggcgcct cgccttgccc gacgccaagg actacgtcct gctctgcacg aatcaggcgg
     1861 gcgcacaaaa gctggcagcc tggacgctgg gcgaaccgca tgaggtcagc ctcgaaatca
     1921 gtctcaaggg cgaagacaaa ctcgcggcgg tgagcgggaa cggcgaacgc ttcatgccga
     1981 agctcgactc aggccgtctg ttgattgaga tcgcgtctgc accgcagtac gtgaccctgg
     2041 gtaaggctgc cttgaagtaa agaggacggt gagtttgttc cggcagccca ccaggtgact
     2101 ggcgagggcg ccagccacgg cgcccgggac gggcgcgctc cccgtttacg gaatggttac
     2161 ggcttaggcc aggcttccca gcctcaccag ccgcagcacg ggctccgcgg cttcgcggga
     2221 tcgggcgagg tccacttcga actccacgac ccaatcgtta tgctcctccg ggtccaccag
     2281 catctgctgg acccgccagg tgcgctgatc atcggcgggg ataacatagg tgtggcggat
     2341 atttcgggcg ttcgggtcca ggcagatgta ttggtgttcg gcgtggtagg cgtccagcgc
     2401 ttgctggagc cggtcggcgg tccagggcgc gccgtcgggg tcttgcggtg aggagagatt
     2461 ggctacggcc tgctcgaaat ccctgttcac caggccgcgc aggaacatga agatgcggtt
     2521 gcggatggtg gccgtgaaag ccttggtgtc gcgggtaacg tcggcggcgg cttcttcggc
     2581 gccgggcggg cgcacttctt tgatttcagc gcgctggtag ctggggtcgc gcatcttctc
     2641 ccactcgtcg agcaggctgg agtcaatctg gcggatcatg gtgcccaggt agagttccat
     2701 ttcccgcaca gtgtcgttct tggcggcgtc gggaacggtt tggccgagga ccttatagac
     2761 gctgttcagg tgccgcaaca ggacgccttc ggcgcgctgg agttcgtagt cgcggatgta
     2821 atcggagaag gagcggaacg actcgaacat ctcccgcgcg atggatttgg gccggatatt
     2881 ctcctggcct acccacggat gcttgtccgc aaaggcattg aaggtggaat agacgaactc
     2941 ccggttcggc ttggggtatt cgagcttctc cagctcctcg atccgcgcat catattcgat
     3001 cccctccatt ttcatctccg ccatcttctg gctcttgagc ttgtcgagct gcttgcgaag
     3061 gatgatgtcc gggtcttcca ggatggattc cacgagggta agcaggacca ggggataatc
     3121 aggcgcttgc gggtccacca gcgggagggt atccaggaga tacaaggaca gggtctggtc
     3181 catggagaaa tcatcctgca gctcgatgtt gacgcgcagc ttcggagtgc ggccgtcccc
     3241 ggccgcagca ccttccgcat tcagacttgc tgcggccgag gacggccgcg ctcccggggg
     3301 cacaatctca atgatcttgc gctcgaccag cgagcggaag agttgccagg cgcgtttgac
     3361 gtgggccttc ttcgctttgg gcgtttcgtg gcagcgggcg atcaactgct gcatggcgcg
     3421 gcagccgtcg cctttccggc tcagcacgtt caacagcatc gcgtgcgaca cgcggaaccg
     3481 cgaggtcagg cgttccggct gggcggagat caggcggagg aaagtgttct tgtcccagtt
     3541 gacgaagttc ctctccggcg gcttgcgttt gacggccttc ttgccgcccc gcgcagcctt
     3601 ctcttcgagc ttgaggttct cgaccatgtg ttcgggcgcc tgggccacca cccagccgcg
     3661 gtcgtcgaac cctttgcgcc cggcgcgccc gccgatctgg tgaaaatccc gcgcgctcag
     3721 gatgccggtc ttctcgccgt catacttgca caggcgcgag aacagcacgg tgcggatggg
     3781 gacgttgatg ccgacgccga gagtgtcggt gccgcagatg actttgagca ggcccttctg
     3841 cgccagttgc tcgaccagca cccggtactt cggcagcagc ccggcatggt gcagcccgat
     3901 gccgtgctta agccacttcc tgatgtccgg cccgtagggg ctggtgaact tgaagccttc
     3961 gagcacgctg cctatggcgt tcttctcctc gcgcgtgcag acgttgatgc tggtgaaatc
     4021 ctgcgcgctc tgggccgcct ccagttgtgt gaaatggacg acatagacgg gggccttgcc
     4081 ttgcgccacc aggctttcga gcgtggtggc cagcggcagt tcggaatagg cgtgctccag
     4141 cggcaccggc cgctccttgg acgcgacggt gatggtcgaa cgcccgttaa gccgggtcag
     4201 ctcttcctcg aagaaagtcg tatcgcccag cgtggcggac atcaatagga accgggcttg
     4261 cggcagggtc agcagtggca cctgccaggc cccgccacgc tcgcggtcgg cgtagtaatg
     4321 gaactcgtcc ataatgacct cctgcacgtt ggcctgcggc ccttcgcgga gcgctatgtt
     4381 ggcgaggatt tcggccgtgc aacagaggat cggcgcatcg cgattcaccg aggcatcacc
     4441 ggtgctcagg ccgacattgt cggggccgaa ctcgcggcat aggccgagcc acttctcgtt
     4501 gaccagggcc ttgatcgggc aggtatagat ggaacgtttg ccctgcgcga ccgccttgaa
     4561 atgcaccgcg gcggccacga gcgatttgcc cgaaccggtg ggcgtgttga ggatgacatt
     4621 cttctcctcg aacacctcca ggatggccga ttcctgcgcg gcataaagcc ggaagcgctt
     4681 gctctcggta tactcgagga aacgcccgag caggtcatca ttgccggggc gttcatcccg
     4741 ggggattaga tcgtagagtg tcaaagcagc ttccccaacc gttgaagttc cattaggaac
     4801 tcgatgtctg gcggagcaac gcggccttcc agcgattcat ttcagcatgc gtccgaaact
     4861 ttcgcgcctt gaaccgaggc aaaggagggc ctggcggagg cgccaccact ctggggtgct
     4921 cggcccacag ccacatcttg gctttgtagt catcggttac tttcatcttg ggcggcctga
     4981 gaaccagcat cgtctgtttc cgcagccatc cctaatcttg tccaaacccg ggctgattgc
     5041 acgcgctttc tagcgggatt tgcgaccagg cagcgccaat tcatgaaggc cctttggcgc
     5101 aaaggaggcg cttcattctg ggctgctggc tgcggggtga ttcgggctca ggggctagcg
     5161 ccccatgggc tgagggttct ggaagcggca agagccccgc agggtggagt
//
LOCUS       DATCGS010000288         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_323499, whole genome shotgun sequence.
ACCESSION   DATCGS010000288 DATCGS010000000
VERSION     DATCGS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323499"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="VIL39_05875"
     CDS             complement(<1..1833)
                     /locus_tag="VIL39_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5480989.1"
                     /translation="MQDLLFLAFLMTVSTATPQTPASIQRTTQTSVPPVAKKVPKEIV
                     TNGDKRVDDYFWLREKTNAEVLAYLEAENAYGEAVTRPQRPFRERLYAEMLGHLQETD
                     SSAPVRRGEFFYYSRTEKGKNYPIFCRKYGSLKAPEEIILDQNALAEGHKFFNLGVFE
                     PSDDNLLLAYTTDSTGYRQYTLHIKDLHSGKLLPDQFERVDEVVWVTDNKTLFFVTEN
                     EVTKRQDEFHRHTLGSASAERVYFEPDELFDLSVDRSRDRAFVFLTAESKLSTEVRFL
                     PAGQPDAPLRLVQPREPDHKYFVTHRRGQFYIRTNDKAKNYRVATTPVEHPGKAAWKE
                     FIPHNRAVKIDDFDLFAGHAVVSERENGLERIRIINLENQQSHAIELPEPAYDLAVDT
                     NPEFDTTVLRYRYQSLVTPGSTYDYDMNSRRRTLVKQTKVPGYKPASYVSERIFATAS
                     DGTKIPCSVVYKKGMQRNGRNPLFLYAYGSYGYSVSATFSYSRLALLDRGMIYVMAHI
                     RGGGEMGEVWRDQGRMMLKRNTFTDFIACAEHLIGEKYTSADRIAIQGASAGGLLMGA
                     VVNMRPGLFRVVIAQVPFVDVLNTMLDASLPLTTSEYIEWGNPNE"
     gene            complement(2677..3315)
                     /locus_tag="VIL39_05880"
     CDS             complement(2677..3315)
                     /locus_tag="VIL39_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480990.1"
                     /translation="MRGRPGMGGRRRTGRSADQQEGSAAFTYVMKMWEVLSDEERLAW
                     NVQGSTRRSHGINYFKTVNLRRARRGEELTRLPPSPKPYEAKPVLKRLVIRNRGDRIT
                     LMLEFLRVPSVPTTVWGSRPCNRGLARPDKCPRLGWLIVSPEVMIDITVLYFKKHGGY
                     IQQQGMELVGKRVFIRTRQEMDDGANLFEEVQAVIPPPERPRRTRQKPPFPS"
     gene            complement(3488..>4207)
                     /locus_tag="VIL39_05885"
     CDS             complement(3488..>4207)
                     /locus_tag="VIL39_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5480991.1"
                     /translation="LIPDSALDGGRKEFIKRLADPEQKAKIVAEMKQSLSRKGRDDFS
                     YAVIAAYKHDKSLDGLSIAEAAKAKRGSDSLGDQIEMILEIEKEGGASGVFHGMSEDD
                     LKTFMRHPNTMIACDSGLRKLGEGMPHPRGYGNNAHVLARYVRELKVLRLEDAIRKMT
                     SLPANTFHFKGRGELREGNWADIVVFDPDKIQDNATYKDPHHYATGIPYVLVNGVLVI
                     KNGQHTGAKAGQGLRHVCTPR"
BASE COUNT          718 a         1264 c         1327 g          898 t
ORIGIN      
        1 ttcgttcggg ttgccccact cgatatattc gctggtggtc agcggcaggg aggcatccag
       61 catcgtgttc agcacatcca caaatggcac ctgggcgatg accacgcgga agaggccggg
      121 ccgcatattg accaccgcgc ccataagcaa gcctcccgcg ctcgcgccct ggatcgcgat
      181 ccggtcggcg gaggtgtatt tctcgcctat cagatgctcg gcgcacgcaa tgaaatcggt
      241 gaacgtattg cgcttgagca tcatccggcc ctggtcgcgc cagacctcgc ccatctcccc
      301 gccgccgcga atgtgcgcca tcacataaat catgccccgg tccagcaagg cgagccggga
      361 ataggaaaag gtcgccgaga cggaatatcc gtaggaacca taagcgtaca ggaacagcgg
      421 gttgcgtccg ttccgctgca tgcccttctt atagaccacc gagcacggga ttttcgtgcc
      481 gtccgaagcg gtggcgaaga tgcgttcgga gacgtagctg gcgggcttat agccgggcac
      541 cttggtttgt ttcaccagcg tgcgccggcg gctgttcatg tcgtaatcat aggtagagcc
      601 gggcgtgacg agggattggt agcggtagcg caacacggtg gtgtcgaact ccgggttggt
      661 gtccaccgcc aagtcgtagg ccggttccgg gagttcaatc gcgtgcgatt gctggttctc
      721 caggttgatg atgcgaatcc gttcgaggcc attctcgcgt tcggacacca ccgcatggcc
      781 ggcgaacagg tcaaagtcgt caatcttcac tgcccggttg tgggggatga attctttcca
      841 ggccgctttg ccggggtgct ccaccggggt agtggcaacg cggtagttct tggctttatc
      901 attggtgcgg atgtagaact ggccgcgccg atgggtgacg aagtatttgt ggtccggctc
      961 acgcggctgc accaggcgca gtggtgcgtc aggctggccc gccggcagga accggacctc
     1021 ggtgctgagc ttgctctcgg cggtgaggaa aacaaacgcc cggtcccggg agcggtccac
     1081 ggagaggtcg aacagctcgt ccggctcgaa atagacccgc tccgccgaag cgctgccgag
     1141 cgtgtgccga tggaactcgt cctggcgctt ggtgacctcg ttctcggtga cgaagaagag
     1201 ggtcttgttg tccgtgaccc aaaccacctc gtccacccgc tcgaattggt ccggcaacag
     1261 cttgcccgag tggaggtctt tgatgtggag cgtgtattgg cggtagccgg tgctgtcggt
     1321 cgtataggcg agcaggaggt tgtcatcgct gggctcgaac acgccgaggt tgaagaattt
     1381 gtgcccttcg gccagggcgt tctggtcgag gatgatttcc tccggagcct tgaggctgcc
     1441 gtacttgcgg caaaagatcg gatagttctt tcccttctcc gtgcgcgagt aatagaagaa
     1501 ttcgccgcgg cgaaccgggg ccgaggaatc cgtttcctgg aggtggccca gcatttcggc
     1561 gtagagccgc tcgcgaaacg gccgctgggg gcgggtgaca gcctcgccgt acgcattctc
     1621 ggcttcgaga taggcgagga cctcggcatt ggtcttttcg cgcagccaga agtaatcatc
     1681 cacccgcttg tcgccgttgg tgacgatctc cttcggcacc ttttttgcca cgggcgggac
     1741 ggacgtttgc gtcgtgcgct ggattgaggc aggcgtttgg ggcgtagcgg tggaaacggt
     1801 cataagaaag gccaggaata acaaatcttg catgcgggtt cggcacggtg aggctgcgga
     1861 attgcgccgc cgccccacct tgctgagcag ggctctgaag ctgtggtttc gaagtccggt
     1921 caccatctgg taaggctagg gccggagccg gtctttgcaa acaagatttg attctcccgg
     1981 cgaagcgaaa ggggcaccca ttagccatcg catctcacac ctgagtagcc gccggcggca
     2041 gggcgtgttg cccaagcggt tcccaggcag ggcacgctgg actgtcatat ctcgatttgc
     2101 tcgcaggctg cgaagatcta acgcagagac gcgaagagcg cagcgcggca aagccacaat
     2161 cgaatggaag aaggcagaat catggccgga cagaatcata tttgacggac catgcggctc
     2221 cgccttttgt ggtcccgcca gcaataatac catctttgtt ccgcatgatt ctgtcctgcc
     2281 atgattctgt cggtctcatg cgggacaagg aatgctcgca gcttgcgaac aacttcgact
     2341 attgccgtgc agagattcgc agagggtgct tcttctttgc ttttctccgc gctcttcgcg
     2401 tctctgcgtt aaagctggtt tggttttggt tacgattttg ccgcgctgtg gacagcgcgc
     2461 cctctgagtg gtcagtcctt gattcggcct ccctgagagg caccgagcgg cgaatgggta
     2521 tcttctggaa tggtttcggc cttcgggttt cggagcgctc cggtctcctc gccgccgcaa
     2581 gccacttgta cccgcggtgg tagcgtgctg ctctgccgca gcgagcgtga tgccacgaag
     2641 gagttacgac ggggcttcga aggggttacg aaggagttac gaagggaatg ggggtttttg
     2701 gcgcgtccgc ctcggccttt caggcggagg aatgaccgcc tggacttcct caaaaaggtt
     2761 tgcaccgtcg tccatctcct gccgggtgcg gatgaaaacc cgctttccca caagctccat
     2821 cccttgctgc tggatgtacc cgccgtgctt tttgaagtag agtactgtga tgtcgatcat
     2881 cacctccggc gacactatca gccagcccag gcgggggcac ttgtcgggcc tcgccaggcc
     2941 gcggttgcag ggccgcgaac cccagacggt cgtcggcacg gatggcacgc ggaggaattc
     3001 cagcatcaag gtaatccggt cgccccggtt gcggatgacc agacgcttca ggaccggctt
     3061 ggcctcgtaa ggcttgggcg atgggggtaa ccgggtcaac tcctccccgc gacgcgcccg
     3121 ccggaggttg accgtcttga agtagttgat cccgtgcgag cgccgggtgc tgccctggac
     3181 gttccacgcc aggcgctcct cgtcgctcag cacttcccac atcttcataa cgtaggtaaa
     3241 cgccgcgctc ccttcctgct ggtcggcgct ccggcccgtt cgccggcggc cacccatgcc
     3301 cggcctacca cgcataggtc cgcctcccgg tggcctgctc gcccaccctg ccctgctgcc
     3361 caatgttggc tgttctcata tggtcatctt ccgagcctcg gtcgcggcac ccacgcgccg
     3421 ccaacaaccc cgtgtcatcc gtctgccaaa ctctcgccgg atactatcac acggcccctc
     3481 ggcggcgcta cctcggagtg caaacgtgcc gcagcccctg gccggctttc gcgcccgtgt
     3541 gctggccgtt cttaatcacc aggacgccgt tcacgagcac atacggaatg ccggtggcgt
     3601 aatggtgcgg gtccttgtag gtggcgttgt cctgaatctt gtcggggtcg aagaccacga
     3661 tgtcggccca gttgccctcg cgcagctcgc cgcggccctt gaagtggaag gtgttcgccg
     3721 gcaggctggt catcttgcgg atggcatcct ccaggcgcag cactttcagc tcgcggacat
     3781 aacgagccag cacatgcgcg ttgttgccgt aaccgcgcgg gtgtggcatg ccctcgccca
     3841 gcttccgcaa gccgctgtcg caggcgatca tggtgttcgg gtgccgcatg aaggtcttta
     3901 gatcgtcctc gctcatgccg tggaagacgc cactggcgcc accctccttt tctatttcca
     3961 ggatcatctc gatctggtcg ccgagcgaat ccgagcctcg cttggccttg gccgcctcgg
     4021 caatgctgag gccgtcgaga gacttgtcgt gtttgtaggc ggcgatgacg gcgtagctga
     4081 agtcgtctcg ccccttgcgg ctgaggctct gcttcatctc ggcgacgatc ttggctttct
     4141 gctccgggtc ggcgagacgc ttgatgaact ccttgcggcc gccgtccagc gcggagtcgg
     4201 ggatcaa
//
LOCUS       DATCGS010000289         4313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_24149, whole genome shotgun sequence.
ACCESSION   DATCGS010000289 DATCGS010000000
VERSION     DATCGS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24149"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VIL39_05890"
     CDS             <1..1377
                     /locus_tag="VIL39_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5480992.1"
                     /translation="SIIHQVFPLMEINKQIYGLNVDQLLADNRKQIDSIRQTTEFADL
                     INRTIVSCRGSHFWIAAPSRSAYHEGFVEEAAYELADSYAQYVEMSRAGNSTELPLLY
                     FNGSYYTAQDFTCGGTTYPKGLKVRTCNGQPPDALVQALSRSGVALDWDYDRNKYYTT
                     RFYKYAPAAKSNSAVTLELAGSNGASMTMEVSEAKRAQWQERPHASARPQVALVNSNI
                     LYIRLPAMDPKQLPFYRKELGKYRGQAIRKVVIDIRNNGGGSDNVWNELLSLLVKEKL
                     MLSYALAAKASDINTDYLARHGFGKGLAKNGKVEHITFLNNQELRVLRTSAPIVPQAD
                     SLKLGCSIFVLSDHVYSSAGSLMDICKQSRQLVSVGLPNSQILGVGIDPFAFSLPNSK
                     IVFLLEPVVDLTGARTAKDTHHNDVEVRIKPTLDQLLDYYGTDAALPLEERLNQHDPF
                     FQRVLELD"
     gene            complement(1622..2332)
                     /locus_tag="VIL39_05895"
     CDS             complement(1622..2332)
                     /locus_tag="VIL39_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5480993.1"
                     /translation="MKTRLTFSAGRACRLAFFPPARLKPTWPGKVAAVMAVLLCAGSG
                     FAGSLAVTITSEGLTWPFGDSGNGYTVLSGALPHWSFPAGVVCTSQPYVGTANSNCVS
                     VRIPYFENQGSLEPLYITVTETGFGPNPTADTLTMTFSASGFSCIQTGMINGITMGSI
                     GPAPGTVSLPVSGPASGFSMSESLAVFSDTPYASGSFSAELFLYHNPVPEPSTLGLAG
                     LGLAGLLAAQRTRSGRHA"
     gene            2918..3547
                     /locus_tag="VIL39_05900"
     CDS             2918..3547
                     /locus_tag="VIL39_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5480994.1"
                     /translation="METKSMSMSCQSSGFSNLDIGSPKLPGCAKLVEAGWDLIAGGAD
                     IWEQSDQFHFVCKQISGDFDVAVRVESFTPAHLYSKAGLMIRANLNADSAHVMFLVFA
                     DNSPRNNNLGAYEMQFRSAAGIDCQAIYPTVRPPAAPEFPAAYPNSWLRIQRRGDRFS
                     AFASTDGKTWRLYSVQTMTLPRRVYVGPALTSHDPQKTAKACFRNYTGG"
     gene            complement(3486..>4313)
                     /locus_tag="VIL39_05905"
     CDS             complement(3486..>4313)
                     /locus_tag="VIL39_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEY5480995.1"
                     /translation="THLWDGQWFGRGITDDGVVFGVSKDKEGRIFLNPQSWAILSGAA
                     DEAQRGLMLQAVAEQLETPYGVMILAPAFTAMRDDVGRVTQKFPGSAENGSVYNHAAV
                     FYIYSLYSIGERDRAFRLIRQMLPGPDIADYQQRGQLPVFIPNYYRGACSQHPRTAGR
                     SSQLFNTGTVAWLYRCLVEGLFGLRGDKHGLVIQPQLPSHWNRAKVTRQFRGAIFEVE
                     MRRESGISNAQVVVDGQPLPANRLTDIRVGKTYKVEVVIPRCSYESRPLPSFAGHAT"
BASE COUNT          903 a         1293 c         1272 g          845 t
ORIGIN      
        1 tccatcattc accaggtgtt cccgctaatg gagatcaata aacagatcta tggcctgaac
       61 gtggaccagt tgctggcgga caaccgcaag cagattgata gcatccggca gacgacggag
      121 tttgcggacc tcatcaaccg cactattgtc agttgccgcg gcagccactt ctggatcgcg
      181 gctccgtcca ggagcgctta ccacgagggt tttgtggaag aggccgctta tgagctggcg
      241 gacagctacg cgcagtatgt ggaaatgagc cgcgccggaa acagcactga gctgccgctg
      301 ctctacttca atggcagcta ttacacggcg caggatttca cctgcggcgg caccacctac
      361 cccaagggcc tgaaggtccg gacctgcaac gggcaaccgc cggacgcgtt ggtgcaggcg
      421 ctcagccgct cgggcgtggc gttggattgg gattacgacc ggaacaagta ttacaccacc
      481 cgcttctaca aatacgcgcc cgcagccaag agcaattcgg cggtcaccct ggagttggcg
      541 ggcagcaacg gcgcttccat gaccatggaa gtcagcgagg ccaagcgcgc ccaatggcag
      601 gagcggccgc acgcctcggc ccgcccgcaa gtcgccctgg tcaatagcaa catcctctac
      661 atccgactcc ccgcgatgga ccccaagcag ctcccgtttt accgaaagga gttaggcaag
      721 tatcgcggcc aagccattcg caaggttgtc atcgacatcc ggaacaacgg cggcggcagt
      781 gacaatgtct ggaacgaact gctctcgctt ctcgtaaagg agaagctgat gttgtcctac
      841 gccctggcag ccaaagcgtc ggatatcaat acggactacc tggcacggca tggttttggc
      901 aaggggctgg ccaagaacgg caaagtcgag cacatcacct ttctgaataa tcaagagctc
      961 cgcgtgctcc ggacctccgc gcccatcgtg ccccaggccg actcgctcaa gctcggttgc
     1021 agcatctttg tcctgtcgga tcatgtctat tcctctgccg gcagcctgat ggacatctgc
     1081 aagcagagtc ggcaactggt ttcggtgggt ttgcccaaca gccagattct gggcgtgggc
     1141 atcgacccct ttgccttctc gctgccgaac tccaagatcg tattcttgct cgagccggtg
     1201 gtggatctaa ccggcgcccg gaccgccaag gacacgcacc acaacgatgt ggaggtgcgg
     1261 atcaaaccca cgctcgacca gttgctcgac tattacggca ccgacgccgc cttgcccctc
     1321 gaggagcggc tcaaccagca cgacccgttc ttccagcggg ttctggaact ggattagctg
     1381 ggggccatcg gcgaagaccg ttacggtatg ggtgccgagc gccaggtcca cacccatgct
     1441 gagggcggca tcgtggtcgc gcagcgcttg ctcttggctg ctcacttttg acactggccc
     1501 cccggccccg cagcgtgagc tgagggcccc gtcaccgggc tggcatgtcc ggcatctcta
     1561 ctgtccaggc cgaccaatcc caaccgctgt tgatgcgaag gctaaaggct gaaggctaaa
     1621 gtcaggcgtg gcgaccactg cgagtcctct gagcggccaa caatcccgcc aaccccaaac
     1681 ccgccaaccc caacgtggac ggctcaggaa cgggattatg gtaaaggaag agttcggcgc
     1741 tgaagctgcc cgaggcgtac ggcgtgtcgc tgaacacggc caggctctcc gacatgctga
     1801 acccggaggc cgggccactg actggcaaac tcacggtgcc cggcgcagga ccgatcgagc
     1861 ccatcgttat gccattaatc attcccgtct gaatgcaact gaaccccgaa gccgagaaag
     1921 tcattgtcaa agtatctgcc gttgggtttg ggccgaagcc ggtctcggtg accgtgatat
     1981 acaaaggctc caggctgcct tggttctcga aatacggaat cctcacactg acgcaattgc
     2041 tgttagccgt gcctacatac ggctgggaag tgcacacgac gcctgcggga aacgaccaat
     2101 gggggagcgc gccgctcaaa accgtgtaac cattaccgct atcgccgaag ggccacgtca
     2161 gtccctccga ggtgatggtg accgccaggc tgccggcgaa cccagaaccg gcacagagga
     2221 gcacagccat gacagccgcg accttgccgg gccatgtagg tttcagcctc gccggcggga
     2281 aaaatgccag ccgacaagct ctgcccgcgg agaacgtcag gcgtgttttc atagactatg
     2341 ttgcttaagg tacgccccgc ctgaataacc attgttgctt cacgggtcaa ggttttcagt
     2401 tcgcgcgctg cgggaccgat ttccaactgt cggttggggg atggggctcc gatgcgaatg
     2461 ggacagctcc tgcggcccag aggtagggcg ccatttcggc ggcgacaagg gcgcccattt
     2521 tgcggatgtc ataaggtaaa ggggggcaaa ccgagccagt gtcaaaagtg aggaccgaca
     2581 cccggcacgg aatcaagtgg cgggcggcgg aatgggcacg cgaggcgggg cggtttgcag
     2641 caccaacgaa attcgaccac atgattctgc attctgcatg cttccttctg acttccccct
     2701 tggtggtgct cgtgtgttcg tcctacaaga tttgaacgcc agcgcagaat aggaggtgtt
     2761 ccttgccggg gaggcacctc gatgagccaa tctagggtct gacccgttcc aggatcggcc
     2821 acgagcgacc tcagacgcta gttggggatg aatgagggtt gtcgagcctc cgccatcacg
     2881 cttacagtgc ccctgcgaca aagccatttt gaactatatg gaaactaaat caatgtccat
     2941 gagctgccaa tcctcggggt tttctaatct cgacattggc tcaccgaaac tgcccggttg
     3001 cgccaagctg gttgaggccg gctgggacct catcgccggc ggagcggata tttgggaaca
     3061 atcagaccag tttcattttg tgtgcaaaca gatttccggc gacttcgacg ttgccgtccg
     3121 cgtggagagc ttcacgccgg cacatcttta ttccaaggcg ggtctcatga ttcgggcgaa
     3181 cttgaacgca gacagcgccc acgtgatgtt cttggtcttt gccgacaatt ccccacggaa
     3241 caataatctg ggagcttatg aaatgcagtt tcgctcggcg gcaggaatcg attgccaggc
     3301 gatttatccg acagtaaggc ctcctgccgc gccagaattt cccgctgcct accccaattc
     3361 atggctgcgg atacaacgtc ggggggatcg gttttcggcc tttgcctcca cggatggaaa
     3421 gacctggcgg ttgtattcgg tccaaacgat gacgcttccc cgccgagtct atgttggtcc
     3481 cgcgctcacg tcgcatgacc cgcaaaagac ggcaaaggcc tgctttcgta actacaccgg
     3541 gggatgacta cctctacttt atatgttttg cccacccgaa tgtcggtgag ccggttggcc
     3601 ggcaatggtt ggccgtcaac gaccacctgg gcgttagaaa tgccgctttc acggcgcatt
     3661 tcaacctcga agatagcgcc gcggaattgt cgggtgacct tggcccggtt ccaatgactg
     3721 ggcagttgcg gctgaatgac cagcccatgc ttgtcgcctc ttaggccaaa caatccttca
     3781 accaggcagc gatacagcca ggcgacagtg ccggtgttga acaattggct ggagcggccc
     3841 gctgttcgcg gatgctggga acaggcgccg cggtagtaat tggggataaa caccggcaat
     3901 tggccccgtt gttggtagtc tgcgatatcc gggccgggga gcatctgacg gatcaggcgg
     3961 aaggctcggt cccgctcgcc aatgctgtag aggctgtaga tgtagaacac cgcggcgtgg
     4021 ttataaaccg aaccgttctc ggccgaaccg gggaacttct gcgtgacgcg cccaacatcg
     4081 tcgcgcatgg cggtgaaagc cggggccagg atcatgacgc cgtaaggggt ctccaactgc
     4141 tcggccaccg cctggagcat cagtccgcgc tgcgcttcat cggcggcccc gctcagaatc
     4201 gcccagcttt ggggattcag aaagatgcgg ccttctttgt ctttgctgac accgaaaacc
     4261 acaccgtcat cggtgatgcc gcggccaaac cattggccgt cccagagatg ggt
//
LOCUS       DATCGS010000290         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_670074, whole genome shotgun sequence.
ACCESSION   DATCGS010000290 DATCGS010000000
VERSION     DATCGS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670074"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /gene="dctP"
                     /locus_tag="VIL39_05910"
     CDS             complement(<1..764)
                     /gene="dctP"
                     /locus_tag="VIL39_05910"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5480996.1"
                     /translation="MFPLIVLILGLALTAPGLHAADKVIKFKLGTLAPAGTSYHKSLQ
                     AMGEKWRKASDGTVQLMIFPGGTQGSEADMVGLMQTGNLDAGLLTTGGLSEIDSAALA
                     MQVMPMFFRNLDELDYVAEKLRPQLDARLLAKGYVVLFWSDSGWVRFFSKSPVLHPDD
                     LRKLKVFSGADSAAAYDVWKATGFSPVSLDATGIPQGLLSGTISAVPTVPIYALAGQL
                     DSQAKYMLELNWGPLVGAAVVHKKTWDRVPAAAREA"
     gene            complement(897..1769)
                     /locus_tag="VIL39_05915"
     CDS             complement(897..1769)
                     /locus_tag="VIL39_05915"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEY5480997.1"
                     /translation="MAVNKLGNALAGSGTTFASDDDPELVKAAVPFSLKLMEGLLNEN
                     PRHEGLLLAASSGFTEYAYAFVQEEADETEDKDLAAAEEMRGRARRLYLRGRNYGLRG
                     LEVRHKGFEKALRADPKQAVRAATMKDVPLLYWTAVSWAGAVSLSKDNPDLLADMPIV
                     EAMMDRALALDETFGDGAIHTFLITYEMSRSGGTGDPAARSRQHFERALELSKGLQAG
                     PMISFAEAVCVQKQDLKQFETLLQQALALNPEVKPEWRLVNLVMQRRAKWLQSRTDQL
                     FLSSSPAGSKKSEP"
     gene            complement(1922..>2725)
                     /locus_tag="VIL39_05920"
     CDS             complement(1922..>2725)
                     /locus_tag="VIL39_05920"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY5480998.1"
                     /translation="RVANRIAGARFTLDGVEYKLAANSGPNHIHGVFDKVVWEAKALP
                     PGKGRAAVQLTYLSKDGEDGYPGNVTVKLTYTLTDENELRLDYEAVTDRATPINLTSH
                     AYFNLAGGGDVLDHELWLAAAHYTPADDALILTGQIASVAGTPLDFTKPTRIGARIAQ
                     FYPKPGGYDHNFVLDGEPGRRAAGPLGNGKPLVLAARVREPVSGRAMEVRTTEPGVQL
                     YTGNHLKHAALCLETQHYPDSVNHPGFPSTILRPGPAFSSTTTFAFSAK"
BASE COUNT          500 a          884 c          809 g          532 t
ORIGIN      
        1 atcgcttcgc gggcagccgc cggcacgcgg tcccacgtct tcttgtgcac gaccgccgcg
       61 cctaccaggg gcccccagtt caactccagc atgtacttgg cctggctatc caactgccct
      121 gccagcgcat aaatgggcac cgttggaacc gcgctgatgg tgcccgataa caggccctgc
      181 gggatgcccg ttgcgtccag ggataccggg ctgaacccgg tggctttcca cacgtcatac
      241 gcggcggcgc tgtccgcccc ggagaaaacc ttcagcttcc gcaagtcatc cggatgcaac
      301 accggcgact tggagaagaa ccgcacccag ccggagtcac tccagaacag gacgacgtat
      361 cccttggcca gcaggcgcgc atcgagttgc ggacgcaact tctccgccac gtagtccagc
      421 tcatccagat tgcggaagaa catcggcatc acttgcatgg ccagagcggc ggagtctatt
      481 tccgagagcc caccggtcgt gagcaaaccg gcatccagat tgcccgtctg catcaggccc
      541 accatgtccg cctcgcttcc ctgcgtgccg ccggggaaaa tcatcagttg cacggtgccg
      601 tccgaggcct tgcgccactt ctcgcccatg gcctgcaggc tcttgtggta ggaggtgccc
      661 gcgggcgcca gggtgccgag cttgaacttg atgactttat cggcggcgtg caggcctggc
      721 gcggtcaagg ccaagccgag gatcaacaca atcagaggga acaaaccaca gatggacaca
      781 gatggacaca gatgatccaa aggggcgtga ctctcacctg ctggctgaag cgctgcttgg
      841 tccgatttgc tgttgtatct gtgtctatct gtgttcatct gtggttggac tgctttttag
      901 ggttcagact tcttgctccc ggctggactg gaactcaaga aaagctggtc ggtgcgcgac
      961 tggagccatt tggcccggcg ctgcatgacg aggttgacca gccgccactc cggtttgacc
     1021 tcggggttga gcgccagcgc ctgctggagc aaggtctcga actgcttcag gtcctgtttc
     1081 tgcacgcaca ccgcctcggc gaaggagatc atcggccccg cctgcaagcc cttgctgagc
     1141 tccaacgccc gttcgaagtg ctgccgcgac cgcgcggcgg gatctcccgt cccccccgac
     1201 cggctcattt cgtaggtgat caggaatgtg tgaatggcgc cgtcgccgaa cgtctcgtcc
     1261 agtgccagcg cgcgatccat catcgcctcc acgatcggca tgtcggctaa caaatcgggg
     1321 ttatcttttg aaagcgagac ggcccccgcc caggaaaccg ccgtccagta gagcagcggc
     1381 acatctttca tggtggccgc gcgcaccgcc tgtttcgggt cggcccggag cgccttctca
     1441 aagcccttgt gcctgacctc caacccgcgc aagccatagt tccgcccgcg cagatacaaa
     1501 cgccgggcgc gccctctcat ctcctccgcc gcggccaggt ccttgtcctc cgtctcgtcc
     1561 gcttcttcct ggacaaacgc ataggcatat tccgtaaagc cgctggaggc ggccagcaac
     1621 agcccttcgt ggcgcggatt ctcgttcagc aggccctcca tgagcttgag gctgaacggc
     1681 accgcggcct tcaccagttc tgggtcatca tcggacgcaa aggtcgtccc gctgcccgcc
     1741 aacgcattgc cgagtttgtt cacagccatg cgcttgacgg agcagcccga gccaaccacg
     1801 gccagaccca ccatcatcaa ccaaacaaat tggtttatcg cacccacaga catcggttca
     1861 gatttcgagt tgtagtatat gccagtccgg gcgcgaagtg ccaatcacta agcactccac
     1921 ttcatttcgc cgaaaaggca aaggtggtgg tgctgctgaa ggccgggccg gggcggagaa
     1981 tcgtggaggg gaagccgggg tgattcacgg agtcgggata atgttgcgtt tccaggcaga
     2041 gagctgcgtg cttcaggtga ttgccggtgt aaagctgcac tcccggttcc gtcgtgcgca
     2101 cttccatcgc acgcccactc accggctccc ggacacgtgc tgccagcacg agcggcttcc
     2161 cgttcccgag agggcccgcc gcacgccgcc cgggttcgcc gtccaagaca aagttgtggt
     2221 cgtagccgcc cggtttcgga tagaactgcg cgatgcgcgc gccgatgcgg gtcggtttgg
     2281 tgaaatccag cggtgttccc gccacggacg cgatttggcc cgtgagaatc aaggcgtcgt
     2341 ccgcgggagt gtaatgagca gccgccagcc acaattcgtg gtcgagcacg tcgccgccgc
     2401 cggcgaggtt gaagtaggcg tggctggtca gattgatcgg cgtggcccgg tcggtgaccg
     2461 cttcataatc caagcgcaat tcgttctcgt cggtaagcgt gtaagtcagt ttcaccgtca
     2521 cgttgcccgg gtaaccgtcc tccccgtcct tgctcagata agtgagttgg acggcagctc
     2581 gccccttgcc gggaggaagc gctttggcct cccagacgac cttgtcaaat acaccgtgaa
     2641 tgtggttagg cccgctattg gcagccagtt tgtattccac gccgtccagt gtaaaccgcg
     2701 cgccggcgat gcgattggcc acccg
//
LOCUS       DATCGS010000291         3678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_175354, whole genome shotgun sequence.
ACCESSION   DATCGS010000291 DATCGS010000000
VERSION     DATCGS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175354"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="cheB"
                     /locus_tag="VIL39_05925"
     CDS             <1..816
                     /gene="cheB"
                     /locus_tag="VIL39_05925"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY5480999.1"
                     /translation="IMASKPCAIVVVTANVTGQSAKVFEAMGAGALDAVNTPVIGRTG
                     GGEGARALLGKIETIRRLIGSATARNPWAPAQRVKRHLTQHRNHLIVIGASAGGPTAL
                     AKVLAHLPTDFPAPVIIVQHVDSHFVPGLANWLSGQTPLQVRLAREGDRPQAGTALVA
                     GQDNHLVFASSTRLVYVRHPADCFYRPSIDLFFKSADQFWPGDVVGVLLTGMGRDGAE
                     GLRALHSRGHHTIAQDQLSSAVYGMPGAAAELRAATEILSLDKIGPRLTNIVA"
     gene            830..3391
                     /locus_tag="VIL39_05930"
     CDS             830..3391
                     /locus_tag="VIL39_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481000.1"
                     /translation="MAEEHTNQIMNLDNQIVNLDQERPPHPPDYHVMVLLVDDQAMVC
                     EAVRRALANQTDIDFHYCADAREALAIANQIKPTVILQDLVMPGVDGLSLVKQFRANP
                     ATRDTPIIVLSTNENPQVKGQAFALGANDYLVKLPDKIELIARIRHHSKSYLNLLQRD
                     AAYRALRESQQQLIESNAALISLNQKLEEATLAKSQFLANMSHEIRTPMNGIIGMTAL
                     LLDTALTDEQRDYVEATRSSGDALLTIINDILDFSKIESGKVELEHHPFELHTCIEEA
                     MDLLAPKAAEKNLDLACLVDDAIPKILVGDVTRLRQVLVNLIGNAVKFTHQGEVVVEV
                     TSSGRTVRNIPFGHEQDTEFIRNPNEWLLHFSVRDTGIGIPVDRQSRLFKSFQQVDAS
                     TTRHYGGTGLGLAICKRLVKLLGGKIWIESDAGKGSTFHFTISVRAPASTTRPAWQNP
                     QPQLAGKRLLVIEDNATNRRIITQRGQQWGMTVETAVNRREALPLLAQGDRFDSVILD
                     LELPDKDGLALAEEIRNQPSGRYVPLLLLSSKRPSGDDAHPLPAGVSVSIHKPIRPAQ
                     LLDALCRALSVRVQREKKTPVTPTLDPDLARRLPLHLLLADDNPINQKVGLTILRKLG
                     YRADIANNGLEVLKALEQKAYDILFLDVQMPEMDGLEAARQICQRWPAEKRPRIIAMT
                     GNALMGDREKCLQAGMDDYISKPVRIADLQSALQRWGTMKTTKPDTVFLARPKSSPAD
                     NLLDQAVIAELRNMPPTNGVSMLQELVDLFLAAAPKRIAQISQAINDGPMLAFHAHAL
                     KSMCLSLGTKRLAELAQKLEDLGRARNVNGAPALLRELETAFAQTKVQLLPLRAQ"
     gene            3416..>3678
                     /locus_tag="VIL39_05935"
     CDS             3416..>3678
                     /locus_tag="VIL39_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY5481001.1"
                     /translation="MPTKPDRLNYGDTIGIIAPASAPPDPKNIDRSVAALERLGFKPK
                     LAPNVRKRWGYLAGSDRDRASDLMKMFADRKVKAIICVRGGYGT"
BASE COUNT          764 a         1241 c         1036 g          637 t
ORIGIN      
        1 atcatggcca gcaagccctg cgccatcgtg gttgttaccg ccaacgtcac cggccaatcg
       61 gcgaaagtct ttgaagccat gggcgccggc gccctggatg cggtcaatac gccggtcatc
      121 gggcgcactg gcgggggcga gggagccagg gccttgctgg gaaaaatcga aaccattcgc
      181 aggttgatcg gcagcgccac cgcgaggaat ccctgggctc ccgcccaacg ggtaaaacgg
      241 cacctcaccc agcatcgcaa ccacctgatc gtcatcggtg cctccgccgg cgggccgacg
      301 gccctggcca aagtgttggc ccatttgccg accgactttc ccgcacctgt gatcatcgtg
      361 cagcacgtgg attcgcactt cgtgccaggc ttggcgaact ggttgagcgg ccagaccccg
      421 ctccaggtac gcctggcccg tgaaggcgac cgcccgcaag ccggcaccgc cctggtggct
      481 ggccaggaca atcacctcgt gtttgcgagt tctacgcggc tcgtctatgt gcggcacccg
      541 gctgattgct tctaccgtcc gtccattgac ctcttcttca agagcgcgga ccagttctgg
      601 ccgggagatg tggttggggt cctgctcacc ggcatgggcc gtgatggagc cgagggactg
      661 cgcgctttgc actcgagggg ccatcatacg atcgcccagg atcagctcag cagcgcggtc
      721 tatggcatgc ccggcgccgc ggcggaactt cgcgcggcca ctgagatttt atcgctcgac
      781 aaaatcggtc ccaggctcac gaatatcgtt gcgtagagca taaaactgca tggctgaaga
      841 acacaccaac cagatcatga acttggacaa ccagatcgtg aacctagatc aggagcgccc
      901 gccgcatccg ccggattacc atgtgatggt tctgctggtg gatgaccagg caatggtctg
      961 cgaggcggtc cgccgagcac tggcaaacca aacggacatt gattttcatt actgcgccga
     1021 cgctcgcgag gccctcgcga ttgcgaacca gattaagccc acggtgattc tgcaagacct
     1081 ggtgatgccc ggcgtggacg gcctctcgct ggtcaagcaa ttccgcgcca acccggcgac
     1141 ccgggacact cccatcatcg ttctctcgac gaacgagaat ccgcaggtca aaggccaggc
     1201 ttttgccctc ggagcgaatg attacctggt gaaactgccc gataagatcg agttgatagc
     1261 ccggattcgc catcactcga agtcctacct aaacctgttg cagcgggatg ccgcctaccg
     1321 ggcgctccgc gaaagccagc agcagcttat cgaaagcaac gctgccctca tttcgctcaa
     1381 ccagaagctc gaggaagcca cgctcgccaa gtcccagttc ctggcaaaca tgagccacga
     1441 gattcgcacg cccatgaatg gcatcatcgg catgaccgcg ctgctgctgg acaccgcgct
     1501 cacggacgag cagcgggatt atgtcgaggc cacccgtagc agcggcgacg cgctactaac
     1561 gatcatcaac gacattctcg acttctccaa gatcgaatcg ggcaaggttg agttggagca
     1621 tcatcccttc gagcttcaca cctgcatcga ggaggcgatg gatttgctcg cgcccaaggc
     1681 ggcggagaag aacctcgacc tggcctgcct ggtggatgac gccattccga agattctggt
     1741 cggcgacgtg acccggctgc ggcaggttct cgttaatctc atcggcaacg ccgtgaagtt
     1801 cacccaccaa ggcgaagtgg tcgtcgaagt cacctcgtct gggcgcacgg tgcgcaacat
     1861 cccattcgga cacgagcagg acaccgagtt catccgtaac ccaaacgaat ggctgctcca
     1921 tttctcggtg cgtgacaccg gcatcggcat cccggtggat cggcagagcc gcctattcaa
     1981 gtcgtttcag caggtcgatg cctccaccac tcgccactat ggcggaaccg ggctaggcct
     2041 ggccatctgc aaacgcctgg tgaaattgct cggggggaag atctggatcg agagcgacgc
     2101 cggcaaggga tccacgttcc atttcacgat ttcggtccgg gctcctgcct ccaccacacg
     2161 cccagcgtgg caaaacccgc agccccaact cgcgggcaag cgcttgctgg tcatcgaaga
     2221 caatgccacc aaccggcgca tcatcacgca acgcggccag cagtggggca tgaccgtgga
     2281 aacggctgtc aatcgccgcg aggcgctccc gctgctggcg caaggcgatc ggtttgattc
     2341 cgtgatcctc gatttagagc tgccagacaa ggacgggctc gctctggccg aggaaatccg
     2401 caaccaacct tccggacgtt acgtgcccct gctcctgctt tcatccaagc gtccgagcgg
     2461 cgatgacgcg cacccgctgc ccgccggtgt ttctgtctct atccataaac caatccgccc
     2521 ggcgcagttg ctcgacgccc tgtgccgcgc cctgagtgtc cgggttcagc gcgagaagaa
     2581 gacgccggtc acaccgacac tcgacccaga tcttgcccgc cgcctgcccc tgcatctgct
     2641 cctggcggat gacaatccca tcaaccagaa agtcggtctc accatcctgc gcaagctggg
     2701 ctaccgcgcc gacatcgcca acaacggcct ggaagtgctc aaggcgctcg aacagaaggc
     2761 ctacgacatc ctgttcctcg acgtgcaaat gccggagatg gacggcctgg aggcggcgcg
     2821 ccaaatttgc caacgctggc cggccgagaa acgtccgcgc atcatcgcca tgaccggcaa
     2881 cgcgctaatg ggcgatcgcg agaaatgtct ccaggccggc atggacgatt acatttccaa
     2941 gcccgtccgt attgctgacc tgcagtccgc gctgcaacgc tggggcacca tgaaaaccac
     3001 aaagcccgat actgtcttcc tggcgcgccc aaaatccagt ccggccgaca acctgctcga
     3061 ccaggccgtc attgccgagt tgcgcaacat gccacccacc aacggcgtca gcatgctgca
     3121 agaactcgtg gacctcttcc tcgcagcggc ccctaagcgc atcgcgcaga tcagccaggc
     3181 gattaacgac gggccgatgc tggctttcca cgcgcacgcg ctcaagagca tgtgcctcag
     3241 cctcggcacc aaacgcctgg ccgaactggc acagaagctc gaagatcttg gccgcgccag
     3301 aaatgtgaac ggcgcccccg ccctgctccg ggaactcgaa accgccttcg ctcagaccaa
     3361 agtccaactc ctcccgctcc gcgcccaata acccgcccac tcaaggtatt cactcgtgcc
     3421 aaccaaacct gaccgcctga actacggcga caccatcgga atcatcgcgc ccgcaagcgc
     3481 gccgccggat ccaaagaaca tcgaccgctc cgtcgccgcc ctcgaacgcc tcggcttcaa
     3541 acccaagctt gctcccaatg tccgcaaacg ctggggatac ctggctggca gcgaccgcga
     3601 ccgcgcgagc gacctcatga agatgttcgc cgaccgcaag gtgaaggcga ttatctgtgt
     3661 gcgcggcggc tacggcac
//
LOCUS       DATCGS010000292         6469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_422039, whole genome shotgun sequence.
ACCESSION   DATCGS010000292 DATCGS010000000
VERSION     DATCGS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422039"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2829)
                     /locus_tag="VIL39_05940"
     CDS             complement(67..2829)
                     /locus_tag="VIL39_05940"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5481002.1"
                     /translation="MSEIPKAYEPQAVEEKWYEFWLKQNCFVANPASPKPAYSIVIPP
                     PNVTGMLHMGHVLNNTIQDILARKARMDGKEVLWLPGTDHAGIATQAVVEKTLRKQGV
                     MKHRDDLGREKFLEHVWAWKEKHGGIIIQQLKKLGCSCDWTRERFTMDPEYSRCVQKV
                     FVELYKKGLIYRGKRMVNWDPAARTALSDEEVEMVEVKGHLWHLKYPLLDDKGQPKAD
                     EFLVVATTRPETMLGDEAVAVNPTDPRYTALVGRRCLLPLQNKSIPVIADELVDPKFG
                     TGCVKVTPAHDPADYAMATRHNLPLTVVIGPDGRMTGEAGGGFAGLDRAEARKAVVEH
                     LEKLGLILKIEDYVHNVGHSQRSGVAIEPYLSEQWFLKYPSVEPSTKAVEEGRMTFHP
                     ERWSKTYAHWMGNLRDWCISRQLWWGHRVPVWYRKSKVQSPKSEVSAADIYCAVEPPA
                     DLENWEQDPDVLDTWFSSWLWPFATMGWPEQTPTLKKFYPTTDLVTGPDIIFFWVARM
                     IMAGYEFMGDLPFRNVYFTGIIRDKLGRKMSKSLGNSPDPLELIAKYGADALRFGTMR
                     SAPLGQDVLFDEKDVELGRNFCNKLWNACRFRQMQGGEVQAEIDPKLLTSDDKWILLK
                     LDQAIRDITTAFEGYRFNEATQTLYRFFWGEYCDWYVEASKAVLSSGASRGNEDPSAV
                     TNHESRITDHESRRANTVAVIDFILSHTLRLFHPFLPFITEELWHGMGYHEDMPQDQG
                     GTTIMFAHWPKPLDEDFRGHYGLDACYLDMVNTKYELVTQGRNLRREGNIPASKKVKY
                     VFKPANPLLPHDAEVLKLLLNAEALEIDAQHEPKKGTPAVHSELGELFLPLEGLIDVA
                     AEKARHTKELEKIESEITRAEQKLNNPNFTSKAPPHVLQEHQQRLAEWQAKRDRIKKA
                     LDGLQG"
     gene            complement(2836..4395)
                     /gene="cimA"
                     /locus_tag="VIL39_05945"
     CDS             complement(2836..4395)
                     /gene="cimA"
                     /locus_tag="VIL39_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY5481003.1"
                     /translation="MKPEVEIYDTTLRDGSQGEGINFSVKDKLRIAEKLDAFGVHYIE
                     GGWPGSNPKDIEFFAEARRRKFKHARLAAFGSTRRKGVAVESDDQVRLLIEAQTPVIT
                     IFGKTWLLHVKEVLRTTPEENLAMVGDTVRFLKDHGKFVIYDAEHAFDGYKDDPEYAL
                     ATWQAAEKAGADFVVLCDTNGGCLPREVAAITAAARGKLNVSLGIHTHDDIGLGVANA
                     LAALEAGATHVQGTINGYGERTGNCNLTSVIPNVAFKMKKRCVPEKSLTQLRELSQFV
                     DDIANFRHNPRQPWVGQTAFAHKGGMHVNAVQKVARSFEHIDPALVGNRRRVLVSDLA
                     GKSNIVMKAQELGFRVTNDTPELKTILARIKELEHEGCEFEAAEASLALLISRELQHK
                     KPPFSVDGYHVSMRRDGPNSVCEATVKVRVGAEAGHTVADGDGPVNALDKALRGALVR
                     FYPRLKKVVLTDYKVRILESSTGTGARTRVLIQSTDGKEEWGTVGMSDNIIEASLQAL
                     VDSLEYALTRK"
     gene            5055..>6469
                     /locus_tag="VIL39_05950"
     CDS             5055..>6469
                     /locus_tag="VIL39_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481004.1"
                     /translation="MNSWTDQQLIRDYTGSRSEPAFAELARRHVDFVYSAALRMVRDT
                     HLAEDVTQAVFVALAQSAAQLTDRPALSGWLHRTTQNLAANAVRSDVRRRAREQEAAV
                     MHEILSAEPDAGWEQIAPQLDAALGELSEGDREALLLRYFERKSAGEMAQTLGISDEA
                     AQKRVNRAVERLRELFAKRGVAVGASGLVAVISANAVQAAPASLVALVSTSAALGGTT
                     LVTTATATKAIAMTALQKTLVTALITAAVGTGIYEARQASTLRSQVQTLQQQQVPLAD
                     QLAALQTENERLSNQVAQPKDSQTLPPAQLNELLKLRSKATLAQADSHELARLKATVA
                     RQTGQVPDYFTNAMATGLATANKWKQKDALARLARMKTMLNLSADQEQAISNIMTNHI
                     QRQSQMTLSLMTGKLTPEEAKAQAGASGDEQAEIKALLTPEQLAAYPGDVQAEKTTAA
                     NNSAASEASQITANFSLPKDQ"
BASE COUNT         1193 a         2053 c         1928 g         1295 t
ORIGIN      
        1 tgtcccaaga gtgcctgccg gttgagaaag aatcaacctt ccactgagtg cccgcagcgc
       61 ggtcgctcag ccctgcaaac cgtccagcgc tttcttgatc cggtcgcgtt tagcctgcca
      121 ctccgcaagg cgttgctgat gctcttgcag cacatggggt ggcgctttgg aggtgaagtt
      181 gggattgttc agcttctgct cggctctggt gatttcgctt tcgatcttct ccagctcttt
      241 tgtatggcgc gccttctccg ccgccacatc aatcaatcct tcgagcggca gaaacaattc
      301 gcctagctcg gagtggacgg caggggtccc tttcttcggc tcatgctggg cgtcgatttc
      361 cagcgcttcg gcgttaagca gcagctttag gacttcggca tcgtgtggca aaaggggatt
      421 ggccggctta aagacatact taaccttctt gctcgctggg atgttgcctt cgcggcgcag
      481 gttgcggccc tgggtgacca actcatactt ggtattgacc atgtcgaggt agcacgcgtc
      541 caggccatag tggccgcgga aatcctcatc cagcggcttg ggccaatgcg caaacatgat
      601 ggtggtgccg ccctggtctt gcggcatatc ttcgtggtaa cccatcccgt gccagagttc
      661 ctcggtaatg aacggcagga acggatggaa taggcgcagc gtgtgcgaga ggatgaagtc
      721 aatgaccgcc acggtgttgg cgcggcggga ttcgtgatcc gtgatgcgtg attcgtgatt
      781 cgtgacggcg gatggatctt cgttgcctcg gctggcaccg gaagagagca cggctttgct
      841 ggcctcgaca taccagtcgc aatactcgcc ccagaagaag cggtagagcg tttgcgtggc
      901 ttcgttgaac cgatagccct cgaacgccgt ggtgatgtca cgaatggcct ggtccagctt
      961 gagcaatatc catttgtcgt cactggtcag gagcttggga tcaatctcgg cctgcacctc
     1021 gcccccttgc atctggcgga agcggcaggc attccacagc ttgttgcaga agttgcggcc
     1081 cagctcgaca tccttttcgt cgaacagcac atcctggccg agcggcgcgc tgcgcatggt
     1141 gccgaagcgc agggcgtcgg cgccatactt ggcgatgagt tccagcgggt cgggggagtt
     1201 gcccaggctc ttactcatct tgcggcccag cttatcgcgg atgatgccgg tgaaatagac
     1261 gttgcggaag ggcaggtcgc ccatgaactc gtagcccgcc atgatcatcc gcgccaccca
     1321 gaagaagatg atgtccggtc cggtgaccag atcggtggtg gggtagaact tcttgagcgt
     1381 aggcgtttgc tccggccagc ccatcgtggc aaagggccac agccaggagc tgaaccaggt
     1441 gtccaggacg tcagggtcct gctcccaatt ctccagatca gccgggggct ccaccgcgca
     1501 atagatgtcg gcagcggaga cttcagactt tggactttgg actttggact tccgatacca
     1561 gaccggcacg cggtgccccc accagagctg gcggctgatg caccagtccc gcaaattgcc
     1621 catccaatgc gcgtaggtct tgctccagcg ttcggggtgg aacgtcatgc gcccctcctc
     1681 gaccgccttg gttgacggct ccacgctcgg atacttcaga aaccactgct cgctcagata
     1741 cggttcgatg gcgacgccgc tgcgctggct gtggccgacg ttgtggacgt agtcctcgat
     1801 cttgaggatc aggcccagct tctccaaatg ctccacgacg gccttgcgcg cttccgcgcg
     1861 atcgaggccg gcaaagcctc cgccggcttc cccggtcatt cgcccgtccg ggccgatgac
     1921 gacggtcaat ggcaggttgt gccgcgtggc catggcgtag tcggctgggt cgtgcgcagg
     1981 ggtcaccttc acgcagccgg tcccgaactt cgggtccacc agctcatcgg ctatgacagg
     2041 gatggatttg ttctgcagcg ggagcaggca gcggcgtccg accagcgcgg tatagcgcgg
     2101 atcggttggg ttcaccgcca cggcctcatc accgagcatt gtttccgggc gggtggtggc
     2161 gaccacgagg aattcgtctg ccttgggctg tcccttatca tcgaggagcg gatactttag
     2221 gtgccacaga tggcccttta cctccaccat ctcgacctcc tcgtcggaga gcgccgtgcg
     2281 cgctgccgga tcccaattca ccatgcgttt gccgcggtag atgaggccct tcttgtatag
     2341 ctcgacgaag accttctgca cgcagcgcga gtattccggg tccatcgtga accgttcgcg
     2401 cgtccaatcg cacgagcacc ccagcttctt aagctgctgg atgatgatgc cgccgtgctt
     2461 ttctttccag gcccaaacgt gttcgaggaa cttctcccgg cccaggtcgt cgcggtgctt
     2521 catgacgccc tgcttccgga gcgtcttttc caccacggcc tgcgtcgcaa taccggcgtg
     2581 atcggtgccg ggcagccaga gcacttcctt gccgtccatg cgcgccttgc gggcgaggat
     2641 gtcctggatg gtgttgttca ggacgtgccc catgtggagc atacccgtga cgttgggggg
     2701 cgggatgaca attgaatagg ccggtttggg cgaagcgggg ttggccacga agcaattctg
     2761 cttcagccag aactcatacc acttctcctc aaccgcctgg ggttcgtaag ccttgggtat
     2821 ttcagacatg gtaactcact tccgcgtcag cgcatattcc agactgtcca ccagcgcctg
     2881 gaggctggct tcgatgatgt tgtcgctcat ccccacggtg ccccattcct ctttgccgtc
     2941 ggtggactgt atgagcaccc gcgttcgagc gcccgtgccc gtggaggatt ccaggatgcg
     3001 caccttgtaa tcggtcagca ccaccttctt gaggcgcgga tagaacctga ctaaggctcc
     3061 gcgtagcgcc ttgtcgagag cgttcacggg tccgtcgccg tcagccactg tatggccggc
     3121 ttcagcgcct acgcgcactt tcacagtcgc ctcacagacc gagtttggcc cgtcgcgccg
     3181 catcgagaca tggtagccat ccactgagaa gggcggtttc ttgtgctgca gctcacggct
     3241 gatcagcagc gccagcgagg cctccgctgc ctcgaattcg cagccctcgt gctccagttc
     3301 cttgatgcgc gccaggatgg tctttagctc cggcgtatcg ttcgtgaccc ggaagcccaa
     3361 ctcctgcgcc ttcatgacga tgttgctctt gccggccagg tcgctgacca gcacgcgccg
     3421 ccggttgccc actaacgccg ggtcaatgtg ctcgaagctc cgcgccacct tctgcaccgc
     3481 gttgacgtgc atcccgccct tgtgggcaaa tgccgtctgg cccacccacg gttgccgcgg
     3541 gttgtgccgg aagttcgcga tatcatccac gaactgcgaa agctcccgca actgcgtcag
     3601 cgacttctcc ggcacgcacc gcttcttcat tttgaaggca acgttgggaa tcacgctggt
     3661 gaggttgcag ttcccagtgc gttcaccgta accgttgatc gtgccctgga catgggtcgc
     3721 gcccgcttcg agggctgcca gcgcgtttgc cacccccagg ccgatgtcgt cgtgggtatg
     3781 gatgccgagg ctcacgttta gcttcccgcg ggccgcggca gtgattgcag ctacctcacg
     3841 cggcaggcag ccgccgttgg tgtcgcacag gaccacgaaa tccgcccctg ccttctcggc
     3901 cgcctgccag gtcgccaggg cgtattcggg gtcgtccttg tagccatcga aggcgtgctc
     3961 ggcgtcgtaa atgacgaatt tgccgtggtc tttcaggaag cgcaccgtgt caccgaccat
     4021 ggccaggttc tcttcgggcg tcgtcctcag gacttccttg acatgcagca gccaggtctt
     4081 gccgaagatc gtgatcacgg gcgtctgcgc ctcgatcagc agccggacct ggtcgtcgct
     4141 ttccaccgcg acacccttgc gccgcgtcga accgaaggcc gccagtcggg catgcttgaa
     4201 cttcctgcgc cgcgcctcgg caaagaactc gatgtccttg ggattagacc ccggccagcc
     4261 gccctcaatg taatgaaccc cgaacgcatc gagcttctcg gcgatgcgca gcttgtcctt
     4321 cacggaaaag ttgatgcctt cgccctggct gccgtcgcgc agggttgtat cgtatatttc
     4381 gacttcgggt ttcatagcaa tgcccgccaa agcgggtgtg aatatctacg ctaagccaga
     4441 ctggatgcaa agtccaaacg cgcggactgc tggcgggttg gcgtatcctc gcaccgccac
     4501 cgttcttagc cttggtgact tggccggagc gcggcggtcc ccagccgcag cacgtccgca
     4561 aggagttggg ggtcgaaaga ctcgagcagc gttgcgtggc cggtgctgct gcggctgggg
     4621 acagccgcgc tccggtcgca gcctcaaagt gcgcccccgg cgcattgatg ggagaacacc
     4681 atgctgtttg aatagcggtc aaaccaacgc tgtcaaaact acggttcggc tgggtgtggc
     4741 cgcgaggctt acaggggaaa aacaatagga gaataccaag agaatacgaa ggaaacacca
     4801 agagaatacg aagagaatac gaagagaata cgaagagaat accatagcaa cacgcgcgca
     4861 tcccccatgc tcatggcttg ccggcggctg gtaactggct ttggggtggc ttgacccgcg
     4921 cgctcctctg cgtatctcct cggaagtggc agaccgagca ttcaggcatt cccggcgagg
     4981 gggcttcttc atgaataatc ccccgccgct cctttccctg tccgatttgg acggcggcgg
     5041 ttactatact gtgagtgaac agttggaccg accagcaact tatccgcgac tacaccggga
     5101 gccgctccga gccggcattt gccgaactcg cccgccggca cgtggacttc gtctattccg
     5161 ccgcgttacg gatggttcgc gacacgcacc tggctgagga cgtgacgcag gccgtatttg
     5221 tagcattggc gcaaagtgcg gcgcaactca cggaccgtcc cgccctgtcc ggctggttgc
     5281 atcgcaccac gcaaaacctc gcggccaacg ccgtccgctc cgatgtgcgc cgtcgtgccc
     5341 gtgaacagga ggccgccgtc atgcatgaaa tactatccgc cgaacccgac gccggctggg
     5401 aacagatcgc cccgcagctc gacgccgcgc tcggggagtt gagcgagggg gatcgggagg
     5461 cgctgctgct ccgttacttc gaacgcaagt ccgccggcga gatggcgcag accctcggca
     5521 tcagcgacga ggccgcgcag aagcgcgtca accgcgccgt cgagcgtctg cgcgaactct
     5581 ttgccaaacg cggcgtcgcc gtcggcgcga gcggcctggt tgccgtcatc tccgccaacg
     5641 ccgtgcaggc cgcgccggcc agcctggtcg ccctggtttc aacctccgcc gctcttggcg
     5701 gcacaaccct cgtcaccact gcaaccgcaa ccaaagccat cgctatgacc gctctccaaa
     5761 agactttagt caccgccttg atcaccgccg ccgtcggcac gggaatctat gaagcccgcc
     5821 aggcatccac cctgcgcagc caggttcaaa cgctccaaca acagcaagtg ccgttggctg
     5881 atcagttggc ggcgctccag actgagaacg agcgtctgtc caatcaggtg gcccagccca
     5941 aagattccca gactctgccg ccagcgcaat tgaacgaatt gttgaagctg cgcagcaaag
     6001 cgaccctcgc gcaggcggac tcccacgaac ttgcccggct caaagccacg gtcgctcgac
     6061 agacggggca ggtaccggat tacttcacca acgcgatggc gacaggcttg gccaccgcca
     6121 acaagtggaa gcagaaggac gcgctcgccc ggctggctcg catgaaaacg atgcttaacc
     6181 tctcggccga ccaggagcag gccatcagca acatcatgac gaaccatatc cagcgccaat
     6241 ctcaaatgac gctgagcttg atgacaggca agctgacgcc cgaggaagca aaagcccagg
     6301 ccggcgcttc cggcgacgag caagctgaaa tcaaagccct gctcactccc gaacaattgg
     6361 cagcctatcc gggcgatgtg caggcggaga aaacgactgc cgccaacaac tccgccgcgt
     6421 ccgaggccag ccagatcacg gccaatttca gtcttcccaa agaccagca
//
LOCUS       DATCGS010000293         5645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_24466, whole genome shotgun sequence.
ACCESSION   DATCGS010000293 DATCGS010000000
VERSION     DATCGS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24466"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VIL39_05955"
     CDS             complement(<1..339)
                     /locus_tag="VIL39_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5481005.1"
                     /translation="MRLWLGAEEMNAELALTGEQMQTGMMFRTNLAENAGMLFVFPMP
                     HRASFWMKNCPLPLSAAYINPEGVILEIHELKPHDTNSVVASSEQVQFVLEANQGWFG
                     RHNVRAGMLVR"
     gene            complement(635..1879)
                     /locus_tag="VIL39_05960"
     CDS             complement(635..1879)
                     /locus_tag="VIL39_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY5481006.1"
                     /translation="MNAVTPPQIASPSPSRLMSLDVFRGATIAAMILVNNPGNWGAIY
                     KPLDHAEWHGWTFTDLIFPFFIWIVGVAIPLSTARRLEQGQSRRQLLLHALRRSVIIF
                     ALGFFLNSFSYFIDGSLFRDGFSAWFHNYATNVRVPGVLQRIGICYFIATLIFLGTGI
                     RGQIIWIAGLLAGYWGLMIAGPWFGWGTGGLEQHGNFSEYLDNLVLNGKVIGMHVWKG
                     GKTWDPEGIFSTVPAIATCLFGIMTGHLLGSKQTPETKTAWLFVGGNLLLFAGVLMDV
                     WLPINKKLWTSSYSVFMAGMAMNCFAVSYWLVDVQGWRKWAKPFAIYGMNAIAVFMLS
                     GLLGRILPAIKVGTAAGKKVGLQSYLYDNLFAPFASPDTAPFFSFLASPKNASLMWAL
                     MYITGLYLAAYGMYRRKWFLKF"
     gene            2046..2939
                     /locus_tag="VIL39_05965"
     CDS             2046..2939
                     /locus_tag="VIL39_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5481007.1"
                     /translation="MNLNRRNFLKATGLGIGGLSLRPTSSLGAEAADKAGVPARRPTM
                     HLGLVTYNLAADWDVPTIIKNCEAAKFEGVELRTSHAHKVEVNLSKDERKEVKKRFAD
                     SKVQLMGLCAIFDYHTPDQAKLRKDIEATKEYIILAEDVGATGVKVRPNAFPKEVPQE
                     KTLEQIGHALREVGDFARDHGQVIRLEVHGTGTSFPPNIKKILDVADHPSVGACWNSN
                     ASDLAGQGWDYNFDLLKDKIFSVHITELFQAEYPYRKLLTRLNEINFKGFCLAEISAS
                     TDPIRVMKYYRALWLAYQGLL"
     gene            complement(2946..4085)
                     /locus_tag="VIL39_05970"
     CDS             complement(2946..4085)
                     /locus_tag="VIL39_05970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY5481008.1"
                     /translation="MNRRQFLRTTGIVSAVTLLGLPTKAADSENEGVLDINPTHDNPR
                     NSEGSFVTLKSGRILFIYTRFQGGAADESPAQLVSIHSDDGGRTWSREPRTVVENPPG
                     ANVMSVSLLRLRSGPIVLFYLAKNSLLDCRPVMRISTDEAETWSEPRHVGDAPGYFVL
                     NNDRVIQLQGGRLVLPLAFHRSRGSDPRNYRSLDMRAIALWYWSEDEGKTWQEADDWW
                     ALPARTTTGLQEPGVVELADGRLFSWMRTDQGTQFGCYSTDSGRRWSSPEATDLQSPA
                     SPASIKRLPGSSDLLALYNDHSGRFPFPKGKRSPLVAAISSDGGKTWPRRKLLEGDPD
                     GWYCYTAIHFTEDAVLLAYCAGDPKVGHLNRLRLRRISLNRLKET"
     gene            complement(4124..4684)
                     /locus_tag="VIL39_05975"
     CDS             complement(4124..4684)
                     /locus_tag="VIL39_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5481009.1"
                     /translation="MKRAVFLDRDGTIIEEKNYLRRVEEVVIFPRAAVALRRLQSAGF
                     SLFIVSNQSGVGRGYYTLADVEKVNQHLQEELGREGVRFEKIYIAPEAPGAPSRGRKP
                     SPQFLLDARDAFGVDLAQSYMIGDKLIDLECGWNAGVKKCLLVRTGYGAELERASADK
                     LGAAAVVDDLSTAAQWILEQTGRSPV"
     gene            4837..>5645
                     /locus_tag="VIL39_05980"
     CDS             4837..>5645
                     /locus_tag="VIL39_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5481010.1"
                     /translation="MSESYIQSLFAERIGGSQYGKSQAIYKFEKIKRAKRAAMTAHPG
                     AEIIDLGIGEPDEMAFPEVVEKLCAEARRPENRGYADNGDAVLKQAAARYLDRVCGVK
                     SINPDTEVLHSIGSKAALSILPGALINPGDYVLMTVPGYPVFGTHAKYLGGRVHNLPL
                     TEENQFLPNLASVPAEVLKQAKVLVLNYPNNPTGASATPEFFAQAVEFARKNRLIIIH
                     DAAYAALVFEGQPLSFLATPGAKEVGVELHSASKSFNMTGWRCGFVAGNELL"
BASE COUNT         1209 a         1693 c         1682 g         1061 t
ORIGIN      
        1 gcgaaccagc atccccgccc tgacattgtg tcgcccgaac cagccttggt tggcttcgag
       61 cacaaactgc acctgctccg aggatgccac gacggaattc gtgtcatggg gcttcagctc
      121 gtgaatctcc aggatgacgc cttctgggtt gatgtaagct gccgacaatg gcaagggaca
      181 gttcttcatc cagaacgacg cgcggtgcgg catcgggaag acgaaaagca tccctgcgtt
      241 ctccgccagg ttcgtgcgaa acatcatgcc ggtttgcatt tgctcgccgg ttaacgccag
      301 ctcggcgttc atttcctcag cccccagcca taatctcata gttggcagtt tcggttgcgc
      361 caaggcgggc agactcggat ccgcctccgg tccggtcgcc ggcgacgccg gtttgttgca
      421 gccaccagca cagccaccgc acacaacgac cgccagcagc gtcaggatgc gcaatcggtt
      481 catagcccaa gcatgagcga acgccgctgg gatttcaact ccgatcggaa gagcggggcg
      541 ggtggtaagc tgttgctact ttatcgcagg gcttgaaatt ctcccacccc tgctgcaacc
      601 tgtgtaactg atttaacccc tgtaactctt ccgctcagaa tttaaggaac cacttgcgcc
      661 ggtacatgcc gtaggcggcg aggtagagcc cggttatgta cattagcgcc cacatcagtg
      721 aggcgttctt cgggctggcc aggaagctga agaacggcgc ggtgtcgggg ctggcaaagg
      781 gagcgaagag gttatcgtag aggtagctct ggagcccaac cttcttgccc gcagcggtcc
      841 ccaccttgat agccggcaaa atccgcccca acaagcccga cagcatgaat acggcgatcg
      901 cattcatgcc gtagatggcg aacggtttgg cccacttgcg ccagccctgc acatccacga
      961 gccagtaact cacggcgaag caattcatcg ccatgccggc catgaacacg gagtaggagc
     1021 tggtccagag cttcttgttg atcggcagcc agacatccat cagcactccc gcgaagagca
     1081 gcaggttgcc ccctacgaac agccaggcgg tcttggtttc gggtgtctgc ttggacccga
     1141 gcaaatggcc agtcatgatt ccaaacaggc aggtggcaat cgccgggacg gtgctgaaga
     1201 tgccctcggg gtcccaggtc tttccaccct tccaaacgtg catgccgatg accttcccgt
     1261 tcagcaccag gttgtcgagg tactccgaga aattgccatg ctgctccagg ccgccggtgc
     1321 cccagccgaa ccagggcccc gcgatcatca gcccccaata gccggccagc agccccgcaa
     1381 tccaaatgat ttgaccgcga atgccggtgc ccaaaaaaat cagcgtcgca atgaaatagc
     1441 aaatcccgat gcgctgcagc acgcccggga ctctgacatt cgtcgcgtag ttatggaacc
     1501 aggctgaaaa gccatcgcgg aagagcgagc cgtctatgaa atacgagaag gagttcagga
     1561 aaaagcccag cgcaaagata atcaccgagc ggcgcaacgc atgaagcaac agttgccggc
     1621 ggctctgacc ctgctccagc cggcgcgcgg tcgaaagcgg aatggccacc ccgacaatcc
     1681 agatgaagaa tgggaagatc aaatcggtga acgtccaccc gtgccattcg gcatggtcca
     1741 gcggcttgta aatggcgccc cagttgccgg gattgttcac caggatcatc gcggcaatcg
     1801 ttgccccccg gaagacatcg agggacatca gtcgggatgg gcttggcgag gcaatctggg
     1861 gcggcgtgac ggcgttcatg atctgtcggc gccaaggcta cgaatattcc ctctgcaaac
     1921 aaagggcgaa agcgaggcgg gcgggaaaga gcagcgcgta tgcctgaagc cggaaaagac
     1981 tggaatcgaa gagcggaaat caaatagcct tgaatgtctc ccggttcctg ttacaacccg
     2041 gcgtcatgaa tctcaatcga cggaactttc tcaaagcgac gggactggga attggcggcc
     2101 tgagcctccg gccaacgtca tccctgggtg ccgaagcggc tgataaagcc ggcgttccag
     2161 cccgccgtcc aaccatgcac ttggggctgg taacctacaa cctggccgcc gactgggacg
     2221 ttccgaccat tatcaagaac tgtgaggcgg caaagttcga aggagtggaa ctgcgcacca
     2281 gccacgcaca caaggtcgag gtcaatctat ccaaggacga gcggaaggaa gttaagaagc
     2341 gattcgccga ctcgaaggtg cagttgatgg gactgtgcgc tatctttgat taccatacac
     2401 ccgatcaagc gaagttgcgc aaggatatcg aagcgactaa ggaatacatc attctggcgg
     2461 aggatgtggg cgcgaccggt gtgaaggtgc ggccaaatgc ctttcccaag gaggtgcccc
     2521 aggaaaagac gctggagcag atcggacacg cgctacgaga ggtcggcgac ttcgcccgcg
     2581 accacgggca ggtcatccgg ctggaggttc atggcaccgg cacctcattt ccgcccaaca
     2641 tcaagaagat cctcgacgtg gctgaccatc caagcgtggg cgcctgctgg aactcgaatg
     2701 cgtccgattt ggcaggccag ggctgggatt acaacttcga cttgctcaag gataagatat
     2761 tctccgtgca catcacggag ttgtttcagg cggagtatcc gtatcgcaag ctgctgacgc
     2821 gcctgaacga gatcaacttc aagggtttct gcctggctga gatttcggcc agcactgacc
     2881 ccatccgcgt aatgaaatac taccgcgccc tttggctcgc ttaccagggg ctgctctagc
     2941 gttgctcagg tctccttgag ccggttgaga cttatgcgtc gcagccggag ccgattcagg
     3001 tgcccaactt tggggtcgcc ggcacagtag gctaataaca cggcatcctc ggtgaaatgg
     3061 atcgccgtgt agcaatacca gccgtccggg tcaccctcga gcagcttgcg ccgcggccag
     3121 gtctttccgc catcgctaga gatggcggca accagcggcg agcgtttgcc tttggggaag
     3181 ggaaaccgac cggaatgatc gttgtagagg gcgagcaggt ccgatgagcc gggcaagcgc
     3241 ttgatgctgg ccggcgacgc cggggactgc agatcagttg cttcgggtga cgaccagcgc
     3301 cttccactat ctgtgctgta acagccgaac tgggtgccct ggtcggtgcg catccaactg
     3361 aacaggcggc cgtcggcgag ttcgacgacg cccggttcct gcagaccggt ggtggttcgc
     3421 gccggcaagg cccaccagtc gtcggcttcc tgccaggtct tgccttcgtc ctccgaccaa
     3481 taccaaagcg caatggcccg catatccagc gagcggtagt ttcgcggatc agagccgcgc
     3541 gagcgatgga aggccaacgg caataccaga cggccgccct gaagctgaat gacgcggtcg
     3601 ttgttcaaca cgaagtagcc gggggcatcg cccacgtgcc ggggttcgga ccacgtctcg
     3661 gcctcgtcag tggaaattcg cataaccggc cggcaatcca acaggctgtt cttggcgaga
     3721 tagaacaaga cgattggccc gctgcgcaac cgcaacagcg agacgctcat cacgttggct
     3781 ccaggcgggt tctcgaccac cgtcctgggt tcccggctcc aggtccgccc gccgtcatcg
     3841 gaatgaatgc ttacgagttg agcgggactt tcatcagccg cgccgccctg gaagcgggtg
     3901 tagatgaaca gaattcgccc tgacttcagc gtgacaaagg agccttcgga attgcggggg
     3961 ttgtcgtgag tgggattgat atccagcacc ccctcgtttt ccgaatcagc cgctttggtc
     4021 ggaaggccga gcagggtcac cgccgatacg atgccggtgg tccgtaagaa ctgtcttcga
     4081 ttcatagata ggtcgtcctc tggtcaagac agcgattatc ctgctacacg ggactgcgtc
     4141 cagtttgttc gagtatccac tgcgccgccg tggacagatc gtccaccacg gcagcggcgc
     4201 ccagtttatc agccgacgcg cgctccaatt ccgccccgta accggtgcgg acgagtaaac
     4261 acttcttgac gccggcattc cagccgcatt ccagatcaat cagcttgtcg ccaatcatgt
     4321 agctttgcgc caggtccacg ccgaatgcat cgcgcgcatc caggaggaat tgcggcgagg
     4381 gcttgcggcc gcggctgggc gcgccggggg cctcgggcgc gatgtaaatc ttctcgaagc
     4441 gcaccccttc acggccaagt tcctcttgca ggtgctggtt aactttctcc acgtccgcca
     4501 gtgtataata cccccggcct acacccgact ggttggacac aatgaataag ctgaacccgg
     4561 cgctttgcag tcgccgcagc gccacggccg ccctcgggaa gatgaccacc tcttccacgc
     4621 gtcggaggta gttcttctcc tcaataatcg tcccatcgcg gtctagaaaa acggctcgct
     4681 tcatcttcaa gtcaggcgga attgttaagg cagcgctcca gcaatggaaa tgataaaagc
     4741 gccgcccctt gcattggtct cggcatttgc ttagtattgc cagccgagac gaggaccagt
     4801 ttctgccgct ggcaagacga cggcccttgt aagcttatgt ctgaatcata catccagagc
     4861 cttttcgcgg agcgcatcgg cggcagccaa tacggcaagt cgcaggcgat ttacaagttc
     4921 gagaagatta aacgcgccaa gcgcgccgcc atgaccgcgc acccgggcgc ggaaatcata
     4981 gacctcggca tcggtgagcc ggatgagatg gcattcccgg aggtcgtcga gaagctgtgc
     5041 gcggaggcgc gcagacccga gaaccgcggc tacgcggaca acggtgacgc ggtcctgaaa
     5101 caggcggcgg cccgttatct ggaccgggta tgtggcgtca agagcatcaa tccggataca
     5161 gaagtccttc actcgatcgg cagcaaggcg gcgctctcga tcctgcccgg ggcgctcatc
     5221 aacccgggcg attatgtgct gatgaccgtg ccgggttacc cggttttcgg cacgcatgcc
     5281 aagtatctcg gcgggcgggt ccacaatttg ccgctgacgg aggagaacca gtttctgccg
     5341 aacctggctt ccgtgcctgc ggaggtgctc aagcaggcca aggtgctggt gctgaactat
     5401 ccgaacaatc cgaccggcgc cagcgcgacg ccggaattct ttgcccaggc agtcgagttc
     5461 gcgaggaaga accggctcat catcatccat gacgcggcct acgcggcgct ggtgttcgaa
     5521 ggtcagccgt tgagctttct cgcgacgccc ggcgcgaagg aagtcggcgt tgaactgcac
     5581 tccgccagca agtcgttcaa catgaccggc tggcgctgcg gctttgtggc ggggaacgaa
     5641 ctgct
//
LOCUS       DATCGS010000294         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_472287, whole genome shotgun sequence.
ACCESSION   DATCGS010000294 DATCGS010000000
VERSION     DATCGS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472287"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1081)
                     /locus_tag="VIL39_05985"
     CDS             complement(44..1081)
                     /locus_tag="VIL39_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5481011.1"
                     /translation="MPILLKNQTSAQLHATLAHAGVSPHLARRLLAASVKRGELPAPG
                     SDLTTRLRNELLVLTEIPHLTQVQKVVSPQDGFTRYLFEGQGIGQFEAVRIPLLHRPD
                     DLKYVACVSSQVGCAMGCVFCATGRMGFGRNLAAWEIVDQVVKMQADSPHPIRGVVFM
                     GMGEPLLNYDAVMTAIRILSEPCGMAIAAKAITLSTVGIIPGIRRFTAERHRARLVVS
                     LTSSDPLQRRELLPVEQGNPTPDLIQALREYHAVSGQRVTLAWTMISGVNVGGGDAQR
                     LADLTHGLPIKLDLIDVNDPSGRFRPPSRAELDAFRDALREKLGMPVARRYSGGQDIH
                     GACGMLAGGRA"
     gene            complement(1143..1622)
                     /locus_tag="VIL39_05990"
     CDS             complement(1143..1622)
                     /locus_tag="VIL39_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY5481012.1"
                     /translation="MKKAQLIRQIIASLTESLVVLEKAARASHEEATHESSRAESKYD
                     TRGLEAAYLAGGQARQAKEIMDSIKAYEALATRDFAPGEPIDLTALVELDVDGTRATY
                     FIGPKSGGLEIKHRGKEIMVITPQSPLGQNLMGRKAGQRWAAKLGGSTVKYQIVSVS"
     gene            complement(1707..2339)
                     /locus_tag="VIL39_05995"
     CDS             complement(1707..2339)
                     /locus_tag="VIL39_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEY5481013.1"
                     /translation="MKTDMKALAAFALLTGLLCGSALADTVNFDDLKAGAPPPGWTAS
                     RTGKGDAKWEIVADDSAPSKTNVLRQSGEATYPVCFKNDASLKDGFVAVKFKAVSGKI
                     DQAGGVIWRCQDADNYYIARANALEDNITIYHTIKGKRVSLKNIDTTVAPGVWHTLRV
                     EFKGNKFAVIFDSKKVIEATDDSFSDAGKVGVWTKADSVTLFDDFSYGSK"
     gene            2416..>2560
                     /locus_tag="VIL39_06000"
     CDS             2416..>2560
                     /locus_tag="VIL39_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY5481014.1"
                     /translation="MKWITREKVKVGRVARPWRIRKFIDPEAEFIVCDALYAECERRA
                     GPAA"
BASE COUNT          465 a          767 c          791 g          537 t
ORIGIN      
        1 ccgatgagca ccaccagcgc tccgagtgcg ctcctggccg gttttacgcc ctcccgcccg
       61 ccagcatgcc gcaggcgcca tggatgtcct ggccaccact gtaacgccgg gccacgggca
      121 tgcccagctt ctcgcggagc gcatcgcgga aggcatccaa ctcggcacgc gagggcgggc
      181 gaaagcgccc ggacggatcg ttgacgtcaa tcaggtcgag cttgattggc aggccgtggg
      241 tcaagtccgc cagcctttgg gcgtcgcccc cgccaacatt caccccggat atcatggtcc
      301 atgccagcgt gaccctttgc ccgctgacag cgtgatactc tcggagcgcc tgaatgaggt
      361 ccggcgttgg attgccctgt tcgacgggca acaattcccg ccgttggagt gggtccgagg
      421 aagtcagcga gacgaccagt cgggcgcgat gccgttcggc cgtaaagcgg cggatgccgg
      481 ggatgatgcc gaccgtcgag agcgtgatcg ctttggcggc gatggccatc ccgcatggtt
      541 cggatagaat tcggatggcc gtcatcaccg cgtcgtaatt gagcagcggc tcccccatgc
      601 ccatgaagac caccccgcga atgggatgcg gcgaatccgc ctgcattttg acgacttgat
      661 ccacgatttc ccacgcggcg aggttgcggc caaaacccat ccggcccgta gcgcagaaga
      721 cgcatcccat ggcgcagccg acctgggaac tgacgcaggc cacatacttg aggtcgtccg
      781 gccggtggag gagcggaatg cggaccgcct caaactgtcc gatcccctgc ccttcaaaca
      841 gatacctggt gaatccgtcc tgcggcgaga cgactttctg gacctgcgtc aaatgaggaa
      901 tctccgtcaa gacgagcagc tcattccgta accgggttgt aaggtcgctg cccggagcgg
      961 gcagttcgcc gcgtttcacg gaagccgcca aaagccgccg cgccaggtgc gggctcactc
     1021 cagcatgggc cagagtggcg tgcaactgtg cggaggtctg gttcttcaac aggatcggca
     1081 tattcgcatt cgcctctaac gtcgaggcga gctatcatgc gttccccggc gtcggtggct
     1141 aatcacgaaa ccgagacaat ctggtatttc acggtcgaac cgccaagctt ggccgcccag
     1201 cgctggcccg ctttcctgcc cattaagttt tggcccagcg gcgactgggg cgtaatcacc
     1261 atgatctcct tgccccggtg cttgatttcc aggccaccgc tctttggccc gataaaatag
     1321 gttgcacgtg tgccatcgac atccagttcc accaaggccg tcaaatcaat cggctcaccg
     1381 ggggcgaaat ccctggtcgc tagcgcttcg taagccttga tggagtccat gatttccttc
     1441 gcctgcctgg cctgaccgcc agcgaggtag gccgcttcga gtccacgggt gtcgtatttg
     1501 ctttccgccc ggctgctctc gtgcgtggcc tcttcgtgcg atgcgcgggc ggccttttcc
     1561 agaacgacga ggctctcggt caggctggcg ataatctgtc ttatcaactg ggctttcttc
     1621 attttgcggc gatgtgtcaa gctggcatga taaaccaaag aaatccagct tgtggagcag
     1681 caaccaggca gcacgtgtaa tggcgcttac ttgctcccgt aactgaagtc gtcgaacagc
     1741 gtcacactgt cggccttggt ccaaacgccg actttgccgg cgtcggagaa gctgtcgtcg
     1801 gtggcttcga tgaccttctt gctgtcgaag atgacggcaa acttgttgcc cttgaactcg
     1861 acgcgcagcg tgtgccagac gccgggagcg actgtcgtgt cgatgttctt caaggagacg
     1921 cgtttgccct tgatggtgtg gtagatggtg atgttgtctt cgagggcgtt ggcgcgggcg
     1981 atgtagtagt tgtccgcgtc ctggcagcgc caaatgacgc cgcccgcctg gtctatcttg
     2041 ccggagacgg ccttgaactt cacagccacg aagccgtcct tgaggctggc atcgttctta
     2101 aagcagaccg gataggtggc ttcgcccgat tgcctgagga cgttggtttt gctgggggcg
     2161 gagtcgtcgg ccacgatttc ccatttggca tcccctttgc cggttctgga agcggtccaa
     2221 ccgggcggcg gcgcgccggc tttcaggtcg tcgaagttga cggtgtcggc cagtgccgag
     2281 ccgcacagga gacccgtcag gagtgcgaac gccgctaatg ccttcatatc agttttcatt
     2341 acggttttgg tcaggtactc agtgatataa cccttccgcg gttttgcaat acctattacg
     2401 tcggcaagtg aagctatgaa atggattacg cgagagaagg taaaggtggg cagagtagcg
     2461 cgcccctggc gcatccggaa gttcattgac ccggaggcgg agttcattgt ttgcgacgcg
     2521 ctgtatgcgg aatgcgagcg gcgggctggg ccggccgcca
//
LOCUS       DATCGS010000295         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_221322, whole genome shotgun sequence.
ACCESSION   DATCGS010000295 DATCGS010000000
VERSION     DATCGS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221322"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VIL39_06005"
     CDS             complement(<1..607)
                     /locus_tag="VIL39_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481015.1"
                     /translation="MRRLRQDRKSETEGSSHSSFQERVEQAAEAALKRNGSVGPLELF
                     QEMRLLQPVHLEGWRKGNEYYRVLQPWIQVGPEKFQKTIRHFQEWVKQHGLRPIDAAY
                     TRRGPQGIEQLQVTEDGDPEWEKFYRTHYTPADLPEKKTAQLAAKLNRPPELVVFEKV
                     GDEGNCSECGAELAKGAYLLMEKGQPLCLTCADLDELAFLPA"
     gene            complement(612..3236)
                     /locus_tag="VIL39_06010"
     CDS             complement(612..3236)
                     /locus_tag="VIL39_06010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY5481016.1"
                     /translation="MRILVVAKTRRGAGACVGGITEEGHSVRLIASDAASNERAGLEY
                     EVGEVWEIDSAPDPDIVPPHVENIIVLRARRLRRSQKVAETIHRFMPPLAGGPEKLFD
                     GATQATAAGGLYIAERTGLPHRSTMFWAPDQALRLDCEGKRLRYRYPTSDGGRTLTFV
                     GFQEPVPEIPAGTLLRVSLAHWWRPKERPAEELRCFVQLSGWFLETATGSREPLPRTP
                     VPAMTGVSASVETAASPDALEQRALQVLRRTFGFSHFFPVQAEVIVRILQRQDALVVM
                     PTGGGKSLCYQLPALLFEGLTVVISPLVALMQDQVSQLGAVGVSAACLNHMVPLHEHT
                     AITHLIRRGLIKLLYVAPETLLRPETLMLLEQAGLACLAIDEAHCISEWGHDFRPEYR
                     QLQEVRRRFPQAVCLALTATATPRVREDIRSLLGIATEGEFVASFNRPNLFLAVEPRH
                     DALVQVLAFLEQRRGQAGIIYCSTRKQTDELCAALNANGWPALPYHAGLEDTERRHSQ
                     EGFLHDQSPLMVATIAFGMGINKANVRFVVHAHLPKDLESYYQEIGRAGRDGLRADCL
                     LLYSRGDAMVHRHFINQGTECERPGREARLQALMRFAEARDCRRRPLLAYFGETLGQP
                     CGHCDNCVQPAKGEMTDATAAAQKFLACVKLTGQSFGPAHLIAALRGSRAKKVLARRH
                     DRLTVFGIGKEHSTEQWRVLVQQFIRLGLLEQDFEFGSLHLAPKGWDVLHGKEKVLVP
                     RERAPLTATSAAAPAQHDSRAAISPTTRRRCQEIGQLFAAGQSLEHIAKHYNILPETV
                     IQTLCRFHEAGGTLDPERLLAASRLPGPERARVSNAFERLGHGRLAPVFEALSRAVPY
                     QELHLLRLYRLLTLKRYD"
     gene            complement(3233..3751)
                     /locus_tag="VIL39_06015"
     CDS             complement(3233..3751)
                     /locus_tag="VIL39_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY5481017.1"
                     /translation="MKLVTIGVYGFNEAAFFNALQQAGVDTFCDIRWRRGVRGAEYAF
                     ANRARLQQRLAGLGIRYLHFRELAPTPALRQRQTEADKAEGTTKRKRSALGQVFIAGY
                     REERLSPFDSRKFAEQLGPEARTVALFCVEREPAACHRSLLAERLQQDLGAQCEVIHL
                     TPNPVANKGAGG"
     gene            complement(3771..4403)
                     /locus_tag="VIL39_06020"
     CDS             complement(3771..4403)
                     /locus_tag="VIL39_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY5481018.1"
                     /translation="MRYTAVVNGSDQQRAAAPSSQGSRQPLFTIGYGARTLDEFLAAL
                     KTNGIEYLIDVRTAPYSKFKPEFSKDLLQYHVERMGLHYLFMGDTLGGQPKDPACHTD
                     GKVDYDKVRAQPSFQSGIERLRKAFEQQRRAALLCSEGRPEQCHRSKLIGEALAAAGI
                     PVCHIDEDGGLLTQAEVIVRLTKGQMDLFGQASFTSRKRYAPRGDEGRES"
BASE COUNT          838 a         1477 c         1476 g          849 t
ORIGIN      
        1 ccgccggtag aaacgccaat tcgtccagat cggcgcaggt cagacaaaga ggctggcctt
       61 tctccattaa gaggtaagca cctttcgcca actccgcgcc gcactcgctg cagttgccct
      121 cgtcgcccac tttctcgaac accaccagct ccggtggccg gttcagtttg gcggcaagct
      181 gcgcggtctt cttctccggc aaatccgccg gcgtgtaatg cgtgcggtag aacttctccc
      241 attccggatc gccgtcttcg gtgacctgta gttgttcgat gccctgcggc ccacggcggg
      301 tgtaagcggc atcgatgggg cgcaagccgt gctgcttcac ccactcctga aaatggcgaa
      361 tggtcttttg aaacttctcc gggccgacct gaatccacgg ttggagcacg cggtaatact
      421 cgttgccttt gcgccagccc tcaaggtgga ccggctgcag gaggcgcatc tcctggaaca
      481 gttcaagcgg gccgacgctg ccgtttcgct tcagggccgc ctccgccgct tgctcgacgc
      541 gttcctggaa gctgctatgg gaggagccct cagtttcact cttccggtcc tgacgcaggc
      601 gtctcatggg atcaatcata tcgtttgagc gttaataacc gatacaaccg cagcaggtgc
      661 aactcttggt aggggaccgc ccgcgacaaa gcctcgaaaa ccggcgccag gcggccgtgg
      721 ccgagccgct cgaaagcgtt cgacactcgg gcgcgctcag gtcccggcaa gcgcgaagca
      781 gcaagcaggc gttccgggtc gagggttccg ccggcttcgt ggaaacggca gagagtctga
      841 atgaccgtct ccggcaggat gttgtaatgc tttgcgatat gttcgaggct ctgacccgca
      901 gcgaaaagct ggccgatctc ctggcagcgg cgccgcgtgg tcggactaat agcggctctc
      961 gagtcgtgct gcgcaggagc agccgcactt gttgcggtaa gcggcgcccg ctcgcgcgga
     1021 acgagcacct tctctttccc gtggaggaca tcccagcctt tgggcgcgag atgcaggctc
     1081 ccgaattcaa agtcctgttc cagcaaaccc agccggatga actgttgcac caacacgcgc
     1141 cattgttccg tggaatgctc tttgccaatg ccgaagacgg tcaggcggtc gtggcgccgc
     1201 gccagcacct tcttcgcccg cgagccgcgc agcgccgcga tgaggtgcgc cggcccgaag
     1261 ctctggccgg tcagcttcac gcaggcaaga aacttttgcg ccgcagccgt ggcgtcggtc
     1321 atctcgccct ttgcgggttg cacgcagttg tcacagtggc cgcagggctg cccgagcgtc
     1381 tcaccgaagt aggccagcaa cggcctgcgg cggcaatcac gtgcctcagc gaagcgcatc
     1441 agcgcctgga gccgcgcctc ccgcccgggc cgttcgcact cagtgccctg attgatgaaa
     1501 tggcggtgga ccatggcgtc gccgcggctg taaagcagca ggcaatccgc ccgcaggccg
     1561 tccctgccgg cgcggccaat ttcctggtaa tagctctcca ggtccttggg gaggtgagca
     1621 tgaacgacga accggacatt ggccttgttg atgcccatgc cgaaagcaat cgtcgccacc
     1681 atcaacggcg attggtcgtg gagaaagccc tcctggctat ggcggcgctc ggtgtcttcc
     1741 aagccggcat ggtagggcaa ggcgggccaa ccgttcgcgt tgagagcggc gcacagctca
     1801 tcggtctgct tgcgcgtgct gcaatagatg atgccggcct ggccgcgacg ctgctccaga
     1861 aaggcgagca cttggaccaa agcgtcgtgc cgcggctcga cggcgagaaa caggttcggg
     1921 cggttgaagc tggccacaaa ctcaccttcg gtggcaatgc ccagcaggct ccggatgtcc
     1981 tcacgcaccc gcggcgtagc cgtggcggtg agggcgaggc agacggcttg cgggaaacgg
     2041 cggcgcacct cctgaagctg gcgatattcg gggcggaaat cgtggcccca ttccgagatg
     2101 cagtgcgcct catcaatggc caggcacgcc agcccggctt gttcgagcag catgagcgtt
     2161 tccggccgca gcagcgtctc cggcgcgacg tagagcagct tgatgagccc gcggcggata
     2221 agatgggtga tggccgtatg ctcgtgaagg ggcaccatgt ggttcagaca ggcggcggat
     2281 acacccacgg cgccgagctg gctgacctgg tcctgcatca atgccaccag gggagagata
     2341 acgacggtca ggccctcgaa taaaagcgcg ggaagctggt agcagagcga cttgccgccg
     2401 ccggtcggca tcaccaccag cgcgtcctga cgttgtagta tgcgaacgat gacctcggcc
     2461 tggaccggga agaagtgcga gaaaccaaag gtccgcctca gcacctggag agcgcgctgt
     2521 tcgagcgcgt caggggaagc agcggtttca acggaggccg atacgccagt catcgctggc
     2581 accggcgttc gcggcaaagg ttcccgggag ccagttgcgg tttcaaggaa ccacccggac
     2641 aactgcacaa aacagcgcaa ttcctccgcc ggccgttcct tgggccgcca ccagtgagcc
     2701 agggaaacgc gcagcagcgt gccggcagga atctcgggga ccggctcctg aaaaccgaca
     2761 aaggtcagcg tgcgcccgcc gtcgctcgtg gggtagcgat agcggagccg cttgccctcg
     2821 caatcgagcc ggagcgcttg gtccggcgcc cagaacatcg tgctgcgatg tggcaagccg
     2881 gtgcgctcgg cgatataaag cccgcccgcc gccgtggctt gcgtggctcc atcgaaaagc
     2941 ttctccggcc cgcccgccaa cggcggcata aagcggtgga tggtttccgc caccttttgc
     3001 gagcgccgga ggcggcgggc gcgcagcacg ataatgttct ccacatgcgg tggaacgatg
     3061 tccgggtccg gcgccgaatc aatttcccac acttcgccga cctcgtattc gaggcccgcg
     3121 cgctcgttgc tggccgcgtc gctggcgatc agccgaacgc tgtgtccctc ttcggtgatg
     3181 ccgccgacac atgcgccggc gcctcgccgg gtcttggcca cgacgagaat cctcatccgc
     3241 ccgctccctt attggcaacc ggatttggcg tcaggtgaat aacctcgcat tgtgcgccca
     3301 ggtcctgctg caaccgctcg gccagaagcg agcggtggca tgcggcgggc tcgcgttcca
     3361 cgcagaagag tgccaccgtc cgcgcttcgg gtcccagttg ttcggcgaac ttccgactat
     3421 caaagggcga aagccgttcc tcgcggtacc cggcgatgaa cacctggccc agggccgagc
     3481 gtttgcgttt ggtggtgcct tccgccttgt cggcctcggt ctggcgctgg cgcaaggccg
     3541 gggtcggcgc caactcgcgg aagtggaggt aacggatgcc aagtccggcg agccgttgct
     3601 gaagccgggc gcggttagcg aaggcgtatt ccgccccgcg cacgccgcgc cgccagcgaa
     3661 tgtcgcagaa cgtgtccacg cccgcctgtt gcagcgcatt aaagaacgcc gcctcgttga
     3721 aaccataaac gccaatcgta acgagtttca tccccaatca gattctctgt tcagctttcc
     3781 cggccttcat ccccccgcgg ggcgtaacgc ttccgggagg taaaggaagc ttgcccgaac
     3841 aggtccattt gccccttcgt caaccggacg atcacctccg cctgcgtgag caagccgccg
     3901 tcctcgtcta tgtggcagac gggaatgccg gcggcggcca gcgcctcgcc gatcagtttg
     3961 ctgcgatggc attgctccgg ccggccctcg ctgcacagca acgctgcgcg acgctgctgc
     4021 tcgaaggctt ttcggagccg ctcgattcca ctttggaagg atggctgggc gcggaccttg
     4081 tcgtaatcca ccttgccgtc agtgtggcaa gcggggtcct tgggctggcc accgagcgtg
     4141 tcgcccatga acaggtagtg taggcccatg cgctcgacat gatattggag caggtctttc
     4201 gagaactccg gtttgaattt cgagtaaggc gccgtgcgga catcaataag gtactcgatg
     4261 ccgttcgtct tgagcgccgc caggaactca tccagcgtgc gcgcgccgta gccaatcgtg
     4321 aaaagcggct gccggctgcc ctggctcgaa ggcgccgccg cccgctgctg gtcactgcca
     4381 ttcacgacag cagtgtatcg catttagacg gcttgggaag atattgtcgt ccaagtcagg
     4441 tattacgccc cgggcgtccg ctcatccatc atgcgaaaac catgcgaagc ccatgcgaag
     4501 cccaggcgaa caacacgctg cttacacgct accactaggc tgggtgctgg ttggcagcag
     4561 caattctcat tatggccaga gttcggggaa gacaggtggt cgcgcccagg ctttggggta
     4621 gctttggggt agctttgagg
//
LOCUS       DATCGS010000296         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_620300, whole genome shotgun sequence.
ACCESSION   DATCGS010000296 DATCGS010000000
VERSION     DATCGS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620300"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..526
                     /locus_tag="VIL39_06025"
     CDS             215..526
                     /locus_tag="VIL39_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY5481019.1"
                     /translation="MTTHALFRLPALIILLAPALLHAAEAPPAPTWHAQSLGQALLYM
                     LLFAGIGIVAAIAGYKIFDKCTPGDLTKEILEHRNVAAAIVAAAVILGVCIIIAAAML
                     G"
     gene            542..910
                     /locus_tag="VIL39_06030"
     CDS             542..910
                     /locus_tag="VIL39_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481020.1"
                     /translation="MKRSRSIRLVLLGSLSAGALAGCGPGQPPPLSSEAVYTNNCYVP
                     GVGYYHAPFRAWYPLPYNHFDPQTQRYFYGGQWSASACESITNISAPAAGIVQHAEAQ
                     RTDVVRGGFGSSSRSHYIHS"
     gene            910..2031
                     /locus_tag="VIL39_06035"
     CDS             910..2031
                     /locus_tag="VIL39_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006617954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEY5481021.1"
                     /translation="MQRHRSIPRPRWREKVEEIGLTYHSHEDGPYWDESAAYELSAKE
                     VDALEAAANALHYLCIEAAEAVIKNEWWPRLGIPEIAIPSILGSWERDDFSLYGRFDL
                     SFDGRTPPKLLEYNADTPTALVEAAVAQWFWLQDLHPGADQFNSIHERLIAAWARCPA
                     TTIHFSSIKENLEDEQTVLYLRDTCEQAGRQTRPVYVEDVGWDRRQDCFVDLENRRIE
                     YCFKLYPWEWLWHEEFGPHLAKDCVRFIEPAWKMLLSNKGLLPILWELFPDHPNLLPA
                     YELAAPLGGRYVRKPKLSREGSNVTWVEGGVVIEETSGDYGHEGHVYQAPAAMPEFDG
                     QRPVFGVWVIDHEAAGLGIREDTRRITGNLSRFVPHYFQ"
     gene            2054..>2270
                     /locus_tag="VIL39_06040"
     CDS             2054..>2270
                     /locus_tag="VIL39_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY5481022.1"
                     /translation="MSYNNPTPIRIGMTGNLSGKQYRVVGRVVLGEEENGETYYWNEF
                     NLESDGDDEATLVYEETETAGEWRLFHL"
BASE COUNT          435 a          706 c          708 g          421 t
ORIGIN      
        1 ttgtggcgat ctatttgccg ctgcttgccg cgctcaacag cctggcgcaa aagtgagcta
       61 aacgggattg gaacgcggac gggtgcatcg ctggcgactt gctctgtgca ggctggagtg
      121 atagaagctg gttcatgaat atccattggc tggtggacgt gtcggctgtg ctgcgcgtcc
      181 gccagcattc aggccatcca ttagtgtatt gtttatgaca acccatgctc tattccggct
      241 tccggcctta atcatccttc tcgccccagc cttgctgcac gcggcggaag cgccgccggc
      301 tcccacctgg cacgcccaat ccctgggtca ggcgctcctc tatatgctgc tcttcgcggg
      361 gatcggcatc gtggcggcga ttgccggcta caagattttc gacaaatgca cgcccggcga
      421 tcttaccaag gaaatcctgg agcaccgcaa cgtcgcggcg gcgatcgtcg cggcagcggt
      481 gatcctcggg gtgtgcatca ttatcgccgc cgcgatgctg ggctgaaggc gttcagcgcc
      541 cgtgaaacgc agtcggagca tacgcctggt gttgctcggc agcttgtctg ccggagcgct
      601 ggccggctgc gggcctggcc agccgccgcc gctcagttcg gaggccgtct ataccaataa
      661 ctgttacgtg cctggcgtgg gctactatca cgcgccgttc cgggcgtggt atcccctgcc
      721 ctacaatcat tttgatccgc aaacgcagcg gtatttttat gggggccaat ggtcggcgag
      781 cgcttgtgag agcatcacga acatctccgc gccggccgcc ggcatcgttc agcacgccga
      841 ggcgcaacgc acagatgtcg tgcgcggcgg ctttggcagc agctcgaggt cgcattacat
      901 ccattcgtga tgcaacggca tcgttccatt cccaggcccc gctggcgcga gaaagtcgag
      961 gagattggcc tcacttatca ttcacatgaa gatgggcctt actgggacga atccgccgct
     1021 tacgagctga gcgcgaaaga agtggatgcg ctcgaagcag ccgccaacgc gctgcattac
     1081 ctgtgcatcg aagcagccga ggcggtgatc aagaatgagt ggtggccccg actgggcatc
     1141 cccgagatcg ccatccccag cattctcggc tcctgggaac gcgacgattt cagcctctac
     1201 ggacggtttg acctgagctt cgacgggcgc acaccgccca aactgttgga atacaacgcc
     1261 gacacgccga cggccctggt ggaggcagcg gtggcgcagt ggttctggct gcaagacctc
     1321 caccccggcg ccgaccagtt caactcgatt cacgagcggc tcattgcggc gtgggcacgc
     1381 tgcccggcca cgaccatcca cttcagcagc atcaaggaga acctggagga cgaacagacg
     1441 gtgctttatc tgcgggacac ctgcgagcaa gccggccgcc agacccggcc ggtctatgtc
     1501 gaggacgtag gttgggaccg gcgccaggat tgtttcgtgg acctggagaa ccgccgcatc
     1561 gaatactgct tcaagctcta cccctgggaa tggctgtggc acgaggaatt cggaccgcac
     1621 ctcgcgaagg actgcgtgcg attcatcgag cccgcctgga agatgctgct gagcaacaag
     1681 ggattgctgc ccatcctgtg ggaactcttt ccggatcacc cgaacctcct gcccgcctac
     1741 gaattggccg cgccgctggg tggtcgctac gtccgcaagc ccaaactcag ccgcgagggc
     1801 agcaacgtaa cgtgggtcga aggcggcgtg gtgatcgagg aaaccagcgg cgattacggc
     1861 cacgagggac acgtctatca ggccccggcg gccatgcccg agttcgacgg acagcgcccg
     1921 gtcttcggcg tctgggtgat tgaccacgag gcggccggcc tgggcatccg cgaggacacc
     1981 cgccggatca cgggcaacct gagccggttc gtgccgcatt atttccagtg agacacgcca
     2041 accgccccag gccatgagct acaacaaccc cacacccatc cgcatcggca tgaccggaaa
     2101 cctttccggc aagcagtatc gcgttgtggg ccgggtggtg ctgggcgagg aggagaacgg
     2161 cgaaacctat tattggaacg agttcaatct ggaaagcgac ggcgacgacg aggccacgct
     2221 ggtctatgag gaaaccgaga ctgccggcga atggcggctg ttccacctgt
//
LOCUS       DATCGS010000297         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_125960, whole genome shotgun sequence.
ACCESSION   DATCGS010000297 DATCGS010000000
VERSION     DATCGS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125960"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="pglZ"
                     /locus_tag="VIL39_06045"
     CDS             complement(<1..1078)
                     /gene="pglZ"
                     /locus_tag="VIL39_06045"
                     /inference="COORDINATES: protein motif:HMM:NF033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HEY5481023.1"
                     /translation="MSETATTLLDALQEALQRAAQYNHDDQVAPAAILWPDKERQWAA
                     LIRELRPRLPLLTFGDYSPADRCGPAPYIRCMIGRTLSEDRLPDDATPIIYLPGFSRQ
                     DLRAIEECPRDLQPLAGLQYSGVLWTHKNGRDWTIAGFLQSADGGLGIPVAADKATRE
                     ALSRALPVLAREPLSHLRKEAPLRAPFLDGLLNPDEARRLLLWMNDPKGYRAGVSQPE
                     WTAFCNLCQHKYGIHPQQDGELAAAARLGQPKGAWEGVWQRFLEAPDAYPKVPELLRR
                     ARPAQLTLFEKKLPYWPQDNESAETELRESLLALKDRLPAESRTAINELEARHGPRRG
                     WVWAKLEAAPLAEALVHLLKLAQLS"
     gene            complement(1153..1557)
                     /locus_tag="VIL39_06050"
     CDS             complement(1153..1557)
                     /locus_tag="VIL39_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5481024.1"
                     /translation="MGLKELQDELARLHRVCIDTAVFAYHLAEHPRYVEYTSAILAAV
                     EQKKLEGVTTTITLTELLTYPAQKGDAETMREWELYLTNFPNLLIIPLDQPLAREAAR
                     VRAETRLKTPDAVQVAGRIGIRTPACTEPMRS"
     gene            complement(1548..1709)
                     /locus_tag="VIL39_06055"
     CDS             complement(1548..1709)
                     /locus_tag="VIL39_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481025.1"
                     /translation="MKLALSLFVLQGNTVLLRARPASFTNALRGLHKEVWEGEDIGQW
                     LAGEREAWD"
     gene            complement(1832..3016)
                     /locus_tag="VIL39_06060"
     CDS             complement(1832..3016)
                     /locus_tag="VIL39_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY5481026.1"
                     /translation="MTYQHPDFTLPAELLEQIAAQGFDALPELIRIVINEAMRMERQN
                     HLGVAPYQRSPERRGYGNGFKDHTERTRLGKITFAIPQVREGGFFPQALEKGLRSERA
                     LTLALAEMYVQGVSTRKVTAIVEELCGSSVSSSQVSRAAGLLDEKLEAWRQRPLGEMV
                     YLYLDARYEKVRQDGQIRDAAVLIATAVDPTGHRQVLGVSVALSEQEVHWRTFLQSLV
                     ARGLRGLQLIISDDHAGLKAARQAVFGGVPWQRCQFHLQQNAQAYVPRKEMQAEVAAD
                     IRMIFNAPNRTTANEYLARTVQKYAKSAAKLADWLERSIPEGLAVFAFPTAHQRRLRT
                     SNSLERVNRELDRRTTVVSIFPNEAACLRLISAVLIELDEEWQSDRIYLSFETAGTPS
                     SS"
     gene            3002..>3249
                     /locus_tag="VIL39_06065"
     CDS             3002..>3249
                     /locus_tag="VIL39_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481027.1"
                     /translation="MLVGHGVPPSVGLVLAERIHQTYRFPAAAKPFYRKNDTLFNQPQ
                     QAKMRARMLQDAALASAAADIKEAEEAERPVCPKCGARL"
BASE COUNT          561 a         1006 c         1045 g          637 t
ORIGIN      
        1 cgctcaactg cgccagcttg aggagatgga ccagcgcttc cgccaggggc gccgcctcca
       61 gtttggccca cacccagccg cggcgcggcc catgcctggc ctccagctcg ttgatcgccg
      121 tccgggactc ggccggcaat ctgtccttga gcgccagcag cgattcacgt aactccgtct
      181 cagccgattc gttatcttgc ggccaataag gcagtttctt ttcgaagagc gtcaattgcg
      241 ccgggcgtgc gcggcgcagc agctcgggca ccttgggata agcatccggc gcttccagga
      301 agcgctgcca gacaccctcc caggctccct tgggctgacc gagccgcgcc gccgcagcca
      361 gctcgccgtc ctgttgcggg tggatgccat acttgtgctg gcacaggtta cagaaggcgg
      421 tccactcggg ctggctgacc cccgcccggt agcccttcgg atcgttcatc catagcagca
      481 gccggcgggc ttcatcagga ttgagcaggc cgtccagaaa aggagcccgt aacggagctt
      541 ccttgcgcag atggctaagc ggctcgcgcg ccaggacggg cagggcacgc gacagcgcct
      601 cgcgcgtggc tttatcggct gcgaccggaa tgccgaggcc accatcggcg ctttgcagga
      661 atcccgcgat agtccaatcg cggccgttct tgtgggtcca gagcacgccg ctgtattgca
      721 ggccggccag gggctgaagg tcccgcgggc actcctcgat ggcgcggaga tcctggcggc
      781 tgaagccggg caggtagatg atgggcgtag cgtcgtccgg taaccggtct tcagacaggg
      841 tcctgccgat catgcagcgg atgtagggcg cagggccgca gcggtcggcg gggctgtagt
      901 ctccaaaggt aagcaacggc agtcgcggcc gcaattcccg gatgagagcg gcccattgac
      961 gttccttgtc gggccagaga atcgccgcag gcgcgacctg gtcgtcgtgg ttgtattgcg
     1021 ctgcccgttg cagggcttcc tgcagcgcgt caagcagcgt cgtcgcggtc tcgctcatgg
     1081 ctcagctcac gaaatcgtcc aggatgagga cacgcggaga actaacgcgc ttgtcccagg
     1141 gccggtcatt ggtcaggatc gcatcggctc cgtgcaagcg ggcgtgcgga ttccgatccg
     1201 cccagctacc tgaaccgcgt caggggtttt cagccgcgtt tcagcgcgca cgcgggctgc
     1261 ctcacgggcc agcggctgat ccaggggaat gataagcagg ttcgggaagt ttgtcaggta
     1321 caattcccac tcgcgcatcg tctccgcatc gcctttttgc gcggggtatg tgagcagctc
     1381 cgtcagcgtg atcgtggtgg tcacgccttc cagtttcttt tgctccaccg cggcgagaat
     1441 ggccgacgtg tactccacgt agcggggatg ctccgccaga tggtaggcaa agacggccgt
     1501 atcaatgcag accctgtgca gacgcgctaa ctcgtcttgc agctccttca gtcccatgcc
     1561 tctcgctccc ctgccagcca ttggccgatg tcctcgccct cccagacttc cttatggagg
     1621 ccgcgtagag cattggtgaa gctggccggt cgggcgcgca ggaggacggt gttgccctgc
     1681 aacacgaaaa gcgatagtgc aagtttcatt ctgtaaattc gctggtgacc tttcggttct
     1741 ttaaggtgct gatgcaagcc cggctgtgga caagtggaca actgcgaaag tgccgcggtt
     1801 gcccacttgc ccacagcctc tactactact actacgacga cgacggcgtg ccggctgttt
     1861 cgaaggacag gtagatgcgg tcgctctgcc attcttcgtc cagttcgatc aggaccgcac
     1921 tgatcagacg caggcaggcg gcctcgttcg ggaagatgct aaccaccgtg gtgcggcggt
     1981 cgagttcccg attgacccgt tccaggctgt tgctggtgcg cagccggcgc tgatgggccg
     2041 ttgggaaggc gaagacggcc aagccttccg ggatgctcct ttccaaccaa tcggccagct
     2101 tggcagcgct cttggcgtat ttctgcaccg tgcgggcgag atactcgttg gcggtggtcc
     2161 gatttggtgc gttgaaaatc atgcggatgt cggcggcgac ttccgcctgc atctctttgc
     2221 gcggcacata ggcctgggcg ttctgttgca gatggaattg gcagcgctgc cacggcacgc
     2281 cgccgaagac cgcctggcga gccgccttga ggccggcgtg gtcatctgag ataatcagtt
     2341 gcaggccccg caggccacgc gccaccaggc tttgcaggaa ggtgcgccaa tgcacttcct
     2401 gctcgctcaa ggccactgag acgcccagca cctgccggtg gcctgtcggg tccactgccg
     2461 tggcgatcag cacggctgca tcgcggatct ggccgtcctg acgtactttt tcgtagcgcg
     2521 catccaggta caggtagacc atctcgccca gtggtcgctg gcgccaggct tcgagcttct
     2581 catccagcag ccccgcggct cggctgacct ggctggatga gacagagctg ccgcacagct
     2641 cttcgacgat ggcggtcacc ttgcgggtgg agacgccctg cacgtacatc tcggccagcg
     2701 ctagggtcag cgcccgttcg ctgcgcagcc ctttctccaa tgcctgcggg aagaaaccac
     2761 cctcccgcac ttggggaatg gcaaaggtga tcttgcccag acgcgtacgc tcggtgtggt
     2821 ccttgaagcc gttgccgtaa ccgcgccgct caggcgaacg ttggtaaggt gcaacgccca
     2881 ggtggttctg gcgttccatg cgcatcgctt cgttgatcac gatgcggatc agctcgggca
     2941 gggcatcgaa accctgtgcg gcgatttgtt ccagtaactc ggccggtagg gtaaaatcgg
     3001 gatgctggta ggtcatggtg tgcctccttc ggttggattg gttcttgccg aaaggataca
     3061 ccagacctac cgcttccccg cagcagctaa gcccttttac agaaagaacg atacactatt
     3121 caaccaacca caacaggcga agatgcgggc gcggatgttg caggatgcgg cgttggcgag
     3181 tgcagcggcc gatatcaagg aggcggagga ggcggaacgg ccggtgtgcc ccaaatgcgg
     3241 ggcgaggct
//
LOCUS       DATCGS010000298         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_126104, whole genome shotgun sequence.
ACCESSION   DATCGS010000298 DATCGS010000000
VERSION     DATCGS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126104"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VIL39_06070"
     CDS             <1..590
                     /locus_tag="VIL39_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY5481028.1"
                     /translation="LNVPLRLVGVVGKLYVVLESDRGLVLLDQHAAHERILFEQMLNR
                     LERNDQAPSQRLLLPETVELSARDASFLREQLPMLTRLGVGLSEFGERTFLLDALPPF
                     VKAPDARRFVLELVDELKAAGRELNSLRLGEHTVAKTVCRHAVKANDPLAGRELESLV
                     EDLRHCAMPYTCPHGRPTLIEMNFRELEKKFGRTQ"
     gene            complement(600..1946)
                     /gene="tig"
                     /locus_tag="VIL39_06075"
     CDS             complement(600..1946)
                     /gene="tig"
                     /locus_tag="VIL39_06075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY5481029.1"
                     /translation="MNVTVENLAPCKKLMRVEVEAQKVDETFESVTKDFRREANLPGF
                     RPGKAPRDMVLRKYEKEILEETKRKLISEAYRKAVEEQKLDVVSHPDIEEIQFGRGLP
                     LQFAATIETAPEFELPEYKGLQIKREMQSVTEADMERAINLLRERQVSFTKVERPVQS
                     ADIAVVNYTGTCEGKPITELAPAAKGLTEQKHFWVEVGANSFIPGFGDQLIGAKAGDK
                     RTVTVDFPADFVTPQLAGKKGSYEVEVVEVKEKVLPALDEALAKAYGAESLEKLRAGV
                     RSDLENELKFKQEKTLRSELVRALLGRINFDLPETAVARETRNVVYDLVQENTKRGIP
                     RQAIEQQKEQIYSAATHNAKERLKVQFLLQKIAEKEDIKVSQEEIAQRVHHLAGMYQI
                     PADKFLRDLQKRNGLIEVYDQIMNVKVIDFLQQNAKIEDVPPSSFVAPEAPAVNPS"
     gene            complement(2103..>2834)
                     /locus_tag="VIL39_06080"
     CDS             complement(2103..>2834)
                     /locus_tag="VIL39_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481030.1"
                     /translation="AIGAGWDETGNKDGKVLQFAAPGDAAGLGQRCLRVEAHLAKDTG
                     GGLTKWFEPADPIFIRFYTRFDPGCDYVHHFVTLRANKGLRGGDKWSGFGGAGLKPEG
                     IERFSTAIEPWGNWGRWPAPGRWNFYSYWHEMEISKDGKYWGNSFGVPTAPVIPRGRW
                     ICVEFMLKHNTPGQPDGEQAFWIDGALQGHWKGINWRKAASLKANALTLESYITDRWT
                     TNPVNVVFFDNVVLARRYIGPVGRK"
BASE COUNT          456 a          928 c          862 g          588 t
ORIGIN      
        1 tgctcaacgt cccgctgcgg ttggtcggcg tggtcggcaa gctctacgtg gtgctggaat
       61 ctgaccgggg cctggtgctg ctcgaccagc acgcggcgca cgagcgcatt ctgtttgagc
      121 agatgctcaa tcggctggag cggaacgacc aggcaccctc ccagaggctg ctgctgccgg
      181 agaccgtcga gctgtcggcg cgggatgcga gcttcctgcg ggagcagttg ccgatgctga
      241 cgcggctggg cgttgggttg agtgaattcg gcgagcgcac cttcctgctc gatgccctgc
      301 cgccgtttgt aaaagccccg gacgcccgcc ggttcgtgct cgaactggtg gacgaactca
      361 aagccgcggg gcgggaactc aattccctgc ggctgggcga gcataccgtg gccaaaaccg
      421 tctgccgtca tgcggtgaaa gccaacgacc cgctggccgg gcgggaactg gaaagcctgg
      481 tcgaggacct gcgccactgt gcgatgccct acacctgccc gcatggccgg cccacgctca
      541 tcgagatgaa cttccgcgag ctggaaaaga agttcgggcg tacccaatag cccgagcgct
      601 cagctcggat tcaccgccgg cgcttcgggc gctacaaacg agctgggcgg cacgtcctca
      661 atcttcgcgt tctgctggag gaaatcaatg accttcacgt tcatgatctg gtcatatact
      721 tcgatcaggc cattgcgctt ttgcaggtct ctgaggaatt tgtccgccgg tatctggtac
      781 atcccggcca ggtggtgaac gcgctgggcg atttcctcct gggaaacctt gatgtcttcc
      841 ttctcggcga tcttctgcag caggaactgg actttgaggc gctctttggc gttgtgcgtc
      901 gcggccgaat agatttgctc cttctgctgc tcgatcgcct ggcgggggat gccgcgcttg
      961 gtgttttcct gcaccaggtc atagaccacg ttgcgggtct cccgggcgac cgcggtttcc
     1021 ggcagatcaa agttgatccg gcccagcagg gcgcgcacca actcgctgcg cagggtcttt
     1081 tcctgcttaa acttcagctc gttctccagg tcgctgcgca cgccggcccg gagtttctcc
     1141 agactctcgg ccccataagc tttggccagc gcctcatcca gcgccggcag cactttctcc
     1201 ttcacctcga ccacttcgac ttcgtaactg cctttcttgc ccgcgagctg gggcgttacg
     1261 aagtcggccg ggaagtccac tgtaaccgtc cgcttgtcgc ccgccttggc gccgatgagc
     1321 tggtcgccaa agccggggat gaaggaattc gcgccaactt ccacccagaa atgcttctgc
     1381 tcggtcagcc ctttggccgc gggggcgagt tcggtaatcg gcttgccctc gcaggtgccc
     1441 gtgtagttca cgacggcgat gtcggcgctc tggaccgggc gctcgacctt cgtgaagctc
     1501 acctgccgct cgcgcagcag gttgatggct cgctccatat ccgcctcggt cacgctctgc
     1561 atctcgcgct tgatctgcaa gcccttgtat tcgggcagct cgaattccgg ggccgtctcg
     1621 atcgtggccg cgaactgcaa gggcagcccc cggccgaact ggatctcctc gatgtcgggg
     1681 tggctcacca catccagttt ctgctcctcg acggccttcc gataggcctc ggagatgagc
     1741 ttgcgcttgg tctcctccag gatctccttc tcgtatttcc gcagcaccat gtcgcgcggc
     1801 gccttgcccg gccggaaacc cggcaggttg gcctctcggc ggaaatcctt cgtgactgat
     1861 tcgaaggttt catccacctt ctgcgcttca acttccacgc gcatcagctt tttacatggg
     1921 gccaaatttt ctaccgttac attcacaaat aaacctttcc gttagtcgct gacaaagtcc
     1981 aatgggcgag agggttttgt gtttcccccg ctcagcgagc cgggaacggt aggcgagccg
     2041 gggtggcgcg tcaagcctgc cttcgggctc tgccttcggc cagacgagcg gagaggatgg
     2101 ggtcacttcc gcccgaccgg gccgatgtag cggcgcgcga gaacgacatt gtcgaagaac
     2161 accacgttta ccgggttcgt cgtccagcgg tcggtgatgt agctttcgag cgtcaacgcg
     2221 ttggccttga gcgacgcggc cttgcgccag ttgatgccct tccagtggcc ttgcagcgcg
     2281 ccatcaatcc agaacgcctg ctcgccgtcc ggctggcccg gcgtgttgtg cttgagcatg
     2341 aactcgacgc aaatccaccg gccgcgcggg atgaccggag cggtgggcac gccgaaggaa
     2401 ttgccccaat acttcccgtc cttcgatatc tccatctcgt gccagtagct gtagaagttc
     2461 cagcgccccg gcgccggcca gcggccccag ttgccccacg gctcgatggc ggtggaaaag
     2521 cgctcgatgc cttcgggctt caaccccgcg ccgccaaacc cgctccactt gtcgccgccc
     2581 cgcaggccct tgttcgcgcg cagcgtcacg aagtgatgca cgtaatcgca gcccggatcg
     2641 aaccgggtgt agaagcggat gaagatcggg tccgccggct cgaaccactt ggtgagaccg
     2701 ccgcccgtgt ccttggcgag atgcgcctcg acgcgcagac agcgttggcc aagcccggcg
     2761 gcgtcacctg gcgcggcaaa ctgcaacacc ttgccgtcct tgttgcccgt ctcgtcccag
     2821 ccggcgccga tggc
//
LOCUS       DATCGS010000299         3790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_371868, whole genome shotgun sequence.
ACCESSION   DATCGS010000299 DATCGS010000000
VERSION     DATCGS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371868"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VIL39_06085"
     CDS             <1..912
                     /locus_tag="VIL39_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5481031.1"
                     /translation="HHASRITHPSSFYRVGLWRLGLAIVRILPAWLVKRFCVTVAELH
                     FLFGRQRREVVVQNLLPALSGNRSEAEQIARRLYRNFASKLVDLWRVEGGVPVRDWLT
                     DPAGLEIIRAARQRGRGILFITLHLGNWEHGGLLLTEVGIQLTVLTLAEPDDELTKLR
                     MASRERWKIQTLIIGQDSFAFLEVIKRLQAGAALAISLDRPPQRSGVPVELFGHPFEA
                     SAGAAELARASGCALIGVTIVRRRAGFAVKVLPEFTYDRQALGNREARRQLTQQILRA
                     FEPQIREDIDQWYQFVPIWPNKALVQE"
     gene            951..1583
                     /locus_tag="VIL39_06090"
     CDS             951..1583
                     /locus_tag="VIL39_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY5481032.1"
                     /translation="MVSVCHAGDPSERFDRWFAVQTNLQSWSADFTQTRSLKVLAQPL
                     VATGKVWVTTPGLFRWELGQPAQTIALRQPNQLLIIYPRLKRAEKYALSAAPTGPLKD
                     ALALMDATLPRDRATMEERFRLLSAAETNSLLQMTLQPKSASARKFISEILIAFRTND
                     FAIAVTELKFADGSSLRNDFTNTVLNRPMDPALFDVKLGPDFTVVEPLRQ"
     gene            1580..2194
                     /locus_tag="VIL39_06095"
     CDS             1580..2194
                     /locus_tag="VIL39_06095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEY5481033.1"
                     /translation="MTAKPRSLQEALKLLPHGPEFRFLDRLLSLVPGKEAVGEYRVRG
                     DEYYLRGHLPGEPIFPGVLLIEAAAQLAGVLAQNDPVIPSLPGLKLTALRAVKILGSA
                     RPGQTVRLEAAITGRLGPLVQAKASAYVANTLVMHAELTLSGTAEDVEGGRAGVRPSP
                     GAATSANKGASQCSNVPGGSEPAAPGDGRTPEVRPKCAPGSARS"
     gene            2321..2944
                     /locus_tag="VIL39_06100"
     CDS             2321..2944
                     /locus_tag="VIL39_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481034.1"
                     /translation="MTVAWNHPPPTYSHRCFMVVKSARQFLYHARFEPGLPIAEPEVC
                     RRLVRQVLSRNPRRPSAAPDRIVIPGYEGLRALTQAHEPLLKAECGAPWQSYFLRSHW
                     RMVFPIWKRHQERIAQQLRQRVSQGSAPTVHIFRFPRITINHGLMLFGMVESERDIQF
                     EAYDPNLTDHPAKLIYDRVRRSFSFPPTCYWPGGELSIIEIYRGGLY"
     gene            3073..3279
                     /locus_tag="VIL39_06105"
     CDS             3073..3279
                     /locus_tag="VIL39_06105"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEY5481035.1"
                     /translation="MSMAELIYSKAKTLPDSAQVALLQLVELLAKDPSTKPLARIEPG
                     SARGLVSMAEDFDAPLVDFEPYMK"
     gene            3276..3662
                     /locus_tag="VIL39_06110"
     CDS             3276..3662
                     /locus_tag="VIL39_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5481036.1"
                     /translation="MNLLLDTHAFLWFIASDPRLSNQAQSLIQTPANRRLLSMASLWE
                     MAIKVSLGKLTLAQPFDQFIPRQLQLNQIEVLAIELSHLAAVVAMPFHHRDPFDRLMA
                     AQCQIEGLPIVSNDPVFDAYSVRRVW"
BASE COUNT          691 a         1211 c         1137 g          751 t
ORIGIN      
        1 catcacgcat cacgtatcac gcatccctcc tccttttatc gcgtcggcct gtggcgactt
       61 gggttggcca tcgtgcgaat cctgccggcc tggttggtga agagattctg cgtgaccgta
      121 gccgagttgc acttcctgtt cgggcggcag cgccgtgagg tggtggtgca gaacctgctc
      181 cccgcactct ccggcaaccg gtcggaggcg gagcaaatcg cccgccggct ctatcggaac
      241 ttcgcttcga agctggtgga tttatggcgc gtcgagggcg gggtgccggt gcgagactgg
      301 ttgactgacc ccgccggact ggaaatcatt cgcgccgccc gccaacgcgg gcgcggcatc
      361 ctttttatca cgctccacct gggcaattgg gagcatggcg gactgctgct gaccgaggtg
      421 ggcattcagt taaccgtgct gacgctggcg gagcctgacg atgagctgac caagctgcgg
      481 atggcctcgc gcgaacgctg gaagatccag accctgatca tcggccagga cagcttcgcc
      541 tttctggagg tcatcaaacg acttcaggcg ggagcggccc tggcaatctc gcttgatcgg
      601 ccaccgcagc ggagtggcgt gccagtcgag ctgttcggcc acccgttcga agcatcggca
      661 ggcgcggcgg aattggcgcg ggcgtcgggc tgcgcgttga tcggggtaac catcgttcgc
      721 cggcgcgcgg gcttcgccgt gaaagtgctc ccggagttca cctatgatcg ccaggcgctg
      781 ggcaatcgcg aagcccgccg gcaactgacc cagcaaatcc tgcgtgcatt cgagccgcag
      841 attcgcgaag atatagacca atggtaccaa ttcgttccta tctggccaaa caaagcactc
      901 gtgcaagaat agacgggctg cggcgctgct tttggattgc gttgtgtgcg gtggtatccg
      961 tttgtcacgc gggtgacccc agcgagcggt tcgaccgatg gttcgccgtg cagaccaacc
     1021 ttcagagttg gtccgccgat ttcactcaga cccgctcgct caaggtgctc gcccagccgc
     1081 tggtcgccac cggcaaggtc tgggtgacga cgccgggcct gttccgctgg gaactgggcc
     1141 aaccggcgca gaccattgca ttgcgccagc ccaaccaact gctcattatc tacccgcgcc
     1201 tcaagcgggc ggagaaatac gctctcagcg ccgcgccgac gggtccattg aaggacgcgc
     1261 tggccctgat ggacgccacc ctccctcgcg atcgggccac catggaagag cgctttcgcc
     1321 tgctctcggc ggcggaaaca aattccctgc tgcaaatgac gctccagccc aagagcgcct
     1381 ccgcccgcaa attcatcagt gagattctga ttgcctttcg caccaacgat ttcgccatcg
     1441 cagtcaccga gctgaagttt gccgacggct cgagcctgcg caatgatttc accaacacgg
     1501 tgctcaaccg gccaatggac ccggcgctgt tcgacgtgaa gctcgggcct gatttcaccg
     1561 tggtggagcc gctgcgccaa tgaccgcgaa gcccaggtca ttgcaggagg ccctcaagct
     1621 gctgccgcac ggcccggaat tccggttcct ggatcggttg ctgagcctgg tcccgggcaa
     1681 ggaggccgtc ggcgagtatc gggttcgcgg cgacgagtat tacctgcgcg gccatctgcc
     1741 cggtgagccg atcttccccg gcgtgctcct gattgaagcc gccgcgcaac tggccggtgt
     1801 tttagcgcag aatgatccgg tcattccatc cttgcccggc ttgaaactca ccgcgctgcg
     1861 cgcggtcaag attctcggct cagcccggcc cgggcaaacg gtccggctcg aagctgctat
     1921 tacaggccgt ctgggtcctt tggtccaggc caaggcttca gcctacgtcg cgaacaccct
     1981 ggtaatgcac gccgagctta ccttgagcgg cacggccgag gacgtggaag gcggcagggc
     2041 cggagtgcgc ccgtccccgg gcgcagcgac ctccgcaaac aaaggggctt cgcaatgttc
     2101 taacgtgccg ggtgggtcgg agcctgctgc gcccggggac gggcgcactc cggaggtgcg
     2161 gccaaaatgc gcccccggct ctgcgcgatc ctaagaaacg cttgtgctaa cgggtccgca
     2221 accagaccac tgcgcccgcc cctttcggtt cgagagggac acttttgctt tcgccaacga
     2281 gttgatttgg caataccgct tcgacccggt gacgaaggca atgactgttg cctggaatca
     2341 tccgcccccc acctattccc atcgctgctt catggtggtc aaatcggccc ggcagttcct
     2401 ttaccatgcc cggttcgaac cgggcctgcc gattgcggag ccggaggtct gtcgccggct
     2461 tgtccgtcaa gtcctctcac gcaatcctcg ccgaccgagc gcagcgccgg atcgaatagt
     2521 gatccccggc tatgagggct tgcgcgcttt gacccaggct cacgaaccgt tgctcaaagc
     2581 cgaatgcggc gccccgtggc aatcctattt tctgcggagc cactggcgga tggtgttccc
     2641 gatctggaag cggcaccagg agcggatcgc acaacaactg cggcagcgcg tgagccaagg
     2701 ctccgcgcca actgtgcaca tctttcggtt tcctcgcatc acgatcaacc acgggctcat
     2761 gctgtttgga atggtggagt cggagcgaga catccagttt gaagcctatg atccgaatct
     2821 cacggaccac ccggccaaac tcatttatga ccgggtccga aggagctttt catttccacc
     2881 cacctgctat tggcccgggg gagagctgag catcatcgaa atctatcgcg gcggactcta
     2941 ctgagccgaa ggagcacaaa cggagtgatg gagtgttggg agacaactgg ttacatgagt
     3001 tacatgatcc gccgccaacg cgccagcgtc cccgagcttg catgtcaggc aggcagcgct
     3061 taagctagcc ctatgagcat ggcggaactg atttactcga aggccaagac gctgcctgat
     3121 tcggcgcagg tcgcgttgct gcaactcgtg gagctgctcg ccaaagaccc gtccaccaag
     3181 ccgctggccc gcatcgagcc tggcagcgcc cgagggctgg tcagcatggc ggaggacttc
     3241 gacgcgccgt tggtggattt tgagccgtac atgaaatgaa cctgctgctg gacacgcacg
     3301 cgttcctttg gttcatcgcc agtgatcccc gcctcagcaa tcaggcgcaa tcgttgattc
     3361 agacgcccgc aaaccgccgc ctgctcagca tggcgagttt gtgggagatg gcgatcaagg
     3421 tcagcttggg caaactcacg ctcgctcaac ccttcgacca gttcatccct cggcagcttc
     3481 aattgaacca gatcgaagtg ctcgccatcg agttgtcgca tctggcggcg gtcgtggcga
     3541 tgccctttca tcatcgggat ccctttgacc gtctgatggc cgcccaatgc cagattgagg
     3601 gccttcccat tgttagcaac gatccggtct tcgacgctta ttccgttcgc cgggtttggt
     3661 agagttggcg ttgtcgccaa tcgctgcttg cagccatccg cgtcccactc cattattcca
     3721 ccactccatc ccttcctgag caccggttct tcgggtgcca gaagccgatg ccaaaaaaca
     3781 acgtgccgca
//
LOCUS       DATCGS010000300         4579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_571076, whole genome shotgun sequence.
ACCESSION   DATCGS010000300 DATCGS010000000
VERSION     DATCGS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571076"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1877)
                     /locus_tag="VIL39_06115"
     CDS             complement(<1..1877)
                     /locus_tag="VIL39_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY5481037.1"
                     /translation="MNMDWTHLLRNWLWQDRPQTGWTPDHLLHPKRFEAAPRMAALAL
                     LACVLFSGPIAFAGEQPLAKLRPVPFTDVKIQDSFWAPRRETNRLASIPFSLQKLEEA
                     GNLEDMRLAGHGATNGFRGPVFMDSDLYKALEAASYSLATHPDPALEKQLDDIISLMA
                     AAQQPDGYLNSYFTIKEPGKRWTNLRDWHELYCAGHMFEAAAAHYQATGKKTFLNIAT
                     RYADYIDSVFGPAPKRLGYPGHPEIELALIKLWRATSNQRYFELARFFVENRGRKFFA
                     TEHQTPLDKYDGSYWQDDVPIYDHQNIKGHAVRAAYLMSGVTEVASQTGDERLLKMLD
                     RVWRNTTERNEYITGGIGPSAHNEGFTVDYDLPNQSAYQETCATIALAQWAHRLALLY
                     GDAQYADVLERALYNGVLSGVSQDGTKFFYVNPLESAGTHHRSPWFGCACCPPNVTRT
                     LAALGGYAYAASADSLYVNLYIQGAAQAKVGDTAVALKVTTEYPWDGKVALTLTPASH
                     AKFALRLRVPGWCHNASVAVNRRAVKVAIVERGYLVLDREWNRGDQVELDLPMPVQRV
                     AADPHVKADQGLLAIQRGPIVYCLEQCDQAEPLASLWLPVEAELKAAREPRLLGGVVT
                     IT"
     gene            2089..3372
                     /locus_tag="VIL39_06120"
     CDS             2089..3372
                     /locus_tag="VIL39_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY5481038.1"
                     /translation="MEELIGNLLVAQSGGPTSVINASLAGVIQEAGRHDCIEQIYGGA
                     NGILGILNEELIDINEEKARTIDGLKYTPAAALGTCRYKIDFKKKPEKAAKDMDRLFQ
                     VFEAHNIRYFAYIGGNDSQDTSHKIHEEAVKRAYVMRVIGVPKTIDNDLPHTDNCPGY
                     GSVIKYNATTVTEVGLDVSSMATDDGSCCIIEVMGRSAGWIAAGTVLAKRGNPANAPH
                     IILLPELPFEEGKFLAKVKETVAAYKYCIVVVGEGIKDSSGHEVGADKTLLDSFGHPV
                     LAGAAEELKELVQGRLKTKTRTVLLGYAQRAAAHCASLTDATNGFACGEAAVKAAIAG
                     QSGLMVKIVRDTQPDGSVKWSTGLQSLADIANVEHFIPREWISEDGYLPNEKFVTYAQ
                     PLIEGEVKPPVEGGLPKYVVLEKSKVEKKLPARET"
     gene            3942..4481
                     /locus_tag="VIL39_06125"
     CDS             3942..4481
                     /locus_tag="VIL39_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481039.1"
                     /translation="MKTKDILTIATVALGTATLTVAAFWAGPIEAGSDADAPPAKLAK
                     ARLVSHGVELTLASAGGRLFKAGDQPEFELTALNTTNQPVSVAVCVSMTAASPADALS
                     RVIRLPTVLWQQEQVVTLQPNETKVLALCASTNLPPNSVISVSLREQDQKAAPFSPGI
                     VALSFSTVVPKALPTVALQ"
BASE COUNT          888 a         1380 c         1433 g          878 t
ORIGIN      
        1 gttatggtga ccacgccgcc gagcagcctg ggctcacgcg ccgccttgag ttcagcttcc
       61 accggcagcc aaagcgacgc gagcggctca gcctggtcgc actgctccag gcaatacaca
      121 atcggcccgc gctgaatcgc cagcagtccc tgatcggctt tcacatgcgg atcggcggca
      181 acccgctgga ctggcatcgg gaggtccagt tccacttggt cgccccggtt ccattcgcgg
      241 tcgaggacca ggtaaccccg ctcgacgatc gccaccttca ctgcacgacg gttcaccgcg
      301 acgctggcgt tgtggcacca gcccggcacc cggagccgca gggcaaactt ggcgtgcgat
      361 gccggggtga gcgtcagcgc cactttgccg tcccaagggt actcggtcgt caccttgagc
      421 gctacggcgg tgtcgccgac cttcgcctgg gcggccccct ggatgtagag gttgacgtag
      481 agcgaatcgg cgctggcggc gtaagcataa ccgcccagcg cggcaagggt acgcgtgaca
      541 ttcggcggac agcaggcgca gccgaaccac ggcgagcggt ggtgcgtgcc ggcgctttcc
      601 agggggttga cgtagaagaa cttcgtgccg tcctgcgaga cccccgagag cacgccgttg
      661 tagagcgcgc gctccagcac gtcggcgtat tgcgcatcgc cgtagagcag cgccagacga
      721 tgcgcccact gcgccagcgc gatggtcgcg cacgtttcct gatacgcgct ctggttcggc
      781 aaatcatagt ccacggtgaa cccttcgtta tgggcgctgg gtccaatgcc gcccgtaata
      841 tattcattgc gctcggtggt gttgcgccag acacggtcca gcatcttgag caggcgctcg
      901 tcccctgtct gcgaggccac ttcggtgaca ccggacatca ggtaggctgc tcggacggcg
      961 tggcctttga tgttctgatg gtcgtagatc ggcacatcgt cctgccagta agagccgtcg
     1021 tatttgtcca gtggcgtctg gtgttcggtg gcaaagaact tccggccgcg gttctcgacg
     1081 aagaagcggg ccagctcgaa atagcgctgg ttgctggtgg cgcgccagag tttgatcagg
     1141 gccagttcaa tctcgggatg tcccggatag cccagccgct tgggcgcggg gccaaacacc
     1201 gaatcaatat aatccgcata ccgggtggcg atgttcaaaa aggtcttctt gccggtggcc
     1261 tggtagtgcg cggcagccgc ctcaaacatg tggccggcgc agtagagctc gtgccagtcg
     1321 cgcaggttgg tccagcgctt gccgggttcc ttgatggtga agtagctgtt gaggtagccg
     1381 tcgggctgct gggctgcggc catcaggctg ataatatcgt ccagttgctt ctccagcgct
     1441 ggatcggggt gggtggcgag cgagtatgag gcggcttcga gcgccttgta gaggtctgag
     1501 tccatgaaga ccggcccgcg gaagccgttg gtcgcgccat ggccggctag ccgcatatcc
     1561 tctaggttgc ccgcttcctc gagcttctga aggctgaaag ggatgctggc caagcggttc
     1621 gtctcgcgcc gcggcgccca gaaggaatcc tggatcttaa cgtccgtgaa cggcacaggc
     1681 cgcagcttcg ccaagggctg ctccccggca aatgcaatag gcccggagaa caacacgcag
     1741 gccaggagag caagtgccgc catccgtggc gcggcttcaa acctctttgg gtggaggaga
     1801 tggtctgggg tccagccggt ctgcgggcga tcctgccaca gccaatttct caacagatgc
     1861 gtccaatcca tgttcataag atttctcaag tgttgagcac atggttgggt tttgcgctgg
     1921 actgtcaatc ccgttcgggc agccgcgata caggggtagg cgatttcttt gcgatttccc
     1981 ctttgacaga ggcccgtttc attggcatgg ttccgctccg ttttggatgg gcttccaaac
     2041 atggagagct gataggcttc agctcgacgc ggaagtaatt ggaatattat ggaagaactt
     2101 attggaaatc tcttggtggc gcagtcgggc ggtcctacgt ccgttatcaa cgccagtttg
     2161 gccggtgtga ttcaggaagc gggccggcac gattgtattg aacaaatcta cggcggcgcc
     2221 aacggcatcc tgggcatcct gaacgaggaa ctcattgaca tcaatgagga gaaggcccgg
     2281 actatcgacg gcctgaaata cacgcccgct gccgcgctcg gcacctgccg gtataagatc
     2341 gatttcaaga agaagcccga gaaggcggcc aaagacatgg accggctgtt ccaggtgttt
     2401 gaggcccata acatccgtta cttcgcttac atcggtggca acgactcgca ggacacctcg
     2461 cataagatcc acgaagaggc cgtcaaacgg gcctatgtta tgcgcgtcat cggtgtgccc
     2521 aagaccattg acaacgacct gccgcatacg gacaactgcc ccggctacgg atcggtcatc
     2581 aagtacaacg ccaccacggt gacggaagtc ggcttggacg tgagcagcat ggccaccgac
     2641 gacggctcct gttgcatcat tgaagtcatg ggccgctcgg cgggctggat cgcggccggc
     2701 acggttctgg ccaagcgggg caacccggcg aacgcgccgc acatcattct attgcctgag
     2761 ctgccgttcg aagaagggaa gttcctggcg aaggtgaagg agaccgtcgc ggcttacaaa
     2821 tactgcattg tggtcgtggg cgaaggcatt aaggactcct cgggccatga agtcggcgcg
     2881 gacaagaccc tgctggactc gtttggccac ccggtgctgg cgggggcggc ggaagaactt
     2941 aaagaactcg tgcagggccg gctcaaaacc aaaacccgca ccgtgctgct tggctatgct
     3001 cagcgtgccg cggcgcattg cgcgagtctt actgatgcca cgaacggctt cgcctgcggc
     3061 gaggctgccg ttaaggctgc catcgcgggc caaagcgggc tgatggtcaa gatcgtccgt
     3121 gacacgcaac ccgatggctc ggtcaagtgg agtaccggtt tgcagtcgct ggctgacatt
     3181 gcaaacgtcg aacactttat tccgcgcgag tggatcagcg aagacggcta cctgccgaac
     3241 gagaagttcg tcacctacgc ccagccgttg atcgaagggg aagtcaagcc cccggtagaa
     3301 ggcggcctgc ccaaatacgt cgtgctcgaa aagagcaaag tcgagaagaa gctcccggca
     3361 cgcgaaacct gaaccgccag ttatctggtg gccaagtcat ccgcggtcca catgacttta
     3421 tctggaccag tggagtaaag ctccatttcc gctcctgaga gccgatggaa gaatagcgga
     3481 gtacccccag taatccacca gttcgcctgt gccgctgatg cgcaggcctc actcctcctc
     3541 gaagcaggca ccggtgccgc aggggcactg aagttcccgc ggatgccctc cggatgcctt
     3601 ccggggtgcc acgctctctc cgcctcgtag tgtcaaaggc gaccttcgga tatccctggc
     3661 aagctctctt gtactcgggc cgtttcatct tcgatccgcc atcctccagt tgcccgggct
     3721 gccgcttggc cacgccaccg tcctgccgtg cgggcgcgga agtccgcgcc aacgagcggc
     3781 gcgatggtgg cgccggcggg agcagattct tttacacact ctttacgtct gtttgacaac
     3841 tcgttcggcg gcctgggctt tattggatgc gaaagttgga ttgcccggcg ctgggccggg
     3901 agatcgagaa acaatgacac gagcgaaaca ggaaacagac aatgaaaacg aaagatatac
     3961 tgactattgc aacggtggcc ctcggcaccg caacgttaac ggtggcggcc ttctgggccg
     4021 gccccatcga ggctggcagt gacgccgacg cgccgcccgc caaactcgcc aaagcccggt
     4081 tagtctctca cggggtcgag ctgacgctcg cctccgctgg cggccggctc ttcaaggcag
     4141 gcgatcagcc ggagtttgaa ctcacggcgc tcaacaccac caatcaaccg gtcagcgtcg
     4201 cggtctgcgt cagcatgacc gctgcgtcac cggcagacgc gctgtcccgc gtgatccgcc
     4261 tgccgacggt tctgtggcag caggagcaag tcgtgacgct ccaacccaat gagacgaaag
     4321 tgctcgcgct gtgcgccagc acgaacctgc cgccgaacag tgtgatttcc gtctcgctcc
     4381 gtgagcagga ccagaaagcc gcgcccttct cgccaggcat cgtggcgctg agtttctcca
     4441 ccgtggttcc caaggcactg ccgacggtcg cactgcaata gtcgaaattg ctcacaggct
     4501 acgaagatgt ttctgggtga acggccacgc gaagcgtatg gagtgcgacc agcttgctgg
     4561 cgctttcgag cggtgccgc
//
LOCUS       DATCGS010000301         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_472738, whole genome shotgun sequence.
ACCESSION   DATCGS010000301 DATCGS010000000
VERSION     DATCGS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472738"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VIL39_06130"
     CDS             <1..412
                     /locus_tag="VIL39_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5481040.1"
                     /translation="WAGSLNQGQLTPQQRELLALTIAEINGARYCLSAHYALAKQLGL
                     DDNEIRAARRATSADPQTHAMLRFAQSVALQRGELSDADFQSVRQAGFTDALITEIIS
                     NIALNIFTNYFNSIARTDNDFPLLKPGADTPARA"
     gene            481..2037
                     /locus_tag="VIL39_06135"
     CDS             481..2037
                     /locus_tag="VIL39_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY5481041.1"
                     /translation="MRIGWILLMISALGLNAADDAVPAPAAGKTNGPVAVKKDESKKE
                     EEKKSAATIPDAAHKPVLITNTVTIAGHAVTYVAETAMLPLLKPDGTVRASIFYVAYT
                     RQGETNVATRPVTFCFNGGPGSSSVWLHLGALGPRRVRMNENGTLPKPPFSLLDNEYS
                     LLGATDLVFIDPVATGFSRPAKDEKADQFFGQSGDIESVGDFIRLWTTRHQRWLSPKY
                     LCGESYGVFRAAGLAGYLASRYGMYLNGLILVSGVLDFETLHEGRGNDLAALVFLPAY
                     TATAHFHQKLPPDLQSDLNKALAEARDFIKTEYLSALLQGAALPAAERARVVAKLARL
                     TGLPPRVIEDNDLRVSSGTFRRMLLHDQGLVLGRYDGRITGRDADPAAPAPRFDPSFT
                     ATYGPFSAAMNAYVRGELNFEDDLPYEILAGVGPWNYDSRNSYPSVTGQLAAVMRENP
                     YLRVLVLGGLRDLACPMDGIRNSLDHLQLDSAYRGNITFAEFDSGHMMYVNLPDLKKM
                     QGELEKFVRQ"
     gene            complement(2227..>3051)
                     /locus_tag="VIL39_06140"
     CDS             complement(2227..>3051)
                     /locus_tag="VIL39_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481042.1"
                     /translation="TKDLPVAVSSLANLRDSPGKLGEMSAYLDFGNMHPYAAGYPPSQ
                     HWGWGLSMERAIAEAQKVSGNKPILVTECGYHNRVENQGHPGVTEVAEAKYLPRLLFV
                     YFNRGMTKAYKYELLDLKPDPGITDLERHFGLVRTNGTPKPSFYALKNLLHLLADSGS
                     ATPAGSLAFQLSGATNNLHHTLLQKRDGTFWLALFQETVSYDPKTRGDLEVPSQPVTL
                     KLPWTSPGVWLYRPNRSSEPTEHIAGSSEATLQVPDEVLLVEIKPPKHERGSPGKE"
BASE COUNT          580 a          913 c          964 g          594 t
ORIGIN      
        1 ctgggccggg tcgctcaatc agggccagct cacgcctcaa cagcgcgagc tcctggcttt
       61 gaccattgcc gaaatcaatg gcgcccgata ctgcctctcc gcccactacg cgttggccaa
      121 gcagttgggc ctcgacgaca atgagattcg tgctgctcgc agagcgacct ccgcggaccc
      181 ccagacccac gccatgctgc gctttgctca atccgtcgcc ctccagcgcg gcgagctgag
      241 cgatgcggac tttcaatccg tccgccaggc gggcttcacc gatgccctga tcacggaaat
      301 catctccaac atcgccctga acattttcac caactacttc aactccatcg cccgcacgga
      361 taacgacttc cccttgctca agcccggcgc cgatactccg gcgagagcgt gagccaatcc
      421 ttcccttcgc tgccaaagac cgggctggcg gcgaacaggg atatggttca ggatcggcgc
      481 atgagaattg gctggatact actgatgatc tcggcgctag ggttaaacgc ggcagacgac
      541 gcggtgccgg cgccggcggc cgggaagaca aatggtcccg tggcggtgaa gaaggacgag
      601 agcaagaagg aggaggagaa gaagtccgcc gcgaccattc cggatgcggc gcacaagccg
      661 gtgctcatca cgaacaccgt caccatcgca ggccacgccg tgacctacgt cgcggaaacc
      721 gcgatgttgc cgctgctgaa acccgacggc accgtccggg cttcgatatt ctacgtcgcc
      781 tacacgaggc agggcgaaac caacgtggcg acgcgaccgg tgaccttttg tttcaatggg
      841 gggccgggct cttcctccgt gtggctgcac ctcggggcct tgggaccgag gcgggtcagg
      901 atgaacgaga atggcacgct gccgaagccg ccgttcagcc ttctggacaa tgagtattcg
      961 ctgctgggcg ccacggacct ggtgttcatt gatccggtgg cgacgggttt cagccggcca
     1021 gcgaaggatg agaaggcgga ccagttcttc gggcaatccg gcgatattga atcggtgggg
     1081 gacttcattc ggctttggac gacgcgccat cagcggtggc tgtcgcccaa gtatctgtgc
     1141 ggcgagagct acggggtgtt tcgggcagcc gggctggcgg gttacctcgc ctcgcgctac
     1201 ggcatgtatc tcaacgggct gatcctggtg tcaggggtgc tggacttcga gacgctccac
     1261 gaaggacgcg ggaatgatct ggcggcgctg gtctttctgc ccgcctacac cgcgacggcg
     1321 cactttcacc agaagctgcc gcccgacctg cagtcggacc tgaacaaggc gctagccgag
     1381 gcgcgcgatt ttatcaagac ggagtatctg tcggcgttgc tgcaaggggc cgcgctgccg
     1441 gcggcagaac gagcgcgggt ggtggcgaag ctggcgcggc tgaccgggtt gccgccgcgc
     1501 gtgatcgaag acaatgacct gcgggtcagc agcgggacgt ttcgccgaat gctcctgcac
     1561 gaccaggggt tggtgcttgg ccggtatgac gggcgcatca cgggccggga cgcggacccg
     1621 gcggcaccgg cgccccgatt cgacccttcc ttcacggcta cctacggccc gttctcggcg
     1681 gcgatgaacg cttatgtgcg gggcgagctg aatttcgagg acgatctgcc gtatgagatt
     1741 ctggccgggg tcggaccgtg gaattacgac agccgaaaca gctatccgag tgtgacgggc
     1801 cagcttgccg cagtgatgcg ggagaacccc tatttgcggg tgctggtgct gggtggcctg
     1861 cgcgacctgg cgtgcccgat ggatggcatt cgcaacagcc tggaccatct gcaactcgat
     1921 tcggcttacc gcggcaacat caccttcgcc gagttcgatt ccgggcacat gatgtacgtc
     1981 aacctgccgg acttgaagaa gatgcagggg gagttggaga agttcgtccg gcaataagtc
     2041 cgccgtttcc agaatgggag cgcctcgcgc tcaacaggag ctgaacgacg cgcgagcgca
     2101 caacctgaat ttgccggaaa cgacgtcacg ccaggcggtc atggaactac tgacctggct
     2161 tgcaataagg tgtaggtggc ggggttatcc cctcctgcac ccaacccaac cacctgtcaa
     2221 ccctgattac tcttttccgg gactgcccct ttcatgtttg ggaggtttga tctcgaccaa
     2281 caatacctcg tctgggactt gcagcgttgc ttcacttgac cccgcaatat gttctgttgg
     2341 ctcagaagat ctgttgggcc gatacagcca aacccctggc gaagtccagg gcagcttcaa
     2401 ggtgacgggt tgtgagggca cttccaggtc gccgcgcgtc ttgggatcgt aagacaccgt
     2461 ttcttgaaag agcgccagcc agaaagtgcc atctcgcttt tggagcagcg tgtggtgcaa
     2521 gttgttcgtc gcgccggaaa gctgaaaagc cagtgagccc gctggagtcg cggacccgga
     2581 atcagccagc aggtgcagga ggttctttag ggcgtaaaag gacggcttgg gcgtgccgtt
     2641 cgtgcggacc aggccaaagt gcctctccag gtcggtgata ccggggtctg gtttgaggtc
     2701 gagcaactca tacttgtagg ccttggtcat gccccggttg aaatagacga ataacaggcg
     2761 tggcaggtat ttcgcctccg ccacttccgt cacgccggga tggccctggt tctcaactcg
     2821 attgtggtaa ccgcattccg tcaccagaat cggcttgttg ccgctaacct tttgggcctc
     2881 ggcaatggcg cgctccattg agagccccca accccaatgc tggctgggtg gatatccggc
     2941 tgcgtaaggg tgcatattcc caaaatccag gtatgccgac atctctccca gcttgcctgg
     3001 gctgtcgcgc aagttcgcca gcgatgagac tgccaccggg agatctttgg t
//
LOCUS       DATCGS010000302         9241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_372014, whole genome shotgun sequence.
ACCESSION   DATCGS010000302 DATCGS010000000
VERSION     DATCGS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372014"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..454
                     /locus_tag="VIL39_06145"
     tRNA            379..454
                     /locus_tag="VIL39_06145"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:412..414,aa:Phe,seq:gaa)
     gene            complement(577..2346)
                     /locus_tag="VIL39_06150"
     CDS             complement(577..2346)
                     /locus_tag="VIL39_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481043.1"
                     /translation="MKNSLPLFSTLAGFLGLLLGITVSGQTPEAINPGVPWPATDALG
                     RPLPLADAVGPVRTDRFVGIFYFLTHTGGDQRTEPYDVSKILAQDPQAVKKPDSPLWG
                     PLHTSHYWGEPLYGYYRSADPWVLRRHAQLLADAGIDTLIFDTTNAQTYRDVYMALCE
                     VFRQVRQSGGRTPQIAFMVNTKAGETALKIYKDLYQPGLYPELWFRWQGKPLLICDPQ
                     EASAELRAFFTLRRAHWPFTLTNTPFAWHWEATYPQPYGYTEDQTKPEQVNVSVAQNL
                     RAKDGKVTNMSFGDARGRSFHDGREDRSPGAVNFGYNCQEQWKRAFALKPPFVMVTGW
                     NEWTAGRWGEAGGPIVFVDQFDQEFSRDIEPVKGGHGDNYYWQLIANVRRFKGAPPLP
                     KASASKTIRVADGFEQWRSVGPEFLDHVGETAARDFGGAAGLHYTNRTGRNDLVALKV
                     ARDEKNVYFYARTREPISPSSDSNWMWLLIDSDQDCKTGWAGYDFIVNRTVEADGKTW
                     LEQNQGGWEWKRIQALNFRVSGNELQLAIPRAALGLGKGTTRFALDFKWADNLQKPGD
                     VMDFFISGDVAPEGWFNYRYVAE"
     gene            complement(2393..4543)
                     /locus_tag="VIL39_06155"
     CDS             complement(2393..4543)
                     /locus_tag="VIL39_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY5481044.1"
                     /translation="MSEANKHFSVTRAASMNRRGASSHFLSGRFVTVLTMCLLALASQ
                     DLFAARLHVATDGRDTNPGTKAKPFATLERARDEVRRLKASGSLPSDGITVEVRAGVY
                     ELSRPLELTDQDSGREGAPVVYQARKGDSVRLMGGRVVTGWTPVTDPAVRARLEEKAR
                     DQVRQADLRTLGIKDLGDVVARGKRLELFFQDQPMTVARWPNSGFITITDVRGPTEVD
                     IRGTKGRKEGLFTYDGDRPNRWGAEPDIWVHGYWFWDWAEQRHPVASVDTAKHLITVK
                     PPYHSYGYRKGQWFYAFNLLAELDRPGEWYLDRRTGILYFWPPAPLEQGQAVVSVIPT
                     LVQMRNASYVTVRGLVLEACRATAIEMSGGRGNLIAGCTIRNTGQEAVNVTGQDSGVV
                     GCDIAETSGGGIRLNGGDRASLTPAKLYADNNHIHHFSRWDRVYQPGISLGGVGNRAT
                     HNLIDNAPHMAIGFSGNDHLIEFNEIHSVCYESNDAGAIYAGRDWTMRGTRIRNNYFH
                     HISGFAQRGCVGVYLDDQFSGTEITGNVFYKVTRAAMIGGGRDCTIANNIFVDCVPAT
                     HVDARGLGWAAGGFEGLKDSLNALPTKRPPWSTRYPKLVTLLDEQPMSPVGNVLTRNI
                     CVRGRWGDFESKAKPLITFRDNLLDQDPLFVDAARQNFQLRDDSPAFKLGFERIPLER
                     IGLYRDKLRASWPVTHFVRPDTADSASAPAPTGT"
     gene            4661..7489
                     /locus_tag="VIL39_06160"
     CDS             4661..7489
                     /locus_tag="VIL39_06160"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY5481045.1"
                     /translation="MEQHAIRLRVAGRFTGAGVLSALALVLALAVPLAKGAAASGEAT
                     GRPGHQAQTLQEMFRNPPADSRILKIIHSWPDGEAEQDALIHSLAAKGFGGVVCNVSF
                     AEYLASEAKWKAFARAVQAAKQAGFALWLYDEKGYPSGTAGGIVLREHPEWEARGLLV
                     ADAEGDAGTITVDVPPGKPFLMAAFPVQEGQIVLRGMTNLAAQVHEGRLSWSPPPGRW
                     KMLAITESRLFEGTHASMSLSDHIPYLNLLQPEPTARFLAATHEAYAQHLGPDLGRWF
                     VSTFTDEPSLMSLFLRQMPYRVLPWSPNLPVEFKKRRGYALEPQVPALVADAGPAGQR
                     ARYDYWQTVGELVAESYFGQIQAWCARHHLLSGGHLLMEEDLVNQVPLYGDFFGCLRR
                     LDAPSIDCLTSIPGQVPCFIARLAGSAADLENRTVTMCETSDHSQRYRPAGDQRPVVH
                     VTEEQIRGTCNRLFVGGISTITSYYSFAGLSDEQLRHLNEWVGRCCAALKGGHRVADI
                     AVLYPIQSVWPRFNPAHRLASESPAAARIETLYREVSDGLFAAGRDFAYVDGRALAEA
                     KVAGGALVHGKLRWRVVVLPGADTLPLAAWENLARFVRSGGVLISVGDLPANSEAEFP
                     SPRVQALVRDLFGPGEGGPRVSAKRHGGGAIFLPGSSAALLPTVISQVLAPDVEVAGT
                     HSPIRPTHRRINGQEVYFLINDSAQPWSGRVGLSVDGPGEYGDPGTGQVTPLASGSDV
                     PLSLPSYGAALFRFPKTRPPKLLGFGKNVLPQLATSDLPEASPKVACGEFVREQLTPE
                     AAPRGEGRRAWQAVGRLTKSQVDTFLFVRFPYADGLDLRKAEWLVLDTWVPPAQRTPT
                     QLLVVLRQKNGAEYLASTGRYLGAAGYEQTCLPLSRFQLAGWSNDPNGRLDPSSVTEI
                     RVGWGGYFGSEGEKVEFTLCAPRSATTQ"
     gene            complement(7543..9111)
                     /locus_tag="VIL39_06165"
     CDS             complement(7543..9111)
                     /locus_tag="VIL39_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEY5481046.1"
                     /translation="MSNMNGKRLLASILLTGTLTAQLAGGAENSTATASKLVIHADQA
                     KDTISRNIYGHFSEHLGRCIYEGIWVGEDSSIANTRGIRNDVVAALKKIKVPVLRWPG
                     GCFADEYHWKDGIGPRSQRPTMINTHWGGVVENNHFGTHEFLDLCEQLGCEPYLCGNV
                     GSGSVQEMQQWVEYITFDGVSPMAELRKQNGHPEPWKLKFFAIGNENWGCGGNMTPQY
                     YADQYKRYATYVRNFGGNQVYKVACGPNGANYQWTETLMREAGKRMAGLALHYYCGSG
                     KKTQLATEFDEGDWFAQLQRALRMEELVTKHSEIMDKYDPDQKVALIVDEWGTWHLVE
                     PGTNPGFLYQQNTLRDALVAGVTLNIFNNHCRRVKMSNIAQMINVLQAMILTDKEKMI
                     LTPTYHVFEMYTVHHDAKLLPSEVQSADYQSGSDKLPQVNVSASRDQAGRIHVTLCNL
                     DPKNAAEVACDLQGAKATKVSGRILTAPAMNAHNTFEEPETLKTAEFAGARIASGGFA
                     ATLPAKSVVVLELE"
BASE COUNT         1669 a         2833 c         2904 g         1835 t
ORIGIN      
        1 gcgccttcag cgctggagac cgcctttagg gcaatgaacc cagggcgttt gccctgggct
       61 ggtatgaatg acgccttcgg cgtctcaacc gcgtccgcag agcggcctcg ggtctgcatg
      121 gaagaattct gtcctgcgcc cgtccgtttt tcagcacttg caattcggca gcggtttgtg
      181 gcatagtgcc ttcgtgttcg agacgaacgc aagttttgtg actgccgacg cagatctcag
      241 cgcagtagat attgttgcgc agcgagagtc cgcgcacgcg tttcccgtcg tggtcgcaaa
      301 ctggagggaa cttgttgaac acaggggcag gcacggcctt tttgctgcag atgcttggtc
      361 tcgtgcatgc cacaggttgg ctgggtagct cagttggtag agcagaggac tgaaaatcct
      421 tgtgtcggcg gttcaattcc gcccccagcc accacttcaa aacccaataa aatcaagctt
      481 tctgtgagtt ttccaagccc ttcaccctaa aagaccactc tgcgcttgga ctaactcggc
      541 aactgttcct gaaaccatgg acgccgtccg gaaaagctac tcagccacat accggtaatt
      601 gaaccagcct tcgggagcca cgtcgccgct gatgaagaaa tccataacat cgccgggctt
      661 ctgcaagttg tccgcccatt tgaaatcgag ggcgaaccgg gttgtccctt tccccaagcc
      721 gagcgccgcg cgtgggatgg ccaattgcag ctcgttgcca ctgacacgga agttgagggc
      781 ctgaattctt ttccattccc agccgccctg attctgttca agccaggttt tgccatcggc
      841 ttcaacggtg cggttgacaa tgaaatcata gccggcccag ccggttttac aatcctggtc
      901 cgaatcaatc aaaagccaca tccaattgga atcgctggag ggcgaaatgg gctcgcgcgt
      961 tcgggcgtag aagtaaacgt tcttttcatc gcgggcgact ttgagggcaa ccaggtcgtt
     1021 gcggccggtg cgattagtgt aatggagacc agcagcgccg ccgaaatcgc gcgcggctgt
     1081 ctcgccgacg tggtccagga attccggacc aacagagcgc cattgctcaa agccgtcggc
     1141 gacacggatg gtctttgagg ctgaagcctt tggcagtggc ggggcgccct tgaaccggcg
     1201 gacgttggcg atgagctgcc aatagtagtt gtcgccatga ccccctttca ccggctcgat
     1261 gtcacggctg aattcctggt cgaattggtc cacgaaaaca atggggccgc ccgcttcgcc
     1321 ccagcggccg gcggtccatt cgttccaacc ggtgaccatc acgaacgggg gcttgagcgc
     1381 aaaggcgcgc ttccattgtt cctggcaatt gtaaccgaag ttgaccgcgc ctgggctgcg
     1441 gtcctctctg ccgtcatgaa agctgcggcc ccgagcgtcg ccgaaactca tgttggtcac
     1501 cttgccatcc ttggcccgca agttctgggc aacggaaaca ttgacctgct cgggcttggt
     1561 ctgatcttcc gtatagccat aaggctgggg ataggtcgct tcccaatgcc aggcaaacgg
     1621 ggtgttggtg agagtaaacg gccagtgagc gcggcgcagg gtaaagaacg cgcgcaactc
     1681 agcgctggcc tcttgcggat cacagatcag caacggcttg ccctgccagc ggaaccatag
     1741 ctcgggataa aggcccggtt gataaagatc cttgtatatc ttcagcgccg tctcaccggc
     1801 tttggtgttc accatgaagg caatttgcgg ggtgcgcccg cccgactgcc ggacctggcg
     1861 aaagacttcg cacagcgcca tatagacatc gcggtaagtc tgagcgttag tagtatcaaa
     1921 aatcagggtg tcaatgccgg cgtcggccag caactgggca tgccggcgca gcacccaagg
     1981 gtcggcactg cggtagtagc catagagcgg ctcgccccaa taatggctgg tatgaagcgg
     2041 tccccaaagg ggcgaatccg gcttcttaac cgcttgagga tcctgggcca gtatcttcga
     2101 gacgtcgtaa ggctccgttc tctggtcgcc gcccgtatga gtaagaaagt agaagatgcc
     2161 gacgaagcgg tcggttctca ccgggccgac tgcgtcggcc agcggcaaag ggcgacccag
     2221 cgcgtccgtg gccggccagg gcacgccggg gttgatggcc tccggggttt gcccggagac
     2281 ggtaatgccc agcaggagac ctagaaatcc cgctagcgtt gaaaacaggg gcagggagtt
     2341 tttcataagt tttggatcag ccgtcatgtc gcacaacgcg cacagtattg gctcacgtcc
     2401 cagttggtgc cggggccgat gcggaatcgg ctgtgtcggg ccggacaaag tgcgtgaccg
     2461 gccacgaggc ccgcaactta tcccgataga gaccgatccg ctcgagggga atccgctcga
     2521 agcccagctt gaaggcgggc gaatcgtcgc gaagctggaa gttctggcgc gccgcgtcca
     2581 cgaagagcgg gtcctggtcc agcaggttgt cgcggaaagt gatcaagggc ttcgccttgc
     2641 tctcgaagtc gccccaccga ccgcggacgc agatgttgcg cgtcaggacg ttccccaccg
     2701 gcgacatcgg ctgctcgtcg aggagggtca cgagcttcgg ataccgcgtg ctccacggcg
     2761 gccgtttggt gggcagcgcg ttgagcgagt ccttcagtcc ctcgaagccc ccggcggccc
     2821 agcccagccc gcgcgcgtcc acatgcgtcg cgggcacaca gtccacgaag atgttgttcg
     2881 cgatcgtgca gtcgcggccg ccgccgatca tcgccgcgcg cgtaaccttg tagaacacgt
     2941 tgccggtgat ctctgtgccg ctgaactgat cgtcgagata gacgcccacg cagccgcgtt
     3001 gcgcgaaccc gctgatatgg tggaagtaat tgttccggat gcgagtgccg cgcatcgtcc
     3061 agtcgcggcc cgcgtagatc gccccggcat cgttggactc gtagcacacg ctgtggatct
     3121 cgttgaactc gatgagatga tcgttgccgc tgaatccaat cgccatgtgc ggcgcgttgt
     3181 ctatcaggtt atgcgtcgcc cggtttccca cgccgcccag gctgatgccc ggttgataga
     3241 cacggtccca acggctgaag tgatggatgt ggttgttgtc cgcgtatagc ttcgccgggg
     3301 tgaggctcgc gcggtcgccg ccgttgagcc ggatgccgcc gccggacgtc tcagcgatgt
     3361 cgcacccgac cacgccgctg tcctgacccg tcacgttgac ggcctcctgg cccgtgttcc
     3421 ggatggtgca tccggcgatc agattgcctc gaccgccgga catctcaatc gccgtggccc
     3481 gacaggcttc aaggaccagg ccgcggaccg tcacatacga cgcattccgc atctgaacca
     3541 gcgtcgggat gaccgagacg acggcttgcc cctgttccag gggggccggc ggccagaagt
     3601 agaggatccc ggtgcggcgg tcgagatacc actcgccggg gcgatccagc tccgccagca
     3661 ggttgaacgc gtagaaccac tggcccttgc ggtagccgta ggagtggtaa ggcggcttga
     3721 cggtgataag atgcttggcg gtgtccaccg aggcgaccgg atggcgctgc tcggcccagt
     3781 cccagaacca gtagccatga acccagatgt cgggttcggc gccccagcga ttcgggcggt
     3841 cgccgtcgta ggtgaacagc ccctccttgc ggcccttggt tccgcggatg tccacctcgg
     3901 ttggcccgcg cacgtcggtg atcgtgatga acccggaatt cggccagcgg gccacggtca
     3961 tgggctggtc ctggaagaac agttcgagcc gcttgccgcg cgccaccacg tcgcccaaat
     4021 ctttgatccc gagcgtccga aggtcggctt gccggacctg gtcgcgcgcc ttttcctcca
     4081 gacgcgcccg cacggctgga tcggtgaccg gcgtccaccc ggtcaccacc cgcccgccca
     4141 tgagccggac gctgtctcct ttccgggcct gataaacgac gggcgcacct tcgcggccac
     4201 tgtcctgatc cgtcaattcc agcggtcggg acagctcgta aacgcccgcg cgcacctcga
     4261 ccgtgatgcc gtccgagggc agcgagccgg acgccttgag ccggcggacc tcgtcccggg
     4321 cacgctccag ggtggcaaat ggcttcgcct tggtgccggg attagtgtcc cggccgtcgg
     4381 tggctacgtg gagcctggct gcgaacaagt cctggctcgc gagcgccagg aggcacatgg
     4441 tgaggaccgt cacgaagcga cctgatagga aatggctgct tgcgccccgt cggttcattg
     4501 acgcggcgcg agtgactgag aaatgcttgt tggcttcact catcgcggga aggacagtgg
     4561 ccgaagcgcc cgtcgcggtc aagtgtcatt agaaacaccg ctcttcggat aatgtcctcg
     4621 acaattatcc gacagccgcc gttacttgga ctcgatgcta atggaacagc acgctatcag
     4681 gttgagagtt gccggcaggt ttacgggcgc cggcgttttg tccgcgctgg ccttggtcct
     4741 ggccctcgct gtgccgctgg caaagggtgc ggcagcgagc ggggaagcca cgggccggcc
     4801 gggccaccag gcacaaaccc tccaggagat gttccggaat ccgcccgccg attcgcgcat
     4861 tctgaagatc atccattcct ggccggatgg agaagcggaa caggacgcgt tgattcactc
     4921 gcttgcggcg aagggttttg gcggcgtggt ttgcaatgtg tccttcgccg agtatctggc
     4981 gagcgaggcg aagtggaaag cgttcgcccg ggcagtccag gcggccaagc aggccggttt
     5041 cgcgttgtgg ctatatgacg aaaagggtta tccctcggga acggcaggcg ggattgtctt
     5101 gcgcgagcac ccggagtggg aagcgcgcgg tttgctggtc gcggatgctg agggtgacgc
     5161 gggaacgatc acggtggatg tgccgccggg caagcctttc ctgatggctg ccttcccagt
     5221 gcaggagggc cagatcgttc tgagagggat gaccaacctg gcagcgcaag ttcacgaggg
     5281 caggttgtcc tggtccccac cgccaggccg ctggaagatg ctggccatca ccgagagccg
     5341 gctttttgag ggcacccacg cttcgatgag tctcagcgac cacattccct atctgaacct
     5401 cctccaaccg gagccgaccg cgcggttcct ggccgcgacg cacgaggcct acgcgcagca
     5461 cctggggccg gaccttgggc gctggtttgt ctccacgttc acggacgagc cgtcgctgat
     5521 gagcttgttc ctgcggcaga tgccgtaccg ggtcttgccc tggtcgccga acctgccggt
     5581 cgagttcaag aaacggcgcg gctatgcgct cgaaccccag gtcccggcgc tggtcgctga
     5641 tgccgggccg gctgggcagc gtgcgcgcta cgactattgg caaaccgtcg gcgagctggt
     5701 cgcggaaagc tacttcggcc aaatccaggc ttggtgcgcc cgccaccatc tgctctcagg
     5761 cggccatcta ttgatggagg aagacctcgt gaaccaagtg cccctgtatg gcgatttctt
     5821 cggatgcttg cggcggctgg atgcgccgag cattgattgc ctgacaagca tccccggaca
     5881 ggtgccgtgc ttcatcgcac ggctcgcggg cagcgcagcg gacctggaaa accggactgt
     5941 gaccatgtgc gagacgtcgg accatagcca gcggtatcgt ccggcgggcg accagcgccc
     6001 ggtggtccac gtcacggaag agcagattcg cggcacgtgc aaccgcctgt tcgttggcgg
     6061 catctccacg atcaccagct attactcctt cgccggcctt tccgatgagc aactgcgaca
     6121 tctgaacgag tgggttggcc gttgctgcgc cgcgctcaag ggcgggcatc gtgtggccga
     6181 catcgcagtg ctgtatccga tccagagtgt ctggcctcgg ttcaaccccg cgcaccgcct
     6241 tgccagcgag tcgccggcag cggctcggat cgagactctg taccgggaag tctcggacgg
     6301 tctgttcgct gccgggcgcg atttcgcgta tgtggatggc cgcgcattgg ccgaagccaa
     6361 agtggctggc ggcgcgctgg tgcatggcaa gctgcgctgg cgggtagtag tgctgccggg
     6421 cgcggataca ctcccgctag cagcctggga aaacctggcg cgctttgtcc gcagcggcgg
     6481 cgtgctgatc tccgtcggcg atcttccggc caacagcgag gccgagttcc catcgccgcg
     6541 cgtgcaagcg cttgtcaggg atctattcgg cccgggtgaa ggcgggccac gcgtctcggc
     6601 taagcgccac ggtggcgggg ccatttttct gcccggcagc agtgcggcgt tattgccgac
     6661 ggtcatcagc caggtcctgg cgccggacgt ggaagtggcc gggacccact ctccgatccg
     6721 tccaactcat cgccggatca acggacagga agtctatttc ctgatcaacg atagcgcgca
     6781 gccgtggagt gggcgcgtgg ggttatccgt tgatggaccg ggcgagtatg gcgatcctgg
     6841 cacgggccag gtgacgccgc tggcgagcgg gagcgacgtt cccctaagcc tgccgtctta
     6901 tggcgctgcg ctttttcgct tcccgaagac gcgcccgccg aagcttctgg gtttcggaaa
     6961 aaacgtgctt ccgcaattag ccaccagcga tctgcccgag gcgtcgccga aggttgcctg
     7021 cggggaattc gttcgcgagc aactcacgcc ggaagccgcg cctcgcggcg aagggcgtcg
     7081 ggcctggcag gcggttggcc ggctgacgaa aagccaggtt gataccttct tgttcgtgcg
     7141 gtttccgtat gccgacgggt tggacctgcg gaaggcagaa tggcttgtct tggacacgtg
     7201 ggttccgcca gcccagcgca cgccgaccca gcttctggtc gttctccgcc agaagaacgg
     7261 cgccgagtat ctggccagca ccggccgtta cttgggcgcg gccgggtatg agcaaacctg
     7321 cctgccgttg agccgattcc aattggccgg ctggtccaac gaccccaacg ggcggctcga
     7381 tccgtccagc gtcacggaaa tccgcgtcgg atggggcggt tactttggaa gcgagggcga
     7441 gaaggtcgag ttcactttgt gcgctccgag aagtgcgacc acgcaataag cgggttgatc
     7501 cctgcgccgc ggtgagatgc gcccacggcc ggaggtgccc cgctattcaa gctccagcac
     7561 gaccaccgat ttcgccggca gcgtggcagc aaagccaccg gaagcaatcc gcgccccggc
     7621 gaactcagcc gttttgaggg tctccggctc ttcaaaggta ttgtgtgcat tcatggccgg
     7681 agctgtcagg atacgacctg acaccttggt ggctttcgca ccctgcaagt cacacgcgac
     7741 ctcagccgca ttcttcgggt ccaggttgca caaggtcaca tgaatccgcc ccgcctgatc
     7801 gcgggaagcg gaaacgttca cctggggcag cttgtccgag ccagactggt aatccgccga
     7861 ttgcacctcg gagggaagca gcttggcgtc atgatgcacg gtgtacatct cgaatacgtg
     7921 atacgtcggc gtgaggatca tcttctcctt gtcggtcagg atcatcgcct gaagcacgtt
     7981 aatcatctgg gcgatgttgg acatctttac gcggcggcag tgattgttga agatgttcag
     8041 cgtcacaccg gcaaccagcg cgtcgcgcag tgtgttctgc tgatagagaa agcccgggtt
     8101 agtccccggc tccaccaggt gccaggtgcc ccactcatcc acgatcagtg ccaccttttg
     8161 gtcaggatca tacttatcca tgatctcgga atgcttcgtc accaactcct ccatccggag
     8221 cgcgcgctgg agttgggcga accagtcgcc ctcgtcaaat tccgtcgcaa gctgcgtctt
     8281 ctttccgctg ccgcagtagt agtgcaaagc cagcccggcc atgcgcttgc cggcttcgcg
     8341 catcagcgtc tcggtccact gatagttcgc gccattgggc ccacaggcaa ccttgtaaac
     8401 ctggttgccg ccgaaattcc ggacataggt agcgtagcgc ttgtattggt cggcataata
     8461 ttggggtgtc atgttcccgc cgcagcccca attctcattg ccgatcgcga agaacttcaa
     8521 cttccagggt tcagggtgac cgttctgctt gcgcaactcg gccatcgggc tcacgccgtc
     8581 gaaagtgatg tactccaccc actgctgcat ttcctgcacc gagccgctgc ccacattgcc
     8641 gcaaaggtag ggctcgcacc caagctgctc gcaaaggtcc aggaattcgt gggtgccgaa
     8701 atggttgttc tccaccaccc cgccccagtg agtattgatc atggtcggcc gttgagaccg
     8761 cggcccgatg ccgtctttcc aatgatactc gtcggcaaag catccgcctg gccatcgcag
     8821 gacgggaacc ttgatcttct tgagcgcggc caccacgtca ttccgaatgc cccgggtatt
     8881 ggcgatcgaa ctgtcctcgc ccacccaaat cccttcataa atacaccggc ccaggtgctc
     8941 ggaaaagtgg ccgtaaatgt tgcggctgat cgtgtctttc gcctggtcgg cgtggatcac
     9001 cagcttgctg gcggtcgccg tgctgttctc agctccccct gcaagctgcg ccgtgagcgt
     9061 gccggtgagt agaatggatg caagcagtcg tttgccgttc atgttgctca tttgacgaca
     9121 aagcagcaga tgctggcaag gtttctttag cctctaagtt ttgaaacgtc ctctggctga
     9181 agccgcccgt tgcgctaaga gcctgtttaa aaactcggtt tggagtcccg cctttaggcg
     9241 g
//
LOCUS       DATCGS010000303         4191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_422731, whole genome shotgun sequence.
ACCESSION   DATCGS010000303 DATCGS010000000
VERSION     DATCGS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422731"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VIL39_06170"
     CDS             <1..1130
                     /locus_tag="VIL39_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5481047.1"
                     /translation="LLVVLANNLGVMWFAVEATALSSVLLVALYNRRTSLEAAWKYII
                     LGSLGLALALLGTVFLYAAAVGAHASEKLPNFNWSHLMIVAPQFDHGLIKLAFVFALV
                     GYATKAGLAPMHTWLPDAHSEAPSPTSAMLSGVSLKIALYALLRFHILTTACLHSDFS
                     RYFLLGLGLFSMFLAAPFILVQKNLKRMLAYSSLEHVGLICTGFAMNSPVTIFGALLH
                     MGYHALTKPVLFFGAGNIHQTFHSLEFRVIGPGVVKVLPVTVLLMGLAALAAIGLPPF
                     GLFLSELTIVIGGLESQQTTVSVLILVALLASFCGILQQLTRILLGARKPGDPARDVR
                     HMDGVPAMALLLGGLLLFSVWLPAPLLELLHQAAAIIGGTP"
     gene            1127..2854
                     /locus_tag="VIL39_06175"
     CDS             1127..2854
                     /locus_tag="VIL39_06175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5481048.1"
                     /translation="MNILLELQPALADLTERFGGRIEAVHVARPNEVYFHAQMELVPG
                     FCAQLYKKWNARLVSLFADDARSLPNGVRPSPGAAASARSDAPERPQSSFAVDLAAPE
                     DGRTPAVKAGSGVFHLYYVFALDAAHGFFILRVPVSPAQPQFTSLTNALPAVNWQERE
                     IQDLFGIKLEGHPNPRRCALHDDWPEVYPLRKDFDLHTVLPPFTGERHKFRRVEGEGV
                     FQVPVGPVHAGIIEPGHFLFSVAGEPVLYLQIRLFYTHKGTEKLFENIPVTHGVRLAE
                     SISGDSSFAHATAFCHAVERAAKVEAPPRGRALRTVCLELERVYNHVADIGAIANDVA
                     FVVGNTHAMRLKERVLRLNEQLTGNRLLRGMAALGGVRFDWDAAQIEAIRQFVTELRP
                     EFESLVGLIRESSSTRDRLETAGILKPETARDLGVVGIAGRASGLDRDLRRDFPHAAY
                     DGVKFSVPVYREGDVLRRMQVRIDEVPAALSIIEQVISTLPDGPTRVLVPTPPANQVA
                     LGYVEGWRGEIFHWIRTAEDGRLARCKIKDPSLQNWPALSEAILGNIVPDFPVVNKSF
                     NLSYSGTDR"
     gene            2858..3700
                     /gene="nuoB"
                     /locus_tag="VIL39_06180"
     CDS             2858..3700
                     /gene="nuoB"
                     /locus_tag="VIL39_06180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY5481049.1"
                     /translation="MFDILRKSFATGIVTTAYPLARAEVSSQARGRPEVDFTNWKDAR
                     PAVAACPTGALACADSGETRSVTLDLGKCTFCGLCAEADAAIRMTNVFELAARRKEDL
                     VTTARYELGSGGTHTKLLCPQLAALNPQPPPLEAVGAEVKARVDKVLGRSLHIREVDA
                     GSCNGCEVEIVGLNSPVYDIERFGIHFVASPRHADMLLVTGPVSRNMELALRKTYDAT
                     PAPRLVVAVGACGCSGGIFGQNYASLGGVDKVIPVDVYIPGCPPNPHALLHGILLALG
                     RLRL"
BASE COUNT          658 a         1378 c         1294 g          861 t
ORIGIN      
        1 tgttgctcgt cgtgctggcc aacaatctcg gcgtgatgtg gtttgccgtg gaagccaccg
       61 cgctgtcatc cgtcctgctc gtcgcgctct acaaccgccg gacttcgctc gaagccgcct
      121 ggaaatacat catcctcggc agcctcggcc tggcgctggc actgttgggc acggtgttcc
      181 tatacgcggc ggccgttggc gcgcacgcgt ccgaaaagct gcccaacttc aactggtcgc
      241 acctgatgat cgtcgcgccg cagttcgacc acggccttat caagctggcc ttcgtgtttg
      301 cgctggtcgg ctacgccacc aaggccgggc tcgcccccat gcatacctgg ctgcccgacg
      361 cgcacagcga ggcgccctca cccaccagcg cgatgctttc gggcgtgtcg cttaagattg
      421 cgctttacgc cttgctgcgg ttccacatcc tcaccacggc ctgcctgcac tcggacttca
      481 gccgctattt tctcctgggc ttgggcctgt tctccatgtt cctcgccgcg ccgttcattc
      541 tcgtgcagaa gaacctcaaa cggatgctcg cctattcgag cctcgaacac gtcggtctca
      601 tctgcaccgg ctttgccatg aactcgcccg tgaccatctt tggcgcgctg ctgcacatgg
      661 gctatcacgc gctgaccaaa ccggtgttgt ttttcggcgc gggcaatatt catcagacct
      721 tccactcgct cgaattccgg gtcatcggcc ccggcgtggt gaaggtgctg ccggtcaccg
      781 tgctcctgat gggactggcc gccctcgccg ccatcgggct gccgccgttc ggcctgttcc
      841 tgagcgagtt gaccatcgtc atcggcggcc ttgagtcgca gcaaacgacc gtcagtgtgt
      901 tgattctggt ggcgctcttg gcttcgttct gcggcatcct gcagcaactc acccgcatcc
      961 tgctgggcgc gcgcaagccg ggcgaccccg cccgcgacgt ccgccacatg gatggggtgc
     1021 cagccatggc gctgcttctg ggcggtctgc tgctctttag cgtgtggctg cctgcgccgc
     1081 tgctcgaatt gctgcaccag gcggcggcca ttatcggagg taccccatga atatcctgct
     1141 tgaactgcaa ccagccctcg cggatttgac tgaacgcttt ggagggcgaa tcgaggcggt
     1201 gcatgtcgcc cgtccgaacg aggtttattt tcacgcccaa atggaactgg tcccgggttt
     1261 ctgcgcccag ctctataaga agtggaacgc gcggctggtc agcctgttcg ccgatgatgc
     1321 ccgttccctt cccaacggag tgcgcccgtc cccgggcgca gcagcctccg cccgatccga
     1381 cgcgccagaa cgtccccaga gctcgttcgc cgtggacctt gctgcgcccg aggacgggcg
     1441 cactccggcg gtgaaggcag gcagcggcgt gtttcacctc tattacgtgt ttgcgctcga
     1501 tgccgcgcac gggtttttca ttttgcgcgt gccggtgtcc ccggcgcagc cccaattcac
     1561 gtccctcacc aatgccttgc ccgccgtcaa ctggcaggag cgggagatac aggacctgtt
     1621 cgggattaag cttgagggtc atcccaatcc tcgccgctgc gcgctgcatg atgactggcc
     1681 ggaagtgtat ccgctgcgta aggacttcga tttacacacc gtgctgccgc cgttcaccgg
     1741 cgagcggcac aagttccgcc gggtcgaggg cgaaggcgtc tttcaagtcc cggtggggcc
     1801 ggtgcatgcg ggcatcattg agcccggcca cttccttttc agcgtggctg gggagcccgt
     1861 cctctacctc caaatccggc tcttttacac gcacaagggc acggagaagc tgttcgagaa
     1921 catcccggtg acccatggcg tccggctggc ggagagcatt tccggcgatt ccagctttgc
     1981 ccacgcgacc gccttttgcc atgcggtcga acgagctgcc aaggtggaag cgccgccgcg
     2041 cgggcgcgcg ctgcggacgg tctgcctgga gctggagcgg gtttacaacc atgtcgccga
     2101 catcggggcc attgcgaacg atgtggcgtt cgtcgtgggc aacacccacg ccatgcggct
     2161 caaggagcgg gtgctgcgcc tgaacgaaca gctcaccggc aaccgtctcc tgcgcggcat
     2221 ggccgcgctc ggcggcgtcc gtttcgattg ggacgccgcg caaattgagg ccatccgcca
     2281 gtttgtgact gagctgcggc ccgagttcga gtcgttggtt ggcttgattc gcgagtcctc
     2341 ttccacgcgc gaccggctcg aaaccgccgg catcctcaag cctgagaccg ctcgtgatct
     2401 cggcgtcgtt ggcattgcgg ggcgcgcgtc cggcctcgac cgcgacttgc ggcgcgactt
     2461 cccgcacgcc gcttacgacg gcgtgaagtt cagtgtgccg gtctatcgcg agggcgatgt
     2521 gctgcggcgg atgcaagtgc gcattgatga ggtgcccgcg gcgctctcga ttatcgagca
     2581 ggttatcagt acactccccg atggcccgac gcgggtgctc gtgccaacgc cacccgccaa
     2641 ccaggttgcg ctcggctacg tggaagggtg gcgcggggaa atcttccact ggatccgcac
     2701 ggctgaggat ggccgcttgg cccggtgcaa gatcaaggac ccgtcgctgc agaactggcc
     2761 cgcgctgagc gaggccatcc tcggcaacat cgtcccggat tttcccgtgg taaataagag
     2821 cttcaacctg tcttactccg gcaccgaccg gtagattatg ttcgatattc tgcgcaagag
     2881 ttttgctacc ggcatcgtga ccaccgctta tccgctggcc cgggcggagg tgtcgagtca
     2941 ggcgcgcggg cggccggagg ttgatttcac caattggaag gacgctcgac ctgccgtcgc
     3001 ggcttgtccg acaggcgctc tcgcgtgcgc tgacagcggc gagacgcggt ccgtgacgct
     3061 ggaccttggg aagtgcactt tctgcggctt gtgcgcggaa gcggacgcgg ccatccggat
     3121 gacgaatgtt tttgaactgg cggccaggcg caaggaggac ttggtgacca cggctagata
     3181 cgaactcggc tccggcggca cgcacaccaa gctgctgtgc ccgcagctcg cagccctcaa
     3241 ccctcaaccc ccacctctgg aggccgtcgg cgcggaggtt aaggcgcgtg tggacaaggt
     3301 gctgggccgg tccctgcaca tccgcgaagt cgatgccggc tcgtgcaacg gttgcgaggt
     3361 tgaaatcgtg gggctcaaca gccccgtgta cgacatcgag cggtttggca ttcacttcgt
     3421 cgcgtcgccg cgtcacgccg acatgctgct ggtcaccggc ccggtttcgc gcaacatgga
     3481 actcgcgctg cggaagacct atgacgccac gcccgcgccg cgtttggtgg tggccgtcgg
     3541 cgcctgtggc tgcagcggcg gtatcttcgg ccagaactac gccagcttgg gcggcgtgga
     3601 caaagtcatc cccgtggatg tctatattcc cggttgcccc cccaatcccc acgccctcct
     3661 gcacgggatt ctgctggcgc ttggcagact cagattatag ggctgaacgc cggaagcgcg
     3721 ggaagcgcag ccttccggcc cggagccgtg tgtctcatcc gcttatggaa ttgaacgaat
     3781 ccacgtagcc ttggcaaagc gcggcgacag cgtggcaaag gccaggagca agccgggaga
     3841 tcaggaagtg tcctcgtatt ctcttcgtat tcccctcgta ttctcttcgt attctcttcg
     3901 tgtttaccac ttgttgtctg gtggtgtccc tcgccgcccg tcgtcggctc caccaagccc
     3961 ctggcttcca ggttggtcca ctatgtaaat ggactcgcag tcgtccagaa agtgagatac
     4021 gaccggcagc agccggaccc gcccgtcagg cctgacagct cgataagctt gcagcgccag
     4081 aggctttggc ttccggctcg gcccatttgc cgtgctcccg gatgaggtcc accaggcggt
     4141 ccacggcctc cgcttcggga atattgaact tcaccgccgt cttgccgacg t
//
LOCUS       DATCGS010000304         4954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_670812, whole genome shotgun sequence.
ACCESSION   DATCGS010000304 DATCGS010000000
VERSION     DATCGS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670812"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIL39_06185"
     CDS             complement(<1..224)
                     /locus_tag="VIL39_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY5481050.1"
                     /translation="MRLLDRYLLRELIVPLGYCLWGFLLLWISSDLFRTLGDFQKQGL
                     VARDISEYYLVKLPEFLVLVLPIALLLAML"
     gene            complement(400..3204)
                     /locus_tag="VIL39_06190"
     CDS             complement(400..3204)
                     /locus_tag="VIL39_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5481051.1"
                     /translation="MRILPRISISTGCRFGALILLASLPAGCQSPSAPVAQDSTHDII
                     GRIGENRYYTPANQILTPAGLQVELPGMRPQALALSPDGRLLVTAGKTHDLVVVEPRS
                     GKVLQRVLLPSEKDQDPALQPVSDHILQPDKDGQISFTGLAFSPDGSRIYLANVDGSI
                     KVFGIEKGGRVVGLFTMPLPPANAPRRAAEVPAGLAVYQDGKRLYVTLNLSNRLGELD
                     TATGKVLRLWNVGIAPYDVVLAGHKAYVSNWGGRRPETNSVTGPAGRGTLVRVDPVRY
                     IASEGSVSVIELATSRQVVGQASRLPSGRLAPGSSAGETPAQAAETAAPLTEILTGLH
                     ASAMVVSPNGRWVVAANAGSDTLSVIDTRSDRVTETLCARQTPADLFGAQPNALAFDK
                     SGKRLFVCNGTQNAVAVFDFSPGQSKLLGLIPVGWFPGAVVHDPRRKALYVANLKGIA
                     STMTLRPNERAGYNSHQYRGSLSLVKVPSARNLAAYTSAALANMRYPLLREAALPPRP
                     GQPLRPVPQRVGEPSVFKHVVYIIKENRTYDQVLGDMKEGNGDASLCVFGERITPNQH
                     KLAREFVLLDNTYCSGLLSADGHQWADTALATDYMERSFAGFPRSYPDGMEDDDVDAL
                     AYSPGGFIWDNVIAHGKTLRDYGEFAISDARWKDPARKGAPKFLDCYRNFTSQTGEIE
                     IRSRPAIESLRPYLVTNTAGWDLSIPDVFRAAQFIGELKQFEQSGAFPNLSLICLPND
                     HTSGTNPGSPTPDAQVADNDLALGQIIEAISRSRFWPETCIFVIEDDPQNGWDHISGF
                     RTTAYVASPYTRRGATVRTQYNQTSLLRTMELILGLPPMNQMDATATPMSDCFTQTPD
                     LAPFTAVPNNIPLDRMNPEPKKLSDPLLRKNAYASARLPLDQPDQCPEDMLNRILWHA
                     MKGSQAPYPEWAVRTQPDED"
     gene            3717..4784
                     /locus_tag="VIL39_06195"
     CDS             3717..4784
                     /locus_tag="VIL39_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481052.1"
                     /translation="MNENLIFGLFLLAPCVCGAGLLLFARQVRRRPGPVPWDSLILGN
                     ALTLLCLLSIGFTAGEAYFRFVYDTTDSLDCTRVCHRWAERYWRVNAWQCRDDLQYAF
                     QVQPGKRRVTFLGDSFTTGHGIKHTEDRFGNRVRRAHPEWEIHLLARNGFDTGNEIAG
                     LKEVLARGYQLQDVVLVYCLNDVADMQPEYSQMLAQVAADVARAGWLRRNSYLADFLY
                     CRYRALRDPRLKDYYPFVRDAYRGPLWEQQKQRLKILRDLVRSQGGRLSVVTFPFLHA
                     LGPAYPWQFVHDELGQLWRDLNVPHLDLLPVFANLPPARLTVNRFDAHPNELAHRLAA
                     EEIDRFLSDTVKPVSPASGAN"
BASE COUNT          865 a         1523 c         1623 g          943 t
ORIGIN      
        1 tagagcatcg ccagcaacag cgcgatcggc agcaccagca ccagaaactc cggcagcttg
       61 accaggtaat actctgagat gtcccgcgcc accaagccct gcttctgaaa atcgcccagc
      121 gtcctaaaca gatctgagga gatccaaagc agcagaaagc cccacaggca atagcccagc
      181 gggacgatca gttcgcgcaa tagataacgg tccaggagcc gcattccgcc gcagcgtaac
      241 taccactccg tcgcaaagca agtaactgcg actttcacca cgtcagagcg gcagcttgtg
      301 catggccggc ttccgcgggc agatcggcgg cgcatccacg atggtaatcg tgtagctgtc
      361 ctcgaagctc gtgccgcccg ccgggcagac ccccgcatat tagtcctcgt ccggctgagt
      421 ccgcacggcc cattcaggat agggagcctg ggagcctttc atggcgtgcc agaggatgcg
      481 gttgagcatg tcttcggggc attggtcggg ctggtcgagc ggcaagcggg cagaggcgta
      541 ggcgttcttg cgcaggaggg gatcggagag cttcttgggc tcggggttca tccggtcgag
      601 cgggatgttg ttgggcacgg cggtgaaggg cgcgaggtcc ggcgtctgag tgaaacagtc
      661 ggacatgggg gtggcggtgg cgtccatctg gttcatcggc ggcaggccga gtattaattc
      721 catcgtgcgc aggaggctgg tctggttgta ctgcgtgcgg acggtcgcgc cgcgccgcgt
      781 gtatgggctg gcgacgtagg cggtggtgcg gaagccgctg atgtggtccc agccgttctg
      841 ggggtcgtcc tcgatgacaa agatgcacgt ctcgggccag aaccggctgc ggctgatggc
      901 ctcgatgatc tggccgaggg cgaggtcgtt gtcagccact tgcgcgtcgg gcgttgggga
      961 acctgggttg gtgccgctgg tatgatcgtt gggcaggcag atgaggctca ggttcgggaa
     1021 agcgccgctc tgctcgaatt gcttcagttc accgatgaac tgcgcggcgc ggaatacatc
     1081 cgggatactc aagtcccacc ctgccgtgtt cgtcacgaga tacggacgca gtgattcgat
     1141 ggccgggcgg ctgcgaatct cgatctcgcc ggtttggctg gtgaaattgc ggtagcaatc
     1201 aaggaactta ggcgcgcctt tcctcgctgg atccttccac cgtgcgtcgc tgatggcgaa
     1261 ctcgccatag tcgcgcagcg tcttgccgtg ggcgatgacg ttgtcccaga tgaacccccc
     1321 aggcgaatag gccagcgcgt ccacgtcgtc gtcttccatt ccatccgggt aactgcgcgg
     1381 gaacccggcg aaggaccgtt ccatgtagtc ggtcgccagg gcggtgtcgg cccactggtg
     1441 gccatcagcg ctgaggaggc cggagcaata cgtgttgtcg agcaacacga actcgcgtgc
     1501 cagcttgtgc tggttgggcg tgatgcgttc cccgaacacg cagagggaag cgtcgccatt
     1561 gccctctttc atgtcgccca gcacctggtc gtaagtgcgg ttctccttga tgatatagac
     1621 gacgtgcttg aacacgctcg gctcgccgac ccgctgcggc acgggtcgca gcggctggcc
     1681 cggacgcggc ggcagggcgg cctcacgcag cagtggatag cgcatgttgg ccagcgcggc
     1741 gctcgtgtaa gccgccagat tacgtgccga gggcactttc accagcgaaa gcgagccgcg
     1801 atactggtgg ctgttgtagc cggcacgttc gttcggtctg agcgtcatgg tcgaagcaat
     1861 gcctttgaga ttggcaacgt aaagcgcctt ccgtctcggg tcgtgaacga cggcgccggg
     1921 gaaccagccg acggggatca ggccaagcag cttcgattgg ccgggggaaa agtcgaacac
     1981 cgcgacggcg ttctgggttc cgttgcagac gaaaagcctc ttgccagact tgtcgaatgc
     2041 cagcgcattc ggctgagcgc caaagaggtc cgccggggtt tgccgcgcgc agagggtctc
     2101 cgtcacccgg tcgctccgcg tgtcaatcac actgagcgtg tcgctgccgg cattggcggc
     2161 gaccacccag cggccgttgg gcgacacgac catcgcggag gcgtgcaggc cggtaaggat
     2221 ctcggttagt ggggcagccg tctcggctgc ctgggcaggc gtctcgcccg cgctcgagcc
     2281 gggggcgagt cgcccggagg gcaggcgaga cgcctgcccc actacctgtc ggctggtggc
     2341 cagctcgatc accgacacgg aaccttcgct ggcgatgtag cgcaccgggt ccacgcggac
     2401 cagcgtgccg cgcccggcag ggccggtgac actgttcgtc tcgggccgcc ggccacccca
     2461 gttgctgacg taggccttgt gcccggcgag cacaacatcg tagggggcaa tgccgacgtt
     2521 ccagagacgc aagaccttgc cggtggcggt gtccagttcg ccgaggcgat tcgagagatt
     2581 gagcgtaacg tacaggcgtt tgccatcctg gtacaccgcg aggccggcgg ggacttccgc
     2641 cgcgcggcgc ggggcgttgg ccggcggcag cggcatggta aatagaccca ccacccggcc
     2701 gcccttctcg atgccgaaaa ccttgatact gccatccacg ttcgccaggt aaatgcgcga
     2761 gccgtccgga gaaaaggcca gcccggtgaa actgatctgc ccgtctttgt caggctggag
     2821 gatgtggtcg gagacgggtt gaagcgcggg gtcctggtct ttctccgatg gcagcagcac
     2881 ccgctgaagg actttcccgg accgcggctc gacgacaacc aggtcgtgcg ttttgcccgc
     2941 ggtcaccagc aaccgtccat cggggctcag cgcgagcgcc tggggacgca tgcccggcag
     3001 ctcgacctgc aacccggcgg gcgtgaggat ttggttggcc ggagtgtagt agcggttctc
     3061 gccgatgcgc ccaatgatgt catgggtgga atcctgtgcg acgggcgccg acgggctctg
     3121 gcagcccgcg ggcaggctcg caaggagaat cagcgccccg aaccggcaac cagtagagat
     3181 tgaaatccga gggaggattc tcatgcgtta ttgcaggaag gcgcctgcca gcttaaacac
     3241 tttggttgca gcaattccat gcaaatagct gctcggcttt aagccgtttt gtgcgtcggc
     3301 acaagccaaa agatgccgcc ggttctggag caaccgggtc agggcgccgg aatactcgtt
     3361 cggtaaagct ctactccctg gtcattaaaa gtgagcacgg gcatttgcca cggaataccc
     3421 ccacgccatc gaactctccc gcgccctcgc cgccgagatc ttgaaactgg agcgcacgct
     3481 cagcagccgg cagacattgg cgcttgttag gctcatcctc ggctggcaag ttgtgcgcaa
     3541 gacgaatgcg agcgagaacc aattccccct cacccgcgca gcgacaagat gagcgtcgcg
     3601 gtgaaggcgc agttttcgag caggcggcct cctattggat gcctcggctg ttgtccgctg
     3661 gcgccgattg tcggcgcagc actggctgtg gttgtcgccg ggcgtgactt ccgtgcatga
     3721 atgaaaacct gatattcggg ctttttctgc tcgcgccctg cgtctgcggc gcgggtttgc
     3781 tgctctttgc ccgccaggtg agacggcgcc ccggcccggt tccctgggac tcgctgattc
     3841 tgggcaatgc ccttacgctg ctctgtctcc tgtccattgg gttcaccgcc ggggaagcct
     3901 atttccggtt cgtttacgac acaaccgact cgctggactg cacgagggtt tgccaccgct
     3961 gggccgagcg ctattggcgg gtgaacgctt ggcaatgtcg ggacgacctc cagtacgcct
     4021 tccaggtcca gccgggcaag cgacgggtga cgttcctggg cgattctttc accaccggcc
     4081 acggcatcaa gcacaccgaa gaccgctttg gcaaccgcgt ccgccgggct cacccggaat
     4141 gggaaatcca cctgctcgcg cgcaacggct tcgataccgg caacgagatc gccggcctca
     4201 aagaggtgct ggccaggggc tatcaactcc aggacgtggt cctcgtctat tgccttaatg
     4261 atgtcgcgga tatgcagccg gagtactccc agatgctggc ccaggtcgcc gcggacgtgg
     4321 cccgcgccgg ctggcttcgg cgcaacagtt atttggccga cttcctctat tgccggtacc
     4381 gggcattgag ggatcctcgc ctgaaagact actacccctt cgttcgcgac gcctaccgtg
     4441 ggccgctgtg ggagcagcag aaacagcggc tcaaaatcct gcgcgacctc gtgcggtcgc
     4501 agggcggtcg gttatcggtc gtcaccttcc ctttcctcca tgccctgggg ccggcttacc
     4561 cttggcagtt tgtccacgat gaactgggcc aactctggcg ggatctgaac gtgccgcacc
     4621 tcgacctcct cccagtcttc gccaacttgc ctcccgcccg gctcaccgtc aaccggttcg
     4681 acgcccatcc aaacgaactc gcccaccggc tcgccgccga ggaaattgac agattccttt
     4741 ctgacaccgt caaacctgtg tcccctgcgt ctggcgcgaa ttagatggcg aagaggacac
     4801 ccgcagacga ggacgggtaa tgtcacgaag attctgtggc ctgatcacgg gcacgggcgg
     4861 cggctctgct tcctctgagg ggtgggaact ttagtcttcg gtgccgcacg cggcggactt
     4921 cgctgggtcg ttctttgcgt cgcttaggca gggt
//
LOCUS       DATCGS010000305         8384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_372065, whole genome shotgun sequence.
ACCESSION   DATCGS010000305 DATCGS010000000
VERSION     DATCGS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372065"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="larC"
                     /locus_tag="VIL39_06200"
     CDS             <1..1137
                     /gene="larC"
                     /locus_tag="VIL39_06200"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEY5481053.1"
                     /translation="KRAHKASLEGVKFDVHLAEEHDHDAAPHAHHHAHAHSHAHTHEH
                     GRNFAQIRQLITASQLSEWVKRKSESVFQRLAVAEGRIHGLPPEQVSFHEVGAVDSIV
                     DIVGACIALELLGKPRVLASAVVEGMGWIDCAHGRLPIPAPATLAILGARGIALSQCD
                     EPNELVTPTGAALLAEFVDAFGPMREVVAEKIGYGLGTRDNPRRPNALRAILGEAGAQ
                     GQGPGAGAGGHDWETDTVVVLETNLDDINAEILGNFMEKALAAGALDVFHTAIQMKKN
                     RPGVLLTVLCAETDADKFSELLLRETSAFGIRRSTAERRKLRREFITVQTPHGSVTAK
                     LGKLDGKVLQAAPEFESCKKLAEQANVPIKEVYEAALRALPPSL"
     gene            1140..1391
                     /locus_tag="VIL39_06205"
     CDS             1140..1391
                     /locus_tag="VIL39_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5481054.1"
                     /translation="MIDPELLKILCCPETHQELRLAEPAVIEKLNGQIAAGTLSNRAG
                     QPVKEKIDGGLIRADGKLLYPIRRNLPVMLVDEGILLAG"
     gene            complement(1548..3266)
                     /locus_tag="VIL39_06210"
     CDS             complement(1548..3266)
                     /locus_tag="VIL39_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY5481055.1"
                     /translation="MKLSTSQSLAGILEPVFAIRTEEDLGIGDTEGVRQMIDWCARHR
                     LNILQTLPINELSDDNSPYNALSSLAIEPATIAISPQHIPDLTPGRFKELAGPELLAE
                     LRQGPVNYLAVKALKRKLLEAAFENFIEQHYIPETERALQFRSFQVENGGWLSDYSLF
                     RVLMEENGGFPTWDCWAPEHRSPRTARAWLLSLPVKRSVELIHKQLFFMYVQWLAFGQ
                     WQALKAYGTAKKVYLMGDIPFGVGRFSADVWANHSIFDLDWSGGAPPEKVFKVDPFTE
                     KWGQNWGIPNYRWDELRRRDFDWWRTRVGNIQKVFHLYRIDHVLGFFRIYSFPWTPDR
                     NAEFLPLTEAQAAARAGGRLPGFKQFPDDTAEHKAVNQAQGEEILRVVLEASGETTVV
                     AEDLGVVPEYVPPTLHKLNTPGFRIPTFFRDPDGRYSDPKNYPRLSLVQPATHDHPPL
                     AAIWAECWQNIDAGKNVETNRRELRLMMEFSGLKDSEPPREFTDQLHEAYTRVVLQSR
                     SWLAIFQITDVFGMTARFNTPGSVASTNWSYRLPHTVEELDEDPLLLAQTEMFSRLAQ
                     ESGRVG"
     gene            complement(3523..4419)
                     /locus_tag="VIL39_06215"
     CDS             complement(3523..4419)
                     /locus_tag="VIL39_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEY5481056.1"
                     /translation="MFSTLNKWLSFVRFSHTVFALPFALAAMAVAARANRGWPGWRTL
                     ALILVAMVCARTCAMAFNRIVDRKFDALNPRTAGRHLPTGQISLLSAVTLCVLSGAGL
                     VGASFLLNSLCFYLSPVALIVICFYSLTKRFTDYTHVYLGLALALAPVGAWLAVKGMD
                     ISGWEIVQMLLLAMVVVLWLVGFDIIYAIQDYEFDRAHGLHSLVVAWGPKNALQAAFL
                     AHLVMCGLLLAFGLLCKFRIAYIIGWLLIVGCLVLEHWIARRRSLNWINLAFFRLNAV
                     ISVIFLTVTLAEVIFLGGFRLR"
     gene            complement(4465..4974)
                     /locus_tag="VIL39_06220"
     CDS             complement(4465..4974)
                     /locus_tag="VIL39_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY5481057.1"
                     /translation="MKRPKHPTSNIQHPTAIEEASEVWEFREGETTQRRFDLEERLLE
                     FASAVIDLSEKLPDTRAGNHIAGQVLRSGTSPYPNHGEAEDAESRDDFIHKLKICLKE
                     LRETRRWARLIRRKAWATDDPTLVFLLSEADELIRIFYSSVQTARRNALAERRAAPDP
                     TRYPPRKSI"
     gene            complement(4983..5540)
                     /locus_tag="VIL39_06225"
     CDS             complement(4983..5540)
                     /locus_tag="VIL39_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY5481058.1"
                     /translation="MRILVAITGASGSLYAQRLLDNLDPAQHQIDVVLSRYAPDVIAE
                     ELPGGLRLPAGAVSHSLKSMNAPFASGSSAPDAMVIIPCSMGTLGRIAHGYSEDVLLR
                     AADVVLKERKKLILVPRETPLNLIHVKNFELLIQAGATILPANPSFYTRPQTIEQVVD
                     TVVARVLDHLGIPNKLTPRWAGEKE"
     gene            5694..6953
                     /locus_tag="VIL39_06230"
     CDS             5694..6953
                     /locus_tag="VIL39_06230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5481059.1"
                     /translation="MPGLAGGGSDRPELFTACLFCLLLAVVLAGCATGPRENGTQAPN
                     WEREDVATASVTPVAPVTPVAPPAPAPVATPPTSAAPTNQPAETWIPLDRWCKANGLA
                     APCLVGPAPAPAYALIMPDGVVVLRAGSLVAHWDRLDVRLGFAPQMMNGQPFVHALDL
                     KKTLEPLVRGSCLSFLKTNPMIVIDPGHGGQDSGTKGVLGYRYEKDFTLDWARRLGSL
                     LATNGWQVFLTRSNDMDLSVSNRIVFAEAHKADVFLSLHFNSSAPNVQQAGLETYCLT
                     PTGMPSTVTRGYNDNQALAFPNNAFDAQNLQFALQVHRALLQVNGGHDRGVRRARYLG
                     VLRGQNRPAILVEGGYLSNPSEARRIANPAYRQELAEAVAKALAVKSNVQSPRSEVAS
                     QGREPGSQRAETRIQKPEATTTNIATP"
     gene            6950..7936
                     /locus_tag="VIL39_06235"
     CDS             6950..7936
                     /locus_tag="VIL39_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5481060.1"
                     /translation="MSTHHVSRFTSHVLVTGGAGFIGSHLVERLLADGKSVVVVDDLS
                     TGRLENLQAVRAHPKLRVIESRISSCPELGELVTQAEAIYHLAAAVGVELVVRSPIHV
                     LETNLHETEVLLKAAAGQGVPVLLTSTSEVYGKSQKEAFGEEDNLLIGPPHQVRWSYA
                     CSKLMDEFLAMAYAKERALPVIITRLFNTVGPRQTGQYGMVLPRFIAAARSGQPLRVY
                     GDGRQTRCFCYVRDTVEALVRLQGCVAARGQVFNVGSTEEISIRDLARKVIESLRSES
                     AIELVPYSQAYEPGFDDMRRRKPVVEKLDGTVGFRPRTGLQTIIELTAGALT"
BASE COUNT         1644 a         2458 c         2732 g         1550 t
ORIGIN      
        1 aagcgtgcac acaaggccag cctcgaaggc gtgaagttcg acgtgcacct ggccgaggag
       61 catgaccacg atgcggcccc gcacgcgcac caccatgcgc atgcgcatag ccacgcccac
      121 acgcacgaac acggccgcaa cttcgctcag atacggcaat tgatcaccgc gagtcagctc
      181 tccgaatggg tgaagcggaa gtcggagtcg gtgttccaac gcctcgccgt ggctgaaggc
      241 aggattcacg ggctgccgcc ggagcaggtg agcttccatg aggtcggcgc ggtggattcg
      301 attgtcgata ttgttggggc gtgcatcgcg ttggagctgc tgggcaagcc gcgcgtcctg
      361 gcaagcgcgg tcgtcgaggg catgggctgg attgattgcg cgcacgggcg tctgccgatc
      421 cccgccccgg cgacgctggc gatcctgggc gcgcgcggca tcgctttgtc gcaatgcgat
      481 gaaccgaacg agctggtcac gcccacgggc gcggcgctgc tggcggagtt tgtcgatgcc
      541 ttcggcccga tgcgggaggt ggtggcggag aagatcggtt atggcctggg cacccgtgac
      601 aatcccaggc gacccaatgc gctaagggcc atcctcgggg aggcgggcgc tcagggccag
      661 ggtcccggag ccggagcggg agggcacgat tgggagacgg ataccgtcgt agtgttggag
      721 accaacctcg atgacataaa cgccgagatt ctaggcaact tcatggagaa ggcgctcgcc
      781 gccggcgccc tggacgtgtt ccataccgcg atccagatga agaagaaccg gcccggcgtg
      841 cttttgacgg tgctgtgtgc cgagactgat gcggacaagt tctcagagtt gctgctgcgt
      901 gagaccagcg cgttcggcat ccgccgttca accgccgaac ggcgcaagct tcgacgggag
      961 ttcataaccg tccagacccc ccacggcagc gtcaccgcga agcttggcaa gctcgatggg
     1021 aaggtcctcc aagccgcgcc cgagttcgag tcctgcaaga agctggccga gcaggcaaac
     1081 gtgccaatca aagaggtcta tgaggcagcc cttcgcgcct taccgccttc gctgtgacta
     1141 tgattgatcc tgaactactg aagattctgt gctgtccgga gacgcaccag gagctgcgtc
     1201 tggcggaacc ggccgtcatt gaaaaactga atgggcaaat agccgcgggc accctctcca
     1261 atcgcgccgg ccagccggtg aaagagaaga ttgacggggg actgatccgc gctgacggca
     1321 agctgcttta cccgatccgg cggaacctgc ccgtaatgct ggtggacgaa ggcattctgc
     1381 tggcgggcta gctacagtta gatcctgtcg ggcgaatgcg agttctccct ggccggcaaa
     1441 ccgcacaacc tgaaggccgg cgatttcctg aacatgccgc cgaacctgtc gcacgcggcc
     1501 aaggcgacca agcagttttc gatgctgctg acgctcttca aaccggttta gcccacccgc
     1561 ccgctttcct gtgcgaggcg ggagaacatt tccgtttgtg ccagcagcag cgggtcttcg
     1621 tccagctctt cgacggtgtg cggcaggcgg taggaccagt tggtcgaagc cactgagccg
     1681 ggggtgttga agcgtgccgt catcccgaac acgtcggtaa tctgaaagat ggccagccag
     1741 gaccgcgatt ggaggaccac tcgcgtgtac gcctcgtgca gttggtcggt gaactcgcgc
     1801 ggcggctcgc tgtccttcaa tccggagaac tccatcatca ggcgcagttc gcgccggttc
     1861 gtctccacgt tcttgccggc gtcaatgttc tgccagcact ccgcccagat ggcggcgagg
     1921 ggcgggtggt cgtgggtggc gggttgcacg agggagaggc gcggatagtt cttcggatca
     1981 gaataccggc catccgggtc gcggaagaac gtggggatgc ggaagcctgg ggtgttgagc
     2041 ttatgcaatg tgggcggcac atactcgggc acaacgccca ggtcctcagc cacgacggta
     2101 gtctcgccgg aagcctccag cacgacgcgc aggatttcct cgccctgggc ctggttgact
     2161 gccttgtgct cggccgtgtc gtctgggaac tgcttgaagc cgggcagacg gccgccggcc
     2221 ctggccgcgg cttgcgcttc ggtgagcggc agaaactccg cgttccgatc cggcgtccac
     2281 gggaacgagt agatgcggaa gaacccgagc acgtggtcaa tgcgatatag gtggaagact
     2341 ttctggatgt tgccgacgcg agtgcgccac cagtcgaagt cgcgccgacg caattcgtcc
     2401 cagcggtaat tagggatgcc ccaattctgc ccccacttct cagtaaaggg atcaactttg
     2461 aaaaccttct ccggcggtgc gccgccgctc cagtcgaggt cgaagatgga gtggttagcc
     2521 cagacgtcgg cgctgaaacg ccccacgcca aaggggatat cgcccatcag atagaccttc
     2581 ttcgccgtgc cgtaagcctt gagcgcctgc cattggccga aggccaacca ttggacgtac
     2641 atgaagaaaa gttgcttgtg gatgagttca accgagcgtt tcacgggcag cgacaacagc
     2701 caggcgcgag cggtgcgcgg cgagcggtgc tcgggtgccc agcagtccca ggtgggaaag
     2761 ccgccgttct cctccatcaa cacgcgaaac agggagtagt cggacagcca gcccccgttt
     2821 tccacctgga acgagcggaa ttgcagggcg cgctcggttt ccggtatgta gtgctgctcg
     2881 atgaagttct cgaaggcggc ttcgagcaac ttgcgcttga gagccttgac ggcgagataa
     2941 ttgaccggtc cttggcgcaa ctcagccaac agctctggtc cggccagttc cttgaatcta
     3001 ccgggcgtta agtcaggaat gtgctgcggc gatatggcga tggtagcggg ctcgatcgcc
     3061 agcgaactca gggcgttgta ggggctgttg tcgtcgctga gttcattgat gggcagggtc
     3121 tgcaggatgt tcagccggtg acgggcgcac cagtcaatca tctggcgcac gccctccgtg
     3181 tcgccgatgc ccagatcctc ctcggtgcgg atggcaaaga ccggctccaa tatgccggcc
     3241 aacgactggc tcgtactcag tttcatatat cgtaggctgg aaacttgagc gcatcagccg
     3301 caccgcgtca agcgccacac ggcgagaatt cgcacaacag tagaggaaca atagagggca
     3361 ggtggctgct ccctggcttg attcacgctc gctccttgcg attccggccg gctgcccagc
     3421 gggacatctc tccagttacc ggctcgctcc tggctctccc cgcgcgccct ctgccgttca
     3481 cccaacgctt cgcttatcct ggcaccgagc tgagccagga tcttaacgca ggcgaaagcc
     3541 gcccaggaag atgacctcgg ccagggtgac ggtcaggaag atgacgctga tgacggcgtt
     3601 gaggcgaaag aaagcgaggt tgatccagtt gagactgcgg cggcgggcaa tccaatgctc
     3661 cagcacgagg cagcccacga tgaggagcca gccgatgatg taggcgatgc ggaatttgca
     3721 cagcagccca aaagcgagca ggagcccgca catcaccagg tgcgccagga aggcggcttg
     3781 cagggcgttc ttcggccccc aggcgacaac gagcgagtgc aggccgtgcg ctcggtcgaa
     3841 ttcatagtcc tggatcgcat agatgatgtc gaaaccgacg agccagagca ccaccaccat
     3901 ggccagcaag agcatctgga cgatttccca cccggagatg tccattccct tgacggccag
     3961 ccacgcgccg accggcgcca gggccagggc aagtcccaga taaacgtgcg tgtaatcggt
     4021 gaagcgcttg gtcaacgaat agaagcagat gacaatgagc gccacgggcg agaggtagaa
     4081 gcagagggaa ttcagcagaa agctggcccc gaccaacccg gcgccggaca agacgcaaag
     4141 ggtcacggca ctgagcaggc tgatctgtcc ggtgggcagg tggcggccgg cggtgcgcgg
     4201 gttcagcgcg tcgaatttgc ggtccacgat gcggttgaag gccatggcac aggtacgggc
     4261 acagaccatc gcaaccagga taagagccaa cgtgcgccaa ccgggccagc cgcggttggc
     4321 gcgggcggca acggccatcg cagccagggc gaagggtagg gcgaagacgg tgtgcgagaa
     4381 gcgcacgaac gagagccatt tattaagtgt cgagaacatc gaacaacatc aaacatcgaa
     4441 caaccaacat ccaatagccc ctcgctagat agacttcctg ggcggatagc gagtggggtc
     4501 cggtgcagcg cgtctttcgg ccagagcatt ccgccgcgcg gtttgaacgc tcgaatagaa
     4561 gatgcggatc aattcgtccg cctcacttaa gagaaagacc agggtcggat catccgtcgc
     4621 ccaggccttg cgcctgatca aacgcgccca tcgacgcgtt tcgcgcagtt ccttgagaca
     4681 gatcttgagc ttgtggatga aatcatctcg cgattcggcg tcctcggctt ccccatgatt
     4741 cgggtagggc gatgtgccgg agcgcagcac ctggccagcg atgtgatttc cggcccgagt
     4801 gtctggcaac ttctccgaca ggtcaatcac cgccgaagca aactccaaca ggcgctcctc
     4861 aaggtcgaat cttcgctgtg ttgtttcgcc ttcccggaat tcccatacct cgctcgcctc
     4921 ctcaatggcc gttggatgtt ggatgttgga tgttggatgt tttggtctct tcatgggttt
     4981 ctttattcct tttccccagc ccatcgcggt gtgagtttgt tcgggatgcc aagatgatcc
     5041 aggacgcgcg ccacgacggt atcgacgacc tgctcgatgg tctggggccg ggtgtagaac
     5101 gaggggttgg ccggcaggat ggtcgcgccg gcctggatga ggagctcgaa gttctttaca
     5161 tggatcaggt ttagcggcgt ttctcggggg acgaggatga gcttcttgcg ctccttcaac
     5221 acgacgtcag ccgcgcgcag cagaacgtct tcactatagc cgtgcgcgat ccggcccagg
     5281 gtgcccatgc tgcacgggat gatgaccatc gcgtcggggg cgctggagcc gctggcgaag
     5341 ggggcgttca tgctcttgag gctgtgcgag acggccccgg caggcaggcg caagccgccc
     5401 ggtagttcct cggcgattac gtccggggcg tagcggctga gcacgacatc tatctggtgc
     5461 tgggcgggat caagattgtc cagcaggcgc tgggcataaa gcgagccgct ggctccggta
     5521 atggcgacga gtattctcaa gggcaacgaa tggttgagtc ttagacggtc ggaatatggc
     5581 gacggcgggc ggattgggca agcgggtttc cggaaccatg cgaaacggct tgtgtaaaac
     5641 gggctcattt gcacaatgct caggtgttaa aaccaggaaa agcttctgcc tgcgtgcccg
     5701 gccttgccgg gggcggcagc gaccgccctg agcttttcac tgcctgcttg ttctgtctgc
     5761 tgttggcggt cgtgctggcg ggctgcgcaa ccggtccgcg cgagaatggg acgcaggcgc
     5821 ctaattggga gagggaggat gtggcgaccg cgtccgtaac cccggttgcc ccggttaccc
     5881 cggttgcgcc gccggctccc gcacccgtgg ccactcctcc gacatcggcc gctcccacga
     5941 accagccagc ggagacctgg attccactcg accgctggtg caaggcgaat ggcctggctg
     6001 cgccgtgttt ggtggggcca gcgccggcgc ccgcctacgc gttgatcatg cctgacggag
     6061 ttgtggtgtt gcgcgcaggc agcctggtgg cgcattggga caggctggac gtccggctgg
     6121 ggttcgcgcc gcagatgatg aacgggcagc ccttcgtcca cgcgctcgac ctgaagaaaa
     6181 ccctggagcc gctggtcagg ggctcgtgcc tgagcttcct caagaccaac ccgatgattg
     6241 tcattgatcc cggccacggc ggccaggatt cagggaccaa gggtgttctg ggctaccgtt
     6301 acgaaaagga cttcacgctc gactgggcgc ggcggctggg ttcgttgctc gccaccaacg
     6361 ggtggcaggt gtttctgacc cgcagtaacg acatggacct gtcggtctcg aaccgcattg
     6421 tctttgccga agcgcacaag gccgatgtat tcctgagtct gcatttcaac tcctccgcgc
     6481 ccaacgttca gcaggcgggc ctggaaacgt attgcctcac gccgaccggc atgccatcca
     6541 ctgtgacccg cgggtacaac gacaaccagg cgctggcctt tccgaacaat gcgttcgatg
     6601 cgcagaacct ccagtttgcc ttgcaggtgc atcgcgcgct gttgcaggtc aatggcgggc
     6661 atgatcgggg cgtgcggcgg gccagatacc tgggtgtgct gcgcggccaa aaccgtcccg
     6721 cgatcctggt cgaaggcggc tacctgtcca atccgagcga agcccgtcgg atcgctaatc
     6781 cggcgtatcg gcaagagctg gcggaagcgg tggcgaaggc gctggccgtg aagtccaacg
     6841 tccaaagtcc aaggtccgaa gtcgcgagcc aggggcggga gcccggaagc cagagggcag
     6901 agaccagaat tcagaaacca gaagccacaa ccacgaacat cgcaacgcct tgagcacgca
     6961 tcacgtttca cgtttcacgt ctcacgttct agtgaccggc ggcgccggct tcatcggatc
     7021 gcacctggta gagcggttgt tggccgacgg caaatccgtc gtcgtcgttg acgatctttc
     7081 caccgggaga ctggagaacc tgcaggcggt gcgcgcgcat cccaagctgc gcgtgattga
     7141 atccaggatt tcgagctgtc cggagttggg ggaactcgtc acccaggcgg aagcgatcta
     7201 tcatctggcg gcggcggtcg gcgtggagtt ggtcgtgcgg tcgccgattc atgtgctgga
     7261 aacgaatttg cacgagacgg aggtgttgct gaaggcggcg gcgggacaag gcgtgccggt
     7321 gctgctgaca tcaacctccg aggtgtatgg caagagccag aaggaggcgt tcggggaaga
     7381 agacaacctg ctgatcggcc cgccgcacca agtgcgctgg agctatgcgt gctccaaatt
     7441 gatggatgag tttctggcga tggcctatgc gaaggagcgg gcgttgccgg tgatcatcac
     7501 gcgcttattc aacacggttg gcccgcggca gacggggcag tacggcatgg tgctgccccg
     7561 gtttatcgcg gcggccagga gcgggcagcc cctgagagta tatggggatg gccggcagac
     7621 gcgctgcttc tgttatgtgc gcgacacggt cgaagcgctg gtgcgcctgc aaggctgcgt
     7681 cgcggcccga ggccaggtgt tcaatgtggg cagcacggag gaaatcagca ttcgagacct
     7741 ggcccgcaag gtaattgaat ccctgagatc ggaatcggcg attgagcttg tcccttacag
     7801 ccaggcttat gagcccggct tcgacgacat gcgccggcgc aagccggtgg tggagaaact
     7861 ggatgggacc gttggctttc gcccgcgcac cggcttgcag accatcatcg agctgaccgc
     7921 cggggccctg acttgaagcg agccctgggg ctgcacttac acgcatcttc gttcataacc
     7981 gagcgagcga gtgagcgcac ggttcgataa cgaacgtggg ctttgcgcgg ggtatggagt
     8041 cccggcttta gccggcaggg tattgccctt caagaccgct cgaaacgctt ggagatccat
     8101 ggcgaaaccg cgtctggccg gctaaagccg gaactccata caccgtaccc ttcgttcgcc
     8161 tgtggaatac acaggctggg acagcttcgg gttggctgcg gacgttacac ttccaggcaa
     8221 caaaaaaggc atcctttcgg atgcctttct gaattgttaa ctcagtccaa ctgagctctc
     8281 caggctttgc ggcctgggta aatcaccgct atctttacgg aaacatcaag cgattgcagc
     8341 caacgggaat agctccagta accgtcccat gatacgaatc acca
//
LOCUS       DATCGS010000306         5078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_76299, whole genome shotgun sequence.
ACCESSION   DATCGS010000306 DATCGS010000000
VERSION     DATCGS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76299"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VIL39_06240"
     CDS             <1..334
                     /locus_tag="VIL39_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481061.1"
                     /translation="APNGVTLTGDKLNDLGAEPRTQAHIYGLAGTAYKIELTGASVAV
                     AAAPESSAADSADAPDGAPQVEVAMPHVFGQAGLILGLALGILALGFAILYRASGTAK
                     ETNERGRG"
     gene            315..1013
                     /gene="ccmA"
                     /locus_tag="VIL39_06245"
     CDS             315..1013
                     /gene="ccmA"
                     /locus_tag="VIL39_06245"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY5481062.1"
                     /translation="MSAVAVEGVWKFYGDFPALRDIRLQADAGACLALIGRNGAGKTT
                     LLRAIAGFSRPGRGRILIEGKEPRDTETRRHLGFIGHGISVYEELSAFENLMLYARLY
                     GLPDPRARALEWLERTGLERVRDGLVREFSRGMRQRLAVARAFLHNPSVLLLDEPFTA
                     LDDRAIAVLQTLLREALAQGKTIIMSTHQLREALELASHVALLTRGKVAFHGPCTAEM
                     TADPGLVYARYGEG"
     gene            953..1693
                     /locus_tag="VIL39_06250"
     CDS             953..1693
                     /locus_tag="VIL39_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY5481063.1"
                     /translation="MYGGNDGRPRAGLRALRRGLMGFFRAALVVMMKDLRAELRTKEA
                     INASLGFAIVILLLFSFAFDPEADTTKALSGGLLWIVFAFAGTLILNRSFARELPNDC
                     LDALIAAPISGAALFLGKALANYVLLLAVELVSLPVFGIFYNVHWTNQLPELLMVMAL
                     GTWALTVIGTVFSALTVNVRLREVMLPMLTYPILLPALMAAMRLTMELISGNAIGPET
                     ATWLKMLIGFDVVYTTVSLFLVQTVLVG"
     gene            1762..2307
                     /locus_tag="VIL39_06255"
     CDS             1762..2307
                     /locus_tag="VIL39_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5481064.1"
                     /translation="MAYYERRLPHWHPDHAAIFVTWRLHGSLPRSQAAQSELPAGKAF
                     VAMDRELAKAATGPCWLKDERVAQCVIATLQFGQEPLRLYGLNTWVLMANHIHILIEP
                     NAALPRITKSIKTFSAKRANEILGRTDDPFWQDESYDHWVRDQNEFGKIAQYIEFNPV
                     AAGLVERPEDWPWSSAYKGAN"
     gene            2391..3065
                     /gene="ccsA"
                     /locus_tag="VIL39_06260"
     CDS             2391..3065
                     /gene="ccsA"
                     /locus_tag="VIL39_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5481065.1"
                     /translation="MREKVLYILGAAALVILAWNTIAISRLPEETQQGVTFKIIFFHV
                     PMWICAMTAACAAFLASVGFLVTKSFKFDAAAVAVTEVGLAFTSAGLVTGSIWGREIW
                     GIWWTWDARLTSALVCWLLYAGYLMLRKAVEDPTQRATFAAVFSIFAFIDVPIVIFSI
                     KWWRTQHPAPVFWAGGSFPPDWSFHFRWNVLAMMMLGVVMVAIRLRQEDAQREIDSLR
                     RTAHAL"
     gene            3076..3282
                     /locus_tag="VIL39_06265"
     CDS             3076..3282
                     /locus_tag="VIL39_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481066.1"
                     /translation="MHALAQVPFLAQLATKEEILQHQYEFLSYGLAAAWVVLAVYVLM
                     MVGRERKLKREIAGLRAMLEEKQK"
     gene            complement(3283..4674)
                     /locus_tag="VIL39_06270"
     CDS             complement(3283..4674)
                     /locus_tag="VIL39_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481067.1"
                     /translation="MVVFLYWPALNSAYRLDDFAWLCLRNTVTAGRSFLWALFSPQAQ
                     GTIRPLGERLWFMLASSLFGLNPIPIHALALCTQIANVLLMADTGWRLLGLRKAAVVA
                     VILWAINDTLVEPLVWASALNEVMYTFWFLAAFNAFLRWTRSRKSAWLWVHAIALALS
                     LGALELAVTFPAIAAVYVLLFEKRRWKGLLPSIAIVVVYVAAHFAAAPLPMDGPYGLN
                     LGWGMAGAFWHYWANVLGPEEYGRIHQTSFALARLGTMLLSAAILLWLGICGRRKRWI
                     PLFCLLWFVLTLAPTLPLLKHVTPYYTFLPAIGLAWLAGDALAGAVSWPGRSLGIACG
                     LLYAVCHIQSTVFVRDWNIDRSRDVVKREARLVDAVREIRRSQPEGPVFLTGLDGEQF
                     WWGLCYGQLTRLGFTDLHVLPDAGDRGIPIPPKEWCRTNDFQFSPGETTRLLREGHAH
                     VFDISHSPPQAAP"
     gene            complement(4916..>5078)
                     /locus_tag="VIL39_06275"
     CDS             complement(4916..>5078)
                     /locus_tag="VIL39_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY5481068.1"
                     /translation="AAEFLIAGATAVQVGTATFWDPGAPVRIAAELAAFLKREKIAGV
                     ALLVGTLEM"
BASE COUNT          997 a         1573 c         1554 g          954 t
ORIGIN      
        1 cgcccccaac ggcgtgacgc taaccggcga caagctcaac gacctcggtg cggagccgcg
       61 aacccaagcc cacatctacg ggctggcagg gacggcctac aagatcgaac tgaccggcgc
      121 gtccgtggca gtggctgcag cgccggaaag ctctgctgcg gactcggccg acgcccctga
      181 cggcgccccc caggtcgaag tggccatgcc gcacgttttc ggccaggccg gactgatcct
      241 gggattggcg ctgggaatcc tggcgctggg cttcgcgatc ctctaccgcg cgtccgggac
      301 ggccaaggag accaatgagc gcggtcgcgg ttgagggcgt ctggaagttc tacggggact
      361 tccccgccct gcgcgatatc cggctccagg ccgatgcggg cgcctgcctg gcgctcatcg
      421 gaagaaacgg cgccggcaag accaccctgc tgcgcgccat cgccggcttc tcgcggccgg
      481 gacgcggcag gattctgatt gagggcaagg aaccgcgcga caccgaaacg cgccgccatc
      541 tcggcttcat cggtcacggc atctcggttt acgaggagct ttcggccttc gagaatctga
      601 tgctctacgc ccggctctac ggtctgcccg atccgcgcgc gcgagccctc gaatggctgg
      661 agcgcaccgg cctggagcgc gtacgggacg ggctggtgcg ggagttttcg cgcggcatgc
      721 gccagcgcct ggcggtggcc cgcgccttcc tgcacaaccc ctcggtgctc ctgctggacg
      781 agcccttcac cgcgctcgac gaccgcgcca tcgcggtgtt gcagacgctg ctccgcgaag
      841 ctctcgccca aggcaagacc attatcatgt cgacccacca gttgcgggaa gccctcgagc
      901 ttgcctcgca cgtcgcgctg ctcacgcgcg gcaaggtggc gtttcacggc ccgtgtacgg
      961 cggaaatgac ggccgacccc gggctggttt acgcgcgcta cggcgagggc tgatggggtt
     1021 cttccgcgcg gcgctggtcg tgatgatgaa agaccttcgc gccgaactgc gcaccaagga
     1081 ggcgatcaac gcgtccttgg gtttcgctat cgtcattctg ctgctgttca gtttcgcctt
     1141 cgacccggag gccgatacca ccaaagccct ttccggcggc ctgttgtgga ttgtcttcgc
     1201 gttcgccggc acgctgattc tgaaccgcag cttcgcccgc gagctgccca acgactgcct
     1261 ggacgctttg atcgccgcgc ccatttcggg ggcggccctg ttcctcggaa aggcgctggc
     1321 gaattatgtt ttgctgctgg ccgtggaact ggtctcgttg ccggttttcg gtatcttcta
     1381 taatgtgcac tggaccaacc aactgccaga gctgctgatg gtgatggcgc tgggtacctg
     1441 ggccctcacg gtgatcggga ccgttttcag cgccctgacc gtgaatgtcc ggctgcgcga
     1501 agtgatgctg ccgatgctga cctatccgat tctgctgccc gcgctgatgg cggcgatgcg
     1561 gttgaccatg gaattgatct cgggaaacgc tatcgggccg gaaacagcca catggctgaa
     1621 aatgctgatc gggttcgacg tggtgtacac gaccgtctct ctgtttttgg tgcagacggt
     1681 gctggtggga tgaggattgc cggagctggt gcttggtggg gcggacctcc tggtctgcct
     1741 tcttggtgtt aatacttcgc tatggcctac tacgaaaggc gcctaccgca ttggcatccc
     1801 gaccacgccg caatatttgt cacttggcgt ttgcacggct cgctgccacg ttcgcaggcc
     1861 gcgcagtccg aattgccagc cggaaaggca ttcgtcgcca tggaccggga attggcgaag
     1921 gccgctacag gtccgtgctg gctcaaggat gagcgcgttg cgcaatgcgt cattgcgacc
     1981 ctgcaatttg gtcaagaacc gctccgtttg tacggcctga atacctgggt actcatggca
     2041 aatcacattc acatattgat cgagccgaat gccgcacttc cccgaatcac gaagagcatc
     2101 aagactttct ctgcgaagcg cgccaatgag attctcggac ggacagacga tcccttctgg
     2161 caggatgagt catatgatca ttgggtgcgg gatcagaacg aatttgggaa gatagcgcaa
     2221 tacatcgaat tcaatccggt tgcggccgga ttagtggaga ggcctgagga ttggccgtgg
     2281 tcgagcgcct acaaaggtgc aaactgaaga aggcagacca ggaggtccgc cccaccggtg
     2341 gggcggacct cctggtctgc cttcttggtt ttaagaagtg gaggcaactg atgcgcgaaa
     2401 aggtccttta catactcggc gcggcggcgc ttgtcatcct ggcgtggaac accatcgcca
     2461 tttcccgctt gcccgaagaa acgcagcagg gggtcacctt caagatcatc ttcttccacg
     2521 tccccatgtg gatctgcgcc atgaccgcgg cctgcgcggc attcctggcg agcgtgggtt
     2581 tcctggtgac gaagagcttc aaattcgacg ccgcggccgt ggctgttaca gaggtggggc
     2641 tggcgttcac gtcggccggc ctggtcaccg gttccatctg gggccgtgaa atctggggca
     2701 tttggtggac ctgggacgcg cgcctgactt ccgcgctggt ctgctggctg ctttacgccg
     2761 gttacctgat gttgcgcaaa gccgtcgagg accccacgca gcgcgccacc ttcgccgcgg
     2821 tgttctcgat tttcgctttc atcgacgtgc ccatcgtgat tttctcgatc aagtggtggc
     2881 gcacgcagca tccggcgccg gtgttctggg ccggcggcag ctttccaccc gactggtcgt
     2941 tccatttccg gtggaatgtt ctggcgatga tgatgctggg cgtcgtcatg gtggccataa
     3001 ggctgcgcca ggaagacgcg caacgcgaaa tcgattccct gcgccggacc gcgcacgccc
     3061 tttaaggaga ttcccatgca tgcacttgct caagttccgt ttctagcgca gctcgccacc
     3121 aaggaggaga tcctccagca ccaatacgag ttcctcagct acggtctcgc cgcggcctgg
     3181 gtcgtcctgg ctgtctacgt cctgatgatg gtcggccgcg agcgcaagtt gaagcgcgaa
     3241 atcgccggcc tgagagccat gctggaagag aaacagaagt aatcatggcg ccgcttgggg
     3301 cggcgaatgg gagatgtcga acacatgggc atgtccctcg cgcagcaagc gcgtcgtttc
     3361 gccgggtgaa aactggaagt cgttggtgcg acaccactcc tttggcggaa ttggaatgcc
     3421 gcgatcgccc gcgtcgggga gaacgtggag atccgtgaaa ccgagcctgg ttaactgccc
     3481 gtagcaaagt ccccaccaga attgctcccc gtccagcccg gtaagaaaca cggggccctc
     3541 tggttgagaa cgcctgattt cgcggaccgc gtctaccagc cgggcctcgc gctttacaac
     3601 atcgcgggac cgatcgatgt tccagtcccg cacgaatact gtggactgga tatggcacac
     3661 ggcatacagt aagccgcatg caatcccgag agacctgccc ggccaggata ccgccccggc
     3721 caaggcgtcg ccggcgagcc aagcgagccc aatcgcggga agaaatgtgt agtaaggcgt
     3781 cacgtgcttc agaagcggta aggtcggcgc cagtgtaagg acaaaccaga ggagacagaa
     3841 gagcgggatc cagcgcttgc gacggccgca aatccccagc cagagaagaa ttgccgcact
     3901 caagagcata gtacccaagc gcgcgagcgc aaaactcgtc tgatgaatcc gcccgtattc
     3961 ctcgggcccg aggacattgg cccaataatg ccagaaggcg ccggccatcc cccatcccaa
     4021 gttcagcccg taaggaccgt ccataggcag cggcgctgcg gcaaagtgcg ccgcgacgta
     4081 gacaacaaca atcgcaatag acggtaacaa ccctttccaa cgacgttttt cgaacagaag
     4141 tacatacacc gcggcaatcg ccggaaaagt aacggccagt tccagcgcgc ccaacgacag
     4201 agcaagcgca atggcatgca cccaaagcca cgccgatttc cgcgaacgag tccatcgcaa
     4261 aaatgcattg aatgccgcca gaaaccaaaa cgtgtacatc acctcgttca atgcggaggc
     4321 ccacacaaga ggttcgacga gagtatcgtt gattgcccag agaatcaccg caaccacggc
     4381 ggccttgcga aggccgagga ggcgccagcc cgtatcggcc atcagcagaa cgttggcgat
     4441 ctgggtacag agcgccaagg cgtgaattgg gattggattc aggccgaaca gagaggatgc
     4501 caacatgaac cacaggcgct cgcccagcgg ccgaattgtc ccttgagcct gcggtgaaaa
     4561 gagggcccaa agaaagctgc gaccggccgt gacggtgttg cgaaggcaca gccaggcaaa
     4621 atcatcgaga cgataggcgg aattgagcgc cggccagtag aggaatacga ccaaggctac
     4681 cgcaagcaaa accccgcccg actgcaatct ttcgttcgca cggcgtgcaa caaacgagac
     4741 gcctgagtgc ggttgtcccc ttggctgaga caaacagtta agcccgcgtt tccattttga
     4801 gtagttaccg ggaatttccg ttttggactc ccggctcccg atcagtatac atccggtcac
     4861 tggccttcgg cgccgcgaat cgcgcctctg tccgccggcc catgcaaact ggcgcttaca
     4921 tctccagcgt ccccaccaac aacgccacgc ccgcgatctt ctcccgtttc agaaacgccg
     4981 ccagttccgc cgcgattctc acgggtgcgc cggggtccca gaacgtggcg gtccccactt
     5041 gcacggccgt ggcgccggcg atcaggaact ccgcggcg
//
LOCUS       DATCGS010000307         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_422969, whole genome shotgun sequence.
ACCESSION   DATCGS010000307 DATCGS010000000
VERSION     DATCGS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422969"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>2360
                     /locus_tag="VIL39_06280"
     CDS             48..>2360
                     /locus_tag="VIL39_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5481069.1"
                     /translation="MPVTEAPDRDLTIPRDSTAAPDAVSAGLPPYPDQFARRHIGPSP
                     QETRDMLHLLGYPSLAALIDDAVPAQIRLRRPLQLPPARSEYEVLAALKEIASQNQVF
                     RSYIGMGYHDCITPPVIQRNLLENPGWYTPYTPYQAEVAQGRLEALLNFQTMVIDLTG
                     LDVANASLLDEATAAAEAMTLCHSLKPGRNVFFVSAECHPQTIDVVKTRAKALDIEVL
                     VGDHQTLQFNDKLCGALVQYPNTLGEVLDYSGFIEQAHAAGVLVAVAADLLSLTVLRP
                     PGEFGADVALGSTQRFGVPLGYGGPHAAYFATRDALKRHIPGRIVGVSKDSRGRPALR
                     LALQTREQHIRREKATSNICTAQALLASLAAMYACYHGPAGLQQIARRIHLHAEVLAQ
                     GLRRLGYEVVSKTFFDTIRVALGDKRAEDILKSAKAARMNFRVIGEHSLGIALDETTT
                     GKDLADILQVFNGNRAPDFNVADLRPQDAAGVRPSSGAAVSPSSASELANVQDESEVT
                     APEDGRTPAPFARTSPYLTHPVFHRYHSETEMMRYLKRLESRDLSLTTSMIPLGSCTM
                     KLSAASEMFPIAWPEFSRLHPFAPLRQARGYQTLFQQLEEWLAEITGFAGISLQPNAG
                     SQGEYAGLLVIRAWHLNRGEGHRNICLIPSSAHGTNPASAARAGLKVVAVACDDQGNI
                     NVADLRAKAEAHKANLAALMVTYPSTHGVFEQPIREICDIIHANGGQVYMDGANLNAQ
                     VGLCRPGDIGADVCHVNLHKTFCIPHGGGGP"
BASE COUNT          429 a          821 c          698 g          412 t
ORIGIN      
        1 gggcggttct cgcttgattt gcaggtaatg gtccttactt tcactggatg cctgttacgg
       61 aagcacccga tagagatctg accatccctc gagactccac ggcggcgccc gacgccgtca
      121 gcgccggttt gcccccatac ccggaccagt tcgcgcgccg acacattggg cccagcccac
      181 aagagacccg ggacatgctg cacctgctcg gctacccgag ccttgcggcg ctgattgacg
      241 acgctgtgcc ggcccagatc cggctgcggc ggccactcca gctcccccct gcccgcagcg
      301 aatacgaagt gctggccgcc ttgaaggaaa tcgcctcgca gaaccaggtc ttccgctcct
      361 atatcggcat gggctaccac gactgcatca cgccgcccgt gatccagcgc aacctcctgg
      421 agaatccggg ctggtacact ccctacacgc cttaccaggc cgaggttgcc cagggccgcc
      481 ttgaagcgct gctcaacttc cagacgatgg tgattgatct gaccgggctc gatgttgcca
      541 acgcctcact gctcgatgaa gccaccgccg ccgcggaagc catgaccctg tgccactcgc
      601 tcaagccagg caggaacgtc ttcttcgtat cggcggagtg ccatccgcaa accatagacg
      661 tggtgaagac ccgggccaag gccctcgaca tcgaagtcct ggtcggcgac catcagaccc
      721 ttcaattcaa cgacaagctc tgcggcgcac tggtccagta tccgaacacc ctcggcgagg
      781 ttctcgacta ctctggtttt atcgagcagg cgcatgcggc gggcgtgctg gtggcggtgg
      841 cggcggacct gctgagcctc accgtgcttc ggccaccggg ggaattcggc gcggacgtgg
      901 ccctcggcag cacgcagcgc tttggcgtgc cgctcggcta cggcggaccg cacgcggcgt
      961 actttgcgac acgggacgcc ctgaaacgcc atatccccgg gcgcatcgta ggcgtgtcga
     1021 aagattcacg cggccgccct gccctgcggc tcgccctcca aacccgggaa cagcatatcc
     1081 gccgcgagaa agccacgagc aacatctgca ccgcccaggc gctgctggcc agcctggcgg
     1141 cgatgtatgc gtgctaccac ggccccgctg gcctccaaca aatcgcgcgc cgcattcact
     1201 tgcatgcgga agttctcgcc caggggctcc gacggctcgg atacgaggtc gtgtcgaaga
     1261 cgttcttcga cacaatccgc gttgccctcg gcgacaaacg ggcggaagat attctgaagt
     1321 ccgcgaaagc ggcgcggatg aacttccgcg tcattggtga gcattcgcta ggcatcgcgc
     1381 tcgacgagac cacgaccggg aaggacttgg ccgacatcct ccaggtgttc aacggcaacc
     1441 gggcgcctga cttcaatgtg gccgatttgc gccctcagga tgccgccgga gtgcgcccgt
     1501 cctcgggcgc agccgtgtcg ccaagcagtg cctcggaact agctaacgtg caggacgagt
     1561 cggaggttac tgcgcccgag gatgggcgca ctccggcccc attcgcgcgc acgagcccgt
     1621 acctgacgca cccggtcttc caccgttatc actccgagac cgagatgatg cgctacctca
     1681 agcgcctgga atcgcgcgat ctctcgctga cgacttccat gatcccgctc ggctcctgca
     1741 ccatgaagtt gagcgcggcc tcggagatgt tcccgatcgc gtggcccgag tttagccgcc
     1801 tgcatccgtt cgcgccgctc cggcaggcgc gcggctatca gaccctattc cagcaattgg
     1861 aggaatggct tgccgaaatc accggttttg ccgggatctc gctccagccc aatgccggct
     1921 cgcagggcga atatgccggc ctcctcgtca ttcgcgcctg gcacctcaat cgtggcgagg
     1981 gtcaccgcaa catctgcctc atacccagct ccgcccacgg caccaatccc gccagcgccg
     2041 ccagggccgg cctgaaagtc gtggccgtcg cctgtgatga ccagggcaac atcaacgtcg
     2101 ccgacctgcg cgccaaagcc gaggcgcaca aagccaacct ggcggcgttg atggtcacct
     2161 atccctccac gcatggcgtg ttcgagcaac cgattcggga gatttgcgac atcatccacg
     2221 ccaacggcgg tcaggtctat atggacggcg cgaacctcaa tgcgcaagtc ggtctgtgcc
     2281 ggcccggaga catcggcgcc gatgtgtgcc atgtgaatct gcacaagacc ttctgtattc
     2341 cgcatggcgg cggggggccc
//
LOCUS       DATCGS010000308         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_621220, whole genome shotgun sequence.
ACCESSION   DATCGS010000308 DATCGS010000000
VERSION     DATCGS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621220"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIL39_06285"
     CDS             complement(<1..224)
                     /locus_tag="VIL39_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481070.1"
                     /translation="MKTRTSRICLFASLGAASLVALSTTAQTAAPSSTAPSIPAATDA
                     APVKLPYGAEDVLKLNRAQVSEDVTLNFIH"
     gene            513..971
                     /locus_tag="VIL39_06290"
     CDS             513..971
                     /locus_tag="VIL39_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481071.1"
                     /translation="MAYSTMANLSPVLLAEDNPNDVELVRRAFQENKTLNPVHDVGNG
                     DEAIQYLAGEGKYADRAAYPFPVLFLLDLKMPVKDGLEVLRWLHEHPDIPCKLPVVVF
                     SSTELPSETQRAYAMDIQACIVKPLSYPELLEKIRILKEYWLDYELEASL"
     gene            complement(975..1898)
                     /locus_tag="VIL39_06295"
     CDS             complement(975..1898)
                     /locus_tag="VIL39_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5481072.1"
                     /translation="MEALTAKTIALADPPEKRKKLPDRPCGCQETAFGAPRDFLDNRY
                     VYAVVSARARGLSVGVNLCPDKICNFQCVYCEVHRNGEPPEGPLDVDAMATELKKTLA
                     FVRGGRLRERPWYRALPDELLQLRHVALSGDGEPTLSPRFAEALQAVVHVRALSGLPF
                     FKLVLLTNATGLDLPQVQQGLKHLTRSDEVWAKLDGGTQAYANKVNRSSVPLEKILSN
                     ILLLGRQRPIVIQSLFPAINGEEPPLEEIEQYARRLLELKHAGAQISLVQIYSATRPS
                     LNSASGHLPLKVLSRITQTVRQATGLTAEVF"
     gene            2058..2915
                     /locus_tag="VIL39_06300"
     CDS             2058..2915
                     /locus_tag="VIL39_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5481073.1"
                     /translation="MGSLLFIQKRLKSFHTDMGSSLAEFRTAAIVNTLRGCQIFAGLA
                     PGDLNAIADITLVKSLGKDDYLFREGAPAQGFYVVQKGAINVHRVNAAGKEQIIHVFR
                     AGESFAEAALATSTGYPADARALESSQVLLVEKAGFLALLRRQPELALRMLGSMSLHL
                     RTLIAQLEDLTLKDVETRLANWLIKRCPAPESAQPVVIELKTTKRVLAAELGTVSETF
                     SRTLATFRQQKLVTVKGKTLTVLCPQKLKALLRRNLGEQTQASRASNRSGSGDLSSVK
                     RHSAKRPCH"
     gene            2972..>3715
                     /locus_tag="VIL39_06305"
     CDS             2972..>3715
                     /locus_tag="VIL39_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5481074.1"
                     /translation="MSRAILLLAGLWLCAAVSGIDLRAATGADSLQRSFDVWQVGEDL
                     EQNPVTAVVQSRDGYLWLGTYTGLLRFDGVRVTMFNSATAPGLLNSRVTSLYEDRDGV
                     LWIGHETGELTRLSGGEFRPMGRAHGWVGGAIEAISMDENGDLWLLNDTGFLFRQRDG
                     HVVEVPGGGAGSRKASLSRERGGKLWITANGNVATLDRGELASFRFDGTNGARFFARV
                     VPARDGGLWVMADGRLRKWREGRWVADLGD"
BASE COUNT          736 a         1056 c         1176 g          747 t
ORIGIN      
        1 ttgtggatga agttcagggt aacgtcttcg ctgacttgtg cgcggttgag cttgaggacg
       61 tcctcagcgc cataaggcag tttgaccggc gcagcatccg ttgcggccgg aattgagggt
      121 gccgtggagg atggcgccgc agtctgtgcc gtggtgctca gggctacgag agaagctgca
      181 cccaggcttg cgaacaggca gattcgagaa gtccgtgttt tcataaaacc atagtctttc
      241 agttctaagt caacttcgcc gggaagggcc gggccgtcaa gccttccaaa gcccctgctg
      301 gcacagagcc ttaatccctg ctcataatac cgttggtact tggacgtggc ggccgggatt
      361 cgtattcgag actccacaag tgagcctgcg cttgcgccca acctctgcgc ccaagccccc
      421 cctgaaccta atagcgatgt ggtcagaatt agccatcctt cgctacctgg atagtgcgtt
      481 ccttgactgc gaatccaaaa taagatattg atatggcata ttctaccatg gcgaatctca
      541 gcccggtcct attagccgaa gataatccga atgacgttga actggtgcgt cgggcattcc
      601 aggaaaacaa aacgcttaat cccgtgcatg atgttgggaa cggcgacgag gcgattcaat
      661 acttggccgg cgaagggaag tatgccgacc gcgccgccta tcccttcccg gtgctgtttc
      721 tgctcgattt gaagatgccc gtgaaggatg ggttggaggt tctccggtgg ctgcatgagc
      781 accccgatat cccatgcaag ttgccggtgg tggtgttcag ctcgaccgag ctgccgagcg
      841 aaacgcagag agcgtacgcg atggacattc aagcgtgcat cgtgaagccc ctgagctatc
      901 ccgagctgct ggagaagatc cggatattga aggagtactg gctggactac gagctcgaag
      961 cgagcctctg aaggctaaaa aacttccgcg gtcaacccgg tcgcctggcg cacggtctgg
     1021 gtgatgcggg agaggacttt cagcggcagg tgtccgctag ctgagtttag tgacggacgc
     1081 gtcgcagaat agatctgcac gagggaaatc tgggcgccgg catgcttgag ttccagcaag
     1141 cggcgagcgt attgctcaat ctcctccagc gggggttcct ctccattgat ggccggaaag
     1201 aggctctgga tcactatcgg gcgctggcgg ccaagcagca ggatgttcga caagatcttc
     1261 tccagcggca ccgatgaccg gttgactttg ttggcatagg cctgggtgcc cccatcgagc
     1321 ttggcccaga cctcgtcgct ccgggtgaga tgtttgagtc cctgctgaac ctggggcagg
     1381 tccagcccgg tcgcatttgt cagcaacacg agcttgaaga aaggaagccc gctcagcgcg
     1441 cgaacgtgca cgaccgcttg cagcgcctcg gcaaacctgg gcgagagggt cggctcaccg
     1501 tcgccgctca aggccacgtg ccgcagttgg agtaattcat ccggcagagc gcgataccag
     1561 gggcgttccc gcaatcggcc gccgcgcacg aatgccagcg tcttcttcaa ctccgtcgcc
     1621 atcgcgtcca cgtccaatgg cccctcggga ggttcgccgt tacggtgcac ttcgcaataa
     1681 acgcattgga aattgcagat cttgtcgggg cacaggttga cgccgaccga caagccacgg
     1741 gcgcgcgccg agaccacggc atagacatag cggttatcca ggaaatcgcg cggcgctccg
     1801 aaagcggttt cctggcagcc gcaaggccga tccggaagct tcttcctctt ttccggtgga
     1861 tcggccaacg caatggtctt tgcagtaagt gcttccacaa tgtctagcgt aacgtagcgc
     1921 tgtcgcccag tggccactac acgatgcttg tcaaagagtg gacatttctt ggcggggatt
     1981 cctcgggcca gctatggtta gtcctgctaa tcccaattaa acaaagctca aatgtcaggg
     2041 gggaacgggc tgcgtgaatg ggcagtttac ttttcattca aaagcggctc aaatccttcc
     2101 ataccgacat gggcagctca cttgcagaat ttaggaccgc cgcgattgtc aacacgctcc
     2161 gcggctgcca gatcttcgcc ggcctcgcgc cgggcgatct gaatgctatc gcggacatca
     2221 ccctggtgaa gtccctcggc aaagacgatt acctctttcg cgaaggcgct cccgcgcagg
     2281 gcttttatgt tgtgcagaaa ggcgcgatca atgttcatcg cgtcaacgcc gccggcaagg
     2341 aacagatcat ccacgtcttt cgcgccggcg agtctttcgc cgaggcggcc ctggcgacct
     2401 cgaccggcta cccggcggat gcgcgcgcgc ttgaatcctc gcaggtgctg ctggtggaaa
     2461 aggcgggatt ccttgccttg ctccggcgcc agcccgagct ggcgctgcgg atgctcggct
     2521 cgatgagcct gcatttgcgc acgctgatcg ctcagctcga ggacctgaca ttgaaagacg
     2581 tggaaacacg cctggcgaac tggctgatca agcgttgccc cgccccggaa tcggcccaac
     2641 cggtcgttat cgagctcaag accaccaagc gcgtgctggc cgccgagctg gggacggtca
     2701 gtgaaacctt ttcacgcacc ctggccacat tccggcaaca gaagctggtc acggtgaaag
     2761 gcaaaaccct caccgtcctc tgtccccaga aactcaaggc gttgctccgc cgcaacctcg
     2821 gggaacaaac gcaagcttcc cgggcatcca ataggtctgg cagcggggat ttgagctcgg
     2881 tgaaacgaca ttcggcgaag cgaccttgcc attgatggga agattcctta gcctgccgca
     2941 catgttcgcg ctaccggcat gccgggattg aatgagtcgt gccattctcc tgctcgcggg
     3001 gttatggttg tgtgcggcgg tcagcggcat tgacctccgg gctgccacgg gcgcggacag
     3061 cctgcagcgc tctttcgatg tctggcaggt gggggaggac ctggaacaaa acccggtcac
     3121 cgcggtggtg caatcgcgcg atgggtattt gtggctgggg acctacaccg gcctgctccg
     3181 tttcgatggc gtgcgcgtga cgatgttcaa ctcggccacc gcgccggggc tgctgaacag
     3241 ccgggttacc agcttgtacg aggaccgcga cggggtgctc tggattggcc atgaaacagg
     3301 cgaactcaca cggttgagcg gcggggaatt tcggccaatg ggccgggcgc atggttgggt
     3361 gggaggggcg atcgaggcca tcagcatgga cgagaacggg gatttatggt tgctgaacga
     3421 caccgggttc ctgttccggc agcgcgacgg gcatgtggtt gaagttcccg gcggcggtgc
     3481 ggggagccgc aaggcatcat tgagccgaga acgcggcggc aagttgtgga ttacggcaaa
     3541 cggcaacgtg gcaacccttg accggggcga gcttgcctcc ttcaggttcg atggcaccaa
     3601 tggggctagg ttcttcgcgc gagtcgttcc ggcgcgggat ggggggttgt gggtgatggc
     3661 ggatggccgg ctgagaaaat ggcgggaagg tcgctgggtg gcagacctgg gcgat
//
LOCUS       DATCGS010000309         3029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_274312, whole genome shotgun sequence.
ACCESSION   DATCGS010000309 DATCGS010000000
VERSION     DATCGS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274312"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..808
                     /locus_tag="VIL39_06310"
     CDS             38..808
                     /locus_tag="VIL39_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY5481075.1"
                     /translation="MSLLAGKLGIVFGVANRRSIAWAIAQAWHRAGAKLAFTYQGQRL
                     KENVEELAGAFGADTLILPCDVTKDDEIAGIFKVVGDKFGKLHLLLHSVAFAPKDALE
                     GEFVNTSREAFRIAHDVSAYSLVALARAAAPLMPEGGSIVAMSYYGAEKVVPHYNVMG
                     VAKASLEASTRYLAYDLGPKKIRVNCISAGPVNTLAARGIGGFNEMLKQYEARAPLKR
                     NVLPEELGATGLFLASDGAAAITGQVLYVDCGYQIMGM"
     gene            805..939
                     /locus_tag="VIL39_06315"
     CDS             805..939
                     /locus_tag="VIL39_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481076.1"
                     /translation="MNRRQRFPEGKWVPLDEFLAKKLNVRISHGISPEAFEEIKKSLE
                     "
     gene            complement(955..2283)
                     /locus_tag="VIL39_06320"
     CDS             complement(955..2283)
                     /locus_tag="VIL39_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481077.1"
                     /translation="MKRRQFLKSVAFSGAAGLILPEIRLFGADAPSNKLNIALIGTWG
                     RGEAHFGGMATENVVALCDINEDHLNFGARKFPKAQKYVDWRKCLEQKDIDAVICCTA
                     DHTHAFVANWSMNRGKHIYCEKPLGNCVNEVRVVRANYLKNKNKLATQVGTQRHQYEN
                     FNRVRELILDGAIGKLEEVSAWGNRKIPKPGYLPAKGTPPEGFHWDLWLGPSPEHPYN
                     PEYFSGGSGANCLQWNMYWDFGNGQIGDMGSHTMDLAWNPLDAELPTTAEAKGDPFNP
                     EVTPVKLEMHFDIPANNWRSGIKASWYQGGAMPENPPGSSVDLRRIDHGALFEGSRGS
                     IVAGFESRMILPGRGADMTYYKPRTKENLLPPVGDFQRQWINACKGNGKTCCDFDYGG
                     KMIESMLLGLVAYRVGKKIKYDGAAGKVTDCPEANELLSRKYRAGWTLDG"
     gene            complement(2599..>3029)
                     /locus_tag="VIL39_06325"
     CDS             complement(2599..>3029)
                     /locus_tag="VIL39_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEY5481078.1"
                     /translation="TIPDTVTSIGDYTFQGCTSLGGVYFQGNAPSAGSFMFVGAGNLT
                     VYYLPGTTGWGSPFDGITAVVWMPQIQTSDGSFGVRMNQFGFTITWASDLVVVVEACT
                     NLANPLWSPVGTNTLTNGSSYFSDPQWTNYPSRLYRLRSP"
BASE COUNT          568 a          934 c          923 g          604 t
ORIGIN      
        1 tttggtgcct taatcaattg acgcatttag ggaacgcatg tctttactgg ctggcaaact
       61 tggaatcgtc tttggcgtgg ctaaccgccg ctcgattgcg tgggccatcg cccaggcctg
      121 gcaccgcgcg ggcgcgaaac tcgccttcac ctaccagggc caacggctca aggaaaacgt
      181 cgaggaactg gccggcgcat tcggcgccga caccctgatc ctgccgtgcg acgtcaccaa
      241 agatgacgaa atcgccggca tcttcaaggt ggtcggggac aaattcggca agttgcacct
      301 cctgctgcac tcggtggcgt ttgcgcccaa ggacgccctc gaaggcgaat tcgtcaacac
      361 cagccgcgag gcatttcgca tcgcccacga cgtcagcgcc tattcgctgg tagcgctggc
      421 gcgcgccgcc gcgccgctca tgccggaggg cggcagcatc gtcgccatga gctactacgg
      481 cgcggagaag gtcgtgccgc actacaacgt gatgggcgtg gccaaggcct ccctcgaagc
      541 cagcacgcgc tatctcgcct acgatctcgg cccgaagaaa atccgcgtca actgcatcag
      601 cgccggcccg gtgaacacgc tggcggcccg cggcattggc ggcttcaacg agatgctgaa
      661 gcaatacgaa gcccgcgcgc cgctcaagcg caacgtcctg cccgaagaac ttggcgccac
      721 cggcctgttc ctcgccagcg acggcgcggc agccattacg gggcaggtcc tttacgtgga
      781 ctgcgggtat cagatcatgg ggatgtgaac cgcagacaac ggtttccaga aggcaagtgg
      841 gttccgctgg atgagttcct cgcgaagaag ctcaacgtgc gaatatccca cggcatctcg
      901 cccgaagcct tcgaggagat caagaagtcg ttggagtgaa gccggttcgg ccgatcagcc
      961 gtcgagggtc cagccagcgc gatacttgcg gctcagcaat tcattcgcct cggggcagtc
     1021 agtcaccttc cccgccgcgc catcgtactt gatcttcttg ccgacgcggt aggccaccag
     1081 gcccagcagc atcgactcga tcatcttgcc gccgtaatcg aagtcgcagc aggtctttcc
     1141 attgcctttg caggcgttga tccactggcg ctggaaatcc ccgacgggcg ggagcaggtt
     1201 ctccttcgtg cgcggcttgt agtaggtcat gtccgctcct cggcccggca gaatcatgcg
     1261 ggactcgaaa ccggcgacga tgctgccccg gctgccttca aagagcgcgc cgtggtcgat
     1321 ccgtctcagg tccacgcttg accccggcgg gttttcaggc atggcgccgc cctggtacca
     1381 ggaggccttt atccctgacc tccagttgtt ggcgggaata tcaaaatgca tttccagctt
     1441 aacgggggtc acctcgggat taaagggatc gcccttggcc tcggcggtgg tggggagctc
     1501 cgcatcgagc gggttccagg ccaggtccat cgtgtgactg cccatatcgc caatctgccc
     1561 gttgccgaag tcccagtaca tattccattg gaggcagttt gccccggagc cgccggagaa
     1621 atattccggg ttgtaggggt gctcgggaga gggtcccagc cacaggtccc agtggaagcc
     1681 ttccgggggg gtgcctttcg cgggcaggta gcccggcttc gggatcttgc ggttgcccca
     1741 agcgctcact tcctccagtt tcccgatcgc cccgtcgagg atcaactccc gcacgcggtt
     1801 aaagttctcg tactggtggc gttgcgtgcc gacctgcgtg gccagcttgt tcttgttctt
     1861 gagatagttc gcccgcacca cccggacttc gttcacgcag ttgccgagcg gcttctcgca
     1921 gtaaatgtgc ttgccacggt tcatggacca gttggccacg aaggcgtgcg tgtgatccgc
     1981 cgtgcagcag atcacagcgt cgatgtcttt ttgttcgagg catttcctcc agtccacata
     2041 cttctgggcc ttggggaatt tccgcgcccc gaagttcagg tgatcctcgt tgatgtcgca
     2101 gagagccacc acattctcgg tggccatccc gccgaaatgg gcctcacctc ggccccaagt
     2161 gccgatgagg gcaatgttga gtttgttgct gggggcgtcc gctccgaaga gccgaatttc
     2221 cgggaggatg aggccggccg ccccgctgaa ggccacgctt ttaagaaact ggcgtcgttt
     2281 catttggcga tgattaatca catctcccag gatgtcaaga acacgcatat gcattacccg
     2341 ttaccacgga gcgagggaga ggccgcccaa accaaccgcc tttcaatgac cgatcatcaa
     2401 ttggcggaga cgggtcttcc tccgcactgc aatagccgaa gttggtcgca agctgcgaag
     2461 actcaacgcc gaggcgcaaa gattcgcgga gattcgcaga gagttctctt tctttgcgtt
     2521 aagacgggtt ttgctttggc tgcggcttgg ccgcgcagcg cccatcgcgg ctccgtggtt
     2581 tcaccggact cccgcgccct acggtgagcg caggcggtag aggcgggagg gataattcgt
     2641 ccactgggga tcgctgaaat aggacgagcc attggtgagg gtgttcgtac ccaccggaga
     2701 ccacagggga ttagccaggt tcgtgcaggc ttccaccaca acgaccaagt cgctggccca
     2761 ggtgatggtg aacccaaatt gattcatccg cacgccgaag ctgccatcgc tggtctggat
     2821 ctgcggcatc cacaccacgg ccgttatacc gtcaaatggc gagccccagc cggtagttcc
     2881 cggcaggtaa tagacggtca gattgccggc cccgacgaac ataaacgagc cggcgctggg
     2941 ggcgttgccc tggaaataga ccccgcccag gctggtgcag ccctggaacg tatagtcgcc
     3001 gatgctggtg acggtgtcgg ggatcgtga
//
LOCUS       DATCGS010000310         6325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_473226, whole genome shotgun sequence.
ACCESSION   DATCGS010000310 DATCGS010000000
VERSION     DATCGS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473226"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VIL39_06330"
     CDS             complement(<1..659)
                     /locus_tag="VIL39_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481079.1"
                     /translation="MKTTLLTLFLTGFGLAAQIPSFPAPRTNAPPDAATRASTLTNRQ
                     EMLRQELRRTLAGGTNAAALGTNVNPVKAPVGMDSSAPAAAGRNTPSPLSAAKPDGLT
                     TPPGVPPAGPGPAASAPPIGAVAPPPGATISSLEEPLKEGIIDFRSADLNQVLEVYSI
                     MVNRTILRPSTLPAAPITLTTHGQLTVREGIMALEAVLGLNGITMINMGDKFVKAMPE
                     A"
     gene            complement(662..1273)
                     /locus_tag="VIL39_06335"
     CDS             complement(662..1273)
                     /locus_tag="VIL39_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481080.1"
                     /translation="MTNPLDRLNLRPFEKRLVVAVAVVLFVVLNAWFVFPHFSDLGQA
                     QKQREDALKKIERWNAEIHQASGYQRQIKELESEGQSVPAEEQQNQFSRAIQMQQAQS
                     GVNITSTSKQIVRTNQFFLELTQQISVESGEAQLVDFLYNLGSGSSLIRVRDLGVHPN
                     PPRQALSGTVKLVASFQKNPPKKTAPAAKPSGATPRTVTSTAK"
     gene            complement(1270..3330)
                     /locus_tag="VIL39_06340"
     CDS             complement(1270..3330)
                     /locus_tag="VIL39_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481081.1"
                     /translation="MDYKRTECWSAGVVEYCNIPTPRPSITPLLHYSITPRFIRCLTL
                     RLAGRLLIGAALLAMTMTGCGPSSNKAGKQSGTNAAAPRVLSKTNPAPALRPGVITNL
                     VPATSLAAAKANAAAVAAARAAGKAKAAQAAARPVAKTNAPPITARTGASTNAASAAG
                     AKSGIAEKVRSLQTNPAFYPAVGVVFVCLCLAVVLVVRLLKSKAAKASQVAQPGTAVV
                     GAAKPARKKAGIVAIHSCNVLQVGAQSRQLWQFDARGKAFVLNREQTSFAGEPLPARS
                     VTKDWRSLWQHKLNIAWLPPERVFLRVAQFPQSDLDDTLAMVELQLEKLSPMPVTQIV
                     WSIQVLPHAEGNLQTVIVMIVSRNEVEEFLGQLEGQGYLADRLELPLLDQLQATTITQ
                     DGAWIYPEPPGGRNAALVAWWYGGVLQNLDLITLPPANRPESLKEQLLQMAWAGELEG
                     WLTAPPVWHLVADPPAVAEWEPALRSGLEQPVEVIAPVPTAGLAALTAARAAQAGPQA
                     NLLPAEFSTRYHQQFVDRLWMRGLGAVVALYLVGVVVYFARLQWELYRTNAVVQQVAE
                     LGPTYTNSIQLRDRFKILKDRAELKYAALDCWKTVAELLPDGVTLDGYNFSDGKKLTL
                     SGSAPADQIKRLLDFDADIRKAAVNGQPLFDPNAGDHVTYRSGPGGAVSWSCVLELKR
                     SEAL"
     gene            complement(3370..5121)
                     /locus_tag="VIL39_06345"
     CDS             complement(3370..5121)
                     /locus_tag="VIL39_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5481082.1"
                     /translation="MAFLSLKQFLARMGLATFEQFDEWNKAWKVAIASGSQETLLAFI
                     CRERGLAEDVFLPQLAQALTWPYLDLPKLSVPAEARNRISTKVAFQYSVLPTAVTDGT
                     LQVVVSNPFDTAMLNAVRFDARGPVGFALATKVEIEKALKKYYGVGAETLDEMAEDEP
                     LELLVGEDKEITESDQEASVIKFVNQVIWEAFKDRSTDIHFEPAEDELRIRYRIDGIL
                     HQTPMPPQLKRYQAALISRIKVMSGMNISEKRLPQDGRINVRIKGEEIDIRVSTVPTV
                     YGESVSLRLLTRGKIFLSLDKLGFSPLEEKSIREIIVKPHGIFLVTGPTGSGKSTSLY
                     AFLSTINSVHKRIITIEEPVEYELKGINQIAVRPEIGLTFAMGLRHILRQDPNVIMIG
                     EIRDLETAEIAIRAALTGHLVFSTLHTNDAPSAFTRLIDMGIEPFLIASSVESVMAQR
                     LVRTICPHCKQEQKVERDYLRRISFPEDEIETAKFWHGVGCEQCRQLGYQGRMGIYEL
                     LVLNESLRPLILSRAPASTLAQRAIEFGMRTLRIDGWNKVRAAQTTIEEVLRVTQIEE
                     HLDSLGEGAKGTALAKK"
     gene            5216..6064
                     /locus_tag="VIL39_06350"
     CDS             5216..6064
                     /locus_tag="VIL39_06350"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY5481083.1"
                     /translation="MKQFATITVIGRDKTGVVARITNFLFLQKANIEALEEQVTRGQF
                     SMTVQTSWKARDLDRDGVRRGLEKQASELDMEVKIRFTEPRRRQRMGLMVTREPHCFA
                     KILGAIKSGGLKKADPALVLSNRRDLEPLARENRLPFVHIPWEDRLRAEQQALRVLDE
                     HEIDFIVLARFMKILSPNFVWRFKNKIINIHPSLLPSFPGPQAYRQAYEQGVKIIGVS
                     SHFVTMNLDEGPIIAQDCFAVRPQMTLKQIVAAGQKIEAATLLKAVKLYLQKRLDVYW
                     GIVKEV"
     gene            6091..6228
                     /locus_tag="VIL39_06355"
     CDS             6091..6228
                     /locus_tag="VIL39_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481084.1"
                     /translation="MRDKKNMMRALIPVTPHASRITHHASRITHHASRFTLAFARAML
                     A"
BASE COUNT         1172 a         1981 c         1938 g         1234 t
ORIGIN      
        1 tgcgcctcgg gcatcgcctt aacgaacttg tcccccatgt tgatcatcgt gatgccattg
       61 agccccagca ccgcttccag ggccataatc ccctcccgca cggtaagctg cccgtgcgtc
      121 gtcagggtga tgggcgccgc cggcaaagtc gagggccgca agatggtccg gttgaccatg
      181 atgctgtaca cctccagcac ttggttcagg tcggcgctcc gaaaatcgat tataccctcc
      241 ttcagtggtt cctccaacga ggagatggtg gctcccggcg gaggagcaac ggcgccgatg
      301 ggcggggcgg acgccgccgg cccgggcccc gcgggtggaa cccccggggg ggtagtaagg
      361 ccatcaggct tggccgctga cagcggagat ggcgtgttcc gcccggcagc cgcaggcgcg
      421 gacgagtcca tgccaacagg agctttgacc gggttcacgt tggtcccaag ggccgccgca
      481 ttggtgccgc ctgcgagggt gcgacgcagt tcctgcctga gcatttcttg acgatttgtc
      541 agcgtggaag cgcgggtggc ggcatccggc ggggcattcg tgcggggcgc gggaaaggag
      601 gggatttgag ccgccaagcc gaagccggtc aggaatagag tgaggagtgt cgttttcacg
      661 gtcatttggc agttgaggtt accgtcctgg gagtggcgcc tgacggtttt gcggctggcg
      721 ccgtcttctt gggcggattc ttttgaaaac tggctaccag tttcacggtg ccgctaagcg
      781 cctgccgcgg cgggtttgga tgcaccccca gatcgcgcac gcgaatcagc gagctgccgg
      841 agccaagatt gtaaaggaaa tccacaagct gtgcttctcc agattcaaca ctgatttgct
      901 gggttagttc caagaaaaac tggttggtgc gaacgatctg cttcgaggtg ctggtaatat
      961 ttacgccgct ctgcgcctgc tgcatctgga tggcgcgcga aaactggttc tgctgctcct
     1021 ccgccggcac ggactggcct tcgctctcca gttctttgat ctgacgctga taacccgacg
     1081 cctgatggat ttccgcattc cagcgttcga tcttctttag cgcgtcctcc cgctgctttt
     1141 gcgcctggcc caaatccgag aaatgcggaa agacaaacca ggcgttgaga acgacaaaca
     1201 acaccaccgc gaccgcgacg accagccgct tttcgaacgg gcgcaagttc aagcgatcca
     1261 ggggattcgt catagtgcct ccgaacgttt gagctccagg acgcaactcc aactcacggc
     1321 cccgcccgga cccgaccggt aggtgacatg gtcgcccgcg ttgggatcga acaagggctg
     1381 accgttgacg gcagccttcc gaatgtccgc atcgaagtcc agcaaccgct taatctggtc
     1441 ggccggcgcg ctgccgctga gggtgagttt cttcccatcg ctgaaattat agccgtcgag
     1501 cgtgacgcca tccggcagca actcggcgac ggtcttccag caatccagcg cggcgtattt
     1561 cagctccgcc cggtccttga ggatcttgaa acggtcgcgg agctggatgg agttcgtgta
     1621 ggtggggcca agctcggcca cttgctgcac cacggcattg gtgcgataga gctcccactg
     1681 gagccgggcg aaatagacca ccacgcccac cagatagagg gccacgacag cccccagccc
     1741 gcgcatccag agccggtcca cgaattgctg atggtaacgg gtcgaaaact cggccggcag
     1801 caggttcgcc tgcggccccg cttgggccgc gcgggcagcc gtcaaggccg ctaatcccgc
     1861 tgtcgggacg ggcgcgatga cttcgacggg ttgttccaat ccggagcgca gggccggctc
     1921 ccattcggcg accgccggcg gatcggctac cagatgccag acgggcggag cggtcagcca
     1981 gccctccagt tcacccgccc aggccatctg cagcaactgc tccttcaggc tctccgggcg
     2041 attggccggc ggcagcgtaa tcagatcaag gttctgcagc acgccgccgt accaccacgc
     2101 gaccagcgcc gcatttctcc cgcccggggg ctctggataa atccaggcgc cgtcctgggt
     2161 gatggtcgtg gcctgcaact ggtccagcag cggcagttcg agccggtcgg ccaggtaacc
     2221 ctgcccctcg agctggccaa gaaactcctc cacctcattc cgcgaaacga tcatcacgat
     2281 cacggtctgc aggttgccct cggcatgcgg caagacctgg atgctccaga caatctgggt
     2341 gacgggcatc ggcgagagtt tctccaactg cagctccacc attgcgaggg tatcgtcgag
     2401 gtcgctctgc gggaattgcg ctacgcgcag gaaaacccgc tccggcggca gccaggcaat
     2461 gttgagcttg tgctgccaca gcgagcgcca atccttggtg acgcttctgg ccggcaaggg
     2521 ctcccccgca aagctggttt gctcgcggtt caggacgaac gccttgcccc gggcgtcgaa
     2581 ttgccaaagc tgccgcgact gggcgccgac ctgaagcacg ttgcaggaat ggatggcgac
     2641 tattcccgcc tttttcctgg ccggcttggc cgcccccacc accgccgtcc cgggttgcgc
     2701 cacctggcta gccttggcgg ccttggactt caacagcctg accaccagca ccacggccag
     2761 gcacaggcag acgaagacga cgcctactgc ggggtaaaac gcggggttgg tttgcagact
     2821 gcgaaccttc tccgcaatgc ctgacttcgc acctgcggct gaggccgcgt ttgtgctcgc
     2881 gccagtccgc gcggtgattg gcggggcatt ggtcttggcg acgggtcgcg ctgcggcctg
     2941 tgccgccttc gccttgccgg cggctcgcgc tgcggcgacc gcggcggcgt tcgccttggc
     3001 cgcggccagc gaggtggccg gaacgaggtt cgtaatgacg ccgggccgca atgccggggc
     3061 tgggttcgtc tttgacaaaa ctcgcggggc agccgcattg gtccctgatt gcttgccagc
     3121 cttgtttgac gacgggccgc agccggtcat ggtcatggcc agcagcgcgg ctccgataag
     3181 cagccgcccg gccaggcgca gcgtgaggca gcgaatgaaa cgcggagtaa tggagtaatg
     3241 gagtaatgga gtaatggagg gtcgaggggt tgggatgttg caatactcca ccactccagc
     3301 actccagcac tccgttcttt tgtagtccat cacgccatga ctccgttcag tcttcactcg
     3361 gcttcgtcct cacttcttcg ccaaggctgt ccccttggca ccctcgccca gcgagtcgag
     3421 gtgttcctcg atctgcgtga cgcgcagcac ttcttcaatt gtggtctgcg cagcccggac
     3481 cttgttccag ccgtcaatac gcaacgtgcg catgccgaac tcgatggccc tttgggcaag
     3541 ggtcgatgcc ggcgcgcggc tcaaaatcag cgggcgcaac gattcgttca ggaccagcaa
     3601 ttcataaatg cccatgcgcc cctggtaacc cagttgccgg cactgctcgc acccgacgcc
     3661 gtgccagaac ttggccgtct cgatttcgtc ctccgggaag ctgatccggc gcaggtaatc
     3721 gcgttccacc ttctgctcct gcttgcaatg cggacagatg gtgcggacca ggcgctgggc
     3781 catcaccgat tcaaccgagg aggcgatgag gaagggctcg atgcccatgt cgatgaggcg
     3841 ggtgaaggcg ctcggcgcgt cgttcgtgtg cagggtgctg aagaccaaat ggccggtcag
     3901 cgccgcgcga atggcgatct cagccgtctc caggtcgcgg atttcgccga tcatgatcac
     3961 attcgggtcc tggcgcagga tatggcgcag ccccatggcg aaggtgaggc caatctccgg
     4021 acgcacggcg atctggttga tgcccttgag ttcgtattcg acgggttcct cgatcgtgat
     4081 aatgcgcttg tgcaccgaat tgatcgtgct gaggaacgcg tagagcgaag tcgatttgcc
     4141 cgacccggtc ggaccggtga cgaggaagat gccgtgcggc ttgacgatga tctcacggat
     4201 ggacttctct tccaacggcg aaaagccgag cttgtcgagg ctcaggaaga tcttgccgcg
     4261 cgtcaggagc cgcagggaga cgctctcgcc atagaccgtg ggcaccgtgg acacgcggat
     4321 gtcaatttcc tcacccttga tccgcacatt aatgcggccg tcctgcggca ggcgcttctc
     4381 ggagatgttc atccccgaca tgaccttgat gcgggagatc agcgcggcct ggtagcgttt
     4441 gagctgcggc ggcatgggcg tctggtgcag gatgccgtca atgcggtagc ggatgcgcag
     4501 ctcgtcttcc gccggctcga agtgaatgtc cgtcgagcgg tctttgaagg cctcccaaat
     4561 gacctggttg acgaacttga tgacgctcgc ctcctggtcg ctttcggtga tttccttgtc
     4621 ctcgcccacc agcaattcga gcggctcgtc ttcggccatc tcgtcgagcg tttcggcgcc
     4681 gacgccgtaa tacttcttga gcgccttctc gatttccacc ttggtggcca gcgcaaatcc
     4741 tactgggccg cgcgcgtcaa accgcacggc attcagcatc gccgtgtcga acgggttgct
     4801 caccaccacc tggagcgtgc cgtcagtcac cgcggtgggt agcaccgagt attggaaggc
     4861 aaccttggtc gaaatccggt ttcgcgcctc ggcgggcacg cttaacttgg gcaaatcgag
     4921 atagggccag gtgagcgcct gggccaactg cgggaggaag acgtcctcag ccaggccgcg
     4981 ttcccggcat ataaaggcca gcagcgtctc ttgcgagccg ctggctatag ccaccttcca
     5041 ggccttgttc cactcgtcga actgttcgaa agtggccagg cccatccggg ctagaaactg
     5101 cttgagcgat aaaaaagcca tctgtcactg ggcaaactgc cccatcatca tgcttctgat
     5161 acacagcaaa ctaagaaaag ttgcgggtgg ggtcaaagta attagactga ctcctatgaa
     5221 acaattcgcc accatcaccg ttatcgggcg cgacaagacg ggcgtggtcg cccgcatcac
     5281 caacttcctc tttctccaga aggcgaatat cgaggccctg gaagagcaag tcacccgcgg
     5341 ccagttcagc atgaccgtcc agacttcctg gaaagcgcgc gacctcgacc gggacggcgt
     5401 ccgccgcggc ctggagaagc aggcgtcaga gctggatatg gaggtcaaaa tccgctttac
     5461 ggaaccgcgc cgacgccagc gcatgggcct catggtcacc cgcgagccac actgctttgc
     5521 gaagatcctc ggcgccatca agtcaggcgg gctgaagaaa gcggacccag ccctggtgtt
     5581 gtccaatcgc cgggacctgg aaccactggc gcgagagaat cgcctgccct tcgttcatat
     5641 cccctgggag gaccggctgc gcgcggagca gcaggcgttg cgagtactcg acgagcacga
     5701 gattgatttc atcgtcctgg cgcgtttcat gaagattctc tcccccaact tcgtctggcg
     5761 cttcaagaat aagatcatca atatccaccc ctcgctgctg cccagtttcc cagggcccca
     5821 ggcctaccgc caggcctatg agcagggggt caaaatcatt ggggtctcct cccattttgt
     5881 gacgatgaac ctcgatgaag ggccgatcat cgcccaggat tgctttgcgg tgcgccccca
     5941 gatgactctt aaacagatcg tcgccgccgg ccagaaaatc gaagccgcca ccttgctcaa
     6001 ggcggtgaag ctttatctcc agaagcggct ggacgtctat tgggggatcg tcaaggaagt
     6061 gtgaggctga tacgtgatcc gggatgcgtg atgcgtgata agaaaaacat gatgcgcgcg
     6121 ctgattcccg ttacccctca cgcatcacgc atcacgcatc acgcatcacg catcacgcat
     6181 cacgcatcac gtttcacttt ggcctttgcc cgtgccatgc tagcttgacg ccgtgagaag
     6241 attcttactc ggcttgctcg gcgccctcgt ggccgccagc ccgctctcgg cggccaccaa
     6301 tctcgtcgcc ggcagcatga acccg
//
LOCUS       DATCGS010000311         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_126857, whole genome shotgun sequence.
ACCESSION   DATCGS010000311 DATCGS010000000
VERSION     DATCGS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126857"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VIL39_06360"
     CDS             <1..267
                     /locus_tag="VIL39_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5481085.1"
                     /translation="LRDGLLQKVNLIVATGQNNLAMNQTVAQIARHYVRGDKIPEAML
                     NRVEHGIRCYDPCLSCSTHAVGQMPLHVQLIAPDGTVLNEIARG"
     gene            456..923
                     /locus_tag="VIL39_06365"
     CDS             456..923
                     /locus_tag="VIL39_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY5481086.1"
                     /translation="MTPEQPQSPTGLLVIGYGNTLRSDDGVGPRVADAVAALALPGVR
                     ALVCPLLTPELADPVSQARVVIFVDAAVDAPREVQMRKLAAAGTSQIMAHAASPATLL
                     ALARDVFGHVPEAWWLTIPAEDLGIGEELSPLAQRGFEIAVQEVRKCAANLGL"
     gene            complement(1029..2408)
                     /locus_tag="VIL39_06370"
     CDS             complement(1029..2408)
                     /locus_tag="VIL39_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481087.1"
                     /translation="MNTRTPKQSLATLCTLSLLCGFAGLALAQDKAPAGPRHPPFHFA
                     DYDSEPRRADGHVDGDVLLTRLKELHVTTYYWLVWHARTDWDDLKLFLPKAAQANIQV
                     WVYLVPPTESAPREGDLYPEPFRLDYGRWAEEIARLSLQHTNLTAWVIDDFYANHKFF
                     TPATVRDFQKRAKSINPRLVFLPLMYFGEIRRAFVQDYRDVIDGVVVAYPQDRDEVDQ
                     AWAILNDAAVTMSGELSYPASTPSQPGDFVRASQSATAVSSGRQRLRFRERDDFTAGT
                     SGYHFKQLLVNDTVVWEADVAGGTNAWGEVAVDVTAAAQGKTNLTVVFRLLDKQGVSN
                     FGVRWQVKDLQAEGLTLAAGLDQPQRWQVSQRGAFEAGFGDRIKTPEHRFHVPFIVMT
                     AGDASEFKLRHGDPASPERIAEWLKFCLQAWREGKCDGVVTYCLDKQPRSRIFDLARD
                     LFGQYRQPQ"
     gene            complement(2505..>2742)
                     /locus_tag="VIL39_06375"
     CDS             complement(2505..>2742)
                     /locus_tag="VIL39_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481088.1"
                     /translation="HSGVFAFQAAPAPSDATEHAQRLGLRWGIVHSHYLTPAKVEFTT
                     YDGRTVAVAKVSADQIRSLGSKEIHHARALTAET"
BASE COUNT          507 a          847 c          874 g          514 t
ORIGIN      
        1 ctgcgcgacg gcctgttgca aaaggtcaat ctcatcgtcg ccaccggcca gaacaatctg
       61 gccatgaacc agaccgtcgc gcagatcgcg cggcactatg tgcgcggcga taaaattccc
      121 gaagccatgc tcaaccgcgt cgagcacggc atccgctgct acgacccgtg cctgagctgc
      181 tccacgcatg ccgtggggca aatgcccttg cacgttcagc tcatcgcgcc ggacggcacg
      241 gtgctgaacg aaatcgcgcg cggttgaccc cgcggagaga caagtccggt gggcgcatcc
      301 gcgctgtgcc accgtagcgc agatttgtaa tctgccatat cgcggatttg caatccgccg
      361 ggtctggcaa gcttgcttcg catgggcagt aagggcgcgc tgccgaatac aattcggcga
      421 tacagcagat tacaaatctg cgttacgcta aacggatgac accagaacaa cctcagtcac
      481 cgaccggcct cctggtcatt ggctacggca acacgttgcg cagcgatgac ggcgtgggac
      541 ccagggtggc cgatgcggta gcggcgctcg ccctgcccgg cgtccgcgcg ctggtctgcc
      601 cgctcctgac ccccgaactc gccgacccgg tttcccaggc gcgcgtcgtt atctttgtgg
      661 atgctgcggt ggacgcgccg cgagaagtcc aaatgcgcaa attggcggcc gccggcacct
      721 cccagatcat ggctcacgcg gccagtccgg cgaccctgct ggccctggcc cgtgacgtat
      781 ttggccatgt ccccgaggct tggtggctca cgattcccgc cgaggacctc ggcatagggg
      841 aggagctttc accgctagct caacgcggat tcgagatcgc ggtgcaggaa gttaggaagt
      901 gcgctgctaa cttgggcctt tgagctttga gaagccttcg ccctgcgctc tcattaacac
      961 ccgctgaagc ttgatggcaa caacgatagc cgctccgcac atcactgtcc ccggaaaggc
     1021 tgcggacttc attgcggctg ccggtattga ccgaacaggt cgcgggcgag gtcgaagatc
     1081 cggctgcggg gctgtttgtc caggcagtag gtgacgacgc cgtcgcactt gccctcgcgc
     1141 caggcttgca gacagaactt cagccactca gcgatgcgct cggggctggc cgggtccccg
     1201 tgccgcaact tgaactcgga agcatctccc gccgtcatga cgatgaaggg gacgtggaag
     1261 cgatgctcag gcgttttgat ccggtcgccg aacccggcct cgaaggcccc gcgctgggaa
     1321 acttgccacc tctgcggctg gtcgaggcca gcggcaagcg tcaacccttc cgcctgcaaa
     1381 tccttcacct gccagcggac gccgaagttg ctgacgcctt gcttgtccaa cagacgaaat
     1441 acaaccgtga gattggtttt accctgcgcg gcggcggtca catcaaccgc gacctcgccc
     1501 caggcgttgg tgccgccggc gacgtccgct tcccagacca cggtatcgtt caccagcagt
     1561 tgcttgaaat ggtagcccga ggtgcccgca gtgaagtcgt cccgctcgcg gaagcggaga
     1621 cgttggcgcc cgctcgacac ggctgtggcc gattggctgg cccggacgaa atcgcctggt
     1681 tgcgacgggg tgctcgctgg ataactcagt tcgcccgaca tggtcacggc tgcgtcattc
     1741 aagatggccc aggcctggtc aacctcgtcg cggtcttgcg ggtaagccac cacgaccccg
     1801 tcaatgacat cgcggtagtc ctgcacgaag gcgcgccgga tttcgccgaa gtacattagc
     1861 ggcaggaaga cgagccgggg attaatgctc ttggcccgct tttggaagtc gcgaacggtg
     1921 gcgggtgtaa agaacttatg gttggcgtag aaatcgtcaa tcacccaggc ggtcaggttg
     1981 gtatgctgga gcgagagccg cgcgatttcc tccgcccagc gcccgtagtc cagccgaaac
     2041 ggctcgggat acaggtcccc ttcccgcggc gcgctctcgg taggcgggac caaatagacc
     2101 cagacctgta tattcgcctg cgcggctttg ggcaggaaca gcttcaggtc gtcccaatcc
     2161 gttcgtgcat gccacaccag ccagtagtag gtggtgacgt gcagctcctt caggcgcgtc
     2221 aataggacgt cgccatccac gtgtccgtca gcacggcgcg gctcggaatc ataatcggcg
     2281 aaatggaacg gaggatggcg cggaccggct ggggccttgt cctgggcgag ggccaatcct
     2341 gcaaagccgc agagcaggga cagtgtgcat agggtggcga gagattgctt gggcgtccgg
     2401 gtgttcatcg tgatgcaggt ttcagcttca catggtttcc ggggaatcgg cctcggtgtc
     2461 aagcttgcgt gggctgacca gatcgctcag cgcgcgcccc agggtcaagt ttcggcggtg
     2521 agggcgcggg cgtggtgaat ttctttgctt ccgagggaac ggatctgatc ggcggagact
     2581 ttcgccacag cgaccgtcct tccgtcatag gtcgtgaatt cgaccttagc aggggtcagg
     2641 taatgagaat gtactattcc ccaccgcagt ccaagacgct gcgcgtgttc ggtggcgtcg
     2701 gaaggcgcgg gagcggcctg gaaggcgaaa acccctgagt gg
//
LOCUS       DATCGS010000312         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_571779, whole genome shotgun sequence.
ACCESSION   DATCGS010000312 DATCGS010000000
VERSION     DATCGS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571779"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VIL39_06380"
     CDS             <1..930
                     /locus_tag="VIL39_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5481089.1"
                     /translation="FVHELALKRVKFGLDILALVIPDTHGGYYPSLQRGFHTASSVLQ
                     NQVIVCDTDNDPLRQADALLQLLDKKVAGVALVPTTSPATPTHQLRPLLERGIPMVFC
                     HRRVEGIQAPLVTFCALEVGRLAGRAMITRHHRSVAFFSAQRAGLSMQYEQGLRQSLR
                     EGGGELSEEFVRCDTTAAATAEHELFLHQNLEEILSHPRRPSAIFCAFDSEAELVYLH
                     LQRMGISVPQEISVVGFGGTWREGALARRLTSVAVDEEELGRKAVGLLAEMQRRERPM
                     NDMTEMLMPLSLSDGETLGLAPKAGHPTEGKTL"
     gene            969..2069
                     /locus_tag="VIL39_06385"
     CDS             969..2069
                     /locus_tag="VIL39_06385"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY5481090.1"
                     /translation="MRPIIDAHLDLAWNALYFNRDLLASVADLRLAEQGMTDELARAR
                     NTLTLPELRRAKVAICLATLMGRSGPERPPRTGFKRTDLDYANQTIAYSHAKGQLAYY
                     RLLEQQGHLRFITTRGQLKAHWEAWTKSPSATPLGIILSMEGADSIMDPSQMPAWWND
                     GLRVVGPVHYGRSQYAYGTSTDGPLSDAGVKLLREFMRLGVILDVTHLSDRSFAHALE
                     VYSGPVLASHHNCRALVPADRQLTDEQIKKLVERGAVIGAAFDSWMLYPGWIRGKTNR
                     DLVGIEAAADHIDHICQLAGSARHCALGTDLDGGFGTEQTPRDLDTYTDVHKLEEVLA
                     GRGYRNVDIDAIFHGNWLRLFSQALPEQTAGS"
     gene            2089..3159
                     /locus_tag="VIL39_06390"
     CDS             2089..3159
                     /locus_tag="VIL39_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5481091.1"
                     /translation="MNRLPQRISLVVQTTSVLLEEIQGGRWVGSLPGEHALCAQLHVG
                     RKTLRAALEQLRQEGLLKCERGCRRKIVSRGAAGTTTRAMRVVLLMPAALQSLNPFVL
                     FLVDQLREYLAEEGYQLETHPSPALYRARIPQGMETLSRTLHPAGWVLLQSTETMQKW
                     FAARQLPCVVVGSRYAGVKLPSVDVDFDAVCVHAVNQFVSRGHKQLVLINPRAAAAGD
                     VRTEAGFREAVAQTRAPGIQAEVVEHDGTVSGICRRVGGLMARPHPPTAFLVSRAQYF
                     LTAVTRLLSSGVKVPGDVALISRDNDIPLEAIVPSVARYSQKPKTFAATASRLVMEII
                     RGTGKIAEHKIMPNFIHGQTLG"
BASE COUNT          656 a          988 c         1044 g          676 t
ORIGIN      
        1 ttcgtccatg agttggctct gaagcgggtc aagttcgggt tggacatcct ggcactggtg
       61 atccctgaca cgcatggcgg atactacccc tctctgcagc gtggcttcca cacggcgtct
      121 agcgtgttac aaaaccaggt cattgtctgc gataccgaca acgatcccct gcgacaggct
      181 gacgctctgc tacaactcct ggacaagaag gtggcgggag tcgctttggt gcccacgacc
      241 agtcccgcca cgccgaccca tcaattgcga ccgctccttg agcgagggat tcccatggtg
      301 ttttgtcacc gcagggtgga gggcatccag gcgccactcg tgacgttctg cgcgctggag
      361 gtcgggcggt tggccggtcg ggcgatgatc acgcggcacc atcggtccgt tgcatttttc
      421 agcgcgcagc gggctggctt gagcatgcag tatgagcagg ggcttcgcca atccctgcga
      481 gaagggggtg gagaattgtc ggaggagttt gttcgttgtg acaccaccgc agccgccaca
      541 gcggaacatg aactgttttt acaccagaat cttgaagaaa ttctgagcca cccgcgacgc
      601 ccctctgcga ttttttgcgc gttcgactca gaggccgagc tggtctattt gcatttgcag
      661 cgaatgggga tcagtgtgcc ccaggagata tctgtggttg gctttggggg tacgtggcgc
      721 gagggggctc tggcgcgacg tctaacctca gtggcggtgg acgaggagga actgggccga
      781 aaagcagtgg gcctgcttgc ggagatgcag cgacgcgaac gcccgatgaa tgacatgact
      841 gaaatgctga tgccgttgtc cctcagcgat ggcgaaacac tggggttggc gccgaaggct
      901 ggacatccaa ccgagggaaa gacgctataa ccctccaatt gacgccgtag gactattcgg
      961 atcgcccaat gagaccaatt attgacgccc acctggacct cgcgtggaac gcgctctatt
     1021 tcaaccgcga cctgctggcc agcgttgccg acctgcggct tgccgagcag ggaatgacgg
     1081 atgagctggc ccgggccaga aatacactta cgctgcctga attgcggcgt gcgaaggtcg
     1141 cgatctgcct ggccactttg atgggccgca gcggtcccga aaggccgccg cgcacgggct
     1201 tcaagcgcac cgatctcgat tacgccaacc agaccatcgc ttattcgcac gcaaaggggc
     1261 aactggctta ttaccgcctg cttgagcagc aggggcatct gcgcttcatt accacgcgcg
     1321 ggcaactgaa agcccactgg gaagcatgga ccaagtctcc gtctgccacg ccgcttggca
     1381 tcatcctcag catggaaggg gccgattcca ttatggatcc cagccagatg ccggcctggt
     1441 ggaacgacgg gttacgcgtc gtcggacccg tgcattacgg tcgcagccag tatgcgtatg
     1501 gcacttcgac tgacgggccg ctcagcgacg cgggcgtgaa gcttctgcgg gaattcatgc
     1561 gcctgggcgt cattctcgat gtgacccatc tctcggaccg atcttttgcc cacgccctgg
     1621 aggtttactc cggcccggtc ctggccagcc atcacaactg ccgcgccttg gtccctgcgg
     1681 atcggcagct cacggacgag cagattaaga aactcgtgga acgcggggca gtgattggtg
     1741 ccgcgttcga ttcgtggatg ctttatcccg gttggatacg cgggaaaacg aaccgagatc
     1801 tggtaggcat tgaggccgct gcggatcaca tcgatcacat ctgccaactt gccggctcgg
     1861 cccgtcattg cgccctcggc acggatctcg atggcgggtt tggcacggaa caaacgccgc
     1921 gcgatctcga tacctatacc gacgtccaca agctggaaga agttcttgcc gggcggggct
     1981 accggaacgt tgacattgat gccatattcc acggcaactg gctccgcctg ttcagccagg
     2041 cgctgccgga acagacggca ggaagttaag accgaaccgc tcaaatcaat gaaccgttta
     2101 ccgcaaagaa tctcgctcgt ggtgcaaacg acctcggttc tgctcgagga gatccagggt
     2161 ggccggtggg tggggtcact gcccggcgag catgctttgt gcgcgcagct ccacgtgggg
     2221 cgcaaaacct tgcgggcggc cctggagcag cttcggcagg agggcctgct gaagtgtgaa
     2281 cgcggctgcc ggcgaaagat cgtctcgcgc ggggccgcag gcacaacgac gcgggcgatg
     2341 cgtgtggtct tgctgatgcc cgcagcgctg caatccttaa atcctttcgt cctgttcctg
     2401 gttgatcaat tgcgggagta cctggccgag gaaggctacc agttggaaac ccatccgagc
     2461 ccggccctgt atcgggcgcg gattccccag ggaatggaga ccctgtccag aacattgcat
     2521 cctgccggct gggtcttgtt acaatccacc gaaacgatgc agaagtggtt cgcggctcgc
     2581 caactcccgt gcgtcgtggt gggttcccgt tatgcgggcg tcaagctgcc gtccgtcgat
     2641 gtggattttg acgccgtgtg tgtccatgcc gttaaccagt ttgtctcgcg cggccacaaa
     2701 caattggtgc tgataaatcc tcgggccgct gccgccggtg atgtccgaac ggaagccggt
     2761 ttccgcgaag cggtggccca aacaagagcg cctggaattc aggccgaggt ggtggagcat
     2821 gatgggactg tctccggcat ctgccggcgg gtgggcggcc tgatggcgcg gccgcatccg
     2881 cccaccgcgt tcctggtatc ccgggcgcaa tattttctca ccgccgtgac ccgcctattg
     2941 agctccgggg tgaaggtgcc gggcgatgtg gcgttgatat cacgcgacaa cgacatcccg
     3001 ctcgaagcga tcgtgccgag cgtggcccgg tactcccaga agccaaagac gtttgccgcc
     3061 accgcctcgc gcctggtcat ggagattatc cggggcacgg ggaagatcgc cgagcacaaa
     3121 atcatgccca atttcatcca cggccagacg cttggctgac cgccggccgc ttaaaaacag
     3181 cgaccgagcg aactccggtg gtttggtcac tagctgttcc cttaggagac agaacagttg
     3241 tggttgtaaa cctcggtcct cgcggctaaa cttcagcagt attgattcac tagactagaa
     3301 atttagcatg gcttactaca agcaacaaag catgcattat gggcggcctc gtaacgcctt
     3361 tacg
//
LOCUS       DATCGS010000313         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_423375, whole genome shotgun sequence.
ACCESSION   DATCGS010000313 DATCGS010000000
VERSION     DATCGS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423375"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            450..677
                     /locus_tag="VIL39_06395"
     CDS             450..677
                     /locus_tag="VIL39_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481092.1"
                     /translation="MKKTTGTAAEPEMRAEYDFSHGVRGKYARRYAQGANVVVLEADV
                     AAVFPNAEAVNDSLRALAGIIRRQHKALAGK"
     gene            complement(869..1681)
                     /locus_tag="VIL39_06400"
     CDS             complement(869..1681)
                     /locus_tag="VIL39_06400"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEY5481093.1"
                     /translation="MQARRVHQYVAGRVLWLAVAALLLRPLGVVGAALETFDVLQIGT
                     RTYTNVTVTTKATNYIFIVHKGGMVSLKVADLPPGVRQQLGYAGTPVSKAATNAAAVW
                     VKKGIARMDVPQIKHLRKRLEENFRGPAAAKLSAMHLASPRLIFVALGVSLLLYLFYG
                     YCLMLICRKTGNPPGALVWLPVLQLFPMLRAAGMSGWWFLAYLVPGLNLVAQVVWSVK
                     IAKARRKSVWIGILLLLPVTNLFAFLYLAFSESASGEEDEGPEPKVMSLLTV"
     gene            1861..4257
                     /locus_tag="VIL39_06405"
     CDS             1861..4257
                     /locus_tag="VIL39_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY5481094.1"
                     /translation="MNSSNNSRELVHPLSGIKRLLAVWVSAAALMAASAATISPLPDK
                     VAPVVPDRQDFQTPDRVRLTGWVGMRIEANEANRLVKLDPARLLEGYRKRPGRQSWDG
                     EHVGKWLHAATLAWVNTGDPALRQKLDYAAAELITCQLDDGYLGTYLEKDRWTEWDVW
                     AHKYNLIGLITYMRYTGNLEPLPACRRMADLLCQTFGDEPGKRDIIKAGEHVGLAPTS
                     VLEPMVLLYRLTGEPRYLDFCRYIFRAWEQPNGPKIISTLLTAKRVDKVGNGKAYEML
                     SCLNGALEFYRTTGEPKILEACLNGWADIVHNRLYLTGAASYGELFHDDFDLPNVANV
                     GETCVTVTWLQFNAQLLRLTGEARFAEQLEHVVLNQLFGAQRCDGSGWGYYVQMEGKK
                     PYSSSLDGHCCLSSGPRGVALIPAFAVTTDSDGIVVNLYDSGTAKVNLRNATAVTLTT
                     DTRYPSDERIQMTIDPAEQKTFAVKVRIPAWCGNSSLRVNGKPVTVVTGADGYVAVTR
                     KWKHGDRIELRFKLEPRVVVGDHKNQGKVAVLYGPLVLAADEALLGATNRSLNAISAA
                     GTNLAALGVRPEPAPDVVKSWPGAQVFRVNAIARKRAGSLKPGTPLQVRLIPFADAGA
                     NGTRYKVWLPLGLASSSGNMLLDGRESSSRIGNVSGSIIDEDLQSFVVTFNNKLRAED
                     WFAVALDEPVTVSRVIFAHGQTFHDGGWFDASTGKPHIQVKSAKDAAWETAGELADYP
                     ATTATDSAGLKGGERFNCQFAKPVTVVAIRVLGKPASGDNPQQAFASCAEIQAFNGSR
                     "
     gene            4294..4914
                     /locus_tag="VIL39_06410"
     CDS             4294..4914
                     /locus_tag="VIL39_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5481095.1"
                     /translation="MKTIVLKLFPALLLACLTFTRPCLAADAAKDGGWEPLFNGKDLS
                     GWVPVHDVTFVVTNGNLRLVTGTGWLRTEKQYGDFVLEAEWRALVPEYDSGFFIRAGL
                     EGKPWPKDGWQVNLNRNALGGLVKGYKTVVPAETPPMPLNKWVKFRMEARGRKITLDV
                     DGERAWEFKGLDADRGYIGIQAEGKAFDFRNIRIQELPHSDGAGQR"
BASE COUNT         1085 a         1699 c         1642 g          968 t
ORIGIN      
        1 agcttcggcg tctagccgcc ggggaatcag ccctggcatt gaacgacaag ccgccgccca
       61 agggctgacg ctgctgccag ccgggtgcat tccagctccg gcggggccag gcccaaatcc
      121 gtccgggtcg agaggcggga cggcgccgga agcggtccag accaccccgg caatccaaat
      181 tctgtcagca ggtcaattcc ttggcaggcc agcaagaatc tgggaatagt cagggcttgt
      241 taatccggcc cgtttcggcc agtctcgacc cacttcctgg ggaagcctat ttgaggagaa
      301 ctgtatgaaa agactattcg cggttggctt ggctgtgggt ttgctggctc gggttggctc
      361 tgcggtcgta attcccgtca actgcaacct cttcttcaac ggcagcaacg ccctccaaat
      421 cgcctggaat gcctatcctg gcaagagcta tgaagaaaac aactggcaca gcagccgaac
      481 ccgaaatgcg ggcggagtat gatttctcgc atggcgttcg tggcaagtat gcccggcgtt
      541 atgcgcaggg cgcaaacgtg gtcgtgctgg aagctgacgt ggcggcggtc ttccccaatg
      601 ccgaggcggt gaacgattca ttgcgcgcac tggcgggcat catccgccgg cagcacaagg
      661 cgctcgccgg caagtaagcc tgttgcctgc atgagccggc atctgcccgt cccggccacc
      721 caaagccaca tcaaagccac cccaaggcct gttgataggc aactgatagg caccccaaag
      781 ccaccccaag gctaccccaa ggctacccca aagccatctt aaggcgcggg gcctcagggc
      841 cgattccgca ttccatctct tgagcagctc agaccgtcag cagagacatg actttcggct
      901 cgggaccttc gtcctcttct ccggatgcgc tttccgagaa ggcgaggtac aggaaagcga
      961 agaggttggt aaccggcagg agcagcagaa tcccgatcca gacgctcttg cggcgcgcct
     1021 tggcaatctt cactgaccaa acaacctggg ccacgaggtt gagccccggc acaagatacg
     1081 ccagaaacca ccagcccgac atgccggcag cgcggagcat cggaaagagc tgcaacacag
     1141 gcagccagac cagagcgccc ggcggattgc cggtcttccg gcaaatcaac attaagcaat
     1201 aaccgtagaa caggtaaagc agcaggctga ctcccagcgc cacgaagatc agcctggggc
     1261 tggccagatg cattgcggac agcttggcag ccgctggccc gcggaagttc tcttccaggc
     1321 gctttcttag gtgcttgatt tgcggcacat ccatcctggc gattcccttc ttcacccaga
     1381 cagccgccgc gttggtcgct gccttcgaga ccggggtgcc ggcatagccc agttgctgcc
     1441 gcacgccggg gggcagatcg gcgaccttga ggctgaccat gccaccctta tgcacgatga
     1501 agatgtagtt cgtggctttc gtggtcactg tcacattcgt gtacgttcgc gtcccgattt
     1561 ggagcacatc aaacgtttcc aacgccgctc ccacgacccc gagaggccgc aataacaagg
     1621 ctgccaccgc cagccataac acccggccgg caacgtactg atgaactcta cgggcttgca
     1681 tacgctgtaa acattccgca ctgaacaaga ttgaacggat accagcgttg ggaaaccgca
     1741 cgacccgtgc catgcgcgcg gaaaccgcgc tcgccggctt tggacggttt tgcctttccc
     1801 cgtctcgtgc ctcacgctag ggtttccccg acctggcagt ttcaatcgaa ctgagaactt
     1861 gtgaatagca gcaacaactc cagagaactc gtccatccgc tctccggtat caaacgcctc
     1921 ttggcggtgt gggtttcggc tgcggcgctg atggcggcat cggctgcgac catttcgccg
     1981 ctccctgaca aggtcgcgcc cgtcgtcccg gaccggcagg actttcagac gccggaccgg
     2041 gtgcggctga ccggttgggt cggcatgcgc atcgaggcca acgaagcgaa ccggctggtg
     2101 aagctggacc cggcacgtct gctggagggc tatcgcaaac gcccgggccg gcagtcctgg
     2161 gacggcgagc acgtcggcaa gtggctgcac gccgccacgc tggcatgggt caacacgggc
     2221 gatcccgccc tgcgccagaa gctcgattac gcggccgctg aattgatcac gtgccagctc
     2281 gacgacggct acctgggcac ctacctcgaa aaggaccgct ggaccgaatg ggacgtgtgg
     2341 gcgcacaagt acaacctgat cggcctgatc acctacatgc gctacaccgg caacctggag
     2401 ccgctgcccg cctgccggcg catggcggat ttgctctgcc agacctttgg tgatgagccc
     2461 ggcaagcgcg acatcatcaa ggccggagag cacgtaggtc tggcacccac cagcgtgctc
     2521 gagcccatgg tgctgcttta ccgcctgacc ggagagccgc gttacctcga cttctgccgc
     2581 tacatcttcc gcgcgtggga gcagccgaac ggcccgaaga tcatctccac gctgctcacc
     2641 gccaagcgcg tggacaaggt cggcaacggc aaggcttacg agatgctctc ctgcctcaac
     2701 ggcgcgctgg agttctaccg gacaaccggc gagcccaaga tcctggaagc ctgtctcaac
     2761 ggctgggcgg acatcgtcca caaccgtctc taccttacgg gcgccgccag ctacggcgag
     2821 ttgttccatg acgacttcga cctgcccaac gtggccaacg tgggcgagac ctgcgtcacc
     2881 gtcacctggc tgcaattcaa tgcgcagttg ctgcggctga ccggcgaagc gcgttttgcc
     2941 gagcagcttg aacacgtggt gctgaatcag ttgttcggcg cccagcgatg tgacggctcg
     3001 ggctggggct attacgtgca gatggagggc aagaagcctt atagcagctc cctcgacggt
     3061 cattgctgtc tctccagcgg cccgcgcggt gttgcgctca tccccgcctt cgccgtcacc
     3121 acggattcgg atggcatcgt cgtgaacctt tacgactcgg gcaccgccaa ggtcaatctc
     3181 cgcaatgcca ccgccgtaac gctgaccacc gacacgcgct atcccagcga cgaacgaatc
     3241 caaatgacca ttgatcccgc cgagcagaag acgtttgcag tgaaggtgcg aatcccggcc
     3301 tggtgcggga attcatcgtt gcgggtcaac ggcaagccgg tcacggtggt gaccggggct
     3361 gatgggtacg tcgccgtcac gcggaaatgg aagcatggtg accgcattga actgcggttc
     3421 aagctggaac cgcgcgtggt cgtcggcgat cacaagaacc agggcaaggt ggctgtcctt
     3481 tacgggccgc tggttttggc ggcagacgag gcgctgctcg gggcgacgaa tcgcagcctc
     3541 aacgccattt ccgccgccgg gacgaacctc gctgcgctgg gtgtcaggcc cgagccggcc
     3601 cccgatgtgg tcaaatcctg gcccggcgcc caggtcttcc gcgtcaatgc gattgcccgc
     3661 aaacgcgcgg ggagtctcaa gccgggcacc ccgttgcaag tccggttgat cccatttgcg
     3721 gacgccggcg cgaacggaac acgttacaag gtgtggcttc cactgggact ggcttcgtcc
     3781 agcggcaaca tgctgctcga cggtcgtgaa agttcttctc gcatagggaa cgtgagcggc
     3841 tcgattattg atgaggacct gcagagcttc gtggtgacct ttaacaacaa gctgcgagca
     3901 gaggattggt ttgcggtggc gctggatgag cctgtaacgg taagtcgcgt catatttgcc
     3961 cacggacaga cattccacga cggcggctgg ttcgacgcca gcaccggcaa accccacatc
     4021 caagtgaaat ccgccaagga tgcggcgtgg gaaaccgcgg gtgaacttgc tgattacccc
     4081 gccacaaccg ctaccgactc cgccggcctc aagggcggcg aacgattcaa ctgccaattc
     4141 gcgaaaccgg tgacagtggt tgccatccgg gtgctcggca agcccgcctc cggtgacaat
     4201 ccacaacaag cctttgcatc ctgtgccgaa atccaagcat tcaacgggtc acgctgaccc
     4261 aggttataaa tcccaagctt aagattaccg cttatgaaga cgatagtatt gaaactgttc
     4321 cctgcccttc tgctggcctg tttgaccttc actcgaccct gcttggctgc cgatgcggcg
     4381 aaggacggcg gctgggagcc gctattcaac ggaaaggatc tgagcggctg ggtgcccgtg
     4441 catgacgtca cctttgtcgt caccaatggc aatctccgcc tggtcaccgg cacgggctgg
     4501 ttgcgcacgg agaagcaata cggggacttc gtgctggaag ccgagtggcg cgcgctggtg
     4561 ccggaatacg acagcgggtt tttcattcgc gccggactgg agggcaagcc ctggcccaag
     4621 gacggctggc aggtgaacct caaccgcaac gcgctcggcg gcctggtgaa aggctataag
     4681 accgtggtgc ccgccgagac gcccccgatg ccgctgaaca aatgggtgaa gttccgcatg
     4741 gaagcccggg gccggaagat cacgcttgat gtggatggcg agcgggcctg ggagttcaaa
     4801 gggctggacg ctgaccgcgg ttacatcggc atccaggccg aaggcaaggc gttcgacttc
     4861 cgcaatattc gcatccagga attgccccac agcgacgggg ccggccagag gtgagccgcc
     4921 cactacccaa agagaacagg tggattgctc caaacgcctc tcacccagat gcctgtaaag
     4981 aggagcgtcc tcgagagggg cgcgcaggca aacaagccga cggaagtgcc aaaacacgca
     5041 tggcgtatct ggggcgcgtg ttcacccagc accagcgaga tgaaaaaggc cacccggtgc
     5101 gcgactacga atcgaccacc tatgtctcgg gcatgggcct tcggatttcg cctcccctgc
     5161 cctgcccttc ccgccgacgg cgcttctttg caagaccttt ggccacggct ggcataggct
     5221 gtcggcgcta tgtcaactga accgagtttt cacatgtctc cggaggaatt ccgccgctgg
     5281 ggttacgcgg tggtggattg ggtggccgac taccagcggc gggtggcgac gctgccggtc
     5341 tgcgcgccgg ttgaaccagg gcaaatccgc gcgtcgttgc cggcggaacc gccg
//
LOCUS       DATCGS010000314         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_523034, whole genome shotgun sequence.
ACCESSION   DATCGS010000314 DATCGS010000000
VERSION     DATCGS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523034"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..3021)
                     /locus_tag="VIL39_06415"
     CDS             complement(43..3021)
                     /locus_tag="VIL39_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY5481096.1"
                     /translation="MLNDRSQNFVTRGLSPCCGEPIRAGLKRLALVLISLAATLPVSS
                     ASYYTQRLEDPRAVYIAGPSGGDDTTALQQAIDRVQETSGQGIVFLGPGRYRINDTLH
                     IWPGLRVIGYGATRPVIVLPANTKGFEEPSREKVMFFFAGGRPGFGRGRPRNPEAART
                     PVPDASPGTFYSALSNLDIEIEGGNPGAVAVRSRYAQHCFLAHMDLRLGSALAGIHEG
                     GNVVEDVRFFGGRYGIWTSKPSPGWQFTVIDCSFEGQREAAILEREAGLTLIRPHFLR
                     VPTAVALEPGKSDELWVKDACLEEVSGPAFLFGVESNPRNEINMEGVACRAVPVFAAL
                     RETGKHFAAPADTYVVKTFSHGLHYSDIGAIPQIRTLFDAAPLATLAPRVASDLPDLA
                     PRDTWVNLHDLGAKGDGTTDDTEVIQKALDTHPALYFPSGFYVVRDTLTLRSNTVMIG
                     LHPGATQIILQDNTAGYQGVGAPKALLEAPKGGSNIVIGLGLYTSGANPRAVAALWKA
                     GAGSMMNDVRFLGGHGTPKPDGSRENPYNSNHTSDPNPNRRWDSQYPSLWVTDGGGGT
                     FLDIWTPSTFAQAGMLVSDTETEGRVYQMSSEHHVHHEVQVRNAAHWRFYALQTEAER
                     GESGLDLPLEIESSRDISVANLHVYRVISTFQPFPWAIKVSNSRDIRFRNVHCYSNSK
                     VSYDSAVFDQTHNVEIRQREFAWLDLSGSAPQPKPIGRSPVVARGAVEKLAGGFYNIS
                     GGAAGPQGEFYFVDAHPQRIYRWNAGSRQVSILTNVPPEPINLAVDKSGNLVMVSYVG
                     KGAVYALKPQGATVPLLPETVATRAGKTFFLPVSDWRLNRESLSRPTAHFVSPDGTTI
                     LPAGADFLSGATSWGVKSSPQIRSFGLTSVMPGQPFYVTDEAELTTWAAAVNPDGSLA
                     DFRLFAEQGGEGVAVDSRGNVYIAAGQVHVYNPAGKLIDTIEVPERPLQLVFGGADHR
                     TLFVPARTSLYSVRMRYPGR"
     gene            3208..3489
                     /locus_tag="VIL39_06420"
     CDS             3208..3489
                     /locus_tag="VIL39_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY5481097.1"
                     /translation="MASATLNVSLSKEQLALVNREVDSGQYISAGEVVREALREWLNR
                     RLEADVAALEKNHKGAWERDTTPEEEALILAAKREARAELLARNKKTRK"
BASE COUNT          714 a         1101 c         1257 g          679 t
ORIGIN      
        1 tcggtgccga gtggccaaga cacttagttc gagcttacca gctcagcggc cggggtagcg
       61 catgcgcacc gagtagagcg aggttcgggc gggaacgaaa agcgtccggt ggtcagcgcc
      121 tccgaagacg agctgcaagg ggcgctcggg cacttcgata gtatcaatta gcttgccggc
      181 ggggttatag acgtggacct ggccggcggc gatgtaaacg ttgccgcgcg agtccaccgc
      241 cacaccttcg ccgccctgct cggcgaaaag gcggaagtct gccagactcc cgtctggatt
      301 cacggcggct gcccaggtcg tgagttcggc ttcgtccgtg acgtaaaacg gttgcccggg
      361 catgaccgat gtgagaccga aggatcggat ttgcgggctc gatttgacgc cccaactcgt
      421 tgccccgctc aggaaatccg ctcccgcggg gagaatcgtc gtgccgtcgg gcgagacgaa
      481 atgggccgtc gggcgtgaga gcgattcgcg attcaagcgc cagtcgctga ccggcaagaa
      541 gaacgtcttt cccgcccgag tggcaacggt ttcgggcagc aaaggcaccg tggcgccttg
      601 gggcttaagg gcataaacgg cgcctttccc cacgtaagac accatcacca ggttgccgct
      661 tttatccacc gccagattga ttggctccgg tgggacgttg gtgaggatgg agacttggcg
      721 cgacccggcg ttccaacgat aaatgcgctg cgggtgcgcg tccacgaagt agaactcgcc
      781 ttggggaccc gcggccccgc ccgaaatgtt gtagaagcca ccagcgagct tttccactgc
      841 accccgcgcg accaccggtg agcgcccgat cggcttgggc tgcggcgcgc tgccggagag
      901 gtcaagccag gcgaactcgc gctgacggat ctcgacgttg tgggtctggt cgaagacggc
      961 ggagtcatag ctcactttac tgttgctgta acaatgcacg ttgcgaaacc ggatgtcgcg
     1021 tgagttggat accttgatcg cccaagggaa gggttgaaac gtgctgatga cgcggtacac
     1081 gtggaggttg gcgacgctaa tgtcgcggga ggattcgatc tccagcggca ggtcaaggcc
     1141 gctttcgccg cgctccgctt ccgtctgcaa ggcgtagaaa cgccagtgag cggcgttgcg
     1201 gacctggacc tcgtggtgga cgtgatgctc gctcgacatc tggtaaacac ggccttcggt
     1261 ctcggtgtcc gagacgagca tgccggcctg agcgaacgtc gagggggtcc aaatatccag
     1321 aaacgtgccg ccgcctccat cggtgaccca aaggctgggg tattgactgt cccaacggcg
     1381 attgggattg gggtcggacg tatggttgct gttgtagggg ttttcgcgtg agccgtccgg
     1441 cttgggcgtg ccgtggcccc cgagaaagcg gacgtcgttc atcatcgagc cagcgccggc
     1501 cttccagaga gctgcgaccg cgcgcgggtt cgcgccgctg gtgtaaaggc cgaggccgat
     1561 cacgatattg ctcccccctt taggcgcttc gagcagggct tttggcgcac ccacaccctg
     1621 atagcccgcc gtgttatctt gcaggataat ctgcgtcgcc ccgggatgga gaccgatcat
     1681 tacggtgttc gaccgcagcg tcagcgtgtc gcgcacgacg tagaagccag aggggaagta
     1741 gagcgccggg tgcgtgtcga gggccttttg gatcacctcc gtgtcatcgg tggtgccgtc
     1801 gcccttggcg cccaggtcgt gcaggttcac ccaggtgtca cgcggcgcca aatcgggcag
     1861 atcggaggcc acgcgcggcg ccagggtggc gagcggagcg gcatcgaaca aggtcctgat
     1921 ttggggaatg gccccgatat cggaatagtg gaggccgtga gagaaggtct tcaccacata
     1981 ggtgtccgcg ggggccgcga agtgtttgcc ggtttcgcgc agcgcggcaa agaccggtac
     2041 cgcgcggcag gcgacccctt ccatgttgat ttcgttgcgc ggattgctct ccacgccgaa
     2101 gagaaaggcc gggccggaga cctcctcgag gcaggcgtct ttgacccaaa gctcatcgga
     2161 cttgccgggc tcgagcgcga cggccgtcgg cacgcgcaga aaatgggggc ggatgagcgt
     2221 cagtccggct tcgcgctcca ggatggcagc ttcgcgctgc ccttcaaagg aacagtcaat
     2281 caccgtgaac tgccagcccg gcgagggctt cgaagtccaa atgccgtagc gcccgccgaa
     2341 gaagcggacg tcctcgacga cgtttccccc ttcgtggatg ccggccagtg ctgagccgag
     2401 acggaggtcc atgtgggcga gaaagcagtg ctgggcatag cgtgagcgca cagccaccgc
     2461 ccctgggtta cccccctcga tctcgatgtc gaggttggag agcgcggagt aaaaggtgcc
     2521 gggactcgcg tcgggaacag gagttcgggc cgcctccgga ttgcgcgggc ggccgcggcc
     2581 gaagccgggc cgccctccgg cgaagaagaa catcaccttc tcgcgcgaag gctcttcaaa
     2641 gcccttggtg ttcgcaggga ggacgatgac cgggcgcgtc gctccatagc cgatcacgcg
     2701 caggcctggc cagatgtgaa gggtgtcgtt gatgcgatag cgtcccggcc cgaggaacac
     2761 gattccctgg ccgctggtct cctggacccg atcaatggct tgctgaagcg ccgtggtatc
     2821 atcaccgccc gatgggccgg cgatatagac cgccctggga tcctccagtc gctgcgtata
     2881 gtatgaggca gagctgaccg gcagcgtagc ggcgagcgag atcagaacca gggcaagacg
     2941 tttaagaccg gcccgaatcg gctctccgca acacggcgac agcccgcgcg tgacgaagtt
     3001 ttggctcctg tcatttagca tggccgcagc ctaccagggc tgcggggaga cgtcaaaagc
     3061 tgcgattcag attccgggcg ggtctgccgc caagggtgct gccgtcgcac caggtggaac
     3121 aggaagttct ggatcacctt gacagcaaat gactgctttc cacccacctc ttgtcaattc
     3181 ctggctggga aagtattgtg ggggtgtatg gcaagcgcga ctctaaatgt cagtctaagc
     3241 aaagagcaat tggccctggt aaatcgggag gtagattccg gacaatacat ttccgccggc
     3301 gaggtggtgc gcgaagcgtt gcgcgagtgg ctaaaccgcc gtctcgaagc cgacgttgcg
     3361 gcgcttgaga aaaaccacaa aggcgcctgg gaacgggaca cgacgccgga agaggaagcc
     3421 ctaatcctgg ctgccaaacg tgaagcccgc gctgagttgc tggccaggaa caagaaaacc
     3481 cggaaatgat tccggttgtc ctcgatgcca gcgtggtggg cgccgcttgc gggtggagat
     3541 ctgaaggcgc gttatcactc gcgatccgga cttgctcgaa ttggaaaagc catttggaat
     3601 ccagattctg acaccgcggg cattcctcag tcggctgcag tccggacttt gaccccgctg
     3661 gcaggagtgt caacccgtgg gcggcggctt atccctcaat gccaaggctg attcctcggc
     3721 ggccagacgc cgaagctcct cggcattgcc g
//
LOCUS       DATCGS010000315         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_523115, whole genome shotgun sequence.
ACCESSION   DATCGS010000315 DATCGS010000000
VERSION     DATCGS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523115"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1959
                     /locus_tag="VIL39_06425"
     CDS             142..1959
                     /locus_tag="VIL39_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEY5481098.1"
                     /translation="MMHRIFACTTLLCCAIFLTTPCFGQAASGIPRLQKQGTVTQLVV
                     DGKPFLALTGELGNNTATSLESMRPVWPQLVSGNLNSVLAAISWAQMEPEQGKFDFAV
                     IDGLIQEARKNNLKLVFLWFGSWKNGLSSYAPYWVKKDFEKYPRIQLRNGKTIELLSA
                     LSDATRDADARAFRALMRHIREVDGKQHTVLMMQVENEVGVLRDSRDRSAVANKAFAG
                     PVPKELMDYLVKNKANLIPEFRDLWAANGNKTSGTWEQVFGPGLPENIQMPIQTSTPP
                     MSREEHDTAWRKLSWPVDEIFMAWHYAKYINKIIEEGKAEYPIPMFVNAWLQQPNMAW
                     PGTYPSGGPLPQVHDIWRAGAPSSDLWAPDLYISQFEETCQRWIRNGNPLFIPETSAN
                     ATNAVLAFTKYGAIGYSPFGIERNVGPDTTLAGAYRLLGDMAPAILAHQGKNTMALVQ
                     MKEGDPPQKLSLGNYTLELTYMGSRRAPIAPAPPTPAGQTPAPGRGQVAPAGQPAPAG
                     GRGAGRGGAQSSTDVLAIFIANGPDEYFMGGTNGLRVTFTPNTPGPPVVGQGDVQVGR
                     FVDGKWTVVRQLGGDDTGQGEILSLRPNTVQRVTVYRYK"
     gene            1962..>2859
                     /locus_tag="VIL39_06430"
     CDS             1962..>2859
                     /locus_tag="VIL39_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481099.1"
                     /translation="MQHKNVRPDRRSFLLGIGAAVAGASSLEGQPAPAPSLPDPLREP
                     AGITAFAEGRSTVVRLTRSGAKWQGNDVEVSTELRKGKSGTEMAILVSAPKTRLTRIR
                     LRWHGAFPAGSRFLGDHWERSYGDLEWRGLSGERLMPWYFLASSGALTHGYGVKTGAA
                     SICHWQAGAGGVTLWLDVSNGGGGVELGERRLEAATLVVRKGQAGESSLGAARAFCRV
                     MCEKPLLPAKPIYGSNNWYYTYGRNVSAEASLKDAALMAEVIPAETANRPFVVIDMGW
                     GAARDGAGPVAETNKAYPDMPGL"
BASE COUNT          548 a          897 c          931 g          483 t
ORIGIN      
        1 tgatggccga cgagtgggaa cgggtggttc ttgccgaggg cagtagtgcc gctcgtcagc
       61 cgtaagcagc cgcgttgacc gaagagctgc ggtgaagtac gatgattttg gcgttaacgg
      121 ttccacatct aggagaccat catgatgcac agaattttcg cctgcacgac cctgttgtgc
      181 tgtgccattt tcctgaccac cccgtgtttc ggacaagccg cctccggcat ccctcgctta
      241 cagaagcagg gaacggtgac gcagttggtc gtagacggta agcccttcct ggcactcacc
      301 ggggaactgg gcaacaacac cgctaccagc ctcgagagca tgcggcccgt ctggccgcag
      361 ctcgtttccg gcaacctgaa ctccgtgctg gcggccattt cctgggcgca gatggaaccc
      421 gagcagggca agttcgattt cgccgtgatc gacggcttga ttcaagaggc ccgcaagaac
      481 aacctcaagc tggttttcct gtggttcgga agctggaaga acggcctctc cagctacgct
      541 ccctattggg tcaagaagga cttcgagaag tatccgcgca tccagcttcg caacggcaaa
      601 accatcgaac tgctgagcgc gctcagcgat gccactcgcg acgccgacgc acgggcgttc
      661 cgggcgctga tgcgtcacat ccgcgaggtg gacggcaagc agcacaccgt cctcatgatg
      721 caagtggaaa acgaggtggg tgttctgcgc gactcgcgtg accggtcggc cgtggcgaac
      781 aaggccttcg cggggcccgt gcccaaggaa ttgatggact acctggtgaa gaacaaagcc
      841 aacctgatcc cggagttccg cgatttatgg gctgccaacg gcaacaagac ctcagggacc
      901 tgggagcagg tttttggccc cggcctgcct gagaacatcc agatgcccat ccagaccagc
      961 accccgccga tgagccggga agagcacgac accgcttggc gcaagctgag ttggcccgtg
     1021 gatgagatct tcatggcctg gcactacgcc aagtacatca acaagattat cgaggaaggc
     1081 aaggcggagt accccattcc gatgttcgtg aatgcgtggc tacagcagcc gaacatggcg
     1141 tggcccggga cctacccgag cggcgggccc ctgccgcagg ttcacgacat ctggcgcgcc
     1201 ggtgcgccgt cgagcgatct ctgggcgccg gacctctaca tctcgcagtt cgaagaaact
     1261 tgccagcggt ggatccgcaa cggcaatcct ctcttcattc cggaaaccag cgcgaatgcg
     1321 accaatgcgg tcctggcctt caccaagtac ggcgcgatcg ggtactcgcc cttcggcatt
     1381 gagcgcaatg tggggccgga tactaccctg gcaggcgcct atcgtcttct tggagacatg
     1441 gcgcccgcga tcctggcgca ccagggcaag aacaccatgg ccctagttca aatgaaggag
     1501 ggtgatccgc ctcagaagct ttcgctgggg aactacactt tggagttgac ctacatgggc
     1561 tcccgtcgcg cccccatcgc accagcaccg ccaacgccgg cgggccagac tcccgcgcca
     1621 gggcgcggcc aagttgctcc agcgggacaa ccggctccgg ccgggggacg cggcgcgggt
     1681 cgaggagggg cccaatcctc cacggacgtg ctggcgatct tcatcgccaa cggtcccgat
     1741 gagtacttca tgggtggaac caatggcctc cgagtcacgt tcacccccaa cacgccgggc
     1801 ccgccggtcg tggggcaggg agacgtgcag gtgggcagat tcgtggacgg caaatggacg
     1861 gtggtccggc agttgggcgg cgacgacacc ggacagggcg agatcctctc gctgcgtccc
     1921 aacaccgtgc agcgcgtgac ggtctaccgc tacaagtaac catgcaacac aagaacgtgc
     1981 gtcccgaccg ccggtcgttt cttctgggca taggcgccgc ggtggccggc gcctcgtccc
     2041 tggaagggca gccggcaccg gcgccctcgc tgcccgatcc tctgcgcgaa cccgccggca
     2101 tcacggcttt cgccgaaggc cggtccacgg tagtccgcct gacccgctcg ggcgcaaagt
     2161 ggcaagggaa tgatgtcgag gtcagtacgg aacttcggaa ggggaagagc gggacggaga
     2221 tggccatctt agtaagtgcc cctaagaccc gattaacccg catccggctg cgctggcacg
     2281 gcgcttttcc ggccggctcg cggtttctgg gggatcactg ggagcgctcc tacggggatc
     2341 tggagtggcg ggggctctcg ggcgagcgtt tgatgccgtg gtacttcctc gcctcgagtg
     2401 gcgcgctgac gcacggctat ggagtgaaga cgggcgccgc ttccatctgc cattggcagg
     2461 ccggtgcggg cggagtcacg ctgtggctcg atgtaagcaa cggcggaggg ggagtcgaac
     2521 tgggcgagcg gcggctggag gcagccacgc tcgtcgtccg caaaggccag gccggcgaat
     2581 cctcgctggg cgcggcgcgg gccttctgcc gcgtgatgtg cgagaagccg ctgcttccgg
     2641 ccaagcccat ctacggcagc aacaactggt attacaccta tggccggaat gtcagcgcgg
     2701 aagccagcct gaaggacgcc gccctgatgg ccgaagtgat acccgctgag acggccaacc
     2761 gtcccttcgt ggtaatcgac atgggatggg gcgccgcgcg ggatggcgcc ggcccggtcg
     2821 ccgagaccaa caaagcctat cccgacatgc cgggactgg
//
LOCUS       DATCGS010000316         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_222811, whole genome shotgun sequence.
ACCESSION   DATCGS010000316 DATCGS010000000
VERSION     DATCGS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222811"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VIL39_06435"
     CDS             <1..1157
                     /locus_tag="VIL39_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5481100.1"
                     /translation="LMNCTSGYDTTQYVEPLGVFGGIAPWNFPAMIPMGWMAPLCIAT
                     GNTFVLKAASFTPQTSMRIAELWQEAGLPAGVINMLTCSRNEAEILLKHPDVKGISFV
                     GSTSVGRHIYSTAAAAGKRVQALTEAKNHALILKDAALERSARGIINSACGCAGERCM
                     ALPALVVEEEVADELVSLLAGMMKDLKIGAAYDKGSQLGPLVNEEHRNSVTRWIEKGV
                     EEGAKLVLDGRGVTVPGYEKGFYMGPTLFDHVEPGMTIGDREVFGPVLCIKRVKDFEE
                     GLALMNASEFANGSVIYTQSGYYAREFAKRTHGGMVGINVGIPVPLGIFGFTGHKNSF
                     FSDLHTMGTDGVRFFTELKSVTAHWFSEEEARAGRVLNSWDGMISMPASN"
     gene            1189..2109
                     /locus_tag="VIL39_06440"
     CDS             1189..2109
                     /locus_tag="VIL39_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5481101.1"
                     /translation="MIRIGNAPCSWGVLEFDLEGEAAGYVQVLNEMVETGYVGTELGD
                     WDFMPTDPDRLAAELTQTGLTMLGAFVPVQLKDPACHEPGVEAALKVARLLRATADRT
                     TGYLPYLVLADNNGSDRVRTRNAGRVTPEMGLSGEEWKVFAAGAERVARAVYAETGLR
                     TVFHHHCGGYAETPDEIERLLKMTDPQVLGLVFDTGHYAFAAGDCAAVVDGLNRFADR
                     IWYIHFKDCQPDIAARSRAEGWDYFESLRNGVFCELGKGGIDFPAVAAWLKERGYEGW
                     GIVEQDVLPGMGAPKEAARRNREYLKSIGL"
     gene            2126..>2752
                     /locus_tag="VIL39_06445"
     CDS             2126..>2752
                     /locus_tag="VIL39_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481102.1"
                     /translation="MAGIVNVGIIGARRIGKVHAQTLAYRIPSAKIQAVADLNAPAAG
                     QLAAQLSIPVATGNYRDVLENPDIQAVFICSATNTHAQFAEEAARAGKHIFCEKPIAL
                     NLKDIDCALEAVDRAGVKLQVGFNRRFDSNFYRVRLAIESGEIGKPALLHIISRDPAP
                     PPVSYVKVSGGMFLDMTIHDFDMARFLIGAEVEEVYALAGITVDLAIGE"
BASE COUNT          496 a          851 c          916 g          489 t
ORIGIN      
        1 ccctgatgaa ctgcacctcc ggctatgaca ccacgcagta cgtcgagccg ctgggcgtgt
       61 tcggcgggat cgcgccgtgg aactttccgg ctatgattcc catgggctgg atggcgccgt
      121 tatgcatcgc caccggcaat accttcgtgc tgaaggccgc tagcttcacg ccgcagactt
      181 ctatgcgcat cgccgagctg tggcaggagg ccggtttgcc ggccggcgtt atcaacatgc
      241 tgacctgctc ccgtaacgag gccgagatcc tgctcaagca tccagacgtg aagggcatct
      301 ccttcgttgg ctcgacctcc gttggccgcc atatctactc gaccgctgcc gcggccggca
      361 agcgggtcca ggcgctgacc gaggcgaaga accacgccct catcctcaaa gatgccgccc
      421 tggaacgctc cgcgcgtggc atcatcaact cggcctgtgg ctgcgccggc gagcgctgca
      481 tggcgctgcc ggcattggta gttgaagagg aggtcgccga cgagcttgta tcgctgctcg
      541 ccggcatgat gaaggacctg aagattgggg ctgcgtacga caagggcagc cagttgggcc
      601 cgctggtcaa tgaagagcac cgcaattcgg tgacccgctg gatcgagaag ggcgtcgagg
      661 aaggcgcaaa gctggtgctg gacgggcgcg gggtcacggt cccggggtac gagaagggtt
      721 tctacatggg cccgacgctg ttcgaccacg tcgagccagg catgaccatc ggcgaccgcg
      781 aggtgttcgg cccggtgctg tgcatcaagc gcgtgaagga cttcgaggaa gggctggcgc
      841 tgatgaatgc cagcgagttc gccaacggtt cggtgatcta cacccagagc ggctactatg
      901 cccgcgagtt cgccaagcgg acgcacggcg gtatggtcgg catcaacgtc ggcatccccg
      961 tcccgctcgg catcttcggc ttcacggggc ataagaattc gttcttcagc gacctgcaca
     1021 ccatgggcac ggatggcgtg cgcttcttca cggaactaaa gagcgtcacc gcccactggt
     1081 tctccgagga agaggcgcgg gcgggccggg tgctaaactc gtgggatggc atgatctcaa
     1141 tgccggcttc gaactaacgg acaaggtagt ctgtgtagga ggggtcatat gattcgtatc
     1201 ggcaatgcgc cctgtagctg gggggtgctg gagttcgacc tggaaggcga ggccgccggt
     1261 tacgtccagg tgttgaacga gatggtggag accgggtatg tgggaaccga gctcggtgat
     1321 tgggacttca tgccgaccga tccggacagg ctggccgcgg agctcacgca gaccggcctg
     1381 accatgctcg gcgcctttgt ccccgtgcag ctcaaggacc cggcgtgcca cgaacccggc
     1441 gtcgaggctg ccctgaaggt ggcgcggctg ctgcgcgcga ccgcggaccg gacgacgggc
     1501 tacctgccct acctcgtact cgccgacaac aacggcagcg atcgcgtccg gacccgcaac
     1561 gcgggccgcg tgacccccga gatggggctg agcggcgagg agtggaaagt gttcgcggcc
     1621 ggcgccgagc gcgtggcgcg tgcagtctac gctgagaccg ggctgcggac cgtcttccac
     1681 caccactgcg gcggctatgc tgagacgccc gacgagattg aacggctgct caagatgacg
     1741 gacccgcagg tgttgggcct ggttttcgac accggccact acgccttcgc cgcgggcgat
     1801 tgcgcggccg tggtcgatgg gctcaaccgc tttgccgatc gcatctggta catccacttc
     1861 aaggactgcc agcccgacat cgccgctcgt tctcgggccg agggctggga ctacttcgag
     1921 tctctgcgca acggtgtgtt ctgcgagctt ggcaagggcg gcatcgactt cccggccgtt
     1981 gccgcctggc tgaaggagcg cggctacgag ggctggggca tcgtcgagca ggacgtattg
     2041 cccggtatgg gggccccgaa agaggccgcg cggcgcaacc gcgagtatct caagagcatc
     2101 gggctctgat cagaagagaa agaatatggc tggcatcgta aacgtcggta tcatcggcgc
     2161 ccggcgcatc ggcaaggtcc atgcgcaaac tctggcatac cgtatcccca gcgccaagat
     2221 ccaggcggtc gcggacctca acgcccctgc ggcggggcaa ctggccgccc aattgagtat
     2281 cccggttgcg acgggcaact accgcgatgt gctcgagaat ccggacatcc aggcggtctt
     2341 tatctgctcc gcgaccaata cccacgcgca gttcgcggaa gaagccgccc gggcgggcaa
     2401 gcacattttc tgcgagaagc ccatcgcgct gaacctgaag gacatcgact gcgcgctcga
     2461 ggcggtcgat agggctggcg tcaagctgca ggttgggttc aatcgccgct ttgactcgaa
     2521 cttttaccgc gtgcgcctcg ctatcgagag cggcgaaatt ggcaaacccg ccctgttgca
     2581 catcatcagc cgcgacccgg ccccgccgcc cgtttcatac gtcaaggttt ccggtggcat
     2641 gttcctggac atgaccatcc acgatttcga catggcgcgt ttcctgatcg gcgccgaggt
     2701 cgaggaagtg tacgcgctgg ccggcatcac ggttgacctg gcgatcggcg ag
//
LOCUS       DATCGS010000317         3657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_523853, whole genome shotgun sequence.
ACCESSION   DATCGS010000317 DATCGS010000000
VERSION     DATCGS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523853"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1296
                     /gene="larA"
                     /locus_tag="VIL39_06450"
     CDS             10..1296
                     /gene="larA"
                     /locus_tag="VIL39_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEY5481103.1"
                     /translation="MKVNLAYGQGHLPVELPDGLTTLIEPSHTPGLADERGAVLAALA
                     KPIAARPLREWVKPGSRVCVVFTDITRATPNERLIPWLLGCLAEVPRDNITLLNALGT
                     HRANTRAELEQMLTPAVVRNYRVLNHEAENPAALIPLGTTGDGTPALLNRHFVEADVR
                     IVTGFIEPHFFAGFSGGPKGIMPGVAGLQTVMSNHSARNLGDPNSTFGVTKGNPIWEE
                     MRDIALRAGPSFLLNVALNEQRQITGVFAGDLLAAHRAGCEFVRQSAMQQFDAPFDVV
                     VTTNSGYPLDQNLYQGVKGMSAGARVLKPGGTLILACGCRDGVPANSSFDQILRSAAS
                     PEELLTLLATPGYRRPDQWQAQLQALIQCQARVLLYSSLSDDIVRAAHLTPCHDIGGA
                     VKELVAGHLNDARIAVLPQGPLVIPCLAQPTIPAGP"
     gene            1445..3505
                     /locus_tag="VIL39_06455"
     CDS             1445..3505
                     /locus_tag="VIL39_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY5481104.1"
                     /translation="MHFGVDYHPEQWVYPYGGTRERPEAVWERDVELMQTAGVNVVRM
                     GEFTWGLCEREEGKFDFAWLKRVMDLLGKAGIKVVLATPTAAPPIWLAQKYPEILPVD
                     ERGLIKHEGTRRAICLNSDVFWNHSKLIVENMAKALGQHPQLIAWQIDNGLGGNFTEA
                     AFNEDARREWHLWLEAKYETVERLNDLMGLRHWGQVVTSWNQVPMPMIAPAIHNPALV
                     LDWNRFCSDTIVQFVKMQADLLHQLSPKCPVTTNLRPLLHRFDHFDVAEAIDFVSIES
                     TAALKAKSAELACEIDMLRSLKKTDIRTPDGDTGFWVMEQKAGNVTWQDVNSLVRPGV
                     LRMFTYQLVSRGATAIIFFRWRQPRFGTEKFHGAVLPHNARSTARVFKEVAQLGDELK
                     LLAPSLKGTHVEAETCILYSHDNDWALQQPMQPNKFFSLREHIQLFYNALHDRNIAVD
                     FARPTEDLSRYKLVFAPSLHLLSGGEADRLKLYVQNGGTLVATFNTGLVDEHNMAPDN
                     GYPHDLTDLFGLEVLEFDQLPPGEENHLTFKGTFPASHMHPARLWCDLIEPRDCQVIA
                     TYAKDFYAGSPAMTLHTFGLGKAVYIGTQSHQHFYNDLVTWLRQMCNLHPLLKVPDNV
                     EVSMREKEDAQIFFLLNHQSSPLRIQFYKPMHDFLTGNTFSGNYDLPPHGVLVLDEHP
                     ETKT"
BASE COUNT          726 a         1157 c         1106 g          668 t
ORIGIN      
        1 aatgagtgaa tgaaagtcaa cctcgcttat ggtcagggcc acttgcccgt cgaactgccc
       61 gacgggctga cgaccctcat tgagccatcg cacacgcccg ggctggccga cgaacgcggc
      121 gcggtgctgg cggcgctggc aaagcccatc gcagcccgcc cattgcggga atgggtgaag
      181 cccggcagcc gcgtctgcgt cgtgttcact gacatcactc gcgccacgcc caacgagcgc
      241 cttattccct ggctgctggg gtgcttggcc gaggtgccgc gcgataacat taccctgctc
      301 aacgccctcg gcacgcaccg cgctaacacc cgcgccgaac tggagcaaat gctgacgccg
      361 gccgtcgtgc ggaactaccg cgttctgaat cacgaagcgg aaaatcccgc cgcgctgatc
      421 cctttgggca cgacgggtga cggcaccccg gcgctgctca accgccactt cgtcgaggcg
      481 gacgtgcgga ttgtcacggg ctttatcgag ccgcatttct tcgccgggtt cagcggtggc
      541 cccaaaggca tcatgccggg cgtcgccggg ctccagaccg tgatgagcaa tcacagcgca
      601 aggaatcttg gtgatcccaa ttccaccttt ggcgtcacca aaggcaaccc tatctgggag
      661 gaaatgcgcg acatcgccct acgcgcgggc ccgagcttcc tgctgaacgt ggccctgaac
      721 gagcagcggc agattaccgg ggtgttcgct ggcgacctgc tggcggctca ccgagccggc
      781 tgcgaatttg tgaggcagtc ggcgatgcag cagttcgacg cgccctttga cgtggtcgtc
      841 accacgaaca gcggctaccc gctcgatcag aacctgtatc aaggcgtcaa aggcatgagc
      901 gccggcgcgc gcgtgctcaa gccgggcggc acgctcatcc tggcctgcgg atgccgcgac
      961 ggcgtgccgg ccaacagtag cttcgaccag atcctgcgca gcgccgccag cccggaagag
     1021 cttttgaccc tgctggcgac gcccggttac cgccggcccg accagtggca ggcgcaactc
     1081 caggcgctca ttcaatgcca ggcccgcgtt ttgctctaca gctcgctgtc cgacgacatc
     1141 gtccgcgcgg cccatctgac gccttgccac gacatcggtg gggcggtcaa ggagttggtc
     1201 gcgggacatc tcaacgacgc ccgcatcgcc gtcctcccgc aggggccgct ggtcattccc
     1261 tgcctggctc aacccactat tcctgccgga ccatgattcc cgacccgagc cgcggcggcc
     1321 cgccggcagc ggcgattgcc acccaaaaaa cttgttccca gaaccacttt taacccactt
     1381 ttagattgcg ccagggtgga attgtattta cagtgcaaga aaggcagcag cgcgaaagga
     1441 caacatgcat ttcggtgtag attatcatcc agaacaatgg gtttacccat acggcggaac
     1501 ccgcgagagg cccgaagccg tttgggagcg tgacgtagag ttgatgcaga ccgctggcgt
     1561 caacgtggtc cgcatgggcg agtttacctg gggactctgc gagcgggagg agggcaaatt
     1621 cgactttgcc tggctcaagc gcgtgatgga cctgctgggc aaggcgggaa tcaaagtcgt
     1681 cctggccacg ccgacggctg cgccgcccat ctggctcgcc cagaagtatc cggagattct
     1741 gcccgtggat gagcgcgggc tgatcaagca cgaaggcacg cgccgggcga tctgcctcaa
     1801 cagtgatgtc ttttggaatc actccaagct gattgtggaa aacatggcca aggccctcgg
     1861 ccagcacccc caactcattg cctggcaaat agacaacggc ctgggcggca attttaccga
     1921 ggcggctttc aatgaggacg cgcgccggga atggcatctg tggctggagg cgaagtatga
     1981 gaccgtcgaa cggctgaacg acttgatggg tttgcgccac tggggccagg tcgtcacaag
     2041 ctggaaccag gtgccgatgc cgatgatcgc gccggcgatt cacaacccgg cgctggtgct
     2101 ggattggaac cggttctgca gcgacaccat cgtgcagttc gtcaaaatgc aggccgacct
     2161 gctgcaccaa ctcagcccga aatgcccggt cacgaccaat ttgcgcccgc tgctccaccg
     2221 cttcgatcac ttcgacgtgg ccgaggccat tgacttcgtc tcgattgaga gcaccgccgc
     2281 cctcaaagcc aagtcggcgg agctggcctg cgagattgac atgctgcggt cgcttaagaa
     2341 gacggacatc cggacgccgg atggcgacac gggcttttgg gtgatggagc agaaggcggg
     2401 caacgtgacg tggcaggacg tcaactcgct cgtgcggccg ggggtgctgc ggatgttcac
     2461 ttatcaattg gtgtcccggg gcgcgacggc gattatcttt ttccgctggc gccagccgcg
     2521 ttttggcacc gagaagttcc acggcgcggt cctgccgcac aacgcgcggt caaccgcccg
     2581 ggtgttcaaa gaggtcgccc aactcggcga cgagctgaag ctgctggccc cgtctctcaa
     2641 ggggacgcac gtggaggcgg agacctgcat cctctacagc catgacaatg actgggcgct
     2701 gcagcaaccg atgcagccaa acaagttctt cagcttgcgc gagcacatcc agctcttcta
     2761 caacgcgctg cacgaccgga acatcgcggt ggatttcgcg cggcctaccg aggatctctc
     2821 gcgctacaag ctcgtcttcg caccctcgct gcacttgctg tccggcgggg aagcggaccg
     2881 gctgaagctt tatgtacaaa acggtggcac gctggtcgcc acgttcaaca ccggcctggt
     2941 ggacgaacac aatatggcgc ccgacaacgg gtatccccat gacctgacgg atttattcgg
     3001 cctggaagtg ctggagttcg accagttgcc tcccggagaa gagaaccacc tcacgttcaa
     3061 gggcactttc ccggcgagcc acatgcaccc ggccaggctc tggtgcgacc tcatcgagcc
     3121 gcgagattgc caggtcatcg ccacctacgc caaggacttc tatgcgggca gcccggcgat
     3181 gacgctgcac acgtttgggc tcggcaaagc cgtttacatc ggcacgcaaa gccaccagca
     3241 tttctataac gacctggtga cctggctgcg ccagatgtgc aacctgcatc cgctgctcaa
     3301 agtcccggac aatgtcgagg tgagcatgcg cgagaaggag gatgcccaga tattcttcct
     3361 gctgaatcac cagagttcgc cgctgcgcat ccagttttac aagccgatgc acgatttcct
     3421 gaccggcaac acgttttccg gcaattacga cctgccgcca cacggcgtgc tcgtgctgga
     3481 tgagcatccg gaaaccaaga cgtgattcgt tggctggttg catgagttgc atgagttgca
     3541 tgagttgcat gagttacatg agttacctgg ggggggggcg ccgggctcat gcaaccatgt
     3601 aaccatgcaa ccatgcaacc tatgtacctt gtgtaaccta cacgaccagt tccagca
//
LOCUS       DATCGS010000318         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_127903, whole genome shotgun sequence.
ACCESSION   DATCGS010000318 DATCGS010000000
VERSION     DATCGS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127903"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="rfbC"
                     /locus_tag="VIL39_06460"
     CDS             <1..662
                     /gene="rfbC"
                     /locus_tag="VIL39_06460"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY5481105.1"
                     /translation="RGHSCPQQYPKARSASGMAVQYPQAGAAADRNVRAPLRACDMKF
                     SPTSLPGVWLVELELREDSRGFLARTYCEQEFTAQGLNTRWPQCNLTLTRERGMIRGM
                     HFQADPKPEIKLIRCAAGAIFDVLVDARRNSPAFGRWEGFELSAANRRQLYVPGGLAH
                     GVQCLTDNCEVFYMMSESYVPALARGLRWNDPTVNIRWPVADPKLSERDRNLPLLADL
                     P"
     gene            659..1567
                     /locus_tag="VIL39_06465"
     CDS             659..1567
                     /locus_tag="VIL39_06465"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5481106.1"
                     /translation="MRILVTGASGFIGSTFCRLALSRGHEIAGLMLPTETPPAHVPAS
                     EQMLWLKGTLADPPWAAIEHFEPQVCVHFAWIATPGVYLESPENESYLQWSLDLVHRL
                     RGLGTNHIVGVGTCIEYQITNAPLSEAHTPVDPTTLYSRCKNALRETLEAEARHDGWH
                     FCWGRVFYPYGVGEHPARLCSALIQKLRHGEKLVLKTPHSTKDYIYIEDLAAAILLTV
                     EKRITGTINWGTGLGISVRQIADMIAGMLGRSELVEEMSPAEKDPLGFVVADATRLKQ
                     LGWRQQFSLEQGLKHLQRSLRTLEPV"
     gene            complement(1657..2517)
                     /locus_tag="VIL39_06470"
     CDS             complement(1657..2517)
                     /locus_tag="VIL39_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481107.1"
                     /translation="MSEAAIVYLARARSGLESLQRFLRSYERHPGGLEHDLVVVFKGF
                     SQEAELAEWRTALTRFPHQELRMSDFGFDIRAYSLAARRLPHRYLCFLNSFSEPLTPD
                     WLRILHRWVVEPGVGVVGCTGAWTSISSIVEQELASAATQPLLRRLLLRIKVPINRRA
                     YPPFPNPHLRSNAFLVSRELMTRVWPGFVIGKRKAYHWESGRKGFTRRVQALNLRVLV
                     AGKDGRSYTIEEWPRSGIFLQGKQENLLVSDNQTRGYDNADAAKRRQLSMYAWGMDRT
                     SAREELPAEK"
     gene            complement(2526..3512)
                     /locus_tag="VIL39_06475"
     CDS             complement(2526..3512)
                     /locus_tag="VIL39_06475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5481108.1"
                     /translation="MATQKALVTGITGQKARPSVGVVIVNHNLKASLRETLQSVRQVH
                     YPSVTVVVSDNASSDGAPEMVRQEFPEVHLLAHEEEQGYARAASLGVAFLLDKVKYIF
                     STTNDVILDPEILNVLVDYAENHPEAGVLGSKILFFDRPDVIWHAGGRIHPLHGHSRH
                     LGWNRRDHPRYGRVRECDFVTGCGFLLRCEALKKTGFFKEDLVFYSEDAELCYRIRES
                     GYRVVYVPEAKMWHKVGTTLAKNRSLQLRYSTRNGLYLIQRHRVGWYPLSLWVHLLAV
                     SPFKMLVFALMLRGRNSVGIWQGINDWRQGRFGWLKEPTPPRVAKPPRRSNP"
     gene            complement(3606..>3831)
                     /locus_tag="VIL39_06480"
     CDS             complement(3606..>3831)
                     /locus_tag="VIL39_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481109.1"
                     /translation="VPDAILAGVVAAALAWLWDKQLGHATLLLKVGMVFLPGGVAVLV
                     YGLVALWLKVPAAQEMTDLARQRFRKVTKA"
BASE COUNT          711 a         1215 c         1218 g          687 t
ORIGIN      
        1 agcgcggaca ttcctgtccg cagcaatatc cgaaagctcg ctcggcctcc ggaatggcgg
       61 tgcagtatcc gcaggcgggg gctgctgcgg acaggaatgt tcgcgctccg ttgcgtgcct
      121 gtgacatgaa gttttccccc accagcctgc ccggcgtgtg gctcgtcgaa ctcgagttgc
      181 gcgaagactc gcgcgggttc ctggcccgga cgtattgcga gcaggagttc accgcgcagg
      241 gccttaacac ccgctggccc cagtgcaatc tcacgctcac gcgggagcgc ggcatgattc
      301 gcgggatgca cttccaggcc gacccgaagc cggagatcaa gctcatccgc tgcgccgccg
      361 gcgcgatctt cgacgtgctg gtggacgcgc gccgcaactc gcccgccttt ggccggtggg
      421 agggcttcga gctgtccgcc gcgaaccgcc gccagcttta tgtgccgggc ggcctggcgc
      481 acggcgtcca gtgccttacg gacaactgcg aggtctttta catgatgtcc gagtcctacg
      541 tgcccgccct cgcccgcggc ctgcgctgga acgatcccac ggtgaacatt cgctggcccg
      601 tcgccgatcc caagctctcc gagcgcgacc gcaacctgcc tctgctggcc gacctgccat
      661 gagaatcctc gtcaccggcg cctcgggttt catcggctcg accttttgcc ggctggccct
      721 gagccgcggc cacgagatcg ccggcctgat gctgccaacg gaaaccccgc ccgcccacgt
      781 gccggccagc gagcagatgc tctggctgaa gggcacatta gccgatccgc cttgggccgc
      841 gattgaacac tttgagccgc aagtctgcgt tcacttcgct tggattgcga cgccgggcgt
      901 ttacctcgaa tcgccggaga atgagagcta tttgcagtgg agcctggacc tggtccaccg
      961 gctgcgcggc ctgggcacga accatatcgt cggcgtcggc acttgcatcg aataccagat
     1021 caccaacgcc cccctgtccg aggcgcacac gccggtggac cccacgacgc tctactcgcg
     1081 ctgcaaaaac gcgctgcgcg aaacgctcga agcggaagcg cgccatgacg gctggcattt
     1141 ctgctggggt cgggtgtttt atccctacgg cgtgggcgaa catccggcgc gcttgtgcag
     1201 cgcgctcatc cagaagctcc gccacggcga gaaactggtc ctcaagacgc cccacagcac
     1261 gaaggattac atttacatcg aagacctcgc cgccgcgatc ttgttgacgg tggaaaagcg
     1321 cattaccggc acgatcaact ggggcacggg ccttgggatt tccgtccgtc agatagccga
     1381 catgatcgcc gggatgctgg gccggtcaga gttggtggag gagatgtcgc cagccgagaa
     1441 ggacccgctg ggttttgtgg tggccgacgc gacgcggctg aagcagttgg gctggcggca
     1501 acaattcagc ctggagcagg ggttgaaaca ccttcagcgc agcctcagaa cgctcgagcc
     1561 ggtataagcg atttgacggg agggcgcccg gtggggacac cgggcggctc acccaactgc
     1621 aacgcttgat cacaggagcg aatccgaggg gggcggtcac ttctccgccg ggagttcctc
     1681 ccgtgccgac gtccggtcca taccccaggc gtacatggat aactggcgcc gtttcgccgc
     1741 atcagcattg tcgtagccgc gcgtttgatt gtccgagacc agcagattct cctgtttgcc
     1801 ttggaggaag atgccgctgc gcggccattc ttcaatggtg taggagcggc catctttgcc
     1861 cgcaaccagc acacgcaggt tcagcgcttg aacgcgccga gtgaacccct tccgaccgct
     1921 ttcccaatgg taggccttgc gcttgccgat gacgaaaccg ggccagacgc gggtcatcag
     1981 ctcgcgggaa acgaggaagg cgttgctgcg caggtgaggg ttgggaaacg gcgggtaggc
     2041 gcggcggttg atgggaactt tgatccggag caggagtcgg cggaggagtg gctgggttgc
     2101 ggcagaggca agttcctgtt ccacaatgga agatatgctc gtccaggcgc ctgtgcagcc
     2161 gaccactccc acgcccggct caaccaccca gcggtggagt atccgcagcc agtccggggt
     2221 gaggggttcg ctgaacgagt tgaggaagca gaggtaacga tggggcagcc gccgtgccgc
     2281 cagcgaataa gcccggatgt cgaagccgaa atccgacatt cggagctcct ggtggggaaa
     2341 gcgggtcagg gccgtgcgcc actctgccag ctcggcctcc tgcgagaaac ccttgaacac
     2401 caccactaag tcatgctcca gcccgcctgg atggcgctcg taactgcgca ggaagcgctg
     2461 cagcgactcc agaccgctgc gcgctcgcgc gagataaacg atggctgctt cggacatgtg
     2521 actggttatg ggttggatcg gcgtggtggt tttgccacgc ggggcggggt tggctctttc
     2581 agccacccga accggccctg gcgccaatcg ttgatcccct gccagatgcc gacggagttc
     2641 cgtccgcgta gcatcagggc gaaaaccagc atcttgaacg ggctgacggc cagcagatga
     2701 acccagagcg acagtggata ccaaccgacg cggtggcgct ggatgaggta gaggccgttc
     2761 cgggtgctgt agcggagttg cagcgaccgg ttcttcgcca gcgtggtgcc caccttatgc
     2821 cacatcttcg cctccggcac atagaccacg cggtagccgc tctcccggat gcggtaacac
     2881 agctcggcgt cctcggagta gaagaccagg tcttccttga agaaaccggt cttcttgagc
     2941 gcttcgcagc gcaggagaaa gccgcagccc gtgacgaagt cgcactcgcg cacccggccg
     3001 taacgcggat gatcgcgccg gttccacccc aagtggcggc tgtgcccgtg caggggatgg
     3061 atgcggccgc ccgcatgcca gattacgtcc ggccggtcga agaacaggat tttgctgccg
     3121 agaacacccg cttcgggatg gttctcggcg taatccacga gcacgttgag aatctccggg
     3181 tcgaggatga catcattggt cgtgctgaag atgtacttca ccttgtccaa cagaaaggcc
     3241 acgcccaggc tggccgcgcg agcgtagccc tgttcttctt cgtgcgccag caggtgaacc
     3301 tccgggaatt cctgacgcac catttccggg gcgccgtcgg aggaagcgtt gtcggaaacc
     3361 accacggtga cagacggata atggacctgg cgcacggact gcaatgtctc gcgcagcgaa
     3421 gctttcaggt tgtgattgac gatcaccact ccgaccgatg gtctggcctt ctgcccggta
     3481 atgccggtta cgagggcctt ctgagttgcc atattggcag aaatgctttc acaaatcggc
     3541 gcctgatgac aagtcaatta cgacccatca gggccttcag acaaggtcct tggcatgaac
     3601 cggcgttaag ccttagtcac cttccggaac cgttgccggg ccagatccgt catctcctgg
     3661 gccgcgggga ctttcagcca gagcgccacc agcccatata cgaggacggc gacgcccccg
     3721 ggcaggaaga ccatcccgac cttgagcaat agtgtggcat ggccaagctg cttgtcccag
     3781 agccaggcga gcgccgcggc caccactccc gccaggatcg cgtccggcac c
//
LOCUS       DATCGS010000319         3460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_523864, whole genome shotgun sequence.
ACCESSION   DATCGS010000319 DATCGS010000000
VERSION     DATCGS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523864"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1180)
                     /gene="aroC"
                     /locus_tag="VIL39_06485"
     CDS             complement(98..1180)
                     /gene="aroC"
                     /locus_tag="VIL39_06485"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY5481110.1"
                     /translation="MSNTFGHLFRITTWGESHGGGVGVVVDGCPPRLPLTEADIQPDL
                     DRRRPGQSKIVSPRKESDTVQILSGTFEGKTLGTPICLWVRNEDARSEAYNEMATKFR
                     PSHADYTYQAKFGIRAWPGGGRTSARETVGRVAAGAIAKKILRRHYGVEVLAYVRQVQ
                     RIVATVDPEKVKAKDIESNIVRCPDPAAAQRMIRLIEKTRRAGDTVGGIVEGIARGAP
                     PGWGEPVFDRLEADLAKAMLSLPASKGFDIGSGFGSILLTGTQHNDAFRAKDGQVYTT
                     SNRSGGIQGGISNGQTIYFSVAFKPVATVMHEQDTVDIQFKNTTLKGRGRHDPCVLPR
                     AVPIVEAMTALVLVDHALRHKAQCGA"
     gene            complement(1349..1552)
                     /locus_tag="VIL39_06490"
     CDS             complement(1349..1552)
                     /locus_tag="VIL39_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004593271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HEY5481111.1"
                     /translation="MECKKSSNARFCNCTYPGCEKHGICCECLHYHLGMRQLPACCFP
                     KDVERTYDRSFERFAGLVTTGKV"
     gene            complement(1630..2238)
                     /locus_tag="VIL39_06495"
     CDS             complement(1630..2238)
                     /locus_tag="VIL39_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5481112.1"
                     /translation="MNYFSLPIGGNYLVRVFALLFSLMSLGRAYAAPTIVSTVPASGA
                     SGVALSAAVIITFSEAMNTGVTTASFLASGPYEYVPTSLSWSAGNTVLTCTPTAAWPA
                     NRTILWNVEGTPLGGYSGGTFTTGSGDQLTLTNAIWSGGAFAFDVTSPAGQTLTVEYS
                     STLRTNQWQTLLTTNSPAGLVHIMDPHSSTNRYLFYRARTGS"
     gene            2364..2846
                     /locus_tag="VIL39_06500"
     CDS             2364..2846
                     /locus_tag="VIL39_06500"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY5481113.1"
                     /translation="MLLGLLYGHRDPDGTISIAMRGGQDSDCNPSSAAGVLFTTMGFA
                     KLPERFTKDLNQTNIFSHTAYSFPRLIAVCEKLAREVVVKSGGRIEKRSGEEFFVLPV
                     RVPQTSKLELSWAPGPIANSRFTPAELAQIKVNNVPVHMPTNPPAGPSKRPIGPRLQW
                     "
     gene            2975..>3460
                     /locus_tag="VIL39_06505"
     CDS             2975..>3460
                     /locus_tag="VIL39_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5481114.1"
                     /translation="MYRDQRVVVVMPAYNAARTIRQTYAEVREQQVVDGIILVDDGSR
                     DETVSVARSLEGVQVHVHKVNKGYGGNQKTCYRLALEAGADIVIMIHPDYQYTPKLIP
                     AMVAIIGNGLHPCVLGSRILGGYALRGGMPLWKYVSNRCLTLVENVLLGAKLSEYHTG
                     YR"
BASE COUNT          649 a         1003 c         1133 g          675 t
ORIGIN      
        1 gcggtgccag gacacgcctg atttgttgac tgctcccgat gtgagagcaa gaccactccc
       61 cgccgatcag cagcgctgtt aattgcccac gaaggcttca cgcgccgcat tgcgccttgt
      121 gccgcagggc gtgatccacg agtaccaggg cggtcatcgc ttcgacgatt gggaccgcac
      181 gcggcaggac gcagggatca tgacggcctc ggcccttcag cgtggtgttc ttgaactgga
      241 tgtccaccgt gtcctgctcg tgcatgacag tagcgacggg tttgaaggcg acgctgaaat
      301 agatggtctg cccattcgag atgccgccct ggatgccgcc cgaacggttc gaggtagtat
      361 agacttggcc gtctttcgcg cgaaacgcgt cgttgtgctg cgtgccggtg agcagaatgc
      421 ttccgaagcc cgagccgatg tcgaatccct ttgacgccgg cagactcaac atggccttgg
      481 ccaggtccgc ttcgagacgg tcaaagaccg gctcgcccca cccgggcgga gcgccgcgtg
      541 cgatgccttc gacaatgccg ccgacggtgt caccggcccg gcgtgtcttc tcgatcagcc
      601 ggatcatgcg ctgcgccgca gccgggtcgg ggcagcggac gatattggac tcgatgtctt
      661 tggctttgac cttttccggg tccacggtag ccacgatgcg ctggacttgc cgcacatagg
      721 ccaggacttc gacgccgtaa tgccggcgca ggatcttctt ggcgatggcg ccggcggcga
      781 cccgcccgac ggtttcgcgc gcgctggtgc gcccgccgcc gggccaggcg cggatgccga
      841 acttggcctg gtacgtgtag tcggcgtgcg agggccggaa cttggtggcc atttcgttgt
      901 aggcctcgga ccgggcgtct tcgttcctga cccacaggca gatcggtgtg ccgagggtct
      961 tgccttcgaa ggtgcccgag agaatctgca cggtgtcact ctccttgcgc ggcgagacga
     1021 tcttcgactg gccgggccgg cggcgatcca aatccggctg gatgtcggcc tcggtcaacg
     1081 ggagccgggg cgggcagccg tccacgacca cgccgactcc gccaccatgc gattcccccc
     1141 aggtggtgat gcggaagaga tgcccaaacg tgtttgacat gtgcccagct tgcgggaaaa
     1201 gcgggcgccg gtcaaatgtg gaaaggggtg tgggaaaagg tgatgcgtga tgagcgagtg
     1261 gagccgattc aagtgcagca accctttggg ttgtgggccg gcagcgggcc atttcttcgc
     1321 ccaggagaca accggcggtg agcgggtgct acaccttacc tgtagtcacc agaccggcga
     1381 agcgctcgaa gctgcgatca taggtgcgct cgacgtcttt ggggaagcag caggcgggga
     1441 gctgtctcat gcccaggtgg tagtgcaggc actcgcagca gatgccgtgt ttttcgcagc
     1501 ccgggtaggt gcagttgcag aaccgcgcgt tgctcgactt cttgcattcc atagtgtggg
     1561 tttagtgaaa cgggaacccc accggctttg ccggggagac tcccagagtt tgacgtttcc
     1621 ggaagtcact taagatccgg ttcgcgcgcg gtaaaagagg taacggttag tgctcgagtg
     1681 cggatccatg atgtgaacaa ggccggcagg gctgttggtg gttagcaaag tttgccactg
     1741 gtttgtgcgc agggtgctgc tgtattcgac ggtcaaggtt tggccggccg gggaggttac
     1801 gtcgaaggcg aaggctcctc cgctccagat ggcgttcgtc agcgtcagtt ggtccccgga
     1861 tccggtggta aaggttccgc cactgtaccc acccagcggg gtgccctcca cgttccacaa
     1921 aatcgtccgg ttcgccggcc acgctgccgt cggcgtgcag gtcagcacag tgttgccggc
     1981 actccaggag agggatgtcg gcacatactc ataagggccc gaagcgagaa aagaggctgt
     2041 tgtcacgccg gtattcatgg cctcactgaa ggtaatgata accgctgccg agagcgcgac
     2101 accgctggca ccgctggcgg gaacggtcga gacgatggtg ggcgcagcgt aggcgcgccc
     2161 cagggacatg agcgaaaaca gcaaggcaaa aacccgcacc aggtaattgc caccaatcgg
     2221 caaagaaaag taattcataa tggtcttaat attactcgtt tcaggacttt gtgcatcact
     2281 aaatcgcagt tgccacccgc gagaacccgg aataccagaa agcttccaac ggcggcattg
     2341 actgcaagat caacggcgct tatgtgctgc ttggcctgct ctacggccac cgtgatccgg
     2401 acggcacgat ctccatcgcc atgcgcggcg gacaggattc cgactgcaac ccgtccagtg
     2461 cggcgggggt attgtttacc acaatgggct tcgcgaagct gcctgagcgg ttcaccaagg
     2521 acctgaacca gaccaacatc ttcagccaca cggcctacag cttcccgcgg ctgatcgccg
     2581 tctgcgagaa gctcgcgcgg gaggtcgtgg tgaagtccgg cggccggatc gaaaagcgga
     2641 gcggcgagga gttctttgtc ctgcccgtcc gggtgccgca gacgagcaag ctggagctga
     2701 gctgggcgcc cggcccgatt gccaatagcc gcttcacgcc tgcggagttg gcgcagatca
     2761 aggtgaacaa cgtccccgtc cacatgccaa ccaaccctcc ggctggtcct tcgaagcggc
     2821 ctattgggcc gagattgcag tggtgagtga gttaaccggg accgaccgcg ttactccgct
     2881 gcgctgtgtg agctgctccc tggctccctg gggagtgctc ttttcagctt ctcaatcagg
     2941 tgcggtgtca ggcatcttcc ctccgcacag atttatgtat cgggatcaaa gagtggtggt
     3001 ggtgatgccg gcctataatg cggcccggac gattcgccaa acctatgccg aagtgcggga
     3061 gcagcaggtg gtggatggca tcatcctggt tgacgacggc agccgggatg aaaccgtgtc
     3121 ggtcgcgcgg agcctcgagg gcgtgcaggt tcacgtgcac aaggtcaaca aaggctatgg
     3181 gggaaatcaa aagacctgtt accggctggc cctggaggcg ggggcggaca tcgtgatcat
     3241 gatccatccc gattaccagt acacgccgaa gttgattccg gcgatggtcg caatcatcgg
     3301 caacgggctg catccgtgtg tgctgggcag ccggattctg ggtggctacg ccttgcgcgg
     3361 gggcatgccc ctttggaaat acgtgtcaaa tcgctgcctg acgctagtgg agaatgtttt
     3421 gctcggcgcc aagttgtccg agtaccacac cggctatcgc
//
LOCUS       DATCGS010000320         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_424402, whole genome shotgun sequence.
ACCESSION   DATCGS010000320 DATCGS010000000
VERSION     DATCGS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424402"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VIL39_06510"
     CDS             <1..362
                     /locus_tag="VIL39_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEY5481115.1"
                     /translation="ESFQRDDGPPFFLLTVKAGGTGLNLTQAAHVIHFDRWWNPAVEN
                     QATDRAFRIGQKQNVLVHKFVCRGTIEERIDAVLEDKKALSAELLGGGGEKLLTEMSN
                     NELLRFVALDLSRATEA"
     gene            362..1213
                     /locus_tag="VIL39_06515"
     CDS             362..1213
                     /locus_tag="VIL39_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY5481116.1"
                     /translation="MRPRIMSWNHYGFRPYVSVAERRCKAAREMAKLAKKGKTIRPVQ
                     IEGRTIASTFWGKAWCDNLESYMDYENRLPRGRTYVRNGSVVHLDIRSGEIEATVSGS
                     ELYQVRVSIAPAAKAKWKRLCNDCSGAIGSLVELLRGRFSDHVMGIITRKETGLFPAP
                     SEIKLKCSCPDWATMCKHVAAVLYGVGARLDQSPELLFTLRSVNHEELITQAATATDL
                     AGKTASAGPELAESEIGAVFGIELDTQTATTKPAPAAPAPAKARRKARKPAPRKRRPK
                     AKRQKSR"
     gene            1470..3734
                     /locus_tag="VIL39_06520"
     CDS             1470..3734
                     /locus_tag="VIL39_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY5481117.1"
                     /translation="MDQDWLFGGKFDPAAIAPGFDDAAFTRVSLPHSVVKLSWQNWEP
                     AAWQEVWVYRRHFALPKECQGLRTFLHFDGVMVGATPTINGHTLPQHLGGYLPFRYEI
                     TNQLAEGGNVLAVAVDSRWSNVPPEGSPVGAKRVDYLEPGGIYRSVRLEAVPQIFIND
                     VFAKPVKVLDADRRVEVVCSIDAAAPASQPVQIEVELRSGDRVVSRAQETLRIEAAGP
                     KQVALTLSKLGNVALWDTRAPNLCDVVVTLSIGGKPVHDYRARIGLRDARFELDGFFL
                     NGRRLQLFGLNRHELYPYVGGAMPRRVMRRDAEMLRREFNCNIVRCSHYPQSEAFLDA
                     CDELGMMVWEETPGWGYIGDEAWKELLVRDVNDMILRDRNHPAIIIWGTRANESANDV
                     ELYRRTRALAKSLDDSRPTSGSMTPGSKKNWQRDWHEDVFAFDDYHSAPDGSVGIAAP
                     VPGVPYMLAEAVGQFNYSKGKGFDSKYRRAGDAITQQQQAVRHAQAHSKAAASPRNCG
                     VIAWCGFDYASLVNPHNNVKCPGVADVFRIPKLGAAFYQAQVSPQVRPVIQPSFYWDF
                     GPQTPRGPGKRAAIFSNCERLELFVNGRRQATLHPDSANFPHIQHPPFWADLELDGAG
                     HPELRIDGYVGGRLVLSRSFSSNAADDRFFLQADDTELIGDGADATRLAFKVVDKFGA
                     DRAFAGGEVAFDLTGPGVLVGDNPFSLVDSGGVGAIWIRTLPRRPGRITVKAVHSLLG
                     ARAVEIKVNGSART"
BASE COUNT          753 a         1237 c         1271 g          736 t
ORIGIN      
        1 tggaatcctt ccagcgggat gacggcccgc cattcttcct tttgacggtg aaagcgggcg
       61 gcacggggtt gaacctgact caagcggccc acgtcattca cttcgaccgc tggtggaatc
      121 cggccgtgga gaatcaggcc accgaccggg ccttccgcat cggccagaag caaaacgtgc
      181 tggtgcacaa gtttgtctgc cggggcacga tcgaggagcg cattgacgcg gtcctcgagg
      241 acaagaaggc gctgtcggct gaattgttgg gaggcggggg cgagaaactg ctcacggaaa
      301 tgagcaacaa tgaactgttg cgcttcgtag cgcttgattt gagccgggcg actgaggcat
      361 aatgcggcca cgcattatga gctggaacca ctacggattt cgaccttacg tatcggtggc
      421 tgaacgccgc tgcaaagcgg cgcgggaaat ggccaagctg gcaaagaagg gaaagaccat
      481 tcggcccgtc cagatcgagg gccgcacgat tgcgagcacc ttttggggca aggcctggtg
      541 cgacaacctg gagtcctaca tggactatga gaaccgcctg ccgcgcggcc ggacttacgt
      601 ccgcaacgga tcggtggtcc acctggacat ccgctccggc gagatcgagg cgacggttag
      661 cgggtcggag ctttaccagg tccgagtcag catcgccccg gcagccaagg cgaagtggaa
      721 gcggctttgc aacgactgct caggcgcgat tggctcgctg gtcgagttgc tgcgcgggcg
      781 gttctccgac catgtgatgg gcatcatcac gcgcaaggag accgggctct ttcccgcgcc
      841 ctcggagatc aagctcaagt gctcgtgccc ggattgggcg acgatgtgca aacatgttgc
      901 ggcggtccta tacggggtcg gcgcgcgcct ggaccaaagc ccggaactac tgttcacctt
      961 gcggtccgtc aaccacgagg aactgatcac ccaagctgcc accgcgaccg atctggcagg
     1021 caagaccgcg tccgccggtc ctgagctggc cgagagcgag atcggcgccg tgttcggcat
     1081 tgagttggat acccagaccg ccacgactaa gcccgcgcct gccgctccgg cgccagccaa
     1141 agctcggcgc aaagccagaa aacccgctcc gcgcaaacgc cgccccaagg ccaagcggca
     1201 aaagagccgg tgaaacgttt ggggcagccg agcgttcagt cgttgctgcg gccaattttg
     1261 gtttttgacg ttccgcccgg cattctgcac gctgcgcggg cagcatgagc aagagtcatc
     1321 aagaaacagg ccccgtttcc cgcagagagt tcgttcagca ttgtgccctc ggcgcgctgg
     1381 ccctggccgc agcccccatt ttgggatgcc gtagtgcccg ctccggtcgg acggtaccgc
     1441 gaaccggacc ggcaggccgc acaattccgt tggaccaaga ctggctcttc ggcgggaaat
     1501 tcgacccggc ggccatcgcc cccggctttg acgacgccgc gtttacacgg gtttcgctac
     1561 cacactccgt ggtcaagctt tcctggcaga attgggagcc ggcggcttgg caggaggttt
     1621 gggtttaccg ccggcacttt gcgctgccca aggagtgcca ggggttgcgc acgttcctgc
     1681 atttcgacgg ggtcatggtc ggagccacac cgaccatcaa cggccatacg ttgccacaac
     1741 acctgggcgg ctatcttccc ttccgctacg agattaccaa ccagctcgcc gagggcggca
     1801 acgtgctcgc ggtggcagtg gactcgcgtt ggagcaatgt gccgccggaa gggtctccgg
     1861 tcggggcgaa acgcgttgac tatctggaac cgggaggcat ctaccgctct gtccgcctgg
     1921 aggcggtgcc ccagatattc atcaacgacg tttttgccaa gccggtcaag gtgctggatg
     1981 ccgaccggcg ggtagaggtg gtctgctcaa ttgatgccgc cgccccagct tcccaacccg
     2041 tgcaaatcga ggttgagctg cggagcggcg atcgcgtcgt ctcccgcgcc caagaaacgt
     2101 tgcgcataga ggcagccggc ccgaagcaag tggcgctgac cttgtcgaag ctcggcaatg
     2161 tcgccttgtg ggatacgcgc gcgcccaatc tctgcgacgt cgtggtcacg ctctccatcg
     2221 ggggcaagcc cgtgcatgat taccgcgcgc gaatcggcct ccgggacgcg cggtttgaac
     2281 tcgacgggtt cttcctcaat ggccgccggc tgcagctctt tggtttgaac cgccatgaat
     2341 tgtatcccta tgttggcggg gccatgcccc gacgcgtcat gcgccgcgac gccgagatgc
     2401 ttcgccgcga gttcaactgc aacatcgtgc gctgctcgca ctatccgcag tccgaggcgt
     2461 tcctcgatgc ctgtgacgag ctgggcatga tggtgtggga ggaaaccccc ggctgggggt
     2521 atatcggtga cgaagcctgg aaagaactgc tggttcgcga tgtgaacgat atgatcctac
     2581 gcgaccgcaa ccaccccgcg atcatcatct ggggcacgcg cgccaacgaa tcggccaacg
     2641 acgtggaact ctatcggcgg acaagggcgc tcgcgaaatc cctcgacgac tcccgcccta
     2701 cctcgggctc gatgacgccg ggttccaaga agaactggca acgtgattgg cacgaagacg
     2761 tcttcgcctt cgacgattac cattcggctc ccgatggcag tgtgggcatc gctgccccgg
     2821 tgccgggagt gccctacatg ctggcggagg cggtgggcca attcaactac agcaagggca
     2881 agggttttga cagcaagtat cgccgcgccg gagatgcgat cacgcagcaa cagcaggccg
     2941 tgcggcacgc ccaggctcac agcaaagctg ccgccagccc gcgcaactgc ggggtgatcg
     3001 cctggtgcgg attcgactac gccagcttgg tcaatccgca taacaacgtg aaatgtcccg
     3061 gcgtagccga cgtgttccga atccctaaac tgggcgcagc gttttatcag gcgcaggtca
     3121 gtccccaagt gcgtccggtt atccagccca gcttctattg ggatttcgga ccgcaaacac
     3181 cgcgtgggcc gggaaagcgg gccgccatct tctccaactg cgagcggctc gaattgtttg
     3241 tcaatggccg gcgacaggcc acgctccacc cggactcagc gaacttccct cacatccagc
     3301 atccgccctt ttgggccgat cttgagctgg atggagccgg tcatcccgag ctgcggattg
     3361 acggatacgt cggcgggcgt cttgtgctgt cgcgctcgtt ttcctccaat gccgccgatg
     3421 atcgcttctt cctccaggcg gacgacacgg agttgatcgg tgacggcgca gacgcaacgc
     3481 ggctggcctt caaggtggtg gataagtttg gcgccgaccg ggcgttcgcc ggcggagaag
     3541 tcgcctttga tctcaccggg ccgggtgtgc tggtcgggga taacccgttt tcgctcgtgg
     3601 acagcggcgg cgttggcgca atctggatcc ggacgcttcc gcgccgtccc gggcgcatca
     3661 ccgtgaaagc ggtccattcc ctcttgggcg cgagggcggt cgaaatcaag gtcaacggtt
     3721 cggcgaggac ttgatattag aggtgtgccg gacttatccc aacccattac gtccgaggtg
     3781 gcaaccaatg cccaacggga tcgcagcggg ccaagctgag ccgaacgatc actgcgcgcc
     3841 gcgctgcgct aaaatcaaat cacgcactgc cttcgggtcc gggattgacc tgagttcctc
     3901 cgcggcgtca accgtggccg cagtggagag cgttagggtg ccgatcccga ccaggcgctg
     3961 gaaaaagctc tggtcaatgt caatggaccg aatgtct
//
LOCUS       DATCGS010000321         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_523938, whole genome shotgun sequence.
ACCESSION   DATCGS010000321 DATCGS010000000
VERSION     DATCGS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523938"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1144
                     /locus_tag="VIL39_06525"
     CDS             125..1144
                     /locus_tag="VIL39_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481118.1"
                     /translation="MFKINQQSLERMPVYTAKHIEAAMNKKPSRTPWPFRKAERNGET
                     FAVSGFTLIELLVVIAIIAILAALLLPALAKAKTKAQGIYCMNNTRQLMLANHMYQGD
                     NKDLFPMAFHGGFVPQPNGPQPWVSGWLTWDTSTDNTNTIYLLDSRYAVLAPYFGKAK
                     NIYKCPADVYASSAQIQRGWPSRCRSVSGDIYVGEGNAWTSGVGPPAYTASGPNNLTI
                     YKGAKKSPELQFPGPSGTWVYMDEHPDSINDAGAFAPNTASNIPDAPATYHNGAAGFA
                     MADGHSEIHKWKGPTMNKPRSAGGLKGVNFVAQNNFATVSTDPDLRWYSYATPRKSTK
                     IVTDP"
     gene            complement(1248..>2521)
                     /gene="miaB"
                     /locus_tag="VIL39_06530"
     CDS             complement(1248..>2521)
                     /gene="miaB"
                     /locus_tag="VIL39_06530"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY5481119.1"
                     /translation="AKGYSLAATEAAADVILLNTCSVRDLAEQKAIRKMENIAGQTRR
                     RRPNVVLGFMGCVAQARGSELIDRLPDVDLVVGTQKLHHTAEYLDDIFAGRRDKVVEV
                     AAEQGSEGAIREHLLNGNARKCGAAFVSIMQGCNQYCTFCIVPHTRGEERSRAISEVV
                     AECRELAARGVKEITLLGQIVTSYGRGDIPNKDGKSPFVQLLEAVDAIEGLERIRFTS
                     PHPKGYGDDLVGAYGRLGKLAENAHIPVQSGSDRVLKLMRRGYTRERFLGIIEKLRRV
                     KPDIGLSTDFIAGFPGETEEDFQQTLSLAREVGFDQAFVFKYSQRRNTPAADMPDPVS
                     QAVKEDRNQRLLEVVNVQGARQHQKFVGRQVQILVEGPSKRNPARMTGRTRCNKIVVF
                     DASPRHCGQLLDVKIQRAGNFTLYGEADDQR"
BASE COUNT          509 a          807 c          686 g          519 t
ORIGIN      
        1 tggggagttg tgcgggctga ggagagagcc tcatttcttc agccgaaacg gacctcacta
       61 ttttcccttg ccaggacagt cgaaagtgct acgatttgag tagtgcaagt cattggttta
      121 aattatgttc aagattaacc aacagtcttt ggagagaatg ccggtttata ctgcgaaaca
      181 catcgaggct gctatgaata aaaaaccatc acggacaccc tggcccttcc gcaaggccga
      241 aagaaacggc gagacttttg ccgtctcagg tttcaccttg atcgaactgc tggtggtcat
      301 cgccatcatt gccatcctgg cggcgctgct cttgccggcc ctggccaaag ccaagaccaa
      361 ggcgcagggc atttattgca tgaacaacac caggcagttg atgctggcca atcatatgta
      421 ccagggcgac aacaaggatc tctttcccat ggcctttcat ggcgggttcg tgccgcaacc
      481 gaatggtcct caaccgtggg tgagcggatg gttgacttgg gatactagca cggacaacac
      541 gaataccatc tatctcctgg attctcggta cgcagtcctg gcaccctatt tcggcaaagc
      601 gaaaaacatt tataagtgcc ctgcggacgt ttacgccagc tcggcacaga tccaacgagg
      661 ctggccatcg cggtgccgca gtgtttccgg cgacatttac gtcggagaag gcaatgcttg
      721 gacgtctggt gttggccctc ctgcctatac ggccagcggc ccgaacaacc tgaccatcta
      781 caagggtgcc aagaagtctc ccgaactgca attccccggc ccctctggga cgtgggtata
      841 tatggacgaa catcctgaca gcatcaacga tgctggcgcc tttgccccaa acactgccag
      901 caacattccg gacgcgcccg ccacgtatca taacggtgcg gctggctttg ccatggccga
      961 tgggcactct gaaatccaca aatggaaggg cccaacgatg aacaagccca gaagcgctgg
     1021 cggattaaag ggcgtcaact ttgtggcgca aaacaacttc gcaaccgttt ccactgaccc
     1081 tgacctgcgt tggtactcgt acgctacccc ccgtaagtca actaagatcg tcaccgaccc
     1141 ataggtattg tggaagaccc aagggcaggc catttctggg gtctccccgt ctttgagcgt
     1201 ggggttggcc tccgtccccc tgttgcgcag ttgacaatgc gctgacctca gcgctggtcg
     1261 tccgcttccc cgtagagcgt gaagttgccg gcgcgctgga tcttcacgtc cagcaactgg
     1321 ccgcagtgcc ggggcgaagc atcgaacacg acgattttgt tgcagcgcgt ccggcccgtc
     1381 atgcgggcgg gattcctctt gcttggcccc tccacgagaa tctgaacctg ccggcccacg
     1441 aacttctggt gctgccgtgc accctggaca ttcaccactt cgagcagccg ctggttccgg
     1501 tcttccttca ccgcctgact caccgggtcg ggcatgtccg ccgctggtgt gttgcgccgc
     1561 tgggaatact taaaaacgaa tgcctggtca aatccgactt cgcgtgccag cgatagcgtt
     1621 tgctgaaaat cctcttccgt ctcaccagga aaaccggcga tgaaatccgt gctaaggccg
     1681 atatccggct tcacgcggcg cagcttctca ataatgccca ggaaccgctc ccgcgtgtaa
     1741 ccgcggcgca tcagcttgag cacccggtcg ctgccgctct gcacggggat gtgcgcgttt
     1801 tccgccagct tgcccagccg cccgtaggcg cccaccaggt catccccgta gcctttcggg
     1861 tggggtgagg tgaaccggat acgttccaac ccctcaatgg catccaccgc ttcgagcagt
     1921 tgcacgaaag gggacttccc gtccttattg ggaatatcgc cccggccgta gctggtgacg
     1981 atctgtccca gcagcgtgat ctccttcacg ccgcgcgcgg ccagttcccg gcactcggcc
     2041 acaacctcgg aaatcgcccg gctgcgctcc tccccacggg tgtggggcac gatgcagaac
     2101 gtgcaatact ggttgcagcc ctgcatgatg ctcacgaacg ccgcaccgca tttcctggcg
     2161 ttcccgttga gcaaatgctc gcgaatcgcg ccctcgctcc cctgctctgc cgcgacctca
     2221 accaccttat cgcgccggcc ggcgaaaatg tcgtcgagat actccgcggt gtgatgcagc
     2281 ttctgcgtcc ccacaaccag gtccacgtcc ggcaaccggt caatcaactc gctgccccgg
     2341 gcctgggcga cgcagcccat gaaaccgagc acgacattgg gccggcggcg gcgcgtctgg
     2401 cccgcaatgt tctccatctt ccgaatcgcc ttctgctcgg ccaaatcccg cacgctgcac
     2461 gtgttaagca ggatgacatc ggcggcagcc tccgtcgccg caagcgaata gcccttggcg
     2521 a
//
LOCUS       DATCGS010000322         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_721548, whole genome shotgun sequence.
ACCESSION   DATCGS010000322 DATCGS010000000
VERSION     DATCGS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721548"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIL39_06535"
     CDS             complement(<1..195)
                     /locus_tag="VIL39_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481120.1"
                     /translation="MNTLAIEAEVDNEGWLNIHAPAPPGTAPGKLDVVVVWSPLTPAA
                     APRLRARAGTLSGKVELAADF"
     gene            324..557
                     /locus_tag="VIL39_06540"
     CDS             324..557
                     /locus_tag="VIL39_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481121.1"
                     /translation="MAVITLDRVLTEAESLPADEQVMLEDLLRQRRLESWRRETADDA
                     RQAAEDFRTGKLKSQSADDVIARLRAGLNADAG"
     gene            662..844
                     /locus_tag="VIL39_06545"
     CDS             662..844
                     /locus_tag="VIL39_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEY5481122.1"
                     /translation="MAANLADPRLKAHHLSGPLAGLHACSAAYDCRIVFAKQKHPQTG
                     TEVLLLVNIGSHDEVY"
     gene            complement(1001..1393)
                     /locus_tag="VIL39_06550"
     CDS             complement(1001..1393)
                     /locus_tag="VIL39_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5481123.1"
                     /translation="MKTYWDSSALVEALHAPGLRSRIRRGENGTRPHSLAEVFSTLTK
                     GVNFRYPPADAANMAADLAKDLDFVELSAADAVSALKKASSLGVRGARIHDLMHAEAA
                     IKYGADELLTRDDAGFASLNLPLQVAAP"
     gene            complement(1390..2013)
                     /locus_tag="VIL39_06555"
     CDS             complement(1390..2013)
                     /locus_tag="VIL39_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481124.1"
                     /translation="MLFALIHGGPILPELVEGGNRQVLPVFWRFCPIAKTVGGRQRPS
                     GQAMREKQRDSGANRAGGGQCRAEARLQTGLENNGSQSAIRRRLQARGFGVEAAWGGD
                     GAGVIVAPPEGLEKGLETRPQQWDYSIMKLVADDNGRLACKSLFTPKRAFSAERQADG
                     SIRLVELVEKEVPVVRARKVNGRWMGAEMQLDRAKVIRAIRADREAR"
     gene            2130..>2582
                     /locus_tag="VIL39_06560"
     CDS             2130..>2582
                     /locus_tag="VIL39_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEY5481125.1"
                     /translation="MRHQRTLIAASIVGIALAAAVTASAAGPPELSPKEQLGKALFFD
                     TNLSTPPGMACAACHGPEVGFTGPDAAFNAAGAVYEGAVSGRFGNRKPPTSAYAGDSP
                     VFQLDAITGNFVGGMFWDGRATGWTLGDPLAEQAQGPFLNPLEQNNASP"
BASE COUNT          509 a          858 c          763 g          452 t
ORIGIN      
        1 gaaatcggcg gccagttcga ccttgccaga cagggtgccg gcacgggccc ggaggcgcgg
       61 ggcggcggct ggcgttagcg gggaccagac caccaccacg tccagcttac ccggagcggt
      121 gccgggcggt gcgggggcat ggatgttcaa ccaaccttcg ttgtccactt cggcttcaat
      181 ggccaacgta ttcatagggc aatatgtagc ccaagacggc gcttggcaaa gggaaagccg
      241 cccccagtct gaaggcagtg gccgaaaagc ttgacgcccc ggaccaacca gcgtaaagac
      301 tctctgcgaa cggaaatcaa tctatggctg taataacctt ggaccgagtg ttgacggaag
      361 ccgaatcctt gccggcggac gaacaggtga tgctggagga tttactccgc cagcgccgcc
      421 tcgaatcgtg gcggcgcgaa acagcggacg atgccaggca agccgccgag gacttccgca
      481 ccggcaaact caagagccag tcggctgatg atgtcatcgc ccgcctgcgc gccggattga
      541 acgccgacgc cggttgacga tgcacgaact ggttcccacg ccgaagttcg agcgggcgtt
      601 tcagcgtctt gtgcgcaaga accccgcgct ccagccccag attgaataga ccctgcggcg
      661 gatggccgcg aacctggccg atccgcgctt gaaagcccac cacctgagcg gcccactggc
      721 tggcctccat gcctgttccg ccgcctacga ctgccggatc gtcttcgcca aacagaaaca
      781 tccccagacc gggacggagg ttctcctcct ggtcaacatc ggctcccatg acgaagtgta
      841 ttgagctaac ggccgatctc cccgtcgcac cgctcttggc cggcgacacc gcccaagtcc
      901 tggcggcatt gacggggcac gaaccgctaa acacgaaatc tccggaaacc agtgccaaaa
      961 gtgaggattg acgcttgctc gaggcagtcg agcgcgtgtt ttagggcgct gcaacttgca
     1021 gggggaggtt caggctcgca aatccagcat cgtcccgcgt tagcaattca tcagccccgt
     1081 acttgatggc cgcctcggcg tgcatgaggt cgtgaatccg ggcgccgcgc accccgagcg
     1141 agctggcttt cttgagcgcg gacactgcgt cggcagcaga cagctcgacg aaatccaggt
     1201 ctttggccag atcggctgcc atgttggcag cgtctgccgg cggataccga aagttgaccc
     1261 ctttggtgag cgtcgaaaag acttccgcca ggctatgcgg gcgggtcccg ttttctccac
     1321 ggcgaatccg ggagcgcaac ccgggagcgt gcagggcctc gactaacgcc gaggagtccc
     1381 aataggtctt catcgggcct ctcggtcggc tctaatcgcg cggatcacct tggcacggtc
     1441 gagctgcatc tcggccccca tccagcgacc gttcactttg cgcgcgcgaa ccaccgggac
     1501 ctctttctcc accagttcaa ccagccggat actgccatcg gcctggcgtt cggcggaaaa
     1561 cgctctcttc ggtgtgaaaa ggcttttgca ggccagccgc ccattgtcat cagccaccag
     1621 tttcataatg gaataatccc attgctgcgg ccgcgtttca agccctttct ccagaccctc
     1681 gggcggcgcg acgattacac ccgcaccatc gcctccccac gcggcctcga cgccaaaacc
     1741 tcgagcctgg agtcgccggc ggatagccga ctgagaaccg ttgttttcca ggccggtttg
     1801 aaggcgggct tctgcccggc actggcctcc tccagcccgg tttgccccgg aatcgcgctg
     1861 cttctcgcgc attgcctggc cgctcggccg ctgccgcccg cctacggtct tggcgatggg
     1921 gcaaaaccgc cagaaaaccg gcaaaacttg cctgttgccg ccttccacca gctctggcag
     1981 gatggggccg ccatgaatga gagcgaaaag caattctcct tcacccgcgc agcgtcaatg
     2041 aaggagcggc gcggcaaaaa gcaacttcct atcagttcag tgggcagtaa acatcaacga
     2101 cggcaaacta gagaaagaaa ggtatcaaga tgagacacca acgaacacta attgcagcat
     2161 ccattgtcgg tatcgcgctg gccgctgccg tgactgcatc ggcggcaggc cctccggaac
     2221 tcagtcccaa agaacaactg ggcaaggccc ttttcttcga taccaacctc tccacgcccc
     2281 ccggcatggc gtgcgccgcg tgtcacggtc cggaggtcgg cttcaccggc cccgatgcgg
     2341 cgttcaacgc cgccggggct gtctatgagg gggcggtttc aggccggttt ggcaaccgca
     2401 agcccccgac ctccgcctac gcgggtgaca gcccggtttt ccagctcgac gccatcaccg
     2461 gcaacttcgt cggcggcatg ttctgggacg ggcgcgccac tggctggact ctcggcgacc
     2521 cgctggccga acaggcgcaa ggtccgttcc tcaacccgct cgaacagaac aatgccagtc
     2581 cc
//
LOCUS       DATCGS010000323         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_128076, whole genome shotgun sequence.
ACCESSION   DATCGS010000323 DATCGS010000000
VERSION     DATCGS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128076"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1542
                     /locus_tag="VIL39_06565"
     CDS             433..1542
                     /locus_tag="VIL39_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481126.1"
                     /translation="MKKFPEPIDLRHVKVYPLSERQSLSALDQILVDPSQPPRACDPA
                     MLATIRDCAAKVAAARQRESSVILMYGAHLIKNGAMGIVNELITRGWVTHLATNGAGT
                     IHDWELSFLGRTEESVKKNVITGTFGTWDETGRSINLALLAGALQHEGYGRSLGRFIC
                     EDGVTLPATGLLDKSLRDRPADPLAPARAELLQAMLTHRLPAGRIAVKHPWKETSILA
                     QAFRQNVPMTVHPGIGYDIITNHPMFSGAAIGRAAAMDFGLFGRAVEGLDGGVVLSVG
                     SAIMAPQVFEKSMSGVNNLRLQAGRRIVHDHTIYVVDIQDGGHWDWSKGEPPKDNPAY
                     YLRFCKSFSRMGGQMNYVQCDNVAFLHNLLKLLPQ"
     gene            1548..2579
                     /locus_tag="VIL39_06570"
     CDS             1548..2579
                     /locus_tag="VIL39_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5481127.1"
                     /translation="MDSARFNAIVERYCDLSIAVLGDFCLDRYLEIDPAKQEISLETG
                     LPVRNVVNVRAQPGGAGTIVNNLAALGVGNIFPVGFAGDDGEGFELWGALERLPGVRL
                     GHFVQTAQRHTFTYCKPLVIAPDKPPVELNRLDLKNWTPTSALLQGLLASRLKTVAEL
                     VSAVIVLDQVDVPETGVVTRKVREAAKEVAEQDPELLILADSRRGLRGFPPVTFKMNA
                     AELCALTGARGELSLEQIKEAADALARRNGRSVFVTLAERGILGAAGSGQVEHVPALP
                     VRGAIDIVGAGDAVTANLVAALAAGAALREALEIANAAASVVVHKLGTTGTASVAEIR
                     ELLVPPGSR"
     gene            2582..3472
                     /locus_tag="VIL39_06575"
     CDS             2582..3472
                     /locus_tag="VIL39_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481128.1"
                     /translation="MARPIARLLTGFFAVAAVLLVAALILTLSQPAPAPPPLPMPNGY
                     DDFVKAGGMVTDTASDYGTMSEEELRAFVKKNAEALKLVRTGLGRECRVPLDYSATNT
                     AFLGNLAAIKRLAQAMTAEGRLAEMENRPADAAAAYLAVMRLGVASSHGGLIINSLVS
                     IAIDTIGAVRMEKLVTNLDAKQCREAAAALESCEAQREPTDTIMRQERAWARRVYGLK
                     GQIARLIRYKSLKQSEGKWVAKAKAQQTRTRSLLIQLAAHAYELEKGQRPKSLADLVP
                     VYLKTIPQNPLTGTNMAYYP"
     gene            3591..>3773
                     /locus_tag="VIL39_06580"
     CDS             3591..>3773
                     /locus_tag="VIL39_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481129.1"
                     /translation="MNRKLREARTLAFTLIELLVVIAIIAILAALLLPALANAKEKAK
                     RTACKSNMRQAILSVHM"
BASE COUNT          747 a         1130 c         1180 g          716 t
ORIGIN      
        1 agccagtaga tttgggggtg ctcccgtatg catcccttga ttatcccttg attatccctt
       61 gattatccgc cggccgttcc aggggcaaat tcgggaacgc tacgctgtct ccagggtgtc
      121 ccagcatcat tttgacagca ctctctggtg cgctattcaa atgagactgc atttttccgc
      181 gaaccggttc ctccctgctt tcgttcaccc tggagcggcc attccagggg cttcaccctg
      241 cttgccgaca gtgtcgaagg agaaccgata aaggctggcg gcggtcgcac gattgacttc
      301 gcaggaggag cgggttttac tcgtgggcaa atgtgtttcg tggcgccgtc cggcagtttg
      361 atgaaactca acgattgatc tgatcctgag cgagtgagcc ccagaccaaa gcctggcgtg
      421 gcgcggctac ctatgaagaa gttccctgaa cccattgacc tgaggcacgt gaaggtttac
      481 ccgctgagcg agcggcaaag cttgagcgcg ctcgaccaaa tactggttga tcccagtcag
      541 ccgccccgcg cctgcgatcc tgccatgctg gcgaccatcc gcgactgcgc cgcgaaagtt
      601 gccgccgccc gccagcggga gtccagcgtg atcctgatgt atggcgcgca cctgatcaag
      661 aacggcgcta tggggattgt gaatgaattg ataacccgcg gctgggtgac acatcttgcc
      721 accaatggag cgggcaccat tcacgattgg gagctgtcgt ttctcggccg caccgaagaa
      781 agcgtgaaga agaatgtgat cacggggact tttggcacgt gggatgaaac cgggcgcagc
      841 attaatctgg cgctgctggc tggggcgctg cagcacgaag gttacggccg cagcctgggc
      901 cggtttattt gcgaggacgg agtgaccctg cctgcaaccg ggctgttgga caagtcgctg
      961 cgcgaccgtc cggctgaccc gctggcgccg gcccgggcgg aactgctgca agccatgctc
     1021 acccaccggc ttccggccgg gcgaatcgcc gtgaagcacc cgtggaagga gacttcaatc
     1081 ctggcgcagg cgtttcggca gaatgttccg atgaccgtcc atcctggcat cggttacgac
     1141 atcatcacga atcatccgat gttcagtggg gcggcgattg gccgcgcggc ggctatggac
     1201 tttggcctat tcggacgcgc ggtggaaggg ttggacggag gcgtggtctt gtcggtgggt
     1261 tcggccatca tggcgccgca agtgtttgag aagagcatga gtggcgtcaa caatctgcgc
     1321 ttgcaggccg gccggagaat tgtccacgac cacaccatct atgtcgtgga catccaggac
     1381 ggcggccatt gggattggtc aaagggcgag ccgcccaagg acaacccggc ctactacctg
     1441 cgtttctgca agagcttctc ccgcatgggc ggccagatga actacgtgca atgcgataac
     1501 gtcgcattcc ttcataacct gctgaaactg cttccgcagt aaaacgcatg gactccgcgc
     1561 gcttcaacgc tattgtcgag cgctactgcg atctgagcat cgccgtcctg ggcgactttt
     1621 gcctcgaccg ctacctggaa atagatccgg cgaagcagga aatctcgctc gaaaccggcc
     1681 tgcccgtccg caacgtcgta aacgtgcgcg ctcagccagg cggcgcgggc acgattgtga
     1741 ataatctggc cgcgctgggt gtggggaaca tctttccagt cggctttgcc ggtgacgacg
     1801 gagaagggtt tgagttgtgg ggcgccctcg aaaggcttcc cggcgttcgg ctgggccatt
     1861 tcgtgcagac cgcccagcgg cacacgttca cctactgcaa accgctggtt atcgccccgg
     1921 acaaaccacc ggtggaactc aaccgactcg acctcaagaa ctggaccccg acctcggccc
     1981 tgctccaggg cctcctggca agccggctaa agactgtggc agaactggtc agtgcggtga
     2041 ttgtcctgga ccaggttgac gtgccggaaa ccggcgtggt gacgcgcaaa gtgcgcgagg
     2101 ccgcgaaaga agtggcggag caagaccccg aactgttgat cctggcagac agccgccggg
     2161 ggttacgcgg ctttccgccg gtgaccttca agatgaatgc ggctgaacta tgtgccctca
     2221 cgggggccag gggcgaattg agtctggagc agattaagga agccgccgat gcgctcgcgc
     2281 gccggaacgg gcggagtgtc tttgttacgc ttgccgaacg cggcattctc ggcgctgcgg
     2341 gcagcggcca ggtcgagcat gtgcccgccc tgccagttcg cggggcaatt gacattgtgg
     2401 gcgccggcga cgccgtgacg gctaatcttg ttgcggcgct tgcggccgga gcggccttgc
     2461 gcgaggccct ggaaatcgcc aacgcagccg cctcggtagt cgtccacaaa cttggcacca
     2521 cgggcacggc gagcgttgcg gaaatccggg agctgctcgt cccgcccggc agccgctgat
     2581 cgtggcgcga ccgattgcca ggctgttgac cggcttcttc gctgttgcgg cagtgctgct
     2641 cgtcgccgcg ctgattctga ccttgagcca gcccgcgccc gcgccgccgc ccttgccgat
     2701 gccaaacggg tatgacgact tcgtcaaggc cggggggatg gtcacagaca ctgcttccga
     2761 ctacggcacg atgagcgagg aggaactgcg ggcgttcgtg aagaagaatg ccgaggcgct
     2821 gaagttggtg aggacaggtc tgggccgcga gtgtcgtgtg ccgctggatt actccgcaac
     2881 caacacagcc tttttgggta acctggctgc catcaaacgg ctcgcccagg ccatgacggc
     2941 ggaaggccgg cttgcggaaa tggagaaccg cccagccgac gccgcggcag cttatctggc
     3001 agtaatgcgt ctgggtgtgg cgagcagcca cggcgggctg atcatcaact cactggtgag
     3061 cattgctatt gacacaattg gcgccgtccg catggagaag ctggtcacga acctggatgc
     3121 caaacaatgc cgtgaagccg ccgcagccct cgaatcctgc gaggcgcagc gcgagccaac
     3181 ggataccatt atgaggcagg aaagggcatg ggcgcgtcga gtctatggcc tcaaggggca
     3241 gattgcccga ctgatcaggt acaagtcctt gaagcagagt gaggggaaat gggtggctaa
     3301 ggcaaaggcc cagcaaaccc ggacccgatc gctgctcatc caacttgccg cgcatgccta
     3361 cgaattggag aaaggccagc gcccgaagag cctggccgat ttggtcccgg tgtatctcaa
     3421 gaccatcccc cagaacccgc tgactgggac caacatggcc tactacccgt gaaccttgag
     3481 caaaggagta tcccagagac aaacctcacc cgacttttct cattgccgca cgactggccg
     3541 aggcggatac ttataccgtg cccgaaagaa agcctctcat gcaccccttt ttgaatcgca
     3601 agctgcgcga agccagaacc ctcgcgttca ctctcatcga gctgctggtg gttattgcca
     3661 tcattgccat cctcgccgcg ttgctgctgc cggcgctagc caatgccaaa gagaaagcca
     3721 agcgcacggc ctgcaagagc aacatgcgcc aggccatcct ttcggttcac atg
//
LOCUS       DATCGS010000324        12555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_128112, whole genome shotgun sequence.
ACCESSION   DATCGS010000324 DATCGS010000000
VERSION     DATCGS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12555
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128112"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..336
                     /locus_tag="VIL39_06585"
                     /pseudo
     CDS             226..336
                     /locus_tag="VIL39_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007303424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(3420..3914)
                     /locus_tag="VIL39_06590"
     CDS             complement(3420..3914)
                     /locus_tag="VIL39_06590"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY5481130.1"
                     /translation="MMDLSGALAAVVDSVGWSADDWRMSEMIYPRSPREVMGGWMHLP
                     RFIDKVRLHLAGKLHPDYQPNLCKGFDGLWLETAGVEAKQFIEVVRHSLTDGEVCDWV
                     LKNVKQPDSVKAAHRARMLDYPRKDDAEMQARLKLRKEQAGLAQREDIQCFVDFIDAD
                     EKRI"
     gene            complement(3938..5758)
                     /locus_tag="VIL39_06595"
     CDS             complement(3938..5758)
                     /locus_tag="VIL39_06595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5481131.1"
                     /translation="MPFLHEVLHKFEVGGTMRFLRAGLTVLAVVLLTGLYDWRAFRNM
                     ATQEAMDMAQLGRNIAQGKGYTTLFIRPFSIHLVKARNMEKESMPQIGKTKDLAELRG
                     MHPDVSNPPVYPVALAALMKVLPFNYTISSPKSFSRFKPDFLIALFNQLLFLAVIALV
                     FLLARRLFDPGVAWLSAGLLLGTELFWRFSVSGLSTMLLLLIFMGLAWCLVLLEEQIR
                     EPKWGPYGVLVLAGLTGAMVGLGGLTRYSFGWLIIPVIVFLILFSGRRRTVLTLIVLA
                     AFALVMGPWIVRNYNVSGSPFGTATYSVVETTMFYPENRLQRSLEPDFSRLYVIAFWL
                     KLNTNLRQVITDELPRLGGSWVAAFFLVGLMIGFRSPALTRLRYFLVGCIVVLVLAQA
                     LGRTQLSEESHEINSENLLVLVAPLVLIYGVSLFFVLIEQIPMVFPQLRYLVVGLFSV
                     VACLPMLFVFLPPSTRPVVYPPYMPPAIQTVAGWLKEKELVMSDIPWAVAWYGQRQCV
                     WLTLKCRPDVTDSTAREDFFAINDYMKPINALYLTPQTMDARFLTQWAQAGDLSWGFF
                     IMDSMVKKRVPYDFPLNQMPDGWGREQLVLTDWQRWRKSP"
     gene            complement(5761..7134)
                     /locus_tag="VIL39_06600"
     CDS             complement(5761..7134)
                     /locus_tag="VIL39_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5481132.1"
                     /translation="MTPTLLIVDDEKTTREGLRAALEPHYDVYLAEDAKAAVELLEKD
                     HFDVLLTDFRLPTEDGMKLITRAKSLSKPPICILMTAYGSEELAVEAMKRGADDYIAK
                     GRMQIDELEVRIANALRRQNLEVENVSLHQQLDAKFGAQNLVGESRAMKEVLEVVQQV
                     APTRATVLLLGETGTGKEMVAKVIHQLSPRAQQPMVTVHCAALAPTLLESELFGHEKG
                     AFTGAYERRIGRFEQAQGGTLFLDEIGEIDASTQVKLLRFLGERTFERVGSNKTQSAD
                     VRLIAATNKDLEEQVKTGAFREDLFFRLRVVEIRLPALRERLEDIPLLAREFLREFSQ
                     ENSKPVESFTADAFELLMHYQWPGNVRELRTAIEHAVVLCRGDKVTVRDLPTSLREAV
                     GRAPAEKDPKGQVVRSDLTAQEAERELIIRALKATNGHRTLAAKRLGMSRRTFHRRLH
                     LHHLEEI"
     gene            7272..7883
                     /locus_tag="VIL39_06605"
     CDS             7272..7883
                     /locus_tag="VIL39_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY5481133.1"
                     /translation="MNGKWSKWLPYVFMLVAALSRWPKLFPPNFSAFYGLAFCAGAFF
                     PRNVKWWLPLGTLVVTDIALDFYYQSFSAMQLVNYAAYALLILFGTRFSHRSRFLNLL
                     AGGIMGAILFYLITNTASWLSNPEYAKDLAGWIKALTTGTAGYPTTLEFFRNTLMSGG
                     LFTGLFAGALKLTEAAEPVKEKEPEEEPAQAPEGEEVPDEGKA"
     gene            7918..8460
                     /locus_tag="VIL39_06610"
     CDS             7918..8460
                     /locus_tag="VIL39_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY5481134.1"
                     /translation="MRDGNKSPHPVLLASFTNHASRIMLKIGVISDTHGYFDPRIPHL
                     FAGVDHILHAGDIGLPTIILALEQIAPVTAVAGNTDDPGFHYRLTEVVDLASRKFLVH
                     HIVNPQSLGDPIKTRIARERPDVIVFGHTHKPFSETIGGTRFFNPGYAGKSRFGLERS
                     VAILRCSEKGIRTEYLPLQS"
     gene            complement(8559..10361)
                     /locus_tag="VIL39_06615"
     CDS             complement(8559..10361)
                     /locus_tag="VIL39_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481135.1"
                     /translation="MKTNSPTSSLFTQRIVSSGVLPIGLGILVIVMTGQISCMASKLG
                     SVHVTSARFAGELILLDPGPVGVITVERPAMCSYDPPRGQINRAADGAGDAARAALEM
                     TTPENPEANIIVSAAGFVAAAPAAVIGGVLASRSRLPQDKLAECEAGLAQALTNMSGQ
                     HHLRDRILDAAKAAGRRRFVALPASDVPAQQRENHELTATPPVGTILETRADELRLER
                     KGAGDSSFALRIKARVRLLRSSTGEVISDETFEYQSGQDLFLDWALNNGEPFMKCTDF
                     GYRRLADQIVERMFEVTGEATILVGVGAAKPSVHTPRPQVRLADHSPAPALAPQVQLV
                     SQVVASPGTLYVYSPSRGDFMSIQRPLTKDEAASEAQSDVNEMMDGLITHPNMFVSLT
                     AIAAAIPVSIYLQTAGGIRGLSDKKYSAANTQVTTAARSSRPADDLARVIAQTLSQRC
                     SGMVVLLDKPRDGGEQVALVQPTTGEPILVGWHGDGTTNPRAGDKALRIEVLSAALKG
                     NESVNPSLAVHLEAQATLLRGDDGREIYSCPVHYRGRAHKFTAWAAQDAKLFREELQR
                     CYGELSGTVIDQMIARHLIAPGENPNLLLADDKK"
     gene            10411..11376
                     /locus_tag="VIL39_06620"
     CDS             10411..11376
                     /locus_tag="VIL39_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5481136.1"
                     /translation="MAAFGLANSVNIHHLELFYYVARHGGISEAMRHMPYGIQQPAIS
                     DQVIQLEESLGVTLFQRRPFSLTPAGKELYESIMPFFDSLTPMADRLRGGVSQHVRIA
                     ASETVLRDHLPAALQELRKQFPKLKVTLREGYDPEVISWLEQQEIDVAIGFLGSKPPT
                     GIHALPITRLPLILLVPRSSKLKSADELWQRDRIEESLITVPSNEPMCRAFQEGLGKL
                     KVDWFSGIGVSTLEMVQTYVANGYGIGVTVGIPQAKYHPQVRVLRLEGFPMPVFGVLW
                     QGRPTPVVSGLIEIVRQATVELLRGEDPALLLPGSELAMAGQGRG"
     gene            11499..11687
                     /locus_tag="VIL39_06625"
     CDS             11499..11687
                     /locus_tag="VIL39_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481137.1"
                     /translation="MTVRQFYDWQTAGGGDVSLLVETPERRASKRQKDLLDTMRLVEA
                     HPKLRKLLPADLAKKIKG"
     gene            11684..>12555
                     /locus_tag="VIL39_06630"
     CDS             11684..>12555
                     /locus_tag="VIL39_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481138.1"
                     /translation="MIHRHLNHQELSLAAIDDVIARGKRKDWEELRLAVLASPAFLEK
                     IQQVCRAHSANVNCDKQHISLRRGFTLIELLVVIAIIAILAALLLPALARAKATAKTA
                     QCVSNLHQMGLALIMYADENDGLAARANGPHWWQVLAPNLGGQTGANSTKVRLYTCPS
                     YPDPDPHYPGQKQLVCYVVNGWKFASPTDPTGTELTGLSKITMIQRPVDTIYLADRED
                     GTDYGPITTADPNTYSDYYDVWEPSHLPYLANGTASPRNGLSNNARRVAISRHTKSDA
                     LLYFDTHAATKKTR"
BASE COUNT         2436 a         3922 c         3808 g         2389 t
ORIGIN      
        1 gtggttgggt tcatgccgcg cgccatgcgc gtggaccgct tgctgggcga gcacggcatt
       61 gggcaggact ccgcagcggg gcgtcaggag ttcgagcggc agatggaaag gcgccggctg
      121 gaggccctcg acgaggaagc gctcaagccg ctgcggcggg gctggtgctt gggccgcgag
      181 cagttcaaag agcagatgct gatggagggt aagcttgatg agaacatgcg gcaggtcctg
      241 attcatcccg gcgaggacgg ctactgggtg gcggagtgcc cgagcctgcc cgggtgcgtg
      301 tcccgaggcg ccacgcgtga ggaatcggtc cgggacaatc cattggcgca gaccgttgtc
      361 cccgcgcatc gagaactgga ccggggcacc ttgcgctgct ttactccaat atgctcgcaa
      421 gctgcgaagg ttcttgtgga tgagctgcca cgcgaagcgc atggagtgcg tccagcttgc
      481 tggcgctgtt gtaatgcgtg gggcggtctg aaagcgggag caagctccac gcactccaaa
      541 cgcttcgcgt cgcagctcat caatgaaaac tcccgcagct tacgaagagc ttcgactgcc
      601 gcaatccaca ccgcgccgcg agcggcaacg ggttttgggc ttgggatctg gtgtttgatc
      661 ggcctgctcg tggggctggg tgcggcgggc ctcgtgtccg cggcgccgtg gtcggacccg
      721 gcggtggaca gctacaacat gcgtgtgggc acgcagacgt tttcagggct ctacaagttc
      781 accaccaaca cgttgctggt ggagacggct gaggcgatca ccaacctggg gagcgacacg
      841 atcaagttct acatggggca caacacttcc gggcaaagcg gggtcacgct tacgtccaac
      901 attacgaacc tgctgacgct ggcgcgggat gagccgtcct accgccgggt gctggacatg
      961 ccattccggc gcctcatcat ctgggaatac ccgctctcca atggcgatgc gccgtttcag
     1021 gatggcaatt acaccagcac cgaacaggcc aacgattacc gggaaatgta cgacctgact
     1081 cgttacttcc taaccaacta caacggctcg gccaagacct tctatcttgg gcattgggag
     1141 ggcgacgggt acctgcgcgt caacaactgg tccaccaatc ccagcgcggc ggtggtctcg
     1201 gcgatgattc tctgggaaaa tacccgccag aaggcggtgg atgacgccaa agcaagcacc
     1261 agctacagca acgtgaacgt cttctactac gccgaggcca accgggtgcg ggacgccatg
     1321 ctcaacggca cgaacaacaa cgtgcgcatg atcaatgcgg tcattcccta cgtcaccaac
     1381 ctggattacg tctcctattc gtcatacgac ggcatgaatc tgagcgcttc ggccctccac
     1441 acgacgctcg actacatgca ggcgcacctg cccacgaaca aggccggcgt ggtgccgggg
     1501 gaacgaatgt ggatcggcga atatggctgg ggcggcaact cgaccgattc ccaggaaccg
     1561 ctcaaccgcg cctatatcca gcggctgctg ggttggaatt ccggcgggca gtgtctccag
     1621 ttcatcctgt tctgggagat atacgataat gaggtgggca agtatttctg cctgatagac
     1681 tcgaacaatg tccggaccgc gtcgtggtcc cttcatcagc gtttcatcag ccaggggcgg
     1741 ttgctcaccg cccaattcaa ggagaccaac ggccggctgc ccactgacac ggagttctct
     1801 tcgctgatga ttccgcggtt gagccaaccg ttgccggcgc cggtgaacct gacggtggcc
     1861 aatggcggcg tgaccgccgt caccgggacg tccgcagccg tggcgggcac ggtggcgcag
     1921 gggatttatg gcagcgacca ggccacgctt tgggtctttt gcggcagcca cgatggcggc
     1981 accgtgcggg ccaattggca gcaggcgatc cgggtggcgg tgaacaccaa cttcaacccc
     2041 gttaccctga gcacgacgct gaccaacctt gcgccgggca cgaactattt ctaccggttc
     2101 tatgccacca acgccaccgg cgaggcctgg gcgccggctt cggctgcgtt cgccaccgga
     2161 ttcgattcgc aggcgttcgg ctcgcggatg aagatcgttt tcagcggcta tacgcgaggc
     2221 gaagcgctga ccaacttccc ggtgctggtc acgctcggca cgagccagac aggtttctcc
     2281 taccgccaat tcgcctcgcc gacgggtggc gatttgcgct tcacggactt ctcgggcacg
     2341 gtgccggtct tgcacgagat agatgagtgg aacaccaacg gcgtctcgag tgtttgggtg
     2401 cggttgccgg tgctggcgag cgcgaacgat ttcatctggg catactgggg taacccttcc
     2461 gccacaaatc cgccgcccta taccacggac ggctcggtct ggacgccaaa ctacttcgtg
     2521 gtctatcacc tcaaggagag cggtttcccg tatgccgaca gcgcgcagca atatcccgcg
     2581 ctctccggca cggcccccgg ttccgcggtg ggaaagatcg ggcggggatg ttctttcaac
     2641 ggcgcgctgt cccaatacct taacgcgggc gtcatcaacc ttgggaacgc cttcacgctt
     2701 tccgtgtggg cgaacctggc cgccaatgcc tttgatattc agaccatctg ggccaaccag
     2761 ccagccggct acgggtcgcc tggctttgcc tggttcgcca atacctacaa gacgaacgac
     2821 cagaagctgg atttcgccag cggcgatggc gtcaacggca atgagtccaa gaccgccgcc
     2881 ggcgcagtca gctttggcca atggcacctg ctgacggcgt cggtgaacca gaccaagggc
     2941 agcgttgagt tctatgtgga tggcgccgat ctgggcggct ccagttcagt cgtcacgagc
     3001 ttcccgagcc aggccacggt caacctcggc cgctttacca atagcgcgtt ctatttcaat
     3061 ggcatcctgg acgaggcgcg gatcggggtg ggcgcttgct cgtcgaactg ggtctgggcg
     3121 agttggatga ccgtcgcttc gaacaccgct ttcgcggctc cctcttccgt aatgcgacaa
     3181 tcgccgacgc tcgggctcgc tgcgggcgcg ggcggactgg tcttgagctg gccggcgagc
     3241 ggcgtcgggt tcgcgctgta cacggccacc aacctgattc cctctgtcgt gtggactctc
     3301 gtcacgaacg cgccggcgct ggtcaacaac cagtggcaga tcccgctacc acgggacggc
     3361 agcgccgcct gcttctgtcg gctccaggct caatgaccgc ggatttctgg cagcggcggc
     3421 taaatccgct tctcgtcggc gtcgatgaaa tcgacgaagc actggatatc ctcgcgctgc
     3481 gccagtccgg cctgctcttt gcgcagcttc agccgcgcct gcatctccgc atcgtccttt
     3541 ctggggtagt ctaacatccg cgcccgatgg gccgccttta ccgaatccgg ctgcttcaca
     3601 ttcttgagca cccagtcgca gacttccccg tccgtgaggc tgtggcgcac cacttcgatg
     3661 aactgcttgg cctcgactcc ggcggtctcc agccacaggc cgtcaaagcc cttgcagagg
     3721 tttggctggt aatcgggatg caacttgccg gccaggtgaa ggcggacttt atcaatgaac
     3781 cgcggcaggt gcatccagcc gcccatcact tcgcgcggac tccggggata aatcatctcg
     3841 ctcatgcgcc aatcatcggc gctccaccca acgctgtcaa ccaccgctgc caacgcgcca
     3901 gatagatcca tcatccataa gctcgcagcc gcgcgaatta cggcgacttc cgccagcgct
     3961 gccagtcggt aagcaccaat tgttcgcgcc cccagccgtc tggcatttgg ttgagtggga
     4021 aatcatacgg caccctcttc ttcaccatgc tatccataat gaagaagccc cagcttagat
     4081 caccggcctg ggcccattga gtcaggaacc gcgcgtccat ggtctgcggc gtcaggtaga
     4141 gggcgttgat cggtttcatg tagtcgttaa tggcgaagaa atcttcgcgc gcggtggagt
     4201 cggtaacgtc gggtctgcac ttgagggtca accagacgca ttggcgctgg ccgtaccaag
     4261 ctacggccca cgggatgtcg ctcatgacca gttccttttc cttcagccag cccgccacgg
     4321 tctggatggc gggtggcatg tagggcgggt aaacgaccgg acgggtgctg gggggcagga
     4381 aaacaaaaag catgggcagg caagcaacca cgctgaaaag gccgacgacg aggtaacgca
     4441 attgagggaa caccatggga atctgctcga tcagcacaaa gaacaaactc acaccataaa
     4501 tcagcaccaa cggtgccacc agcaccagca gattctcgga gttaatctcg tgggactcct
     4561 cggagagctg ggtccgtccc agggcctggg ccaaaaccag caccacaatg caccccacca
     4621 ggaagtagcg caatcgcgtg agcgcgggac tgcgaaagcc gatcatcagc ccgaccagga
     4681 aaaacgccgc aacccaactc ccgccgagcc ggggcaactc gtccgtgatg acttggcgca
     4741 gattagtgtt cagcttcagc cagaaggcaa tcacgtagag gcggctgaag tccggctcca
     4801 gcgatcgctg caggcggttt tcggggtaga acatggtggt ttccacgacg gagtaggtgg
     4861 ccgtgccgaa ggggctgccg ctgacgttgt agttgcggac gatccacggc cccatgacga
     4921 gcgcaaacgc cgccaggacg atcaaggtta gcacggtccg ccgccgcccg ctaaacagaa
     4981 tcaggaacac aatgacaggg atgatcagcc acccgaacga gtagcgcgtc agcccaccca
     5041 gcccgaccat cgctccggtc aggccggcca gaaccaagac gccgtaaggt ccccactttg
     5101 gctcgcgtat ctgctcctcc agcagaacca ggcaccaggc caaccccatg aagatcagca
     5161 gtagcaacat ggttgacagc cccgacacgc tgaaccgcca aaacagttcc gtgccgagca
     5221 gcaggccggc cgagagccag gccactcccg gatcaaacag gcgccgggcc aataaaaaga
     5281 ccagcgcgat gaccgccaga aacagaagct gattaaacag ggcgatgagg aaatcgggtt
     5341 tgaagcgcga gaacgacttg ggggacgaga tcgtgtaatt gaagggcagg actttcatca
     5401 gcgcggccag cgccaccgga taaaccggcg ggttggacac gtcgggatgc atgccccgga
     5461 gttccgccag atccttggtc ttgccaatct gtggcatgct ctccttctcc atattgcgcg
     5521 ccttcaccag gtggatgctg aacgggcgga tgaacagagt ggtgtagccc ttgccctgcg
     5581 cgatattgcg gcctagttgc gccatgtcca ttgcctcctg cgtggccata tttctgaacg
     5641 cgcgccagtc gtagagcccg gtcagcagaa cgaccgccag cacggtcagg cccgcgcgca
     5701 gaaaacgcat ggtcccgccc acttcaaact tgtgcaacac ttcgtgtaag aacggcatag
     5761 ctaaatctcc tcaagatggt gcaggtgcag cctgcggtgg aaggtgcgcc ggctcatgcc
     5821 aagccgcttg gcggcgagcg tccggtgtcc gttggtggct tttagggcgc ggatgatcaa
     5881 ctctcgctcg gcttcctgtg ccgtcaaatc gctgcgcact acctgcccct tcgggtcctt
     5941 ctcggccggc gccctgccga cggcctcgcg cagcgaggtc ggcaaatcgc gcaccgtgac
     6001 tttgtcgccc cggcacagca ccacggcgtg ctcgatcgcc gtgcgcaact cgcgcacgtt
     6061 gcctggccat tggtagtgca tgagaagctc gaacgcatcg gcggtaaagc tctccaccgg
     6121 cttgctgttt tcctggctga actctcgcag gaactcccgc gccagcaacg gaatatcctc
     6181 caaccgctcg cgcaaggccg gcagccggat ttccaccacc cgcagccgga agaacaaatc
     6241 ctcacgaaac gcccccgtct tcacctgctc ctccaggtct ttgttcgtcg ccgcaatcag
     6301 ccgcacgtcg gcgctctgcg tcttgttcga gcccacccgt tcgaacgtcc gttcccccag
     6361 aaagcgcagc agcttgacct gggtcgaggc atcgatctcg ccgatttcgt ccaggaacaa
     6421 cgtgcctcct tgcgcctgct cgaagcggcc aatgcgccgc tcgtaagctc cggtgaatgc
     6481 gcccttctca tggccgaaca gctcgctctc cagcaacgtc ggcgccagcg ccgcgcaatg
     6541 caccgtcacc atcggctgct gcgcccgcgg gctgagctga tgaatcacct tggcgaccat
     6601 ctctttgccc gtgccggtct cgcccagcaa cagcaccgtc gcccgcgtcg gcgccacctg
     6661 ctgcaccacc tcgagcacct ccttcatggc ccgcgactcg ccgacgaggt tttgcgcgcc
     6721 gaatttcgcg tcgagctgct ggtgcagtga aacgttttcc acttccaggt tctgccggcg
     6781 caacgcgttg gcgatgcgca cctccagctc gtcgatttgc atccgcccct tggcgatgta
     6841 atcgtccgcc ccgcgtttca tcgcctcgac cgccagctcc tccgagccgt aggccgtcat
     6901 caggatgcag atgggcggtt tggacagcga cttggcccgc gtgatcagct tcatcccgtc
     6961 ctcggtcggc agccggaaat ccgtcaacag cacgtcgaaa tgatccttct ccagcaactc
     7021 caccgcggcc ttggcatcct cggccaggta cacgtcgtaa tgcggttcga gcgccgcgcg
     7081 aagaccttcg cgcgtcgttt tctcgtcatc aactatcagc aatgtcggag tcatgtcccg
     7141 gtcgggcgcc agtggcagat gccatcatgc aggatagagc agcgcctggg gattaaacaa
     7201 tcggcaaatt gcgccctgga gggcttaacc ccggcttgtc tttcgcgccc cgggggcgta
     7261 tgctcgcgga tgtgaacgga aagtggagca agtggttgcc gtatgttttc atgctggtcg
     7321 cggcgttgtc gcgctggccg aagctgttcc cgccgaattt cagcgcgttt tacgggctgg
     7381 ctttctgcgc cggcgccttc ttcccgcgca acgtcaaatg gtggctgccg cttgggacgc
     7441 tggtggtgac ggacatcgcc ctggacttct attaccagtc gttcagcgcg atgcaattgg
     7501 tcaactacgc ggcttatgcg ctcctcatct tgttcggcac acggttcagt catcgctcgc
     7561 gttttctgaa cctgctggcg ggcgggatta tgggggcgat cctgttttac ctgatcacga
     7621 ataccgcgtc atggctctcc aacccggagt acgcgaagga cctggccggc tggatcaagg
     7681 cgctgacgac aggcaccgcc ggttacccca ccacgcttga attcttccgc aacacgctga
     7741 tgagcggcgg cctgttcacg ggcctgtttg ccggcgccct caaactcacc gaggccgccg
     7801 aaccggttaa agagaaggag cccgaagagg agcccgccca agcccccgag ggcgaagagg
     7861 tgcccgatga aggcaaagca tgaggagtga tgcgtgatgc ttgacgcgtg atgcgtgatg
     7921 cgtgacggca acaagagccc tcatcctgtg ctgctagctt ccttcacgaa tcacgcatca
     7981 cggatcatgc tgaaaatcgg cgtcatctcg gacacgcatg gttacttcga cccccgcatc
     8041 ccgcatctct ttgccggcgt ggaccatatc ctgcacgcgg gcgacatcgg cctgccgacc
     8101 atcattctgg cgctggagca aatcgcccct gtgacagcgg ttgccggcaa tacggatgat
     8161 ccgggtttcc attaccggct aacggaagtg gttgacctgg ccagccgcaa gttcctcgtg
     8221 caccatatcg tgaaccccca gtcgctcggc gacccgatca aaacccgcat cgcgcgcgag
     8281 cggccagacg tgatcgtgtt cggccatacg cacaagccgt tctctgagac catcggcggg
     8341 acgcgattct tcaacccggg ctacgccggc aagtcacggt ttggcctgga gcggagcgtg
     8401 gccatcctgc gttgtagcga gaagggaatc aggacggaat acttgccatt gcagagctga
     8461 tcccaaggtc ccagctctgc ctgtttgcct ggacatttgc ggcagtcatt tcatcccaag
     8521 ccaggccagg acgaggttga gcagcagcgg acgcatgact atttcttgtc gtcggccaag
     8581 agcaaattcg ggttttcacc gggggcgatg aggtgtcgcg caatcatttg gtcaatgacc
     8641 gtgccgctca attccccgta gcaacgctgc aactcttcgc ggaagagctt ggcatcctgg
     8701 gctgcccagg ccgtgaactt gtgcgcgcgg ccacggtaat gcaccgggca cgagtagatt
     8761 tcccgaccgt cgtcgcctcg cagcaacgtc gcctgcgctt caaggtgaac ggccagcgaa
     8821 ggattgacgc tctcgttgcc cttcaacgcc gcactgagca cttcgattct cagcgccttg
     8881 tcccccgcgc gggggttcgt cgtcccatcg ccatgccaac ctaccagtat cggctcgccg
     8941 gtcgtaggct gtaccagtgc gacttgctct ccaccgtcgc ggggtttgtc cagcagcacc
     9001 accatcccgg aacagcgctg gctcagcgtc tgagcgatta cccgggccaa atcgtccgcg
     9061 ggccggctgc tccgtgcagc cgtcgttacc tgggtattgg cggcgctata tttcttgtcc
     9121 gacagtccgc gaatgcctcc ggcggtttgc aggtaaatgc tcaccggaat ggcggccgcg
     9181 atggcggtga gcgaaacgaa catgttggga tgagtgatca gcccgtccat catctcgttg
     9241 acgtcggact gcgcttcgga agcggcctca tctttagtca gcggcctttg aatgctcatg
     9301 aaatcgcccc gggaggggga gtaaacataa agggttcccg gactggcaac gacctgggac
     9361 acaagttgaa cctgcggcgc cagggccgga gcgggactat ggtcggcgag cctgacttgc
     9421 gggcgcgggg tgtgaaccga cggcttcgcg gcgccgacgc caaccaggat ggtggcttcc
     9481 cccgtgacct cgaacatgcg ctcgacaatt tgatcggcca gccggcgata accgaagtcc
     9541 gtgcatttca taaacggctc gccgttgttc agcgcccaat cgaggaacaa gtcttggccg
     9601 ctttgatact caaacgtttc atcgctgatt acttcgccag tgctggaacg caacaatcgc
     9661 acccgcgcct tgatccgcaa ggcaaacgag gaatcgcccg cgcctttgcg ctcgagccgg
     9721 agttcgtcgg cgcgcgtttc aagaatggtg cccacgggcg gagtggctgt gagttcgtga
     9781 ttctcgcgct gctgcgcggg aacgtcagac gccggcaatg ccacaaaacg acgccgaccg
     9841 gcggctttgg ccgcgtcgag aatccggtcg cgcaggtgat gttgtcccga catgttggtt
     9901 agggcttggg ccaggccggc ctcgcactcg gcgagtttgt cctgtggcag ccggctgcga
     9961 gaggcgagga caccgccgat taccgcggcc ggtgctgccg cgacaaatcc ggctgctgac
    10021 acgatgatat tggcctccgg attctccggc gtggtcatct ccagggcagc acgggcggca
    10081 tcgccggcac cgtctgccgc gcggttgatt tgtccccggg gcgggtcgta actgcacatg
    10141 gccggacgtt cgacggtgat gaccccgacc gggccggggt ccagcaggat gagttcaccg
    10201 gcaaaccggg cactggtaac atggacgctc cccagcttcg aggccataca actgatttgc
    10261 ccggtcatta cgatcaccaa tatccccaaa ccgatgggga gcaccccgga agaaacgatc
    10321 cgctgagtga acagcgagga agttggagaa ttagttttca tgccatccgt tctaactcaa
    10381 cttgccatgc gggtgttaat tgataaaact gtggcagcgt tcggtttagc caatagcgtg
    10441 aacattcatc acttggagtt gttctattac gtggcgcggc acggtggcat cagcgaggcg
    10501 atgcgccaca tgccgtacgg cattcagcag ccggctatca gcgatcaggt catccaattg
    10561 gaggaatcgc tcggggtgac gttgttccaa cgccggccgt tctctctcac gccggctggc
    10621 aaggagctgt acgaatccat catgccgttc ttcgacagcc tgacaccgat ggcggataga
    10681 ctgcggggcg gcgtctcgca gcatgtgcgc attgcggcgt cggagaccgt cttgcgggac
    10741 catctgccgg ctgccttgca ggaactgcgc aagcaattcc caaagctgaa ggtgacgttg
    10801 cgcgaagggt acgatcccga ggtcatctcc tggctggaac agcaggagat tgatgtcgcg
    10861 atcgggtttc tggggtccaa accgccgacg ggcatccacg cgctgccaat aaccaggttg
    10921 ccgctgatcc tgctcgtgcc gcggagcagc aaactcaagt ccgccgatga actgtggcag
    10981 cgcgaccgta tcgaggaatc gctgatcact gtgcccagta atgagccgat gtgccgcgcg
    11041 tttcaggagg gtctggggaa actcaaggtg gactggttta gcggcatcgg agtcagcacg
    11101 cttgagatgg tgcaaaccta tgtggctaac ggctacggca tcggcgtcac ggtcggcatc
    11161 ccccaggcga aataccatcc gcaggtgcgg gtgctgcggc tcgaaggttt tccgatgccc
    11221 gtgtttggtg tgctgtggca gggccgcccc acccccgtag tcagcggtct catcgagatc
    11281 gtgcgccagg cgaccgtcga gctgttgcgt ggcgaagatc cggctttgct cctgcccggc
    11341 agcgagctgg cgatggcggg gcagggcagg ggataatcgc cgccacgccc tattcctgcc
    11401 atgggagcct tagtgtggag cttcacataa gggcatcggg tccaggtggc gggtgagttg
    11461 caacttgccc aaagtatccc ctcgcttaaa atccaagcat gaccgtaagg cagttttacg
    11521 actggcagac agcgggcggc ggggacgtct cactgctcgt cgaaacgccg gaacgccgcg
    11581 ccagcaaacg ccagaaagac ctgctggaca ccatgcggtt ggtcgaagcg catcccaaac
    11641 tccgcaagct tctgcccgcc gacctggcga agaagatcaa aggatgattc accggcatct
    11701 taatcatcag gagttgagcc tggcggccat tgacgatgtg atcgcccgtg gcaagcgcaa
    11761 ggattgggag gagttgcgtc tggcggtcct ggctagccct gcattcctgg aaaagataca
    11821 acaggtgtgc cgcgcgcaca gtgccaacgt gaactgcgac aaacagcaca tctcgttgcg
    11881 tcgcgggttc acgttgattg aactcctggt cgtcatcgcg atcatcgcca ttctcgccgc
    11941 cttgttgttg cccgcgctgg ccagggccaa ggcgactgcg aagaccgcgc agtgtgtgag
    12001 caatctacat caaatgggtt tggccttgat catgtacgcc gacgagaacg atgggctggc
    12061 ggcgcgggcc aatggcccgc attggtggca ggtgctggcc ccgaatctcg gcggccaaac
    12121 cggcgcgaac tccaccaaag tcaggcttta cacctgtccg agctatcccg atccggaccc
    12181 gcattacccg ggccagaagc agttggtctg ctacgtggtg aacggatgga agttcgccag
    12241 cccgaccgac ccgacgggca cggagctgac cggcctgtca aagatcacaa tgattcagcg
    12301 tccagtggac acgatttacc tggccgaccg tgaggatggc accgactacg gcccgatcac
    12361 gacggccgac cccaatacat actcggatta ctacgacgtc tgggaaccca gccacctgcc
    12421 ttatctggcc aatggaaccg cgagtccccg caacgggctc tcgaacaatg ccaggcgggt
    12481 ggcgatcagc cgtcacacga aaagcgacgc cctgctgtac ttcgacaccc atgccgccac
    12541 gaagaagacg aggtt
//
LOCUS       DATCGS010000325         5932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_26875, whole genome shotgun sequence.
ACCESSION   DATCGS010000325 DATCGS010000000
VERSION     DATCGS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26875"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..2706
                     /locus_tag="VIL39_06635"
     CDS             154..2706
                     /locus_tag="VIL39_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5481139.1"
                     /translation="MSLNHSSAAPIPAIPPGVKLTTLDNGLTLIVREDHSAPVVSAQV
                     WSMAGSIHEGKWLGAGLSHVLEHMLFKGTTTRPGSRIDQEVQEAGGYMNAYTSFDRTV
                     FHIDVPNTGAKVALDILCDIMQNATLPPDEMAKEKQVILREMDMNVDDPGRRAGRRLF
                     ETAYTRSPYRFTVIGYPDIFNELSSEDILAYYREKYAPNNLFFVVAGDVKHDDVVAQI
                     RAAFAKAKARALPPVVLLAEPKQTAPREIIEEAAIELGYFYFAWHIPELSHADLPLLD
                     VLAVLLGSGRSSRLYQAVRERQGLVNSVDAWTYSPGNPGLFGLSATVDADKFAAARDA
                     MLAEVETMKTALVLESELSKAVKQFISATLSSRKTMQGQAQDLGGSWLAANDLNFSER
                     YLAAVKRVTPADLKRVAREYLTPENRTLYALLPTGATPKPAIEDETSRDQPVQKFTFA
                     KGLRLLVKEDHRLPFVEFRAVFQGGVLAETLANNGVTQLTGKMLLKGTARRSAEDIAR
                     EIESVGGSIDSYGGNNSYGVNAEVLSADFVMGLDLLADVLLEPTFPAPALERERQVQL
                     AGIRAQKDHLLQSASLAMRRTLFGERGYGLDASGNEGSVQSIQAADLKAFHQRLAVPN
                     NCVLAIYGDVKADEVRTAVEKAFVSWKPAAPPTLDFGLWTLDSIQRVTETRDKKQAVL
                     VIGFRGTTVHDADRYPLELLQEACSDLGSRLFMRIREKLGLAYYVGAQNVLGLAPGYF
                     AFYAGTTPEKAGLVETELLSEAELLRAEGLTPEELKRAKAKIIGQRKIARQDLGGFAM
                     ANALDELYGLGYAHTDAEDPRYEAVTLDEVKAVARKYLKPDALVIAVVRPEE"
     gene            3345..3986
                     /locus_tag="VIL39_06640"
     CDS             3345..3986
                     /locus_tag="VIL39_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5481140.1"
                     /translation="MIREKDSKLKLRLLSLAQKQRLIRFHFTTAAERNVEASRAFIAL
                     GDTARGAVPALARMYDEDLSANSLGAIADALAWIGPASKPAISVLLRAATNSDSKVRA
                     NALWALGEIRAEPKLCVPALVHALGDSDEWAQLSAAHALGMFGTEAQSAIPSLRELTN
                     SPRVFNSPVMTRLQVTLEAKNALRKIDSGAVSPSSKTFPESAIPAMDWFLSPQ"
     gene            complement(4130..5704)
                     /locus_tag="VIL39_06645"
     CDS             complement(4130..5704)
                     /locus_tag="VIL39_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481141.1"
                     /translation="MKTSGTPNESHLPGVSRRKFLAAGGAAAAVFTIVPRHVLGGAGF
                     VAPSEKITVAHIGCGTEGLRELPDMLRLPEVQIVAVCDPVKEGHDYVDWSKDGLRASI
                     AETLGKPDWRRGAPGIPGGRDVLKEVIESFYASQRRSEKFGGCAAYADFRELLEKEKD
                     VNAVKIMTPDHLHATIAIAAMKKGKHVMMHKPIANRLQEARLVIETARKTKVATHFLP
                     ASDGANIQRIKGWVDAGAIGALREIHNWSNRPVWPQYPTIPTDTPPVPAGFDWKLWLG
                     PSLDRPYHPHYTHAVFRGWYEFGGGALADMGHYSLWPVFNLFELDAPVMVESRPSHLC
                     TLTANVAVKIRNDYSFPAACTIRFKFAAKGARPPLDLFWYDGSMRPPTPEELEADNQE
                     LPAEGIMFVGDKGKILAGFLGQDPHIIPEKKMREYQASENPPGAARSGQGGQARRDAG
                     ALWVAACKGGQATYGDFLLAGPISDAFNLGAVSLRLGGKRLLFDAASGKITNVAEANK
                     CLGREYRPGWELAGLG"
BASE COUNT         1133 a         1908 c         1762 g         1129 t
ORIGIN      
        1 acgaacaggg acagccgcgc tcacttcgag aatggctacg actttgacga agcgttccgc
       61 aagccgttcc tgccgaggag caagcaaacc atccaagcgc ctgaggccaa gccggcgcag
      121 tcaccggagc ccaagtctta accgcgcgat tggatgagcc tcaatcattc cagcgcggcc
      181 cccatccccg ccatcccgcc gggcgtcaaa ctgaccacgc tcgacaacgg cctgaccctg
      241 atcgtgcgcg aggatcacag cgcgccggtg gtctcggccc aggtttggtc catggccggc
      301 agcattcacg agggcaaatg gctgggcgcg ggcctgtcgc acgtgctcga gcacatgctc
      361 ttcaaaggca ccaccacccg tcccggcagc cgcattgatc aggaggtcca ggaagcgggc
      421 ggctacatga acgcctacac gtcgttcgac cgcaccgttt ttcatatcga cgtgcccaac
      481 accggcgcga aggtcgccct cgatatcctc tgcgacatca tgcagaacgc gaccctgccg
      541 ccggacgaga tggccaaaga aaagcaggtc atcctccgcg aaatggacat gaacgtggac
      601 gacccgggcc gccgggcggg ccggcgcctg ttcgaaaccg cttataccag gagcccctat
      661 cgcttcaccg tcatcggcta cccggacatt ttcaacgagc tgagctcgga ggacatcctg
      721 gcgtattacc gcgagaaata tgctcccaac aacctcttct tcgtcgtcgc cggcgatgtg
      781 aagcatgatg acgtcgtcgc ccaaatccgc gcggcctttg ccaaagccaa agcccgggcg
      841 ctgccacccg tcgtgctgct cgccgaacca aagcagaccg cgccgcgcga gataatcgaa
      901 gaggcggcga tcgagctggg ttatttttac tttgcctggc acatccccga attgagccat
      961 gccgacctgc ccctgctcga tgtgctggcg gtcttgctcg gcagcggccg cagctcgcgg
     1021 ctctaccagg ccgtccgcga acgacagggc ctggtgaact ccgtcgatgc gtggacctac
     1081 agcccgggca atcccggcct cttcggcctg agcgccacgg tggatgcgga taagttcgcc
     1141 gccgcccgtg acgccatgtt ggccgaagtg gaaaccatga agacggcgct ggtcctggag
     1201 agcgaattga gcaaagcggt gaagcaattc atctccgcca ctctctcctc gcgcaagacc
     1261 atgcaaggcc aggcgcagga cctgggcgga agctggctgg cggccaacga cctgaacttt
     1321 tccgagcgtt acctggccgc cgtcaaacgc gtcacgcccg ccgacctcaa gcgcgtcgcc
     1381 cgtgagtatc tcacgccgga aaaccgcacc ctctatgcgc tcctgcccac cggcgcgaca
     1441 cccaagccgg ccatcgagga cgaaacctcg cgggaccagc cggtgcagaa gttcacattc
     1501 gccaagggcc tgcgcctgct ggtgaaagaa gaccaccggc ttccgttcgt cgagtttcgc
     1561 gcggtgttcc agggcggggt gctggccgaa accctggcca acaacggcgt cacccaactc
     1621 accggcaaga tgctgctcaa gggcaccgcg cggcgttccg ccgaggacat tgccagggaa
     1681 atcgagtccg tgggcggtag cattgacagc tacggcggca acaacagcta cggcgtcaac
     1741 gccgaagtcc tcagcgccga tttcgtcatg ggattggacc tgctggccga cgtgctcttg
     1801 gaaccgacct tccccgcccc ggcgctggag cgcgagcgcc aggttcagtt ggccggcatt
     1861 cgcgcgcaaa aggatcacct gctccaaagc gcctccctgg cgatgcgccg gacgttgttc
     1921 ggcgagcgcg ggtatggcct cgacgcgtcc ggcaacgagg gcagcgtcca gagcatccag
     1981 gccgccgatt tgaaagcctt ccaccagcga ctggccgttc ccaacaactg cgtcctggcc
     2041 atctatggcg atgtcaaagc cgacgaagtc agaaccgccg tggaaaaggc tttcgtctcc
     2101 tggaaacccg ccgccccccc gactttggac tttggacttt ggaccttgga ctcaatccaa
     2161 cgcgtcaccg agacgcgcga caagaagcag gcggtcctgg tcatcgggtt ccgcggcacg
     2221 actgtccacg atgccgaccg gtatccgctg gagctgctgc aggaggcttg cagcgacctg
     2281 ggctcgcgcc tcttcatgcg catccgcgag aagcttgggc tggcctatta cgtcggcgcg
     2341 cagaacgtcc tcggcctggc gcccggatac tttgcctttt acgccggcac gacgccggag
     2401 aaggccggcc tggtggaaac cgagttgctg agcgaggccg aattgctgcg agccgagggg
     2461 ctcacacccg aagaactgaa acgcgccaag gccaagatca ttggtcaaag gaagattgca
     2521 cgccaggacc tgggcggctt cgccatggcc aatgcgctcg acgaactcta cggcctcggc
     2581 tacgcccaca ccgacgccga agacccgcgc tacgaggccg tcacgctgga cgaggtcaaa
     2641 gccgtcgccc ggaagtatct aaagccggat gccctggtaa tagcggtggt gagacccgaa
     2701 gagtagaggc caggctcgat actccacggc gcagcggagc cggaaccgag agcggaacgc
     2761 ctttttaacc gcagagacgc gaagagcgca gagaagcgaa acagagcaga atctccgcgc
     2821 tcttcgcgtc tcggcggtta aggatgggtg ccgaacattt gcacaggcag ggaaaagacc
     2881 accgaggagg acctccgata atcagtgatg gcggcccggt gcccggcggg catgggcgtc
     2941 ggcatccgcg cgcggcgagc tgatttcgtc tgggcggtct cattacttaa tcccttgtca
     3001 tatcctgccg ggtcgattcg gcgattgacg gggagggcct ggaactacaa gcattgccca
     3061 tgagcgacga acataggaac gcgtgcttgc ctctgagtga gccgcattcc aatccgtggt
     3121 ggctgagggc ctttgctgcg tcgatcattg gcctccttgt ggggtcagtt tggttgttgg
     3181 cgcgccaagg tcctcgtgtt ccagtttact gcggcaaatc gttgcccatt tggctgcgaa
     3241 actacggctg gtcttcgtca gctaggcttc attctcgaga gtggaacgaa gcggatgatg
     3301 ctgtgcgcca catgggaacg aactgcattc ccgtcctgct gcgcatgatt cgagagaagg
     3361 actccaagtt gaagcttcgc ctgctttctt tggcacagaa gcaacggcta ataagattcc
     3421 atttcactac ggccgcagag aggaatgttg aggcgtcgag ggcgtttatc gctttaggtg
     3481 atacggctag gggcgcagtg cccgcgttag caaggatgta tgacgaagat ctgtctgcca
     3541 actctctggg cgcaattgcg gatgccttgg cttggatcgg gccggcttcg aagcccgcga
     3601 tctcagtgct tttgcgagca gcaaccaatt cagactccaa ggtgcgggcg aacgctttat
     3661 gggcactggg tgaaatccgt gccgaaccta agttgtgtgt tcccgcgctc gtccacgctc
     3721 taggtgactc cgacgaatgg gcgcagctca gtgccgcgca tgctctcggt atgttcggta
     3781 ctgaggcgca gtctgcgatc ccctcgctga gggaactgac aaattccccc agggttttca
     3841 attcccccgt aatgacgagg ctgcaagtga cgttggaggc taagaacgcc ctgaggaaga
     3901 tcgattccgg cgccgtttcg ccatcaagca agacctttcc tgagtctgct attcccgcaa
     3961 tggactggtt tctttcaccg caataactgg tagtagcgtt ccgcctcgtc tgggctggat
     4021 ggcggagtga gaggctaacg ctgaccaggg actgccaacg cctgtttcct tcgggtttcg
     4081 gatttcgggc ttctttcggc cttcggattt cgggtttcgg atttccgcct caccccaacc
     4141 ccgccagctc ccatcccggc cgatattcgc gaccgaggca cttattggct tccgccacgt
     4201 tggtaatctt gccgctggcg gcgtcgaaga gcagccgctt gcctcccaac cgcaaggaaa
     4261 ccgcccccag gttgaaggcg tccgagatcg gcccggccag caggaaatct ccgtacgtgg
     4321 cctggccacc cttgcacgct gccacccaca gtgctccggc gtcgcgccga gcctgcccac
     4381 cctgccctga ccgcgccgct cccggcgggt tctccgaagc ctgatattca cgcatcttct
     4441 tctcgggaat gatgtgcgga tcctggccga ggaaaccggc aaggatcttc cctttgtccc
     4501 ccacaaacat tatcccttcg gccgggagtt cctgattgtc cgcttccaac tcctcgggcg
     4561 tcgggggccg catcgaaccg tcataccaga acaggtccag cggcggccgg gcgcccttgg
     4621 cggcgaactt gaaacgaatc gtgcaggcag ccgggaagga atagtcgttc ctgatcttca
     4681 cagccacgtt ggccgtgagc gtgcagagat ggctgggccg tgattcgacc atcaccggag
     4741 cgtccagctc gaagagattg aagaccggcc agagactgta atggcccatg tcggccagcg
     4801 ccccgccgcc gaactcatac cagccgcgga acacggcatg ggtgtagtgc ggatgatagg
     4861 gccgatcgag cgacggcccc agccacagct tccagtcaaa gcccgccggg actggcggtg
     4921 tgtccgtcgg gatcgtcggg tattgcggcc ataccggccg gttcgaccag ttgtgaatct
     4981 cccgcagcgc gccaatcgcg ccggcgtcaa cccagccctt gatccgctga atgttggccc
     5041 cgtcactcgc cggcagaaaa tgcgtcgcaa ccttcgtctt gcgcgcggtc tcgatgacca
     5101 gccgcgcctc ctgcaagcgg ttggcgatcg gtttgtgcat catgacatgc ttgcccttct
     5161 tcatcgccgc gatggcaatc gtcgcgtgca ggtgatcggg cgtcatgatc ttgaccgcat
     5221 tcacgtcctt ttccttctcc aacagctcgc gaaaatcagc ataggcggcg cagccgccga
     5281 acttttccga acgcctctgg ctggcataga aggactcgat gacctccttg agaacgtcgc
     5341 gtccgccggg aatgccgggc gcgccccgtc tccagtccgg tttgcccagc gtctcggcga
     5401 tggatgcgcg caagccatct ttggaccagt ccacgtagtc gtggccttcc ttgacgggat
     5461 cgcacacggc gacaatctgc acctcgggca acctgagcat gtccggcaac tcgcgcaacc
     5521 cctcagtgcc gcaaccgatg tgggccacgg taatcttctc gctcggggcc acaaagccgg
     5581 ccccgcccag cacgtgccga ggcacaatcg taaacaccgc cgccgccgcc ccgccggccg
     5641 cgaggaactt ccgccgggaa acgcctggta aatgggattc gttcggtgtg ccgctagttt
     5701 tcatccctga cctcataacc aagatcactg gtccggcgca agcgagttct tctgttagca
     5761 atcgaaagac cccaggcatg gcagcgtggg gcaagccggg aggttgggag ttactcccgt
     5821 gtattcctct attgttcctc tattgttcct ctattgttcc tctattggtg ctctattgtt
     5881 cctctattgg tgctctattg ttccccgtgt ttccaccctt ggtttcccac gt
//
LOCUS       DATCGS010000326        12364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_78073, whole genome shotgun sequence.
ACCESSION   DATCGS010000326 DATCGS010000000
VERSION     DATCGS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78073"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..863
                     /locus_tag="VIL39_06650"
                     /pseudo
     CDS             648..863
                     /locus_tag="VIL39_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            879..1505
                     /locus_tag="VIL39_06655"
     CDS             879..1505
                     /locus_tag="VIL39_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481142.1"
                     /translation="MDIKSGGGIANWTDPTAWHIMLLPYAGFKVNVAPGGTVLNITPK
                     VFACPSEKVTDSFPRANGIQFQASYRVNLYLFRASGGSAKSAGPLRASLVPTTTQTLM
                     LTEKTYDSWDFQTSATDLNSMLVNWNSTSTSQKDYLTSGFGRHMGSSVATAADGHSVS
                     LKLASYQPGAPNPTTFIDLGDTRYDTGLWPQPTKPNLYLREKNTNDGF"
     gene            complement(1738..2823)
                     /locus_tag="VIL39_06660"
     CDS             complement(1738..2823)
                     /locus_tag="VIL39_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY5481143.1"
                     /translation="MHLAHLRLRDFRNYARLDVDFAPGFHLLLGDNAQGKTNILEAIY
                     LIATLRSFRGVGGAQMIRHGQKGYFVGGKVVGQGEHEVKMYWSSPERSLSLDARPVRK
                     LTDYLGVLRTVVFCTEDLQLVKGVARARRRFLDLLLSQTYPAYLPLLQRYTRALRSRN
                     ALLKHPRPDPAALDGFSRELVKSGEEIIRLRRELVPKFSPLARLAYRCISSEAEELRL
                     EYLPSVKQDFSVELAQSRQRESAYRSTLIGPHRDDFQLLLDDHSAAQFGSEGQKRTLA
                     ITLKLAQAEYLTGLHGSPPVLLIDDIMGELDVKRRGGFLPLLQRAQQSSGQVFMTATE
                     ENWPEEPGRELQHWRVKDGTLLKERGC"
     gene            3054..5006
                     /locus_tag="VIL39_06665"
     CDS             3054..5006
                     /locus_tag="VIL39_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481144.1"
                     /translation="MKKDSDSAVSKILVIDDEDSFRQMLIKSLSREGFEVEAASDGKA
                     GVDLAAETQPDLIVCDLTMPGMDGYEVLAALRRDPKLADIPLIFLTGQSQPSQVRQGM
                     NFGADDYLTKPVNLTDLLGAIRVRLGRRQAQQQRQEKQIERAMQLFGGIVHDLRDPLF
                     VVLGYTGMLKGGPGQAPAGGKNPRQILDRMQQAVLRMQAIISETLFLTRSRMQRLPFD
                     PGPFDLRDFCEQMVADHEENQRVRFQCAAGRFPVVADALRLRQALDNLLSNALKYSDR
                     PVVVSLSAGSQDYLIEVRDQGIGIPANDQAGIFEPFFRASNTGSKPGHGLGLSIVKSC
                     VEQHGGTIRFRSEGGHGTTFFLELPKAPLAGPAAQTGVLPPDGRTNVVANHPPETRPT
                     AAATSAGALPEKAGLLRGIIVDDDPLVRGLMRDLLETGNDILIVGEAGTVAQARSLAK
                     QHGPDVVFLDVGLPDGSGFDLLPDLKPDASVVFVTSAEEYAVHAFDCEAADYLLKPVS
                     PERLQQALARVRQRLAAKPGAAVASSKPGDSFLVKTLTEKRLVKVTEIERIVAYGEYS
                     WVYWQKNKKGALLRKSLKQWLSELPGEQFIRVHRRAIVNLACMERVEQLPNSRMQIHL
                     RDTAEPILVSLRLAPVLNRKLKAMRG"
     gene            5204..8452
                     /locus_tag="VIL39_06670"
     CDS             5204..8452
                     /locus_tag="VIL39_06670"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY5481145.1"
                     /translation="MKPNSNPRLTTPTRVVILIALYFLGGLLGKEASFLSGSVALVWP
                     PAGIALAAILLFGYRFWPGVALGAVLFSFMNGMPLGFFTFGTAIGNTMGAIVCTFLLR
                     RIINFDNALERTRDVAGYIGLACFLGTTVNAAFNVVGLVYGGTVAWPDLFPTILEWWV
                     PNALAGLVVAPFLLTWATPSALRWQPRLVVEAAVCGTGLVAGTLISFHSWFVYGIQNY
                     PLAYLPFPFLVWGALRFGQRGATTGTLLVSALAIHSLLSGRGPFLATGERDSLMLIGS
                     YIGVLAVTNMLLAAAAAERRQAERAMADSEKRLRAVVEDQTDLICRFKPDGILSFVNR
                     AYCQFHGKTREELLGTSFLQNLPLEDIAIPLSWFEALPPEQPVVSFDHRVVGRDGLAV
                     WQQFSVRRLFREGGQTLEFQAVIQDITHRKQTEQALRASEGKYRSLVANIPDIVWTAD
                     ANRKLLYVSDSVEKILGYTAGELIQGGGTLWLDRIHPSDFPRVQLAYQMLFAKEEAFD
                     VEYRIRRKDGQWIWLHNRTPSTYVRDGALGADGLLSDITQRKRVEEALQQAKDAAEAA
                     NRAKSQFLASMSHELRTPLNAIIGFSEILGDKTFGDLNDRQLKYSNNILNSGRHLLQL
                     INDILDLSKVEAGKLELTRSSFSVARALENAQVIIKTLACKKGIALDFDVAAAPSELF
                     ADEAKFKQIMYNLLSNAIKFTPDGGRVAVTATRGNHPNGQGAPWSRSPLSGECLEVTV
                     ADTGIGIHPRDHERIFAEFEQVDSSYGRLQQGTGLGLALTKRLIELHGGRIQVESEGI
                     EGKGSRFTFLLPLQKPEPKPAPAHDPAAPREDALLRPLVLIVAEDDQSQRLASSYLTG
                     VGYGVAVVARIQDLAATLKNNRPYAVVFDHQITGPRNEDELRDLRTRIPAGIPTVVFT
                     IDAEGKFGFIRFAAVRRPEALTRPRLIDALGRTSNSSGKEVKTVLIIEDEPALLELLT
                     KTLLFKGFQVLPAVNGRCGIEFAVGSHPDVIILDLTLPDRSGIQVVEDLRARPETKSI
                     PILIHTGTVLSEPERQRLAAQVQSITSKAEPQSLLTQLEHLDEAPAEAVLQE"
     gene            8457..8873
                     /locus_tag="VIL39_06675"
     CDS             8457..8873
                     /locus_tag="VIL39_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481146.1"
                     /translation="MKKRILVVEDNLLNLELVTDLLEVGGFIVCPARTGEEGLHAARE
                     QPPDLILMDLSLPGLDGLAATRSLRADPATRHLTIIALTAHAMNGDEALALRAGCDGY
                     LTKPIDTRTFAAKVAAFIAAADSRLTQPQALCQYAS"
     gene            8863..9942
                     /locus_tag="VIL39_06680"
     CDS             8863..9942
                     /locus_tag="VIL39_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5481147.1"
                     /translation="MQAETLIRNKSSAARAVGSRGLVLVVDDEEANRMLLRDPLEVHG
                     YEIIEAENGEQALQKVEQQPPDVILLDVMMPRMDGFEVCRRLKKEARTAHIPILIVTA
                     LSERMERMMGIAAGASDFLSKPVDLQELTLRVNHAVHAKRLVDQLQAEQVKVERLLLN
                     SLPGPIAERMKQGEVNIAEHHPDVSVLLADLVGFTTLAAHIGPEQVVYLLNEIFSGFD
                     LLALKHGLEKIKTIGDAYMAAGGVPLPRPDHAEAIAELALDMMAEIEKFNRAYNTSIQ
                     LRIGISTGSVVAGVIGRWKFTYDLWGDTVNLACRLESLGQAGTILVSDLTYQRLRDKY
                     RFDDSRSLEIKGRAAELVHTLRGRL"
     gene            10282..12030
                     /locus_tag="VIL39_06685"
     CDS             10282..12030
                     /locus_tag="VIL39_06685"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY5481148.1"
                     /translation="MNPRTAFNVPCSRGEQILRDPSQNKDAAFTHAERSKLGIEGLLP
                     SAVLTIQQQVALELEHIFQKPDPLEQYIGLIALLDRNEVLFYRLMVENLDRLCPIIYT
                     PTVGLACQQYSHIYRRPRGLFLCPDDRGQIAQRLRNFSQPDVRLIVVTDNERILGLGD
                     QGAGGMPIPIGKLVLYSAGAGIHPSLTLPISLDVGTDNSALIADPYYLGYRGRRLRGP
                     DYDSFVEEFVRAVKTVFPRALLQWEDFKKANAFRLLERYAGRLPSFNDDIQGTSAVVL
                     AGVLTGLRLIGKPVREQRFLLVGSGAAGVGIGRLLHTALLADGLTETQARQHEIFMDS
                     SGLVWEGRANLEEHKREVALRKDDLAAAGLSCPPPDSLEKLIRAVRPTVIIGTTGQPG
                     DFTPEAIRAMADNCERPIILPLSNPTSKAECTPSEALQHSDGRALVATGSPFDPVVYK
                     GRKYVIGQCNNAFVFPGVGLGVLISEASRVTDSMFLAAARALAEFTSTHAASDNCLYP
                     SLCELRNLSSLIAFRVARTARDEGFGNNLDDNALQAAIDDFCWFPEYPETDHSAHHPA
                     PAAEAPAHTTPPQNLL"
     gene            complement(12086..12304)
                     /locus_tag="VIL39_06690"
     CDS             complement(12086..12304)
                     /locus_tag="VIL39_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481149.1"
                     /translation="MGPRKPFLLRIDPVLWAELEVWAQAELRSVNGQIEYLLKQALQK
                     RKGATPRPAPPPANSAGAPDPGRSGNAA"
BASE COUNT         2392 a         4005 c         3682 g         2285 t
ORIGIN      
        1 aaccttctga ttccattata gatgcgtaac atgctcagga ataatatgtt atcaagtttt
       61 gtagggtcgt ggctgtaaag gcaggttttt gcggtttata ggactgacag agcagacggg
      121 ccagttgacg gcgggacaag ccggtgcctt cctgctccaa gcgctggagc aagtgaggcg
      181 agaagtgttg cccggccacg atcacgccgc gagcttggcg cacggcggcg gtggtgtcca
      241 gcgaaaaact tgtggcgcaa gatcagcttt cggccagggg ttggcgggct tgcttcatag
      301 cagattcgtt ctgggacggc tgtgttgctt acctatgagc cggaagaagc gatctgcaac
      361 atcgaccgac cttccaactc cataggctta atagccgcac gaccgggaat tgggacaagg
      421 aaatgggcct gctcgagctt cggccttcgg gcttcaggct tcgtaatggt tcgggtcgga
      481 gcgtggggca gacttgctta acgcgggctg gagcactccg ggaagcgccg caaccgcaag
      541 cctgtatcga gcgcattgtt ggcgcttccc gcgcctgcct ccacggcacc accacccaga
      601 tgatgggcca atacaaacgt gaaccccgac gtatgaacca acctgaaatg cccactgctg
      661 ctgaaccgcc cgcgcccgcg tcccgcccaa acgcgttcac cttgattgaa ctgctggtgg
      721 tcatcgccat catcgccatc ctggcggcgt tgctgctgcc ggcgttggcg cgggccaagg
      781 ggaaagccca ggcgatccaa tgcctgaaca acgcgcgcca gctcggcctc gctacgcatc
      841 tttacacgac agattacaat gactgctttc cctgaggcgt ggacattaag agcggaggcg
      901 ggatagccaa ttggaccgac ccgaccgcct ggcacatcat gctcctgccg tacgcaggct
      961 ttaaggtgaa tgtcgcgccc gggggcaccg tgctgaacat caccccgaag gtttttgcct
     1021 gtccctccga gaaggtcacc gactcgttcc cgcgggccaa cggcatccag ttccaggcta
     1081 gctatcgggt taatctatac ctgttccgtg cctcgggtgg cagcgcgaaa agcgcaggtc
     1141 ccttgcgcgc gagcctggtt cccacgacca cgcagacgct gatgctcacc gagaagacct
     1201 atgatagttg ggactttcag accagtgcca ctgacctgaa ctctatgctc gtcaactgga
     1261 actcgacttc cacctcgcag aaggactacc ttacttccgg cttcggccgc cacatgggca
     1321 gctctgtggc cacggccgcg gacggacatt cggtttccct gaaactggcg tcctatcaac
     1381 cgggggcccc taacccgact accttcattg acctcggcga cacgcgctac gacaccggcc
     1441 tctggcccca gcccaccaaa cccaaccttt acctgcgcga gaagaacaca aacgacgggt
     1501 tctgaccggc cgtccgcccg gtgcgcacaa ggtcgcgctg tcgctagcag cctgtcggac
     1561 ttaggcgcag aaatctccat aagtcattgc agttacaata ttattagcat tcctgcttaa
     1621 gtcattatta acattgctac atattgagga tgcgtgagat acgaagattg ggcatggcta
     1681 agtccgacac gctgctaggg aataagctgt tcatccactc ctccgaacgg gtgcacatta
     1741 gcagcctctc tccttaagca gtgttccgtc tttgacccgc cagtgttgca gctcgcgtcc
     1801 cggttcctcc ggccagtttt cctccgtggc cgtcatgaac acctggccgc tcgactgttg
     1861 cgcccgctgc agcaacggca gaaagccccc gcgccgcttg acgtccaact cgcccatgat
     1921 gtcgtcaatg agcaggaccg gcggcgagcc gtgcaggccg gtcagatact cggcctgcgc
     1981 cagcttgagg gtgatggcga gcgtgcgctt ctgcccctcg ctcccgaact gcgccgccga
     2041 atgatcgtcc agcaggagct gaaaatcatc ccggtgcggc ccgatgaggg tggagcgata
     2101 ggcgctctcg cgctgccggg attgtgccag ctccacgctg aagtcctgct ttacgctcgg
     2161 cagatactcc aggcgcagct cctcggcctc gctcgaaatg caccgatacg ccagccgggc
     2221 cagcggcgag aacttcggga ccagttcgcg gcgcagccgg atgatctcct cgccgctctt
     2281 gaccagctcc cgggaaaagc cgtccagcgc tgccggatcg ggcctcgggt gtttgagcag
     2341 cgcattgcgc gagcgcaacg cccgggtgta gcgctggagc agcggcaggt aggccggata
     2401 cgtttgcgac agcagcagat cgagaaagcg ccgccgggcc cgcgccaccc ctttgaccaa
     2461 ttgcaaatcc tccgtgcaga aaaccaccgt gcgcagcacg ccgaggtaat ccgtcagctt
     2521 ccgcaccggc cgggcatcca ggctcaggct ccgttccggg ctggaccagt acatcttaac
     2581 ctcgtgttcc ccctgcccaa ccaccttgcc gccgacgaag tagcctttct gcccatgccg
     2641 gatcatctgc gcgccgccca ccccgcgaaa cgagcgcagc gtcgcaatga gataaatggc
     2701 ttcgagaatg ttggtcttgc cctgggcatt gtcgcccaac agcaggtgaa aaccgggcgc
     2761 aaaatccaca tccagccgcg cgtaattgcg gaagtctcgg aggcgcaagt gggccagatg
     2821 catgccctca gaatggatat cgacggcccg aggggcgaga tcttttcaca ccaggcatgg
     2881 tggtgcccat ggccagtgac aaagccgccg atatacctcg ccccgcaagt atggactgcg
     2941 gccgttgggg aagggttaag aaccggttgg cattccgtgc gagctgtgaa aaacatccag
     3001 gctggcagca aatgcttggc aggaagcccg gttgggggca tgttgagggt gccgtgaaaa
     3061 aggattccga cagcgcggtg agcaaaatac tggtcattga tgacgaggat agctttcgtc
     3121 agatgctcat taaatccctg tcccgggagg ggtttgaggt ggaggcggcc tcggatggga
     3181 aggcaggagt ggacctggcg gccgagacgc aaccggacct gatcgtgtgc gacctgacca
     3241 tgcccggcat ggacggctac gaggtgttgg cggcgctgcg gcgcgacccg aagctcgccg
     3301 atatcccgct catctttctc acgggccaat cccagccaag ccaggtgcgc cagggcatga
     3361 acttcggggc ggatgactat ctgacgaagc cggtaaacct gacggacctg ctcggcgcca
     3421 tccgagtgag gctaggccgc cgccaggccc agcagcagag gcaggaaaag caaatagagc
     3481 gggccatgca gcttttcggc gggatcgtgc atgacctgcg tgacccgctc ttcgtggttc
     3541 tgggttacac cggcatgctg aagggcggcc ccggccaggc gccggcgggc gggaagaatc
     3601 cacggcaaat ccttgaccga atgcagcagg cggttctgcg catgcaggcg atcatctcgg
     3661 agacgctgtt cctaactcgt tcacggatgc agcggctgcc gttcgatccc gggccattcg
     3721 acctgcgtga tttttgcgag cagatggtgg cggaccacga ggagaaccag cgggtgcggt
     3781 ttcaatgcgc ggcggggcgg tttcccgtgg tcgccgacgc gctccgcctg cgccaggcgt
     3841 tggacaatct cttatccaat gcgctgaagt attcggatag gccggtcgtg gtcagcttga
     3901 gcgccggttc ccaggactac ctgatcgagg tcagagacca gggcatcggg ataccggcga
     3961 acgatcaggc cggcattttt gaacccttct tcagagcctc gaacaccggc agcaagcctg
     4021 ggcacggcct ggggctttcg attgtcaaaa gctgcgtcga acagcacggt ggcacgatcc
     4081 gcttccgcag cgagggaggc cacgggacaa cgttcttcct ggagctgccc aaggcacccc
     4141 tggcagggcc ggccgcgcag accggggtat tgcccccgga tggcagaacc aatgtcgtgg
     4201 ccaatcaccc gccggaaacc cggccaacag cagcagcgac gagtgccggg gctctgccgg
     4261 aaaaggcggg gctgttaagg gggatcattg tggacgacga cccgctggtg cgaggcctga
     4321 tgcgcgacct cctggaaacc ggcaacgaca tcctcattgt gggagaagcg gggacggtcg
     4381 cacaggcgcg ttcgctggcg aagcagcatg ggccggatgt cgtctttctg gatgtgggcc
     4441 tgccggatgg gtccgggttc gatttgctgc cggacctgaa gccggacgcg tcggtggtgt
     4501 tcgtgaccag cgcggaagag tatgccgtcc atgcctttga ttgcgaggcg gcggactatt
     4561 tactgaagcc cgtcagcccg gagcggctgc aacaggctct ggcgcgagtg cggcagcggt
     4621 tggccgcgaa acccggggcg gcagtagcaa gttccaagcc cggcgattcg ttcctggtca
     4681 agaccctgac ggagaaacgc ctggtgaagg tgactgagat cgagcgaatt gtcgcctacg
     4741 gcgaatactc gtgggtttac tggcagaaga acaagaaggg ggcattgctc cgaaagtcgc
     4801 tcaaacaatg gctgtcggaa ctgccgggcg agcagtttat ccgggttcac cgccgcgcga
     4861 tcgtgaacct cgcctgcatg gaacgcgtcg aacaactccc caacagccgg atgcaaatcc
     4921 atctgcgcga cacggcggag ccaatcctgg tgagtctgcg cctggcgccc gttctaaacc
     4981 gcaagctcaa ggccatgcgc ggttgagcgc ttgcgggcgc aagcacccat tccgaaccgc
     5041 ctgatccggc acctgctccc gcccagaagt gagacagccc cgccaacttc cctggctgct
     5101 ccggactaaa ggtgacacct gtgatgcgct tgctgcattt attgtggcgt tcactgcacc
     5161 taccagtctc ggcacgcttc ctgcgattac atctctgctt gaaatgaagc cgaactccaa
     5221 cccgagattg acgacgccga cgcgcgtggt gatcctgatc gcgctctact tcctcggggg
     5281 gttgctcggc aaagaggcgt ccttcctgtc cggcagcgtt gcgctggtat ggccgccggc
     5341 ggggattgcg ctggcagcga ttctgttgtt cggttaccgg ttctggccgg gcgtggccct
     5401 gggggcggtc ctgttttcct tcatgaacgg aatgccgttg ggcttcttta cgtttggcac
     5461 cgccataggc aacacgatgg gggccatcgt ttgcaccttc ctgcttagga gaatcatcaa
     5521 cttcgacaat gccctggagc gcacacggga cgtggccggc tatattggac tggcctgttt
     5581 tctggggact accgtcaacg cggcgtttaa cgtcgtgggc ctggtctatg gcggaacggt
     5641 ggcgtggccg gatttgttcc ccacgattct ggagtggtgg gtgcccaatg cgctggcggg
     5701 cctggtggta gcccccttcc tccttacctg ggcgacgccg tcagccctcc gttggcagcc
     5761 gcggttggtg gtggaagcgg ccgtttgcgg gacgggtctg gtggctggca ccctgatttc
     5821 cttccactcc tggtttgtct atgggattca gaactacccg cttgcttatc tgcccttccc
     5881 cttcctggtt tggggcgctc tgcggtttgg gcagcgggga gcaaccaccg gcacgctgct
     5941 cgtatcggcg ctggcgattc actcgctctt gagcgggcgc ggaccgttcc tggccaccgg
     6001 cgagcgggac agcctgatgc tgatcgggag ctacatcggc gttctggcgg taacgaacat
     6061 gctgttggcg gcagcggcgg ccgagcgccg gcaggctgaa cgggccatgg ccgatagcga
     6121 gaagcgtttg cgcgcggtcg tcgaagacca gaccgatttg atctgccggt tcaagcccga
     6181 cgggatcctc agcttcgtca accgcgccta ctgtcaattc cacggcaaga ccagagaaga
     6241 gctgctcgga acaagtttcc tgcaaaacct gccgctggaa gatatcgcga tccccttgag
     6301 ctggttcgag gccttgcccc cggagcaacc ggtggtttcc ttcgaccacc gggtcgtcgg
     6361 gcgcgacggt ctggcggtgt ggcaacagtt tagcgtccgg cgcctgtttc gggaaggggg
     6421 ccagacgctg gagtttcagg ccgtcatcca ggacatcact catcgcaaac agaccgagca
     6481 ggccctgcgc gccagcgagg ggaagtaccg ttcgcttgtc gccaacatcc cggacatcgt
     6541 ctggacggcc gacgcgaacc gcaagctcct ttacgtgagt gatagcgtgg aaaagatact
     6601 cggctacacg gctggcgaac ttatccaggg cgggggaacc ctgtggctgg atcgcatcca
     6661 tcccagcgac ttccccagag ttcagctcgc ctaccaaatg ctgttcgcca aagaggaagc
     6721 gttcgacgtg gaatatcgca tccgtcgcaa ggacggccag tggatttggc tgcacaaccg
     6781 tacgccctcg acctatgtgc gggacggcgc gcttggcgcc gacggcctgc tgtcagacat
     6841 cacgcagcgc aagcgggtgg aagaggcgct gcagcaggcg aaggacgccg cggaggccgc
     6901 caaccgcgcc aagagccagt tcctcgccag catgagccac gagttacgca ccccgctcaa
     6961 cgccattatc ggcttttccg agattcttgg cgacaagacc tttggcgact tgaacgaccg
     7021 gcagctcaag tactccaaca acattctcaa cagcggccgc cacctgctgc aattgatcaa
     7081 cgacattctc gacctctcca aggtggaagc cggaaagctg gagctgaccc gcagctcgtt
     7141 cagcgtggcc cgggcgctgg agaacgcgca ggtgatcatc aagacgctcg cctgcaagaa
     7201 gggcatcgcc ctcgacttcg acgtggcggc agctccctcc gagctgttcg ccgacgaggc
     7261 gaagttcaag caaatcatgt ataacctcct cagcaacgcc attaagttca ccccggacgg
     7321 agggcgagtt gcggtgaccg cgacgcgcgg gaaccacccg aacgggcaag gcgcgccgtg
     7381 gtcgaggtcc ccgctctcag gcgagtgtct ggaagtgacc gtggcggata ccggcatcgg
     7441 catccacccg cgagaccatg aacgcatttt cgccgagttc gagcaggtgg actcctccta
     7501 tggccggctc cagcaaggca ccggattagg actggctttg acgaagcggc tcatcgagtt
     7561 gcatggggga cgaatccaag tggaaagcga aggcatcgaa ggcaaaggca gccggttcac
     7621 tttcctgctc ccactgcaga aacccgaacc gaaacccgcg ccggcgcatg acccggcggc
     7681 accgcgcgaa gatgctctcc tccggcccct ggtcctgatc gtggccgagg atgaccaaag
     7741 ccaacgtttg gccagcagct acctaaccgg tgtgggctat ggcgttgcgg tcgtcgccag
     7801 aattcaagac ctggcggcga cactcaagaa caatcggcct tacgcggtag tcttcgacca
     7861 ccagatcacc gggccgcgca acgaggacga gttgcgcgac ttgcgcaccc ggattccagc
     7921 cggaatcccc accgtcgttt tcaccattga tgccgaaggg aagtttgggt tcatccgctt
     7981 cgcggcggtg cgaaggcccg aagcgttgac ccggccccgg ctcattgacg cgctcggccg
     8041 aaccagcaac tcttcgggca aagaggtcaa gacggtcttg attatcgaag acgaacccgc
     8101 cttgctggaa ttgctcacca agacgctgct tttcaaaggt ttccaggtgc ttcccgccgt
     8161 caacggccgc tgcggcatcg aattcgccgt tggctcccat ccggatgtga tcatcctgga
     8221 cctcacctta cccgaccgca gcgggattca agtcgttgag gacctccgcg cccgccccga
     8281 aaccaaaagc atccccattc tcatccatac cggcacggtc ctgagtgagc cggagcggca
     8341 acggctggcc gctcaggtcc agtccattac ttccaaggcc gagccgcaaa gtctgctcac
     8401 ccagctcgaa cacctggacg aagcgcccgc ggaggcagtg ctccaggagt gaacccatga
     8461 aaaagcgaat cctggtggtg gaggacaacc tgctgaacct ggagttggtc actgacctgc
     8521 tcgaagtcgg cggctttatc gtctgccccg cccgcactgg cgaagaaggc ttacatgccg
     8581 cccgcgagca gccgccggac ctcattctca tggacctcag cctgcccggt ctggacggct
     8641 tggccgccac tcgatccctc cgggccgatc ccgccacccg tcacctgacc atcatcgctc
     8701 tcaccgccca tgccatgaat ggcgacgaag ccctcgcgtt gcgcgcgggc tgcgatggct
     8761 atttgaccaa gcccatagat accagaacct tcgcggccaa agtggccgca tttatcgccg
     8821 ccgccgactc gcggctcacc caaccacagg ccctctgcca atatgcaagc tgaaaccttg
     8881 attcgtaata aatcctccgc cgcgcgcgcc gtgggctctc gcggacttgt gctggtcgtg
     8941 gacgacgagg aagccaaccg catgctgctg cgtgatccgc tggaagtcca cggctatgag
     9001 attatcgaag ccgagaacgg cgagcaggcc ttgcagaaag tcgaacaaca gcctcccgac
     9061 gtcatcctgc tggacgtgat gatgccccga atggacggtt tcgaggtctg ccgacggctg
     9121 aaaaaggaag cccgcacggc ccatatcccg atcctgattg tcaccgcgct ctccgagcgg
     9181 atggagcgca tgatgggcat cgcggccggg gctagcgatt tcctgagcaa accggtcgat
     9241 ttgcaggaac tcaccctgcg cgtcaaccac gccgtacacg ccaaacgcct cgttgaccag
     9301 ctccaggccg aacaggtgaa ggtcgaacgc ctgctcctca acagtcttcc cggccccatc
     9361 gccgagcgaa tgaaacaggg ggaagtcaac atcgccgaac atcatcccga cgtgagtgtg
     9421 ctgctggccg atctggtggg tttcacgacg ctcgccgccc acatcggccc ggagcaggtc
     9481 gtctatctgc tcaacgagat cttctccggc ttcgacttgc tcgctctcaa gcatggactg
     9541 gagaagatca agaccatcgg cgacgcctac atggctgccg gtggcgttcc gttgccgcgc
     9601 cccgaccatg ccgaagccat tgcggaactg gccctcgaca tgatggcaga gatcgaaaag
     9661 ttcaaccgcg cttacaatac ttctatccag ctccgcattg gcatcagcac cggctccgtc
     9721 gtcgcgggcg tgatcgggcg ctggaaattt acctatgatc tttggggcga caccgtcaac
     9781 ctcgcctgcc ggctggagtc gctcggccag gcgggaacca tcctggtctc cgacctgacc
     9841 tatcaacggc tgagagacaa ataccgcttc gacgacagcc gcagcctcga aatcaagggg
     9901 cgcgccgcag aactagtcca cacccttcgc ggacggctct gatcccccat atttcgcagg
     9961 cgaatgaagg gcacaataca ccgcgcgcta ccaaccgccg tttttggcga cccgccctca
    10021 acaccttccc cgacggaaag attgacgctc acgctgccat ttaaggacat ttctccttct
    10081 ccccatctgg aatgacccct caggccggca aaaaatgcga agggtatggc aattggcgcg
    10141 tagcgaaacg gcggccaaca gtggcacata ccctaggaat tgaggaatag agtaaacggc
    10201 ctggtggggt ggcgactttt cgtagggggc catctctccc ggcaccaaca caatccagaa
    10261 acgccctttg catttagagc catgaaccca agaacggcct ttaacgtccc ctgctcgcgc
    10321 ggcgagcaga ttcttcgcga cccttcccaa aacaaggacg ccgcgttcac gcacgccgaa
    10381 cgcagcaagc tgggcatcga aggtctgctg ccgtcggccg tgctcaccat ccagcagcag
    10441 gtcgccttgg aactggagca catcttccag aagcctgacc cgcttgaaca atacatcggc
    10501 ctgattgccc tgctggaccg caacgaagtt ctcttttacc ggctgatggt cgagaacctc
    10561 gatcgcctgt gcccgatcat ctataccccg acggtcggcc tcgcctgcca gcaatacagc
    10621 cacatctacc gccgcccgcg cgggttgttc ctttgccccg acgaccgcgg ccaaatcgcc
    10681 cagcgcctcc gcaacttcag ccagcccgat gtccggctca tcgtcgtcac tgacaacgaa
    10741 cgcatcctcg gcctcggcga tcaaggggcc ggtggcatgc ccatccccat cggcaaactc
    10801 gtcctgtact ccgccggcgc cggcatccat ccctccctca ccttgccgat cagcctggac
    10861 gtcggcaccg acaacagcgc gctgatcgcc gacccctatt acctgggcta ccgcggacgc
    10921 cgcctgcgcg gcccggacta cgactccttc gtcgaagagt ttgtccgcgc ggtcaagact
    10981 gtcttcccgc gcgcgctgct gcaatgggag gacttcaaga aggccaatgc tttccgcctg
    11041 ctcgagcgct acgcgggccg gctccccagc ttcaacgatg acatccaggg cacctccgct
    11101 gtcgtcctgg ccggcgtcct taccggcctg cgcctgatcg ggaagcccgt gcgcgagcaa
    11161 cgcttcctgc tggtcggctc cggcgccgcc ggcgtcggga ttggccgcct ccttcacacc
    11221 gctttgctcg ccgacggcct gactgaaacc caagcgcggc aacacgagat cttcatggac
    11281 tcgtccggcc tggtctggga agggcgcgcc aacctggaag agcacaagcg cgaggtcgcg
    11341 ctgcgcaaag acgacttggc cgccgccggc ttgtcctgtc cgccgcctga ttcgctcgag
    11401 aaactcattc gcgccgttcg gcccaccgtc atcatcggca ccaccggcca accgggcgac
    11461 ttcaccccgg aggccatccg cgcgatggcc gacaattgcg aacgccccat catccttccc
    11521 ctcagcaacc ccaccagcaa agccgagtgt acgccctccg aagccttgca gcattcggat
    11581 ggccgcgctt tggtcgccac cggctcgccc ttcgacccgg tcgtctataa aggacgcaaa
    11641 tacgtcatcg gccaatgcaa caacgccttt gtgttcccgg gcgtcggcct cggcgtcctc
    11701 atcagcgagg ccagccgcgt caccgattcc atgttcctcg ccgccgctcg cgccctggcg
    11761 gaattcacca gcacccacgc ggccagcgac aattgcctct atccgagcct gtgcgaactc
    11821 cgcaacctca gcagcctgat cgctttccgc gtcgcccgta cggcccgcga tgaaggcttc
    11881 ggcaacaacc tcgacgacaa cgccctccaa gcggcaattg acgacttctg ctggttccct
    11941 gaataccccg agaccgacca ttccgctcac catcccgcgc ctgccgctga ggctccagcc
    12001 cacactacac ccccacaaaa cctcttgtag ccctcacccg gcggcccgat gtggtccata
    12061 atgagaattg ctgtcaatcg agcggctaag cggcgttccc gctccggccc gggtccggcg
    12121 cgccggctga attcgcgggc gggggtgccg gccggggtgt tgcgcctttg cgcttttgca
    12181 gggcctgctt gagcaggtat tcgatctggc cgttgacgct gcgcagttcg gcctgcgccc
    12241 aaacctctag ctcggcccag agcacagggt caatccgcag caggaacggt ttgcgcggtc
    12301 ccatgctggc tcaggtgtag agggtgccgg tgttcagcac cggatgcact tcggactcgc
    12361 cgca
//
LOCUS       DATCGS010000327         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_275919, whole genome shotgun sequence.
ACCESSION   DATCGS010000327 DATCGS010000000
VERSION     DATCGS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275919"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..2060)
                     /locus_tag="VIL39_06695"
     CDS             complement(135..2060)
                     /locus_tag="VIL39_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5481150.1"
                     /translation="MAICNWQLAILAACLAWLVPQEAHSASLPQPGSLSPAALAATPD
                     GTRLFIACATANEVAVFDLASGAITKHIPLPHTPSGLALSSDGARLYVTCAAPQSSVR
                     VLEAGSGKTLTTLPAGHSATAPVLSPDGKTLFVCNRFNNDISVVDLASGNEQCRIPVQ
                     REPVAAAISRDGRFLLVANHLHTGPADAERVAAVISVIDTTARKVVKELSLPDGSGVL
                     NDLRVSPDGKYAVVSHILPRFRLPATQLDRGWMNSNAATLIDLARMEILNTVLLDNID
                     RGAADPWGVAWSADSATLVITHAGTHEISVIDFPAVLARLAKVPATLDPSKPVDPISA
                     SRVQADVPNDLAFLVGVRQRIKLPRNDLGPRAVVLLGRKAYVANYFSDSISLVDLGSP
                     QTNPQSIPLLLQPAGAGAKGLASPLDRMPLTRKGEFYFNDAGICFQGWQSCATCHPGQ
                     ARVDGLNWDLLNDGIGNPKNTKSLLLAHKTPPAMSMGVRETAEMAVRAGIQHILFTVQ
                     PEEVAVALDKYLKSLTPVPSPLLVNGRLSDSARRGEKLFKDRQVGCATCHPPGLFTDL
                     KTYDVGTRGSFDKPADRFDTPTLIEGWRTAPYLHDGSAATMLEVLTTRNPGDKHGKTS
                     NLTPQQLDDLAAYLLSL"
     gene            complement(2093..>2714)
                     /locus_tag="VIL39_06700"
     CDS             complement(2093..>2714)
                     /locus_tag="VIL39_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY5481151.1"
                     /translation="GKEKMIEGWVEGPVAEGADLRGINTLMLDFFDDPAFVRDLFAFV
                     VEMELRFACEQLKAGADVIGVGDAAASLVGPDIYDEFVWPYEKKLVDGIHALGGKVRL
                     HICGNTRRILEGMGKLGCDIVDLDSMAPISEARQKMGPKTVLLGNLNPVVVLRNGDPA
                     GVTAAIAECHRQAGAPFIVGAGCEVPRDTPPDNLRALCAYAHTHQP"
BASE COUNT          460 a          797 c          978 g          479 t
ORIGIN      
        1 gcgcggacat tcttgtccgc agccacgtcc gaacactcct gggctccgaa aacgtctggg
       61 gctcgcatat cgctccacgc tgctgcggac aggaatgtcc gcgcgccgct ttcctcgcgt
      121 gtcgccgaat tgcatcacaa cgaaagcagg taggccgcga ggtcgtcgag ctgctgcggc
      181 gtcaaattgg aggtcttgcc gtgcttatcg cccgggttgc gggtggtgag cacctcgagc
      241 atggtggccg ccgagccgtc gtgaaggtag ggagcggtcc gccagccttc gatgagggtc
      301 ggcgtgtcga agcggtcggc gggcttgtca aagcttccgc gcgtgccgac gtcgtaggtc
      361 ttcaggtcgg tgaacaagcc cggcggatgg caggtggcgc aaccgacctg gcggtccttg
      421 aagagcttct ccccgcgccg ggctgagtcg gagagccggc cattgacgag cagcgggctc
      481 ggcacgggcg tcagcgactt gagatactta tcaagggcca ccgcgacctc ttccggctgc
      541 acggtgaaca ggatgtgctg gatgccggcg cggacagcca tttccgccgt ttcgcgcacg
      601 cccatgctca tggcgggcgg ggtcttatgc gcgagcaaca ggctcttggt gttcttgggg
      661 ttgccgatgc cgtcgttgag gaggtcccag ttcagcccgt ccacgcgcgc ctggccgggg
      721 tggcaggtcg cgcagctttg ccagccctga aagcagatgc cggcgtcgtt gaagtagaac
      781 tcccctttgc gagtgagagg catgcggtca agtggagaag cgagcccctt tgcgccggct
      841 cctgcgggct ggaggaggag ggggatggac tgggggttgg tctgggggct gcccagatca
      901 acgaggctga tggaatcgga gaagtagttg gcgacgtagg ctttgcggcc caggaggacc
      961 accgcgcgcg ggccgaggtc attcctgggc aacttgatcc gttggcgcac gcctacgaga
     1021 aaggccaggt cgttcggcac atcggcctgg acgcgcgacg cggagatagg gtcaaccggc
     1081 ttggacggat cgagcgtggc gggcaccttg gcgagcctgg ccagcacggc agggaaatca
     1141 atcacgctga tttcatgcgt gccggcatgg gtgatgacca gagtcgcact gtccgccgac
     1201 caagcgacgc cccaggggtc ggcggcgccg cgatcaatgt tgtccagcag gacggtgttg
     1261 aggatttcca tccgcgcgag atcaatgagg gtggccgcgt tgctgttcat ccagccgcgg
     1321 tcaagctggg tcgcgggcag gcggaagcgg ggcaggatgt ggctcaccac ggcgtatttg
     1381 ccgtccgggg agacgcgcag gtcattgaga accccgctgc cgtcgggcag ggagagttcc
     1441 ttgaccacct tgcgggcggt ggtgtcaatg acgctgatga ccgcggcgac gcgctctgcg
     1501 tcagcggggc cggtgtgcag atgattcgcc acgaggagaa accgcccgtc gcggctgatc
     1561 gcggcggcca ccggctcgcg ctggaccggg atgcggcatt gctcgttccc gctcgcgaga
     1621 tccacgacac tgatgtcgtt gttaaagcgg ttgcaaacga acagggtctt gccgtcgggg
     1681 ctgagcacgg gggcagttgc actgtgaccg gcgggaagcg tggtgagcgt cttgccggag
     1741 cccgcctcga gcacacgaac gctgctctgc ggggcggcgc aagtcacgta gaggcgcgcg
     1801 ccatcggacg agagtgccaa gccggaagga gtatgaggca gggggatgtg tttggtgata
     1861 gcgccgctcg cgaggtcgaa gactgccacc tcattcgccg tggcgcaggc gatgaagagc
     1921 cgtgtgccat ccggcgtggc ggccagggcc gcgggcgaga gagaaccggg ttgcggaagc
     1981 gaggctgaat gcgcctcctg gggaacgagc caggcgaggc aagcagccag gattgctaat
     2041 tgccaattgc aaattgccaa ttgcaaattg gactttggtg gcgggttctg gatcatggtt
     2101 ggtgggtgtg ggcgtaggcg cataaggcgc ggaggttgtc cgggggcgtg tcgcggggca
     2161 cttcgcagcc ggccccgacg atgaatggcg cgccagcctg acggtggcat tcagcgatgg
     2221 cggccgtgac tcccgccggg tcgccgttgc gcagcaccac caccgggttc aggttgccga
     2281 gcaggacggt cttgggaccc atcttctggc gcgcttcgga gatcggcgcc atcgagtcca
     2341 ggtccacgat gtcgcaaccg agcttgccca tcccctccag gatgcggcgg gtgttgccgc
     2401 agatatgcag gcgcactttg ccgccgaggg cgtgaatccc gtccacgagc ttcttctcgt
     2461 agggccagac gaactcgtcg tagatgtccg ggccgaccag tgaggcggcg gcatcgccga
     2521 cgccgattac atccgcgccc gccttcaatt gctcgcacgc aaagcgcagt tccatctcga
     2581 cgacgaatgc gaacaggtcg cggacgaatg cggggtcatc aaagaaatcc agcatcaggg
     2641 tgttgatgcc ccgcaggtcc gccccttccg caacggggcc ttcgacccag ccctcgatca
     2701 tcttctcttt gccg
//
LOCUS       DATCGS010000328         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_78228, whole genome shotgun sequence.
ACCESSION   DATCGS010000328 DATCGS010000000
VERSION     DATCGS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78228"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VIL39_06705"
     CDS             <1..186
                     /locus_tag="VIL39_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481152.1"
                     /translation="EGAPFPPVSSAAALPSAAITARLQTNIRFIRTPLLEFRKGKHAA
                     AAYKHTASSTKVPDGTS"
     gene            1196..2500
                     /locus_tag="VIL39_06710"
     CDS             1196..2500
                     /locus_tag="VIL39_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5481153.1"
                     /translation="MKRNICCNLTLLLTWALGSTVALAQPTNSLPDAPSPEQRDAATL
                     EVLISEAWANNPEVRLFEAAVAGARGELVTARTWDNPQLSVTPGVFRDAGGNWGHAGF
                     EFSQTILFPGKRALQRAVAEKTVESRQLALTAFRTQLAIQVHRSFYSLLAARQVVALS
                     QQHLKLSQSFLAAARNKVEGGFAPDFEQTKAEVDVAAAMKSLRQTQAQEAVARTALNS
                     LVGRNLDATVPIAGEFSESPSVPGEATLLHHALARNPSLKIQTTEVERTGLSLALTKK
                     SRLPDFTIGPTVEYQQPEQVYGLGISLPFPLWDKKKGAITTATAAQQRALAELDTLRR
                     DIIREVSSAAHLLAAARESLAIYTPAFRGKLKSALDNASQSYAEGRTSLLVYLETERT
                     YYDTEAGYYETLQRLYDAQAALEAGLGMPLNALEKTNPPTNK"
     gene            2513..3451
                     /locus_tag="VIL39_06715"
     CDS             2513..3451
                     /locus_tag="VIL39_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5481154.1"
                     /translation="MSHRIILLCTVALLAGGLAGCEKKAEPVEKAKPESKHEENIVTL
                     TPENLKHVALKIEPVTLSSLGTALKAAGRISANLNKTAKVTSTFEGRLTKLNFDLNDR
                     VKAGEVMALVESPELLGRQLEIKAQIDGVVIDRKATTGELVDKAKEIYTISDPTQLWA
                     IAEVKERDIAAVKLGQDAAFAALAYPEEKFHGKVVLIGNQVEAGSRTVEVRIAVDNAD
                     GRLKPGMFADVEIVTTILDNVLLIPDNALQTEGDNQIVFVALDGSKFEKRTVKLGLEQ
                     SGRVQILDGVKAGENIVTEGSFILKSEMLKSQLVEE"
BASE COUNT          784 a         1151 c         1012 g          672 t
ORIGIN      
        1 gagggcgccc ccttcccgcc agtttcatcc gccgccgcgc ttccaagcgc tgccatcacc
       61 gccagactac aaaccaatat tcgtttcata aggacaccgc ttttagagtt cagaaaggga
      121 aaacacgccg ccgcggcata caagcacacc gcgtccagca caaaagtccc ggacgggacc
      181 agttaagaag ccaacgaagg agcccgcgcc aacaacgcag cgcggaggat gaattgccag
      241 ggctccagag acccaggcgg agaaggggta agttcgacgc tccccggcct ggtcaccaac
      301 tcactctgcg gcggggcagc cacaaacaag caagcgccat ccaacggcag cggaattgac
      361 agcacactat cctgggaaac ataaatgcct gactgaatgg tggtgcaaac gcaatgccct
      421 ggcgcggccg gcgcctgatc cgattgcccg ccgtcttcat cgcagcaagg cagggcggca
      481 gcgtgcgtca aactctcgat tgcgcaacgt gcgctgcagg cggaccaggc cgccagcact
      541 agcactgtca caatcaccct caaatgctgc acgccgctac actacgccac cgaccccgaa
      601 ctgcaaacct aaaccgcaaa ccgcctgcct tctcgacctg ccactcattt ccagcacact
      661 tgcagagtcg caaaagattt gattgaacat ttgcgccctt tatggcatcc attatgcgtg
      721 cggtttgtaa aaacaatgct cgctctgatg ttggcgctcg cctgggtgcc gctcatgtcg
      781 cattgcaagc tggaaaccgt ccccggcctc cagttcctcc gctgtgtcct tgaggatcaa
      841 gcccccgcga aagcacccgg ccattgcgac ggcacctcct gctgtgccgt tgagtccggc
      901 aagtatcacg ccacaggttc tcaacagatc gttcctgttt ttgccctcgc gctgctccct
      961 ttcgacgtcg ttcctgactc cgggcactca ttgccccccc aggtcagtct cggccttctg
     1021 accgccgctc cgccagatct gtccacaagc tggcaattca cctcccgcac ggcccctctg
     1081 tcccgtgcgc cttcccccct ctcttaagta gtcccgtccg ggaccgctcc tctgtcgcgg
     1141 cgtgatttca cgccgtggtt tttcccccgt tttgatttgt cggtttagaa tgactatgaa
     1201 gagaaatatc tgctgcaatt tgacgctcct gctgacctgg gcgctcggaa gcacggtggc
     1261 gctggcccaa ccaacgaatt cgctgccgga cgccccgtcg ccggagcagc gcgatgccgc
     1321 cacactggag gtgctcatct ccgaagcgtg ggccaacaac cccgaggtgc gcctgttcga
     1381 ggccgctgtc gccggtgctc gcggcgaatt ggtcaccgcg agaacgtggg ataatcccca
     1441 actctccgtc actcccggcg tatttcgcga cgcgggaggc aattggggtc acgccggatt
     1501 cgagttcagc cagaccattc ttttcccggg caaacgcgcc ttgcagcggg cggtggcgga
     1561 aaaaacggtt gaatcccgcc aattggcgct cactgctttt cggactcaac tcgccatcca
     1621 ggtgcatcgc tcattctaca gcctgctcgc agcccggcag gtagtcgcct tgagtcaaca
     1681 gcatctgaaa ctgtcccaat cattccttgc cgcggcgcga aacaaagtgg aaggcggatt
     1741 cgctccggac ttcgagcaga ccaaagccga agtggacgtg gcggcagcca tgaaatcctt
     1801 gcgccaaacc caggcccagg aagccgtcgc ccgcaccgcg ctcaattcgc tcgtgggccg
     1861 gaacctggac gcaacggtgc cgattgccgg tgagtttagc gaaagcccat ccgtgccggg
     1921 ggaagccacc ctcttgcatc atgctttggc gcggaatccc agcttgaaaa tccagacgac
     1981 ggaagtcgag cgcaccggtt tgagcctcgc gttgacgaag aagtcgcgcc tgccggattt
     2041 caccatcggc ccgacggtag aataccagca gcccgagcag gtttatggat tagggatctc
     2101 cttgcccttc ccgctttggg acaagaaaaa gggcgcaatc accacggcga ccgccgcgca
     2161 acaacgcgcg ctggccgaac tcgacacgtt gcgccgggac atcatccgcg aagtgagcag
     2221 cgcggcgcat ttgctggccg ccgcgcggga atcactggcc atctacacgc cggcctttcg
     2281 cggcaaactt aaatccgcgc tcgataacgc ctcgcaaagt tacgcggaag gccgcacttc
     2341 gctgcttgtt tatctggaaa cggagcgcac ctattacgac accgaagccg gctattacga
     2401 aactttgcag cgactctacg acgcccaggc cgccctggag gccggcttgg gcatgccctt
     2461 gaatgcactg gaaaagacca acccaccaac aaacaagtaa ccgaaagaca atatgagcca
     2521 tcgaatcatc ttactatgca ctgtcgcgct gctggccgga gggctggcgg gttgcgaaaa
     2581 aaaagccgaa cccgttgaaa aggcgaagcc ggaatccaaa cacgaggaaa atatcgtcac
     2641 cctcacgccg gagaacttga agcatgtcgc attgaagatt gaacccgtga cgttgagcag
     2701 tctcggcacg gccctcaagg cggccgggcg catcagtgcc aacttgaaca agactgccaa
     2761 ggtcacctcg acgtttgaag gccggctgac caaactcaac ttcgacctga acgaccgcgt
     2821 gaaggcgggc gaggtgatgg ctctggtcga gtcgccggaa ttgctcggcc ggcagcttga
     2881 gatcaaagcg cagattgatg gcgtggtcat cgatcgcaag gccaccaccg gcgagttggt
     2941 ggacaaggcc aaggaaatct acacgatcag cgacccgact cagctttggg ccatcgccga
     3001 agtgaaggag cgcgacatcg ccgcggtaaa gctcggtcag gacgcggctt tcgcggcgct
     3061 ggcgtatcct gaagagaagt ttcacggcaa ggtcgtgctc attggcaacc aggtggaggc
     3121 cggttcgcgt acggtcgagg tccgcatcgc cgtggacaac gctgacgggc gtttgaagcc
     3181 gggcatgttt gccgacgtgg agatcgtcac tacgattctc gacaacgtgt tgctcatccc
     3241 agacaacgcg ctgcaaaccg aaggtgacaa ccaaatcgtt ttcgtcgcgc tcgatgggag
     3301 caagtttgag aaacgcacgg tcaaactcgg tctggagcaa tcgggccgtg tgcagattct
     3361 cgacggcgtg aaggcgggtg aaaacatcgt caccgagggc agcttcatcc tgaagtcgga
     3421 aatgctcaaa agccagttgg ttgaggaata gcgtcatgat caaccacatc ctcaatttct
     3481 ccgtccgtca gcgattgctc gtcctgctgg cgacgctgat cctcgctgga ttcggctggc
     3541 tctcgctccg gcaaatcccg attgacgcct ttcctgacgt gaccaatgtc caggtgcagg
     3601 tgctcgcgac cgccggcgg
//
LOCUS       DATCGS010000329         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_622993, whole genome shotgun sequence.
ACCESSION   DATCGS010000329 DATCGS010000000
VERSION     DATCGS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622993"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..2269
                     /locus_tag="VIL39_06720"
     CDS             431..2269
                     /locus_tag="VIL39_06720"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5481155.1"
                     /translation="MNDIDIESLKGVAQQLRIHSIEATTAAGSGHPTSCCSAADVVAA
                     LFFGHMRYDPKNPHFHNNDRFILSKGHAAPLLYAAWAETGLFPVEELLKLRQFGNDLE
                     GHPTPRLPFVDVATGSLGQGLAVGVGMALCAQLDNLDYRTYVLLGDGECAEGAVWEAA
                     SLAGTYHLNNLVAVVDVNRLGQSQPTALSGDFGAYKRRFEAFGWRTEEIDGHDFEEIT
                     EVLAAVGLGAQPLVILAKTMKGAGVSFLQDKEGWHGKPLSKEEADRAIAELRPSAKTG
                     IGLPIPAPSSLPAPVNAAPASYPPITYKLGDMVATREAFGSALARIGGADLRVVAMDG
                     DTKNSTYSDKFYKKFPQRFTECFIAEQCMVGVATGFATRGKVPFASTFAAFFTRAYDQ
                     IRVAGVSMANIKLVGSHVGVSIGEDGPSQMGLEDIAMMRAIIGSSVLYASDAVCAEKL
                     VEQMATQKGVCFLRTSRPKTPVLYNNDEQFPIGGAKVLRQSAEDKVTVVAAGVTLYEA
                     LKAADALKGEGIGITVIDAYSIKPLGKDVIKAAAKKTKNLVVTVEDHYAEGGLGDAVA
                     GELSAEGVKVHKLAVRELPRSGKPAELMAKYGIDAAAIVAKVKALV"
     gene            2450..3010
                     /locus_tag="VIL39_06725"
     CDS             2450..3010
                     /locus_tag="VIL39_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5481156.1"
                     /translation="MAEKDTFLKGQLLLDSGQLSGSFFQHTVVLICQHDAEGAFGLVL
                     NRAMGKSVSDMIVADLPEALKECSLYLGGPVQPSALSFLHSDALLPDANVMPNLSLSH
                     SIDELVELGEAFSPTRKLKMFAGYAGWSPGQLENEMKHKAWLTHPASLELVFDTAPEQ
                     LWQGILRLKGGKYRLLAQAPDDLSLN"
BASE COUNT          590 a          867 c         1043 g          562 t
ORIGIN      
        1 gcagtgaact ttgcaacttc atgtcgcgac ccaaggccac cccaaggcta caccaaagcc
       61 accccaaggc tacaccaagg ccacctgaag gctcccttga tgccagagga gaatctgggt
      121 aaggcaaggg cttagtgagg cgcgttggcg agccttggtg gtgcgctaga attgctcagg
      181 tcagccccaa atccgaagtc cgaagcccga agccactagc cctcccctgt gggcgcatcc
      241 tgatacgacc accaaggcgg gcggctatgc caatcggaac gccgggttca tccggcagtc
      301 ccagtgcggc tcgccggcct gccggatgaa tccggcgttc cggtggtggt gtcaggatgc
      361 gcccggctcg ggtttggccg ggcatttctc ctttcttttt ggcattctgc gcttacagtt
      421 gaaagtatcc atgaacgata ttgatattga gagcttgaag ggggtcgcgc agcaattgcg
      481 gattcattcg attgaggcga cgacggcggc ggggagcggg catcctactt cgtgttgttc
      541 ggcggccgac gtggtggcgg ccctgttctt cgggcacatg cgttacgatc cgaagaaccc
      601 ccattttcac aacaacgacc ggttcattct ctcgaaaggc cacgccgcac cgttgctgta
      661 cgcggcttgg gcggagacgg ggctgtttcc ggtggaggag ttgctcaagc tccgccagtt
      721 cggcaacgac ctggaaggcc atccaacgcc gcgtctgccg tttgtggatg tggcgacggg
      781 gtcgctgggc cagggattgg ccgtgggggt gggcatggcg ttgtgcgcgc agttggacaa
      841 cctggactac cggacttatg tcctgctcgg cgacggcgag tgcgccgagg gggcggtgtg
      901 ggaagcggca tcgctggcag gcacctacca tctgaacaac ctcgtcgcgg ttgttgatgt
      961 aaaccggctg ggccagagcc agcccacggc tctgagcggc gactttggcg cctacaagcg
     1021 gcgctttgag gctttcggct ggcgcacgga ggagattgac ggccatgact ttgaggaaat
     1081 caccgaagtg ctggccgccg tgggcctggg cgcgcagccg ctggtgatcc tggccaagac
     1141 tatgaaggga gccggcgtct ctttcctgca ggacaaggaa gggtggcacg gcaaaccgct
     1201 gtcgaaggaa gaggccgacc gtgcgatcgc ggaactgcgg cccagcgcaa aaaccgggat
     1261 tggcctgccg attcccgccc cgagctcgct gccggcgccg gtcaacgccg cgcccgcttc
     1321 ctacccgccc ataacctaca aactcggcga catggtcgcg acgcgcgagg cgttcggcag
     1381 cgccctggcg cgcatcggtg gagcggacct gcgcgtggta gcaatggatg gcgacacgaa
     1441 gaactccacc tattcggaca agttttacaa gaagttcccg cagcgtttca cggaatgctt
     1501 cattgcggag cagtgcatgg tcggcgtggc gacgggcttc gccacgcgcg gcaaggtgcc
     1561 gtttgcctcg acgttcgccg ctttcttcac gcgggcctac gaccagattc gggtggccgg
     1621 ggtgtccatg gcgaacatca agctggtcgg ctcgcacgtg ggcgtgagca tcggcgaaga
     1681 cgggccgtcg caaatgggtt tggaagacat cgccatgatg cgggcgatca tcggcagctc
     1741 ggtgttgtat gccagcgacg ccgtgtgcgc cgagaaactg gtggagcaga tggccaccca
     1801 gaagggcgtt tgcttcctgc ggacgtcgcg tcccaagacg ccggtgctct acaacaatga
     1861 cgagcaattc ccgatcggcg gcgcgaaggt gctgcgccag tcggcggaag acaaggttac
     1921 ggtggtggct gcgggggtca ctctctatga ggcgctaaag gcggcggacg cgctgaaggg
     1981 cgaggggatc gggattacgg tcattgacgc ctacagcatc aagccgctgg gcaaagacgt
     2041 gatcaaagcc gcggcgaaga agacgaagaa cctggtggtg acggtggagg accattacgc
     2101 cgaaggcggg ctgggcgacg cggtggcggg cgaattgagc gcggagggcg tgaaagtcca
     2161 taagctggcc gtgcgggagc tgccgcgctc gggcaagccg gcggagctga tggcgaagta
     2221 cggcattgac gccgccgcca ttgtggcgaa ggtcaaggcg ctggtttgag cagacgctca
     2281 gtcgttcggc acccagcctt tgccggggat gtaatcgcgg ctaccggacg aggtgctgca
     2341 cccagtcgcc acgagcaggg cgaccaaggc taagagcaga caaagttttg acatagggta
     2401 aatttaatcc agattggcta tacggcaagc ttgagatggt tttagcgata tggcggagaa
     2461 ggacacattc ttgaagggcc agttgctgct cgacagcggc caattgagcg gctcgttctt
     2521 ccagcacacg gtcgtcctga tctgccagca tgacgcggag ggggcgttcg gcctggtgct
     2581 caaccgcgcc atgggcaaga gcgtcagcga catgatcgtc gccgatctgc cggaagcgct
     2641 taaggaatgc tcgttgtact tgggcgggcc ggtccagccg tcggcgctga gttttctcca
     2701 ctcggatgcg ctgctgcctg acgctaacgt gatgccgaac ctgagcctga gccattccat
     2761 tgatgagctg gtagagcttg gcgaagcgtt ctcaccgaca cggaagctca agatgttcgc
     2821 cggctacgcc ggctggagcc cgggccagct cgaaaacgag atgaagcata aggcatggct
     2881 cactcatccg gcctcgctcg aattggtttt cgacaccgcc cccgagcagc tttggcaggg
     2941 catcctgcgg ttgaaaggcg ggaaatacag gctgctggcc caggcacccg acgacttgtc
     3001 cctcaactga agcagggcag ggagtttccg attgcggcgg gggagaggat tctcagatcg
     3061 ga
//
LOCUS       DATCGS010000330         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_78367, whole genome shotgun sequence.
ACCESSION   DATCGS010000330 DATCGS010000000
VERSION     DATCGS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78367"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..317)
                     /locus_tag="VIL39_06730"
                     /pseudo
     CDS             complement(189..317)
                     /locus_tag="VIL39_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
     gene            418..792
                     /locus_tag="VIL39_06735"
     CDS             418..792
                     /locus_tag="VIL39_06735"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481157.1"
                     /translation="MKLSDALVLDARLAGDALERSIAGQVEFWARLGRAVEPLLQGAQ
                     ALALCRNAVARPLSACLDSVDSPAGRRRVAEFLRTQPYPHYEPAPDAPGLLIRIEAGG
                     KRTRGRFVNRQFQAAKPKPAKR"
     gene            789..1409
                     /locus_tag="VIL39_06740"
     CDS             789..1409
                     /locus_tag="VIL39_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEY5481158.1"
                     /translation="MSLPLDQRPIIVALAGPNGAGKTTFYHAHLQPAGLRFVNADVLA
                     RELDLDPYAAAKVADALRRQLVQQRESFVFETVFSDPVGDKLAFLKEAAKAGYAVVLC
                     FIGISGPAVSEERVAMRVSQGGHDVPAEKLIARFPRTLANLQAALRELPNALIFDNDD
                     LRTPFRQVAVFHNRQRVSLNPPIPRWLKPLLPLRTCPTASGGVSGC"
     gene            complement(1576..1848)
                     /locus_tag="VIL39_06745"
     CDS             complement(1576..1848)
                     /locus_tag="VIL39_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481159.1"
                     /translation="MRTTVTLEKDVEQMLRGAMHRSKQSFKATLNSALRAGLDAKPVP
                     GRRGRFVVKARPMGLRTGIDPASLNKLADDLEADSVIAKAQRTERA"
     gene            complement(2052..>2710)
                     /locus_tag="VIL39_06750"
     CDS             complement(2052..>2710)
                     /locus_tag="VIL39_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY5481160.1"
                     /translation="HCLADYIAPKSSQLSTINSPLPDYLGAFAVSAGFGVEDLCRKFE
                     ADDDDYNSIMTKALADRLAEAFAEYLHQRARKDWGYGLSENLTTEEIIREKYRGIRPA
                     AGYPACPDHTEKWTLWKLLDVENSTGIKLTESCAMWPGASVSGLYFAHPESKYFAVGK
                     IGRDQVLDYHQRKQMDLPAVERWLGPYLNYEPGTAASADSGNLNRSGEGVAHAKAKEG
                     "
BASE COUNT          469 a          877 c          829 g          535 t
ORIGIN      
        1 ctttggggtg gcttcacgct ggagatgacc tctggcccct gagctctgac ctctgacctc
       61 tggccgcacc agccctccac cttctgcatt cttccttctg ccttcttcct tcctttaaat
      121 cgggcgtgct cttcggcgaa ggggcgcgcg gcttcgggtt ggcccaactg ctcgtagtgc
      181 gcggcgagcc actccgccgg gtcttgctgg tagaagcgtt tcagttcgtc gtaaagctcc
      241 gggtgcttct ggcgcagtgg ccgcggcttc tcaaagaagc acgcggtggc cacggcgaag
      301 aattcggcag gattggtctc atcctaacag gattccagcg gcatcgcaag gacggtcgtg
      361 acatcgcggg attgacagtg ggtagccaaa tgctaccgta tgcttatgag tcaaccggtg
      421 aaactgtccg acgccctggt gctggatgcg cgcctggcgg gcgatgcctt ggaacgatcc
      481 atcgcggggc aagttgagtt ctgggcccgg ctcggccggg cggtcgaacc cttgctgcaa
      541 ggcgcccagg cgctggcctt gtgccgcaac gccgtcgccc gcccgctttc agcctgcctc
      601 gactcggtgg attctcccgc cggtcgccgc cgggtcgccg agttcctgcg gacccagccc
      661 tatccacact acgagccggc cccggacgcc cccggattgc tgatccgcat cgaggccggc
      721 ggcaaacgca cgcgggggcg cttcgtcaat cggcagttcc aggccgcgaa gcccaagccg
      781 gccaagcgat gagcctgccg ctggaccagc gtcccatcat cgtagcactg gcggggccaa
      841 acggcgcggg caagaccacg ttctaccacg cccatctcca acccgccggg ttgcgtttcg
      901 tcaacgccga cgtgctggcg cgggaactcg acctcgatcc ctacgctgcc gcgaaagtgg
      961 ccgacgccct ccgacgccaa ctcgtgcaac agcgcgaaag cttcgtgttc gaaaccgtct
     1021 tctccgatcc tgtgggcgac aaactggctt tcctgaagga ggcggcgaaa gccggctacg
     1081 ccgtggtgct ctgcttcatc gggatttccg ggccggccgt ctccgaagaa cgcgtcgcca
     1141 tgcgcgtttc ccagggcggc cacgacgtgc cggccgagaa actgatcgcc cgtttccccc
     1201 gtaccctggc caatctccag gccgctctcc gcgaactccc gaatgcgctg atcttcgaca
     1261 acgacgatct gcgaacccca ttccgccagg ttgccgtctt tcacaaccgt cagcgggtga
     1321 gtctgaatcc cccgataccc agatggctca agcccctgct gccgttgcgg acgtgcccga
     1381 ccgcttctgg cggcgtaagc ggatgctgaa acacccgggg gttcgtgccg acgcgcacga
     1441 acccgaacac tacaacctgc ggcagcccca ccggttcctc gcccgacaag cacgcctgcc
     1501 accacgctac ggccttcgcg tggaatgggg aagccgcatc gtaagcgtag aggagcaagt
     1561 tgatgtcggg gatgatcatg cccgctccgt ccgctgggcc ttggcgatga cgctgtccgc
     1621 ctccaggtca tccgccaact tgttcaggct ggcggggtca atccccgtcc gcaagcccat
     1681 cggtcgtgcc ttgacgacga aacggccacg gcggcctggc acgggcttcg cgtccagacc
     1741 ggcccgcaac gcactgttga gcgtcgcctt gaaggattgc ttggaccgat gcatggcgcc
     1801 acgcaacatc tgctccacgt ccttctccaa cgtcactgta gttctcatgc tggcatcatg
     1861 atgccaattc actttgttgt caagatgccc ataacggggt tgagcttgat tgacgagtgg
     1921 cgggcatcag gacaatacaa caggggctac gagcctgctg tcttcccttt ccttaattga
     1981 acaccttcag aaaaccaacg aaatcaatgg agggggtgaa ggtaatcgaa ccctcaaaac
     2041 catcctctgg ctcagccctc ctttgccttc gcgtgggcca caccttcgcc agagcggttt
     2101 aggttgccgg aatccgctga ggctgccgtg ccgggttcat agttcagata cgggccaagc
     2161 cagcgctcga cggcgggcag gtccatttgc ttgcgctggt gatagtcgag cacctggtcg
     2221 cggccgatct tgccgacggc gaagtacttc gactccgggt gggcgaagta caggccgctg
     2281 acgcttgcgc cgggccacat ggcgcagctt tcggtcagct tgatgccggt gctgttttcc
     2341 acgtcgagca gcttccagag ggtccatttc tcagtatggt cggggcaagc ggggtagccg
     2401 gcggcgggac ggatgccgcg atacttttcc cggatgatct cttcggtggt tagattctcg
     2461 gacaggccgt agccccagtc cttgcgggcg cgctggtgga gatactcggc gaacgcttcc
     2521 gcaagccgat cggcgagcgc cttggtcatg atcgagttgt aatcgtcatc gtcagcctcg
     2581 aacttgcggc ataggtcctc gacgccgaaa ccggcgctca cggcgaaagc gcccaagtag
     2641 tcggggagtg gagagttgat agttgatagt tgggaagact ttggggcgat gtaatcggcc
     2701 aggcagtggt
//
LOCUS       DATCGS010000331         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_573475, whole genome shotgun sequence.
ACCESSION   DATCGS010000331 DATCGS010000000
VERSION     DATCGS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573475"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VIL39_06755"
     CDS             <1..907
                     /locus_tag="VIL39_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5481161.1"
                     /translation="ANNAQATREQILLAVDGAFFAAQQAQSVTRVAEQTVAARQLFLD
                     QVSALATNKLRSELDVSFAKVNFDDGKLLLSKAQNDLQAAFAQLANLMGLRESKSYRL
                     VEQPMPPEVSTNVSEFVQLALQSRPDLLSLRNSQEAALKFARAEKALHYPTLSAVGSA
                     GVVPIGDSQLPNNYAAAGLSLTVPLYAGGLYSARQQEAELRARAADENLRDLENNVIR
                     DVRIAWLSAQNAFDRLRITGELLDNAKQSFDLAQARYSNSISSIVEFNQAQLNLLSAQ
                     IAYADTQYEYLLRRSALSFQTGSLH"
     gene            complement(941..1405)
                     /locus_tag="VIL39_06760"
     CDS             complement(941..1405)
                     /locus_tag="VIL39_06760"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5481162.1"
                     /translation="MNDNFVQRGGLWVFCQSVLLCAVIAGGILGRNQWHSLALTLCGA
                     GLIVIAAGCGLAGTVSLGRNLTPFPKPSAGSSLVQTGIYGFIRHPLYTAVCCGAVGWA
                     LVWRSWPALVAAMALAPLFDAKARLEERWLRQQFPEYAGYEQQVRRFVPWIY"
     gene            complement(1531..1851)
                     /locus_tag="VIL39_06765"
     CDS             complement(1531..1851)
                     /locus_tag="VIL39_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481163.1"
                     /translation="MATATEQLSWAATTAGMALHSLMGGVALAAAAGTFLCIACADLL
                     PELQFHSHDRLKLSLALAAGLGVAVLIGQFEASGDDHHQPELSLVRLCKQAVTSLCSL
                     SRGI"
     gene            complement(1835..2230)
                     /locus_tag="VIL39_06770"
     CDS             complement(1835..2230)
                     /locus_tag="VIL39_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEY5481164.1"
                     /translation="MKIALPVENGHLNSHFGGSRQFAIIEVDPNTKTTLRTETLPAPE
                     HQPGVFPRWLQSLGVELVIVGGIGQRALAIFAQHGINVVAGLPDQPVEALVAAYLNGQ
                     LTGKPEGCQHHGQGHHDHPGHEHHGGNCH"
     gene            2307..>2789
                     /locus_tag="VIL39_06775"
     CDS             2307..>2789
                     /locus_tag="VIL39_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481165.1"
                     /translation="MSLNAGWSGEESSLTPAGAAPASSAAATTAGHHKLPSLQFPSPI
                     GGENGSDLATNMWQHLDAGRFESHLEFSRNRPAHQDVHAHFNEAPDDLVRLLFKEHDF
                     PALQIGFALQENQKQARRHVEDWRHMALAVGNRNQHPFRNASFMPAPTWLADKSGSPL
                     E"
BASE COUNT          578 a          881 c          826 g          504 t
ORIGIN      
        1 agcgaataat gcgcaggcca cccgcgagca aatcctgctg gctgtcgatg gggccttctt
       61 cgcggcgcag caagctcaat cggtgacccg cgtcgcggaa cagacggtcg cggcacggca
      121 gctatttctt gaccaggtca gcgcgttggc caccaacaag cttcgctctg agctcgatgt
      181 cagtttcgct aaggtcaact tcgacgatgg caaactcctg ctgagcaagg cgcagaacga
      241 tttgcaggcc gcatttgcac aactcgccaa cctaatgggc ctgcgcgagt cgaagagcta
      301 ccgcctggtg gagcaaccga tgccgccgga ggtttcaacc aatgtctcgg agtttgttca
      361 gctcgccctc caatcccggc ccgacttgct cagcctgcga aactcgcagg aagcggccct
      421 caaattcgcc cgagcggaaa aggccctgca ttaccccacc ctctcggccg tgggcagcgc
      481 cggcgtggtt cccatcggcg attcccagtt gccgaataac tatgccgctg ccggcctgag
      541 cctgacggtg ccgctctatg ccggcggcct ctattccgcg cggcaacagg aggccgagct
      601 gcgcgcccgg gcggcggacg agaacttgcg cgacctggag aacaacgtga ttcgggatgt
      661 gcgcattgcc tggctcagcg cccaaaatgc cttcgaccgg ctgcgcatca ccggcgagct
      721 tctggacaat gccaaacagt cctttgacct cgcccaggcg cgctatagca acagcatcag
      781 ttcgatcgtg gagttcaacc aggcgcagct taacttgctt tcggcccaga tcgcctatgc
      841 ggacacccaa tacgaatacc tgctacggcg gtcagccctg agcttccaga ccggctcgtt
      901 gcactgaccc cagccgcgcc gggtattggc tggttcttcc tcagtaaatc cagggcacga
      961 accggcgcac ttgctgctcg tagccggcat actcggggaa ctgctgcctg agccaccgct
     1021 cttccagccg ggccttggca tcaaagagcg gggccagagc catcgccgcc acgagtgcgg
     1081 gccagcttcg ccaaaccagc gcccagccaa ccgcgccgca acacaccgcc gtgtaaagcg
     1141 gatggcggat gaaaccatag atgccggttt ggaccaggct gctgcccgct gagggtttag
     1201 gaaacggcgt caggtttcgg cccaggctga ccgtgcccgc cagtccgcag ccggcggcga
     1261 tcacaatcaa gcccgcgccg cagagcgtga gggcgaggct gtgccattgg ttgcgaccga
     1321 ggatgccacc cgcaatgacg gcgcacaaca ggacgctctg acagaacacc caaagtccgc
     1381 cacgttgcac aaaattgtcg ttcatgcgtg tcgcatcgct caacggttaa taactccaga
     1441 tcaagggcaa ggccaatagt tttcctcaga gcgaaaatgg aattacaaca cgtcaggtgc
     1501 ataaatcgcg agccagattg caaatcactc ctatatccct cgggataatg agcacaagga
     1561 cgtcaccgcc tgcttgcaca gccgtaccaa agatagctca ggctgatggt ggtcatcacc
     1621 agaggcctcg aactggccaa tcagaacggc gacaccgagc cccgctgcca gggccagcga
     1681 gagcttcaac cggtcatgcg agtggaactg cagttccggc aagaggtcgg cgcaggcaat
     1741 gcacaggaac gtgcctgcag cggcggccag ggccacaccg cccatgagcg agtgcaaggc
     1801 catgcccgca gtggttgcgg cccacgagag ttgttcagtg gcagttgcca ccgtggtgct
     1861 cgtggccggg gtggtcgtga tggccctgcc cgtggtgctg gcacccttcg ggcttgccgg
     1921 tcaactggcc attcaagtag gccgcaacca gtgcttcgac cggctgatcg ggaagcccgg
     1981 ccacgacgtt gatgccatgc tgggcaaaga tggcaagggc gcgctggccg atgccgccga
     2041 cgatgaccag ttcgacgccc agactttgca accagcgcgg aaacactccc ggctggtgct
     2101 cgggcgcggg cagcgtttcg gtgcgcagcg tggttttggt gttcgggtcc acctcaatga
     2161 tcgcaaactg gcggctgccg ccgaaatgcg agttcaggtg gccgttttca acagggagtg
     2221 caattttcat ggttctattg tttgtttcgt tatcgttcat taaaaaaggg gtggaatggg
     2281 gttggttggg catagacaaa tcctgaatgt cactgaacgc tggatggtcg ggagaggaat
     2341 cttctttgac acccgcaggc gccgcgccgg cctcctctgc ggcggcgaca acagccgggc
     2401 atcacaaact gccctccttg cagtttccca gcccgatagg cggcgagaac ggatccgacc
     2461 tcgccacaaa tatgtggcag caccttgatg ccggccgatt cgagagtcat ctggagttct
     2521 cgcgaaatcg ccccgcacac caggacgtcc acgcccattt caacgaggca ccggacgatc
     2581 tggtccggct gcttttcaaa gagcacgact tccctgcgct ccaaatcggc ttcgcccttc
     2641 aagagaacca gaagcaggcg cgccgccacg tcgaagactg gcgacacatg gccctggcag
     2701 ttgggaaccg caatcagcac cctttccgta acgcgagttt catgccagcc ccgacctggc
     2761 tggcagataa gtcgggatct cctttagaa
//
LOCUS       DATCGS010000332         3265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_722210, whole genome shotgun sequence.
ACCESSION   DATCGS010000332 DATCGS010000000
VERSION     DATCGS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722210"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VIL39_06780"
     CDS             <1..253
                     /locus_tag="VIL39_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481166.1"
                     /translation="TNRTEQILGPYNRLVPQLLSGNKMRVSFVGMDGTNYVLERSFKL
                     SPATWVPQATNPADSVGVLVLTNTPNPATNNFWRIHSVP"
     gene            complement(357..1367)
                     /locus_tag="VIL39_06785"
     CDS             complement(357..1367)
                     /locus_tag="VIL39_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEY5481167.1"
                     /translation="MNLIRKRACSRAGLIGNPSDGYHGKTISLIVRNFWAEVVLYEWD
                     SLEIVLAEDDRARFASIHDLARDVKLHGYYGGIRLIKATIKRFVDYCQARNIKLHDRN
                     FSIRYQTNVPRQVGMAGSSAIILATLRCLMEFYEVNIPKEVQPSFALPIEREELGITA
                     GLQDRVVQVYEGVVYMDFDLARERSVEGLPSYYYEPIDPALLPPIYLSYHTDFSEPTE
                     VFHSDIRARFNRGEAKVVNAMKHCAYLAERGREALLRRDYGQLGRLMDENFDARRSIY
                     SLPPWQLQMIEAARRCGASAQFSGSGGAIIGIYTDEAMFKRLSESLAVQHCCTIKPKV
                     LP"
     gene            complement(1351..2244)
                     /locus_tag="VIL39_06790"
     CDS             complement(1351..2244)
                     /locus_tag="VIL39_06790"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5481168.1"
                     /translation="MNEVNIAVVPVAGLGTRLLPATKSQPKEMLPVGRKPGVQYVVEE
                     LTRAGVKRLLFVTGPGKTSIENHFDLNDEMIQHLRETGKEELLEELEFERAPVQYFYT
                     RQRQLLGLGHAVLCAQPFVGDLPFVVALGDSIIGLHAQSDIVRRMARCFVEEKAEVVV
                     AFEEVPKNEVHHYGVAKPKGSGELFELEDVIEKPTAQEAPSNLAIAARYVFSPAIFDA
                     LQRTAPGKGGEIQLTDAIRLLIQEGKKVLGMRLRKNERRFDIGNYDAYFRAFVEFALA
                     DKKYGASLRKFLVKLLNEPHP"
     gene            complement(2344..>3265)
                     /locus_tag="VIL39_06795"
     CDS             complement(2344..>3265)
                     /locus_tag="VIL39_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5481169.1"
                     /translation="NFVTSLAMECQVPECKQRQPTELGRKLKRLRPWVQMGFLGVWLA
                     PVGQWLHGIPSCVFHCYSCPLSSFACPIGIMANYAALMPVALEVPYLLLGLLLLVGAV
                     TGSLACGWACPFGFLQDLLGKIPIRKVALPAWVGYTRYVVLLGLVILLPLILGFKGIP
                     YGDQVISICRLCPAGALEAGLPASTQSVMAGNGWPMSWFKTLILVAFFASALFVHRPW
                     CRIFCPLGGLLALFNRFSLFHLRFNPKGCVECNLCRSRCPMGVNVDQKVNVSGCIRCL
                     ECSTCGALEPCFAVPKESPKPTPPPAQQAT"
BASE COUNT          695 a         1032 c          949 g          589 t
ORIGIN      
        1 cactaaccgc accgagcaaa tcctgggccc ttacaatcga ctcgtccccc agctcctgag
       61 cggcaacaag atgcgcgtgt cctttgtggg catggacgga acgaattacg tgctggagcg
      121 ttccttcaaa ctgtcaccgg cgacctgggt gccgcaggcg accaaccccg ctgactcagt
      181 cggcgtgctg gtgctcacca acacacccaa cccggccacc aacaacttct ggcgcatcca
      241 ttccgtgccg tagcttcaac catttggttt ggcgcacgac aactatgccc gccctgacgg
      301 gaattccgca gggccgcagg cccgtcgtga aagggtctgg gagggtcccc gaaaacctag
      361 ggcaggacct tgggcttgat tgtgcagcag tgctgaactg ccaggctctc gctcaaccgc
      421 ttgaacatgg cttcatcggt gtagatcccg attatagctc ccccagaacc ggagaactgt
      481 gcgctggcgc cgcaacggcg tgccgcttcg atcatctgga gctgccaggg gggcaggcta
      541 tagatgctgc gccgcgcgtc gaagttctcg tccatcagcc ggcccagttg gccgtaatcg
      601 cggcgcaaca gagcttcgcg cccgcgctcg gcaaggtagg cgcagtgctt catcgcgttc
      661 acgaccttgg cctcgccacg attgaaccgg gctcggatgt cactgtggaa cacctcggtc
      721 ggctcgctga agtcggtgtg gtacgataga taaatgggcg ggagcagggc tgggtctatc
      781 ggctcataat agtaggaggg aagaccttcc acgctgcgct cgcgcgccag gtcgaaatcc
      841 atgtaaacca ccccttcata cacctggacg acgcgatcct gcaaaccggc cgtgatgccc
      901 agctcttccc gttcgatcgg gagcgcaaag ctgggctgca cttccttggg gatattgacc
      961 tcgtaaaact ccatgaggca ccgcagcgta gcgaggatga tcgcactgga gccggccatg
     1021 cccacctgcc gcgggacatt ggtctggtaa cggatcgaaa agttgcggtc gtgcagcttg
     1081 atattccggg cctggcaata atccacgaac cgtttgatgg tggccttgat caggcggatg
     1141 cccccgtagt atccgtgcaa cttcacatcc cgcgccagat catgaatcga ggcaaaccgc
     1201 gcccggtcgt cttctgccag gacgatctcc agcgagtccc actcgtagag caccacctcg
     1261 gcccagaagt tccggacgat caacgagatg gtcttgccgt gataaccgtc cgacgggttg
     1321 ccgatcagcc cggcccgcga gcaggcgcgt ttacggatga ggttcattca gcaatttaac
     1381 caggaacttg cggagtgagg ccccgtattt cttgtctgcc agcgcaaact cgacaaaggc
     1441 gcggaagtag gcgtcgtagt tgccaatatc gaagcgccgt tcattcttgc gcagccgcat
     1501 ccctaaaacc ttcttcccct cttgaatgag caggcggatg gcgtcggtta gttggatctc
     1561 cccgcctttg ccgggagcgg tgcgctgcaa cgcgtcgaag attgcgggcg agaagacgta
     1621 gcgggcggcg atcgccaggt tactcggcgc ctcttgggcg gttggtttct caatcacgtc
     1681 ttccaactca aacagttccc cactgccttt aggtttggcc acaccgtaat ggtggacctc
     1741 gttcttcggc acctcctcaa atgcgaccac cacctcggcc ttttcctcca cgaagcagcg
     1801 cgccatgcgg cggacaatgt cgctttgcgc gtgcaggcca atgatcgaat cgcccagcgc
     1861 gaccacaaac ggcaggtcgc ccacaaaggg ttgggcgcac aacacggcgt gccccaggcc
     1921 cagcaactgg cgctggcgcg tgtagaaata ttgcaccggc gcgcgctcga actcgagttc
     1981 ctccaacagc tcctctttgc ccgtctcgcg caagtgctgg atcatctcat cgttgaggtc
     2041 gaagtgattc tcaatggagg tcttgcccgg gcccgtgaca aacagcagcc gctttacgcc
     2101 cgcccgggta agttcctcga ctacgtattg cacccccggt ttgcgcccca ccgggagcat
     2161 ttccttcggc tgcgatttcg tggcgggcaa gaggcgcgtg cccagccctg ccaccggcac
     2221 cacagcaatg ttcacttcat tcattgatgc cagcaagcat atcgcgctaa cgagccaaat
     2281 caaggcgtga atcggcgcgc aacggtgcga gcagcaagaa gttgtcgaag gcagcagcga
     2341 cctttacgtc gcctgctgcg ctggcggcgg cgtgggcttc gggctctctt tcggcacggc
     2401 gaagcagggt tccagcgccc cgcaggtgct gcattccagg cagcgaatgc agccggacac
     2461 gttgaccttc tggtccacgt tcacacccat cggacaccgg ctgcggcaga ggttgcactc
     2521 gacgcacccc ttaggattaa accgcaggtg gaacaggctg aagcgattga acagtgccag
     2581 cagcccgccc aaggggcaaa agatgcggca ccagggacgg tgaacaaaga gcgccgaagc
     2641 aaagaaggcc accaagatca gcgttttgaa ccaactcatc ggccagccat tgcccgccat
     2701 gacactctgg gtagaagctg gcaggccggc ttccagcgcg cccgcaggac aaagccggca
     2761 gatggaaata acctggtcgc cgtagggaat gcctttgaat ccaaggatca gcggcagaag
     2821 aatcacgaga cccagcagca cgacgtaacg ggtgtaacct acccaggcag gcaaggccac
     2881 tttccggatc gggatcttgc ccaacaaatc ctgcaggaac ccgaacggac aggcccagcc
     2941 acaagcgagc gacccggtca ccgcgccgac gagcagcagc agaccgagca ggaggtatgg
     3001 cacttccagc gcaacgggca tcagcgcggc gtaattggcc atgataccga tcggacaggc
     3061 gaacgaggaa agcggacacg aatagcagtg gaagacacag cttgggatgc cgtgcagcca
     3121 ctggcccacc ggcgccaacc acacccccag gaaccccatc tgcacccaag gacgcaaccg
     3181 cttgagcttg cgcccgagct cagttggctg gcgctgctta cactctggca cctggcattc
     3241 catagctagc gaagttacaa agtta
//
LOCUS       DATCGS010000333         6306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_475322, whole genome shotgun sequence.
ACCESSION   DATCGS010000333 DATCGS010000000
VERSION     DATCGS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475322"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VIL39_06800"
     CDS             <1..1778
                     /locus_tag="VIL39_06800"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5481170.1"
                     /translation="ATKYYPLAAQWDNIAITKAANFLPPKTGGNLRPLVSLTNPPDGT
                     SALLGTIPAILPLTASAMDYDGTIAKVEFYADATRLGQATAIPYTLQWTNPPSGSYQL
                     TAVATDNGGATTVSAPVAVSILTAPQPVLLAIARLGTNLQVSWPTSATAGSLQLSSGS
                     LAPANWRLLTNSPAVSNDQYSVTVSNAGGQQFFRLGAEVDPGTLSHKLMMGYQGWFSC
                     ANDGSAQSQWRHWSSSQTPVATNLAVDFWPDVSELGSNELFSTGMTMPDGSPAKVFSA
                     YNQATVVRHFKWMKDNHLDGVFLQRFGSELSSSTLFAFRNKVTENVRLGAETYGRVFA
                     VMYDISGMSANTLVSNLTNDWLYLVNTMGITNSPAYVRHKGKPVVTVWGFGFTDRPGT
                     PQDAQTVIGFFKSAGCTVMGGVPTYWRTLTSDSQTNAAWAMAYRSFDIISPWSVGRYS
                     TTNQVDSFKANLIIPDLTDTRSHGLDYMPVIFPGFSWHNLNGGTLNQIPRYGGAFYWR
                     QAYDAIGAGCTMLYGAMFDEMNEGTSMLKMAATTNQLPVGASLVPLNIDGRPLPSDWY
                     LQLADQAGKMLRGDIPLRPQMPITP"
     gene            1994..2704
                     /locus_tag="VIL39_06805"
     CDS             1994..2704
                     /locus_tag="VIL39_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481171.1"
                     /translation="MRPPKAAFTLIELLVVIAIIAILAALLLPALASAQEKGKRIACL
                     SNLKQVGLGLTLYTDTYQEKMPSAITYGSTPGDPSTAPNTVQYTDTYGGVAKALSVGN
                     FKVFWCPSDKFDKLTHSTPLDSDYTSYRYRFVVWDNTVRFPGLKTSDFVKPVGQIVYH
                     ENTDNHYKRLQSSYITTQPTLNAIYADFHAALWKVLFRQNKPGNYYDPNWFSYGPNGV
                     LNTDKPNIGGDVHKGWDN"
     gene            complement(2828..4834)
                     /locus_tag="VIL39_06810"
     CDS             complement(2828..4834)
                     /locus_tag="VIL39_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HEY5481172.1"
                     /translation="MSHELNKPTGTPDAPKRAVLDADLDLPIQGFKGTPEEIERQWFE
                     KIYTGRGDTQKQLTLRAVLMGGVLGMFMSISNLYTTLKLSWAFGVAITACVLSYVIWN
                     GLRALSGGRLSQMSILENNCMQSTASAAGYSTGGTIGTAFGALLLIEGMHRPWYVIAP
                     FALFTAALGVFLAIPMKRQMINQEQLKFPSGIAAAETLRSLYSQGVEAMKKAYALVIS
                     LAVGGLVGLLRSYGALAEQFRHSGRPQVWLEKLQGLLYIPEALNFPQWLNPIPRGQMA
                     GLVFEPSVLLIGAGMITGLRVSLSMLLGSALLYFVVAPQLVVLDLTHARLAGYVPSFV
                     TQPGGDFNPVRWALWGGTAIMVFSSLATVALEWRTLARAFSLFKKADRSAHSAAMDAI
                     EVPMSWLVVGLIPITIGMVVVQYLAFHISIPLGIIAVALAFVVSLVCCRATGETDTTP
                     IGPMGKVTQLLYAVLPGAKGVASINLMAAGATSAAGGAAADLLTDLKSGYILGANPRK
                     QFIAQFVGIFFGTLAIVPAWYAMVPTKEALEAMNPPATLMWKAVADLLTQGIHMLPVT
                     AIWAIVIGAILGVALPLTAKLFPKVQPYLPSAMGLGLAWVMPFSNSLAFAIGAVIVAA
                     WNRLNKKGSDLYYVPVASGLIAGESLIAALIAIACTVVGLLAVR"
     gene            complement(4885..>6306)
                     /locus_tag="VIL39_06815"
     CDS             complement(4885..>6306)
                     /locus_tag="VIL39_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5481173.1"
                     /translation="AKTITIEPVTRIEGHAKVTIRLDDAGKVTQARFHVNEFRGFEKF
                     CEGRLFYEMPLITERICGICPVSHHLVSAKACDEILGVEPPRPARLLRELMHMGQTIQ
                     SHSMHFFELAGPDLILGFDADPAIRNVVGLYQADPKLTVKAIALRKFGQEIIERLGER
                     RIHPNFAVPGGVNKALSVADRDAILAGIETMIGYAQEGLALMKDWMDKHANEMETFAS
                     FPTSYLGMVTEDNAVALYEGALRMVDAQRRPIERFDGRQYLAYIAEHVEDWSYLKFPY
                     YRKLGWPGGAYRVGPLGRLNAAERMSTPLAQEEFELFRRLRKGKTVEGSLYYHYARLI
                     ENLYAIERARQLLEEPDILSTEIRTTARPSNREGVGVIEAPRGTLWHHYWVDEHGRLE
                     KVNLIVATGNNNYAMSRAVEEVAMQYVHGEPVKEGALNRIEAAIRAYDPCLSCSTHAV
                     GQMPLLVQFIAPDGSVLSEVTRT"
BASE COUNT         1270 a         1999 c         1864 g         1173 t
ORIGIN      
        1 ccgccacgaa atattacccg ctggcggccc agtgggataa cattgcgatc accaaggccg
       61 ccaacttcct gcctccgaaa accggcggca atcttcggcc cctggtcagc ctaaccaatc
      121 cgcccgacgg gaccagcgcc ctgctgggaa ccattcctgc cattctgccg ctgaccgctt
      181 ccgccatgga ttacgacggc acgatcgcta aagtcgagtt ctacgccgat gcgacccgtt
      241 tagggcaggc taccgccatc ccttacaccc tccagtggac caacccaccc tccggttcct
      301 atcagttgac ggcagtggcg acggacaacg gcggcgcgac aacggtctct gcgcccgtgg
      361 ccgtttccat tctcaccgcg ccgcaaccgg tgctgctggc cattgcccgg ctgggaacca
      421 acctccaggt ttcctggccc acctcggcta ccgccggctc acttcaactg tcctcgggct
      481 cactcgcgcc ggccaattgg cggctcctca ccaatagccc cgccgtcagc aacgaccaat
      541 acagcgtcac cgtttccaat gccggcggac agcagttctt ccggctcgga gccgaagtgg
      601 accccggcac cttgagccac aaactcatga tgggctacca gggctggttt tcctgcgcca
      661 acgacggctc cgcccagagc caatggagac attggtccag cagccagacc ccggtggcca
      721 ccaacctcgc cgtggatttc tggcccgatg tctccgagtt aggttccaat gagcttttca
      781 gcaccggcat gaccatgccc gacggcagcc cggccaaggt cttttccgcc tacaaccagg
      841 cgacggtggt gcgccacttc aagtggatga aggacaacca cctggatggg gtctttttgc
      901 agcgattcgg ctccgagtta tccagctcca cgctgttcgc ctttcgcaac aaggtgaccg
      961 aaaacgtccg cctgggcgcg gagacttatg gccgggtttt tgccgtcatg tatgacatct
     1021 ccggcatgtc ggccaacacg ctggtcagca acttgaccaa cgactggctt tacctggtca
     1081 acactatggg gatcaccaac agccccgcct acgtccgcca caaaggcaaa cccgtggtga
     1141 cggtctgggg ttttggtttc accgaccgcc caggcacgcc gcaggatgcc caaaccgtca
     1201 tcggcttctt caaatcggcc ggctgcaccg tcatgggcgg cgtgccgact tattggcgca
     1261 ctctgaccag cgattcgcag accaacgccg cttgggccat ggcctaccgt tccttcgata
     1321 ttatcagtcc ctggtcggtt ggccgttact ccaccaccaa ccaggttgac agcttcaagg
     1381 ccaacctgat catcccggat ctaactgaca cccgctcgca cggcctggac tatatgccgg
     1441 tgatctttcc cgggttctcg tggcacaacc tgaatggagg cacgctcaac caaatccccc
     1501 gctacggcgg cgccttctat tggcgccagg cctatgacgc gattggcgcc ggctgcacca
     1561 tgctctacgg tgcgatgttc gatgaaatga acgagggcac ctccatgctc aaaatggccg
     1621 ccaccaccaa ccagctcccg gtcggcgctt cactcgtgcc gctgaacatt gacggccgac
     1681 cactccccag cgactggtat ctccaactgg cagatcaagc gggcaaaatg ctccgcggcg
     1741 acatccccct gcgtccgcaa atgccgatta cgccgtagcc ctaatgtcta tgcgaaattc
     1801 aagccccgcc tctcgtagcg atgcactgaa ggcagccgtg cggcttcggc ctaagggtag
     1861 ggtcgccccg tggggccggg tcgaaccagt gacgcctgag actgtgaggg cggaaacttg
     1921 gatatttgac gtgccgacaa ctgcggcgca cgagacgcct cgcgagagct ccgttaggag
     1981 cggcacggag aacatgcgcc cgcctaaagc cgccttcacc ctaatcgagc tgctggtggt
     2041 cattgcgatc attgccattc tggcggcgct gcttttgccg gctttggcga gcgcgcagga
     2101 gaagggcaaa cgcatcgcct gcctaagcaa cttgaaacaa gtcggcttgg ggttgacgct
     2161 ctacaccgac acctaccagg agaaaatgcc ttcagccatc acctacggct caacgcccgg
     2221 cgaccccagc accgccccca atacggtcca gtacaccgat acctatggcg gagtggccaa
     2281 agcgctcagc gtcgggaatt tcaaggtctt ttggtgcccc agcgacaaat tcgataagct
     2341 gacccactcg acgccgctgg attctgatta cacctcctac cgctaccgct ttgttgtttg
     2401 ggacaacacg gtccggttcc cgggccttaa gacctccgat ttcgtcaagc ccgtcgggca
     2461 gattgtctat catgagaaca cggataacca ctacaagcgc ctgcagagct cgtacatcac
     2521 cacccaaccg acgctaaacg ccatttatgc cgacttccac gccgccttgt ggaaagtcct
     2581 cttccgccag aacaagcccg gcaactacta cgaccccaat tggtttagct atgggccaaa
     2641 cggcgtcttg aataccgaca aacccaacat cggcggcgac gtgcataaag gttgggacaa
     2701 ttaggtgggt tcattatgag cacgacctca atccgcggga atttgtcagg acgctgctgg
     2761 ccggcaccgc gggcctgagc cagagacttc tgctccggtg ttgttacggt atctcaggca
     2821 tcacgcctca ccgcaccgcc agcaggccga ccaccgtgca ggcgatggcg atgagggcgg
     2881 cgatgaggga ttcgccggca atcagccccg aggcgaccgg gacgtagtaa aggtccgagc
     2941 ccttcttgtt caaccggttc caggcagcca caattaccgc gccgatggcg aaggcaaggc
     3001 tgtttgagaa gggcatcacc cacgccagcc ccaagcccat agccgagggc agataaggct
     3061 ggaccttggg gaaaagcttt gccgtcaggg gcagtgccac acccaggatt gcgccgatga
     3121 cgatggccca aatggccgtg accggcagca tgtgaatccc ctgcgtgagc aaatcggcta
     3181 cggccttcca catcagggtg gcgggcggat tcatggcttc cagcgcctcc ttggtgggca
     3241 ccatcgcata ccatgcgggt acaatggcca gcgtgccaaa gaagatgccg acaaactgtg
     3301 cgatgaactg tttgcgcggg ttcgcgccca ggatgtagcc gcttttcaag tcagtgagca
     3361 aatcagccgc tgcgccgccc gccgcggagg tcgcgcccgc ggccatcagg ttgatcgagg
     3421 caactccctt ggcgcccggc aataccgcgt acagcaactg ggtcaccttg cccatcgggc
     3481 cgatcggagt ggtatccgtc tcgccggtcg cgcggcagca gaccagcgaa accacaaacg
     3541 ccagggcgac cgcaatgatt ccgagaggga tcgaaatgtg aaaggccagg tattggacga
     3601 cgaccatgcc aatggtgatg gggatgagcc ccacgaccag ccaggacatc ggcacttcaa
     3661 tggcgtccat agcggcgctg tgggcggaac gatcggcctt cttgaacagg gagaaggcgc
     3721 gggccagagt tcgccattcc agggcaaccg ttgccagact ggaaaagacc atgatcgcgg
     3781 tgccgcccca caaagcccag cggacgggat taaaatcgcc gccgggttga gtaacgaacg
     3841 agggcacgta gcccgccagc ctggcgtgag tcaggtccag gactaccaac tgcggagcga
     3901 ccacaaagta gagcagcgcc gagcccagca acatggagag cgaaacgcgc agacccgtaa
     3961 tcatgcccgc gccgatcagg agcacgctgg gctcgaacac cagccccgcc atttgaccgc
     4021 gcggaatcgg attgagccat tgggggaagt tgagcgcctc cgggatgtag agcaggcctt
     4081 gcagcttctc cagccacacc tggggccggc cactgtgccg gaactgttca gccagggcgc
     4141 cgtagctgcg cagcagaccc accagcccgc ccaccgccag cgagatgacc aaagcgtaag
     4201 ccttcttcat cgcttccacc ccttgggagt acaggctgcg gagcgtctcg gcggcggcga
     4261 tgccgctggg gaacttcaat tgctcctgat tgatcatctg gcgcttcatc gggatggcca
     4321 gaaagacgcc cagggcggct gtgaacaggg caaagggggc gatgacatac catggccgat
     4381 gcatgccttc gatcagcagg agggcgccga aggccgtgcc aatcgtgccg cccgtggaat
     4441 agccggcggc agaggcggtg gactgcatgc agttgttttc gaggatggac atctgactca
     4501 accgcccccc cgaaagcgcg cgcaagccat tccagatcac ataggataag acgcacgccg
     4561 tgatggccac gccaaaggcc caactaagct tgagcgtcgt gtagaggttc gagatggaca
     4621 tgaacatgcc cagcaccccg cccatcagca cggcgcgcag ggtcaattgc ttctgggtgt
     4681 cgccgcggcc ggtatagatc ttctcgaacc attgccgctc gatttcctcg ggcgtgccct
     4741 tgaagccctg tatcggcagg tcaagatcgg cgtccaaaac tgccctcttc ggggcgtcag
     4801 gagtccccgt cggcttgttc aactcatggc tcatccgcgc gattgttggg tgccacaggc
     4861 ctctgcgcga ggtttatctc acacttaggt ccgggtgact tcgcttagca ccgagccgtc
     4921 aggtgcaatg aactggacca acagcggcat ctggcccacg gcgtgggtgg agcaactcaa
     4981 gcagggatcg taggcgcgaa tggcggcctc gatgcggttg agcgcgcctt cctttaccgg
     5041 ttcgccgtgg acatattgca tggccacttc ctcgacggcg cggctcatgg cgtagttgtt
     5101 gttgccggtg gcgacgatga ggttgacctt ttccagccgg ccatgttcgt ccacccagta
     5161 atgatgccag agagtgccgc gcggggcttc gatgacgccg acgccttcac ggttggaggg
     5221 cctggcggtc gtgcggattt cggtagagag tatgtcaggc tcttcgagca gttggcgggc
     5281 gcgctcaatg gcgtagagat tctcaatgag ccgggcgtag tgataataga gcgagccttc
     5341 gacggttttg cccttgcgca aacggcggaa cagctcgaat tcctcttggg ccagcggcgt
     5401 ggacattctc tcggcggcgt tcaatcggcc aagcgggccg acgcgatacg ctccaccggg
     5461 ccagccgagt ttgcggtaat acggaaattt caaatagctc cagtcctcga cgtgctcggc
     5521 gatgtaggcg aggtactggc ggccatcgaa ccgctcgatg ggacggcgct gggcatccac
     5581 catccgcaac gcgccttcgt aaagggcgac ggcattgtcc tccgtgacca tgccgaggta
     5641 gctggtcggg aatgacgcga aggtttccat ctcgttcgcg tgcttgtcca tccagtcttt
     5701 catgagggcg agaccctcct gcgcgtagcc aatcatcgtc tcgatgccag cgaggatcgc
     5761 gtcgcggtcg gcgacggaca acgccttgtt gacgccgccg ggcacggcga agttggggtg
     5821 gatgcgcctt tcgccgagcc gctcgatgat ttcctggccg aatttccgca gcgcaatggc
     5881 cttcacggtg agtttcggat ctgcctggta taggccgacg acattgcgga tggcagggtc
     5941 ggcatcgaaa cccaggatca aatccggccc ggccagttca aagaagtgca tcgagtgcga
     6001 ctggatagtc tggcccatgt gcatcaattc gcggagcaac cgcgcggggc gcggcggctc
     6061 gaccccgagg atttcgtcgc acgcttttgc cgagacgagg tggtggctga cggggcagat
     6121 gccgcaaatg cgttcggtga tcagcggcat ttcgtaaaac aaccggcctt cgcagaactt
     6181 ctcgaagccg cggaattcat tcacgtgaaa acgcgcctgg gtgaccttcc cggcgtcgtc
     6241 gaggcggatg gtgaccttcg cgtgcccttc gatgcgcgtt acgggttcga tggtaatagt
     6301 tttggc
//
LOCUS       DATCGS010000334         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_524907, whole genome shotgun sequence.
ACCESSION   DATCGS010000334 DATCGS010000000
VERSION     DATCGS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524907"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..461
                     /locus_tag="VIL39_06820"
     CDS             258..461
                     /locus_tag="VIL39_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5481174.1"
                     /translation="MKEKGTVKWFNAAKGYGFIQRENGEDVFVHFSAIQTEGYRTLDE
                     GSRVEFEVTKGPKGLQATNVTAA"
     gene            518..736
                     /gene="infA"
                     /locus_tag="VIL39_06825"
     CDS             518..736
                     /gene="infA"
                     /locus_tag="VIL39_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5481175.1"
                     /translation="MSKEDSIEVTGTVKEKFPSGLFSVQLEHERLVLAHLAGRLRRNR
                     IRVLAGDKVTLELSPYDLTKGRITFRHR"
     gene            1006..2445
                     /locus_tag="VIL39_06830"
     CDS             1006..2445
                     /locus_tag="VIL39_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5481176.1"
                     /translation="MDKPRSMQGRAKKTASDPIEDLVTALSRKVVGQPAATKVIVPYI
                     QMFQAGLAPEGRPVGVFLLLGPTGTGKTKTVEALAEVLHGSEKNVLKVDCGEFQLEHE
                     VAKLIGAPPGYLGHRETQPVLTQQKLNAVTSEKCSLSLVLFDEIEKAAPSMTRLLLGV
                     LDKGALRLGDNSTVNFEKSLVFLTSNLGAREMMREINPEFGFQSVQGGERTGLTGKLQ
                     SIGMVSVRKRFSPEFVNRIDCIITYQPLSPEALSAILDLQMRDLQNHVNTRLGNRSFT
                     LEISLDARQFLLEKGSSPEYGARELNRTIHRFLTQPLATLVAANQVSPGAKVLVEVAG
                     NRDKLNIQSPEAEAASAPANPTVMLVDDNRDLLRFLERLMADAGWTLLTAESATEAKR
                     LMALHKPDAALLDYMLPDGNGVELGVELVQSAPAALVIVMTGTILPPEEEALCEEHNF
                     PVLRKPFLASDVMNQIRSRLASASASGRG"
BASE COUNT          540 a          804 c          807 g          474 t
ORIGIN      
        1 ccacggctgc acgctgaagc gtgcgccact cagcctgtcg gcgcctcgga aatggcaatg
       61 tcgtttcgtc acagacccta agcgaagcag gtcgctcttg cggcgttgta taattgtggt
      121 ggcattcgac tcttgatttt cgctttcgtc gtgagaaggg gttgcgtgag tctatccgcg
      181 gccccttttt tggttttgag ccgatttctc gagccgggtg cattcttgag taataaaaag
      241 agaaggaata gaagcaaatg aaagaaaaag gtacggtgaa gtggtttaac gcggccaagg
      301 gctacggctt tatccagcgg gaaaacggag aagacgtctt cgtgcatttc tccgcgattc
      361 agacggaggg ttaccgcact ctggatgaag gctcgcgagt cgaatttgag gtgacgaaag
      421 ggcccaaggg tctgcaggcg accaacgtca cggcggcctg acgatcaggc gggccgggga
      481 gcgccgctct ccggtccgcc cacccaaccg gaggtctatg agcaaagaag acagcatcga
      541 agtaaccggc accgtaaagg agaaattccc cagcgggctc ttcagcgttc agctcgagca
      601 cgagcgcctg gtcttggctc acctggcggg caggctgcgc cggaatcgca tccgcgtcct
      661 ggcgggcgac aaggtcacct tggaactgtc gccatacgat ctgaccaagg gcagaatcac
      721 cttccggcac aggtaacccg agccgtttcc catccgcatt accccagggc ggcccagtgt
      781 gcgtaagggc acacacgagc ttcccgatcg cgtgttacaa catctagtaa ggaccagcag
      841 tcacaggaga ggggaatttt ctgtgcattt caccgcggga cccttgtaag ttatggcgtc
      901 taaaagcaaa aaacatactc catgagagag cctaaccctc ccgaagagag gcggcgcagc
      961 cgggccgtgc ataccgctcc cgctatactc aagtcggaga ttagggtgga caagccaaga
     1021 agcatgcaag gccgggcgaa aaaaaccgca tcggatccca tcgaggacct cgtgaccgcg
     1081 ctctcccgga aagtggtcgg ccagccggct gccaccaagg tcatcgtgcc ctacattcag
     1141 atgttccagg cgggcctcgc gccggaggga cggcccgtgg gcgtcttcct gctgctcggt
     1201 cccaccggca ccggcaagac caagaccgtg gaggcgctcg ccgaagtact gcacgggagc
     1261 gagaagaacg tcctcaaggt cgattgcggc gaattccaac tcgagcacga ggtcgccaag
     1321 ctgatcggcg cgccgccggg ctatctcggt caccgcgaga cccagcccgt gttgacccag
     1381 cagaagctga atgcggtgac cagcgagaaa tgcagcctgt cgctggtgct cttcgacgaa
     1441 atcgagaagg ccgcgccctc catgacccgc ttgctcttgg gcgttctgga caaaggcgcg
     1501 ttgcgtttgg gcgacaactc cacggtcaac ttcgaaaaga gcctggtgtt cctcaccagc
     1561 aacctcggcg cgcgcgaaat gatgcgcgaa atcaacccgg agttcggctt ccagtcggtc
     1621 cagggcggcg agcgaaccgg cctgaccggc aaattgcaga gcatcggcat ggtctccgtc
     1681 cggaagcgct tttcgccgga gttcgtcaac cgcatcgatt gcatcatcac ctaccagccg
     1741 ctctcgccgg aggcgctttc ggcgattctc gacctgcaaa tgagggatct gcaaaaccac
     1801 gtcaacacgc gtctcggcaa tcgctcgttc accctggaga tttccctcga cgcccgccag
     1861 ttcctgttgg agaagggctc cagtccggaa tacggcgcgc gcgagttgaa ccgcacgatc
     1921 caccgttttc tcacccagcc gctggccacc ctggtcgccg ccaatcaagt aagcccgggg
     1981 gccaaggttc tggtggaagt ggccgggaac cgcgacaagc tgaacatcca gtcgccggaa
     2041 gccgaggccg cctccgcgcc ggcgaatccc acggtgatgc tggtggacga taatcgcgac
     2101 ctgctgcgtt ttctggagcg cctgatggcc gatgccggtt ggaccctgct caccgccgaa
     2161 tcggctaccg aggccaaacg cctgatggcg ctgcacaagc ccgacgccgc gctgctcgac
     2221 tacatgctgc ccgatggcaa cggcgtggaa ctcggtgtgg agcttgtcca gtccgcgccc
     2281 gcggcgctgg taatcgtgat gacggggacg attctcccgc ccgaagagga agcgctctgt
     2341 gaggagcaca atttcccggt attgcgaaag cctttcctgg cctcggacgt gatgaaccag
     2401 atccgcagcc ggctggcttc ggccagcgcc agcgggcgcg ggtagccccg tagctgtcag
     2461 ctatcagcca tcagctttat gaagccggcc ttcggccggc gttgggggct gatggctgac
     2521 ggctgatggc tgatagctat tgcgttaaaa tggcacgcat gccccgcgtg ctggtcatcc
     2581 actggaacgc ccccgaggcc gccgagcgcg cacagcggtt gcgcg
//
LOCUS       DATCGS010000335         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_374505, whole genome shotgun sequence.
ACCESSION   DATCGS010000335 DATCGS010000000
VERSION     DATCGS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374505"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VIL39_06835"
     CDS             <1..690
                     /locus_tag="VIL39_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481177.1"
                     /translation="SGNQTVVPKTEDVPREKFTPLQPARTVQPAQEAQPEIKPPQPAR
                     PVQPAQVAQPETKPKPAQPPGDLVMAKPSPTPRVTEGDANEAKPARPRTIKEALARQQ
                     DSRVPGEKMKQEGGVSRRHEMASLDTKATPFGAYDAALVEAISQRWFTLLDQREYASD
                     SRGKVVLQFRLHYDGRITDMNVSECTAGEVLGLICQKAVLDPSPFAAWPSDMRRTLGN
                     VRNIQFTFYYY"
     gene            697..1308
                     /locus_tag="VIL39_06840"
     CDS             697..1308
                     /locus_tag="VIL39_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5481178.1"
                     /translation="MEPVVYVLLFVTSVVGFTFIVERGIALRWRRVVPSQVEAAVESC
                     QAPEDVPMLRRVCQQHKSPTSRLLLIAAEHLDWPKAEAVDAIQSRARQEIARLERGLV
                     VLEIIVGIAPLLGLVGTILGMMTAFGNVGKSGQIDPAELAKGISLILRATLFGLLIAI
                     PSLIFWSYYTKKVESIAVEMESLCDEFLRRQYREGAQSPKSKV"
     gene            1345..1770
                     /locus_tag="VIL39_06845"
     CDS             1345..1770
                     /locus_tag="VIL39_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5481179.1"
                     /translation="MQFYTHKRRQAPAVIIVALIDILIVLLIFLMVTTTFKQQPALKL
                     ALPESSQAQKSGANETSPLVVSIDSKGILRLGADARPVTVERLKQELLIAAAKTSDVK
                     LAISADTAAPFGVIVKVMDAAKEAKIKMVNAFTKETGKP"
     gene            2015..2293
                     /locus_tag="VIL39_06850"
     CDS             2015..2293
                     /locus_tag="VIL39_06850"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY5481180.1"
                     /translation="MPVLAKFYGIVIRMLFDQTFGTHFHAFYGDSELVIGLNPLRVIQ
                     GEAPPWVREWALDWVRHHQNKLRSAWTIDLNVATPISRQAAGHLVFAD"
     gene            complement(2438..>2833)
                     /locus_tag="VIL39_06855"
     CDS             complement(2438..>2833)
                     /locus_tag="VIL39_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="abhydrolase domain-containing 18"
                     /protein_id="HEY5481181.1"
                     /translation="LSAAVLAAPGLDMNVFLSAAKQVVWPGLREQLLRQQPACEALNL
                     TALNLTTTRPCIPKTNILLIEAIHDLFVQRKSMEALWQAWGQPDIWRLPHSHASKSLL
                     PGLADRILRWLAPRLEDSAAKTSPNNKNH"
BASE COUNT          550 a          909 c          853 g          521 t
ORIGIN      
        1 agcggcaatc aaaccgtagt gcccaagacc gaggatgtcc cgcgcgagaa gttcaccccg
       61 ctgcaacccg cgcgcacggt ccaaccagcc caggaagccc agccagagat caagccgccg
      121 caacccgcgc gccccgtcca accggcccag gtagcccagc cggagaccaa gcccaagccg
      181 gcccagcctc cgggcgactt ggtgatggcc aagccgtccc cgacgccccg tgtgaccgag
      241 ggcgacgcca acgaagccaa acctgcccgc ccgcgcacca ttaaagaagc gctggcgcgc
      301 cagcaggaca gccgggtgcc cggggagaaa atgaaacagg agggcggggt gagccgccgt
      361 catgaaatgg cctcgctgga caccaaggcc acgccgttcg gcgcctacga tgccgcactg
      421 gtggaggcga tctcccagcg ctggttcact ctgctcgacc agcgcgagta tgcttccgac
      481 agccgcggca aggtcgtgct gcaatttcgc ttgcattacg atgggcgcat caccgatatg
      541 aacgtgtccg agtgcaccgc cggcgaagtg ctggggttga tctgccaaaa ggcggtgctc
      601 gacccgtcgc cgttcgcggc gtggccgagc gacatgcgcc gcacgctcgg caacgtccgg
      661 aacatccagt tcacttttta ctactattga ttacgtatgg aaccagttgt ctatgtcctg
      721 ctctttgtga cctcggtcgt gggctttacg tttatcgtcg aacgcggcat cgccctgcgc
      781 tggcggcggg tggtgccatc gcaagttgaa gccgcggtgg aatcctgcca ggcgcctgag
      841 gacgtgccca tgctgcggcg cgtatgccag cagcacaaat cccccaccag ccggctgctg
      901 ttgatcgccg ccgagcatct ggactggccc aaggcggagg cggtggatgc tattcaaagc
      961 cgcgcccgcc aggaaatcgc ccgcctggag cgcggcctgg tggtgctgga gatcattgtc
     1021 ggcatcgccc cgctcctcgg cctggtcggc accatcctgg gcatgatgac cgccttcggc
     1081 aacgtcggca aatccggcca gattgaccca gccgagctgg ccaagggcat ctcgctgatc
     1141 ctgcgcgcga cgctcttcgg tctgctgatc gccatcccct cgctcatctt ctggagctat
     1201 tacaccaaga aggtcgagtc aatcgcggtc gagatggagt ccctgtgcga tgagttcctc
     1261 cgccgccaat atcgggaagg agcccaaagt ccaaagtcca aagtctaagg tcacgcatca
     1321 cgcatcacgc atcaggctcg caccatgcag ttttacactc acaaacggcg gcaggcgccc
     1381 gcggtgatca ttgtcgcact gatcgacatc ctcatcgtcc tgctgatctt cctgatggtg
     1441 acgaccacct ttaagcagca gcccgccctc aagctcgccc tgcccgaatc atcccaggcc
     1501 caaaagtccg gcgcgaacga aacctcgccc ctggtcgtca gcattgattc caaaggcatc
     1561 ctgcgcctgg gcgccgatgc caggccggtg accgtcgaac gcctgaaaca ggaactcctg
     1621 atcgctgccg ccaagacttc cgacgtcaag ctcgccatca gcgccgacac cgccgcgccg
     1681 ttcggcgtga tcgtcaaggt catggacgcg gccaaagagg cgaagatcaa gatggtcaac
     1741 gccttcacca aagagaccgg caaaccctga gcactcggct cagaccggcc ctcggagtgg
     1801 ggattctatc gaacgttgcg cagtcctgtg cagaagttgc acgccgtcgc gcccggctcc
     1861 gagccgtttc ctgccgattt ggcctgaatc cgtgcctgtt gagcagtggc acccttcttg
     1921 cggaggcatt gggcgaacgc gaccgttaga tcacagacgt aaccagttgc tctgctgtca
     1981 tgtgaggcgg cgcgtaagaa aacgaaagac caatatgcca gtgcttgcca aattctacgg
     2041 gatcgtgatc cgaatgctat tcgaccagac gttcggaacg cacttccacg ccttctatgg
     2101 cgactctgag cttgtcatcg gtctgaaccc tcttcgggtg atccagggcg aggctccgcc
     2161 gtgggtgcgg gagtgggcgc tggattgggt gcggcatcac caaaataaat tgcgctctgc
     2221 ctggaccata gacctgaatg tggcaacgcc aatttcccgc caagcggcag ggcatctcgt
     2281 cttcgcggac tgaggctagc tcggcatgcg aggccaaccc ccttcgcggt aaaggcaatg
     2341 ttcgtcgcct ccgggacacc gcgtttggcc acgaacgaac cgtcggccag agtgatcatg
     2401 ctcgcgagcg gccagcctcg gcccaccggc gttcgctcta gtggtttttg ttgttgggcg
     2461 aggtcttagc ggcagagtcc tccagccgcg gcgccagcca gcgaaggatg cggtccgcca
     2521 aacccggcaa caggcttttg ctggcgtggc tgtgtggcag ccgccagatg tcgggttgtc
     2581 cccacgcttg ccagagggcc tccatggatt ttctttgcac gaagagatcg tggatggctt
     2641 cgatcagcaa tatattggtc ttggggatgc acggtcgtgt ggtggtcaag ttcaacgcgg
     2701 ttagatttaa cgcttcgcag gccggctgtt gcctcagcaa ttgttctcga aggccaggcc
     2761 agacgacttg ttttgccgcg gagagaaaga cgttcatgtc caggccgggc gcggccagga
     2821 cggcggcaga cag
//
LOCUS       DATCGS010000336         5573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_128842, whole genome shotgun sequence.
ACCESSION   DATCGS010000336 DATCGS010000000
VERSION     DATCGS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128842"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            682..1719
                     /locus_tag="VIL39_06860"
     CDS             682..1719
                     /locus_tag="VIL39_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5481182.1"
                     /translation="MSNNGNPKTLVPVTGRRDVLRAGALGLAGILAFGRAPAFAQVTP
                     KKLVFAYAIPVGPSALAFDAFAKEVNARSKGELDVQFFGATLITKELEVMNAVKSGNI
                     AIGGASGAAATVFPDMGIFLVPYLVKSYDQAYKMLNGAIGDKLDRDIQEKHKLKVLLF
                     YDYGFRHFWTTKRPIVEPKDLRGLKIRVQPAKVFSDTINGLGGNAVPMAFGEMFSAAK
                     QGVIDGGDLPIVNQLSLKIWEVSTYCSMTFHNYGPTLIVMNLEVWNGLTAAQQKLILD
                     ASREAQEKIRKATESVDNFAKAKELLGSHGMKVVQSDVEAFRKVAQEKIWPAYKQQFG
                     ALWDQVASAKV"
     gene            1758..2249
                     /locus_tag="VIL39_06865"
     CDS             1758..2249
                     /locus_tag="VIL39_06865"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5481183.1"
                     /translation="MAKLLPQIPRFVIALLLVVAIVNLLIGVFLRYVMVDITDWLDID
                     PIRFTWVEEVGEFTLAWLTLLGAAVGVRERMHFTLHVLPLTPAVRRWIDRLNHVLIAA
                     FGLVTAWYGVGLCAINTSVKSAGLEISLAILYGSSVAGGVLIAIYAISMIIAPPKAVQ
                     SDH"
     gene            2252..3541
                     /locus_tag="VIL39_06870"
     CDS             2252..3541
                     /locus_tag="VIL39_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY5481184.1"
                     /translation="MLLILSLVALLFVCLIVLSMPIVFALGIAGIVGLWAGGYSMQML
                     PSSLVASSQSWVLLAIPAFVFAGGLMERCGMAHALVDFARALVGWVKGGLGMSVLVVA
                     YFFSDICGSKMAEVSALSSTLMPALAHAGYKPEDSVSLIASGTAMGMLVPPAIFMIVI
                     AQVTNTSAVALFLAGFVPAATILFCLMVLVWFRARRYDWPVDTKPSVRFLLQSAQRAA
                     VPLVIPIVILGGFFFGVFTATEAGAVCAGYAFLVAKFYYRNVSYRQMAQLAFESATLT
                     AAVVFLLAVASIFQYLMGVSGVPNLLADLLGPLKNEPRLFLLATALITMLFGMVLEGL
                     PAAVVLIPVVFPIAEAMGINPIHFNIVQTAAVGIGLFLPPMGVGLLMALKFANVSVGR
                     HAKTYWPYLLALMVGLMLIILIPELCLTLPRRAGLIR"
     gene            complement(3755..3845)
                     /locus_tag="VIL39_06875"
     tRNA            complement(3755..3845)
                     /locus_tag="VIL39_06875"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3810..3812),aa:Sec,seq:tca)
     gene            complement(3922..4833)
                     /locus_tag="VIL39_06880"
     CDS             complement(3922..4833)
                     /locus_tag="VIL39_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5481185.1"
                     /translation="MNGTHDVVVIGAGVAGLSAALESARHGLRVCLIEELLFGGLVVN
                     VNHLLPAVAGLPASGIDLAAELMTRVSDLGVTSVFESVTGLQTGNGLSVMTAEGAHRA
                     QAVIVASGARLRKLGIPGEAEFQDRGVSHCADCDAPLYQGQDVMVVGGGDSALQEALV
                     LSEHCARVHLVHRGASFSARREFVEKLRERPNILRKMETVVEELRGEEALSRVTIRGA
                     DGTSAEISCTGFFAYVGLEPNTSFLSDQSALDLHGRVMVDAQLESAIRNVYAIGVVRA
                     GYGGQLEDAMFDARAAAIAVARRLGRV"
     gene            complement(4830..>5573)
                     /locus_tag="VIL39_06885"
     CDS             complement(4830..>5573)
                     /locus_tag="VIL39_06885"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEY5481186.1"
                     /translation="RVFNRRRDRIEELLPPDATVLGTVHRVAFSSQWLDSLQARVLEW
                     LACFHRENSSETGVNASVLQQELAAGVDAAIFAVILKRYVTEGRIVLQGSLVRLPGHD
                     STDNPRDVRLWQQVRPLLEDAGIDIPSVRELAVRVNLPLQVLRDFLHRKSVTGELIKV
                     TPERFALRPTLTALAAKARDTARSFPDGMFTAAQYRDHIGTGRTFAIEILECLDRLDI
                     THREGNGRHYTGGDLPVLPGAGNDQGSKK"
BASE COUNT         1013 a         1694 c         1689 g         1177 t
ORIGIN      
        1 cctccgggac taccaaggtg attttttctc tcatgattta attgaactcc tgcatcgttt
       61 aagttctgaa ttttactgtg cccgcgattc cgggcgagcc gggtttaaac ttgagtgatg
      121 aaacacggga gttgaacgga aacgattgac aactatgcca ggataaggac caagcgcaac
      181 cttactttcg tcaggctcaa tctacttttg cgacagcggc cagcaccaca ccatcttatt
      241 aaattttgcg atgtccgatc tcaggtgctc ggtgacttgc tccggggcca tgctcaccgc
      301 gttcggtcct tgcttggccc aaacctcttt gattttgggc aaaaccaaca caccaacgct
      361 ttggcaaggt ccgaattcag tccgttgaca atctcccgta gacttccaca ctagttcgat
      421 gatcctcgtg gtagagggcg atgtcaaata ctatgactat gctcaggcta gacgaataca
      481 cgtcctcgcg gcgcatcaat acgccgatct cgtcgggttt ggtgcagcgt tattccttgc
      541 ggctttcaga cgaattatcc gttctacacc cacttgatct gcagaagagc ctctttttgg
      601 tgtttgacaa gatgtcagcg agtctgcgat gctgcctgtg gatagtctaa agatagttaa
      661 aaagtttagg aggagagact gatgagcaac aatggaaatc cgaaaacact tgtgccagtc
      721 accgggcgcc gcgacgtgct gcgcgccggc gccttgggtc tcgcgggcat cctggccttc
      781 ggccgcgcac cggctttcgc ccaggtgacc ccgaagaagt tggtgttcgc gtacgccatc
      841 ccggtcgggc catccgccct tgctttcgac gcgttcgcca aggaggtgaa cgcgcgctcc
      901 aagggagagc tcgacgtgca gttcttcggc gccacgctca tcaccaagga actcgaggtc
      961 atgaacgcgg tgaaatccgg caatatcgcg atcggcggtg caagcggcgc cgcagccacc
     1021 gtgttcccgg atatgggtat attcctggtg ccatatctgg tgaagagcta cgaccaagcc
     1081 tacaagatgt tgaacggcgc gatcggcgac aagctggacc gggacattca ggagaagcac
     1141 aagctcaaag tactgctttt ctacgactat ggctttcgcc atttttggac caccaagcgg
     1201 ccgatcgttg agccgaagga tctgcgcggc ctgaagatcc gggtgcagcc ggccaaggtg
     1261 ttcagcgaca ccatcaacgg cctcggtggc aatgcggtgc cgatggcctt tggcgagatg
     1321 ttttcggcgg cgaagcaggg agtgatcgac ggcggcgact tgccgatcgt caaccagctc
     1381 tcattgaaga tctgggaagt gtccacctat tgctcgatga cgttccacaa ctacggtccg
     1441 acgctgatcg tcatgaacct ggaggtctgg aacgggctca ctgcagcgca gcagaagctg
     1501 atcttggacg ccagccgtga ggcacaggaa aaaatccgca aggcgaccga atcggtcgac
     1561 aacttcgcca aggccaagga gctactgggg tcgcatggga tgaaggtggt acagagcgac
     1621 gtcgaggcgt tccggaaggt ggcgcaggag aagatctggc cggcctacaa gcagcaattc
     1681 ggcgcgttgt gggaccaggt cgccagcgca aaggtgtaga ccttcgccat gacactgtcc
     1741 ttcgcgtgag ctgaccgatg gccaagctgc ttccgcagat tccccgcttc gtgatcgcgc
     1801 tgttgctggt ggtcgcgatc gtcaacttgc tgatcggcgt attcctgcgt tacgtgatgg
     1861 tcgatatcac cgattggctg gacattgacc caatccgctt cacctgggtc gaggaggtcg
     1921 gtgagttcac cttggcgtgg ctgacgctgc tcggcgccgc cgtcggggtg cgcgagcgca
     1981 tgcatttcac gctgcacgtg ctccctctga cgccggccgt gcggcgatgg atcgaccggc
     2041 tcaaccacgt cctgatcgcc gccttcggcc tcgtcaccgc gtggtacggc gtcgggctct
     2101 gcgcgatcaa cacttcggtc aagtccgcgg gcctggagat cagcctggcc atcctgtacg
     2161 gctcgtcggt ggctggcggc gtgctgatcg cgatctatgc catttcgatg atcatagcac
     2221 cacccaaggc ggttcaaagc gaccactgat catgctgctc atattatcgt tggtcgcgct
     2281 gctcttcgtc tgcctgatcg tgctgtcgat gccgatcgtc tttgcgctcg gcatcgccgg
     2341 catcgtcggt ctgtgggccg gcggctactc aatgcagatg ctgccctcca gcctggtcgc
     2401 cagttcgcag agctgggtgt tgctggcgat cccggccttc gtgttcgccg gcgggctgat
     2461 ggagcgctgc ggcatggccc acgccttggt cgatttcgcc cgtgccctgg tcggctgggt
     2521 gaagggcggg ctcggcatgt cggtactcgt agtcgcgtac ttcttctccg acatctgcgg
     2581 ctcgaagatg gccgaggtct ccgcgctgag ttcgaccctg atgccggcac tggcccatgc
     2641 cggctacaag ccggaggact ccgtctcgtt gatcgcctcc ggtaccgcca tgggcatgct
     2701 ggtgccgccg gcaatcttca tgatcgtgat cgcccaggtc accaacacgt cggcggtggc
     2761 gctgttccta gccggcttcg tgccggcggc gaccatcttg ttctgcctga tggtgctggt
     2821 gtggttccgt gcacgccgct acgactggcc ggtcgatacc aagcccagtg tgcgcttcct
     2881 gctgcagtcg gcgcagcgcg cggcggtgcc gctggtgatt ccgatagtga tccttggtgg
     2941 ctttttcttc ggtgtgttca ccgcgacgga agccggcgcc gtgtgcgccg gctacgcctt
     3001 cctggtggcg aagttctatt atcgcaatgt gagctatcgc cagatggccc agctagcgtt
     3061 tgaaagcgcg accctcacgg ccgcggtcgt gtttctgctc gccgtggcat cgatcttcca
     3121 gtacctgatg ggcgtctccg gcgtgccgaa tctcctggcc gatctgctcg gcccgctgaa
     3181 aaacgaacca cggctgttct tgctggcgac ggcgctgatc accatgctat ttggcatggt
     3241 gctggagggc ttgccggcgg cggtggtgct gatcccggtg gtgtttccga tcgcggaagc
     3301 catgggtatc aatccgatcc atttcaacat tgtgcaaacc gccgcggtgg gcatcggcct
     3361 gttcctgccg cccatgggcg tcggcctgct aatggcgctc aaattcgcca acgtgtcggt
     3421 cggccgtcat gccaagacct attggccgta tttgcttgcc ttgatggtcg gcctgatgtt
     3481 gattatcctg attccggagc tctgcctgac gctgcctcgg cgggccggct tgatccgcta
     3541 atcccgactc tagcctgttc gtcggcatgg cgatctccga cctctcgctg accgcggtgt
     3601 cgcgcgccat ctggccgacg gtattgctga tgatggcggt gctgatgctg atcacctacg
     3661 ttccggcgtt cgcgctctag ctgcccggct tggtgtaccg ctagagtact tgcgcaggtt
     3721 tacgacctgg tgaagcgccg gaggatgtga ggggcggaac ggagttggag tcgaaccaac
     3781 gcaatactgt caacagcctc gaccggattt gaagtccggc cgctccaccc gtatgattcc
     3841 cttccatgtg agcctcaaag tgcggggagc agcaagtgca agcgaatgcg gccggcgcga
     3901 cggtgagcgt aaatagacgc gttaaacgcg acctagccgc cgggcgaccg caatcgccgc
     3961 cgcgcgggcg tcgaacatcg cgtcctcgag ttgaccaccg taacccgcgc gtacgacccc
     4021 gatcgcataa acattccgaa ttgcgctttc aagttgagcg tctaccataa cccggccgtg
     4081 gagatccaga gccgattgat ccgacaggaa ggacgtgttg ggctccaggc cgacataggc
     4141 gaaaaatccg gtgcacgaga tctcggctga tgtgccatct gccccgcgaa tcgtaacccg
     4201 ggaaagcgct tcttccccgc gcagttcctc caccacggtc tccatcttgc gcaaaatatt
     4261 ggggcgttcc cgcaatttct cgacaaactc ccggcgggcg ctgaacgacg cgccgcgatg
     4321 cacgaggtgg acccgagcgc agtgctccga cagtaccagc gcttcctgca gggccgagtc
     4381 cccgccgccc acaaccatta catcctgtcc ctgatacagc ggcgcatcgc agtcggcgca
     4441 atgagagacg ccgcggtcct ggaactcggc ttcgcctgga atgccgagtt tgcgcagccg
     4501 cgcgccactc gcaacgatga cggcctgcgc tctatgtgcg ccttccgctg tcatgacgga
     4561 gaggccattc ccggtctgga gcccagtcac cgattcaaag acgcttgtga caccgagatc
     4621 tgagacgcgt gtcatcagtt ctgcggccag atcgatgcca gatgccggca aacccgccac
     4681 tgctggcagg agatggttca cgttgacgac cagaccaccg aacaagagtt cttcaatcaa
     4741 acaaacacgc aggccatgcc gggcggattc cagtgcggca ctgagtccgg ctacgcctgc
     4801 gccgatgacg acgacatcgt gggtgccgtt catttcttgc tcccttgatc gtttcccgcg
     4861 ccgggcagca ccgggaggtc gccgccggtg taatggcggc cattaccttc ccggtgcgtt
     4921 atgtccagcc tgtccaggca ctcgaggatc tcaatcgcaa aggttcgccc ggtgccgatg
     4981 tggtcgcggt attgggccgc ggtgaacatg ccgtctggga aggaacgtgc cgtgtcgcgg
     5041 gccttcgcgg ccagtgccgt cagcgtcgga cgcagggcaa agcgctccgg tgtcaccttg
     5101 atcagttcgc cggtgacgct cttgcgatgc aggaagtcgc gcagcacttg cagcggcagg
     5161 ttgacgcgga cggccaactc acgcacggaa gggatgtcta tgcccgcgtc ttccaacagg
     5221 gggcgcacct gctgccacag ccgcacgtcg cgcggattgt ccgtggagtc atgcccgggt
     5281 aggcgcacca gcgacccctg caaaacgatc cgaccttccg tgacatagcg cttcagtatc
     5341 acagcgaaga tggccgcatc gaccccggcc gccagttcct gctgcaggac ggaggcgttc
     5401 acgccggtct cgctgctgtt ttccctgtgg aagcaagcga gccattcgag cacacgcgct
     5461 tgcaggctgt cgagccactg cgacgagaac gccacgcgat ggacggtgcc gagtaccgtc
     5521 gcatccgggg gaagcaactc ctcaatacga tcccggcgac gattgaaaac tcg
//
LOCUS       DATCGS010000337         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_128952, whole genome shotgun sequence.
ACCESSION   DATCGS010000337 DATCGS010000000
VERSION     DATCGS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128952"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..369)
                     /locus_tag="VIL39_06890"
     CDS             complement(136..369)
                     /locus_tag="VIL39_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481187.1"
                     /translation="MSKRIKDEFTKLKISRQWRYQLRMRRDKRCIICGTPITRGLMCL
                     EHMIQGRERQRKRLGLKRRHRNALTYRLQRKEA"
     gene            complement(427..1305)
                     /locus_tag="VIL39_06895"
     CDS             complement(427..1305)
                     /locus_tag="VIL39_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5481188.1"
                     /translation="MQFESLKVFCDLAETESFTKTAQINSVSQSAVSQTISALERNFK
                     SLLIERSKKNFRLTTEGEVLYHYSKEILQTYDAIHSRMQEIKHVISGDIRVATVYSIG
                     LYDLPPYVKHFMQSHPDVNIHVEYRRSNQVYEGVLGNIVDLGLVAYPAPDPKLQIVPL
                     RKDRLVLACHPDHALAKLKSVRLKALNGQKVISFEPDMPTRKALDKMLKGQGVTVEHV
                     MEFDNIETVKRAVELDCGVAIVPETNIRQEVTKQTLASVPLEGNHFRPLAAIHRKNKV
                     LSPAIKHFIALLKKPI"
     gene            1473..2192
                     /locus_tag="VIL39_06900"
     CDS             1473..2192
                     /locus_tag="VIL39_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5481189.1"
                     /translation="MGSETSQVEAVLAVREPGAYTPGMIILTNTGGVAMTNCFLIADE
                     TARQAVMFDAPDHTTGPLLKEAATRGWDLIGLWLTHGHFDHFADHAVVRQKFPAAKIL
                     LHALDEPKAQHPEVQTRLFGLPFVIPPLKADAYVTDNQKLKIGSLEVVVIHTPGHSPG
                     HVVYHFPKEKVLVGGDLIIGGSIGRTDLPDSDHQAMEASIRKVMGLPAATRLLGGHGD
                     ATTLEEERQTNPFMREILAGS"
     gene            complement(2240..3685)
                     /locus_tag="VIL39_06905"
     CDS             complement(2240..3685)
                     /locus_tag="VIL39_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5481190.1"
                     /translation="MSKLLLIDDEADVQYSFQRIFDSPDLELTTASSGEEGLRIIPKL
                     KPDLVIMDVRMGGLTGLETLRRIRQANPKLLVILMTAYGTTQTAIEAMKLGAYDFLLK
                     PFDVTKLKEIVFNALKAASDMRQVVSYQPLLEKEDYELGIIGRSEPMQQVFKLIGQLA
                     ASDATALISGESGTGKELVARAIYHHSHRSQQPFLAINCAAIPEQLLESELFGHERGA
                     FTGATLQRLGKFEQCNHGTLFLDEIGDMTPATQTKILRVLQSGTFERVGGNQPISVDV
                     RIIAATNKPLEQAVAARQFREDLFYRLNVVRIHLPPLRERREDIRLLVNYFLKAFVKD
                     QQHPPKSIAPGVIRALEKYHWPGNVRELENVLRRALVVAKGDAILLSDLPPEILGQGP
                     AAGAAVTVSLPAGDGSRASIADMARQLFQWARRDPKLKLIPAVERELIIHALKETDGN
                     QLQAAKLLGITRATLRKRVEKFGIQRGLSIT"
     gene            complement(3682..>3864)
                     /locus_tag="VIL39_06910"
     CDS             complement(3682..>3864)
                     /locus_tag="VIL39_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481191.1"
                     /translation="ADAPDFIISLPLSNLAAPEDGRTPPRPSPAVTDPLPLGLKIRCR
                     NGGNELPSDLIAGKTL"
BASE COUNT          705 a         1114 c         1217 g          828 t
ORIGIN      
        1 ggctattatc ggatgtccct ccgggacaaa gacttgcccc ggtctggcgt attttgtggg
       61 agcaaatcct agcggcattg gacttcgggg tctgcgctgc aaggttgccg gcttcgcggc
      121 cggcgaaagg ccgggctagg cttccttccg ctggagccga taggtcaacg cgttgcggtg
      181 gcggcgcttg aggcccagtc gcttccgctg ccgctcccgg ccctggatca tgtgctccaa
      241 acacattagc ccccgggtga tcggtgtgcc gcagatgatg caccgcttgt cccgccgcat
      301 ccgcagttga taccgccact ggcgggagat cttcagcttc gtgaactcat ccttgatgcg
      361 tttgctcatt tctcaatggg ataacgtttg ttggaatact cacccgcgcc gaccgcagcg
      421 gacctttcaa attggctttt tcagcagggc gatgaagtgc ttaatggccg gggacagcac
      481 tttgttcttc cggtggatcg cagccagcgg ccggaaatga ttgccttcga gcgggaccga
      541 cgccagcgtt tgtttagtca cctcctgccg gatgttagtt tctggcacga tcgccacgcc
      601 gcagtcgagt tcgacggccc gcttcaccgt ctcgatatta tcgaactcca tgacatgctc
      661 gacggtcacg ccctggcctt tgagcatttt gtccagcgct ttgcgggtgg gcatgtccgg
      721 ctcgaagctg atgaccttct gcccgttcag cgccttgagc ctgacggact tgagtttcgc
      781 cagggcatgg tccggatggc aggcgagcac cagccggtct tttcgcagcg ggacaatctg
      841 cagtttcggg tcgggagccg ggtaggcaac caggcccaga tccacgatgt ttcccaacac
      901 cccttcatac acctggttgc tgcggcggta ttcgacgtgg atattcacgt ccggatggct
      961 ctgcatgaag tgtttgacgt aagggggcaa gtcgtacagc ccaatgctgt aaacggtcgc
     1021 cacccgaatg tcgccggaaa tgacgtgctt gatctcctgc atccggctat ggatggcatc
     1081 gtaggtctgg aggatctctt tgctgtagtg gtaaaggact tcgccttccg tggtgagccg
     1141 gaagttcttc ttgctgcgct cgatgaggag ggatttaaag ttccgctcca aagcgctgat
     1201 cgtctggctg accgccgatt ggctgacgct gttgatttgc gcggtcttgg taaagctctc
     1261 ggtctcagcg agatcgcaga atactttgag gctttcaaat tgcataatgc gattgaatag
     1321 aaaaactgcc tgacagtcaa tgagcgcttg aggcctcccc gcatcagccg gagcgctctg
     1381 tcccctaatc cgtccaaact catttattca cgctggtggg gcatctgcgg ggatttgggc
     1441 ctcaaccacc aaggatttgc aatcaaattc atgtgggctc agagacttcg caagtcgaag
     1501 ccgtgctggc cgtccgtgaa ccgggcgcat acactcccgg catgatcata ctgacgaaca
     1561 ccggcggggt cgcgatgacc aattgcttct tgattgcgga cgaaacagcc aggcaggcgg
     1621 tgatgtttga cgcgcccgac cataccaccg gcccgctgct taaggaagca gccacccgag
     1681 gctgggacct cattgggttg tggctcaccc acggccactt cgaccacttt gccgatcatg
     1741 cggtcgtgcg gcagaagttt cccgccgcca agatcctgct ccacgcgctg gatgagccca
     1801 aggcgcaaca tccggaggtg cagacgcggc tgtttggact gcctttcgtc attccgccgc
     1861 tgaaggcgga cgcgtatgtg accgacaatc agaagctcaa gatcgggtca ctggaggtcg
     1921 tcgtgattca taccccgggc cattcgccgg ggcacgtggt gtatcatttc cccaaagaga
     1981 aggtgctggt ggggggcgac ctgatcattg gcggttccat tggccgaacc gatctgccgg
     2041 actcggacca tcaggcaatg gaagcctcga tccgcaaggt gatgggcttg cccgcggcga
     2101 cgcgcctgct gggcgggcac ggggatgcca cgaccctgga agaggaacgg cagacaaatc
     2161 cgttcatgcg ggagatactg gctgggagtt gagagcgtag cggcggacag aagactaccc
     2221 gcccggctcg gcagtcagtt caggtaatgc tcagcccgcg ctgaatcccg aacttctcga
     2281 cccgcttgcg cagcgtggcg cgcgtgatgc cgagcagctt ggcggcctgg agttgattgc
     2341 cgtcggtctc ctttaatgcg tggatgatca actctcgctc gaccgctggg atgagcttca
     2401 gttttgggtc gcgccgggcc cactggaaca attgccgagc catgtcggcg atgctcgctc
     2461 tgctgccatc gcccgctggc agggagaccg tgacggcagc tccggcggct gggccttgcc
     2521 cgaggatctc cggcggcaaa tcgctcagca gaatcgcgtc ccccttggcc acaaccagcg
     2581 cgcggcggag aacgttttcc agttcgcgca cgttgccggg ccagtggtat ttctccagcg
     2641 cccggatcac gccgggggcg atggatttgg gcgggtgctg ctggtctttg acaaaagcct
     2701 tgaggaagta attgaccagc agccggatgt cctcgcgccg ctcgcgcaat ggcggaaggt
     2761 ggatccgcac cacgttcagc cggtaaaaca gatcctcgcg gaactggcgc gcggccaccg
     2821 cctgctccag cggcttattg gtcgcggcga tgatgcgcac gtccacggaa atcggctggt
     2881 tgccgcccac gcgttcgaag gtgccggact gcaacacccg caggattttg gtctgggtcg
     2941 cgggcgtcat gtcgccgatt tcatccagga agagcgtgcc gtggttgcat tgctcgaact
     3001 tgccgaggcg ctgcagggtc gcgccggtga aggcgccacg ctcgtggccg aacaattcgc
     3061 tttcaagcaa ttgctccggg atggcggcgc aattgatggc gagaaacggt tgttggctgc
     3121 ggtggctgtg atgataaatg gcgcgcgcga ccagttcctt gcccgtgccg ctttcgccgc
     3181 tgatcagcgc ggtggcgtct gaggcggcga gctggccgat cagcttgaag acctgctgca
     3241 tcggctcgct gcgcccgatg atgcccagct cgtaatcttc cttttccagc agcggctggt
     3301 aggacacaac ctgccgcatg tcgctcgcgg ccttcagggc attgaagacg atttctttga
     3361 gcttggtcac gtcgaacggt ttgagcagga aatcgtaggc gcccagcttc atcgcctcaa
     3421 tggcggtttg cgtcgtgcca taggcggtca tcaggatgac gagcaacttg gggttggcct
     3481 ggcggatgcg ccgcagggtt tccaggcccg tgaggccgcc catgcgcacg tccatgatga
     3541 cgaggtcggg cttgagcttg gggatgatcc gcaaaccttc ctcgccgctg gacgcggtgg
     3601 tcagctcgag gtccggcgaa tcaaagatcc gctggaacga gtattgcaca tcggcttcgt
     3661 cgtcaattag cagcagtttg ctcataaggt tttcccggca atcaggtcgg aaggtaattc
     3721 attaccgcca ttgcgacagc gaattttgag gcccaatggt aaagggtcag tgacagcggg
     3781 agaaggtcgt ggcggagtgc gcccgtcctc gggcgcagca aggttggaaa gcggcagaga
     3841 tataatgaaa tccggcgcgt cagc
//
LOCUS       DATCGS010000338         5609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_573775, whole genome shotgun sequence.
ACCESSION   DATCGS010000338 DATCGS010000000
VERSION     DATCGS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573775"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..417
                     /locus_tag="VIL39_06915"
     CDS             85..417
                     /locus_tag="VIL39_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5481192.1"
                     /translation="MKIPNRRRTLASNNRAHAEIDGYLKRKLQDQVFRAAYDAEDKHI
                     DLVLQIIKLRQQRGMTQADLAKAIGTRQANVSRLERFDSNLTVGTLEKVAQALGVNLQ
                     IDLVPKAA"
     gene            418..1242
                     /locus_tag="VIL39_06920"
     CDS             418..1242
                     /locus_tag="VIL39_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5481193.1"
                     /translation="MPKETWIIAGALTVALLLGLLGLSSWEKKREVEKVRAELTEAQA
                     AAAKAQAEAADLKNKLADSEARNEELHKEQAIVVHTHQSLEDEMRNALESKDVTISQL
                     QGKLTVNILDRVMFDSGEAELKPAGAAVLRKVAAVLAQHPNIKVHVIGHTDNVPIRAA
                     ARNRFPSNWELSTTRATAAVRFLTENAGVDPRRLGAVGYGEFRPVAENTTPEGRARNR
                     RIAITILSEEMAGADTAPTAAPNSPALRGSPTASTNALADPSAGVQPSDATVPAAR"
     gene            complement(1259..2527)
                     /locus_tag="VIL39_06925"
     CDS             complement(1259..2527)
                     /locus_tag="VIL39_06925"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5481194.1"
                     /translation="MANTILVGAQWGDEGKGKIIDVLTEQADLVVRTQGGNNAGHTVH
                     IKGQKYILHLVPSGILRKNKVCVIGNGVVVDPVGLVEEIEGLRKLGIRIGGNLFLSET
                     AHVVFPYHRELDAQRESFKGKNKIGTTKRGIGPAYGDKAARTGLRVLDLINPERFKEQ
                     LALKIKENNEVLKAFGAQPLSFKKVHAEYRAAGDYLKPFVANTVVLLHQAMRRGADIL
                     FEGAQGTFLDIDHGTYPFVTSSNTTAGGACTGSGVPPHRMDRVVGVMKAYTTRVGEGP
                     LPTENAEIADMLHAMGREFGATTGRARRCGWFDSIATRHACMVNGIDDLAVTNVDGLD
                     SVETIKVCIGYRDGGRRYDYVPNDIEALTRCTPIYAEFPGWRTPTHNARKWKDLPAKA
                     RSYLKALAELSDAKLFIASVGPGREQTIFV"
     gene            complement(2826..>5609)
                     /locus_tag="VIL39_06930"
     CDS             complement(2826..>5609)
                     /locus_tag="VIL39_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5481195.1"
                     /translation="AQHTDSPGSLCNFIGEQGMGVWLLTMTDNVLTHTGLVANLSIRL
                     DPQNVANGAQRDVQTNAFSFDFIDVPIGATNLTVCLYNDSDMPLPVELYLRRGDLPSQ
                     TTFDQRVPIDPPSGCLSINLSTLPPLNPGRYYLGVFNSNGIPQTVRLDTGVAFGLAGV
                     VPVTFASAGPIPLLDDAVTNASLFVSNHQVIASLEVGLLVNHPRVSDMVFTLISPSGT
                     RVPLFENRGGATTNGLGGMVVRTNMFPTKRSGDYNANTNILPLAYPQGTLFLDYEFYL
                     APDTMHVYYDSVKIYDSGPVSYAHHVSIPFGPGVSTNIVIIMNEGDNEDTNTLWEYTA
                     TVACPSPAYVVFTENTNLAPLPIKFATAPFLPSSTNLDLYCLPEKSLNALTGENAYGT
                     WQLEMWDTRVGAAYPAPELASWQLRFVFQNTVPVPVVLTHGIAQSNSIPPGQVAPFSI
                     DVPAWATRATNILVYASAPVNLLFSQTLPPTGTNAGDITLLSASSGGFFALNTNGMPA
                     LIPGASYYLGLQNPGTASVTATVQVDFDVTPLANGVPFNATQAGNTLPRYFSYDVSSN
                     GTAVSFQLLNLNGNLDLVARKASFPTLADFDCASLNPGTNDEGIVLFTDSTPVALAPG
                     RWYLGVFNADPTDAGYTIVITEYTNAFPDIITLTSGVPYPNSNYGAGDAADYYHYVVT
                     TNALRAQFEIDGPTAEVTLVARKGLPLPTLTNYACLSANAGPNEQLITLFDFSSPVAL
                     TPGDWFISVINVSGGPVDYTIMATEFPVYGTNVVITSCQTLTNSLCLTWTSEPGIHYY
                     VQGKTNVTDTNWVAVSSTLVATDVLTTWCVALPSPCQFFRVTEGLVVTPYVPPVRITS
                     ITRDTNGVLLQWLAPTNSQFQAQWTPSLAPPAWTPFTNILTFTNGAFSFLDDGSQSGG
                     LAGSRCYRLQQLP"
BASE COUNT         1144 a         1599 c         1834 g         1032 t
ORIGIN      
        1 tgatcttcac gcatggctat atcaagaaga cggacaagac ttcgcccggt gagattagtc
       61 gggccaaacg attgcgagat tactatgaaa attccgaacc gccgccgcac tttggcatcg
      121 aataaccgtg cgcacgccga aattgacggc tacctgaagc gcaagctgca ggaccaggtc
      181 ttccgcgctg cctacgatgc tgaggacaag cacatcgatc tggtgctcca gatcatcaaa
      241 ctacgccagc aacgcggcat gacacaggca gacttggcca aagctatcgg cacgcgccag
      301 gccaacgtca gccggcttga gcgattcgac agcaacctca ccgtaggcac tctggagaag
      361 gtcgcgcagg cattgggtgt aaacctccag attgatctgg taccaaaggc agcctaaatg
      421 cccaaagaga cttggatcat cgcgggcgcg ctaacggtgg ccctgctgtt ggggctgctg
      481 ggcctgagct cctgggagaa gaagcgcgag gtggagaagg tgcgggccga gctcacggaa
      541 gcgcaagccg ctgccgccaa agcgcaggcc gaagccgccg acctcaagaa caaactggcc
      601 gacagcgagg ctcgtaacga agagctccat aaagagcaag ctatcgtcgt ccacacgcac
      661 cagagcctgg aagacgagat gcgcaacgcc ctcgagtcaa aggatgtcac catctcccag
      721 ttgcaaggca agctgacggt caacatcctg gaccgcgtca tgttcgactc cggtgaggcc
      781 gagctgaaac cggcgggcgc ggcggtattg cgcaaggtgg cggccgtgct ggcccaacat
      841 cccaacatca aagtccatgt catcggccac accgataacg tccccatccg agccgccgcc
      901 cggaatcgtt tccccagcaa ctgggaactc tcgaccaccc gcgccaccgc cgccgtccgg
      961 ttcctgaccg agaatgccgg cgtggacccc cgccgcctgg gcgcggtagg ttacggtgag
     1021 ttccgccccg tcgctgaaaa cactactccc gaaggccgtg cccgcaaccg ccgcatcgcc
     1081 atcaccatcc tctccgaaga gatggctggc gccgacaccg ctccgacagc cgcgcccaat
     1141 tcccctgccc tgcgaggttc cccgaccgca agcaccaacg ctttagctga cccctcggct
     1201 ggggttcagc cctccgacgc caccgttcct gcggcgcgat aggtctcctg ccgaggtgtc
     1261 agacgaaaat cgtctgctcc cgccccgggc cgaccgaagc aatgaacagc ttggcgtcgc
     1321 tcagctcagc cagcgcttta agataggaac gcgccttggc cggcaggtcc ttccacttgc
     1381 gggcgttgtg ggtcggcgtc cgccagccgg ggaactcggc gtaaatcggc gtgcagcggg
     1441 tcagggcctc gatgtcgttc ggcacataat catagcgccg gccgccgtcg cggtagccaa
     1501 tgcagacttt aatggtctcg actgaatcca aaccgtccac gttcgtcacg gccagatcat
     1561 caatgccgtt caccatgcag gcgtgccgcg tggcgatgga gtcgaaccaa ccacaacgac
     1621 gcgcccggcc agtcgtcgcg ccaaactcgc ggcccatggc gtggagcatg tcggcgatct
     1681 cggcgttctc ggtcgggagc gggccctcgc ccacgcgtgt ggtgtaggct ttcatcacgc
     1741 ctacgacacg gtccatgcga tgcggcggga cgcccgaccc ggtgcaggcg ccgcccgcgg
     1801 tggtattcga ggaagtcacg aaaggatagg tgccgtggtc tatgtcgagg aacgtgccct
     1861 gcgcgccctc gaacaggatg tcagcgcccc gtcgcatcgc ctggtgcagc aaaaccacgg
     1921 tgttcgcgac gaaaggcttc aggtagtcgc cggcggcgcg atattccgcg tggaccttct
     1981 tgaacgaaag gggttgggcg ccgaaagcct tcagcacttc gttgttctcc ttgatcttca
     2041 atgcgagttg ttccttgaac cgctccgggt tgatcaggtc gagcacgcgc aagcccgtgc
     2101 gcgccgcctt gtcgccgtaa gccgggccga tgccgcgctt ggtggtgccg atcttgttct
     2161 tgcctttgaa actctcgcgc tgggcatcga gttcgcgatg atacggaaag accacgtgcg
     2221 ccgtctcgct gaggaagagg ttgccgccga tccggatgcc gagcttgcgc agcccctcga
     2281 tctcctcgac gaggccgacg ggatcaacca ccaccccgtt gccgatgacg cagaccttgt
     2341 tcttgcgcag gatgccggag gggaccaggt gcaggatgta tttctgtcct ttgatgtgga
     2401 ccgtgtggcc ggcgttgttg ccgccttgcg tgcgcaccac caggtccgcc tgctcggtca
     2461 gcacgtctat gatcttgcct ttgccttcat cgccccattg ggcgcccact agaatggtat
     2521 tcgccataag ttcaataaaa atccccgtac ctgcattcag ggacaaaagc gctcgactct
     2581 tgcgcgagga aactaaggag attgcttccc cctgtcaatg atggatgatg acatcgtgct
     2641 ttctcggaag cgtaccgaca ggcgcgcgcc aatttgaggc tcgcatgagg atcgcctggg
     2701 gatggcttgc ataagccttc gcaatactga gaatggcggt ttgcaccctc ttgccaaccc
     2761 ggcaccgctc tggtctataa gccggcgctc gatgacgact ccttgatgca gctccacgga
     2821 tcacgctacg gcaactgctg tagccggtag cagcgggagc cagcgaggcc gccggattgg
     2881 gagccgtcgt cgaggaatga gaacgcgccg ttggtgaagg tgaggatatt ggtaaagggg
     2941 gtccatgctg ggggcgcaag cgacggcgtc cactgcgcct ggaactggct gttggtcggc
     3001 gccagccatt gcaggaggac gccgttcgtg tcgcgggtga tgctggtgat gcggacgggc
     3061 ggaacgtagg gggtaaccac gagcccttca gtgacacgga agaactggca gggcgacggc
     3121 agggcgacac accaggtggt cagcacatcg gtggcgacaa gggtcgagga gactgcaacc
     3181 cagttggtgt cagtcacgtt ggtcttgccc tgcacgtaat aatggatgcc gggctcggag
     3241 gtccaggtga gacagaggct gttggtgaga gtctggcagc tcgtgatgac aacattggtc
     3301 ccgtaaaccg ggaactcggt ggccataatg gtgtaatcca ccggcccgcc ggagacgttt
     3361 atgacggaga tgaaccagtc gcccggcgtc agggcgaccg gggaggaaaa gtcaaagagc
     3421 gtaatgagtt gttcattcgg cccggcgttg gcgctcaggc aggcgtagtt ggtcagggtg
     3481 ggcagcggca gccctttgcg cgccaccagg gtcacctcgg cggtcgggcc gtcaatctca
     3541 aattgcgcgc gcagggcgtt agtggtgacg acgtagtgat agtagtcggc ggcatcgcct
     3601 gcgccgtagt tgctattcgg gtaagggacg ccgctggtca gcgtgatgat atccggaaag
     3661 gcgttggtgt attcggtgat cacgatcgtg tagccagcgt cggtcgggtc ggcattgaag
     3721 acgccgagat accagcgacc gggcgccaga gccacggggg tcgaatcagt gaagaggacg
     3781 ataccctcgt cgttggtgcc agggttgaga ctcgcgcagt caaagtccgc aagggtcggg
     3841 aacgacgcct ttcgggcgac gaggtcgagg ttgccgttga ggttgagcag ttggaaggaa
     3901 acagcggtgc cgttcgacga cacgtcataa gagaagtacc gaggcagggt gttgccggcc
     3961 tgagtggcat tgaatggaac gccgttggcc aaaggagtga cgtcgaaatc cacctgcacg
     4021 gtggcggtta cactggcagt ccccgggttc tggagcccga gatagtaact ggcgccggga
     4081 atcaaggcgg gcattccgtt ggtgtttagg gcaaagaagc cgccgctgga ggctgacagc
     4141 agtgtgatat cgccggcgtt ggtgcccgtg ggcggaagag tctggctaaa gagcaggttc
     4201 acgggcgcgg aggcataaac caggatgttc gtggcgcggg tcgcccaggc gggcacatct
     4261 attgaaaagg gggcgacctg accaggcggg atgctattgc tctgggcgat gccgtgagtc
     4321 aaaaccacgg gcacggggac ggtgttctgg aaaacgaagc gcaactgcca actggccagt
     4381 tctggcgcag gatacgcagc ccccacgcga gtgtcccaca tttcgagttg ccaggtgcca
     4441 taggcattct cgccggtgag ggcgttaagc gacttttcgg gcaggcagta cagatccagg
     4501 ttggtgctgg agggcaggaa cggcgcggtg gcaaacttga tgggaagcgg cgccaagttg
     4561 gtattctccg tgaacaccac ataggccgga ctcgggcagg cgaccgtagc ggtgtactcc
     4621 cagagcgtgt tggtatcctc gttgtctcct tcgttcatga tgatcacgat attcgtggaa
     4681 acgcctgggc cgaaggggat ggagacatga tgggcgtaac tgaccggccc ggagtcatag
     4741 atcttcacgc tgtcgtaata gacatgcatg gtgtcagggg ccaggtagaa ctcgtagtca
     4801 aggaacagcg tgccctgggg ataggccagt gggaggatat tcgtattggc gttgtaatcg
     4861 cctgagcgct tggtggggaa catgttggtt cggaccacca ttccgcccag gccgttggtc
     4921 gtcgcgcctc cgcgattctc aaacaacggc acgcgtgtgc cgctggggct gatgagagta
     4981 aacaccatgt ccgagacgcg cggatgattc acaagcaatc caacctcgag agaggcgatg
     5041 acctggtggt tggacacaaa gaggctggcg ttggtgactg catcgtcgag gagggggatt
     5101 ggcccggcgg aggcaaaggt gacggggacg actccagcga gaccgaaagc cactcctgtg
     5161 tccagccgga cggtctgcgg gatgccattc gaattgaaga ccccgaggta gtaacggccc
     5221 ggattaagcg gcggcagagt ggacaggttt atagacaaac atcccgaggg gggatcgatc
     5281 ggcacccttt ggtcgaacgt cgtttggctg gggagatcgc cgcggcggag gtaaagctcc
     5341 acgggcaggg gcatatcgga gtcgttgtaa aggcagacag tgaggttcgt cgcgccaatg
     5401 ggaacatcaa tgaaatcgaa ggagaacgcg ttagtttgca cgtcacgctg agcgccgttc
     5461 gcaacgttct gcgggtccag gcggatagat agattcgcga ccaggccggt atgcgtgagg
     5521 acgttgtccg tcatcgtcag cagccagacg cccattccct gttcgccaat gaagttgcac
     5581 aagctccccg ggctgtcggt atgttgcgc
//
LOCUS       DATCGS010000339         3673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_722599, whole genome shotgun sequence.
ACCESSION   DATCGS010000339 DATCGS010000000
VERSION     DATCGS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722599"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VIL39_06935"
     CDS             <1..213
                     /locus_tag="VIL39_06935"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5481196.1"
                     /translation="RTFGTLCLSVVCILAYGGMIKGILPTMAGISWEGHLAGLMAGVA
                     LGSFMSTLKKEPTAPANPGTGNVPSH"
     gene            316..3339
                     /locus_tag="VIL39_06940"
     CDS             316..3339
                     /locus_tag="VIL39_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481197.1"
                     /translation="MTAEPSPKLERQFVQNSLPCVVGAAALAVYLATLNHWVTLSSLP
                     LVARVNGWDWQPMLSQPLLCLLTFPFRWLPASWVPLALNVFAAVCASLTLATLARSVA
                     LLPHDRMEQQRLLVQNEHALLSLPNAWVPVVLAAIALGLQLTFWEHAIAASGEMLDLL
                     VFAYIIRCLLEHRVDEQPSWLDRAALLFGCAMANNWGMVGFLPLFLVALLRTKRLSFF
                     SLRTIRRIDRSGWERVAPALTTDLRFFLRMALLGLAGSSLFLLLPLAQAFSPGSTLSF
                     WQALHTVATSYWDTLHLLARVFLRSHRDVALLLAAASLLPILLLSIRWGAFVGGESHA
                     RFDLASFILHVSHAFLLLICLSSVFDPPFGPRQVARQTGLLLPFLPLYYLTALSIGYY
                     SGFFLLMFGAAAFQRTGRRNTVRRFLCRVVPILVYVLLCLVLAGLPLLNLPAIRATNA
                     PHLDQYARLAAGSLPPEGAVVLSDDPAHLAFLQAELAREGKAGRYLPVETRNLPLAPY
                     RAWLSRKNPGRWPDPGIEAEPAVAGRMASQTNAPLDGPGLVRLMFRVAQSNRVCCLQP
                     GVGFLLEQFYLQPHGLLHEMKSYPGDFLSDPPLTDAGLAENQAFWKSALETGVRPVLR
                     LVSQPELPRPAFEERLMKLGHLQTPPPAQARVLARWYSGALNRWGVTLQRNGRWSEAT
                     PCFALALELNSDNLPAFVNLQCNSNLLAHQKMTVVRTQVFPEQLGRYRSLNQVLTENG
                     PFDEPSYCYRLGMGFAEGKMRRQAGQQFDRVKALVPSDFSVRLTLGNLFNTCSRPELA
                     LGIVAEMQADPELRPLGLTNEVEVALLEARAYLTATNQATAQGIIYALLATHPGDSAL
                     LARAVTTFADYQCYSDAIRLTDHQLQLTPDDPAALANKGILCVLTGDSSNAIPTLTRS
                     LSLTNTYVARLNRAIAYLRTGRLDAAEADYQALLQQFPTAYRVSAMLGEVALQRKDTN
                     TAIRYYEQYLSKLEPDTEEARFVAVRLKSLQQGGP"
     gene            complement(3311..>3673)
                     /locus_tag="VIL39_06945"
     CDS             complement(3311..>3673)
                     /locus_tag="VIL39_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481198.1"
                     /translation="VDNVLQMCPSNAVRDGHSVLLVGYRDDAAQPGGGVFIFRNTSRS
                     GQDGWMPYAYAREYMNDAVWVDFERRTNAPTAPAAVSPLRRDPLGALTTLPAGRNRPI
                     SSNEQPKAELKARLAGAT"
BASE COUNT          604 a         1217 c         1068 g          784 t
ORIGIN      
        1 cggacgttcg gaaccctttg tttatcggtg gtgtgcatcc tggcatatgg gggaatgatc
       61 aaaggcattc tgcctaccat ggccggcatt tcgtgggaag ggcacctcgc cgggctgatg
      121 gcaggcgtgg cgctgggctc attcatgtca acactgaaga aggagcccac cgctccggct
      181 aacccaggca ccggaaacgt tccgagccat tgagagtcca tcgcatttgc tttcgcggat
      241 tgacttctca attccgtcgc cgcagcctcc gcccttgaag ttttgaagtt gtccaaagaa
      301 gccatttgct gacgcatgac cgccgaaccc tcgcccaaac tcgaacgcca attcgtgcag
      361 aacagcctgc cttgcgtggt cggggcggca gccttggcgg tttacctggc cacgcttaac
      421 cactgggtga ccctcagcag cctccctttg gtggcccggg tgaacggctg ggactggcaa
      481 cccatgctgt cccagccgct gttgtgcctg ctcacgttcc cgttccgctg gttgccggcg
      541 agttgggtgc cgctggcgct caacgtcttt gcggccgtct gcgcgtcatt gacgctggcg
      601 acgctggcgc gctcggtagc gctgttgccg catgatcgca tggaacagca acggcttttg
      661 gtccagaatg aacacgccct gctgtctctg cctaatgcct gggtgccggt cgtcctggcc
      721 gccatcgctc ttggactgca attgaccttt tgggaacatg ccattgccgc ttccggggag
      781 atgctggatt tgctggtgtt tgcttacatc atccggtgcc tgctcgaaca ccgcgttgac
      841 gaacagccat catggctgga ccgggccgcg ctcctttttg ggtgcgccat ggccaacaat
      901 tgggggatgg tcggattcct gccactcttt ctcgtggcgc tgctccggac caagcggctg
      961 agcttcttca gtctccgcac gatccggcgc atagaccgct ccgggtggga gagggtcgcc
     1021 cccgcgctga ccacagatct tcgcttcttc ctgcgcatgg cgctgctcgg gctggccggc
     1081 tcgtctctct ttcttctgct gccgctcgcg caagcgttct cgccgggttc aacccttagc
     1141 ttctggcagg ctctgcacac agtcgcgact tcctactggg acaccctcca tcttctcgcc
     1201 cgcgtgttct tgcgcagcca ccgcgacgtc gcgctgctgc tggccgccgc ctccctgctg
     1261 ccgatcctcc tgctgagcat ccgctggggc gcctttgtcg gcggagaaag ccacgcccgg
     1321 ttcgacctgg cctctttcat tttgcacgtc tcccatgcct tcctgttgtt gatctgcctc
     1381 tcgtccgtct tcgatccgcc gttcggcccg cgtcaagtcg ctcgtcagac gggtctcttg
     1441 ttgccctttc tgccgctcta ttacctcacc gctctgagca tcggttacta cagcggcttc
     1501 ttcctcttaa tgttcggcgc cgctgctttc caacgcaccg gccggcgcaa caccgttcga
     1561 cgcttcctgt gccgggtggt gcccatactc gtttacgttc ttctctgcct cgtacttgct
     1621 ggcttgcccc tgttgaatct ccccgccatc cgcgcaacca acgcaccgca cctggaccag
     1681 tatgcccggc tagccgcggg atcgctgccg ccggaaggcg cagtcgtgct gagtgatgat
     1741 ccggcccact tggcgttcct ccaggccgag ctagctcgtg aaggcaaagc gggacgctac
     1801 ctgcccgtgg aaacgcgcaa cctccctctg gcgccatatc gcgcctggct gagcaggaag
     1861 aaccccgggc gatggccaga cccgggaatc gaggcggaac cggccgttgc cgggcgcatg
     1921 gcgtcccaaa ccaacgcgcc cctggatggc ccgggtctcg ttcgattgat gttccgcgtc
     1981 gcgcagagca accgcgtctg ctgcttgcag ccaggcgttg gtttcttgct cgagcaattc
     2041 tatttgcagc cgcacggtct gctccatgaa atgaagtcct atcctgggga tttcttaagc
     2101 gacccaccgc tgactgacgc ggggctggcg gaaaaccaag ccttctggaa gtccgcgctc
     2161 gaaaccggcg taagaccggt cttgcgcctc gtatcccaac cagaactccc gcgccccgct
     2221 tttgaagaga ggctgatgaa actgggccat ctccaaacgc cccctccggc tcaagcccga
     2281 gtgctggcga ggtggtactc cggcgcgctg aatcgctggg gcgttacgct ccaacgaaac
     2341 ggccggtgga gcgaggccac cccctgcttc gcgctggccc tggaattgaa ctccgataac
     2401 ctcccggcct tcgtcaactt gcaatgcaac agcaatctgc tggcgcatca gaaaatgacg
     2461 gtggtccgga cccaggtctt tccggagcag ctcggcaggt accgtagttt gaatcaagtt
     2521 ctgactgaga acggcccgtt cgacgaaccg agctactgct accggttggg aatgggtttt
     2581 gccgagggta agatgcgccg gcaggccggc cagcaatttg accgcgttaa ggccctggtg
     2641 ccgtccgact tctccgtgcg attgacgctc ggcaatttgt ttaacacctg ctcgaggccc
     2701 gagctggccc tcgggatcgt ggccgaaatg caagccgacc ctgagttgcg gcctctcgga
     2761 ctgacgaacg aggtggaagt tgcgctcttg gaagccaggg cgtatctcac tgcgaccaac
     2821 caggccacag cccaagggat tatttacgcg ttgctggcca cgcatccggg agattcagct
     2881 ttgctggcgc gcgcagtgac caccttcgcg gactaccagt gttactccga cgcaatccgc
     2941 ctcactgacc accagttgca gcttacgccc gacgacccag cggccctggc taacaagggc
     3001 attctttgcg tcctgactgg ggattcctcg aacgccatac caacgctcac gcgctcgctg
     3061 tccttgacga atacttacgt ggcccggctc aatcgcgcca tcgcctattt gcgaacgggc
     3121 cgcctggacg cggcggaggc cgactaccag gcgctcctcc agcaatttcc caccgcttac
     3181 cgcgtcagcg ctatgctggg tgaggtcgcc ttgcagagga aggacaccaa cacggcgatc
     3241 cgatattacg agcagtacct ttccaagctt gagcccgaca ctgaagaggc caggtttgtc
     3301 gccgtgcggc ttaagtcgct ccagcaaggc gggccttgag ttctgccttc ggctgctcgt
     3361 tgctggaaat ggggcgattc cggcccgcgg gcagcgtggt gagcgcaccc agcggatcac
     3421 gcctgagcgg cgagactgcg gccggcgccg tgggcgcgtt ggtccgccgt tcgaagtcca
     3481 cccagacggc atcgttcatg tactcccggg cataggcgta gggcatccag ccatcctgcc
     3541 cgctgcggct cgtgttgcgg aagatgaaca ctcctccacc cggctgggcc gcatcgtccc
     3601 ggtaacccac cagcaagacg ctgtgtccgt cacgaacggc atttgaagga cacatttgca
     3661 gcacgttatc cac
//
LOCUS       DATCGS010000340         4545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_78868, whole genome shotgun sequence.
ACCESSION   DATCGS010000340 DATCGS010000000
VERSION     DATCGS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78868"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..762
                     /locus_tag="VIL39_06950"
     CDS             94..762
                     /locus_tag="VIL39_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481199.1"
                     /translation="MKILDIPQSGKRGLNVSQAGQFGQISRTLAIPANPRTPAQMTVR
                     DNLSRVAAKWRVLTEPQRVEWMAAASAVKSNSRLGQSGALSGFQLFAKINCTLAQFGQ
                     EQVDTPPLRPQFADLAPQNLVITNTGGVVALKLTCPTDPGENTIVRGSKPVSQGVEVC
                     KDFRILGTCPAAVAGSSDITGLYTARYGVPKAGTKVYVLVNQFVDGWESLPRTYSAIV
                     PAAA"
     gene            899..1564
                     /locus_tag="VIL39_06955"
     CDS             899..1564
                     /locus_tag="VIL39_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481200.1"
                     /translation="MKLKHISRNEKDASAVVCAGRYGLVSRRYVIPSNPRTERQMEVR
                     WHLAEQARRFGDLTDAQHEAWNAAAATYRSHPRQGQSGPLTGLHLFIRVNCKLALFGL
                     EPLNTPPPPPVFPELAPQDLVITNTAGVIAVKLVCPADPGDNTVLRASPPQSAGVGTC
                     REFRIIGMCPAPVVGLADITALYTACFGPPPVGRRIFLRASTMVSGFESLPREFRARV
                     PAS"
     gene            complement(1726..3144)
                     /gene="gltA"
                     /locus_tag="VIL39_06960"
     CDS             complement(1726..3144)
                     /gene="gltA"
                     /locus_tag="VIL39_06960"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEY5481201.1"
                     /translation="MSPKLSTKQRLQINRQKMTEQEPLVRNANFDEVNLGLPEKAALL
                     EAERCLQCKDPKCITGCPVSVNIPRFIALLSQGNLPDAAHCLLNDNALPAVTGRVCPQ
                     ETQCEIECIRGNKGLPVAIGYLERYVGDWARGHSNQQTLQIASPSGKKVAVVGSGPGG
                     LTAAGELVKYGHDVTVYEALHKPGGVLVYGIPEFRLPKNIVAQEVARLQQAGVKIVCN
                     TVIGRTYTLPELREQFDAVFIANGAGLPVFMNVPGENLKGVYSANEYLTRVNLMAAYQ
                     FPRSDTPVLKGQRIATVGGGNVAMDSARTAKRLGAESSIIVYRRTRKEMPARAEEIHH
                     AEEEGLQFEFLVAPVEVLGNEKKWVTGLKCVRMQLGEPDASGRARPVVIPGSEFVLAC
                     DVVVVAVGTRANPLLTATCPDLKLNKWGNIVVDDKGMTSLPGVFAGGDIVRGAATVIL
                     AMGDGKRAAKAMDEYLRRVQTA"
     gene            complement(3197..4036)
                     /locus_tag="VIL39_06965"
     CDS             complement(3197..4036)
                     /locus_tag="VIL39_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEY5481202.1"
                     /translation="MHSIVAKAQLSPNVTRLDVVAPRIAQIRQPGQFVIVHRAEGAER
                     IPLTIADADTRAGTIALVIQSVGKSTQELVALKPGEAIADIAGPLGHPTELIASGRAL
                     CIGGGVGTAVVHPIAQGLHRRGVDVLSIIGGRSKEWVIFEAELARFGQVTDCTDDGSY
                     GRHGFVTDAAKDALAAGGIDIVYAVGPVPMMRAVVNLTKPLGVRTTVSLNPVMVDGTG
                     MCGGCRVRVGGQTLFACVDGPEFDGHQVDFDLLMDRLSTYRDFEQRALAACQAGTCKI
                     GLK"
BASE COUNT          859 a         1438 c         1449 g          799 t
ORIGIN      
        1 acacaatttt ggcgcgcgca acctggctgg cgttgccggc cacgcgcgcc gcagcaagaa
       61 gaaaacaaca aaagaaagga aagtagcaga cgtatgaaaa tactagacat cccacaatca
      121 ggtaaacgtg gcctgaacgt ctcgcaggct ggccagttcg gccagatcag ccggacgctc
      181 gccatccccg ccaatccgcg caccccagcg caaatgaccg tgcgcgacaa tctcagtcgc
      241 gtggcggcca agtggcgagt gctcaccgaa ccccaacggg tcgagtggat ggccgcagcc
      301 agcgcagtca agagcaacag ccgcctcggc cagagcggcg ccctcagcgg cttccagttg
      361 ttcgccaaga tcaactgcac gctcgcacag ttcggccaag agcaggtgga cactccaccg
      421 ctccggccgc agttcgcgga cttggcgccg cagaacctgg tcatcaccaa cacgggggga
      481 gtggtcgccc tgaagctgac ctgcccgacc gatcccggcg agaacaccat cgtccgcggc
      541 tccaaaccgg tcagccaggg cgttgaggtc tgcaaggact tccgcatcct ggggacgtgt
      601 cccgcggccg tggcgggctc gtccgacatc acgggcctct atacggcccg ttacggggtg
      661 ccgaaggccg gcaccaaggt gtacgtcctg gtcaaccagt tcgttgatgg gtgggagagc
      721 ctgcccagga cctactcggc catcgtcccg gctgcggcct gagcatccgc cgggcgtgcg
      781 ggcggttgag caattgcccg ctcccggcgt ccaggcgggt gggtgtcctt cagggcgccc
      841 gcccgctctg gccccggccc cggtctcgta tccaccattt aagaaagaaa agcaacgtat
      901 gaaactaaaa cacatctccc ggaatgagaa agatgcttcg gcagtcgtgt gtgctggccg
      961 ctatggcctc gtaagccgcc gctatgtcat tccgagcaac cctcgaaccg aacgccagat
     1021 ggaagtccgc tggcatctgg cggagcaggc gcggcgcttc ggtgatctta ccgacgcgca
     1081 gcacgaggcc tggaacgcgg ccgccgccac gtaccggtcg catccccggc aaggccaaag
     1141 cggtccgctg acggggctgc acctgttcat cagggtcaac tgcaagctgg ccctcttcgg
     1201 tctggaaccg ctaaacacac cacctccgcc gcctgtgttc ccggaactgg cgccgcagga
     1261 cctcgtcatc accaacacgg caggcgttat cgcggtaaag ctggtctgtc cggcagaccc
     1321 gggtgataac acggtgttgc gggcgagccc accgcaatcg gccggcgtag gaacctgcag
     1381 ggaattccgc atcatcggca tgtgcccggc gccggtggtc ggtttagcgg acatcacggc
     1441 gctctacacc gcttgcttcg gcccgccgcc ggtgggtcgg cgaatcttcc tgcgggcgag
     1501 cacgatggtg agcggattcg agagcctgcc ccgtgagttc cgcgcccgag tgcccgcctc
     1561 gtgaccgcgg gtgtgcttct ctctcccggg gcaggcaggg acagctccgc acgggcggcg
     1621 gccacggact agactccagg ccgccgccct tgtttcgtaa agtcttatgc ggagcggggt
     1681 ccaaggactg tgtcgtcagc ccgccgcttg ggcaagcggc tgccgctaag ctgtctgaac
     1741 gcggcgcagg tactcgtcca tcgccttggc ggcgcgcttg ccgtcaccca tagcgaggat
     1801 caccgtggcg gcgccgcgga cgatgtcgcc gccggcgaag acgcccggga ggctggtcat
     1861 gcccttgtcg tccaccacga tattgcccca cttgttgagc ttgaggtctg ggcaggtagc
     1921 ggtgagcagc gggttggctc gcgtgccgac ggcgacgact acgacgtcgc aggccaggac
     1981 aaactccgag ccgggaatga cgacgggccg ggcgcggcca gaggcgtcgg gttcacccag
     2041 ttgcatgcga acacacttga ggccggtgac ccacttcttc tcgttgccga gcacttctac
     2101 cggggccacc agaaactcga attggagacc ttcctcttcc gcgtggtgaa tttcctccgc
     2161 ccgggccggc atttccttgc gggtgcggcg atagacgata atcgaggact cggcgcccag
     2221 gcgtttggcg gtgcgggcgg agtccatggc gacgttgccg ccgccgacgg tggcaatgcg
     2281 ctggcctttg aggacggggg tatcagaccg ggggaactgg taggcggcca tcaggttcac
     2341 gcgggtgaga tactcattgg cggaatagac gcccttgagg ttctcgcccg gcacattcat
     2401 gaagacgggc aggccggcgc cgttggcgat gaacactgcg tcgaactgtt cgcgcagttc
     2461 gggcagggtg taggtccggc caatgacggt gttgcagacg atcttcaccc cggcctgctg
     2521 cagccgggcc acttcttgcg caacaatatt cttgggcagg cgaaactccg ggatgccata
     2581 gaccagcacg ccacccggct tgtgcagggc ttcatagacg gtgacgtcgt ggccatactt
     2641 gaccaattcg cccgcggccg tgagaccacc cgggccggag ccaaccaccg ccaccttctt
     2701 gccgctggga gaagcaattt gcagggtttg ctggttgctg tggccgcgcg cccagtcgcc
     2761 gacatagcgc tcgaggtagc cgatcgccac cggcaagccc ttgttgccgc ggatgcactc
     2821 gatttcgcat tgcgtctcct gggggcagac gcggccagtg accgccggca aggcgttatc
     2881 gttgagcagg cagtgcgcgg cgtcaggcag gttaccttgc gacagcagtg cgatgaagcg
     2941 cgggatgttg acgctcaccg ggcagcccgt aatgcacttg gggtccttgc actggaggca
     3001 acgctccgct tcgagcaagg ctgccttctc aggcaggccg aggttgacct cgtcgaaatt
     3061 ggcattgcgg acgagcggtt cctgctccgt catcttctgg cggttgattt ggaggcgctg
     3121 cttggtagag agcttgggag acacgggaat gcgttggatg agttacaaga gttaaacggg
     3181 ttacatgagg acagacttac ttcagcccaa tcttgcaggt gcctgcctgg caggcggcga
     3241 gagcgcgctg ctcgaagtcc cggtaggtgg acaaacggtc catcagcaaa tcgaaatcca
     3301 cctggtgccc gtcgaactcg ggaccatcaa cgcaggcgaa aagggtctga ccgcccactc
     3361 tgacacggca cccgccgcac atgcccgtgc cgtccaccat caccgggttc agcgagacgg
     3421 tcgtgcgcac gcccagcggc ttcgtcaggt tgactaccgc gcgcatcatc gggaccgggc
     3481 cgaccgcata aacgatatca atgccgcccg cagccagcgc gtctttggcg gcatccgtca
     3541 caaaaccatg ccgcccgtag ctgccgtcgt cggtgcagtc ggtcacttga ccgaaccgcg
     3601 ccagctccgc ctcgaagatg acccactcct ttgagcgccc gccgatgatg ctgagcacgt
     3661 ccacgccgcg gcggtgcagg ccttgggcaa tgggatgcac cacagcggtg ccgacgccgc
     3721 ccccgatgca gagcgcgcgg cccgaggcaa tcagttccgt gggatggccg agcggtccag
     3781 cgatgtcggc aatggcctcg cccggtttca gcgccaccag ttcctgggtg ctcttgccca
     3841 ccgactggat gaccagggca atggtgccgg cgcgggtatc cgcgtcggca atggtcagcg
     3901 ggatgcgctc cgcgccctcg gcccggtgca caatgacgaa ctggcccggc tgccggattt
     3961 gcgcaatgcg cggggcaacg acgtccagcc gggtgacgtt agggctgagc tgggctttgg
     4021 caacaatgct atgcatatcg ggctctcgaa ttcataaacg acaaaatcgt ggcggatgac
     4081 tcgcctggaa gtcaactgtc tttgtcaact gtcttgcggc cgcgggcagc aacaagggca
     4141 ggggcaaagt ctatcccggg ccggagcgcc ggcgctccgc gactttgccg ctagccccgc
     4201 gtgcgtcagc ggctggagca aatggcgtgt ttgacttttg ttggggccag cccttttgac
     4261 aagtctgttg catggtgtac attagcacat cagatatgga tttgggcaat agaacaaatt
     4321 acagccgtcg gagtggtctc aaatgtatag aagaaactga acgaaagcag atagcttgcg
     4381 ctccaaacgg tcgacggagc cgccaaccat tgaaacacca agacaggaga taattatgtg
     4441 caatagtaac atccaaatat cgcaaacaaa ccccaagcta agatcacttc ggtccgggat
     4501 gcaaatccgc ctggtctcca ggctgctgcc ggcgttcctc gtgct
//
LOCUS       DATCGS010000341         5231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_276795, whole genome shotgun sequence.
ACCESSION   DATCGS010000341 DATCGS010000000
VERSION     DATCGS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276795"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1591
                     /locus_tag="VIL39_06970"
     CDS             266..1591
                     /locus_tag="VIL39_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY5481203.1"
                     /translation="MKSRIFFWSITSALAGFLFGFDTVVISGAEQKIQSLWGLSAGLH
                     GVAMAAALYGTVLGSMLGGWPTDRFGRKATLLWIGVLYLVSAVGCAFATGVSSFIAAR
                     FIGGLGIGISTVAAPLYISEIAPPAYRGRLAGMFQFNIVFGILIAFLSNALLAGIGEN
                     AWRWMLGVAAFPSLLYTLLCFGIPESPRWLLGRKRDREAGLKVLRLIRPEASPAELEG
                     QADEIMAASSEQTTAARFWTRRLRVPILLAVLIAFFNQLSGINAVLYFAPRIFEMTGL
                     GAKAALLQSVGIGVTNLIFTFVGLWLIDRLGRRTLLFIGSFGYIFSLGLTSWAFFTGH
                     FSIVPICIFGFIAAHAVGQGAVIWVFIAEIFPNRHRAEGQALGSFTHWLFAALLTSFF
                     PKMVTAFAPGYVFLFFCAMMVLQLVWVKTMVHETKGVPLEQIQKRLGIE"
     gene            complement(1600..4575)
                     /locus_tag="VIL39_06975"
     CDS             complement(1600..4575)
                     /locus_tag="VIL39_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 38"
                     /protein_id="HEY5481204.1"
                     /translation="MSLMLKQKYLPVALLVFGLACRFGVGVSGAENTPSAVSNIQSLS
                     LAVSPLIPKVAQGERNILVASIDSSGLTNVAVTLTSPDWPQVARQTIPSLPKGKQSVE
                     IQVAPLTGPTPVTVRVESAGLSREFGPFTVLPPRKWTVYLTQHTHTDIGYTRPQTEIL
                     PEHLRYIDYALDYCDLTDSLPDDARFRWTCETSWAVREYLRDRPAVQIARLKQRVREG
                     RIEICGLLLNMSEVATENSLAALVRPVRAITEEFGITVRTAMQDDVNGAGWCLADYLN
                     GIGIRYLNMGINKTRSLLPFDQPTCFWWESPSGKRVLAYRGEHYMTANFWGVEKGDAG
                     LIKPKVADYLTSLEGLHYPFDRVGVQFSGYFTDNSPPSTAACNLVKVWNEKYAWPKLR
                     LSTAQEFLAWVEKEQGSKLAVHRQAWPDWWTDGFGSAARETAAVRETETAMDVNHRLL
                     AMARLLGAPIHAPTSQRAAAIMDNLLFYEEHTFGAAESISDPLAENAQVQWGEKASYA
                     WSAVKDSALLREEAFGLVQSFLPRSDVPTLAVFNTLNWTRSGMVRVFIDHQILPPDRE
                     FRILDGADSVLAQEMERRTEGTYWALWVKDIPPLACKILRLEAGDKKRAKEASAKDSV
                     SLENAFYKLTLDPVKGSIQSLRDKETGAELIDPGANWGFGQCVYETMPANREMKPEVF
                     KRTAWHNVKASPGAVGPIWHSLRLSADLDGCATNKGAQVEIRLYDTEKRVELHFAIRK
                     LPVPTPEAVYVTLPFQSADGKLLYEGQGGLVTPGEGQIPGSSSDWQTIQSFLSIRNAA
                     GQIIVGSEQAPLVQLGDFNLGKWMPITQIAKPHVYSWVMNNYWFTNFRTEQEGEFKWH
                     YYLTSTKDVSTATATRFGWSSRVPLVTRVLPAAKGKAAARGASSATGSTVIPVMAFPA
                     QNLLVVEYRPAADLKGIVLHLREVAGQPASLARKDIVSAARISRLDEVNVLEQPLKTK
                     LDTVTFQPWETKFLKLSLR"
BASE COUNT          914 a         1514 c         1743 g         1060 t
ORIGIN      
        1 tcgagggagg agatcctggc cgccctggcg gacgtaagga cccagtcgca atcctcccct
       61 ggcggccctg agggcaacaa cgcctgagcc cgtaagctac gaccaccgag cgcggcctgt
      121 tccgttttcg cggatgggag gcatcgccgc tttcagatgc ttgctcgaaa gcactgttat
      181 ccgccggctc attccaccag cagaacacaa cagaaacccc agatatttgg cttgtaacgg
      241 gggccatttt acgcgaaaag gtcgcatgaa gagccgcatc tttttttggt ccatcacctc
      301 cgccctcgcc ggattcctat ttggcttcga tacggtggtc atttccgggg cggagcagaa
      361 gatccagtcg ctgtggggcc tcagtgccgg gctgcacggg gtggcgatgg ccgcggcgct
      421 ctacggcacc gtcctgggtt caatgctcgg cggttggccc acagaccgct ttggacgcaa
      481 ggccacgctg ctgtggatcg gcgtgcttta tctcgtttcg gcggtcggtt gcgcgttcgc
      541 cacgggggtt tcctcattca tcgcggcgcg gttcatcggc ggtttgggga tcggcatttc
      601 caccgttgcc gcgccgctct acatctccga aatcgcgccg ccagcctacc gtggccggct
      661 ggccggcatg ttccaattca acatcgtctt tggcatcctc atcgcgttcc tgtccaatgc
      721 gctgctcgcc gggatcggcg agaatgcctg gcgctggatg ctgggcgtcg cggcgtttcc
      781 gtccttgctt tacacgttgt tgtgcttcgg cattccggaa agcccgcgct ggctcctggg
      841 ccggaagcgc gaccgcgagg cggggctgaa ggtgctccgg ctcatcagac ccgaggcatc
      901 cccggcggag ctcgagggcc aggcggacga gatcatggcc gcgtcctccg agcaaactac
      961 cgcggcgcgt ttctggaccc ggcggctaag ggttcccatc ctgctcgcgg ttctgatcgc
     1021 gttcttcaac cagctctcgg gcatcaacgc cgttctctac ttcgcgccac gcatcttcga
     1081 gatgaccggg ctgggcgcca aagccgcctt gctgcaatcg gttggcatcg gggtgaccaa
     1141 cctgatcttt acctttgtcg gcctctggtt gattgaccgg ttgggacgcc gcacgctcct
     1201 gtttattggc tccttcggtt atatcttctc gctcgggctg acgtcgtggg ctttcttcac
     1261 cgggcatttc agcatcgtcc cgatttgcat cttcggcttc atcgcggcgc atgccgtcgg
     1321 gcagggggcg gtgatctggg tgttcatcgc cgagatattc cccaaccggc accgggcgga
     1381 aggccaggcg ctgggcagct tcactcactg gcttttcgcc gcgctgctca cgagcttctt
     1441 tccgaagatg gtcacggctt ttgccccggg ctatgtcttc ctgttcttct gtgccatgat
     1501 ggtcttgcag ctcgtctggg tgaagaccat ggtgcacgag accaaaggcg tgccattgga
     1561 gcagattcaa aaacggctcg ggattgagta acgctcgcat tacctcaaac tcagcttgag
     1621 gaacttggtt tcccaaggct ggaaggtgac ggtatccagc tttgtcttga gcggttgctc
     1681 gaggacgttg acttcgtcga ggcggctgat gcgggcggcg gagacgatgt ccttcctggc
     1741 aagggaggcg ggctggccgg cgacttcgcg caggtgcagg acgatgccct tgaggtcggc
     1801 ggcggggcgg tattcgacca cgagcaggtt ctgcgcgggg aaagccatca caggaatcac
     1861 cgtggagcca gtggcgcttg aagcgccacg ggcggcggct ttgcctttgg ccgccggtag
     1921 aacgcgggtg acgagcggga cgcgggaact ccagccaaag cgcgtggcgg tggcggtgga
     1981 gacgtctttg gtggaagtca ggtagtaatg ccacttgaat tcgccttcct gctcggtgcg
     2041 gaagttcgtg aaccagtagt tattcatgac ccacgaatag acgtgaggct tggcgatctg
     2101 ggtgatgggc atccatttgc cgaggttgaa atcgcccagt tgcacgagcg gcgcttgttc
     2161 gctgccgaca atgatctggc cggcggcgtt gcggatggag aggaagctct ggatggtctg
     2221 ccagtcggaa gatgagccgg gtatctggcc ttcgccaggg gtgacgaggc ccccctgtcc
     2281 ttcgtaaagc agtttgccgt cagccgactg gaacggcagg gtcacataga cggcttccgg
     2341 ggtgggcacc gggagcttgc gaatggcgaa gtgcagttcg acgcgcttct cggtgtcata
     2401 caagcgaatc tcaacctgcg cgcccttgtt ggtggcgcag ccgtccaagt cggcgctcag
     2461 acggagactg tgccagatgg ggccgaccgc gcctggactg gccttgacgt tgtgccaggc
     2521 ggtgcgcttg aagacttcgg gcttcatctc gcggttcgcc ggcatcgttt catagacgca
     2581 ctggccgaaa ccccagttcg cgcccgggtc aatgagttcg gcgccagtct ctttgtcgcg
     2641 gaggctttgg attgagcctt tgacgggatc gagcgtcagc ttgtagaagg cattttcaag
     2701 gctcacgctg tctttggcgg aagcctcctt ggcacgcttc ttatcgccgg cttcgaggcg
     2761 gaggattttg caggccagcg gaggaatgtc cttcacccac agcgcccaat aggtgccttc
     2821 ggtgcgacgt tccatctcct gcgcgaggac tgagtcggcc ccgtcgagga tgcggaattc
     2881 gcggtcgggc gggagaatct ggtggtcaat aaacacgcgg accatgccgg agcgcgtcca
     2941 gttcagcgta ttgaagacgg ccagcgtggg aacatcggag cgcggcagga agctctgcac
     3001 caggccgaac gcttcctcgc gcaacagggc ggagtctttg acggcactcc aggcataaga
     3061 ggctttctcg ccccattgaa cctgggcgtt ctcggccagg gggtcgctga tgctctcggc
     3121 ggcgccgaag gtatgttcct cgtagaagag gaggttgtcc atgatggcgg cggcgcgctg
     3181 actggtcggc gcgtggatgg gcgcgcccag cagccgggcc atggcgagca gacgatggtt
     3241 gacgtccatg gcggtctcgg tctcacggac ggcggcggtt tcgcgagcgg cggagccgaa
     3301 gccatccgtc caccagtcgg gccacgcctg gcgatgcacg gccagcttgc tgccctgctc
     3361 cttttcaacc caggcgagga actcctgtgc cgtcgagagc cgaagtttgg gccaggcgta
     3421 tttctcattc cacaccttga cgaggttgca ggcggcggtg gagggcggcg agttatccgt
     3481 gaaatagcca gagaactgca cgccgacgcg gtcgaacgga tagtggaggc cttcgagcga
     3541 ggtgaggtag tcggccacct ttggcttgat gagcccggcg tcacccttct cgacgcccca
     3601 gaagttggcg gtcatgtagt gctcgccgcg ataggcgaga acgcgtttgc cggagggaga
     3661 ttcccaccag aagcaagtgg gctggtcgaa cggcaggagc gagcgggtct tattgatgcc
     3721 catgttgagg taacggatgc cgatgccgtt caggtaatcc gccaggcacc agccggcgcc
     3781 gttgacatcg tcctgcatgg cggtgcgcac ggtgatgccg aattcctcgg tgatggcgcg
     3841 cacggggcgg accagcgcgg cgagcgagtt ctccgtggcc acctcggaca tgttcaagag
     3901 caggccgcaa atctcgatgc gcccttcgcg gacgcgttgc ttgagccggg cgatctggac
     3961 ggcggggcgg tcgcggagat attcgcgcac ggcccaggaa gtctcgcagg tccaacggaa
     4021 gcgggcgtca tccggcaggc tgtcggtcag gtcgcagtaa tcgagcgcgt aatcaatgta
     4081 gcgcaggtgc tcgggaagta tctccgtttg gggccgggtg tagccgatgt cggtgtgggt
     4141 atgttgcgtc agatagactg tccacttgcg gggtgggagc accgtaaagg gaccgaactc
     4201 gcggctcagc ccggccgact cgacgcgcac ggtgacagga gtggggccgg tcaagggcgc
     4261 aacctggatt tccaccgact gcttgccttt gggcagggag gggatcgtct gtctcgcaac
     4321 ctgcggccag tccggcgagg ttagggtcac cgccacattg gtcaggccgc tggagtcaat
     4381 ggaggcgacc aggatgttgc gttcgccctg ggcgactttg ggaatgagcg gcgagaccgc
     4441 caggctcagg gattgaatat tgctgacggc ggacggggtg ttttccgccc cggagacacc
     4501 aacgccaaaa cgacaagcca gaccgaagac aagcagggcg acggggagat acttctgttt
     4561 aagcatgaga ctcatttgag gccattcaga ggcaacatgc aagcctcgga aaggtatgat
     4621 cgagggctgg aatgcctggg agcgctcagg cgctgaggga tgagggagcg ccaaaaggag
     4681 ccgggaaggg aaaactgagc tgcgcgatgt cctcgactga tctgctgcgt ccgcgcaaac
     4741 gacgtgcctg cgggcggaca ggttcgtctc ctctgccggg aaggctgctt tgcagcttga
     4801 tcctcatctc gcgaaaatga cgggggcaaa tcccatgcga cagggcaccc aaggtcgcgc
     4861 agagctcccg gggcttgcac caggcacaga ccgcgacgcg cagcgggaac tcagtctgag
     4921 ccgctgcgct ctcgctccca ggcttacctg ggtgttccaa atagcgcatg caagtcaggt
     4981 catacaagaa ttgagcccac taagcaaaca ggaatcgcat caatgccatc tcaaccggcg
     5041 aggacctctc tggcccgtga gcagatgtga gtaccttcca gagcgctctg accgctgctg
     5101 gctccttgtg gctcaggcag cgcctgattt ccccgcgccg ttcagagcgt gactgacgat
     5161 cgtctgtgcc tcgctgacaa tggcttccaa gtgcttctga tcgcggaagc tctccgcata
     5221 aatcttgtag a
//
LOCUS       DATCGS010000342         9042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_224805, whole genome shotgun sequence.
ACCESSION   DATCGS010000342 DATCGS010000000
VERSION     DATCGS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224805"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="hisA"
                     /locus_tag="VIL39_06980"
     CDS             complement(<1..527)
                     /gene="hisA"
                     /locus_tag="VIL39_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HEY5481205.1"
                     /translation="MKQIVGGTLNAGPGRLQTNFVSDRPAAWYAELYQRDGLKGGHVI
                     MLGPGNDTEAQAALQAYPGGLQIGGGVNLNNARAWLDAGASHVIVTSWVFRGGQVDWE
                     RLAQLVEAIGKARLVLDLSCRRRGGGYFVVTDRWQKFTELALSCESLEKLAAWCAEFL
                     VHAVDVEGQCRGIDQ"
     gene            1132..2151
                     /locus_tag="VIL39_06985"
     CDS             1132..2151
                     /locus_tag="VIL39_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5481206.1"
                     /translation="MNHTTVSAARVRQILTGASQTRVLVVGDAMLDHFIRGSVARISP
                     EAPVPVVDFEGESFMPGGAANVARNLTALHVPTELLGAVGRDPAATQLKELLSQYHIG
                     CRGLLVDTARSTSVKTRIVAHKQQVVRIDRETRDGLNTSLTRRLASTVQSLLPGVAAV
                     IVGDYGKGVVTQPLLDEVKRICRAHGVWLSLDPKPVHHLNLSGLSLITPNRKEAFELA
                     GLADETRHNNPLADANLLRVADRLLNELRPAVLLITLGELGMLLCRHGHKPFHIPTVA
                     QEVFDVSGAGDTVIASFTLAIAAGASPLEAAILSNHAAGIVVGKIGTATVAPEELLAS
                     FKLRR"
     gene            2203..2979
                     /gene="panB"
                     /locus_tag="VIL39_06990"
     CDS             2203..2979
                     /gene="panB"
                     /locus_tag="VIL39_06990"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5481207.1"
                     /translation="MTGIKVTAETIRAMKGRGEKIAALTAYDCPMTRLLDEAGVPLIL
                     VGDSLGMVVLGYPDTTHVTMAEMEHHVRAAARAKPCALLAADLPYHSYETVADAVANA
                     KRLAAAGAEAVKAEGGTEILEQVRAIVGSGIPFLGHLGMLPQHVLEEGGYHVKGKSEP
                     EHQRLLADARALAAAGVFAVVLELVTPRVAKDLTQQLPIPTIGIGAGPDCDGQVLVTP
                     DLLGMLPWYNLKHVKPKLNAAEQMRAVVKAWKESVEAVKG"
     gene            2997..3449
                     /gene="moaC"
                     /locus_tag="VIL39_06995"
     CDS             2997..3449
                     /gene="moaC"
                     /locus_tag="VIL39_06995"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5481208.1"
                     /translation="MKKLTHIDTRGEASMVDVSAKPVMFREATASGEVRLQRATLKLI
                     ESQTIAKGNVLAAARLAGILAAKKTGELIPLCHPLPLTHCEVHFAIPRTRDRIVITST
                     ARIAAQTGVEMEALTAVSVAGLTIYDMCKAVDKTMRITQVKLVSKTKR"
     gene            3552..4034
                     /locus_tag="VIL39_07000"
     CDS             3552..4034
                     /locus_tag="VIL39_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5481209.1"
                     /translation="MQIQVGIITISDRASQNVYDDRGGPALKAAAEAYGWKVLAEALV
                     PDEKQDVQRAVREQVARGCQLILTTGGTGAARRDVTPEAVREIGGRELPGFGEVMRLE
                     SLKLTPNAILSRSLAIVVEQTLVICLPGKPKGAVECLGFVAGAIPHCVEILQEASANS
                     "
     gene            4091..5296
                     /locus_tag="VIL39_07005"
     CDS             4091..5296
                     /locus_tag="VIL39_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5481210.1"
                     /translation="MDLDSLVNLAATNGASDLHLEAGLPAAMRVRGTLRTTGEPIPAN
                     LLLDMARQVTGEEEWPQFLEQKSHDCSRNIHGVRVRINILQTSRGIGFAIRLLATFVA
                     TVDKLNLHPDFKKLITGHNGLILVSGATGSGKSSTMAALIQEINMTETRHIVTIESPI
                     EYTFRPRRAYIRQREVGRDTPSFEQALIDSLREDPDVLMVGEMRDPETMRLTLSASET
                     GHLVMATVHSSTCAEALQRVVSAFPSEIQSNVASQLADCVVAVISQRLRFRPELNIRV
                     PECEILVPTHAVKNFIRNRDFFKITSSLETGAEHGMWTLQRYRAWLDGRKNWFVPGQI
                     PEASDGEPGEAAPPPAHPPAFPLAPKPSKFETRHGSLPQTHEAKTSTRIEIEPGESEF
                     GKILKQPGE"
     gene            5299..5721
                     /locus_tag="VIL39_07010"
     CDS             5299..5721
                     /locus_tag="VIL39_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481211.1"
                     /translation="MVAKRSTEPSRRAIFKVWGTLLVAVALGGCVSKGKANAQARAAF
                     MAGQQQAMARMQQAQGPSVTINGEVRNHVVPWTEGLTLAKAVLAADYCGAKDPGQLLI
                     VHNGIATHVDPKQLLSGVDIPLQPGDIVQLVPQAAPLK"
     gene            5780..6871
                     /locus_tag="VIL39_07015"
     CDS             5780..6871
                     /locus_tag="VIL39_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY5481212.1"
                     /translation="MTEPEPLQQINRTYVRCRQRKLSYFSGCDYFRLASHPRVMAALQ
                     AGVKKYGLNVAASRMTTGNHVLYRELEAQLADFFAAESALLVSCGYVTNLVVAQALAG
                     HFSHALIDAAAHCSLADAARLLDCPVLEFKSRDPEALALAVKRCGPESKLLVLTDGMF
                     SSDGSAAPLAEYLKILPKDALILVDDAHGAGVLGRNGQGAPEHAGVSRRRVIQTVTLS
                     KSFGAYGGAILGSARLRQQILDRSRMFVGSTPLPLPLANAALAGVRILKTDKSLRQRL
                     VQNAAYMKAALREAGLPLAETPGPIVPLHPRRPSEATRLNRALLAAGVYPPFIKYPGG
                     PASGYFRFVISSEHSRSQLDAVLSVLLAS"
     gene            6919..8274
                     /locus_tag="VIL39_07020"
     CDS             6919..8274
                     /locus_tag="VIL39_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481213.1"
                     /translation="MALWPIPGEKAPRAKLCGGRRTTSTLFGLMLSLLASHAASSFQV
                     TAFALDGLLTWTNAPVPGICTVEAASTLAGPWTPGQNAFATNSADTLTVPITGGTRFY
                     RMQGVAVPPTPQGFTNLVYAYGILETIAGNGLGRLDTSNYWSAAYEGGPATLAALSRP
                     HIAMADRAGNVYIADKNSHSVLRVAPDGTIHTHAGTHTGGFNGEGPAPSTTLQLNFPN
                     GLWVRSDGTVYVLDTDNGRVRRVSTNGIMTTLFLATSDGSALNTGRGLWVKDDEALAY
                     FCAGTKVRSWTSTNNLSTLASGFSDLGDLFVEPGGDVIVCDRGANYAYRVHPNGSSVV
                     LAGNGNTNGGGDGFSALATGLYGVRSPWPVPTGGYLLLSHEGCQLWYLDSAGIIHLLL
                     NGSGGLTHDGDGAFFYDPSVPKISEGRSVTMDYDDNVLICESDYGYIRRIRFQRIPPA
                     S"
     gene            8471..>9042
                     /locus_tag="VIL39_07025"
     CDS             8471..>9042
                     /locus_tag="VIL39_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HEY5481214.1"
                     /translation="MVRPAHYSGMCDASGAVAVSSNLFVVANDEDNVLRLYRSDQPGQ
                     PVKQFDFNAFLEVQGKSLEADLEGAARIGDRAFWIGSHGRNKDGKERLNRHRLFATDI
                     SVNAGEVALTAVGTPYRLLLDDLLRDARFDQFHFAEAAHRAPKDPDALNIEGLSAMAE
                     GQLLIGFRNPVPAGKALLIPLLNPNEVIQA"
BASE COUNT         1699 a         2902 c         2735 g         1706 t
ORIGIN      
        1 tcctgatcaa taccgcggca ctgcccttca acatccacgg cgtggaccag gaactcggcg
       61 caccacgcgg caagcttctc caagctctcg cagctaagcg ccaactcggt gaatttctgc
      121 cagcggtcgg tgaccacgaa atagcctccg ccgcgtcggc ggcagctcag gtccagcacc
      181 agccgcgctt tgccgatcgc ctcgacaagc tgggcgagtc gttcccaatc cacctggccg
      241 ccacggaaaa cccaggaggt gacaatgaca tgcgaagcgc ccgcatccag ccatgcgcgc
      301 gcgttgttga gattgacccc accgccgatt tgcagcccgc cgggataggc ctgcagtgcc
      361 gcttgcgctt cggtgtcgtt gcccggaccc agcatgatca cgtgcccgcc tttcagacca
      421 tcgcgctggt acagctcggc gtaccaggcg gccggacgat cggagacaaa gtttgtctgt
      481 aaccgtcccg gaccggcatt cagggtgccg ccgacgattt gcttcacttt gccctcatgc
      541 agatcaatgc acggacgaaa cacaccaaat cctaaaggct aaaggctgaa cgctaaaggc
      601 taaagtctgc tgcctcgtgc cccgggagca tctcatttct tgcacctcta gcatggagga
      661 cgcgcgttgc gtcacagaat ggctgcttcc acggtggaat tgggcctcgg gcgggcgggc
      721 tgccacggcc ctgccagcgt gttgctctcc cgctgctctg gcgatgcttc gaaggagtta
      781 cgatggagtt acgatggggc ttcgaaggag ttacgaaggg attcaaggga aatctgccgc
      841 ccgttacggt ctctcgactg tctcggcgac gcgatgagtc tgctcgaaac cagggcgcct
      901 tccggggctt ggatgccgct gtggcgtcgc agccccggct ttgacgaccg agcttcgcct
      961 gtgttcattc atgcagatca cagccgagct ccagcgggag cggtgagttc agtcctccag
     1021 cttcccgagc ccattgggac gggaaccctc ccccattttg ctctggcatt cccgggcggg
     1081 gttgtctttc ctatccgggt ccgtcggtgt tcagccctgg ctaaaacgaa catgaatcac
     1141 actacagtct ctgccgcccg cgtgcgccaa atcctgaccg gcgcaagcca aacccgtgtc
     1201 ttggtcgtcg gcgacgccat gctcgaccac ttcatccggg gcagcgtggc ccgcatttcg
     1261 ccggaagcgc ccgtgccggt cgtggatttc gagggcgaaa gctttatgcc cggcggcgcg
     1321 gccaatgtgg cgcgcaacct gaccgccctc cacgtgccca cggaattgct cggcgcggtc
     1381 ggccgcgacc cggcggcgac gcaactcaag gaactgctca gccagtacca catcggctgc
     1441 cgcgggctgc tcgtggacac cgcccggtcc accagcgtga agacgcgcat cgtggcccac
     1501 aagcaacagg tcgtccgcat tgaccgtgag acacgcgacg gcctcaacac ttctcttacc
     1561 cggcggctgg caagcacagt gcaatcgctg cttccgggcg tggcggcggt catcgttggc
     1621 gattatggca aaggcgtcgt cacgcagccg ctgctggatg aggtcaaacg gatctgccgc
     1681 gcccatgggg tctggctcag tctcgatccc aagccagtgc atcacctcaa cctgtccggg
     1741 ctctcgctga tcacgccgaa ccgcaaagag gccttcgagc tagccggcct ggcggacgag
     1801 acgcggcaca acaatccgct cgcagacgcc aacctcctgc gcgtcgccga ccgcctcttg
     1861 aacgaactgc gaccggcggt gctgttgatc accctgggtg aattgggcat gctgctttgc
     1921 cggcatgggc acaagccgtt tcacatcccg acggtggccc aggaagtctt cgacgtgtcc
     1981 ggggccggag atacggtgat tgcgtcgttc acgctggcga ttgcggcggg cgcatccccg
     2041 ctggaggcgg cgattctctc gaatcacgcc gccggcatcg tggtcgggaa gatcggcacg
     2101 gccaccgtcg cccccgaaga gttgctggcg agcttcaagc tgcggcgctg actcatccag
     2161 tttctgcttg tcgagtgctg cttttgccat gaaggttgcg ccatgactgg gattaaagta
     2221 accgctgaaa ccatccgcgc gatgaagggg cgcggagaga agatcgccgc cctgactgcg
     2281 tacgactgcc caatgacccg cctgctggac gaggcggggg tcccgctgat cctggtcggc
     2341 gattcgctgg gcatggtggt gctgggctat ccggacacga cccatgtcac catggcggag
     2401 atggaacatc acgtacgcgc cgcggcccgc gccaaaccgt gcgccctgct ggcggcagat
     2461 ctgccctatc acagttatga gaccgtcgcg gacgccgtgg ccaatgcaaa acgcctcgcc
     2521 gccgccgggg cggaagccgt caaggctgag ggcggcaccg agattctgga gcaggtccgc
     2581 gcgatcgtgg ggagcgggat tcctttcctg ggccatctcg gcatgctgcc ccagcatgtg
     2641 ctcgaagaag gcggctacca cgtcaaaggc aaaagcgagc cagaacacca gcggttgctg
     2701 gcggacgcgc gcgcgctggc agcagcgggc gtcttcgccg tcgtgcttga actggtcacg
     2761 ccgcgggtcg cgaaggacct gacgcaacag cttcccatcc caaccatcgg gattggcgcg
     2821 gggccggact gcgatgggca agtcctcgtc acgcccgact tgctgggcat gctgccctgg
     2881 tacaacctca aacacgtgaa gcccaagctc aacgccgccg agcaaatgcg agcggtggtc
     2941 aaggcttgga aggagagcgt ggaagcggtg aagggttgaa cggctgaagg gctggattga
     3001 agaaactgac acacattgat acgcgcgggg aagcgagcat ggtggatgtt tcagccaagc
     3061 cggtgatgtt ccgcgaagcc acggccagcg gagaggtgcg gctgcaaaga gccacgctca
     3121 agctcatcga gtcccagacc attgcgaaag gcaacgtttt ggccgcggcg cgattggcgg
     3181 gtatcctggc ggcgaagaag accggcgaac tcattcccct atgccatccc ctgcccctca
     3241 cccattgcga agttcatttt gccatcccca ggacccgcga ccggatcgtc attacctcta
     3301 cggccagaat tgccgcgcag accggcgtgg aaatggaagc cctcacggct gtgagcgtgg
     3361 ccggcctaac gatttatgac atgtgcaagg cggtggacaa aaccatgcgc ataacccagg
     3421 taaagctggt ttccaagacg aaacgctgaa gtggttacat gagttacaca gttacatgag
     3481 ttgcatcgat tcctgcttcc gcgccttatg taactcatgc agcaatgcaa cccgtgtgac
     3541 tcgttaagcc tatgcaaatt caggtcggca tcattacgat ctcagatcgc gcgtcgcaga
     3601 acgtttacga tgaccgcggc ggcccggcgt tgaaggcggc agcggaggct tacggctgga
     3661 aggtgctggc cgaggcgctg gtcccggacg aaaagcagga tgtccagcgc gcggtccgcg
     3721 agcaggtggc gcggggctgc cagctcatcc tcaccaccgg cggcactggt gcagcccggc
     3781 gcgacgtgac acccgaggcg gtgcgtgaga tcggcgggcg cgagctgcct ggtttcggcg
     3841 aagtcatgcg gctggaatca ttgaagctca ccccgaacgc catcctctca cggagcctgg
     3901 ccatcgtggt agagcagaca ttggtgatct gccttccagg caagccgaag ggagcggtcg
     3961 agtgccttgg ctttgtcgcc ggggccattc ctcactgcgt cgagatcttg caggaagcat
     4021 cggcgaacag ttagccggcc tgaagcagta actttttgcc acggcgcttg ttgaaggata
     4081 caatctacgc atggatctgg attcactagt taatctggcg gccactaacg gggccagcga
     4141 tttacacctg gaagcggggc tgccagcggc aatgcgtgtt cgcggcacgc tgcgcaccac
     4201 gggcgaaccc atcccggcca atctgctgct ggacatggcg cggcaagtga ccggcgagga
     4261 ggaatggccg cagttcctgg agcaaaagtc ccacgactgc tcacgaaaca tccacggcgt
     4321 gcgcgtccgc atcaacatcc tgcagacctc ccgcggcatc ggtttcgcca tccggcttct
     4381 cgctaccttc gtcgcgaccg ttgacaagct caatctgcat cccgacttca agaagctgat
     4441 caccggccac aacgggttga tcctggtcag cggcgccacc ggttccggca agtcctcgac
     4501 gatggccgcc ttgatccagg agatcaacat gaccgagacg cggcacatcg tgaccattga
     4561 aagccccatc gaatacacct ttcgaccgcg ccgcgcctac atccgccagc gcgaagtggg
     4621 gcgtgacacg ccgagcttcg agcaggcgct cattgattct ctgcgcgaag accccgatgt
     4681 gctgatggta ggcgagatgc gcgacccgga gacgatgcgg ttgacgttga gcgcttccga
     4741 gaccggccac ctggtgatgg ccaccgtcca ttcctcgacc tgcgctgagg cgctgcaacg
     4801 agtagtctcc gccttcccgt ccgagatcca aagcaacgtt gcttcccaac tggccgattg
     4861 tgtggtggcc gtcatctccc agcggctgcg gtttcggccc gagctgaaca tccgcgtccc
     4921 ggagtgcgag atactcgtcc cgactcatgc ggtaaagaac ttcatccgca accgcgactt
     4981 cttcaagatc acctcctcgc tggagacagg agccgaacac ggcatgtgga ctttgcaacg
     5041 gtatcgcgcg tggctggacg gccgcaagaa ctggtttgtt cccgggcaaa tccccgaagc
     5101 gtccgacggt gagccaggtg aagccgcgcc gcccccggcg cacccgccgg cctttcccct
     5161 ggcgcccaag ccctcgaaat tcgagaccag gcatggatcg ctgccccaga cccatgaggc
     5221 taaaacgtcc accagaattg agatcgagcc aggcgagagc gagttcggga agattctcaa
     5281 gcagccgggt gagtagtcat ggttgccaag cggtcaacag aaccttcacg aagggccatt
     5341 ttcaaggtct ggggcacgct gctcgtcgcc gttgcactcg gaggctgcgt ctccaagggc
     5401 aaggccaacg cacaggcgcg agccgccttt atggctgggc agcagcaggc catggcgcga
     5461 atgcagcagg ctcaggggcc cagtgtcacc atcaatggcg aggtccggaa ccatgttgtc
     5521 ccctggaccg aggggctgac tctggccaag gccgtgctcg cggctgatta ttgcggtgcc
     5581 aaggaccctg gccaactcct catcgtgcat aacggcatcg cgacccacgt tgatcccaaa
     5641 cagttgctga gcggtgtgga cattcccttg cagccggggg acatcgtgca gctcgtgccg
     5701 caggccgcgc cgctcaaata gtagccgcgc tctactttcg tcgtgctttg cgaggggagt
     5761 ttgcttatac catggggcca tgaccgagcc tgaaccgctt cagcaaatca atcgcaccta
     5821 cgtgcgctgt cgtcagcgca agctttccta tttctccggc tgcgactact tccgcctcgc
     5881 cagccatccg cgcgtcatgg cggcgttgca ggccggtgtt aagaaatacg ggctgaacgt
     5941 cgcggcttcc cggatgacta cgggtaacca tgtgctttac cgcgaactgg aagcgcaact
     6001 ggccgacttc ttcgccgcgg aaagcgccct gctggtctcg tgcggttacg tgaccaacct
     6061 cgtcgtcgcc caggcgttgg ctgggcattt ctcgcacgcg ctcattgatg cggcagccca
     6121 ttgctccctg gcggacgcgg cgcggctcct ggattgtccg gtactcgagt tcaaatcccg
     6181 cgaccccgaa gccctggccc ttgccgttaa gcgctgcggt cccgagtcga agctgctcgt
     6241 gctgaccgac gggatgtttt cgtctgacgg ctcggctgcc ccgctggctg aatacctgaa
     6301 gatcctgccc aaagatgcgc tgatcctcgt ggacgacgcc cacggcgccg gggtgctggg
     6361 ccgcaacggc caaggtgcgc ccgaacatgc gggtgtgagc cgccgccgcg tcatccaaac
     6421 cgtcacgttg agcaagtcgt tcggcgctta cggcggcgct atccttggca gcgcccggct
     6481 ccgccaacaa atcctggacc gcagccggat gttcgtcggc agcactcccc tgcccttgcc
     6541 actggccaac gcggcgctgg caggcgtcag aatcctgaaa acggacaaga gcttgaggca
     6601 gcggttggtt cagaacgcgg cttatatgaa agcggccttg cgcgaggcgg gcctgccgct
     6661 ggccgaaacg cccgggccga ttgtgcccct gcatccccgc cgcccgagtg aagccacccg
     6721 cttgaaccgc gctctgctgg cggcgggtgt ctatccaccg ttcatcaaat atcccggcgg
     6781 cccggcgagc ggctactttc gctttgtgat ttccagcgag cacagccgct cgcagctcga
     6841 tgccgtgctc agcgtgttgc tcgcttcttg aatgagcttt cagcaatgca gggggccgtt
     6901 cagttgacga atcgagctat ggctctgtgg cccataccgg gagagaaggc gccgcgcgcc
     6961 aagctgtgcg gtggccgccg gacaacttca accttgttcg gcctcatgct ttcgctcctg
     7021 gccagtcacg ccgcatcttc ttttcaggtg actgcgtttg ccctggacgg cctgttgacg
     7081 tggacaaatg cccccgtgcc cggaatctgc accgtcgagg cggccagcac actcgccggc
     7141 ccctggactc ctgggcagaa cgcctttgcc accaattcgg cggacacgct caccgtgccg
     7201 atcaccggag gaacccggtt ctatcgaatg cagggcgtcg cggttccacc cacaccacaa
     7261 gggtttacca acctggttta cgcctacggg atcctcgaaa ccatcgcggg caacggactt
     7321 ggccggctgg ataccagcaa ctactggtcc gcggcctatg aaggcgggcc agcaaccttg
     7381 gcggctttgt cccgcccgca catcgcgatg gccgaccggg ctggcaatgt ttatatcgcc
     7441 gacaagaaca gccactcggt cctccgggtc gcgccggatg gcactattca cactcacgcc
     7501 ggcacgcata ccggcggttt caacggtgaa gggccggccc ctagcacaac cctgcaactt
     7561 aacttcccta atggcctttg ggtccgctcg gatggcaccg tttatgtgct ggacacggat
     7621 aatgggcggg tgcggcgcgt gagcaccaac ggcatcatga cgacattgtt cctggccacc
     7681 agtgacggat cggccctcaa cacaggacgc ggcctctggg tcaaagacga tgaggcgctg
     7741 gcctatttct gtgccggaac aaaggtgcgg agttggacat ccacgaacaa tctgagcaca
     7801 ctcgcctcgg gcttctccga cctgggcgac ctgtttgtcg agccgggggg agatgtcatt
     7861 gtgtgtgatc gtggcgcgaa ttacgcctat cgcgtccatc ccaacggctc gtcggtcgtg
     7921 ctggccggca atggcaatac caatgggggc ggcgacggct tctcggcgct agcaaccggc
     7981 ctctatgggg tccgcagtcc ctggcccgtg cccacgggcg gctacctgct tctgtctcat
     8041 gaaggatgcc agctttggta tttagactct gccggcatta tccacctgtt gctgaacggc
     8101 tcaggcgggc tcacccacga cggcgacggc gccttcttct atgatccatc cgtcccaaaa
     8161 atcagtgaag gccgcagcgt cacgatggat tacgacgaca atgtgctgat ttgcgaaagc
     8221 gactacggtt acatccgacg tattcggttc cagagaatcc cacccgctag ctgattgagg
     8281 ctctgattcc tgaagcaatg ctgtagggtg cgacaaaaag ctccggcgcg aaacgtttgc
     8341 ttgattcagt ccattttgcc acactaccca catgcgcgcg ccactctgct gcctgctcgc
     8401 cctgtccgct tttgcccagg gcggcgaaag cccggccccc aatgccccct cccctgtcgt
     8461 ccatctcagc ttggtccgtc cggcgcacta ttccggcatg tgcgatgcct cgggcgcggt
     8521 tgcggtcagt tcaaatctct tcgttgtcgc gaatgacgag gacaatgtcc tgcgcctcta
     8581 tcgcagcgat cagccggggc aacctgtgaa gcagtttgat ttcaacgcct ttttggaagt
     8641 ccaaggcaag tcccttgaag cggatctgga gggggcagcc cggatcggcg atcgcgcctt
     8701 ctggatcggt tctcacgggc gcaataagga tggaaaggag cgtctcaacc gccaccgctt
     8761 gtttgcaacc gatatcagcg taaacgctgg agaggttgcc ctcaccgctg tcggcacacc
     8821 ttacagactg ctgctggacg atctgctccg ggatgcccga ttcgatcagt tccattttgc
     8881 ggaagccgcc catcgcgcgc ccaaagaccc cgacgctctg aacattgaag gcctttcggc
     8941 catggcggaa ggccaactgc tgattggctt ccgcaatccc gttccagcag gcaaagccct
     9001 gctcattccc ctgcttaacc cgaacgaggt catccaagcc aa
//
LOCUS       DATCGS010000343         4938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_375235, whole genome shotgun sequence.
ACCESSION   DATCGS010000343 DATCGS010000000
VERSION     DATCGS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375235"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VIL39_07030"
     CDS             complement(<1..800)
                     /locus_tag="VIL39_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEY5481215.1"
                     /translation="MNSAPALVVLAAGIGARYGGLKQMDPVGPGGEAVLDYAVYDAKR
                     AGFGKFIFVLRRQIEHDFREAFGRRFAKHLDIDYAFQELDMVPAGRHAPPDRTKPWGT
                     GHAVLCAAQVLDRPFAVINADDFYGAESFHVLARFLTQSAPANPDLFAMVGFQLDRTL
                     SEHGTVARGLCQTDAAGHLISVEELTAIERQPVGARNKERDGSYRNLTGRETVSMNCW
                     GFSMGLFDGLRRQFGEFLDRNEGNLKAEFYLPTAINALIQERRAQVKVL"
     gene            940..2112
                     /locus_tag="VIL39_07035"
     CDS             940..2112
                     /locus_tag="VIL39_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481216.1"
                     /translation="MAFKDFKGQEQGVELLQRSLERGRLAHGYLFTGHQLDQLEALAR
                     TLAKTLNCLHPVTRGGVAVDCCDQCLSCQKIEHGNHADVFWVRPESKLRQIRIRQIVR
                     RENSPPRVLLDAVNLKPTESQYKFAIIVAADRMNEEAANAFLKTLEEPPQHSVLILLT
                     TEPQRLLETILSRCLRLNFAAEGPQQLTPAQMEWLASFSETAAADQKSLLGRYRLMDV
                     LLKKLNEVKETIDETLTARSPLEKYEDVEEQLRDKWEQELKAAIEAEYRRQRGDFLLA
                     LQWWLRDVWVQTLNKVQSPKSEVQSPEAKVESTRPRIEAGGAGAETVNHQLSAIDSPE
                     GLLILPQMAGTKRVAQRISPKQAMENLRVMEQLQRWLSTNVQEALALEVGLLKLHL"
     gene            2147..3535
                     /locus_tag="VIL39_07040"
     CDS             2147..3535
                     /locus_tag="VIL39_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5481217.1"
                     /translation="MKSQPQPQGATNHELLPSIFAGLFGAFLGLALLKFGNPPITEKW
                     VSAPTNYWEFLFGYPWPIAWAYWLLIPVGVTGIATARWKSVAPWWLVGLPLLWLVWQF
                     IAGTRSVGPDLTALTLKHFTACILCFYLGYFSLSRVRRVWLFWVGLLFGFLLVLTVGW
                     EQQFGGLKESRHYFFTYVYPYMKDVAPEYLKKISSNRIFSTLFYPNALAGALLLLLPI
                     TLAALWRLRALLTPAARWFLIGVVGMAGLRCLYWSGSKGGWLLMLSVGLVGLLRVPFS
                     RQLKVALVIGILLAGLAGFFWKYAAFFQKGATSVSARFDYWRAAAQTTKDNPLFGTGP
                     GTFAIAYQKIKRPESEMSRLVHNDYLEQASDSGVPGLLAYTLFIVSMVVWSFPKAGKT
                     AAMGSDDWLVFSAWLGVLAWSLQSLVEFGLYIPALAWPAFTLLGWLLDRKEPKIPGTQ
                     VRLPAARRLEIH"
     gene            3573..4007
                     /gene="aroQ"
                     /locus_tag="VIL39_07045"
     CDS             3573..4007
                     /gene="aroQ"
                     /locus_tag="VIL39_07045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY5481218.1"
                     /translation="MRILFLNGPNLNLLGQREPEVYGHATLADIEAKVRARAKKLKAT
                     VDFRQSNLEGELVGWIQQARGKFDVIVLNAAAYTHTSVALRDAIAAVGIPTIEIHLSN
                     IHAREEFRHKSLIAPVCRGQIVGFGVNSYLLAVDASVNVNER"
     gene            4146..4613
                     /gene="accB"
                     /locus_tag="VIL39_07050"
     CDS             4146..4613
                     /gene="accB"
                     /locus_tag="VIL39_07050"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5481219.1"
                     /translation="MDLKDIKAIIDLMRKNSVTEFELEKDDFKIRLKRGTNGGATALA
                     SEVTQGLPNAPALTVAASGPAAAPPPPVPASNEVDIKSPMIGTFYRAPSPESAPYVEV
                     GVEVNPDTVVCIIEAMKVMNEIKAEAKGVITQAMVENAKPVEFGQPLFKIRPA"
     gene            4680..>4938
                     /locus_tag="VIL39_07055"
     CDS             4680..>4938
                     /locus_tag="VIL39_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5481220.1"
                     /translation="MFEKILVANRGEIAVRIIRACKELNIRTVAIYSEADANSMHVQM
                     ADEAICIGKAPSSDSYLRIDRIVSAAEITDVDAIHPGYGFLA"
BASE COUNT          992 a         1423 c         1521 g         1002 t
ORIGIN      
        1 agcaccttca cctgcgcccg tcgttcctgg atcagcgcgt tgattgccgt cggcaggtag
       61 aactccgcct taaggttccc ctcgttgcgg tcgagaaact caccaaactg gcggcgcagc
      121 ccatcgaaca gtcccattga aaacccccaa cagttcattg agacggtctc ccggccggtc
      181 aggttccggt agctgccgtc gcgctccttg ttccgggcac cgacaggctg cctttcaatg
      241 gccgtcaatt cttcgacaga tatcagatgc cccgcggcgt ccgtctggca gagcccgcgc
      301 gccacggtgc catgctccga caacgtccgg tccagttgga agcctaccat tgcgaacaga
      361 tccgggttgg cgggagccga ctgcgtcaga aaccgggcga gcacgtgaaa tgattccgcg
      421 ccgtagaagt cgtccgcgtt gatgaccgcg aaaggccggt cgagcacttg cgcggcgcac
      481 aatacggcat ggccggtgcc ccacggcttc gtgcggtcag gaggggcgtg gcgccccgcc
      541 ggcaccatgt caagttcctg gaaggcataa tctatgtcga ggtgtttggc gaaacggcgg
      601 ccgaaggctt cgcgaaagtc gtgctctatc tgcctgcgga ggacgaagat gaacttgcca
      661 aagcccgccc gcttcgcgtc atagaccgcg tagtccagga ccgcctcgcc gcccggtccg
      721 acggggtcca tctgtttcaa tccgccgtag cgggcgccga tgccggccgc caaaacaact
      781 aatgcaggcg ctgagttcaa gagtatgcgg catacgctac ggaacccgag ggaaggtgca
      841 agcgcaaaac tcccccgggc gtcgttgcgc taatctccgt tcagaagatt gtgaaatgac
      901 ttcttccctt ctttgggccg gggaattata agagtcgcga tggcattcaa agactttaaa
      961 gggcaggagc agggcgtgga gttgctgcag cgctcgctgg aacgcgggcg cctggcccac
     1021 ggctacctgt tcaccggcca ccagctcgac caactggaag cgctggcccg caccctggcc
     1081 aagacgctca actgccttca cccggtcacg cggggcggcg tggccgttga ctgctgcgat
     1141 caatgcctca gttgtcagaa gatcgagcac ggcaatcacg cggatgtgtt ctgggtgcgg
     1201 ccggagtcga agctgcgcca gatcaggatc cgccagatcg tgcggcggga gaatagcccg
     1261 ccgcgggtgc tcctggacgc cgtcaatctc aagcccactg agagccaata caagtttgcc
     1321 atcatcgtcg ctgccgaccg gatgaacgag gaggctgcca atgcctttct caagacgctg
     1381 gaagagccgc cgcagcattc cgtcctgatt ctcctgacga ccgagccgca gcggctgctc
     1441 gaaacgattc tctcacgttg cctgcggttg aacttcgccg ctgaggggcc gcagcagttg
     1501 acgccggcgc aaatggagtg gctggcgtcg ttcagcgaga cggcggcggc ggaccagaag
     1561 agtctgctgg ggcgctatcg tctcatggac gtactgctga agaagctgaa tgaagtgaag
     1621 gagaccattg acgagaccct gaccgcgcgt tcacctctgg agaagtacga agacgtggaa
     1681 gagcaactgc gggacaaatg ggagcaggaa ctgaaagcgg ccattgaagc ggagtatcgc
     1741 cggcagcggg gcgatttcct gctggcgcta caatggtggc tgcgggatgt gtgggtgcag
     1801 acgcttaaca aagtccaaag tccaaagtct gaagtccaaa gtccggaagc caaagtcgag
     1861 agcacaaggc ccaggatcga agcaggcggt gcgggggcgg agactgtcaa ccatcaacta
     1921 tcggccatcg actcccccga gggtttgttg attcttccgc aaatggctgg gacgaagcgc
     1981 gtggcccaga ggatctcgcc caagcaggcc atggagaatc tgcgcgtcat ggagcagctc
     2041 cagcgttggc tgagcacaaa tgtccaggaa gcgctagccc tggaagtggg gctgctcaag
     2101 ctgcatttgt agcgccagcc ccattgggag aaagcccgcc cggcccatga agtcccaacc
     2161 gcaaccacag ggagcgacaa accatgagtt gctgccgtcc atttttgccg ggctgtttgg
     2221 cgcattcctg ggattggcgc tgctgaaatt cggcaacccg ccgatcaccg agaaatgggt
     2281 cagcgctccc acgaattact gggagttcct gttcggttac ccctggccga ttgcctgggc
     2341 gtattggctg ttgattccag ttggagtaac cgggatagcg actgcgcgat ggaagtcggt
     2401 tgcgccctgg tggctcgtcg ggctgcccct gctctggctg gtttggcagt tcattgccgg
     2461 cacgcggtcg gttggccccg acctcaccgc gctcaccctc aagcatttca ccgcgtgcat
     2521 cctgtgcttt tacctcgggt atttctcgct gagccgggtg cggcgggtct ggcttttttg
     2581 ggtgggcctg ctgtttgggt tcctgctagt gctgaccgtc ggctgggagc agcagttcgg
     2641 cggcttgaag gagtcgcgcc attatttttt cacgtacgtc tatccctaca tgaaggacgt
     2701 ggcgccggag tatctgaaga agatctccag caaccgcatc ttctcgacgc tcttttaccc
     2761 gaatgcactg gccggggcgc tgctgctgct gctgccgatc accttggcgg cgctgtggcg
     2821 actgcgcgcg ctcctcacgc cggccgcccg gtggttcctg atcggggtgg tcgggatggc
     2881 tgggttgcgt tgcctctatt ggtccgggtc gaagggcggg tggctgttga tgctgtcggt
     2941 cggcctggtt gggctgttga gggtgccctt cagccgccaa ctcaaggtcg ccctggtgat
     3001 cgggatcttg cttgccgggt tggctgggtt cttctggaag tatgccgcat ttttccagaa
     3061 gggggccacc agcgtcagcg cccggttcga ttactggcgg gcggcggccc agacgactaa
     3121 agacaacccg ctcttcggca cggggccggg gacgttcgcc atcgcctatc agaagatcaa
     3181 acggcctgaa tccgagatgt cgcgcctggt gcacaacgac tacctggaac aggcgtccga
     3241 ctcaggagtg ccggggctgc tggcctacac cctgttcatt gtcagtatgg tggtgtggag
     3301 cttccccaag gcaggaaaga ctgcggccat ggggtcagac gattggctgg tcttctcggc
     3361 ctggcttggg gtgctggcgt ggagtttgca gagtctggtt gagtttgggc tctatattcc
     3421 agccctggct tggccggcct tcaccttgct cggctggctt ttggaccgta aggaaccgaa
     3481 gattcccggg acccaggtgc ggttaccagc cgccaggcgc ttggaaatcc attagacaaa
     3541 gtcggccaaa gtcgttagct tttcctcacg gaatgagaat cctgttcttg aacggtccga
     3601 atctgaacct gctcggccag cgcgagccgg aagtgtatgg ccacgccact ttggcggata
     3661 ttgaggccaa agtccgcgcc cgggccaaga aactgaaggc aaccgtggat ttccgccagt
     3721 ccaacctgga gggggagttg gtcggctgga tccaacaggc gcgggggaag ttcgacgtga
     3781 tcgtcctgaa cgcagccgcc tacacccata cgagcgtcgc cctgcgggac gcgattgcag
     3841 cggtcgggat accgacaatc gaaatccacc tctcaaacat ccatgcccgg gaagaattcc
     3901 gccataagtc gctgatcgcg ccggtatgcc gaggccagat agtcgggttt ggcgtaaatt
     3961 cttatctttt ggcagtggat gcatcggtta acgttaacga aaggtgaata atggcctcaa
     4021 ctagttgtat tttgtgttct acttattaag ttcgtatgcc cgaaataagc caaagatttg
     4081 cctgttgact ccctccgaca ggttgattat tctttcccgc gatcggacac taacaacaaa
     4141 ccgctgtgga cttaaaagac atcaaagcca ttatcgacct gatgagaaag aactccgtaa
     4201 cggagttcga actcgagaag gatgacttca agatcaggct aaagcgggga acaaatgggg
     4261 gcgcgactgc actcgcctcc gaggttaccc agggtctgcc gaatgccccg gcactgacag
     4321 tagctgcgtc tggcccggct gccgcacccc cgcccccagt gcccgccagc aacgaagttg
     4381 atatcaaatc tcccatgatt ggcacgttct accgggcacc ctctcccgaa tcagctcctt
     4441 atgtcgaagt gggcgtcgaa gtcaatcccg acacggtcgt ctgcattatc gaagcaatga
     4501 aggtgatgaa cgagatcaaa gcggaggcca agggagtcat cacccaggca atggtcgaga
     4561 acgccaaacc ggtggagttc ggccagccgt tgttcaagat ccgcccggcc tgacttggca
     4621 tgaagtatgt catgccaaga ggatcggtct aacgctctaa cgtttcaacg tcattaggca
     4681 tgtttgagaa gattctggtg gcgaatcgcg gggaaattgc cgtacgcatc atccgcgcgt
     4741 gcaaggagct taacatccgc acggtggcga tctattcgga agcggatgcc aactcgatgc
     4801 acgttcaaat ggcggacgaa gcgatctgca tcggcaaagc accctccagc gatagctatc
     4861 tgcggattga ccgcattgtc agcgcagcag agatcactga tgttgacgcc attcaccccg
     4921 gctacgggtt cctggccg
//
LOCUS       DATCGS010000344         4678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_723059, whole genome shotgun sequence.
ACCESSION   DATCGS010000344 DATCGS010000000
VERSION     DATCGS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723059"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VIL39_07060"
     CDS             <1..271
                     /locus_tag="VIL39_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEY5481221.1"
                     /translation="EVMRLKHYSIRTERCYCDWIRRYIHFHKMRSRDDLQGAEPKIEL
                     FLSDLAVNGHVAASTQNQAFNALLFLYREMKKLNVDLHDVSAGRV"
     gene            320..1819
                     /locus_tag="VIL39_07065"
     CDS             320..1819
                     /locus_tag="VIL39_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY5481222.1"
                     /translation="MGKATDIRTVGAALYFLPVQTRVALKFGAETLTHVTCARARVTV
                     SDDQGRTAEGWGETPLSVQWVWPSATAYEERHAAVKTFCIELTELWASVRSCGHPIEL
                     GHDFLETVLPGWLQGFNQRHRRGKEPMPWLAALVCCSPFDLAVHDAYGQLHGCPTYET
                     YHAGFMNRDLAYYLQPAPRSGVTFQGKYPADFLAAHRAESLVAWHLVGGLDLLEEAEL
                     TGTEPNDGYPVLLEDWIRRDGLKCLKVKLRGNDADWDFARLVKVGAIGLKHGVDWLSA
                     DFNCTVSDPEYVNAILDRLRDEHPRVYGMILYVEQPFPYELEQYRMDVHSVSARKPLF
                     LDESAHDWRLVRLGRELGWSGVALKTCKTQTGALLSACWARAHGMSLMVQDLTNPMLA
                     QIPHVLLAAHAGTIMGVETNAMQFYPEASRPEAEAHPGLYQRRKGKVELCTLRGPGFG
                     YQLREIKRELPVPAAECSGWNRPPLGPKELERRAETAQEPHSCDEGVLR"
     gene            1871..2197
                     /locus_tag="VIL39_07070"
     CDS             1871..2197
                     /locus_tag="VIL39_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481223.1"
                     /translation="MNDPLRMLHLEDDPDYCDLVRALLAQEGYRVESVLADDRASFQA
                     ALAPEKFDVILADYSLPSYNGLEALRLAREKCPDTPFLLVSGTIGEEAAIESLKCGAT
                     DYVLKQ"
     gene            2219..>4678
                     /locus_tag="VIL39_07075"
     CDS             2219..>4678
                     /locus_tag="VIL39_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5481224.1"
                     /translation="MRRAIKEAAERKQRRQAETELSRREKYFRALTENALNILSIVDR
                     EGVFTYHSPSVKNVLGYEPEELVGRSAFDSIHPEDLPGVLQGFDNGLKHPDRTFTLEF
                     RVPKRDGSWRCLEAVCQSRLGDPEIAGVAVNSRDVSDRKLVEAGLRESEKRYRLIFDG
                     NPTPMLVFDHETLVFLEVNEAAVQHYGYSRQEFRRMTLKDIRPPDDGPAMIEYVHEFV
                     VGSASSGVGFAGAWRHRRKDGSVMDVEIKWSPISFGGWAASLAMVNDITQRKRIEHRN
                     AALSKLGQNLSSATSPAEAAGIIRAVADELFEWDVFTLDLYFAELGSISPILNVDTAR
                     NGQRFDIPVAGKLTKPSGMARRIVAQGAELILRIEPIHMPEDVFPIGDTSRPSASLML
                     VPIRNRTKVIGILSIQSYRTKAYDQADLGTLQTLADHCGGALERIRAEQALRDSEQRF
                     RELFEGSPDAVFVKDLTGTVLDINPAACRLHDAPRQNLIGTNVLDLVPSAQRAEVERE
                     FNALVEGRLQQIEGISCTLDGRAIPVEIRSSRINYAGKPAVLLHVRDITDRKLAEAAL
                     RSSEMLFHSVWENSADGMRLTDEQGIMVAVNDAFCKLVGMQREDLEGKAFTVVFADSE
                     QPEKMLQEFRQRFRDRVIEQPRELRLSLRNGNVVTLEDTSSFVELRGQLPMMLGLFRD
                     VTDQKRLEEQLRQAQKMEAIGQLAGGVAHDFNNILTVILGHASLLQAAGNLTGVAARS
                     AQQISQAAERAATLTRQLLAFSRRQLMQLRQLDMNEVVANMTKMLGRILGEDIALQLN
                     YSPQPARVHADAGMMEQVLLNL"
BASE COUNT          912 a         1289 c         1592 g          885 t
ORIGIN      
        1 ggaggtaatg aggctgaaac attactccat ccgcaccgag cgctgctact gcgattggat
       61 tcggcgctac atccatttcc acaaaatgcg ttcccgggac gacctgcagg gagcggagcc
      121 gaagatcgaa ctgtttttaa gcgatctagc cgtcaatggc cacgtggccg cctcgacgca
      181 gaatcaggcg tttaacgcgc tgctgttcct ctaccgggag atgaagaagc tcaacgtaga
      241 tttgcacgac gtgagcgccg gtagagtata gctaaatcgc ccttgttgac gacggcggct
      301 tgaggcttaa tcaaaggcga tgggcaaggc aacagacata cggacggtcg gggcggcgct
      361 ttacttcctg ccggtgcaga cacgggtggc gctgaagttc ggcgcggaga cgctgaccca
      421 cgtgacgtgc gcgcgggcgc gcgtgacggt gagcgatgac caggggcgga ccgcggaggg
      481 gtggggggag acgccgctga gtgttcagtg ggtctggccg agtgccacgg cttacgagga
      541 gcggcacgcg gcggtaaaga cgttttgcat tgagctgacg gagttgtggg cgagcgttcg
      601 ttcgtgcggt catccgattg aactggggca tgattttctg gagactgtgt tgccgggctg
      661 gctccagggg ttcaaccagc ggcatcgccg gggcaaggaa ccgatgccgt ggctggcggc
      721 gctggtgtgc tgttcgccgt tcgacctggc ggtgcatgat gcctacggcc agttgcatgg
      781 ctgcccaact tacgagactt accacgccgg gttcatgaac cgcgacctgg cttactactt
      841 gcagccggcg ccgcgatcag gagttacctt tcaaggcaag tatccggctg acttcctggc
      901 cgctcaccgc gcggagagcc tggtggcctg gcatttggtg ggtggattgg acttgctgga
      961 ggaagcggaa ctgacgggca cggagccgaa cgacggatac ccggtgctgc tggaggattg
     1021 gattcggcgc gacggcctga aatgcctaaa ggtgaagctg cgcggcaatg atgcggactg
     1081 ggatttcgct cgcctggtga aggtgggcgc gattggcctc aagcacggcg ttgattggct
     1141 gtcggctgat ttcaattgca cggttagtga tccggagtat gtgaatgcga tactcgaccg
     1201 cctgcgggat gagcaccctc gggtttacgg catgattctc tatgtggagc agccgtttcc
     1261 ttacgaactg gagcagtatc ggatggacgt gcacagcgtg tcggcccgca agccgctgtt
     1321 cctggacgaa agcgcgcatg actggcgcct ggtgaggttg gggcgggagt tgggctggtc
     1381 gggcgtggcg ctcaagacgt gcaagacgca gaccggcgct ttgctgagcg cctgctgggc
     1441 cagagcccac ggcatgagcc tgatggtgca ggacctgacg aatccgatgc tggcgcagat
     1501 cccgcacgtg ctgctggccg cgcacgcggg gacgatcatg ggcgtggaaa ccaatgccat
     1561 gcagttctat cccgaggcct cgcgcccgga ggccgaggcg catcccggcc tataccaaag
     1621 gcggaagggg aaagtcgagc tttgcacctt gcgcgggccg ggcttcggtt atcagttgcg
     1681 agagatcaaa cgggagctgc cggtgccggc agcggagtgt tccggctgga acagaccgcc
     1741 tttaggtcca aaagagctgg aaagaagggc ggaaaccgcc caggagcccc attcctgcga
     1801 tgagggtgtc ctgcggtaat ggcaccaagg ttgctgctaa agaaccagaa tcacgcagga
     1861 ttactgtgcc atgaacgacc cgttgcggat gttgcatctt gaagatgacc cggattattg
     1921 cgatctggtt cgagctctgt tggcccagga aggctaccgg gttgagagcg tgctggcgga
     1981 tgaccgcgcg agttttcaag ccgctttggc tcccgagaag ttcgatgtca tcttggcgga
     2041 ttattcgctc ccctcctaca atggcctcga ggcgctgcgg ctggcgcgtg agaaatgccc
     2101 cgatacacct tttctgctcg tctccggcac gattggagag gaggcggcca tcgagagtct
     2161 taaatgcggg gcgaccgatt atgtgctcaa gcagtgaccg gatcgactgg ttccggcggt
     2221 gcgccgcgcg atcaaggagg ctgcggagcg gaagcagcgg cggcaagcgg aaacggaatt
     2281 gagtcggcgc gagaaatatt tccgcgccct gacggagaac gcgctgaaca tcctcagcat
     2341 cgtggaccgc gagggggttt ttacctacca tagcccctcc gtaaagaacg tgctggggta
     2401 tgagcccgag gagctggtgg gacgaagtgc ctttgactcc atccatcccg aagacctccc
     2461 cggcgttttg caagggttcg acaatggcct gaagcatccc gaccgcacgt tcacgctgga
     2521 attccgtgtc ccgaagcggg acggctcctg gcgctgcctg gaggcggtct gccagagccg
     2581 cctgggcgat ccggaaatcg ctggggtggc cgttaactcg cgcgacgtca gtgatcgcaa
     2641 gctggtggaa gcggggttgc gcgagagtga aaagcggtat cgcctgattt tcgacggcaa
     2701 ccccaccccg atgttggtct tcgatcatga gacgctcgtc ttcctggaag tcaatgaggc
     2761 cgcggtgcag cattacggct attcgcggca ggagtttcgg aggatgacac tgaaggacat
     2821 ccggccacca gacgatggtc cggcgatgat cgagtatgtg cacgagtttg tggtggggag
     2881 cgcatccagc ggagtcggat tcgccggcgc gtggcggcac cgccgcaagg acggatcggt
     2941 catggatgtc gaaatcaagt ggagcccgat ctccttcgga ggctgggcgg cgtcgttggc
     3001 gatggttaac gacatcaccc agcgaaagcg gattgagcat cggaacgcgg ccctttccaa
     3061 gctgggccag aatctcagct cggcaacttc gcccgccgag gcggccggga ttatccgcgc
     3121 cgtcgcggat gagttgtttg agtgggatgt gttcacgttg gacctgtatt tcgctgaact
     3181 gggcagcatt tccccaatcc tgaacgtgga caccgccaga aatggccagc ggttcgacat
     3241 tccggtggcg ggcaagctaa caaaacctag tggcatggcg cggcggatag tggcacaagg
     3301 cgcggagctg atcttgcgga tagagccgat ccacatgccc gaggacgtgt ttccgatcgg
     3361 ggacacctcg cgaccctcgg cttcgctgat gctggtgccg atccggaatc ggaccaaggt
     3421 gattggcatc ctgtcgatcc aaagctaccg aaccaaggct tatgatcagg cggatctcgg
     3481 cacgctgcaa acgctggccg accactgcgg cggggcgttg gaacgtatcc gggcggagca
     3541 ggcgctgcga gacagcgagc agcgtttccg cgagttattt gaaggttcgc ccgatgccgt
     3601 ctttgtgaag gacttaaccg gcaccgtgct ggatattaat ccggcggcct gccggctgca
     3661 tgacgcgccg cgccagaatc tgatcggaac gaacgttctg gacctggtcc catccgcaca
     3721 gcgggcggag gtggagcgcg agttcaacgc gctcgtggaa ggccggctcc agcaaatcga
     3781 agggatcagt tgcacgctgg acggccgggc catccccgtg gaaatccggt ccagccgcat
     3841 caattacgcg ggcaagccgg cggtgctttt gcacgtgcgt gatattaccg accgcaagct
     3901 ggcggaggcg gcgctacgaa gttcggagat gctgttccat tcggtatggg aaaactcggc
     3961 ggatgggatg cgcctgaccg acgagcaggg gatcatggtg gcagtcaacg atgccttctg
     4021 caaactggtc ggcatgcagc gggaagatct ggaagggaag gctttcaccg tcgtcttcgc
     4081 cgattccgag cagccggaga agatgctcca ggaattccgc cagcgcttcc gggatcgggt
     4141 catcgagcag ccgagggagc tccgtctgag cttgcgcaac ggcaatgtcg tgacgctgga
     4201 ggacaccagc tcgtttgtgg agttgcgggg ccagctcccg atgatgctgg ggctgttccg
     4261 ggacgtgacg gaccagaagc ggctcgaaga gcagttgcgc caagcccaaa agatggaggc
     4321 gatcggccag ctcgccggcg gggtggccca tgatttcaac aacattctga cggtcatcct
     4381 cggtcatgcc tcgctgctcc aggccgctgg caacctgacc ggagtggccg cccgatccgc
     4441 ccagcaaatc agccaggccg ccgagcgggc cgccaccctg acgcgacaat tgctggcctt
     4501 cagccgccgc cagttgatgc aactgcggca gttggacatg aacgaggtgg tcgccaacat
     4561 gaccaagatg ctggggcgca ttctcgggga agacatcgcc ctgcaattga attactcccc
     4621 gcagccagcg cgagtgcatg ccgacgccgg catgatggaa caggtattgt tgaacctg
//
LOCUS       DATCGS010000345         4231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_525649, whole genome shotgun sequence.
ACCESSION   DATCGS010000345 DATCGS010000000
VERSION     DATCGS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525649"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2292)
                     /locus_tag="VIL39_07080"
     CDS             complement(127..2292)
                     /locus_tag="VIL39_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5481225.1"
                     /translation="MNLFNSPLSARAPRWKTEFWSLAVVLLLVVLAPRAGAEKAAAVP
                     DFTFIQASDVHAPMAQSKATIAKIPGLGEIDLAPFGVKVPKPGFVIVTGDMNEFGGGS
                     GWWAEYLSYWKGCSVPVYHGLGNHDNTWHANLKGLRDLGLGPCYSFDKSGCHFVSLMT
                     PTLQDPRPSVGEEELIWLKKDLAKVGTRTPVFVFCHHPLEGTEFASRYDYDRLLDVLH
                     RYNTVLLMAGHSHGHVHHVVEGIDQTTGGSTFGPLAGFAVISVKDGMLREAYWKADQP
                     APDLKLVEKPIPASSSYPKVEIRSPAFRASGGDTLSISAKLAGPVALEKAAYAIDDEM
                     TGELKLSGQAPAWTATGSADLSKLLPGAHCLRVDFTRGKEHYSRSTEFFFEPANQPTA
                     WRAYLAASSKSTPAIADGVVYVGANDGNLRAFNSRTGKQLWAADTGAEILAEPLVDGG
                     KVYSANGLGVVSAFSKKGKQVWSFTAGDAVYSSPVMADGKVIFGCNDGWLYAVDAATG
                     KLAWVNKDGTYTIESKPFAWNGKVYFGAWDEYIRCVNIQDGKLVWQHICEGSRVAKAA
                     KRYYSPADAMPVVAEGKLMIADRNMMLTILNAETGEPSGSMKGVSATGLSEDGKFVYL
                     RKMNGSVAKIDSTGKEVWSVGAHVGAIPTAPTEKNGVVYVSSGKGTVSAITADSGKVL
                     WEYQGSPQLFVMCSVACDGENAYLTAFDGSLTAIKCRAR"
     gene            2769..>4231
                     /locus_tag="VIL39_07085"
     CDS             2769..>4231
                     /locus_tag="VIL39_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY5481226.1"
                     /translation="MKTGSLWLLLAGLVLTGSAWGQTPTVKTDREVLMGPLSKDEAPA
                     WLAAMRHWREERLGKMRYSGAEYDRPELKWTQRSFIQPQMLVEDRYFYDPVAGKYTVD
                     RYLNDLEKRYGGIDSVLVWAVYCNIGIDWRNQHDMVRALPGGLPGVRQMVADFHRRGV
                     RVLFPVMPWDVGTRDEGAPLWTAIARTLKEVGADGVNGDTITGIPKEYRTASDESGQS
                     LVFEPECGLNGEAELPWNNMSWGYWAYHAIPTVSHYKWLEPRHMVHVCNRWARDHTDD
                     LQHAFFNGTGFESWENVWGIWNGLTPRDAEAVRRIATVEHACSGLIASYGWEPHYHTL
                     QKGVFASKFPGVRRTLWTLVNRTEYDIPGPQLRVPHETGRRYFDLWHGVELTPTFTTN
                     QGGRSANLSFEIEGRGYGAVLAWNNESLDADFQSLLVSMKGMSGKPLRAFSSEWKFLP
                     QTQVPIKAVRAPRNPPSDMVRIPGGGFEFVVSGIMVEG"
BASE COUNT          764 a         1346 c         1300 g          821 t
ORIGIN      
        1 ccggtgatgg aacccttaca gggttctggt tccttgcggt actccactca gggtagctcg
       61 cggcctcgca accctgggct atgtgatgca atcctttcag gattgagctg cggggagaat
      121 ctgcgcctac cgcgcccggc acttgatcgc cgtcaggctg ccgtcgaacg ctgtgaggta
      181 ggcgttctct ccgtcgcagg ctaccgagca catcacgaag agctgaggtg atccctggta
      241 ttcccagagg accttgccgc tatcggcggt gatggcggat acggtgccct tgccgctcga
      301 cacatagacc acaccgttct tctcggtcgg cgcggttggt atggccccga cgtgggcgcc
      361 cacgctccag acctccttcc ctgtgctatc gatcttggcg acgctgccat tcatcttcct
      421 cagataaacg aacttgccgt cctcggacag gccggtggcg gagacgccct tcatcgagcc
      481 ggaaggctcg ccggtctcgg cgttgaggat ggtcagcatc atattgcggt cggcaatcat
      541 cagcttgccc tcggcgacca ccggcatggc gtcagccggg ctgtagtagc gtttggcagc
      601 cttggcgacc cgcgagccct cgcaaatgtg ctgccacacc agcttgccat cctggatgtt
      661 cacgcagcgg atatactcgt cccaagcccc gaaatagacc ttgccattcc atgcgaatgg
      721 cttggactca atggtatagg tcccatcctt gttgacccag gccagcttgc cggtggccgc
      781 atccacggcg tagagccagc catcgttgca gccgaagatc actttgccgt ccgccatcac
      841 cggcgaggaa tagaccgcgt cgcccgccgt gaacgaccac acctgcttgc ctttcttgct
      901 gaaggccgag acgactccca ggccattggc gctatacacc ttcccgccgt ccaccagcgg
      961 ctcggccaat atctccgcgc ccgtgtcggc cgcccacaac tgcttaccgg tccgggaatt
     1021 gaacgcgcgc aagttgccgt cgttcgcgcc cacatacacg accccgtcag cgatggcagg
     1081 cgtgctcttg ctcgacgccg ccagataggc gcgccaggcc gtgggctggt tagcgggctc
     1141 gaagaagaac tccgtgctcc gcgagtaatg ctctttgccc ctggtaaaat caacgcgcag
     1201 gcagtgcgct ccggggagca gtttgctcaa gtccgcgctg ccggtcgccg tccaagccgg
     1261 tgcctggcct gaaagcttca gctcgcccgt catctcatca tcaatcgcat aggcggcctt
     1321 ctccaaggcc actggacccg ccaacttggc cgagatgctc agcgtgtccc cgcccgacgc
     1381 gcggaaggcc ggggagcgga tctcaacctt cggatacgaa gagcttgctg ggataggctt
     1441 ctcgacgagc ttcaggtccg gagcgggttg gtcagccttc cagtaggcct cgcgcagcat
     1501 gccgtctttg accgaaatga cggcaaaccc cgcgagcggt ccgaaggtcg agccgccggt
     1561 ggtctggtca atgccctcca cgacgtggtg cacgtgtcca tgcgaatgcc cggccatcag
     1621 cagcaccgtg ttatagcgat gcagcacgtc caacagccgg tcatagtcgt aacggctcgc
     1681 aaattcagtc ccttccagcg gatggtggca gaacacgaac accggagtcc gggttcccac
     1741 ctttgcgagg tccttcttga gccaaataag ctcttcctcg ccgacggacg gccgcgggtc
     1801 ctggagggtg ggcgtcatca gactcacaaa gtggcagccg gacttgtcaa acgagtagca
     1861 agggcccagc ccgaggtcgc gcaggccctt caggttggcg tgccacgtat tatcgtggtt
     1921 ccccagcccg tgatacaccg gcacgctgca ccccttccaa tacgacaggt attcggccca
     1981 ccatccgctg ccgccgccga actcattcat gtcgccggtc acaatcacga agcccggctt
     2041 gggaaccttg acgccgaacg gcgcgaggtc aatctccccc aggcccggga tcttcgcgat
     2101 ggtcgccttg ctctgggcca tcggcgcatg cacatcactc gcctggatga aggtgaagtc
     2161 gggtaccgcc gcggccttct ccgccccggc acggggcgcc aggacgacca gcagcagcac
     2221 caccgccagt gaccagaact ctgttttcca gcgcggcgcg cgcgctgaca gtgggctgtt
     2281 gaataaattc accggcgcat cctgaagtct atgccggagc tcgtcaaggc aacgagcgtt
     2341 aaggttcccg cgctcgcctg cctctggcgg cctaactggc cgcttaatgg ctcatccggg
     2401 aaagtttgtg cctgcgatgc tgtactgtgc cctgttgatc aaccaggccg ggacgttagt
     2461 gtccgttccc tccttccgac cgtgccccac tccttcagcc tcggcactgc cagccaccac
     2521 tcggcctgct accggcgtgt aagcagcgcg tgagtagggt gcaagcagcg tgttgttcgc
     2581 tagggtttcg ctaggtcttc gctaggtctt cgctagggtt tgcggggttt ttgccgcctc
     2641 cccacctccc cagtcccacg gtgcatcccg cactctctga agacggactg acgcccatct
     2701 ctgagccggc tcccgattgc aggagcgtat tgcgcaacgg cgtgcggttg ctacattggc
     2761 agctcacgat gaaaacaggt tctttatggc tgttgctggc gggtttggtg ctgacaggtt
     2821 ccgcctgggg ccagacgccg accgtcaaaa cggaccgcga ggttcttatg gggccgctct
     2881 ccaaagatga ggcgcccgcg tggctcgccg ccatgcgcca ctggcgcgaa gagcgtcttg
     2941 gcaaaatgcg ttactcgggc gccgagtatg atcggcctga actcaagtgg acgcagcgca
     3001 gtttcattca gccgcagatg ctggtggagg accgctattt ctacgatccc gtggccggca
     3061 aatacacggt ggatcgttac ctgaatgatt tggagaagcg ctatggcggc atagatagcg
     3121 tgctggtgtg ggcagtgtat tgcaacatcg gcattgattg gcgcaaccag cacgacatgg
     3181 tgcgggcgct gccgggcggc ttgcctggtg tccggcagat ggtggcggat ttccaccgcc
     3241 gcggagtgag ggtgctgttc ccggtcatgc cgtgggacgt ggggacccgc gatgaaggcg
     3301 cgccgctttg gacggcgatt gcccgcacgc tcaaggaagt gggcgccgac ggcgtgaatg
     3361 gggacacgat cacgggcata ccaaaggagt atcgcacggc atcggacgag agcggccaga
     3421 gcctcgtctt cgagccggag tgcgggctca acggggaggc ggagttgccc tggaataaca
     3481 tgagctgggg ctactgggcg taccatgcca tacccacggt cagccactac aagtggctcg
     3541 aaccgcgcca catggttcac gtgtgcaatc gttgggcgcg cgatcacacc gatgatctcc
     3601 agcacgcgtt cttcaacggc acgggctttg aaagctggga gaatgtctgg ggcatttgga
     3661 acggtctgac cccgcgcgat gcggaggccg ttcgtcgcat tgccacggtc gagcatgcct
     3721 gttcgggact gatcgccagt tatgggtggg agccgcatta ccacacgctt cagaagggtg
     3781 tgtttgccag caagttccca ggcgtgcgcc ggacgctttg gacactcgtc aaccgcacgg
     3841 aatacgacat ccccggcccg caactgcgcg tgccgcacga aaccgggcgg cgctatttcg
     3901 atctgtggca tggcgtcgag ttgacgccca ccttcaccac caaccagggc ggccggagcg
     3961 ccaacctgag cttcgagatc gaaggccgcg gttacggtgc cgtactggcg tggaacaacg
     4021 aatcgctcga cgcggacttt cagtcgctgc tggtcagcat gaaagggatg tccggaaagc
     4081 cgctccgcgc cttctccagc gaatggaaat tcctaccgca aacgcaggta ccgatcaagg
     4141 cagtcagggc tcccaggaac ccgccgagcg acatggtccg cattcctggc ggcgggttcg
     4201 agttcgtggt ctccggcatc atggtggaag g
//
LOCUS       DATCGS010000346         5809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_129768, whole genome shotgun sequence.
ACCESSION   DATCGS010000346 DATCGS010000000
VERSION     DATCGS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129768"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VIL39_07090"
     CDS             complement(<1..1238)
                     /locus_tag="VIL39_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481227.1"
                     /translation="MEIVPNQPQAPAFKRLGGRLFHAGLKVQSLTEVFEVDRKTLQRW
                     VRALQSGDAQERVRVWAGRSAGRKLTPTIEAFVRQRWPRLTAQGTYGSSQRLRQEIHE
                     IFGVDLSPETLRPLVQELKAGTGPVESEDDPEASGGTARSASPVADGLRASALAAEKR
                     ETPGDCEGGEPEGDCPSNSLVINTHSLDDLQTHNPKESPVLLGSTPGQSVWCEHAGVL
                     LFARALNQIRAVVEPPQPLLRQWSATLLLGAMNIEQTKFLNGQDLELLLGTVVRFPAP
                     QRDLLKSVSTEATVAALFRFNAQGLSPGSDLYFDPHTEHYTGQQNVLKGWCPGIRLAD
                     KALHSDFIHTHRGEPIYFETTDNFEDLRQRFFAVLTRCRKVLNWPAERVLTTVVDRGI
                     FGAEVFEKVLADPALHLITW"
     gene            1552..2232
                     /locus_tag="VIL39_07095"
     CDS             1552..2232
                     /locus_tag="VIL39_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481228.1"
                     /translation="MNKLITPKIGPQMMGDAYTVSDLLAALSTEQFNKLIGFARLRLR
                     AVTRSLELRGCLASTDPEDLVAEAFLKLKLGEDNPSLGRHLKARNRTSTEAFLESVRG
                     IINSDLDHLVQEAKARYLHLPVGDVDNEPGAVDPAAPEDTYQLLSRRDLQQVLFAKLY
                     QSIRNQPALLGIVRDWEERFLDDDHIGVEGQNASMVHRVRQFAREILAELAAELSPSP
                     GDGKELFL"
     gene            2229..2615
                     /locus_tag="VIL39_07100"
     CDS             2229..2615
                     /locus_tag="VIL39_07100"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HEY5481229.1"
                     /translation="MIADDFWGTPIFRYTRADALRDGVLLDISETASVCARLQVPAAM
                     TVGLWDVLAPGEPFDVQDERLKVVLYGLLLAAMGIMPRRIFDTPSAHIMVYVLEVESK
                     VVRVKAAAHFDETDQPVCTLMLESED"
     gene            complement(2612..4474)
                     /locus_tag="VIL39_07105"
     CDS             complement(2612..4474)
                     /locus_tag="VIL39_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEY5481230.1"
                     /translation="MKPTNTPLVVWPCYEPAGQTIALTEEDLVQHVLVVGSTGCGKTT
                     LLRGAMDQLISHESRIGLLILDAKQDGTVEHVIEVAGRAGRERDLVVLGPQGTHILDL
                     FGPLKTLGDVDLVTQRLLLATEPVGRDNPYWQTTTAAMIAAALTLLLVRKRPISFGDA
                     IEFMRSWFVGLESFATLPKPVADVVEQGKREAKKPGSAPQLLGALDHVEVWKHLDPRT
                     RSNLQSCLLNVLRPLLSAAAVRYLDANDRPAFSPALVAEGKLCVVSINALIHPELAAF
                     VLRLARREFFDAVQTRGTGEHPLCGLIADEFPLIVRPEDADQLATLRSKRCFVLAATQ
                     GLAAIDDKIGFRLRRAVLLNYNTLVFMRTREEEAGELAAMSLGFQEQHPRRRRPETWE
                     DSAVTIMSHSRSEHPRELICPPGALGRLQPHQGHVVAADGNHTRTPVWFVPWFELAAE
                     EPSPGAHEGYASRYIESLMTRCGLNALLSSELVLATARLDEHLYEPALKQAEDFFRSK
                     ACLIPEGLESLPACWLAGLPGILWATRQPDWDRLPYMISRLECENGVLLLSFAQEQVS
                     EDERLTLWDELRVEVNRNLYPSRWRRLKRRHRMLLHFARPDLRTVLESSPGEMF"
     gene            complement(4477..>5809)
                     /locus_tag="VIL39_07110"
     CDS             complement(4477..>5809)
                     /locus_tag="VIL39_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481231.1"
                     /translation="QRVRQLKAEHLDWGASRIAQELGISVDLAKVHLARWTGAQIFEA
                     EQAKTAVAPDNVGAKTEPSPLPPVDAPHAGSTLQDTVGQSTRDLCYRGTQVNTLEDLL
                     VYAQVDTSVWEVERQVINKWEVGARNPATGEILTSPLFQIKIWLRRKVVETSLKDLTQ
                     SLLAEFKKEAPVRPARARSLGGGGLLEISIMDLHYGKLCWGEECGRDYNPEIAEKMFW
                     EALEDLLARSSGLKPEKILFPCGNDFYHTDVLGRTTTAGTPQDSAIVWKQAFVQGWTL
                     LAKAIERLSSVAPVQVVVVNGNHDVQSAFHLGEVLKAWFRTSGDVSVDNSPAQRKYVT
                     HHQCLLGLTHGSEEKHSNLGLLLATERPTDWAKSTPATREWHVGHFHAKKSLKLLPAI
                     DVGGVLVRVVPSLCPPDAWHASKGYGGRLAAEAYYWDPECGVTATLTHSPV"
BASE COUNT         1149 a         1833 c         1690 g         1137 t
ORIGIN      
        1 tgccaggtga tcaggtgcag cgcgggatcg gccagcactt tctcgaacac ttccgcgcca
       61 aatatcccgc gatccaccac cgtcgtgagc actcgctccg cgggccagtt cagcaccttg
      121 cggcaccgcg tcaggacggc aaagaagcgt tgccgcaaat cctcgaagtt gtcggtcgtc
      181 tcgaagtaaa tgggttcgcc tcggtgggtg tggataaagt cgctgtgcag cgctttatcg
      241 gccaagcgaa tgcccggaca ccaccctttg agcacatttt gctgcccggt gtagtgctcg
      301 gtatgaggat cgaaatataa atcgctgccc ggggacaagc cctgggcatt gaaccggaac
      361 aacgctgcga ccgtcgcctc ggtggacacc gacttgagca ggtcccgctg tggggccgga
      421 aaccgcacca ccgttcccaa aagcaactcc aggtcctgcc cgttgaggaa cttggtctgc
      481 tcgatgttca tcgcgcccaa aagcaaagtg gctgaccact ggcgcagcaa gggttgcggt
      541 ggctcgacca ccgccctgat ttgattgagc gcccgggcaa ataacaacac cccggcatgc
      601 tcacaccaga cactctggcc gggagtcgag ccgagcaaaa cgggagactc cttggggttg
      661 tgagtttgca gatcatcaag tgagtgggtg ttgataacca acgagttgga tgggcaatcc
      721 ccttccggtt cgcccccttc acaatcgcca ggggtctccc gtttttcggc cgccagcgcg
      781 gatgccctca acccgtccgc tacgggagac gctgacctgg ccgtgccgcc actcgcctcg
      841 ggatcatcct ccgattccac cgggccggtt ccagctttga gttcctgcac taacgggcgc
      901 aacgtctcgg gcgaaagatc cacgccgaat atctcgtgaa tctcctggcg cagacgctgg
      961 ctggatccgt aggtgccttg ggccgtgagc cgtggccaac gttggcgcac gaaggcttcg
     1021 atggtcggag ttagtttccg accggccgaa cgaccggccc atacccggac ccgttcctgc
     1081 gcgtcgccgg actgcaaagc ccggacccaa cgctgcaagg tcttgcgatc aacctcaaag
     1141 acctccgtca ggctctggac cttgagcccc gcatggaaca aacgaccgcc cagccgcttg
     1201 aacgccggag cttggggctg attgggtacg atctccaata gctcgaagcc ctagtaaaca
     1261 tgcaactgtt cctggtcgaa atcctcgtaa agcgcgaaga gcggattccg tttatcgatc
     1321 cccaaaatga gctggctgga agcaggagca tccgtaaaca tccagtcctt ctacaggacc
     1381 caaggacctt cggaaactcc gcatttcgcc gcccccacgc aagccagcta atccgggctc
     1441 ctttccggga attagacctg cgagttcttc tcggctgaaa aggcggtttt actgaggtct
     1501 gaaccaagtc cgttttcttc gctcggagaa attaccgtca aacaaatttg cgtgaataaa
     1561 ttaatcacgc ctaaaatcgg gccgcagatg atgggagacg catacacggt ctccgacctt
     1621 ctggcggcgt tatccactga acaattcaac aaactcattg gtttcgctcg gcttcggctc
     1681 cgcgccgtca ccagatccct tgagcttcga ggatgtctgg ccagcaccga tccagaagac
     1741 ttggtggccg aagcgttctt gaaactgaag ctcggcgagg acaacccctc gctggggcgg
     1801 cacctcaaag cgagaaaccg caccagtacg gaagccttcc tggaatccgt gcggggtatc
     1861 attaacagcg acctcgacca cctggttcag gaagccaaag cccgctatct gcacctgccg
     1921 gtgggcgatg tcgacaacga gccgggtgcg gtcgatcccg cagcgcccga ggatacttac
     1981 cagttgttat cgcggcggga tctgcagcag gtgctgtttg ccaaactcta tcagagcatc
     2041 cgcaaccagc ccgccttgct cggtattgtg cgggattggg aggagcgttt ccttgacgac
     2101 gaccacattg gtgtcgaggg ccaaaacgcc agcatggtgc atcgcgtccg ccagttcgcg
     2161 cgggagattc ttgccgagtt agcggctgaa ctctcgccga gtccgggcga cggaaaggag
     2221 ctgttcctat gattgccgac gatttctggg gcacgcccat cttccgctac acccgggccg
     2281 acgcattgcg tgacggcgtc ctgctggaca tctcggaaac cgccagcgtc tgcgcccggc
     2341 tccaggtgcc tgccgccatg acggttggac tgtgggatgt tctggctcct ggcgaaccgt
     2401 tcgacgtcca agacgagcgt ctaaaagtgg tgctctatgg cctcttactc gctgccatgg
     2461 gtataatgcc ccgtcgcatc ttcgacacgc cgagtgccca cattatggtc tacgtcctgg
     2521 aggtcgaatc caaggtggtc cgcgtgaagg cggcagcgca cttcgacgaa acggaccaac
     2581 ctgtctgcac cctcatgctt gagtcggagg actaaaacat ttcgcctggg ctggactcca
     2641 gtaccgtacg gagatccggc cgcgcgaaat gcaacagcat ccgatggcgc cgcttcaaac
     2701 ggcgccatcg gcttggatag agattacgat tcacctccac gcggagctca tcccaaaggg
     2761 tcaaccgctc gtcctccgac acctgttcct gggcgaagct cagcagcaaa actccgtttt
     2821 cacattccag ccggctgatc atgtagggga ggcggtccca atctggttgg cgggttgccc
     2881 acagaatgcc gggcaatccc gccagccagc acgccggcaa tgactccaaa ccttccggta
     2941 tcaggcacgc cttcgatcga aagaagtcct ccgcttgctt cagcgccggc tcatatagat
     3001 gttcatcgag tcgtgcggtt gccagcacga gttcagagga cagtagtgcg ttaagcccgc
     3061 accgggtcat gaggctttca atatagcgtg atgcgtagcc ttcgtgcgcg ccgggggatg
     3121 gttcctcggc agccagttca aaccacggca cgaaccaaac cggcgttcgg gtgtgattgc
     3181 catcagcggc aacgacatgc ccttggtgcg gttgcagtcg ccccaaggcg cccggtggac
     3241 aaatcaactc gcgcggatgt tcagaccgcg agtgcgacat gatcgtcacc gcgctgtcct
     3301 cccaggtttc gggacgcctc cgccgggggt gctgctcttg gaaaccgagg ctcatagctg
     3361 ccagctcccc cgcctcctcc tcgcgcgtcc gcatgaacac gagggtgttg taattcagca
     3421 acaccgcccg ccgcaggcga aatccgattt tgtcatctat cgccgccagt ccctgggttg
     3481 cggccagcac aaagcagcgc ttgctcctca gcgtggccag ttggtcggcg tcctccggcc
     3541 gcacgatgag cgggaactcg tcggcaatga gtccgcacag ggggtgctcg ccggtgccgc
     3601 gcgtctggac ggcatcgaag aactcgcgcc gtgccaggcg taggacgaac gcggccagtt
     3661 cggggtgaat caacgcattg atgctgacca cgcacagctt gccctccgcc accagggccg
     3721 gactgaacgc cgggcggtcg ttcgcatcga gatagcgcac ggccgccgcg ctcagcaacg
     3781 ggcgcagcac gttcaacagg cagctctgca ggttgcttcg cgtgcgcgga tcgagatgtt
     3841 tccaaacctc gacgtgatcc aacgccccga gcaactgagg tgccgagccg ggttttttag
     3901 cctcacgctt gccttgctcc acgacgtcgg ccaccggttt gggcaacgtt gcgaaggatt
     3961 cgaggccgac aaaccagctc cgcataaact caatcgcatc gccgaacgag atgggtcttt
     4021 tccgtaccaa caacagcgtg agggcagcag caatcattgc cgccgtggtg gtttgccagt
     4081 agggattgtc gcgcccgact ggttcggtcg ccagtaacaa ccgctgggtt accaaatcca
     4141 catcgccgag tgttttgagc gggccaaaga gatcaagtat gtgggtgcct tgaggaccaa
     4201 ggacaaccag gtcccgttca cgccccgccc gcccggcaac ctcaatgacg tgctccacag
     4261 tcccatcctg cttggcatca aggatgagca agccaatgcg tgactcatgg ctgatgagct
     4321 ggtccatcgc gccccgaagc aacgtggtct tgccgcagcc agtgctgcca acgaccagca
     4381 cgtgttggac aaggtcttcc tccgtgagcg cgatggtttg gccggccggc tcatagcagg
     4441 gccaaaccac caaaggtgtg ttggttggct tcatgttcac accgggctgt gggttagggt
     4501 ggcggtgaca ccgcattcgg gatcccagta atacgcctca gccgccagcc tgccgccata
     4561 gcctttggag gcgtgccacg catccggcgg acacaggctg ggcaccacgc gcaccagcac
     4621 gccgccgacg tcaatggccg gcaacagttt taagcttttc ttggcgtgga agtggcccac
     4681 gtgccactcg cgcgtggccg gggtgctctt ggcccagtcc gtcggacgtt cagttgccag
     4741 cagcaagccc agattcgaat gcttctcttc cgagccgtgg gtcaggccca gcagacattg
     4801 gtggtgggtt acgtatttgc gctgtgccgg tgagttgtcc accgacacat cacctgaagt
     4861 gcgaaaccac gctttaagca cctcgccgag atggaaggcg gactggacgt catggttgcc
     4921 attgaccacg accacttgaa ccggcgcgac cgagctcaaa cgctcgatgg ccttggccaa
     4981 cagggtccac ccttgcacga aggcctgctt ccagacgatg gccgagtcct gcggcgtgcc
     5041 ggccgtcgtg gtccggccca aaacgtcggt gtggtagaaa tcgttgccgc agggaaacag
     5101 aatcttttcg ggtttcaagc cggagctcct ggcgagcaga tcctccaatg cctcccaaaa
     5161 catcttctca gcaatctcgg ggttgtagtc gcgcccgcat tcctcacccc agcacagttt
     5221 tccatagtgc aggtccatga tgctgatttc cagcaggccg ccgccgccga gggaacgggc
     5281 gcgggccgga cgtaccggcg cctccttttt gaactccgcc agcaggctct gcgtcagatc
     5341 ctttaacgaa gtctccacca ccttgcggcg gagccagatt ttaatttgga aaagcggaga
     5401 ggttaagatt tcgccggttg ctggattgcg cgctcccacc tcccacttat tgatgacctg
     5461 ccgctccact tcccaaacgg atgtgtccac ctgcgcatac accagaagat cctccagggt
     5521 gttgacctgt gtgcctcgat agcacaggtc gcgcgtgctc tggcctacgg tatcctgcaa
     5581 cgtgctccca gcatggggag cgtccaccgg gggcaacggc gaaggttcgg tcttcgcgcc
     5641 aacattatcg ggggccaccg cagtcttggc ttgctcggcc tcaaagatct gcgcgcccgt
     5701 ccaccgcgcc aggtgcactt tggcaaggtc cacggaaatg ccgagttcct gcgcaatccg
     5761 gctggcaccc cagtccaggt gctcggcctt gagctggcgc acccgctga
//
LOCUS       DATCGS010000347         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_476389, whole genome shotgun sequence.
ACCESSION   DATCGS010000347 DATCGS010000000
VERSION     DATCGS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476389"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            721..1314
                     /locus_tag="VIL39_07115"
     CDS             721..1314
                     /locus_tag="VIL39_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481232.1"
                     /translation="MNNKKKAQGIDLIACSLARNLKLNSRAAKAEARRIRTLWHDALL
                     RGEPVDLPGLELQIVWGQQKQQLQPVPTGLLKSNRKTPIRLVKPGNSVKVSHRSTVYF
                     GFDVEGNVEQVAKPLDRSRRVKWMPGVSTPEQKPLQKKALAQKSVPAPTAQHGTAQAV
                     APRPIPQVVRAESLRPHQQQIIGSRTAMFRQNPGGRR"
     gene            1491..1799
                     /locus_tag="VIL39_07120"
     CDS             1491..1799
                     /locus_tag="VIL39_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481233.1"
                     /translation="MIQLRPSGTLVRAANRMPPRSKDTKASSLRIPKQPSARIPVLIA
                     QIFDKWPSRKFLQTVGRIAGLDSGRLLRILEEMVRCDMRYDPSFTVPFLDFLQQQFCL
                     "
     gene            1925..>2760
                     /locus_tag="VIL39_07125"
     CDS             1925..>2760
                     /locus_tag="VIL39_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481234.1"
                     /translation="MQKTGTKPTPTAAIEPHFTVPELSKLLNLDPRAVRRMFQDEPTA
                     VRIRTAAQPGKRAYTTYRIPESTALRVYRRHTGAGGDDAVPTQVDASPACGTVGSKPP
                     HEPYGEINMARRRSQQTGHVHRQGDGWYLAYREDAIGEDGKIVRVRMNRKIADAKEVS
                     KREAQRIAREILNTVDAQSQKPMSLLTVEEFVQRRFRPDVIRFKKPAGKSHYENMLKL
                     HVLPAIGGERLRDVTSDDVQDLVGAKLEAGFRDATEPLAAVALVFLLGLIAILIGFRL
                     KNG"
BASE COUNT          617 a          851 c          795 g          497 t
ORIGIN      
        1 taattacggc gctcgagctc ttcgagcacc cgttgacgaa gctgggtcat agggatctcc
       61 ttatgaccca gatttacctc tcccctccgt cggacgccag cccatacccg cacgcgtagc
      121 gtccgccgcg ccagcggctt acttgaaacg gcgttaccgg aaactggcga agcggctcag
      181 tctgacccag attagtaccg agaagtcggc ttgcgtccgt cgtactgtgc gaattcacgt
      241 ttttcgcagt ctctctgctg aatctaatgc cttttggggc tcgtggcgaa aaacctgcca
      301 gcgacgtgcg ggccgcacgc acgggccgta tcgcgttggc agcgagcgcg gatggaagcg
      361 gccgctatac aaaacctcca ttgggggttg gcacgcactt cttggcgagg cgcaactgct
      421 ggcaccgggc cctatgcagg cggacgggct attggctggg cgctttcact ttctgctaca
      481 catcgattcg acgcgacggt tacgactgaa tctgaacccg ctagccgaaa ccgatcgtcc
      541 actggacgat cttccccttc tggagaggcg catgcggaat cacgacgggt tgatgccggc
      601 atcacccact gattagccac cggccagcac cgcaaacggc gtgcgtgacc gccgccaaaa
      661 aggccgattc gtccacacgg cctacctata ctactgaatt ggtgaaggac acagaacgag
      721 atgaacaaca aaaagaaggc gcagggtatc gacttaatcg cctgctcact ggcgcgcaat
      781 ctcaagctga atagccgcgc ggccaaggcc gaagcccgcc gcatccgaac cctttggcac
      841 gatgcgctgc tgcggggcga acctgtcgat ctccccgggc ttgagcttca gattgtctgg
      901 ggacagcaga aacaacagct ccagccggtg ccgaccggcc tactcaagag taacagaaaa
      961 acgccgatcc ggctggtcaa gcctggaaac agcgtaaagg tgtctcaccg gtccacggtg
     1021 tacttcgggt tcgacgtgga ggggaacgtg gagcaggtgg ccaagcccct ggatcggagc
     1081 cgcagggtga aatggatgcc cggtgtttct acgcccgaac agaaaccctt gcagaagaaa
     1141 gcgctagccc agaaatcggt gccggcgccg acagcgcaac atggcactgc ccaggctgtc
     1201 gcgccacgcc cgatacccca ggtggtacgc gctgagtcct tacgtccgca ccagcagcag
     1261 atcatcggca gtcgcactgc gatgttccga cagaacccgg gaggcagacg atgaccagga
     1321 gtgatcttgt cagcgattgg agggagcgat ttatgatgac gccagcgcat gcgcagagct
     1381 gcgtcgacga attgtaccga gatttctgcg cggcgctcgc tacgggcggt gaagtacaac
     1441 tccggggtat cggcacatgg aagacagtgg tccgaaagaa cgggagaaga gtgattcagc
     1501 ttcgccccag cggcacgctg gtccgcgccg cgaacaggat gccgccgcga agcaaggaca
     1561 ccaaagcatc gtccttgcgg atccccaagc agccgtcggc acgcatcccg gtgctcatcg
     1621 ctcagatctt cgacaaatgg cccagccgca aattcctgca aacggttgga agaatcgctg
     1681 gcttggacag tggccgactc cttcgcatcc tggaggaaat ggttcgttgc gatatgcgct
     1741 acgaccccag cttcacggtc ccgttcctgg actttctcca acagcaattc tgcctatgaa
     1801 ctcttaccac attgcagcgg agagcacagt tccaggcgtc tggaagctag aaatctactt
     1861 aagtccaata ggttcaatgg tatagacgta atcaccacgg cgggtatact aatcagcttg
     1921 tgccatgcag aaaacgggca caaaacccac tccaacggcc gccatcgagc cgcatttcac
     1981 cgttcctgag ctgtccaaac tcctgaacct cgacccccgc gccgtccggc gcatgttcca
     2041 ggacgagccc accgccgtcc gcatccggac tgcggcgcaa ccgggcaaac gcgcctacac
     2101 cacctaccgc atccccgagt cgactgcgct ccgcgtctac cggcgccaca ccggcgctgg
     2161 tggcgatgac gcggtcccga cccaggtcga tgcctcgccc gcctgtggta ccgtaggcag
     2221 caagccaccc cacgagccat atggagaaat caatatggct cgcagaagaa gccagcagac
     2281 cggacacgtt caccgccagg gtgacggttg gtatctggcc tatcgtgaag acgccatcgg
     2341 cgaagatggc aagattgttc gcgtccggat gaatcgcaag atcgccgacg caaaggaggt
     2401 aagcaaacga gaagcccagc ggatcgcgcg agagattctc aacacggtcg acgcccagtc
     2461 gcaaaagccc atgtcattgt tgaccgtcga ggaattcgtc cagcgccgct tccggccgga
     2521 tgtgatccgg ttcaaaaagc ccgccgggaa atcccattac gaaaacatgc tgaagttgca
     2581 cgtgcttccc gcgatcggcg gggagcgtct cagggacgtc accagcgatg acgtccagga
     2641 tcttgtagga gcgaagttgg aggctggttt tcgggacgca accgaacccc ttgcagctgt
     2701 cgctctcgtc ttcctccttg ggctgatcgc catcttgata ggctttaggc taaaaaatgg
//
LOCUS       DATCGS010000348         3488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_375625, whole genome shotgun sequence.
ACCESSION   DATCGS010000348 DATCGS010000000
VERSION     DATCGS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375625"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..2150
                     /locus_tag="VIL39_07130"
     CDS             141..2150
                     /locus_tag="VIL39_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY5481235.1"
                     /translation="MKIIAPARERLARERLTRAARFALIALLATLVSLAAPSRAAVPA
                     SSPPAHSVADAMSLASPGTVQLQGWLGDKLGLCLNGRVWAQNPEALVAVIRSHHDKGD
                     WRGEYWGKWYSAAVLGYACQPTAERRAQLEQVALEVIKSQGPDGYLGSYDEKDRLTVW
                     DVWCRKYVLLGLLAAYDLTGDKTALEAARRDADNLIDDLDRRKIKLVEIGVQELKGVA
                     NSSIIEPMALLYQRTGDRKYLEFAQSIIAQWNAPYSTAPQGIHLLEKALAGSPPLQNH
                     AYAIMSCFEGICELYRATGDRQYLDGAVRFGQSVRRHERMIDGSASNHELFCEGARSQ
                     TEFLEKPQETCATVTWIKLCAQLLRLTGDPVWADEMELSLYNAMIGAMTPGGEWFSYF
                     TQLTGERVPSFIAHTDLNLSCCVANAPRGLLLTPRWAVMTAPDGPVVNLYAPGTASVK
                     LADGAEVRIVQETDYPVSERIMLTISPARKQRFTFRLRIPAWSQHTALAVNGQPVVCE
                     PGKYAKLDREWAPGDQVLLTLDLRGRTLPAPSGAPQLALMRGPILLALDNRLVPPTDT
                     AIWLSADAEGFVKLKPLAAKPAWAWMAFEVPFQVHPSHFYGHYTTNLALCDFSSAGND
                     WNEKNLYRTWLPQPLFLGNAFASHTWNLLNPHSATEPRPVIPTVR"
     gene            2147..2995
                     /locus_tag="VIL39_07135"
     CDS             2147..2995
                     /locus_tag="VIL39_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481236.1"
                     /translation="MRTFTQGRRPYTRQRVAPATGFTLIELLVVIAIIAVLAALLLPA
                     LSRAKAKGQGIHCLNNLKQLQLGWLMYAGDNQEAIPGDNWQDEAAHVKNAGNWLTGWM
                     APLDESATTDNTNTLFLLDVSYSQIGPYVKADGVYKCIADQSRARIGSLLLPRVRSVA
                     MNCWMGKNAPAWNSGYRTYAKTTDITAPGPSDAMVFLDERSDSLDDGYFAIDMDTAQL
                     VNFPAGYHNGASGITFADGHAEVHKWRDSRTLPPLQTTFHKFVDVSAPLSKDLIWLRQ
                     HATSKK"
BASE COUNT          710 a         1081 c         1049 g          648 t
ORIGIN      
        1 ggtcagcacc gacctgatgc gttggttcac cctgaccaat gctctgagct ggacgaacgg
       61 tttactgttg ctgcgcgacc cggatcgggc taaccatccc cgacggttct atcgtgtgct
      121 tgaacactga aggctgtgac atgaagataa ttgctcccgc aagagaaaga cttgcccgcg
      181 aacgtttaac ccgcgcggcc cggtttgcgc tgattgcatt gctggccact ttagtgtcgc
      241 tggctgcacc aagccgggca gccgttcccg ccagttcccc gccagcccat tctgtggccg
      301 acgccatgag tctggcctcc ccggggacgg tgcagttgca gggttggctt ggggacaagc
      361 tgggcttgtg cttgaatggc cgcgtttggg cccagaaccc cgaagcgctg gtagccgtta
      421 ttcgcagtca ccacgacaaa ggcgattggc gcggcgaata ttgggggaaa tggtatagcg
      481 cggcggtgct gggttacgcc tgccagccga cggcggagcg ccgcgcgcaa ctggagcaag
      541 tcgcgctgga ggtgatcaaa agccaggggc ccgacggtta tctcggctcc tacgacgaga
      601 aggaccgcct gacggtatgg gacgtctggt gccgcaagta cgtgttgctg ggattgctcg
      661 ccgcctatga cctgaccggg gacaagacag cactggaagc agcccgccgc gacgccgaca
      721 atctgattga cgacctcgac cgcagaaaga tcaagctggt ggagattggc gtgcaggagc
      781 tcaagggagt ggctaacagc tcgatcatcg agccgatggc cctgctctac cagcggaccg
      841 gcgaccggaa gtacctggag tttgcgcaat ccattatcgc ccagtggaat gcgccctaca
      901 gcacggcgcc gcaagggatt catctgctgg agaaggcgct ggcgggctcc ccgccgctgc
      961 agaatcacgc ctacgcgatc atgtcgtgct ttgaggggat ctgcgaacta taccgcgcca
     1021 ccggggaccg ccagtacctg gacggggcgg tgcgcttcgg ccagagcgtg cgccggcacg
     1081 aacggatgat cgacggttca gcctccaacc acgaactctt ttgcgaaggc gcgcgcagcc
     1141 agaccgaatt cctggagaag ccacaggaga cctgcgcgac ggtcacttgg attaagctct
     1201 gcgcccaact gctgcggctg accggggacc cggtttgggc cgacgagatg gagctctcgc
     1261 tctacaacgc catgattggc gcgatgaccc ccggcgggga atggttctcc tattttacac
     1321 agctcacggg cgagcgcgtg cccagcttca ttgcgcacac ggatcttaac ctgagctgct
     1381 gcgtggccaa cgcaccgcgg ggcctgctgc tgactccgcg ctgggcggtc atgacggccc
     1441 cggacggccc ggttgtcaac ctctatgcgc cgggaaccgc ctcggtcaag ttggccgatg
     1501 gcgcagaagt ccgaatcgtg caggagaccg attaccccgt gagcgagcgg atcatgctga
     1561 cgatatcgcc tgcccgcaag cagcgcttca ccttccgcct gcgtattccg gcatggagcc
     1621 agcacaccgc gctcgccgtc aacggtcaac cggtcgtgtg cgagccgggg aaatatgcga
     1681 agctggaccg ggaatgggct ccaggcgacc aggtcttgct cacactggac ttgcgcggac
     1741 gcaccttgcc agctcccagc ggcgcgccgc aattggcgct gatgcgcggg ccgattcttt
     1801 tggcgctgga caaccggctc gtgccgccaa ccgacacggc gatctggctc agcgcggacg
     1861 ccgagggctt cgtgaaactc aagccgctgg cggccaagcc cgcttgggcc tggatggcct
     1921 tcgaagtgcc cttccaggtt cacccctcgc atttctacgg ccattacacg acgaacctgg
     1981 cgctgtgcga tttcagctcc gctggtaatg attggaacga gaaaaacctc taccggacgt
     2041 ggctgcctca gccgctgttc ctggggaatg ccttcgcctc gcacacctgg aacctgctca
     2101 atccgcactc cgccacggaa ccgcgcccgg tgatcccaac agtaagatga gaacctttac
     2161 tcagggaaga agaccatata cccggcagcg tgttgcccct gcgaccgggt tcacactcat
     2221 tgaattgctg gtggtgatcg ccatcatcgc tgttttggcg gcgctgctgc tgcccgcttt
     2281 gtccagggcc aaggccaagg gccaaggcat ccattgcctt aacaatttga agcaattgca
     2341 actgggctgg ctgatgtatg ccggggacaa ccaggaagcc attcctgggg ataactggca
     2401 ggatgaggcg gcgcatgtca agaacgcggg caactggctg accggctgga tggctcctct
     2461 ggacgagtca gccaccaccg acaacaccaa cactctcttt ctcctggatg tcagctactc
     2521 ccagatcggg ccttacgtca aagcagacgg ggtctacaaa tgcatcgccg accaaagccg
     2581 tgctcgcatt ggcagcctgc tcctgccgcg agtccgcagc gtcgcaatga attgctggat
     2641 gggcaaaaac gccccggcat ggaatagcgg ttaccgcact tatgccaaga ccaccgatat
     2701 tactgcgcca gggccgagcg acgcgatggt gttcctggat gagcgctcgg atagcctcga
     2761 cgatggctac ttcgctatcg acatggacac ggcgcagctc gtcaatttcc ccgccggcta
     2821 tcacaacggc gcgagcggaa tcacttttgc cgatggccat gccgaggttc acaaatggcg
     2881 tgacagtcgc actttgcccc cgctccaaac caccttccat aaattcgtcg atgtatcggc
     2941 tcctctcagt aaggatctga tctggctccg gcaacacgcc accagcaaga agtgacccgc
     3001 cagcgaacct tgcccgcgga gcccaccgca ggccacggtg aaggctttcg agcgcgcatg
     3061 accagaactg gtcattgtgg cggagtcggc ttctgaggtt aaatccacca gtgaaagacg
     3121 cgtgctgcaa tgctaaatca ggccgctgtc aaccgtccac cggcgcggac ggcactcgtt
     3181 cacagttcgc caacagatca taaacacaaa ctcctgataa aaggaacgaa ccgaaagcat
     3241 tatgaaaaaa gccctaatcc ttgcagccgc ctgtctggtt gcttcgctag cgcaagccgc
     3301 gccggtcccg tcggggcaat ggcagcacag cctgaccgtc cggccctcta acactacagc
     3361 gtcatttagg acttgcgcct ccacggagat cggttaaagt gcatggagta attgaatggc
     3421 gctgggctgg agataatcga atcgcgagta attcgtggcc gaaccatcac ggctgcggat
     3481 gtagtggc
//
LOCUS       DATCGS010000349         8710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_225370, whole genome shotgun sequence.
ACCESSION   DATCGS010000349 DATCGS010000000
VERSION     DATCGS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225370"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..633)
                     /locus_tag="VIL39_07140"
     CDS             complement(220..633)
                     /locus_tag="VIL39_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEY5481237.1"
                     /translation="MRVVVDTNVVVSAILRDRLPERVILFIIARPDFEWVASREILTE
                     YREVLRRPKFGLPETVLSQWDDRFRAAVAEWPVEVPVSFPRDLKDAKFLACALAANAD
                     FFITGDQDFAEAGRLVRTKIVSVRQFDEMVLRPLQ"
     gene            complement(630..854)
                     /locus_tag="VIL39_07145"
     CDS             complement(630..854)
                     /locus_tag="VIL39_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481238.1"
                     /translation="MSTVDLICEKARALPDSLQSEALSFVEYLSRRGVAPSESADWQR
                     LARQTQALPAVERITDADITAEIAAYRAGK"
     gene            934..2616
                     /locus_tag="VIL39_07150"
     CDS             934..2616
                     /locus_tag="VIL39_07150"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY5481239.1"
                     /translation="MSANKYAIGLDYGTNSVRSLIVNVANGREVASAVWNYTHGTQGV
                     ILARDPNLARQHPADYITGAETTIKKALAVAKRTVRGFSPDQIIGIGVDTTGSTPLPV
                     DRHGHPLAFDRRFAKHPAAMAWLWKDHTGVAEAGEITALARKIRPQYLAKCGGTYSSE
                     WFFSKILHCLRTSPEVFEAAYLWVECADWVPAMLTGTEAPDQLTVGVCAAGHKAMFND
                     AWGGYPDAEFLSQLDPKLGELRSRLRPKAHTIDRAVGGLTLEWSKRTGLPEGLPVAVG
                     AFDAHLGGVGSGIAPGTLVKIIGTSTCDMMVVPAGDNKLADIPGLCGIVPGSILPGYY
                     GLEAGQSAVGDIFNWFVNYVQPLGKKAGSHESLSAAAAKLAPGESGLLALDWNNGNRT
                     VLVDQRLTGLLLGQTLYTTPAEIYRALIEATAFGALTIINRFEEYGVKVEQVVNCGGI
                     AEKNPLVMQIYADVTGRPIKISRSGQTCALGAAIAGAVVAGGYANYPDAQKAMTGLKS
                     RVFQPKPAAHAVYKELYSLYRKLHDAFGTQQQTGNLYDVMKQLIAIRNRARA"
     gene            2795..3949
                     /locus_tag="VIL39_07155"
     CDS             2795..3949
                     /locus_tag="VIL39_07155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007112824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY5481240.1"
                     /translation="MQINYQWLSILSTTLAAAALVGCTSTSQVPVQNGQISQRPFGQT
                     ADGIPVNLFTLRNSKGAEAAICNYGGLVVSLKVPDRSGHFGDVVLGYDNLAGYIKETP
                     YFGAMIGRYGNRIAKGKFTLDGKEYTLAVNNGPNALHGGLKGFDKVVWEPKIVASLEG
                     PSLELRYTSKDGEEGYPGTLSVKAVYTLTEDNALKLEYTATTDKDTVVNLTQHSYFNL
                     AGKGDILNHQVMLPADKFTPVDSTLIPTGELKPVSGTPFDFRTATAIGARIGQEDEQL
                     KFGNGYDHNWVINKPMGQLGLMGRVYEPTTGRVLEVLSTEPGLQFYTGNFLDGKLTGK
                     GGWAYQFRNGLCMEPQHFPDSPNQPNFPSVVLKPGQVYRHTLIFKFSVQK"
     gene            3982..5499
                     /gene="araA"
                     /locus_tag="VIL39_07160"
     CDS             3982..5499
                     /gene="araA"
                     /locus_tag="VIL39_07160"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY5481241.1"
                     /translation="MNTIDLSQFEVWLVTGSQNLYGAEVLRKVAANSAQVGRALDGAP
                     SIPVQVLVKPVVKTPAEASALCLAANESPHCIGLITWCHTFSPSKMWINGLKSLRKPL
                     LHLHTQHNRDIPWSSIDMDFMNLNQAAHGDREHGFIMSRLRLERKVVVGYWQDAAVQR
                     RIGAWCRAAAAWHDWQGAKFCRFGDNMREVAVTEGDKVAAQIQFGYSVNGYGVDDLVK
                     SVNAATDREIDRLVAEYESCYAVAPKLRKTGSKRQSLRDAARIELGLRAFLSAGDFKG
                     FADTFEDLPGLAQLPGLAAQRLMADGYGFGAEGDWKTCALVRAMKVMAAGLKGGTSFM
                     EDYTYHLDPKRSLVLGAHMLEICPSIAKGRPSLEVHPLSIGGKADPVRLVFDTLAGPG
                     LNASLIDLGNRFRLLANEVEVVPPDKPLPRLPVARAVWMPKPDLPTSAAAWIYAGGAH
                     HTGFSHAVTMEMIEDFAGMAGIELVVIDADTRLRQFKQELRWNEVYYALNRGFAG"
     gene            5503..6195
                     /locus_tag="VIL39_07165"
     CDS             5503..6195
                     /locus_tag="VIL39_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEY5481242.1"
                     /translation="MLEQLKRDVCQANLDLVKAGLVIQTWGNASGIDRRRGLVVIKPS
                     GVPYDGMMPKHMVVVSLQNGKVVEGRLKPSSDTATHLVLYRAFPSIGGIVHTHSLYAT
                     AWAQAKKGLPAYGTTQADYWHGEVPCTRLLTTAELQSDYEANTGHIIVERFKQLDPSE
                     HPAVLVASHGPFAWGKTVADAVHNAEVLEFIARLASETLRLNPKIKPMPPALLDKHFL
                     RKHGAHATYGQS"
     gene            6238..8037
                     /locus_tag="VIL39_07170"
     CDS             6238..8037
                     /locus_tag="VIL39_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY5481243.1"
                     /translation="MSPHTALSVNASGPTLTGLDWLAIVFYFSILFCVAWWVIHRAKD
                     TAADYFLAGRNLGWWVIGASIFASNIGSEHIVGLAGSGAKDGVAMAHYELHAWCLLVL
                     AWVFVPFYARSMVFTMPEFLERRFSTSSRYVLSIVSLVTFIVSKIAVGIFAGGVVFST
                     LLPEIHLSLGAVEINSFWIGSVAVIVLTGLYTALGGMRAVAYNDAVQVVVLICGSATL
                     TFYGLHKLGGWTELRHLCGSDMFNLWKPLTPPGVEATWAPVMVRNDSGQLVKQAWYFN
                     QHYPWLGMAFCAPVIGLWYWCTDQYIVQRALGAPNQTIARQGSIFAAFLKLFPVYLFI
                     IPGLICFALVKSGKVPELAANFDAQNAFPLMVKHLLPPGLRGIVVAGLLSALMGSLAG
                     IFNACSTLFTVDLYEKWKPKATQHELVRTGRIATTVMVIIAMLWIPVVQGANSLYDYL
                     QSVQGYLAPPIFVVFFFGVFWKRLNAQGCLYTMVVGFALGLFRMLVDTPVTMKLKGFE
                     HGYPQGSFLWIINNINFQYFSIIITLVSALVMVGVSYATPAPQYERIKSLTFGTTTDE
                     DRAKTSASWGWQEVAASVFVLLCIIGAYIYFRG"
     gene            complement(8103..>8710)
                     /locus_tag="VIL39_07175"
     CDS             complement(8103..>8710)
                     /locus_tag="VIL39_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5481244.1"
                     /translation="AAGPSRWVIASIGPTGKMLVTGEVTEEDLYAAFKEQAMALAKGG
                     APALCIETMSAADEAALAIRAARENTDCEVICTFTFQRGAKGQFRTMMGLSPTKAAEE
                     AIKAGAHIVGPNCGNGIEYMIEIVKEMRAAAGATPILVHANAGLPKTVNGVDVFPESP
                     EEMAARVEAIVEAGANIVGGCCGTTPAHIKAIRKAVDGLRR"
BASE COUNT         1670 a         2827 c         2590 g         1623 t
ORIGIN      
        1 cgacgaagtt cggcaccgcg agagaggcca ggaatgccac gaacagccct agcgccaaag
       61 ggcctcccca tcttaaggtg aaaatgcaat atctttccgg gagcttactc ataatgcact
      121 ccgcatcgtc gcccatgaaa gcgcggcggc agctttttgc aaggagaatt gcccgatgcc
      181 tccggacggc cttctgctat gatcgatccg ccttcaacgt cattgcaacg gccggaggac
      241 catttcatcg aattgcctga cggacacgat cttcgtgcgg acgagccttc cggcctcggc
      301 aaaatcctgg tcgccggtga tgaagaagtc cgcgttggca gccaaggcgc aggcgaggaa
      361 cttggcatcc ttaaggtctc gggggaaaga gaccggaacc tccaccggcc attcggcgac
      421 ggcagcgcga aagcggtcat cccattgaga cagcacggtc tcgggcaatc caaactttgg
      481 ccggcgcagg acttcgcggt attcggtcag aatttcccgt gaagccaccc attcaaaatc
      541 ggggcgggcg atgatgaaga ggatcacccg ttcgggcagt cggtcgcgga ggatggcgga
      601 gacgacgacg ttggtgtcaa cgacgaccct catttgccgg cgcggtaggc ggcgatctca
      661 gcggtgatgt cggcgtcggt gatcctctct acggcaggca aggcttgggt ttggcgtgcg
      721 aggcgttgcc aatcggcgga ctcgcttgga gcaaccccgc gccggctcag gtattccaca
      781 aaactcaacg cctccgactg caggctgtca ggaagtgctc ttgccttttc gcagatcaaa
      841 tcaaccgtgc tcatgggcca aaggtagcgt gagcacggcg cggtatcaag ctttgctgga
      901 cacgcgccgt cgtgctgatt atgtaaaggg gccatgagcg caaacaaata cgctatcggc
      961 ctcgactacg gcaccaactc cgtccgcagt ctcatcgtta acgtcgccaa cgggcgcgag
     1021 gtggcgtccg cggtttggaa ttatacgcac ggcacgcagg gggtcatcct cgcgcgcgac
     1081 ccaaacctcg cccggcagca tccggccgac tacatcaccg gcgcggagac cacgatcaag
     1141 aaggccctcg cggtggccaa acggacggtg cgcggcttct cgccggatca gatcatcggc
     1201 atcggggtgg ataccacggg cagcacgccg ttgccggtgg atcgccacgg ccacccgctc
     1261 gccttcgacc gccggttcgc caagcacccc gcggcgatgg cctggctgtg gaaggaccac
     1321 acgggcgtgg ccgaggcggg ggagatcacg gcgttggcgc gcaagattcg cccgcaatac
     1381 ctcgccaagt gcggcggcac gtattccagc gaatggttct tcagcaaaat cctccattgc
     1441 ctgcggacca gccccgaggt gttcgaggca gcttacctgt gggtggaatg cgccgattgg
     1501 gtccccgcga tgctcaccgg caccgaggcg ccggaccaac tgaccgtcgg ggtctgcgcc
     1561 gccggccaca aggcgatgtt caacgacgcc tggggcggtt acccggacgc cgagtttctc
     1621 tcgcagcttg atccgaagct gggcgagctg cggtcacggc tgcggccgaa ggcgcacacg
     1681 attgaccggg cggtgggcgg gttgacgctg gaatggtcca aacgcaccgg cctgccggag
     1741 ggtctgccgg tggcggtggg agcgttcgac gcgcacctcg gcggcgtcgg ctccggcatc
     1801 gcaccgggca cactcgtgaa gatcatcggc acgagcactt gcgacatgat ggtcgtgccg
     1861 gcaggcgaca acaagctggc ggacatcccc ggcctgtgcg gcatcgtccc cggcagcatc
     1921 ctgcccggct attacggctt ggaagcgggc cagtcggcgg tcggcgacat cttcaactgg
     1981 ttcgtgaact acgttcagcc gctcgggaag aaggccggtt cgcatgagtc gctcagcgct
     2041 gcggcggcca agttggcccc aggtgaatcc ggcctgctgg cattggattg gaacaacggc
     2101 aaccgcacgg tgcttgtgga ccaacgcctc actggcttgc tgctcggtca aacgctctac
     2161 accacgccgg ccgagatcta ccgcgcgcta atcgaggcca cggcgttcgg ggcgctgacc
     2221 atcatcaacc gcttcgagga atacggcgtc aaggtggagc aagtcgtcaa ctgcggcggc
     2281 atcgcggaga agaacccgct ggtcatgcaa atctatgccg acgtgactgg ccggcccatc
     2341 aagatctcgc gctcggggca gacctgcgct ttgggcgcgg ccattgccgg ggcggtagtc
     2401 gcaggtggct atgccaatta ccccgatgcc cagaaggcga tgaccgggct gaagtcccgc
     2461 gtcttccagc ccaagccggc agcccatgcc gtgtataaag agctttattc cctttaccga
     2521 aagctgcacg atgcctttgg aacacaacag cagaccggaa acctgtatga cgtgatgaag
     2581 cagctaatcg ctatccgcaa ccgagcgcgg gcctgaggcc ggcacggtgt taaccacgaa
     2641 atacacgaaa cacactaaaa gaaggaccga agagaatgga taccaatatc atctacaagg
     2701 aggaaagcta ccggatcatc ggggcttgct ttgaggttta tttagtgtat ttcgtggttt
     2761 agaactcaac aaccaactgc aaagaagaat caatatgcaa atcaattatc aatggctaag
     2821 tatcctctcc acaactttgg cggcggctgc gcttgtcggc tgcaccagca catcacaggt
     2881 cccggttcag aacgggcaga tcagccagcg cccctttggc cagacggcgg acggcatccc
     2941 cgtgaacctc ttcaccctgc ggaatagcaa gggggccgag gccgccatct gcaactacgg
     3001 cggcctggtc gtctccctca aggtgcccga ccgcagcggt cacttcggtg atgtggtgct
     3061 cggctacgac aacctggccg gttacatcaa ggagacccct tacttcgggg ccatgatcgg
     3121 gcgttacgga aaccggattg ccaaaggcaa gtttacgctc gacggcaagg aatacaccct
     3181 ggcagtcaat aatggtccca acgccttgca cggcggcctt aaaggcttcg acaaggtcgt
     3241 ctgggaaccc aaaatcgtgg cgagcctgga aggcccctcg ctcgagcttc gctatacgag
     3301 caaggacggc gaggaaggct acccgggcac cctgtcggtc aaagcggttt atactctcac
     3361 cgaagacaac gccttgaagc tggaatacac cgcgaccacg gacaaggaca cggtcgtgaa
     3421 ccttacgcag cattcctatt tcaacctggc cggcaaaggc gacatcctga accaccaggt
     3481 catgctgccg gcggacaagt tcacgccggt ggacagcacc ctgatcccga cgggcgagtt
     3541 gaagccggtg agcggcacgc cctttgattt ccggaccgca acagccatcg gagcacgcat
     3601 cggccaggag gacgaacagc ttaagttcgg caacggttac gaccacaact gggtcataaa
     3661 caaaccgatg gggcagctcg gcctcatggg gcgcgtgtat gagccgacca ccggccgggt
     3721 gctggaggtt ctatccaccg agccgggcct gcagttctac accggcaact tcctcgacgg
     3781 caagctcacg ggcaaaggcg gctgggccta tcagttccgc aatggcctct gcatggaacc
     3841 gcagcatttc ccggattcgc ccaaccagcc gaacttcccc tctgtggtgc tgaagcccgg
     3901 ccaggtgtac aggcacaccc tcatcttcaa gttctcggtg cagaagtaga tcccgggatc
     3961 caaccaccac cctggtgaat catgaacaca attgacctga gccaattcga ggtctggctg
     4021 gtcacaggca gccagaatct atacggggcc gaagtgctga gaaaagtcgc ggcgaactcc
     4081 gcgcaagtcg ggcgggcgct cgatggggcg ccatccatcc ccgtccaggt gctggtcaaa
     4141 ccggtggtca agacaccggc ggaagcgagc gcgctgtgcc tggccgccaa cgagagtccc
     4201 cactgcattg gcctgattac gtggtgccat actttctccc cgtcgaagat gtggatcaat
     4261 gggctcaagt cgctgcgcaa gcccctgctg cacctgcata cccagcataa ccgggacatc
     4321 ccctggtcgt ccattgacat ggacttcatg aacctcaacc aggccgccca cggcgaccgc
     4381 gagcacggct tcatcatgag ccgcctgcgc ctggagcgga aggtggtggt cggctactgg
     4441 caggacgcgg cggtgcagcg gcgcattggc gcctggtgcc gcgcggcggc ggcctggcat
     4501 gactggcagg gcgcgaagtt ctgccggttc ggcgacaaca tgcgcgaggt cgccgtaacc
     4561 gagggcgaca aagtcgccgc ccagatacaa ttcggctact cggtcaatgg ctacggcgtg
     4621 gacgacctgg tcaaatctgt caacgcggca acggaccggg agattgaccg tcttgtcgcc
     4681 gaatacgaga gctgctacgc ggtggcgcct aaactccgca agaccggctc caaacggcaa
     4741 tccctgcgcg acgcggcgcg catcgagtta ggcttgcgcg ccttcctaag cgccggcgac
     4801 ttcaaggggt tcgcggatac ctttgaagat ttgcctgggc tggcgcaact gcccggcttg
     4861 gccgcgcagc gtcttatggc cgacggctat ggctttggcg cggaaggcga ctggaagacc
     4921 tgcgcgctcg tgcgcgccat gaaggtcatg gccgcaggac tcaagggcgg cacgtccttt
     4981 atggaggatt acacctacca cttggaccct aagcgcagcc tggtgctggg cgcgcacatg
     5041 ctggaaatct gcccgagcat cgccaaaggc cgcccctcgc ttgaagtgca tccgctcagc
     5101 atcggcggca aggccgaccc cgtgcggctg gtcttcgaca cgctcgccgg cccggggctc
     5161 aacgcttcgc tcattgactt gggcaaccgc ttccgcttgc tggcgaacga agtggaggtt
     5221 gttcccccgg acaagccgct gccccggctt ccagtcgccc gcgcggtctg gatgccgaaa
     5281 cccgaccttc cgacttctgc cgcggcctgg atttacgcgg gcggcgcgca ccacaccggc
     5341 ttcagccacg cggtcaccat ggagatgatc gaggactttg ccggcatggc gggcatcgag
     5401 ctggtggtca ttgacgccga cacgcggctc cgccaattca agcaggagct gaggtggaac
     5461 gaagtctatt atgccttgaa ccgcggtttt gccggttagc gcatgctgga gcaactgaag
     5521 agagacgtct gccaggcaaa cctggacctg gtcaaagcgg gtctggtcat ccagacctgg
     5581 ggcaatgcca gtgggataga ccgccggcgc gggctggtcg tcatcaagcc ctcgggtgtg
     5641 ccctatgacg gcatgatgcc caagcacatg gtcgtcgtct cgctccagaa cggcaaagtc
     5701 gtcgagggca ggctcaagcc gagttcggac accgccaccc acctcgtcct ctaccgcgcg
     5761 ttcccgagca tcggtggcat cgtccacacc cacagtctct acgccaccgc ctgggcccag
     5821 gcgaagaaag gcctgcccgc ctacggcacc acgcaagccg actactggca cggcgaggtg
     5881 ccctgcacac gcctgctcac gacggccgaa ctccaatccg attacgaagc caacacgggc
     5941 catatcatcg tcgagcggtt taagcagctt gaccccagcg agcatcccgc cgtgctggtg
     6001 gccagtcacg gcccgtttgc ctggggcaag accgtggccg atgcggtcca caacgctgag
     6061 gtgctggagt tcatcgcccg ccttgcgagt gaaaccctgc gcctcaaccc gaagatcaaa
     6121 cccatgccac ccgctttgct cgacaaacac ttcctgcgca aacacggagc ccatgccacc
     6181 tatggccagt cctgatgtaa ccatgtaacc cttcaaccct gtaacccctt acccaccatg
     6241 tctccccata ctgccctatc cgtcaacgcc agcggcccca ctctcaccgg cctcgactgg
     6301 ctggcaattg tcttctactt cagcatcctg ttctgcgttg cctggtgggt catccaccga
     6361 gccaaggaca ccgccgccga ctacttcctc gccgggcgca acctcggctg gtgggtcatc
     6421 ggcgcgtcca tcttcgcctc caacatcggc tccgagcaca tcgtcggcct ggccgggtcg
     6481 ggggcgaaag acggggtggc gatggcccat tacgaactgc atgcctggtg cctgctcgtg
     6541 ctggcgtggg tgttcgtccc gttctacgcc cgctcgatgg tcttcaccat gccggagttt
     6601 ctcgaacggc gcttctccac cagctcgcgc tacgtgctgt ccatcgtttc gctcgtcacg
     6661 ttcatcgtgt cgaagatcgc ggtgggtatt tttgcgggcg gagtcgtgtt ctcgacgctc
     6721 ctgccggaaa tccacctgag ccttggcgcg gtcgagatca atagcttctg gatcggctcg
     6781 gtggccgtga ttgtgcttac cggcctttac accgccctgg gcggcatgcg cgccgtcgcc
     6841 tacaacgacg ccgtccaggt cgtcgtgctc atctgcggtt cggccacgct gaccttctac
     6901 ggcttgcaca agttgggtgg gtggactgaa ctgcgtcacc tctgcggctc ggacatgttc
     6961 aacctctgga aaccgctgac gccccccggc gtcgaggcca cctgggcgcc cgtgatggtg
     7021 aggaacgact ctggacaact cgtcaaacag gcctggtatt tcaaccagca ttacccctgg
     7081 ctcggcatgg ccttctgcgc cccggtcatc ggtctctggt attggtgcac tgaccaatac
     7141 atcgtccagc gcgccctcgg cgctcccaac cagaccatcg cgcgccaggg cagcatcttc
     7201 gccgcgttcc tcaagctctt ccccgtctat ttattcatca tccccggcct gatctgcttc
     7261 gcgctggtca agagcggcaa ggtgccggaa ctggcggcga attttgacgc ccagaacgcc
     7321 ttcccgctca tggtcaaaca cctcttgccc ccaggcctgc gcggcatcgt cgtggccggg
     7381 ctgctctccg ccctgatggg ttcgctcgcc ggcatcttca atgcctgctc caccctcttc
     7441 accgtggacc tctacgagaa atggaagccc aaggccaccc aacacgaact cgtccgcacc
     7501 ggccgcatcg ccaccacggt catggtgatc atcgccatgc tgtggattcc ggtcgtgcag
     7561 ggcgcgaaca gcctgtatga ctacctgcag tcagtgcaag gctacttggc gccgcccatt
     7621 ttcgtcgtct tcttcttcgg cgtcttctgg aagcgcctca acgcccaagg ctgcctttac
     7681 accatggtgg tgggcttcgc cctcggcctg ttccgcatgt tggtggacac accggtaacg
     7741 atgaagctca aaggctttga gcacggctat ccccaaggca gtttcctgtg gatcatcaac
     7801 aacatcaact tccaatactt cagcatcatt atcacgctcg tctcggccct ggtgatggtc
     7861 ggcgtgagct acgcgacccc ggcgccccaa tatgagcgca tcaagagcct cactttcggc
     7921 accaccaccg acgaagaccg agccaagacg agcgccagtt ggggctggca ggaagtggcg
     7981 gcgtccgtat tcgtcctcct ctgtattatc ggcgcttaca tttacttccg gggatagcaa
     8041 cataacgtga ccaagacgcg gaagacgggt tttggcccgc gctacccggt gtaaggcgca
     8101 tttcaccttc gcagtccgtc aacggccttt cgtatggctt tgatatgggc cggggtggtc
     8161 ccacagcagc cgcccacgat gttcgctccc gcttcgacga tcgcttcgac tctcgccgcc
     8221 atttcttcag ggctctcggg gaaaacgtcc acgccgttaa ccgtcttcgg cagccccgca
     8281 ttggcatgga ccaggatggg cgtcgcgccc gcggcggccc gcatctcctt cacaatctcg
     8341 atcatgtatt cgatgccgtt gccgcagttc gggccgacga tatgcgcgcc cgccttgatg
     8401 gcttcctcgg ccgccttggt cggcgaaagc cccatcatgg tgcggaactg gcctttggcg
     8461 ccccgttgga aggtgaaggt gcagatgact tcgcaatccg tattctcgcg ggccgcgcgt
     8521 atcgccaggg cggcttcatc cgccgcgctc atggtctcga tgcacaaggc gggagcgccg
     8581 cctttggcca gcgccatcgc ctgttccttg aatgccgcgt aaaggtcttc ctccgtcacc
     8641 tcacccgtca ccagcatttt ccccgtcggc ccgatcgagg ctatcaccca ccgggacggt
     8701 cccgccgcct
//
LOCUS       DATCGS010000350         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_624443, whole genome shotgun sequence.
ACCESSION   DATCGS010000350 DATCGS010000000
VERSION     DATCGS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624443"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..>3064
                     /gene="xdhA"
                     /locus_tag="VIL39_07180"
     CDS             129..>3064
                     /gene="xdhA"
                     /locus_tag="VIL39_07180"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY5481245.1"
                     /translation="MSLPVMLSAIEFRLNGHTVRVKDVSPNTTLLEYLRGAGLTGAKE
                     GCAEGDCGACTVAIMDRDSQGRPCYRAINSCLVPVCLMAGCEIVSVEGVASPAKLHPV
                     QERMVEGHGSQCGYCTPGFVMSLFEGYYRGDIRKTDQLDGQLCGNLCRCTGYRPIREA
                     AMEAFPERQRKHGQDLFAERLKTSKTGLDGVEYEFAGERFFRPASLGGLLQLLAQFPE
                     GRLIAGATELGLDITKRFRKFPTLVSVEAVPELKEIQRTDAEWRIGAAVTLTQIEEQM
                     ADEFPALGDMLRVFGSRQVRNRATMGGNLVTASPIGDSAPLLLALDARVVLASLNSGA
                     GVSPALLSERTLPIHEFFVAYRKTALRPGEILKTIIVPRFTQTGVTRKCAWYKVSKRR
                     EMDISTVAACFTVDLDSDGVVRHARLAYGGVAAMPTRARQAEQALLGQRWCEKMVKDV
                     LPTLQAEFTPISDVRGSAEYRRALITGLFEKFFSSGGDEAAGLAVYPKHQSLLRSAPA
                     RKELRPPPHESAHKHVTGEAVYTDDQPPGALVVWPVCSPHAHARILSRDATAARSMPG
                     IRAVLLAEDVPGENNVGGVKHDEILLPDKAALFHGQIVALVVGQSQEACRAAAEKVVV
                     EYEPLPPILTLEQALAARSFHNAPNYIRRGKVGEALADSPMTLTGVFELQGQEHFYLE
                     TQAAWAKPGEDGAMFVSSSTQHPSEVQAVVSHLLGVAANKVVVQSLRMGGGFGGKETQ
                     GATPAALAALAAYCTGQPVCVRWNRDQDMMLTGHRHPFLARFKVGFDAEGRLLAARVH
                     LYSNGGWAMDLSQAITDRALFHLDNAYYIPAVEFRGQVAKTNLSSNTAFRGFGGPQGM
                     VVMEEILDRIARRLGLPPEAVRERNLYHGKGQTNTTPYGQEIEDNRIQTIWRQLKLSS
                     DFARRREAVARWNAAHPHRKRGLAITPVKFGISFTVTFLNQAGALVLVYQDGTVQVNH
                     AGTEMG"
BASE COUNT          545 a          939 c         1021 g          559 t
ORIGIN      
        1 tgaacggagc cgcgtcacga cctcgtggct gcggacgcct ggtccttatc tcagacttca
       61 gcctttagcc tctggtgctg cgcaaaagaa ccggtatttc gtttacaggc ccccgttgcg
      121 ggagtattgt gagcttacca gttatgttga gcgccatcga attccggctc aatggccaca
      181 ctgtccgcgt caaggacgtc tccccaaaca ccaccctgct cgaatacctg cgcggggcgg
      241 gtttgaccgg cgccaaagag ggctgtgcgg agggcgattg cggcgcgtgc acggtggcaa
      301 tcatggaccg tgattcgcag ggccggcctt gctaccgggc catcaatagt tgcctggttc
      361 ctgtgtgcct gatggccgga tgcgagatcg tcagcgtgga aggcgtcgcc agccccgcca
      421 agttgcatcc cgtccaggag agaatggttg agggccacgg ctcccaatgc ggctactgca
      481 cgccgggctt tgtcatgtcg ctcttcgagg gctattatcg gggggacatt cggaaaaccg
      541 accagcttga cggtcagtta tgcggcaatc tctgccggtg cacgggctat aggccgattc
      601 gcgaggcggc tatggaggcc ttcccggagc ggcaaaggaa gcatgggcag gatttattcg
      661 ccgagcggct gaagacgtca aagacgggct tggacggggt cgagtatgag ttcgccgggg
      721 agcgattctt ccggccagct tcgctcggcg gcttgctgca gttgcttgca caattccccg
      781 agggccggct catcgccggc gccactgagc tggggctcga tatcacaaag cgcttcagga
      841 agttccctac gctggtctcc gtggaagcgg tgccggagtt gaaggagata cagcgcaccg
      901 atgccgaatg gcgcatcggc gctgccgtca cactcacgca aatcgaggag cagatggcag
      961 acgagttccc ggcgctgggc gacatgcttc gcgtcttcgg ctcgcggcag gttcgcaacc
     1021 gcgcgaccat gggcggcaac ctcgtcaccg cctcgcccat cggcgatagc gccccgctgc
     1081 tcctcgcact cgatgccagg gtagtgctcg cctcgctaaa tagtggggca ggcgtctcgc
     1141 ctgccctgct ttcagagcgc accctcccga tccacgagtt cttcgtcgcc taccgcaaga
     1201 cggctctgcg gccaggggag attctcaaga ccatcattgt gccgcggttt acgcaaacag
     1261 gcgtcacgcg caagtgcgcc tggtacaagg tctccaagcg gcgcgagatg gacatcagca
     1321 ccgtggcggc gtgcttcacg gtggatttgg actcggacgg tgtcgttcgt cacgcgcggc
     1381 tcgcttatgg cggagtggcc gcgatgccca cgcgggcgcg ccaggcggag caggcgctgc
     1441 tcggccagcg gtggtgcgag aagatggtca aggatgtgct gccgacgctt caggctgagt
     1501 tcacgccgat ttccgatgtg cgcggcagcg cggagtatcg gcgcgcgctg atcacagggt
     1561 tattcgagaa gttcttttcc tccggaggcg acgaagcggc agggcttgcc gtttacccaa
     1621 agcatcagag cctccttagg tcggctcctg cacggaagga gttgcggccg ccaccgcacg
     1681 agagcgcgca caagcatgtg acgggcgagg cggtttacac cgatgaccag cctccggggg
     1741 cgctcgtggt ttggccggtc tgttccccgc acgcccatgc cagaattttg agccgcgacg
     1801 cgacggccgc gcgctccatg cccggcatcc gcgcggtgct cctggcggaa gatgtgcccg
     1861 gcgagaacaa tgtcggcggg gtcaagcacg acgaaatcct gctgcccgat aaggcggctt
     1921 tgttccacgg ccagatcgtg gcgctggtgg ttggccaatc gcaggaagcc tgtcgagcgg
     1981 cggcggagaa ggtggtggtc gaatacgagc cgctaccgcc catcctgacg ctggagcagg
     2041 cgctggcggc gcgcagtttc cacaacgcgc ccaactacat ccggcgcggc aaagtcggcg
     2101 aggcattggc cgattcgccc atgacgttga cgggagtgtt tgagctgcaa gggcaggagc
     2161 atttctacct ggaaacgcag gcggcgtggg ccaagcctgg cgaggacggg gccatgttcg
     2221 tttcgtcctc cacccagcat ccttccgagg tgcaggccgt cgtctcccac ttgttgggcg
     2281 tcgccgccaa taaggttgtg gtgcaaagcc tgcgcatggg cggcgggttc ggcggcaagg
     2341 aaacgcaagg ggccactccg gccgcgctcg cggcgctggc ggcgtattgc actggccagc
     2401 ccgtgtgcgt gcgctggaat cgcgaccagg acatgatgct gaccgggcat cggcatccgt
     2461 tcctcgcacg gttcaaggtg ggctttgacg ccgaaggccg gttgctggcc gcgcgggtgc
     2521 atctctattc caatggcggc tgggcgatgg atctgtcgca ggccatcaca gaccgcgcgt
     2581 tgttccatct cgataacgcc tactacatcc cggccgtcga gtttcgcggg caagtggcca
     2641 agacgaacct gtcctcgaac accgcgttcc gcgggttcgg cggcccgcaa ggaatggtgg
     2701 tcatggaaga aatcctcgac cggattgccc ggcggctggg cttgcccccg gaggctgtgc
     2761 gcgaacgaaa cctttatcac ggcaaaggcc agacgaatac gacgccctac gggcaggaga
     2821 tcgaggacaa ccgcattcaa accatctggc gccaactgaa gctgtcgagc gacttcgcgc
     2881 gccgccgtga agctgtcgcc aggtggaatg cggcgcaccc gcaccgcaaa cgcggcctgg
     2941 ccatcacacc ggtcaaattc gggattagct tcacggtgac gttcctcaac caggccggcg
     3001 cgctggtgtt ggtctatcag gacggcacgg tacaggtgaa ccatgccggc accgaaatgg
     3061 gcca
//
LOCUS       DATCGS010000351         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_574856, whole genome shotgun sequence.
ACCESSION   DATCGS010000351 DATCGS010000000
VERSION     DATCGS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574856"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VIL39_07185"
     CDS             complement(<1..95)
                     /locus_tag="VIL39_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY5481246.1"
                     /translation="MSIKPSGKPVVGIIMGSDSDWPTMKAAAGVCA"
     gene            187..1458
                     /locus_tag="VIL39_07190"
     CDS             187..1458
                     /locus_tag="VIL39_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481247.1"
                     /translation="MKWIASESRTTVGRWRSIAEFLALRRNTSLLLVALVLAGTGEKL
                     WLGFAPKYLQTLGASIFVIGLFDALQTLLGALYAYPGGWLTDRWGQRRSLMLFSVLSL
                     AGYTLVLVWRHWLALLLGTFLFLAWSALSLPTTFTVVATSLKARQHTMGIGIQSMVRR
                     VPMMLGPLGGGWLITRFGWTRGIQCALLLCLLMSLLTMVFQWFMLEPGQGESPPTTES
                     GPTNFFAVTRSFTPALRELLVSDILIRFCERIPYAFVILWAMNHAGVDAQQFGVLVAC
                     EMVTAMVCYIPVAHLADKYGRRPFVLVTFVFFTLFPLTLLWGDSFGWLVLAFVVRGLK
                     EFGEPSRKALIIGEAVPALRARTYGAYYLIRDCVVTSGSFLGAWLWSIGPRANFIGAA
                     VCGALGTAWFWWFIYRRKPAAGLTGGQKGAN"
     gene            1473..2075
                     /locus_tag="VIL39_07195"
     CDS             1473..2075
                     /locus_tag="VIL39_07195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5481248.1"
                     /translation="MTTANKITILRILLVPFFVVEVLYYTKQGHEVHRLLAIVSFALA
                     SICDGVDGYVARRFNQRSELGAILDPLADKLLLVSGIVVLSFDHSPYLESIPLWLTGT
                     IIGRDVLILIGMVVIHLMVGKVKVRPRIIGKIATVLQMVVVLWIMLKWGESWLRALCL
                     GTAICTGISGLLYVWDGSQQLSAHPSSSPASNVESPKPKV"
     gene            2935..3639
                     /locus_tag="VIL39_07200"
     CDS             2935..3639
                     /locus_tag="VIL39_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481249.1"
                     /translation="MKESPDSCEYMPVRNDDVLAFGAEDPAATRSSLVKRLRNWEDHG
                     SWQDFFDTYWKLIYAVAIKAGLSEAEARDVVQETVLAVAKQMRENGYDRAKSSFKNWL
                     CLITRRRIIDHFRKRTDPKVRPSPRARDGTSRTDTINRVPDPASLNLDSVWEEEWQKN
                     LLDAAIERVKQQVTPKQFQIFDLSVLRELPVAEVKAILKVNAAQVYLARHRVGVLVKK
                     EAKRLEARAEKAVIKS"
     gene            3636..3833
                     /locus_tag="VIL39_07205"
     CDS             3636..3833
                     /locus_tag="VIL39_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481250.1"
                     /translation="MNPFFWERWLGWLRVPWPLRTRWRRYHVTPRTNPAMFRRELRRS
                     IRDELRASGLHTPSAARDPTH"
     gene            3839..>4142
                     /locus_tag="VIL39_07210"
     CDS             3839..>4142
                     /locus_tag="VIL39_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481251.1"
                     /translation="MPDPDPLPAIKTPWGSATPPSGASAGDVPPIPIPDHTLIRRIGK
                     GSYGEVWLARNALGAYRAVKIIDRRAFDDDRPFEREFAGMQRFEPVSRSHESQLNIL"
BASE COUNT          712 a         1291 c         1239 g          900 t
ORIGIN      
        1 gcgcaaacgc ccgccgcggc tttcatggtt ggccaatcgg agtcgctgcc catgatgatg
       61 ccgacgacgg gctttcctga gggcttgatg ctcatacggt gattgacaca gacgttgaca
      121 gcttaggcaa taaatgcgat ggcctcatgg cgggtattgt tcgctctggc agaccagaat
      181 tcaactatga aatggatcgc atctgaaagc cggaccacgg tgggccgctg gcggtccatt
      241 gccgaattcc tggctttgag gcgcaacacg agcctgctgc tggtggcgct cgtcctggcg
      301 ggcacgggcg aaaagctctg gctcggcttc gcgcccaagt acctccagac cctcggtgcg
      361 agcatcttcg tcattggctt gttcgacgcc ctgcaaacgc tgctcggcgc cctctatgcc
      421 taccccggcg gctggctgac cgatcgctgg gggcagcgcc ggtcgttgat gctcttcagc
      481 gtcctctcac tggcaggcta cacattggtc ctggtttggc gccattggct ggcgctgctg
      541 ctcggcacat tcctgtttct ggcttggagc gctttatcgt tgcccacgac gtttaccgtc
      601 gtcgccacct ccctcaaggc ccgccagcac accatgggca tcggcatcca atccatggtg
      661 cgccgcgtgc ccatgatgct cgggccgctg ggcggcgggt ggctgatcac ccggttcggc
      721 tggacccggg gcatacagtg tgccctgctg ctgtgcctct tgatgagcct gctgacgatg
      781 gtgttccaat ggttcatgct ggagccgggc caaggggagt ctcctcccac aaccgagtct
      841 ggcccgacga acttctttgc cgtcacgcgg tccttcaccc ccgcgctgcg ggagttgctg
      901 gtcagcgaca tcctgatccg cttctgcgag cgcattccct acgccttcgt cattttatgg
      961 gcgatgaacc atgccggcgt ggacgcgcag cagtttggag tgctggtcgc ctgcgaaatg
     1021 gtcaccgcca tggtctgcta cattcccgtc gcgcacctgg ccgacaagta tggccggcgc
     1081 cccttcgtgc tggtgacctt cgtcttcttc acgctcttcc ctttgacgtt gctctgggga
     1141 gacagctttg gctggctggt gctggccttt gtcgtccgcg gcttgaagga attcggtgag
     1201 ccgtcccgca aggcgctaat catcggggag gccgtcccgg cgctccgcgc gcgcacctac
     1261 ggcgcttact atctgattcg ggactgcgtg gtcaccagcg gctccttcct gggcgcgtgg
     1321 ctgtggagca tcggtccccg cgccaacttt atcggggccg ctgtctgcgg cgcgctggga
     1381 acggcctggt tctggtggtt tatctaccgc cggaagcccg ctgcaggatt gacaggaggc
     1441 cagaagggcg caaattagat cacagctcgt tcatgacaac agctaacaag attaccatcc
     1501 tgcgcatcct gctcgtcccg ttcttcgtgg tggaggtcct ctactatacc aaacagggcc
     1561 acgaggtgca ccgtctgctg gcgattgttt ccttcgccct cgcctccatt tgcgatggcg
     1621 tggacggcta tgtggcgcgg cggtttaatc agcgcagcga actcggggcg attctcgatc
     1681 cgctggcgga caagctgttg ctggtttccg gcattgtggt gctgagcttt gatcattcgc
     1741 cgtatctgga gagcataccg ctttggctca cgggcacgat tatcgggcgg gatgttttga
     1801 tattgatcgg catggtggtg attcatctga tggttggcaa agtcaaggtg cggccgcgga
     1861 tcatcggcaa gatcgcgacc gtgctgcaaa tggtcgtcgt gctctggatc atgttgaagt
     1921 ggggagagag ctggcttcgc gcgctgtgcc ttggtacggc catctgcacg ggcatttcgg
     1981 ggctgcttta cgtgtgggac ggaagccagc agttgagcgc gcacccgtcg tccagtccgg
     2041 cgtctaacgt tgaaagtcca aagcccaaag tctagatgcg ttccagacac ttcagaaacc
     2101 gataccgcgc gtaaaaccgc acgttataag cgggctggct catgatacgc caccggattt
     2161 gtgactggga ccactaagta gctctggttc aacattcgag cgcgggtgcg gcaagggtgt
     2221 cccacttgtg cgctcgcgtg gggatccacg ccccggcttt gagcgcgatt gggccgcaaa
     2281 agatcgcaag gaaagcaaag cggtaacggg aaaacccgct tcgactgctc gaagcgccga
     2341 ccacataggc ctcacgcata actccgttcg cagcgcttca ttgagacaaa catgtggttc
     2401 aatgccatca agtctccatt gcctttcgac gtcctgccgc ccggctccat tgacgatcgg
     2461 ccattgtcta ttcccagccc ggtcggcctc cattgccttt cggcgttctg ccccccggct
     2521 tccagcaatt ctcattatgg ccaaagccgg gggaaaacaa gtggtttcta cgcccaggct
     2581 ttggggtagc cttggggtag ccttgaggtg gctttggggt agccttgggg tagccttggg
     2641 gtgcctatca gttggctatc aacaggcctt ggggtggctc tgatgtcgca ttgggtggct
     2701 ttgtgcagcc ctctttctga tttcagcttt cagctttcag catttcagct tttgcccaat
     2761 tgtggcttta gggtggcttg cccgcccagt cacgaatcac gaatcacgaa tcacctccgg
     2821 ctttgggtgg ccttgcccgg cttttgctcg ttggatgttg gttgttcgat gttggatgtt
     2881 cggatgttcg gccaaccgaa gctttccgcc gcgatcaact taaggtaaag tagtttgaaa
     2941 gaaagccccg actcctgcga atatatgccc gtgcggaatg atgatgtcct agcctttggc
     3001 gccgaagacc cggccgccac gcggtccagt ctcgtgaagc gtctgcgcaa ttgggaagac
     3061 cacggcagtt ggcaggactt ctttgacacc tactggaagc tcatctacgc ggtcgccatc
     3121 aaggccggcc tgtccgaggc cgaagcccgc gacgtggtgc aggaaaccgt gctcgcggtg
     3181 gcgaaacaga tgcgcgaaaa cggctatgac cgcgccaaga gctcgttcaa gaattggctc
     3241 tgtctcatca cgcgccggcg catcattgat catttccgca aacgcaccga tccaaaggtt
     3301 cgcccgtcgc cgcgggctcg cgacggcacc agccgcaccg acaccatcaa ccgtgtgccc
     3361 gacccggcca gcctcaatct cgactctgtg tgggaggaag aatggcagaa gaacctcctc
     3421 gacgccgcca tcgagcgcgt caagcagcag gttacgccga agcagtttca gatctttgat
     3481 ctgtccgtcc tgcgtgaact gcccgtggct gaagtcaagg ccatcctcaa ggtcaatgcc
     3541 gcgcaggtct atctggcccg gcatcgggtc ggcgtgctgg ttaagaagga ggccaaacgc
     3601 ctcgaagcgc gggccgagaa agccgtgatc aaatcgtgaa cccctttttc tgggaacgct
     3661 ggttaggctg gctccgtgtg ccctggccgc tgcggacacg ctggcgacgc taccacgtaa
     3721 cgccgcgcac gaatcccgcc atgttccgcc gcgagctgcg ccgctccatc cgcgatgaac
     3781 tgcgcgcgtc cggcctgcat acccccagcg cggcacgcga ccccacgcat tgaccctcat
     3841 gcccgacccc gatccattgc ccgcgatcaa gaccccttgg ggctccgcca cgccaccctc
     3901 cggcgccagc gcgggcgacg tcccgcccat tcccatcccc gatcacacgc tcatccggcg
     3961 catcggcaag ggcagctacg gcgaggtctg gctcgcccgc aatgccctcg gcgcttaccg
     4021 cgcggtgaag atcattgacc ggcgcgcctt cgacgacgac cgcccgttcg agcgcgagtt
     4081 tgcgggcatg caacgcttcg agccggtttc ccgcagccac gagagccagc tcaacatcct
     4141 cc
//
LOCUS       DATCGS010000352         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_79775, whole genome shotgun sequence.
ACCESSION   DATCGS010000352 DATCGS010000000
VERSION     DATCGS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79775"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VIL39_07215"
     CDS             complement(<1..991)
                     /locus_tag="VIL39_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481252.1"
                     /translation="MEVAMKENMPMPGTQATIASAVFATSRRFKMNKSSLLPAGRFNR
                     TDRLCRRAKRTGGWSATGGFTLIELLVVIAIIAILAALLLPALANAKAKAVRVTCINN
                     EKQMVLAMSMYATDNTDHLAFPNWDGGVALTTPPSAGWLYTVINGAIPDTGPGGAYEN
                     NQINAYKGGLWFQYMPNPKSYLCPLDIKSPTWLARPGAANHRNNRMSSYIMNGAANNY
                     KNTTTGNPYWACKITEVWSTMCYLLWEPDENVNGSGNPGAFEFNDAANFPQISNGEGI
                     GRLHNKKGGAIVALAGHVQFISVEAFNKDSQAAGTPSAPGPGGRTLLWWAPGKA"
     gene            1204..2262
                     /locus_tag="VIL39_07220"
     CDS             1204..2262
                     /locus_tag="VIL39_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY5481253.1"
                     /translation="MIDTEQKLEAYLPGFRAATWVAVDTEADSLHAYPEKVCLIQIST
                     ASGDRLIDPLAGLNLDPLLEALSGRELIMHGADYDLRLLRKHQEFVPSAIFDTMLAAR
                     LIGLRQFGLSNLVEQYLGVKLEKGPQKANWAIRPLTERMERYARNDTLYLKPLADRLK
                     SELQAKGRLAWQQESCARLIQDSTRQHLPDLDAIWRIRGSHVLAPPALAVLRELWRWR
                     EKEAIAANKPPFFVMSHEALVNIAAAAALAQPIEPLLPRHLSDRRRGGLQKAVAHALQ
                     LSHDHHPKILRRVNRRPSEGERRRSIELEKCRDAQAAALGIDPTLIASRGVLSDLAYD
                     WEKHSPELMTWQRVLLKG"
BASE COUNT          454 a          702 c          681 g          495 t
ORIGIN      
        1 tggctttacc cggtgcccac catagtaagg tcctgccccc aggccccggg gcggaggggg
       61 tgccagccgc ctgggaatct ttgttgaagg cttcgacgct gatgaactgg acgtgccccg
      121 cgagagcaac aatcgctccg cctttcttat tgtgcagccg cccgatgcct tccccgttgg
      181 atatctgcgg aaagttggcg gcgtcattga attcgaaagc gcctgggttg cccgacccgt
      241 tcacgttttc atccggttcc cacaacaaat agcacatggt actccagacc tcggtgattt
      301 tgcacgccca gtaagggttg ccggtcgtag tattcttata gttgttcgcc gcgccattca
      361 ttatgtagct ggacattcgg ttgtttcgat ggttcgccgc gcccgggcgt gccagccagg
      421 tgggactctt tatgtccagc gggcagaggt acgacttggg gttgggcatg tattggaacc
      481 aaagccctcc cttgtaggca ttaatctggt tgttttcgta agcccccccc gggcccgtgt
      541 ccgggatcgc tccattaatg accgtataga gccagccggc cgaaggggga gttgtaagcg
      601 caaccccacc gtcccagttg ggaaaggcga gatgatccgt gttgtcggtg gcatacatgc
      661 tcatggctag taccatctgc ttttcgttat tgatgcaggt aacacgtact gccttcgcct
      721 ttgcgttggc gagtgccggc aaaagcagtg ctgcaagaat ggcgatgatg gcgatgacca
      781 ccagcagttc gattaaggtg aaacccccgg tggcagacca gccgccagtt cgtttggccc
      841 ttcggcagag cctatcagtc cggttaaaac ggccggcagg caatagggag gatttgttca
      901 ttttgaatct tctggaagtg gcgaagacag cggaagcaat cgtcgcttga gttccaggca
      961 tcggcatatt ctccttcata gcaacctcca tacggtttgg attctgaacc tgtcttcaga
     1021 tcaataatcg cccgtttatg ccggtcacaa gctgcaccag cgcttcgcat gttttaaagt
     1081 aagatcattc ggcctgagat tgcaagcaca aacttcgcgt tgatctggcc tcgatgctat
     1141 ccgaactaac cctttcgacg atttagctat ggcccttttt cgcggcgcaa atatagttac
     1201 caggtgattg atacggaaca gaagcttgaa gcctacctgc ccgggttccg ggcggcgacg
     1261 tgggtggctg tggatacgga ggcggacagc ttgcacgcgt acccggagaa agtctgcttg
     1321 attcaaatca gcacggcttc cggcgacagg ctcattgacc cgctcgcggg cctcaatctc
     1381 gacccgctgc tcgaggcgct gtccggccgc gagctgatca tgcatggggc cgattacgac
     1441 ttgcgcctgt tgcgcaaaca ccaggaattt gtgcccagcg ccatcttcga cacgatgctc
     1501 gccgcgcggc tgatcgggct gcgccagttc ggtctgagca acctggtcga gcagtatctc
     1561 ggcgtcaaac tggagaaggg accgcagaaa gccaattggg ccatacgccc gctgaccgag
     1621 cggatggagc gttacgcccg caacgatacc ctttatctca agccgctcgc cgaccggctc
     1681 aagtcggaac tgcaggccaa aggtcggctg gcctggcagc aggaatcctg cgctcggctc
     1741 atccaggact cgacccggca acacctgccc gatctggacg ccatctggcg gatcagaggc
     1801 agccatgtgc tcgcgccgcc ggccctggcc gtcttgcgcg agctgtggcg ctggcgggaa
     1861 aaggaggcga ttgccgccaa caagcccccg tttttcgtca tgagccatga ggcgctggtc
     1921 aacattgccg cggcggccgc cctcgcccag cccatcgagc cgctgctgcc caggcatctt
     1981 tccgaccggc ggcgtggcgg cttgcagaaa gcggtcgcgc acgcgctgca actttcccac
     2041 gaccaccacc ccaaaatcct ccgccgcgtc aaccgccggc ccagcgaagg ggaaaggcgc
     2101 cggtctattg agctagagaa gtgccgcgac gcgcaggctg ccgcgcttgg cattgacccc
     2161 accttgattg ccagccgcgg cgtgctctcc gacctcgctt acgattggga aaagcactcc
     2221 cccgagttga tgacgtggca gcgcgtattg cttaaggggt gacatcgcgc atcggttctc
     2281 caatcaggtt cctcgacggt tcccattgag ccaaggcgat aaacccgaag gc
//
LOCUS       DATCGS010000353         2429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_327973, whole genome shotgun sequence.
ACCESSION   DATCGS010000353 DATCGS010000000
VERSION     DATCGS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327973"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIL39_07225"
     CDS             complement(<1..202)
                     /locus_tag="VIL39_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481254.1"
                     /translation="MKHITSIPRRVFLKSLGLAVPVAAASPDVAFSQTQNSSTAPGSL
                     KQAAAKELAADLVVIGGSVGGCA"
     gene            complement(338..814)
                     /locus_tag="VIL39_07230"
     CDS             complement(338..814)
                     /locus_tag="VIL39_07230"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HEY5481255.1"
                     /translation="MNLEPKPGQWMEPEDFNRVVRLTPLVAIDLIVRSPDRRVLVGRR
                     TYEPAKGCLFVPGGRITKNETLAAAFRRVSLAELGVEKKMEEAPFLGVYEHFYSTNNL
                     EQPGFGTHYVLLTYEVTSPVPEASLPKDQHAEYFWLTEADILTCPEVHEHTKAYFR"
     gene            937..2241
                     /locus_tag="VIL39_07235"
     CDS             937..2241
                     /locus_tag="VIL39_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481256.1"
                     /translation="MIHSISRRDFLKRSSVLTAGLATLGSGASLRAAKSPNEKVVIAI
                     VGCNARGMAHIAGYLPLPNAEIGYICDVDSRAVEKGIAAVARKQQRKPQGVKDFRRIL
                     DNPDVDAVSIATPDHWHAPATILACAAGKHVYVEKPGSHNLRESELMVAAARKYKRVV
                     QMGNQRRSWPWVIESIGALHAGELGWVCFARGWYTNHRTSIGHGKPAPVPEWLDYSLW
                     QGPAPERPFQDNIIHYNWHWFWNWGTGELGNNGVHALDLARWGLQVELPGRVTCGGNR
                     YFYQDDWETPDTMMATFDFGDKGITWEGQSCAPRGFEGSSFGVNFYGEKGCMAMAGSN
                     STIYDLNNKVVREIKGKSEGLFDFDSIHFGNFLDGIREGKPLKSEIEEGQKSTVLCHL
                     GNIAWRTGHTINFDPKERKIVGDQDASALIGRTYRKGWEPKV"
BASE COUNT          494 a          722 c          748 g          465 t
ORIGIN      
        1 ccgcgcagcc gccgacgctt ccgccgatga cgacaaggtc ggcagccaat tcctttgctg
       61 cggcttgctt caggctgcca ggcgcagtcg aggaattctg ggtctggctg aaggcaacgt
      121 ccggactggc tgcagccacg ggaacggcta ggccaaggct cttgaggaag actcgacgtg
      181 gaattgaggt aatgtgtttc acgggccaaa actaccaacc cgaatggaac tgacaagcaa
      241 agccgcagtt cgatctcgcc gcgtgccctc acgcggcgac actcacttat cggttacctg
      301 cggaaccaag cacttgcgtg ctccaacggc ggaagaccta ccggaaatag gctttggtat
      361 gctcgtgaac ttccggacaa gtcaggatat cagcctccgt cagccagaag tattctgcat
      421 gctggtcctt tggcagcgac gcctccggca cgggcgacgt cacctcgtag gtgagcaaga
      481 cgtaatgcgt gccgaagccc ggttgctcaa ggttattggt gctatagaag tgctcataca
      541 cgcctagaaa tggggcttcc tccatcttct tttccacgcc gagctcggcc agggatactc
      601 ttctgaaggc ggctgccaac gtctcgttct tggtgatgcg gccgccaggg acaaagagac
      661 accccttggc aggctcgtag gtgcgacggc ccaccaggac ccgcctatcg ggcgaccgaa
      721 caatcaggtc aatcgcgact agcggcgtca gcctgacaac gcggttgaag tcttcgggct
      781 ccatccattg cccgggtttt ggttcgagat tcattgaggc gagtgatcgt atcagactgc
      841 cccacgacgt ccagcacggg agcggggtcc tcgtatctga cgcgctgtgg caaagttttc
      901 ttgctagaac cccctcaaag gaatctcatg gaggccatga ttcactcaat cagccgccgg
      961 gactttctaa agcgaagctc ggttctaact gcggggctcg ccactttggg cagcggcgcc
     1021 tcgctgcgcg cggccaagag cccgaacgag aaagtcgtga ttgccatcgt cggttgcaat
     1081 gcccgcggga tggcccacat cgccggttat ctccctctgc ccaacgccga gattggctac
     1141 atctgtgacg tggacagccg ggcggtcgag aaaggcatcg ctgccgtcgc caggaagcag
     1201 caacggaagc cgcagggcgt taaggacttc cgccgtatcc tggataatcc cgacgtggac
     1261 gctgtttcca ttgccacgcc cgatcactgg catgcgcctg ccaccattct ggcctgcgcg
     1321 gccggcaagc acgtctatgt cgagaagccc ggcagtcaca acctgcgcga gagcgagctc
     1381 atggtcgccg ccgcgcggaa atacaagcgg gtcgtgcaaa tgggcaacca gcgccgcagt
     1441 tggccgtggg tcatcgagtc cattggcgct ctgcacgcgg gcgagcttgg ctgggtgtgc
     1501 tttgctcgcg ggtggtacac caaccaccgc acgtcaatcg gccacggtaa accggccccg
     1561 gtgccggagt ggctcgatta ttccctgtgg cagggacccg cgccggaacg gccattccaa
     1621 gacaatatca tccattacaa ctggcattgg ttctggaact gggggaccgg cgaactcggc
     1681 aacaacggcg tccatgcgct cgatctcgcc cgctgggggc tgcaggtgga acttccgggg
     1741 cgcgtgacct gtgggggcaa ccgttacttc taccaggacg actgggaaac cccggacaca
     1801 atgatggcca ccttcgattt cggtgacaag ggcatcactt gggaaggcca gagctgcgcc
     1861 ccgcgcggct tcgaaggctc aagctttggg gtgaatttct atggtgagaa aggctgcatg
     1921 gcaatggcgg ggagcaacag caccatctat gatctgaaca acaaggtcgt ccgcgaaatc
     1981 aagggcaaga gcgagggcct gtttgacttt gactcgatcc atttcggcaa cttcctggac
     2041 ggcatccgcg agggcaagcc gctgaaatcc gagatcgaag agggccagaa gagcaccgtg
     2101 ctctgtcact tgggcaacat cgcctggcgc accgggcaca cgattaactt cgaccctaag
     2161 gagcgcaaga ttgtgggcga ccaggacgct tccgccctga ttggccgcac gtaccgcaaa
     2221 ggctgggagc cgaaagtcta gcgggtgaaa cgtggtgcgg gattcgtgat gcgtgaaggg
     2281 ggacgcctca ggggaactgg gctttgtctt cgtacggctc cagccgggcg atcgccgcca
     2341 tgagttcatt ggcgacctgt tggtagattt ccttgaggcg cggcttcgag cacaccttcg
     2401 cctccgcgcg tagctgttcg aacctcatc
//
LOCUS       DATCGS010000354         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_225713, whole genome shotgun sequence.
ACCESSION   DATCGS010000354 DATCGS010000000
VERSION     DATCGS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225713"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VIL39_07240"
     CDS             <1..1266
                     /locus_tag="VIL39_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5481257.1"
                     /translation="LVLRRQLFSWQQIESADYPTYIANLRDVGCPEQTIRDIIIADVN
                     QLYSRRRATELTTAEQQWWRSEPDTNIVRAAGQKALALENERRALLTRLLGITWETGD
                     LVSLPRPSRPGVVLDGAVLGSLPDETKQAIQDVSQRSQDRMQAYLDAQRSAGKEPDPV
                     ELAKLRQQTRDDLARLLSPPALEEFLLRYSQNANDLRTEFGQLRFFDPTPDEFRAVFQ
                     ATDTLDQRIQLLADATDPGSVAARKALEAQRDNAIKAALGSKRYEEYHLLHDPVYRDA
                     MATADEAGTPEAAGTFYAINLAALAEQNRIRGDTNLTADQKSIELKRLELEQLQANTV
                     AKGRDLPPEPPPLPPVPPQRKVIIMGPGDSAATIATMYGLPVSAIKDANPNVDFNKLK
                     QGDAIIVPKSPFSPLTPPFRPPFPPPLPP"
     gene            complement(1416..2402)
                     /locus_tag="VIL39_07245"
     CDS             complement(1416..2402)
                     /locus_tag="VIL39_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5481258.1"
                     /translation="MKLTALKSAILLFLISSVAAPAATEEKLNQRFAAQQGGTVVVEV
                     DFGSIDVSTNATSEVVVDVWRKIGWRKKADEEAFLRDNPVKFTQEGNTVTIRCPGTSK
                     WSWSLIGRNENDAKYTISVPAKFNAQIKTGGGGVRLVDLTGEVKAHTGGGGLSFARLH
                     GALNGDTGGGGVTANDCEGALNFRSGGGGIEVSGGSGSLAGTTGGGSVSVRKFQGAAH
                     VTTGGGGLIIENVDGGVDASTGGGSIRAVLPSELADAVKLSTGGGGISVGVPATAAFN
                     LDAKTSGGNVSTDLPVTMVGKMEEGRLQGPVNGGGKAMQLRSGGGSIHLKKL"
     gene            complement(2537..>2829)
                     /locus_tag="VIL39_07250"
     CDS             complement(2537..>2829)
                     /locus_tag="VIL39_07250"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEY5481259.1"
                     /translation="DLTTLRKLMLEVADEHPKALKDPKPELFFVGFGASSLDFELGVW
                     ASDATTSPRRFRSDMFFAIEQKLRENDIEIPFPQQVLHVRPAAGDALPAAHP"
BASE COUNT          544 a          953 c          828 g          504 t
ORIGIN      
        1 cttgttctgc gccggcagct tttctcctgg cagcagatcg agtcggcgga ttaccccacc
       61 tacatcgcca acctgcgtga tgttggctgc ccggagcaga cgattcgcga catcatcatt
      121 gcggacgtta atcagcttta ctcgcgaaga cgggccacgg agctgaccac ggcggagcag
      181 caatggtggc gcagtgagcc ggacaccaat atcgttcgcg ctgccggcca aaaggctctt
      241 gcactggaga atgaacggcg cgccctgcta acgcggctgc tggggataac ttgggagacg
      301 ggcgacctgg tgagcctccc gcggccatcg cgaccgggcg tggtgctcga cggcgccgtg
      361 ctgggctcgc tgccggacga aaccaagcag gccatccagg atgtcagcca gcgctcccag
      421 gaccgcatgc aggcttacct ggacgcgcag cgcagtgccg ggaaggagcc cgacccggtt
      481 gaactggcca agctccggca gcaaacccgc gacgacctgg cccgtttgct cagtccgccc
      541 gcactcgaag aattcctgct gcgctactcg cagaacgcca acgatctgcg cacggagttc
      601 ggccaactcc gattcttcga ccccacgccc gacgagtttc gtgcagtgtt ccaggccacg
      661 gacaccttgg accagcggat ccagttgctg gcggatgcga ccgacccggg cagcgtcgca
      721 gcgcgcaagg cgctggaagc ccagcgcgac aacgcgatta aggctgcgct cggctccaag
      781 cgttacgagg agtatcacct gctccacgat ccggtttacc gggacgccat ggcgactgcc
      841 gacgaggccg gcacgcccga ggctgcgggg acattctacg ccatcaacct ggccgccctg
      901 gccgaacaaa accgcatacg cggcgacaca aatctgaccg ccgaccagaa gagcatcgag
      961 ttaaagcggc ttgagctgga acagcttcag gccaacacgg tggcgaaggg acgagacttg
     1021 ccgccggaac cgccgcccct cccaccggtg cctccgcaga gaaaagtgat tattatgggc
     1081 ccgggcgaca gcgcagccac catcgcgacg atgtacggcc tgccggtcag cgccatcaag
     1141 gatgccaacc cgaacgtgga cttcaacaag ctcaaacagg gggatgccat catcgtcccg
     1201 aaatctccat tcagtcccct tacacccccg tttaggcccc cattcccacc ccctttgcca
     1261 ccgtgagtgt gtaatgccag cccccgccga cagagcgctc caaatccgct gcggctgtta
     1321 gcggttccat gaaagccgcc ccggcatact ccgggcttgt tctcgccggg cgggcggata
     1381 ggtttcctgg cgtgagtaag gcagcgagat tctcgctaaa gcttcttcaa atggatgctt
     1441 cctccgccgc tgcgcaactg catggctttg ccgcccccat tgaccgggcc ttggagccgg
     1501 ccttcctcca tcttgcccac catggtcact ggcaggtctg tgctgacgtt gcctcccgag
     1561 gtcttcgcgt ccaggttaaa ggccgcggtc gcgggtacgc ccacgctgat gccaccgccc
     1621 ccggtcgaaa gcttcacggc gtcggccaat tcagagggca gcaccgccct gatggaaccg
     1681 ccgccggtcg aagcgtcaac cccgccgtcc acattctcga tgatgagacc accaccgccg
     1741 gtggtgacgt gcgccgcccc ctgaaacttc ctgacgctca ccgagccgcc tcccgttgtg
     1801 ccggcgaggg agccggagcc gccggagact tcgataccgc cgccacccga gcggaagttg
     1861 agggcgcctt cgcaatcgtt ggcggtgacg cctccgcccc cggtgtcacc gttaagcgca
     1921 ccgtgcagac gcgcgaaact cagtccccct ccgccggtgt gggccttcac ctcgcccgtg
     1981 agatcaacca ggcgcacccc accgccgccg gtcttgatct gggcgttgaa cttcgccggc
     2041 acgctgatcg tgtacttggc atcgttttca ttacgcccga tcagggacca agaccacttg
     2101 gatgtgccgg ggcaccgaat cgtcaccgta ttcccctcct gagtgaactt gacgggattg
     2161 tcgcgcagga aagcctcctc gtcagccttc ttcctccagc caatcttgcg ccagacatcc
     2221 acgacgactt cgctggtggc gttggtgctg acgtcaattg aaccgaaatc cacctccaca
     2281 acgaccgtgc cgccttgctg cgcggcgaag cgctggttga gtttttcctc cgtcgcagcc
     2341 ggggcggcga cagagctgat caggaaaagg agtatggctg acttgagtgc ggttagtttc
     2401 ataacaatca atcttctcgc tgcggccatg ctcggtcggt taatgaccaa gctgcccggc
     2461 cacagcatca tctgactgtc ttcttaagga aatcccgccc aaagttgcaa tcaaattggg
     2521 aaatcgccca aaggactcag gggtgcgccg ccggcaaagc atcccctgct gcggggcgca
     2581 catgcaagac ttgctgcggg aaggggattt cgatgtcgtt ttcgcgcagc ttctgctcga
     2641 ttgcgaagaa catgtcactc ctgaaacgcc gcggcgaagt ggtcgcgtcg ctcgcccaga
     2701 cgccgagctc gaagtccaaa gaactcgcgc caaagccaac aaagaacagc tcgggcttgg
     2761 ggtccttcag cgccttggga tgctcgtcgg cgacttccaa catgagcttg cgcagcgtgg
     2821 tcaggtccg
//
LOCUS       DATCGS010000355         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_723828, whole genome shotgun sequence.
ACCESSION   DATCGS010000355 DATCGS010000000
VERSION     DATCGS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723828"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2398
                     /gene="rnr"
                     /locus_tag="VIL39_07255"
     CDS             95..2398
                     /gene="rnr"
                     /locus_tag="VIL39_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY5481260.1"
                     /translation="MEDKILGLLKRPNYTPQNAAELLAQLGLRHSQQRELEHVLAQME
                     RSGQIARIKQGNRYALPLTADLVPGRIRMNRAGVGFLEADDPKITTIRIPQDATSTAM
                     HGDRVLVRRDVLPRVPRRNERDEATGRVIRVLERARTRLVGTLQRGKQFLYVIPDDPR
                     ITHDIYVTPPRDTGRPAQMGDKVVVDLREWTLPRSNPEGEIIEVLGPPDAEGVDMLSV
                     IRQYNLVLHFPKRVLHEAELVGKEVKPAERAGRTDCRSHQVVTIDPDDAKDFDDAICL
                     QRSGPDHWKLWVHIADVSHYVKPGTALDEEAARRGNSTYMVDRVVPMLPEALSNELCS
                     LKPQVERLTKCVEFLLSPEGQVLKTQFYPAVIRSQRRYNYKEVLALLQRPPQDPIEQM
                     LHDANALTQKIRRARFKAGSLNLDFPETKIRLDERGRVLRMEKMENDVSHQLVEECML
                     LANEAVASRLMAMNRPALYRVHEPPEDRRLQEYREEVLSHHIQCGNLSNRQEVQKLLE
                     KLNGLAIGQALKIGFLKSLMRARYAVEPLGHYGLAKKKYTHFTSPIRRYADLVVHRAL
                     FQQPGQRVALRPLKEVAQHISETERNSDDAERDSRDLKMFAFLKAQLKSAHPTRYPAL
                     VTDVRNFGFFVDVTGLGMSGLVPLSGVSDDFYQFDPTRGQLVGRRTRRVFKLGDNVEV
                     QIAKVDTFKKQVDFKLAGMTPDRARRDVRPERQSQRPRAEKETRSAPPRSGSLSKAQG
                     QRWQAPRRSGGRRPQRGPGGPQGRAPR"
BASE COUNT          470 a          727 c          805 g          403 t
ORIGIN      
        1 tgagctgagt gcctcgaggc gattagtctc gacgcctggt gccggcgtcg tgcatattag
       61 caggcaatac ctcacttggg tcaatttacc ttatatggaa gacaagattt taggcctgct
      121 taaaaggccg aattacacac cacagaatgc cgcggagttg ctggcgcaac tcgggttgcg
      181 ccacagccag caacgggaac tggagcacgt gctggcgcag atggagcgca gcggccaaat
      241 cgcccgcatc aagcaaggca accgctacgc gctgccgctc accgccgacc tggtgccggg
      301 ccgtatccgc atgaaccgcg cgggggtggg tttcctcgag gcggacgatc caaagatcac
      361 gaccatccga attccgcagg atgccacttc gaccgcgatg cacggcgacc gtgtgttggt
      421 gcggcgtgat gttctcccgc gtgtcccgcg gcggaatgag cgggacgagg ccaccggccg
      481 tgtcatccgc gttttggagc gggcgcggac gcgattggtc ggcacgctgc aacggggcaa
      541 gcagtttctc tatgtcatcc cggacgaccc gcgcatcacc catgacatct acgtgacgcc
      601 gccgcgcgac accgggcgcc cggcgcaaat gggagacaag gtggttgtgg atctgcgcga
      661 gtggacgttg cctcgctcga atcccgaggg cgagatcatc gaagtcctcg gcccgccgga
      721 cgccgaaggg gtggatatgc tttcggtcat ccgccaatac aatctggtgc tgcacttccc
      781 gaagcgagtg ctgcacgaag cggagttggt gggcaaggaa gtcaagccgg cggaaagggc
      841 cgggcgcacg gattgccgca gccatcaggt tgtcaccatt gatcccgatg atgccaagga
      901 tttcgacgac gcgatctgcc tgcagcggtc cggcccggac cattggaagc tgtgggtcca
      961 catcgccgac gtgtcgcatt acgtgaagcc cggcaccgcg ctcgacgagg aggcggcccg
     1021 gcgggggaac tcgacctaca tggtggatcg ggtggtgccg atgctgcccg aggccttgag
     1081 caatgagctg tgctcgctca agccgcaggt ggagcggctg accaaatgcg tcgagttttt
     1141 gctgtcgccc gagggccagg tcctgaagac acagttctat cccgcagtga tccgctcgca
     1201 gcgccgctac aattacaagg aagtgctggc cctgctgcag cgcccgccac aggatccgat
     1261 cgagcaaatg ctccacgacg ccaatgcgct gacccagaaa atccggcgcg cgcgattcaa
     1321 ggccgggtcg ctcaacctcg acttccccga gaccaagatc cgcctcgatg agcgcgggcg
     1381 ggtattgcgc atggagaaga tggagaacga cgtttcccac cagctcgtcg aagagtgcat
     1441 gctgctggcc aacgaggccg tggccagccg gctgatggct atgaatcggc cagcgctcta
     1501 tcgcgtccac gagccgccgg aggaccggcg gctccaggag tatcgcgagg aggtgctcag
     1561 ccatcacatc cagtgcggca acctgtcgaa ccgtcaggaa gtgcagaagc tgctggagaa
     1621 gctcaacggg ttggcgatcg gccaggcgct gaagatcgga tttctgaagt cgctcatgcg
     1681 cgcgcgctac gcggtcgagc cgctcggcca ttacgggctg gcgaagaaga aatacacgca
     1741 tttcacctca ccgatccggc gctatgcgga cctggtggtg catcgcgccc tgttccagca
     1801 gccgggccag cgggtggcgc tgcggcctct caaggaggtc gcgcaacaca tctccgagac
     1861 cgagcgcaac tccgatgacg cggagcgcga cagccgggat ctgaagatgt tcgcgttcct
     1921 gaaggcccag cttaagtccg cgcatccgac ccggtatcca gcgctggtta ccgatgtgcg
     1981 gaacttcgga ttctttgttg atgtgacggg gttggggatg agcgggctgg tgccgctctc
     2041 cggggtgagc gacgacttct atcagtttga tccgacgcgc ggccagcttg tgggccggcg
     2101 cacccggcgc gtgtttaagc tgggcgacaa cgtggaggtg cagatcgcaa aggtggacac
     2161 cttcaagaag caagtggact tcaagctggc cggcatgaca cccgaccgcg cgagacgaga
     2221 tgtgcggccc gagaggcagt cacagcggcc gcgtgcagag aaggaaacgc ggtctgctcc
     2281 ccctcgctct ggctcgcttt ccaaggcaca gggacagagg tggcaggcgc ctcgacgaag
     2341 cggagggcgg cgcccacaga gaggcccggg cggtcctcag ggcagggcgc cgaggtgacc
     2401 agtta
//
LOCUS       DATCGS010000356         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_624834, whole genome shotgun sequence.
ACCESSION   DATCGS010000356 DATCGS010000000
VERSION     DATCGS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624834"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VIL39_07260"
     CDS             <1..834
                     /locus_tag="VIL39_07260"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEY5481261.1"
                     /translation="RPNVIVRLCSIECNFREPLDHPSNAAFLADLKTWSRMCSRLYVW
                     DYVTDFSNYVLPHPNWFVLGPNVRLFRDYGVKGVFEEGAYAGPGEEMAELRAWVLAQL
                     LWNPQQDDRALIKDFLHGYYGTRAGEIIERYLELMHNSSAGFYLGCYLRKDAPHLRFQ
                     PLAAAERLWQQAEEAASREADPEKLIRVRIGHLPVRYACLGRWVSLRRECREQNGTWP
                     WPESRKAVADAFREVCQGVPGKDWTTVQVLSEGGRKVDDFLKEFAQDPPDANGPPTPK
                     R"
     gene            1011..1925
                     /locus_tag="VIL39_07265"
     CDS             1011..1925
                     /locus_tag="VIL39_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5481262.1"
                     /translation="MVLAKPWKADAIIRLVLSVMVCVYAGSVLASAGHYVNAGGKASP
                     WLFFPLVAVALICLVVALILIGKPWPTEAFGSRILALLVCSYGGLFLGAWAQRLTGEG
                     ASEASIWRMVLATLSFQGAGLFLIARFLREQQSSWTEGFGLLNRPRQAMLLGLLAALI
                     FLPIGWGLQQASAFVMTHLPHFKIEPKEQLPVHALRVSMSWGGRLMLGAAAVLLAPVT
                     EEVLFRGILYPAIKQFGYPRLALWITSLLFAAVHMNTVTFVPLAALALVLTALYEWTD
                     NLLAPVMAHVLFNALNFAMLLVQQVGWL"
     gene            1922..>2647
                     /locus_tag="VIL39_07270"
     CDS             1922..>2647
                     /locus_tag="VIL39_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5481263.1"
                     /translation="MNEFDLIHRLTHTLPTNPSVVAGAGDDCAVLDVGIPDRHLLFKT
                     DAVVEGVHFTTQTAPEKIGHKALGRCLSDIAAMAGTPTAALVTIGLPPKFSPEFVEAI
                     YAGLNTLARRHQVAVVGGETTANPGGIFISVALLGWVPRGKGVLRSGAEVGDAIFVTG
                     ELGGSLAGKHLDFEPRLAEARWLAQEFSLHAMLDVSDGLAGDLRHILTASRVGAELLA
                     TAIPISREARRAAKGRTAGVRPSP"
BASE COUNT          408 a          794 c          916 g          529 t
ORIGIN      
        1 cggccgaacg tcatcgtgcg gctgtgttcg attgagtgca atttccgcga gccgctggac
       61 catccgtcca atgccgcctt cctcgctgat ctgaagacat ggtccaggat gtgttcccgg
      121 ctttacgtgt gggactacgt caccgatttc agcaactacg tgctgccgca tccgaactgg
      181 ttcgtgctgg ggccgaacgt ccgcctgttc cgggattacg gtgtgaaggg ggtgtttgag
      241 gaaggcgctt atgccggccc gggggaggag atggccgaac tgcgggcgtg ggtgttggcg
      301 caactgctct ggaacccgca acaggatgac cgggcgctga ttaaggattt cctgcacggc
      361 tactatggca cccgggcagg ggagatcatc gagcgttacc tggagttgat gcacaactca
      421 tctgcgggtt tctacctggg ttgttacctg cgcaaagacg cgccgcacct gcgtttccag
      481 ccgctggccg ccgcggaacg cctttggcag caggccgagg aggcggccag ccgcgaggcc
      541 gaccctgaga agctgatccg cgtccgcatc gggcacttgc cggtgcgcta cgcctgcctg
      601 ggccgctggg tgtcgctacg ccgggaatgc agggagcaaa acgggacctg gccgtggccg
      661 gaatcacgca aggccgtagc tgatgccttt cgcgaggtgt gccagggtgt tccggggaag
      721 gactggacga cggtgcaggt gctcagtgaa ggggggcgga aggttgacga tttcctgaag
      781 gagtttgccc aggatccacc tgacgcgaat ggcccgccga cgccgaagcg gtagatcaat
      841 ccgccaagcc cgcggccctg aaacccgaag gccgaaatcc gaaccgacag cagcccatgc
      901 tgaccagcgg ccctccggac tgagccttct tcgggtttct ggtttcgggc ttccttcggt
      961 cttcggtttt ttccatcgtc accccgccgc ggctgggcta gccttggcgc atggtattgg
     1021 cgaaaccgtg gaaggcggat gcgatcatac gcttggtgtt gagcgtaatg gtctgcgtct
     1081 atgccggctc ggtgctcgcg agcgcggggc actacgtcaa cgccgggggc aaggccagcc
     1141 cgtggctatt cttcccgctg gtggccgttg ccctgatctg cctggtcgtg gcgctgatcc
     1201 tgatcggcaa gccctggccg acggaggctt tcgggagccg gatactggcg ctgctggttt
     1261 gttcctatgg cggattgttc ctgggcgcat gggcgcagcg attgaccggg gagggagcgt
     1321 cggaggcttc catttggcgg atggtcctgg ccacgctgag ctttcagggc gcgggcttat
     1381 ttctgattgc ccgtttcttg cgcgagcagc agagcagttg gacggagggc ttcggattgt
     1441 tgaaccgccc gcgccaggcc atgctgctgg ggttgctggc ggcgctgatc ttcctgccga
     1501 tcggctgggg actccagcag gcttccgcgt tcgtgatgac gcacctgccg cacttcaaga
     1561 ttgaacccaa agaacaactc cccgttcatg cgctgcgggt ttccatgtca tgggggggac
     1621 ggctgatgct gggggcggcc gcggtgctgc tggcgccggt tacggaggaa gtgctgtttc
     1681 gcggcattct ctaccccgcg atcaagcaat tcgggtatcc gcggctggct ttatggatca
     1741 cctcgctgct gtttgcggcg gttcacatga acacggtcac gtttgtgccg ctggcggcgc
     1801 tggctctggt tttgacggcg ctctatgagt ggacggacaa cctgttggcc cccgtcatgg
     1861 cgcacgtgct gttcaacgcg ctgaatttcg ccatgctgct cgtgcagcaa gtcggctggc
     1921 tatgaacgag ttcgacttga tccaccgcct gacgcacacg ctgccgacca acccgtcggt
     1981 cgtcgcaggc gcgggcgatg attgcgcggt gctggatgtc ggcattcccg accggcacct
     2041 cctatttaaa accgatgccg tggtggaggg cgttcacttc acgacccaga cggcgccgga
     2101 gaagatcggc cacaaagccc tgggccggtg tttgagtgat attgcggcca tggcgggaac
     2161 gccgaccgcg gcgctggtga cgattggcct gccgcctaag ttcagtcctg agtttgtgga
     2221 ggcaatctac gctggcctga atacgctggc gcgacggcac caggtggccg ttgtcggggg
     2281 cgaaaccacg gccaaccccg gcggcatctt catctcagtc gcgctgctcg gctgggtgcc
     2341 gcgcgggaag ggtgtgctgc gttcgggcgc cgaggtgggc gacgccatct ttgtcaccgg
     2401 cgaactgggc ggctcgctag ccgggaaaca tctcgacttc gagccgcgcc tcgccgaggc
     2461 gcgctggctc gcgcaagagt tttcgctgca cgcgatgctg gacgtaagcg acgggctggc
     2521 gggcgacctg cggcatatcc tgaccgccag ccgcgtgggc gcggagctgc tggcgaccgc
     2581 gattcccatc agccgcgagg cgcgccgcgc cgccaaaggc cggaccgccg gggtgcgccc
     2641 gtccccg
//
LOCUS       DATCGS010000357         6460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_575139, whole genome shotgun sequence.
ACCESSION   DATCGS010000357 DATCGS010000000
VERSION     DATCGS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575139"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VIL39_07275"
     CDS             <1..250
                     /locus_tag="VIL39_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481264.1"
                     /translation="PLWSLWRSENNPRAGATSQSLLWNLYRREATPAARKCSLLFGLF
                     QYQSSSEGKDMRLFYIPLGGARPPAQSGANSAPAKTAQ"
     gene            326..1063
                     /locus_tag="VIL39_07280"
     CDS             326..1063
                     /locus_tag="VIL39_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5481265.1"
                     /translation="MFQNIGEMVLLFWRTLVALPLAWRQRQKVFDQFFEIGNASLLMV
                     CILSFFIGGVIALQTGPLLVERGLGSYVGGIVGWTIAKELAPVMMGILIAGRIGSAMA
                     AEIGSMRVYQEIDALRTMNISPIHYLVLPRLVAIAAALPLLVTFGILVGWLGGSVVCV
                     ANDRIAVSFEAFFSSLRDAVDLKDVANGVFKSFVFALTIGVVSCHQGLITRGGPRGIG
                     RSVTKAVVNSIVLIVILDYLLTRILLN"
     gene            1063..1860
                     /locus_tag="VIL39_07285"
     CDS             1063..1860
                     /locus_tag="VIL39_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481266.1"
                     /translation="MNGSSTNHHGVGIQVRGLRKSFDGQEVLRGLDFEVQPGEIFVIM
                     GPSGSGKTVLLKHLIGLEEPDAGEILIEGQPIQTPEVMSQYRMAMVFQSGALLNSLTV
                     GENVGLYLAEHRLQPPAEIARIVAEKLEVVGLKGTEEKMPSELSGGMKKRVAIARALV
                     IDPQLILYDEPTSELDPLSAVMIAEEILHLKDRIGVTSLVVSHDRDLAFGVADRIAVI
                     NQGRILTIGTPDEVKQSPDSLVQQFLHADFKRGRAPLEPSKVYEKQS"
     gene            1844..2827
                     /locus_tag="VIL39_07290"
     CDS             1844..2827
                     /locus_tag="VIL39_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5481267.1"
                     /translation="MKNSLETRIGLFVALAVLAAAIVLERVGSFEMFERGKHVKALFA
                     NVQDLKVGDRVKMAGVEIGRVQGIQITNNRVRVMMKVRPTAEVKTDSIATIKFTGLLG
                     QNFVSIDFGTPQGEPLKEDQYVATAEQADLSAMMQKIDNVASGVENLTKSFTGFKIDT
                     LVGPLRDFISANKDPLTMTISNLQTLSSNISAQVEQGHGTLGKLIQDETLYNTALSTV
                     SNLQDTAIEIKATVADARKLVDQANAGRGTVGKLLTDDSLFRETTNAMTNLKEILQKV
                     NQGQGTVGKLVNDQEFYKNAKLTLQKLDQATEGLEDQGPLSVLGLAVGKLF"
     gene            2870..3547
                     /locus_tag="VIL39_07295"
     CDS             2870..3547
                     /locus_tag="VIL39_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481268.1"
                     /translation="MSSDYDATEFVDGDFQAHKVPASATSAGGSQSQRAPTREEVDSR
                     VAEAQQKLAELKREQEELERQRAALEETRRRQMEFQTGRQETLQNLTRGVGLLEEAEF
                     SARRDAEQMAKALVNLRDALAKLAAIHEEGWTKDNFSMELTRALTALENARMEWNAAR
                     LKFPILSNPSQDGSAGAADVAAPRTSLLGTHSFGELCRIGFALTWPLAAVALVALAAL
                     LVFLARH"
     gene            3561..4094
                     /locus_tag="VIL39_07300"
     CDS             3561..4094
                     /locus_tag="VIL39_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481269.1"
                     /translation="MAWFTKKPDPISDRARTLNDEIAKLESRIQQLDAQVQRGQTQPR
                     LRSTALPHSSTISHATPTSAPPPEPVTHEPIFEEVGQDRLKARGEAATTPEHYNELGV
                     RKYDLPALARRLREHFRGPSTSNPKLVSYLAAGGIQGLRPLRYEKRVARNRFIFFVII
                     LFLALLGTLLMFNKGHR"
     gene            4189..4719
                     /locus_tag="VIL39_07305"
     CDS             4189..4719
                     /locus_tag="VIL39_07305"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5481270.1"
                     /translation="MQTTLKNDIAARIHGLPIPTPDGEFIARYSREGLCGLEFPIGTR
                     PRKTETGAARPPAQIRRWHAATSKALMLALAGRPPKSLPPLDLSGGTVFQQRVWRTLR
                     KIARGRISSYAQVARAIGNPKAVRAVGGACGANPIPVLVPCHRVLAANGGLGGFSGGL
                     NWKRALLKREGIKLPD"
     gene            complement(4771..6129)
                     /locus_tag="VIL39_07310"
     CDS             complement(4771..6129)
                     /locus_tag="VIL39_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481271.1"
                     /translation="MSGTALPTALVVHHPPGFRARRGVNWAFLGLLYTSFYMCRYNFS
                     IANKSISDEYGFSRGEMGYIITTSLFAYACGQIINGLLTDRIGGKRAMLIGAAGTIVM
                     NLLFGAASFAGMLGLFIAIRGVDGYLQAFGAPGMVKINTAWFAHRERGRFSGIFGFMI
                     NLGRLGIFTLGPALLAGFTILGLIHIPPLHWRWLFWIPSLICAGAAVGMALTVKETPE
                     EAGFPGVIADDTGSTDGGVTAQFSQVIRAILSNPAVWITACAYACTGAVRQAVDQWFP
                     RYMQDLYQVDLKSARFQFLAFLIPFVASAGSLIAGYVSDTLFKGRRAPVAMMLYFLET
                     AIILLAAQFHTANAAILFLVLISFTANSTHSILGSAAPMDIGGRRMAGFALGLIDSFQ
                     YFGGSLAGYFLGNLLDRSLGNYFYFMAPFGVIGGVLMLFIRGNITHGPKPGARPGNTA
                     QA"
     gene            complement(6194..>6460)
                     /locus_tag="VIL39_07315"
     CDS             complement(6194..>6460)
                     /locus_tag="VIL39_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481272.1"
                     /translation="AAADFGGRKATATCSGIVDGFTYLGTGVQAVSLGYLTGQSWLWW
                     PIFLMPFALLGSYLALKIWHELPVATRKYIAEVEEKAKIDRLPE"
BASE COUNT         1310 a         1936 c         2017 g         1197 t
ORIGIN      
        1 gccgctttgg tcgctctggc gttccgagaa caatcccaga gccggcgcca ccagccaatc
       61 gctgttgtgg aacctgtacc gacgcgaagc cacgcctgcc gccagaaagt gctcgctcct
      121 gttcgggctt ttccagtatc aatccagttc cgaaggcaaa gacatgcgtt tattctatat
      181 tcctctgggc ggggcgagac cccccgcgca gagcggcgcg aattccgcgc cggcaaagac
      241 ggcgcagtga agatgcgccg gacggggcga atcgcactcg ccgcggcaac cggtttgaag
      301 tatttagcaa tttagctgta gcagtatgtt tcaaaacatc ggagaaatgg tgctcctgtt
      361 ctggcgcact ctggtggcgc tgccgctggc gtggcgccag cggcagaagg tgttcgacca
      421 gttcttcgaa atcggcaatg ccagcctgct gatggtttgc atcctttcct tcttcattgg
      481 cggggtcatc gccctccaaa ccggcccgct gttggtcgag cgcggcttgg gcagttacgt
      541 gggtggaatc gtcggctgga cgattgccaa agagctggcg cccgtgatga tgggcatcct
      601 cattgctggc cgcatcggtt cggccatggc ggcggagatc ggctctatgc gcgtttatca
      661 ggagattgat gcgctccgca cgatgaacat cagcccgatc cactatttgg tgctgccacg
      721 cctggtcgcc atcgctgccg ccttaccctt gctggtaacc tttggcattc tcgtgggctg
      781 gctgggaggc tcggtggtgt gtgtggccaa cgaccggatc gccgtctcct tcgaggcgtt
      841 cttctcttcc ctccgcgacg ccgtggatct taaagacgtg gccaacggcg ttttcaagag
      901 cttcgtgttt gccctgacca ttggggtggt ctcctgccat caggggctca tcacccgagg
      961 cgggccgcgc ggcatcggcc gctcggttac caaagcggtc gtcaactcca tcgtcttgat
     1021 cgtgatcttg gattatctcc tgacgcggat cctgctgaac taatgaacgg ctcctccacc
     1081 aatcatcatg gcgtgggcat ccaggtgcgc ggcttgcgca agtcttttga cgggcaggag
     1141 gtcctgcggg gactggattt cgaggtgcag ccgggggaaa tcttcgtcat catgggcccc
     1201 agcggtagtg gcaagaccgt cttgctcaag catctcatcg ggctcgaaga gcccgatgcc
     1261 ggggaaatcc tgattgaagg ccagccgatt caaaccccgg aggtcatgag ccaataccgc
     1321 atggcgatgg tcttccagtc cggcgcactg ctcaattcgc tgaccgtcgg ggaaaacgtc
     1381 gggctttacc tcgccgaaca tcggctccag ccgccagcgg agatcgctcg cattgtcgct
     1441 gagaagctcg aagtcgtagg cctgaaaggc accgaagaaa agatgccgag cgagctttcg
     1501 ggcggcatga agaagcgcgt cgcgatcgcc cgcgctctgg tcatcgatcc gcaactcatt
     1561 ctctatgacg aaccgaccag cgagctcgac ccgctctcgg ccgtaatgat cgccgaggag
     1621 attttgcatc tcaaggaccg gatcggggtc acttcgctcg tcgtttcgca tgatcgggat
     1681 ctggcgttcg gcgtggccga ccggattgct gtcatcaacc agggtcggat tctgaccatc
     1741 ggcactcccg acgaggtgaa gcagtccccc gattcgctcg tgcagcaatt cctccacgcc
     1801 gatttcaagc gtggtcgcgc ccctttagaa cctagtaaag tgtatgaaaa acagtcttga
     1861 aacccgtatt ggattgttcg ttgccttggc cgtgctggcc gccgcgatcg ttttggagcg
     1921 agtgggcagc ttcgagatgt tcgagcgcgg caagcacgtg aaggcgctgt tcgccaatgt
     1981 gcaggacctc aaagtgggcg accgcgtgaa gatggccggc gtcgaaatcg gccgcgttca
     2041 ggggattcag atcaccaaca atcgagtgcg ggtcatgatg aaggtgcgcc ccaccgccga
     2101 ggttaagacc gacagcattg cgacgatcaa attcaccggg ttgctcggcc agaacttcgt
     2161 ctcaattgac ttcggcacgc cgcaggggga acctctgaag gaggatcaat atgtcgccac
     2221 tgccgagcag gccgacctga gcgccatgat gcagaagatt gacaacgtcg cttcgggcgt
     2281 ggagaacctt accaagagct tcaccggctt caagatcgat accctggtcg ggccgctcag
     2341 ggatttcatc agcgccaaca aagacccgtt gacgatgacc atttccaacc tccagaccct
     2401 ctcttccaac atctccgccc aggtcgagca gggccatggc acgttgggca aactcatcca
     2461 ggatgaaacg ctctacaaca ccgcactctc cacagtcagc aacctgcagg ataccgctat
     2521 tgagatcaag gcgacggtcg ccgacgcccg caagctcgtc gatcaggcca atgccggccg
     2581 aggcacggtc gggaagctgc tgacggacga ctcgcttttc cgggagacga ccaatgcgat
     2641 gaccaacctg aaggaaatcc ttcagaaggt taatcagggt cagggcacgg tcgggaaact
     2701 ggtcaacgat caggaattct acaagaacgc gaagctgacc ttgcagaagc tggaccaggc
     2761 gacggagggc ctggaagatc agggaccgct aagcgtcctc ggccttgccg taggaaagct
     2821 gttctgaacc ctgctttaca ccttgcgccg tgccgcttag attaaccgca tgtcttcgga
     2881 ttacgatgca acggagtttg tcgatggtga tttccaagcg cacaaggtgc ctgcctccgc
     2941 cacctcggcc ggtgggagtc aatcgcagcg cgcgccgacg cgcgaggagg tggactcccg
     3001 agtggctgag gcccagcaga agctcgcgga gctgaagcga gagcaggagg aactggaacg
     3061 tcaacgggcc gcgttggagg aaacccgccg gcgtcagatg gagtttcaga ctggccgcca
     3121 ggagacgctc cagaacctga cgcgcggggt gggtttgctc gaggaggccg agttcagtgc
     3181 gcggcgggac gccgagcaaa tggccaaagc gttggtgaat ttgcgcgatg cgctcgccaa
     3241 gctcgcggcc attcatgagg aggggtggac caaagacaat ttcagcatgg agctgacgcg
     3301 cgctttgacg gcgctggaga acgcccgaat ggagtggaat gccgcgcggc tgaaattccc
     3361 gattctgtcc aacccgtccc aggacggcag cgcgggcgcc gcggatgtcg ccgcgccacg
     3421 gacctccctc ctggggacac atagtttcgg cgagctttgc cggattggct tcgcgttgac
     3481 gtggccgctg gccgcagtgg cgctggtcgc tttggccgcg ctgctggtgt ttctagcgcg
     3541 tcactgaccc ggctgccgcc atggcatggt ttacaaaaaa gccggacccg atttccgacc
     3601 gcgcgcggac tctcaacgac gagatagcca agctcgaatc gagaatccag caactcgacg
     3661 cgcaagtgca gcgcggtcaa acccagccgc gtttgcggtc caccgctctg ccccacagca
     3721 gcacgatcag tcacgcgaca ccgacgtccg caccgccgcc ggagccggtt acccatgagc
     3781 cgatcttcga agaggtgggc caggaccggc taaaggcgcg gggcgaggcg gccaccacac
     3841 cggagcacta caacgagctg ggcgtgcgca agtacgacct gcccgcattg gcgcgtcgcc
     3901 ttcgggagca ctttcgcgga ccatccacgt ccaacccgaa gctggtgagc taccttgcgg
     3961 cgggcggcat ccaggggctt cgaccgctgc gttatgagaa gcgcgtggcg cgcaaccgct
     4021 ttatcttctt tgtgattatc ctgtttctgg ccttgctggg cacgctcctg atgttcaaca
     4081 aggggcaccg gtgagcagat ccgaaagccg aaggcttcag cgggttaacc ccgtctggcg
     4141 cacgtgaggt tttgatttcg aacgccagca gaccctattt gctcctgaat gcagacgacc
     4201 ctgaagaacg acatcgcggc acggatccac ggcctgccga tcccgactcc cgatggggaa
     4261 ttcatcgcgc gctattccag ggaaggtttg tgcggtttgg agtttccaat aggcaccagg
     4321 ccgcgaaaga ccgaaacggg cgcggcgcga ccgccagccc aaatccgccg gtggcatgcg
     4381 gccaccagca aggccctcat gctcgcgctc gcaggccggc caccgaaaag cttgcccccg
     4441 cttgatctat ccggcggcac ggtgtttcaa cagcgcgtat ggcggacctt gcgcaagatc
     4501 gcccgtggcc ggatttcgag ctacgcccaa gtcgcacggg cgatcgggaa tccgaaggct
     4561 gttcgtgccg tggggggcgc ttgcggcgcc aatccgattc ccgtgttggt gccttgccat
     4621 cgggttttgg ctgcgaacgg cgggctgggt ggtttttcgg gcggcctgaa ttggaagcgg
     4681 gcgttgctga agcgcgaggg catcaaactg ccggattgac gcggctgaat gggccagcgg
     4741 gattccccaa gtcctgccgg cctatttcat tcaagcctgg gcggtgtttc ccggccgtgc
     4801 tccgggcttc ggtccgtgag tgatattgcc tcgaatgaaa agcatcagca cgcctccaat
     4861 cacgccgaaa ggcgccatga aatagaagta gttcccaagg ctccggtcga gcaggttgcc
     4921 cagaaagtaa ccggccagac tgccaccgaa atactggaag gaatcaatca agcccaaggc
     4981 aaaccctgcc atgcggcgcc cgccaatgtc cattggcgcg gctgacccca ggatcgagtg
     5041 agtggaattg gcagtgaacg agatgagcac caggaacaag atagccgcat tggccgtgtg
     5101 gaactgggcg gctaagagga tgatggccgt ttccaggaag tagagcatca tcgccaccgg
     5161 cgcgcgtcgt cccttgaaga gcgtgtcgga cacgtagcct gcaatcaaag agcccgccga
     5221 agccacaaag gggatcagga acgcgagaaa ctggaatcga gccgatttca gatctacctg
     5281 ataaagatcc tgcatgtaac gcgggaacca ttggtccacg gcctggcgca ccgcgccagt
     5341 gcaggcatag gcgcaggccg tgatccacac cgcaggattg ctgaggatag cgcgaatcac
     5401 ctgcgagaat tgggccgtca cccccccgtc ggttgagccg gtgtcgtcgg cgatcactcc
     5461 cgggaatccg gcttcctccg gcgtttcctt gacggtgagt gccatgccga cggcagcccc
     5521 cgcgcagatc agcgagggga tccagaacag ccagcgccag tgcagaggcg gaatgtgaat
     5581 cagaccgagg atggtgaaac cggccaacag cgccggaccg agcgtgaaga tgcccaggcg
     5641 gcccaggttg atcatgaagc cgaagatgcc ggagaaacgc ccgcgctccc ggtgggcaaa
     5701 ccaggcggtg ttgatcttga ccatgccggg cgcgccaaaa gcctggaggt aaccgtccac
     5761 gccccgaatg gcaatgaaca gaccaagcat cccggcgaat gatgcggcgc cgaagagcag
     5821 gttcatcacg atggtgcccg cggcgccaat cagcatggcg cgcttgcctc ctatgcggtc
     5881 cgtgagcagc ccgttgataa tctggccgca ggcatacgcg aaaagcgacg tcgtgatgat
     5941 gtaacccatt tcgccccggc taaacccgta ctcatcgctg atggacttgt tcgcgatcga
     6001 gaagttgtag cggcacatgt agaacgaggt gtacaacagg ccgaggaagg cccagttgac
     6061 gccgcgccgc gcgcgaaagc cagggggatg gtggacgacc agggctgtcg gcaaggcggt
     6121 tccgctcatg gcgccttcag gccgcgccgt tcaggggagt gatggcaggc gacagtatgc
     6181 gccggtagag agatcattcc ggcagccggt caatcttcgc cttctcctct acttccgcga
     6241 tgtacttcct ggtggcgacc ggcagctcat gccagatctt caaggccagg tagctgccca
     6301 gcagggcaaa gggcatcagg aagatcggcc accagagcca gctctggccc gtcaggtagc
     6361 ccaggctcac ggcctggact ccggttccca aataagtgaa gccgtcaaca attccggagc
     6421 aggtggcagt ggcttttcgg cccccgaaat cggcggccgc
//
LOCUS       DATCGS010000358         6454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_28672, whole genome shotgun sequence.
ACCESSION   DATCGS010000358 DATCGS010000000
VERSION     DATCGS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28672"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3072
                     /locus_tag="VIL39_07320"
     CDS             <1..3072
                     /locus_tag="VIL39_07320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5481273.1"
                     /translation="LLERRFEEAIDHFLAVQQAEGASDAISSALAAAYHRLAFQTLAD
                     QVRRSVRSVRGNQWMFRMGHPSDHPLRLRPELLRPNPGDGTYPILRECTPVRMDLTHC
                     GWSDIFFLGMDFPEGAKVLNVSIDLGIHGRDAAARPPVEAYLRVIEEPLLRLTSVDLG
                     ATADITNLAEVFDFAKDYLGLLKAAIIAAGIVPPGIEGSGQSLAELLARIVGPGRGLE
                     IASSVNNIPKGSRLAVSTTLLAALVSVCMRATGQAASLTGQLQEHERRLVLARALLGE
                     WLGGSGGGWQDSGGVWPGMKLIEGVLASEGDPEYGISRGRLMPQHRILDQKDASAETR
                     RKLQDSLVLVHGGMAQNVGPILEMVTEKYLLRCDAEWKARQSMLGILDEILAALRGGD
                     VRAIGGATTRNFGQPIQTIIPWATNYFTETVIAQVRAEFGDAFWGFWMLGGMSGGGMG
                     FIFAPEHKTRAQQRLQEIMSATKRELQDALPFAMEPVVYDFAINEHGTFADLLEGGAA
                     LMPPGYYTLTAPALLRQDRHALSPLRRAELDKFGAACRNRPELRGVVQTLFDAMLPRG
                     KAESATDQTLAALLEQHGFDRVQHEHIRTDLKEGRIGLAQNRLPASAVIEDVQEGDVA
                     DASNLKSDSSGLAAVGLNALKNGEVAVVTLAAGAGSRWTQGAGVVKALHPFCKLAGRH
                     RTFMETHLAKSRRVSREAGTPIPHIFTTSYLTHAPTEEFLARQNHYGYQGPLFLSPGK
                     SVGLRMIPTVRDLRFVWEEMPQQILDEQQQKVRDSLRHALINWASSAGEASDYTDNLP
                     LQCLHPVGHWYEVPNLLRNGVLARLLAERPQLKYLLLHNIDTLGADVDPALLGGHIRS
                     GAGLTFEVITRRLEDRGGGLARVNGRVRLVEGLAMPREEAEFALSYYNSNTCWIDLDK
                     LLAAFALTRADLNNGEKVTAAIRTLAGKMPTYITLKEVKKRWGHGQEDIFPVTQFEKL
                     WVDMTALPEIDSRFVVVPRLRGQQLKDQAQLDGWLRDGSAAYVERLCEYQD"
     gene            3077..4315
                     /locus_tag="VIL39_07325"
     CDS             3077..4315
                     /locus_tag="VIL39_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481274.1"
                     /translation="MLRTVRKAEYAELVVLFFLQGAALSMWFVPLSTVLDANGLHAIK
                     PFAYAATALAAFVSPLIFGAMADRQASPVAVLRGLSLATAAAMVLASTAIKLGWNPWL
                     VLALIQLHALCSSPTFSIASTIVFARLADSQQEFGPIRAMATLGWMSGCWVVSALGAD
                     TSALAGYSGAVMWVLAAGFTYFLPELETPKSAEHLTWHERLGLDALTLLKNPDHRVVF
                     ITSALVCIPLAAFYPYAPPHLRQLGLQRTSAWMTLGQVTEVVAMFSLGALLVKWRVKW
                     VLACGLGFGVLRFALSAMNGRGWVLAGVLLHGCSYTLVSITAQIYLNQRIDTQWRARA
                     QALLALMNSGVGNLLGYLSTGWWFSHCTQLSGTQWPLFWGGLAAAVGLVLAYFLTAYH
                     GVGSGFMRVTENRQAAAVPD"
     gene            4456..4731
                     /locus_tag="VIL39_07330"
     CDS             4456..4731
                     /locus_tag="VIL39_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY5481275.1"
                     /translation="MSVDEDFVPKGDIGVQVVAGGDYAVTTHFGPYHKLGDTYTKLLG
                     QWLPRSGRELRSAPCFEVYLNDPQGTEPEDLLTDIYAPLQTITKSPK"
     gene            4728..5345
                     /locus_tag="VIL39_07335"
     CDS             4728..5345
                     /locus_tag="VIL39_07335"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY5481276.1"
                     /translation="MKPQPKIDLYKLHKAEYAATQKPALVELKSATYLAISGQGAPGG
                     ELFTSSIGALYGVAFTIKMTRKFAGQQDYAVCKLEGQWWGEPASNFAKLPQDQWLWKL
                     LIRTPDFIKKADLRQAVAVLLKRDKGEDVKRVGLESLAEGLCVQMLHVGPYDKECETI
                     GVMKEYAEKKQIEFSGKHHEIYLSDPRRVPPERLKTILRHPVRPA"
     gene            6072..>6454
                     /locus_tag="VIL39_07340"
     CDS             6072..>6454
                     /locus_tag="VIL39_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5481277.1"
                     /translation="MSSPKPTLDDLRIERSAEPESTSRFWPVAIGIVVLVLAAGGIWR
                     LKRPGAIAVHTVLVREASGNGIERTVLNASGYVTARRQATVSSKVTGKVTEVLVEEGM
                     KVQEGQVLARLDDTNVKTSLRLAQA"
BASE COUNT         1185 a         2049 c         2055 g         1165 t
ORIGIN      
        1 ctccttgagc gcagattcga ggaagccatt gatcatttcc tggcggtgca gcaggcggaa
       61 ggcgccagtg acgctatttc gagcgccctg gccgcggctt atcaccggct ggcatttcag
      121 acgctggccg accaggtgcg ccgaagcgtg cgctcggtgc gcggcaatca atggatgttc
      181 cgcatgggcc acccgtccga tcacccgctc cgcctccggc cggagctgct tcggccaaac
      241 ccgggcgacg gcacctaccc gattctgcgc gaatgcaccc cggtgcgcat ggatctcacg
      301 cattgcggct ggagcgacat cttcttcctg ggcatggatt tccccgaggg cgccaaggtg
      361 ctcaatgtgt ccatagacct tggtatccat ggacgtgatg ccgcggcgcg cccgcccgtc
      421 gaggcctacc tgcgagtcat cgaggaaccg ttgctccgcc tgaccagcgt tgatctgggc
      481 gcgacggcgg acatcaccaa cctggctgag gtgttcgact ttgcaaaaga ctacctcggc
      541 ctgctcaagg cggcgatcat cgcggccggc atcgtgccgc cgggcatcga gggatcaggc
      601 caaagcctgg ccgaactgct ggcgcgcatt gtcgggccag ggcgcggctt ggagattgcg
      661 agcagcgtca acaacatccc caaaggctcg cgtctggcgg tttcgacgac gctgctggcg
      721 gcgctggtca gcgtctgcat gcgcgcgacc ggacaggctg cgtcgctgac gggccagttg
      781 caggagcacg aacgccggct ggtcctggcg cgcgcgctgc tcggcgaatg gctcggcggt
      841 tccggcggcg gctggcagga ctcgggcggc gtctggccgg gcatgaaact catcgaaggc
      901 gtgctggcca gcgaaggcga cccggagtac ggcatcagcc gcggccggct tatgccgcaa
      961 caccgcatcc tcgaccaaaa ggacgcctcg gcggagacgc gccgcaagct gcaggatagc
     1021 ctagtgctgg tccacggcgg catggcgcaa aatgtggggc cgattctgga gatggtcacc
     1081 gagaaatacc tgctgcggtg cgacgcggaa tggaaagcgc gtcagagcat gttgggcata
     1141 ctcgacgaga ttcttgccgc cttgcgcggc ggcgacgtgc gcgcaatcgg gggcgcgacc
     1201 acccggaact tcggccagcc cattcaaacc attatcccct gggctaccaa ctacttcacc
     1261 gaaacggtga tcgcccaggt gcgcgcggag ttcggcgacg ccttctgggg cttctggatg
     1321 ctcggcggca tgtctggcgg cgggatgggc ttcatcttcg cgcccgagca caagacgcgc
     1381 gcgcaacagc gcctgcagga aatcatgtcc gccaccaaac gcgaactcca ggacgccctg
     1441 ccctttgcca tggagcccgt ggtgtatgat ttcgccatca acgagcacgg caccttcgcc
     1501 gacctgttgg aaggcggagc ggcgctcatg ccgcccggct attacaccct gaccgcgccg
     1561 gcgctactgc gacaggaccg ccacgcgctc tcgccattgc ggcgcgctga gctggataaa
     1621 ttcggcgctg cctgccgcaa ccggcccgag ctgcgcgggg tggtgcagac gctcttcgac
     1681 gccatgctgc cgcgcggcaa ggcggagtcg gcgaccgacc agacactcgc cgccctgctg
     1741 gagcagcatg gcttcgaccg cgtccagcat gagcacatcc gcaccgacct caaggaaggc
     1801 cgcattggcc tggcccaaaa ccgcctgcca gccagcgcgg tcatcgagga cgtgcaggaa
     1861 ggtgacgttg ccgacgcctc caacctcaaa tcagacagtt caggcctggc agcagtaggg
     1921 ctcaacgcat tgaagaacgg ggaggtagca gtcgttacct tggccgctgg cgccgggagc
     1981 cgctggacgc aaggcgcggg ggtggtaaag gcccttcatc ccttttgcaa attggccggg
     2041 cgccatcgca cgttcatgga gacgcacctg gccaagagcc ggcgcgtttc ccgcgaggcc
     2101 ggaactccca tcccgcatat cttcaccacc agctacctga cccacgcgcc gacggaggaa
     2161 ttccttgccc ggcaaaacca ttacggttac cagggtccgc tgttcctctc gcccggcaaa
     2221 tcggtcggat tgcggatgat ccccaccgtc cgcgacctgc ggttcgtctg ggaggaaatg
     2281 ccgcagcaga tcctcgatga gcagcagcag aaagtgcgcg acagcctgcg ccacgcgctg
     2341 atcaactggg ccagcagcgc gggcgaagcc agcgactaca cggacaatct gccgctccag
     2401 tgcctgcacc ctgtcggcca ctggtatgaa gtgcccaacc tcctgcgaaa tggcgtcctg
     2461 gcgcggctgc tggcggagcg tccgcagttg aagtatttgc tgctccacaa cattgatact
     2521 ctcggggccg atgttgatcc cgctctgctg ggcgggcaca tccggagcgg cgcgggcctc
     2581 accttcgagg tcatcacccg ccgcctcgaa gaccgtggcg gcggcctggc acgcgtcaat
     2641 ggccgggtcc gcctggtcga gggcctcgcc atgccgcgcg aggaagccga gttcgccctg
     2701 agctactaca actcgaacac ctgctggatt gatctcgata agctgctcgc tgccttcgcc
     2761 ctgacccgcg ctgatctcaa caacggggaa aaggtcactg ccgccattcg caccctcgca
     2821 gggaagatgc ccacgtacat cacgctcaag gaggtgaaga agcgctgggg ccacggccag
     2881 gaagacatct tcccggttac ccagttcgag aagctgtggg tggacatgac agcgctgccg
     2941 gagattgatt cccgcttcgt ggtcgttccc cgcctgcggg gccagcaact gaaagaccag
     3001 gcccagctcg atggctggct gcgcgacggc tcggcggctt atgtcgagag gctgtgcgag
     3061 taccaggact gaccgggtgc taagaaccgt tcgcaaggcc gagtatgccg agttggtggt
     3121 gctcttcttc ctccagggag cggcgctgag catgtggttt gtgccgttga gcacggtcct
     3181 cgacgccaac ggcctgcacg ccatcaaacc gtttgcttat gccgcgacgg cgttggccgc
     3241 ctttgtctcg ccgctgatct tcggtgccat ggccgaccgc caggcctcgc cggtggccgt
     3301 tttgcgcggc ctctcgctcg ccacagcagc cgccatggtg ctggccagca cggccatcaa
     3361 gctgggctgg aatccctggc tggtgctcgc gctgattcaa ctccatgcgc tttgttcgtc
     3421 accgaccttc agcatcgcct ccacgatcgt cttcgcccgt ctggcagata gccagcagga
     3481 gtttggccct atccgcgcga tggccacgct gggctggatg agcggctgct gggtagtcag
     3541 cgcgctcggc gccgacacct ccgccctggc tggctacagc ggcgctgtca tgtgggttct
     3601 ggcggcgggg ttcacgtact ttctgccgga gcttgaaacg cccaaatcgg cggagcatct
     3661 tacgtggcat gagcggctgg gcttggacgc gctcacgctc ttgaagaatc ccgaccaccg
     3721 cgtcgtgttc atcacttcgg ccctggtctg tattccgctc gcggcctttt atccgtatgc
     3781 tccaccgcac ctgcgccagc ttggcctgca acgcaccagc gcctggatga cgcttgggca
     3841 ggtgaccgaa gtcgtcgcca tgttcagcct tggcgccctg ctggtgaagt ggcgggtgaa
     3901 atgggtgctc gcctgcggcc tgggtttcgg cgtgctgcgg tttgcgttga gcgcaatgaa
     3961 cggcagaggg tgggtcctcg ccggcgtgct gctgcacgga tgttcctaca cgctggtctc
     4021 gatcacggcg cagatttacc ttaaccaacg catcgacacc cagtggcgag cgcgggcgca
     4081 ggctctgctg gctctgatga acagcggcgt cggcaacctg ctgggctacc tcagcaccgg
     4141 ctggtggttc agccactgca cccaactgtc cggaacgcaa tggccgctct tctggggcgg
     4201 attggccgca gcggtcggcc tggtgctggc ctacttcctc accgcctatc acggcgtggg
     4261 ttcgggtttc atgcgggtca cagaaaaccg tcaggcggcc gcggttcctg actgaagatg
     4321 ccaaggccca ttgcggggtc gcaaaagcgc aaaaacaatc aagcgctcca accgcacttt
     4381 tggtataagt gcgggcgaag attaggcacg ggacgatgcg aacggttaca ttgcaagact
     4441 acaaacgacg cctgcgtgag cgtggatgag gacttcgtgc ccaaggggga catcggagtg
     4501 caggtcgttg cgggcggcga ttatgctgtg acaacccact ttggccccta tcacaagctg
     4561 ggcgacacct acacgaagct gctcggtcaa tggctgcccc ggagcgggcg cgagctccgc
     4621 tccgcgccat gcttcgaggt ttacctgaat gacccgcaag gcaccgaacc cgaagacctg
     4681 cttacggata tttatgcacc gctgcaaacc ataaccaaaa gcccaaaatg aaaccgcaac
     4741 cgaagataga cctctacaaa ctccacaagg ccgaatacgc cgccacccag aagcccgcac
     4801 tggtagaact caaatccgcc acctatttgg ccatctccgg ccagggcgct cccggtggag
     4861 aactgttcac ctcgagcatc ggcgcactct atggcgtggc gttcacgatt aagatgaccc
     4921 ggaagttcgc cggccagcag gactatgcgg tctgtaagct cgaaggccaa tggtggggcg
     4981 agccggcctc gaacttcgcg aagctgcctc aagatcaatg gctctggaag ctgctgatcc
     5041 ggaccccgga tttcatcaag aaggcggatc tccgccaagc ggtcgccgtg ctgctgaaac
     5101 gggacaaggg ggaggacgtg aagcgggtgg ggctggaatc cctggccgag gggctctgcg
     5161 tgcagatgct gcatgtcggg ccttatgaca aagagtgcga aacgatcgga gtgatgaagg
     5221 agtatgcgga gaagaaacag atcgagttct ctggcaagca tcacgaaatc tacctctccg
     5281 atccacgtcg cgtcccaccg gaacggctca agacgatcct gagacacccg gtccggcccg
     5341 cttgaggcct gcgcgcccga gaacacggcg tatatcgagc ccttagcgtt agatgtgggc
     5401 gagcatacgg taatggcagg ccggcgcgca gagcatcacg ctgctcaccc gtgggcaagg
     5461 ctaattcccg gcatgcacct atatgtgttg gcgccgctgt ggcccgagtg cttggtttta
     5521 gaggttaacg ataacgctgc gtgagggcac gcggcctaca gggagtttgt aggccgcgtg
     5581 ccctcacgcg gcggcccgtg tatcgcttct tcgttcaaac aagctcaagt cccagttgga
     5641 catcccgggc gtcgtaatgc ggaccctccg ccagtgccac ggggacgcac gcatcccccg
     5701 gctcccttgg ggtgctaaac ggccccaaat tcgaaccccg tgcccgcagc agccgtcttt
     5761 gaggggcaag ggcagttgga cactaccggc aggttttcca ggttacaaat cgctctccgg
     5821 gacaggaagg gggcatccgc aggccaaagt ctgaagcgac ggcggcccgg ggggagtcgc
     5881 attacatcag atcaaggctt cctcgggttt cgggtttcgg ccttccttcg gccttcggtc
     5941 ttcggccttc ggatttcggc gctcaatatg ctcgtttgca cttcacggcc cgcacctcag
     6001 cgccgcgccg tccggcactc cataattgac cctgaggcga aaaagcggtt ccctgtgcgc
     6061 ggtctttatc tatgagttcg ccaaaaccta cgctggatga tctgcgcatc gagcgaagcg
     6121 ctgaaccgga gtcaacgtcc cgattctggc cggtggcgat tggcatcgtc gttttggtgc
     6181 tggcggcggg ggggatctgg agattgaagc ggccgggagc catcgcagtg cacacggtgc
     6241 tggtgcgcga ggcatccggc aacggcatcg agcgcacggt cttgaatgcc tcgggctacg
     6301 tcacggcccg gcggcaggcc acggtttcgt ccaaggttac cggcaaagtc accgaagtgc
     6361 tcgtggagga agggatgaaa gtgcaggaag ggcaagtcct cgcgcgcctc gacgatacga
     6421 atgtgaagac cagcttgcgc ctggcacaag ccca
//
LOCUS       DATCGS010000359         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_674803, whole genome shotgun sequence.
ACCESSION   DATCGS010000359 DATCGS010000000
VERSION     DATCGS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674803"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIL39_07345"
     CDS             complement(<1..324)
                     /locus_tag="VIL39_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481278.1"
                     /translation="MSADRREFLATAGAAFTSSIFTGNLKGANDRIAVGFIGAGRQGN
                     TNLGNAMRQPGVQVAAICDVYQPRVESAVAAAQRAGHQPKGCKDFRDVLADKSIDAVC
                     IATPDH"
     gene            824..976
                     /locus_tag="VIL39_07350"
     CDS             824..976
                     /locus_tag="VIL39_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481279.1"
                     /translation="MAAPGASFTVNNGAAAVGQSEITELAQFVHDHVRSWLQLELRNQ
                     AAAIVE"
     gene            complement(1347..2123)
                     /locus_tag="VIL39_07355"
     CDS             complement(1347..2123)
                     /locus_tag="VIL39_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481280.1"
                     /translation="MHRNAIHSYPKRFGHLLAATALLVASAFLAIAQANVPVTDLKVG
                     DWAEVLFGEWTPVTIAAPLSLGAYQVNVGAMVVSVAAYSSKIRHYTPTAADLAVQHET
                     AIAMQNRPKGPIGAKFGTREPATCPNRKNPPSAVTAKQYFICDWEATVLPLQISLVSD
                     VTIELGAARPFNYNQDSASTGIDVRSVVYDARGKYTSYQCIPQSAALNDFANAHNCNK
                     YPYTTAQGRCYKDTFGDWHCWMGGGAGGGQVTNQMPPAGF"
     gene            complement(2428..2501)
                     /locus_tag="VIL39_07360"
     tRNA            complement(2428..2501)
                     /locus_tag="VIL39_07360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2467..2469),aa:Gln,seq:ttg)
     gene            complement(3233..>4156)
                     /locus_tag="VIL39_07365"
     CDS             complement(3233..>4156)
                     /locus_tag="VIL39_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481281.1"
                     /translation="PDPALTASYAKSNGLAPELLFTDLAAMLDRAQPEAVATFTSTFD
                     HAMVVEACARRHIPVMMEKPLAVDMTQARAIQKAARSSGIPVMVNYETTWYKSHGEIW
                     KIVKERKGVGEIRKMVAMDGHQGPKEIKVQPEFLAWLTDPVKNGAGALFDFGCYGANL
                     MTWLMDGQRPLAVTAIAQTDKPAIYPRVDDEATVLVQYPKAQGIIQASWNWPAGRKDL
                     EVYGETGYAIATGGDTLRVRLPGQDAEVARQPADLPPAERDSITYFAGVARGQFKPSG
                     LNSLENNVIVVEILEAARESVRTGRKISLAN"
BASE COUNT          748 a         1227 c         1335 g          846 t
ORIGIN      
        1 gtggtccggc gtggcgatgc ataccgcgtc tatggactta tccgcgagga catcccgaaa
       61 gtccttgcac ccttttggct gatggcccgc tctctgggcg gcggccaccg ccgactcgac
      121 cctcggctgg tagacgtcgc agatggccgc gacctggacg ccgggttgcc tcatggcgtt
      181 gcccagattc gtattcccct ggcgtcccgc accgataaac ccaacagcga tgcgatcatt
      241 cgcgcccttc aggttccccg tgaaaatgga ggatgtgaac gctgctcccg cggtcgccag
      301 gaactcccta cgatcggcac tcatgtactt acaccctttc tctcacggac aaaaacagcg
      361 ccggcaggac cggccgcgct acaccgcttc gatggctggc ttgtcatacg ctggcgtcag
      421 ctatcagtca ggatttctat tttggcatcc tgaacaccgc tactttaaca ccgaaccgtt
      481 tccgttgggg tcggttttcc atccttccca tgcccccgat tttttgggaa cgttcgggat
      541 aacggtcctg ccggaagcgg ctgctgtcaa caaaaacgag agccagagtt cgcaaccaaa
      601 cggtttggct ccccagtctg gacacgtttc gaactttcgc tacccagtta gccctgtgat
      661 cgaacattgg acgcgcattg ttctcagcgt gatccgcccg gtcgccgatg tctgtccacg
      721 gcgggtgccc agtctggaca cgtctcgaac tcgctaagag cgcccggata gcttccggat
      781 gcgtcatcca tgccctattc caacttgcca ggatcccgcg agtgtggccg caccgggtgc
      841 ctcgttcacc gtcaacaatg gggctgcggc ggtggggcag agtgaaatca cggagctcgc
      901 ccaattcgtt catgaccacg ttcgcagttg gctccagctt gagttgcgca atcaggcggc
      961 ggccatcgtc gagtagcgtc ggcgtcacgg atgtctgcgc gagaatagcc gtggccgctg
     1021 gcacacctaa ggcaaagacg accaggaacc gtccgaccag catcggcttg ctgagccctc
     1081 gtgctggatt accccgttgc atttctcagc gttctggaag caatagatgt tgcttcctgg
     1141 gcatcccatg gcacgtaaag gcccacgatt tgaggtgggc acagaacggt tggagtaaag
     1201 ctcgctgaga cacgaggtgc aagaactgta gcttgaagtg ttaattgtgc cgtccgggat
     1261 ctgtagcgaa atgccccgct tctctttcgc ggcggcgcgt cttcgttggc ggctcgagcg
     1321 ggagatgagc tttaggcata gattcgctag aacccggcgg gaggcatctg gttcgtgact
     1381 tggcctccgc cggcgccgcc gcccatccag cagtgccagt cgccgaaggt gtccttgtag
     1441 cagcggccct gggcggtggt gtaggggtac ttattgcagt tgtgcgcgtt ggcaaagtcg
     1501 ttcaatgcgg cactctgggg gatgcactgg tagctggtgt acttgccgcg ggcgtcgtag
     1561 accacggacc tgacatcaat gccggtggag gccgagtcct ggttgtagtt gaaggggcgc
     1621 gcggcgccga gttcgatggt gacgtcgctg acgaggctga tctgcagcgg gaggacagtg
     1681 gcctcccagt cgcagatgaa gtactgtttt gcggtgacgg cgctgggtgg gttcttgcga
     1741 ttcgggcagg tggcgggctc gcgagtgcca aacttcgcgc cgattgggcc cttggggcgg
     1801 ttctgcatgg cgatcgcagt ttcatgctga accgcgagat cggcggcggt gggcgtgtag
     1861 tggcggatct tgctggagta tgcagcaacg cttacgacca tggcgccaac attgacctgg
     1921 taggcgccga gtgagagcgg agcggcgatg gtaacgggag tccactcgcc gaacaggacc
     1981 tcggcccagt cgcccacttt caaatcggtc acgggaacgt tcgcctgcgc aatggcgagg
     2041 aaggcggagg ccaccagcag agctgtggct gcgaggagat ggccgaaccg tttgggatac
     2101 gagtggatgg cgttgcgatg catgctgatt ctgcctcatg gcagggctaa accgcaattg
     2161 ccattttgga cttccaagtt gtgtttgtca actaggtgat gcgggcaatg cggaagtggc
     2221 gtcttgccga ttctgtccgg tatgtcggaa gttggcagaa gtggcggacc cgtgccgatt
     2281 tgtcagggga tgctggtcgg acgaatttcg ttgtcaagag ttcgagtcca cgccggggtc
     2341 accgtcaagt ggggccaatc cagaaggaac gcctgaaatg cccgtaatga aatcaatcac
     2401 atagcgtgaa gtcgagaagt ttcagactgg ctccccggcg aggattcgaa ccacgattca
     2461 cggctccaaa ggccgctgtc ctaccattag acgaccgggg aatgaaacgt agaggctgct
     2521 ctcgcttagt gtagcgtcat cagcgggttg cgcgcaacgc tcgctcgcgc gcggctgatc
     2581 ggcaggaact tcgcacaagt gggactggtt gggaaccgcc gggaaaccgg gccgactgcc
     2641 aatcggccgc ccgcggcggg cgcaacagga aggctgcgcg gcttggaagg cgacctcccc
     2701 cgagatttac cttctggccg cgcagccacc taactaaagc ggtctctctc ttgtagcgcg
     2761 cccctcccgc aaaagccaga tgcatttacg tcccggccac ccttttcaac aggttctttt
     2821 cgctaggcat ccttgcactt atccaggcag gggtgtggaa aagttgggaa taaaacggcc
     2881 ggcggtcgaa ctgtttcagg gccgagccgg tgcgcgacgt ccctcagcct ggtaccccga
     2941 ccgtctgatt ttgttccccg gtgctgcggg ccgggcatgc ctggccccta cagtgcccca
     3001 gtaggggcga ggcattgcct cgcccgcgcg ccacatgctg ccaacggctg gggaagaaat
     3061 cgcacggtca agaaaagcca agattcgacg acgggaagtt gccgatgttc ggaaagatgc
     3121 tggcgaggtt cgccgggccg acgccggacc actgggccac ggtcgtggcg cacgcactcg
     3181 tggagtggcc tttcaggctg gggggcgccc ggagcaaagt ggacagtatt gcttagttcg
     3241 ccaatgagat cttacggccg gtgcgcacgc tttcgcgcgc ggcctccagg atttccacca
     3301 cgatgacgtt attttccagc gaattcaggc cggagggttt gaattggccg cgcgccacgc
     3361 cggcgaagta agtgatggaa tcgcgctcgg cgggcggcag gtcggcgggc tgacgcgcca
     3421 cttcggcgtc ctggcccggc agtcgcacgc gcagggtgtc gccgccggtg gcgatggcgt
     3481 agccggtttc gccgtagact tccaaatcct tgcggcctgc cggccagttc caggaggcct
     3541 ggatgatgcc ctgcgccttg ggatactgca ccaagaccgt ggcttcgtcg tcgacgcggg
     3601 ggtagatcgc cggcttgtcg gtctgggcga tggccgtgac agccaggggc cgctggccgt
     3661 ccatcagcca agtcatcaga ttggcgccgt agcagccgaa atcgaaaagc gcgcccgcgc
     3721 cgttcttcac cgggtcggtg agccaggcga gaaactccgg ctgaaccttg atctccttgg
     3781 gcccctggtg cccgtccatg gccaccattt tgcgaatctc ccccaccccc ttgcgctcct
     3841 tgacgatttt ccatatctcg ccgtggctct tgtaccacgt ggtctcgtag ttcaccatca
     3901 cgggaatacc cgaactccgc gccgccttct ggatcgcgcg cgcctgggtc atgtcgacgg
     3961 ccagcggctt ctccatcatc acggggatat gccgccgggc gcaggcttcg accaccatgg
     4021 cgtgatcgaa ggtgctcgtg aaggtcgcaa ccgcttcagg ttgggcgcgg tccagcatgg
     4081 cggccagatc ggtgaacaga agttcgggcg cgaggccgtt gctcttggca taagacgccg
     4141 tcaaggcagg gtcggg
//
LOCUS       DATCGS010000360         7131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_130673, whole genome shotgun sequence.
ACCESSION   DATCGS010000360 DATCGS010000000
VERSION     DATCGS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130673"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1161
                     /locus_tag="VIL39_07370"
     CDS             262..1161
                     /locus_tag="VIL39_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5481282.1"
                     /translation="MKTLDIVLSGKLLLAGIILGCAFGFTAPAQAAPKKVLVVTVTKG
                     FRHSSIPTAEKVLAELAKESSAFTVEYARVNPDDPQFKGADGKRDNAKVDAAIKEVLA
                     EKMSAAALKQYDAVIFANTTGDLPLPDNQAFLDWIKSGKGFVGMHSATDTFPGFPGYT
                     EMIGGHFKAHGAQVKVDAINQDQECPICKHLPANWEVFDEIYQMKDFDRTKVHGLLTL
                     DKHPNDKTPGDYPIAWCKEYGNGRVFYTSLGHREDVWDPKWPDRKNSPEVAEAYQKHI
                     LGGIKWALGLEKMNAKPQKTAVE"
     gene            1223..2596
                     /locus_tag="VIL39_07375"
     CDS             1223..2596
                     /locus_tag="VIL39_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481283.1"
                     /translation="MNENESEALGYNRRDFLRGGSVATLMTMLGGVQLFAQTNAAPAG
                     DAKAAAAKIKVAVIGLGVWGREIINTLARVPQADVTAICDTYPASLKRTASAAPGAAQ
                     TADYKTILDDKDIKAVVVATPTFQHKEVVLAALKAGKHVYCEAPLAGSIEDAKEIALA
                     AKAAKNLVFQAGLQMRSEPQSNFLLPFIRSGALGKTVLARAQWHKKTSWRTASPNPER
                     EKALNWRLSKATSLGLIGEIGCHQIDHTGWFLNGLPKAVSGWGSVMFWKDDRDVPDTI
                     QAVLEFPGGVQMIYDATLANSFEGEYEVLYGSDAAVMMRESKAWMFKEVDSPLLGWEV
                     YAPKTTFYKETGITLAAGNSKPPPTDAQGQPIQPDPLTTTPLYSALGNFLRNAVDLSA
                     AAEDYKSSYGADDVDGLMEHLGKVPRRAAAGYLEGFQAAVTAVKANEAILSAKRIELK
                     PEWYELS"
     gene            2652..3329
                     /locus_tag="VIL39_07380"
     CDS             2652..3329
                     /locus_tag="VIL39_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5481284.1"
                     /translation="MKKRTIIAKTVALLLLAMVSLPAAAQTTTLAARSGSKMRIEGTS
                     NIHDWQCESPFIGGMIEVGPNFPLEPGQAVTPGKVEAKGDVFIQVRSLKSIEKDGKPY
                     MDSMDNVMWEHMRETENKRILYHLTELTLKEAPKAKDAPYVFDAQGELAVAGVTNKLS
                     MVVNVLPLGEKKLKITGTTNLKMTSFKIQPPAPKIAMGMIKTGDDIKLIFEWMLAPRK
                     TPGAAAK"
     gene            complement(3340..4005)
                     /locus_tag="VIL39_07385"
     CDS             complement(3340..4005)
                     /locus_tag="VIL39_07385"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEY5481285.1"
                     /translation="MNYTVRRASAAPQLHVEWSDPVWAQADTLEIQHFRPESSGHHPQ
                     TAARLLYDQHGIYGIFRIQDRYVRCLWTQYHDPVWKDSCVEFFAQPKPERGYFNFEFN
                     CGGAFLCCYITNPERTPEGFKEFIKVPAGIGETIRTRSSLPARIETEIVEPVVWTLSF
                     FIPFALFKHYLGPLGTSAGQEWRGNFFKCADETSHPHWASWSPVDELNFHRPDCFGAI
                     RFA"
     gene            4307..4915
                     /locus_tag="VIL39_07390"
     CDS             4307..4915
                     /locus_tag="VIL39_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481286.1"
                     /translation="MATNADTPLDQLWKEYGQGFRDFDDLTLARWLAQTLGQLKGRAW
                     RHSHPLLGAYRLAAELAHERQIWLMRLVAIPPAYSESPCCRAPMLPLLTRDVREAGLI
                     CQHCSETLVPFDEIPAGLQGELGDWAATYAPVHAVAHWDDRQRKDAGDYDHAYENAAD
                     EAEQLLAQAGRQFAPRLLEFYAATVWEDQDECLEVRPEDVPL"
     gene            complement(5166..6689)
                     /gene="dacB"
                     /locus_tag="VIL39_07395"
     CDS             complement(5166..6689)
                     /gene="dacB"
                     /locus_tag="VIL39_07395"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY5481287.1"
                     /translation="MKTTPKILCLLFAALLASSALAQEQKPPTTLVELRQRLATHIGQ
                     PKYDAALWGVKIISLDTGITVFEHNPQKLFSPASNSKLYTVALALDRLGADYRIKTSL
                     YAKAKPSRAGTLKGDLIVYGRGDPTLNARLHGGDIYKALEPLVFALTNAGVKSIAGDL
                     VGDESYFHGPPFGSGWTWDDLEYYYGAEISALTINDNTLQATVKPGARIGAPCQLTLQ
                     PATTWLSLTNRTATGQKGATRKLHFDHPLCQNVICVTGQMPLDDPGYTNEVTLHNPAG
                     LFVSFFKQALARQGIRVTGKLRSVNWLNPEARPLDDDKLVELGSVESLPLRDIAREVL
                     KPSQNLYTDLLLAHVGEKFRPANSPAEETSEGLGIRELNKFLAEAGLKRGEVIFEEGS
                     GLSRNNLTTPNATVALLQFMHRHKCAQAYLEALPIAGVDGTLRSRMKGMPAAGNVRAK
                     TGTLRWANSLSGHVTTAAGEHLLFSLMVNRYYEARPARGDLDTIGALLASFTGRTKE"
BASE COUNT         1452 a         2047 c         2317 g         1315 t
ORIGIN      
        1 gccatgaaga cgccttttca acaccacagc ggcctgcaag ccaccccaaa gccaccctca
       61 tacgactctc aagccagggg aatgggagtt gtcgaagcgg tggtgtcttt gaggtcgcga
      121 ggacatgccg agcgtggccg agaactgtgg ccggtcggtt actgcgacag aaaccttgac
      181 cgggaggcga aaaatgaatt tcattgtgag gaaacgagtc tcacgactta ctgatacgtg
      241 tgccgtagtc caattaaata tatgaaaaca ttagacatcg tcctgtcggg caagttgctt
      301 cttgctggaa ttatccttgg gtgtgccttt gggtttaccg cgccggcgca ggctgcgccc
      361 aagaaagtgt tggtggtaac ggtgaccaag gggtttcggc acagctcgat tcccaccgcc
      421 gagaaggtgc tggccgaact ggccaaagag agcagtgcgt tcacggtgga gtatgcgcgc
      481 gtaaaccccg atgacccgca gttcaagggc gccgatggca agcgggacaa tgccaaggtg
      541 gatgcggcga tcaaggaggt gctggccgag aaaatgagcg cagcggcgct caagcaatac
      601 gacgcggtga tctttgccaa taccacgggc gatttgccgc tgccggataa ccaggcgttc
      661 ctggattgga tcaaatcggg caagggcttt gtggggatgc actccgccac cgacaccttc
      721 cccgggtttc cgggatatac cgaaatgatt ggcggccatt tcaaggccca cggcgcgcag
      781 gtaaaggtgg acgcgatcaa ccaggaccag gaatgcccga tctgcaagca tctgcccgcc
      841 aattgggagg tgttcgatga gatttaccag atgaaggact ttgatcggac caaggtgcat
      901 ggcctcctga cgctggacaa gcatcccaac gacaagacgc cgggggacta cccgattgcg
      961 tggtgcaagg agtatgggaa tgggcgcgtg ttttacacct cgctcggcca tcgggaagat
     1021 gtgtgggacc cgaagtggcc ggaccggaag aactcgccgg aagtggccga ggcttaccag
     1081 aagcatattc tgggcgggat taagtgggcg ctgggattgg agaagatgaa tgccaagccg
     1141 cagaagacgg cggtggaata gtccatagtc caaagtccaa agtccaaagt ccaaagtccg
     1201 aaggacaaga gcgttgattt atatgaacga gaatgagagc gaggcgctgg gatataaccg
     1261 gcgggatttc ctaagaggcg gctcggtcgc gacgctgatg accatgctgg gtggagtgca
     1321 gcttttcgcc cagaccaatg cagcgcccgc cggcgacgcc aaggctgctg cggcgaagat
     1381 caaagtcgca gtcattggct tgggcgtgtg gggccgggag atcatcaaca ctctggcacg
     1441 ggtgccgcag gcggatgtta ccgccatttg cgacacctac ccggcgtcct tgaaacgcac
     1501 tgccagcgcc gcgcccggcg ctgcgcagac agcggactac aaaacgattc tcgacgataa
     1561 ggacatcaag gcggtcgtgg tggccacgcc gaccttccag cacaaagagg tggtgttggc
     1621 ggccctgaaa gccggcaagc acgtctattg cgaagcgccg ctggccggca gcatcgaaga
     1681 cgcgaaagag attgcgctgg ccgccaaggc ggcgaagaac ctggtctttc aggctggcct
     1741 gcagatgcgg tcagaaccgc agtccaactt tctgttgccg ttcatccgct cgggggcgtt
     1801 gggcaagacg gtcctggcac gagcacagtg gcacaagaag acaagctggc gaaccgcctc
     1861 gcccaacccg gagcgggaga aggcgctcaa ctggcgtctg agcaaggcga cttcactcgg
     1921 cctgatcggc gagatcggct gtcaccagat cgaccatacg ggctggttcc tgaatggcct
     1981 gccaaaggcc gtctccggct gggggtcagt catgttttgg aaggatgacc gcgacgtgcc
     2041 ggacaccatc caggcagtcc tggagttccc cggcggcgtg cagatgattt acgatgccac
     2101 gctggcgaac tcgtttgagg gcgaatacga ggtgctgtat ggcagcgacg cggcggtcat
     2161 gatgcgcgag agcaaggctt ggatgttcaa ggaagtggat tccccgctgc taggctggga
     2221 agtctatgcc cccaagacca cgttttacaa agagacgggc atcacgctgg cggccggcaa
     2281 tagcaagccg ccgcccacgg atgcccaggg gcagccgatt cagcctgacc cgctgaccac
     2341 caccccgttg tattccgctt taggcaactt cctccgcaac gccgtggacc tcagcgccgc
     2401 ggcagaagat tacaagtcca gctacggcgc ggacgatgtg gatgggctga tggagcacct
     2461 cggcaaagtg cctcgacggg cagcggctgg ctacctggag ggtttccagg cagcggtcac
     2521 cgctgtcaaa gccaacgagg caatcctcag cgccaaacgg atcgagctga agccggagtg
     2581 gtatgaattg agttgatggg cacgaagtcc aaaaactgca taacctgatg ggtcaaacca
     2641 ataatacaaa catgaagaaa cgaacaataa tcgcgaaaac agtcgccctt ttgttgctgg
     2701 caatggtgtc gttacccgcc gccgcccaga cgacgacctt ggccgcaagg tcgggcagta
     2761 agatgagaat cgaaggcact tccaacatcc atgattggca gtgtgaaagc ccgttcatcg
     2821 ggggcatgat cgaggtaggt cccaatttcc cgttggaacc cggccaggcc gtgacgcccg
     2881 gcaaagtgga ggccaagggg gacgtgttca tccaggtgcg ctcgctcaag agtatcgaga
     2941 aagacggcaa gccttacatg gactcaatgg acaatgtcat gtgggagcac atgcgggaga
     3001 ccgagaacaa gcgaatcctg tatcacctga ccgagcttac gctgaaggag gcgcccaaag
     3061 ccaaggatgc accctatgtg ttcgacgcgc agggtgaact cgcggtcgcc ggcgtgacca
     3121 acaagctctc gatggttgtc aatgtgctcc cgctgggcga aaagaagctc aagattacgg
     3181 gcaccacgaa tctcaagatg acgagtttca agatccaacc gccggctccc aagattgcga
     3241 tgggcatgat caagacgggc gacgacatca agctcatctt cgagtggatg ctcgcgccgc
     3301 ggaaaacccc tggcgcagca gccaagtagc cggcttcgcc taagcgaacc gaatcgcccc
     3361 gaagcaatct ggccggtgga agttcaattc gtccaccggc gaccacgagg cccagtgcgg
     3421 atgcgaggtc tcatccgcgc acttaaagaa gttgccgcgc cactcttgcc ccgcgctcgt
     3481 gccgagcgga ccgaggtaat gcttgaagag ggcgaagggg atgaagaagc tgagcgtcca
     3541 gaccactggt tccacaatct cagtctcgat gcgggccggc agcgaggagc gggtccggat
     3601 cgtttctcca ataccggccg gcaccttgat gaactccttg aatccctccg gcgtccgctc
     3661 ggggttggtg atgtagcagc agaggaaggc gccgccgcag ttgaattcga agttgaagta
     3721 gccgcgctct ggcttcggct gcgcaaaaaa ctccacgcaa ctgtccttcc agacggggtc
     3781 gtggtattgc gtccacaggc agcgcacgta acggtcctgg atgcggaaga tgccgtaaat
     3841 gccgtgctgg tcatagagca gccgggcggc cgtttggggg tggtgtccgc tgctttccgg
     3901 ccggaaatgc tgaatctcca gggtgtctgc ctgggcccaa acggggtccg accactccac
     3961 gtgaagctgc ggcgcggcgc tggcccttcg aacggtgtag ttcatgcccg cctattcaaa
     4021 gccgcgttca ttggcagcgc aacaaggaaa acgccataag gggaacggcc tcgaacccgc
     4081 cgggggcgct ctcgaattat ggtaattacg ctcggaattc cccccaatcc ggcccatgcg
     4141 gccccatccg ttaaccacgc caagtcggac aaagcctccc ccaaatccac gctaaagcca
     4201 tctacttgaa gggcgccctt cgtatcccgc ggctcaccgg caactcctca aagatgtgcg
     4261 atcctggctc gacaattttt accgggcgcg caacactgtc ggaccaatgg ccaccaacgc
     4321 cgatactccg ctcgaccagc tctggaaaga gtatggccag gggtttcgcg atttcgatga
     4381 cctgacgctg gcacgctggc tggctcaaac actcgggcag cttaagggca gggcctggcg
     4441 gcattcacat ccgctgctcg gtgcctaccg tttggccgcg gagttggccc acgagcgcca
     4501 gatctggctc atgcgcctgg tggcgatacc gcccgcttac tccgaatcgc cttgttgccg
     4561 cgcgccgatg ctgcccctgc tcacgcggga tgtgcgcgag gccggcttga tctgccagca
     4621 ttgcagcgag acgctcgtgc cgttcgacga gatccccgcg gggctgcaag gcgaactggg
     4681 ggattgggcg gcgacatacg cgccggtcca cgccgtcgcc cactgggacg atcggcagcg
     4741 caaagacgcg ggtgactacg accatgctta cgaaaacgcc gccgatgaag ccgagcagtt
     4801 gctggcgcag gccggccgcc agttcgcgcc ccggctgctc gaattctatg cggcgacggt
     4861 ctgggaagac caggatgagt gtctcgaagt gcggcccgag gatgtgccac tgtgaaggcc
     4921 gaaacccgag ggccgaagga aggccgaagt ccgaaatcag aagaggctga gggcccgtgc
     4981 tggagagggg gcctatcgtg cgctcaggcc agacggttgc tgtgctgggg gtaagcgtgc
     5041 tccggggggc acatcgggct gggtggtttc gggtttcggt gttcgggttt ccttcggtcc
     5101 tcggccttcg ggtttcggat ttgtgtcgcc gcgtgctcgc ttggcggaaa cgttgacggg
     5161 ccagttcatt ctttagtgcg ccccgtgaag ctggcgagca gcgcgccaat tgtgtccagg
     5221 tcgccgcgcg cggggcgggc ttcgtagtag cggttgacca tcaacgagaa gagcaggtgc
     5281 tcgcctgccg ccgtggtcac atggcctgac aggctattag cccagcgcag ggtgccggtt
     5341 ttggcgcgca cgttgccggc ggctggcatg cctttcatcc ggcttcgtag cgtgccgtca
     5401 accccggcaa tgggcagcgc ctcaagatag gcctgggcgc acttgtgccg gtgcatgaac
     5461 tggagcaagg ccaccgtggc gttgggcgtc gtcaggttgt tgcgggaaag gccggagccc
     5521 tcctcgaata tcacctcgcc gcgcttgagg ccggcttcgg ccaggaactt gttcagctcg
     5581 cgaattccga ggccttcgga agtctcctcg gcgggcgaat tggctggccg gaacttctcg
     5641 ccgacgtgcg ccagaagcag gtcggtatag agattctgcg agggcttcag cacttcacgc
     5701 gcgatgtccc gcagcggcag cgattccacc gagcccagct cgaccagctt gtcgtcgtcg
     5761 agcggccgcg cctccgggtt cagccaattg acgctccgca gcttgccggt gaccctgatt
     5821 ccctggcgcg ccagggcctg tttgaagaac gagacgaaga ggcccgctgg attgtgcaga
     5881 gtgacctcat tggtatagcc cgggtcgtca aggggcatct ggccagtgac gcaaatcacg
     5941 ttctggcaga gcgggtggtc gaaatgaagc ttgcgcgtcg cgcccttttg gcccgtcgcc
     6001 gtccggttgg tgagcgagag ccaggtcgtc gcgggctgca gggtcagttg gcacggggcg
     6061 ccgatacggg ctccgggctt gacggtggct tgtagcgtgt tgtcattgat ggtgagggct
     6121 gaaatctccg cgccatagta gtattccagg tcatcccacg tccaacccga tccgaacggc
     6181 ggaccgtgga agtagctttc atcgcccacc aggtcgccgg cgatggattt cacccccgca
     6241 ttggtcaggg caaataccag gggttcaagc gccttgtaaa tatcccctcc gtgcaggcgc
     6301 gcattgaggg tcgggtcgcc ccggccatag acaatcaagt cccctttgag cgtgcccgcc
     6361 cggctgggtt tggctttggc atagagcgag gtcttgatcc ggtaatcggc gcccagccgg
     6421 tcgagcgcca gggcgacggt gtacagcttg gagttggagg ctgggctgaa cagcttctgg
     6481 gggttgtgct cgaaaacggt gatgccggtg tccagggata tgattttcac gccccagagg
     6541 gcggcatcgt acttgggctg cccgatgtgg gtggccagtc gctgtcgcag ctcgaccagc
     6601 gtcgtcggag gcttttgttc ctgggccagg gcgctggacg caagcagagc ggcaaacagg
     6661 agacagagga tcttgggagt ggttttcact ggacgagaaa gttgtgggcg cgaaaagcgt
     6721 ttcactgtcc gaatactgcc gtccttgccg cgcagggcaa gctgaaacca gggcggttga
     6781 cagagtagcg taggcatccc cgcctgcgag tttcggcggc gtcccgccgc cagaaccagc
     6841 gccaattcat gaaggccctt tggcgcaaag gaggtgcttc actctgggct gctggctacg
     6901 gggtgattcg tgctcagggg ctagcgcccc atgggctgag ggttctggaa gcggcaagag
     6961 ccccgcaggg tggagttcca agcgcgtcag gatggggcgg ccccctggat gccctccagg
     7021 cgctcgacac cacccccggc ccggtgtcgc tagggtgtct ccaccgtatt gctacggtat
     7081 tgctacggta tctccccggt gttgctccca tgttgatgcc atcaacatgg g
//
LOCUS       DATCGS010000361         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_625185, whole genome shotgun sequence.
ACCESSION   DATCGS010000361 DATCGS010000000
VERSION     DATCGS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625185"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1237
                     /locus_tag="VIL39_07400"
     CDS             152..1237
                     /locus_tag="VIL39_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEY5481288.1"
                     /translation="MNTHLLKFSRRDFLWTAAAFTASTAVLRSQQPPAEVPQAASPPG
                     RRSVVAVSNGASRRQNIAEALLAIEDQIMPVLKTKKHVVIKPNIVSTNNQLASTHLDS
                     LHGILDFLAPRFKGPVIIAESSADYTTEGYNNFGYVKLAPEHKPLAVSLVDLNEEGKF
                     QTHLILNGDLHLVPVRLAARLLDPDAYIICAAMLKTHNTVVATLSLKNMALGAPLHSP
                     RKESRRWNDKRVYHGGVRQTHVDIALTAQRLQPFWGATVLDGWEGMEGNGPTEGHLVS
                     SRLAIASTDYVAADRVGLEVMGINPEWVGYLGFCAGAGLGQSSLPNIEIRGTKLAAVT
                     KKYQMHGDLERELRWQGPMKEIPEKLG"
     gene            1422..>2948
                     /locus_tag="VIL39_07405"
     CDS             1422..>2948
                     /locus_tag="VIL39_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481289.1"
                     /translation="METANLRSDVAIVYGIDPGLPKRIETWRNRGYRIHVMTGVSWGN
                     YQDYLYGRFDGINYEDEAQTERNGKMVSHGGDVYYMCPGTNYGKFLCLGVQRALDAGA
                     EAIHLEEPEFWVKSGYSEGFKREWRAYYGEDWQPPHSSVDAQWRASKLKYFLYRRALQ
                     QVFDHIQDYNRRTGRKVRCYVPTHSLLNYAQWGIVSPESSLARLNGCDGYIAQVWTGT
                     SREANVYRGKLRQRTFETAFLEYGAMQNLVRATGRRVWYLNDPIEDNPNYDWDDYRVN
                     WESTLVASLLQAEVSHYEVAPWPERIFGGRYPRNAAAGVRRGIPPAYATELQTVMNAL
                     NDMDQRRVEWNGGSTGFGLVVGDALMFQRGEPTPSNPRLSNVYGLAMPFVKRGIPLTP
                     VQLENLTVAHYLKGFSVLLLSYNGMKPQTPEVHAPLADWVRRGGVLVVVDDDADPYNH
                     VREWWNSNGFAYATPRAHLFKALGISDNDFVAGDKSVKVGKGYVRWLRENPAALAASE
                     T"
BASE COUNT          598 a          951 c          868 g          531 t
ORIGIN      
        1 tggccgaaac gacggccttc accacgccgg tcgtgccagg ctcataccgc gtctttgccg
       61 ttgaatagca gtccacccca ccgcgagaga cagcgaaatc acctcatcgg cagaattgac
      121 tccgatgaag aaacctctaa accttagccc catgaacacg cacttgctga aattctcacg
      181 ccgagacttc ctctggaccg cggcggcgtt caccgcctcc accgccgtgc tccgctccca
      241 gcaaccaccc gccgaggtcc cccaggccgc cagcccgccc gggcgacggt cagtggttgc
      301 cgtttcgaat ggggccagcc gccgccagaa cattgctgag gcgttgctgg ccatcgagga
      361 ccagatcatg cccgtgctca aaaccaaaaa gcacgtcgtc atcaagccca acatcgtcag
      421 caccaacaac cagcttgcct ccacccattt ggattccctg catggcatcc tggattttct
      481 cgcgccgcgc ttcaaggggc cagtgatcat tgccgagtcc tccgccgact acaccacgga
      541 aggatacaat aatttcggct acgtcaaact cgcgccagag cacaagccgc tggctgtcag
      601 cctggttgat ttgaatgagg agggcaaatt ccagacccat ctcattttga atggcgatct
      661 ccacctggtg ccggtccggc tggcggcccg cctgctggac ccggacgcct acatcatttg
      721 cgcagccatg ctcaaaaccc ataacaccgt ggtcgccacg ctttcgctca agaacatggc
      781 cctgggcgca cccctgcata gtccccgcaa agagagccgg cgctggaatg acaaacgggt
      841 ttaccacggc ggcgtccgcc aaacccacgt ggacatcgcg ctcaccgccc agcggctgca
      901 gcccttctgg ggcgcgaccg tgctggatgg ctgggagggc atggaaggca acggcccgac
      961 cgagggccac ctggtgtcct cccgcctcgc cattgcttcc acggactacg tcgccgccga
     1021 ccgcgtcggc ctcgaggtca tgggcatcaa tccggaatgg gtgggatacc ttggcttctg
     1081 cgccggcgct ggcttgggcc agagcagttt gcccaacatt gagattcgcg ggaccaagct
     1141 ggccgcggtg accaaaaaat accaaatgca cggcgacctc gaacgtgagt tgcgctggca
     1201 gggaccaatg aaggagatcc ccgagaagct cggctgattg ttccagtcca ttccaatcct
     1261 cattacgacc tgcacccacc attatgaccc gcctcccatt tgctgcggct tggttttgcc
     1321 ttgtaaccgc agttgcaccc gcgtcggccc agtcgctccc ctttcccccc gacccagccc
     1381 agtcgctgga gcgaacctgc ttccagacca gccggccttg gatggagacc gccaacctgc
     1441 gttccgatgt cgccatcgtc tatgggattg atccgggctt gcccaaaaga attgaaacct
     1501 ggcgaaatcg gggctatcgc attcacgtca tgaccggcgt atcgtggggg aactaccagg
     1561 attacctcta cggccgattc gacggcatca actacgagga cgaggctcag accgagcgga
     1621 acgggaagat ggtcagccac ggtggcgacg tctattatat gtgccccggg acgaactacg
     1681 gcaaattcct gtgtctgggc gtgcaacggg cgctggatgc gggggcggaa gcgatccatc
     1741 tcgaagagcc ggagttctgg gtgaaaagcg gctactcgga ggggttcaag cgcgagtggc
     1801 gcgcgtacta cggcgaggac tggcagccgc cccatagctc ggtggacgcc caatggcgcg
     1861 cctcaaagct caaatacttc ctttatcgcc gcgcgctgca gcaagtcttt gatcacatcc
     1921 aggactacaa tcgacgcacg ggtcgcaagg tccgctgcta cgtgcccacg cacagcctgc
     1981 tcaactacgc ccagtgggga atcgtcagcc ccgaatccag tctggcgcgg ctcaatggct
     2041 gcgacggcta tatcgcccag gtctggaccg gcacttcgcg cgaggccaac gtttaccggg
     2101 gcaagctgcg gcagcgcacc ttcgagaccg cctttctgga atacggagcg atgcagaacc
     2161 tggtgcgggc aacgggccgc cgggtgtggt acttgaacga tccaatcgaa gataacccca
     2221 attatgactg ggacgattac cgtgtgaatt gggaatccac gctggtggcg tcgctgctgc
     2281 aggccgaggt ctcgcactac gaagtggcgc cctggccgga gcggattttc gggggccgct
     2341 acccgcgcaa tgccgccgcc ggggtgcgcc gtgggattcc gccggcctac gcgaccgagc
     2401 ttcagacggt gatgaacgcg ctcaacgaca tggaccagcg gcgggtggaa tggaacggcg
     2461 gcagcaccgg cttcggcctg gtggtgggcg atgcgctcat gttccaacgc ggcgaaccga
     2521 ctcctagcaa tccccgattg agcaacgttt acggcttggc gatgcccttc gtgaaacggg
     2581 gcattccgct gacgcccgtc caactggaga acctgaccgt cgcccattat ctcaaaggct
     2641 tcagcgtgct gctgctgagc tataacggga tgaagcccca aacccccgag gtgcatgcgc
     2701 cgctggcaga ctgggtccgg cgcggcgggg tgctggtcgt cgtagatgac gacgctgatc
     2761 cctacaacca cgtccgtgaa tggtggaaca gtaatgggtt cgcctacgcg actccgcgag
     2821 cacacctctt taaagcgctc ggtatcagcg ataatgactt tgttgccggc gacaaatcgg
     2881 taaaagtcgg caaaggctat gtgcggtggc tgcgagagaa tcccgcggcg ctggcagcca
     2941 gcgaaacc
//
LOCUS       DATCGS010000362         7821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_180654, whole genome shotgun sequence.
ACCESSION   DATCGS010000362 DATCGS010000000
VERSION     DATCGS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180654"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="pgi"
                     /locus_tag="VIL39_07410"
     CDS             <1..971
                     /gene="pgi"
                     /locus_tag="VIL39_07410"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY5481290.1"
                     /translation="RAWLLQGLGGNKQAVAKHFVAVSTSAERVSAFGIDTANMFAFWD
                     WVGGRYSMCSAIGLSTMLAIGPENFRAMLDGFHRMDEHFRTEPFDRNLPVLMALLGIW
                     NTDFFGAQTVAVLPYEQYLKRFPAYLQQLTMESNGKHVTLAGTTVDYDTSAVYWGEPG
                     TNGQHSFYQLIHQGTRLIPCDFIAFAQALTPLGRHHDMLLANVFAQSEALAFGLTPEQ
                     VKAEGVPDWLVPHRVFEGNRPSNTLFADRLTPETLGKLVALYEHIVFTQGAIWNIGSF
                     DQWGVQLGKVLAQRIVPELESKTASKLKHDSSTNNLIRRYRKMREA"
     gene            981..1670
                     /gene="pgl"
                     /locus_tag="VIL39_07415"
     CDS             981..1670
                     /gene="pgl"
                     /locus_tag="VIL39_07415"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY5481291.1"
                     /translation="MEIRVLADAATVAAEAAKVIAEEGRGAVAARGRFSLAVSGGQTP
                     WQMLRALAREDVAWNSVYVAQVDERVAPAGHPDRNLTHLRDALLERVPLRPEQICAMP
                     VESPDLEAAASRYALMLQEIAGSPPVLDLIHLGLGPDGHTASLVPGDPVLDVTEADVA
                     LTGLYQQRRRMTLTYPMLNRARRILWVVTGGEKAGMLMRLRDGDVTIPAGRVCRDHAL
                     VLADRAAAQSR"
     gene            complement(1859..2785)
                     /locus_tag="VIL39_07420"
     CDS             complement(1859..2785)
                     /locus_tag="VIL39_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5481292.1"
                     /translation="MSKLSRREFIGRTLAASASIPLCAGSALSGLAAETFSPPIVVFS
                     KVYQTLKLNFDEAAALTAEAAIDGIDCPVRPGGEVLPEHVARQLPEYAAALRKRGLQL
                     PLLTTAITGVSSPCAEDILRTAKKLGVQFYRLGFVIRETNASAGKQVREIKAQLKDLA
                     ALNKQIGIGAIFQNHSPAGQTYVGGDLAELYDIVKDFDPAQIGVAFDIGHALVVHGDD
                     WRRHFEKLKPHLKIAYVKDVKREGRWVPFGEGDIGSTGYFKLLKELKYQAPVSLHIEF
                     DWTDGGKSKTRPALVKALQDSSRVLRRWLASA"
     gene            complement(2778..4799)
                     /locus_tag="VIL39_07425"
     CDS             complement(2778..4799)
                     /locus_tag="VIL39_07425"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY5481293.1"
                     /translation="MKKLITCLMSLVACLPLWAAEQEFTYVDLVKRLTDLEQLAVTPV
                     PGEQCAQCSSYDRASRYDTATGKYVNWDANGDGDGIIRKEGDKLVLAEMEGPGCIFRT
                     WSAAPKDGHVRIYLDGASEPAVDLPFVGYFDRKNAPFTRDALVHTVGMGWNNYTPIPY
                     QKSCKVVADPGWGQYFQFTYRTFPKGTQVPTFKRDLAPAELAALDQANAVLSQGGFGV
                     GTQPSSEKLWSKAVKAPAGKTTAVTRLKGPQAITGIRVKLDQPPAPGDLEALREVALQ
                     IKWDGEATPSVWAPLGDFFGTAAGVNHYRSLPLGVGEDGWWYCNWYMPFAKEARVELI
                     NDGKTQRGVKFEVTYAPLSQPIGKLGRFHAKWHRDAFLPREPERAIDWPLLKTEGAGR
                     FVGVMLHVWNPRGGWWGEGDEKFFVDGEKFPSTFGTGSEDYFGYAWCCPQLFQNAYHN
                     QTHNDGNNRGHVSVNRWQISDNVPFQTSFEGCIEKYYANNRPTLYAGMAYWYVTAGGN
                     DPYRPAPVSERVGYWTAVQSVQVKGAIEGEKLKILAKTGGNTQEQDMTGFPGQWSNDA
                     HLWWIGTKPGDKLDLALPVKETGKYQLGAQLTKAPDYAIVQLYLDGQKLGSPIDLYDK
                     SVIPSGLLALGACDLTAGDHKLTVEILGANDKATKNYMFALDYVKLTHE"
     gene            complement(4875..6305)
                     /locus_tag="VIL39_07430"
     CDS             complement(4875..6305)
                     /locus_tag="VIL39_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481294.1"
                     /translation="MKTEKQSMFVTADGKNVLFTFTIVSSLFLLWGFCNGMIDVMDKH
                     FQEELHLNLSQSAWVQFAHYLGYFLMSLPAAWLATKLGYKGGIIAGLLLVAVGGFWFI
                     PATKIAAFWAFLLGVCIIASGLTFLETVANPYTTVLGDPRYAATRINLAQSCNGVGWI
                     FGPIAGGMFFYGKDSAGNSTGSQTLWIPYAGVAVVVILLAVVFYFANVPDIKTEDDYR
                     LDDSAPGTSHSIWSHPHFVMAVAAQFFYVAAQAGIFSFIINYMTSQPPAIPASWNGAL
                     TNASASVQHFVAQLPGWIAWSVSWVPGWLTGWFETNKSGVLCFTNKAGANLASLGFVC
                     FLIGRATGAGMLRRVSAHKMLGLYGLLNVVVCLLVFAKLGWLSVACVFVSYFFMSIMF
                     PTIFALGIFGLGVRAKRASAYIVMAIMGGALLPKLMGAVADKYDISRGFIVPMFCFVF
                     IAFYGYYWPKLSNAKSLQEVPTSGGH"
     gene            complement(6422..7645)
                     /locus_tag="VIL39_07435"
     CDS             complement(6422..7645)
                     /locus_tag="VIL39_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008842939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6786 family protein"
                     /protein_id="HEY5481295.1"
                     /translation="MKRNSIIATILVMSTTASLTPRAGAASFGDDAAFLKSHTDLIVL
                     SDKKGLAKVAVAPAWQGRVMTSTAGGDAGISFGWINREFIAAGKLVPHMNAFGGEERF
                     WLGPEGGQFSIFFPKGAKFEFADWQTPPPIDSLPFKVVGQSKAQAEFAAKFALTNYSG
                     TRFEVAVDRAVRLLDTAAAWKKLGVRPAQDVTLVAFESDNKITNVGKNAWKKDTGLLS
                     IWILGMFNPSPSATIVAPIKPGPESDLGVKVTSDYFGAIPADRLVVKDNVIFLSADGK
                     CRSKIGLNPKRSKAVLGAYDADSKVLTIAQFNQPADVTDYVNSLWKLQNNPYGGDAAN
                     SYNDGPATPGAKPMGPFFEIESSSPAAALAPGKSLSHIHRTIHLSGPESALDSVARAT
                     LGVSLAEIKAAFKNQ"
BASE COUNT         1504 a         2364 c         2436 g         1517 t
ORIGIN      
        1 cgcgtgcttg gttgctccag ggcctgggcg gaaacaagca ggctgtggcg aagcatttcg
       61 ttgcggtttc gaccagcgcc gagagagtgt ccgcgttcgg catagacacc gccaacatgt
      121 tcgccttttg ggactgggtg ggcgggcgct attcgatgtg ctcagcgatt ggcctttcaa
      181 ccatgctggc catcggcccg gagaacttcc gcgcgatgct cgacggcttt caccggatgg
      241 acgagcactt ccgcaccgag cccttcgacc ggaacctgcc ggtgctcatg gccctgctgg
      301 gcatctggaa caccgacttc ttcggcgcgc agaccgttgc ggtgctgccc tacgagcaat
      361 acctgaaacg gttcccggcc tacctgcaac agttgacaat ggagagcaac ggcaagcacg
      421 tcacgctggc tgggaccacg gtggactacg acacgagcgc cgtttattgg ggcgaaccgg
      481 gcaccaacgg ccagcactcg ttctaccagt tgattcacca ggggacccgg ctcattccgt
      541 gcgacttcat tgcctttgcc caggcgctca cgccgctggg gcgtcaccac gacatgctgc
      601 tggctaacgt gttcgcgcaa agcgaggcgc tggccttcgg cctgacgccg gaacaggtca
      661 aagccgaggg cgtgccggat tggctcgtgc cgcaccgggt tttcgagggc aaccgcccat
      721 ccaacaccct cttcgccgac cggctcacgc cggaaacgct aggcaagctg gtcgccctct
      781 atgagcacat tgtcttcacg cagggcgcca tctggaacat cggctcgttc gatcagtggg
      841 gcgtgcaact gggcaaggtg ctggcccagc gcattgttcc ggagcttgag agcaagactg
      901 cgtccaaact caaacacgac agctcaacga acaacctgat ccgccgctac cggaagatga
      961 gagaagcgta ggccggcgcc atggagattc gagtgcttgc ggatgctgcg acggtcgccg
     1021 ccgaggcggc caaggtcatt gccgaagagg gcaggggagc ggtggcggca cgcgggcgct
     1081 tcagcctggc cgtcagcggc ggtcaaaccc cttggcaaat gctgcgcgca ctcgcgcgcg
     1141 aggatgtcgc ctggaacagc gtgtacgtgg cgcaggtgga tgagcgtgta gcgccggcgg
     1201 ggcatccgga ccggaacctc acccacttgc gcgacgctct gctggagcgt gtcccgcttc
     1261 gccccgagca gatttgcgcc atgccagtcg aatctcctga cctcgaggct gccgcctcac
     1321 gctacgcgct catgcttcaa gagatcgccg gctcaccgcc ggtgctcgac ctgattcacc
     1381 tggggcttgg ccctgacggc cacacggcct cgttggtgcc tggcgacccg gtgcttgacg
     1441 tcaccgaagc agacgtcgca ctgacgggtc tttaccagca aaggcggcgg atgacgttga
     1501 cttacccgat gctcaatcgt gcgcggcgta tcttgtgggt ggtaacgggc ggcgagaagg
     1561 ccgggatgct gatgcgcctg cgcgatggcg acgtgacgat tccggccggg cgagtttgcc
     1621 gggaccacgc gctggtgctc gccgaccgtg cggcagcgca aagccgctaa cagcctgaga
     1681 ggagagaatc ctcccgcgct tttgacgcgc cctgcgcccc tgcactggat gggcagggta
     1741 gtggggcggg tgtctcgcct gccatcaggg cgtcctgccc tcgaactctc caccacgggc
     1801 gagacgcccg gtgcggcagg cgagacgccc gccccactac ctggcgggtc tcatgggctt
     1861 acgcagacgc gagccagcgt ctcagcaccc gcgagctgtc ttgcagcgcc ttgaccagcg
     1921 ccgggcgcgt tttggacttc cctccgtcgg tccagtcgaa ctcgatgtgc aatgagaccg
     1981 gtgcctggta cttcagctcc ttgagcagct tgaaatagcc cgttgagccg atgtcacctt
     2041 cgccaaaggg cacccagcgt ccctcccgct taacgtcctt tacgtaggcg atcttcaggt
     2101 gcggtttcag cttttcgaaa tgccggcgcc aatcgtcccc atgcaccacc agcgcgtggc
     2161 cgatgtcgaa ggcgacgccg atctgcgccg ggtcaaagtc cttaacgatg tcatacagct
     2221 cagccaggtc gccgccgacg taggtctgcc cggcgggaga atgattctgg aaaatagcgc
     2281 ctatgccgat ctgcttgttg agtgcggcca ggtctttgag ctgggcctta atttcgcgca
     2341 cctgcttgcc ggctgacgcg ttggtttcgc gaattacgaa tcccagacgg tagaactgca
     2401 cgcccagttt cttagccgtg cgcagaatgt cttcggcgca gggcgaagag acacctgtga
     2461 tcgcggtcgt caacaaaggt aactgcagac cccgcttcct cagggctgcg gcgtattcgg
     2521 gcaactggcg ggcaacgtgt tccggcagaa cctccccgcc gggacgcacc gggcagtcta
     2581 tgccgtctat cgccgcttcc gccgtaagcg ctgccgcttc atcgaagttc agcttcagcg
     2641 tctgatagac tttgctgaag accacaatag gcggcgagaa ggtctccgcg gcgaggcccg
     2701 agagggcgct gccagcgcac aaggggatgc tcgcggacgc tgccagcgtt cggccgatga
     2761 attcgcggcg ggagagctta ctcatgagtg agcttcacat agtccagggc gaacatgtag
     2821 ttcttggtcg ccttatcgtt cgcgcccagg atttcgaccg tcagcttgtg atctcccgct
     2881 gtcaggtcgc aagcccccag agccagcagg ccgctgggga ttacggactt gtcgtagagg
     2941 tcaatggggc tgccgagctt ttggccgtca aggtagagtt gcacgatggc gtagtcgggt
     3001 gctttggtca gttgcgcgcc cagttggtat ttgccggttt ccttgaccgg caacgccagg
     3061 tccagtttgt cgcccggctt ggtcccaatc caccacaaat gcgcatcgtt gctccattgg
     3121 cccggaaaac cggtcatgtc ctgctcctgc gtgttgccgc cggtcttggc caggatcttc
     3181 agcttctcgc cctcgatcgc gcctttgacc tggacggatt ggaccgccgt ccagtagccg
     3241 acgcgttcgc tcacgggcgc ggggcggtag gggtcgttgc cgcccgcggt cacgtaccag
     3301 taggccatgc ccgcatagag cgtcggacgg ttgttggcgt aatacttctc aatgcaccct
     3361 tcaaatgacg tctggaaggg cacgttgtcg ctgatctgcc accgattcac ggacacgtgg
     3421 ccacggttgt tgccgtcgtt gtgcgtctgg ttgtgatacg cgttctggaa gagttgcggg
     3481 cagcaccagg catagccgaa gtaatcctcc gaccccgtgc cgaaggtgga gggaaacttc
     3541 tcgccatcta cgaagaactt ctcgtcgccc tcgccccacc atccgccgcg tggattccag
     3601 acatgcagca tcacccccac gaaccggccc gcgccctccg tcttcagcaa cggccagtca
     3661 atcgcgcgct cgggttccct cggcaggaat gcgtcccgat gccacttggc gtgaaaacgc
     3721 cccagcttcc cgattggctg cgacagcggc gcataggtta cctcgaactt cacgccccgt
     3781 tgcgtcttgc catcgttgat cagttccacg cgcgcctctt tcgcgaacgg catgtaccag
     3841 ttgcaatacc accagccatc ctcacccacg cccagcggca acgaacggta atggttcact
     3901 ccggcggccg tgccaaagaa atcgccgagc ggcgcccaga cgctcggtgt cgcctcaccg
     3961 tcccatttga tctgcaaggc cacttcgcgc agcgcctcga gatctccggg ggcaggcggc
     4021 tggtcgagtt taacgcgtat gccggtgatg gcctgcgggc ctttcagtct cgtgacggcg
     4081 gtggtcttgc ccgcgggggc cttcaccgct ttgctccaca gcttctcgct ggagggctga
     4141 gtcccaaccc cgaaaccgcc ctggctcagc acggcattcg cctgatctag cgcggccagt
     4201 tccgccggcg ccagatcccg cttgaaggtc ggtacttgcg tgcccttggg aaaggtccga
     4261 tacgtgaact ggaaatactg accccagccc ggatcggcga caaccttgca ggacttctgg
     4321 tagggaatgg gcgtgtaatt gttccagccc attccgaccg tatgcacgag cgcatcgcgg
     4381 gtgaacggcg cattcttccg atcgaaatac ccgacaaacg gcagatccac cgccggttcg
     4441 ctcgcgccat ccaggtaaat acgcacatga ccgtctttgg gcgcggcgga ccacgtccgg
     4501 aagatacagc ccgggccttc catttcggcc aggacgagct tgtcgccttc cttgcggatg
     4561 atgccgtcgc catcgccgtt ggcatcccag ttcacatatt tgccggtcgc ggtatcgtag
     4621 cggctcgcgc ggtcgtagct cgagcactgg gcgcactgtt cgcccgggac cggggtaacc
     4681 gccaactgct ccaggtcagt cagccgctta accagatcaa cgtaggtaaa ttcttgttcc
     4741 gccgcccaaa gcgggaggca ggcaaccaaa gacatcagac aagtgatgag tttcttcata
     4801 gcaattaata gcaaatgctg tttacggact tatccgaagt ggtgttcgat gccgattcgg
     4861 ttccacagtt acgatcaatg gccgccggac gtggggactt cttgcaacga cttcgcattg
     4921 ctcagcttcg gccagtagta gccatagaag gcgataaaga cgaagcagaa catcgggaca
     4981 atgaacccac gggagatgtc gtatttgtcg gcgaccgcac ccatgagttt gggcaagagg
     5041 gcgccgccca tgatcgccat gacgatgtag gcggaggctc ttttcgcccg gacacccaac
     5101 ccaaaaatgc cgagggcaaa gatggtgggg aacatgatgg acatgaagaa gtagctcaca
     5161 aagacacagg cgactgatag ccagccgagc ttggcgaaga ccaagaggca taccaccacg
     5221 ttgaggaggc cataaagccc cagcattttg tgcgcggaaa cccttcgcag catgccggcg
     5281 cctgtggcac gaccgatcag gaagcagaca aagccgaggg aggctaggtt ggcgccagcc
     5341 ttgttggtga aacagagaac accgctcttg ttggtctcga accagcccgt cagccagccg
     5401 ggcacccaac tgaccgacca cgctatccaa cccggtaact gcgccacgaa atgctggacg
     5461 ctggccgagg cattggtcag ggcgccattc caagaggcgg gaatggccgg gggctgtgag
     5521 gtcatgtagt tgatgatgaa gctgaaaatg ccggcttggg cagccacgta gaagaactgc
     5581 gcggcgaccg ccatgacaaa gtgcggatgc gaccagatgg agtgggaagt cccgggcgcg
     5641 gaatcgtcga ggcgataatc atcctcggtc ttgatgtccg ggacatttgc aaagtagaag
     5701 acgacagcaa gaaggatgac caccaccgca acgccggcgt aaggaatcca aagcgtctgg
     5761 ctgccggtgc tgttgccggc cgaatccttg ccatagaaga acatcccacc ggcgatgggg
     5821 ccaaaaatcc agccgacgcc attgcaggac tgggctagat tgatacgggt ggcggcatag
     5881 cgtggatcac caagcaccgt tgtgtaaggg ttggccacgg tttccaggaa tgtgaggccg
     5941 gaagcaataa tgcagacgcc gagcagaaat gcccagaagg ccgcaatctt cgttgctgga
     6001 ataaaccaaa aacctcccac cgcgaccagc agcagaccgg caatgatgcc ccctttgtat
     6061 cccagtttcg tcgccaacca agccgccggg agcgacatca ggaagtaccc caggtagtgg
     6121 gcgaattgta cccaggcgga ttgcgatagg ttaagatgca gttcctcctg gaaatgcttg
     6181 tccatcacgt caatcatccc gttgcagaat ccccagagga gaaacagcga gctgacgatg
     6241 gtgaaggtga acaggacgtt cttcccgtcc gcggtgacga acatgctttg cttctctgtc
     6301 ttcatatatg tggcgcgatt gcttagggtg taggttccga tgctgtctgg cagggacgtt
     6361 tcagcgaaac atccctgcct cgctgggttg aagttgcgaa ctcggtgcag cggcgtgcat
     6421 gtcactgatt cttaaacgcc gctttgatct ctgccaggga gactcccagc gtggcgcgag
     6481 cgacgctgtc cagcgcggat tctggtccgc tcagatggat ggtgcgatgg atgtgcgaga
     6541 ggctcttgcc aggcgccagg gcggcggccg gggaggagga ctcgatttcg aagaagggcc
     6601 ccatcggctt ggcgccgggg gtggccggtc cgtcattgta gctgttggcg gcatcgccac
     6661 cgtaggggtt gttctgcagc ttccagagcg aattcacgta gtcggtgacg tcggcaggct
     6721 ggttgaattg cgcaatggtg agcaccttgc tgtcggcatc gtaagcgcca aggacggctt
     6781 tgctgcgttt agggttgagg ccgatcttgc tgcggcattt gccgtcggcc gacagaaaga
     6841 tgacgttgtc tttgaccact agccgatccg ccggaatggc gccgaagtaa tcggacgtca
     6901 ccttcacgcc gagatcggac tcagggccgg gcttgatcgg ggcgacaatg gtggccgagg
     6961 gcgaggggtt gaacatgccg aggatccaga tcgaaagcag tccggtgtct ttcttccacg
     7021 cgttcttgcc gacattggtg atcttgttgt cagactcgaa ggcgacgagg gtcacatcct
     7081 gggcgggccg cactcccagc ttcttccaag ccgcggcggt atcgagcagg cgcacggcgc
     7141 ggtccacggc gacctcgaag cgagtgccgg agtaattcgt cagagcgaat ttggcggcga
     7201 attcagcctg ggcctttgac tggccgacga ccttgaaggg caggctgtca atggggggcg
     7261 gcgtctgcca gtcggcaaat tcgaacttgg cgcccttggg gaagaagatc gagaactgcc
     7321 cgccttcggg tcccagccag aaccgctcct caccgccgaa ggcattcatg tgcgggacga
     7381 gcttgccggc ggcgatgaac tcgcggttga tccagccgaa gctgatgccg gcatcgccac
     7441 ccgccgtgct ggtcatgacg cggccctgcc aagcgggcgc gacggccact ttggccaggc
     7501 ccttcttatc gctcaatacg atgaggtctg tgtggctctt tagaaatgcg gcgtcgtcac
     7561 caaaactggc ggcgccagca cggggcgtga gagatgcggt tgtgctcata acgagaatcg
     7621 tggcaataat agaattccgt ttcatgatcg tgagcctacg gattgacgcc gggcgttgtc
     7681 aagcgttcgg gattggttgg gggcgccgta tctcggcacc gccatcggtg cgcggacatt
     7741 cctgtccgca gcaagttccg aaaggcaacg cgcttcggcg agtttcccaa ccattttacc
     7801 catcgaacgt tgctgcggac a
//
LOCUS       DATCGS010000363        12030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_180682, whole genome shotgun sequence.
ACCESSION   DATCGS010000363 DATCGS010000000
VERSION     DATCGS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180682"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VIL39_07440"
     CDS             complement(<1..1146)
                     /locus_tag="VIL39_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015139304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEY5481296.1"
                     /translation="MNSNQTSPVATTERSPSAPEPRRAPRSPTRLPELLAPAGDWECA
                     RAAVENGADAIYFGLEKFNARMRANNFTEADLPKLMEFLHRRGVKGYVTFNILVFENE
                     MADARQYVRAMIAAGMDAAVVQDVGMCRLIRQHSPDFPIHVSTQMTITSAAGVEFARD
                     LGCNLVVLARECSLKEIEALQSSQLAGYQLSTLNHPLPLEVFIHGALCVAYSGQCLTS
                     EALGGRSANRGECAQACRMPYELVADGKLVLLGDRKYLLSPQDLAGLEVLPELARIGV
                     ASLKIEGRLKSPEYVANITRIYRKALDRVCDAGCGMRDQTQPHAPASPNYQSPITHQD
                     QYDMEMAFSRGLYTGWFHGTNNQQLVHGRFGKKRGVFLGEVSRIEGER"
     gene            1272..1352
                     /locus_tag="VIL39_07445"
                     /pseudo
     CDS             1272..1352
                     /locus_tag="VIL39_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            1593..2177
                     /locus_tag="VIL39_07450"
     CDS             1593..2177
                     /locus_tag="VIL39_07450"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5481297.1"
                     /translation="MNTSILKWTGGAWALVAGMTLAAVAGPSLKVGDPAPKLQTGKWV
                     QGEPVKDFQKGKAYIVEFWATWCPPCRASIPHLNETYNKFKDKGLIVIGQDCSEQNDA
                     LVAPFVKKMGDKMTYRVALDDKATNKQGQMSKTWMEAAGRDGIPSAFLVDTKGVIAWI
                     GHPMELKEQVIEEVLAGTFDVRKAAAAPKTQRRD"
     gene            2397..3062
                     /locus_tag="VIL39_07455"
     CDS             2397..3062
                     /locus_tag="VIL39_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481298.1"
                     /translation="MAKRKKNAAAVALGRLGGWWGVKRVAQLVLGWLVVGGALAGVAG
                     DVESYANRISSLVAPAKLATLGVRGANSRVQKYVAILAEAKADGVAPKKVAGKAVAVV
                     GMKGKAAKLTGEAMVRNLTIAERLGCLDSEGLGEMRRGQAPTVRRGPYRGEKLSVDHI
                     IPRTVVPELDNLIANLELMPLKMNEGKNAMIGARQVDLARKLHKAGLLSSGGLRAVER
                     GGK"
     gene            complement(3083..4651)
                     /locus_tag="VIL39_07460"
     CDS             complement(3083..4651)
                     /locus_tag="VIL39_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY5481299.1"
                     /translation="MAFDSFRAFLNRLETAGDLRRITQPVATELEITEIADREMKKPG
                     GGQALLLEKPTVNGQISPFPLAINTLGSDRRMAMSLGANSIDELAADLGSLVKAKPPA
                     SFKEALSLLGTALDLRHAKPKVVKDGPCKEVIHKFEAPPTRTEPWPKAPDILKVGPAS
                     GLSPAAQDSKEHRGAFPTLLNLPIQQCWPLDGGRFITLPGVVTRDPDTGERNVGMYRM
                     QVYDERTTGMHWQMQKVGARHGRRYYETGTRMPVAVFLGGDPVFPFCATAPLPDGLDE
                     FMLAGYLRKQATELVRCETNDLEVPANADFVLEGYVDPKEPLREEGPFGDHTGYYSLP
                     DLYPVFHLTAITHRKDAVYPATIVGMPPMEDFYIGSASLKLFLPILKMTFPELVDLAL
                     PAEGVFHNLVFVSIKKTYPMQAFKIMHGLWGMGQMMFTKYIVVVDDDVDVHNSREVLF
                     RLCANTDPQRDAIFTKGPADVLDHATSELGVGTKMGIDATHKLPGEGHKRGWPPLIRM
                     DEAVKQKINQLCSR"
     gene            4904..5137
                     /locus_tag="VIL39_07465"
     CDS             4904..5137
                     /locus_tag="VIL39_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481300.1"
                     /translation="MKIKANRKKALPALANGQLWKTENAYIQIVELGKRLIDYRMMRE
                     LGQARRTQTSSIETMEKYLKTNEGRLVKGNSRN"
     gene            5327..6316
                     /locus_tag="VIL39_07470"
     CDS             5327..6316
                     /locus_tag="VIL39_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5481301.1"
                     /translation="MRSVKSPRNNNACNPQSQARMKALALILPALVFMAALLGGCGGH
                     QESTVVVYCAQDQVFAEPLLAEFTRQTGIKARTIFDSEAVKTVGLANRLLAEKDHPQC
                     DVFWGNEELRTRQLAAHGVFRATNGFKTFGFRSRRIVINTNKVSLTEAPRTLVELTNA
                     TWRGKVALAYPMFGTTATHFLALRQRWGDAPWQTWCRALQANKPFLVDGNSVVVKLVG
                     QGEAWIGLTDSDDVAGGIREGLPLAAMPLTPETLLIPNTVAVVRGAPHAAEAEQMFQF
                     LQRSEMAERLVKEQALEGAQASQPQGLQVDWEALLHDLDAATAIMRDIFLRRQ"
     gene            6375..7349
                     /locus_tag="VIL39_07475"
     CDS             6375..7349
                     /locus_tag="VIL39_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate-resistant acid phosphatase type 5
                     family protein"
                     /protein_id="HEY5481302.1"
                     /translation="MIRSKFRPCSVQTRYGKLAIALTLTLLLLSPLQLFAQSVAPQQS
                     AGSLNFLVISDWGGKGGTAQVAVAKQLGLTAAAQKSSFVVTCGDNYHGSGISGAESPR
                     WKTEYEDIYSNPSLMIPWYPSLGNHDNRGRVEAEIAYSKISPRWKMPARYYTHTEKID
                     GTNEALFVHLDTSPFVTAYRKKGSSYRVAGQDPKVQLRWLDSVLGASRARWKIVIGHH
                     PVYSAAPTHGDTKELIADVLPLLQKYGVQVYFCGHDHVLQHLSHGGVNFFVCGGGSSH
                     RTVNQRNDVRFGAGSTGFLSMTLTAAAAQATYINDKGVELYRTSIPAP"
     gene            complement(7432..7836)
                     /locus_tag="VIL39_07480"
     CDS             complement(7432..7836)
                     /locus_tag="VIL39_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481303.1"
                     /translation="MNEKKRILVVDDEPSITRLLKLNLEQTGDYEVATENVSKAALAT
                     AEEFQPALILLDVMMPSLDGGTLASQLQISPKLKGVPIVFLTAAVTREEVRARRGLVG
                     GLPFLAKPVDLHEVLACLKRHLGAPQTPAPTP"
     gene            complement(7914..9596)
                     /locus_tag="VIL39_07485"
     CDS             complement(7914..9596)
                     /locus_tag="VIL39_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5481304.1"
                     /translation="MQTTVSIKEKNHAEQAAQESEQRYKRLLASVTDYVYSVTVDHGR
                     PTATAHGPGCEAVTGYSSREFNADPFLWYRMIYSEDRPTVTAQADRILNGETPPPLEH
                     RIIHKNGSIRWIRNTPVPHKNTLSRLIAYDGLVSDITERKRAELALRQSEERLALVIQ
                     GSSDGVWDWDLTTNEVYFSTRWKGMLGYDDHEVQNRFSAWEQLLHPDDRERAHARLQA
                     YLSGQTPTYELEHRLRDKDGSYRWILARGVALRDARGKPVRMAGSHVDLTERKQSEER
                     LERAYAELSQNEAALKTALQKLKTANEDLQTTQLQLIQAAKLESVGTLAAGVAHEVKN
                     PLQTILMGLDYLANNLPGTNGTTTMVLSDMRDAVTRANVIVRELLQLSAAKDFEPKEA
                     DLNDLVERSLWLINSQIIGAHISMVPKLGADLPRVMLDRGKMEQVFINLFLNALQAMS
                     EGGVLTVTTRQSRFGEDIKLSGPAFLQFKPGELVVIAEVQDTGKGIAEDHLPKIFDPF
                     FTTKPTGVGTGLGLSVVKKIIDLHGGAIDIQNAPLGGVVVTVVLRAEPEAKL"
     gene            10238..11083
                     /locus_tag="VIL39_07490"
     CDS             10238..11083
                     /locus_tag="VIL39_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEY5481305.1"
                     /translation="MIDEHPQWSRHGVAKALCQRWDWRTPTGRLKTFAARSLLLKLAQ
                     RQALRLPPVRAAMRRSPWGLGRPELRRASAPLPPLLEASLSRLQPLEWQIGGHGSPER
                     ERALTYLRHYHYLGCNRPVGTHLVYVVRDAQGRDLAVHLVGAAAWQCAPRDHYIGWSA
                     AERAAGLHRIGNHSRFLILPWVRVPHLASHLLGGLAQRLCVDWPVQHGWQLELLETFV
                     EAGRFKGVAYRAANWQPVGLTTGRTRQEKEHRPQAPRKSVWVYPLGERFRERLVARGS
                     HGGAR"
     gene            11080..>12030
                     /locus_tag="VIL39_07495"
     CDS             11080..>12030
                     /locus_tag="VIL39_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481306.1"
                     /translation="MSQPQARLPLVLEVFALLFSKQWIGQWQKKWCAAGRLTGSTSGH
                     PGRPNQTSASPPKPKQPAPGRFYERIFSLRVTLWYLIFQRLNFDQTQAAVVVNLREGG
                     ADRLGKQRKRKLSRRVRSTQTSAYNQARQRMPLELLAEALAHLRQGLLKLVGLVPAPR
                     QRPGPAERTRQILDGSTLAVLVTPLLARDYPPARNQSGTSDWSLMRIVVGFCARSGAV
                     LSALEGAMQRSEQAMAWTLIEAAAAFTLWVGDRNFGVWSVAAQAVRCHQDTLTRLTRA
                     RAAKLTRGQPMYSGEDRLIQWSPSRKDKVPPGTKRSDVSGR"
BASE COUNT         2329 a         3456 c         3883 g         2362 t
ORIGIN      
        1 gcgctcgccc tcgatccgcg acacctcgcc caggaagacg ccgcgcttct tgccgaagcg
       61 gccatggacg agctgctggt tattggtgcc gtgaaaccat ccggtgtaga gcccccgcga
      121 aaaggccatc tccatgtcat actggtcttg atgggtgatt ggcgattggt aattagggct
      181 ggcgggagcg tgcggctgtg tctggtcacg catcccgcat cccgcatcac acacccgatc
      241 cagggccttg cggtagatgc gggtgatgtt ggcgacgtac tcgggcgatt tgagccgccc
      301 ctcgatcttc aaagaggcca cgccgatacg agccagttcc ggcaggactt ccagcccggc
      361 caggtcttgc gggctgagca gatacttccg gtcgccgagc aggacgagct tgccgtcggc
      421 caccaactcg taaggcatgc ggcaggcctg cgcgcactcg ccgcgattgg ccgaacgccc
      481 gcccagcgcc tcgctcgtca ggcattgccc ggagtaagcg acacagagcg caccgtgaat
      541 gaagacctcc agaggaagtg gatggttgag agttgatagt tgatagccgg ctagttggga
      601 ggactgaagg gcttcaatct ccttaagcga acattcccgg gcgaggacga cgaggttgca
      661 gccgaggtcg cgggcaaact ccaccccggc ggcgctagtg atggtcattt gcgtcgagac
      721 gtgaatcggg aaatcaggcg aatgctggcg aatcaaccgg cacataccga catcctgcac
      781 gactgcggcg tccatcccgg ccgcaatcat cgcccgcaca tattgcctgg cgtcggccat
      841 ctcgttctcg aagacgagga tgttgaaagt gacatagcct ttcacgccgc gccgatgcag
      901 gaattccatc agcttgggca aatcggcctc ggtgaaattg ttggcccgca tgcgggcgtt
      961 gaacttctcc agcccgaagt agatcgcgtc cgccccgttc tcgacggccg cccgggcgca
     1021 ttcccaatcg cccgccggcg ccagcagttc gggcaagcga gttggcgagc gcggcgcgcg
     1081 gcgcggttcc ggggccgaag ggctccgctc cgtcgtggcc actggactgg tctgattact
     1141 gttcatcagc gaatcagaac ttaccctcta aactgcccgg gaacaaaggg ttattctatt
     1201 cgcatccctg ttgaacgaac cctcaaaatg ccggcttgaa accagcgccg aatccaattg
     1261 cctttgatcg catgaaactc tccgtcacag tggaccggga tgaggatgga atgtgggtga
     1321 tcgaatgtcc ctccattccc gggtgtgtta gccaccaagt tgtaagccag acgaatcact
     1381 tccagatcga gggcgttagc ggcgaagtcc cgagtgggga ttttgcgcat ggcgaaatct
     1441 tcccgcaatt cacagaggtg aggctccatg ccggctcgcc catcatagaa atgccagatc
     1501 ccagcaggag tgagcggtga actaagcgac tggggtgatt acctaccctt tccagcgcga
     1561 cttttgaccc gcaatggtgt aggatagccg gtatgaatac atcgatattg aaatggacgg
     1621 gtggcgcgtg ggctctagtc gcgggcatga cgctcgccgc ggttgccgga ccaagcctca
     1681 aagtgggtga tcccgcaccg aaattgcaga cgggcaaatg ggtgcagggc gagccggtga
     1741 aggatttcca aaagggcaag gcctacatcg tcgagttctg ggcaacgtgg tgtcccccgt
     1801 gccgtgcttc cattccgcac ctcaatgaga catacaacaa gttcaaggat aagggcctga
     1861 ttgtcatcgg ccaggattgc tcggagcaga acgacgctct ggtggcgccg ttcgtcaaga
     1921 agatgggcga caagatgacc taccgcgttg cgctcgacga caaagccacc aacaagcaag
     1981 gccagatgtc caagacctgg atggaagcgg ccggccggga cggcatcccc agcgcgttcc
     2041 tggtggacac caagggcgtc attgcctgga tcggccaccc gatggaattg aaggagcagg
     2101 tgattgaaga ggtgctcgcg ggaacattcg atgtgcgcaa ggccgcggca gctcccaaga
     2161 cgcaacggcg agactagcct ggatttccac ggaaaacagt tcgcctaacg tggtcctccg
     2221 aagaggctga tttctcttca aggtggattt gacaagcttt tcgcctgtag cgcggtggga
     2281 ggctgtcggg aacggcccga gcaggagcag gtttccccct ggaagcaact ggggccgtcc
     2341 agttgctggg ccagaagcgg cttccggcgg ggccgggttt gtggcatcag tcagggatgg
     2401 cgaagcgaaa gaaaaatgcg gcggcggtgg cgctggggcg tttgggcgga tggtggggcg
     2461 tgaagcgcgt tgcacagttg gtgctcgggt ggctcgtcgt tggcggcgca ctggccggcg
     2521 tggcgggtga tgtcgagagt tacgcgaaca ggatttccag cctggtcgct ccggcgaagc
     2581 tggcaacact gggcgtgagg ggggcaaact cgcgggtaca gaaatatgtg gcgatcctgg
     2641 cggaggcgaa ggcggacggg gttgcgccca agaaggtggc aggaaaagcg gtggcggttg
     2701 tggggatgaa gggtaaggcc gcgaaactga caggcgaggc gatggtgcga aacctgacga
     2761 tagcggagcg gttggggtgc ctggactccg agggactggg ggagatgcgc agaggccaag
     2821 cgccgacggt gcggcgcggg ccctaccgcg gcgagaagct gagtgtggac catatcattc
     2881 cgcggacggt ggtgccggaa ttggacaacc tgattgcgaa tctggagttg atgccgctga
     2941 agatgaacga ggggaagaat gccatgatcg gcgcgcggca ggtggatctg gcgcgaaagc
     3001 ttcacaaggc ggggctgctt agttcggggg ggctgagggc ggttgagcga ggcgggaagt
     3061 gagtttggct cggagccgtt catcagcggc tgcaaagttg gttgatcttc tgtttcaccg
     3121 cttcatccat ccggatgagc ggcggccagc cgcgcttgtg gccttcgccc gggagtttgt
     3181 gcgtggcgtc aattcccatc tttgtgccaa ctcccagttc actcgtcgcg tggtcgagca
     3241 cgtcggcggg gcctttggtg aagatggcgt cgcgctgcgg gtcggtgttg gcgcaaaggc
     3301 ggaagagcac ttcgcgggag ttgtgcacgt ccacgtcgtc gtccaccacc acgatgtatt
     3361 tggtgaacat catctgcccc atgccccaga gcccgtgcat gatcttgaag gcctgcatgg
     3421 ggtaggtctt cttgatgctg acgaagacca ggttgtggaa cacgccctcc gccggcagcg
     3481 ccaggtccac gagctccggg aaggtcatct tcaggatcgg gagaaacagc ttcaaggagg
     3541 cgctgccgat gtagaagtct tccatcggcg gcatcccgac aatggtggca ggatacacgg
     3601 cgtccttgcg atgcgtaatg gcggtgaggt gaaagacggg gtaaaggtcg ggcagcgaat
     3661 aatagcccgt gtgatcaccg aaaggtcctt cctcgcgcaa tggctctttg ggatcaacgt
     3721 agccctccag aacgaagtcc gcgttggccg gcacttccag gtcgttggtc tcgcaccgga
     3781 cgagttcggt cgcttgcttg cggaggtagc ccgcgagcat gaactcgtcc aggccgtcgg
     3841 gcaacggcgc ggtggcgcag aaggggaaca ccggatcgcc tccgaggaat accgccaccg
     3901 gcatgcgtgt gccggtctcg tagtaacgcc ggccgtggcg ggcgccgacc ttttgcatct
     3961 gccaatgcat tccggtggtg cgctcgtcat agacctgcat ccggtacatg ccgacattgc
     4021 gctcgccggt gtcggggtcc cgggtcacta cgcctggcag tgtgataaag cgcccgccgt
     4081 ccagcggcca gcactgttgg atgggcaggt tcaacaaagt aggaaaggca ccccggtgtt
     4141 ctttggaatc ctgcgccgcg ggagacaggc cggacgccgg tcccacttta agaatgtcgg
     4201 gcgctttggg ccacggttca gtgcgcgtgg gcggggcttc gaatttgtga atgacttcct
     4261 tgcatgggcc gtctttcacc accttcggct tcgcgtgccg caggtccagc gcggtgccga
     4321 gcaggctgag ggcttccttg aaactggccg gcggcttggc cttcaccagg gaaccgagat
     4381 cggcggccag ctcgtcaatt gaattggcgc ccaggctcat cgccatgcgc cgatccgaac
     4441 cgagcgtgtt gatcgcgagc gggaacgggg aaatctgtcc attgaccgtt ggcttttcca
     4501 gcagcagcgc ctggccgccg cccggcttct tcatttcgcg gtcggcaatc tcagtgatct
     4561 ccagctcggt ggctaccggt tgggttatgc gccgcaagtc accggccgtt tccagccggt
     4621 tcagaaaagc tcggaatgaa tcgaacgcca tgagcgaaga ttaccagacg aatggcgcca
     4681 agggaatcgt gattgcggat gagggaacga aaaggcggag tgatggcggg atgaagtatg
     4741 ggggtggtgg accatatggg agcccgccga gccccgacca cttgatcgag cttatcccgt
     4801 tcatcctgtc tcaaattgga ctcgcagcgg actgttctaa cccggatttc gggttccgga
     4861 gcctgaagag tgaacaaaac tgccttttag gcgtctaata gacgtgaaaa tcaaagcgaa
     4921 tcggaagaag gcgttgccgg cattggccaa tgggcagttg tggaagacgg agaatgctta
     4981 cattcagatc gtggagctgg gcaaaaggct gattgactac cggatgatgc gggagttggg
     5041 gcaggcgcgg agaacccaga ccagcagcat cgagaccatg gagaagtacc tcaagaccaa
     5101 tgaaggacgg ttggtgaagg ggaactcgag gaattgattt gtgccttcct cctgtccggc
     5161 tgccaagccg gagcccgggg aactaaggat ctttcattag cagcggttcc ccttgttgct
     5221 ggggaaaccc acttttcaat aacaaagcag acgctcccca atcgggctgc gccttggctg
     5281 tactgcccca aaccgcgcgc gggcggcaat tctcttgagc gaagcagtgc gatcagtaaa
     5341 atcgcccagg aacaacaatg cctgcaatcc acagtctcaa gcccgcatga aagccctcgc
     5401 ccttatcctc ccggccttgg tattcatggc ggccctgctg ggcgggtgcg gtggccatca
     5461 ggaatcaaca gttgtcgttt attgcgccca ggaccaggtt ttcgcggaac cgctgctggc
     5521 cgaattcacc cggcagaccg gtatcaaggc gcggacaatc tttgacagcg aggcggtcaa
     5581 aaccgttgga ttggcaaacc ggctcttggc cgagaaggac cacccgcagt gtgacgtatt
     5641 ctggggcaac gaagaactcc gcacgcggca actagcggct cacggagtct tccgggcaac
     5701 caacgggttc aagacgtttg gctttcgcag ccggcggatc gtcatcaata ccaacaaagt
     5761 ctcgctgacc gaggctcccc gcacactcgt cgaactgacc aacgcaacct ggcgcggcaa
     5821 ggtggcattg gcctacccga tgttcggcac gacggcgacc cacttcctgg ccctgcggca
     5881 gcggtggggc gacgcgccgt ggcagacgtg gtgccgggcg ttgcaggcta acaagccgtt
     5941 cctggtggac ggcaactcgg tggtggtcaa gctggtcgga cagggagaag cgtggatcgg
     6001 actgacggac tcggatgatg ttgcgggcgg aatccgcgag gggctgccgt tggcggcaat
     6061 gccgctgacc cccgaaacgc tcctgatccc gaataccgtg gccgtcgtgc ggggggcgcc
     6121 gcatgccgcc gaggcggaac agatgtttca attcctccag cgatccgaaa tggctgagcg
     6181 attggtgaaa gagcaagcct tggaaggcgc tcaagccagc caaccgcaag ggctacaggt
     6241 tgactgggaa gccctgttgc acgacctcga tgcggctacc gcaatcatga gggacatctt
     6301 cctgagacgg cagtaggaac ccgctctctc aaatctctgt aacaagccca aaccctgcga
     6361 cgtctattac gcgaatgata cgctcgaagt tccgcccttg ctcggttcaa acccgctatg
     6421 ggaaactggc tatcgcgctg accctgaccc tcctgctgct ttcaccactt cagctctttg
     6481 ctcagagcgt tgcgccgcag caaagcgccg gcagcctcaa tttcctcgtg atcagcgact
     6541 ggggcgggaa aggcggaacg gcccaggtag ccgttgccaa acagttgggg ctcaccgctg
     6601 cggctcagaa gagtagcttt gtggttacct gcggcgacaa ctatcacggc agtggcatct
     6661 ccggcgcgga aagccccagg tggaagacgg aatatgagga catttacagc aacccctccc
     6721 tcatgattcc gtggtacccg tcgctgggaa atcacgacaa ccggggcagg gttgaggccg
     6781 agattgccta ttcaaagatc agcccccgct ggaagatgcc ggcgcgttac tacacgcaca
     6841 ccgagaagat cgacggcacg aacgaggcgt tgttcgtgca tctggacacg tccccctttg
     6901 tgacggccta ccgcaaaaag ggctcgtctt atcgcgtagc gggccaggat ccgaaggtgc
     6961 aactgcgctg gcttgactca gtgcttgggg catcacgggc gcgctggaag atcgtcatag
     7021 gacatcatcc ggtttattct gccgcgccaa cccacggcga caccaaggaa ctgattgctg
     7081 acgttctgcc gctgctgcag aaatacggcg tgcaggttta cttctgcggg catgatcatg
     7141 tgctgcaaca tctcagccat ggcggagtaa acttctttgt ttgcggaggc ggctcctcgc
     7201 atcgcacggt gaaccagcgc aacgacgtgc gtttcggcgc cggttccaca ggcttcctgt
     7261 ccatgacgct gactgccgcc gcggcgcaag cgacctatat caatgacaag ggagtagagc
     7321 tttaccggac gagtattccg gcgccctgac ccggttggtc caagaccggc cgctcaatac
     7381 tcgtagaagt cacgagaaag gagctcgcgc tctggcaatt gttgagttgc atcagggtgt
     7441 gggcgcgggc gtctgcggtg ctcccaagtg tcgtttcagg caggcgagca cttcatgcag
     7501 gtccaccggc ttggccagaa agggcagccc cccgaccaga ccgcggcgcg cgcggacttc
     7561 ttccctggta actgcggcag tcaggaacac aatcggcacg cctttcaatt tgggactaat
     7621 ctgtagctgg ctggccaggg tcccgccgtc gaggctcggc atcatcacgt ccagcaggat
     7681 caaggcgggt tggaattcct ccgcggtagc gagcgctgct ttggagacgt tctcggtcgc
     7741 cacctcataa tctccggtct gttccaggtt gagttttagc aaccgcgtaa tactgggttc
     7801 gtcgtccact accaggatgc gtttcttttc gttcatctct ctgtcctcat gcttagccga
     7861 agcgatcggc gcgggtaatc ccgcggaccc tgctccggcg gattgttgtc tcgtcatagc
     7921 tttgcttccg gctcggctct tagcactaca gtcaccacca ctcccccgag cggcgcgttc
     7981 tggatgtcta tcgcgccgcc gtgaaggtcg atgattttct ttacgactga gagacccagc
     8041 ccggtgccga cgcccgtcgg cttggtcgta aagaagggat cgaaaatctt cggtaaatgg
     8101 tcttcggcta tgcccttccc ggtgtcctgc acctcggcga tgaccaccag ctcgccgggc
     8161 ttgaattgga ggaaagccgg tccgctcagc ttgatgtcct cgccgaaacg gctttggcgg
     8221 gtggtgaccg tgagaacgcc gccttcggac atggcctgga gggcgttgag gaagaggttg
     8281 atgaagacct gttccatttt gccgcgatcc agcatcacgc gcggcaaatc ggcgcccagc
     8341 ttggggacca tactgatatg cgcgccaatg atctggctgt taatcagcca cagcgagcgc
     8401 tccaccaagt cgttcaaatc cgcctctttc ggctcgaaat ccttggcggc ggacaattgc
     8461 agcagctcgc ggacgatcac attggcccgg gtgacagcgt cccgcatgtc gctcaggacc
     8521 atcgtcgtgg tgccgttcgt gccgggaagg ttgttggcca gatagtccag tcccatcaga
     8581 atggtctgca agggattctt gacttcgtgg gcgaccccag ccgccagtgt cccgacggat
     8641 tccagtttcg cggcctgaat caactgcagt tgagtcgtct gcaggtcttc attggcagtt
     8701 ttcaacttct gcaaagccgt cttcaaggcg gcttcgtttt gggacaactc cgcataagcc
     8761 cgttccaatc gctcttccga ctgcttgcgc tcggtcaggt ccacgtgtga gccggccatc
     8821 cggaccggct tgccccgggc gtcgcgcagc gccacaccgc gggccagaat ccagcggtag
     8881 ctgccgtcct tatcacggag ccggtgctcc agttcgtaag tcggtgtctg gcccgagaga
     8941 taggcttgca ggcgagcgtg ggcccgctcc cggtcgtcag ggtgcagcaa ttgttcccac
     9001 gcggagaagc ggttctgcac ttcgtggtcg tcgtagccga gcatgccctt ccagcgggtc
     9061 gagaaataga cttcattcgt cgtgagatcc caatcccaca cgccgtcgct gctgccctga
     9121 atcaccagcg ccagtcgttc ctcgctctgc cgcagcgcca gctcggcccg cttgcgctcg
     9181 gtgatgtcgg agaccaggcc gtcataggcg atcagccggc tcagcgtgtt cttgtggggc
     9241 acaggcgtgt tcctgatcca acggatactg ccgttcttgt ggatgatgcg gtgctccagc
     9301 ggcggaggcg tttctccgtt aagaattcgg tcagcctggg ccgtcacggt cggccggtct
     9361 tcgctgtaga tcatgcgata ccagagaaac gggtcggcgt tgaactcccg ggacgaataa
     9421 ccggtgacgg cctcgcaacc cggaccgtga gcagtcgccg taggccggcc gtggtcaacc
     9481 gtgacggaat agacgtagtc ggtgacagag gcgagcagac gcttgtaccg ttgttcgctc
     9541 tcctgcgcgg cctgctcggc gtggttcttt tccttgatcg agaccgtcgt ttgcatggag
     9601 atcttagagg ggatgatcca ctctgtcgga gccaccgaac cgccaggagg agacatccct
     9661 ccccgagggc aaccaaaacc atttagtttc cgcagaactg ttcatcattc agcttttcaa
     9721 tatccgtctg ccattccgtg tcacggctgc ccgaaatccg tgctaacaaa gccagcgagt
     9781 tcgagcaagg gtgatggtaa acttctagca tacaatacag ttattgcaag tattagatag
     9841 ccaagtatta gatagccaag acccgcgccg ttctcgcatt tcctaacgcc tgacagtcct
     9901 tcggcgccag ccacactctc cttgagccga actggtagca caagcaagag ccagaggggc
     9961 cgaggccagg aagtcacgga agaaccggct gtaatctcgg ttgttgtcgt tgatcagccg
    10021 accaccgcaa accgcagccg tcctcgggta atgtcaccag gatttgaagc cgaatttgag
    10081 gtgattgtcg ggtgacggac accttggaat cgggcgtggg cattttgtgc gggacttacg
    10141 cccggccttc cggcaccctg gggcgatgat cgcaaatgat tgcatacgag tgcaaggacg
    10201 ccacttgtct cgccgtgagt tggccggact acaagttttg atcgatgagc atccgcagtg
    10261 gagtcgtcat ggggtggcca aagcgctgtg tcagcggtgg gattggcgga cgccgacggg
    10321 ccgactcaag acgtttgcgg cgcgcagtct gctgctcaag ttagcgcagc ggcaggcact
    10381 gcggttgccg ccggtgcgcg cggccatgcg ccgctctccg tggggattgg gccggccgga
    10441 gctgaggcgg gcgtctgcgc cgcttccccc gctgctggag gccagtttga gccgcttgca
    10501 gcccctggag tggcagatcg gggggcacgg ctcgccggag cgggagcgcg ccctgactta
    10561 cctgcggcac tatcattatc tgggctgcaa tcgtccggtg ggcacgcact tggtgtacgt
    10621 ggtgcgggac gctcaaggac gggatttggc cgttcatctg gtgggggccg cggcttggca
    10681 gtgcgcgcca cgcgatcact atatcggctg gagtgcggct gagcgcgccg cgggcttgca
    10741 tcggattggc aatcacagtc gttttctgat tttgccttgg gtgcgcgtgc cgcacttggc
    10801 cagtcatttg ttgggaggat tagcccaaag actctgcgtg gattggccgg tgcagcatgg
    10861 ctggcaactg gaactgttgg aaacctttgt ggaagcgggc cgttttaagg gggtcgctta
    10921 ccgggcggcc aattggcaac ccgtcggact gaccaccgga cgcacgcgcc aggaaaagga
    10981 gcatcggccg caagctccgc gcaagagcgt gtgggtgtat cccttgggcg agcgctttcg
    11041 agaacgtctg gtagcgcggg gcagccacgg aggtgcacgg tgagtcagcc ccaagctcgc
    11101 ctgccgttgg tgttggaagt gtttgcgctg ttgttttcca aacagtggat cgggcagtgg
    11161 cagaaaaagt ggtgcgcagc ggggcggctg accgggagca cttcgggcca cccagggcgc
    11221 cccaatcaga cttccgcttc cccgccgaag ccgaaacagc ctgcgccagg gcggttctac
    11281 gagcgaatct tctccttgcg ggtcaccctg tggtatttga tttttcagcg gttgaacttc
    11341 gatcagaccc aggcggccgt ggtggtcaac ctgcgggagg gaggcgccga tcgtttaggc
    11401 aagcaacgca agcgcaagct ctcgcgccgg gtacgttcga cccagaccag cgcttacaac
    11461 caggcccggc aacggatgcc tttggagtta ctcgccgagg ccttagcgca cctgcggcaa
    11521 ggtcttttga aactggtggg cttggtgccg gcaccccgcc agcggccggg gcctgccgag
    11581 cgcacgcgcc aaatactgga cggcagcacg ttggccgtgt tggtgacccc actgctggcg
    11641 agggactatc cgcccgcgcg caatcaaagt ggcaccagtg actggtcgct gatgcggatt
    11701 gtcgtgggct tttgcgcgcg gagcggcgcg gtactgagcg ccctggaagg ggcgatgcaa
    11761 cggagcgaac aggcgatggc ctggacgctg atcgaagcgg cggccgcgtt caccctttgg
    11821 gtgggggatc ggaactttgg ggtctggagt gtggcggccc aagccgtacg ctgccatcag
    11881 gatacgctga cgcgcctgac ccgagcccgg gcggccaagc tgacccgggg ccaacccatg
    11941 tacagcggcg aggatcggct catccagtgg tcgcccagcc gtaaagataa ggtcccgccc
    12001 ggcacgaagc gctcggacgt aagcggacgc
//
LOCUS       DATCGS010000364         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_675268, whole genome shotgun sequence.
ACCESSION   DATCGS010000364 DATCGS010000000
VERSION     DATCGS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675268"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIL39_07500"
     CDS             complement(<1..224)
                     /locus_tag="VIL39_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481307.1"
                     /translation="MIRQAYPNHSPAALLPARILTPPLERRIHPADRRAALGLPDESG
                     VPIGMAARIGGGVRMRPVFGIQGAPAGLHQA"
     gene            713..1594
                     /locus_tag="VIL39_07505"
     CDS             713..1594
                     /locus_tag="VIL39_07505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY5481308.1"
                     /translation="MKSMTGYGRGDCSQDGFKIAVELSSVNRKQAEVSVNLPREMEML
                     EAQIRDLVNHCIARGRLTVRVSLHTGVSKSAARMHLNVPLAKAYARELNNLAKQLKLP
                     DPVTLDQLARAPGVLQTDEELAVEEDFWPAVQKALKKALSEMVKMREREGAHLAQDLA
                     TRMTIMRQAAARIQKQAPTVAERYRQQLIERIKSADLEAPGADDERLLREVVYFADRS
                     DISEELTRLQSHFQQFDDCRKSKEPVGRTLDFLAQEINREINTIGSKANDSLISREVV
                     TLKAELERFREQAQNVE"
     gene            1594..2259
                     /gene="gmk"
                     /locus_tag="VIL39_07510"
     CDS             1594..2259
                     /gene="gmk"
                     /locus_tag="VIL39_07510"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5481309.1"
                     /translation="MNTEVAEPVRESPGKAVFVPSPLLVLISAPSGGGKTTLCQQLLA
                     ARSQMTRAVTCTTRPPRAGERDGLDYYFLDAGSFLKRVQAGNFLEHATVYGNSYGILK
                     AEVLGKLRQGKDVLLNVDVQGAATIREKAQEDAELKRALMSVFLTPPSMEIVERRLRT
                     RGTDSEAVIQKRLSVARQEIAQWKNFDYLLLSSSIEEDLRRMLAIVEAEKMRSARVQA
                     PEF"
     gene            2345..2914
                     /locus_tag="VIL39_07515"
     CDS             2345..2914
                     /locus_tag="VIL39_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY5481310.1"
                     /translation="MSIGKNIVLGVTGSIAAYKAAELTSLLTKQGCDVRVVMTADALR
                     FITEVTFKTLSRHPVVTDLYDEAEGWQPEHIKLADEADLLLIAPATANTIAKLALGLA
                     NDALSCVALALNAQARILVAPAMNGKMWLHPATQQNVATLKARGAEFIGPEKGLLSCG
                     YEGLGRLWPVENVSKRALELLRQPRIAKR"
     gene            2901..>3617
                     /locus_tag="VIL39_07520"
     CDS             2901..>3617
                     /locus_tag="VIL39_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481311.1"
                     /translation="MQNDEADLADTLSAAAGGKSAGPATQPRPAVVLFMNVGLWQRLR
                     KYSFEDLAKGLCRLPGRAVELLKIPAPDAVKLLDRIATMERDLILPLKVAGIAMLLYS
                     FYFKLSWIGQRGLGSLEITVQAMQFFLWCYIAVNALVAGLLLAMRRVPLPLVQWAVFA
                     MSLVDGIFLSALVVVTGGYDSILYWLFLGLIVRGAVSVPRATSQILLNLTLTVCYVMA
                     GAINISINKTLEQEREAQAAW"
BASE COUNT          736 a          988 c         1221 g          672 t
ORIGIN      
        1 gcttgatgca gccctgccgg cgccccttga atgccaaaga ccggacgcat cctgacacca
       61 ccaccaatgc gggcggccat gccaatcgga acgccggatt catccggcag ccccagtgcg
      121 gctcgccggt ctgccggatg aatccggcgt tccagtggtg gtgtcaggat gcgcgccggc
      181 agcaaagccg caggagagtg atttggataa gcttgcctga tcaaaaatgg ggttttgagg
      241 gggtctatgg gtataggaca tgtccagggt aggtccagga tgcctccaga tcaccccgag
      301 ggaggcgggc gaggggagtt gtattcgaaa ggcttatcgt gaacaccgaa catccaacat
      361 ccaacttcca acttccaaca tcgaaaggcc gggcacagcc atccaaagtc gggggtgatt
      421 cgtgattcgt gattcgtgat tgggcgggca agccacccca aggctacctg cgcgccaggg
      481 gaacggggca gtaatgcagg gggaatcgga ggaagggcat aatccgatct ttcgcacctc
      541 cgggcaccga cgtgattagt caccgggcgc cgacgttggg aaaggtcaca tcggccgcca
      601 tgccggcgcg gagtttgcct tcgcggttgt cgagccgcac cttgatgccg aagacctgtt
      661 taagatagaa tttgttggcc tgaggtctgc ggattaagca tattctgcct ggatgaaatc
      721 aatgacgggt tatgggcgcg gggattgctc tcaggacggg ttcaagatcg ccgtcgagct
      781 cagttcagta aaccgcaagc aggcggaagt ctccgtgaac ttgccgcgtg agatggagat
      841 gctcgaagcg cagatccgcg acctggtcaa ccactgcatt gcgcggggcc ggttgaccgt
      901 gcgggtgtcg ctgcatacgg gcgtcagcaa atcagcggcg cggatgcatt tgaacgttcc
      961 gctggccaag gcgtatgcgc gcgagttgaa taacctggcc aagcaactca agctgcctga
     1021 cccggtaacg ctggatcaac tggcgcgggc ccccggagtt cttcaaaccg atgaggagtt
     1081 ggcggtggag gaagatttct ggccggcggt gcagaaggcg ctgaagaagg ctttgtccga
     1141 gatggtgaag atgcgggagc gggaaggagc gcacctggcg caagacctcg cgacgcggat
     1201 gaccataatg aggcaagcgg cagcccgcat ccagaagcag gcgccgaccg tcgccgagcg
     1261 gtatcgccag caactcatcg aacgcatcaa gagcgccgac ctggaagcgc ccggagcgga
     1321 cgatgaacgg ttgctgaggg aagtggtcta tttcgccgac cgctcggata tttcggagga
     1381 actcacgcgg ctgcagagtc atttccagca gttcgacgat tgccggaaat ccaaagagcc
     1441 cgtggggcgg acactggatt tcctggccca ggaaatcaac cgggagatca acacgattgg
     1501 atcgaaggcc aatgacagcc tgatttcgcg cgaagtagtg acgcttaaag cggagttgga
     1561 aaggttccgc gaacaggcgc agaatgtaga gtgatgaata ccgaagtcgc cgaaccggtc
     1621 cgggagagcc caggcaaagc ggtctttgtc cccagccctt tgctggtgct gatttcagcc
     1681 ccctccggcg ggggcaaaac cacgctgtgc cagcaattgc tcgcggcgcg gtcgcagatg
     1741 acgcgcgcgg tgacctgcac cacgcgcccg ccgcgcgcag gggagcgcga cggattggat
     1801 tactatttcc tggacgccgg ctctttcctc aagcgcgtgc aggcgggcaa cttcctcgaa
     1861 cacgccaccg tgtacggcaa cagctacggc atcctgaagg cggaggtgct gggcaaactg
     1921 cgtcaaggca aggacgtgtt gctgaacgtg gatgtgcagg gcgcggcgac catccgtgaa
     1981 aaggcgcagg aagacgcgga gctgaagcgg gcattgatgt cggtgtttct gacgccgccc
     2041 tcgatggaga ttgtggaaag acgcctgcgc acgcgcggca cggattcgga ggcggttatt
     2101 caaaagcggc tgagcgtcgc gcggcaggag attgcccagt ggaagaattt tgattacttg
     2161 cttctcagca gcagcatcga ggaagacctg cggcggatgc tggccattgt ggaagcagag
     2221 aagatgcggt cggcgcgggt gcaggcgccg gagttctaac cagaatggtg gagtgcgaag
     2281 cgtgaaacgt gaatcgtgaa ctgacaccgc ggggtggttc acctgtctcg gatcggggtg
     2341 cgaaatgagc ataggaaaga acatcgtatt gggcgtgacc ggctcgattg cggcctacaa
     2401 ggccgccgag ttgacgagcc tgttgaccaa gcagggttgc gacgtgcggg tcgtgatgac
     2461 ggctgatgcg ctgcggttca taaccgaagt gaccttcaag actttgtcgc gtcacccggt
     2521 ggtaacggat ttgtacgacg aagcggaggg ctggcagccg gagcacatta agctggcgga
     2581 tgaagcggat ttgctgctca tcgccccggc gaccgcgaac accattgcca agctggcact
     2641 gggcctggcc aacgacgctt tgagctgcgt ggcgctggca ctgaacgctc aggcccgaat
     2701 cctggtcgcc cccgcgatga acgggaagat gtggctgcat ccagctacgc agcaaaacgt
     2761 ggccacgctc aaggcgcgcg gcgcggaatt catcggcccg gagaaaggcc tgctgtcctg
     2821 cggttacgag gggctgggcc ggttatggcc ggtagagaac gtctccaaac gcgcgctgga
     2881 gctgttgcgg cagccccgga ttgcaaaacg atgaggctga cctggccgat acgctctcgg
     2941 cagcggctgg agggaagtcg gcagggccgg cgacgcagcc gcgtcctgcg gtggtgttgt
     3001 ttatgaatgt cggtctgtgg caacggctta gaaaatactc cttcgaggac ctggcgaagg
     3061 gtctctgtcg gctcccgggc cgggcagtcg agctgttgaa gatcccggca cccgatgcgg
     3121 tgaagctgct ggaccgcatc gccaccatgg aacgggacct catcctgccg ctcaaggtgg
     3181 ccgggattgc catgctcctc tactcgtttt acttcaaact gtcctggatt gggcagaggg
     3241 gattggggag cctggagatc actgttcagg ccatgcagtt ttttctgtgg tgctacatcg
     3301 cggtcaacgc gctggtggcc ggcctgctgc tggccatgcg ccgggtgcct ttgccgctgg
     3361 tccaatgggc ggtcttcgcc atgagcctgg tggatggcat cttcctgtcg gcgctggtcg
     3421 tggtgaccgg cggctacgac agcattctct actggctctt cctgggtttg atcgtgcgcg
     3481 gggcggtgag tgttccgcgg gccacgtcgc aaatcctgct caatctgacg ctgaccgtct
     3541 gttacgtgat ggcgggggcc attaacatct ccatcaacaa aaccctcgag caagagcgcg
     3601 aggcccaggc cgcctgg
//
LOCUS       DATCGS010000365         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_675436, whole genome shotgun sequence.
ACCESSION   DATCGS010000365 DATCGS010000000
VERSION     DATCGS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675436"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..672
                     /locus_tag="VIL39_07525"
     CDS             106..672
                     /locus_tag="VIL39_07525"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY5481312.1"
                     /translation="MFKSLGAKTLIYPAPVLVVGTYDKAGKPNVMTAAWGGICCSQPP
                     CVAVSLRKVTHSHANILARRAFTISIPSEQYVKEADYFGLVSGRNTEKFKAAKLTPVK
                     SKLVDAPYVEEFPLVLECKLVHVAELGLHTQFIGEVIDAKADESIIGDGGAIDVKKLK
                     PLVFAPDTQEYYGIGKFVGKVFSAGKGI"
     gene            673..2859
                     /locus_tag="VIL39_07530"
     CDS             673..2859
                     /locus_tag="VIL39_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEY5481313.1"
                     /translation="MQPPQPASPESLSGLIERVTFFNEENGFAVLKVTVKGHRDEVTV
                     VGSLPSVNPGEWVTAEGRWVRDRDFGLQFRAELLSSAAPTTKEGIQKYLGGGMVKGIG
                     PVYAKKLVHKFGEKIFDIIENYSVRLEEVEGIGPERRRRIKGAWAEQRVIRDIMVFLH
                     SQGVSTSRAVRIYKTYGENAIERVQADPYTLARDIHGIGFITADRIAQKIGIPHDSLL
                     RACAGLDHVLLEATGDGHCALPLELLKEEAGKLLGVEEKIVTAALERTLGSQQLVLEN
                     IGGQELAFLPALKRAEENIALRIRQLCASPAGYPVIELEKAVAWCQQKTGKELAPSQR
                     EALKQALTSRVLIITGGPGVGKTTLVNAILLILCAKKVRCQLCAPTGRAAKRLSEATG
                     LEARTIHRLLEVQHAGGGFGRNESRPLDCDLLVVDETSMVDVPLMSHLLRAMPAQGSL
                     LLVGDVDQLPSVGPGLVLRDLIESGVVPVARLTEVFRQAADSRIITSAHRINQGLLPE
                     LPAKGAEQDFYFVEREEPERIADTLVNLVKRRIPNKFKLDPIRDIQVLGPMNRGSLGI
                     RELNARLQAELNPVRADEPSVEKFGWQFRVRDKVIQTENDYDKEVFNGDIGQIVKIDA
                     VEREVTVSFAPREVVYEFGELDELALAYAITIHKSQGSEFPAVVIPLAMQHYLLLQRN
                     LVYTGVTRGKRLVVLVGQKKALATAVRNNRTERRFSGLLARLRAGE"
BASE COUNT          593 a          865 c         1073 g          570 t
ORIGIN      
        1 gacgaggccg tccccgcccc tttcatgaat tggccctgct tccccggctg gcccgctttt
       61 ctgggcttgg aaccattcgg tggacccggt aacatcccgc tcgttatgtt taaatcactt
      121 ggagctaaga cactgatcta tccggcaccg gtgctggtgg tgggcaccta cgacaaagca
      181 ggcaagccga acgtgatgac cgctgcctgg ggcggcatct gctgttccca gccaccgtgt
      241 gtggcggttt ccttgcgcaa agtgacgcat tctcatgcca atatcctggc gcggcgggct
      301 ttcaccatca gcatcccatc ggagcagtat gtcaaggagg cggactactt cgggttggtg
      361 tccggccgga acacggagaa gtttaaggcg gcaaagctga cgccggtaaa gagcaaactc
      421 gtggacgcgc cttatgtgga ggagtttccg ctcgtgctgg aatgcaaact ggtgcacgtg
      481 gccgagctgg ggctgcatac gcagttcatc ggcgaggtga ttgacgccaa ggccgacgag
      541 agcatcatcg gcgacggcgg ggcaattgat gtcaagaagc tcaagccgct ggtgtttgcg
      601 ccggacacgc aggaatacta cggcattggc aagttcgtgg gcaaggtgtt ctcggccggc
      661 aaaggcatct gattgcaacc gccgcagccc gcgtcacctg agagtctgtc cggcctgatt
      721 gagcgggtca ccttcttcaa tgaggagaac ggctttgcgg tgctcaaggt cacggtcaag
      781 gggcatcgcg acgaagtcac ggtggtcggt tcgctgccat cggttaaccc gggcgaatgg
      841 gtgacggcgg agggccggtg ggtccgcgac cgggattttg gcctccagtt ccgcgcggaa
      901 ctgctgagca gcgcggcgcc gaccacgaag gagggcatcc agaagtattt gggcggcggc
      961 atggtcaagg gcatcgggcc ggtctatgcc aagaagctgg tgcacaagtt cggggagaag
     1021 atcttcgaca tcatcgagaa ctactccgtc cgcctggagg aggtggaggg catcggcccc
     1081 gagcggcggc ggcggatcaa aggcgcctgg gccgagcaga gggtcatccg cgacattatg
     1141 gtgttcctcc attcccaggg agtcagcacc agccgggcgg tgcgcatcta caagacttac
     1201 ggggagaacg ctatcgagcg ggtccaggcc gatccctaca cgctggcgcg agacatccac
     1261 gggatcggat tcatcacggc ggaccggatc gcccaaaaga tcggtatccc gcatgactcg
     1321 ctgctgcgcg cctgcgcggg ccttgatcat gtgctgctgg aggcgaccgg cgacgggcat
     1381 tgcgccttgc ccctggagct gctgaaggag gaagcgggca aactgcttgg ggtggaggag
     1441 aagatcgtca cggcggcttt ggagcgcacg ctgggcagcc agcagttggt cctggaaaac
     1501 atcggcggcc aggaattggc tttcctgcct gcgctcaagc gggcggagga gaacatcgcc
     1561 ctgcgcatca ggcaactgtg cgcctcgccc gccgggtacc ccgtgattga gttggagaag
     1621 gctgtcgcct ggtgccagca gaagaccggg aaggagctgg cgccgagtca gcgggaggcg
     1681 ttgaagcagg cgctgaccag ccgcgtgctt attatcaccg gcggtccggg cgtcggcaaa
     1741 acgaccctgg tcaacgctat cctcctcata ctgtgcgcca agaaggtgcg ctgccagttg
     1801 tgcgctccga ccgggcgcgc ggccaaacgg ctttcggaag cgaccggttt ggaagccagg
     1861 acgatacacc gcctgctcga agtccagcac gccgggggcg gttttgggcg caatgagagc
     1921 cggcctttgg attgtgatct tctggtggtg gatgaaacct cgatggtgga tgtgccgctg
     1981 atgagccatc tgctgcgggc catgcccgcc cagggcagcc tgctgctggt gggcgacgtg
     2041 gatcagttgc cttcggtggg accgggcctg gtgttgcgcg acctgatcga gagcggcgtc
     2101 gtgccggtgg cgcggctgac ggaggtgttt cggcaggcgg ccgacagccg gatcatcacc
     2161 agcgcgcatc ggatcaacca ggggctattg cccgaactgc cggccaaagg cgccgagcag
     2221 gacttctatt tcgtggaacg ggaggagccg gagcggatcg ccgacactct ggtcaatctg
     2281 gtgaagcgcc gcatcccaaa caagttcaag ctcgatccga tccgcgacat ccaggtgctc
     2341 ggcccgatga atcgcggttc gctcggcatc cgcgaactga acgcgcgcct gcaggcggag
     2401 ctgaatcctg tccgggcaga cgagccatcc gtggagaagt tcggctggca gttccgcgtc
     2461 cgcgacaagg ttattcagac cgagaacgac tatgacaagg aggtgttcaa cggggacatc
     2521 gggcagatcg tgaagattga tgcagttgag cgggaggtca ccgtgagttt tgccccgcgc
     2581 gaggtggtgt atgagttcgg ggagttggac gaactggcgc tggcctacgc gatcaccatc
     2641 cacaagtcgc agggctcgga gttcccggcg gtggtcatcc cgctggcgat gcagcattac
     2701 ctgctgttgc agcggaacct ggtctatacg ggggttacgc gcggcaaacg gttggtggtg
     2761 ctggtcggcc agaaaaaggc gctggcgacg gcggtgcgga acaaccggac ggagcggagg
     2821 ttttccgggt tgctggcgag gttgagggcg ggggagtgag ggagcgggcg agtcgcggga
     2881 tgtttgaaaa gagaacaagg actgcggccc tgaaatccga ggaccgaaga ccgaaggccg
     2941 aaagaaggcc gaaacccgaa acccgagagt agtttaatct gttcggccgt cactgcgata
     3001 cgatggctgc ggattcggga cttcgggttt cggccttctt tcggatttcg gccttcggcc
     3061 ttcggatttc ctaactcccc tcatgtccac aaaccccaac t
//
LOCUS       DATCGS010000366         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_376972, whole genome shotgun sequence.
ACCESSION   DATCGS010000366 DATCGS010000000
VERSION     DATCGS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376972"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VIL39_07535"
     CDS             complement(<1..189)
                     /locus_tag="VIL39_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5481314.1"
                     /translation="MAQVVLDRLTKVFQGPGGERVRAVDNLSLVVEDKELLVLVGPSG
                     CGKTTTLRLIAGLEDPTAG"
     gene            634..1857
                     /locus_tag="VIL39_07540"
     CDS             634..1857
                     /locus_tag="VIL39_07540"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY5481315.1"
                     /translation="MNLDALYSELTLKPTAKVALVVMDGVGDIATRETGYLTPLEAAV
                     TPNLDALSKDSAQGRLIPIAPGITPGSGPGHLGLFGYDPLEFQVGRGVIEALGLGLEL
                     RAGDVAARANFCTLDAKGIVTDRRAGRIDTKVCEELCALLAAKVKKVGDAEVIIKPGK
                     GHRFVVIFRGKGLEGPLTDADPHREGFAIPQAAPVDPKSAKAKKAAKLVAEFYKAALP
                     VIAQKKPANGFLMRGIAHQPHIPFFQDRYGMRPACIAVYPMYKGLAQLVGMTKLEGSQ
                     TIAEEFERYLAEYDNYDYFFIHFKYTDMYGEDGNFAAKKQAIEDFDAALPILLRKKPD
                     VLAITGDHSTPCAMKGHSWHPQPVMLQSACSGSDKLERFTETGANSGSLGIIEAKYLI
                     RLMQANAKMFDKFGA"
     gene            1857..2576
                     /locus_tag="VIL39_07545"
     CDS             1857..2576
                     /locus_tag="VIL39_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY5481316.1"
                     /translation="MAKLVFDIETSALPLAQFDEAQQEYLFRECEKIADAAAREVRHA
                     EIQQQFNLWPLTAQVVCIAMLNADTSRGQVLFTAADYDDAADEAGPVEFMACVDEVEL
                     LTAFWDVAKHYETIVTFNGRGFDVPFIYLRSALLNVPISRKDWLGYRFQTDPHCDLME
                     QLTFYSVSGRDGAARRFNLDFYCKAFGIESPKAQGVTGSDVNSLMAEGRYRDIAEYCL
                     RDVKATVLLYHIWRERLAGVK"
     gene            2573..3259
                     /locus_tag="VIL39_07550"
     CDS             2573..3259
                     /locus_tag="VIL39_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481317.1"
                     /translation="MKSEGRKPRTEGNPKGEIRKVLVCFAVKEEARAFQKLAGERGNL
                     KVILVGMGKRNAERAIRAALAEDRPELVLTCGFAGGLRPELAMGMVVFAADPEAGLEP
                     ALLAAGAKPASFHCADRVAATAEEKRGLRESTRADAVEMESQVICAISREQGIPSATV
                     RVILDTANEDLPLDFNQLLTAELKMSYGKLALALAKSPGKVGALLRMQKEAEAAAGKL
                     AEVLGRITAA"
     gene            complement(3246..3680)
                     /locus_tag="VIL39_07555"
     CDS             complement(3246..3680)
                     /locus_tag="VIL39_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5481318.1"
                     /translation="MVKLLSILIIGLIFESAGVVSLKIGITQIGDMKAVSVAEIVKVV
                     KAGVTNVNVLLGVFCEAVFFASLLILMAKSDISFLWPLTGLSFVFATFAAMWFLHERV
                     SSVRWAGVACIMIGAALISYSEHAKPKPSPPSAALESGTTPP"
     gene            complement(3682..>4317)
                     /locus_tag="VIL39_07560"
     CDS             complement(3682..>4317)
                     /locus_tag="VIL39_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEY5481319.1"
                     /translation="LTLLCGHSALPPEQIPGLVNAKGEFTTNPPGAGFRYFFQSSLRE
                     PLRREIHAQFQRFHATGLPLDHVNGHLHLHLHPTVFRILMADAPQLGIKHLRLTFDPL
                     RLNLRLASGHFAYRVLHAAIFHSLSARARPVLAQRGIRHTDAVFGLLQNARVDEDYVN
                     RLLPQLPAGDSELYSHPSLDEFKNEFDALISPRARERVEKLGIKLIRYQDL"
BASE COUNT          912 a         1256 c         1402 g          747 t
ORIGIN      
        1 gcccgcggtg gggtcctcca agccggcaat caagcgcaag gtcgtcgttt tgccgcagcc
       61 tgagggcccg accaggacca gcagttcctt gtcctcaacg acgaggctga ggttgtccac
      121 ggcgcgaacg cgctcgcctc ccgggccctg gaagactttg gtcagccgat cgaggacaac
      181 ctgtgccatg cggaaattgt tggggggcaa cggctgaaag tcgaggccga atcggactgc
      241 cggcaggctg acccgtggga accgggtgtg ccagcccact cctcctcatc cgccctgaag
      301 gggcgagcag tcctgtgcgt gcgtggagcg ggcaaaaacc cttagaacta gaggaacaat
      361 agaggaacaa tagaggaata atagaggaat aatcgagaga ctcacgcggg ctgcccgggg
      421 gtcacaccga cggaacaggc caacctcccg tcctgcgcac cagcgggggt aagacccaac
      481 gcgggagcgt caccggacgc acatgtgaga ctcatgtctg agcccgattc gcgttgacgc
      541 tgctggccgc tttctcgcat catgcaggca gtctgtgacg gcggttggcg ccgtcgcttg
      601 cacccatagg actgaacgaa ctgaaactga cttatgaacc tagatgctct ttattctgaa
      661 ctgactttga agcccaccgc caaggtggca ctggtggtga tggacggcgt gggcgatatt
      721 gccacgcgcg agacaggtta tctcacgccg ctggaagccg cggtcacccc caacctcgac
      781 gcgctgtcga aagactccgc gcagggacgc ctgatcccaa ttgcgccggg catcacgccg
      841 ggcagcgggc cgggacacct gggcctgttt ggctacgacc cgctggaatt ccaggtgggc
      901 cgcggcgtca tcgaggcgct gggattgggc ctcgaactgc gggcgggcga cgtggcggcg
      961 cgcgccaact tctgcacgct cgacgccaaa ggcattgtca ccgaccgccg cgccgggcgc
     1021 attgacacga aggtttgcga ggagctgtgc gcgttgctgg cggccaaggt gaagaaagtc
     1081 ggcgacgccg aggtgatcat caaacccggc aagggccatc gtttcgtggt gatcttccgc
     1141 ggcaaaggtt tggaagggcc gctgacggat gccgacccgc atcgtgaggg gtttgccatt
     1201 ccgcaggccg cgccggtgga ccccaagtcc gccaaggcca agaaagccgc caagctggtg
     1261 gccgagttct acaaggccgc gctgccggtc attgcccaga agaagcccgc caacggtttt
     1321 ctgatgcgcg ggattgccca tcagccccat atcccgtttt tccaggatcg gtatggaatg
     1381 cgccccgcct gcattgcggt ttatccgatg tacaagggcc tggcacagtt ggtgggcatg
     1441 accaagctgg aagggtcgca gaccatcgcc gaggagttcg agcgctacct cgccgaatac
     1501 gacaactacg actacttctt catccacttc aaatacaccg acatgtatgg cgaggacggc
     1561 aatttcgccg cgaagaagca ggccattgag gacttcgacg ccgcgctgcc catcctgctg
     1621 cgcaagaagc ccgacgtgct ggcgatcacc ggcgaccact cgacgccctg cgccatgaag
     1681 ggccattcct ggcatccgca accggtgatg ctgcaatccg cctgctccgg atcggataag
     1741 ctggagcggt ttacggagac cggcgcgaac tcaggctcgc tgggaatcat tgaggcgaag
     1801 tatctcatcc gcctgatgca ggccaacgcc aagatgttcg ataaattcgg cgcttaatgg
     1861 ccaaactggt cttcgacatc gagacttcgg cgctgccact cgcgcagttc gacgaagcgc
     1921 aacaggaata cctgttccgc gagtgcgaaa agatagccga tgcggcggcg cgcgaagttc
     1981 gccatgccga aatccagcag cagttcaatc tctggcccct gaccgcgcag gtcgtctgca
     2041 tcgcgatgct gaatgccgac acctcgcgcg gccaagtgct ctttaccgcg gcggactatg
     2101 atgacgccgc ggatgaggcc ggcccggtgg agttcatggc ctgcgtggat gaggtcgagc
     2161 tgctgacggc gttctgggac gtggcaaagc attacgagac catcgtcacg ttcaacgggc
     2221 gcgggttcga cgtgccgttc atctatctcc gctcagcact gctcaacgtg ccgattagcc
     2281 gcaaggattg gctgggctac cgcttccaaa ccgacccgca ctgcgatctg atggaacagc
     2341 tcacgtttta cagcgtcagc ggccgggatg gcgcggcccg ccggttcaac ctggacttct
     2401 actgcaaggc gttcggcatc gagtcgccca aagcccaggg cgtgaccggg tcggatgtga
     2461 attcgctgat ggccgaaggc cggtatcggg atatcgcgga atactgcctg cgggatgtga
     2521 aggccaccgt gctgctgtat catatctggc gcgagcggct ggcgggcgtg aagtgaaatc
     2581 cgaaggccga aaaccgagaa ccgaaggaaa tccgaagggc gaaatccgaa aggtgttggt
     2641 gtgttttgcg gttaaagagg aggcgcgggc ctttcagaag ctggccgggg agcgcggtaa
     2701 cctcaaagtc atcctcgtgg gcatggggaa gcgcaatgcg gagcgggcga ttcgcgccgc
     2761 gctggcggag gatcggccag agttggtgct aacctgcggg tttgccggcg ggttgcgacc
     2821 cgagctggcg atgggcatgg tggtgttcgc cgccgacccg gaagcgggcc tggagccggc
     2881 actcctcgcg gcgggagcga aaccggcaag ctttcattgc gccgaccgag tggccgcgac
     2941 ggcagaagaa aagcgggggc tgcgcgaatc caccagggca gatgcagtgg agatggaatc
     3001 gcaagtcatc tgcgccataa gccgcgagca aggcattccc agtgcgaccg tgcgagtgat
     3061 tctggacacc gcgaatgagg acctgccact ggacttcaac cagttgctga cggcagagct
     3121 gaagatgagt tacggcaaac tagccctggc cctggcgaaa tcgccgggaa aagtgggcgc
     3181 gttgcttcgc atgcagaagg aagccgaggc agcagccggt aaactcgcgg aggttctggg
     3241 ccgaattacg gcggcgtagt tcccgactca agggcggctg agggaggcga cggtttcggt
     3301 ttggcgtgct cgctgtagct gatcagcgcg gcgccgatca tgatgcacgc cacaccggcc
     3361 cagcggacgg aagaaacccg ctcgtgcagg aaccacatcg ccgcaaacgt cgcgaacacg
     3421 aaactcaagc cggtcagcgg ccagagaaag ctgatgtcgc tcttggccat aagaatcagc
     3481 aggctagcga agaacaccgc ctcgcagaac acacccagca gcacgttcac attggtgacg
     3541 cccgctttga ccacctttac gatttccgcg acgctgactg ctttcatgtc gccgatctgc
     3601 gtgatgccta tcttaagcga aaccacgccg gcagattcga agatcagccc gataatgaga
     3661 attgaaagca gtttgaccat attacagatc ctggtagcga atcagtttga tgccgagttt
     3721 ctcaacccgt tcccgcgcgc gaggactgat taaggcgtcg aattcattct taaactcgtc
     3781 gagtgaggga tgggagtaaa gctccgaatc tcccgccggg agctgcggga gcaggcggtt
     3841 gacgtagtct tcatccacgc gcgcgttctg cagcaggcca aagacggcat ccgtgtgccg
     3901 gatgccgcgc tgggccagaa cgggccgggc gcgcgcggag agcgaatgaa agatggcggc
     3961 gtgaagcacg cgataggcga aatggcccga agccagacgc agattcaacc gcagcggatc
     4021 gaaggtcaga cgcagatgct tgatgcccag ttggggggca tcggccatca ggatgcgaaa
     4081 tacggtcgga tgcagatgga ggtgcagatg gccattgacg tgatcgaggg gcaaaccagt
     4141 ggcgtggaat ctctgaaact gcgcgtggat ctcccgccgt aacggctcgc gcagactgct
     4201 ctggaagaaa tagcggaacc cggcacccgg cgggttagtg gtgaattccc ctttcgcgtt
     4261 caccagcccc ggaatctgtt cgggcggcag agccgaatga ccgcacagca gagtcaa
//
LOCUS       DATCGS010000367         5410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_329030, whole genome shotgun sequence.
ACCESSION   DATCGS010000367 DATCGS010000000
VERSION     DATCGS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329030"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="VIL39_07565"
     CDS             <1..1784
                     /locus_tag="VIL39_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5481320.1"
                     /translation="NQKKIKVTDKKVAILGGGAVAADCATTAKRHGALSVEMIYRRKQ
                     ENMPLTAYERDMLLAQGIEIFSCSKPLAITHKGKRVTGLRIARMMLPKGKSPHPENFV
                     VNQKESPIFREFDVVVSAIGSRSKMPLKKTKGIFYAGDMILGSATVVEAVATGKNAAL
                     EADAFLRGEKAPRFKNRAKNRAVLAGLNLCPVPLEAEFFGRKIRSPFLLSAAPHTDGY
                     AQMRKAYERGWSGGVMKTSFDNVPIHIPSEYMAVVGPSTYGNCDNVSGHPLDRVCQEV
                     GRLIKEFPDRLTLASTGGPVTGHDEADKAVWQSNTRKLERAGAMGVEYSLSCPQGGDG
                     TDGDVVSQNAELAAKIIDWVMEGSSEGNPKLFKLTAAVTSIQPIIKRIQQVFAKYPGK
                     QGGVTLANSFPSLVLRKAENRRWEEGVVIGMSGEGVLPISYLTLAKVSGLGVAVSGNG
                     GPMNYKGAADFLALGAQTVQFCTIAMKYGLGIVDELHAGLSFLMEERGFKSVRELIGS
                     ALPNPITDFMALSATKKLPQVVAELCEHCGNCTRCPYQAITLNGRKIPLFDPALCIGC
                     SLCAQKCFAGAISMRPRTARELAALKH"
     gene            1814..3199
                     /gene="ssnA"
                     /locus_tag="VIL39_07570"
     CDS             1814..3199
                     /gene="ssnA"
                     /locus_tag="VIL39_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HEY5481321.1"
                     /translation="MKPPMSLLIENGTVLTGGKAAAVLPNHSVLIEDGCITQVAPKNC
                     FKRCAARRLDASRKVIAPGFINAHTHFYSTFARGLTKTKPAGNFVDVLKNLWWRLDSA
                     LTTEDCYYSALIALLDSIRHGTTTLIDHHASPQAVRGSLSAIEKAVKQTGLRTCLCYE
                     LSDRDGARISEQGLEENVSFIRHCQRSSQRQSAANDHQLSTIDYQPSTSALFGLHAAF
                     TLKDATLEKAAALGQELGTGFHIHVAEAESDQQYSQQKHHLRVVERLNRFGILGPRSI
                     AAHCVHVNRRELDILSQTQTAVVHNPQSNLNNAVGIADIVAMSKKNVLVGLGTDAMTT
                     NMLEELRVALWAQHLRAKNPSVGFAEVVSALFFNNPRIAERSFNLSLGEVRVGCPGDV
                     VIIDYDPPTPLEASNSFGHMVFGMSQAAVDTTIVGGRVLMENKRLTLDLDEPRINARA
                     RELAAELWKRL"
     gene            3214..4422
                     /locus_tag="VIL39_07575"
     CDS             3214..4422
                     /locus_tag="VIL39_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEY5481322.1"
                     /translation="MNTTYKAIRQRAKALEKDTVRFLADLVRTPSFSSKEEKVIQVIR
                     KEMKKVGFDQVRVDGLGSIIGRIGKGPRVIAFDAHIDTVYPGDREQWSFDPFKPRIKD
                     GKIWGRGTVDQKGGMAAMVYAGKIIKELGLNDQFTIYFTGTVMEEDCDGLCWQYLLDE
                     EKLKPEFVVITEPTNLNIYRGHRGRMEIRVEVKGRSCHGSAPERGDNAIYKIARIALE
                     IEKLNKRLRNDRFLGKGTVTVTEVRSSSPSLCAVADGAGIHLDRRLTAGETKTSALAE
                     VRDAAKRAGCPDAKVFVLNYAEAAYTGKVYPTEKYYPTWVLEEKSPYLKNAVAAYTGV
                     LDKAPLVDKWTFSTNAIAIAGMKGIPCLGLGPGNEIYAHAPNEACPIEHLSRAAAFYA
                     ALVARLNGKV"
     gene            4419..>5410
                     /locus_tag="VIL39_07580"
     CDS             4419..>5410
                     /locus_tag="VIL39_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5481323.1"
                     /translation="MTPFLYQCTTCGKLYQRDEVRYLCPDCAKDYRPGIPLIGVLLAQ
                     FDYVAIRKRFKKLDPNWSLFSAVEDKYYPPYPLGHTPFFPASALGRALGFENVWLKND
                     GLNPSASLKDRASFLVVAEANRLREPSIVTASTGNAASALAAVCAAAGKQAIIFVPES
                     APRAKLVQMVIYGARVIPVRGSYDDAFRLSLEYTARRGGLNRNTGYHPLTIEGKKTVG
                     LEIYEQNRFKAPDVILVPVGDGVIISGVFKAFHDLKAAGLIAKLPRLVCVQAERSAAI
                     HHYIRCGKYQDARRPDTIADSISVSIPSNAHLARKVVLESRGFSITVTDQEILRG"
BASE COUNT         1143 a         1648 c         1604 g         1015 t
ORIGIN      
        1 agaaccagaa gaagatcaag gtcacggata agaaggtagc catccttggc ggcggcgcgg
       61 tggccgccga ctgcgcgacc acagccaaga ggcacggggc gctctcggtc gagatgatct
      121 atcgccgcaa gcaggagaac atgccgctga cagcctacga gcgggatatg ttgctcgcgc
      181 agggcatcga gatcttcagt tgctcgaagc cgctcgcaat cacgcataaa ggcaagcggg
      241 ttaccggcct ccgtatcgcc aggatgatgc tgccaaaggg caaatcgccc catccggaga
      301 actttgtcgt caatcagaag gagtctccca tattccggga gttcgacgtg gttgtctcag
      361 ccattggcag ccggtcgaag atgccactca agaagaccaa gggcatcttt tatgcgggcg
      421 atatgatcct aggctccgcg accgtcgtgg aagcggtagc caccgggaag aacgccgcgc
      481 tggaagccga tgcgttcctg cgcggcgaga aagcgccccg cttcaagaac cgcgccaaga
      541 accgcgccgt tctggcgggt ttgaatctct gcccagtgcc ccttgaggct gagttctttg
      601 gccgcaagat acggtcaccg ttcctcctgt ccgccgcgcc gcacaccgac ggctatgccc
      661 agatgcgcaa ggcctatgag cgcggctggt ccggcggggt gatgaagacc tccttcgaca
      721 acgtgccgat ccacatcccc tccgaataca tggctgtggt tggcccctcg acctacggca
      781 actgcgacaa tgtctcgggc catcccttgg accgggtgtg ccaggaagtc gggcggttga
      841 taaaggagtt tccggaccgg ctgacgctcg cctcgaccgg cgggcccgtc actggccatg
      901 atgaggcgga caaagccgtc tggcaatcga acacccgcaa gctggaacgc gccggagcga
      961 tgggcgttga atacagcctc tcctgtccgc agggcggcga tggcacggac ggcgatgtcg
     1021 tttcccaaaa tgccgaactg gcggccaaga tcattgattg ggtcatggag ggcagttccg
     1081 agggcaaccc gaagctgttc aagctcaccg ccgccgtcac ctccattcaa ccgatcatca
     1141 agaggattca gcaggtcttt gcgaagtacc ccggcaagca gggcggcgtc accctggcaa
     1201 acagcttccc ttcactcgtt ctgcgcaagg cggagaaccg gcgttgggag gaaggcgtcg
     1261 tgattggcat gtccggcgaa ggggtcttgc ccatcagcta tctgacgctg gcaaaggtct
     1321 cgggcttggg cgtcgccgtg tccggcaacg gcgggccgat gaattacaaa ggggccgcag
     1381 actttctggc tttgggcgcc cagaccgtcc agttctgcac catcgcgatg aagtacggac
     1441 tgggcattgt agatgagctt catgccgggc tgagcttcct gatggaagaa cggggattca
     1501 agtcggtgcg ggaactgatt ggctcggcgc tgcccaatcc aattaccgat ttcatggcgc
     1561 tctcggccac caagaagctg ccgcaggtgg tggcggaact ctgtgagcat tgtggtaact
     1621 gcacccgctg cccctaccag gccatcactc tgaacggcag aaagatcccg ctcttcgatc
     1681 ctgcactttg catcggctgt tcgctctgcg cgcagaagtg ttttgccggc gccatctcca
     1741 tgcgcccgcg aactgcccgt gaattggccg ccctgaagca ctagcccggg taacctttgt
     1801 aacccttgta acgatgaaac cgccaatgtc cctgctcatt gaaaacggca cggtgctgac
     1861 cggcgggaaa gcagccgccg tcttacccaa ccactccgtc ctcatcgaag acggctgtat
     1921 tactcaggtc gctccaaaga actgtttcaa gcgatgtgct gccaggcgcc ttgatgcctc
     1981 gcgcaaagtg atagctcccg gcttcatcaa tgcccacacc catttctaca gcaccttcgc
     2041 tcgcggcctc accaagacca agcccgccgg gaactttgtg gacgtgctga agaacctctg
     2101 gtggcggctc gactcggcct taaccaccga ggattgctat tacagcgcgc tgatcgccct
     2161 gctcgattcg atccgtcacg gcacgacgac gctcatagac caccacgcga gcccccaggc
     2221 ggttcgcggt tccctgtcgg ccatcgagaa ggcagtcaaa cagaccggcc tgcggacctg
     2281 tctgtgctat gaactgtccg atcgagacgg cgcgcgtatt tccgagcagg gcttggagga
     2341 gaacgtctcg ttcatccgcc actgccagcg cagcagccaa cgtcagtcgg ctgcaaatga
     2401 ccaccaacta tcaactatcg actatcaacc atccacctcg gccctcttcg gccttcatgc
     2461 ggcatttacg ctcaaggacg ccacgctgga gaaagctgca gccttgggcc aggaactggg
     2521 aacaggcttt catatccacg tcgccgaggc cgagagcgac cagcaatact cccagcagaa
     2581 gcaccacctg cgagtagtgg agaggctgaa ccggttcggc atcctgggcc cccggagcat
     2641 agccgcgcat tgtgtgcatg tgaatcgccg ggagctggat atcctgtctc aaacgcaaac
     2701 cgcggtcgtt cataaccccc agtcgaactt gaacaacgcg gtgggcatcg cggacatcgt
     2761 tgccatgagc aagaagaacg tgctggttgg cctgggcacc gacgccatga cgaccaacat
     2821 gctcgaagaa ctgcgcgtcg cattgtgggc tcaacatctg cgcgccaaaa acccttcggt
     2881 gggtttcgcc gaagtggtgt cagcgctctt cttcaacaac cccagaatcg cagagagaag
     2941 cttcaacctt tcgctgggtg aagtccgtgt ggggtgcccc ggagacgtag taatcataga
     3001 ctatgatcct cccaccccgc tggaggccag caacagtttc ggccacatgg tcttcgggat
     3061 gtcccaggcc gcagtggata cgaccatcgt cggcggaaga gtgctaatgg agaacaagcg
     3121 cctgacgctc gaccttgacg agccgcggat caatgctcgc gcccgcgagc tggcagcaga
     3181 gctatggaag agactttagc ccgaagattc agaatgaaca cgacctacaa agccattcgg
     3241 caaagagcca aagcgctcga aaaggatacc gttcgcttcc tcgcggacct tgtccgcaca
     3301 ccttcctttt cctccaaaga ggagaaggtc atccaagtca tcaggaagga gatgaagaag
     3361 gtcgggttcg accaggttcg cgtggacggt ttgggcagca ttatcgggcg gattggcaag
     3421 ggcccgcgcg tcattgcctt cgacgcccac attgacaccg tctatcccgg cgaccgcgag
     3481 cagtggagct tcgatccctt caagccgaga atcaaagacg gcaagatctg gggccgcggc
     3541 actgtggatc aaaagggcgg catggccgcg atggtctatg cgggcaagat catcaaggag
     3601 ctgggcttga atgaccagtt cacgatctat ttcaccggca cggtcatgga agaggactgc
     3661 gacggcttgt gctggcagta cttgctcgac gaagagaagc tcaagccgga attcgtggtc
     3721 attaccgaac cgacaaacct gaacatctat cgcggtcatc ggggccggat ggaaatccgg
     3781 gtggaagtta aaggccgcag ttgccacggc tccgcgccgg aacggggcga caatgccatc
     3841 tataagatcg cccgcattgc actcgagatt gagaagctga ataagcgctt gcggaatgac
     3901 cggttcctgg ggaaggggac ggtcacggtt accgaggtgc gctcctcgtc gccgtcgctc
     3961 tgcgccgttg ccgacggggc gggtattcac ctggatcgca ggcttaccgc cggcgagacc
     4021 aagaccagcg ccctggccga ggtgcgggac gcggcgaagc gggcaggctg ccctgacgcc
     4081 aaagtattcg tgctcaatta cgccgaggcc gcttacactg gcaaggtgta tccgacggag
     4141 aagtattacc ccacctgggt gctggaggag aagtcgccct atctgaaaaa cgctgtcgct
     4201 gcctataccg gtgtgctgga caaggctccg ctggtggaca agtggacctt cagcaccaac
     4261 gccatcgcca tcgccggcat gaaggggatt ccgtgcctgg ggcttgggcc gggcaacgag
     4321 atctatgcgc acgccccgaa cgaagcctgc cccatcgagc acctcagccg cgccgcagct
     4381 ttctacgcgg cgttggttgc ccggctcaac ggaaaagtat gactccgttc ctctaccagt
     4441 gcaccacttg cggcaagctt tatcagcgcg acgaagtccg ctatctctgc ccggactgcg
     4501 ccaaagatta tcggccaggc attccgctga tcggagtcct gctggcccag ttcgattacg
     4561 tggccatacg gaagcgcttt aagaagctgg atccaaactg gagcttattc tccgccgtcg
     4621 aggacaagta ttatcccccc taccctctcg gccacacgcc tttctttccg gcctcggccc
     4681 taggcagggc gctcggcttc gagaatgttt ggcttaagaa cgacggcctg aatcctagtg
     4741 cttcactgaa ggaccgagcc tcattcctcg tcgtggccga ggccaatcgg ctccgagagc
     4801 cctccatcgt cacagcctcg acgggcaatg ccgccagcgc actggccgcc gtctgcgcgg
     4861 ccgcgggcaa gcaggcgatc atctttgttc ctgaaagcgc cccgagagcc aagctcgtcc
     4921 agatggtgat ctacggtgcg cgcgtcattc cagtgcgagg ctcatacgat gacgcgttcc
     4981 ggctgtccct cgaatacacc gcccgcaggg gcggcctcaa ccgcaatact ggctaccatc
     5041 cgctgacaat agaaggcaag aagacggttg ggctggagat ctacgagcag aaccggttca
     5101 aagcgccaga cgtgatcctc gtgccagtcg gggatggagt gataataagc ggcgtattca
     5161 aggcgtttca tgatctgaag gcggcggggt tgattgccaa attgccacgg ttggtctgtg
     5221 tccaggcgga gcgttccgcg gccatccatc attatattcg gtgcgggaag taccaggatg
     5281 cccggcggcc cgacaccatc gccgactcga tttccgtctc cattccctcc aatgcgcacc
     5341 tggcacgaaa ggtggtgctc gaaagccggg gattctcaat caccgtcacg gaccaggaga
     5401 ttctgcgcgg
//
LOCUS       DATCGS010000368         3430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_377007, whole genome shotgun sequence.
ACCESSION   DATCGS010000368 DATCGS010000000
VERSION     DATCGS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377007"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VIL39_07585"
     CDS             <1..456
                     /locus_tag="VIL39_07585"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY5481324.1"
                     /translation="LESVGMRGGIPGNDSSRQFNQAEAFGNLNLPWGWDLGKSWHLRP
                     RLDLSAGWLGDKGNNTAIGTVGPSLVVNRGQFPVSLEGGISPTILSDNDFASKNFGTD
                     VQFTSHLGLNWGFARHWCLSYRYQHMSNAGLASRNPGLNLHVFGLSYKF"
     gene            460..3219
                     /gene="acnA"
                     /locus_tag="VIL39_07590"
     CDS             460..3219
                     /gene="acnA"
                     /locus_tag="VIL39_07590"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5481325.1"
                     /translation="MSKAQNLFGTLGTFDLGNGRQGALHALPQLEKAGVGAVSRLPVS
                     IRLVLESVLRNCDGQRVLEQNVRALANWKPTGPRTEEIPFVVARIVLQDFTGVPLLVD
                     LAAMRSALGRLGRNPKLIEPLVPVDLVVDHSVQVDYYGTADALGKNLELEFSRNRERY
                     QFLKWGMQAFETFKVVPPGIGIVHQVNLEYLAKGVLCCPSTINHQPSTLFYPDTLVGT
                     DSHTTMINGLGIVGWGVGGIEAEAGILGQPVYFLTPDVVGVHLTGALREGVTATDLAL
                     TVTQMLRKAKVVGKFVEFFGPGAAALPVVDRAMIANMAPEYGATMGLFPIDAECVSYL
                     RATGRSEEHCRIYENYYRAQGLFGMPQKGEIQYSVELELDLATVVPSVAGPKRPQDRI
                     ELPNLKREFAAAFTRPVTENGFGRTAADLGKSFPVSVKGGFQANGGIRHGSVVIAAIT
                     SCTNTSNPSVMLAAGLLAKKAVERGLQVNPLVKASLAPGSRVVTDYLTRTGLQTYLDQ
                     LGFNLVGYGCTTCIGNSGPLPAPVDEAIAKHDVIAASVLSGNRNFEARVHQSIKANFL
                     MSPPLVVAFALAGRVDIDLSREPIGKGKGGEDVYLKDIWPTLQEVRDRMEAALKPEVF
                     RQLYRDFAGQNPKWNEIPASGGNVYAWDASSTYIQEPPFFQEFGLQPGEIQEIKGARA
                     LAIFGDSVTTDHISPAGSIKPSSPAGKYLVEQGVAVADFNSYGSRRGNDRVMTRGTFA
                     NVRIKNLMAPGTEGGVTRCFAGKDCPCAPGEQISIYDAAMAYQKERTPLVIIAGQEYG
                     TGSSRDWAAKGTALLGVKAVVAQSFERIHRSNLLGMGVLPLQFKEGTSASTLKLDGTE
                     TFDVVGLGQGLKPQQDLTLRVTRANGQVETIPVTCRIDTPIEIDYYQHGGILPYVLRQ
                     LVGAN"
     gene            3239..>3430
                     /locus_tag="VIL39_07595"
     CDS             3239..>3430
                     /locus_tag="VIL39_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481326.1"
                     /translation="MEREPLHVVRSRRSWSLSPQVPPGIPALAEKIIQAASREHPADG
                     VMRAELKAQHGLSRDDGARV"
BASE COUNT          655 a          920 c         1230 g          625 t
ORIGIN      
        1 ctggaatctg tagggatgcg tggcggcata cctgggaacg acagcagccg gcagttcaac
       61 caggccgagg cgtttgggaa tctcaatctg ccgtggggtt gggacctggg taagagctgg
      121 catctgcggc cccggctgga tctttcggct ggctggctgg gcgacaaagg caacaacacc
      181 gccatcggga ctgtcgggcc gagcctggtc gtgaaccggg ggcagtttcc ggtgtcgctc
      241 gaaggaggaa ttagccccac gatcctaagc gacaacgatt ttgcctcgaa gaactttggc
      301 acggatgttc agtttacgag ccaccttgga ctgaactggg gttttgcccg gcactggtgt
      361 ctcagctacc gctatcagca catgtccaac gcgggtctgg cctcaaggaa tcccggtttg
      421 aatctgcatg tgtttggcct gagctacaaa ttctgagtta tgagcaaagc acagaacctg
      481 ttcggcacgt tggggacctt cgatctcggg aacggcaggc aaggggcgtt acatgcgctg
      541 ccgcaactgg agaaggcggg cgtgggagcg gtgtcccggc tgccggtgtc gatccggttg
      601 gtgctggagt cggtgctgcg caattgcgac ggccagcggg tgctggaaca aaacgtgcgc
      661 gcgctggcca actggaaacc gacggggccg cgcaccgaag agattccctt tgtggtggcg
      721 cgcatcgtgt tgcaggactt cacgggggtg ccgctgctgg tggacctggc ggcgatgcgg
      781 tcagcgctgg ggcggctggg ccggaatccc aagctgatcg agccgctggt gccggtggac
      841 ttggtggtgg accactcggt gcaggtggac tactacggca cggcggatgc cctgggcaag
      901 aacctggagc tggagtttag tcgcaaccgg gagcgttacc agttcctcaa gtggggcatg
      961 caggctttcg aaaccttcaa ggtggtgccc ccgggcatcg gcatcgtgca ccaggtgaac
     1021 ctcgaatacc tcgccaaggg cgtgttgtgc tgcccatcaa ctatcaacca tcaaccatca
     1081 actctcttct accccgacac cctggtggga acggattctc acaccaccat gatcaacggg
     1141 ctgggcattg tgggctgggg cgtcggcggc attgaggcgg aggcgggcat actgggtcag
     1201 ccggtgtatt tcctcacgcc ggatgtggtg ggggtgcacc tgacgggtgc tttgcgggaa
     1261 ggcgtgacgg cgacggacct ggcgttgacc gtcacacaga tgctgcggaa ggcgaaggtg
     1321 gtggggaagt ttgtggagtt cttcgggccc ggggccgccg ccctgccggt ggtggaccgg
     1381 gcgatgatcg ccaacatggc gcccgaatat ggggcgacga tggggctctt cccgattgat
     1441 gcggagtgcg tgagctattt gcgggcgacg ggccgcagcg aggagcattg ccggatttat
     1501 gagaactact accgggcaca gggattgttt ggcatgccgc agaagggcga gattcaatat
     1561 tcggtcgagc tggaactgga cctggcgacg gtagtgccga gcgtggcggg gccgaagcgg
     1621 ccgcaggaca ggatcgaatt accgaatctg aagcgggagt tcgcggcggc attcacgcgg
     1681 ccggtcacag agaacggctt cggcagaacg gcggcggacc tgggcaagag cttcccggtt
     1741 tccgtcaaag ggggctttca ggcaaacggc ggtatccgcc acgggagcgt ggttattgcg
     1801 gcgatcacca gttgcaccaa cacgagcaat ccgtcggtga tgctggcggc aggcttgctg
     1861 gcgaagaagg ccgtggagcg cggcttgcag gttaatccgc tggtgaaggc gtcgctggcg
     1921 ccgggttcgc gcgtcgtgac cgattatctg acccggaccg gtttgcagac gtatctcgat
     1981 cagttaggct tcaacctggt cggttatggc tgcacaacgt gcatcggcaa ttccgggccg
     2041 ctgccggcgc cggtggacga agcgatcgcc aagcacgatg tgattgcggc gtcggtgctg
     2101 tccggcaacc gcaacttcga ggcgcgggtg catcagagca tcaaggccaa ctttctcatg
     2161 tcgccgccac tggtcgtggc ctttgcgctg gcgggtcgag tggacattga cctgagccgg
     2221 gagccgatcg ggaaggggaa aggcggcgag gatgtttacc tgaaggacat ttggccgacg
     2281 ttacaggagg tgcgcgaccg gatggaggca gcgctcaagc cggaagtgtt ccgccagctc
     2341 tatcgcgact tcgcagggca gaatccgaag tggaacgaaa tccccgcgag cgggggcaac
     2401 gtttacgcat gggacgccag cagcacgtat atccaggagc ctcccttctt ccaggagttc
     2461 gggttgcagc ccggagaaat ccaggaaatc aaaggcgcgc gggcgctggc gatttttggc
     2521 gacagcgtga cgacggatca catctcgcca gcgggcagca tcaagccgag ctcgccggcg
     2581 gggaaatacc tggtggagca gggcgttgcg gtcgcggatt tcaacagcta cggctcgcgc
     2641 cgcggcaacg accgggtgat gacgcgcggg acctttgcga acgtgcggat caagaacttg
     2701 atggccccgg gcaccgaggg cggggtgacc cggtgcttcg cgggcaaaga ttgcccgtgc
     2761 gccccgggcg aacagatttc gatctacgat gcggcgatgg cctatcagaa ggagcggacg
     2821 ccgctggtga taatcgccgg gcaggaatat ggtacgggca gctcgcggga ttgggcggcc
     2881 aaagggacgg cgttgctggg ggtgaaggcg gtggtggcgc agagcttcga gcgtatccat
     2941 cgttcgaacc tcttgggcat gggagtgctg ccgctgcaat tcaaggaggg cacgagtgcg
     3001 agcactttga aactggacgg cacagaaacg ttcgacgtgg tgggcttggg gcaggggctg
     3061 aaaccccagc aggatctgac gctgcgcgtg acgcgcgcga acgggcaggt ggagacgata
     3121 cccgttacgt gccgcattga tacgcccatt gagatcgatt actatcaaca cggcgggatc
     3181 ttgccctacg tgctgcggca gttagtgggc gccaactgaa atacggcctc gaatcaggat
     3241 ggaacgtgaa ccattacatg tcgtgcggtc gcgcagaagc tggagtctga gcccgcaagt
     3301 tccgccggga attccggccc tggcggaaaa gatcatccag gctgccagcc gcgagcatcc
     3361 cgcggacggg gtgatgcggg cggaattgaa ggcgcagcac gggttgtcac gggatgacgg
     3421 cgcacgggtc
//
LOCUS       DATCGS010000369         5168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_226734, whole genome shotgun sequence.
ACCESSION   DATCGS010000369 DATCGS010000000
VERSION     DATCGS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226734"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1681
                     /locus_tag="VIL39_07600"
     CDS             248..1681
                     /locus_tag="VIL39_07600"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5481327.1"
                     /translation="MSQPLIIQGGMGVAVSGWPLARAVSLAGQLGVVSGTALGVVLTR
                     RLQLGDPGGDLRRALARFPFPRMADRLLADHFIPGGKSPLTPFKLTPMPTLQSRPALV
                     ELTVIANFVEVFLAKEGHTGPVGINYLEKIQLPTVPSIFGAMLAGVDYVLMGAGIPRA
                     IPGVLDHLARGETAQLPVNVEGSLPGEKHLLTFSPTSFCGGAPPQLKRPQFLGIVASA
                     TLAMTLARKSSGRVDGFIVEGPTAGGHNAPPRGPLQLDSAGEPVYGPRDVPELEKIRA
                     LGLPFWLAGSYGRPGKLAEALRLGAAGVQVGTAFAFCEESGVQPELKREALRLSRLGQ
                     ARVFTDPLVSPAGFPFKVAQMAQTLSEVALYENRPRICDLGYLRHPYRKTDGTVGYRC
                     PAEPLKNFLQKGGILADIQGRKCVCNGLPATVGLGQVRPKSGAELPLVTAGDDVADIA
                     QFLKPGRDSYSAQDVLDRLLAEPAVNS"
     gene            complement(1696..1893)
                     /gene="rpsU"
                     /locus_tag="VIL39_07605"
     CDS             complement(1696..1893)
                     /gene="rpsU"
                     /locus_tag="VIL39_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY5481328.1"
                     /translation="MTEIRLKKGEPVERALRRLKKKLDREQTLQQFRLRRRYEKPSAK
                     KRRKEKAARFEAMLKARHADD"
     gene            complement(2318..2605)
                     /locus_tag="VIL39_07610"
     CDS             complement(2318..2605)
                     /locus_tag="VIL39_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY5481329.1"
                     /translation="MAKALTKSQIVASVAETAKLTKKQAGEILEIIVALAYKNAKNSF
                     TIPGLGKLVLVNRKARMGRNPATGAAIKIPAKRVVKFRVAKAAKDAILGAK"
     gene            2843..4204
                     /gene="asnS"
                     /locus_tag="VIL39_07615"
     CDS             2843..4204
                     /gene="asnS"
                     /locus_tag="VIL39_07615"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY5481330.1"
                     /translation="MRTLVKHVLAATEPKEAVTVKGWVRTRRDSKGFSFLELNDGSCL
                     ANVQVVVDAGTPGSEHITQLTTGASVVVEGKLVASPAQGQKWELRATRVELVGPADAT
                     YPLQKKGHTQEFLRTIAHLRPRSNLFGAIFRLRSRLAFAVHQFFQERDFVYVHTPIIT
                     ASDCEGAGEMFRVTVLKGNIDEKPQTDFFGKRAYLTVSGQLEGETFACALSNIYTFGP
                     TFRAENSNTARHAAEFWMIEPEMAFCDLQGDMDLAEEFVKAMARCALEHCAEDLALFA
                     KFVDKGLHERLKFVVERPFVRVPYSEAVEILKKSGKKFEFPVEYGLNLQSEHERFLTE
                     EHFKSPATVFNYPREIKPFYMRLNDDGKTVTAMDVLVPGIGEVIGGAQREERPEQLLE
                     NMKSHKLKPEDYWWYADLRRYGTVPHAGFGLGFERLLMFLTSVPNIRDVIPFARTPGN
                     ADF"
     gene            complement(4639..4869)
                     /locus_tag="VIL39_07620"
     CDS             complement(4639..4869)
                     /locus_tag="VIL39_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481331.1"
                     /translation="MRSAAAEHFRSLNQEVQSRLNRSFDAEDAKMSALHARWVHEALS
                     SGNSTALKPAELDQAFARGIARAKRRKTAKAA"
BASE COUNT          946 a         1627 c         1559 g         1036 t
ORIGIN      
        1 gctatgttgc taacccaaaa gaccctggct ggcgagtttc aaatagggga tcagtgacgg
       61 cgccgcagac ggccgcagac ggtgcttgcg ttgtcgtatg cttcaccgta ggcttattgc
      121 acgcgtcggt ttccgacggg ctcctgacct tggtgcgcca atggacggac tgtgcgaacg
      181 actccaggct ttccggtcgc gtcattcccc tcgagtcgat ggggataggt cgtggatctg
      241 tttaactatg tcacaaccct tgatcatcca gggcgggatg ggcgtggcgg tgtccggctg
      301 gccgctggcc cgggccgtct cgcttgcggg ccagctcggt gtcgtttccg gaacggcgct
      361 gggcgttgtt ctgacgcgcc gcctccagct tggcgacccc ggcggcgatc ttcgtcgcgc
      421 gttggctcgc ttcccttttc cacgcatggc cgaccgcctg ctcgcggacc attttatccc
      481 cggcggcaag tcccccctga cgcccttcaa gctcacgccc atgcccacgc ttcagtcccg
      541 gcccgcgctg gtggaactga ccgtcatcgc caacttcgtg gaagtcttcc tggccaagga
      601 aggccacact ggaccggtcg gcatcaacta tctcgaaaag atccaactgc ccacagtgcc
      661 atcaatcttc ggtgccatgt tggccggcgt tgattacgtg ctcatgggtg cggggattcc
      721 acgcgccatt cccggcgtgc tcgatcatct ggcccgaggc gagacggccc aattgcccgt
      781 caacgtcgaa ggatcactcc ccggcgaaaa gcatctcctc accttcagcc cgaccagctt
      841 ctgcggcggc gccccgcctc agctcaagcg gcctcaattc ctgggcattg tcgcttctgc
      901 caccttggct atgactctgg ccaggaaatc cagcggccgc gtggatggct tcatcgtcga
      961 aggccccact gcgggagggc acaacgctcc gccgcgcggc ccgctccaac tcgattccgc
     1021 cggcgagccg gtctatggcc cgcgcgacgt gcctgagctc gaaaagatcc gtgctttggg
     1081 gttgccgttt tggctggccg gctcctatgg ccgccccggc aaacttgccg aggccctgcg
     1141 tctgggtgcc gctggcgtgc aggtggggac ggcgtttgcc ttctgcgaag agtccggcgt
     1201 ccagcctgag cttaaacggg aagccctccg cctgagccgc ctcggccagg ctcgcgtctt
     1261 taccgacccg ctggtttcac ccgcgggatt tcccttcaag gtggctcaaa tggcgcaaac
     1321 cctctctgag gttgcgctct atgaaaaccg cccccggata tgcgaccttg gctacctgcg
     1381 ccacccctac cgcaagaccg atggcacggt cggctaccgc tgtcccgccg agccgctcaa
     1441 gaatttcctg caaaaaggcg gcatcctggc agacatacag ggccgtaaat gcgtctgcaa
     1501 cggcctcccc gccaccgtgg gcctgggtca ggttcggccc aaatccggag ccgagctgcc
     1561 gctggtcact gccggtgacg acgtggcgga catcgcccag ttcctgaagc ccggccgcga
     1621 ctcctactcc gcccaagacg tgctcgatcg gctcctggcc gaaccggcag tcaattcctg
     1681 acgcacctcg gcaggtcagt catcggcatg ccgcgccttt agcatcgcct cgaaccgggc
     1741 ggccttttcc ttccggcgct tcttggcgct gggcttttca tagcgccggc gcagccggaa
     1801 ctgctgtaac gtttgttcgc ggtccagctt cttcttcagc cggcgaaggg ccctctcgac
     1861 cggttcgccc tttttcaatc taatttcagt catatatacg ttcttaggca gagccgctgc
     1921 gcttcgcgca gaccgtcccc gccggcggct ttccgccatc cggcacaact ctgaactccc
     1981 aactctcaac tcgcaactgc tttccatacc attaccgtgt ccagttgcac aatcagcaat
     2041 accgccaccc accgtaccga cccgtgaggt tcttcgattt caacgcaaag atcaacacca
     2101 acgtcaacac caacgtcaac agactgatga gcactcggaa cctccgtcga gccggtgaac
     2161 gccactttgc ggcaaagcga gttgctgaaa aactccttac cgaatctatt ggacgagccg
     2221 cacaccactt gcaggactcc cttgctcaac tgcgctgcct gcaaaaaaag gcggaggaaa
     2281 ttcctccgcc ttcacaagga taatgatttg gttcgcctta cttggcgccg aggattgcgt
     2341 ccttggctgc cttggccacg cggaatttca ccacgcgctt ggcaggaatc ttgatcgccg
     2401 ccccggtggc cggattgcgg cccatgcggg ctttccggtt tacgagcacg agcttgccca
     2461 acccaggaat cgtgaagctg ttcttcgcgt tcttgtaagc gagcgccacg atgatttcga
     2521 gaatctcgcc ggcctgcttc tttgtgagct tagcggtttc cgcaacagat gcgacaattt
     2581 gtgatttggt taatgctttt gccatatatg agtgccatag ctagcggcaa agcgagattg
     2641 gcgcaagcta aaaacagcaa aaaaactcac ccgccggcct gctccaaagc ctggccggtc
     2701 gaagggagaa ccaggcccgc aaggctgaaa tccgagaatg gcctgatccg atgggctgct
     2761 ccttctgatc gagccttgtg ccttgcggcg cggactcttt cagctaggct tgcggcaaga
     2821 ttgaagcaac cgaattattc gtatgcgcac actggttaag cacgtcctcg cagccaccga
     2881 accgaaggaa gcggtaaccg tcaaaggctg ggtccgcact cggcgcgact cgaagggttt
     2941 ctccttcctc gaactgaacg acggctcctg tctcgcgaat gtgcaggtgg tcgtggatgc
     3001 cggcacgccc ggctcggaac acatcaccca gctcacgacg ggggcgtcgg tggtggtcga
     3061 ggggaagttg gtggcgtccc cggcgcaggg ccagaaatgg gaactgcggg ccacgcgcgt
     3121 cgagctggtc gggccggcgg atgcgacgta tccgttgcag aagaagggcc acacgcagga
     3181 gttcttgcgc acgattgcgc atctgcgtcc gcgctcgaac ctgttcgggg ccatcttccg
     3241 cctgcgcagc cggctggcgt ttgcggtgca tcaattcttt caggagcgcg actttgtcta
     3301 tgtgcacacg ccgatcatca cggcgagcga ctgcgagggc gcgggcgaga tgttccgcgt
     3361 caccgtcctc aagggaaaca ttgatgagaa gccgcagacg gatttcttcg gcaaacgggc
     3421 ctacctcacg gtcagcggcc agctcgaagg agagacgttc gcctgcgcgc tgtccaacat
     3481 ctacaccttc ggcccgacct tccgcgccga gaactcgaac acggcgcgcc acgctgcgga
     3541 gttctggatg atcgagccgg agatggcctt ctgtgatttg cagggggaca tggacctggc
     3601 cgaagagttc gtgaaggcca tggcgcggtg tgcgctggag cactgcgccg aggacctcgc
     3661 gctgtttgcc aagtttgtgg ataaaggcct gcacgagcgg ctcaagttcg tcgtcgagcg
     3721 gcccttcgtg cgtgtgcctt actcggaggc ggtcgagatc ctgaagaagt cgggcaagaa
     3781 gttcgagttc cccgtggagt acgggctgaa cctgcagtcg gagcacgagc ggttcctcac
     3841 cgaggagcac ttcaagtccc cggccaccgt gttcaactac cctcgcgaaa tcaagccgtt
     3901 ctatatgcgc ctgaacgacg atggcaagac cgtcacggcg atggatgtgc tggtgccggg
     3961 gattggcgag gtcatcggcg gcgcgcaacg cgaggagcgc ccggagcaac tgctcgagaa
     4021 catgaagtcc cataagctca agcccgagga ttactggtgg tatgcggacc tgcgccgcta
     4081 cggcacggtg ccgcacgccg ggttcgggtt agggttcgag cggctgctca tgtttctgac
     4141 gagcgtgccg aatatccggg acgtgatccc cttcgcccgc acgccgggta acgcggactt
     4201 ctaaagccgg gtagaactca cagccactgc ttgaggcgtt cgcgcaggag attgcgggcg
     4261 cggtcaaggt cggcagcgtc agtggtgtcc atgaggggaa gacgtgtcgg gggacatcgt
     4321 gttctcgatt tgctcgtgga acccaagggt cagacgttgc atctcggcaa tccccctgga
     4381 aactcttgtc acctcgccag gtttgcctgg aaatcttgcc ggtgcgcagc ccatgggcgg
     4441 taatgcaccc tgcagccgcc ccgagagtgc ataaactgaa gaggccacca atcgagcacc
     4501 aatcgagcac caatagagga acaatagaga gtatcccgca ggcttctggc tggcaccacg
     4561 ctagcaggtc cgcctgttcc ggctcgggtc gaaggccgac ttccgcgttc aggacaggtc
     4621 atcccacgcc gcagaccctc atgcggcttt ggcggtcttc cggcgtttgg ccctggcaat
     4681 gccgcgagca aaagcctggt ccagttccgc cggtttgagc gcagtcgagt tgccactgct
     4741 cagagcctcg tgaacccagc gggcgtgaag ggcggacatc ttggcgtcct ccgcatcaaa
     4801 ggagcggttg agccgggact ggacttcttg attcaggctt cggaaatgct ctgcggcggc
     4861 cgatcgcaac cggctgtaga gatcagcggt atgtttctca gcggtagatc aggcatggtt
     4921 aaaccataac ggtatcaaaa tgactgtcaa acgcccaacg atctggcttt cacggcggga
     4981 cacggtgtcc ggggaactgt ctccgggaat cggtctagac ctcgagactg gcacacagac
     5041 cggcggacgg gataggcggg ccggaattcc tgaaagaagg tgttggcttc cgttctttct
     5101 tatggtgccg tttgcgccct ccgccccttc cgggccggct tggcggcggc gaccagcggg
     5161 gcggtgag
//
LOCUS       DATCGS010000370         6008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_478033, whole genome shotgun sequence.
ACCESSION   DATCGS010000370 DATCGS010000000
VERSION     DATCGS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478033"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..617
                     /locus_tag="VIL39_07625"
     CDS             249..617
                     /locus_tag="VIL39_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5481332.1"
                     /translation="MAAILGSYRDAIAFRPERFNPVLVASGSHMKAVLTCLEPGQFIP
                     VHHPGIDMLLIVLEGEGEVVSGDRQEAAHPGTVIFAPAGEARGVRAETRLIALHVVSP
                     PPTEKDHVEVTAGLKEGKWL"
     gene            614..1789
                     /locus_tag="VIL39_07630"
     CDS             614..1789
                     /locus_tag="VIL39_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEY5481333.1"
                     /translation="MRSFDEAPFIVIWELTQACDLACVHCRACAIESRSPLELSTEEG
                     FRLLEEVRSFGDPLMVFTGGDPLKRPDLLPLIEKSVRLGLRTTVTPSATPLLTEGAIE
                     RFRDCGLARMAISLDGPDSESHDGFRRVNGSFNCTILALEHAQRIGLQTQVNTTVTRH
                     NVGRLGEIARLVERVGAKLWSVFFLVATGRASASQDLTAEEYEEVFAFLYNLSKTAPF
                     DIKTTEAQHYRRYVAQSRKADSEGGGAARRTAPEIIQRQAGINDGKGFVFVSHTGEIY
                     PSGFLPLTAGNVRRDSLTAVYRESPLFRTLRNADNLQGKCGECEYRNLCGGSRSRSYA
                     LTGNPLAEEPRCIYQPKPMGDALPRDRRAEQAGIPPLGNDAPLRPAIHLSHVMPGDS"
     gene            complement(1761..2099)
                     /locus_tag="VIL39_07635"
     CDS             complement(1761..2099)
                     /locus_tag="VIL39_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5481334.1"
                     /translation="MTDVHVYVRDIRELAKVPENGILSQTLQNDDRTKVILFGFAAGQ
                     ELSAHTAPFPATLIFLKGEAHLRLGGEEQLATEGTFVYMPPNLEHGIKAQSEVVMLLT
                     MIKNPPASHG"
     gene            complement(2116..4437)
                     /gene="metE"
                     /locus_tag="VIL39_07640"
     CDS             complement(2116..4437)
                     /gene="metE"
                     /locus_tag="VIL39_07640"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY5481335.1"
                     /translation="MNTHSIAAGNLGYPRIGLHRELKVALERFWSGEWSSQELEAATR
                     ELRAARWRIQAEAGIVHIPSNDFSLYDHVLDAAVLVGAIPARFGRHFEDLDLPTYFAM
                     ARGSKSAPAMEMTKWFDTNYHYIVPELEPGMEFHLGSRKPVEEFLEAKALGLTTRPVL
                     LGPVSLALLGKTHGTRWDRANIAQALVGVYRQLLIELAAAGADWVQMDEPCLGLDLPE
                     EHRNLFGRIYEMLILPARSPKILLATYFSDLGENLEFTLRLPVAGVHLDLVRGPGQLA
                     QALALAPDNLLLSLGVVDGRNIWRSDLDRALEMIRMAAGRLGSERIQVAPSCSLLHIP
                     VDLEAEHQLDPGLRTWLAFARQKLDETALLARAATEDSLEVEERLAENRAILGRRHRD
                     RRACDPAVRGRTAAVRPEMFERCSPFPVRREKQTVLRLPILPTTTIGSFPQTAEVRRA
                     RAVCRSGKMARTEYEALLRAEVERAIRFQEEIGLDVLVHGEFERNDMVEYFGEQLRGF
                     AFTGNGWVQSYGSRCVKPPLLFGDVSRTSPMTTAWAHYAQSLTDLPVKGMLTGPVTLL
                     EWSFVRDDLPKSEVAVQLALALRDEVADLEGAGMRIIQLDEPALREGLPLRRAEQAGY
                     LRWSVDAFRLATSGANDETQIHTHMCYSEFADILDAVVRMDADVLSVEAARSQMELLD
                     TFNQKQYPNDIGPGVYDIHSPRVPDAEEIAALLRKALTVLRPEQLWVNPDCGLKTRGW
                     DEVRAALRNMVAATRRVREQVRVAGNAKPPRGH"
     gene            complement(4496..4900)
                     /locus_tag="VIL39_07645"
     CDS             complement(4496..4900)
                     /locus_tag="VIL39_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481336.1"
                     /translation="MAVLSTREDRAALQRIIGPCKWELQWTGTGGEAIDAFRRTSPPI
                     VICDRDLPDGDWRQLWDILAREPKPPMFIVTSRLADDALWAEVLNVGGYDLLLKPFRA
                     EEVIRMVHGAATQTPPAKANPHQASEILCRAE"
     gene            complement(5092..5358)
                     /locus_tag="VIL39_07650"
     CDS             complement(5092..5358)
                     /locus_tag="VIL39_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5481337.1"
                     /translation="MLAKMLEISKEVVAVADHNTRVAPVSPLSEQEQRILRLFAKAKN
                     SSEIARDLGITLPTLRNHLHSINEKLRTHNRLEAVMHAMQRGLI"
     gene            complement(5432..5746)
                     /locus_tag="VIL39_07655"
     CDS             complement(5432..5746)
                     /locus_tag="VIL39_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5481338.1"
                     /translation="MLEAELFAFLERTADAAFAVTREGQICSWNQAAERLFGYSSADV
                     LNRACYEVLDGHGALGTQVCIGDCSVQQCAAQKLGIPDFDLEVKTRSGKRLWVNVSTL
                     VF"
     gene            complement(5793..>6008)
                     /locus_tag="VIL39_07660"
     CDS             complement(5793..>6008)
                     /locus_tag="VIL39_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY5481339.1"
                     /translation="ACPVDCIHPKKDESEFEDAPQLYIDPQERIDCGACVPVCPVSAI
                     LALDDLPTQGSQFAQINHEHYRKASAH"
BASE COUNT         1158 a         1847 c         1771 g         1232 t
ORIGIN      
        1 aaccatcctg atggcattgc tggccgtcgc ctgcgccgag caatacttcc gctggtatgc
       61 tgcagttgcc ttcctgcggt tttttcccgc ggattcgcct gatcaaacct caaccgctcg
      121 caattcaggc cctcggtaag agcgaaccga tctacgcagc catatttggc gtttttctcg
      181 ttggttggca cgcaattgtc gtgacttgcg tcactgccgc cggtctatgg gcaccgtaat
      241 ctgaaggcat ggcggcaata ttgggttcgt atcgcgacgc aatcgcattc cggccggaga
      301 gattcaaccc ggtgctggtg gcaagcggct cacacatgaa ggctgtgctt acttgcctgg
      361 aaccgggtca gttcattccg gtacaccacc ccggaattga catgttgctg atcgttctgg
      421 agggtgaagg tgaggtggtc tcaggcgatc ggcaggaggc cgcccacccg ggaacggtca
      481 tctttgcccc tgcgggcgag gctcgtggag tcagggcgga aaccaggctg attgccttgc
      541 atgttgtgag cccgccaccc accgagaagg accacgtcga agttacggcc gggctgaaag
      601 aagggaaatg gctatgagaa gtttcgatga agcacctttc attgtcattt gggagctgac
      661 acaggcctgc gatctggcgt gcgttcactg ccgggcctgc gcgattgaga gccgaagccc
      721 attagaactg tccactgagg aaggtttccg cttgcttgag gaagtccgct ccttcggtga
      781 cccactgatg gttttcactg gcggcgaccc tttgaagcgg ccggacctgc tcccgttgat
      841 cgagaagagc gtgcgcttgg ggctgcgaac caccgtgact ccgagcgcaa ctccactgct
      901 gacggagggt gccatcgaac ggtttaggga ctgtggattg gcccggatgg caatcagcct
      961 cgatgggccg gactcggaaa gccacgacgg attccgccgt gtcaacggtt ccttcaattg
     1021 tacgattctc gcgttggaac atgcacaacg aattggcctg caaacgcagg tgaacaccac
     1081 cgtcaccagg cacaatgtcg gccgcctggg cgagattgcg cggttggtcg agcgcgttgg
     1141 cgcgaagctc tggagcgtgt tctttctggt tgcgacggga cgggcgtcgg catcgcagga
     1201 cctgactgcg gaagagtacg aagaggtctt cgccttcctc tacaatctgt cgaagaccgc
     1261 cccattcgac atcaagacca cggaagccca gcactatcgc cgctacgtgg ctcaaagcag
     1321 aaaggccgat agcgagggcg gcggcgcagc acggcggacg gcgcccgaga tcattcagcg
     1381 gcaggccggc attaacgacg gcaagggatt cgtatttgta tcgcacacgg gggaaatcta
     1441 ccctagtggt ttcctgccac tcactgcagg gaacgtccga cgcgactcgc ttacagcggt
     1501 gtaccgcgaa tcacccctct tccggacact ccgcaatgcc gacaatctgc aaggaaagtg
     1561 cggtgaatgc gaataccgta acctgtgcgg tggttcccga tcgcgttcgt atgcgctcac
     1621 ggggaatccg ctggccgaag agccgcggtg catctatcaa ccgaagccga tgggcgacgc
     1681 tcttcctcgc gatcggcgcg cggaacaggc agggatcccg cctctcggga acgacgcacc
     1741 ccttcgtcca gctatacacc ttagccatgt gatgccgggg gattcttgat catggtcagg
     1801 agcatgacga cttcactctg cgctttgata ccgtgttcca ggtttggcgg catataaacg
     1861 aaggttcctt ccgtcgccag ttgttcctca ccgcccagcc tgagatgggc ctctcccttc
     1921 aggaaaatca gcgtggcagg aaaaggagcc gtgtgcgctg aaagctcttg tcccgccgca
     1981 aagccgaaca gaatcacctt tgttctgtca tcattctgca gcgtctggct cagtatcccg
     2041 ttctcgggta cctttgccag ctcgcgaata tcgcgtacgt aaacgtgtac gtcagtcatt
     2101 cttctatcat cctcgctaat ggcctcgcgg tggttttgcg ttgccggcca ccctcacctg
     2161 ctcacggacg cgccgggtgg cagctaccat gtttcggagc gcggcccgca cttcgtccca
     2221 cccgcgcgtt ttcagcccgc aatcggggtt cacccataac tgctccgggc ggagcaccgt
     2281 cagcgccttc cggagaagcg ccgcgatctc ctcggcgtcc ggcacgcgcg gagaatgtat
     2341 atcgtagacg ccgggcccga tgtcgtttgg atactgcttc tgattgaagg tgtcgagtag
     2401 ctccatttgc gaacgcgccg cctcgacgga gagcacatcg gcgtccatgc gcaccacggc
     2461 atcgagaata tcggcaaatt cggaatagca catgtgcgtg tgaatctgag tctcgtcgtt
     2521 cgctcccgag gtggcaagcc tgaatgcgtc gacggaccag cgcaggtatc cggcttgttc
     2581 cgcccgacgc aagggcagac cctcgcgcag ggcaggctca tccagttgaa tgatgcgcat
     2641 tcccgcgccc tccaggtccg ccacctcgtc gcgcagggcc agtgcgagtt gcaccgcgac
     2701 ctcggatttc ggcagatcgt cccggacgaa cgaccattcg agcagggtca ccggcccagt
     2761 caacataccc ttgaccggga ggtcggtcaa agactgcgcg taatgcgccc aagccgtcgt
     2821 catagggctt gtccgcgaca catccccgaa cagcaaaggg ggcttcacgc agcgggagcc
     2881 atagctctgc acccagccgt tccctgtaaa ggcaaagccg cggagttgct cgccgaagta
     2941 ctccaccatg tcattgcgct cgaattcgcc gtgcaccagc acatcgagcc cgatctcttc
     3001 ctggaaacgg atggcgcgct cgacttccgc gcgcagcaaa gcttcgtact ccgtgcgagc
     3061 cattttcccg gaccggcaaa cggcgcgcgc ccggcggacc tccgcggttt gcggaaacga
     3121 gccgatcgtt gtagtgggca gaatcgggag acgcagtaca gtttgtttct cgcgtcggac
     3181 cggaaaagga ctgcaacgct cgaacatttc cggtcggacc gccgccgtgc gaccacgcac
     3241 cgctgggtcg caggctcgcc tgtcgcggtg acgccggccg agaatcgcac ggttctccgc
     3301 cagccgctcc tcgacttcca gcgagtcttc ggtggccgcc cgcgccagga gtgcggtctc
     3361 gtccagcttt tgccttgcaa acgccagcca ggttcggagg ccgggatcga gttgatgctc
     3421 tgcttccaga tcgaccggaa tgtggagcaa cgagcatgat ggcgcaacct ggatgcgctc
     3481 ggagcccagg cgcccagcgg ccatgcggat catctccaga gcccgatcca gatccgaccg
     3541 ccagatattt cggccatcca ccacgccgag cgacaacagc aggttgtcgg gcgccagcgc
     3601 cagagcttgc gcgagctggc caggaccgcg gaccaagtcc agatgcacgc ccgcaaccgg
     3661 tagccgcaga gtgaattcca ggttctcacc caggtccgag aagtacgtcg ccaggagaat
     3721 cttgggactg cgggccggta ggatgagcat ctcgtatatc cggccgaaca ggtttcggtg
     3781 ctcctcgggc aggtccagac cgaggcacgg ttcatccatt tgcacccagt ctgccccggc
     3841 cgccgccagt tcaatcaaca attgccggta cacgccaacc agcgcttgcg cgatatttgc
     3901 ccgatcccat cgcgtgccgt gagtttttcc caacagcgcc agcgagaccg ggcctagaag
     3961 cactggacgg gttgtgagcc cgagcgcttt tgcttcgagg aactcctcga ctggtttacg
     4021 gctgccgaga tggaactcca ttcccggctc gagctcagga acgatgtagt gataattcgt
     4081 gtcgaaccac ttggtcatct ccatcgccgg agcggacttg ctcccgcgcg ccatggcgaa
     4141 atacgtgggc aggtccaagt cttcgaaatg gcggccgaat cgcgcgggaa tggcgccgac
     4201 cagcacggcc gcatccagca cgtggtcgta aagcgaaaaa tcattcgacg ggatatgaac
     4261 gatgcccgcc tccgcttgaa tccgccagcg ggcggcgcgg agttcccggg tcgccgcctc
     4321 caactcctgg gagctccatt cgccagacca gaaccgctcc agggcgacct tcaactcccg
     4381 gtgcaatccg attcgcggat aaccgaggtt gcctgccgcg attgaatgtg tgttcatgtt
     4441 cggactttgc tgcagaattc acaattggaa gcgcggggaa acggcgccgc agctcctatt
     4501 cagctcggca caagatttca gatgcctggt gcgggttggc cttagctggc ggggtttgtg
     4561 tcgcagcacc atgcaccatc cggatcactt cttctgcgcg gaatggtttc agcaacagat
     4621 cgtatccgcc gacgttgagc acctccgccc acagcgcgtc gtccgccaac ctggaagtca
     4681 caatgaacat cggcggcttg ggctcgcgag ccaggatatc ccaaagttgg cgccaatctc
     4741 cgtccggcaa atcgcggtca caaatcacga tcggagggct tgtccggcgg aatgcgtcaa
     4801 ttgcttcccc gccggtgccc gtccattgca attcccactt gcacgggccg atgattcgct
     4861 gtaatgcggc ccggtcttcc cgcgtggaca gcacggccat gagcctggag cgagaatccg
     4921 cgccctttcc caagtgttcc acgagccact gtgttcgtcc ttgcggcgtc ttcgcgatca
     4981 acgatttgcc tccttttcta tactttccgg cttttgaggc aagatcacaa tgatccgttt
     5041 gtcacaggct caggaacatc cgttatgttc gcaaggcggc aaagagggcg tctagatgag
     5101 gccgcgttgc atggcgtgca tcacggcttc caggcgattg tgagtacgca gtttctcgtt
     5161 gatgctgtgc agatgatttc gcagtgtggg cagcgtgata cccaggtcgc gggcgatctc
     5221 ggaagagttt ttggctttcg cgaataggcg aaggattcgc tgctcttgtt cggagagtgg
     5281 cgagacgggc gccactcgag tgttgtggtc agcgacggcc accacttctt tggagatctc
     5341 cagcatcttg gcgagcaact cttcgctctt cttacggtgc gaaatgtctc gagccaggtg
     5401 cacaatcagg cggcggtcgc gccggggttc ttcaaaacac gagggtggaa acgttcaccc
     5461 acagcctctt gccagaacgt gtcttgacct ccaggtcaaa atcaggaatg cccaacttct
     5521 gcgcagcgca ttgctggacg ctgcaatcgc cgatacagac ctgcgttccc agggcgccgt
     5581 gaccgtccag gacttcatag cacgcccggt tcagcacgtc cgccgatgag tatccaaaca
     5641 gccgttccgc tgcctggttc cacgagcaga tctgaccttc cctggttacc gcaaatgcgg
     5701 cgtccgccgt cctctccagg aatgcgaaca gttccgcctc cagcatcccg gccttcccgc
     5761 ctcagtattg agggcttcaa ttccattatg cgtcaatgcg ctgaagcttt ccgatagtgc
     5821 tcgtggttga tttgcgcaaa ctggctccct tgggtgggca ggtcatcgag tgcaaggatc
     5881 gcggagaccg gacaaacggg cacgcacgcg ccacaatcga tcctctcctg tggatcgatg
     5941 tatagttgtg gtgcgtcttc aaattcgctc tcgtccttct tcggatggat gcaatcgact
     6001 gggcaagc
//
LOCUS       DATCGS010000371         6280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_625896, whole genome shotgun sequence.
ACCESSION   DATCGS010000371 DATCGS010000000
VERSION     DATCGS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625896"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            650..1918
                     /locus_tag="VIL39_07665"
     CDS             650..1918
                     /locus_tag="VIL39_07665"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEY5481340.1"
                     /translation="MSTTKQVEQGYRLAKERYAALSVDTDKALKQLAGIPVSIHCWQG
                     DDVGGFENAGEALGGGLAVTGNYPGKARTPDELRADFEAALAVSPGRHRFNLHACYAE
                     MGGRKVDRDELSVKQFKNWIAWARSIKIGLDFNPTFFAHPKVAGGFTLSSANKGIRQF
                     WIEHGIRSREIGAAIGKALGNTCLNNIWIPDGMKDTPADRRGPRERLAESLDAMLKKK
                     ISPKYNVDSVEPKLFGIGAESYTPGSHEFYLGYAVSRQILLTLDAGHYHPTEGIADKI
                     SSVLCYLPEIALHVSRGVRWDSDHVVTLTDELQAIAQEIVWGGNASRVRIGLDYFDAS
                     INRVAAWVIGARNMRKALLLALVAPIAKLRQLEAEGDYTSRLALMEDAKLLPAGAVWD
                     YFCARQGVPIGEAWLAEVKNYERTVLAKRS"
     gene            2067..3287
                     /gene="rhaT"
                     /locus_tag="VIL39_07670"
     CDS             2067..3287
                     /gene="rhaT"
                     /locus_tag="VIL39_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEY5481341.1"
                     /translation="MNPFLGVFFHWLGGLASGSFYVPYKGVKKWAWEVYWLVGGFFSW
                     IICPVLLASLLTNDVFGVLKQQSLGTLWWTYFFGAMWGFGGLTFGLTMRYLGMSLGMG
                     VALGYCAAFGTLLPPIFKTFIPTIPVAETIGQIASTTPGKVTLFGVFVCLVGIVVAAL
                     AGLTKEKEMPEAEKKKAIAEFSFTKGILVATFSGIMSACFSFALTAGNPIGEASRAAG
                     TTVIWSGLPKLVVVLLGGFTTNFIWCSFLNLKNRTGYQYFSSTLRNNLPVRNQETIIE
                     TALDAPSEEAVEQAPKIAVAGNHQVPWLSNYLFSALAGTTWYFQFFFYTMGETQMGKF
                     GFASWTLHMASIIIFSTMWGWIFHEWKGASKKSHGLIASGIAMLILSTLVIGYGTLLK
                     SGQTLGEVIKMIFG"
     gene            3300..4772
                     /locus_tag="VIL39_07675"
     CDS             3300..4772
                     /locus_tag="VIL39_07675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEY5481342.1"
                     /translation="MPHKVYLAIDLGAESGRVMAGLWNGKTIRLEEVHRFPNGPVYLG
                     DSLRWDVVRLWAEIQSGLGLAAKKYGKSIVSVGADTWGVDYVLLNRHDEMLGQPYAYR
                     DARTNGMMERAFRKVPRSEIFAQTGLQFMQFNSLFQLLAAKQHTVELLDATDCLLFMP
                     DFIHWALCGSRVVEFTIASTSQCLNPLTRNWATGLLKKFGLPARMFPEVVPPGTKLGT
                     LRPGVADRTGLRKVNVVAPPTHDTASAVAGVPTANTGKANRAYLSSGTWSLMGAEVNK
                     ASLSARTQELNLTNEGGLDGTYRLLKNIMGLWLVQQCKRAFDARGRKYEYGQLAQMAA
                     KAPALRSIVNPDDSRFLNPPDMPKAIQDFCRETKQPVPKTEGELVRCAYESLALKYRE
                     VLGWLEELTGNRIEVIHIVGGGSKSAILNQFTADACQRPVITGPVEATAMGNLLVQVR
                     ASGELASLAEMREVVRKSSDVATYRPGKPAAWEAASGRFA"
     gene            complement(4803..6020)
                     /locus_tag="VIL39_07680"
     CDS             complement(4803..6020)
                     /locus_tag="VIL39_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY5481343.1"
                     /translation="MNGSKNTNDAVISELCPVCGLCCNGVIFADVRLQPADDAGRLRS
                     LGLPVCTPRSGLRPPRLAQPCAAFDGCRCRVYPDRPQYCREFECVLFKSVKAGRTEPG
                     AALRIIRTARERADKVRRLLRKLEDADEQLPLSARFRRSGKRLQECDLDEETADLYAQ
                     LTLAVHDLNLLLGDAFYPGSASQKPKSGALRTAGALGCAALLLFVAADWLLADQVEMQ
                     NGDRYAGQVLSLNTNIVVFQSDVLGTVQLPRAKVAVITLGAGSATNSPALPSLTNAQV
                     SAPSATPANAPPNLSPALRQLGANTNLIQQVQKQFLSGAGPEANTKFNDLLGGLMSGR
                     LSVDDIRAEAKSAADQLRALQRESGEDAGFATGAYLAILDHFLKETAPSGSASNSSGS
                     LPKSNSQSNQDEN"
BASE COUNT         1231 a         1957 c         1940 g         1152 t
ORIGIN      
        1 catcccacat ccaaacccga acaccgctgc acggcgggag agccgcagga gagtgatttg
       61 gataagcccg gctgatcaaa aacgggcgtt tgaggggggc tatgggtata ggacaggtcc
      121 agggtggtac cagagtgccc ccagaccagc cccagggagg ggagggaggg aagttgtatt
      181 cggaagactt atcgtcaaca ccgaacatcg aacatcgaac atccaacttc caacatcgaa
      241 aggccgggcg gagccaccca aagccggagg tgattcgtga ttcgtgattc gtgactgggc
      301 gggcaagcca ccccaaagcc acatcaatgc gacatcaaag ccaccccaag gcctgttgat
      361 agccaactga taggcacccc aaggccaccc caaggccacc ccaaagccac cccaaagctc
      421 cctcgaagcc acttcaagct gaacgctgcg gagacagcct ggtccctggg ttccaggctc
      481 gggccagcgg ttggaaggtg ctggcctcgg ggttgaagcg gctctccgaa aatccggggg
      541 cagaatatct gctgaggccg gctcaagccg gttgttgcgc agtgcggtcg aggctgttag
      601 cattccgccg gactgaagat caaagagcac cttaaatatg actgtaagca tgagcactac
      661 taaacaagtc gaacaaggct atcgcctcgc caaggaacgc tatgccgccc tgagcgtgga
      721 cacggacaaa gccctcaaac aactcgccgg gatcccggtt tccatccatt gctggcaggg
      781 ggatgatgtc ggcggctttg agaacgccgg cgaagcgctg ggcggcggac tcgccgtcac
      841 cggcaactac cctggcaagg cccgcacccc cgacgagctg cgggcggact ttgaagcggc
      901 gctggccgtc agtcccggac gccaccgttt caacctccac gcttgctacg ccgagatggg
      961 cggccgcaag gtggaccgcg acgagttgag cgtcaaacag ttcaagaatt ggatcgcctg
     1021 ggctcgctcc atcaagatcg gcctcgactt taacccgacc ttcttcgcgc atcccaaggt
     1081 cgcaggcgga ttcacgctca gcagcgctaa caagggcatt cgtcaattct ggatcgagca
     1141 cggcattcgc tcccgggaga ttggcgctgc catcggcaaa gccctcggca acacctgctt
     1201 gaacaacatt tggattcccg acggcatgaa ggatacgccc gccgaccgtc gtggtccacg
     1261 cgagcgcctg gccgagtcgc tcgacgccat gttaaagaag aagatcagcc cgaagtacaa
     1321 tgtggactcg gtggagccta agctgttcgg cattggcgcg gagagctaca ctcccggctc
     1381 ccacgagttt tacctgggct acgctgtcag ccggcaaatc ctgctcacgc tggacgccgg
     1441 ccactaccac ccgacggaag gcatcgcgga taagatctcc tcagtgctct gctacctgcc
     1501 ggagatcgcc ctgcacgtca gccgcggggt gcgctgggac agtgaccatg tggtcacgct
     1561 caccgacgag ctccaggcca tcgcgcagga gatcgtttgg ggtggcaatg ccagtcgcgt
     1621 gcgcatcggc ctcgattact tcgacgccag catcaaccgc gtcgccgcct gggtcatcgg
     1681 cgcgcgcaac atgcgcaagg cgctcctgct cgcgctggtc gcccccatcg ccaagctcag
     1741 gcagctcgaa gccgagggcg actacacctc gcgcctggcc ctgatggagg acgccaagct
     1801 cctgcccgcc ggtgccgttt gggattattt ctgtgcccgc cagggcgtgc ccataggcga
     1861 agcctggctc gcggaagtga agaactatga gcgcacggtt ctcgcgaagc gaagctagta
     1921 tagtgacaca gttacatcgt tacatgagtt acatgaggcg tcgcaccgcc gggccttgta
     1981 accatgtaac tgatgcaact catgtaacct ttttaaccgg tggaaactcc agacgaagta
     2041 tcaaactcaa cgactaatcc gacactatga acccattcct cggcgtattc tttcactggc
     2101 tcggcggtct tgcctccggc agcttctacg ttccctataa gggagtcaaa aagtgggcgt
     2161 gggaagtcta ttggctcgtg ggcggcttct tctcctggat catctgcccg gtgcttctgg
     2221 cgtcgctgct caccaacgat gtgttcgggg tgctcaagca gcaatcgttg ggcaccctct
     2281 ggtggaccta tttctttggc gccatgtggg ggtttggcgg cctgaccttc ggcctgacga
     2341 tgcgctacct cggcatgtcg ctggggatgg gagtggcgct gggctattgc gcggcattcg
     2401 gcacgctcct gccgccgatc ttcaagacct tcatcccgac cattcccgtg gcggaaacca
     2461 tcgggcaaat cgcttccacc actccgggca aagtgacgtt gttcggcgtg ttcgtttgct
     2521 tggtcggcat cgtcgtggcc gcgttggccg ggttgacgaa ggaaaaggaa atgcccgaag
     2581 cagagaagaa gaaggcgatc gcggagttca gcttcaccaa gggcatcctg gtagcgacct
     2641 tctccggcat catgagcgcc tgtttctcct tcgcgctcac ggcgggcaac cccattggcg
     2701 aggcgtcgcg cgccgcagga accacggtca tctggagcgg gttgcccaag ctcgtggtgg
     2761 tcttgttggg cggcttcacc actaacttca tctggtgctc cttcttgaac ttaaagaacc
     2821 gcaccggcta ccagtatttc agcagcacgc tgcgcaacaa ccttccggtc cgcaaccaag
     2881 agaccattat cgaaaccgcc ctggacgctc ccagcgaaga agcggtggag caggccccga
     2941 agatagcggt cgcgggcaat catcaagtcc cttggctcag caactacctg ttcagcgcgc
     3001 tggccggcac gacgtggtac ttccagttct tcttctacac gatgggggag acacagatgg
     3061 gcaagttcgg gttcgcgagc tggaccctgc acatggccag catcatcatc ttcagcacca
     3121 tgtggggctg gatcttccat gagtggaagg gcgccagcaa gaaatcccac gggctgatcg
     3181 cctctggcat agccatgctg attctctcga ccctcgtcat tggatacgga accctgctca
     3241 agagcggtca gacgcttggc gaggtgataa agatgatctt tggctaagtc gtattcacga
     3301 tgccacacaa agtttattta gccatcgacc tcggcgctga aagcggccgc gtcatggccg
     3361 gcctctggaa cggcaaaacc attcgtctgg aggaagtcca ccgtttcccc aacggcccgg
     3421 tgtacttggg cgactcgctc cgctgggatg tcgtgcggct ttgggccgag atccagagcg
     3481 gtctcggcct cgccgctaag aagtacggca agtccatcgt ttcagtgggc gcggacacct
     3541 ggggggtgga ctacgtgctg ctcaaccgcc acgacgagat gctggggcag ccttacgcct
     3601 accgcgatgc ccgcaccaac gggatgatgg aaagggcctt ccggaaggtg cctcgctccg
     3661 agatcttcgc ccagacgggc ttgcagttca tgcagttcaa ctcgctcttc cagttgctgg
     3721 cggctaagca gcacacggtc gagttgctcg acgcaactga ttgcctgctg ttcatgcccg
     3781 acttcatcca ttgggcgctg tgcggctcac gtgtcgtcga gtttaccatc gcctccacct
     3841 cgcagtgtct caatccactc acacgcaact gggcgaccgg cctgctgaag aagttcggcc
     3901 tgcctgcgcg gatgttcccg gaggtcgttc cgcccggcac taagctcggt acgctgcgcc
     3961 ccggagtcgc ggaccggacc gggctccgca aggtcaatgt ggtggccccg cccacccacg
     4021 acacggcttc ggccgtcgcc ggcgtcccca ccgccaacac cggcaaggcc aaccgggctt
     4081 acctgagttc cggcacctgg tcgctgatgg gcgccgaagt caacaaagcc tccttgtccg
     4141 cccgcaccca ggagctgaat ctgaccaacg aaggcgggct ggacggcacc tatcgcctgc
     4201 tcaagaacat catgggcttg tggctcgtcc agcagtgcaa gcgcgccttc gacgcccgtg
     4261 gccggaaata cgaatacggg cagctcgcgc agatggccgc caaggccccc gcgctccgca
     4321 gcatcgtcaa cccggacgac tcgcgcttcc tcaacccgcc cgacatgccc aaggccatcc
     4381 aggacttttg ccgcgaaacc aaacagcccg tgccgaagac cgaaggcgag ctggttcgtt
     4441 gcgcttacga aagcctcgcg ctcaagtacc gcgaagtgct gggctggctt gaggaactca
     4501 ccggcaaccg cattgaggtc attcacattg tcggcggcgg ttccaagagc gccatcctta
     4561 accagttcac ggctgatgcc tgccagcgcc cggttatcac gggtcccgtt gaggcgaccg
     4621 ctatgggcaa cctgctcgtc caggtgcgtg ctagcgggga gttggcttcc ctggccgaga
     4681 tgcgggaagt cgtccgcaag tctagcgacg tggccactta ccggcccggc aagcccgccg
     4741 cttgggaagc cgcctccggc cgattcgcgt agctgcgccg cggcaaggga ccctatccca
     4801 catcagttct cgtcttgatt actttgtgaa ttggacttcg gcaacgaccc cgagctgttt
     4861 gaggcggaac ctgatggcgc ggtttccttg aggaagtggt cgaggatggc caggtaggca
     4921 ccggtggcaa aacccgcatc ctcaccggat tcgcgctgga gcgcgcggag ttgatcggcg
     4981 gcggatttgg cctcggcccg gatgtcgtcc acgctcagtc ggccgctcat taagccaccg
     5041 agcagatcat tgaacttggt gttcgcttcg gggccggcgc cgctgaggaa ctgcttctgg
     5101 acttgctgga tgaggttggt gttcgcaccg agctggcgga gtgccggaga cagattgggc
     5161 ggcgcgttcg ccggcgttgc cgagggtgcg ctaacctggg cgttggtcag cgagggcaga
     5221 gcaggggagt tggtcgccga accggcccca agcgtaatga cggccacttt ggcgcggggc
     5281 agttgcaccg ttcccagcac gtcgctttgg aagacaacga tattggtgtt aagcgacaag
     5341 acctggccag cgtaacggtc gccgttctgc atttcgacct ggtcggccag cagccagtcg
     5401 gcggcgacga atagaaggag ggcggcgcaa cccagcgcac ccgcggttcg taaggcgccg
     5461 gacttcggtt tctgggatgc gctgccggga taaaaggcgt cccccagcaa caggttgagg
     5521 tcgtggacag ccagggtgag ctgggcataa aggtcggccg tttcttcgtc cagatcgcac
     5581 tcttgcaggc gctttccgga gcggcggaag cgggcgctga gggggagctg ctcatccgca
     5641 tcctccaact tgcggagcag gcggcggacc ttgtcggccc gctcgcgcgc ggtgcggatg
     5701 atgcgcaggg ccgcgccggg ctccgtgcga ccggccttga cactcttgaa gaggacgcat
     5761 tcgaactcgc ggcagtactg ggggcggtct gggtaaacgc ggcaccggca gccatcgaac
     5821 gcggcgcaag gctgcgcgag gcgcggcggg cgcagccccg agcgcggggt gcaaacgggc
     5881 aggccaagcg accgcagccg gcccgcatcg tcggctggct gcaaccggac atcagcaaag
     5941 atgacgccat tgcagcaaag cccgcagacg gggcagagct cgctgataac tgcgtcgttc
     6001 gtattcttcg agccgttcaa actaaccgaa agctaaggcc aaacgccctc taaggcaatg
     6061 gggctggtgg gatccagcgg tactgccgga gcagcgctgg ggacggcgga gcgcggcctg
     6121 aaggccgcgc tccagtccac cggtggggcc tcgctgatgc gctacgcttg tgtaatctca
     6181 aagtctctcc gctagtccac cgtagaatcc tgttgctgaa caccaaacgc gggaatacgc
     6241 gcgccccaag acgcctgata atgcgtccaa tacagggccg
//
LOCUS       DATCGS010000372         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_427190, whole genome shotgun sequence.
ACCESSION   DATCGS010000372 DATCGS010000000
VERSION     DATCGS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427190"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VIL39_07685"
     CDS             complement(<1..259)
                     /locus_tag="VIL39_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481344.1"
                     /translation="METERICPSCHKPLPPDVPLGLCPECLMKAGLNTGTEPGVEGST
                     GSGFVPPPVADIAKLFPQLEVLEFIGKGGMGAVYKARQPSLD"
     gene            complement(408..1229)
                     /locus_tag="VIL39_07690"
     CDS             complement(408..1229)
                     /locus_tag="VIL39_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481345.1"
                     /translation="MSTSEPTAAPGTTDQADGLPRSPVFATTHWSVVLTAGGSDTAGA
                     RDALAKLCQTYWYPLYAYVRRRNLSPPDAEDLTQEFFARFLEHHWVANADREKGRFRT
                     FLLSAMNHFLANEWDKARAQKRGGGVPLLPLEFDTAETRYVREPADNVTPEQHFERRW
                     AMTLLEIVVNRLQAEYELDGKAELFAALNPCLVGDRTSQPYEALARKLGVTEGAVKSA
                     VHRLRQRYRQLLRDEIAHTVAGPVEVEEELRHLIAVLGGRASERGHSCPQQLPDA"
     gene            1374..2195
                     /locus_tag="VIL39_07695"
     CDS             1374..2195
                     /locus_tag="VIL39_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY5481346.1"
                     /translation="MIKDVSAVSNDTNNRISRRTALGLGASVAAGVLLSPEWLTAAPA
                     KRKVVVWSEGTANVDSGSKEVYPNDINTAIAEGLQPLAASGWEIVKANLNEPDQGISD
                     ALLNSTDVLIWWGHKKHGDVKDELVGKIVERVKAGNMGFIATHSSHFSKPLKKLLGTA
                     CSWGEYKADGTSVEILVKEPNHPICKGVKDFKLPKIERYGEPFAVPTPEAVPLDGVYT
                     RPDGQTQPGRMGLCWTIGKGKVFYFTPGHETYDDYYRPEVRRIFINAVEWAAPGK"
BASE COUNT          454 a          743 c          696 g          426 t
ORIGIN      
        1 ggtccaggct gggctggcgg gccttataga cggcgcccat gccgcccttg ccgatgaact
       61 caaggacttc gagctgggga aacaacttgg cgatgtccgc gaccggcggc ggcacgaacc
      121 ccgacccggt ggagccttcg acccctggtt cggtgcccgt gttaaggccc gccttcatca
      181 ggcactccgg acagaggccc aggggcacat ccggcggcag cggtttgtgg cagcttgggc
      241 aaatgcgttc ggtttccata atcatgttct cgtcacctgt tccttaagga acggcgccag
      301 aaggttgccc gaaaagaatt ggcgcatcct gacaccgccg ccggagcgcg gacattcctg
      361 tccgcagcag ccttgaaagt gaacaagcgc gtggacaccc gacattgcta agcgtccgga
      421 agttgctgcg gacaggaatg tccgcgctcc gaagcccgcc cgcccagcac ggcaatcagg
      481 tgccgcaatt cctcttccac ctccactggc ccggcgacgg tgtgcgcaat ctcgtcgcgc
      541 agcagttggc ggtagcgctg gcgcaaccgg tgcaccgcgg atttgacggc gccctcggtg
      601 acgcccagtt tcctggccag cgcctcatag ggctgcgagg tgcggtcccc gaccaaacat
      661 ggattcaacg cggcgaacag ttccgccttg ccgtccagct cgtattccgc ctgcaggcgg
      721 ttcaccacga tttcaagcag cgtcatggcc cagcgccgct cgaaatgctg ttccggcgtc
      781 acgttgtcgg cgggttcccg cacataacgg gtctccgccg tgtcgaactc gagcgggagc
      841 aggggcacgc cgcctccgcg cttttgcgcc cgggctttgt cccattcgtt cgcgaggaag
      901 tgattcatgg cggacagcag gaaagtgcga aagcgccctt tctcgcggtc ggcgttggcc
      961 acccagtgat gctccagaaa ccgcgcgaag aattcctggg tgaggtcctc cgcatcgggc
     1021 ggcgagaggt tccgccggcg cacataagca taaagggggt accagtaggt ctggcataac
     1081 ttcgccaatg cgtcccgggc cccagccgtg tcgctgcccc cggcggtgag taccaccgac
     1141 cagtgcgtcg tggcgaagac cggcgagcgg ggcaatccat ccgcctggtc tgtggttcct
     1201 ggtgctgcgg ttggctctga cgtgctcacg aggccggagc ctagccgaac accgcaagac
     1261 cgcaagtgtc cacgtctatg ctggctctcc cgccttcatc tgaggcaccg tagcccttgc
     1321 gttttgtcct ctctacgctt gccagaaagc ccgagaatcg cgattatcgc gccatgatta
     1381 aagacgtctc tgctgtatcg aacgacacga acaaccgcat ttcccgccgt actgcgctgg
     1441 gcctcggcgc ctccgtggcg gcgggcgtgt tgctaagtcc tgaatggctc accgccgcgc
     1501 ccgccaaacg caaggtggtc gtctggtcgg aaggcaccgc gaacgtggac tccggctcga
     1561 aagaagtcta tcccaacgac atcaacaccg ctatcgctga ggggctccag ccgctcgcgg
     1621 ccagcggatg ggagatcgtc aaggctaacc tcaatgaacc cgaccagggc attagcgatg
     1681 ccctcctgaa cagcactgac gtactaatct ggtggggcca caagaagcac ggcgatgtaa
     1741 aggacgaatt ggtgggcaag atcgtcgagc gcgtgaaagc ggggaacatg ggcttcatcg
     1801 ccacccattc ctcgcatttt agcaaacccc tcaagaagct cctgggcacc gcctgcagtt
     1861 ggggcgaata caaggccgat ggcacctcgg tcgagatcct cgtcaaggaa cccaaccatc
     1921 caatctgcaa aggagtgaag gatttcaagc tgccgaagat cgagcgctac ggcgagccgt
     1981 ttgcggtgcc gacgcccgaa gccgtgccgc tcgatggcgt ttacacccgg cccgatggcc
     2041 agacccaacc cggccgcatg ggcctctgct ggaccatcgg caaaggcaaa gtcttctact
     2101 tcactcccgg acacgagact tacgacgact attaccgccc cgaggtgagg cgcatcttta
     2161 tcaacgccgt ggaatgggcg gcgccgggca aataggcaaa ctccaagtcc aataattgtt
     2221 cgcagtgtgc aatctatcat tatgaataca tcatcgaaac tatactcccg ccgggaagcc
     2281 ctgggggcga tggggctctt tgcgcttgct tgccatcct
//
LOCUS       DATCGS010000373         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_725005, whole genome shotgun sequence.
ACCESSION   DATCGS010000373 DATCGS010000000
VERSION     DATCGS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725005"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="aroB"
                     /locus_tag="VIL39_07700"
     CDS             <1..880
                     /gene="aroB"
                     /locus_tag="VIL39_07700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY5481347.1"
                     /translation="TAKSLKTLEACYGLLAAQRLERKSFIVALGGGVVGDLAGFVAAT
                     YLRGLPFVQVPTTLLAQVDSSVGGKVGLNLKAGKNLVGAFHQPRLVLCDLDTLASLPM
                     REYRAGLAEVIKYGIIYDARLFEHLERDLPKLLRRDPRALAAVIARCCEIKADVVRQD
                     ETESGLRAILNFGHTIGHALETTSHYGKYLHGEAIAIGQVAAAKLSAQLLGLPQHDVQ
                     RIERLFQNAGLPTHVKLSAPQRQKILTAMSLDKKVSDGVIKFVLARRIGAVEFGQLVP
                     TDLLAGAINHQPSTIQ"
     gene            884..1510
                     /locus_tag="VIL39_07705"
     CDS             884..1510
                     /locus_tag="VIL39_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481348.1"
                     /translation="MSAVSETIVREYFELHEFLVRQHCKYISPAHREEDDDIDFFVLN
                     PHPQVPAETLPFVLGSPDLAFIERAIVVVKGWHTETFSTSRLESSPAIFRFVEPKVFQ
                     QAARAFGQDGTPLKILVVPALPQAAEARDGSIALLRSKGIDGVIPFHTMLADLVKETE
                     VNRNYQKSDLLQIIRILKNYDFFKEPQLELFRTKRKKQAQSKTQKAQA"
     gene            1588..2154
                     /locus_tag="VIL39_07710"
     CDS             1588..2154
                     /locus_tag="VIL39_07710"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5481349.1"
                     /translation="MVLVIDNYDSFTYNLVQYLGEMQVELQVHRNDRISIEQIRALAP
                     ERILISPGPCSPRESGLSNEIIRTFGPRIPTFGVCLGHQCIGHVFGANVIVNYRMMHG
                     KTSLIKHNGKDLFAGMPNPFAATRYHSLVIQRDSVPDCLEVTAETEEGEIMGVRHKQY
                     PIWGVQFHPESILTQNGRQILKNFLRLN"
     gene            complement(2235..>2423)
                     /locus_tag="VIL39_07715"
     CDS             complement(2235..>2423)
                     /locus_tag="VIL39_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY5481350.1"
                     /translation="METKEAADAAVKALNGKDIDGRALTVNEARPREDRPPRSGGGGG
                     GGGYRGGGGGGGGREYSR"
BASE COUNT          498 a          794 c          672 g          459 t
ORIGIN      
        1 aacggccaag agcttgaaga ccctcgaagc gtgctacggg cttttggcgg cgcaacgatt
       61 ggagcgcaaa tcattcatcg tcgcactcgg cggtggcgtg gtgggcgatc tggcgggatt
      121 tgtggcggcg acctatctgc gcggcttgcc gttcgtgcag gtgccgacca ccctgctcgc
      181 acaagtggac agctccgtag gcggcaaagt cgggctgaac ctcaaggcgg gcaagaacct
      241 cgtgggtgcc ttccatcaac cccggttggt gctctgcgac ctggacaccc tcgcatcttt
      301 gcccatgcgc gaataccgcg cgggcctcgc cgaagtgatc aagtacggca tcatttacga
      361 cgcccggctc tttgaacact tggaacgtga tctgccaaag ttgctgcggc gcgacccgcg
      421 cgcgctggcc gccgtcatcg cccgctgctg cgagatcaaa gcggatgtgg ttcggcagga
      481 cgagaccgag agcggcttgc gggccatcct caatttcggc cacaccatcg gccacgccct
      541 agaaaccacc tcccactacg gcaagtatct gcacggggaa gcgattgcca tcggccaggt
      601 cgccgcggcg aaactctcgg cgcaactcct cggcctgccc cagcacgacg tgcagcgcat
      661 cgaacgactc ttccagaacg caggtctgcc aacccatgta aagctgagcg cgcctcagcg
      721 gcagaagatt cttaccgcca tgagtctcga caaaaaggtc agtgacggag tgatcaagtt
      781 cgtgctcgcc cgcagaatcg gcgccgtcga attcggccaa ctggtcccca ccgaccttct
      841 tgccggggcc atcaaccatc aaccatctac tattcagtag cccatgtccg ctgtcagcga
      901 aaccattgtt cgcgagtact tcgagctgca cgaattcctc gtgcgccagc attgcaaata
      961 catcagccca gcacaccggg aggaggacga cgacattgat ttcttcgtgc tcaaccccca
     1021 tccgcaagtg ccggcagaga cgctcccgtt tgtgctcggt tcccccgatc tggcctttat
     1081 cgaacgcgcc attgtggtcg ttaaaggctg gcataccgaa accttcagca cctcacggct
     1141 ggagtcatcg ccggccatct ttcgctttgt cgagcccaag gtctttcagc aggccgcgcg
     1201 cgcctttggc caggatggca cgcccttgaa gatcctggtt gtccccgccc tgccgcaggc
     1261 cgcggaagct cgcgacggga gcattgccct gctccgctcc aagggtattg acggcgtcat
     1321 accgttccac accatgctcg ccgacctggt gaaagaaacc gaggtcaacc gcaactacca
     1381 gaagtccgac ctgctccaga tcattcgcat cctgaagaat tacgacttct tcaaagaacc
     1441 ccagttggag ctattccgga cgaaacgcaa gaagcaggcg cagagcaaga cccagaaagc
     1501 acaggcctga ctcccccagc ccggccggcg ggcgatttgc tgatttccaa acccgtgctt
     1561 atgccgtaga gtcgtcctca aaccaatatg gtgctggtca tcgacaatta cgattcattc
     1621 acctacaacc tggtgcaata cctgggcgag atgcaggtgg aactccaggt ccatcgcaac
     1681 gaccgcatct cgattgagca aatccgggcc ctggcgccgg agcgcatcct gatctcgccg
     1741 gggccgtgct cgccgcgtga atcagggttg tcgaatgaaa tcatccgcac cttcggcccg
     1801 cgcatcccga cgttcggcgt gtgtctgggg caccagtgca taggtcatgt gttcggcgcg
     1861 aacgtgatcg tgaactaccg catgatgcac ggcaagacct cgctcattaa gcacaacggc
     1921 aaggacttgt tcgcgggcat gcccaatccc ttcgccgcca cccgctacca ttcactggtc
     1981 attcagcgcg acagcgtgcc ggactgcctg gaagtcaccg ccgagaccga ggagggtgaa
     2041 atcatgggcg tacggcataa acaatacccg atctggggcg tgcagtttca tcccgaaagc
     2101 attctgaccc aaaacggccg gcagatcctg aagaacttct tgcggctgaa ttagctccgc
     2161 ctaacattaa ggcgcaggat cctttcgggt cccgcgcctt gacagaatgc gttccgagaa
     2221 cgctgaagaa gtgcttagcg gctgtattcg cgacctccgc caccgccgcc gccaccgcga
     2281 taaccaccac caccgccacc gccgccggag cggggaggac gatcttcgcg cgggcgcgcc
     2341 tcgttaaccg tcagcgcccg gccatctatg tctttgccgt tgagcgcctt aacggcggca
     2401 tcggcagctt ccttggtctc cat
//
LOCUS       DATCGS010000374         4727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_81435, whole genome shotgun sequence.
ACCESSION   DATCGS010000374 DATCGS010000000
VERSION     DATCGS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81435"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..858
                     /locus_tag="VIL39_07720"
     CDS             49..858
                     /locus_tag="VIL39_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481351.1"
                     /translation="MILLAAGLQRHLALDRIRMPTGVPFFRAVASLGVPGLGFYLRGP
                     RFWGLAAMGFAALMALVFIVGLGYPVGNVAFGLLLSTHVSSVIYVFEPWLAGARFRVR
                     LSFSFVMLVLLGALVYLPVRNLIQEQWLMPLQVKGRVVIVHRLANAPALPRGAWIAYT
                     IDSARGQGLYAQEGFGLGPVLALPGDRVRFTKSALEVNGVPQPLLAYMPQVGEWVVPQ
                     KHWFVWPEVVISGHGNTPADAISATLLQMGTITEDRFVGRPFKRWFGRRQL"
     gene            861..1637
                     /locus_tag="VIL39_07725"
     CDS             861..1637
                     /locus_tag="VIL39_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481352.1"
                     /translation="MSRFVNLELGGESEDQWHQQKPAVKDEGYYLAQARAAFETADFE
                     LALRAFCKVLEFNPQNAAAWAGQVRMLIELGEHREAKLWADKALERFPHEPELLAAKA
                     VALGRSGDLQGAMAFSDAAIEERGDTPYVWLARGDVLLAREEARAAYCFEKALLLAPG
                     DWFIAWLAARIRYYYKQFVLALKLLQQALDLNPAHFFLWLQFGQCQQALGLIGPARIS
                     FAQARQLNPKCQEAAFALTRLSDSGLGSRLRGWWRRLFTR"
     gene            1701..2762
                     /locus_tag="VIL39_07730"
     CDS             1701..2762
                     /locus_tag="VIL39_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5481353.1"
                     /translation="MSSENLDRLLSAAAEFDASDLHLVVGVPPAFRVNGEIIIADEDA
                     LTEAEVTQIADHLLNEQQKKKFEQEWELCISLLHPAAGRVRATFYRRNGHPEMSFRFC
                     GDRIATREDLGLPEKIDDLSRKPNGLVLITGPTGAGKTTTLNYMVDLINSERRCKIVT
                     IEDPIEFVHANKRAIVVQQEVLSDVRSFNRALIHVLRQDPDVIVVGEMRDFEAISTAL
                     TAAETGHLVLATMHSPNVSHALERIVGVFEGSAQRQIILQLSNALQGIIAQELLPAAD
                     RTRRVLAYELIVANGAVRNLIRENQVHQLENTIQTGRKEGMTLMDNCLYDLYCKCLIT
                     YDTAVSRARHPDRIIPQKT"
     gene            2948..4168
                     /gene="bamD"
                     /locus_tag="VIL39_07735"
     CDS             2948..4168
                     /gene="bamD"
                     /locus_tag="VIL39_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY5481354.1"
                     /translation="MTHHTYQIVRGAALLAIALSFIGWVMVRWLKRTDDPARLIFKWI
                     LTVPVVVLLIFWVGPFATSSEGGAFIGVPLAAACGIVLAIIWRHNIADLIAKPFASLY
                     DGGSQEPIPHPAYSVAQSKQKRGNYLEAIAEIRKQLDRFPTDVEGQLLLAQIQAEDLK
                     DLPAAELTLEHFCAQPGHAPQNIAFALYSLADWHLKVGQDREAARRDLEKIIELFPGS
                     EFALGAAHRIAHLGSLDMLLSPHERKIFAVPEGVQNLGLLRDQDHLKPAESDPEQVAA
                     ECVKHLEQHPLDTEARERLAVIYADHYGRLDLAAGELEQMIEQPNQPGRLVVHWLNVL
                     ADLQIRSGADCETVRHTLERIVDRNPDVAAAEIARHRLALLKLQMKPKEKGQAVKLGS
                     YEQNIGLKRGSPRR"
     gene            complement(4154..4552)
                     /locus_tag="VIL39_07740"
     CDS             complement(4154..4552)
                     /locus_tag="VIL39_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481355.1"
                     /translation="MKIKMLLVLLGSLVLVAGCVSTVNDRHAFALSPGKDKFEGRYQR
                     TPEQVYAAAVEVIKFNGTITRETTINPGPGQVRAIEGKVSERSVWVSVQAVDPTVTSV
                     TVQVRTSGGGTDLTLTQELQKQIAIKLAAG"
BASE COUNT          857 a         1456 c         1479 g          935 t
ORIGIN      
        1 cgtgtgacat catactacta cccgccacgg gcgcgctggt acagcccgtt gatcctcctg
       61 gccgccgggt tgcaacgaca cctggcgctg gaccgcatcc ggatgccgac gggcgtgcct
      121 ttcttccgag ccgtggcgag ccttggggtg ccggggctgg gcttctatct ccgcgggcct
      181 cgattttggg gcctggcggc catgggattc gccgctctga tggcattggt cttcatcgtc
      241 gggctggggt atccggtcgg caatgtcgct ttcggcttgc tcctgtccac ccacgtcagc
      301 agcgtgatct atgtgttcga gccttggttg gccggcgcaa ggtttcgcgt gaggctgtca
      361 ttctcattcg tcatgctggt cctgttgggc gctctggttt atctgccggt gcgcaatctt
      421 atccaggagc agtggctgat gcctttgcaa gtcaagggcc gggtcgtgat cgtgcatcgg
      481 ctggctaacg caccggcgct gccacgcggc gcctggatcg cctataccat cgatagtgcg
      541 cgcggacagg ggctgtatgc gcaggaaggt tttggcctgg ggccggtgct ggcgctgccc
      601 ggagaccgag tgcgcttcac caaatccgct cttgaggtaa acggcgttcc tcagccgctc
      661 ctggcctaca tgcctcaggt tggcgagtgg gtcgtgcccc aaaaacactg gtttgtatgg
      721 ccggaagtcg ttataagtgg ccatggcaat acgcccgccg acgcaatttc ggcgaccctt
      781 ttacaaatgg gcactataac ggaagaccga ttcgtgggga gaccctttaa gcgttggttt
      841 gggcgtcggc aactgtaagc atgagccgtt tcgtcaatct ggagttgggc ggcgagtccg
      901 aagaccaatg gcatcagcaa aagcccgcgg tcaaggatga aggctattat ctcgcccagg
      961 cgcgtgcggc tttcgagacg gctgacttcg aattggccct ccgggcgttt tgcaaggtcc
     1021 tcgaattcaa tccccagaac gccgccgcct gggctgggca ggtccgcatg ctgatcgaac
     1081 tcggcgaaca ccgcgaagca aagctctggg ctgacaaggc cctggaacgg tttccccatg
     1141 agcctgaatt gctcgccgcc aaagcggtag ccctcggacg gagcggcgat ctccagggcg
     1201 cgatggcttt ctcggacgcc gccatcgagg aacgcggcga cacgccctat gtctggctcg
     1261 cccggggcga cgtgctcctg gcgcgggaag aggcaagggc ggcttactgt tttgagaagg
     1321 cgctgctgct agcgcccggg gactggttca tcgcctggct ggccgcgcgg atccgttact
     1381 actacaagca atttgtgctc gccctgaagc tgctgcagca ggccctcgac ttgaaccccg
     1441 ctcatttctt tctgtggctg cagttcggcc aatgccagca agccctcggg ttgatcggtc
     1501 ccgcccgaat ctccttcgcc caagctcggc agctcaatcc caagtgccag gaagccgcct
     1561 tcgcgctgac cagactgtct gattctggtc tgggatctcg cctgcgcggc tggtggcggc
     1621 gactgttcac ccgatgatcc gctacccata agcagctacc aaccacgaat cacaaatcgc
     1681 caacccccca gcaccaattg atgagctctg agaacctcga ccgcctcctc tccgccgccg
     1741 ccgaattcga tgcctcggac cttcacctgg tcgtcggggt gccgcccgcc tttcgcgtca
     1801 acggcgagat catcatcgca gacgaggacg cgctgacgga agcggaggtc acgcaaattg
     1861 ccgaccatct gctcaatgaa cagcagaaaa agaaatttga gcaggaatgg gagttgtgta
     1921 tttctctgct gcaccccgcc gccgggcgtg tccgggcgac gttctaccgg cgcaacgggc
     1981 atccggaaat gagtttccgc ttctgcggcg atcggatcgc gacgcgggag gaccttgggc
     2041 tgccggagaa gattgatgat ctgtcgcgca agcccaacgg gctggtcctg atcaccggcc
     2101 ccacgggcgc gggcaagacg acgacgctta attacatggt ggacctgatt aatagcgagc
     2161 gccggtgcaa gatcgttacc atcgaagacc ccatcgagtt cgtgcatgcg aacaagcgcg
     2221 cgatcgtcgt gcagcaggaa gtgctcagcg atgtgcgctc ctttaaccgg gcgctgatcc
     2281 atgtcttgcg acaggacccg gacgtgatcg tggtcggcga gatgcgtgac ttcgaggcga
     2341 tctccacggc gcttaccgcc gccgagaccg gacacctggt gctggcgacc atgcactcgc
     2401 ccaatgtttc ccatgcgctg gagcggatcg tgggcgtgtt cgagggcagt gcgcagcggc
     2461 agattattct tcagttgtcc aacgcgttgc agggaattat cgcgcaggag ctgttgcccg
     2521 ccgccgatcg cacccgccgt gtgctcgctt acgaattgat cgtggctaac ggcgccgtgc
     2581 ggaacctgat ccgggagaac caggtccacc agttggagaa caccatccag accgggcgca
     2641 aagagggcat gaccctcatg gacaattgcc tctacgacct gtactgcaag tgccttatca
     2701 cctacgacac ggcggtgagc cgcgcccggc atcccgatcg catcatcccg cagaagacct
     2761 gatcaggggc aagcaactca gatagtgggg caggcgtctc gcctgccgct tgggtcgtct
     2821 gttggggccg ggggcgagat gttctccaga caggcgggac gccttcccca ctacttggac
     2881 agttgccggg acaattgcgg gcgatttgtg cgtgcggatt ggcctccggg tctgtcaaat
     2941 catggcaatg acgcaccata cctaccagat cgtgcgcgga gctgcgctgc tggcgattgc
     3001 tctcagcttc atcggctggg tcatggtgcg ctggctcaag cgcactgacg acccggcgcg
     3061 gctgatcttc aagtggatcc tgaccgtgcc agtcgtcgtg cttctgattt tctgggtggg
     3121 gccgttcgcg acctcgtcgg agggtggcgc cttcattgga gtgccgttag cggcggcgtg
     3181 tgggatagtg ttggccatta tctggcggca caacattgcc gacctgatcg ccaagccgtt
     3241 cgcctcgctc tacgatggcg gcagccagga accgatcccg cacccggcct attcggtcgc
     3301 ccaatcgaaa cagaagcggg gcaattacct ggaggccatt gccgaaatcc gcaagcagct
     3361 cgaccggttt cccaccgacg tggaaggaca actgctgctg gcgcagattc aggccgaaga
     3421 cctcaaagac ctgcccgctg cagagctcac cctcgagcac ttctgcgccc agccgggcca
     3481 tgcgccccag aacatcgcct tcgcgctcta ctcgctagcg gattggcacc tgaaagtcgg
     3541 gcaggatcgc gaggcagcgc ggcgcgacct ggaaaagatt atcgagttgt tccccggatc
     3601 agagttcgcc ctgggagccg cgcaccgcat cgcccacctg ggcagcctgg acatgctgct
     3661 gtcgccgcac gagcgcaaga tattcgctgt gcccgaaggt gtgcaaaacc ttggcctgct
     3721 tcgcgaccag gaccacttga agccggcgga gtcggacccg gaacaggtcg ccgccgagtg
     3781 cgtgaagcat ctggagcagc atccgctgga cacggaggcg cgggagaggc tggcggtcat
     3841 ctacgccgat cactacgggc ggctcgatct ggcggctggc gagctggagc aaatgatcga
     3901 gcagccgaac cagccgggcc ggctggtggt tcattggctc aacgtgttgg ccgatttgca
     3961 gatccggagc ggagccgact gtgagacagt ccggcacacg ctggaacgca ttgttgatcg
     4021 caacccggat gtcgctgccg ccgagattgc ccgccatcgg cttgccttgc tgaagctgca
     4081 gatgaaacca aaagaaaagg gccaagcggt caagcttggt tcttacgaac agaatatcgg
     4141 cctgaagcga ggctcacccc gccgctagct ttatggcgat ctgcttctgc agttcttgcg
     4201 tgagggtgag gtcagtgccg ccgccactag tgcgcacctg caccgtcacg gatgtgaccg
     4261 tgggatccac ggcctggacg ctcacccaaa cacttctctc gctgaccttg ccttcgatgg
     4321 ccctgacctg gccggggccg gggttgatgg tggtttcccg cgtgatggtt ccgttgaact
     4381 tgatcacctc cacggcggcc gcataaacct gctccggcgt ccgctggtag cggccctcga
     4441 acttgtcttt gccgggcgac aacgcaaacg catgacggtc gttcacggtg ctgacgcagc
     4501 ccgccacgag cacaagactc cccaatagaa ctaaaagcat ctttatcttc atctctgcta
     4561 tgcaaccaca ttcccgccaa tcgtcaagtc cggattcaag gttggacttc ctggagcgcc
     4621 cgccaactgg cagccacgcg gccgagggtg gcgcgcacgc tcatctgtga cgcggtgcgc
     4681 gggttggagg ggatggcgag cgtgcggctg atctggccga atgggct
//
LOCUS       DATCGS010000375         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_279105, whole genome shotgun sequence.
ACCESSION   DATCGS010000375 DATCGS010000000
VERSION     DATCGS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279105"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1528
                     /locus_tag="VIL39_07745"
     CDS             113..1528
                     /locus_tag="VIL39_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEY5481356.1"
                     /translation="MNRRDFIRNTSLLAASTMLGTVSGAAESGFPVVRIPEAKRKFKS
                     RAVERTIETIRSRIGNKELAWMFENCFPNTLDTTVDFEVVDGRPDTYVITGDIDAMWL
                     RDSTAQVWPYLPLMKNDSDLQQLIAGVINRQTRCILKDPYANAFYKDEKKVSEWKSDR
                     TDMKPGVHERKWEIDSLCYPIRLAYNYWKATNDRAPLDASWREAILRTLQTFREQQRK
                     AGQGPYRFQRRTEVQTDTLAGGGYGRPAKPVGLICSMFRPSDDSTVFPYLVPSNFFAV
                     VSLRQAAELLQQLHHDQESATQCRALADEVERALREYAVVIHEKFGRIFPYEIDGYGD
                     YYCLDDGNVPSLLSLPYLDVVGPGDPLYLNTRRFILSDSNPYYCIGKAAQGPGGPHVG
                     RDMIWPLGLIVQGLTSTDDQEVRQCLLTLQKTHAGTGFMHEAFHKDDPDKFTRKWFAW
                     ANTIFGELVLKSYKERPHVLS"
     gene            complement(1640..1870)
                     /locus_tag="VIL39_07750"
     CDS             complement(1640..1870)
                     /locus_tag="VIL39_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481357.1"
                     /translation="MIHEHTQPRPMPPQALQNLLLFRPQLAAGQLAALLPAPTQRPKC
                     NGDIGGLITTNGGRLIADRPPLHHRTILSQQT"
     gene            1820..2197
                     /locus_tag="VIL39_07755"
     CDS             1820..2197
                     /locus_tag="VIL39_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481358.1"
                     /translation="MKSLRRHWTGLCVFVDHPQVPMDNNTAERTQPRGDRHEPSAALA
                     LLRTRLQRGGPGPHPGAIAPPAAPALRTAQTDRENRAQPCSHPSPASSRTLQPVPKPS
                     PARLPPSRCHPSPVMQHPALAAK"
     gene            2338..2757
                     /locus_tag="VIL39_07760"
     CDS             2338..2757
                     /locus_tag="VIL39_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481359.1"
                     /translation="MGRSYWFECPKCGYRAKVSGREDRGLIFFVQTILCLDCRELYDA
                     VTRLRVPDDRIGLGDLSRWHRARLKPQRSFSAPPAFQAVLNWLPQTGVKRLKWLPFKL
                     QCPVSVMHRVRSWNDPDKCPRCGVYLEKSALPYRLWD"
     gene            complement(2802..3380)
                     /locus_tag="VIL39_07765"
     CDS             complement(2802..3380)
                     /locus_tag="VIL39_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5481360.1"
                     /translation="MHNVVTNYVHFMWAALLKLRQTGAIIPSQRFLIEKMIAPVPVAY
                     SGQIIELGAGNGALTLRLAARCPEARILACEINPALARVNRHNLDMAGINGRVQVIPD
                     SAEHLLSEMDRRGMGKADFVLSGIPLGNLPGDRVLALIDAVNQALVPGGMYIQFQYSL
                     LDRKRIRTRFQTLHTAPAFLNIPPAVVYYAQK"
BASE COUNT          713 a         1110 c         1126 g          671 t
ORIGIN      
        1 cgtcgggggt ggtatccgca agatgctccc ggcggtaaga gcaaggccag aattagctgg
       61 acgcaatcca gcgcgacttt aatctgactg caaccagcag tttattaaac ctgtgaaccg
      121 aagagacttc attcgcaaca cctcgttgct cgccgccagc acgatgctcg gcacggtgag
      181 cggggcggcc gagagcggct tcccggtcgt ccgcatcccc gaagccaagc gcaaattcaa
      241 gagccgcgcc gtggagcgca ccatcgaaac catcaggagc cggatcggca acaaggagct
      301 ggcctggatg ttcgagaact gcttcccgaa caccctggac acgaccgtgg atttcgaggt
      361 ggtggacggc cggcccgaca cctacgtgat cacgggagac attgacgcga tgtggctgcg
      421 ggacagcacg gcgcaggtct ggccctacct cccgctgatg aagaacgatt ccgaccttca
      481 gcaattgatc gccggggtga tcaaccgcca gacgcgctgc atcctgaagg acccttacgc
      541 caacgccttc tacaaagacg agaagaaggt gagcgagtgg aagagcgacc gcacggacat
      601 gaagcccggc gtccacgaac gcaagtggga gatcgattcc ctctgctacc cgatccgcct
      661 ggcttacaac tactggaagg cgaccaacga ccgcgcgccc ctcgatgcct catggcggga
      721 ggcgatcttg cgcacgctcc agacgttccg ggaacagcag cgcaaggccg ggcagggacc
      781 ttaccgcttc caacgccgca ccgaggtcca aacggacacc ctcgccggcg gcggttatgg
      841 ccggccggcc aaaccggtgg gcctgatctg ctccatgttc cgccccagcg acgactcgac
      901 cgttttcccg tacctggtcc cgtcgaattt cttcgcggtc gtcagcctgc gccaggctgc
      961 ggaactgttg cagcaactcc atcatgatca ggaatctgcg acccaatgcc gtgcgctggc
     1021 cgacgaggtc gagcgcgctt tgcgcgaata cgctgtcgtg atccacgaga agttcgggcg
     1081 cattttccct tacgagatag acggctacgg cgattactac tgccttgacg atggcaacgt
     1141 ccccagcctg ctttccctgc cctatctcga tgtggttggg ccgggggacc cgctgtatct
     1201 caacacgcgc cggttcatct tgtccgacag caacccctat tattgcatcg ggaaggccgc
     1261 ccagggcccg ggcggaccgc acgtgggccg ggacatgatc tggccgctgg ggctgatcgt
     1321 gcaagggctg accagcacgg atgaccagga agtccgccag tgcctgctta ccttgcagaa
     1381 gacccatgcc ggcaccgggt tcatgcacga ggcatttcac aaggacgacc cggacaagtt
     1441 cacgcgcaag tggtttgcct gggccaacac gatcttcggc gagctggtcc ttaagagcta
     1501 caaagagcgg cctcatgtgc taagttagcc tgcgggtaac tgtaaccttt gcccggctca
     1561 ggctgtctga tgtaggggag gctgcggggc ggtttgtgcg ccacgacagc ttggccggga
     1621 ggaatcgagt atggcagacc tatgtttgtt ggctgaggat ggtgcggtgg tgcaacggtg
     1681 ggcgatcggc gatcaggcgg ccgccgttgg tcgtgatgag accgccgata tcaccattac
     1741 acttgggacg ctgggttggc gcgggcagca gggcggcgag ttggcccgcg gcgagctgcg
     1801 gccggaacag gagaagattt tgaagagcct gcggcggcat tggacggggc tgtgtgtgtt
     1861 cgtggatcat ccgcaggtgc cgatggataa caacacggcc gagcggaccc agcccagggg
     1921 cgaccgccat gaacccagcg ccgcgctggc gttactgcgg acgcgacttc agcgcggcgg
     1981 acctggccca catccaggag ctattgcgcc gccagccgcg ccggccctcc gcaccgcgca
     2041 gaccgatcgg gaaaaccgcg ctcaaccctg cagccacccc tctccagcca gttccagaac
     2101 cctccaaccg gttcccaaac cttcacccgc ccggctgccg ccatctcgct gccacccatc
     2161 accagttatg cagcaccccg ccttggcggc aaaatgacca aagcgagaac tgctgtctgg
     2221 ttgtggatcc tggttgccaa aatgcgagtg gctgctagac ttgccgttga ccgtgtgtat
     2281 ttgggcgcac cctgtttggt gcgccttttc ttagtacatc acagacgagg aattcgtatg
     2341 ggccggtcgt attggtttga gtgtcccaag tgcggttacc gggctaaggt ctccggtcgc
     2401 gaggaccggg ggctgatctt cttcgtccag accatactct gtctcgattg cagggagctg
     2461 tacgatgccg tcacgcgtct gagagtaccg gacgaccgga tcgggctggg agacctttcc
     2521 cgctggcatc gggccaggtt gaagccgcaa cggagcttca gcgcgccgcc cgcttttcag
     2581 gccgttctga actggttgcc ccaaacggga gtgaagcggc tcaagtggct tcctttcaag
     2641 ctccagtgcc cggtttcggt gatgcaccgg gttcggagct ggaacgatcc ggacaaatgc
     2701 ccgaggtgcg gcgtttacct ggagaagagc gcgctgccgt atcggctctg ggattgaggg
     2761 gcaaccactg agccgcgccc cggaccatgg gcgacggctg gttacttctg cgcgtaatac
     2821 acgaccgcgg gaggaatgtt cagaaatgcg ggagcggtgt ggagggtttg gaaccgcgtc
     2881 cgtatccgct tgcggtccaa cagcgagtac tggaattgaa tgtacattcc gcccggaacc
     2941 agcgcctggt tgacggcgtc aatgagggcg agcacccgat cgccggggag attgcccaga
     3001 ggaatgcctg agaggacgaa atccgctttg cccatgcctc tgcgatccat ttccgaaagc
     3061 aggtgttcgg cggaatcggg aatcacttgc acccgaccgt tgatgccagc catgtccagg
     3121 ttgtgccggt tgacgcgcgc cagcgccgga ttgatctcgc aagccaggat acgggcctcc
     3181 ggacaccggg cggccagccg taaagtgagc gccccgttgc cggcgcccaa ctcgatgatc
     3241 tgtccggaat aggccaccgg cacgggcgca atcatcttct caatcaggaa cctctgggag
     3301 ggtataatgg cgcccgtttg cctgagcttc agcaaggcgg cccacatgaa atgcacgtaa
     3361 tttgtaacca cgttatgcat agcgattccg ttgtaatcaa aacttggctc gggcacggtg
     3421 cccaaaggac accctgctcc gaaatggcct attcttccaa ccccagacaa aggcaggccc
     3481 tcgcgagccc ctgtctcccg gttcgcctgt tggggcaata acgcaacaat gttagcatgc
     3541 gccccggcag gttccaacgc ctaatttgag gtaatttgcg gcgatgcggg ggcttggcgg
     3601 gtacaacctc ccgcagctca
//
LOCUS       DATCGS010000376         3780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_576712, whole genome shotgun sequence.
ACCESSION   DATCGS010000376 DATCGS010000000
VERSION     DATCGS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576712"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..1003
                     /locus_tag="VIL39_07770"
     CDS             551..1003
                     /locus_tag="VIL39_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY5481361.1"
                     /translation="MTIKNAPDLTQCPPRSSRVRLGGYVILPRMLDKGRATLAGKHGE
                     YNYACPLDHRFLDFVGVKPDALKKQLAAGKGDAEILEWIEKNAKHTYTEPEIHAWSAY
                     QEQRAPADTESREFFHEAHKKIAPKREDIATWFDLLDVDDYVSFGGKA"
     gene            complement(1077..2897)
                     /locus_tag="VIL39_07775"
     CDS             complement(1077..2897)
                     /locus_tag="VIL39_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY5481362.1"
                     /translation="MKEEFEKMAAAGKLERHHIEALVRLTDTGFCMHRSWGFGRIKTM
                     DTVFARFMIDFDNKPGHTMDLVFAAESLKPIAKDHILARKALDVEALRQMAATQHLEL
                     VKLVLNSYGGKATVEQLQQVLVPDVIREDWKKWWDAARRELKKDGHFQVPLKKTEPII
                     YQTKEVSLQDRLMGEFRAAKGLKARIAVASELLRNAHDLTDKGAAATEIIATFNTEIP
                     SYQRTQPAVALEAIFARDDVRQMADLPLGEGEITAPLIWAQNAKLGPLLEQLPASKHR
                     RGLESFKAANPEHWQAALQVALNTVSAKVCRELVSLLIQEGKIEQLKEAVARLVSQHS
                     ASSELLLWLAKDRNDSFADILGPEVFRAMLTAMERDQFSERRSNRLRDFILDDQQLLV
                     ELIGSADLEVIKDLTRGLQLSPSFDDMDKRSLLARIVKSYPTIQVLISGEQTKQEATL
                     LVSWESLERRKNEYRELVEKKIPANSKEIAVARSYGDLSENHEYKAAKEMQKLLMTRK
                     ADLETQLTRARGTDFAAPRTDVASIGTIVQATNLATNQPEHFTILGAWDSDPEKDVIS
                     YLSPVAQSLLGHKVGDEVEVEVHGVCHHHRIGSIQAYKTA"
     gene            2949..>3780
                     /locus_tag="VIL39_07780"
     CDS             2949..>3780
                     /locus_tag="VIL39_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5481363.1"
                     /translation="MRDEKPREIALRILQRDAGGEFVEDRFEAELARTRLSPPDRHLC
                     QELAYGIVRWQATLDWLIARKTDARPQKPMLQNLLRLGLYQIFWLNRIPDHAAVNETV
                     ELARQSGFHSQAGFINAVLRGYLRESDATRRLLAELKTAQPHLGYSHPEWLVAHWQTR
                     WGADHAAQLMEWNNTPPKAFARINTLKADPAKLLVQWRDENVEYDFVRRDWAEENLVF
                     ELKAHPPLGQMPSFQQGLFYVQDPSTLLAVRELDPQPGETVLDLCAAPGGKLTFIAQL
                     M"
BASE COUNT          719 a         1199 c         1113 g          749 t
ORIGIN      
        1 cgcgacgccg ctttccctga cagcagtaat ccgctgatgc agcagaagtg ccacccgcag
       61 ccaaagcggc gtcgcgcttc gcttgccgcc gcactccaca tccgtgatgc cgggacatcc
      121 cggcaacttt gtcaaccgcc atggccctgc gtgggaagga agccaccggt caaagcggat
      181 gagccgacga acagttgtga gccgcaacga gccgggggcc ggagaacggc ccgtgcaaac
      241 gaagagaccg gagggaccga ttccaacaac gtgcgaagac aggtgatctc cggtgcccca
      301 acgggacagc cctatatcag cccagcaagg cccgccggag cctagctaga acgtcagaac
      361 tcagcggcgg gcagaggacc gccccgcctg cccggtgcga gcggcttccg cgagcttgat
      421 gcagccctgt tcccacgcgc tgcccctcac ttgcatttct cccgattggc tgcatcttag
      481 ccgtgaccaa agcgggtccg acccggtgct gaactcgcga ggtgtttacg aattaataac
      541 aaaatgttct atgactatca aaaatgctcc tgatctaact caatgtccgc cgcgcagttc
      601 gcgggtgcgg ctcggcggtt acgtcattct gccgcgcatg ctcgataagg gccgcgcgac
      661 gctcgccggc aaacacgggg aatacaatta cgcctgcccc ctcgaccacc gcttcctgga
      721 cttcgtcggc gtcaagcctg acgcgctcaa gaaacaactt gcagccggaa agggcgatgc
      781 cgaaatcctc gaatggattg agaagaacgc gaagcatacc tataccgaac cggagatcca
      841 cgcgtggtcg gcttaccagg agcagcgggc gcccgcggac accgaatcac gcgagttttt
      901 ccatgaggcg cacaagaaga tcgcgcccaa acgcgaggat atcgccacct ggttcgacct
      961 gctcgacgtg gatgattacg tctcgttcgg cggcaaagcg taaagcctcg cgccggccgt
     1021 tcgatggccg gtcccctggt actgcttcag ggccggaggt ttgagggcgt gcggccctac
     1081 gccgtcttgt aggcctggat cgagccgatg cgatggtggt ggcagactcc gtgaacctcg
     1141 acctcaacct catccccgac cttgtggcca agcagtgatt gggcgaccgg tgaaaggtag
     1201 ctgatgacat ccttctccgg gtccgagtcc cacgcgccaa gaatggtgaa gtgctccggc
     1261 tggttcgtgg ccaggttggt tgcctgcacg atggtgccga tgcttgcgac gtcggtgcgc
     1321 ggcgccgcaa aatcagtgcc gcgcgcgcgt gtgagctggg tttccaggtc cgccttgcgc
     1381 gtcatcagca gcttctgcat ttccttggcc gctttgtatt cgtggttttc gctcaggtcg
     1441 ccgtagctgc gggccaccgc gatttccttc gagttggcgg ggatcttctt ctccaccagc
     1501 tcgcggtatt cgttcttgcg ccgctccagg ctctcccagg acaccagcag ggtggcctcc
     1561 tgtttggttt gctcgcccga gatcagcacc tggatcgtcg ggtagctctt cacgatgcgc
     1621 gccagcagcg agcgcttgtc catgtcatcg aacgacggcg agagctgcaa tccgcgcgtc
     1681 aggtctttga tgacctccaa gtcggcggag ccgatgagct caacgagcag ttgctggtcg
     1741 tcgagaataa agtcgcgcaa ccggttggag cggcgctcgc tgaactggtc gcgctccatg
     1801 gcggtgagca tcgcgcgaaa gacttccggt ccgaggatgt ccgcgaagga gtcgttccgg
     1861 tctttggcga gccagagcag gagttcgctg ctggcagagt gctggctaac gagccgcgcc
     1921 actgcttcct tgagctgctc gatcttgcct tcctgaatga gcaggctgac cagctcgcgg
     1981 cacaccttgg cggaaacggt gttgagcgcc acctgcagcg cagcttgcca gtgctcagga
     2041 ttcgcagcct tgaatgactc cagcccccgg cgatgcttgg atgccggaag ctgctccagc
     2101 agcggcccca gcttcgcatt ctgcgcccaa atcaacggtg ccgtgatttc gccttcgcca
     2161 agcggcaagt ccgccatctg ccgaacgtca tcacgggcaa agatggcttc gagcgccact
     2221 gccggctgcg tgcgctggta gctggggatt tcggtgttga acgtggcgat gatctccgtg
     2281 gcggcagccc ctttgtccgt gaggtcgtgc gcattccgga gcagttcgct ggcgacggca
     2341 atgcgggcct tcaatccttt ggccgcccgg aattccccca tcagccggtc ctgcagtgaa
     2401 acctctttgg tctgatagat gatcggctcg gtcttcttga ggggcacttg aaaatggccg
     2461 tccttcttca gctcgcgccg ggcggcgtcc caccatttct tccaatcctc gcgaatgaca
     2521 tcaggtacga gcacttgctg gagctgttct accgtcgctt tgccgccgta gctgttcaat
     2581 accagcttaa ccagctccaa gtgttgcgtc gccgccatct gacgcagcgc ttccacgtct
     2641 aaggccttgc gcgcgaggat gtggtctttg gcaatcggct tgagcgactc ggcggcaaaa
     2701 accagatcca tcgtgtgccc gggcttgttg tcgaagtcga tcatgaagcg ggcaaatacc
     2761 gtatccatcg tcttgatgcg tccaaaaccc cagctccggt gcatgcagaa accggtatcg
     2821 gtcagtcgca ccaaggcctc aatatggtgt cgctccagtt tccccgccgc cgccatcttc
     2881 tcgaattctt ctttcataaa gtatcttaac gctcgcatct cccgagcaga acggctctat
     2941 taaaggttgt gcgcgacgaa aaaccaagag aaattgcact gcgcatcctg cagcgggatg
     3001 cgggcgggga gtttgtggaa gaccggttcg aggcggaact ggcccgaaca aggctttcgc
     3061 cccctgatcg ccatctgtgc caggagctgg cctatggcat cgtccgctgg caggccaccc
     3121 tcgactggct gattgcccgc aagacagacg cacggcccca gaagcccatg ctgcagaacc
     3181 tcttgcggct cggcctttac cagatcttct ggctcaaccg catccccgac catgccgcgg
     3241 tcaacgagac ggtcgagcta gccaggcaaa gcggctttca ctcgcaggcg ggattcatca
     3301 atgccgtcct gcgcggttat ctgcgcgagt ccgacgccac caggcgcctg ttggccgagc
     3361 tgaaaacggc gcagcctcac ctcggctact cccatccgga atggctggtg gcgcactggc
     3421 aaacacggtg gggcgccgac catgcggcgc agttgatgga atggaataac accccgccaa
     3481 aagcgttcgc ccgcattaac actttgaagg ccgaccccgc taaacttctg gtccagtggc
     3541 gcgacgaaaa tgtggaatat gacttcgtcc gccgggactg ggccgaagag aacctggtct
     3601 tcgagctcaa agcgcatccg cccctgggcc agatgcctag ttttcaacaa ggtttattct
     3661 acgttcagga ccccagcacc ctgctggcgg tgcgcgaact cgatccacag cccggcgaaa
     3721 ccgtgctcga cctctgcgcc gcccccggcg gaaaactcac cttcatcgcc caattgatgc
//
LOCUS       DATCGS010000377         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_626212, whole genome shotgun sequence.
ACCESSION   DATCGS010000377 DATCGS010000000
VERSION     DATCGS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626212"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..>3045)
                     /gene="glnE"
                     /locus_tag="VIL39_07785"
     CDS             complement(82..>3045)
                     /gene="glnE"
                     /locus_tag="VIL39_07785"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY5481364.1"
                     /translation="LGMKNPVWTNAIKSCADPQRAKHFLDLLAATDAGAALQASSAEQ
                     ARILAALFSGSQALSNLLVARPDWLGALEPEVLKFPRRKQGLRNEVSRWLKPLLAASD
                     FGAALTRVREFKQREMLGIAGRDLARLGKLPETIQEISDVADVCLEAVWQVCYRQLVG
                     RYGQPWHQDAGGQWQPTAGCVFGMGKLGGQELNYSSDVDVIFAYSEEGSVFKEPPGKS
                     KAPQPVLTNHQFFNRLAETFIAEVSRMTLEGALYRIDLRLRPEGDAGPLSRSLAGYEN
                     YYAQWGQTWERMMLIKARGVAGDAALAAEFLELIQPFRYPRSINESVLSEIVAMKGRI
                     ETEVVKAGELERNVKLGRGGIREIEFVVQALQLLRAGRQPFLQGAQTLPSLAKLVQYE
                     LVTPDESRMLTEAYCFLRDVEHRLQMEDNLQTHTIPTERRAQERLARLMGFATLAEFE
                     TSRQAHTHNVRRIFDRLLKADACEGEAPSPFPREFEGAEPEWKKLLAEHSFKDADKAF
                     RVLREFVEGPGYVHVSPRTSQLAYQLLPKLFALCPQPRSGAPSPDSHRPPQTANVPLS
                     DPDRVVTRLDSFISAYGARATLFELWNRNPTIFELLVLLFDRSEFLAELAIRTPDLVD
                     ELVSSGRLRQRKSAEETLRDLRHGLADEDQRLWLRRYHQAELMRIGLRDILGLADFEQ
                     YLTELSGLADACLQYALEVVLRQHKVKSLPFVVIGLGKLGGAEIDYGSDLDVVFVADN
                     KAKNLPALGRLALEVLDLLSARTEQGVVFRTDARLRPDGEKGLLVNTLRAYEDYYRQR
                     GQLWEIQSLTRTRPVAGDLKVGAQFQTLAGTLTNFRHPALPLAGYGPDWKQKIHQMRM
                     RIEKERTPLGKDDLAIKTGNGGLMDAEFIAQALCLDNGWQEANTLRALERGRAARVLP
                     EADKLIENYRQLRRLEGILRRWSYEGETVLPDDPAPYYRVSIRCGFSTPGAFREALAG
                     WRRAIREVYLTVFQA"
BASE COUNT          545 a          984 c          948 g          568 t
ORIGIN      
        1 gggccctggc ggcgggctgt ttctttagac tctggttcca tctggcaatt cctcagtcaa
       61 ccgcctcctg ggatcgccgg ctcatgcctg aaacaccgtc aaatacacct cccgaattgc
      121 gcggcgccac cccgccagcg cttcacgaaa cgcgccgggc gtgctaaagc cgcaacggat
      181 cgagacgcgg taatagggtg ctggatcatc gggcaagacg gtttcacctt cgtagctcca
      241 gcgccgcaag atgccttcga ggcgccgaag ctggcggtag ttctcgatga gcttgtccgc
      301 ttctggcagc acgcgggcag cgcggccgcg ttccagggcg cgcagggtgt tggcctcctg
      361 ccagccattg tccaggcaga gtgcctgcgc gatgaactcc gcgtccatca gtccgccgtt
      421 gccggttttg atagcgaggt catcctttcc caatggggta cgttcctttt cgatgcgcat
      481 ccgcatctgg tgaatcttct gcttccaatc cggtccatag ccagccagtg gcaacgccgg
      541 gtgccggaag ttcgtcagcg tgccggccag ggtttgaaac tgggcgccca ctttcaggtc
      601 gcccgcgacc ggacgggtgc gcgtgagcga ttgaatctcc caaagctgtc cgcgctgccg
      661 atagtaatct tcgtaagcgc gcagtgtatt gaccaggagg cctttctcgc cgtcaggccg
      721 gagccgagcg tcggtgcgaa agacgacgcc ttgttcggtg cgcgccgaga gcaggtcgag
      781 cacttcgagg gcgaggcggc ccaacgcggg cagattcttc gccttgttgt ccgcgacgaa
      841 gacgacatca aggtccgacc cataatcaat ttccgcgccc cccagcttgc cgaggccaat
      901 gacgacgaag ggcagggatt tgaccttgtg ctggcgcagc acgacttcga gggcgtattg
      961 caggcaggcg tccgccaatc cggacagctc ggtgagatat tgctcgaagt ccgccaggcc
     1021 caggatgtcg cgcaggccga tgcgcatgag ctcggcctgg tgataacggc gcagccacaa
     1081 ccgctggtct tcgtcagcaa ggccatggcg caaatcccgc aaggtttcct cggccgactt
     1141 gcgctggcgc aggcggccgc tgctgaccag ctcatccacc aggtccggcg tgcgaatggc
     1201 cagttccgcc aggaattccg agcggtcaaa caagagcacc agcaactcaa agatcgtggg
     1261 gttgcggttc cacagttcaa agagcgttgc ccgcgcgcca taggcactaa tgaagctgtc
     1321 gagacgtgtg acaacgcggt cgggatcgga aagcggcacg ttagccgtct gcggcggcct
     1381 gtgggaatcg gggcttggag cgcccgacct tggctgggga catagcgcga agagcttcgg
     1441 caacaactga tacgccagtt ggctggttcg cggcgaaaca tgaacatagc ccggcccctc
     1501 gacgaactcg cgcagcacgc ggaacgcttt gtccgcgtcc ttgaaggaat gttcggccag
     1561 caacttcttc cattccggct cggcaccctc gaactcgcgc gggaacggcg acggagcttc
     1621 accttcgcat gcatctgcct tcagcaaccg gtcgaagatc ctgcggacgt tgtgggtgtg
     1681 ggcctggcgc gaggtctcga actcggcgag ggtcgcgaag cccatcagac gagccaggcg
     1741 ttcctgggcg cgccgctcgg tcgggatggt gtgcgtctgc aggttgtcct ccatttgcag
     1801 gcggtgttcg acgtcgcgca ggaagcaata cgcctcggtg agcatcctcg attcgtccgg
     1861 cgtgaccagc tcgtattgga ctagtttagc cagcgaggga agggtctgcg cgccttggag
     1921 aaacggttgc cggccggcgc gaagcagttg tagcgcttgc accacgaatt cgatttcccg
     1981 aatgccccct cgccccagct ttacgttgcg ctccaattcg ccggccttca ccacctcggt
     2041 ctcgatgcgg cctttcatgg ccacgatctc actcagcacg ctttcgttga tggaccgcgg
     2101 ataccggaac ggctggatca gctccaggaa ttccgcggcc agcgccgcgt cgcccgccac
     2161 gccgcgggct ttgatcaaca tcatccgctc ccaggtctgg ccccattgcg cgtagtaatt
     2221 ctcgtagccg gccagggacc ggctgagcgg acccgcatcc ccttcgggcc gcagccgcaa
     2281 atcaatccgg taaagggcgc cttccaaggt catgcggctc acttcggcga tgaaggtctc
     2341 agccaggcga ttgaagaact ggtggttcgt cagcaccggc tgcggcgctt tgcttttccc
     2401 cggaggctcc ttgaagacgc ttccctcctc gctataggcg aagatcacgt ccacatctga
     2461 gctgtaattc agttcctgtc cgccgagctt gcccatgcca aacacgcagc cggcagtcgg
     2521 ctgccactgc ccgccggcat cctggtgcca gggttgaccg taacgcccga cgagctgccg
     2581 gtagcagact tgccagacgg cctcgaggca gacatcggcc acgtcggaaa tctcctggat
     2641 ggtctccgga agcttgccca gccgcgcgag gtcgcgcccg gcgatgccaa gcatctcgcg
     2701 ctgcttgaac tcccgcacgc gcgtaagggc cgcgccgaag tcggacgcgg ccagcagcgg
     2761 cttgagccac cggctcacct cattgcgcag accctgtttg cgccggggaa acttgagcac
     2821 ttccggctcc agcgcgccga gccaatcagg ccgcgccacg agcagattgc tcagcgcctg
     2881 ggaaccactg aagagcgcgg cgagaatgcg ggcttgctcg gccgatgacg cctgcaaagc
     2941 cgcgccggcg tcagtagccg cgagcaagtc aaggaagtgc ttggcccgct gcgggtccgc
     3001 gcagctcttg atggcattcg tccagaccgg attcttcatt cccaa
//
LOCUS       DATCGS010000378         3985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_181630, whole genome shotgun sequence.
ACCESSION   DATCGS010000378 DATCGS010000000
VERSION     DATCGS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181630"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..2615
                     /locus_tag="VIL39_07790"
     CDS             294..2615
                     /locus_tag="VIL39_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481365.1"
                     /translation="MTSFSSLRYRLVGTVLLAIAPAGIIMYFVDKYYLAKNDAHLPWA
                     PIVIGLVALGAAWFGGERFILRQVRILSKAAQQFAVGDLTRRTGLSRERGELGELAQT
                     FDGMAESLDQRIKERERAEKALLNRSFQQTVVAALGQFAIVSNDISALLNQVVMLVAQ
                     TLELEYCHVLRLQPGDKSLRLHAGVGWKSGYVGKAVLPADPQTEAGFTLGAGEAVAFE
                     HLPSETRFRGSALLTDHGVVSGISVAITGHGQLFGTLGAYTAHQRRFTEDEIHFLFSI
                     ATVLAMAVERIRAETELQNLAAFVQLNPNPAMELAGDGTVNYFNDAFLKLGCLASEIL
                     PPNVQEIVHHCLELGQSPPKLETVFKGRTLSWSFHPVAPSQVVHCYVEDVTTRLNLEA
                     QLRQSQKMESIGQLAAGVAHDFNNMLTVIQGHSGMILARSNLPPELLNSAQAIYFASE
                     RAAGLTRQLLMFSRKNVMKSEPRDLRKVVGDLSKMLKRLLGETVTLEFSPSPALPLVQ
                     ADTGMVEQVIMNLAVNARDAMPNGGTLTISTNPVEISEDYVQTHSEARLGAFVCLRVS
                     DTGTGMDSATMTRIFEPFFTTKDVGKGTGLGLATVYGIVKQHEGWIEVSSEVGKGSTF
                     SVFFPASSEPITALPRESPFAAPVRGGHETILVVEDEPVLREMAHLILQDCGYNVLEA
                     GSGAEALQVWECNGDRIDLMLTDVVMPGGMSGRDLAAKLMANHPQLRIIFTSGYNVEE
                     TSTDFFRRGGAAFLQKPYTRIRLAEVVRQCLDK"
     gene            complement(2642..3133)
                     /locus_tag="VIL39_07795"
     CDS             complement(2642..3133)
                     /locus_tag="VIL39_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEY5481366.1"
                     /translation="METKLKVIQIEKPEAVNFILGQTHFIKSVEDLHEALVGAVPGIK
                     FGLAFCEASGKCLVRWTGTDPAMIELARKNALAIGAGHSFILFLGEGFYPVNVLNAVK
                     MVPEVCRVFCATANPTQVLIAETTQGRGIVGVVDGCPPRGVEGDADIEWRRKFLRQIG
                     YKL"
     gene            3274..>3985
                     /locus_tag="VIL39_07800"
     CDS             3274..>3985
                     /locus_tag="VIL39_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5481367.1"
                     /translation="MNEYILDSRQAASAKQFERQSDRYGKSHILANTEDVEAALKGIS
                     VPVGGAALDIATGGGHTALWLARHGWKVTAGDIAPRMLKNAAQLCAEAGLSIETKLCP
                     AEKLPFGAGAFELVTARVAPHHFSSPARFVGEVARVLKPGGRFLLIDGSVPDDDPPTE
                     EWLHHVEKLRDPSHGRYLSRKTWEGLVRDAGLTVERSELQPYKQPDLQWYFETAATPP
                     GNREQVMAAVGGAAKQVRA"
BASE COUNT          777 a         1164 c         1266 g          778 t
ORIGIN      
        1 gcgaccgttg aaaagcccgt gcttttgctg aaccttaagt aagcgccatg ccccctgcgg
       61 ggtagtaggc cagcttgcca aacagccgcg ctaatctgag cgcgcctgac ttgtgggtaa
      121 tgacaagggc tactacccca aggcctgggc gcggaaacca cctgttttcc gcgggctttg
      181 gccataatga gaattgctgc ggtctcccgg tgcgagaacc gtccgcacga atgtttttgc
      241 ctcgggcgca tcagtccgct actgttgccc gacagcaaac gcttgaagac cctatgacca
      301 gtttctccag cttgcggtat cggttggtgg gaacggtctt gctggccatc gccccggcgg
      361 ggataattat gtacttcgtg gacaagtact acctcgctaa gaacgacgct caccttcctt
      421 gggcgccgat cgtgataggg ttggtggcct tgggggccgc gtggttcggc ggagaacgtt
      481 tcatcctccg ccaggtccgc atcctgtcca aagcggccca gcaatttgcc gtgggcgatc
      541 tgacccgccg caccgggtta tcgcgcgagc ggggcgaatt gggggagctg gcccaaacgt
      601 ttgatggtat ggcggagtcg ctcgatcagc gcatcaaaga gcgtgagcgg gcggaaaagg
      661 ccctcctcaa ccgatccttc caacaaaccg tcgtggccgc cctgggccag ttcgccatag
      721 tgagcaacga catctccgcc ctgttgaatc aggtggtcat gctcgtggcg cagacgcttg
      781 agctcgagta ctgccatgtt ttgagattgc agccggggga caagtctctg cggttgcacg
      841 cgggtgtcgg ttggaagagc ggttatgtcg ggaaggcggt ccttccggcg gacccgcaga
      901 ctgaggccgg gttcacgctg ggggccgggg aggcggtggc gttcgagcac ctgccctcgg
      961 agactcgctt ccggggctca gcgctgttga ccgaccacgg tgtcgtgagc ggaatcagcg
     1021 tcgcgattac cggacatggg cagttgtttg gcaccttggg ggcgtacacc gcgcaccagc
     1081 ggagattcac ggaggacgag atccatttcc tattctccat cgcgacggtg ctggcgatgg
     1141 cggtggagcg aattcgtgct gaaaccgaat tgcagaacct ggccgctttc gtccaattga
     1201 atcccaaccc ggctatggaa ctggcgggcg acggcaccgt caactatttc aacgatgcct
     1261 tcctcaagct cggctgcttg gcgagcgaga tcctgcctcc caacgtccag gaaattgtcc
     1321 accactgcct ggagttgggc caaagcccgc cgaagctgga aaccgtattc aaagggcgca
     1381 ccctatcgtg gtcgtttcat ccggtggccc ccagccaggt ggtccattgc tacgtcgaag
     1441 acgttaccac ccggttgaac ctggaagccc agttgcgcca gtcgcagaag atggagtcaa
     1501 tcggccaact ggccgccggc gtcgctcacg atttcaacaa catgctcacg gtgatccagg
     1561 ggcactccgg gatgatactg gccaggagca acctcccgcc cgagttgctc aattccgcgc
     1621 aggcgatcta cttcgcctcg gagcgcgccg ccggtctgac ccgccagttg ctcatgttca
     1681 gccgcaagaa cgtgatgaag tccgagcccc gcgacttgcg taaggtggtc ggcgacctgt
     1741 caaaaatgct caagcggctg ctcggcgaga ctgtcaccct cgaattcagc ccgtcgccgg
     1801 cacttcccct cgtacaggcg gataccggaa tggtcgagca ggtcatcatg aacctcgccg
     1861 tcaacgcccg cgacgccatg ccgaatggag gcacgttgac gatcagcacc aacccggtcg
     1921 agatcagcga ggactacgtc caaacccatt ccgaggcgcg cctgggcgcg ttcgtttgcc
     1981 tgcgcgtcag cgataccgga accggcatgg actcggccac aatgacccgc atattcgagc
     2041 cgttcttcac cactaaagac gttggcaaag gcactggcct tggcctcgcg acggtctatg
     2101 gcatcgtcaa acagcacgag ggctggattg aagtcagcag cgaagtcggc aaaggcagca
     2161 cgtttagcgt attcttcccc gccagtagtg agcccataac agcgctgccg cgggagtccc
     2221 cttttgcggc tccagtgcgc ggcggccacg agactattct ggtggtggag gacgaaccgg
     2281 tgctgcgcga gatggcgcat ctgattctgc aggattgcgg ctataatgtg ttggaagcgg
     2341 ggtctggtgc ggaagccttg caagtctggg aatgcaatgg cgaccgcatt gacctgatgc
     2401 tgacggacgt ggtcatgcct gggggaatgt cgggccggga tttggcggca aaactcatgg
     2461 cgaatcaccc ccagctcagg atcatcttca ccagcggcta taacgtggaa gagacgagca
     2521 ctgacttctt ccgccgaggc ggggcggcct ttttgcagaa gccttacacc cgtattaggc
     2581 ttgccgaagt agtgcgccag tgtttggaca agtaagccgg aacaggaggg gcaaagcgcg
     2641 gttatagttt gtagccgatc tggcggagga acttcctgcg ccattcaatg tccgcatcgc
     2701 cctccacgcc cctgggcggg caaccgtcca cgacacccac gatgccgcgg ccctgtgtgg
     2761 tttcggcaat cagcacttga gtcgggttgg cggtggcgca gaagacccgg cagacctcgg
     2821 gcaccatttt gacggcattg agcacgttga cgggataaaa cccctcgccg aggaacaaga
     2881 tgaagctgtg cccggcgccg atggccagtg cattctttct cgccaactcg atcatggccg
     2941 gatcggtgcc ggtccaacgc acgaggcatt tgccggacgc ctcgcagaag gccagtccga
     3001 acttgatgcc gggcaccgca ccgaccagag cttcgtggag gtcctccact gacttgatga
     3061 aatgagtctg gccgagaatg aagttcacgg cttcaggctt ctcgatctga atgaccttga
     3121 gtttggtttc catagcggtc agatggttgc gcgacgtcgg tgtccggtca agccgggaac
     3181 gatgctcttc aacgcgagtt tttaaacagg ctctcaggaa ttgctctgcc gggaagcttg
     3241 agttgctgct ccgctggatg tagataattg gatgtgaacg aatatatttt ggattcccgc
     3301 caggctgcct cggccaaaca gtttgagcgg cagagcgacc gttatgggaa gtcgcacatt
     3361 ctggcaaata ccgaggacgt ggaagcggca ttgaaaggca ttagcgtgcc ggtcggcggg
     3421 gcggccctgg acatcgccac tggcgggggg cacacggcgc tctggctcgc ccgtcatggc
     3481 tggaaggtca cggcgggtga tatcgcgccg cgcatgctga agaacgcggc ccagttgtgt
     3541 gccgaagccg ggctgagcat cgaaacgaag ctgtgcccgg cggagaagct gccgtttggc
     3601 gcgggtgcct ttgagctagt caccgcgcga gtggcgccgc atcacttcag ttcgcccgcg
     3661 cggttcgtag gcgaggtcgc gcgtgtgctc aagccgggtg gacgctttct attgattgac
     3721 gggagcgtgc ctgacgacga cccgccgaca gaggaatggc tgcatcacgt ggagaagttg
     3781 cgcgacccga gccatggccg ctatctctct cgcaagacct gggaagggct ggttcgagac
     3841 gcgggcttga ccgttgagcg ttccgaactg caaccataca aacagccgga cctgcaatgg
     3901 tatttcgaga ctgccgccac cccgcccggg aaccgcgagc aggtaatggc ggcagtgggc
     3961 ggcgcagcca agcaggtgcg agcgg
//
LOCUS       DATCGS010000379         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_181637, whole genome shotgun sequence.
ACCESSION   DATCGS010000379 DATCGS010000000
VERSION     DATCGS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181637"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2215
                     /locus_tag="VIL39_07805"
     CDS             <1..>2215
                     /locus_tag="VIL39_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEY5481368.1"
                     /translation="RDVKKLQPERSIRLIHRLHETRMKTITEAWKEYPGPFDFSYKYS
                     VAHMYSSPTPSFANPMLAELPAGMRTWMTVRNDDIYSFRWGDPEYARAYVKALPGPDK
                     LAGYYMGPDGYIWGREFISTEPDTPRQLVIKQQWYSFMLWGRLSYEPALPDALFQRAL
                     AARFPEAPGDKLFSASSSASKIIPQITRFFWGDIDLKWFPEGCLSRPSYRGWYTVKHF
                     MEGDTMPGCGIANIRTWRERVLAGKAVEGITPLQVADALETDARTTLQLLEAMPQTVT
                     NKELRLTIGDLKAMAYLGDYYAEKIRGAADLALFDKSAQTGQRDSAVRHLDAALEHWR
                     RYAAVATSQYRPQLLTRVGYLDLNALTENVAADIAMAKEWQPGTLEPGPASRQRGEAR
                     AQAGSGSRISRWQPHDFTFTNRAEPANPFRVPFSAEVTGPNNAKLTIPGFYDGNGTWK
                     IRVSPAAEGKWQLVTHSTVPAIDGQQVQFVCTPNGAQGHGGVRVDAQHPRHFVCEDGT
                     RFFPLGYECDWLWALDATNAELPTVNRFLDKLAANGFNYVLLNAYAHDTSWAKGKTSD
                     DDYGPPPLYAWAGTNEQPDHSRFNLAYWQHYDRVIAALNDRGMVAHVMIKVYNKMVNW
                     PAKGSAEDDLYFHWLIARYCAYPNVHWDFSKESNNEKDLDYKLGRIKFIRDNDPYRRP
                     ITTHTDLQTYDSGAYNGVLDYRSDQVHSKWHASLLKHREQHAWPVLNVEFGYEHGP"
BASE COUNT          455 a          685 c          681 g          394 t
ORIGIN      
        1 cgtgacgtga agaagctgca gcccgagcgg tccatccgtc tcattcatcg gctccacgag
       61 accaggatga aaaccatcac ggaggcgtgg aaggaatatc ccggcccctt cgacttcagc
      121 tacaagtact cagtggccca catgtattcc tcgcccacgc cgtcgttcgc caatccgatg
      181 ctcgccgagc tgccggcggg gatgcgcacc tggatgacgg ttcggaacga tgacatttac
      241 agcttccgct ggggcgaccc cgaatacgct cgcgcctacg tcaaggcgct cccgggtccc
      301 gacaagctgg ccggctacta catggggcct gacggctata tctggggccg ggagttcatc
      361 agcaccgagc cggacacgcc gcggcaattg gtgataaagc agcagtggta tagcttcatg
      421 ctgtgggggc ggctgagcta tgagccggcc ttgccggacg ctttgtttca gcgcgctttg
      481 gcggcccggt tcccggaagc gcccggagac aagctgttca gcgcctcctc atcggcgtcg
      541 aagatcatcc cacagattac tcgcttcttc tggggggaca ttgatctcaa gtggttcccg
      601 gagggctgcc tcagccgccc gagttacagg ggttggtaca ccgtgaagca cttcatggaa
      661 ggcgacacga tgccgggctg cggcatcgcc aacatccgca cctggcgcga gcgggtcctg
      721 gcgggaaaag cggtggaggg catcactccg ttgcaggtgg cggatgcgct ggaaaccgat
      781 gcgcgcacga cactccaact cctggaggca atgccgcaaa ctgtcactaa taaggaactc
      841 cgcctgacta tcggcgacct gaaggccatg gcttacctgg gagattatta cgctgagaag
      901 atccgtggcg ctgccgacct ggccctgttc gacaaatccg cccagaccgg gcaacgggac
      961 tcggcggtgc gacacttgga cgcggcgttg gaacactggc gccgatacgc tgccgtagcc
     1021 acgagccaat accgcccgca attgctgaca cgtgtcggct acctggacct aaatgccctg
     1081 acggaaaatg tagccgccga cattgcgatg gcgaaggagt ggcagccggg gacgcttgag
     1141 cccggcccag cttcgcgcca gcgaggcgag gcaagagccc aagccggctc gggaagtcgc
     1201 atctcgcggt ggcaaccgca cgatttcacc ttcaccaacc gggcagagcc ggccaatccg
     1261 ttccgggtgc ctttctcggc cgaggtcact ggcccgaaca acgccaagct gacgatcccg
     1321 ggtttctacg acggtaacgg gacctggaag atacgagtct cgcccgcggc ggaaggcaag
     1381 tggcagcttg tgacccattc gaccgtgccg gcgattgatg gccagcaagt gcagttcgtc
     1441 tgcacgccta acggggcgca ggggcatggc ggtgtgcgag tggatgcgca gcatccgcgc
     1501 catttcgtct gcgaggatgg cacgcgcttt ttcccactgg gctatgaatg tgactggctg
     1561 tgggcgctgg atgcgactaa cgccgagttg ccgaccgtga accggttcct ggacaagctt
     1621 gcggccaacg gcttcaacta cgtgctgctc aatgcctacg cccatgacac ctcttgggcc
     1681 aaaggcaaaa cgagcgacga cgattacggg ccgccaccgt tgtatgcgtg ggcagggacc
     1741 aacgagcagc ccgatcatag ccggttcaat ctggcttact ggcagcatta cgaccgggtc
     1801 atcgcggcgc tcaatgaccg tggcatggtc gcccacgtga tgatcaaggt gtataacaag
     1861 atggtgaact ggcccgccaa gggcagcgct gaggacgacc tgtatttcca ctggctgatc
     1921 gctcgttact gcgcctaccc gaatgtgcat tgggatttct cgaaggaatc gaacaacgag
     1981 aaagacctgg actacaagct cgggcggatc aagttcatcc gtgacaacga cccctaccgc
     2041 cggccgatca cgacccacac cgaccttcag acctacgaca gcggcgctta caacggagta
     2101 ttggactacc gcagcgacca ggtgcattcc aaatggcacg cctcgctcct caagcatcgc
     2161 gagcagcacg cctggccggt gctcaacgtg gaattcggct atgagcatgg cccgc
//
LOCUS       DATCGS010000380         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_30179, whole genome shotgun sequence.
ACCESSION   DATCGS010000380 DATCGS010000000
VERSION     DATCGS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30179"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..472
                     /locus_tag="VIL39_07810"
     CDS             197..472
                     /locus_tag="VIL39_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481369.1"
                     /translation="MKAVTINISLNPELAEFARSDSEAHAFDSMSEYMRDLIRNRRQE
                     QIKQDVALLEAAIAGAPLGDPSDEEMADIVKTQHRLRAERKRRARRS"
     gene            456..863
                     /locus_tag="VIL39_07815"
     CDS             456..863
                     /locus_tag="VIL39_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEY5481370.1"
                     /translation="MRVVLDANVVFAGAGWRGEAHNCLLAMAKRRITVFATEEVLGEL
                     RALVAERGSKFKHAPHNLLTWYYDRVKLVEPAPLGKQRSRDAKDDPYLACALAAGATI
                     IVSRDDDLLALEKPFGIQIVTPRQLLAKLTRPI"
     gene            complement(1048..>2834)
                     /locus_tag="VIL39_07820"
     CDS             complement(1048..>2834)
                     /locus_tag="VIL39_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5481371.1"
                     /translation="TTLTGYTLAEAVGKNLRDLVKSGQQERAVYEEMWATILAGQGWY
                     GQLVNRRKDGSLYPEEQTITPVRDANGEITHFIAIKQDLSERKQAEQAMRVSEERYRT
                     LAETAQDIIYVIDREFRMTYINEYGARHFGHSLPEMIGRHLTELFPPETTQWKLADVA
                     DVFRTGESLYQERQLSTGPRLMWLDSRLVPIKNATGEVESILGISRDITEHKQVEGQR
                     LRTQRLESIGTLAGGVAHDLNNALAPILLAAELLRLEFPEMAGTYLDLIQAGAQRGAA
                     MVKQLLTFAKGVEGERLLIQPKHLLKEMEKLIGSTFPKNIQLQTRYAKDLRTILGDVT
                     QLHQVLLNLCINARDAMPEGGTLTLEAENMELETVYADALMEAKPGHYVVWRVMDTGT
                     GIPPEILDRIFDPFFTTKDPGKGTGLGLSTLIGIVKSHGGFVRVYSVPGKGSTFAVYL
                     PAAASSADNTPLPTETDMPFCGHGETILVVDDEAAVRSVLRAILTKLNFKVLTASDGA
                     DALTQVTEQSAELRAVITDVHMPHMDGLSFVRVLKGRLPQVGIIVASGRLDEPDENEF
                     KKLGVSALLDKPFSQKELVEALKTVFQK"
BASE COUNT          540 a          810 c          884 g          600 t
ORIGIN      
        1 ataatagccc ttatggggag ttccccataa gggctattct cgaccatccc tccgggatga
       61 ggagggtcaa ttcctagcgg cattgggcct tccagttagc gcaaggcggg gcgaggattg
      121 gagagagaat tcctccaaac aagatttcgc gcttggcagg ctttgacatg ctttgtcaat
      181 gaggtatttt gctggcatga aagcagtgac cattaacatc tccttgaacc cggagctggc
      241 agagttcgct cggtcggatt cggaggcgca cgcatttgat tccatgagcg aatatatgcg
      301 cgacttgatt cgcaaccggc ggcaggagca aatcaagcag gacgtggcct tattggaagc
      361 ggctatcgcg ggcgcgccgc tgggtgatcc ctcggatgag gaaatggccg acatcgtgaa
      421 gacccagcat cgcctccggg cggagaggaa aaggcgtgcg cgtcgttctt gatgccaacg
      481 tcgtcttcgc tggtgcgggc tggcgcggtg aagctcacaa ttgcctgctg gcgatggcta
      541 agaggcgaat caccgttttt gcaacggagg aagttctggg agaactgcgg gcgctggtgg
      601 cggagcgggg aagcaaattc aaacatgcgc cgcataattt gctgacgtgg tattacgacc
      661 gggtgaaact ggttgagcct gcaccgttgg gcaagcaacg cagccgggat gcaaaagacg
      721 atccttattt ggcctgcgcc ttggctgctg gggcaacaat catagtttca cgcgatgacg
      781 atctgctggc tttggagaag ccttttggaa ttcagatcgt caccccgcgc caactgctgg
      841 caaaattgac gcggccgatt taggctttct tgccctcaat cggccagcga ggtgcgcttc
      901 ggcatagtgc atccaaaaat gagaacgttg cgcgtaggga tcggcgatgt gcgcgcggcc
      961 ccaccattgc acctttttca ggaatgcagg gcaggccagg accgccaggc gcaactcctc
     1021 ccagtccttg cgcttgcaaa tctcatttca tttctgaaaa actgtcttca acgcttccac
     1081 caattccttt tgactgaacg gcttatcgag cagcgcgctg actcccagct tcttgaactc
     1141 gttctcatcc ggttcgtcca gccgtccgct cgcgacgatg atgccaacct ggggcaaccg
     1201 gcctttgagc acccgcacaa aggagaggcc gtccatgtgg ggcatgtgca cgtcggtaat
     1261 caccgcgcgc agttccgccg attgctccgt cacctgagtc agcgcgtcgg cgccgtcgga
     1321 cgcagtgagc actttgaagt ttagcttcgt cagtatggcg cgcaggacgc tgcgcacggc
     1381 ggcctcgtca tccaccacga ggatggtctc gccgtggcca cagaagggca tatcggtttc
     1441 ggtcggcaag ggggtgttat cggcgctgga agcggcggcg ggcaggtaaa cggcgaaggt
     1501 ggaacctttt cccggcacgg agtaaacccg gacaaagccg ccatggctct tgacgatgcc
     1561 gataagggtg gaaagtccga ggcccgtgcc tttccccgga tccttggtgg tgaagaacgg
     1621 atcgaagatg cggtccagaa tctccggggg gatgccggtg ccggtgtcca tgacccgcca
     1681 cacgacataa tggccgggct tggcctccat gagggcgtcg gcatagacag tctcaagctc
     1741 catgttctcc gcttccaggg tcagcgtgcc gccctcgggc atggcatcgc gggcgttgat
     1801 gcacagattg agcaacacct ggtggagttg cgtgacatcg cccaggatgg tccggaggtc
     1861 cttcgcataa cgggtctgta gttggatgtt cttggggaag gtgctcccga ttagtttctc
     1921 catctccttg agcaggtgct tcggctggat gaggaggcgt tcgccctcga cgcctttggc
     1981 gaaggtgagg agttgtttca ccatggccgc cccgcgctgg gcgccggctt gaatgaggtc
     2041 gagataagtg cccgccatct ccgggaactc caggcgcaac aattcggcgg ccagcagaat
     2101 gggcgcgagc gcgttgttga ggtcatgcgc cacgccaccg gccagcgtgc cgatgctctc
     2161 cagccgctgc gtgcgcagcc tctgaccctc gacctgcttg tgctcggtaa tgtcgcggga
     2221 gattcccagg attgactcga cttcgccggt ggcgtttttg atgggcacca accggctgtc
     2281 cagccacatc agccgtgggc cggtagaaag ctggcgctcc tggtaaagcg actcgcccgt
     2341 gcggaagacg tctgccacgt cagccaattt ccactgcgtc gtttcgggcg gaaacaactc
     2401 cgtcagatgc cgcccgatca tctcaggcaa tgagtgtcca aaatgtctgg ccccatactc
     2461 attgatgtag gtcatgcgga actcacggtc aatgacgtaa ataatatctt gggccgtctc
     2521 cgccagggtg cggtagcgct cctcgctcac gcgcatcgcc tgctccgcct gcttgcgctc
     2581 ggaaagatcc tgcttgatcg cgatgaagtg ggtgatctcg ccgttggcat cgcgcaccgg
     2641 cgtgatggtt tgctcctcgg gatagaggct gccgtccttg cggcggttca cgagctgccc
     2701 gtaccagccc tggccggcga gaatcgtcgc ccacatctcc tcgtaaacgg cgcgctcctg
     2761 ctggccggac ttgaccaagt cgcgcaagtt tttgccgacg gcctccgcga gagtgtagcc
     2821 ggtcagggtg gtga
//
LOCUS       DATCGS010000381         9414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_478465, whole genome shotgun sequence.
ACCESSION   DATCGS010000381 DATCGS010000000
VERSION     DATCGS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478465"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VIL39_07825"
     CDS             <1..751
                     /locus_tag="VIL39_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481372.1"
                     /translation="GQWMKANGQAVYGTTASPFKRLPWGRCTKKLTPDGAILYLHVFN
                     WPSDGKLVVPGLKNVAQQAYMLTDPAKKTLAMQSSAQGLILSVPAAAPDPVSSTIVLK
                     VKGALDIEQPGLAQDYDGSVVLPASEARLHGDEIKYESGDQRNSLGFWTDPADWADWT
                     VEVTRPGRFDVTGEIASLEKASLEVSAGDSRSSGAVPVTGDYAKFKVAKLGVLEITAP
                     GKVTLAVRPVKDGWHPVNLRAIRLKPAAAAQ"
     gene            837..913
                     /locus_tag="VIL39_07830"
     tRNA            837..913
                     /locus_tag="VIL39_07830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:871..873,aa:Arg,seq:acg)
     gene            1129..1959
                     /locus_tag="VIL39_07835"
     CDS             1129..1959
                     /locus_tag="VIL39_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5481373.1"
                     /translation="MKFAVCNEMFEKNSIAETCAIASKLGYQGVEIAPYTLAGTADGV
                     TAAQRAEIRKAIQDNGLQTVGLHWLLAKTTGLHMTTPDDAVYHRTKDYFNVLIDLCHD
                     VGGKVMVIGSPKQRSLVDGQSYEGGWKRAVEMFRGACDKARDAGVTLCIEPLGKAETN
                     FINTVAEALKMMREINHPNFKVHLDVKAMAAEGKPSIPDIIRSVRAEDIGHFHVNDSN
                     LYGPGMGDIDYGPIAAAVREIGWNKWLSVEVFKYDPDPTTIARKSIECLKRYSWSRVA
                     "
     gene            complement(2004..4292)
                     /locus_tag="VIL39_07840"
     CDS             complement(2004..4292)
                     /locus_tag="VIL39_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY5481374.1"
                     /translation="MKTLSIVGAATALFITSLSLSCHADLPRPEHPRPDFQRVSWLNL
                     NGEWQFEVDSKAEGEQRGWISGRDLPQRIVVPFCPESKLSGLGLATNYLPHVWYRRQF
                     QVPAALRGQRLLLNFGAVDYTARVWLNGQFLGEHRGGYTPFAFEVTSGLRDGDNELVV
                     HVIDELRSGLQPGGKQSHGKSEGCVYTRTTGIWQTVWLEAVGETYLSEFALTPDLDGG
                     RIILQGSLNGPAKSVTLRVRAYADGHLVGEDTVPAAWRSTITVLKLKRVVPWQAGKPF
                     LYDLTIDTLRDGKVLDSVRSYCGLRKITIEGNRFLINDRPVFQRLILDQGFYPDGIYT
                     APSDEALRRDIELSMAAGFNGARLHQKVFEPRTLYWADKLGYLVWGEFPSWGVNIAHE
                     EAVRRFVDEWGEVVRRDRNHPALIGWCPLNETGTEEHVARLETLLATTRTMDSTRPFL
                     DSSGYTHFLPESDVFDAHDYEQSPEAFAARYAMFSATGCDPWHNHPADPRSRYRGQPF
                     FVSEYGGIHVKTARDTGAGWGYDGAGLSADDFLARYKGLTDVLLDNPNMFGFCYTQLT
                     DVEQEQNGIYFYDRAPKYDVQRLRAINQRPAAYETQPPRVRRVTWRTLVPTSQESAQI
                     WRYTTNAPSADWLKPGFDDSGWTQGKGGFGSPGTTGAVVGTEWRTSDIWIRRTFRADT
                     AKFALVALKLHHDEDTEIYLNGHRVAVFTGFVADYFTALADALSKAIVVGDNCLAIHC
                     HNTVGGQFIDAGIEVGTELKKP"
     gene            complement(4408..6684)
                     /locus_tag="VIL39_07845"
     CDS             complement(4408..6684)
                     /locus_tag="VIL39_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY5481375.1"
                     /translation="MRVDKAFRLSMGTLAFFAGLRLLGLESVRGADEPPSWQPAAAPL
                     MTRWAAQVSPTNALPEYPRPQLVRPDWLNLNGLWDYAITPSSMVKPPAFEGRILVPFP
                     VESALSGVMRRLDEKSTLWYHRRFAVPRAWSGQRVRLHFGAADWRAQVWVNGKGIGQH
                     RGGYDAFTFDITESLKRAGEEELLVAITDPTDGDQPRGKQSRKPEGIFYTPTSGLWQT
                     VWLEPVPGVCIDDLSLVPDRDASALQLRVGANSLADSVSVEAVALAAGREAGRVSGPA
                     NQELRLVIASPHLWSPDDPFLYDLRVTLKEGERVLDTVGSYFGLRQVALRKDAEGITR
                     LALNDQFLFQMGALDQGFWPDGLYTAPTDEALRYDIELLKQAGFNLARKHVKVEPDRW
                     YYWCDKLGLLVWQDMPSGQNTTPEGRIQFETELQRLVEGRRNHPSIILWVLFNEGWGQ
                     YDTERLTQWLKTLDPSRLVDDASGWTDKRVGDIIDAHSYPGPESPQPESERAAALGEF
                     GGLGLGVDGHTWSKRFWGYQAMPDQQSLTGRYASLLARVWTLHNHFGLSAAVYTQTTD
                     VETECNGLLTYDRAVCKLDLPQMRAANRGENQQPRFRVIASDALYGRPTWSYALERPA
                     TDWFKPGFNAAGWKEGIAGFGTQKTPGALVHTTWDTADIWLRREFKLDHEDLSNARFQ
                     IHHDEDAEIYLNGVLAASAAGFVAEYYELNISPEAAATLKPGANLLAVHCHQTEGGQY
                     IDVGIVAPQPAGAAALHK"
     gene            complement(6851..7483)
                     /locus_tag="VIL39_07850"
     CDS             complement(6851..7483)
                     /locus_tag="VIL39_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5481376.1"
                     /translation="MSTFIKVAIVDDEPDLRENIAGFVDAAKGIRCISAYPSGEEALA
                     HLPQDQPDVVLMDINLGGMSGIECVRQLKPRMPAVQIVMLTVFEDTEKIFSALAAGAS
                     GYLLKRLEPAKLLEAIREVHAGGSPMSAPIARKVVQSLQAAPARSNDNAGLSPREREV
                     LNGLAEGQAYKQIADRLGVSIHTVRNYIRRIYEKLHVQSRTEAVAKFLGR"
     gene            complement(7480..>9414)
                     /locus_tag="VIL39_07855"
     CDS             complement(7480..>9414)
                     /locus_tag="VIL39_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5481377.1"
                     /translation="CAGADSSLWVGTPGAGLYCVKGQRMLHYTTADGLSDNAVLALAS
                     GPDGTVWAGTMSGGLHRFAKGTLLSFGTGAGLSGQPVSALLPARSGGLWLGTGGGEVL
                     RGHGEQFRRLAGAGTLAGKAIRAMYEDNRGRLWLGTAGGRLACLSGRRCRSFDATPGL
                     QEDSVTGILGNQDGDVWFATSKGIYRVAGTDVTGWLSGQSPLRPQSIFETEAPAGATS
                     ACGWPRALSSPDGRLWFATMTGVVTFEPLGLEPGSAPPPVRLEAILVNGRPLVPTAGS
                     GTGTPDQSQPADRFTNNAAPLRLPSNLQSLDIQFTAFCFAAPEKVRFRHKLEGSDADW
                     VDGGVERWVHYGRLPSGSYDFRVTACNAAGVWNQTGAGFAFRIPPPAWRAAWALALYG
                     CAAIGLVAGTARLVSYRRLRGRLTRLAQEQAMERERMRIAKDMHDEIGSKLTRISYMS
                     ELAKGQLQGQEPVAVKLDAIAHTSRDLLQSLDEIVWVVNPRNDTLEHLAAYLGHYATE
                     YLQNTSVECELHIPRDLPHQPLSAETRHNLFLAFEEALNNALKHGRASQVRVAMAAGR
                     SRFEITIHDNGCGFDLANVSAAAASQGAARPPHGGNGLRNMRQRLVDVGGQCAICSEP
                     GQGSTVSLSVPLSGKNQGIL"
BASE COUNT         1826 a         2974 c         2866 g         1748 t
ORIGIN      
        1 cggccagtgg atgaaggcaa acgggcaggc cgtctatggc accaccgcca gtccgttcaa
       61 acgcctgcct tgggggcgtt gcacgaagaa gctcaccccc gacggggcga tcctttacct
      121 ccatgtcttc aactggccga gcgacggcaa gctcgtcgtg ccggggctta agaacgtcgc
      181 tcagcaggct tatatgctca ccgatccggc gaagaagacg ctcgccatgc aaagcagcgc
      241 gcaagggctg atcctgtccg tccccgcagc cgcgcctgac cccgtttcct ccaccattgt
      301 tctcaaagtc aaaggcgcac tcgacatcga acagcccggc ctggcacaag actacgacgg
      361 ctccgtcgtt ctgcccgcca gcgaagcccg tctccacggc gacgaaatca aatacgaaag
      421 cggtgatcag cgcaacagcc tcggcttctg gaccgacccc gccgattggg cggactggac
      481 cgtcgaggtc acccggccgg gcagattcga cgtaacgggc gaaatcgcct cgctcgaaaa
      541 agcctcgctc gaagtttccg ccggcgacag ccgcagcagc ggggccgtcc ccgtcaccgg
      601 cgactacgcc aagttcaagg tcgcgaagct cggcgtgctc gagatcaccg cgcctggcaa
      661 ggtgaccctc gccgtccggc cagtcaaaga cggctggcac ccggtgaatt tgagagcgat
      721 ccggctgaag cccgctgccg ccgctcagta aggcccaatg gccagaggcg caaatacggc
      781 gcaaatacct cttgcgcggg cgtggctcga cctgtagttt cctgtgccct ttgggcgcac
      841 ccatagctca attggataga gcgtctgact acggatcaga aggtttgtgg ttcaactcca
      901 catgggtgca ccaccttaga accagggtca cagcaacaca gaacaaccgc cagccagtct
      961 gagcggagaa tatgcatgtc atacagtaat atttccaaga cgaatcgccg gagcttcgtg
     1021 gtcgctgccg gcggtggtgc agtgggtctg gccctgtttc caggaccctg cttgtggtcc
     1081 gctgcgcagc ctggcgccgg ctccaccgga ccgagctctg tggaaaagat gaagtttgcg
     1141 gtctgcaacg agatgtttga aaagaactcc attgccgaga cttgcgcgat cgcttcgaag
     1201 ctaggttatc aaggcgtcga aatcgctccc tataccttgg ctgggacggc cgacggtgtg
     1261 actgcggcac aacgggcgga aattcgcaaa gccatccagg ataacggcct tcagaccgtc
     1321 ggcctgcatt ggcttctcgc gaaaactacc ggcctccaca tgacgacgcc ggacgacgcg
     1381 gtgtaccaca gaaccaaaga ctatttcaac gtcctgattg atctctgcca cgatgttggg
     1441 ggaaaagtga tggtgatcgg atctcccaag caacggagcc ttgtcgatgg gcagagctat
     1501 gagggagggt ggaaacgcgc agtcgagatg ttccggggag cctgtgacaa agccagggac
     1561 gccggcgtga cgctttgcat cgagccgctc gggaaagcgg agaccaactt catcaacacg
     1621 gtcgccgaag ctctaaagat gatgcgcgaa atcaatcacc ccaacttcaa ggtgcatctc
     1681 gacgtcaaag cgatggcggc ggaaggcaag ccttcgattc ctgacatcat tcgctccgtc
     1741 agagctgaag acattggtca tttccacgtc aatgattcca acctctatgg ccctggaatg
     1801 ggtgacattg attatggccc catcgcggcg gcggtcaggg agatcggttg gaacaagtgg
     1861 ctcagtgttg aggttttcaa atacgatcct gatccgacaa ctatcgcccg caagagcatc
     1921 gagtgtctga agcgctattc ctggtcgcga gtagcttagt agaatttcag cagcccgccg
     1981 agacgctcac cgcaccatcg tcgctacggt ttcttgagtt ccgttcccac ctcgatgcct
     2041 gcgtctatga actggccgcc gaccgtgttg tggcaatgga tggccaggca gttgtcgccc
     2101 acgacgatgg ccttggacag cgcgtcagcc agtgctgtga agtagtccgc aacgaaacca
     2161 gtaaacacag ccacccggtg gccgttaaga tagatctccg tgtcctcatc gtgatggagc
     2221 ttgagcgcaa ccaaggcgaa ctttgccgtg tcggcgcgga acgtccgtcg aatccagatg
     2281 tcgctcgtgc gccattcggt gccgacgact gcgccagtgg tgcccggcga accaaagccg
     2341 ccttttccct gtgtccagcc ggaatcatcg aagcccggct tcagccagtc ggcagacggt
     2401 gcgttcgtcg tgtagcgcca tatctgcgcc gactcctgcg aggtcggcac cagcgttcgc
     2461 cacgtcacgc ggcggactcg cggcggctgc gtttcgtagg cggcaggccg ctggttgatg
     2521 gcccgcagcc gctgcacatc atatttgggg gcgcggtcgt agaaatagat gccgttttgc
     2581 tcctgctcca cgtccgtcaa ctgggtgtag cagaagccga acatgttcgg gttgtcgagc
     2641 aacacgtccg tcaacccctt gtaacgcgcc aggaagtcat ccgcactcaa acccgccccg
     2701 tcatagcccc agccggcgcc cgtgtcgcgg gcggtcttga cgtggatgcc cccgtattcg
     2761 ctgacgaaga acggctggcc acgatagcgg ctgcgcgggt cggcaggatg attgtgccac
     2821 ggatcgcagc cggtcgcgct gaacatggcg tagcgggcgg cgaaggcttc tggactctgt
     2881 tcgtagtcgt gggcatcaaa gacatccgat tccggcagga aatgcgtgta gccgctcgaa
     2941 tcgagaaacg ggcgcgtcga gtccattgtg cgcgtggttg ccagcaaggt ttcgaggcgg
     3001 gcaacgtgtt cctcggtgcc ggtttcgttg agcgggcacc aaccaatgag ggccggatga
     3061 ttgcggtcgc ggcgcacgac ctcgccccac tcgtccacaa aacggcgcac ggcttcttcg
     3121 tgcgcgatat tcactcccca actgggaaac tcgccccaca ccagatagcc cagcttgtcc
     3181 gcccagtaga gggtgcgcgg ctcgaagacc ttctggtgga gccgcgcccc gttgaacccg
     3241 gctgccattg aaagctcgat gtcacggcgg agcgcctcgt cactgggtgc ggtgtaaatg
     3301 ccatcggggt agaagccttg gtcaaggatg agccgctgga agacaggccg gtcgttgatg
     3361 agaaagcgat tcccctcgat ggtgattttg cggaggccac agtagctgcg cacgctgtcc
     3421 agcaccttgc cgtcgcgcag ggtgtctata gtcaggtcgt agaggaatgg cttgcccgcc
     3481 tgccacggca cgacccgttt gagcttcagg acggtgatgg tcgagcgcca cgcggcgggc
     3541 acggtgtctt caccaaccag atggccgtcc gcgtaggccc gaacgcgcag cgttacactc
     3601 ttggccgggc cgttcaggga gccttgcaga atgatgcgcc cgccatcgag gtcaggcgtg
     3661 agggcaaact ccgacagata cgtctcaccc acggcttcca gccacacggt ctgccagatg
     3721 cccgtggtgc gggtatagac gcagccctcg ctcttgccgt ggctttgttt gccgcccggt
     3781 tgcaggccgg aacgaagctc gtcaatgaca tggaccacca gttcgttgtc gccgtcgcgc
     3841 aggccgctgg tgacttcaaa ggcaaaaggg gtgtagccgc cgcgatgctc accgaggaac
     3901 tggccattca accagacgcg ggcggtgtaa tccaccgcgc cgaagttcaa caaaagccgc
     3961 tgaccacgca atgccgcagg aacttggaac tgccgccgat accagacatg cggcaggtag
     4021 ttcgtcgcca gaccaaggcc cgatagtttg ctctccgggc agaagggcac cacgattctt
     4081 tgcgggaggt cacggcccga aatccagccg cgttgctccc cttcagcctt ggaatcaacc
     4141 tcgaactgcc actcgccgtt caggttcaac cacgatacgc gctggaaatc cgggcgcgga
     4201 tgttcgggcc ggggaagatc ggcgtggcaa gacaagctga gtgatgtgat gaaaagggcc
     4261 gtggccgcac cgactatgct aagggttttc atgactttgt ttgcgtcttc aataccgata
     4321 ttcagaacaa accgtattgc tgcgaagcag gggcaagcat cttcacttgc tcatctattc
     4381 cctgctcatc aattgccaac agcagaatca tttatgcaac gccgccgcgc cggcgggctg
     4441 gggcgccaca atgccgacat caatatattg gccgccctcg gtttgatgac aatggacggc
     4501 caagaggttg gcccccggct tgagcgtggc ggctgcttca gggctgatgt tcaactcgta
     4561 gtactcggcg acaaagcccg ccgcagaggc ggccaggaca ccattgaggt aaatctccgc
     4621 atcttcgtcg tgatgtattt ggaacctggc gttgctcagg tcctcatgat caagcttgaa
     4681 ttcccggcgc agccagatgt cggcggtgtc ccacgtggta tgcacgaggg cgcccggggt
     4741 cttctgcgtg ccgaaaccgg cgatcccttc tttccagcca gcagcattga atccaggctt
     4801 aaaccagtca gtcgcgggtc gttccagcgc gtaactccaa gtcggccggc catagagggc
     4861 atcggacgca atcacgcgga atcggggttg ctggttctcg ccgcgattgg cggccctcat
     4921 ttgcggcagg tcgagtttgc acaccgcccg gtcgtaggtc agcagtccgt tgcactccgt
     4981 ctcgacgtcg gtggtctggg tatatactgc ggcgctcaga ccaaaatgat tgtgcaaggt
     5041 ccagacgcga gccagaaggg aagcgtagcg accggtcagg ctctgctggt cgggcatggc
     5101 ctgatacccc caaaaacgct ttgaccaggt atggccgtcc acccccaaac ccagtccgcc
     5161 aaattccccg agcgccgccg cccgctcgct ctccggctgg ggggattccg gcccggggta
     5221 gctatgggcg tcgatgatgt cgcccacccg tttgtccgtc caaccgctgg cgtcatccac
     5281 cagccgggag gggtccagtg tcttgagcca ctgcgtcagc cgctccgtat catactggcc
     5341 ccagccttca ttgaagagca cccacaggat aatcgaggga tggttgcgcc gtccttcaac
     5401 caggcgctgc agttccgtct cgaattggat gcggccctcc ggggtagtgt tctggccact
     5461 cggcatgtcc tgccagacca gcaaacccag cttgtcgcac cagtaatacc accggtccgg
     5521 ctccaccttg acgtgtttgc gcgcgagatt gaagccggcc tgcttgagta actcgatgtc
     5581 gtaacgcagc gcctcatccg taggcgccgt gtataggccg tccggccaga atccctggtc
     5641 caacgcgccc atctgaaaga ggaactggtc attgagcgca aggcgcgtga tgccctccgc
     5701 gtctttgcgc aaggcgacct ggcgcaggcc gaaatagctg ccgacggtgt ccaggacgcg
     5761 ctcgccctcc ttgagcgtca cccgcaggtc gtagagaaac ggatcatcgg gcgaccatag
     5821 atgaggcgag gcgatgacca gccgcagttc ctggttggcc ggcccgctca cccggcccgc
     5881 ttcccggccc gcagccaacg ccacggcttc gacgctaacg gaatcagcca ggctattggc
     5941 accgactcta agctgcagcg cgctcgcgtc gcggtccgga accaggctca agtcatcaat
     6001 gcaaacacct ggcaccggtt ccagccaaac ggtctgccag aggccggaag tgggcgtgta
     6061 aaagatgccc tcgggtttac gcgactgttt gcctcgcggt tggtccccat ccgtcgggtc
     6121 tgttatcgcc accaggagtt cctcctcgcc cgcacgtttg aggctctcgg tgatgtcgaa
     6181 agtgaaggcg tcatagccac cccgatgctg accaattccc ttgccattga cccagacctg
     6241 cgcccgccag tctgccgccc caaagtgcag ccgaactcgc tgtcctgacc aagcccgcgg
     6301 cacggcgaac cggcgatgat accaaagcgt gctcttttca tcgagccgcc gcatcactcc
     6361 ggaaagggcc gactcgacag gaaagggaac cagaatccgg ccttcaaatg ccgggggttt
     6421 caccatcgag gacggcgtga tggcgtagtc ccacaagccg ttgagattca accagtccgg
     6481 ccgaaccagt tgcgggcggg ggtattcggg aagcgcgtta gtcggggaga cttgggcggc
     6541 ccagcgggtc atcaacggcg cggcggcagg ctgccaggaa ggcggctcat cggcgccgcg
     6601 aacgctctca agaccgagca atcgcagccc tgcaaagaag gcaagcgttc ccatgctcag
     6661 gcgaaacgcc ttatcaaccc tcattgtttg ccgcgtaatc ttcatcgaga tgattttgcc
     6721 ggaagggcag ttcttgtcac gtggcatgaa gtggccacag gacagccggg gtgttggagt
     6781 attggagcat tggagtgctg ataacccatc actccaatac tcctccactc cattcttcgc
     6841 cgccgcttct tcaccgcccc aggaatttgg ccaccgcctc ggtgcgcgat tgcacatgga
     6901 gtttctcgta gatgcggcgg atgtagttgc ggacggtgtg aatgcttacg ccaagacggt
     6961 cggcaatttg cttgtaggcc tggccctccg ccaagccatt gagcacctcg cgttcacggg
     7021 gggatagtcc ggcgttgtca ttgctcctcg cgggtgccgc ttgcagcgac tgcactacct
     7081 tgcgggcgat gggggcggac atgggagagc ctcctgcgtg gacttcgcgg atggcctcca
     7141 agagcttggc gggctccaat cgcttcaaca gatagccgct cgccccggcc gccagcgcgc
     7201 tgaagatctt ctcggtgtcc tcgaaaacag tcagcatgac aatctgcacg gccggcatcc
     7261 gcggcttaag ctgccgcaca cactcaatgc cgctcatgcc acccaggttg atgtccatca
     7321 gcaccacatc cggctggtcc tgcggcaagt gcgcgagggc ctcttcgccg ctcgggtagg
     7381 cgctgatgca acggatgcct ttggctgcat ccacaaaccc ggcgatgttc tcgcgcaggt
     7441 cgggttcgtc atccacaatc gcaaccttga tgaatgtgct cataaaatcc cttggttctt
     7501 gccacttagg ggcacgctca gactcacagt gctgccttgg cctggctcgc tgcagatcgc
     7561 gcattggccg cccacgtcaa ccaaccgctg tcgcatgttc cgaagcccgt ttccgccgtg
     7621 cggcggacgc gctgcgcctt gggaggcagc ggcagcactc acgttggcca aatcgaaacc
     7681 gcagccgttg tcatggatgg taatctcgaa gcgggaccga ccggcggcca tagcgacgcg
     7741 cacctgcgag gcgcggccat gtttgagcgc gttgttcaag gcctcctcga aggccaggaa
     7801 caggttgtgc cgggtttccg ccgagagcgg ctggtgcggc aggtcacgcg ggatgtgtaa
     7861 ttcgcactcg acggaggtgt tttgcagata ctcggtcgcg taatgcccca agtaggcggc
     7921 caagtgctcg agggtgtcgt tgcgcgggtt gaccacccag acaatttcgt ccaacgattg
     7981 gagcaagtcg cgcgaggtat gggcgatggc atcaagcttg accgccaccg gctcctggcc
     8041 ctgcaactga cctttggcca gttcgctcat gtatgaaatc ctggtcagct tcgagccaat
     8101 ctcatcgtgc atatccttgg cgatgcgcat gcgctcccgc tccatcgcct gctcctgggc
     8161 caggcgcgtc aggcgcccgc gcaagcggcg ataggagacc agccgcgccg tgccagcgac
     8221 caggccgatg gcggcacagc catacagcgc caaggcccag gccgcgcgcc aggccggcgg
     8281 cgggatccgg aaggcaaagc cggcgcccgt ttggttccat accccggcgg cgttgcaggc
     8341 ggtgactcgg aaatcgtaac tcccggacgg gagccggcca tagtgcaccc agcgttcgac
     8401 accgccatcc acccagtccg cgtcggagcc ctccaacttg tgccggaagc gcactttttc
     8461 cggcgccgcg aagcagaagg cggtgaattg gatgtccaag gactgcaggt tcgacggcaa
     8521 ccgcaggggg gcagcattgt ttgtaaaccg gtccgccggc tgcgactggt ccggagtgcc
     8581 tgtcccgctc ccggcggtgg ggacgagcgg tcggccgtta accagaatag cttcgaggcg
     8641 gaccggtggc ggcgctgaac cgggctccaa accgagcggt tcaaaagtca cgaccccggt
     8701 catcgtcgca aaccaaagcc ggccatcggg tgaactcagt gcgcgcggcc aaccgcacgc
     8761 ggaggtggcg ccagccgggg cctcggtctc gaaaatggac tggggccgca gtggagactg
     8821 gcccgatagc cacccggtta catccgtgcc ggcgacgcga tagattcctt tgcttgtggc
     8881 gaaccatacg tctccatcct ggtttcccag gatccccgtg actgagtcct cctgcaatcc
     8941 aggtgtcgca tcgaagcttc gacatcgccg acctgacagg caggccagtc tgccgcccgc
     9001 agtgcccagc cagagccggc cgcggttatc ctcgtacatc gcccgaatag ctttgcccgc
     9061 cagggtgccc gccccggcca gccgccggaa ttgttcgccg tggccgcgca ggacctcgcc
     9121 gccaccggta ccgagccaga ggccgccact gcgcgccggc aggagcgcgc tgaccggttg
     9181 gccggatagt cccgcaccgg tgccgaagct cagcagcgta cccttggcaa agcggtgcag
     9241 cccgccagac atcgtcccgg cccaaaccgt gccatcaggt ccagatgcca aggcgagaac
     9301 cgcgttgtcc gacaaaccgt cggccgtggt gtagtggagc atgcgttggc ccttgacgca
     9361 ataaagccct gcccccggag tgcccaccca gagactggaa tccgccccgg cgca
//
LOCUS       DATCGS010000382         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_527996, whole genome shotgun sequence.
ACCESSION   DATCGS010000382 DATCGS010000000
VERSION     DATCGS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527996"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIL39_07860"
     CDS             <1..261
                     /locus_tag="VIL39_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HEY5481378.1"
                     /translation="TVNEYVAADGKQAVVFALRHSQQYNTAAPTIRLRGLDERALYKV
                     ESVNHKLVDREAQFSGAYLMRAGLNVDLGGDFDGTAIILERQ"
     gene            643..1995
                     /locus_tag="VIL39_07865"
     CDS             643..1995
                     /locus_tag="VIL39_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5481379.1"
                     /translation="MSTLNSAEEKKPSYAAHIGLDWADQKHFWTMLTADGKCTRGDLK
                     QTPEAIEIWAAELAQRFGGRPIALALEQVRGALVAILSKYAHVHLFPVHPTKLAHYRL
                     SVCPSGAKGDPSDADLILDYLVKHPERLCCLEPDTMETRSLQFLVEERRKLVNQHTCQ
                     VQILIHWLKQIFPQMLEWFDNMSTPLVGDLLQRWPQLPNLQKASAKTLRRFFDQHNCR
                     SEERIQQRLEEIDQAVPATNDAALLRTGNLCIRISVQLLASLRAGIAEFDSQIEAIYL
                     IHPDRFITASLPGAGPALEPRLLAALGTNRERFESASNMACCFGIAPVTESSGKSRWV
                     HWRWACSKFVRQTLHEWANCSIRTCGWAREHYDKQRLKGKGHHASVRSVAFKWIRILF
                     RCWRDRLPYSEQRYLEALRTNGTAPSTPAPIVGESTSALPGTRWEGCGDFAKLAKVSS
                     "
BASE COUNT          445 a          655 c          617 g          418 t
ORIGIN      
        1 acggtgaatg agtacgtggc cgccgacggc aagcaggccg tggtattcgc cctgcggcac
       61 tcgcagcagt acaataccgc cgcgcccacc atacgcttgc gcgggctgga cgaacgcgcg
      121 ctgtacaaag tggaatcggt caaccataaa ctggtggacc gcgaggccca gttcagcggc
      181 gcctacctga tgcgcgccgg cctcaacgtg gacctcggcg gcgatttcga cggcaccgcg
      241 attattctgg agcgccagta acggtggcat cagttggccg gcaaccgctc ccttatagtt
      301 actccgtgag gaggacttta tataagggtt atccacagcc gtcgaaaccg gcggcctgtg
      361 gataaggcgt tcgctcaatt gccaatcggc gacggctggt ggcaaggtag gagggcgagc
      421 aggcaccccg cggatacgaa taccgctccc tgacaccgcg acgctattca cgtagcgtcg
      481 agaggtctac cgtgagagct tcgaccgcgg gatgcgcttt ggttccaaaa cgaatcctca
      541 gatgtcgtct ggtcgccaga ctctactata aggccttcgc ggaaacaaag tcctgccggc
      601 acgcacgacg acgacgcacc accgaaaagg aagctaagaa aagtgtccac actcaactcc
      661 gcagaagaga aaaagccatc ctacgcagcg cacatcggtc tggactgggc cgatcaaaaa
      721 cacttctgga ccatgctcac cgccgacggc aagtgtaccc gcggtgacct gaagcagaca
      781 ccggaagcca tagaaatctg ggccgccgag ctggcccaga ggttcggtgg ccggccgatc
      841 gccctggcgc tggagcaggt gcgcggtgcg ttggttgcca tattgtccaa atatgcccat
      901 gtgcacctgt tccccgtgca ccccaccaag ttagcccact accgtttgag cgtctgtcct
      961 tccggggcca agggcgatcc cagcgatgcc gatctcatcc tggactacct ggtcaagcat
     1021 ccagagcggc tctgctgtct ggagccggat acgatggaga cgcgcagcct gcagttcctc
     1081 gtcgaggagc gtcggaaact ggtcaatcag catacctgcc aggtacagat cctcatccat
     1141 tggctcaaac aaatcttccc gcagatgctg gagtggttcg acaatatgtc cacacctctg
     1201 gtaggagact tgctccagcg ctggccccaa ttgccgaact tgcagaaggc ctcggcgaaa
     1261 accctgcgca gattctttga ccagcacaac tgccgcagtg aggagcggat tcaacagcgg
     1321 ctggaggaga ttgaccaggc cgtgcccgcg acgaacgatg cagccctgct gaggaccggc
     1381 aatctatgca ttcggatctc ggttcaattg ctggcgagtc tgcgcgctgg tatcgccgag
     1441 tttgacagcc agatcgaggc catctatctg atccatcccg accgctttat taccgcatcg
     1501 ttaccaggag ccgggccggc gctggagccg cggctgctgg ccgccttggg aaccaaccgc
     1561 gagcgtttcg aatccgcctc caatatggcg tgttgtttcg gcatcgcccc ggtcacggaa
     1621 agcagtggca aatcccgctg ggttcactgg cgttgggcct gttccaaatt cgtgcggcag
     1681 acccttcatg aatgggcgaa ctgctcgatt cggacatgcg gctgggcccg ggaacactat
     1741 gacaagcagc gcctcaaagg caaaggtcat catgccagcg ttcgctccgt tgccttcaaa
     1801 tggatccgca tcctcttccg ttgttggcga gaccgtctgc cctattccga acaacgttat
     1861 ctggaagcac ttcgcactaa cggcactgcc ccatccacac ctgcgccgat cgtcggcgag
     1921 tccacatcag cccttcccgg cacccggtgg gaaggctgtg gagattttgc gaaactcgct
     1981 aaagtttctt cttgacggat taactcagat gtctcacggt cgcggctccg attggagttt
     2041 cggttcagtc aatcggagcc ccggccgtga gggcggggtt tcctgaatgg ccccggcatt
     2101 ttcatcgttc gcttgagacg ccgctggcgt catga
//
LOCUS       DATCGS010000383         6524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_30242, whole genome shotgun sequence.
ACCESSION   DATCGS010000383 DATCGS010000000
VERSION     DATCGS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30242"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VIL39_07870"
     CDS             complement(<1..796)
                     /locus_tag="VIL39_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5481380.1"
                     /translation="MKRHLLVAAAVGLIGIAAPAAQISGDRPNILWLVSEDNDTFLGC
                     YGDPLAHTPTLDRLASQGVLFERCFAQPVCAPSRFTLITGMYSVTCGPAEHMRARGKI
                     PGWVKGFPAYLRKAGYYTSNNAKTDYNSPINMKEAWDASNKNAHWRKRPDAKQPFFSV
                     FNHEVTHESCLFPVAERKLDFPPMDPAKVRIPPYQPDTPEIRADWARYYNHMTLMDEQ
                     IAAKLKELEKDGLADNTIVFYYSDNGGVLPRSKRFLQRSGTHVPLIV"
     gene            complement(946..1950)
                     /locus_tag="VIL39_07875"
     CDS             complement(946..1950)
                     /locus_tag="VIL39_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEY5481381.1"
                     /translation="MDLKTTYLGLKLRTPLVPAASPLSEEIDGIKQMEDAGASAVVLY
                     SLFEEQLRQDRAELDHHMEHGTESFAEALTYFPEPKDFRLGPEEYLKHIAQAKQAVRI
                     PVIASLNGSSAGGWTEYAKGIQQAGADALELNIYYIPTDMDLTSAQIEQTYLDILKAV
                     KSVVTIPVAVKLSPFFTNFANMAKRLDQAGANGLVLFNRFYQPDIDLEALEVKPNILL
                     STPMAMRVPLRWIALLYGKVRPSLAATSGVHRATDVLKMLMAGADITMLCSTLIRHGV
                     RQIGVIERDLIAWMEEHEYESVTQLKGSLSQKNCPDPSAFERAQYMRAISQYAAANSK
                     "
     gene            complement(2018..5635)
                     /gene="nifJ"
                     /locus_tag="VIL39_07880"
     CDS             complement(2018..5635)
                     /gene="nifJ"
                     /locus_tag="VIL39_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEY5481382.1"
                     /translation="MNKTYTTIDGNEAVAYVAYRCNEVMGIYPITPSSNMGEWCDQWA
                     SEGIKNLWGTIPSVVEMQSEGGAAGAVHGALQTGALTCTFTASQGLLLKVPNMLKIAG
                     ELTPTVFHITARTLATHALSIFGDHSDVMLARTTGFAMLSSASVQEAMDFALIAQAAT
                     LESRIPFLHFFDGFRTSHEVSKIELVTEDIIRKMINEEFVIAHRQRALNPDRPVLRGT
                     AQNPDVFFQAREACNPFYAKCADITVKAMDQFAALTGRQYKPFQYVGDPNAERVVILM
                     GSGCETADETADYLNERGGKVGVLKVRLYRPFCVKRFMEALPASVKALAVLDRTKESD
                     ALGEPLYLDIMAALNEGLAAGWGKLKSMPKVLGGRYGLASKDFTPAMVKAVFDNLQTA
                     HPKNHFTVGIDDDVTHTSLACDAEFSTESDKVVRALFYGLGSDGTVGANKNSIKIIGE
                     NTDNYAQGYFVYDSKKAGAVTISHLRFGPKPIRSIYLVSKANFIACHQPIFLDRYDML
                     KDLVPGGTFLLNSHYGQNEIWDTLPRPVQETLIKKKLKFYVIDALTVAKATGMGSRIN
                     TIMQTCFFALSGVLPRAEAIEQIKYTIKKTYGRKGEEVVKKNIAAVDQTLTNLFEVKV
                     PAKVTSTKELPAPFAADAPKEIADTLGLIYGNRGEELPVSVFNADGTFPTGTSRYEKR
                     NLALEIPVWDEKTCIQCGKCVMICPHACIRMKVYDATCLTGAPATFKSTDARAPEWKG
                     MKWTLQVAAEDCTGCCLCIEVCPAKNKTEAKLKAINMRPQPPIRFSERENFNFFLALP
                     EFDRRKVKLTQLRQMQVMQPLFEFSGACAGCGETPYLKLLTQLFGDRLVAANATGCSS
                     IYGGNLPTTPYAKNKEGRGPAWNNSLFEDNAEFGLGYRASIDKQKEFACELLQKLAPK
                     LGDDLVTGLITATQTDEAGIHDQRERLVALKQKLAGDNSSEAKMLLAIADNLARRSVW
                     IIGGDGWAYDIGYGGLDHVLASGRDVNVLVLDTEVYSNTGGQCSKSTPRGAVAKFAAG
                     GKSLRKKDLGLIAMSYGSVYVASVAMGAKDEHTLKAFLEAESYRGPSLIIAYSHCIAH
                     GIDLDRGAGVRQQRMAVESGQWLLYRYDPRRTEKGENPLQVDSQAAKTKLQDYLLSEN
                     RFKMLTKSKPEDAKKFFAQAQTDADKRYAYYQYLAGRDLKSGNDAVPQAKPTTSVDQQ
                     P"
     gene            complement(6015..>6524)
                     /locus_tag="VIL39_07885"
     CDS             complement(6015..>6524)
                     /locus_tag="VIL39_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481383.1"
                     /translation="RLQFSRDWFAVGRVLGNSLDMRQEIHYALTLTHWLALEGGRHDS
                     VEELWSDLVFAAQRFGYCIVRLALADGQRVWDQTNACGSAHSVVQVLQAGRLGTLELK
                     APACEFGALPHQRTQPCERSFCPRVSDERVFEIVSELLAEGWVKGVSKSSSAVAGPLR
                     FDTKLVRQQ"
BASE COUNT         1161 a         1949 c         2045 g         1369 t
ORIGIN      
        1 agacgatgag cggaacatgc gtgccgctgc gctgaagaaa ccgcttgcta cgggggagca
       61 cgccgccatt gtccgagtag tagaacacga tggtgttgtc agccaggccg tctttctcca
      121 actccttgag cttggcggcg atctgctcat ccatcagcgt catgtgattg taataccggg
      181 cccagtccgc gcgtatctcg ggcgtgtccg gctgataggg cgggatgcgc accttggcgg
      241 ggtccatagg tgggaagtcc agtttgcgct ccgcgacggg gaacaggcag ctctcatgtg
      301 taacttcgtg gttgaacacg ctgaagaacg gctgcttcgc atcgggccgc ttgcgccagt
      361 gggcattctt gttcgaggcg tcccaggcct ccttcatgtt gatgggcgag ttgtagtccg
      421 tcttggcgtt gttcgatgtg taatagccgg ccttgcgcaa ataggcggga aagcccttta
      481 cccaccccgg aattttgcct cgggcgcgca tgtgctcagc cgggccgcag gtgacggaat
      541 acatgccggt gatgagcgtg aaccgcgagg gtgcgcaaac cggttgggcg aagcagcgct
      601 cgaacagaac gccctggctg gccaggcgat cgagcgtcgg ggtatgcgcc agcggatcgc
      661 cgtagcaacc gaggaacgtg tcgttgtcct cgctcaccaa ccaaaggatg tttggccggt
      721 caccggagat ctgcgctgcc ggagcggcaa ttccaattaa tccaaccgct gctgcgacca
      781 ggagatgccg tttcattgcg ttcacaagtt ggagcttcac atgccgccgc gtcaccatcc
      841 aagcaaaaag ccgcccggct ctgtctctga gccgggcggc cttcttagtt tgttcttcgc
      901 ttccgctgca gtcccacacg ccgcgaccac agctcgcgcc gcgcactatt tggaattggc
      961 ggccgcgtac tgcgagatgg cccgcatgta ttgcgcgcgc tcgaaggcgc tcgggtcggg
     1021 gcagttcttc tggctcaggc tgcccttgag ctgagtgacg gattcgtatt cgtgctcttc
     1081 catccaggcg atgaggtcgc gctcgatgac gccgatctgg cggacgccgt gccggatgag
     1141 cgtggagcag agcatggtga tgtcggctcc ggccatgagc atcttaagga cgtccgtggc
     1201 gcggtggacg ccgctggtgg cggcgaggct gggccgcact ttgccataga gcaacgcgat
     1261 ccagcgcaac gggacgcgca tggccattgg ggtgctcagc aggatgttgg gcttcacttc
     1321 gagcgcctca aggtcgatgt ccggctggta gaaccggttg aacaggacga gcccgttcgc
     1381 gccggcctgg tccaggcgtt tggccatgtt ggcgaagttg gtgaagaacg gactgagctt
     1441 taccgcgacg gggatggtga ccacagactt gaccgcctta agaatatcga gataggtctg
     1501 ctcgatctgg gcggaggtga ggtccatgtc cgtcgggatg taatagatgt tcagctccag
     1561 ggcatcggcc ccggcctgct gaatgccctt cgcatactca gtccagccgc cggcagagga
     1621 cccattgagg ctggcgatga ccgggatgcg caccgcttgc ttggcctggg cgatgtgctt
     1681 gagatactcc tccggcccga ggcgaaagtc cttcggctcc gggaagtagg tgagtgcctc
     1741 cgcaaagctt tcggtgccat gctccatatg atggtccagc tcggcgcggt cctggcgcag
     1801 ttgctcctcg aacagggagt agagcaccac ggccgacgcc ccagcgtctt ccatctgctt
     1861 aatgccgtcg atttcttccg acagcggaga agccgcggga acgagtgggg tgcggagttt
     1921 cagaccgagg taagtagttt tgagatccat agtgttttac gtggttacaa cgttaaggcg
     1981 tcattcttga tacgtaatgc gtgatgcgtg atgcgtgtta gggctgctgg tccacagatg
     2041 tggttggttt tgcctggggg acagcgtcgt tgccggactt gaggtcgcgt cccgccaggt
     2101 attggtagta ggcgtaacgc ttgtcggcgt cggtctgcgc ctgggcaaag aacttcttgg
     2161 catcctccgg cttgctcttg gtgagcatct tgaaccggtt ctccgagagc aggtagtcct
     2221 gcagtttggt cttcgcggcc tgggaatcca cttgcagcgg gttctcgccc ttctccgtgc
     2281 gacgcggatc gtagcggtag agcagccatt gaccggactc aacggccatg cgttgctgcc
     2341 ggacgcctgc cccgcggtcc aggtcaatgc cgtgggcgat gcaatggctg taggcgatga
     2401 tgagtgacgg gccccgatac gattcggctt cgaggaacgc cttgagcgtg tgctcatcct
     2461 tggcgcccat ggcgacgctc gccacgtaca ccgagccgta gctcatggca atcaggccca
     2521 ggtctttctt gcgcaacgac ttgccgcctg cggcaaactt cgccaccgcg ccgcgcggcg
     2581 tcgatttcga gcactggccg cccgtgttgg aatacacttc ggtgtccagg acaagcacgt
     2641 tgacgtcgcg gccgctggcc aggacgtggt caagcccgcc gtaaccgatg tcataggccc
     2701 agccgtcacc gccgatgatc cagacgctgc gccgggccag gttgtccgcg atcgccagga
     2761 gcatcttcgc ttcactgctg ttgtcgccgg cgagcttctg tttgagggcg actaagcgtt
     2821 cacgctggtc gtggatgccg gcttcgtccg tctgggtggc tgtgatcagg ccggtgacca
     2881 ggtcgtcccc gagcttcggg gcgagcttct ggagcagctc acaagcgaac tctttctgct
     2941 tgtcaatgga agcgcgatag ccaaggccga actcggcatt gtcctcaaag agggagttgt
     3001 tccacgcggg gccgcggcct tccttgttct tcgcatacgg ggtggtgggc aggttgccgc
     3061 catagatgga ggagcagccg gttgcgttgg ccgcgacgag gcggtcgccg aaaagctggg
     3121 tgagcagctt caagtaaggg gtctcgccgc aaccggcgca cgccccgctg aattcgaaca
     3181 gcggctgcat cacctgcatc tggcggagct gggtcagctt aaccttccgg cggtcaaact
     3241 ccggcagcgc caggaagaag ttgaagttct cgcgctcgct gaagcggatt ggcggctgcg
     3301 ggcgcatgtt gatcgccttg agcttggcct cggtcttgtt cttggccggg cagacttcga
     3361 tgcacaggca gcagcccgtg caatcttccg cggcgacctg cagcgtccac ttcatcccct
     3421 tccactcggg cgcgcgcgcg tccgtgctct tgaaggtggc gggcgcgcct gtcaggcatg
     3481 tggcgtcata gactttcatg cggatgcagg cgtgcgggca gatcatcacg cacttgccgc
     3541 actggatgca ggtcttctcg tcccacaccg ggatttccag cgccaggttc cgcttctcat
     3601 agcgggacgt gccggtcggg aacgtgccgt cggcgttgaa cacgctgact ggcaattcct
     3661 cgccccggtt gccgtagata aggcccagcg tgtcggcgat ttccttgggg gcgtcggcgg
     3721 caaagggagc ggggagctct ttggtgctgg tgaccttggc gggaaccttc acctcgaaca
     3781 ggttggtgag cgtctggtcc accgccgcga tgttcttctt aacgacttcc tcgcccttgc
     3841 ggccgtaggt cttcttgatg gtgtacttga tctgctcgat ggcttccgcg cgcggcagca
     3901 cgccggacag ggcgaaaaag caggtctgca tgatcgtgtt gatgcgcgag cccatgccgg
     3961 tggccttggc gaccgtgagg gcatcgatga catagaactt gagcttcttc ttgatcaagg
     4021 tttcctgaac gggccggggc agcgtgtccc agatttcatt ctggccgtaa tgcgagttga
     4081 gcaggaaggt gccgccgggc accaggtcct tgagcatgtc atagcgatcc aggaagatcg
     4141 gctgatggca cgcgatgaag ttggccttgc tcaccaggta gatcgagcga atcggcttcg
     4201 ggccgaagcg caagtgcgag atggtgaccg cgcccgcctt cttcgagtca tacacgaagt
     4261 agccctgcgc gtagttgtcg gtgttctcgc caatgatctt gatcgaattc ttattggcgc
     4321 cgaccgtgcc gtctgagccc agcccgtaga acagcgcgcg gacgaccttg tccgactcgg
     4381 tggagaactc cgcatcgcaa gcgaggctgg tgtgggtgac gtcatcgtca atgcccacgg
     4441 taaagtggtt cttcggatgg gcggtctgga ggttgtcgaa cacggccttg accatcgccg
     4501 gcgtgaaatc cttcgaggcc agaccatagc ggccgccgag caccttcggc atggacttga
     4561 gcttgcccca gccggcggcc aggccctcgt tgagggcggc catgatatcc aggtagagcg
     4621 gctcgcccaa agcgtccgat tccttcgtcc gatccagcac cgcaagggct ttgacgctgg
     4681 ccggcagcgc ctccatgaag cgcttcacgc agaacggccg gtaaagccgc accttcagca
     4741 cgccgacctt gccgccgcgc tcgttgaggt aatccgccgt ctcatccgcc gtctcgcagc
     4801 ccgagcccat gaggatcacc acgcgctccg cgttcgggtc gccgacgtat tggaacggct
     4861 tgtattggcg gccggtcagg gcggcaaact ggtccatcgc cttgacggta atgtcggcgc
     4921 acttggcgta gaacggattg cacgcctcgc gcgcctggaa gaacacgtcc ggattctgcg
     4981 ccgtgccgcg cagcaccggg cggtcgggat tcaacgcccg ctgccggtgc gcaatcacga
     5041 actcctcgtt aatcatcttg cggatgatgt cctccgtgac cagctcgatc ttcgacacct
     5101 catgcgacgt gcggaacccg tcgaagaagt gcaggaacgg aatccggctc tccagcgtcg
     5161 ccgcctgcgc gatcagggcg aagtccatcg cttcctgcac gctcgccgaa ctgagcatgg
     5221 caaaccccgt cgtgcgcgcc agcatcacgt cgctatggtc gccaaagatg gacaacgcgt
     5281 gcgtcgcgag cgtgcgtgcg gtgatatgga acacggtggg cgtcagttcc cccgcgatct
     5341 tcaacatatt gggcaccttc agcagtaagc cctgggaggc cgtgaacgtg caggttagcg
     5401 cgcccgtttg gagcgccccg tgcaccgcgc cggcggctcc gccttcgctc tgcatttcca
     5461 ctacggaggg gatggtgccc cagaggttct tgatcccttc ggaggcccat tggtcgcacc
     5521 actcgcccat gtttgaggag ggggtgatgg gataaatccc catcacttcg ttgcaccggt
     5581 aggccacata agccaccgct tcattgccgt caattgtcgt ataggttttg ttcattttac
     5641 taaagcatta agccgttaaa gacgtcgctc tgttcgcggt cactgtttgc tttccgcggc
     5701 tgtctcccgc catgaattgc gaatcctcat ccagcgactc tgtaatgtgc ccagacgcag
     5761 ggtaggggac ttcaccagct ttggcaaaag ttcaccctat tttgcgccgt ctgaactcca
     5821 tcagcccgac acaaacaaag cccctcacga aacggctctt cgtttccccg cccgtcgtaa
     5881 gggtgacggg ccccggctgc acaaaggaag tagatttacc gacttgttcc caacggggag
     5941 gcggcaggtt tgcgctctgc ttccggaatc gccgcgggag cggcggaaga tgataaaggc
     6001 ctgagttgcg cctgtcactg ctgcctgacg agttttgtat cgaatcgaag cggacctgca
     6061 accgcactgc tcgacttgct gactccttta acccaccctt ctgccagcaa ctcgctgaca
     6121 atttcgaaga ctctctcatc cgagacacgg ggacagaacg acctttcgca tggctgcgtc
     6181 cgttggtggg gaagcgcgcc aaattcgcaa gcaggggctt tcagttcaag ggttcccaaa
     6241 cgcccggcct gaagcacctg aacgaccgaa tgggcggagc cgcaggcgtt ggtctgatcc
     6301 caaactctct gcccatcggc caaagccagt ctgactatgc aatacccaaa ccgttgcgca
     6361 gcaaagacta aatccgacca aagctcttct accgaatcgt gccgaccgcc ttctagcgcc
     6421 agccaatgcg tcagagtcag ggcgtagtgt atttcctgcc gcatatcgag tgaattaccc
     6481 aatacccggc ccaccgcgaa ccaatcccgg ctgaattgta gtct
//
LOCUS       DATCGS010000384         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_626564, whole genome shotgun sequence.
ACCESSION   DATCGS010000384 DATCGS010000000
VERSION     DATCGS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626564"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..>2595
                     /locus_tag="VIL39_07890"
     CDS             268..>2595
                     /locus_tag="VIL39_07890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY5481384.1"
                     /translation="MQPSPGPTEHGQRKEFAALMRGALLLDLEVSHQGNILKLGAVCG
                     EASLARSRGNSLASALAELAALGTNAKLVLGHNLVRFDLPILRQAAPRHPLLKLPIID
                     TLILSPICFPENPYHRLVKDYKLVRESINDPVADARQAATLFADEFQALDGLRQKDPR
                     LFEVLHFLLATPDPEADQLSRGMAMLFAALGGTRPPKDRVLAACRELMPQWGCASVPV
                     DESLVQTQSRRLALAYVLTWLRVAGSNSVLPPWVRLEHPLTGELIKRLREVPCHSVDC
                     AYCRRVHNSQEQLRTFFAFDAFRPTPQSSTGASLQQEIVEAGMRNDSLLAILPTGGGK
                     SLCFQLPALVRNYRRGVLTIVLSPLQALMKDQVDGLVRRTGTPFAAALYGLLTPPERS
                     DVLRRVRLGDVALLYVSPEQLRNRSFAEAISQREIGCWVFDEAHCLSKWGHDFRPDYL
                     YAGRFIREFSKKQGSEVPPIACFTATAKQDVREEILAYFKKEAGRDLAFYAGGVERDN
                     LRFEVQTVADSDKLARVHELLSERLPEGRPGSAIVFCATRNETELTAEFLRTHGWKVA
                     HFHAGLKPPEKKRTQDEFLSGDTRVICATNAFGMGIDKEDVRLVIHADTPGSLENYLQ
                     EAGRAGRDGMKAECVLLYAEEDCERQFRMEAFSELSRRDIAQILRSLRKATRGDKEEL
                     VITAGEILRDEELQIDIDLQDRMADTKVRTAISWLERANLLRRDENVTKVFQARPLVQ
                     NLDEARAKMSGLNLSPTEQSLWLAILREIFNSPPTE"
BASE COUNT          486 a          857 c          790 g          462 t
ORIGIN      
        1 gacggaacca ccaggcctta gccgggacga tgcagttgga agtggggagc acgcccgccc
       61 cgggcgttgc cgcccggcgc cctcgccggg tggctgccac gctgggagag tcattgaacg
      121 acgagtacgt tcgggcggcc tggaaagtga ctggcgaggg cgccagccac ggcacccggg
      181 gcgggcgcgc tcccctgcca ccgaatcgtt acggcttaga agtttggaca actccgcagc
      241 cagttggtta tgctaagcgg gaaacgcatg cagccgagcc ccggaccaac tgagcacggg
      301 caacgaaagg agttcgctgc cctgatgcgg ggtgcgttgc tgttggacct cgaggtctcc
      361 catcaaggca acattctcaa gctcggagcc gtgtgcggtg aagcatcgct cgcgcgctct
      421 cggggaaaca gcttggcctc cgcgctagcc gagttagccg cgctgggcac aaacgccaag
      481 ttagttcttg gccacaatct cgtgcggttc gatctgccga tcctccgtca agcggcccct
      541 cgccacccgc tgcttaagtt gcccatcatt gacaccctca tactgtcccc catctgcttc
      601 ccggaaaacc cttaccaccg actggtgaag gactataagc tcgtccgcga aagcataaac
      661 gatccggtcg cggatgcccg ccaggccgcc actctctttg cggatgagtt ccaagcactc
      721 gacggcctcc ggcagaagga cccgcgcctc ttcgaggtcc tgcactttct cctggcgact
      781 ccagacccgg aggccgatca actctcgcgc gggatggcga tgctgttcgc tgcgctgggt
      841 ggcacccggc ccccaaagga tcgtgtgctc gctgcatgtc gcgaactgat gccccaatgg
      901 ggttgcgcca gcgtcccagt tgacgaatcc ctggtgcaga cccaatcccg ccgcctggcg
      961 ctggcttacg tgctcacctg gctgcgggtg gccggctcga actcagtgct cccgccctgg
     1021 gttcgtttgg agcacccgct caccggcgag ctgatcaaac gcctgcgcga ggtcccttgc
     1081 cactcggtgg actgcgccta ttgccggcgc gtccacaact cccaggaaca attgcggacc
     1141 ttcttcgcgt tcgatgcctt tcgtccgacc ccgcagagca gcactggagc cagcctgcag
     1201 caggaaatcg tggaagccgg catgcgcaac gattccctgc tggcgattct gcccacgggc
     1261 ggcggcaagt cgctttgctt tcaactgccg gccctggtgc gcaattatcg ccgtggcgtc
     1321 ctgaccattg tcctctctcc cttgcaagcc ctgatgaaag accaggtgga cggcctggtc
     1381 cggcgcacgg gaacgccctt tgcggctgcc ctctatggcc tgctcacccc acccgagcgg
     1441 agcgacgtgc tgcgccgagt tcgacttggc gacgtagcgc tgctctatgt ctcgcccgag
     1501 cagctccgca atcgctcgtt tgccgaggcc atctcccagc gagagatcgg ctgctgggtg
     1561 ttcgatgagg cgcactgtct ctccaaatgg ggccacgact tccggccgga ctatctttac
     1621 gccggccggt tcatcagaga gttctcgaag aagcaaggaa gcgaggtccc gcccatcgcc
     1681 tgttttacgg ccaccgccaa gcaggacgtg cgggaggaaa tcctcgccta cttcaagaag
     1741 gaagctggac gggacctggc tttttatgcc ggcggagtcg agcgagacaa cctgcgcttc
     1801 gaggtgcaga ccgtcgcgga ttcagacaag ttagcgcggg tgcacgagct gctctcggag
     1861 cgcttgcccg agggacgccc tggcagcgct atcgtcttct gcgccacccg gaatgaaacg
     1921 gaactcaccg cggaattcct gcgcacgcac ggctggaaag tagcccactt ccatgccggc
     1981 ctgaagcctc cggagaagaa gcgcacccag gacgaattcc tcagcgggga cacccgcgtg
     2041 atctgcgcga cgaacgcctt cggcatgggc attgacaagg aggacgtccg cttggtcatc
     2101 cacgccgata cgccgggctc actggagaac tacctacagg aagcgggtcg cgctggacgc
     2161 gacgggatga aggccgaatg cgtgctgctc tacgccgagg aagactgcga gcggcagttc
     2221 cgaatggaag ccttctccga gctcagccgg cgcgacattg cccaaatcct caggagcctc
     2281 agaaaagcca cgcgcggaga caaggaggag cttgtcatta ctgccggcga gatccttcgc
     2341 gacgaagagc tccaaattga tattgatctc caggaccgca tggccgacac caaagtgagg
     2401 acggccattt cctggctgga gcgtgcgaac cttctccgcc gcgacgagaa tgttacgaag
     2461 gtgttccaag cgcgccccct ggtccagaac ctggacgagg cccgggccaa aatgtcgggc
     2521 ctgaacctgt cccccaccga gcagtcgctg tggctcgcga ttctccgcga gatattcaac
     2581 agcccgccga ccgag
//
LOCUS       DATCGS010000385         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_182067, whole genome shotgun sequence.
ACCESSION   DATCGS010000385 DATCGS010000000
VERSION     DATCGS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182067"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1338
                     /locus_tag="VIL39_07895"
     CDS             112..1338
                     /locus_tag="VIL39_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481385.1"
                     /translation="MNDLCSSLQFVAVLDGMPLLPLGAYLYPGISTGTAPGHRFDDNN
                     SVAHSSVMGYKACMATDNPEFVDPAEDCPWCSPREIFARYGYAPLPPSKLDDRQLPGR
                     LWEWLYAAAARRFFICSTNHLSDRELYTLLWEKWLDEPTADIPPEAQTNTTTIISEFN
                     ARGMTHEEIYLRYYADEGDQALWRSSDPELVFPPHQDPPYDRDRFLPVPPIPLEAHAG
                     WLPGDDDLPADDESDPLGLACIDGEIAAANTESPGLDPSDPALEAQLRASEPDNWTPP
                     AQELAKESVALLPPAEITEETLTPILWVLLHNLALRGFYVLHTDHLSDVELYTGLWKH
                     GLREPAHLPGRSLRGGWFHDFLGSWGEDDMRLWLRYYASDEERAKHAADWPKDTMPLK
                     EKPPFNRDWRLPKGPF"
     gene            1831..2016
                     /locus_tag="VIL39_07900"
     CDS             1831..2016
                     /locus_tag="VIL39_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481386.1"
                     /translation="MNATAIVGGKVVKFVLPPALAKAHQRNAGRIMTDQEAVDFVETK
                     RRRLALRRAKPYLESRS"
     gene            2000..2407
                     /locus_tag="VIL39_07905"
     CDS             2000..2407
                     /locus_tag="VIL39_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5481387.1"
                     /translation="MKAVLDTCILKLASLPNPNNPAALIVKLCWDGLLECWASPAMQE
                     EYSAVLADQPELLALVQDRFHVCYPLVELDCVKHEPDNRFVECALAIDADYLVTVNIA
                     RGHFDQPAYGRSRVVTPGTFVNLPAVQPMLRKL"
BASE COUNT          479 a          788 c          665 g          476 t
ORIGIN      
        1 gctcatgata cgtcaccgga tttgtgaccg ggactactaa ggaagctgtc gcaacgcctg
       61 attccatcga gaacggcgct aaaccgggtt ttgctctcta aggcccagga aatgaatgac
      121 ttgtgctcca gcctccagtt cgtcgctgtt ctcgatggaa tgcccctgct ccccttgggt
      181 gcctacctat accccggcat ctccaccggg actgcaccgg ggcaccggtt cgacgacaac
      241 aatagcgtgg cccactcctc cgtgatgggt tacaaagcct gcatggctac cgataatcca
      301 gagtttgttg atcccgccga agattgtccc tggtgctcgc cgagagagat ctttgcccgt
      361 tatggctacg cgcctttgcc gccatccaag ctcgacgacc ggcaattgcc tggccggctt
      421 tgggaatggc tctacgctgc cgccgcccgt cgctttttca tttgcagcac caaccacctg
      481 agcgaccgcg agctttacac gctgctgtgg gagaaatggc tcgacgagcc gaccgccgac
      541 atcccacccg aagcccagac caacaccacc acgatcatct cggagttcaa tgcccgtggg
      601 atgacgcacg aagaaatcta tcttcgctac tatgcggatg aaggcgacca ggcgctgtgg
      661 cgttccagcg atcctgagtt ggttttcccg ccgcaccagg acccgcctta cgaccgtgac
      721 cgatttctcc cagtaccgcc gattccgctc gaagcccacg ccggctggct gccgggcgac
      781 gacgatttgc cggcagacga cgaatccgat ccactcggcc ttgcctgtat cgatggcgaa
      841 atcgctgccg ccaatactga atccccaggt ctcgacccat ccgatcctgc cctcgaggcc
      901 cagcttcgcg ccagtgagcc ggacaactgg acgccaccgg cgcaggaatt ggccaaggaa
      961 agcgttgccc tcctgccgcc tgctgaaatc accgaggaaa cgctgacccc catactctgg
     1021 gtactgttgc acaatctagc cttgcgcggc ttctacgtcc tgcacacgga tcacttaagc
     1081 gatgtcgagc tttacaccgg cctctggaaa cacggccttc gcgaacccgc gcatttgccc
     1141 ggacgaagct tgcgcggcgg ttggtttcac gatttcctcg gcagttgggg ggaggacgat
     1201 atgcgactct ggctgcggta ttacgccagc gacgaagagc gcgccaaaca tgcggcagat
     1261 tggccgaagg acaccatgcc cctgaaggaa aagccgccct tcaatcgcga ttggcgtctg
     1321 ccgaaagggc cgttttgatt caccgcccgg ttgcagcctc ctgagtctcg cgctgcagcc
     1381 agtcccgcac caactcggga agcttgcaat gctcgcaaat tgcgttggaa tcggggtcgg
     1441 aagagcttgt tcaaactggc gggcgtgccg aacaaaggac gaagtgagaa taagctgttc
     1501 tgacccagac tggactgggc gctgttgagt ccttccattt cgcagtgggt atggtctggc
     1561 tcggccaaaa gtgtcaatag tgagaacttg ctgcgaactg tggaaggaaa aacccgaccc
     1621 gaatgtccgg gcctggttct tctcctctga ttggttcctg ccagttcccg tcatcgcaca
     1681 aattcaagag ggcgccaagg cctcgcccag cgctgcccgg aggattgaaa tatcccgcaa
     1741 gttcgacgcc tccctgcatg cagccttggt gtcgctctcg ccctgaaaag tcggcttgaa
     1801 cgccagcacc ggccgcagta gcttcccccc atgaacgcaa cagcaatcgt gggcggcaaa
     1861 gtggtgaagt tcgtgcttcc gcccgcgctg gccaaggcgc accagcgcaa tgccggccgc
     1921 atcatgaccg accaggaggc cgtggacttc gtggaaacga aacgccgccg actggccctg
     1981 cgccgggcca agccctacct tgaaagccgt tcttgacacc tgcatcctga agctggcctc
     2041 cctgcccaat cccaacaacc ccgctgctct catcgtcaag ttgtgctggg acggactcct
     2101 ggaatgttgg gcctcgccgg ccatgcagga ggaatactcg gcggtgctgg ccgaccaacc
     2161 ggaactgttg gccctggtcc aagaccgctt ccatgtctgc tacccgctgg tcgaactcga
     2221 ctgcgtcaag cacgaacccg acaatcgctt cgtggaatgt gcgctcgcca ttgacgccga
     2281 ctatctcgtg accgtcaaca ttgcccgtgg gcactttgat cagcccgcct acggacgctc
     2341 ccgcgtcgtc accccgggaa ccttcgtgaa ccttccggcg gtgcagccga tgctgcgcaa
     2401 actctgaa
//
LOCUS       DATCGS010000386         6056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_82221, whole genome shotgun sequence.
ACCESSION   DATCGS010000386 DATCGS010000000
VERSION     DATCGS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82221"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VIL39_07910"
     CDS             complement(<1..650)
                     /locus_tag="VIL39_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481388.1"
                     /translation="MSTLVVPGAQCVMGAAPAVPDDAFPACIQFSPVPDGKGWKGEKP
                     PISAEVQRNTLLNLIGHGFSVLYYPIGGLSDAQNQEVLASAQALGMRVNYMTGGFEMF
                     DREHPPAISVYSPRYAEEVSKRVQAGLAPMKAIQRVYSVFPFQDEPFHAGPGAFDYSS
                     DAKAEFSRRYGYALPASLDSVRNDPKQWLDLLNFQSETFRDGWRQVYRIVKAFDPRP"
     gene            complement(886..1653)
                     /locus_tag="VIL39_07915"
     CDS             complement(886..1653)
                     /locus_tag="VIL39_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481389.1"
                     /translation="MKKLIIRLLITLVVVAILAVLAVGLFLDGAIKRGVEAFGPKLAK
                     VDIKLQSVSLSLFSGAGTIKGLVVGNPEGFKTPSAISIGEATLALKPGSLLSDKIVIK
                     TINVQAPEIMFETDLRYDNLNKILSNLQEATGGGTKEPAKGAKPARKLQVDEFVITGG
                     KIHVSVTGLAQGTATVPLPDIRLQNLGAGPDGITSTELTKLVLEAIEKGAAQAASGAV
                     TDIGKGALYITKELGSTTGSNTLQKATKGLGGLFKKK"
     gene            complement(1722..2129)
                     /locus_tag="VIL39_07920"
     CDS             complement(1722..2129)
                     /locus_tag="VIL39_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481390.1"
                     /translation="MNESKVNSLGSGQNNGALIYLVDDEPMLLELASVILAPLGYRIE
                     TFRAAKAALRAFKAAEAPPALIITDYEMDAMTGLDLMEACRQIQPRQKFLLVSGTVGP
                     DIFSAGPVRPVRFLAKPYQSKQLIDLVEAVLAD"
     gene            complement(2245..2652)
                     /locus_tag="VIL39_07925"
     CDS             complement(2245..2652)
                     /locus_tag="VIL39_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481391.1"
                     /translation="MSNQKLDQLVDQVENYIECWKQFNNFVNLARAKKFSQEDDNQFL
                     EIKSILVQELELILASIEVSSPTREDIHALITEAPSLRYLSEMNEGALRALENQWHRI
                     YIGWHAILGQLKVRQRGEQSKSAIGSVFRKKKR"
     gene            complement(2665..2973)
                     /locus_tag="VIL39_07930"
     CDS             complement(2665..2973)
                     /locus_tag="VIL39_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481392.1"
                     /translation="MGLLSFFSKSAPTLLRLPSGSFTVDREGSVLMSTLTSNFPASLV
                     KDIAQQMLATFGEAAKAQLPLAELTINYPSLTISARELRGGAIVFLSPKPPRSPASQS
                     "
     gene            complement(2961..4022)
                     /locus_tag="VIL39_07935"
     CDS             complement(2961..4022)
                     /locus_tag="VIL39_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481393.1"
                     /translation="MDTRPKILLADDDPDLLDMYREVLAQLPSRPEIRTAASGARVIA
                     MLEAEPFQLLICDLKMPKMDGLQVLAIARRKYPHLRTVAMTAELDEQFRSRAYALGVD
                     LFWHKPGSEQEIKMFLECIESLLGRETEAGFRGVQSKSLMDIIQLECISQSSSVLRIT
                     NGMLSGKIWILSGELVDAEADDLRGEAAFQRILSWRAGNFEMLPADPSRPRTIFNSYN
                     ALLLESAQALDESRGGVTAEGAAQPAASPLARLSQVEGVEFVLAMKPSQGGYEVARGL
                     ENPERMAAWARHSLERFRLLGDRLGAGPLQQIEGFGPQRHVALACQDETEFCVGWKHS
                     LAPEQVRAMMKKLLALWGS"
     gene            4457..>6056
                     /locus_tag="VIL39_07940"
     CDS             4457..>6056
                     /locus_tag="VIL39_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY5481394.1"
                     /translation="MFGYVKNLLRKWVEKPDDNQSPTVVSAAPAPSRRTVMAPPPEDA
                     PAHNNDSHQNGRGIELPLQPILGGLPLELQPRLRHPDAGALTISIPLDKILTQLSRGA
                     VKITFGELRQAAPDLFTPEDDRDRVMVSLPLGEILSRLNPALITRRRVQKQVEVPDEI
                     CSPFDQRNQSLIFSVGPTHPEAAPVSAPTPRQTAVSAPAPVAPPARGGVAFTPSPAPP
                     SAAPLSTLAMSSATRALPNLRNSHSPLPPVPTPQMAPTAPAPPSPGPSIDPHPLLVSL
                     TSLAEGWPEAVRQELVQLNLVDAKVALPAEIVERALKQGRIAFTWKTLRSWIKPAPLP
                     AVSVHDSLMLELPLRIVAPPFLARQREVGKLQKKVTVGDDIPNLFFGFPRPEASRPVM
                     ASAASSAAAKPPDTNFYVWDDTSDRVCPRGGETKPDPSPGTKFVAKYATPNEVVSRAA
                     TLDGVAGALIALPDGLMVASHLAPDLNGDTLAAFLPQIFGKVSQCTKELRMGELNNVN
                     FTVGNVPWKIFRVNAIFFAAFGRMG"
BASE COUNT         1214 a         1880 c         1755 g         1207 t
ORIGIN      
        1 gggcgcggat cgaacgcctt cacgattcga tagacctggc gccagccatc gcggaaggtc
       61 tcggactgga agttgagcag atccagccat tgttttgggt cgttccgcac ggaatcgagg
      121 ctggccggca gggcatagcc atagcggcgg ctgaattccg ccttggcgtc gctgctataa
      181 tcgaaggcgc cgggaccggc atggaacggt tcatcctgga aagggaatac actatagacc
      241 cgctggatcg ctttcatggg cgccaggcct gcctgaacgc gcttgctgac ctcctccgca
      301 taacgaggcg agtacacgct gatcgcggga ggatgctccc gatcaaacat ctcgaaaccg
      361 cccgtcatgt agttgactct catgcccagc gcctgggccg aagccaggac ctcctgattc
      421 tgcgcgtcgg agaggccgcc aatggggtag tagagcacgc taaaaccgtg gccgatcagg
      481 ttcagcagcg tattccgctg aacctcggcg gaaatgggcg gcttctcgcc cttccagccc
      541 ttgccgtcag gaaccgggga gaactggata caggctggga aggcatcgtc tgggaccgct
      601 ggggcggcac ccatcacgca ttgcgcaccg ggcacaacca gggtgctcat gatggcaagg
      661 ctggaggcac cggccctgga ccgctgcagc attcgcagca acgaaaagcc cctgagccag
      721 tgcgaaatat tcatcctgca atagtgtgtt ctcctggcta atcagtaaag cgtgggaaag
      781 aggaacgccc gatgtgggcg tgcaagctct gcgcctcgtg ctggtaccga caagctgcac
      841 ccgcatgtgg gtgtaaacca tcgcccagtc cctgtcttcg cctcactatt tcttcttgaa
      901 caggccgccg aggcccttgg tggccttctg gagagtgttg ctgcctgtgg tactgcccag
      961 ctctttcgtg atatagagcg ctcctttgcc gatgtccgtc accgcaccgg aagcggcctg
     1021 cgcggcgccc ttctcgatag cttccagcac cagtttcgtc agctcggtgg aggtaatgcc
     1081 gtcgggaccc gcgcccagat tctgaagacg aatgtccggc aaggggaccg tcgctgttcc
     1141 ctgggccaag ccggtcacgc tgacatgaat cttcccgccg gtgatgacga actcatccac
     1201 ctggagtttc ctggccggct tcgccccctt ggcgggctcc ttcgtccctc cgccggtagc
     1261 ctcctgcagg ttcgatagaa tcttgttcag gttgtcatat ctgagatcgg tctcaaacat
     1321 gatctccgga gcctgaacat tgatggtctt gatgacgatc ttgtccgaaa gcagcgaacc
     1381 gggtttaagg gcgagggtgg cctcgccgat actgatggcc gagggcgtct tgaatccctc
     1441 gggattgccc accaccagac ctttgatagt gcctgccccg gaaaacaaag acaggctgac
     1501 ggactgcagc ttgatatcta ctttggccag tttcggtcca aatgcttcca ccccgcgctt
     1561 gatggcccca tccaagaaca ggccgaccgc cagaacggca agaatggcca ccacgacgag
     1621 ggtgatgagc agacgaataa taagcttctt catagctata cccgattgct gttgctcggc
     1681 agcgttgccg attgtcattt ttaatgagcg ccgaattctg gctaatccgc cagcaccgct
     1741 tccaccaggt cgatgagttg tttcgactga tacggcttgg ccagaaaacg aacgggccta
     1801 accggcccag cgctgaagat gtccggcccc accgtgccgc taaccaggag aaacttttgc
     1861 cgcggctgga tttgccggca cgcctccatc aagtccaggc cggtcatcgc gtccatctcg
     1921 taatcggtga tgatgagggc gggcggcgct tcggcggcct tgaatgcccg gagagccgcc
     1981 ttcgcggcgc gaaaggtttc gatcctgtag ccgagaggcg caaggatcac cgaggccagt
     2041 tccagcagca ttggctcgtc gtccaccaag taaatcaacg ctccgttgtt ttgcccggat
     2101 ccaagactgt ttaccttcga ctcattcatt tccttatgcc ctagtctgaa taaacacccg
     2161 ccggagccgg atggcaagcg gttgttgcgt tattctgcct ccaaaagagg ctcacagata
     2221 cggcgaaact ccttgatcgt ggcgctacct ctttttcttt cggaacacgg agccaatggc
     2281 ggacttggat tgctcgcccc gctggcggac cttcaattgg ccgaggatgg cgtgccagcc
     2341 aatatagatt ctgtgccatt ggttttccaa ggcgcgcaag gcgccttcat tcatctcact
     2401 caggtagcgc agcgacgggg cttcagtgat cagggcgtga atgtcttccc gggtcggaga
     2461 agagacctcg atggacgcca ggatcagttc caattcctga acgaggatgc tcttgatctc
     2521 caggaactgg ttatcgtctt cctggctgaa tttcttggcg cgtgccaggt tgacgaagtt
     2581 attgaactgt ttccagcact cgatgtagtt ttccacctgg tcaacgagtt ggtccaattt
     2641 ttgattgctc ataggctcga gagtttagct ttggctcgcg ggtgaacgag ggggcttggg
     2701 agaaagaaac acaatcgcgc cgccccgcag ctcgcgggcg gagatcgtca agcttgggta
     2761 gttgatggta agctcggcca ggggcaattg ggccttggcg gcttccccaa aggtggcgag
     2821 catctgctgg gcaatgtcct tgaccaggct ggcgggaaag ttcgaagtca aagtgctcat
     2881 gagcacactg ccctcccggt caaccgtgaa gctgccggaa ggcaggcgca gcaatgtggg
     2941 cgccgatttg gaaaagaaac tcaggagccc catagtgcta aaagcttctt catcatggcc
     3001 cggacctgct ccggggccag agaatgtttc cagccgacac aaaactcagt ttcatcctgg
     3061 caggctagcg cgacgtgacg ctgcggccca aagccctcaa tctgttggag ggggcccgct
     3121 cccaggcggt cgcccagcaa acggaatcgt tccaggctgt gacgagccca cgcggccatg
     3181 cgctccgggt tttccagacc gcgagcgacc tcgtatcccc cttggctggg cttcatcgcc
     3241 agcacaaatt ccacgccttc gacctgcgac aggcgcgcca gcggcgaagc agcgggttgc
     3301 gccgcgcctt cagcagtcac cccaccccgc gattcatcca gcgcctgggc tgattcgagg
     3361 agcagcgcat tgtaggaatt aaagatggtg cgcggccggc tgggatcagc cggcagcatc
     3421 tcaaagttgc cggccctcca ggagagtatt ctttggaagg cggcttcacc gcgcaggtca
     3481 tccgcttcgg cgtcaaccag ctcgccgctt agaatccaga tcttcccgct gagcatgccg
     3541 ttggtgatgc gcagcaccga ggaactctgc gaaatacatt ccaactggat gatgtccatg
     3601 aggctcttgc tttgcacccc gcggaagccg gcctcggttt cacgcccgag caacgactcg
     3661 atgcactcca ggaacatctt gatctcttgt tcgctgccgg gcttgtgcca gaagaggtcc
     3721 acgcccagag cataggcgcg agagcggaac tgctcgtcca gctccgccgt catcgccact
     3781 gtgcgcaggt gcggatactt gcggcgggcg atcgccagga cctgcaagcc gtccattttc
     3841 ggcatcttca agtcacaaat cagcaactgg aacggctcag cctccagcat ggctataacg
     3901 cgcgcgccgg aggcggcggt acggatctcg ggccggctgg gcaactgcgc cagcacttcc
     3961 cgatacatat ccagcaaatc cggatcgtca tccgccagca gaattttagg gcgcgtgtcc
     4021 atttcttcca acgccaaccg ctaactcggt atcaagacaa attcaagcat gaaacaccac
     4081 atgtagtttc ctgcgttcag tagcaaggat tattccccga agacgacgat tacgaagttt
     4141 gttggattca gcgtgcccag cctgtgattt caggggatgt ggcgttccga agcgcaaact
     4201 ccaaaagccg ccagaaggcc gggaaagggc agcaacagga caaccagtcc agtgatggga
     4261 cagccaagga ggccacgctg ccccgccggg gcattgggcc tgaaggcaga gcccggactg
     4321 cggcaccgat tgtgcctgtc gtctcagagg gagccgcccc agcccaggcc ccacggcccg
     4381 gaaaggagtt tgtagccaca atggcatagt tcctgcgcca gagccacgta gaagtagttc
     4441 aacaggaata aaccagatgt ttggttacgt aaagaatctt ctgcgaaagt gggttgagaa
     4501 acccgacgac aaccagtctc cgacggtggt cagcgccgcg cctgccccct cgcgccggac
     4561 cgtgatggct ccgcctcccg aagatgcgcc ggcgcacaac aacgactcgc atcaaaatgg
     4621 cagggggatc gaactgccct tgcagcccat tctcggtggg ctgccgctgg aattgcagcc
     4681 ccggctcagg cacccggatg ctggcgcgct gaccatttcc attccactcg ataaaattct
     4741 gacgcaactc tcgcgcggcg ccgtaaagat tacctttggc gaattacggc aggccgcgcc
     4801 cgaccttttc accccggaag acgatcgcga ccgggtcatg gtttcgctgc cattgggaga
     4861 gatacttagc cggctcaacc ccgccttgat cacacgccga cgtgttcaaa agcaggtaga
     4921 agttccagat gagatctgca gtccgttcga ccagcgcaat cagagcctga tcttttcggt
     4981 tggcccgacc caccccgaag cagccccggt ttcggcccca accccgcgtc agactgcggt
     5041 ttccgcacca gcaccggtcg caccgcctgc ccgcggcggg gttgctttca cgccctcgcc
     5101 cgcgccgccc agtgcggccc cgctcagcac gctcgcgatg agttctgcta cgcgcgcatt
     5161 gcccaacctg aggaacagcc attctccctt accgccggtt ccgacacctc aaatggcccc
     5221 gacggccccc gctcctccct cgccaggacc ctcgatcgat ccccatccat tgctggtcag
     5281 cttgacttct ctggcggagg gctggccgga agctgtccgg caggaactcg tgcaactgaa
     5341 cctggtggat gccaaggtgg cgttgccggc cgagatcgtc gagcgggcgc tcaagcaggg
     5401 ccggatcgcc ttcacctgga aaaccctgcg ctcgtggatc aagcccgcgc cgctaccggc
     5461 ggtctccgtc catgacagtc tcatgctcga actgccgttg aggattgtcg cgcctccgtt
     5521 cttggcccgg cagagagaag tcggcaaatt gcagaagaag gtcacggtag gcgacgacat
     5581 tcccaacttg ttctttggtt ttccgcgacc ggaggcgagc cgccccgtga tggcttccgc
     5641 cgccagttcc gcggcggcca agccgccgga tacgaatttc tacgtttggg acgacacgtc
     5701 cgatcgcgtc tgcccgcggg gaggcgaaac caaacccgac ccctcccccg gaaccaagtt
     5761 cgtcgccaag tacgctacgc ccaacgaagt cgtctcacgg gcggctacgc tggatggggt
     5821 ggccggggcg ctcattgctt tgcccgacgg cttgatggtg gccagccatc ttgccccaga
     5881 cctcaatggc gacacgctgg cggcctttct gccccaaatc ttcggcaaag tcagtcaatg
     5941 caccaaggaa ctccggatgg gcgaactcaa taatgtcaac ttcaccgtag gcaatgttcc
     6001 ttggaagatc ttccgagtga acgcgatctt cttcgcggcc ttcggccgca tgggcg
//
LOCUS       DATCGS010000387         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_676747, whole genome shotgun sequence.
ACCESSION   DATCGS010000387 DATCGS010000000
VERSION     DATCGS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676747"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VIL39_07945"
     CDS             <1..669
                     /locus_tag="VIL39_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5481395.1"
                     /translation="SDRKEVSDGLYAWLAAGDQGRNNPTNRGTIAAWAWGYHRCVDYL
                     VTEPSVDSHRIAALGHSRNGKTALLAAAFDERIAIAFPHQAGCGGSAPSRGKTGESVK
                     AINDHFPHWFNAQFKQFNDAPERLPFDQNCLVALCAPRPVLFSAGEDDQWANPAGQFQ
                     VLQAADGVYRFLGTEGLAAKEIPRQRNLVTSPLGYYVREGKHSMTPDDWATFMNFADS
                     QYRK"
     gene            710..2035
                     /gene="argJ"
                     /locus_tag="VIL39_07950"
     CDS             710..2035
                     /gene="argJ"
                     /locus_tag="VIL39_07950"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY5481396.1"
                     /translation="MKAKLTEIPGSILAPQGFRAAGVFSDIKRLGTGKGSDKGKKRDL
                     ALIVSEAPGTVAGMFTTNQACAAPVKVCVERVKKGRAQAIVVNSGNANACTGKQGLQD
                     ALEMVRFTEQALAFPQGSVLVGSTGRIGVTMPMDNVRAGIIEAAALLGSTAGHAAQAA
                     EAIMTSDTRSKQVAVEFKVGGQVVRIGGICKGAGMIQPGMSATGKRPSAAPLHATMLC
                     FITSDAAIEPKALQAALREAAANSFNRITVDGDMSTNDTVLVLANGLARNRDFGLGTL
                     DFGLFQAALTHVCLTLAKMIVRDGEGVSRVVTVRVNGAKTFADADAAARAVANSPLVK
                     TSWHGGDPNWGRIIAAVGYSPATIIEEKVDIGYSAPGSRKILWSLKRGQPTSATFKAL
                     CAAVAPKEFDLHINLNLGQAGALMYAADLTEEYVEFNKGDVNDPTSLGG"
     gene            2049..2924
                     /gene="argB"
                     /locus_tag="VIL39_07955"
     CDS             2049..2924
                     /gene="argB"
                     /locus_tag="VIL39_07955"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5481397.1"
                     /translation="MQNLIAKTATLLEALPYIQKFSGATFVIKYGGSFMDSPDPAVRN
                     GVARDLVFLEAVEINPVVVHGGGKAITRAMEKAGLKANFIQGQRVTDEATVRIADDVL
                     SREINPEVVAAINAMGGLAKGFAGPDIFRCRKAHVKGLDGQAIDLGYVGEVTGVNLAP
                     LLDCIEKGITPVISPTARGEDGKIYNCNADVAAAQAAIALKAKRLVFMSDVPGLLQNP
                     KSPDTVISRLMISEVDSLKQRGVVDKGMIPKVDSAVAAIKAGVEKVSLVDGRVPHAVL
                     LEIFTDEGIGTEVVG"
BASE COUNT          663 a         1044 c          984 g          558 t
ORIGIN      
        1 tccgatcgca aagaggtcag cgatggcctt tacgcctggc tggcagccgg ggatcagggc
       61 aggaacaacc cgaccaaccg gggcacgatt gccgcatggg cctggggcta ccatcgctgc
      121 gtggactacc ttgtcaccga accgagcgtg gactcgcacc gcatcgcggc gctgggccac
      181 tcgcgcaacg gcaagacagc gctactcgcg gcggcattcg acgagcgcat cgcgatcgca
      241 ttcccgcacc aggcgggctg cggcggctcc gcgccaagcc ggggaaagac cggcgaatcc
      301 gtgaaagcca ttaacgacca tttcccacat tggttcaacg ctcagttcaa gcagttcaac
      361 gatgcgccag agcggctgcc attcgaccag aattgtctgg tggccctttg cgccccgcga
      421 ccggtgctct tctccgccgg cgaagacgac caatgggcca acccggccgg ccagtttcag
      481 gtgctccagg ctgccgacgg agtttatcgc ttcctcggca ccgaaggatt ggccgcgaag
      541 gaaatcccgc gccagcgcaa cctcgtcacg agtcccctcg gctattatgt gcgcgagggc
      601 aagcattcaa tgacgccgga tgactgggcg acgtttatga actttgcgga cagccaatac
      661 cgaaagtgag cctgaaccca agactgtgac acagattctg actgctggca tgaaagctaa
      721 actaactgag atcccaggtt ccatccttgc gccgcaaggc ttccgcgccg ccggcgtctt
      781 ttccgacatc aaacgcctcg gcaccggcaa aggctcggac aaggggaaaa agcgcgacct
      841 ggctctcatt gtctctgagg cgcccggcac cgtggccggc atgttcacca ctaaccaagc
      901 ctgcgccgcg ccggttaaag tctgcgtcga acgcgtgaaa aaaggacgcg ctcaggcaat
      961 cgttgttaac tccggcaacg ccaatgcctg caccggcaag cagggtttac aggatgcgct
     1021 ggaaatggtg cgcttcacgg aacaagcgct cgccttcccg caaggctcgg tcctcgtcgg
     1081 gtccaccggc cgtattggcg tcacgatgcc gatggataat gtccgcgccg gcatcatcga
     1141 agccgcggcc ctgcttggct ccactgcggg tcatgccgcc caggccgccg aagccattat
     1201 gaccagcgat acgcggtcca aacaggtcgc cgtcgaattc aaggtgggcg gccaggtcgt
     1261 ccgcatcggc ggcatctgca aaggcgcggg catgattcag cccgggatga gcgccacggg
     1321 caagcggccc agtgccgcgc ccctccatgc gacgatgctg tgcttcatca cgagcgatgc
     1381 cgcaattgag cccaaggcgc tgcaggccgc gttgcgcgag gccgccgcca acagcttcaa
     1441 ccgcatcacc gtggacggcg acatgagcac gaacgacacg gtgctggtgc tggccaatgg
     1501 cctcgcccgc aaccgagact ttggacttgg gactttagac tttggactct tccaagccgc
     1561 cctgacccac gtctgcctta cgctggccaa gatgatcgtg cgcgatggcg aaggcgtgtc
     1621 gcgggtcgtc accgtccggg tcaacggcgc gaaaaccttc gccgatgccg atgccgcggc
     1681 gcgcgccgtg gcgaacagcc cgctggtcaa gaccagttgg cacggcggcg atccaaattg
     1741 gggccgcatc atcgctgcgg tgggctacag cccggcgact attattgagg aaaaggtgga
     1801 catcggctat agcgcgcccg gcagccgcaa gatcctctgg agtctcaagc gcggccagcc
     1861 gaccagtgcc acgttcaaag cgctctgcgc ggccgtcgcc ccaaaggagt ttgacctgca
     1921 tatcaatctc aacctcggcc aggccggcgc gctcatgtat gccgccgacc tcaccgaaga
     1981 gtatgtggag ttcaacaaag gtgatgtcaa cgatcccact tcgctgggag ggtgaccttt
     2041 tatttcacat gcaaaacctc attgccaaga ccgccacatt gctggaagcg ttgccttaca
     2101 tccagaaatt cagcggcgcc accttcgtca tcaaatacgg cggcagcttc atggactcgc
     2161 cggacccggc tgtccgcaac ggcgtcgcgc gtgacctggt cttcctggag gccgtggaga
     2221 tcaaccccgt ggtcgtccac ggcggcggca aggccatcac ccgcgcaatg gagaaagccg
     2281 gcctcaaggc caatttcatc cagggccagc gtgtcaccga cgaagccacg gtgcggatcg
     2341 ccgacgacgt gctctcccgc gagatcaacc ctgaagtggt tgccgcgatc aacgccatgg
     2401 gcggcctcgc caaagggttc gccggtcccg atattttccg ctgccgcaaa gcccatgtca
     2461 aagggctgga cggccaagcc atcgacctcg gctacgtggg cgaggttacc ggcgtgaatt
     2521 tggccccgct gctcgattgc atcgagaagg gcatcacccc cgtcatcagc ccgaccgccc
     2581 gcggcgagga tggcaagatt tacaactgca acgccgacgt ggccgcggcg caagcggcaa
     2641 tcgcgctgaa agccaaacgc ctggtcttca tgagcgatgt cccggggctg ctgcagaacc
     2701 cgaaaagccc ggacacggtt atttcccgcc tcatgatcag cgaggtggac agcctgaaac
     2761 agcgcggggt ggtggacaaa gggatgattc ccaaagtgga cagcgccgtg gccgccatca
     2821 aggccggcgt ggagaaagtc tcgctcgtgg atggccgcgt gccccacgcc gtgctgctgg
     2881 agattttcac cgacgaaggc attggcaccg aagtagtagg gtagccggcg agtccccttg
     2941 caaatggagt agagccatga gccgcgagcg gcgggccttt tcccaggaat gaaaacaagc
     3001 ccttccccgc agcgactccg gccagtgaag ctgcttgggc aaggcactgc gcaggcgccc
     3061 tttcatcttt tcgcgtggct aaaagaaatt gaactgctag gcgcggaatg cgactttctc
     3121 agatgtgacg aaccaggctg cttatgtgct gcgcgagact accggaactt ccggtgtcat
     3181 gcaaactgcc caaacagccg ttgccacctt ggcggcccat gatattccgc acctcattgt
     3241 tggcggggt
//
LOCUS       DATCGS010000388         4603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_132607, whole genome shotgun sequence.
ACCESSION   DATCGS010000388 DATCGS010000000
VERSION     DATCGS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132607"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VIL39_07960"
     CDS             <1..167
                     /locus_tag="VIL39_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY5481398.1"
                     /translation="FVSSELTELMAVCDRMLVMSAGRIAAEFLPGEWTQEKITHAAFS
                     GYRDRQKAVS"
     gene            complement(162..1976)
                     /locus_tag="VIL39_07965"
     CDS             complement(162..1976)
                     /locus_tag="VIL39_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY5481399.1"
                     /translation="MITSAPAPSGDLSPVALAASPEGKNLYVACAAAKQVLIFNPGQR
                     QVAKRLALPGEPSGLVLSSDGTRLFVTCAGPASQVCVIDTVSGKMLQTLAAGHTALSP
                     VLSPDGNTLFVCNRFNNSVSVFDLRRGQEQGRIQVAREPVSAALTHDGRFLLVANHLP
                     HGRSDVPYVAATVSVIDVAQRKVVKELHLPNGSINLRQICVSPDGKYACLAQTVGRFQ
                     VPVTQIARGWVNTSALTLIDLTSLELLNTVLLDEPDRGAANPWAVAWSGDGRWLCVTH
                     AGTYELSVIDFPALLKKLAALPATPPPPSVKDNYLSSRSAADVPNDLSFLYGLRQRVQ
                     LNGRGPRALVIAGNRAFVVGYFSDSLDVVDLSAAKPVAEAVALNPGHQMTTVRRGEMY
                     FNDATICFQGWQSCASCHDEDARVDGLNWDLLNDGIGNPKDTKSLVLSHQTPPVMSLG
                     IRATAEIAVRNGIANSLATSLPEEVPAAMDEWIKSLKPAPSPHLVKARLSEAARRGEK
                     LFKSPDTGCVNCHDTELFTDLHSYNVGTQNPFDKEDKEFDTPTLRELWRTAPYLHDGS
                     AATVRDVLTTRNPKDEHGKTSQLTAQQIDDLVEYVLSL"
     gene            2392..3090
                     /locus_tag="VIL39_07970"
     CDS             2392..3090
                     /locus_tag="VIL39_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY5481400.1"
                     /translation="MKHSGKARKAAFKLILISLIVLVVVWALGAFTAAAVAIITAVAV
                     AITPVLIVVWILFALFTLYFFRDPRPRVPAGPNLVVAPAHGKVDVVGMGTEPDFMGGE
                     CQRISIFLSVFDVHVQNAPVSGKVAWFKYTTGQFLNALKAESAIYNENVLLGFEANEP
                     RGQKVGVRLIAGVIARRIVPFVQQGDEVVRGDRISLIQFGSRADVYLPRNARIMVKVD
                     DRVVAGETVLAVFE"
     gene            3113..3970
                     /gene="pssA"
                     /locus_tag="VIL39_07975"
     CDS             3113..3970
                     /gene="pssA"
                     /locus_tag="VIL39_07975"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY5481401.1"
                     /translation="METPSPKPPTNGTDEPKLKIYFLPNLLTAANLFCGFVALTKIVE
                     ANVAGGDFQQIKVALAFILLACIFDLFDGRVARMGGVESPFGREFDSLADLISFGAAP
                     AFLVHRVVLKDVFEGHSEWGWFIASVYLLCGAFRLARFNCVSAMPHAPSKDFQGFPIP
                     SAAGLVASLTLFMIWWYEKEFAKSNWRYCLPVLLLFLSWMMVSNVRYPSFKTLDLRAT
                     RTFTKTLIAILFLGSILIFREKILVFVLPVFFTAYLIYGFIRPRISHRMRHEIEEEEE
                     EEEGPVAPG"
     gene            3977..>4603
                     /locus_tag="VIL39_07980"
     CDS             3977..>4603
                     /locus_tag="VIL39_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5481402.1"
                     /translation="MPELPTILVAGLTGLISGVLLSIPVGPINLTILNEGARRGFKWA
                     ALIGLGATTMEVIYCFVAFTGFASFFTRGYVKAAMELFSFVFMLFIGTKFLFAKSVQA
                     KPVPLGAAADKIEVRLEEWLHPHSAFMIGLVRVMGNVGVLVFWIILAANFISREWVTP
                     DWPGKLACVAGVAVSTGGWFTGLSWVVSLGHGKWSEKTLLRMEHFSGAG"
BASE COUNT          792 a         1313 c         1566 g          932 t
ORIGIN      
        1 ttttcgtgtc cagcgagctg acggagttaa tggcggtgtg cgaccgcatg ctcgtgatgt
       61 ccgccggacg cattgccgcg gaattcctgc ccggcgaatg gactcaggag aagatcacgc
      121 acgccgcctt cagcggctac cgcgaccggc agaaggccgt ttcataggga gagtacatac
      181 tcgaccaggt catcaatctg ctgcgcggtg agctgagacg tcttgccatg ctcatccttc
      241 gggttgcggg tggtcagcac gtcgcggacc gtggcggctg agccgtcatg caggtagggc
      301 gctgtgcgcc aaagctcgcg cagcgtcggc gtgtcaaact ccttatcttc cttgtcgaag
      361 gggttctgcg tgccgacgtt gtaagagtgc agatccgtga acagctccgt gtcgtggcaa
      421 ttgacgcagc cggtgtcggg gctcttgaac aacttctcgc cgcgccgcgc cgcctctgac
      481 agccgtgcct tgaccaggtg cgggctcgga gccggcttga gcgatttgat ccactcgtcc
      541 atcgccgccg gcacttcctc gggcaacgag gtggcaagag agttcgcgat gccgttccgg
      601 acggcgattt cggccgtggc ccggatgccc aggctcatga ccggcggcgt ctggtgggat
      661 agcaccaggc tcttggtgtc tttcggattg ccgataccgt cgttgagcaa gtcccagtta
      721 aggccatcca ctcgcgcgtc ctcgtcgtgg caactggcgc agctctgcca gccctggaag
      781 cagatcgtcg cgtcgttgaa atacatctcg ccgcgccgaa ccgtcgtcat ctgatgcccg
      841 ggattcaacg ccacggcctc ggccacgggt ttcgcggcgg agaggtccac cacgtccagg
      901 ctgtcagaga agtagccaac cacaaaggcc cggttgccgg cgatgaccaa cgcgcgcggg
      961 ccgcggccat tgagctgcac ccgctggcgc aggccataga ggaaggacaa atcgttgggc
     1021 acgtccgcag cggagcgcga gctgaggtag ttatccttca ccgacggggg cgggggcgtg
     1081 gcgggcaagg cggccagctt cttcagcagc gccgggaagt caatgacgct caattcgtat
     1141 gtgcctgcgt gggtgacaca gagccaccgg ccgtcgccgg accacgctac ggcccacgga
     1201 ttggccgcgc cacgatccgg ctcgtccagc agcaccgtgt tcagcaattc cagggaggtc
     1261 agatcaatga gtgtgagggc gctggtgttg acccaaccgc gcgcgatttg ggtcaccggg
     1321 acctggaacc ggcccacggt ctgcgccagg caggcgtatt tgccatccgg ggagacgcag
     1381 atctggcgca aattgatgct gccgttcggc aggtgcagtt ctttgactac cttgcgctgg
     1441 gctacgtcaa tgacgctgac ggtcgccgct acataaggga cgtccgaacg gccgtgtggc
     1501 aggtggttgg ccaccagcag aaagcgccca tcgtgcgtca gggcggcgga cacaggctcg
     1561 cgcgccacct gaatgcggcc ctgctcctgg ccccggcgca ggtcgaacac cgagaccgag
     1621 ttgttgaaac ggttgcagac gaagagcgta ttcccgtcgg ggcttagcac agggctaagg
     1681 gcggtatggc cggcagccaa cgtctgcagc attttgccgg aaacagtatc aataacgcag
     1741 acctggctgg cgggtcccgc acaggtcaca aatagccgcg tgccgtcgga cgacagcacc
     1801 aatcccgagg gctcgcctgg caacgccagc cgtttagcaa cctggcgctg acccgggttg
     1861 aagatcagca cctgtttggc ggcggcacag gcgacgtaga ggttcttgcc ctcgggcgaa
     1921 gccgccaggg cgaccggcga caggtcgcct gacggggcgg gggcgctggt gatcacgggg
     1981 tcaagggctg gagaccggga gcaggctggc aggaatggga gcagggcgag aaggggtagg
     2041 aggattgcca atggccaatg gcagattgca aattgagagt tgccggggcg gggtggagac
     2101 gcgtggacca tgcaccgcct cggcattggg ttcggggtcc aatcatcgcc ggaggggctg
     2161 gttgctcttg agcggttgat catttcctgc actgcaaatc ggtcataccg cagagtgtga
     2221 atctgcgccg cgccgggtca agcctcgtta ggcgttgagg ctctttcggc tacgcgggtg
     2281 ggcgcgcgcg gcaatccaca tttccgaccg ccgcgaggaa gttggaagcc agaactctgc
     2341 gcgcaacttt gccctttgcc tggcacccct taggccttac cttctgactg aatgaaacac
     2401 tcgggcaagg ccaggaaggc agctttcaag ctgattctga tttcgctgat cgtgctggtc
     2461 gtggtgtggg cacttggcgc tttcaccgcg gcagcggtcg ctattattac ggcggtggcg
     2521 gtggcgatta cgccggtgct aatcgtggtc tggatcctgt tcgcgctatt cacgctctac
     2581 ttcttccggg acccgagacc gcgggtgcca gccggcccca acctcgtggt ggctccggca
     2641 catggcaagg tagatgtggt cggaatgggg acggagccgg acttcatggg cggggagtgc
     2701 cagcggattt ctatctttct ctcggtcttc gacgttcacg tgcagaacgc gccggtcagc
     2761 gggaaagtcg cctggttcaa atacaccacc ggccagttcc tcaacgcgct gaaggccgaa
     2821 tcggctattt ataacgagaa cgtgctgctc ggcttcgagg cgaacgagcc gcggggacag
     2881 aaggtcggcg tgcggctgat cgccggggtg atcgcgcggc gcatcgtgcc cttcgtccag
     2941 cagggcgacg aggtcgtccg cggtgatcgc atcagcctga tccagttcgg ctcgcgggct
     3001 gacgtgtatc tgccgcgcaa cgccaggatt atggtgaagg tggatgaccg tgtcgtcgcg
     3061 ggggaaaccg tgctggcggt gtttgagtga ttctgaaatg agcgaaccgg ttatggaaac
     3121 tccatccccc aagcctccaa ccaacggcac ggacgagccg aagttgaaga tttatttcct
     3181 cccgaacctg ctgacagcgg cgaacctgtt ctgcgggttt gtggcgctga ccaagatcgt
     3241 cgaggccaat gtcgccggcg gtgattttca gcagatcaaa gtggcgttgg cgttcatatt
     3301 gctggcgtgc atcttcgacc tgttcgacgg gcgagtcgcg cggatgggcg gcgtggaaag
     3361 cccgtttggc cgcgagtttg attcgctggc cgacctgatc tcgttcgggg cggcgccagc
     3421 atttctggtg catcgcgtcg tgctcaagga cgtgttcgag gggcactcgg agtggggctg
     3481 gttcattgcc tcggtctatc tgctgtgcgg cgcctttcgc ctggcgcggt tcaattgcgt
     3541 gtcggcgatg ccccatgcgc cctcgaagga ttttcagggt ttcccgattc cctcggcggc
     3601 agggttggtc gcctcgctca ccctgttcat gatctggtgg tacgagaagg aattcgcgaa
     3661 gagcaactgg cggtactgcc tgccggtcct cctgctgttc ctgtcctgga tgatggtcag
     3721 caacgtcagg tatccgagct tcaagacgct ggatttgcgg gcgacccgga ccttcacgaa
     3781 gacgctgata gcgatcctgt tcctcggcag catcctgatc ttccgggaga agatcctcgt
     3841 gttcgtgctg ccggtgttct ttacggcgta tctgatctac gggtttattc ggccgcggat
     3901 ttctcacagg atgcggcatg agatcgaaga ggaggaggag gaggaagagg ggccagtggc
     3961 gcccggttga ccgcacatgc ccgagctgcc gactattttg gttgccggct tgacaggcct
     4021 gatcagcggc gtgttgctct ccattccggt ggggccgatc aacctgacga tcctgaacga
     4081 gggggcgcgg cgcggattca aatgggcagc gctcatcggc ctgggcgcga cgaccatgga
     4141 ggtcatttac tgcttcgtcg ccttcaccgg ttttgcgtcc ttcttcacca ggggctacgt
     4201 caaggcggcg atggagttgt tcagcttcgt cttcatgttg ttcatcggga ccaagttcct
     4261 gttcgccaag tcggtgcagg cgaagccggt gcccctgggc gcggcggcgg acaagatcga
     4321 ggtgcggctc gaagaatggc tgcatccgca ttcggcgttc atgatcgggc tggtgcgggt
     4381 gatgggcaac gtgggggtgc tggtgttctg gattatcctg gcggccaact ttatctcgcg
     4441 cgaatgggta acgcccgatt ggccgggcaa gctcgcctgt gtagcgggcg tggcggtgag
     4501 cacgggcgga tggtttaccg ggctgagttg ggtggtttca ctcggccatg gaaaatggag
     4561 cgagaaaacg ttgctgcgga tggagcactt ctccggcgcg ggc
//
LOCUS       DATCGS010000389         2556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_428269, whole genome shotgun sequence.
ACCESSION   DATCGS010000389 DATCGS010000000
VERSION     DATCGS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428269"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..>2556)
                     /locus_tag="VIL39_07985"
     CDS             complement(145..>2556)
                     /locus_tag="VIL39_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY5481403.1"
                     /translation="VKVLSKMGISTIQSYWGAQIFEAIGLKQSFVEKYFTGTPSRIEG
                     VGIEVIAQEVLLRHQRAFPDRPVNGHLLDAGGQYQWRDDGEYHRFNPQTIHKLQRAVR
                     TGSYKIFKEYSALVNEQGRHWCTLRGLLDFKNVKPVPIEEVESVECIMKRFKTGAMSY
                     GSISKEAHETLAIAMNRIGGKSNTGEGGEDPERYVPLPNGDSKNSAIKQVASARFGVT
                     SLYLVNAKELQIKMAQGAKPGEGGQLPGRKVYPWIAKVRHSTPGVGLISPPPHHDIYS
                     IEDLAELIHDLKNANHHARISVKLVSEVGVGTVAAGVAKAHADVVLISGYDGGTGASP
                     QTGIKHAGIPWELGLAETHQTLVLNNLRSRIIVEADGQLKTGRDVIIAALLGAEEFGF
                     ATGPLVAMGCIMMRVCHLNTCPVGVATQDPELRKRFAGQPEHVVNFMRMIAEEVRELM
                     AQLGFRQMNKMIGRADRLEVKKAVDHWKARGLDFSQILYQVEVPTDVGRFCQIPQEHG
                     LERALDNTVLRKLCAPAIERKEKVLAFLPIRNVHRVVGTMVGSEVTRRWGVQGLPDDT
                     IEIHFTGSAGQSFGAFMPRGMTFFLEGDSNDGLGKGLSGGKLIVFPPAHSTFVPEENI
                     IIGNVALYGATSGEAYIRGMAGERFAVRNSGAEAVVESVGDHGCEYMTGGRVVVLGMT
                     GRNFAAGMSGGVAYVLDEAGDFARRCNPQMVGLEKLEDADEIELVWKMVQRHQAYTQS
                     ALAARLLANWLQIVPKFVKVMPKDYKRVLESLKRMEGSGLSGEQAIMAAFEENAHDAA
                     RIGGG"
BASE COUNT          453 a          820 c          770 g          513 t
ORIGIN      
        1 gcgggccgcc tgctcacgca gcttgcccgg ctccatgtgc tggtggaatt cgttccaatc
       61 gcggatgcgc tcgaccgccg agcggtccag cggcaactca cgcaagtatt cgataaaacc
      121 agtaggtttg cccatacttc taatttaccc tccgcctatc cgtgcggcgt cgtgggcgtt
      181 ttcttcgaag gccgccatga tggcttgctc gccgcttaag ccggatccct ccatgcgctt
      241 caaggactcc aatacgcgtt tgtaatcctt cggcatgacc ttgacgaact tgggtacaat
      301 ctgcagccag ttcgccagca gcctggccgc caaggcgctc tgcgtgtaag cctggtggcg
      361 ttggaccatc ttccacacca actcgatttc gtccgcgtcc tccagtttct ccaggccgac
      421 catctggggg ttgcagcggc gcgcgaaatc gcccgcctcg tccagcacgt aggcgacacc
      481 gcccgacatg ccggccgcaa aattacggcc ggtcatcccg agcacgacca cgcggccgcc
      541 ggtcatgtat tcgcagccgt gatctccgac cgattccacc acggcctcgg cgccgctgtt
      601 gcgcacggcg aagcgttcgc cagccatgcc acggatatac gcctcaccgc tggtggcccc
      661 gtagagagcc acattgccga tgatgatgtt ctcctccgga acgaaggtcg agtgcgccgg
      721 cggaaagaca atgagtttgc cgccggacag tcccttgccc aggccgtcgt tagaatcgcc
      781 ctccaggaag aaagtcatgc ccctgggcat gaatgcgcca aagctctgcc cggcactacc
      841 ggtgaaatgg atctcgatgg tatcatcagg caacccctgg acaccccagc gccgggtgac
      901 ttcgctgcca accatagtgc cgacgacccg gtgaacgttg cggatgggca ggaaggccag
      961 caccttctcc ttgcgctcaa tggcgggggc gcagagcttg cgcaggacgg tgttgtccag
     1021 cgcccgttcg agaccatgct cctgcggaat ctggcagaaa cggcccacgt cggtcggcac
     1081 ttcgacctgg tacagaatct ggctgaagtc cagaccccgg gctttccagt gatccaccgc
     1141 cttcttgact tcgagacggt cggcccggcc gatcatcttg ttcatctggc ggaacccaag
     1201 ctgcgccatg agctcgcgca cttcctcagc gatcatgcgc atgaagttga cgacgtgctc
     1261 cggctggccg gcgaagcgct tgcgcagctc cgggtcctga gtggcgacgc cgaccgggca
     1321 ggtgttgagg tggcagacgc gcatcatgat gcagcccatc gccaccagcg gcccggtggc
     1381 aaacccgaat tcctcggccc caagcagggc ggcgatgatg acgtcgcggc cggtcttcaa
     1441 ttggccgtcg gcctcgacaa taatgcgcga gcggaggttg ttgagcacga gggtctggtg
     1501 cgtctcagcc aacccaagct cccaggggat gcccgcatgc ttgatgccgg tttgcgggga
     1561 ggcgcccgtc ccgccatcgt acccgctgat gagcacgacg tcggcgtgcg ctttggcgac
     1621 gcccgccgcg acggtgccaa cgccaacctc ggacacgagc ttaacgctga tgcgcgcgtg
     1681 atggttcgcg ttcttcaagt cgtggattaa ctcggccaga tcctcaatag agtaaatgtc
     1741 atggtgtggg ggtggcgaga tcagccccac gcctggcgtg gagtggcgga ctttggcgat
     1801 ccacggatag acttttcggc cgggcaactg gccaccttca cccggcttgg cgccctgggc
     1861 cattttgatc tgaagctctt tggcgttgac cagatagagg ctggtgacgc cgaaccgggc
     1921 ggaagcaacc tgcttgatgg cgctgttctt cgagtccccg ttcggcaacg ggacatagcg
     1981 ctccggatct tcgccgcctt caccagtatt gctcttgccg ccgatgcggt tcatggcgat
     2041 ggcgagcgtc tcgtgcgcct ccttgctgat ggacccgtag ctcatggcgc ccgtcttgaa
     2101 gcgcttcatg atgcactcga ctgactcgac ctcctcgatg ggaacgggct tgacattctt
     2161 gaagtcgagc aacccgcgca gcgtgcacca gtggcggccc tgctcgttga ccagtgcaga
     2221 atactctttg aaaatcttgt agctgccggt gcgcaccgcc cgctgcaact tgtggatcgt
     2281 ctgcggatta aaccggtggt actcgccgtc atcgcgccat tggtattggc cgccggcgtc
     2341 gagcagatgg ccgttgaccg ggcggtccgg gaaggcgcgc tggtgtcgga ggaggacttc
     2401 ctgcgcaata acctcaatcc ccacgccttc gatgcgggag ggcgtgcccg tgaagtactt
     2461 ctctacaaag gactgcttca agccgatcgc ttcgaatatc tgcgcgcccc aatagctttg
     2521 gatggtggag atgcccatct tcgagagcac cttcac
//
LOCUS       DATCGS010000390         3141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_627421, whole genome shotgun sequence.
ACCESSION   DATCGS010000390 DATCGS010000000
VERSION     DATCGS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627421"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VIL39_07990"
     CDS             <1..386
                     /locus_tag="VIL39_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481404.1"
                     /translation="PLAKAARQLNGRAMQTGRSRGGDVFLADLGGNYLGRTCPWKECG
                     PGIRLSCDALHCGKRHYLHDDLRAIKLQPALIQAPPIELWPAVVARVEVPTDVEELLL
                     KPIRAGTPNLGQQQPSAKPGQTSSH"
     gene            complement(387..614)
                     /locus_tag="VIL39_07995"
     CDS             complement(387..614)
                     /locus_tag="VIL39_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2837 family protein"
                     /protein_id="HEY5481405.1"
                     /translation="MPNLTVILLLTAMVHCLDPVSALVTDQALRGSRPGSAVSHIVVW
                     QVAGRFAGTLLAQFLFVPAALLVVRIAHLLT"
     gene            complement(618..1874)
                     /gene="amrS"
                     /locus_tag="VIL39_08000"
     CDS             complement(618..1874)
                     /gene="amrS"
                     /locus_tag="VIL39_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5481406.1"
                     /translation="MPLSRRAAIGLGAAGLAGIAGAASLFEFFSKWRASEAPADIFKG
                     DAPKGELWELWRKRGWVKEASHYLKLGLNVQCKVCPNNCLLAPGDRSHCRNKVNYDGT
                     LYTLVYANPCTFHVDPVEKKPLFHFLPGSRTFSLATSGCVFRCLNCQNWDISQKKPEE
                     TKDPRGAELRMRPPLPSGFTSGEMARLSLFPSDAAAVAEALGCPSLSYTYTEPTAFYE
                     YTYDTCKAARERKLKNIVVSCGSIEDRPARDLYRLVDAAHVDLKGFDQDIYTKLNSGK
                     LQPILNTLKTLRGLGVWFEIINLVVPTYTDNPDTIRRMCAWIAKELGPDQPLHFSRFH
                     PQHKLEYLAPTPVETLVKARAAARSEGLRYVYIGNVPGLEGAETTWCPNCKKPVVERD
                     IFAVTALNLANGKCRSCGTRIAGVWS"
     gene            complement(1946..2209)
                     /locus_tag="VIL39_08005"
     CDS             complement(1946..2209)
                     /locus_tag="VIL39_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481407.1"
                     /translation="MNKFNFILLKIVRWSGWPLLPLVLLFLLTGYIMDGRYGFNRVLD
                     EKSALTFHRMLHLPLIVLILAHSVPATYLAMQRWGWIKPRAEP"
     gene            complement(2287..3063)
                     /locus_tag="VIL39_08010"
     CDS             complement(2287..3063)
                     /locus_tag="VIL39_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481408.1"
                     /translation="MIRRSRDLDGMLTADRMADLANATAQPAAMNRDFSPILYYYHLR
                     HWMSQFNLRFGLLQAGLLGLLGYYLFRLRGSAFVLFASGFAASALEVVLLLAFQVLCG
                     SVYHQVGVIVTVFMAGLAVGALMANRLSNCGRGSLSRLALAIAAYASLLPLLLPLLNR
                     MGGSAASLALVKVIIGLFTLVLAVLVGMQFPLANRLEFDGTVAGASRLYTADFVGACL
                     GALLASTLLIPLIGVVGMCLLTAALNVLGALAGRRRAAAA"
BASE COUNT          646 a          968 c         1007 g          520 t
ORIGIN      
        1 cgcctctggc gaaggcggcc cggcagttga atggtcgggc tatgcaaaca ggccggagcc
       61 gaggcggcga tgtgttcctg gcggacctgg gcgggaatta cctgggccgg acatgtccgt
      121 ggaaggaatg cggtcccgga atccgcctga gttgcgatgc gctgcactgt ggcaagcggc
      181 attacctcca cgacgatctg cgtgccatca agctccagcc agcgctaatt caggccccac
      241 cgattgaact ctggccggca gtggtggccc gggtggaagt gccgaccgat gtcgaggagc
      301 tcctactgaa gccaatccgg gcgggcacgc cgaacctggg tcagcagcag ccttcagcaa
      361 agccagggca gacgagctct cattgactac gtcaataggt gcgcaatcct caccaccaac
      421 agcgccgccg ggacgaatag gaactgggca agcagggtgc cggcaaatcg ccccgccacc
      481 tgccagacca caatatggct caccgctgaa cccggccggc tgccgcgcaa cgcctggtcg
      541 gtgaccagcg ccgagaccgg gtccaggcag tgaaccatag ccgtcagcag cagaatgacc
      601 gttagattcg gcatcaacta gctccaaacc ccggcgatcc gggtgccgca ggaacggcac
      661 ttgccattgg ccaggttcag ggcggtaacc gcgaagatgt cccgttcgac gaccggcttc
      721 ttgcagttgg gacaccaagt cgtctccgcg ccttcgaggc cggggacgtt gccgatatac
      781 acgtagcgca agccctcgct gcgggcggcg gcgcgcgcct tcacgagcgt ctcgacgggc
      841 gtgggcgcga ggtactccag cttgtgctgc ggatggaagc gcgagaaatg gagcggctga
      901 tccggcccca gctccttggc gatccacgca cacattcggc ggatggtatc cgggttgtcg
      961 gtgtaggtcg gcacgacgag gttgatgatc tcgaaccaga cacccagccc cctgagtgtc
     1021 ttgagggtat tgaggatggg ctggagcttg cccgaattca gcttggtgta gatgtcctgg
     1081 tcgaagcctt tgaggtccac gtgcgccgcg tcaacgagcc ggtaaaggtc gcgcgccggg
     1141 cggtcctcga tggagccgca actgaccacg atgttcttca gcttgcgttc gcgcgctgcc
     1201 ttgcacgtgt cgtaggtgta ttcgtaaaag gccgttggct ctgtgtaagt ataggaaagc
     1261 gatggacagc ccagcgcctc cgccaccgcg gcagcatcag atgggaacag gctcaggcgc
     1321 gccatctcgc ccgaagtgaa accagagggc agcggcggcc gcatccgcaa ctccgcccct
     1381 cgcgggtcct tggtctcctc gggcttcttc tgggagatgt cccagttctg gcagttcagg
     1441 cagcgaaaga cgcagccgga ggtcgcgagc gaaaaggtgc ggctgccggg caggaaatga
     1501 aacagcggct tcttctcaac cgggtcaaca tggaaggtgc agggattggc atagaccagc
     1561 gtatagaggg tgccgtcgta attaaccttg ttccggcaat ggctgcggtc accgggtgcg
     1621 agcagacaat tgttcgggca caccttgcat tggacgttaa ggcccaactt gaggtagtgc
     1681 gaggcttcct tgacccagcc gcgcttgcgc catagttccc acagctcacc cttcggcgcg
     1741 tcccccttga agatgtcggc gggggcctcc gaggcgcgcc atttggagaa gaactcgaac
     1801 agcgacgccg ccccggcgat accggcgagg ccagccgcgc ccagcccaat agctgcacgg
     1861 cgagacaagg gcatattcgt ctgctttcgg ctttcggctt tcggccttcc ttcggccttc
     1921 ggccttcgga tttcggattt cccgctcacg gttctgcgcg gggcttgatc cagccccatc
     1981 gctgcatggc aagatacgtc gccggcacgc tatgcgccag tatgagaaca atcagcggca
     2041 gatggagcat gcggtgaaag gtgagcgccg acttctcgtc cagcacgcga ttgaaaccgt
     2101 agcggccatc catgatgtag ccggtcagca gaaacagcag gaccagcggc agcagcggcc
     2161 aaccgctcca ccggactatc ttgagaagga tgaaattgaa cttgttcatg cgacgagttt
     2221 gccaacccca ttgtctcacc ccgggcttat cgcgcgtcaa tggaatttga tgctcccctg
     2281 ccgcgctcag gctgcggccg ccctgcggcg gcccgccaac gctccgagga cgttgagcgc
     2341 cgcggtcaac agacacatgc caacgacgcc gattagcggg atcaacagcg tagaggccag
     2401 cagcgcaccc aggcatgcgc cgacgaaatc ggccgtgtaa agtcgggacg ccccagcaac
     2461 ggtgccgtcg aactctagcc ggttagccag gggaaactgc atcccgacca gcacggccaa
     2521 caccagcgtg aacaggccaa tgatcacttt gaccagcgcc agtgacgccg ccgagccgcc
     2581 cattcggttc agcagcggca agaggagcgg gagaagcgag gcgtaggcgg cgatggccag
     2641 ggcgagccgg gacaggctgc cacggccgca gttcgagagc cggttcgcca tgagagcacc
     2701 caccgccagg cccgccatga agaccgttac gatgacgccc acctggtgat aaaccgagcc
     2761 gcacaggacc tggaaagcca acagcaggac tacctccagg gcggaagccg caaagccgga
     2821 agcgaacagg acgaaagcgc tgccgcgcag gcggaagagg tagtagccaa gcaaccccag
     2881 caggccagcc tgcagcagac caaaacggag gttgaactga ctcatccagt gccggaggtg
     2941 atagtagtaa aggatgggac tgaagtccct gttcatcgcc gccggctggg cggtggcgtt
     3001 ggcgagatcc gccatccggt ccgccgttag catgccgtcc aggtcacggc tccggcggat
     3061 cattgcataa ggtgttgcag atgaagcgga tctaagattt ggcgggacaa ttcgcctcga
     3121 ggctgccaaa cttggcgcca t
//
LOCUS       DATCGS010000391         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_677446, whole genome shotgun sequence.
ACCESSION   DATCGS010000391 DATCGS010000000
VERSION     DATCGS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677446"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VIL39_08015"
     CDS             <1..985
                     /locus_tag="VIL39_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481409.1"
                     /translation="GSAHSSGAGSGSHSASSGGSAGQSHGTSVSQGSRSESSSGKSYS
                     SGSTSSDNSRHSYTSAKSYSSGAGHTFTSSTGSDSGVREPQPAHSVSPASSGPGLAFD
                     AAAAHARKEEASQAKFTQFKEAQLPPSATPGDAARSAAPSEAPRSAQGAPSYRVQPPP
                     LSARAGSSYRGVVYVPDAGTLSSRPVRIYNVFKPYYSRPVVIYRDPYNSLFWWWLLDR
                     SIDDRAGWAYHHRYDMDPARYQALVANDQQLETRVEQLEAQQAPRDPNYAPPGVDRDL
                     MYSDRHVEQLYSNRPTASGVFAFWLLAVPTALGVTGFFIWLIWFKRWQTAT"
     gene            1008..1247
                     /locus_tag="VIL39_08020"
     CDS             1008..1247
                     /locus_tag="VIL39_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY5481410.1"
                     /translation="MQTHLPDYLLTDLLATVAFGVVAILLIVLGYITFDKLTPKLNFS
                     DLLNKGNTAMAIVVGSFILGLCYVIGRVVAAILGG"
     gene            1310..1993
                     /locus_tag="VIL39_08025"
     CDS             1310..1993
                     /locus_tag="VIL39_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481411.1"
                     /translation="MEPENKGKTLWEMLQERLHGNSNGAGIAFANPLDLRVGSPVAVA
                     YANGSEYTDYNFSVQEMREYTRRIEAQEFRFTDYVLRGVNTKSPDADDTVAARLRVVP
                     NQAGAHDALLLRLYDEFAFAEDFLGVVKDTTGLFKVTDDKSGAETTFSRINDLRESYQ
                     AAVLVVSETTPDGKGATGKVAPAKVEYWDYWHDADIGGGKTAKEFIFVEMNSDTGWFQ
                     IWRGREFFS"
     gene            complement(1996..2412)
                     /locus_tag="VIL39_08030"
     CDS             complement(1996..2412)
                     /locus_tag="VIL39_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEY5481412.1"
                     /translation="MASTIEDTPITRKTRELCQMLLDEPGMQAIRQRIDTFMADEQTR
                     SQYDGLIAKGQALQEKQQQSQPLSGEEISEFEQQRDSLLKNPVARGFLDAQQELHQVR
                     ESIQKYVSKTIELGRLPSEEELDSGCRGDHSCGCCE"
     gene            3003..4184
                     /locus_tag="VIL39_08035"
     CDS             3003..4184
                     /locus_tag="VIL39_08035"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY5481413.1"
                     /translation="MPAKHQRKHAPQLIVGLMSGTSADGIDAVVAEIVGSGRQLRARV
                     LAHVHRPFSSAMRQRILRLCLHGTVAEICELNFVLGEQFARAALAVIRRAKRKPADIA
                     AIGSHGQTIHHLPNARTPSTLQIGEAAVIAERTGITTVADFRVRDMAAGGQGAPLVPY
                     ADWALFTDQTRPRIVQNIGGIGNLTFLPPRAELADVIAFDTGPGNMVMDALVMALSRG
                     RQTFDRDGRWAARGRVSEKLLAELMTHPFLRRRPPKTTGREEFGEVFVQRVLAAARRL
                     RLGPEDTIATATAFTAASIADAYQRFVFPKLKAGKRGALQIILGGGGARNPTLRRMLA
                     ERIGAGELLTHEDFGIANAAKEALAFAILAHETLLGKPGNVPSATGARRAVVLGKIVP
                     G"
     gene            4409..>4711
                     /locus_tag="VIL39_08040"
     CDS             4409..>4711
                     /locus_tag="VIL39_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY5481414.1"
                     /translation="MGRLNIKPDGSCKYDLVALGEIMLRLDPGEGRVRTAREFKVWEG
                     GGEYNVARAMKKCWGKRSSVVTALPKNDLGWLVEDFICQGGVDTSHIIWREFDGIGR"
BASE COUNT          859 a         1486 c         1506 g          860 t
ORIGIN      
        1 agggtccgcg cacagctcag gcgccggcag cgggagccac agcgccagct cgggcggcag
       61 cgccggccag agccacggga ccagcgtaag ccagggatcc cgctcagaaa gcagcagcgg
      121 caagagctac agttccggct ccacctcgag cgacaatagc cggcacagct atacttccgc
      181 caagagttac agttccggcg cgggccatac ttttacctcc agcaccggca gtgacagcgg
      241 tgtccgggaa ccccaacccg cgcacagcgt cagcccggct tcgagcggtc ccggccttgc
      301 attcgacgct gcggccgcac atgcccggaa ggaggaagct agccaggcga aattcacgca
      361 gttcaaggaa gcgcagcttc cgccgtccgc cacgccgggc gacgctgcgc ggtccgcggc
      421 gccgagcgag gcccctcgct ctgcacaggg cgcgccgtca taccgggtcc agccgccgcc
      481 gctttcggcg cgtgccggca gttcttaccg gggcgtggtt tatgtgccgg acgccggaac
      541 gctttccagc cggccggtgc ggatttacaa cgtgttcaag ccctactact cgcggcccgt
      601 ggtgatctac cgcgacccct acaacagctt gttctggtgg tggctgctgg accgctctat
      661 tgatgaccgg gccgggtggg cttatcatca ccgctacgac atggacccgg cgcggtacca
      721 ggccctggtg gccaacgacc agcaattgga gacgcgcgtc gaacaactgg aagcccaaca
      781 ggcgccgcgc gatccgaact atgcgccgcc cggcgtggac cgggatttga tgtattccga
      841 ccgtcacgta gagcagttgt atagcaatcg cccaacggcc tcgggagtgt ttgccttctg
      901 gctgctggcc gtcccgacgg cgctaggcgt gacgggcttc tttatttggc tgatttggtt
      961 caaacgctgg caaaccgcaa cctgattgaa acagagaaac acaccttatg cagacacacc
     1021 tccctgacta cctgctgact gacctcctcg ccactgtcgc ctttggcgtg gtggcgatcc
     1081 tcctcatcgt gctcggctac atcacgttcg acaaactcac gcccaagctc aacttcagcg
     1141 acctgctcaa caagggcaac accgccatgg ccatcgttgt cgggtccttc atcctgggcc
     1201 tgtgctacgt gatcggccgg gtggtggcgg ccattttggg aggctaggcg cacgcgttgg
     1261 cgccacccct tttgccgcac ccgtatcatc gtcatcctca ctcgcgcata tggaacctga
     1321 gaacaaaggc aagaccctct gggaaatgct ccaggagcgg ctccacggca acagcaacgg
     1381 cgccggcatt gcgttcgcca atccgctcga tctgcgggtc ggctcaccgg tcgccgtcgc
     1441 ctatgccaac ggctccgagt acaccgatta caacttctcg gtgcaggaga tgcgtgaata
     1501 cacccggcgc attgaggccc aggagtttcg gttcacggat tacgtcctgc gcggcgtcaa
     1561 caccaagtcc cccgacgccg atgacacagt ggccgcccgc ctgcgcgtcg tgcccaacca
     1621 ggccggggcg cacgatgcgc tcctgctgcg gctctacgat gagttcgcgt ttgccgagga
     1681 ctttcttggc gtggtcaagg acaccaccgg gctgttcaag gtgacggacg acaagtccgg
     1741 cgccgaaacg acattctcgc gcatcaacga cctgcgcgaa tcctaccagg cggcagtcct
     1801 ggtggtgtca gagacgacgc ccgatggcaa aggcgccacc ggcaaagttg cgccggccaa
     1861 agtcgagtat tgggactact ggcacgacgc ggacatcggc ggcggcaaaa ccgcgaagga
     1921 attcatcttc gtcgaaatga actccgacac gggctggttc cagatctggc gggggcggga
     1981 gttcttctcc tgaagctact cgcagcagcc gcagctatgg tcgccgcgac agccgctgtc
     2041 taactcttcc tccgaaggca ggcggcccag ttcgatcgtc ttgctgacgt atttctggat
     2101 ggattcccgc acctgatgca gctcttgctg cgcgtccagg aagccgcggg cgaccgggtt
     2161 cttcagcagc gagtcgcgct gctgctcgaa ttcggaaatc tcttcgccgc tcagcggctg
     2221 cgactgctgc tgcttctctt gcagcgcctg gcctttcgcg atcaggccgt cgtattggga
     2281 ccgggtttgc tcatccgcca taaaggtgtc gatgcgctgg cggatggcct gcatgcccgg
     2341 ctcatccaaa agcatttggc agagctcccg tgtcttccgg gtaatcggag tgtcttcaat
     2401 cgtcgatgcc atattcagta tgtattgctg ttcgttgaca cgggcactcg gccatttggc
     2461 gtgtcagcct cacaatacat catacaacca gcgcaaaagc aaaccattgt ctggccacgc
     2521 tagagcaatc gggacgcgcg ctcgcctgca tcaggcaaaa gaagcgcgtc atcggccggt
     2581 ggcggagcgt cggaaggagg atttggggga gaagttggtt tcgttcgaat catcgtccgg
     2641 cccggactgt cagtaccctt caggggctcg gtggagccgc cgtggcgccc cagagggcgc
     2701 caccggagaa gacgatgaac aataaaggaa caatagagca ccaatcgagg aacaatcgag
     2761 agcacctccc atgctcctgg cttgccggtg gcttgtgggg ggcttgcaca ggggtttggg
     2821 gtggcttgtc ggccgcaatc agctcccaag caggtgattt accccctgta tgccccctgt
     2881 ataccccttg tatgccccct gtattgaatt cgcttgttcc tggctttgcc ttggccttgc
     2941 ccagcctttg ccctacccaa ggcgacgctt gttcggtcgg gccggtttgg taggctcagt
     3001 ccgtgccagc taagcaccaa cgcaaacatg cgccgcagct catcgtgggc ctgatgtccg
     3061 ggacctcggc ggacggcatt gatgcggtgg tggcggaaat tgtggggagc ggacgccagc
     3121 ttcgtgcgcg cgtcctggcg cacgtgcatc ggcccttctc ctcggccatg cggcagcgca
     3181 tcctgcgctt gtgcctgcac gggacggtgg cggagatttg cgaactgaac tttgtgctgg
     3241 gggaacagtt tgcccgcgcg gcgctggccg tcatccgccg ggcgaaacgc aagcctgcgg
     3301 atatcgccgc catcggctcg cacgggcaga ccatccatca cttgccgaat gcgcgaacgc
     3361 cttcgacgtt gcagatcggc gaggcggccg tcatcgcgga gcggacgggc atcaccaccg
     3421 tggcggactt tcgcgtgcgc gacatggccg ccggcggcca gggggcgccg ctggtgccgt
     3481 atgcggattg ggcgctgttc accgaccaga cgcggcctcg catcgtgcag aatatcggcg
     3541 gcatcggcaa cctgacgttc ctgccgccgc gcgcagagtt ggccgacgtg atcgcgttcg
     3601 ataccgggcc gggcaacatg gtgatggatg cgctggtgat ggcgctgagc cggggcaggc
     3661 agacctttga ccgcgacggc cgctgggcgg cgcgcggccg cgtgtcagag aagctgctgg
     3721 cggaactgat gacccacccg ttcctgcgac gccgtccgcc caagaccacc gggcgcgaag
     3781 agttcggcga ggtgttcgtc caacgcgtgc tggcggcggc gcggcggctg cgcttgggcc
     3841 cggaggacac gattgccacg gcgacggcgt tcaccgccgc gagcattgcc gatgcttacc
     3901 agcgctttgt cttcccgaaa ctcaaggcag gtaagcgtgg cgccttgcag atcatcctgg
     3961 gtggtggcgg cgccaggaat ccgacactcc gtcgcatgtt agccgagcga attggcgcgg
     4021 gcgagttgct cacgcacgag gatttcggca tcgccaacgc cgccaaggaa gccttggcgt
     4081 tcgccattct ggcgcacgaa accttgctcg ggaaaccggg caatgtcccg agcgcgaccg
     4141 gcgcgcggcg ggcagtagtg ctgggcaaga ttgtgccggg atgataatga gtgggtacga
     4201 agtttcacct taaccatgca attgaccgaa gacctatcaa gccgcggaga cgcaaccgag
     4261 tcggcgcgcg gacattcttg tccgcagcga tgtggagcgg tatgcgagcc aaagacgagt
     4321 tcggagccgc gcggcgttcg gacattgctg cggacaagaa tgtccgcgcg ccgatgcagt
     4381 ctccaaccaa gctcgaaatg agaagcctat gggcaggttg aacatcaaac cggacggttc
     4441 ctgcaaatat gacctggtcg cgctgggaga gatcatgctg cggctggacc cgggcgaggg
     4501 gcgcgtgcgc acggcgcgcg agttcaaggt ctgggaaggc ggcggcgaat acaacgtcgc
     4561 ccgtgccatg aagaaatgct ggggcaagcg ctccagtgtc gtcaccgcgc tgccgaagaa
     4621 cgacctcggc tggctggtgg aggatttcat ctgccagggc ggcgtcgaca cctcgcacat
     4681 catctggcgc gagttcgacg gcatcggccg c
//
LOCUS       DATCGS010000392         7138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_280757, whole genome shotgun sequence.
ACCESSION   DATCGS010000392 DATCGS010000000
VERSION     DATCGS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280757"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..2295
                     /locus_tag="VIL39_08045"
     CDS             412..2295
                     /locus_tag="VIL39_08045"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY5481415.1"
                     /translation="MRNTKWVGLVAALALPLLTAHAQTPEAPVPGAPAQAQAAVPVNL
                     SPGAAEVARLAGSGVGDDVVLAYIQNSQAPFTLSADDVLYLNDIGLSPQVTSAMLSHD
                     NALRGQPQQYAPAAPAPAAPPPVAEVPVAQPAPPPAVAPPPAYVTTAPADVSYFYNDL
                     SPYGTWVSLEGYGWCWQPQTVVIARGWRPYCDGGHWVYSDAGWFWQSDYSWGWAPFHY
                     GRWYQHPRCGWVWTPDRVWGPAWVTWRTGGDVCGWAPLPPHASFDVRLGWRYNGVSVG
                     ASFDFGLGIGAFAFVGFGDFGNHDLGHRRLPPERVTTIYRQTTIVNNYVVVNNNIVHR
                     GIPLERVTAASRVPVPRATVRDLPAGSARTPSRTASVVYRPQLQAPARPVKMVAQQVD
                     TRNPVIQHAPIAPVNAQRSSTLSRTGSAPGAASRQPAVQAPRTSTWSSGAKPASSPQY
                     QPTSKTPQPAATSATPRTSTWSNGAKPASSSQYQPTSKTPQPAATSATPRASPWSSGA
                     KPLSSSQSQSTSRTYQPVTKVPPAATTPRAPDEWKQGTRAAPGYRSDAGLPSIYNART
                     ASESSSATVKTTVQPQNSHVYQPKSQQQAAEIRALPQSNQRPSASPSSTGKSMDSKSG
                     KKQ"
     gene            2455..3924
                     /locus_tag="VIL39_08050"
     CDS             2455..3924
                     /locus_tag="VIL39_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5481416.1"
                     /translation="MKRLVLFIEPAGAQLNIFTQFTLPRLGSFILAGLVNRRDGWSAR
                     VFIEGRQRFDLQTWMAQHGRPDVVGISNITATVQRGYALADECRALGIPVILGGPHVT
                     FLPDEALGHGDLVVRGEGEGAMNALLDLWNEGPVAATDPRHATVPNLSWKDGAGTIHH
                     NDKAPWITDLDALPTPDFSLAGGTADCVIGGKKTVMVQTSRGCPFDCSFCSVTGMFGK
                     KFRYRSVPAILKELKQYDTRKHFVFFCDDNFTANKRRARELLEAMIAEGFHFQWSTQV
                     RTDLARDPELVRLMKRAGCHTVFIGFESVNPKSLTEMKKGQSLDDIRHAISVVQEAGI
                     HIHGMFVFGFEEDDWNTVEATVSFARQTKLTSVQLLILTPLPGSELYQRLLAEKRITS
                     FDWDLYDTHHVVYRSAGFTPFELQCAQVYGHTRLYTVPESLKKLVTGRLVATGLSFYA
                     WKINRDWQKNNQEYLRKLADGAAPAGWIDASHRPAASLS"
     gene            3921..5333
                     /locus_tag="VIL39_08055"
     CDS             3921..5333
                     /locus_tag="VIL39_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEY5481417.1"
                     /translation="MKKPNHRALLTLSLAMLSAQLGGQAAPSPNQPAGVTNRLLVVAP
                     GVFHPALQEFVAHKQKLLPTELRSLEDILKNTNGADDPEKLKRFLYAQWRERGLGYVL
                     LVGDVDVLPVRFMVLDRVTPAAFDYAFYPSDLYYSDLAKPDGSFEDWNARKEGFHAGY
                     FGEVRGEKNKTDPINYDEIDYLPEVAVGRWPVSTPAEARLVAAKTVAYERMVLAHSSP
                     QPQRAAFIATGGWVDSRQLMDELAAKLAGAWQLEKRYYSDARRPSATPPPDHQQVGQL
                     LNEGAGLVVHTGHGQPDGWDHCFSVGDLDHITNAMQLPVIISAGCSTAYFSPLPPYEP
                     YVDVDGKEHAGSDHKEVFNAPPPPPSPYQRGRFNPTGLGEQLLKRSTNGSVAYIGCNT
                     GSQPCGLTLVEGFVNALAEAKEPRLGDCWTSAIRHYFSKEKLATLKPNDDWYPPSIFF
                     QGMKFMLFGDPSLRLPANRP"
     gene            5847..6083
                     /locus_tag="VIL39_08060"
     CDS             5847..6083
                     /locus_tag="VIL39_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481418.1"
                     /translation="MGMNSTVLEPNKLRPEIIQRVEAMDDESLLVLHRVLLIVEKQRL
                     WRELAAEAEQDRRLGRFERLPEIIREARAELRQG"
     gene            6149..6505
                     /locus_tag="VIL39_08065"
     CDS             6149..6505
                     /locus_tag="VIL39_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5481419.1"
                     /translation="MDAWVAGHLTWAVSTPILLEYEEVLTRLSGPARWRKLARLMDLA
                     ELTSGNLLRVTPSFQFRIVTADPDDNIFADCAITAQADYLLTEDAHFAPLADAGYKPR
                     PLSPPQFIAQFFASGK"
     gene            6872..7102
                     /locus_tag="VIL39_08070"
     CDS             6872..7102
                     /locus_tag="VIL39_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481420.1"
                     /translation="MEAIDEEALKPRRRGWYLGSEQFKEQMLERMEGKLGESHSGELH
                     RETAEQRANRIIVEELARRGWPEFWLRPPRQL"
BASE COUNT         1342 a         2262 c         2218 g         1316 t
ORIGIN      
        1 agacaaaaca cccgcggcgg ttgatttgag gaagcacctg cgtgagacgt tcgtaagcat
       61 attcattatc cttctattaa tgacgcgtgt tcatgtagat cattacgaca ttccccggtg
      121 cgattcttcc gctccgggaa gcccgtcgaa tcacccaagt gaaacactat cagcgcccaa
      181 cgccaaccca aaaatgaccc actcaagcat gcctgccgag catggttgcg cccctttccg
      241 ccgggctgga acccaaggct cacggtcggg ctgaaagccg gccgccagta tgacgcgcgg
      301 atttgatggc gctttcttcg tatctagctt aatatttggc agattcgtcg tctgaataaa
      361 ccttggttgg cgcggtctaa ccgattaata cagaacgcgg gttcgattat tatgagaaat
      421 actaaatggg tcgggttggt tgcagcgttg gctttgccat tactgactgc acatgctcaa
      481 acaccggagg cgccagttcc aggtgctccg gcgcaagccc aggcagcggt cccagtcaat
      541 ctttcgccgg gtgcggccga agtcgcccgg ttggccggct cgggtgttgg ggacgatgtg
      601 gtcctggctt acatccagaa ttcgcaggcc ccgttcacgc tttccgcgga cgatgtcctt
      661 tatctcaatg atatcggcct gtccccgcag gtgacctcgg cgatgctgag ccacgacaac
      721 gcgctgcggg gccaacctca gcaatatgcg ccggcagctc cggccccagc cgcgccgcct
      781 ccagtcgccg aggtccccgt cgcgcagcca gcgccgccgc cagccgtcgc tccgccgccg
      841 gcctatgtca ccaccgcgcc ggccgatgtg agttacttct acaatgatct ttcgccctac
      901 ggcacctggg tgtccctgga aggatacggc tggtgctggc aaccccagac ggtcgtgatc
      961 gcccgcggct ggcggcccta ctgcgatggc ggccactggg tttactcgga cgccggctgg
     1021 ttttggcaat ccgactattc gtggggctgg gcgccgtttc actatggccg ctggtaccaa
     1081 cacccgcgct gcggctgggt ctggacgccc gaccgggtct ggggtccggc ctgggtgacg
     1141 tggcggacgg gcggggacgt ctgcggctgg gcaccgctgc cgccgcacgc cagctttgat
     1201 gtgcggctgg gctggcgcta caatggcgtc agcgtggggg cgagcttcga ctttggcctg
     1261 ggcatcggcg cctttgcttt cgtcggcttt ggcgacttcg gcaatcacga cctcggccat
     1321 cgccgcttgc cgccggagcg agttacgacg atttaccgcc agaccaccat cgtcaataac
     1381 tatgttgtgg tgaacaacaa catcgtgcat cgcggcattc cgctcgagcg cgtgaccgca
     1441 gcctcgcgtg tcccggtccc cagggcgacc gtccgcgatt tgccagccgg ttctgccaga
     1501 acgcccagcc ggacggcgtc agtggtctat cggccgcagc tccaggcgcc cgcacgaccg
     1561 gtgaagatgg tggcccagca agtagatacg cgcaatccgg tcattcagca cgccccgatt
     1621 gcgccggtga atgcgcagcg ctcttcgacc ttgagccgca ccggatcggc ccccggcgcg
     1681 gcttcacgcc aaccggcagt gcaagcgccc agaacctcca cctggtcgag cggtgcgaag
     1741 cccgcatctt ctccgcaata ccagccaacc tcgaagaccc ctcagccggc ggcaacatcc
     1801 gcgacgccga gaacctccac ctggtcgaac ggggccaagc ccgcgtcttc ttcacaatac
     1861 cagccaacct cgaagacccc tcaaccggcg gcaacatccg caacgcctag agcctccccc
     1921 tggtcgagcg gtgcgaagcc cttatcttct tcgcaatccc agtcaacgtc gaggacctat
     1981 cagccggtca cgaaggtgcc gccagccgcg accacacccc gggctcccga cgaatggaag
     2041 cagggaaccc gcgctgctcc aggctaccgg tctgatgccg gcttgccgtc catatacaac
     2101 gcccgcactg ctagtgaatc gtcgtccgcg actgtgaaga cgacggttca gccacagaat
     2161 tcccatgttt accagcctaa gagccagcag caagcggctg agatccgtgc gctccctcaa
     2221 tccaatcagc gcccatcggc ttcaccttcg tccaccggaa agagtatgga ctccaagtcc
     2281 gggaaaaaac agtaatgacc agacgctaag ccccaactca gcgttcttgt ctggctcgga
     2341 agaaggcctg cggcgcgtcg gtcaccgcaa tgcaagcaga atggggtttc cctcaggggg
     2401 gattttgatt acaacccagg cgatgtttgc gcgaccactg aaagggcttt gtccatgaaa
     2461 cgtctggtgc tgttcattga gccggcgggt gcgcagctca atatcttcac ccagttcacc
     2521 ttgccaaggc tggggagctt catcctggcc gggttggtga accgccgcga cggctggagc
     2581 gcgcgggtgt ttatcgaggg ccggcagcgc ttcgatctgc agacctggat ggctcagcat
     2641 ggccggcccg acgtggtggg catctcgaac attaccgcca cagtgcaacg cggctacgcg
     2701 ctggcggatg aatgccgcgc gctcggtatc ccggtcatcc tgggcgggcc gcatgtcacc
     2761 ttcctgcccg acgaagcgct agggcatggg gacctcgttg tgcgcggaga aggcgaaggg
     2821 gcgatgaacg cgctgctcga cctctggaac gaaggccccg tggcggcaac cgaccccagg
     2881 cacgccacgg ttccaaacct ctcgtggaag gatggcgcgg gcaccattca ccataatgac
     2941 aaggctccct ggatcaccga cctggatgcg ctgccgacgc cggatttctc gctggctggg
     3001 ggaacggccg actgcgtgat cggcggcaag aaaaccgtga tggtgcagac ctcacgcggc
     3061 tgcccgttcg attgctcgtt ctgctcggtg acgggcatgt tcgggaagaa gttccgttac
     3121 cgctcggtcc ccgccatcct gaaagaactc aagcagtacg acacccggaa gcacttcgta
     3181 ttcttctgcg atgacaactt cacggccaac aagcgccgcg cccgggaact gctcgaggcg
     3241 atgattgccg aaggcttcca cttccaatgg tccacgcagg tgcgcaccga cctggcccgc
     3301 gatcccgagc tcgtgcggct gatgaaacgc gcggggtgtc atactgtgtt catcggcttc
     3361 gaatccgtga atcccaagag cctcacggaa atgaagaagg gccagagcct agacgacatc
     3421 cgccacgcca tcagcgtcgt ccaagaagcc gggatccaca tccatggcat gttcgtgttc
     3481 ggcttcgaag aagacgactg gaacaccgtc gaggccacgg tcagctttgc ccgccagacg
     3541 aagctcacga gcgtgcagtt gctcattctg accccgctgc ccgggtcgga gctttaccag
     3601 cgtctgttgg ccgaaaagcg gattacgtcc ttcgactggg atctctacga tacgcatcac
     3661 gtggtctatc ggtcggcggg tttcaccccg ttcgagctcc agtgtgccca ggtgtacggg
     3721 catacgcggc tctataccgt gcctgagagt cttaagaagc tggttaccgg caggttggtg
     3781 gcgacgggcc tgtcgttcta tgcctggaag atcaaccggg actggcagaa gaacaaccag
     3841 gagtatctcc gcaagctggc ggacggagcc gccccggcag gctggatcga cgccagccac
     3901 cggccggccg cctccctttc atgaagaagc cgaatcatcg tgcgcttctc acgctgtcgc
     3961 tggcgatgct ctccgcccaa ctcgggggcc aggcggcacc atcacccaac cagcccgctg
     4021 gcgtaacgaa ccggttgctg gtggtcgcgc ccggcgtctt ccatcccgcc ctccaggaat
     4081 tcgtcgcgca caagcagaag cttctgccga cggaattgcg gtcgttggag gacattctaa
     4141 aaaacacgaa cggtgcggac gaccccgaga agctcaagcg cttcctgtac gcccaatggc
     4201 gtgagcgcgg cctgggctac gtgttgctgg tgggtgatgt ggatgtgttg cccgtgcgtt
     4261 tcatggtgct ggaccgggtt actccagcgg ccttcgacta cgccttctac ccgagcgatc
     4321 tctactacag cgatctcgcc aaaccggatg ggtcattcga ggactggaat gcccgcaagg
     4381 aaggcttcca tgccggctac ttcggcgagg ttcggggcga gaagaacaag accgacccga
     4441 tcaattacga tgagatagat tacctgcctg aagtcgcggt gggacgctgg ccggtcagca
     4501 cgcctgcgga ggcccgcctg gtggcggcca agacggtggc ctatgaacgg atggtgctcg
     4561 cccattccag cccgcagccg caacgggcgg cctttatcgc gaccggcggc tgggtggaca
     4621 gccgccaact catggatgaa ctcgcggcga agctggccgg cgcgtggcag ttggaaaaac
     4681 gttactactc ggatgcgcgc cgaccgtccg ccacaccgcc cccggaccat cagcaggttg
     4741 gccaactgtt gaacgagggc gcgggcctcg tggtccacac gggacacggc cagccggatg
     4801 gctgggacca ctgcttttcg gtcggcgacc tggaccacat caccaacgcc atgcagttgc
     4861 ctgtgatcat ctcggccggt tgcagcacgg cttacttctc gccgctgccg ccctatgagc
     4921 cgtacgtgga tgtggatggg aaggagcacg cgggctcgga ccacaaagag gtgttcaatg
     4981 cgcctccccc gccgccctca ccctaccagc gaggccgttt caacccgacc ggtctgggcg
     5041 agcaactcct gaaacgcagc acgaatggca gcgtggccta catcggctgc aacaccggca
     5101 gccagccgtg cggcttgacc ctcgtcgagg gatttgtgaa cgcgttggcc gaggcgaagg
     5161 aaccacgcct tggggattgt tggaccagcg ccatccgcca ctacttcagc aaggagaagc
     5221 tggcgacgct aaagccgaat gatgactggt atccgcccag catcttcttc caggggatga
     5281 agttcatgct gtttggagac cccagcttgc gcctgccggc caaccggccc tgatgactgc
     5341 acttaaggtg cccgcaggcg gtagaagcgg cgcggggtgt tggtggcctg caggtcggtg
     5401 aattggaact ggccgggcga gacttctgtg acgccgccca gcccccagct cgaattcaaa
     5461 tctacggcta acccctgtgc ttccactacg tcccgcatgg cgcatggcca aacccaaagg
     5521 gattgtatca ctcagcccag cgctggaccg ccggggcaaa gggggtggtc ctacgctgcg
     5581 agaacgtcgc gagacagcca tcaaccctat tgaaagcggt tgcatcaggc ccttgccatg
     5641 tagcgggagt cgtccagcgg ctgaggtgga ggcttcggcg ggcgacggct gggcgctttg
     5701 gcgtcagcca cagccctctg caaggcggcg tggaagacat ggggtttgcc aatgaagcga
     5761 agcgggaact ggagtccgcc cagggtcgaa acggttaccg tccccgcaac aaatggctcg
     5821 ctttgcccgc ggcagggcgg catattatgg gcatgaattc gaccgtgttg gagccgaata
     5881 aactgcgtcc ggaaatcatc cagcgcgttg aggcgatgga cgacgagagt ctgctggtgc
     5941 tccatcgggt tttgttgatc gtcgagaagc agcgactctg gcgtgagctg gcagcggaag
     6001 ccgagcaaga ccgccggttg ggaaggtttg agcggctgcc cgagatcatc cgcgaggcgc
     6061 gcgccgaact gcgtcaagga tgatcgtctg cctcgatacc aacacggtcg tataggccct
     6121 ggcacagcac catcccttcc acccaatcct ggatgcctgg gtcgccggac atttgacttg
     6181 ggcggtatcc actcccattc tcctcgaata cgaagaggtc ctcacacgct tgagcggacc
     6241 ggctcgatgg cgcaagcttg cgcggctcat ggatctggcc gagctgacca gcggaaacct
     6301 cctccgcgtt acaccctcat tccagtttcg catcgtgacc gccgaccccg atgacaacat
     6361 cttcgccgac tgcgccatca ccgcgcaggc cgactacttg ctcaccgaag atgcccattt
     6421 cgcaccgctg gcagacgctg ggtacaagcc gcgccccctc tcgccgccgc agtttatcgc
     6481 ccaattcttt gccagcggca aatgaacggc ttggtggctg gtttctcggt tggttccgcg
     6541 gtgcctgaac cgggtggcgg gatgggcatg ccggcggggc agtcttccac atcaggccag
     6601 cctccgatgg ggtcgggccg taagcctggt tgacgggatg cccgaggact tccttggacc
     6661 agtctccggt ctgatcccag gtgaatcggc cggaccgagg gggcagaggc ggactgctgg
     6721 ttttcggtat ttgactggcg cgtggtggcc ggacatggtt gggttcatgc cgcgcgccat
     6781 gcgcgtggat cgctttctgg gcgagcacgg catggggcag gactccgcgg cggggcgtta
     6841 ggagttcgag cggcagatgg aaaggcgccg gatggaggcc atcgacgagg aagcgctcaa
     6901 gccgcggcgg cggggctggt acttgggcag cgagcagttc aaagagcaga tgctggagcg
     6961 gatggagggt aagcttggcg agagccactc tggggaactc caccgggaga ccgccgagca
     7021 aagggccaac cgcatcatcg tggaggaact ggcgcgccgg ggctggccgg agttctggct
     7081 ccgccccccc cggcaacttt gacaccccaa actttggacc ttggaccttg gactttgg
//
LOCUS       DATCGS010000393         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_529395, whole genome shotgun sequence.
ACCESSION   DATCGS010000393 DATCGS010000000
VERSION     DATCGS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529395"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VIL39_08075"
     CDS             complement(<1..1241)
                     /locus_tag="VIL39_08075"
                     /inference="COORDINATES: protein motif:HMM:NF028272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HEY5481421.1"
                     /translation="MNSALLYLQYHSVKNRTVMRIKRLKQPKYLVGAIVGGLYFYWYF
                     FRTLFGTPTRGQPFALLASPESQALLESIGALILLIILLVAWIIPHQRAALTFTEAEV
                     AFLFPAPISRRTLIHFKLLRSQTAILFTTLLLTLVTNRLGGHVWLRAAGFWLILSTVN
                     LHLLGSSFALTMLLDRGISPWQRRLGILALAVVLTGGVIVWGRQTMPAFDLSRFTDLQ
                     AVQDYAQQVLTAGPVPYLLYPLRLVVRPFLASNALAFLSALGPAAALLLLHYVWVVRS
                     NVAFEEASVQASQKLAEKLAAVRSGNWQAARKQPRSKRPPFRLRPSGPPAVALLWKNL
                     ISAGQAFTLRIWISLAVFALCASLGVTQASGGSGLRPALGMIAAMLLAWSLLIGPQLL
                     RQDFRQDLPLADLLKTYPLRG"
     gene            complement(1241..2035)
                     /locus_tag="VIL39_08080"
     CDS             complement(1241..2035)
                     /locus_tag="VIL39_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481422.1"
                     /translation="MIQVKALTKLYGEFVAVKELDFEVQPGEVMGLVGPNGAGKTTTL
                     RCLAGIIPPTRGSVHICGHDLAQEPIAAKQQLAFFTDEPRLFDYLTVEQHLAFTARIY
                     QVRNYEPLADQLLEELELAGKKNAVPGELSRGMKQKLAIACGLLHAPRVVCFDEPLTG
                     LDPIGIRRMKDSILKRAREGTAIIISSHLLHLVEEICSHILILKEGRKIIHGTLSEIT
                     QKFSAQAGDTSLEEVFFRATGDAEKGNAVETPASEMKPAAGPRSPP"
     gene            complement(2032..4116)
                     /locus_tag="VIL39_08085"
     CDS             complement(2032..4116)
                     /locus_tag="VIL39_08085"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEY5481423.1"
                     /translation="MVYIKPILYLLPLGWLWFRLIDHLRVGWSVYPQYAYGWAVPCLC
                     LYLLWRNAETRKCGNADMLESRYAKIPTPFSFSAFQLFSFFLLCFLYLPTRLIQEANP
                     EWRLVSWALALEVVAITLLLLRTIHYGPLTTTPPSSVAEPVLGSPAEGGLRSVARPRA
                     TCHVSRITYHVSRFTFPVCFFLVAVPWPTVIEGPVIQGLMRANTGVTVELLGVIGVPA
                     IQHGNVIEVATGSVGIDDACSGIRSLQATLMLALFFGELYALSARRRALCIFAGFVLA
                     FVFNVGRTLLLTWVASAKGVGAVASWHDPAGVTILVACFLSLWLIARGLQKAESRKQK
                     AESSGGKAEILKSETLKSSAQKAEGEEEKAESRNRNPVSAAFSMSGFQRFSIFLTAWL
                     LVVETGTEIWYRVQESHLPKAVAWRVEFPRDNPTYRESPLAEAAQRLLRPDEGISASW
                     TEAGQLQWQVVYLRWDPGRVAGYLAKSHTPQICMSGAGFNLRDSSPTRFFSLHGIQLP
                     FRSYVFERDGDSTHLYYCRWEDRPSKEMNVGGDAIRVALVRSVWTGRGNGGQRVLEVA
                     IRGIADQNEADAAFTRELEKLIRIEKPTDYGTTRPPEHETKDHRTTDVTQGQGPAGAC
                     PHAAQTLMTLCNRYEPPPDDHSGAETGQGKERNEDDDPNSELGLRISFGVRLSVFGVL
                     CGFAVPPSPFHL"
     gene            complement(4188..>4750)
                     /locus_tag="VIL39_08090"
     CDS             complement(4188..>4750)
                     /locus_tag="VIL39_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481424.1"
                     /translation="SQQPGFREPPIREGVRRLLKYDEGKQAEWRDVNGRPWQLYYLRW
                     FPARTRYRAVEAARGARLHEPVVCLTGAGMAPQKNFGSQVRQINGVTLLTNLERFSDK
                     GRPLHVLYCYWDSDPAALENRPASPPSTTSALRTAWRAVQVRDRARNEKCVIIIGVWG
                     METDEEAEAALRELLERGIRKEKLKS"
BASE COUNT          966 a         1389 c         1524 g          871 t
ORIGIN      
        1 cagccacgca gggggtaagt cttgaggagg tcggccaatg ggaggtcctg gcggaaatcc
       61 tggcggagca actgtggtcc gataaggagg gaccaagcca ggagcatggc cgcgatcatg
      121 ccgagcgccg ggcgcaagcc ggagccgccg gaggcttggg tcaccccgag cgaagcacag
      181 agggcaaaca cggcgaggct aatccagatg cgcaaggtga aggcctggcc ggcgctgatg
      241 aggttcttcc agagcagcgc gacggccggc ggaccgctgg ggcgcagcct gaacggcggg
      301 cgcttgctcc ggggctgctt ccgcgccgcc tgccaattgc cagagcgaac ggcggcgagc
      361 ttctcggcca gcttttggga ggcctggacc gaggcttcct caaacgcgac gttcgaccgc
      421 acgacccaga cgtagtgcag caacaggagg gccgccgccg gtccgagcgc ggaaagaaag
      481 gcaagcgcgt tcgaggccag gaaaggccga accaccagcc gcaacggata cagcaagtag
      541 ggcacggggc ccgcggtcag gacttgttgg gcgtaatcct ggacggcctg caggtccgtg
      601 aaccgggaga ggtcaaaggc aggcatggtc tgccggcccc agacgattac tccccccgtc
      661 agcacgactg ccaaggcaag aatgcccaaa cgccgctgcc agggactgat gccacggtcc
      721 agcagcatcg tcagggcaaa ggacgacccg agcaaatgca ggttcacggt ggagaggatc
      781 agccagaagc ccgcggcgcg aagccaaaca tggccgccca ggcggttggt caccaatgtc
      841 aggagcaggg tcgtgaacag aatggcggtt tgggagcgca gcagcttgaa atggatcagg
      901 gtgcggcggc tgatcggcgc gggaaagaga aaggcgacct cggcttcggt aaaggtcagc
      961 gccgcgcgct ggtggggaat aatccaggcc accaacagga tgatgagcag aatcagcgcg
     1021 ccgatggatt cgaggagggc ctgactctcc ggtgaggcaa gcagcgcaaa gggttgtccc
     1081 cgcgtgggcg tgccaaagag cgtgcggaag aaataccagt agaagtaaag cccgccaaca
     1141 atggcgccga ccaggtactt gggctgcttg agccgcttga tgcgcatcac cgtccggttc
     1201 ttgacggagt gatactggag gtagaggagg gcggagttca tcagggagga cttcgtgggc
     1261 cggcggcagg tttcatttct gaagcggggg tttccacggc gttgcctttc tccgcgtcac
     1321 cagtggcgcg gaagaaaacc tcttcgaggc tggtgtcgcc cgcctgggcg gagaacttct
     1381 gggtgatctc gctcagggtg ccgtggataa tcttccgccc ctctttcaag atgaggatgt
     1441 gcgagcagat ttcctcgacc aggtgcagga ggtgggagct gataatgatg gcagtccctt
     1501 cgcgggcgcg tttgaggatc gagtccttca tgcgccggat gccaatggga tcgaggccgg
     1561 tgagcggctc gtcaaagcag accacccggg gcgcgtggag caggccgcag gcgatggcca
     1621 gcttctgctt catgccgcgg gagagttcgc cggggacagc gttcttcttc ccggcgagtt
     1681 ccaattcttc gagcaactga tcggccagcg gctcgtagtt ccggacctga taaatgcggg
     1741 cggtgaaggc caggtgctgc tccaccgtca ggtaatcgaa gagccggggc tcatcggtga
     1801 agaacgccag ttgctgcttg gccgcgatcg gctcctgcgc gaggtcatgt ccgcaaatgt
     1861 gaacgctgcc gcgggtgggc ggaataatgc ccgccagaca gcgcagagtc gtggtcttgc
     1921 ccgcgccgtt gggcccgacc agtcccatga cttcgccggg ctggacttca aagtcgagtt
     1981 ccttcaccgc gacgaattcg ccgtataact tggtaagagc tttaacctgg atcataggtg
     2041 gaagggcgag ggaggaacgg cgaagccgca gaggactccg aaaaccgaga gccgaactcc
     2101 gaaggaaatc cgaaggccca actccgaatt cggatcgtca tcctcgttgc gctccttacc
     2161 ctggccggtt tcggcgccgg aatgatcatc ggggggcggc tcgtatctgt tacagagcgt
     2221 cataagtgtt tgcgccgcgt gagggcacgc gccagcgggg ccctggccct gggtgacgtc
     2281 agttgtcctg tggtccttgg tctcgtgttc cggtggtctc gtcgtcccgt aatcagtggg
     2341 cttttcgatt ctgatcagct tttccaactc gcgggtgaag gcggcatcgg cttcgttctg
     2401 gtcggcgatg cctcggatag cgacttcgag aacacgctgt ccgccattcc cccttcccgt
     2461 ccagactgaa cggactagtg caactcgaat ggcatctcca cccacgttca tttccttgct
     2521 cggacgatcc tcccaccggc aataatacag gtgcgtggag tcgccgtctc gttcaaacac
     2581 atagctgcgg aagggaagct gaattccatg cagagaaaag aatcgtgttg gggaagagtc
     2641 tctcaagttg aagcctgccc cagacatgca tatctgcggg gtgtgtgact ttgccagata
     2701 cccggctacg cggcccggat cccatcgcag ataaaccact tgccactgga gctgccccgc
     2761 ttctgtccaa ctggcgctaa tcccctcatc ggggcgcagc agccgctgcg ccgcttcggc
     2821 gagaggcgac tcacgataag tgggattgtc gcgcggaaac tccacccgcc aggcgacagc
     2881 tttgggcaag tgcgactcct ggacacggta ccagatctca gtgccggttt cgaccaccag
     2941 cagccaagcc gtgaggaaaa tgctgaaacg ctgaaagcct gacatactga aagcggccga
     3001 gaccgggttc cgatttctgc tttctgcttt ctcctcttcg ccttctgcct tctgcgctga
     3061 ggatttcagc gtttcagatt tcagtatttc agcttttcct ccgctgcttt ctgctttctg
     3121 ctttctgctt tctgcttttt gcagcccccg tgcgatgagc cacagggaaa ggaagcaggc
     3181 gacgaggatg gtgactccgg cggggtcgtg ccaggaagcg acggccccga caccctttgc
     3241 cgatgcgacc cacgtgagca acaacgttcg gcccacgttg aatacgaagg ccaacacgaa
     3301 gcccgcaaaa atgcaaagcg cacggcgcct ggcgcttagc gcatacagtt cgccgaagaa
     3361 cagggccagc atcagcgtag cctggaggga gcggatgccg ctgcacgcgt cgtcgatgcc
     3421 gacgctgccg gtggcgacct cgatgacgtt gccgtgctgg atggcgggaa ccccaatgac
     3481 gccgagcaat tcgaccgtca cgccggtgtt cgcccgcatc agcccctgaa tgactggccc
     3541 ctcgatgacg gtcggccagg ggacagccac gaggaagaag caaacgggga acgtgaagcg
     3601 tgatacgtga tacgtgatac gtgatacgtg acacgtagcc cttggcctgg ccacgcttcg
     3661 cagtccaccc tccgcagggc taccgaggac gggttctgct acggaggacg ggggggttgt
     3721 cgtcagtggt ccgtagtgga tggtcctcag caggagcagc gtaatcgcaa cgacctcgag
     3781 ggcgagggcc caactaacca gacgccattc ggggttggct tcctggatca ggcgcgtggg
     3841 caagtagaga aagcagagga ggaaaaagct gaaaagctga aaagctgaaa agctgaaagg
     3901 agtagggatt ttggcatatc ggctctccag catatcagca tttccgcatt tccgcgtttc
     3961 agcgttccgc cacagcagat agaggcacaa gcagggcacc gcccagccat aggcatattg
     4021 cgggtacacc gaccacccca cgcgcaggtg gtcaatgagc cggaaccaga gccagccaag
     4081 cgggagaaga tagagtattg gcttaatata aaccacgtga acgagctttt gcaggcgatc
     4141 gaatggtccg tggtttgcgg tccatgctct cgcccacttt cagcatgtca gcttttcagc
     4201 ttttcctttc ttatccctct ctctaaaagc tcccgaagtg ccgcctccgc ctcttcgtca
     4261 gtttccatgc cccaaacgcc aattattatg acgcacttct cgttgcgggc gcggtcacgg
     4321 acttggactg cgcgccatgc ggtgcgcaaa gcgcttgtgg tggaaggcgg gctggcgggg
     4381 cgattctcca gcgctgccgg gtcggagtcc cagtagcagt aaagcacatg cagcggccga
     4441 cctttatccg aaaacctttc gaggttggtc aacagggtga cgccattaat ctggcgcacc
     4501 tggctgccga aattcttctg aggcgccatc ccggcgcccg tcagacacac aaccggctca
     4561 tgcaaccggg cgccccgggc ggcctcgacg gcgcgatacc tggttcgggc cggaaaccag
     4621 cggaggtaat agagctgcca cggcctgccg ttcacgtccc gccactcggc ctgcttgcct
     4681 tcgtcgtact tcagcaggcg gcggaccccc tcccgaatcg gcggttcgcg aaatcccggt
     4741 tgctggcttg
//
LOCUS       DATCGS010000394         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_677605, whole genome shotgun sequence.
ACCESSION   DATCGS010000394 DATCGS010000000
VERSION     DATCGS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677605"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VIL39_08095"
     CDS             <1..1385
                     /locus_tag="VIL39_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481425.1"
                     /translation="LALMGSAKADTELQPAIKALAQALNAENLSKNDWTARIDQRLDE
                     VAKYYRELNELQEGINREEIAAEARQAIPTARTFERAQRNQDKVSAAESYSKLHEAAA
                     RVEKAQFVSGDYQGAHRLQSLTADSPLTARGKETADELRGLAMRTDNQPPSLAQAIPP
                     PMRQQTEALERQQSSAHDSAERLAWPRLALSVEASRLYRTKDRKAAAAYGLLGQDLGE
                     LLESPPQLTSANLRPLSERAAALAGQKGEEARQAEIRAAEERLQKLAQNAPRNPATLA
                     AQLDELSGAARQAAGNDKERAPLIERLGAVSGIAPPVADWTQSTQPEEVAASAAHESQ
                     TGIQAAPKAWASYNEASEILADAARQIRLNDAADQVAQLDPYPAPPEASPDDSIDAAR
                     PASGETVRMDGAAGQVVARPTPEGVDQAEWSRLREGLRQAIRNSGIEHFSEEQQSAIR
                     AYFERLSLEK"
     gene            1382..2467
                     /locus_tag="VIL39_08100"
     CDS             1382..2467
                     /locus_tag="VIL39_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY5481426.1"
                     /translation="MNYDYHRKSPVTTTLCLMFGLAGLALGRATLNAADLSDQEVLKR
                     LTTDQPRDKAVRRGLEYLRQQQKPDGSCSEKYPTALTGMAIMAHFAAGHPPADKQYGP
                     WLRKSINYVISKQNPDGYFGQSDGSRMYGHGIATLMLAEALGMPRDDEMDDSIRKALK
                     KAAQVTITAALIHKAPEHAGGWRYTPDATDSDVSLSAWQLMSLHAAQQVGLPVPEAII
                     TNAVNYTRRMIGPDGKVGYQNPNDEHPALRGVALLTLVIGHRERDPIIKKIADRIWAD
                     PIAWKGEHFYYRAYYDAVGISRALPEEWEKYLPKYEAVLLPHQREDGSFDTSVDGEAQ
                     GAGPTYTTSMAVMALAVQRYVLPAYQR"
BASE COUNT          665 a          967 c          872 g          504 t
ORIGIN      
        1 cgttggccct tatgggctcg gcgaaggccg atacggaact tcaaccggcg ataaaggcgt
       61 tggctcaggc acttaacgca gagaatctca gcaagaacga ctggactgcc cggatcgacc
      121 agcggctgga tgaggttgcc aaatattacc gcgaactgaa tgaactgcag gaaggtatca
      181 accgggaaga aatcgccgct gaagcccggc aggccattcc gacagcccgt accttcgaac
      241 gcgcccagcg gaaccaggat aaggtctccg ccgctgaatc ctactccaaa cttcacgaag
      301 cggcggcgcg agttgaaaaa gcacaatttg tttccggcga ctaccagggc gcgcaccgct
      361 tgcagtcgct caccgcagac tcgcccctga cggccagagg gaaagaaaca gcggatgaac
      421 tgcgcggctt ggcgatgcgc accgataacc agccaccgag tctcgcacag gccattccgc
      481 ctccgatgcg gcagcaaacg gaggcactgg agcgccagca atcaagtgct catgattccg
      541 ccgagcgact ggcctggccg cgcctcgcct tgtcggtcga ggcctcgcgg ctctatcgca
      601 ccaaagatcg caaggcggca gccgcttacg ggttgctggg gcaggatctg ggagagctcc
      661 tggaatctcc accgcaattg acgagtgcga atcttcgtcc gctgtccgag cgtgcggcgg
      721 ccctggccgg acaaaagggc gaagaggccc gccaggccga gatccgtgcc gccgaagagc
      781 gtctccaaaa actcgcccaa aacgctccac gcaaccccgc tacgctcgcc gcgcaactcg
      841 acgaattgtc tggcgccgcc aggcaagccg ctggcaacga caaggaacgc gctcccctca
      901 tcgaaagact tggcgcggtg tccgggatcg ctcccccagt ggccgattgg acccaatcca
      961 ctcagccgga ggaggtcgcc gccagcgcgg ctcacgagtc gcaaaccggc atccaggcag
     1021 cgccgaaagc ctgggcatcg tacaacgagg cttctgaaat cctcgccgac gccgcccggc
     1081 aaatccggct caacgacgcc gccgaccagg tggctcagct tgacccctac ccagcgcccc
     1141 ccgaggcctc acccgacgat tcgattgacg ccgcccgccc ggcctccggc gaaaccgtcc
     1201 ggatggatgg cgccgcgggt caggttgttg cccgacctac gcctgagggc gtggaccaag
     1261 ccgagtggtc ccggcttaga gagggcctgc gccaggcgat acgcaatagc ggcatcgaac
     1321 atttctctga agaacaacaa tcggctatcc gcgcctattt tgaaaggtta agtttggaaa
     1381 aatgaactac gactatcatc ggaaatctcc tgtaaccact accctgtgcc taatgttcgg
     1441 tctggccggc cttgctcttg gccgggcgac gctcaacgcc gccgatctct ctgaccagga
     1501 agtgctgaaa agactgacca ctgatcaacc gcgcgacaaa gccgttcgcc gagggctgga
     1561 gtatctccgc cagcaacaga agcccgacgg ctcatgtagc gagaaatatc caaccgccct
     1621 caccgggatg gcgattatgg ctcatttcgc cgccggccat ccgccagcgg ataagcaata
     1681 cgggccgtgg ctgcgcaaga gtatcaatta cgtgatctcc aagcagaatc cggacggcta
     1741 cttcggccaa tccgacggca gccgcatgta cggtcatggg attgccaccc tcatgctggc
     1801 cgaggcgctc ggcatgccac gcgatgacga gatggacgac agtatccgca aagcgctcaa
     1861 gaaagcggcg caggtgacga tcaccgcggc gctgatccac aaagctcccg aacacgccgg
     1921 cggctggcgc tacacgccgg atgcgaccga ttccgacgta agcctttcgg cctggcagct
     1981 tatgagcctg cacgccgctc agcaagtggg gctgcccgtg ccggaggcca tcatcaccaa
     2041 cgccgtcaac tacacccgcc gcatgattgg gccggacggc aaagtcggct accagaatcc
     2101 aaacgacgaa catccggccc tgcgcggcgt ggctttgctg actctggtca ttgggcaccg
     2161 ggagagagac ccgatcatca agaagatcgc tgaccgcatt tgggctgatc ccattgcctg
     2221 gaaaggcgag catttctatt accgcgctta ttatgacgca gtcggcatca gccgcgccct
     2281 gccggaggaa tgggaaaagt acttgccgaa atacgaggcc gtcttgctgc cgcaccagcg
     2341 cgaagatgga agcttcgata cgtccgtgga tggcgaagcc cagggcgccg gccccaccta
     2401 caccaccagc atggcggtca tggcgttggc cgtgcagcgc tacgtcctgc cggcgtatca
     2461 acgataacga gacgtaggaa gacgatgcgc aatccctgac tgagcttcaa agtctcagac
     2521 ctcagtaaac tggcccaacc tcaactccct cagtttggtt gaacttttgg cggccccgcc
     2581 atcaggttaa ggtggtcgcg ctccatcgcc ctgaggttgg gcggtggcgc cacgatgact
     2641 gacccggaag cgttgcaact gatcttgggg ccggacaagc gcaatccgtg cttttgcctt
     2701 tacgtcgatg cggatgagaa agagctccac gtctattacg ggctagaagt gctggaggtg
     2761 gtgcccaacg accccgtact gtccaaaaac tcgcgtttcc gcttggaact cacgaggatc
     2821 cgacggtata cacaagtgta tgcctcaccg tagcagaatt cctggccaaa ctgcagccca
     2881 cctccgatcc tgccggggtg aaccatcttc agttgttcct catcggtctg gccggctgga
     2941 tcaaccgcaa tcagcagaac gcgatcgaat acctccaaga ggaggtccag gtcctcaggg
     3001 agcaactg
//
LOCUS       DATCGS010000395         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_578363, whole genome shotgun sequence.
ACCESSION   DATCGS010000395 DATCGS010000000
VERSION     DATCGS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578363"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VIL39_08105"
     CDS             <1..258
                     /locus_tag="VIL39_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEY5481427.1"
                     /translation="CDDTELFTDLHSYNVGTQNPFDKEDKEFDTPTLRELWRTAPYLH
                     DGSAATIRDVLTTRNPKDEHGKTSQLTAQQIDDLAEYVLSL"
     gene            complement(253..1779)
                     /locus_tag="VIL39_08110"
     CDS             complement(253..1779)
                     /locus_tag="VIL39_08110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY5481428.1"
                     /translation="MPASTLLLQVVGLHKSYNVPVLTDFGFELRGGEVHALIGSNGAG
                     KSTFARILCGLTPPNSGEMQFDGHRYAPRSKREAEHAGIVMVLQELNVIGTLSVAENI
                     FLNRLPHRAGFVRFAALHDQAREALARVGLGDLDPATPADQLGVGQQQLVEVAGALAH
                     NCRLLILDEPTAALTAPETDRLFDNIRRLQADGVGIIYISHRMDEIRRIANRVTVVRD
                     GRRVATHEAAEVTPASMVREMVGHDLPERKAAAERAAGEPALRVDRLRAGDRVRDVSF
                     QVRRGEILGLAGLIGSGRTETLRAIFGADKKDGGEVFLGSATQPVVIREPADAVRAGI
                     GMVPEDRKQDGLLLAQSIRVNTTLATLPRHTRRGGWLDAKAETETTGSLCQRLDVRCA
                     SLEQAVSELSGGNQQKVVIGRWLARDCSVLLFDEPTRGIDVAAKDMVYQLLRDLAADG
                     KAVVFVSSELTELMAVCDRMLVMSAGRIAAEFLPGEWTQKKITHAAFSGYLDRQKAVS
                     "
     gene            complement(1936..2901)
                     /locus_tag="VIL39_08115"
     CDS             complement(1936..2901)
                     /locus_tag="VIL39_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY5481429.1"
                     /translation="MKHYFLPASLPSGLMVATCLCGVALLNGGCHKSDTKPEPGKPKI
                     ALVMKSLANEFFGTMAEGAKKHQAANAGAYELLVNGIKNETDLAEQVNLVEQMIARQA
                     NAIVIAPADSKALVTVLKRAKEAGILVVNIDNKLDADVLKQAGLVVPFVGPDNRAGAR
                     KVGETLAKQLKAGDKVAIIEGIPTAFNGQQRRLGFEDAMKAAGMNLVGVQSGNWEMDK
                     ANNIAAAMLSEHADLKAILCANDNMALGAVAAIQSAGKTGKVLVVGFDNIGAVRPMIA
                     DGRLVATADQHADQLAVFGIEAALKILKGEAPPADQTTAVDLITK"
     gene            complement(3037..>3223)
                     /locus_tag="VIL39_08120"
     CDS             complement(3037..>3223)
                     /locus_tag="VIL39_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481430.1"
                     /translation="WHSIEKSGEPSDAAWRSGGFDGTTRQGQGKTLDFPAVWRLPPQK
                     RILTRRTVAKPPVLLGP"
BASE COUNT          553 a         1042 c         1022 g          606 t
ORIGIN      
        1 tgcgacgata cggagctgtt cacggatctg cactcctaca acgtcggcac gcagaacccc
       61 ttcgacaagg aagacaagga gtttgacacg ccgacgctgc gcgagctttg gcgcacagcg
      121 ccctacctgc atgacggctc ggctgccacg attcgcgacg tgctgaccac ccgcaacccg
      181 aaggacgagc acggcaagac gtctcagctc accgcgcaac agattgatga cctggccgag
      241 tatgtcctgt ccctatgaaa cggccttctg ccggtcgagg tagccgctga aggcggcgtg
      301 cgtgattttt ttctgagtcc attcgccggg caggaattcc gcggcaatgc gtccggcgga
      361 catcacgagc atgcggtcgc acaccgccat taactccgtc agctcgctgg acacgaaaac
      421 cacggctttg ccgtctgccg ccaggtcgcg cagcaactgg taaaccatgt ccttggccgc
      481 gacgtcgatg ccgcgcgtcg gttcgtcgaa cagcagcacg ctgcaatcgc gagcaagcca
      541 gcggccaatg acgaccttct gctggttgcc gccgcttagc tcggacactg cttgctccag
      601 cgacgcgcag cgcacgtcca gccgctggca gagcgagccc gtcgtctccg tttccgcctt
      661 ggcgtccaac cagccgcccc ggcgggtatg gcgaggcaac gtagccagcg tggtgttgac
      721 ccggatggat tgtgctagca acaggccgtc ctgcttgcgg tcttcgggga ccatcccgat
      781 gcccgcccgg accgcgtccg cgggctcgcg gatgaccact ggctgcgtcg cgctgccgag
      841 gaaaacttcg cccccgtcct tcttgtcggc gccgaaaatc gcgcgcaacg tctcggtgcg
      901 gcctgacccg atgaggccgg cgaggccgag tatttcgccg cgccgcactt gaaagctcac
      961 gtcccggacg cggtcacccg cgcgcaggcg gtccacgcgc agggccggtt cgccggcggc
     1021 ccgctcggca gccgcttttc gctcgggcaa gtcgtggccc accatctccc ggaccattga
     1081 agcgggcgtt acctcggcgg cctcgtgggt ggccacacgc cgtccgtcgc gcacgacagt
     1141 gacgcgattc gcaatgcggc ggatttcatc catgcgatgg ctgatgtaaa tgatgcccac
     1201 cccgtcggct tgcagccggc ggatgttgtc gaacagccgg tcggtctcgg gggcggtcag
     1261 cgcggcggta ggttcgtcca ggatcagaag gcggcagtta tgcgcgagtg cgccagcgac
     1321 ttcgacgagt tgctgctgcc ccacgccgag ctggtcagcg ggcgtggccg ggtcgaggtc
     1381 gcccagcccc acgcgcgcga gcgcctcgcg ggcctggtca tgcagggcgg cgaagcggac
     1441 aaacccggca cggtgcggca ggcggttgag gaagatgttc tcggcgacgc tcagcgtgcc
     1501 aatgacattc agctcctgca ggaccatgac gatccccgcg tgttccgcct cgcgcttgga
     1561 gcgcggcgca tagcggtggc cgtcgaactg catctcgccg ctgttgggag gggttaagcc
     1621 gcagaggatg cgcgcgaacg tggacttgcc cgcgccgttg ctgccgatga gcgcatgaac
     1681 ctcgccgccg cgcagctcaa agccgaagtc cgtcagcacc gggacgttgt agctcttatg
     1741 caggccgacg acctggagca agagggtaga tgcgggcatg gaaccaagag cttaaccaca
     1801 gaggcacata gaggcacaca gatgggaaga tccctttgcc aacgcgcttg gcgacgcgga
     1861 gccatcagag gaacatgacg cggctaagtg agttgatctg tgtccatctg tgttcatctg
     1921 tggttcaccc gctggttact ttgtaatcag atccaccgcg gtcgtctggt ccgcgggtgg
     1981 cgcctctccc ttcaatatct tcagtgccgc ctcgatgccg aacaccgcaa gctggtcggc
     2041 gtgctgatcg gcggtcgcaa cgagtcgccc gtcggcaatc atgggccgaa cggcgccaat
     2101 gttgtcgaag cccaccacca gcaccttccc ggtcttgccg gcgctttgaa tggccgcaac
     2161 ggccccgagg gccatgttgt cattcgcgca gagaatggcc ttgaggtcgg catgctcgct
     2221 gagcatcgcc gcggcgatgt tgttggcctt gtccatctcc cagttgccgc tctgtacgcc
     2281 gacgaggttc atgcccgccg ccttcatggc gtcctcgaag ccgagccgcc gctgctggcc
     2341 gttgaaggca gtggggatgc cttcaatgat ggccaccttg tcgcccgcct taagctgttt
     2401 ggccagcgtc tcgccgacct tccgagcgcc ggcacggttg tccggcccga cgaagggcac
     2461 gaccaggccc gcctgcttga gcacatcggc atcgagcttg ttatcaatgt tcacgacgag
     2521 tatgccggct tccttggctc gcttcagcac ggtcaccagg gccttggaat cggcgggggc
     2581 gatgacgatc gcgttggcct gccgcgcgat catctgttcc accaggttta cctgttcggc
     2641 caggtcggtc tcgttcttga tgccgttgac gaggagttcg taagcgccgg cgttggccgc
     2701 ctggtgcttt ttggcgccct cggccatggt gccgaagaac tcattggcca gcgacttcat
     2761 cacgagcgcg atcttgggtt tgccgggctc gggcttggta tcggatttgt ggcatccgcc
     2821 gttcaacagc gccacgccac aaaggcaagt cgcgaccatc aggccgctgg gaagggacgc
     2881 gggtaagaaa tagtgtttca tgcgaataag tctattgcca tggtgcgcaa caattccgca
     2941 atgccccccg ccccgtcaat cacaaagcgg ggattcaagc tcattgggcc gcaatggcaa
     3001 tggccgcgag cagcctcaga ccccaatggc gcttagttaa ggccccagca aaaccggggg
     3061 cttggcaacg gttcttcggg ttaagatgcg tttttgcggc ggcaaccgcc aaaccgccgg
     3121 gaagtcgagt gtctttccct ggccctgccg tgtggtgccg tcaaacccgc cggaacgcca
     3181 ggctgcatcg ctgggttctc cgctcttttc gatggaatgc caa
//
LOCUS       DATCGS010000396         4912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_627680, whole genome shotgun sequence.
ACCESSION   DATCGS010000396 DATCGS010000000
VERSION     DATCGS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627680"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..4383)
                     /locus_tag="VIL39_08125"
     CDS             complement(223..4383)
                     /locus_tag="VIL39_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HEY5481431.1"
                     /translation="MLRAELLHELFEQQADACTDKIALACGDERLTYGELERRANQLA
                     RFLRSRGVRRGDYVGLWLPRSMDAHIALLAILKAGAAYVPLDPEYPAERVGFILSDCQ
                     ASALVTTSELGHIVRLSSIPPLPKGEGRGEGEQAARPSKPPDATNSPCRLTNVRLGAF
                     SGEVLALDERQQEIAAQPSDRLNRADTGAAPDDLCYVIYTSGTTGKPKGVEIAHRSAC
                     HLVRAEGKLFQVQPADRVYQGFSLAFDASVEEVWLAFFAGATLVVGTGEMVRSGPALS
                     RKLADAGVTVLSCVPTLLAMMDEDVPTVRLLILGGEACPPDLVKRWWQPGRRVFNTYG
                     PTEATVIATYAECHPDEPVTIGQPVPNYLACVLDEQLSPAPPGVAGELCLGGIGLARG
                     YLGRPELTREKFISFGVDGRPAQRFYRTGDLARWTPDGSKLEFLGRIDSQVKIRGFRV
                     ELGEIESALLKCPGVQAAAVALREDGPGVRQLVAYLVPQSASPFDEKAIRAQLQTHLP
                     AYMVPALLETLPQLPTLASGKVDRKSLPAPRTRTAEERPDLVPPRTNLEKQILVVWEK
                     LLAPTPVSVRDDFFRDLGGDSLLAAIMVSELRRSPALRRLSMLDVYQQPTVEKLAARF
                     AQPQTKEGEHPREPKHPQESGLAGPLALPVPFWRHFFCGAAQFVSLFCILSFFALQWL
                     APYLTYTVLIEEDYDFVESILGAFASLIVLYPLMLMIPIAVKWIMIGRYRPGSYPLWG
                     TFYFRWWFTTTIEAAVPVGYLTGTPLLNIYLRLMGAKIGSNVHLDSDTFAIYDLLNIG
                     EDSSINPDSNLLGYTIENGRLKIGRITIGKRCFVGARSAVREDTVMEDDSALEDLSLL
                     PRGTVIPHGETWLGSPARRIVGQASRLPPGRLAPEPTRAGETPATTAGGTPAPLSGSA
                     APPRPSTQRRFLFGVLHGIGLLIFPVLVVAALFPGIVVMNELNYLDPYYWYLLLAPLV
                     GLSFIVLLALEIALVKWVLLGRVKPGRHPLHSFYYLRKWFADQTLDLSLDTLGPLYAS
                     VYLTPWYKLLGAKLGHGAEVSTASFISPDLLSIGDESFIADSVSLGAPRVRDGFMTLG
                     RNHIGKRSFIGNSAMLPPGTVIGDSVLIGCLSSPPPNPADALRADTTWMGSPPIFLPQ
                     RQKSTTFPEETTFHPTTKLRVQRAAIEFVRVITPSTCFIILISLLFSSLLLLHDYLNL
                     LDTLLLFPFLYLGCAIAAAVTTILAKWALVWRYHPRETPLWSTLVWRNELLNALHEHL
                     AEPFLIGALTGTPFICWYFRLLGAKIGRRVYMETTDLTEFDLVRMGDEAALNADCTIQ
                     THLFEDRVMKMSTIDIGPRCKVGARSLVLYDTRLEEGAALGDLSLLMKGETLPAGTSW
                     QGIPALPE"
BASE COUNT          966 a         1462 c         1632 g          852 t
ORIGIN      
        1 tatcgaatac accgacacgc tgatgctggc gacgtggatt acgcttacga gcgcctcggg
       61 taacggtggc ctcgtcactt tcagtgatct ttcagccgca ggcgcccgcc gcttctaccg
      121 catccggatt aattaaagcc atctacgagt ccggtcggct cttttggctg gaacagccgg
      181 acctgccagc gtgccgcaag ccaggagccc gcgggatact ttctattccg ggagagcggg
      241 gatgccttgc cagcttgtcc cggcgggcag cgtctcgccc ttcatcagca gcgagaggtc
      301 gcccagcgcc gcgccttctt cgaggcgcgt atcgtacagc acgagcgagc gggcgccgac
      361 tttgcagcgg ggcccgatgt cgatggtgga catcttcatg acgcggtcct cgaagaggtg
      421 cgtctgaata gtgcaatcgg cattcagcgc cgcctcgtcc cccatgcgca ccaggtcgaa
      481 ctcggttagg tctgtggtct ccatatagac gcggcgtccg atcttcgcgc ccagcaggcg
      541 gaagtaccag cagataaagg gcgtgccggt cagggcgccg atcaggaacg gttcggccag
      601 gtgctcgtgc agggcgttga gcagctcgtt gcgccacacc agggtgctcc agagcggggt
      661 ctcgcgcggg tggtagcgcc agacaagcgc ccacttcgcc aagatggtgg tgaccgcggc
      721 cgcgatggca cagcccaggt ataggaacgg gaagagcaac aatgtgtcca ggaggttgag
      781 atagtcgtgc aggaggagca gcgatgagaa caggaggctg atcaggatga taaagcaggt
      841 ggaaggggtg atgacgcgca cgaactcgat ggcggcgcgc tgtacccgca gtttcgtagt
      901 ggggtggaac gtggtctcct ccgggaacgt ggtgctcttt tgtcgctgtg gcaggaagat
      961 gggcggcgag cccatccagg tcgtgtctgc gcgcagcgcg tccgccgggt tgggcggggg
     1021 agaagaaagg cagccgatga ggacgctgtc gccaatgacc gtgcccggcg gcagcatggc
     1081 gctgttcccg atgaaggagc gcttgccgat gtgattccgc cccagggtca tgaagccgtc
     1141 gcgcacccgc ggcgcaccga gggagacgct atcggcaatg aagctttcgt cgccgatgga
     1201 gagcaggtcg ggcgagatga acgaggcggt ggacacttcg gcgccgtggc cgagcttggc
     1261 gcccagcagt ttataccacg gggttagata gaccgaggca tagagcggcc ccagggtatc
     1321 gagactcagg tcgagagttt gatcggcaaa ccatttgcgc aagtagtaga agctgtgcaa
     1381 cgggtgccgc cctggtttca ctctccctag cagcacccac ttcacgagag cgatctccag
     1441 cgcaagcaac acgatgaagg acaacccgac cagtggcgcc agcaacaggt accagtaata
     1501 cggatcaagg tagttcagct cattcatcac tacgatgccc gggaacaggg cggcgaccac
     1561 cagcaccgga aagatcagca ggccaatccc atgcaacacc ccgaacagga accgtcgttg
     1621 cgtgctcggg cgaggagggg cggcggagcc cgatagtggg gcaggcgtcc cgcctgccgt
     1681 cgttgcgggc gtctcgcccg cgcgcgtagg ttcgggggcg agacgcccgg gtggcaggcg
     1741 agacgcctgc cccactatgc ggcgggcggg agagccgagc catgtctcgc cgtgcggaat
     1801 gacggtcccg cgcggtaata gcgaaaggtc ctcgagggcc gagtcatcct ccatcaccgt
     1861 atcctcacgc actgcggagc gggcgccgac aaagcagcgt ttgccaatgg taatgcgccc
     1921 gatcttcaac cggccgttct cgatcgtgta acccagcagg ttcgagtccg ggttaatgct
     1981 ggagtcctcg ccgatgttga gcaggtcgta gatggcgaag gtatcgctgt cgagatgcac
     2041 attggagccg atcttcgcac ccatcagtcg gagatagata ttcagcagcg gagtgccggt
     2101 caggtagccg acgggcacgg cggcttcgat ggtcgtcgta aaccaccagc ggaagtagaa
     2161 cgttccccaa agcggatacg agccggggcg gtaacggcca atcataatcc acttcaccgc
     2221 gatcgggatc atcagcatca acggatacag cacgatgagg ctggcgaacg cgccgaggat
     2281 ggattcgacg aagtcgtagt cctcctcgat cagtacggta taggtgagat agggggccag
     2341 ccattgcaat gcgaagaagc tcaggatgca aaacaggctg acgaactggg cggccccgca
     2401 aaagaagtgc cgccagaaag gcaccgggag ggcgagcggc cccgcgagcc ctgattcttg
     2461 gggatgttta ggctcgcggg gatgctcgcc ctccttggtt tggggctgcg cgaaacgcgc
     2521 cgccagcttc tctacggtcg gctgctggta cacgtcgagc attgagagac ggcggagtgc
     2581 cgggcttctt ctcaattccg acaccatgat cgccgccagc agcgaatcgc cgcccaggtc
     2641 gcggaagaaa tcgtcccgca ctgagaccgg cgtcggggcg agcagcttct cccacacgac
     2701 cagtatctgc ttctccaggt tggtgcgagg tgggacgagg tcaggccgct cttccgccgt
     2761 ccgtgtgcgg ggcgcgggca gactcttgcg atcaaccttg ccgctggcga gcgtaggcaa
     2821 ttgggggagc gtttccagca gcgccggaac catgtaggcc ggcaggtgcg tctggagttg
     2881 ggcgcggatg gccttctcgt caaagggaga ggccgactgc ggcacgaggt aggcgacaag
     2941 ctgtcgaacg ccgggaccgt cctcgcgcag cgccaccgcc gccgcctgga caccggggca
     3001 cttcaacagt gccgactcga tctcccccag ttcaacccgg aacccgcgta tctttacctg
     3061 gctgtcgatg cggcccagga attccagctt gctgccgtcg ggagtccagc gggcaagatc
     3121 gccggtgcgg tagaaacgct gagcaggccg gccgtccacg ccgaaggaaa tgaacttctc
     3181 ccgcgtcaac tcgggccgtc ccaagtagcc gcgcgcaagg ccgattccgc ccaggcacaa
     3241 ttccccggcc acgcccggcg gggcagggct gagctgttcg tcgagaacgc aggccaggta
     3301 gttcggcacc ggctggccga tggtgacggg ttcgtccgga tggcactccg cgtaggtggc
     3361 gatgacagtg gcctcggttg gcccgtaagt gttgaagacg cggcgcccgg gctgccacca
     3421 gcgcttcacc aggtcgggcg ggcaggcttc gccgcccaga atcagcaggc gcacggtggg
     3481 gacgtcctcg tccatcatgg cgagcagcgt ggggacgcac gagaggacgg tcacgcccgc
     3541 gtcggcaagt ttccgtgaaa gcgccggccc gctccgaacc atttccccgg tgcccaccac
     3601 gagcgtcgcc ccggcaaaga atgccagcca tacttcctcg accgaggcat cgaaggccag
     3661 ggagaagccc tgatagacgc gatcggcggg ttggacctgg aagagcttcc cttcggcgcg
     3721 aacgaggtgg caggcgctgc gatgtgctat ctccacgccc ttgggcttcc cagtggtgcc
     3781 cgacgtatag atgacatagc aaaggtcatc gggcgccgcg ccggtgtccg cgcggttcag
     3841 ccggtcggag ggttgggcag caatctcctg ctggcgttca tccagcgcga ggacttcgcc
     3901 gctgaacgca ccgaggcgga cgttagtaag gcggcaaggc gagtttgttg catcgggcgg
     3961 tttggatgga cgggcagcct gttccccctc accccggccc tctcccttgg ggagcggggg
     4021 tatcgaggac agtctcacaa tatgtcccag ctcgctggtg gttaccaatg cgctggcctg
     4081 gcagtcggat agaataaagc ccacccgctc ggccggatat tccgggtcca gcggcacgta
     4141 ggctgcgccg gctttgagaa tggcgagcaa cgcgatatgt gcgtccattg agcgcggcag
     4201 ccaaagcccg acataatcgc cgcgccgcac gccccggctc cggagaaacc gcgccaactg
     4261 gttggcccgg cgctccagct cgccataggt tagccgctcg tccccgcacg cgagggcaat
     4321 cttgtcggta caggcgtcag cctgttgctc gaacaactcg tgcagcaact cggcgcgcag
     4381 caagcccggg tccggtgtgc cgtgaatgaa ctctggatac ttcagacgac ctcacgatag
     4441 acgtccgggc gcagacagta aatgacgacc gtatcagagg ctattggctc cgcatccggt
     4501 agaagccgag tgacgcgccg cgcggtgaca tgtcgctcca gtccatgggt aaggcggtcc
     4561 gaaactgata actgataggc atttgctttt tgcgcgtccg cttgtacttg gcgctgcgcg
     4621 ggtgagccgc atgagcgaac tggtttttga agcgaggcag gagaaagagg gggcttctgc
     4681 gctgaggcta atccgattat ccctgaataa tacaatctca ttaactgacc cctgcttcct
     4741 gcttgggcca gcggcagggt tatcggggtg gaagcctgca cgaaactagc caatcccacg
     4801 tgttctccgg tggggacagt cacccttgcc ggcgggtcgt ctgattatcg gtaaacaaac
     4861 aaacattaaa aacaagtaat aggttatgaa aaacacaaat agtttagttc tg
//
LOCUS       DATCGS010000397         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_379099, whole genome shotgun sequence.
ACCESSION   DATCGS010000397 DATCGS010000000
VERSION     DATCGS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379099"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VIL39_08130"
     CDS             complement(<1..1039)
                     /locus_tag="VIL39_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5481432.1"
                     /translation="MSIRTRLTLWYAGIMFVSLVAMGILSYHTFAPELRPSGHSTGDG
                     TKPDDQDEGDLGEVFRIIFWCGAPSILLALGGGWWMMRRALAPVAALTQAAERVNEHN
                     LGERLPRTGDGDELDRLTEVFNAMTERLNQSFARIREFTLHASHELKTPLTVMHGELE
                     TALQDKALPGPQREQLESELDEVQRLAKIVDGLTLLTKADAGQITLARQPVRLDELVR
                     EACADAQSLARPHDIQVGVTACEELTVTGDRHRLRQLLLNLTDNAIKYNQRKGTVTLS
                     LRRVAGNAEVAIANTGPGIPAETLPRVFDPFFRADASHSQTVEGCGLGLSIARWIVTA
                     HGGTIQITSEPN"
     gene            complement(1036..1710)
                     /locus_tag="VIL39_08135"
     CDS             complement(1036..1710)
                     /locus_tag="VIL39_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5481433.1"
                     /translation="MRVLVVEDEKKTASFIRKALQAEGFAVDVCHNGDEAWAATCATT
                     FDAIVLDIMLPGRDGLSLLRQLRERQNATPILLLSARGEVNERVEGLNAGADDYLPKP
                     FVIAELIARVRALGRRGSETKSPVLRVGDLALDTVTREARRGGVAIELTAREYRLLEF
                     LMRSAGRICGRMAILEKVWDYDFDPGSNLVDVNVMRLREKIDADFEPKLLHTVRGIGY
                     VMKESP"
     gene            complement(1749..1943)
                     /locus_tag="VIL39_08140"
     CDS             complement(1749..1943)
                     /locus_tag="VIL39_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481434.1"
                     /translation="MNTNIAKFTLLMALVALGLTPAQKGFAGQPTKWEDVPEAVRATV
                     LANGGSNLMRVAMGSGELVR"
     gene            complement(2087..2506)
                     /locus_tag="VIL39_08145"
     CDS             complement(2087..2506)
                     /locus_tag="VIL39_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY5481435.1"
                     /translation="MKIKSIICSIVTLGLLAGGLTACMTEKGESKEGEKATSAKLEAQ
                     AKITKAEAQKIALDKVPGGTIKEGDIEKEKGKLLWSFDIATPGTKDITEVQVDAMTGD
                     VLDVAKETVADQEKEKKEDSKMKAKKEKDDDDKEETK"
BASE COUNT          490 a          905 c          875 g          620 t
ORIGIN      
        1 gattcggctc ggacgtgatt tgaatggttc ccccgtgggc ggtgacgatc caccgggcta
       61 tactcagacc cagaccgcac ccttcgacgg tttggctgtg gctggcatca gcgcggaaga
      121 acgggtcgaa gacgcgcggc agggtctccg ccgggatacc cgggccggta ttggcaatgg
      181 ccacttcggc gtttccagcc acgcgccgca agctgagggt cacggttcct ttgcgttggt
      241 tgtatttgat ggcgttgtcg gtgaggttca gcagcaactg gcgcaaacgg tggcggtcgc
      301 ccgtcacggt cagttcctca caggccgtca cgccgacctg aatgtcatgc gggcgcgcga
      361 gactttgggc atcggcgcag gcttcccgaa ccagttcatc caggcgcacc ggttgacggg
      421 cgagggtgat ctgcccggca tcagccttgg tgagcagcgt gaggccgtcc acaatcttag
      481 ccaggcgctg cacttcatcc agctcgctct ccaactgttc ccgctgcggg ccggggagcg
      541 ctttatcctg gagggctgtt tccagttcgc cgtgcatgac cgtcagcggc gtcttgagtt
      601 catgcgacgc gtggagggtg aactcacgga tgcgcgcgaa cgattggttc aggcgctccg
      661 tcatggcgtt gaagacttcg gtcaggcggt caagctcgtc accatcgccg gtgcggggca
      721 ggcgttcgcc caggttatgc tcattgacgc gctcggcggc ctgcgtgaga gccgcgaccg
      781 gcgcgagcgc ccggcgcatc atccaccagc cgccccccag cgccagcagg atggagggcg
      841 ccccgcacca gaagatgatc cggaagactt ccccaaggtc accttcgtcc tggtcgtctg
      901 gtttggtgcc atcgccggtg ctgtgcccgg aaggccgcag ctcaggggcg aatgtgtgat
      961 aagacagtat tcccatcgcc accagcgaaa cgaacatgat gccggcatac cacaaggtca
     1021 ggcgggtgcg gatgctcatg gcgattcctt catcacgtag ccaatgcccc ggacggtatg
     1081 gagcagcttg ggctcgaagt cggcgtcaat tttctcccgc aggcgcatga catttacgtc
     1141 caccaggttg ctgcccgggt cgaaatcata gtcccagacc ttttcgagaa tggccatgcg
     1201 cccacagatg cgtccggccg agcgcatgag aaactccagc aaccggtatt cgcgcgcggt
     1261 caactcaatg gcgacgccgc cgcgccgggc ctcgcgtgtg acggtgtcca acgccaggtc
     1321 ccccacgcgc aggacgggtg acttcgtctc gctgccgcgc cggcccaggg cgcggacgcg
     1381 cgcgatcaat tccgcgatca caaaaggctt gggcaggtaa tcgtccgcgc cggcattgag
     1441 gccctcgacg cgctcattga cttcgccgcg cgcggagagc agcaggatcg gcgtggcgtt
     1501 ttggcgttcg cgcaactgtc gcagcaggct caagccgtcg cggcccggca gcatgatgtc
     1561 gagcacgatg gcgtcaaacg tggttgcgca cgtggcggcc cacgcctcgt cgccgttatg
     1621 gcagacatcc accgcaaagc cctccgcctg gagcgccttg cgaataaacg aggcagtctt
     1681 cttttcgtct tcgacgacga gcacacgcat gaatgcagac tatacgggcc ggaatgcctg
     1741 cccaatgctt atctcaccag ttcgccgctc cccattgcca cgcgcattag attgctgccc
     1801 ccgttcgcca gaacggtggc gcgcacagct tcgggcacgt cctcccattt cgtgggttgg
     1861 ccggcgaagc ccttttgcgc cggcgttaaa cccagcgcca ccagagccat gagtagtgtg
     1921 aatttcgcta tgtttgtatt cattgcatca ttcccttctg tctcgggtgc cgccgctgga
     1981 gcgcggacat tcctgtccgc agcaacttcc ggacgcctcg cgatgtcggc tgttcactgg
     2041 ttcacttcca acgccgctgc ggacaggaat gtccgcgctt cggaatctac ttcgtctctt
     2101 ccttgtcgtc gtcgtccttt tccttcttgg ccttcatctt ggagtcttcc ttcttctcct
     2161 tctcctggtc ggcgaccgtt tctttggcaa catcaaggac gtcaccggtc atggcgtcca
     2221 cctgcacttc agtgatatcc ttggtgcccg gggtggcgat gtcaaacgac cagagcagct
     2281 tgcccttttc tttctcgatg tcgccttcct tgatggtgcc gcccggaacc ttgtccaacg
     2341 cgatcttttg cgcctcagct ttggtgatct tggcctgggc ttccagtttc gccgatgtgg
     2401 ccttttcgcc ttctttgctt tctccctttt ccgtcatgca agccgtgagg ccaccagcga
     2461 gcagaccgag tgtgactatc gaacagatga tggatttgat tttcatattt gcatttagtg
     2521 tttgtctcgc cgggaatact cccggcttcg cgtagtgcac catagcagcg tgacagggat
     2581 atgaacctcc agtgacagtt ttgtcattcc ccgagggaaa ggggggagga gccgtgatgc
     2641 gcgggcgcat ccatacactg gcatcgggta gcggcgggca tcctgcctgc cgtggagggc
     2701 ggcatcctgc cgtccgaact tcggtccgta gttctccacc ggatggcaat ccgaacaaca
     2761 attccgccgg gctgggaagc ccggctctac ggcaggcagg atgcccgccg ctacggcaca
     2821 gggcgtgaca ccaaaggttg accgggcagc ggtttacgcc tagaaagtgg gaaatgatgc
     2881 acagtctctt
//
LOCUS       DATCGS010000398         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_183361, whole genome shotgun sequence.
ACCESSION   DATCGS010000398 DATCGS010000000
VERSION     DATCGS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183361"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2961
                     /locus_tag="VIL39_08150"
     CDS             <1..2961
                     /locus_tag="VIL39_08150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5481436.1"
                     /translation="FLFLRAQRSLPTELSAYANLDRFAQVEQAAKEQQLVRQLEAWLF
                     PPKPAHFDSPRAFLRAICRPQPDEEQPARYRLAVELHLLRPRTGEKVRTLGEIVELTT
                     RAAHEQELFPPGDWEFIQWLAETHANRSDGEETMVLSDLDLLHWLARWGHTTRLELAA
                     GSEPAQALEFHGEVAELTPHLENGEKELAFTHRLTVPGGKAYPLSEVRFFNRHPALTL
                     VGRTFYLLRNPPPPQVIEHWASEPAVPMRKLSHRLLMHLRQTQSSHGVDWEQLCVAHA
                     AKPEFIFELLDETVRLRLLARSQRDQSVWLWSGHEWQLHEAKKPRPAGNKPEILDDPR
                     LEPATLWLRQLDWFTPEPGLWVGDANEGFLSLLARAWADRPQEADYLGNPGFSRLFLA
                     PRQLRPRLVVKGSGIDWLAVSAEWEQEGMRLTAADLQRLQTATGRFVKLPDSGWVELD
                     SNAVQSAHEAMAELGIDGLAPVAQRVGLDQAAHLDEEGLKRFGDSAQANALRERIKNF
                     EGLPAIQLPATVQADMRPYQKDGFDFLCHLTNIRLGGILADDMGLGKTLQTLAWLAWL
                     KERHGKDPKPSLVICPASVLHNWQREANRFTPHLKVLVLESGAERHNLRKQIPQHDLI
                     VTNYALLRRDLEELQKFAFRAAILDEAQFIKNPGAQVTQSVKQLKSEHRLALTGTPLE
                     NRLLDLWSIVDFIQPGYLGNQEHFTETYEPRGEDAESAQRIARRRLSAKLRPLLLRRL
                     KKHVAKDLPDRIEERRDCQLGDEQRKLYLAELRRSREQVMKTVAENGLNKSKIHVLAA
                     LTRLRQVCCHPRLVGSDTASGKTETLFELLEPLIAEGQKVLVFSQFVRMLQLLEEECR
                     QRQIPTHILTGQTKDRQQVVSTFQSDPNPAVFLLSLRAAGTGLNLTTASYVVLYDPWW
                     NPAVEAQAIDRSHRIGQTQTVNAYRLISPGTVEEKIWELQQSKAQTIADVLGEEGFAR
                     SLSKVDLEYLFSED"
     gene            3290..3817
                     /locus_tag="VIL39_08155"
     CDS             3290..3817
                     /locus_tag="VIL39_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481437.1"
                     /translation="MLGLAAGGKSLQKWSAARTAAAVHQENSSEIGPRWQRNCGRLRE
                     MIRVSASLLFVLLLATGCSTPQTARFSEQERADLIVRYYSDDTSYMLKPSMKEGKFPS
                     ILDRDAILKVAKQQPGRQLAVVILIHYNVESQAEAVKCNWKNLLTQVGYQRVVFLRSA
                     NGMRVNGLPVLASDG"
BASE COUNT          737 a         1133 c         1288 g          681 t
ORIGIN      
        1 ttcttgtttc tgcgcgcgca gcgctcgctg ccgacggagc tgagcgctta cgccaatctc
       61 gaccgctttg cccaggtgga acaggcggcg aaggaacagc agcttgtccg gcaactggag
      121 gcgtggctct tcccgcccaa accagcgcac ttcgattcgc cgcgcgcttt cttgcgggcg
      181 atttgccggc cacagccaga cgaggagcag ccggcgcgct accgcctggc cgtggagctg
      241 cacctgctgc gcccgcgcac gggcgagaag gtgaggacgc tgggggagat cgtcgagctg
      301 acgacgcggg cggcgcacga acaggaactc tttccgcccg gcgattggga attcattcag
      361 tggctggcgg agacgcatgc caaccgcagc gacggggagg aaacgatggt gctgtcggac
      421 ctggatctgc tgcattggct ggcgcgctgg ggtcacacga cgcggctcga actggccgcg
      481 ggcagcgagc cggcgcaggc gctggagttt cacggcgagg tggccgaatt gacgccgcac
      541 ctggaaaacg gcgagaagga gctggcgttc acgcaccggc tgacggtgcc gggcggcaag
      601 gcttacccgt tgagcgaagt gcggttcttc aaccgccatc ccgcgctgac gctggtgggg
      661 cggacgtttt atctgctgcg caacccgccg ccgccgcaag tgatcgagca ctgggccagc
      721 gagccggcgg tgccgatgcg caagctgagc catcgcctgc tgatgcatct gcgccagacg
      781 cagtcgagtc acggcgtgga ctgggagcag ctttgtgtgg cgcacgcggc gaaaccggag
      841 ttcatcttcg agctgctcga tgagaccgtg cggctccggc tgctggccag gagtcagcgc
      901 gaccaaagcg tctggctctg gagcggccac gagtggcagt tgcacgaggc aaaaaagcca
      961 cggccagccg gcaacaaacc tgaaattctg gatgacccgc gcctggagcc cgcgacgctt
     1021 tggctgcggc agcttgattg gttcacgccc gagcccgggc tgtgggtcgg cgatgcgaac
     1081 gagggcttcc tgagcctgct cgcgcgcgcc tgggctgacc ggccgcagga ggctgattat
     1141 ctcggcaatc ccggcttctc ccggcttttc ctggcgccgc gccaactgcg tccgcggctc
     1201 gtggtcaagg gcagcggcat tgattggctg gccgtttccg ccgagtggga gcaggaaggg
     1261 atgaggctta cggccgccga tttgcagcgc ttgcagacgg ccactgggcg gttcgtcaaa
     1321 ctgccggatt cgggctgggt ggaactggac tcgaatgcgg tgcagtcggc gcacgaagcg
     1381 atggctgagc tggggattga tgggctggcg ccggtggcgc agcgcgtcgg gctggatcag
     1441 gctgcccatt tggatgagga agggctgaag cggttcggcg attccgccca ggccaacgcg
     1501 ctgcgcgagc gaatcaagaa ttttgagggg ctccccgcca tccaactgcc cgcaacggtg
     1561 caggcggaca tgcggcccta tcagaaggac gggttcgact tcctgtgcca cctgaccaac
     1621 atccggctcg gcggcattct cgcggacgac atgggcctgg gcaagacctt gcagacgctt
     1681 gcgtggctgg cgtggctcaa agagcggcat gggaaagacc cgaaaccttc gctggtgatc
     1741 tgccccgcct cggtgctgca caactggcag cgggaggcca atcgtttcac gccgcatttg
     1801 aaggtgctcg tgttggagag cggcgcagag cggcacaact tgcggaagca gattccgcaa
     1861 catgacctga tcgtgacgaa ttacgccctg ctgcggcggg accttgagga gttgcagaag
     1921 ttcgctttcc gcgccgccat tctggacgag gcgcagttta tcaagaaccc cggcgcgcag
     1981 gtcacgcagt cggtgaagca gttgaagtcc gagcaccgcc tggcgctgac cggcacgccg
     2041 ctggagaacc ggttgctcga tttgtggagc atcgttgatt tcatccagcc gggctacctg
     2101 ggcaaccagg aacatttcac ggagacctac gagccgcgcg gcgaggatgc cgaatcagcg
     2161 cagcgcatcg cccggcggcg gctgtcggcc aagctgcgcc cgctgctgct gcggcggctc
     2221 aagaagcacg tggccaagga tttgcccgac cgcatcgaag agcgccgcga ctgccagctt
     2281 ggcgacgagc agcggaagct ttacctggcc gaactgcgcc gcagccgtga gcaggtgatg
     2341 aagacggtgg ccgagaacgg cctcaacaag agcaagattc acgtgctggc cgccctcacg
     2401 cggctgcgcc aggtttgctg ccatccgagg ctcgtaggca gcgacaccgc ctctggcaag
     2461 acggagactt tgttcgagct gctggagccg ctgatcgccg agggccaaaa ggtgctggtg
     2521 ttcagccagt tcgtgcggat gctgcaatta ctcgaagagg aatgccgcca gcgccagatc
     2581 cccacccata tcctcaccgg ccagaccaag gaccgtcagc aggtcgtcag cacgttccag
     2641 agcgacccca acccggcggt cttcctgctc agcctccggg cggccggcac cggcttgaac
     2701 ctgacgacgg cgagttacgt ggtgctatac gatccgtggt ggaacccggc ggtggaagcg
     2761 caggccattg accgatcgca ccgcatcggc cagacccaaa ccgtcaacgc ctatcgcctc
     2821 atctcgcccg gcaccgtgga agagaagatc tgggagctgc aacagagcaa ggcccagacg
     2881 attgccgacg tgcttggcga ggaaggcttc gcgcgcagcc tgtcgaaagt cgatttggag
     2941 tatttgttct cggaggattg aaggccgagc gtatcagggc cagttcctct ctaaatgaat
     3001 tatggacttg gacctggcag aactcatcag gcgccagcgc gcctaccgct ttcttctggg
     3061 cggaggagct tgctccgtgc gctggatggg aagaagcgca gcttcgttat ggatgaaaag
     3121 ggggccacat ccggcctgcc tcaagactga aagacgctgt ccggttcttg gacagccgca
     3181 gatccacaag cctgggctgg ttaagtttgg aggcgcaaaa tcgggctgaa tatgcactcg
     3241 cgcaaaaagc agcggacgcg aggagtttgt ctgtaaacgt catagcgtta tgctgggact
     3301 ggcggctggt ggcaaatcac tccagaaatg gtcggcggca cggacagccg ctgccgttca
     3361 ccaggagaat tccagcgaga tcggaccccg ctggcaaagg aattgcggca ggttgcgcga
     3421 aatgataaga gtctctgcgt cgcttttgtt tgtgctgctt ctggccactg gctgttcgac
     3481 cccgcagacc gctcggttta gcgagcagga gagagccgat ctcattgttc gctactacag
     3541 cgacgatacc agctatatgt taaagccctc aatgaaagag gggaagttcc cgagcattct
     3601 cgaccgcgat gccatcctta aggtggctaa gcagcaaccg ggtcgtcagt tggccgtggt
     3661 catcttgatt cactataatg tcgagagtca ggctgaagca gtgaagtgta attggaagaa
     3721 tctgctaacg caggtcggtt accagcgcgt cgtcttcctg cgttccgcaa acggaatgcg
     3781 ggttaacggc ctgccggtgc tggcgagcga cggttaaggc tggaccacct cgaagggaa
//
LOCUS       DATCGS010000399         2900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_529705, whole genome shotgun sequence.
ACCESSION   DATCGS010000399 DATCGS010000000
VERSION     DATCGS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529705"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1567
                     /locus_tag="VIL39_08160"
     CDS             131..1567
                     /locus_tag="VIL39_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481438.1"
                     /translation="MKAADLDILLQEGEGVMLEYKEALSPSFTRELVAFANTAGGRIL
                     PGVRDDSTVKGIADTNELRARIQDIARNCDPPIKILLQCIGEVTVVTVRESDSKPVQC
                     SDGFFWRQGAVTQKLRREEIRDLFQQAGAIRFDLSICPRFRYPDDFDPEKFGDWLRKS
                     TISRGGSVEDILVNVEAAERSGGKLLFRNAGALFFAREPRRFFNQAYITCLLFKGTSK
                     VNVLDRKDFAGGVVADVEDSLRFIERNTRTAYRIEKLQREEIPEYPVAALREAITNAV
                     MHRDWLIEGANVFVEIYEDRIEVSSPGGLPRGMRLEDLGSKSVRRNPLIADLLQRIAF
                     IEKAGTGIRRMRDGARDQGYPEPEFAAGGFFTAVFRPIPAVNEQVTPVVTQQVPSKYP
                     TSSPQVTPQVVALLRAVMQEASAAEIQSAMQLKDRIHFLKKHILPALEKGLIERTIPE
                     RPRSSRQRYRITPAGLAVFEHSEKEPQP"
     gene            1564..2900
                     /locus_tag="VIL39_08165"
                     /pseudo
     CDS             1564..2900
                     /locus_tag="VIL39_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869267.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
BASE COUNT          586 a          918 c          892 g          504 t
ORIGIN      
        1 cggacctccg ggttgcctcc gggttgctcg ccggattgca gaaatggagc gccaggatgg
       61 cggaagcctt gaacctgatc gtctgccacc aggggcgttt gatgctgaat actccgaggg
      121 aggccctgac atgaaagcgg ccgatctcga cattctgctc caggagggtg aaggcgtcat
      181 gctggagtac aaggaggccc tttccccttc cttcacccgt gaactggtag cctttgccaa
      241 caccgctggc ggcaggattc tgccgggagt tcgggacgac agtacggtaa agggaattgc
      301 cgataccaac gagttgcgcg cccgtatcca ggatattgcc cgaaactgcg atccgccgat
      361 aaagatcctg ctgcagtgca ttggcgaggt gactgtggtt acggtccgcg agagcgattc
      421 gaagccggtg cagtgcagcg acggcttctt ctggcggcaa ggcgcagtga cgcaaaagct
      481 gcgccgtgag gaaatccgtg atctcttcca gcaggccggg gctatccgct tcgatctgtc
      541 catctgcccg cgcttccgct atcccgatga tttcgacccg gaaaaattcg gcgactggct
      601 gcggaaaagc acgatctccc ggggcggctc ggtggaggac attctggtga acgtcgaggc
      661 ggcggaacga tcgggcggca agctactatt ccgcaacgcc ggggcgctat tcttcgcccg
      721 tgagccgcgg cgtttcttca atcaagccta tatcacctgc ctcctgttca agggaacgag
      781 caaggtgaat gtattggacc gcaaggattt tgccggggga gtcgtcgccg acgtcgagga
      841 cagtctgcgt ttcatcgagc gcaacacccg cacggcctac cgaatcgaga agctccagcg
      901 cgaggagatt ccggaatatc ccgtggccgc cctgcgcgag gccatcacca atgccgtcat
      961 gcaccgcgac tggctcatcg agggggccaa tgtctttgtc gagatctacg aggatcgcat
     1021 cgaggtctcc agtcccggcg gtctcccgcg ggggatgcgt ctcgaggatc tcggaagcaa
     1081 gagcgtgcgc cgcaacccac tgatcgcgga tctgctgcag cgcatcgcat ttatcgagaa
     1141 ggctggcacg ggcatccgac ggatgcgcga cggggctcgg gatcaaggat atcccgaacc
     1201 cgaattcgct gccggtggtt tctttaccgc tgtgttccgg ccgattcccg cggtgaacga
     1261 gcaagtcacc ccagtagtca cccagcaagt acccagcaag taccccacaa gtagcccaca
     1321 agtcacccca caagtcgtgg cactcctgcg cgccgtcatg caagaagcca gcgcggcaga
     1381 gattcaatcg gctatgcagt tgaaggatcg catccatttc ctgaagaaac acatcctccc
     1441 cgcccttgag aagggcttaa ttgaacggac catccctgaa aggccccgca gttcgagaca
     1501 gcggtaccgc atcacacccg ccgggcttgc ggtttttgag cactccgaaa aggagccgca
     1561 accatgacgc ccaccggcta caccgaagac tccctggtcg agcgaccggc catcgccctg
     1621 ctgagggatt tgggctggga gacgttcaac gccttttgcg agttcgatca cggcgccagc
     1681 acgctgggcc gcgagacccg ggccgaggtg atcctcaccg cccgcctgcg cccggccttg
     1741 cggcggctca acccgtacgc gccgcccgag gtcatcggtc aggccatcga ggagctgacc
     1801 cgcgaccgcg cacgcatgag cgcgaccgcc gccaatcgcg agatctatca cctgctcaag
     1861 aacggcgtgc gcgttcctgt gcccgatcct gaaggggacg gcgagacggt ggaagtggtg
     1921 cgagtggtgg attgggacga cccggccaat aacgactttc tggtctgctc ccagttctgg
     1981 atcacgggcg agatgcacac ccgccgcgcc gacctggtgg gctttgtcaa cggtctgccg
     2041 ctgcttttca tcgaactcaa ggccgcccac cgccgcctgg agaccgccta caccgacaac
     2101 ctgcgcgact acaaggacac cataccccat gttttctggc cgaacgccct gattatcctc
     2161 tccaacggca gccagagccg ggtgggcagc gtcaccgccg gctgggagca cttcgccgag
     2221 tggaagaagg tgggcagcga ggacgaggtg ggccgggtat cgctggatac catgcttcgc
     2281 ggcgtctgcg acccggcgcg gctgctggac ctggtggaga acttcaccct gtttcaggag
     2341 gtccccggcg ggctgatcaa gctgacggcc atgaaccatc agtatctggg cgtcaacaac
     2401 gcgttggagg ccctggccga catccggcag cgcgagggca agctgggcgt cttctggcac
     2461 acccagggca gcggtaagag cgtggctatg atgttcttcg cccagaaggt gctgcgcaag
     2521 gtgccgggca actggacctt tgtggtcgtc accgaccggc aggagctgga cgggcaaatc
     2581 tacaagcact tcgcctcggc aggggtggtg accgaaggcc gcgcccaggc ggaaagcagc
     2641 aagcacctgc ggcagttgct caccgaagat caccgctacg tcttcaccct gatccacaag
     2701 ttccgcaccg agaagggcga ggcgcacccg gtgctctccg aacgacgaga catcatcgtc
     2761 atcaccgacg aggcgcaccg tagccagtac gacaccatgg cgctgaacat gcgcacggcc
     2821 ctgcccaatg ccgctctatt acgagaaccg catccccgaa ctgcaactgg tcaacgacaa
     2881 cctcaacgac gacatggaac
//
LOCUS       DATCGS010000400         4798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_429187, whole genome shotgun sequence.
ACCESSION   DATCGS010000400 DATCGS010000000
VERSION     DATCGS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429187"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..819
                     /locus_tag="VIL39_08170"
     CDS             133..819
                     /locus_tag="VIL39_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5481439.1"
                     /translation="MIELIQFSWSPFCIVQRRILEFAGVRFKVTNIPGNDRSLVWRLT
                     RQRYYGVPIIRDGKAVLFEVSEDSQVIAKYLDTKLQLGLFPWDLEGVQSIVWRYIENE
                     IEGVGFKLNDIYWRKVVPPPDQLAFLRHMERKFGRGCLDQWRAQQKELLTQLEQRLVP
                     FEEMLLHQPFLLGQRPRFVDFDLYGILGNFLYSGQYRLPAAHTGLRKWHQRMTRMQGQ
                     STACDKKGHS"
     gene            845..1507
                     /locus_tag="VIL39_08175"
     CDS             845..1507
                     /locus_tag="VIL39_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481440.1"
                     /translation="MKTLLPWLCVVGLVIGLGWVYSAGQKKDAELAALREESQQLQKL
                     RAEMEETKGTHATAESDELVKLRKGNEDLLRLRNEVGQLRKENQQLSGQVQTAQAQVQ
                     GAQAQAQALRANAAQAPAPGQLDPAAQAAFAARYGLAPQTPEQQQASVCINNLRLIDG
                     AKQQWALEKQKPAGALLTAADLAPYLKTNTLPTCPAGGVYTLNPVGFSPLCNIPGHAL
                     GK"
     gene            complement(1645..3228)
                     /locus_tag="VIL39_08180"
     CDS             complement(1645..3228)
                     /locus_tag="VIL39_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY5481441.1"
                     /translation="MNSYFSLGDWAVILLYVLGIIGLGVWFGKDQHNTRDYFLGSKTV
                     PWWGIGLSIVAAETSALTIIGVPAMAYGGNIVFLQMIIGYVIARIILAIVLVPHYFKG
                     EIYSPYQLFANTFGPSARQTAGAFFLISETLAAGVRVYVASIPVKLILGDKFLGFGTG
                     DPILGSILLFVVLSLLYTYIGGVKAVIWTDAVQFGLFLLGGVFALCYIPTLVDGGVGA
                     VFSQALAAGKLHWLNVTPPPGVSLTKFLLGAPFNIWMGVIGGTVVVLSSHGAEQLIVQ
                     RVLACKSVADGRKALALSAVVIFPLFFIFLLVGAMLWVFYQGHPFQIPLPETRPGSGI
                     TANDFIFPIFMMTEVPHLLKGFLIVAILSAAMSSVSSALTSLASVSTMDFVKHFLPGR
                     SEGFFLRFSKVSTVVWAAVLILTAYMSRQVPFVLNAAFALRGLTSGALVGGLALAVFW
                     KQGRSIPVITGMIVSLVTMTAIQLLPKLDCTKAIWMRIVGTEIFWPWYTLIGAIVTLL
                     TAWLVRTLLPEPKPVTPVS"
     gene            3332..4522
                     /locus_tag="VIL39_08185"
     CDS             3332..4522
                     /locus_tag="VIL39_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5481442.1"
                     /translation="MKLSLIVAATLLAANSPAADWPQWRGPNRNGISSESVSASWAAD
                     GPKVLWRASVGTGFSSISISLGRAYTMGNTNNEDTVWCFDAGTGRKIWQHTYAAQLGP
                     QWYEGGPSSTPTICSNRVFTISKWGDVFCLDAAKGTVLWQRDLRQDGVKPNRWGFAGS
                     PLIWGNLVILNAGTAGIALDRSTGRIAWSNGTNAGGYASPTRFVSGGKESVLIFAAKH
                     LVALDPQSGRELWRQLWETDWDTNNTDPLVHGGSIFISSFNRGCGLFSVASGIPEITY
                     TNKVLYNHLSSGILAGDYLYAFNGEAKKDTDFRCIHVPTGQLKWARKDPPFGALILAG
                     DKLIVLSEKGELLVGQASPEEFKPLARAKVLSGVCWTPPALADGRLYVRNARGELHCL
                     ELPR"
BASE COUNT         1012 a         1435 c         1522 g          829 t
ORIGIN      
        1 gcttcgcttg ccgccacagt ccaaagcgac tttgtcactg gccctggggc tccctttttg
       61 atctcccttt ccgcttgtca cagtctcccg ccgctcctaa agttgcttgc tggtgaatca
      121 atacgacacg acatgattga actgattcag ttttcctgga gcccattctg catcgtccaa
      181 cggcgcatcc tcgagttcgc cggcgtccga ttcaaagtga ccaacatccc cggcaatgac
      241 cgctccctgg tctggcgatt gacccggcaa cgctattatg gcgtgccgat catccgggac
      301 ggcaaagcgg tcctcttcga ggtgagcgag gattcgcagg ttatcgccaa gtacctcgac
      361 accaagttac agcttggatt gttcccgtgg gacctggagg gcgtgcagtc cattgtctgg
      421 cgctacatcg agaatgagat cgaaggcgtc ggcttcaagc tcaacgacat ctactggcgc
      481 aaggtggtgc cgccgccgga ccagctcgcc tttctccggc acatggaacg gaagttcggg
      541 cgcggctgcc tcgaccagtg gcgggcgcaa cagaaggagc tgctgacgca actcgaacag
      601 cggttggttc cgtttgaaga aatgctcctg catcagccgt ttctcttggg ccaaaggccg
      661 cggttcgtgg acttcgacct ctatggcatc ctgggcaatt tcctttattc cggccaatac
      721 cggctgccgg ccgcgcacac tggcctgcga aagtggcacc agcgtatgac gcgcatgcaa
      781 gggcaatcaa cggcttgcga caagaaagga cactcctgac tccccaaggg gattgacgaa
      841 gaacatgaaa acactgctac cgtggctatg cgtcgtgggc ctggtcatcg ggctgggctg
      901 ggtttattcc gccggccaga agaaggacgc cgagctggcc gccttgcgcg aggagagcca
      961 acaactgcaa aagcttcgcg ccgagatgga agaaaccaag gggacccacg cgacggcgga
     1021 aagcgatgag ctggtcaagc tgcgcaaggg caacgaagac ctgctgcgcc tgcgcaatga
     1081 agtcggccaa ttgcgcaagg agaaccagca actctccggg caggtccaga cggcccaggc
     1141 gcaagtgcag ggcgcccagg ctcaagccca ggccctgagg gcgaatgcgg cgcaagcccc
     1201 cgcgccgggg cagctcgacc ctgcggctca ggccgcgttt gcggcgcgtt acgggctggc
     1261 gccccagact cccgagcaac agcaagccag cgtctgtatc aacaatctcc ggttaataga
     1321 tggggcgaag cagcagtggg ccttggagaa gcaaaagccg gcgggcgctt tgctcaccgc
     1381 cgcggacctc gcgccttatc tcaaaaccaa tacgttgccc acctgcccgg cgggcggcgt
     1441 ctataccctc aacccggtcg gcttcagccc gctttgcaac attcccggcc acgcgctggg
     1501 gaaataggct ccctgcgagc gctcagcaga tgtgctgttg cctgagctcg cggatgcgtt
     1561 cgcgccgacc cgcggcgcgc tcgaactcct gttgatcaat ggcgcggcgc agttgatcac
     1621 tgcgcctgtt tttttctcac cacatcagga aaccggcgtt acgggcttgg gttcgggcag
     1681 gagggtgcga accagccagg ccgtgaggag agtaacaatc gcgccgatga gggtgtacca
     1741 aggccagaag atttcagtgc cgacgatcct catccagatg gctttggtgc agtcgagttt
     1801 tggcaggagt tggatggcgg tcatcgtcac cagggagaca atcatgccgg tgatgaccgg
     1861 gatgctgcgg ccctgtttcc agaacacggc cagcgcgagg ccgccgacga gcgcgccgct
     1921 ggtgagtccg cgcagggcga aggcggcgtt caacacgaag gggacttggc ggctcatata
     1981 agccgtcagg atcaaaacag cggcccagac aacggtggag actttgctaa agcgcaggaa
     2041 gaagccttcg ctgcgtccgg ggaggaagtg cttcacgaag tccatggtgg agacggaagc
     2101 cagggaggtg agcgcagaac tgaccgagga catcgcggcg gacaggatgg cgacaatcag
     2161 gaatcctttc agcaggtgcg gcacttcggt catcatgaag atggggaaga tgaagtcatt
     2221 cgcggtgatg ccggagccgg ggcgggtttc gggcagcggg atctggaagg gatggccctg
     2281 gtaaaacacc cagagcatcg cgcccaccag caggaagata aagaagaggg gaaagatcac
     2341 gacggcgctc agcgccagcg ctttgcggcc atcggcgacg ctcttgcagg ccagcacccg
     2401 ttggacgatc aactgttcgg ccccgtgaga ggagagcacc accaccgtgc cgccgatgac
     2461 tcccatccag atgttgaagg gcgcgccaag gaggaacttc gtcagggaaa cgccgggtgg
     2521 cggggtgaca ttcagccagt ggagcttgcc ggcggcgagg gcttgggaga acaccgcgcc
     2581 caccccgcca tccacgagcg tcgggatgta gcagagggcg aaaacgcctc cgagcaggaa
     2641 taggccgaac tggacggcat ccgtccagat cacggccttg acgccgccga tgtaggtgta
     2701 gagcagcgag agaaccacga agagaagaat ggagccgagg atggggtcgc cggtgccgaa
     2761 gcccagaaac ttgtctccca gtatgagttt caccgggatg ctggcaacat aaacacgcac
     2821 gccggcggcc agcgtttcgc tgatgaggaa gaacgcgccg gcggtctggc gggccgaggg
     2881 gccgaaggtg ttggcgaaca gttgataggg cgagtagatc tcgcccttga aatagtgggg
     2941 aacgaggacg atggccaaaa tgatgcgggc aatgacatag ccaatgatca tctggaggaa
     3001 gacgatgttg ccgccgtaag ccatggccgg caccccgata atggtcaggg cgctggtttc
     3061 ggcggcgacg atggataagc cgatgcccca ccaggggacg gtcttgctgc cgagaaagta
     3121 gtcgcgggta ttgtgctggt ctttcccgaa ccacaccccg agaccgataa ttccaagcac
     3181 atagagcagg ataaccgccc aatcacccag gctaaagtag ctgttcatgc aatcaagagc
     3241 ccccatttag aaggagggcg agcgtcctcg cgagcccaaa ctggaagaaa tacagccgtg
     3301 taatccgcgc cggccgtggt agaaatagct tatgaagctg agcttgattg tagcggctac
     3361 gttgctcgcg gcgaattcgc ccgccgcgga ttggccccag tggcgggggc cgaatcgtaa
     3421 cggcatttct tcggagagcg tcagtgcctc ctgggctgcc gatggaccca aagtcctgtg
     3481 gcgcgcatcc gtcggcactg gcttctcttc gatctccatc agcctggggc gagcctacac
     3541 gatgggcaac acgaacaacg aggacaccgt gtggtgcttt gacgccggca ccggcaggaa
     3601 gatttggcaa cacacctatg ccgcgcaact cggcccccaa tggtacgaag gcggcccgag
     3661 ctcgactccg accatctgca gcaatcgcgt attcacaatc agcaagtggg gcgatgtctt
     3721 ctgtctcgat gccgccaagg gcacagtgct ctggcagcgg gacctgcgcc aggatggcgt
     3781 aaagcccaac cgctggggct tcgccgggtc accactgata tggggtaacc tggtgatcct
     3841 gaacgccggc accgccggca tcgcgctgga ccgcagcacg ggccgcatcg cctggtcgaa
     3901 cggcaccaac gcgggcggtt acgccagccc gacgcgcttt gtctccggcg gcaaagagtc
     3961 cgtgctcatc ttcgcggcca agcacctcgt tgccctggat ccgcagagcg ggcgcgaact
     4021 ctggcggcaa ctctgggaaa cggactggga caccaacaat acggacccgc tagtccacgg
     4081 tggcagcatc ttcatttcca gtttcaaccg tggctgcggc ctcttctctg tggcgagcgg
     4141 cataccagaa ataacctaca ccaacaaggt cctgtataac cacctcagct cgggcatcct
     4201 ggcgggcgat tatctctacg ccttcaacgg cgaggccaag aaggacacgg acttccgctg
     4261 catccacgtg cccaccggcc aactgaagtg ggcgcgcaag gatcctccgt tcggcgcgct
     4321 aatcctggcc ggggacaagc tgatcgtcct ctccgagaag ggcgaattgc tggtggggca
     4381 ggcgtcgccg gaggaattca agccgctggc gcgcgccaaa gtgctgagcg gagtttgctg
     4441 gactcctccc gcgctggctg acgggcggct ctacgttcgc aacgcccgag gcgagttgca
     4501 ctgcctggaa ttgcctcggt gacgtccacc tgggcctgac gcttgtcgaa caagaggcgg
     4561 cggaccgcca gagcggcctc gcgtcgccga gacggtcagg agaccgcaac ggggggtaga
     4621 tttcccttga ttcccttcgt aaccccttcg aagccccgtc gtaactccgt cgtaactcct
     4681 tcgaagcatc acgcgagcaa caggcgagca acaggcgagc atcacgctgg cagcgccgaa
     4741 ggatcaggcg attctcgggc ttcggatttc agggctgcat gacttggtcg cccttcaa
//
LOCUS       DATCGS010000401         5821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_183468, whole genome shotgun sequence.
ACCESSION   DATCGS010000401 DATCGS010000000
VERSION     DATCGS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183468"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIL39_08190"
     CDS             <1..339
                     /locus_tag="VIL39_08190"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481443.1"
                     /translation="GNIILNDEGWEIRTEKKPANLDNQRKPGSPDPRPAHVRNFLACV
                     KSRQQPVLNLDIGHHVSSVAHLGNIAYRTGRKIVWDAANEKIVNDHEADKLVGVKYRQ
                     PWKLPYARRA"
     gene            complement(360..560)
                     /locus_tag="VIL39_08195"
     CDS             complement(360..560)
                     /locus_tag="VIL39_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481444.1"
                     /translation="METRHKTAIWGVAAVIAFVALAFLSPPIPRTKARASRVSGVNNV
                     RSVSLVITNTSALPSGQPGTGK"
     gene            complement(575..1384)
                     /locus_tag="VIL39_08200"
     CDS             complement(575..1384)
                     /locus_tag="VIL39_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481445.1"
                     /translation="MAQPSQARVPQLATLPGREGKGGYCPGRAFTLLELLLVIAIIAI
                     LAALLVPALAAAQAKGKRVACLDNLKQSALSFQMYTADNDGKLAQNYPLVQAGTDSWV
                     LGDMKLSGDSTNKTLIRQGKFYPYASQVAQYRCPADPSRTGDALRVRSYSMNGWIGSR
                     YMETYPGTSGFRTFVRDSEVAAAGPAKLWVIIDEHEASIDDAWFLVTMDDSKPFASFP
                     AARHEGSYGLNFADGHTEFYKLRESEFSGPKPEVSVNNSDWQRLKQVTTVR"
     gene            1523..4633
                     /locus_tag="VIL39_08205"
     CDS             1523..4633
                     /locus_tag="VIL39_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481446.1"
                     /translation="MTPKGWMLLLGAALLMPLETSWGQKPSNWPVNWRAHRIADGLPE
                     SACISVALAPQGKVLARHLKSGSVSELDGYTVSVIPSPGPGNGRVYQSPGGQLWAVVA
                     EGLQEFKNGQWMLHPVPEVSAAFRARLPRVIDPIPLCPARQSVVVFLLPDRLLQFNAE
                     DPDRPRTEVLLTAAQTQLERFSSMTLARDGGLWIAGARGLLKVPGPVRNLKPGMEWHD
                     YAPPAPLSIRDLQEPHEEDGGIVTALAEAAGRNQKMLVHFDGQHWTPEAIPVERVRHA
                     WCGPDKTCWGATIDSLFAWPEGGREVGESDESLARQYYDVAVEPGGAFWLATSDGLFR
                     YAPLTWRSPAYVHQINSLVHGLAEDEAGRLWFISGAGLHALQNERHQEYSLPASISNS
                     VPGGLALFPLKNGALLLQAGDQCLQFHTDSGAFSAVLPEPPAMLLKPLGLLKDGRLCV
                     QIPSGEAPAQRYRLETYDGATFEPLSVPPPEPSLGPGLSTLFVAQNGDLWVSGDHGTA
                     CFHDQKWRTFTSTDKSSPESVVGFAELPDGRIWGATPDKVWAFDGRSWSAMRVGMDRI
                     NAFIGTRDGSLWVAGDSGLHRYFQGAWVENGTEEGLPGGGIRDLYEDQRGRVWAGTAS
                     GLSLYHPEADPDPPRTSTQTLTDKEKTIPTGGTITLTFNAQDKWKYTSRQRLLFSCRL
                     DGRDWSTFQEDRSVSFTDLPAGKHYFQVRAMDRNCNIDPSPARLEFVVILPWYKESRP
                     VLIAFAGLAVALFFAGLAFNRHRQLLRSYAEVEQKVAQRTRELEIANRQLLHSQKMNA
                     LGTLAAGIAHDFNNILSIIKGSAQIIEDNLDNPQKVSTRVDRIKTVVEQGAGIVKAML
                     GFSRDSGDQPALCDLNAVLDDTVKLLGDRFLREVRLTLERATDLPAVSCSKDLIQQVL
                     LNFIFNAAESMTSRKRIILATRQLDKLPASLVLAPAQAARYAAISVQDFGTGISPDNL
                     PRIFEPFFTTKALSVRRGTGLGLSMVYELAKKMEAGLAVESVVDHGSTFTLILPVRKP
                     DAHPAS"
     gene            5055..5699
                     /locus_tag="VIL39_08210"
     CDS             5055..5699
                     /locus_tag="VIL39_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481447.1"
                     /translation="MNPSTILIIEDDDLQYEIYEEALAKYKLLRATNGTEALAQIPKA
                     PPDVLILDHVLSDGELGLDFLPEFKDLLPFVPIIIVSGALEVHQQMEALQGPRRAHYC
                     LTKPVDIRTLKHTVETALKECGEREAIRQFEALERSHRMDALDLFSRSTDRLSRQNRI
                     HELVGKSRQRPNISALARQFGVARRTIIRDLQELIRRGQLKPEVYPEWENPPEE"
BASE COUNT         1099 a         1983 c         1676 g         1063 t
ORIGIN      
        1 ggcaatatta tcctcaacga cgagggctgg gaaatccgca ccgagaagaa accggccaac
       61 ctcgacaacc agcgcaagcc tggcagcccc gacccgcgtc cagcgcatgt gcgcaacttc
      121 ctcgcttgcg tgaaatcgcg ccagcaaccg gtgctcaacc ttgacatcgg ccatcacgtc
      181 tccagcgtgg cgcacctcgg caacattgcc tatcgcaccg gccgcaagat agtctgggat
      241 gccgcgaacg agaagatcgt gaatgaccat gaggccgaca agctggtcgg cgtcaagtat
      301 cgccagccgt ggaagctgcc ctacgcccgg cgggcgtagc aaatcacgaa cgcgactgtc
      361 tatttgcccg tgcccggctg gccgctgggc agcgcgctgg tattcgttat gaccagggag
      421 acactacgga cattgttcac ccccgaaacc cgacttgctc gggccttggt ccgggggatg
      481 ggcggcgaca ggaaagccag cgcgacgaat gctatcaccg cggccactcc ccagattgca
      541 gtcttgtgtc ttgtttccat aacgggcgtt cctttcaccg gacggtcgtc acctgcttga
      601 gcctttgcca gtcagaattg ttcacgctga cttccggctt cgggccggag aattctgact
      661 cccgcagctt gtagaactca gtgtggccat ccgcgaagtt cagtccgtag ctgccttcat
      721 ggcgtgccgc gggaaagctg gcgaacggct tgctgtcatc catggtcacc aggaaccacg
      781 cgtcgtcaat gctggcttcg tgttcatcaa tgatcaccca aagcttagcc ggccccgccg
      841 ccgccacctc gctgtcgcgg acgaaggtgc gaaagccgct ggtgcccgga taggtctcca
      901 tgtagcggct gccgatccat ccgttcatcg aatagctgcg gacccgaagc gcatcccccg
      961 tgcgggaagg atcggctgga caccgatact gggccacttg gctggcgtag ggatagaact
     1021 tcccctggcg gatgagggtc ttgttcgtcg aatcgcctga aagcttcata tccccgagca
     1081 cccaggagtc ggtgccggct tgcacaaggg gataattctg cgccagcttg ccgtcgttgt
     1141 ccgccgtgta catctggaac gagagggcgg attgcttgag attatccagg cacgcgactc
     1201 gtttgccctt ggcctgcgcg gcggcgagcg ccggcacgag cagggccgcc aggatggcaa
     1261 taatggcgat cacgagcagc agttcaagca gggtgaacgc ccggcccggg caataccccc
     1321 ccttgccctc gcgacccggc agagtcgcga gctgcggaac ccgtgcttgt gatggctgag
     1381 ccatgaactg cttcatcccc atcataaccg acgccggccg ctcttgagaa gtgtgcgttt
     1441 gacggcacac ccccggcttt tgattgcacc gccaggccag ctagtgaata ctcccggcgc
     1501 gatcaaaact gtcacggcat ctatgacccc caagggctgg atgctcctcc tcggcgcggc
     1561 gctgctcatg ccgttggaga cgagctgggg ccagaagccc tcgaattggc cagttaattg
     1621 gcgcgctcac cgcatcgccg acggcttgcc cgagtccgct tgcatctcgg ttgcgctggc
     1681 cccgcagggg aaggttctgg cccgccacct caaatcaggg tcggtcagcg agctcgatgg
     1741 ttacaccgtc agcgtcatcc cctcccccgg tcccggcaac ggtcgcgttt accagagtcc
     1801 cggcggccag ctctgggcgg tcgtcgcgga gggcctgcaa gaattcaaga acggccagtg
     1861 gatgcttcat ccggtcccgg aggtctccgc cgccttccgc gcccgcctcc cgcgcgtgat
     1921 tgatcccata ccgttgtgcc cggcccggca gagtgtcgtc gtcttcctgc tgccggaccg
     1981 cctgttgcag tttaatgccg aggaccccga ccgtccccgc accgaggtgc tgctgactgc
     2041 cgcgcagaca cagcttgaaa ggttctccag tatgactctg gcccgcgatg gcggactgtg
     2101 gatcgccggc gcgcgcggac tgctcaaagt cccgggtcca gttcgcaatc tgaagccggg
     2161 aatggagtgg cacgattacg ctccacccgc gccgctctca attcgcgatc tgcaggagcc
     2221 gcacgaagag gatgggggca tcgttaccgc tttggctgaa gctgcgggga ggaaccagaa
     2281 gatgctggtc catttcgacg gccagcactg gacgcccgag gccatccccg tcgaaagggt
     2341 caggcacgcc tggtgcggcc cggacaagac ctgctggggg gcgaccattg actccttgtt
     2401 cgcttggccg gaaggcgggc gggaggtggg tgagagcgac gaaagtctcg cgcgccaata
     2461 ctacgacgtg gccgtcgagc ctggcggcgc cttctggctg gcgacctccg acgggctgtt
     2521 tcgctatgcc ccgctcacct ggagaagccc cgcttacgtc caccagatca attccctggt
     2581 ccacggtctg gcggaggacg aggctggccg gctgtggttc atctcaggcg ccgggcttca
     2641 tgctctccag aacgagcggc accaggagta ttcattaccg gcctcgatct caaacagcgt
     2701 cccaggcggg ctcgctctgt tccccttgaa gaatggtgcg ctgttgctcc aagcgggcga
     2761 ccaatgcctg cagtttcata ctgatagcgg cgcattcagc gcggttctgc ctgaacctcc
     2821 cgcaatgctg ctcaagccgc tggggttatt gaaggatggc agattgtgcg tccagattcc
     2881 cagcggcgaa gcccccgcgc agcgctaccg cctggagacg tacgatgggg ctacattcga
     2941 gccgctgtcc gtgccgccgc ccgagccttc gctgggcccc ggtctctcaa cgctgttcgt
     3001 cgctcagaac ggcgaccttt gggtaagcgg cgatcatggc acggcctgct tccacgatca
     3061 gaagtggcgg acgttcacct cgaccgacaa gagcagtcct gaatccgtag tcggcttcgc
     3121 tgagcttccg gacggcagga tctggggcgc taccccggac aaggtctggg cgttcgacgg
     3181 tcggagttgg tcggcgatgc gcgtgggaat ggaccgcatc aacgccttca tcgggacccg
     3241 tgacggcagc ctgtgggttg ccggcgacag cgggttgcat cgctatttcc aaggcgcctg
     3301 ggttgagaac ggcacggaag agggtctgcc cgggggcggc atccgggatc tctacgaaga
     3361 tcagcgcggc cgggtctggg cgggcaccgc cagcgggttg agcctctacc acccagaagc
     3421 agacccggac ccgccgcgga cttccaccca aacgttgacg gacaaggaga agaccatccc
     3481 gaccggcggc accatcaccc taacgttcaa cgcgcaggac aagtggaaat atacatcccg
     3541 ccaacgcctg ctcttctcct gccgtctgga cgggcgcgac tggtccactt ttcaagagga
     3601 caggagcgtg tcctttaccg acctgcctgc cggcaagcac tacttccagg tccgcgccat
     3661 ggaccgcaac tgcaacatag acccctcccc tgcccggctc gagttcgttg tcatcctgcc
     3721 ctggtacaaa gagtcgcgcc cggtcctgat tgcctttgcc gggctggctg tcgcgctgtt
     3781 cttcgccggc ctggccttca accgccaccg ccaactgctc cgcagctacg ctgaagtcga
     3841 acagaaagtc gcccagcgca cgcgggaact ggagatcgcc aaccgccaac tattacacag
     3901 ccagaagatg aatgccctcg gcaccctcgc cgcaggcatc gcccacgact tcaacaacat
     3961 cctttccatc attaaaggtt ccgcccagat catcgaggac aacctcgaca atccccagaa
     4021 ggtcagcaca cgcgtggacc gcatcaagac cgttgtcgag cagggcgccg gcattgtcaa
     4081 agccatgctc ggcttcagcc gcgactccgg agaccaaccc gccctctgcg acctcaatgc
     4141 cgtcctggac gacaccgtca agctgctcgg cgaccggttc ctccgcgagg tgcggctcac
     4201 cctcgaacgc gccaccgacc tgcccgcagt atcctgctcc aaggacctca tccagcaagt
     4261 cctgctgaac ttcatcttca acgccgccga atccatgacc agccgaaagc ggattatcct
     4321 ggccacgcgg caactggaca agctgcctgc gagcctggtg ttggcgcccg cgcaggccgc
     4381 ccgctacgcc gccatttccg tgcaggattt cggaaccggc atctcccccg acaacctgcc
     4441 ccgcatcttc gagcccttct tcaccaccaa agccctctcc gtgcggcgcg gcaccgggct
     4501 gggcctctcg atggtctatg aactggccaa gaagatggaa gccggcctcg ccgtggagtc
     4561 cgtcgtggac cacggcagca cgtttacgct tatcctgccc gtgcggaagc cagatgcgca
     4621 cccagcgtct tgagccatga gtgggtgcaa accagaactc cggagttcga gagctatgtg
     4681 caaactcgcc gcgcttcgtg ggtttggaag ccccattccc cctcacccca gccctctccc
     4741 caaggagagg gagcaccgtc gcctacgtgt ccgccgatcc acaacgcttg agaatgtcgc
     4801 agcctggcca cggggtctcc ctctccctaa gggagagggc tgggctgagg gggaacaggg
     4861 cgtgccacta ccggagagcg aactcttttc ccgtcgcctc acaataaccg cctccccaca
     4921 tcggctccca gcaaggcaga ccgcgctcgc ttctgcccag gacgaatcat tcggatttcg
     4981 gatttcggcc ttccttcggc cttcggattt cggccctcgg atttctcctc tccactattc
     5041 ccgcacaccc tgacatgaac ccttccacca tcctgatcat cgaagacgac gacctccaat
     5101 atgagattta cgaagaggcc ctcgccaaat acaaattgct tcgcgccacc aacggcaccg
     5161 aagccctcgc ccagatcccc aaggccccgc ccgacgttct gatccttgac cacgtcctct
     5221 ccgatggcga gctcggcctg gatttcctcc ccgagttcaa ggacctgctg ccgtttgttc
     5281 ccatcatcat cgtctcgggc gccctggaag tgcaccaaca aatggaggca ctccagggac
     5341 cccgccgcgc ccattattgc ctcaccaaac cggtggacat tcgcaccctc aagcacaccg
     5401 tcgagactgc cctcaaagaa tgcggcgagc gtgaagccat ccgccaattc gaggccctcg
     5461 aacgctccca ccgcatggat gccctcgacc tgttcagccg ctccaccgac cgcctcagcc
     5521 ggcagaatcg tatccatgaa ctcgtcggca aatcccgcca gcgccccaac atctccgccc
     5581 tcgcccgcca gtttggcgtt gcccgccgca ccatcatcag agacctgcag gaattgattc
     5641 gccgcggcca gctcaaaccc gaagtctatc ccgagtggga aaatccgccc gaagaatagt
     5701 agtctcatag catacggtgg ctcttgaggg agaagggcac ctgaacaggc acaagcggca
     5761 cccctttaag ataccggagt gggtgcctat cgcctcccaa gcaacacgct tgtgggtggc
     5821 t
//
LOCUS       DATCGS010000402         4259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_726906, whole genome shotgun sequence.
ACCESSION   DATCGS010000402 DATCGS010000000
VERSION     DATCGS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726906"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1606)
                     /locus_tag="VIL39_08215"
     CDS             complement(77..1606)
                     /locus_tag="VIL39_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481448.1"
                     /translation="MTANALAAVELRGLTKQFGSVMANAGVNLRVEAGTIHGVIGENG
                     AGKSTAMKILYGMLRPDAGEILVGGRKCVWASPSDAIAQGIGMVHQHFMLAGPYSALD
                     NILLGAEPLRWRVIDRQGARARLEALARQYGLPVEWDRPIEQLPVGVQQRVEILKLLY
                     REANILILDEPTAVLTPQETNDLFGNLKKLRAEGKTVLLVTHKLKDVMSFTDRVTVFR
                     AGKVTGEVETSHTSPQELANLMVGRKVVLNIEVPPAHPRAEPAIEVAGLSLAGPAGSR
                     HRLSDLSFSVRRGEIVGVAGVEGNGQSELLQAMLHPRDSQCRTSGTIRFVGEDVTAWA
                     PSRIRDLGVAAIPEDRLQEGLLLDRPVSENFLLGLQRSSAFSRAGFLNPRDLAQTAAR
                     AIADYDVRPPDLTLPAGKLSGGNQQKLIVAREFQRQPRVLIAAQPTRGVDVGAIEFIH
                     TRIVRARDEGAGVLLVSSELDEILTLSDRILVMFDGRIVAEFRRGEVTERELGLKMGG
                     A"
     gene            complement(1672..3192)
                     /gene="rpoN"
                     /locus_tag="VIL39_08220"
     CDS             complement(1672..3192)
                     /gene="rpoN"
                     /locus_tag="VIL39_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY5481449.1"
                     /translation="MPQGLHLSQRLTQSLVLAPQLQQSLALLQAPTLELKALVEQELQ
                     QNPVLEEESAAETEQQEKDGRETEAPAEAADPSEPPADLSFDSTTEKSAGEPVDEFQA
                     EFNRLVQLDQEWRDHFAQTNLPMRASAEDEEKRQFMFDSLVAGTSLQEMLLEQVREST
                     LAEDQWPVAEMVIGNIDEYGYLKVTVEELVATTGLPVDQIAGVVKVIQSFDPPGVGAR
                     DLRECLLLQLDRLGQQETLEYRIINDFMEALGKRRIPEISRDTGTSVDEVQEALERIA
                     RLEPRPGRAYLPDNDQYILPEVFVRRSGDDYVVTTNNEHIPHLRISNTYKDLMSQGGN
                     SAEVRNYIREKIRAGKFLIKSLHQRQQTILNIANEIVHRQREFMSKGVAALKPLTMVQ
                     VAEVVGVHETTVSRAVSGKYMQTPQGIFEMKYFFTGGIQTANANGDGMSSTSMSNTSV
                     KDIIAKIFKAEATSKPLSDQEVVRMLKEKGIVIARRTVAKYRTELNILPSNLRKVY"
     gene            complement(3363..>4259)
                     /locus_tag="VIL39_08225"
     CDS             complement(3363..>4259)
                     /locus_tag="VIL39_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481450.1"
                     /translation="EVVANMTKMLGRILGEDIELQLIYSPHPARVQADAGMMEQVLLN
                     LAVNSRDAMPRGGLLAIKISALEVDARRLAEHPEARAGQFVCLRVTDTGCGIPPEILR
                     RIFEPFFTTKEVGKGTGLGLATVYGIVKQHQGWIEVESEPGKGTTFSIFLPRSLDAAP
                     VAGQRPAEPAARGGTETILVVEDEAPVRELVCHLLAEHGYQILQAESGAKALQVWRES
                     KDRISLLLTDLVMPDQMNGHELAEKLRAEQPRLKVIITSGYSADVVGKDFILRPGLNY
                     LQKPYPPHKLALAVRACLDAVN"
BASE COUNT          739 a         1379 c         1258 g          883 t
ORIGIN      
        1 tggtcatgca tgccttttgt tttggcgccg gacactgtcc ccaagttcgg ggccagtgcc
       61 tgagtgcgtc ggttcttcac gctcccccca tctttagccc cagctcgcgc tcggtcacct
      121 cgccgcggcg gaattcggcc acaatccggc cgtcaaacat cactaggatg cggtcggata
      181 acgtaagtat ctcgtccagt tccgaggaga ccagcaagac cccggcgcct tcgtcgcggg
      241 cccggacgat gcgggtgtga atgaactcaa tcgccccgac gtccaccccg cgcgtgggct
      301 gcgccgcgat cagcacccgc ggctggcgct ggaattctcg cgcgacgatg agcttttgtt
      361 ggttgccgcc ggagagcttt ccggcgggca gggtcaagtc cggcgggcgc acgtcgtaat
      421 ccgcgatcgc acgcgccgcc gtttgggcca ggtctcttgg gttgaggaaa cccgcacggc
      481 tgaaggcaga actccgctgg aggcccaata ggaaattctc gctcactggc cggtccagca
      541 gcagcccttc ctgcaaacga tcttccggga tggcggcgac tcccaggtcg cggattcttg
      601 acggggccca ggcagtaaca tcttcaccca cgaaccggat ggtcccggag gtcctgcatt
      661 gggagtcacg cggatgcagc atcgcttgca gcagctccga ctggccattc ccttcgacac
      721 cggcaacgcc cacgatttca ccgcgcctga ctgagaagct gaggtcggac agccggtgac
      781 ggctgcccgc cggccctgcc aggctcaagc ccgcaacttc gatcgcgggt tcagcgcgcg
      841 ggtgcgcggg cgggacctca atattcagca ccacctttcg cccgaccatc aggttggcga
      901 gttcttgcgg actggtatgg gaagtctcca cctcgccggt cactttgccg gcgcgaaaca
      961 ccgtgacgcg atccgtgaag gacatcacgt ccttgagctt gtgggtgacc agcagcacgg
     1021 tttttccctc agcgcggagc ttcttgaggt tgccaaagag atcattcgtc tcctgcgggg
     1081 tcagcaccgc cgtcggctca tccaggatga ggatgttcgc ctcgcgataa agcagcttaa
     1141 ggatttcgac gcgctgctgc acgccgacgg gaagctgctc gatgggacgg tcccactcga
     1201 ctggcagccc gtattggcgc gccagggcct ccaggcgggc gcgggctccc tggcgatcaa
     1261 tcactcgcca acgcagcggc tcggccccca gcagaatgtt atccagcgcc gagtagggac
     1321 cggccagcat gaaatgctgg tgcaccatgc cgatgccctg ggcgatggca tccgagggcg
     1381 acgcccaaac gcacttcctt ccgccgacga gaatctcccc ggcgtccggg cgcaacatgc
     1441 cgtaaaggat cttcatcgcc gtagatttcc ctgcgccgtt ttccccgatg acgccatgaa
     1501 tcgtgcccgc ctcgacccgc agattgaccc ccgcgttggc catgacgctc ccgaactgct
     1561 tcgtcagtcc gcgcaactcg actgctgcaa gggcattggc cgtcatgacc ttggtagtgt
     1621 ctgaatctga gcgcgcgagt caacgttcag gatctgatca acgtggtggg ttcagtaaac
     1681 cttccgcaaa ttggacggca ggatgttcag ctcggtgcga tacttggcca ccgtgcgccg
     1741 ggcgatcaca atgccttttt ccttgagcat ccggaccacc tcctgatccg agagcggctt
     1801 gctggtagct tccgctttga atattttcgc aatgatgtcc ttcacactgg tgttggacat
     1861 gctggtggag gacatgccgt cgccgttggc gttggcagtc tggatgccac cggtaaagaa
     1921 atacttcatc tcgaaaatcc cctgcggcgt ctgcatatac ttgccggaga ccgcgcggct
     1981 gacggtggtt tcatgcacgc cgaccacctc cgccacttgc accatcgtga gcggtttgag
     2041 cgcggcgacg cctttgctca tgaactcgcg ctgacggtgc acgatctcgt tggcgatgtt
     2101 caggatggtc tgctgccgct gatgcaagct cttgatcagg aacttcccgg cgcggatctt
     2161 ttcccgaata taattccgca cttcggccga gttgccgcct tgggacatta gatccttgta
     2221 ggtgttgctg atgcgcaagt gcgggatatg ctcattgttg gtcgtgacca cataatcgtc
     2281 cccggaccgg cgcacgaaca cctccggcag gatgtattgg tcgttatcgg gcaaataggc
     2341 gcgtccaggc cgcggctcca accgggcgat gcgctccagc gcctcctgca cctcgtccac
     2401 cgaagtgccg gtgtcgcggg agatctccgg gatgcgccgc ttgccgagcg cctccatgaa
     2461 gtcgttgatg atgcggtatt ccagcgtttc ctgctggccc aggcgatcaa gctggagcag
     2521 caggcattcc cgcaaatcgc gcgcgccgac gcccggtggg tcgaagcttt gaatcacttt
     2581 caccactccg gcgatctgat ccaccggcag ccccgtggtt gccaccagct cttccacggt
     2641 gactttcagg tagccgtact catcaatgtt gccgatcacc atctccgcaa ccggccactg
     2701 atcctccgcc aaggtggact cgcgaacttg ctcgagcagc atttcctgca gcgaggttcc
     2761 cgccaccaac gaatcgaaca tgaattgtcg cttctcctcg tcctcggcgg aggcccgcat
     2821 cgggagattg gtttgggcaa aatgatcgcg ccattcctgg tcgagctgca ccaggcggtt
     2881 aaactccgct tgaaattcat ccaccggttc gccggcgctc ttctcggtgg tggagtcaaa
     2941 gcttaaatcg gcggggggtt cgctcgggtc agccgcttcg gccggcgctt ccgtttcgcg
     3001 accgtctttc tcctgctgct ccgtttccgc cgcggactcc tcttccagca ccgggttctg
     3061 ctgcaattcc tgttcgacca gcgctttgag ctccagcgtc ggcgcttgca agagggccaa
     3121 cgactgctgt agttgcggtg ccagcaccag cgactgcgtc aaacgctgcg acagatgtaa
     3181 gccttgaggc atggcgaaat cttaagccgc catggaacca acacaagcaa gaacttggca
     3241 cgactttggc tgtcggcgca tctggtactg gcgccgccgt ctcggccgaa ggcccggagc
     3301 gctggaatcc agcgttcccg gggccgtgtc tgcctgcgcc ctttggcttg tcgccgccct
     3361 cttcagttca ccgcgtccag gcaagcgcgt actgccaggg caagtttgtg gggagggtac
     3421 ggcttctgga gataattcaa accggggcgc aagataaagt ctttgccgac cacgtccgcg
     3481 ctgtaaccgc tggtaatgat tactttgagc cgcggttgct ccgcccgcag cttttccgcc
     3541 agctcgtgcc cgttcatctg gtcgggcatg accagatcgg tcagcagcaa gctgatccgg
     3601 tccttgcttt cccgccagac ctgtagcgct ttcgcgcccg actcagcttg caggatttgg
     3661 tagccgtgct ccgccaggag atggcaaacc agctcgcgca cgggcgcttc gtcctcaacc
     3721 acgaggatgg tttcggtgcc gccgcgagca gccggttcgg ccggccgctg acccgcaacc
     3781 ggggcagcat ccaggctgcg cggcaggaat atgctgaagg tcgtgccttt gccgggttcg
     3841 ctctcaacct caatccaccc ctgatgctgt ttgacgatgc cgtacaccgt cgccaagccc
     3901 aaacccgttc ccttgccgac ttccttggtg gtgaagaacg gctcaaagat ccggcgcaga
     3961 atctccggcg gaatgccgca gccggtgtcg gttaccctca ggcaaacgaa ttggccggcg
     4021 cgcgcttccg ggtgttccgc caggcggcgg gcgtccacct ccagcgctga gatcttgatc
     4081 gccagcaggc cgcccctggg cattgcgtca cgagagttga cggccaggtt caacaacact
     4141 tgttccatca tgcccgcatc ggcttgcacc cgcgccgggt gtggggaata aatcagttgc
     4201 agctcgatgt cttccccaag aatgcgtccc agcatcttgg tcatattggc gaccacctc
//
LOCUS       DATCGS010000403         3763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_628092, whole genome shotgun sequence.
ACCESSION   DATCGS010000403 DATCGS010000000
VERSION     DATCGS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628092"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..2499)
                     /locus_tag="VIL39_08230"
     CDS             complement(271..2499)
                     /locus_tag="VIL39_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481451.1"
                     /translation="MKSRTLFKNELIGWPWAALFGLWVAASTLWAAETNAPAGAITNA
                     PPATATNTPPSTVTNAPSATATNAPKQTVAAPLTPEQMFEGGTNAYNNWIEFGVGGFF
                     TSGNKAQFQQRNQTSGGAFGGIEDFHCQNTIAKGATMNVDGRGIFDNDDYKLSLAVDK
                     EKLGYVRFSVTQFRTWSDGDGGFYAPANVYYPQGENALALDRGDISIEAGLTLDKLPQ
                     VKFKYDHLYRDGEEGSTSWGYTHPVGGTLVRGLSPSIYDIDERSDVFQLDVTHHIKAT
                     EFGAGLRYESGRLDDALKIDQFPGESFQQKITDRQGTTYDLFNVHSFTESWINKNVLL
                     SSGFSYSDLDNTFSGSRNYGSDYDVVYTPYSQNGVGYYGLSGGSHLNEYVGDLNLFIK
                     PWKHFTIVPSLRIQQQDTDANSGGFETMGAYSPASFTANSGQSDLDVRERLDLTYNGI
                     TNWVLYARGELTEGSGNLAENGGLGPINGIGVPPIQRYTDDDRFFQKYSAGARWYPAR
                     RLTVDAGGYYKHNDYNYDHTLDSTPNDSASPNQYPAYLVMQNFETYDGNIRVTLRPRQ
                     NVTAVTRYDYQYSTIQNRPDPISGLSEMESSRTTSHIFAQDVSWTPWSRLYLQAGFNY
                     ILNQTRTPASDSTQAVLPAQNDYWTLNFSPGLVLDDRTDLKVSFFYYQADNYQDNSLY
                     GVPYGAGDEEYAITATLTRRINRHLRVALKYGYSHYKDQTYGGHRDFDAQLVYTSMQY
                     RF"
     gene            complement(2517..3377)
                     /locus_tag="VIL39_08235"
     CDS             complement(2517..3377)
                     /locus_tag="VIL39_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5481452.1"
                     /translation="MMKCNFRLPGGQHAKWKRLLAVTGGAIVLLLAVISCRTVNRAVV
                     VLPDVPGAKYIGSKECEQCHDKLCRDFATADHARLIAEGPNALNAGCESCHGPGSLHS
                     ESGGEVKPPYSFTPGRPQVSSYGARPAVQPTRATETVCFQCHPNIRGQFQLPSHHPVP
                     EGRLSCTACHSPHKGSIYRTGGTSLASANQGCLGCHPAQRGPYVFEHEALREGCTICH
                     TPHGSVNAKLLTVRDANLCLKCHFQQIRGGQLLIGGSDHTLRLQQGTCWTGGCHEAVH
                     GSRVTSSLRF"
     gene            complement(3453..>3763)
                     /locus_tag="VIL39_08240"
     CDS             complement(3453..>3763)
                     /locus_tag="VIL39_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481453.1"
                     /translation="VITSQINEVKASLDQWATTKAPPSLTAKYGKRAWEYTTPGALSP
                     GGSGPNAAGQALIPANIKKARFNLYLVLYDGSFGVHNGPYSVTLLDTAQNWVDQELSR
                     "
BASE COUNT          747 a         1063 c         1143 g          810 t
ORIGIN      
        1 ccccttccgt gtcaaggaag tgctctacca ctgagctaac cgcgccacca gaatcgacgc
       61 ccgttaagtg cttcatcttg agcgtcatgc ggagagtatc cgcaagccgc gcagcacgcg
      121 caagtcgatt ctgaatgccg ccgttagaca aaatgttaga cagtcaggaa gtgatctgcg
      181 tggccgcgca cttcccgttg gttgcgcatg gtcccgtcgt ctgcgcgtgg aacccggtgg
      241 caaatcggat cggcagatga tgctgacgaa tcagaaccgg tattgcatgc tggtgtacac
      301 gagttgtgcg tcgaaatctc tgtgtccgcc gtatgtctgg tccttgtagt gggaataccc
      361 atactttaat gccacgcgca gatgccggtt gatccggcga gtcagcgtag ccgttatggc
      421 atattcttca tccccggccc catagggcac tccatagagt gagttgtcct ggtaattgtc
      481 ggcctgataa tagaagaagc tcaccttcag gtccgtccgg tcgtccagga ccagccctgg
      541 cgaaaagttc agggtccagt agtcgttttg cgctgggagc acagcttggg tggaatctga
      601 agctggggtt cgggtttgat tcaagatgta gttgaagccg gcctgcaagt acaggcggga
      661 ccagggcgtc caactgacat cctgggcaaa aatgtgactc gtcgtcctcg aggactccat
      721 ctcgctaagg cccgagatcg ggtccggcct gttctggata gtggagtact gataatcgta
      781 gcgagtgacc gcggtcacgt tctggcgcgg cctgagcgtc accctaatgt tcccgtcgta
      841 cgtctcgaag ttttgcatga ccaggtatgc gggatattgg ttggggctgg cgctgtcatt
      901 gggagtgctg tccagggtgt gatcataatt gtaatcgttg tgcttgtagt aaccacccgc
      961 atccacggtg aggcggcgag cgggatacca cctggccccg gcgctgtatt tctggaagaa
     1021 ccgatcatcg tccgtatagc gctggatagg cggcacgcca attccattga tcggcccaag
     1081 ccctccgttc tccgccaggt ttccactccc ctccgtcaat tcgccgcggg catagagcac
     1141 ccagttggtg atgccgttat aagtcagatc caggcgctcc cgcacgtcaa gatcgctctg
     1201 gccgctgttc gcggtgaacg aggcgggcga gtaagccccc atggtctcga atccaccgga
     1261 gtttgcatcg gtatcctgct gctggatgcg caaagagggc acgatagtga aatgcttcca
     1321 gggtttgatg aaaaggttca gatccccaac gtattcgttt aagtgggatc ctccgctcag
     1381 gccatagtag ccgacgccgt tttgggaata tggcgtgtaa acgacatcat agtctgaccc
     1441 gtaattgcgg ctgccggaga aagtgttgtc caaatccgag taggagaaac cggaggagag
     1501 cagcacgttc ttgttgatcc agctttcggt gaaggagtgg acattgaaga ggtcataagt
     1561 ggtgccttgc cggtcggtga tcttttgctg gaaggattcg ccggggaatt gatcaatctt
     1621 cagcgcatcg tccaaccggc ctgactcgta gcgaagcccc gcgccaaact cagtggcttt
     1681 gatgtggtgg gtcacatcga gctgaaaaac atcgctacgc tcatcgatgt catagatcga
     1741 ggggctgagc ccgcgcacca aagtgccacc cacaggatgg gtgtatcccc aactggtcga
     1801 accttcttct ccgtcccggt agaggtgatc atatttgaac ttaacttgcg gcagtttatc
     1861 cagggtcaac ccggcctcga tggagatatc gccccgatcc agcgccagcg cgttttcccc
     1921 ctgcggatag taaacgttcg ccggcgcgta aaagccgcca tccccatcgg accaggtgcg
     1981 gaattgggtg accgagaaac gcacataccc tagcttctcc ttatccaccg ccaggctcag
     2041 tttgtagtcg tcattatcga agatgccccg tccgtccacg ttcatcgtgg cgcccttggc
     2101 gatggtgttc tggcagtgga aatcctcgat gccgccaaag gccccgcccg aggtctggtt
     2161 tcgctgctgg aactgggcct tgttgccgct cgtgaagaag ccgccgaccc cgaactcgat
     2221 ccagttgttg taggcattgg tgccgccctc aaacatctgc tccggggtca gcggggcggc
     2281 aacggtctgc ttcggggcat tggtagcggt cgcggatggg gcattcgtaa cggtgctcgg
     2341 cggcgtattg gttgcagtcg ctggcggggc gttcgtaatg gcccccgccg gagcattggt
     2401 ctcagccgcc caaagagtgg aggcggccac ccagagaccg aacaaagcgg cccaaggcca
     2461 cccaatcagc tcattcttga acagggttct tgatttcatc gggtttcgag ctgtggttag
     2521 aaacgcagag aggaggtgac gcgggaacca tggaccgctt cgtggcaacc cccggtccag
     2581 caggtgcctt gctggaggcg cagggtgtgg tccgatccgc caatgagaag ctggccgccg
     2641 cgaatctgct ggaagtggca tttcaagcag aggttcgcgt cgcgaaccgt cagcagtttg
     2701 gcgtttacag aaccgtgagg ggtatggcag atagtgcatc cttcgcgcag ggcttcgtgt
     2761 tcaaacacat aaggaccgcg ttgtgcggga tggcagccaa ggcagccttg gtttgcggaa
     2821 gccaacgagg tgccgccagt ccggtaaatg gaacctttgt gcggcgaatg gcaggcggtg
     2881 caactcagcc gcccctccgg caccggatga tgggagggta actgaaactg gccccggata
     2941 ttaggatggc actggaagca gacggtttcc gtggcgcgag tgggctggac agccggccgc
     3001 gcaccgtagc tgctcacctg ggggcgccca ggggtgaagc tgtagggcgg ctttacttcg
     3061 ccaccagact cagagtgaag actgccgggt ccgtggcagg actcgcaccc ggcattgagc
     3121 gcgttgggac cttcggcgat caggcgcgcg tgatcagccg ttgcgaagtc ccggcatagt
     3181 ttgtcatggc actgttcaca ctccttggaa ccgatatact tcgccccggg aacatccggc
     3241 aggaccacca cggcgcggtt gacggtgcgg caggaaatga ccgccaggag caagactatc
     3301 gccccacccg tcaccgccag caatcgtttc catttcgcgt gctgcccgcc aggcaggcgg
     3361 aagttgcatt tcatcatata agctctgact gtcgtcaatc cccgggaccc ctcggctcgg
     3421 ccagcaaccg gccgtccttc atgccgagcg cctcaccggc tcaactcttg gtcaacccag
     3481 ttctgggctg tgtctaagag ggttacggaa tacggaccat tgtggacgcc gaaactgccg
     3541 tcgtaaagca cgaggtaaag gttgaagcgg gccttcttga tattagccgg gattagcgcc
     3601 tgcccagcgg cattaggccc cgagccgccc ggagacaacg cgcctggcgt cgtgtattcc
     3661 cacgcccgtt tcccgtactt ggccgtcaac gagggcggtg ccttagtagt ggcccattga
     3721 tccagagacg ctttcacttc gtttatctga ctcgtgatca cac
//
LOCUS       DATCGS010000404         7029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_628248, whole genome shotgun sequence.
ACCESSION   DATCGS010000404 DATCGS010000000
VERSION     DATCGS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628248"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VIL39_08245"
     CDS             <1..307
                     /locus_tag="VIL39_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481454.1"
                     /translation="IELRLAEGMPLDQAVVDAGAVRFRPMLLTALAVVVGASVILADP
                     IFQGLAISLMAGEIASLLISRMAVPVLYFMAYNRKPGVAAARTESPESSAAPDAPKG"
     gene            531..875
                     /locus_tag="VIL39_08250"
     CDS             531..875
                     /locus_tag="VIL39_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5481455.1"
                     /translation="MQDTSSKPKGLAAALVAKATELVNYQDGAVVSREIVKKPTGNVT
                     LFAFDEGQGLSEHTAPFDALAQVVEGEAEITISGQPHRVLGGEMILMPAGQPHALKAI
                     KRFKMILTMIRS"
     gene            complement(1121..2911)
                     /gene="carB"
                     /locus_tag="VIL39_08255"
     CDS             complement(1121..2911)
                     /gene="carB"
                     /locus_tag="VIL39_08255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5481456.1"
                     /translation="MNAELLAKAKSLGFSDRQIAHLTGQTEDAVRAERKKIGLVPSYR
                     LVDTCAAEFEAYTPYYYSTYDRGDDEVNPSNRKKVMILGGGPNRIGQGIEFDYCCVHA
                     AFALKEDGFETLMVNSNPETVSTDYDTSDKLYFEPLTLEDVLHIYEREKCWGAIAQFG
                     GQTPLNLALSLQRNGVNIIGTSPQSIEIAEDRKLFSAMLTKLNIPQPPNGIATCEAEA
                     LAVGKKLGYPVLVRPSFVLGGRAMQIVYSDEELRHYMRFAVEASPERPVLVDQYLEDA
                     TEVDVDCIADIGNFYYPNQGTIVIGGILEHIEFAGVHSGDAAMVLPPHTLSQRIINTI
                     REYTHAMARELKVVGLMNVQYAVKGDTVYVLEVNPRASRTVPFVSKAIGVPLAKLAAR
                     VMAGKTLKQLGFTKEVWPQYWAVKESVFPFNRFHGQDILLSPEMRSTGEVMGLDDDLG
                     VAYAKSQMAAGGPLPEGGRAFISVSDSHKGGAAEVGRAFAALGFELVATSGTADVLEK
                     AGLKVRRIFKLAEGRPNAVDLLKNREIQIVINTPSGATPHADGVKIRTTAVYTGTPIM
                     TTLSGAKAAALGIAALRKSGYGVKTLQEYH"
     gene            3040..3702
                     /gene="hisB"
                     /locus_tag="VIL39_08260"
     CDS             3040..3702
                     /gene="hisB"
                     /locus_tag="VIL39_08260"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5481457.1"
                     /translation="MKTRRAELRRATKETCIVVKLNIDGAGKSSIQTGIPFFNHMLTL
                     FARHAVVDLKLRCKGDLEVDAHHAVEDCGIALGQAFLQALGDKKGIRRYGTGFDPRNP
                     LTGEAYVPMDECLARCVIDFSGRPFLVWRGLNEFAYKSVSKAEKRQDMSSAFRFGLAR
                     EFFQGFTNEARCNLHLELLYGDEPHHIAEALFKAFAKAVDAACQRDLRMAGQLPTTKG
                     KL"
     gene            3742..4341
                     /locus_tag="VIL39_08265"
     CDS             3742..4341
                     /locus_tag="VIL39_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481458.1"
                     /translation="MTIKDNSSTDDQTLVRASQRGDMAAFEELVARHRDRIYARAYSM
                     MRNEEEAVDLSQEAWVKGWQRLAQFQGESSFGTWMTRIVINLCLDQLRKHKRHRAESI
                     EAMSEESGGVERQMPVVTVNPTAGLEQGELRQRIDQALGQLSYEHRTSLVLHEFEDME
                     YKEIARTMGCSIGTVMSRLFYARRKLAVLLADLKNRDSV"
     gene            4341..4850
                     /locus_tag="VIL39_08270"
     CDS             4341..4850
                     /locus_tag="VIL39_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481459.1"
                     /translation="MDYNAQLKLQALLDGELPEAEASEVTNWVARDREAAALLEELRN
                     TRGALAGFEAEVRLPESREFFWSKVEREIQRLEAPAPKPVRESYLALFRRFLVPASAV
                     AAVLVAGLMLTRPTGPYGRTAVASIKTALAGSGAFTYRDYSAGTTLVWLSYPADTEVA
                     ENDEMGTIE"
     gene            4854..5321
                     /locus_tag="VIL39_08275"
     CDS             4854..5321
                     /locus_tag="VIL39_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481460.1"
                     /translation="MRAKLISVTGLLGFSTALALFAGGELRVQAADMKFQAFLLWGTD
                     DSKPPEGKTYKPVTAEIKQKLKELPLKWANWFEVNRKDFAVPQGAVSEVAMSEKCQVN
                     VKKLAGSEVEVSLIGKGKEVMKRKQSLPTGEMLVLGGNAPNSTAWLVVLKRIE"
     gene            5406..6173
                     /locus_tag="VIL39_08280"
     CDS             5406..6173
                     /locus_tag="VIL39_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481461.1"
                     /translation="MLIRISLIVAIIAGLAVGGLNFVKVKEKITTLQANLKEQTDGRQ
                     KAETELASTKKDLGKRTAELKQTKAELEVATAAKEKADADLAAQTKRADKLTEDLTKT
                     RQERDTAQQDLAAYKVTGVTPQQIVRMNKEFKALQDSIAVAETENKVLMQKVKKLETE
                     LLVYRDPNFFVPLPSGLKGKVLVADPKWNFVVLNIGEDQGVLERGELLVNRNGLLVAK
                     VVVRSVQKDRSIANVLPGWELGQVMEGDQVIPAHPAS"
     gene            complement(6389..>7029)
                     /locus_tag="VIL39_08285"
     CDS             complement(6389..>7029)
                     /locus_tag="VIL39_08285"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY5481462.1"
                     /translation="TVRRPKGDPRQRRSCHRQMQNTIREAKRALRDQVRAAVTRMEPN
                     EKAAASAQARALLAAQPLWRAAQWVLFFAPLPEELDVWPLLAVALSTAKRVALPRYVA
                     KTKTYEACQILDPRLDLQVGHFGVREPHSRRAPLLSTHLDLILVPGVAFDLHGHRLGR
                     GKGYYDQLLKGLSGTTCGVAFDQQIVGEIPIEPHDVRLDCVLTPTRWIQLKP"
BASE COUNT         1380 a         2003 c         2270 g         1376 t
ORIGIN      
        1 catcgaacta cgcctggccg aaggcatgcc gctcgatcaa gcggtggtgg atgccggcgc
       61 ggtgcgcttt cgcccgatgc tgctgacggc tttggccgtg gtggtaggcg ccagcgtgat
      121 cctggccgac ccgatctttc agggcctggc catctcgctg atggcgggcg agattgcgtc
      181 cttgctcatc agccgcatgg ccgtgcccgt gctctacttc atggcctata atcgcaaacc
      241 cggagttgct gccgcccgaa ccgaatcccc tgagagttcc gctgccccgg acgccccgaa
      301 gggctaatag ttcagatttg agggtggaac aaggaaatca ctgccatgag aactgtgtct
      361 cggttcacct tgggcagcct gttctgcggt ctcgcgcttc tcccgccacg tgctcaacgg
      421 ccagttagaa acctccggtc ataatgcctc gccgaccgcg caaaacgttg ggaacttgac
      481 ataaatcaag ccgcgcgggc gcacgcgtgc tactttgatt aagcaaacct atgcaagaca
      541 catcatccaa acccaaggga ctggccgctg ccctggtcgc gaaggctacg gaactcgtca
      601 actaccagga cggcgcggtc gtcagccgcg agatcgtcaa gaaacccacc ggcaacgtga
      661 cgctcttcgc cttcgacgaa ggccagggcc tgagcgagca caccgcccca ttcgatgcgc
      721 tggcgcaagt ggtggaaggc gaggcggaga ttaccatctc cggccaaccg catcgcgtgt
      781 tgggcggcga aatgattctc atgcctgccg gccaacccca cgcgctcaag gccattaagc
      841 ggttcaagat gatcttgacg atgatccgct cgtgacagaa ctccttctgg cctaaccgac
      901 cgggaaacgc gtggggcgac atcggggacg gttcaggtaa cggtctgtcc gctcaccggc
      961 gagcctttgc aagatgtgaa aggcgaggtc gagaagtacg aggtcatcgg cgacatttgc
     1021 tcagccggcg gcaggcgctc ggcctggaac tggcgcggca ggcgctcgaa cccacccgtc
     1081 cggggcgctt cctggctcgg tcaacgggcc gttccatctc ttagtggtat tcctggaggg
     1141 ttttaacacc gtagccgctc ttcctgagcg ccgcgatgcc cagtgccgcc gctttggcgc
     1201 cgctgagcgt ggtcatgatc ggcgtgccgg tataaaccgc ggtggtgcgg attttcacgc
     1261 catccgcatg gggtgtggcg ccggagggtg tgttgatgac gatctggatc tcgcggttct
     1321 tcagcaggtc caccgcgttg ggccggccct cggcgagctt gaagatgcgg cgcactttga
     1381 gcccggcctt ttccagcacg tccgcggtgc cgctcgtcgc caccagttcg aagcccagcg
     1441 cggcgaaggc tctgcccacc tccgcagctc cgcctttgtg cgagtcgctg acactgatga
     1501 acgcgcggcc accctcgggc aacgggccgc ccgcggccat ctgtgatttg gcataggcga
     1561 cgcccaggtc atcgtcgagg cccatgactt cgcccgtgga acgcatctcg ggcgagagga
     1621 ggatgtcctg gccgtggaag cgattgaagg ggaacacgga ttctttgaca gcccaatact
     1681 gcggccagac ctccttggtg aatccaagct gcttgagcgt cttgcccgcc atcacccggg
     1741 cggccagttt cgccagcggc acgccgatgg ctttgctaac gaacggcaca gtgcgcgagg
     1801 cgcgcgggtt gacttccagc acatagacgg tgtcgccttt tacggcgtac tgcacattca
     1861 tcaagccgac caccttcagc tctcgggcca tggcgtgggt gtattcgcgg atggtgttga
     1921 tgatccgctg cgagagcgtg tggggcggca ggaccatcgc cgcatccccg gagtggacgc
     1981 cggcgaactc gatgtgctcg aggatgccgc cgatgacgat ggtgccctgg ttcgggtagt
     2041 agaaattgcc gatgtcggcg atgcaatcca cgtccacctc ggtggcgtcc tcgaggtact
     2101 ggtccaccag cacggggcgt tcaggcgagg cctccacggc gaagcgcatg taatgccgaa
     2161 gctcttcgtc ggaataaacg atctgcatgg cgcgtccgcc gagcacgaag ctcgggcgca
     2221 cgaggaccgg gtagccgagt ttcttcccca cggcgagcgc ctcggcctcg caggtggcga
     2281 tgccgttggg cggctgggga atattgagct tggtcagcat cgccgagaag agctttcggt
     2341 cttcggcaat ctcgatgctt tggggtgaag tgccgatgat gttgaccccg ttcctttgca
     2401 aggacagggc gaggttgagc ggcgtctggc cgccgaactg ggcgatggcg ccccagcact
     2461 tctcgcgctc gtagatatgc agcacgtcct caagtgtgag cggctcgaag tagagtttgt
     2521 cgctggtgtc gtaatccgtg gagacggttt ccgggttcga attgaccatg agggtctcga
     2581 agccgtcctc cttgagcgcg aaggcggcgt gcacgcagca atagtcaaac tcgatgccct
     2641 ggccgatgcg gttcggcccg ccgccgagga tcatcacttt cttgcggttg ctggggttaa
     2701 cctcgtcgtc gccgcggtcg taggtcgagt aataataggg cgtgtaagcc tcgaactcgg
     2761 cggcgcaggt atcaaccagg cggtagctgg gcaccagtcc aatcttcttc cgctcggcgc
     2821 ggacggcgtc ttcggtctga ccggtcaaat gggcgatttg ccggtcggag aatccgagcg
     2881 acttggcttt ggctaacagt tcggcgttca tgcaatccag cgcggataga atcggacaaa
     2941 ggaaaccgta tcaggggtca aatgtcgagt ggcgagtggc gcgaggtcgg aagctttggg
     3001 ctttgtgttt ggctggcgga tttggttaaa tgacccgaaa tgaagacccg acgggcagaa
     3061 ctgcgccgcg cgaccaaaga aacttgtatc gtggtgaagt tgaacattga cggggcgggc
     3121 aagtcgtcca tccagaccgg catccctttc tttaaccaca tgctgacgct gttcgcccgc
     3181 cacgcggtcg tggatctgaa gctcaggtgc aagggggacc tggaggtgga tgcgcaccac
     3241 gcggtggagg actgcggtat cgccctgggg caggctttcc tccaggcgct gggggataag
     3301 aaggggattc gccgctacgg gacgggcttc gacccccgca acccccttac gggcgaggct
     3361 tatgtgccga tggatgaatg cctggcacgg tgcgtgattg acttcagcgg gcggccgttc
     3421 ctggtgtggc gggggttgaa tgagttcgcc tacaaaagcg tcagtaaagc tgagaaaaga
     3481 caggacatgt cgagcgcatt ccgattcggc ctggcccgtg agtttttcca gggcttcacc
     3541 aatgaagccc ggtgcaacct gcatttggag ctgctttacg gggacgaacc acaccatatc
     3601 gcggaggccc tgttcaaggc gtttgccaag gccgtggatg ccgcctgtca acgggacctg
     3661 cggatggccg gccagcttcc cacgaccaaa ggaaaactgt agcccgggtt gaatactcat
     3721 agtttccccg ccgtcgaacc gatgactatt aaagacaatt cctccactga tgatcagaca
     3781 ttggtgcgag cgtcgcagcg cggtgacatg gctgcgttcg aggagttggt cgcgcggcat
     3841 cgcgatagga tttacgcccg cgcttacagc atgatgcgga atgaagaaga agcggttgat
     3901 ctttctcaag aggcatgggt gaagggttgg cagcggttgg cccagttcca gggcgaatcg
     3961 agcttcggca cctggatgac gcggattgtt atcaatctgt gcctggacca gttgcgcaag
     4021 cacaagcggc atcgggctga gtccatcgag gcgatgagcg aagagtcggg cggggttgag
     4081 cggcaaatgc cggtcgtcac ggtgaacccg accgccggct tggagcaggg cgaacttcgc
     4141 cagcggattg accaggcgct gggccagttg tcgtatgagc atcggacgtc gctggtgctg
     4201 catgaatttg aagatatgga atataaagag atcgccagaa cgatgggctg ctccatcggc
     4261 acggtcatgt cgcgtctatt ctacgcgcgc cgaaagctgg cggtgctgct ggcggatttg
     4321 aagaaccgag attcagtgtg atggactaca atgcacaatt gaaactgcaa gccttgctcg
     4381 acggcgagtt gccggaggcc gaggcgagcg aggtcaccaa ctgggtggcc cgggaccgcg
     4441 aagccgcggc cctgctggag gaattgcgca atacccgcgg tgccctcgcg ggttttgagg
     4501 cggaagtccg gctgccggag tcgcgcgagt tcttctggtc caaggtcgag cgggagatcc
     4561 agcgtctgga ggctccggcg cccaaacccg tccgggagtc ttacttggcg ctgttccgcc
     4621 gtttcctggt ccccgcgtct gccgtcgcgg cggtcttggt tgccggattg atgctcaccc
     4681 ggccgacggg cccctatggc cgcacggcgg tagcctcgat caagaccgcg ctggctggct
     4741 cgggcgcttt tacctaccgc gattattcgg ccggcacgac tttggtctgg ctttcgtacc
     4801 ccgccgatac agaagttgcg gaaaacgatg agatgggtac aatcgaatag tcaatgagag
     4861 cgaagctgat atcggtcacc ggcctccttg gtttttcaac cgcgctggct ctgttcgccg
     4921 gcggcgagct gcgcgtccag gcggctgata tgaagttcca ggcgtttttg ctttggggga
     4981 ccgacgacag caaaccgccc gaaggcaaga cctacaagcc ggtcactgct gagatcaagc
     5041 agaagctcaa ggagctcccg ctcaagtggg ctaattggtt tgaggtcaac cggaaggact
     5101 tcgccgtgcc gcagggtgcg gtcagcgaag tcgccatgag cgagaagtgc caggtgaatg
     5161 tgaagaagct cgctggttcc gaggtggaag tctccctgat tggcaagggc aaagaggtga
     5221 tgaaacgaaa gcagtccctg ccgacaggcg agatgctggt cctgggcggc aatgccccaa
     5281 actccaccgc ctggctggtc gtcttgaagc ggattgagtg accgcgcatc gggctgattc
     5341 gggcgttgac accccgggcg ccaacgctta agtttaaccc cgatacttaa atcgaacacc
     5401 gtaccatgtt gattcgaatc agcttaatcg tggccatcat cgccgggctg gcggtcgggg
     5461 gcctgaattt tgtcaaggtt aaggagaaga tcaccaccct ccaagccaac ctcaaagagc
     5521 agaccgacgg acggcagaaa gctgaaacag aattggccag caccaagaag gatttgggca
     5581 agagaaccgc agagctaaag cagaccaagg ctgagttgga agtagccacg gcggcaaagg
     5641 agaaggcaga tgcggatctg gccgcgcaaa ccaagcgtgc tgacaagctg acggaggatc
     5701 tgaccaagac ccgtcaggag cgggacactg cccaacagga tttggcagcc tacaaggtga
     5761 ccggtgtcac tccgcagcag atagtgcgca tgaacaagga attcaaggcg ctgcaggaca
     5821 gtatcgcagt cgcggaaacg gaaaacaagg tgctgatgca gaaggtcaag aagcttgaaa
     5881 ccgagctgct cgtttaccga gatccaaatt tcttcgtgcc gttgccctcc ggcttgaaag
     5941 gcaaggtgct ggtggctgat ccgaagtgga acttcgtggt gctcaatatc ggcgaagacc
     6001 agggggtgtt ggaacgcggc gagttgctgg tgaatcgcaa tggcttgctg gttgccaagg
     6061 ttgtggttcg cagcgtgcaa aaggatcgca gcatcgccaa cgtcctgccg ggttgggaac
     6121 ttggtcaggt catggaaggg gaccaggtca ttcccgccca tcctgcatcg tgagaagtat
     6181 tgcttccatt acgcgctggg cgtttctgct gctgctgctc ttgggtgggt ttgctttggg
     6241 ggggtgcgct actactgagt cggacaacct gtccgagcgc ccttggaacg cgccgcaagg
     6301 ttgggaaaac ggcataccca gcagcatgta taacgaacgc aggtaaggcg acagcttccg
     6361 cttaccgccc ccgaacggtt tctcgcgctt acggtttcaa ctggatccaa cgcgtcggcg
     6421 taaggacgca gtctaagcgc acgtcatgtg gttcaatggg gatttcgccc acgatctgct
     6481 ggtcgaaggc cactccgcag gttgtcccgc tcaatccctt cagcaactgg tcgtagtatc
     6541 ccttgccccg gcccaggcgg tgaccgtgca aatcaaaggc caccccgggg accaggatta
     6601 ggtccagatg ggttgagagg agcggggcgc ggcggctgtg cggctctcga acgccaaagt
     6661 gccccacctg taaatccagc ctgggatcaa gaatttggca ggcctcgtag gtcttggtct
     6721 tcgccacata gcgaggcagg gccacccttt tggcggtgga taaggccacg gccaacagcg
     6781 gccagacatc cagttcttcc ggcagcggcg caaagaacaa gacccactgc gccgctctcc
     6841 atagtggttg cgccgccaac aaagcgcgcg cctgggctga agcggcggcc ttttcgttcg
     6901 gctccatccg cgtgaccgcg gcgcgaacct ggtcgcgcaa cgcgcgcttg gcttctcgaa
     6961 tcgtgttctg catttgccgg tggcaagaac gccgttgcct cggatcaccc ttcggacggc
     7021 ggacggtgg
//
LOCUS       DATCGS010000405         3409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_727470, whole genome shotgun sequence.
ACCESSION   DATCGS010000405 DATCGS010000000
VERSION     DATCGS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727470"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1038)
                     /locus_tag="VIL39_08290"
     CDS             complement(31..1038)
                     /locus_tag="VIL39_08290"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY5481463.1"
                     /translation="MDPELNRRIQDLIDYRGGGFNQESVADIIENALKLMKDVEDSGD
                     VRVIQTALRKLRYAFKLFAPYASVRKVTMFGSARTAPTRPEYQQAVDFGRKIVEAGFM
                     VITGAGPGIMQAGHEGAGPEKSFGANIRLPWEQSANPIIREDKKLVTFKYFFTRKLIF
                     IRHSDALTLFPGGFGTMDEGYEALTLMQTGKSQLMPLVLIDRPGGTFWKTWDKQVREH
                     LLRDQLISPEDLDLYQITDDTDQAVKIITRFYRNFHSTRFVKDLFVIRLKHAPSDSAL
                     AAMNEDFADINTGAPIKRIEPTPEEVEDNEHVDLARIGFIFNRKDYGRLRQMIDVLNS
                     L"
     gene            1183..>3409
                     /gene="ileS"
                     /locus_tag="VIL39_08295"
     CDS             1183..>3409
                     /gene="ileS"
                     /locus_tag="VIL39_08295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY5481464.1"
                     /translation="MDYKNTLNLPHTDFPMKADLITREPARLKKWETAGLYAAIQAQR
                     ASAEKFVLHDGPPFANGDVHIGTALNKILKDIIIKYKTLRGFSAPYVPGWDCHGLPIE
                     FKVTQDMRKAGDTASDAATIRTACDAYARKYIGIQRAQFKRLGVLGDWDNPYLTLAKE
                     YEADELRLFADIVEQGFVYRGKKPVYWSIPCRTALAEAEVEYNDHVSQSVYVKFPLVG
                     HPGTSVVIWTTTPWTLPANLAVAYNSTFSYSVVLVGNEQFIVSALLLPVVAAKCGWEN
                     YQIVRSLDGGHLGQLEYQHPFCQRTGKFLAGDSFVDNVTGTGFVHIAPGHGLDDYHLG
                     ADHGLPIYSPVDDDGKLAHTSDLPKEQQMPVEMLGKSILEKHGQSEANEVVLHELRAR
                     KALLHQENYHHSYPHCWRSKTPIIFRAMDQWFIEIDHAKFRERALDEINCVNWVPDWG
                     KSRIEAAVKGRPDWCISRQRTWGVPIPAFYDAQGNPILDAQIVRNAAALVEKHGSNVW
                     FEKSAPELWAALKPADWKGPEAVTKSNDTLDVWIDSGSSSRSVLARRPELHHASRITH
                     HASPSSSTLAWQADMYLEGSDQHRGWFQSSLLLSLAGNGAAPYKTVLTHGFMVDADRE
                     KISKSKQGQGGYEKPQTSEAYVKKYGADIVRLWVSSQDFRSDIIVSEERISKVAETYR
                     VLRNALRYQLSNLYDFDPAKHTVPDDQLTGLDRWILGEFSQLEQEVIAAYDKYEFHVV
                     YQ"
BASE COUNT          666 a         1073 c         1027 g          643 t
ORIGIN      
        1 cggaacctgg accgcaagtg acatcccgca tcacaggctg ttcagcacat caatcatctg
       61 gcgcagccgg ccgtaatcct tgcggttgaa gatgaagccg atgcgcgcca ggtccacatg
      121 ttcgttgtct tccacctctt cgggcgttgg ctcgatgcgc ttgatgggag cccccgtgtt
      181 gatgtccgcg aagtcctcgt tcatcgctgc gagcgccgaa tcgctggggg catgcttgag
      241 ccggatcacg aacagatcct tcacgaagcg ggtggagtgg aagttgcggt agaaccgtgt
      301 gatgatcttt accgcctggt cggtgtcatc ggtgatctgg tagagatcga gatcctcggg
      361 cgagatcaac tggtcgcgca gcaaatgctc gcgcacctgc ttatcccagg tcttccagaa
      421 agtgccgccg ggccggtcaa tgagcaccag cggcattagc tggctcttgc ccgtctgcat
      481 cagcgtcagc gcctcatagc cttcgtccat ggtgccaaac ccgccgggga atagcgtgag
      541 ggcgtccgag tgccggatga agatcagctt gcgggtgaag aaatacttga aggtgaccag
      601 cttcttgtcc tcgcgaatga tcgggttggc ggattgttcc cagggcaacc ggatgttggc
      661 gccgaaactc ttctccgggc cggcgccctc gtgtccggcc tgcatgatgc ccgggcccgc
      721 gccggtaatg accatgaagc ccgcctccac gatcttgcgg ccgaaatcca ccgcctgttg
      781 gtattccggc cgggtcggcg ccgtccgggc ggagccgaac atggtgacct tgcgcacgct
      841 tgcataaggg gcaaagagct tgaaggcata ccgcagcttg cgcagcgccg tctgaatgac
      901 gcgcacgtcg ccgctgtcct ccacgtcctt catcagcttc agcgcgttct cgataatgtc
      961 cgccacgctt tcctggttga agcctccgcc tctatagtca atcaggtcct ggatccgccg
     1021 gttcagttca gggtccatcg ggggccgttc tggtctaatc atggaaacga tgataagcgg
     1081 ctgggagccg aaagcaaccg cctgcgcagc gccgggcggt tcgtgcggtt cgttgacact
     1141 tctccgcatg gctgatagct tgcgctgtct ttgagacgct aaatggacta caagaacaca
     1201 ttaaatctgc cgcacaccga tttcccgatg aaggcggacc tgatcacgcg ggaaccggcc
     1261 cggctgaaga agtgggagac cgccgggctc tacgccgcca tccaggccca gcgcgccagc
     1321 gcggagaagt ttgtcctgca cgacggaccg cccttcgcca atggcgatgt gcatatcggc
     1381 accgccttga acaagattct caaggacatc atcatcaagt ataagacgct gcgcggcttc
     1441 agcgcgccgt acgtgccggg ctgggattgc cacggcctgc cgatcgagtt caaggtcacg
     1501 caggacatgc gcaaggcggg cgataccgcc tccgatgccg cgaccatccg cacggcgtgc
     1561 gacgcctacg cgcgcaaata catcggcatc cagcgcgcgc agttcaagcg ccttggcgtc
     1621 ctcggggatt gggataatcc ctacctgacg ctggccaagg aatacgaggc ggacgagctg
     1681 cggctgttcg cggacatcgt ggagcaggga tttgtctatc gcggcaagaa gccggtgtat
     1741 tggagcatcc cctgccgcac cgcgctggcg gaggccgaag tcgaatacaa tgaccacgtc
     1801 agccagagcg tgtatgtgaa gtttcccctg gtcggacatc ccggcacttc cgtcgtgatc
     1861 tggactacga cgccctggac gctgccggcg aaccttgcgg tggcttacaa ctccacgttt
     1921 agctactccg tggtgctagt cggcaacgag caattcatcg tctccgcgct cctgctgccg
     1981 gtggtggccg caaagtgcgg ctgggagaac taccagatcg tccgcagcct cgatggcggg
     2041 catctgggcc agcttgaata ccagcatccc ttctgccagc gcaccgggaa gttcttagcc
     2101 ggcgacagct tcgtggacaa tgtcaccggc accggcttcg tgcatatcgc gccggggcac
     2161 ggcttggatg actaccacct cggcgccgat catggcttgc ccatttactc gccggtggac
     2221 gatgacggaa agcttgctca caccagtgac ctgccgaagg agcagcagat gccggtcgag
     2281 atgctcggca agtccattct ggagaagcac ggccagagcg aagcgaacga agtcgtgttg
     2341 cacgaactgc gcgcccgcaa ggcgctcctg catcaggaga actatcatca cagttatccg
     2401 cactgctggc gcagcaagac gcccatcatc tttcgcgcca tggatcagtg gttcattgag
     2461 attgaccacg cgaagttccg cgagcgggcg ctcgacgaga ttaactgcgt gaactgggtg
     2521 cctgactggg gcaagagccg catcgaggcc gccgtgaaag gccggcccga ctggtgcatc
     2581 tcgcgccagc gcacgtgggg cgtccccatc cccgcgttct acgacgcgca aggtaatccc
     2641 atcctggacg cgcaaatcgt gcgcaacgcc gccgccctgg tggagaagca cggctcgaat
     2701 gtctggttcg agaagtctgc ccctgagctg tgggccgcgc tcaaaccggc cgattggaaa
     2761 gggcctgagg cggtcaccaa atccaacgac acgctcgacg tgtggattga ttcgggctcc
     2821 tcctcacgct cggtcctggc gcgtcgtcct gagcttcatc acgcatcacg catcacgcat
     2881 cacgcatccc catcctcctc gaccctcgcc tggcaagccg acatgtacct cgaaggcagc
     2941 gaccagcatc gcggctggtt ccagtcgtcg ctgctcctct cgctggcggg caacggcgcg
     3001 gcaccctaca agaccgtgct gacccacggc ttcatggtgg atgccgaccg cgagaagatc
     3061 tccaagagca agcagggtca gggcggctac gagaagccgc agacctccga agcctacgtt
     3121 aagaaatacg gcgccgacat cgtgcgcctc tgggtttcct cacaggactt ccgcagcgac
     3181 atcatcgtca gcgaggagcg gatcagcaag gtggccgaga cctaccgcgt cctccgcaac
     3241 gcgctgcgct accagctctc gaacctctac gacttcgacc cagccaaaca cacggtgccc
     3301 gacgaccagt tgaccgggtt ggatcgctgg atactgggtg agttctcgca gttggagcaa
     3361 gaagtcatcg cggcctacga caaatacgag ttccacgtcg tttaccaga
//
LOCUS       DATCGS010000406         5902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_84382, whole genome shotgun sequence.
ACCESSION   DATCGS010000406 DATCGS010000000
VERSION     DATCGS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84382"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VIL39_08300"
     CDS             <1..878
                     /locus_tag="VIL39_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HEY5481465.1"
                     /translation="NAPGKSAGDVEMLVLDGPAAEDAQFNLEPRRNAASVHLMYPVPK
                     GTNVEAFYCEVIAVEDPLWTYYMACGWHRGYFGMQVNSPTERRIIFSVWDSGNEGVDR
                     GQVAAENRVMLVAKGEGVYTGDFGNEGTGGHSHLVYPWKTGEKQRFLVTARPTDDTHT
                     VCSGYYFHPDKKQWVLISAWKASKEGGYLRGLHSFSENFGGANGHLRRKALYGNQWIR
                     SADGQWIELTTASFSHDTTGRADRLDRFMGVENGQFFLSHGGFVPGSTPFGVKFIRPA
                     TGKAPSDIVLRLLPQ"
     gene            903..2087
                     /locus_tag="VIL39_08305"
     CDS             903..2087
                     /locus_tag="VIL39_08305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY5481466.1"
                     /translation="MYDSFKPHLEAQLASIRAAGTYKHERVIMTPQGTTVRVADGQPV
                     LNLCANNYLGLAQHPAVAAAAKEAIDKWGYGLASVRFICGTQGVHKQLERKLSEFLGT
                     DDTILYGSCFDANGGLFETLLGPEDVIISDELNHASIIDGVRLCKAQRLRYLNSNMAD
                     LEAKLKEASSARFKMIATDGVFSMDGYIANLPGICDLAEKYGALVMVDDSHAVGFMGK
                     HGRGTHEHCGVMGRVDIITGTLGKALGGASGGYTSGRKEIIELLRQRSRPYLFSNTLA
                     PGIAGASLKVLELLSESTALRDQLEANTKFFRDGAAKLGFTILPGTHPICPVMLGDAG
                     LAARFADAMLEKGVYVIGFSYPVVPNGKARIRTQISAAHSLEELALALETFAETKREL
                     GI"
     gene            2769..3392
                     /locus_tag="VIL39_08310"
     CDS             2769..3392
                     /locus_tag="VIL39_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5481467.1"
                     /translation="MTKSVIVVEDDRGLREQLVKILNSALGIRCVGACISGEDAVRLV
                     PTKKPDVVLMDIRLPGMSGIDCVSELKQILPGLHIIMLTVYEDSESIFRALKAGANGY
                     LVKSSPPGKLLEAIQDVSKGGAPMSSPIARKVVQHFHLLGSPRTRAEDLSPREREVLE
                     LLASGYIYKEIGDKLGIGVETVRTYVKNICEKMHVRSRLEAVAKHRS"
     gene            complement(3429..5129)
                     /locus_tag="VIL39_08315"
     CDS             complement(3429..5129)
                     /locus_tag="VIL39_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5481468.1"
                     /translation="MSASFRITGLSLGCLLGGLSWCLALEGLPGGKTDQLLEIRSLSV
                     NGQPVSLRPNSRLRLSPTPRNILFGFGPATNSEHAPLRVRYKLDGFEDNWREASSDLS
                     ISFRFIDTNQDPVSEKAFRAVGQTEEWTGALDTSAFVHRRETILVPPGAKGLWVSVSS
                     AGPPNTVGMYAVTNLVVTRLPANNQPSAVLLRWDSDAKGEFAGSEWVPADWTRNGLRV
                     SMAKITQFGPDQKLKALTLLDNDPKAHAEWTTRKEAAPAVTPGDQLVLEWDEAFSLGL
                     AGPAEVTYAELPAGFYRFRVGELSLLGLPGEAETSLAFEVPLSFWRTPWFWGLVFLLA
                     LFGAVGTYRYIGWQQMRRKLAHLESQRALEHERVRIARDIHDDLGARVTQISLVSGFA
                     QGDPTLSEKARGDFNAISGMARELVSALYETVWAVNPENDNLDALGNYICQMVDNLCD
                     KAQLPHRLRVAELPRDVQVSSRVRHDLIMAVKEAVHNVIKHAKASELSVYVDWEGTTL
                     TIRVQDNGCGMDPATRGAGSGLTNMQRRLEHTGGTCSFHSEPGQGTTVVFRSDIQSPG
                     "
BASE COUNT         1187 a         1652 c         1928 g         1135 t
ORIGIN      
        1 tgaatgcgcc cggcaaatcg gccggggatg tcgaaatgct cgttctcgat ggccccgccg
       61 cagaagacgc ccagttcaat cttgagcccc ggcgcaacgc ggcttcggta cacctcatgt
      121 atccggtgcc gaaggggaca aacgttgagg cgttctattg cgaggtgata gccgtggaag
      181 acccgctctg gacttattac atggcctgcg gctggcaccg cgggtatttc ggcatgcaag
      241 tcaacagccc caccgaacgt cgcataatct tcagcgtctg ggacagcggg aacgagggtg
      301 tggaccgtgg gcaagtcgct gcggaaaacc gggttatgct tgtggccaaa ggcgagggcg
      361 tttacaccgg cgacttcggc aatgaaggca ctggcggcca cagccatttg gtctatccct
      421 ggaagacagg cgaaaaacag cggtttctcg tcactgcccg gcccaccgac gacacgcaca
      481 ccgtctgctc cggctactat ttccacccgg acaagaagca atgggtgctc atctccgcct
      541 ggaaagcgtc aaaggagggc gggtacctgc gcggtctgca tagcttcagc gagaatttcg
      601 gcggggccaa cggccacttg cgccgcaaag ccctttacgg caaccaatgg attcgcagcg
      661 cggacggcca gtggatcgaa ctcaccactg ccagcttcag ccacgatacg accggcaggg
      721 ccgatcgtct ggaccgcttc atgggcgtgg agaacggtca gttctttctt tctcacggcg
      781 gttttgtccc aggctccaca ccgttcgggg tgaaattcat ccgcccggcc accggcaagg
      841 cgccctcgga cattgtgctg cggctgttgc ctcaatgaga gtgattctca accgaaaaac
      901 ctatgtacga ctcattcaaa ccccatcttg aagcccaatt ggcaagcatt cgcgccgccg
      961 gcacttacaa acacgagcgc gtcatcatga ccccgcaggg gaccacagtt cgcgtggccg
     1021 acggtcagcc ggtgctgaac ttatgcgcga ataattacct cggccttgcg cagcatcccg
     1081 ctgtggcggc ggcggcgaaa gaggccatcg acaagtgggg ttacggcctg gcgagcgtcc
     1141 ggttcatttg tggcacacaa ggtgtccaca agcagcttga acggaaactc agcgagtttc
     1201 tgggcaccga tgacaccatc ctttatggct cgtgcttcga tgcgaacggg ggactcttcg
     1261 aaacgctgct tgggccggaa gacgtgatca tcagcgacga gttgaaccac gcgagcatca
     1321 ttgatggcgt gcgcctctgc aaagcgcagc gtctccgcta tctgaacagc aacatggccg
     1381 acctcgaagc gaagctcaag gaagccagca gcgcgcgctt caagatgatc gccaccgacg
     1441 gcgtgttttc gatggacggc tatatcgcga acctgccggg catttgcgat ctcgccgaga
     1501 agtacggcgc gctggtgatg gtggatgatt cgcacgcggt tggtttcatg ggcaaacacg
     1561 gtcgtggcac gcatgagcac tgcggcgtga tgggccgggt ggatatcatc accggcacgc
     1621 tgggaaaggc cctgggcggg gcgagcggcg gttacaccag cggtcgcaag gaaatcatcg
     1681 aactgctccg ccagcgctca cgaccatacc tattttcaaa cacgctcgcg ccgggcatcg
     1741 ccggggcgtc gctgaaggtg ctggagcttt tgagcgaatc caccgcgctg cgtgaccaac
     1801 tggaggcgaa cacgaagttc ttccgcgatg gcgcggcgaa gctcggcttc acgattctgc
     1861 ccggcacgca tccgatctgc ccggtgatgc ttggtgacgc cgggctggcg gcgcgatttg
     1921 cggacgcgat gcttgagaaa ggcgtttatg tcattggctt cagctatccg gtcgttccga
     1981 atggcaaagc gcgcatccgc acgcagattt ccgcagccca cagcctcgaa gagctggccc
     2041 tggccctcga aacgttcgct gaaacaaagc gagaattggg gatctgactc gaccagggca
     2101 agattcttct cccactgccg actattcgac caagtgttga cgccgtctca ggcagtctcc
     2161 cggagctggt gatgatgcag acctttgccc tgcagtctgg gcttgtgcgc cagtcggttg
     2221 agcaccgcga tctggtgccg gagggccaaa ttctccaagg ccagttcgtg tttcggacga
     2281 aggcaggaca acagttaccg cagaagcgcc gagacaatcg cgaacatggc tgattagctc
     2341 tggttccacc atggctggat acgagcagcc cggatcggtg ctctccgctg tggtcggcga
     2401 ggtccacctt tgccgtgtac agggttaaca atctcagcta gcgggcaagt gcctgcggtt
     2461 gttggtccac ggccgccgtt tccgtgagga ccgttcaatt ccaccttgca cctttcgcgg
     2521 gacagtttct tcgcttcggc catgaaatca agagcaggcg tttccgggaa aactgccctc
     2581 cccgctttcg gggatgccgg aggaatggga atgagagtag ggtgcggagg atttgatgag
     2641 cagactatgc agacgcagtt gataagtcag cggttatgaa cccgattccc gatccttctg
     2701 gcgcctccgg gcctagtcca gttaccacta gcgggggtcc ggcggctgga ttagcctcga
     2761 ccggtgttat gactaagtcc gtgatcgtgg ttgaagatga ccgagggttg cgcgagcaac
     2821 tcgtcaagat cctgaattcc gccttgggga tacggtgtgt gggggcgtgc atatcgggtg
     2881 aagacgcggt gcggctggtt ccgactaaga agcccgacgt ggtgctgatg gatatccggt
     2941 tgccggggat gtcgggcatc gattgcgtat cggagttgaa acagattcta cccgggctgc
     3001 acatcatcat gctgacagtg tacgaggaca gcgaaagcat ttttcgggcg ttgaaagcgg
     3061 gcgcgaatgg ctacctggtg aagtcgagtc cgccggggaa gctgctcgaa gcgattcaag
     3121 atgtgtctaa ggggggcgcg ccaatgtcga gcccgattgc gcggaaggtg gtgcagcact
     3181 ttcacttatt ggggtctccg aggactcggg cggaagattt atcgccccgc gaacgagagg
     3241 tgctggagtt gctggcgtcc ggatatattt acaaggaaat cggggacaag ctgggaatcg
     3301 gggtggagac ggtgcggacg tacgtcaaga atatctgcga gaagatgcac gtgcggagcc
     3361 gattagaggc ggtcgccaag catcggtctt agcaggaact tcgcgaacag ggtgaactca
     3421 aggtggagtc accctgggga ctggatgtcg gagcggaaca cgacggtggt tccctgaccg
     3481 ggctcactgt ggaaggagca ggtcccgccc gtgtgctcga ggcggcgctg catgttcgta
     3541 aggccgctgc cggcgccgcg ggtggcgggg tccatgccgc acccattatc ctgaacgcga
     3601 atggtgaggg tggtcccttc ccaatctacg taaaccgaga gttcggaagc cttggcgtgt
     3661 ttgatcacat tatgcacggc ttctttgacg gccataatga gatcgtgccg gacgcggctg
     3721 gaaacctgaa cgtcgcgcgg caactcggcg acgcgcaggc ggtggggaag ctgggccttg
     3781 tcgcagaggt tatcaaccat ttgacagata taattgccga gggcgtcgag gttgtcgttt
     3841 tcggggttca cggcccaaac ggtttcataa agggcggaga caagttcgcg ggccatgccg
     3901 gagatggcgt tgaagtctcc gcgggctttt tccgaaagcg tggggtctcc ttgcgcaaag
     3961 ccactgacta aactgatctg agtgacgcgg gcgccaaggt cgtcatggat gtcgcgggcg
     4021 atgcggacgc gctcgtgttc gagagcgcgc tggctttcga ggtgagccag cttgcgacgc
     4081 atttgttgcc agccgatata gcggtaagta ccgaccgcgc caaacaaggc gaggaggaat
     4141 accagccccc agaaccatgg tgtccgccag aaggacaacg gcacttcaaa agcgagcgag
     4201 gtctcggcct cgccggggag ccccaggagc gacagctcgc caacgcgaaa gcgatagaag
     4261 ccggcgggca attcggcata ggtgacttcg gcgggcccgg cgagcccgag gctgaaggct
     4321 tcgtcccact cgagcacgag ctgatcgccg ggcgtgacgg ctggggcggc ttctttgcgg
     4381 gtagtccatt cggcgtgcgc tttcggatcg ttatcgagga gggtgagggc cttcaatttt
     4441 tgatccggac cgaactgtgt tattttggcc atgctaacgc ggaggccgtt acgcgtccaa
     4501 tcggcgggga cccactccga accggcgaat tcgcctttgg cgtctgagtc ccagcgcagg
     4561 agaaccgcgg agggctgatt gttggccgga aggcgggtga cgacgaggtt ggtgacggcg
     4621 tacatgccga cggtattcgg cggcccggcc gaggaaacgg agacccatag gcccttggcg
     4681 ccgggaggca cgagaatggt ttcacggcga tgcacaaaag ccgaggtgtc cagcgcgccg
     4741 gtccattctt cggtttgccc cacggcgcgg aaggcttttt cgctgacggg gtcctggttg
     4801 gtgtcgatga accgaaacga gatgctcagg tcactggaag cctcacgcca attgtcctcg
     4861 aagccgtcga gcttgtagcg cacgcgcaat ggggcgtgtt ccgagttagt agccgggccg
     4921 aagccgaaga ggatattccg gggcgtgggg gacagacgca gccggctgtt ggggcgcagg
     4981 gaaacgggct ggccgttgac gctgagcgag cggatttcga gaagctgatc ggtcttgccg
     5041 cccggcagac cctcgagcgc gagacaccaa ctcaacccgc cgaggaggca gccgaggctc
     5101 aaacctgtta tgcggaacga ggcgctcatt cagttggccc tcccgcgcgc aggcgggagg
     5161 gaaacgcatt ctgccctgaa cgagcaccgg gcggaagaga atattgacaa tatcccaatc
     5221 aacacagcgc caattcatga aggtcctttg gcgcaaagga ggtgcttcat tctgggctgc
     5281 tggctgcggg gtgattcggg ctcaggggct agcgccccat gggctgaggg ttctgaaagc
     5341 ggcaagagcc ccgcagggtg gagttccaag cgcgtcagga ggggtcggcc ccctggatgc
     5401 cctccaggcg ctcgacacca cccccggccc ggtgtcgcta gggtgtctcc accgtattgc
     5461 tacggtattg ctacggtatc tccccggtgt tgctcccatg ttgatggcat caacatggga
     5521 gaaacaccgg ggagataccg cagcaatacc ggagcaatac ggtggagaca ccatagcgac
     5581 accgggccgg gggtggtgtc gagcgcctgg gggcatccag ggggccgacc catcctgacg
     5641 cgcttggaac tccaccctgc ggggctcttg ccgctgccag aaccctcagc ccatggggcg
     5701 ctagcccctg agcccgaatc accccgcagc cagcagccca gaaagaagca cctcctttgc
     5761 gccaaagggc cttcatgaat tggcgctgtc atataataag cgtatcgcat ttgcgctgga
     5821 cagcaatttg cctagtgcta atttattagt tgagtggttt gcgcgcggga cgtatttgag
     5881 gcggccgcag accttgaagg tg
//
LOCUS       DATCGS010000407         6346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_134546, whole genome shotgun sequence.
ACCESSION   DATCGS010000407 DATCGS010000000
VERSION     DATCGS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134546"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VIL39_08320"
     CDS             complement(<1..545)
                     /locus_tag="VIL39_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481469.1"
                     /translation="MTRLTAILKRMSAVAAGVACLATLSPLLAFAATPVRVSLVISDD
                     QAKPVQHGLRKLRGSLQEKGAQVEATASIEKATGDAVVVAGLSSGSGEVKKLMAELRL
                     DPSKTPESLLIRKLQREGKTFVLITGADACGLMYGLLDVADRVGWARSGEQLFSEVRN
                     TEEQPFTPERALSLYTFNRAY"
     gene            complement(654..1796)
                     /gene="tgt"
                     /locus_tag="VIL39_08325"
     CDS             complement(654..1796)
                     /gene="tgt"
                     /locus_tag="VIL39_08325"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY5481470.1"
                     /translation="MFELLKTDASSHARLGRLTTPHGVVDTPVFMSVGTQGSVKAVDP
                     RELCEMGTQIILGNTYHLNIRPGLDIIRAAGGLHRFINWERPILTDSGGFQVFSLATI
                     RKIQAHGVEFRSHLDGSLLFLGPKEAMAIQAVLGSDIAMVFDDCPPHTSTPRELRAAV
                     ERTMCWAGECREQPRAPGQRVFGIVQGGANAAMREECAKALVALKFDGYAIGGVSVGE
                     PEPEMMRAVEMTEPFLPANQPRYAMGLGTPAQMVELVARGVDMFDCVLPTRVARNGTA
                     FTRKGSLSIKAGACKADFRPIEEGCDCFACQRFSRAYLRHLLNVGEILGLRMLSVHNT
                     RMYLRTMAEMRAAIAAGTFAEFRREFVANYVPTKKVLLARAAVADR"
     gene            complement(1944..2852)
                     /locus_tag="VIL39_08330"
     CDS             complement(1944..2852)
                     /locus_tag="VIL39_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5481471.1"
                     /translation="MKKALIINAKSRRAARPAWLKFWGVRGSIPTPGPTTVQYGGNTS
                     CVEVRADGQIIILDAGTGLRLLGSALVAEFDTRPLDLTLLLTHTHWDHIQGLPFFLPV
                     YKPQNHLHILGYEGARHGLEAVLAGQMESPFFPIGLREVPANVRIEELKEMNFDVGPV
                     RVQACFANHPGKCFGYRLFTSAGSIAFFPDNELNPQDASESRKLINFLRGTDVLIMDT
                     QFDTEEYQRHTGWGHGCLDDVVALALDAEAKKLFLFHHDPNHDDAKISQMLAHARKLV
                     AARKGKLQVEAAREGATVELAAAVRR"
     gene            complement(3078..3788)
                     /locus_tag="VIL39_08335"
     CDS             complement(3078..3788)
                     /locus_tag="VIL39_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481472.1"
                     /translation="MQTNRQTWQSFEEMSKAAEQGDAAAQCYFGICYQTGHGVAQDYQ
                     QALRWFRLAADQDDAAAQCYLGFCYQSGLGVPQEFGQAAKWFREAAEQGNPAAQFNLG
                     VLYETGQGVPQNYAEAVKWYHAAAEQGEPQAQFNLGVFYETGQVVPQNHEEAVKWYRL
                     SAEQECAPAQCNLGLCYETGRGVPKDVRQAVRWFCRAARAGDKTAQHNLGVYYASLEA
                     AEEATAEDGATAADEATA"
     gene            4055..5992
                     /locus_tag="VIL39_08340"
     CDS             4055..5992
                     /locus_tag="VIL39_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481473.1"
                     /translation="MSELQIIESALQQAGRRRRWARALRGLWWGLLVGAVLSLVVIGA
                     WHLLPLPFWTLTTAALAPILCALGGMLIGGWHKTPLPEMARWVDGRQHLKERLSTALE
                     VAGEPDAGSWHDLIVTDAAEHAKGLDLRRLVPFRLPRATRWALVILALGAGLGFVPEY
                     RSKAFLQKKADAQNIKEVGKRLADLTRRTVEKRAPALEPTQKAMETVTELGDQLTKQA
                     LTRSEALKDLANVAEKLKNELKEMGKDPALRKMEQAARASTGNDSQTAAGLQKQIEAM
                     QKQMGTPTGNPEAIDKLKKDLEKLQEAAKGMANKNTPGSQADRQKMSESLSALSKQMQ
                     DMGLQLPQLDDAIKALAENQTDLVLKDLEASLTDLEKMRDMAKSLQQLQQQATKLGKD
                     LAEQLKNGQPEQAQATLQKMVAQLKLPNLAPEQLQKILAEVSKAVDPAGNYGKVAEHL
                     KNAGQQMQAGNKPGAAQALAAAAKELENLTQQMGDAQALMATLDSLNQASMCIGSGQG
                     WGTHTGNKPGYSPFGKGPGGGVGTWGDPNAQWDGQWSDHWDNSGANRPDQDGMGHTDR
                     GEGELSDALKPTKVKGQFKPGGQMPSITLKGVSIKGQSKVAYEEAATAAQSDAQSALS
                     QEKVPRAYQGAVKDYFDDLKK"
     gene            5997..>6346
                     /locus_tag="VIL39_08345"
     CDS             5997..>6346
                     /locus_tag="VIL39_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5481474.1"
                     /translation="MNTEEQIQTFRQAHSALRAELGKVIVGQDSVVEGTLIALFAGGH
                     VLLEGVPGLGKTLLVRTLGEVLDLSFSRIQFTPDLMPADILGTNIVMEISGGRREFQF
                     QRGPIFAHLVLADE"
BASE COUNT         1216 a         1977 c         2018 g         1135 t
ORIGIN      
        1 caataagcgc ggttgaacgt gtagagggac aacgcccgct ccggggtgaa cggctgttcc
       61 tctgtattgc ggacctcgct gaagagttgt tcccctgacc tcgcccaacc gacgcggtct
      121 gccacgtcca gcaacccgta catcaacccg cacgcgtcgg ctccagtgat cagcacgaag
      181 gtcttgccct cgcgttggag cttccggatg agcagcgact cgggcgtctt ggatgggtcg
      241 agccgcaatt cggccatcag cttctttacc tctccggaac cagacgagag gcccgccacg
      301 accactgcgt cgccggtggc cttctcgatc gaggctgttg cctcgacctg tgcccctttc
      361 tcttggaggg atcccctaag tttgcgtaat ccgtgctgga cgggtttcgc ctggtcgtcg
      421 ctgatgacca gcgaaaccct tacaggggtg gcggcgaagg ccaaaagtgg agagagtgtc
      481 gcaagacagg cgacaccagc agccactgcc gacattcgct tcaagatcgc tgtcagccga
      541 gtcatggctg aatttaacca gagccaatat cttttggcga cgtttttgga ggattacttg
      601 cgtttcgctc aaaccggcca tagtagcgtt ccacgcgatg gaacttgcaa cccttatcga
      661 tccgctacgg cggcgcgggc cagcaggact ttcttcgtcg gcacatagtt ggcgacgaac
      721 tcgcgccgga actcggcaaa cgtccccgcc gcaattgccg cgcgcatctc cgccatggtc
      781 cgcaaataca tccgcgtgtt gtggacgctt aacatgcgca gaccgaggat ttcgcccacg
      841 ttgagcaggt gccgtaaata cgcgcgcgag aaacgctggc aggcgaaaca atcacagcct
      901 tcctcgattg gccggaagtc cgctttgcag gccccggcct tgatgctgag gctgcccttg
      961 cgcgtgaagg ccgtgccgtt gcgcgccacc cgtgtcggca ggacgcaatc gaacatatcc
     1021 acgccgcgcg cgaccagctc caccatctgc gcgggagtgc cgagccccat cgcgtaccgc
     1081 ggctggttcg ccggcaggaa tggctccgtc atctccaccg cccgcatcat ctccggttcc
     1141 ggctcgccca cgctgacgcc gccgatggcg tagccgtcga acttcagcgc caccaaggct
     1201 ttcgcgcact cctcgcgcat cgccgcgttg gcgccgccct ggacgatgcc gaagaccctc
     1261 tggcccggcg cgcgcggttg ttcgcggcat tcccccgccc aacacatcgt ccgctccacc
     1321 gcggcgcgca actcgcgcgg ggtgctggta tgcggcgggc aatcgtcgaa caccatcgcg
     1381 atgtccgagc cgagaacagc ttggatggcc atggcttcct tgggccccag aaagagcagc
     1441 gagccgtcca ggtgcgagcg gaactcgacg ccgtgcgcct ggatcttgcg gatcgtcgcc
     1501 aggctgaaca cctggaagcc gccgctgtcg gtcaggatcg gtcgctccca attgatgaac
     1561 cggtgcagcc cgcccgcggc gcggatgatg tccaggccgg gccggatgtt gaggtgatag
     1621 gtattgccga ggatgatctg ggtgcccatc tcgcacaact cgcgcgggtc caccgccttg
     1681 acgcttccct gcgtgccgac ggacatgaag accggcgtat ccacgacgcc atgcggcgtg
     1741 gtcaaccggc ccaggcgcgc gtgggaactc gcgtcggtct taagcagctc aaacatcgca
     1801 gcgagggttt actaccagcc aggctggttc aactgcaaaa acagcgctag gatcggaacg
     1861 taatctcgtg ctgtcggcat cggcgcgatt cggtccatgt tccttggcgg gcccgcttgg
     1921 cctgccgcgc ccgagcaatg ccgtcaacgc cgcaccgccg cagccagttc caccgtcgcc
     1981 ccttcacggg cagcctcgac ctgaagcttg cctttccggg cggcgacgag cttccgggcg
     2041 tgggccagca tttgggagat tttagcgtcg tcgtggttgg ggtcgtgatg gaacagaaag
     2101 agcttcttcg cttccgcatc gagagctaac gcgaccacat cgtccaggca gccgtggccc
     2161 caaccggtgt gccgttggta ttcttcggta tcgaactgcg tgtccatgat cagcacgtcg
     2221 gtgccgcgca ggaagttgat cagcttgcgg ctttcgctcg catcctgagg attcagttcg
     2281 ttgtcgggga agaaggcgat ggagccggcg gaggtgaaca agcggtagcc gaagcatttc
     2341 ccggggtgat tggcgaagca agcctgcacg cgcaccgggc cgacatcgaa gttcatctct
     2401 ttgagttctt caatgcggac gttggctggc acttcgcgca agccaatcgg gaagaacggg
     2461 ctttccatct ggccggccag cacggcttcc aggccatggc gcgcgccttc gtagccgagg
     2521 atgtgcagat ggttctgcgg cttatagacc gggagaaaaa acggcagccc ctggatgtgg
     2581 tcccagtggg tgtgcgtgag cagcaaagtc aagtcgagcg gccgggtgtc gaattcggcg
     2641 acgagtgcgc tccccagcag gcgcagtccg gtgccggcat cgagaatgat gatctgccca
     2701 tcggcgcgga cctcgacaca ggacgtgttg ccgccgtact ggacggtggt cgggcctggg
     2761 gtcggaatgg agccgcgtac gccccagaac ttcagccacg ccggccgtgc ggcgcgtcgg
     2821 gatttagcat tgatgattaa ggctttcttc atggggccac gtggcgcact ctgttacgca
     2881 tcctaacgca tctcaggcca caattaaagc ttaaatgcat tgaaagcgaa cgctgactcc
     2941 atgcagccga ccgcagctca agatcaaacg gtagtggtga ccggcttgtc gaaccgcgag
     3001 ttcctggaac tctacgcccg gcccttagcg tgggtgcttt ccgcgtcgca agatctgccg
     3061 agaatccagc cgccgggtta agccgtggcc tcgtctgccg ccgtggctcc gtcttccgca
     3121 gtcgcctctt cggccgcttc caagcttgcg tagtaaactc cgagattgtg ttgggcggtc
     3181 ttgtcgccgg cgcgtgcggc gcggcagaac catctgaccg cctggcgcac atccttcggc
     3241 acgccccgac ccgtttcata gcaaaggccg aggttgcact gcgccggggc gcactcctgc
     3301 tccgcggaga gcctatacca tttcaccgcc tcctcgtggt tctgcggcac cacctggccg
     3361 gtctcataga agacccccag gttaaactgc gcctggggtt cgccctgttc cgccgcggca
     3421 tgataccatt tgacggcttc ggcgtaattc tgcggcacgc cctggccggt ttcataaagc
     3481 accccgagat tgaactgcgc cgccggattg ccctgctcgg cggcctcgcg aaaccacttg
     3541 gccgcctgtc caaactcctg cggcaccccc aggccggact ggtagcagaa gcccaggtag
     3601 cactgcgccg ccgcgtcgtc ctggtcggcg gcgaggcgaa accaacggag ggcttgctgg
     3661 tagtcctgcg ccaccccatg cccggtttga tagcaaatgc cgaaatagca ctgcgccgcc
     3721 gcgtcccctt gctcggcggc tttgctcatt tcctcaaagg actgccaggt ctgccggttc
     3781 gtttgcatca attggctggt tactcttccg cgaattgctt cagacctggc agcctcgcgc
     3841 cgcactggtc cttgcgctcg cagccaaaga atagagtcaa acgccccgaa ttcaagccgg
     3901 gatttggacg gctcggctgg ccgggcgtac aaacgccatc ctcctccctc tgcctgccgt
     3961 tttgttcttt tccccccaac tttttggccg cagccgtgtc taatacatta gaactggcca
     4021 aaggtgcgcg ggtgctgcaa gtttggccga ggccatgagc gaactgcaaa tcatcgaatc
     4081 ggcgcttcag caggcgggcc ggcgccgccg ctgggcgcgg gcgttgcgcg gcttgtggtg
     4141 gggtctgttg gtcggggccg tattgtcgct ggtggtgatt ggagcatggc atttgctgcc
     4201 gctgccgttc tggacactga ctacggctgc gctcgctcct atcctgtgcg cgctgggggg
     4261 aatgcttatt ggcggctggc acaagacgcc tttgccggaa atggcgcgct gggtagatgg
     4321 gcgccagcat ctgaaggagc ggctgagcac ggctctggaa gtcgccggcg agccggacgc
     4381 cgggtcgtgg cacgacctca tcgtcaccga tgccgccgaa catgccaaag gactggatct
     4441 gcgccggttg gtgccgttcc gtctgccgcg ggcgacgcgc tgggcgctgg tgatcctcgc
     4501 gctgggggct gggctgggtt ttgtgcccga ataccggagc aaagcattcc tgcaaaagaa
     4561 ggccgacgcg cagaatatta aagaggtcgg caagcgcctg gcggacctga cccgccgcac
     4621 cgtggagaag cgtgcgcccg ccctcgaacc cacgcagaag gccatggaga ccgtcacgga
     4681 gttgggggac cagcttacca agcaagctct gacgcgcagc gaggcgctca aggacttggc
     4741 caacgtggcg gagaagctca agaacgagct taaggaaatg ggcaaggacc cggcgttaag
     4801 gaagatggaa caagccgcgc gggcttctac cgggaacgac tcccagacgg ccgccggttt
     4861 gcagaagcag atcgaggcca tgcagaagca gatgggcacg ccgaccggga accccgaggc
     4921 catcgacaag ctgaagaagg acttggagaa gcttcaggag gccgccaaag gcatggccaa
     4981 caagaatacg cccggcagcc aagcggaccg gcagaagatg tctgaatcgc tttccgcgct
     5041 ttccaagcag atgcaggaca tgggcctgca attgccgcaa ctggacgacg cgatcaaagc
     5101 gctggcggag aaccagaccg acctggtcct gaaggacttg gaggcttccc tcaccgacct
     5161 cgagaagatg cgcgacatgg ccaagtcgct gcagcagctt cagcagcagg cgacgaagct
     5221 gggcaaggat ttggccgagc agctcaagaa tggccaaccc gaacaagccc aggcgacgct
     5281 gcagaagatg gtggcgcaat tgaaattgcc caatctcgca ccggagcagc ttcagaagat
     5341 actggcggaa gtgtccaagg cggtcgatcc ggccggcaat tacggcaaag tggcggagca
     5401 cttgaagaac gccggccagc agatgcaggc ggggaacaag cccggcgcgg cgcaggcgct
     5461 ggcagcggcg gccaaggaat tggagaatct gacacagcaa atgggcgatg cgcaggcgtt
     5521 gatggcgacg ctcgacagcc tgaaccaggc ctcgatgtgc atcggctccg gccagggatg
     5581 ggggacgcat accggtaaca agccgggcta tagtcccttc ggcaagggac cgggcggggg
     5641 cgtcggcacc tggggtgatc ccaacgctca atgggacggc cagtggagcg accactggga
     5701 caattctggc gccaaccgac cggaccagga tgggatgggc cacaccgacc gcggcgaggg
     5761 tgagctgagc gatgcgctca agccgacaaa ggtaaaaggc caattcaagc ccggcggcca
     5821 gatgccgagc attaccctga aaggggtgag catcaaaggt cagagcaagg tggcctacga
     5881 ggaagccgcc accgccgcgc aatccgacgc ccagagcgcc ttgagccagg aaaaggtccc
     5941 ccgcgcctac cagggggcag tgaaagacta ttttgacgat cttaagaagt gaagatatga
     6001 ataccgaaga acaaatccaa accttccgcc aggcccattc cgcattgcgg gcggaactcg
     6061 gcaaggtcat cgtcggccag gattccgtcg tcgagggcac gctgatcgcg ctattcgcgg
     6121 gcgggcacgt gctgctcgaa ggggtgccgg ggctgggcaa gacgctgctc gtgcgcacgc
     6181 tcggcgaggt gctcgacctc tccttcagcc gcatccagtt cacgccagac ctgatgccag
     6241 ccgacattct cggcacgaac attgttatgg agatatccgg cgggcggcgg gagtttcagt
     6301 tccagcgcgg gccgatcttc gcgcacctgg tgctggcgga cgaaat
//
LOCUS       DATCGS010000408         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_332303, whole genome shotgun sequence.
ACCESSION   DATCGS010000408 DATCGS010000000
VERSION     DATCGS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332303"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /gene="lysS"
                     /locus_tag="VIL39_08350"
     CDS             <1..1788
                     /gene="lysS"
                     /locus_tag="VIL39_08350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5481475.1"
                     /translation="SQSRRTHYAQAILDDFPGHDGKPVTVAGRLMSWRKQGALAFGHV
                     QDQSGRIQLFLRRQLVQPTAAAEGILGYAEFNLLDMGDVVEATGKVMKTERGEISVLV
                     ENLRLLTKAIRPLPDQWSGLKDRELVLRKRYLDLILDRQPLERFASITRMVAAIRSFL
                     NGRGFMEFTTPIITPQYGGGTAKPFKTHVNALGCDMYLAISHELYLKRLIVAGFDKVY
                     TIGRYFRNEGIDRSHHPEFSMVETMTAYENYEYNMDLIEDMFRHIATTAFGRTEFKVR
                     GHVIDFSKPWRRVSMADAVKEKTGVDFRELKTVDEANARLAALGINEPQPSVGEALVK
                     AFEGVEPDLIQPTLVYGHPVEISPLAKPLAADPRFVERFEIFIAGMECGDNWSEQNDP
                     AHLLETWKKAYRSEERDSGKFHTLDFDFIEALEYGMPPTTGIGPGIERMAMIFTEQEI
                     IDDVIFFPMMRPSVSPVNASIYGIAEPTLAPVEDLALSVEDFEALCRDGVLKPHARHL
                     ILKPHVRLWSVAPAKSCGSSHVEIEGFLPSSVLRVAGYKIKTEEVLSDEEAKKQVLGL
                     MELSLVKFLQETFPACQVTVSPATVLHNA"
     gene            complement(1920..>2685)
                     /gene="galK"
                     /locus_tag="VIL39_08355"
     CDS             complement(1920..>2685)
                     /gene="galK"
                     /locus_tag="VIL39_08355"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY5481476.1"
                     /translation="VATATLLEAMTGQTLDPIEKAVLCQRAEHDFAGVPCGIMDQFTS
                     ILGQENHALLLDCRSRTTTPVAMTDPSVTVLIINTNIRHKLAESEYAKRRAQCEVAAR
                     ALKVPALRDATLEGLEAARGQLDPVVFRRARHVISENERTLQTARAIQASDWAAAGRL
                     MYASHASLRDDYEVSCPELDTVVEIAQAMGDEGGVIGCRMTGGGFGGCAVSLVKTNAV
                     QRITRSLDAAYEKKTGTQASIFSSRPAAGARILKSR"
BASE COUNT          483 a          863 c          844 g          495 t
ORIGIN      
        1 tcgcaaagcc gtcgtaccca ttacgcccag gccatcctgg acgacttccc cggtcacgac
       61 ggcaagccgg tgacggtcgc gggccgcctg atgtcgtggc gcaagcaggg cgcgctggct
      121 ttcggccacg tccaggacca gtccggccgt atccagttgt tcctgcgccg ccagctcgtc
      181 cagcctaccg ccgccgcgga gggaatcctt ggctacgccg aattcaacct gctcgatatg
      241 ggtgacgtgg tcgaagccac cggcaaggtg atgaagacgg agcgcggcga gatttccgta
      301 ttggtggaga atctgcgtct gctcaccaaa gccatccggc cgctgcccga ccaatggtcc
      361 gggttgaagg accgggagct ggtgctgcgc aagcgctacc tggatctgat cctcgaccgg
      421 caaccgctcg agcggtttgc ctctatcacc cgcatggtgg cggccatccg gtccttcctg
      481 aacgggcgcg gcttcatgga gttcaccacc ccgatcatca cgccgcaata cggcggcggc
      541 acggccaagc cgttcaagac ccatgtcaac gcgctcggct gcgacatgta cttggctatc
      601 tcccacgagc tttacctcaa gcgcctcatt gtcgccgggt tcgacaaggt ctataccatc
      661 ggtcgctatt tccgcaacga aggcatcgac cgcagtcacc accccgagtt ctcaatggtc
      721 gagaccatga ccgcctacga gaactacgaa tacaacatgg acctcattga ggacatgttc
      781 cgccacatcg ccacgaccgc gttcgggcgc accgagttca aggtgcgcgg ccatgtcatt
      841 gacttcagca agccctggcg ccgggtgagc atggcggacg ccgtcaagga gaagaccggc
      901 gtggacttcc gcgagcttaa gaccgtggac gaggccaacg cgcggctcgc cgctctgggg
      961 atcaacgaac cgcagcccag cgtcggcgag gcgctggtga aggcgttcga gggcgtggaa
     1021 ccggacctca ttcagcccac gctggtgtac gggcacccgg ttgagatttc gcccctggcc
     1081 aagccgctgg cggcagaccc ccgcttcgtg gagcgcttcg agatcttcat cgccggcatg
     1141 gaatgcggcg acaactggtc tgagcagaac gacccggctc atctgctgga gacctggaag
     1201 aaagcctacc ggtcggagga gcgggacagc ggcaagttcc acacgctgga ctttgatttc
     1261 atcgaggcgc tcgaatacgg catgccaccg acgaccggca tcggccccgg catcgagcgc
     1321 atggcgatga tcttcaccga gcaggagatt attgacgacg tgatcttctt tccgatgatg
     1381 cggccctcgg tgtccccggt gaacgcctcg atttacggca tcgccgaacc gacgctggcc
     1441 ccggtcgaag acctcgccct gtcggtggag gactttgagg cgctctgccg tgacggcgtc
     1501 ttgaagcctc atgcccgcca tctcatcctc aagccgcacg tgcgcttgtg gagcgttgcc
     1561 ccggccaagt cgtgcggctc cagccacgtg gagatcgagg gtttcctgcc cagcagcgtc
     1621 ctgcgggtgg cgggctacaa gatcaagact gaggaggtgt tgtccgacga ggaagcgaag
     1681 aagcaggtgc tgggcctaat ggaactgtcc ctggtcaagt tcctgcagga aaccttccca
     1741 gcctgccagg tcacggtctc cccagccacc gtgctgcaca acgcctaagc gctccgttct
     1801 agtgcttggt tgcagaggtt agcgatagcg ccgcgtgagg gcacgcggcc tacagaatcc
     1861 ttgtaggccg cgtgccctca cgcggcgaac cacgtaacgc ttgtttatgc aaccaagcac
     1921 taccttgatt tgaggatgcg cgcaccggcg gcggggcggg aggaaaagat gctggcttgc
     1981 gtgccggtct tcttctcgta ggccgcgtcc aggctgcgcg tgattcgctg gaccgcgttg
     2041 gtcttgacca ggctgacggc acaaccgcca aagcccccgc ccgtcatgcg gcagccgatg
     2101 acgccgccct catcacccat cgcttgggcg atttccacca cggtgtccag ctcggggcag
     2161 ctcacttcgt agtcgtcgcg gagggacgca tggctggcat acatcaaccg gccggcggcg
     2221 gcccagtcgg aggcctggat cgcgcgagca gtctggaggg tgcgttcgtt ctcgctgatg
     2281 acgtggcggg cgcgccggaa cacgaccggg tccaattggc cccgcgccgc ttcgagccct
     2341 tcgagcgttg catcgcgcag cgccggcacc ttaagggcgc gtgcggcaac ctcgcattgt
     2401 gcccggcgtt tggcatattc gctttccgcc aatttgtgcc ggatattggt attgatgatc
     2461 agcaccgtga ctgagggatc ggtcatcgcc accggggtag tcgtgcggga gcggcaatcc
     2521 agtaacagcg cgtgattctc ctggccgaga atggaagtga actggtccat gatcccgcac
     2581 ggcacgccgg caaagtcgtg ttcggcccgc tggcacagca ccgccttctc aatcgggtcg
     2641 agcgtttgcc cggtcatggc ttccagcaag gtggccgtgg cgact
//
LOCUS       DATCGS010000409         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_134781, whole genome shotgun sequence.
ACCESSION   DATCGS010000409 DATCGS010000000
VERSION     DATCGS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134781"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..388
                     /locus_tag="VIL39_08360"
     CDS             134..388
                     /locus_tag="VIL39_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481477.1"
                     /translation="MNRHNITLSEPVSEMVKAQVKNGRYKDFSAALQDAAWNYFYGPP
                     SPFEEYGVTPKEVERAAQRDLAAIRRDRKAGKLKLWKPGL"
     gene            385..657
                     /locus_tag="VIL39_08365"
     CDS             385..657
                     /locus_tag="VIL39_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481478.1"
                     /translation="MIYYQAGFERRYKKLAPQQRSRVDAAVARFEAKVGRPHEHSGIS
                     PRAFGRYVEFRAGLELRILALAEGGDFFLICVGNHDELRAYVRNNP"
     gene            complement(748..3312)
                     /locus_tag="VIL39_08370"
     CDS             complement(748..3312)
                     /locus_tag="VIL39_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEY5481479.1"
                     /translation="MNTTSSPSLPAIRLGWLLAVSAPGLPLWTASAAERFPFVVPGDD
                     ATASATDFSGLSPKAAGADGFVHIQDDHFFAGSSRLKIWGVNLCFGANFPTHEDAEKV
                     AAHLAKLGINGVRMHHHDTAAAPRGVWGPVTDGRRTLDPVMLERQDYLLDQLHRHGIY
                     ANLNLHVGRTFTAAEGFATKDLPASVQYSKYLIYFEPRMRELLKEFCRDYLTHTNKHR
                     GLSRASDPGIAVIELSNENSFSTLGPDIAASLPEPYRGEFKQQWNRWLIRRYPSTDAL
                     KKAWGTSLEPLGPSLVEPAKWQENLGGWRLNQSAEFPVKPHFDQAGPQPSLRALQLEI
                     PKAVAELHRQELQFPNLTLQPSRTYTLSFWLKADAARPLYVDVSNQGPDNWQGVGVAE
                     TVQAGPEWALVTRVFRSADKIPGKARICFKFGGNATGFSLAGARLQQGGEFIVVPAGQ
                     SLEKGDVEIPASTWCEAARRDARQFMADTEKGFIRELTAFLRKDLGVRVPITASQITY
                     HGAEIVAETCDYADIHAYWQHPHFPGRPWDPANWTIRNTPMEQAPDADSLLSRAPWRL
                     LDRPFTISEWNIPDPHDCAASTVPFAAMVAALQDWDGVFFFDYYSSESGWDTDRMRGY
                     FSFNGQPVKLALLAACANLYRRGDLKPLPDVAAGTLQDMLPATLGLGHRIGIDPKATL
                     PASLAAPTTKRLASPDGRAVWDAGERTTAHVLINTPNSRAVWGLIAGQKFDLGGIQLA
                     LGATEHNYAALIFTSLDGKPLEKARRILLAAVGSAENQGMEWNDTRTSVGNKWGTGPT
                     LVNGIAAEVTLPFRVKSVQALDGRGQPQGAVPVRVEGNTSRFTIGPEHRTLWYEIE"
     gene            complement(3392..>3627)
                     /locus_tag="VIL39_08375"
     CDS             complement(3392..>3627)
                     /locus_tag="VIL39_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481480.1"
                     /translation="LGENDAALNLWKQVTENHSYPRAKVQLAELYVTRNQPDLARPEL
                     KEVLADDAHGPAYQRRRDRVWVRRARSLMRKVG"
BASE COUNT          606 a         1055 c         1267 g          699 t
ORIGIN      
        1 acgctcgcgg cgtcgagagc caaagtggtt cgagccggtc aacgtggcgc aggtgtcccg
       61 cccggtaacc tacggcttgg ctagtcactc gtttggggtt gtctgcatgc ataacgtggc
      121 ataaagtgca tatatgaatc gtcataacat cactctgtct gagccggtga gtgagatggt
      181 gaaggcccag gtcaagaacg gacgctataa agatttctcg gccgcgcttc aggatgccgc
      241 gtggaactac ttttatggcc cgcccagccc ttttgaggag tacggggtga ctcccaagga
      301 ggttgagcgc gcggcccagc gggatctggc cgccattcgc agggatcgga aggctggaaa
      361 gctcaaactc tggaagccgg gtctatgatc tactaccaag cgggcttcga acggcgctat
      421 aagaagctcg cgccgcagca acgctcgcgg gttgatgcgg cggtggcacg ctttgaagca
      481 aaggtaggcc gaccgcatga gcacagcggg ataagcccgc gggcctttgg ccgctatgtg
      541 gaatttcggg caggcctcga actgcgcatt ttggcgctgg cggaaggcgg agactttttc
      601 ctgatatgcg tcgggaacca tgatgagctt cgcgcgtacg tccggaacaa tccttagttt
      661 ccggcgttcg ctacgtcatc cgggaaattt ggtgtgctct cgggggcgtg agccctctac
      721 cgctttggga tcagcagggt cccggcatca ttctatctcg taccaaaggg tgcgatgctc
      781 ggggccgatc gtgaagcgag aagtgtttcc ttcgacgcgg acgggcactg cgccctgcgg
      841 ttggccgcgg ccatcgagcg cttggacgct cttgacccgg aatggcagcg tcacctcggc
      901 tgcgatgccg ttgacgagcg tcgggccggt gccccacttg ttgccgaccg aggtgcgggt
      961 gtcgttccac tccatgccct gattctcggc ggagccgacg gcggccagca ggatgcggcg
     1021 ggccttttcc agtggtttgc catcgaggct ggtgaagatg agcgcggcat aattgtgctc
     1081 ggtggcgccc agcgctagct ggatgccgcc caggtcgaac ttctgcccgg cgatcaagcc
     1141 ccagacggcg cgcgagttgg gcgtgttgat cagcacgtgc gccgtggtgc gctcgccggc
     1201 atcccacacg gctcggccgt cggggctggc gagacgtttc gttgtgggcg cggccaggct
     1261 tgccgggagc gttgctttgg gatcaatgcc gatgcggtgg cccaggccga gtgtcgccgg
     1321 caacatgtcc tgcaatgtgc cggcggcgac gtccggcagc ggcttcaagt cgcctcgccg
     1381 atagaggttc gcgcaggcgg cgagcagggc gagcttcacc ggttggccat tgaaggagaa
     1441 gtagccgcgc atgcggtcgg tatcccagcc gctctcgctg ctgtaataat cgaagaagaa
     1501 aacgccgtcc cagtcctgca aggcggccac catggccgcg aagggcaccg tgctggcggc
     1561 gcaatcgtgg gggtcaggga tgttccactc cgagatggtg aagggccggt ccagcagccg
     1621 ccacggcgcg cggctgagca ggctgtcggc gtcgggcgcc tgctccatag gtgtgttgcg
     1681 gatggtccag ttcgccgggt cccagggccg cccggggaag tggggatgct gccagtaggc
     1741 gtggatgtcg gcgtaatcgc acgtctcggc cacgatctcc gcgccgtgat aagtgatctg
     1801 ggaagcggtg atcggcacac gcacgccgag gtcctttcta aggaaggctg tgagctcgcg
     1861 aataaagccc ttctccgtat ccgccatgaa ctggcgggca tcgcggcgcg cggcttcaca
     1921 ccaggttgac gccgggattt ccacgtcgcc cttctccagg ctttgcccag ccggcacgac
     1981 gatgaattcg ccgccctgct gcaagcgcgc gccggccaga gagaaaccgg tggcgttccc
     2041 gccgaacttg aagcagatgc gcgccttgcc ggggatctta tcggcgctgc ggaagacgcg
     2101 ggtgaccagg gcccactccg gcccggcctg gaccgtttcc gcaacgccca cgccttgcca
     2161 gttgtcgggg ccttgattgg agacatcaac atacagggga cgcgcggcgt cggccttgag
     2221 ccagaaggag agggtgtagg tgcgcgacgg ctggagtgtc agattcggga attggagttc
     2281 ctgccggtga agctccgcga cggccttcgg gatttcgagc tggagggccc gaaggctggg
     2341 ctgtgggccg gcttgatcaa agtggggctt cacggggaac tcggcgctct ggttcagccg
     2401 ccacccgccc aggttctcct gccatttcgc gggttcaacg aggctgggcc ccaatggctc
     2461 gaggctcgtg ccccaggctt tcttcaaggc gtcggtcgag ggatagcgcc ggatcagcca
     2521 acggttccac tgctgcttga actccccgcg gtatggttcc ggcaggctcg cggcgatgtc
     2581 gggcccgagc gtgctgaagg agttctcgtt gctcagttca atcacggcaa tgcccggatc
     2641 ggacgcgcgc gacaggccgc ggtgtttgtt ggtgtgcgtg agataatccc ggcagaactc
     2701 cttgagcagc tccctcatgc ggggttcgaa gtagataagg tacttgctgt actgcacact
     2761 ggcgggaagg tctttggtgg cgaacccttc cgcggcggtg aaggtgcgcc cgacatggag
     2821 attgaggttg gcgtaaatgc cgtgccggtg aagttgatcc agcaggtagt cctgccgttc
     2881 gagcatgacc ggatccagcg tccgccggcc gtcggtgact ggtccccata cgccgcgggg
     2941 cgcggcggcg gtgtcgtgat ggtgcatccg cacgccgttg attcccagct tggccaggtg
     3001 cgcggcgacc ttctcggcgt cctcgtgggt ggggaagttg gcgccgaagc acaggttcac
     3061 gccccagatc ttcaggcggc tggacccggc gaagaaatgg tcgtcctgga tatgcacgaa
     3121 gccatccgct ccggcggcct tgggcgaaag ccccgagaag tcggtcgccg acgcggtggc
     3181 atcgtcaccg ggtactacga agggaaaccg ctcagcggct gaggcggtcc aaaggggaag
     3241 cccgggcgcg gagactgcta atagccaacc gaggcggata gccggcaggc ttgggctgga
     3301 cgttgtgttc atgcgacgag gcttctacca tgccggaaga taagtttcca gctccatgcg
     3361 tcgaggcagg gatgtcttct ggaatactgg gctagccgac tttgcgcatc aggctcctgg
     3421 cgcgacggac ccagacgcgg tcgcgcctgc gttggtaggc cgggccatgc gcatcgtccg
     3481 ccaacacctc tttgagttcc ggccgggcga ggtcgggctg gttgcgggtg acgtagagtt
     3541 cggcaagctg gacctttgcg cgcgggtagg agtggttttc ggtcacttgc ttccagaggt
     3601 tcaaggcggc atcgttttcg cctagcg
//
LOCUS       DATCGS010000410         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_33072, whole genome shotgun sequence.
ACCESSION   DATCGS010000410 DATCGS010000000
VERSION     DATCGS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33072"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..2288
                     /locus_tag="VIL39_08380"
     CDS             114..2288
                     /locus_tag="VIL39_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481481.1"
                     /translation="MIYRFRHGVIGPLAIVWLSATVASVVIGAVAWSRFSRNIDAAAE
                     AAQLRESIDQLFSVLHDAGASERDYLLTGNAAYRKAFTNAERSFPAAFERIAAAAKHD
                     PVGQSDLIELRRLVELELADLRRAVALRAEKGPIVAELAASPGQTRTTMDRIREIIKR
                     RHDDQRDLMSARGEGTRREMKWVHQLTWIAGLLGVGAGVFALYFYQVDYYQERGRRQL
                     LEEKLHAEQSVREKSAFLANMSHEIRSPMNAILGFSELLEPDGLTPKQAQYVRAIRDS
                     GAALLRLINDILDLSKLEAGKLELYPDPTDMRDSCQFLRTVFGQQAITKSLQLQFELS
                     SNLPRALMLDRLRLRQVLVNLLSNAIKFTERGHVKTRVSWESQEDGRSGTLLIDVEDT
                     GIGIPAGELEEVFKPFVQAEARQPAEKEGTGIGLTIVERLTKLMGGSLTVNSEVGQGT
                     VFHLRFANVPVSGRLPVGDHAAPGGAVDFNDFAPATLLVVDDNPTNRAYMAGIFEKTH
                     HHVHFANNGREALASLGQAKPDVVLLDIRMPVMDGRTALAEIRKQASLASLPVIAVTA
                     SSKAGEEVELQRQFSGYIRKPFSRQTLFVALAQFLQRTSPGNGVEQPNLGQALKRIPL
                     PSPDQAAQWQKLAFELRRREAAEWPALRDSLAVNETRAFAHNLFTLGQAAYCAPLATY
                     AAALTTYADAYAISQMERHLAAFPKLVESIETAAARAELQPV"
     gene            2296..3426
                     /locus_tag="VIL39_08385"
     CDS             2296..3426
                     /locus_tag="VIL39_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY5481482.1"
                     /translation="MNAPLLETNTGCMLVVDDQESNIQVVGAALGKLGFEILPAAGGL
                     QAFQRLAVRRPDLILLDLLMPEMDGFEVCRRIRENPDWTEIPIIFLSSADDKGLIVRA
                     LESGGVDYITKPFNHAELVTRVRTHLALKRARDELKQLAEDRDELLGILTHDLKNHLG
                     GMDLSAQLLRDRAEAMADPKLRLMAENISHSSSQMLAFVKEFLANASADHGLSLKTEP
                     ISLKDAASRVVQQHGEAAKRKQLVFQTALEGDEALVQADLAALSQVLDNLLSNAIKFS
                     PPGKQIRVTVCPSGARYVECQVQDEGPGFTENDKTRMFRRYARLSARPTGGEPSTGLG
                     LSIVKKLMLAMQGELACESTPGNGATFAFRLPRAAPPTLTAK"
     gene            3423..>4192
                     /locus_tag="VIL39_08390"
     CDS             3423..>4192
                     /locus_tag="VIL39_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY5481483.1"
                     /translation="MKETILKKTIPKSTSPATQLPKCPTGIQGLDEITGGGLPRGRPT
                     LVCGGAGCGKTLLAAEFLVRGAALFGEPGVFMAFEETEAELKANVASLGFDLAGLVRR
                     KKIMLDYVHIERSEVQESGEYDLEGLFVRLNHAIDSIGAKRVVLDTLEVLFATLPNEA
                     ILRAELRRLFRWLKDKGVSAIITAERGRDQLTRHGLEEYVSDCVIVLDHRVFDQIATR
                     NLRVMKYRGAQHGTNEFPFLIGDEGISVLPITSLGLNH"
BASE COUNT          815 a         1277 c         1316 g          784 t
ORIGIN      
        1 ccgaaatatc cgagccgttt aataatgcct agacaccgtg cattttgcat ggtatgttga
       61 agcctccttc attggtcgga cgcgtgagcg ctcgacttga cgcagggaag cgcatgatct
      121 accgatttcg acatggggtt atcgggccat tagccattgt ttggctaagc gcgacggtag
      181 ccagcgtggt gatcggggcg gtggcttgga gccgcttttc ccgcaacatc gacgctgccg
      241 ccgaagctgc gcagcttcgc gagtccattg atcaactctt ttcggttttg catgatgccg
      301 gggccagcga gcgagattac ctgctgacgg ggaacgcagc ctatcgcaag gccttcacga
      361 acgcggagcg ttcatttccg gcggcattcg agcggatcgc cgccgccgcg aagcacgacc
      421 cggtggggca aagcgactta atcgagttgc gtcgcctggt ggagctcgag ctggcggatc
      481 tgcgccgggc cgtcgcgctg cgcgccgaga agggtcccat agtggccgag ctggccgcca
      541 gtccagggca aaccagaaca acaatggacc gcatccgtga aatcatcaaa cggcggcacg
      601 acgatcagcg tgatcttatg tccgccaggg gagaggggac ccgccgcgaa atgaaatggg
      661 tgcatcaatt gacgtggatc gccgggctgc tcggggtcgg ggcgggggta ttcgcgctct
      721 atttttacca agttgattac taccaggagc gagggcggcg gcaattgctg gaagagaagt
      781 tgcacgccga acaatcggtg cgggagaaga gcgccttcct ggccaacatg agccatgaga
      841 ttcgcagccc catgaacgcg attctcggct tcagcgaact gttggagccg gacgggctga
      901 cgccgaagca agcccaatat gtgcgggcca tccgcgacag cggcgcggcc ttgctgcgac
      961 tgatcaacga cattctcgac ctgtccaagc tcgaagcggg gaagttggaa ttgtaccccg
     1021 acccgacgga tatgcgcgat tcgtgtcagt tcctccgcac cgttttcggc cagcaggcca
     1081 taaccaagtc gctccagctt cagttcgagc tctcatccaa cctgccgcgg gcgttgatgc
     1141 tggatcgcct gcggttgcgc caggtgctgg tgaacttgct cagcaatgcc attaagttca
     1201 ccgagcgggg tcatgttaaa acgcgcgttt cctgggaaag ccaggaggac ggccgcagcg
     1261 gaacgctgct aattgacgtc gaggacaccg gcatcgggat cccggctggg gaactggagg
     1321 aggttttcaa acctttcgtt caggcggaag cccggcaacc ggcggaaaag gaaggaaccg
     1381 gcatcgggct aactatcgtc gagcgcctca ctaagttgat gggcggcagc ctgaccgtca
     1441 acagcgaagt tggccagggg acggttttcc acctgcgttt cgcgaatgtt cccgtctcgg
     1501 ggcggttgcc ggtgggagat cacgccgcac cgggcggagc ggtggacttc aatgacttcg
     1561 cgccggccac gctgctggtg gtggatgaca acccgaccaa tcgggcctac atggctggca
     1621 tatttgagaa gacccatcac cacgtccatt ttgccaataa cggtcgggaa gcgttggcat
     1681 ctctggggca agccaagccc gatgtggtct tgctcgatat ccgaatgccg gtgatggacg
     1741 ggcgcacggc cctggcggag atccgcaagc aagccagtct ggcgtccttg ccggtcatcg
     1801 cggtcaccgc ttcgagcaag gccggcgaag aggtggagct gcaaaggcag ttcagcggct
     1861 acattcgcaa gcccttctcg cggcaaacgc tctttgtggc gttggctcag tttctccaaa
     1921 ggacgtcgcc cgggaatgga gtggagcagc cgaatcttgg gcaagccctc aagcgcattc
     1981 ccctcccttc gcctgaccag gccgctcagt ggcagaaact cgctttcgaa ctgcgccgcc
     2041 gggaggcagc cgaatggccg gcgttgcgtg acagcctggc ggttaatgag acgcgggcct
     2101 ttgctcacaa ccttttcact ctcggccagg cggcctattg cgctccgctg gcgacttatg
     2161 ccgccgcgct taccacgtat gccgacgcct acgccatcag ccagatggaa cgtcatctgg
     2221 ccgcgttccc caaactggtt gagtccatcg agaccgctgc ggcacgcgcc gaactgcaac
     2281 cggtatgagt tcccaatgaa cgctccctta ctagaaacaa acacgggctg tatgctcgtc
     2341 gtggatgacc aggagtccaa catccaggtc gtcggcgccg ctctgggtaa actcggtttc
     2401 gagatcttgc ccgcggccgg cgggctccag gccttccaac gactggccgt gcgccgcccc
     2461 gacctcatcc tgctcgacct gctaatgccc gagatggacg gattcgaggt ttgccggcga
     2521 attcgcgaga acccggattg gactgagatt cccatcattt tcctttcctc ggccgacgac
     2581 aagggcctga tcgtgcgcgc cctcgagagc ggcggcgtgg attacatcac caagcccttc
     2641 aaccacgccg aattggtaac gcgcgtccgc acccacctgg cgctcaaacg ggcccgcgac
     2701 gagttgaagc agcttgccga ggaccgggac gagttgctcg gcatcctcac ccacgacctc
     2761 aagaaccatt tgggcggcat ggacctgagc gcgcaattgc tccgtgaccg tgccgaggcg
     2821 atggcggacc cgaagctgcg gctgatggcc gagaacatct cacattcaag cagccagatg
     2881 ctcgccttcg tgaaggagtt tctggccaat gcttcggccg accacggcct gagcctcaag
     2941 accgagccga ttagtctcaa ggacgcggcc tcccgggtcg tccagcaaca tggggaagcg
     3001 gcgaagcgca agcaactcgt ctttcagacg gcgctcgagg gggacgaggc tctcgtgcag
     3061 gcggacctcg ccgcgctcag ccaggtgctg gataatctcc tgtccaacgc catcaagttt
     3121 tccccgcccg gcaagcagat tcgcgtcacg gtgtgtccgt cgggtgccag gtacgtggaa
     3181 tgccaggtcc aggatgaagg gcccgggttc accgagaacg ataagacccg gatgttccgc
     3241 cgctatgcgc gcctttccgc gcggcccact ggcggcgagc cctcgaccgg cctgggccta
     3301 agcatcgtca agaagctcat gctggccatg cagggtgagt tggcttgcga aagcacgccg
     3361 ggcaacggcg ccaccttcgc ctttcgtctg ccccgcgctg cccccccaac actgactgct
     3421 aaatgaaaga gaccattctg aaaaagacta ttccaaaatc cacctctcca gcgactcagt
     3481 tgcccaagtg ccccacgggc atccagggtc tcgacgaaat caccggcggc ggtttgccgc
     3541 gcggacggcc tacgctggtc tgcggcggcg cggggtgtgg caagacgctg ttggccgcgg
     3601 aattccttgt gcgcggcgcg gcgctgttcg gcgagccggg cgtgttcatg gcctttgagg
     3661 agacagaagc ggagttgaag gccaacgtcg cgtcgctcgg atttgatttg gcgggtctgg
     3721 tccggcgcaa gaaaatcatg ctcgactacg tccacatcga gcgcagtgaa gttcaggaga
     3781 gcggcgagta tgacctggaa gggttgttcg tccggctcaa tcacgcgatt gattccatcg
     3841 gcgccaagcg cgtggtgctg gacacgctgg aagtgctgtt cgccaccctg cccaacgagg
     3901 ccatcctgcg cgccgagttg cgccggctct tccggtggct caaggacaaa ggcgtgagcg
     3961 ccatcatcac ggccgaacgc gggcgcgacc aactgacgcg gcacgggctg gaggaatacg
     4021 tgtccgactg cgtcatcgtg ctcgaccatc gggtctttga tcagattgcc acgcggaatc
     4081 tgcgcgtgat gaaatatcgc ggcgcgcagc acggcaccaa cgagtttccc tttctcatcg
     4141 gcgacgaggg catcagcgtg ctgcccatca cgtcgctggg gctgaatcac aa
//
LOCUS       DATCGS010000411         4458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_84718, whole genome shotgun sequence.
ACCESSION   DATCGS010000411 DATCGS010000000
VERSION     DATCGS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84718"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VIL39_08395"
     CDS             complement(<1..425)
                     /locus_tag="VIL39_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481484.1"
                     /translation="MKRHILFGTLALLAGSLIAANAAPKDDVSAAAKKLADKDNYSWK
                     QTTENAGGGGFGGGASEGKTEKDGYLWLSMTMRDNTIEAVKKGDKGAIKTADGWQSLS
                     EATCGDPGPATFAARRLQSFKAPTAQAGELADKVKELKL"
     gene            complement(635..3391)
                     /locus_tag="VIL39_08400"
     CDS             complement(635..3391)
                     /locus_tag="VIL39_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5481485.1"
                     /translation="MNAALLYVVRNLLRRRTRTVLGALGIFLTLALLTAIQIGLDSVS
                     ISYLDLVALQAGKADLVIAKPGGDPLNPLPFDPAAVRSRLEQNPHLRGLSPRWFGLVQ
                     VQVQGEQHYAVLIGLDPQRERELDLSGLVPEPTLQGEVCAISKALADRLKVKPDSKLS
                     VHSLSTGAAVDLRLESVLQRQLLLPQQFRDYIVVNQAAAQALLQEPERVHALAGAFRN
                     PHSYYDARDLHSSVLRLKNAGAAIAADLGMNYELRLPKAAAITVFEDFTSPLRAVFGV
                     FALLALSITGLLLYSLISVAVEERIREYAILRTLGAKQRDIFRLVLTESFLLCFLGVV
                     PGVLGGVVCAEVIVKLIGLAMGAKGAALPLELSAHTLLLTLAGGVALSIGSALVPALH
                     ATRWRIVDALDPLRRGQIPAGPAPEGQVNRPLVLTGLVLSALSVVVFFVLPTALLSGN
                     PSLIGTLVLCLLVSILLGFTMALVGVLPFVQRALLLGLGWLFGPVAELAGRNLERHRR
                     RHTTTALLFTLSVSLVIFIASLVALASRTALALVEHTHGADLKIQAFHTGSESLKPEL
                     ERIEGVKAVSEVRFLHSRSDAGIAYDVVISDVVGMKNLWVVPFGADRDLAKVLYTRQI
                     VCEAGRPEAIAELAAQAVNPAEKASPSNALPPVVLSLAVARFLEVDAGDLVQLSFRLG
                     SERSDARFRVAAVCSAMPGFESFRARVARAVGAGVLMSLDNFKTMTRSAPAEAFQGLY
                     FARTTGDASAQKTAAHRIREEFDVRYRFGVQCTAEQKEQARILYWATQVLFGLLLVVA
                     VLIAVFALIASMATTVMERRREIGVLKALGLRRRQLFLLFLAEAVILTLSAGIAGGAI
                     GFTLAWLFVLQASVLMELATAFTMPYVTFLATLAISILAGALAAYVPTRSLLRKSAAE
                     ILRI"
     gene            complement(3388..4080)
                     /locus_tag="VIL39_08405"
     CDS             complement(3388..4080)
                     /locus_tag="VIL39_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481486.1"
                     /translation="MNEIAVHAENLHKSYLLGATAVGALRGVDLTVRRGEFVAFMGPS
                     GCGKTTLLNLIGAIDTPSRGRLYVDDVALERLSEDELADLRRDRIGVVFQFYNLLPTL
                     SARENIELPMQFKGTKRRQRRERALWLLERVGLKDRAEHKPAELSGGEQQRVSIARAL
                     ANQPALVLLDEPTGDLDSATGQEIMALLRDLNQREKVTLLVATHDPLVAQASSRIIRI
                     RDGRVESDSKAA"
BASE COUNT          884 a         1357 c         1429 g          788 t
ORIGIN      
        1 tccagcttga gttccttgac tttgtcggcg agttccccgg cttgggcggt cggagccttg
       61 aagctttgca gccggcgcgc ggcgaacgtg gccggacccg ggtcaccaca ggtggcctcg
      121 ctgagcgact gccaaccgtc cgcggtcttg atggcgccct tgtcgccttt cttcactgcc
      181 tcgatggtgt tgtcgcgcat ggtcatcgag agccagaggt agccgtcctt ctcggttttg
      241 ccttcggagg ccccgccacc gaatccgcct ccgccggcgt tctcagtcgt ctgtttccag
      301 gagtagttgt ctttgtccgc gagctttttg gccgcggcgc taacgtcatc cttgggggcg
      361 gcgttcgctg caatcagcga gcccgccagc aaagccagtg tgccgaatag aatgtgtctt
      421 ttcatacttt gtttttgcgt ttggtttgtt ttgttgactg ctattaccgc tcgaaccgac
      481 caattgatag atggcctgaa tgctggcgcg cgcgcaacca aattgctacg cccaccagcg
      541 aatgaacata tatcgagaac atcctaacac ctaagcctgt ggagagcaaa tcgtcagcag
      601 tgagacccct tgcaacaccg tggccactgc tttttcagat gcgcaggatc tcagccgccg
      661 acttgcggag caggctgcgg gttggcacgt aggcggcgag cgcgccggcc aggatgctga
      721 ttgccagagt tgcgaggaat gtcacgtaag gcatggtgaa ggccgtcgcc aactccatca
      781 agaccgaagc ctggagtacg aaaagccagg ccagcgtgaa tccaatcgcg ccccccgcga
      841 tgccagccga cagggtgagg atcaccgctt cggccaggaa caggagaaag agctggcgcc
      901 ggcgcagccc aagcgctttg agcacgccga tttcccggcg acgctccatc accgtggtgg
      961 ccattgacgc aatcagcgcg aaaacagcaa tcagcacggc caccaccaac agcaacccga
     1021 aaagaacctg ggttgcccag tagagaatcc gggcctgctc cttttgctcg gcggtgcact
     1081 ggactccgaa acgataccgc acgtcgaact cttcccggat gcggtgggca gcggtctttt
     1141 gggcggacgc atcgccggtc gtccgggcga agtacaggcc ctggaaagct tcggccggcg
     1201 cggagcgcgt catggttttg aagttgtcca gggacatcag cacgccggcg cccaccgcgc
     1261 gggcgactcg cgcgcggaag ctctcgaagc ccggcatcgc gctgcatacg gcagcgaccc
     1321 ggaatcgggc gtcgcttcgc tctgagccca gtcgaaagga caattgcacc aggtcgccgg
     1381 catctacttc gagaaaccgt gcgacggcca ggctgaggac caccggcggc aaggcgttgg
     1441 agggcgacgc tttctccgcc gggttcaccg cctgggcggc gagttcggca atggcctccg
     1501 gccggcccgc ttcgcacacg atttggcggg tatagaggac cttcgccagg tcacggtccg
     1561 cgccaaacgg cacgacccac agattcttca taccgacgac gtcgctgata acaacatcgt
     1621 aagcgatgcc ggcatccgag cggctgtgga ggaacctgac ttccgaaact gctttgacgc
     1681 cttcgatgcg ttcgagttcg ggtttcaggc tttcgcttcc ggtgtggaag gcttggatct
     1741 tcaagtccgc tccgtgggtg tgctcgacca gggccagggc ggtgcggctg gcgagcgcga
     1801 cgaggctggc gatgaaaatc accaacgaaa ccgacagggt aaaaagcagg gcggtggtgg
     1861 tgtggcggcg ccgatggcgt tcgaggttgc gcccggccaa ctcagccaca ggtccaaaga
     1921 gccaaccaag cccgagcagc agcgcccgct gcacgaacgg taacacccca accagagcca
     1981 tcgtgaagcc cagcaggata gacaccagca ggcaaagcac cagggtgcca atcagcgatg
     2041 gattaccgga cagcaaagcc gtcggcagga caaagaacac cacgactgac agcgcggaca
     2101 ggacgagtcc cgtcaggacg agcggacggt tgacctggcc ttcgggcgcc gggccggcag
     2161 gaatctggcc ccgccgcaaa gggtcgaggg catcaacaat ccgccagcgg gtcgcatgca
     2221 gggcggggac gagggcgctc ccgatggaaa gcgccacgcc gccagcgagc gtgagcaaga
     2281 gcgtatgggc gcttaattcc aaaggaagcg ccgctccctt ggcgcccatg gccagcccga
     2341 tgagttttac gatgacctcc gcacaaacga cgcctcccag cacgcccggc actacgccca
     2401 ggaagcaaag cagaaaggat tcggttaata ccaggcggaa gatgtcacgc tgcttggctc
     2461 ccaaggtccg caggatcgca tattcccgga tgcgctcctc cacggcgacc gagataagcg
     2521 agtaaagcaa caggccggtg atgctcaggg ccagcagggc gaacacgcca aaaacggcgc
     2581 gcagaggcga ggtgaagtcc tcgaagacgg tgatggcggc cgctttgggc agtcgcagtt
     2641 cgtagttcat gcccaggtcg gcggcgattg cggcgccggc gttcttgagc cggagcacgc
     2701 tcgagtggag gtcgcgcgca tcgtaatagc tgtgtggatt ccggaaggcg ccagccaggg
     2761 catgcacgcg ctcgggttcc tgcagaagcg cctgggcggc agcctgattc accacaatgt
     2821 agtcgcggaa ttgttgcggg agcaggagtt ggcgttggag aacgctttcc agccgcaaat
     2881 caactgccgc gccggtgctg agagaatgaa cggagagttt gctgtccggt ttgactttga
     2941 gtctatccgc cagggccttg gaaatagcgc agacctctcc ctgcagagtc ggctcgggaa
     3001 ccagcccgga taagtcgagt tcccgttcgc gctgcgggtc aagcccaatc aacaccgcat
     3061 aatgctgctc gccctggacc tggacctgga ccaagccgaa ccacctgggt gagagcccgc
     3121 gcaaatgggg attctgttcg agccgggagc gcaccgcggc agggtcgaaa ggcaggggat
     3181 tcagcgggtc gccgcctggt ttggcaatga cgaggtcggc tttcccggcc tgcaacgcca
     3241 ccaaatcgag ataggagatg gagaccgaat cgagcccaat ctggatggcg gtcagcaggg
     3301 ccagggtcag gaagatgccg agcgcgccaa gcaccgtgcg agtgcggcgc cggagcaggt
     3361 tgcgcacgac atacagcagg gcggcgttca tgcggccttc gaatcacttt cgactcgtcc
     3421 gtctcttatc cggatgatgc gggagctggc ttgggcgacg agcggatcat gcgtcgccac
     3481 cagcagcgtt actttctccc gctggttcaa gtcccggagc agcgccataa tctcctggcc
     3541 cgtagcgctg tccaggtcgc cggtcggctc gtccagcagc accagcgccg gctggttggc
     3601 cagcgcgcgg gcgatcgaaa ccctctgctg ctcgcccccg gagagctccg cgggcttgtg
     3661 ctcggcgcgg tcctttagtc ccactcgctc gagcagccac aatgcgcgtt cgcgccgctg
     3721 ccgtcgcttg gtgcctttga actgcatcgg cagctcgatg ttctcccggg ccgagagcgt
     3781 tggcagcaag ttgtaaaact ggaatacgac gccaatgcgg tcgcgccgca aatccgccaa
     3841 ctcgtcctcc gaaagccgtt cgagcgcgac gtcatcaaca tacagccggc cgcgtgaggg
     3901 ggtgtcaatc gcgccaatca gattcagcag cgtcgtcttg ccgcagccgg agggacccat
     3961 gaaggcgacg aactccccgc ggcggacggt gagatccacg ccgcgcaagg cccctaccgc
     4021 ggtagcgcct aaaaggtaac ttttgtggag attctccgcg tggacggcaa tttcgttcat
     4081 agccttgcct gagagtggtg aggtcagcca ggcttgtcga gatggaactg gagccgcaca
     4141 cagctcgaac cgctcaagct caccctcgcg cacgtgtgca ttcttcgcgc catcaacagc
     4201 gagcgcggca ttcacccgcg gagcgctgag tacatgtggg gtagccttgg tgtagccttg
     4261 gtgtagcctt ggggtagcct tggggtagcc ttggggtagc cttggggtag ccttggggca
     4321 cggctccggc ggatcattgc ataaggtgtt gcagatgaag cggatctaag atttggcggg
     4381 acaattcgcc tcgaggctgc caaacttggc gccatgattt gtcctgacaa gatacaacgc
     4441 atcgcgaaac ctgaggac
//
LOCUS       DATCGS010000412         4350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_332632, whole genome shotgun sequence.
ACCESSION   DATCGS010000412 DATCGS010000000
VERSION     DATCGS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332632"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VIL39_08410"
     CDS             <1..744
                     /locus_tag="VIL39_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5481487.1"
                     /translation="ARNGRGAEAELDGHHYFVGNHRFTHELAVCSEDIERKLAEIEAQ
                     AQSVVVIGHKPHGDCKGEVLGVLAVGDAIRPNAAEAVRSLHAAGILQVVMLSGDNQRT
                     VDAIAHQVGIDEARGDLLPEQKVDRVRELMARHKHVGMIGDGVNDAPAMAVASVGIAM
                     GAAGTDTAIEIADMALLQDDLSKVAGAIRLGRRTVRVIQANIAFALGVKAIFLALALT
                     GHTSLWLAILADTGATLVVIANALRLLRR"
     gene            complement(785..1063)
                     /locus_tag="VIL39_08415"
     CDS             complement(785..1063)
                     /locus_tag="VIL39_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY5481488.1"
                     /translation="MKHGHDLEERRALVVRLRKIRGQLQAIEKMVEAETDCAEVLTQV
                     VSARRALKSFADTLIQSHLHDCIEHAETQAESRKKLRALLTVLERYVA"
     gene            1062..2270
                     /locus_tag="VIL39_08420"
     CDS             1062..2270
                     /locus_tag="VIL39_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5481489.1"
                     /translation="MPGLYHGDLPAASPNAVDTVWGMGVYACVNSFQDYLAHGQGWLF
                     LPAAVLLGALHGLEPGHSKTMMASFIISIRGTVAQAALLGLSAAFSHTLVIWVLAAIG
                     MHFSGKLDVEGLEPWFQLATGAIIVGMAAWMFARIRRGQRAASHQHHADEPQPPATQG
                     AQGGPLLATGANERVQISVFETDVPPRFRLYFYDQRQQPISPPAGQTLTLETSRPDGT
                     KQVFEFTPQGDYLESTSNIPEPHEFNVGLKVSTGGYPITYYTSFVEHHHHSHDATQDG
                     FEDAHQREHAQQLQSRFAGQNVTTRQVVLFGLTGGLMPCPAAFAVLLLCLQIKQFALG
                     FATVLAFSIGLAATLVAVGSAAALSVREASRRFAGFAAFAHRLPYVSVGLIGFVGLVL
                     AVLGLKHISQ"
     gene            complement(2272..2853)
                     /locus_tag="VIL39_08425"
     CDS             complement(2272..2853)
                     /locus_tag="VIL39_08425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5481490.1"
                     /translation="MKVRESGMPEEAYWETLFDVPLILDRLGIDARLKNVVELGCGYG
                     TFTVPVARRISGTVTAFDIDEAMIERTRQRAAAAGARNVLCVVGDVFANGFGGGVDSQ
                     DACLLFNILHCEEPVRLLAEAARVVRPGGAVLVIHWRCDPATPRGPSMEIRPRPEQIA
                     GWARETGLLEAEGPVIDLPPWHYGLRLRREVRR"
     gene            3012..3749
                     /locus_tag="VIL39_08430"
     CDS             3012..3749
                     /locus_tag="VIL39_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5481491.1"
                     /translation="MNPADRGIALAAPCTFNRSAAEGVVPALEKIVAAIRQRCRKARI
                     IVRGDSGFGREEIMAWCEGQQAVVYYCLGLAKNGILIERLALALADARARRCLSGAAQ
                     VRVIREFEYRTQTSWSCWQRVIGKAEVMAAGDNPRFVVTNLPAEGFKGQEDRLSTHYL
                     ASNQLRLWLATLGYLLLERLRRLGLAGTDLARATVGRVRLKLLKAAAQVRVSVRRVYV
                     QLSSAYPLQALFRLGHRRLMRLAPASG"
     gene            3780..4025
                     /locus_tag="VIL39_08435"
     CDS             3780..4025
                     /locus_tag="VIL39_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481492.1"
                     /translation="MYQAPREKCLQNGPKSTGCETIEKYNLTYRVWDNGDWFVVDGKE
                     RATYDSVPAGPVFRADGVLEFLAANRPSLYRIEVTDF"
     gene            complement(4057..>4350)
                     /locus_tag="VIL39_08440"
     CDS             complement(4057..>4350)
                     /locus_tag="VIL39_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481493.1"
                     /translation="EAERLLARGLAALGLAEGQLAETRKGAWEKEVLACWLCQHTTAR
                     RRWVSERLGMGDESRVTQAIGRLKRKEQLELERLKRRLEQVYEDHNGEVEVRI"
BASE COUNT          778 a         1449 c         1277 g          846 t
ORIGIN      
        1 gcgcgcaacg gacgcggcgc ggaagcggaa ctggacggcc accattactt cgtcggcaac
       61 caccgcttca cccacgaact ggcggtttgc tccgaggaca tcgagcggaa gctcgccgaa
      121 atcgaagccc aggcgcaatc ggtggtcgtc atcggccaca agccgcacgg ggactgtaaa
      181 ggcgaagtgc ttggcgtgct ggcggtcggc gacgccattc gcccgaacgc ggccgaggct
      241 gtccgctcac tgcacgctgc gggcatcctg caagtcgtga tgctcagtgg cgacaaccaa
      301 cgcaccgtgg acgccatcgc ccaccaggtg ggcattgacg aggcgcgcgg cgacttgctg
      361 ccggagcaaa aagttgaccg tgtgcgagag ctgatggccc ggcacaaaca cgtcggcatg
      421 attggcgacg gcgtgaacga cgcgcccgca atggccgtgg ccagcgtcgg catcgccatg
      481 ggcgcggccg ggacggacac cgccattgaa atagcggaca tggcgttgct gcaggacgac
      541 ttgagcaaag tcgccggggc gattcggctg ggccgccgca ccgtgcgcgt catccaggcc
      601 aacatcgcct ttgcgctggg agtgaaggcc attttcctag ccctggccct gactggccac
      661 acgagcctct ggctcgcgat tctggccgac accggcgcga cactggtcgt cattgccaac
      721 gccctccggt tgcttcgaag atgaaattcg ccgccgggaa tacatcagta accgcgccgg
      781 ctggctaagc cacgtaacgc tccagcaccg tcagcaacgc gcgcaacttc ttgcgcgatt
      841 ccgcctgcgt ctccgcgtgc tcgatgcaat cgtgcaggtg cgactggatc agcgtgtccg
      901 cgaatgattt cagtgcgcgg cgggctgaaa ccacctgcgt cagcacctcc gcgcagtccg
      961 tctcggcttc caccatcttc tcaatcgctt gaagctgacc ccggattttg cgcaaccgca
     1021 ctaccagggc tcttctttct tccagatcgt gaccgtgttt catgccaggc ttataccacg
     1081 gcgacctgcc ggccgccagc cccaatgccg ttgacacagt atggggtatg ggggtatatg
     1141 cctgcgtgaa ttcattccag gattacctcg cgcacggcca gggctggctg ttcctgcccg
     1201 ccgcggttct gctcggtgcc ctccatggcc ttgagcccgg ccacagcaag acgatgatgg
     1261 cctccttcat catctccatt cgcggcaccg tcgcccaggc cgccctgctc ggcctgtccg
     1321 ccgccttttc ccataccctc gtcatttggg tgctggcggc catcggcatg catttttccg
     1381 ggaaactcga tgtcgaagga ttagaaccct ggtttcaact cgccaccggc gcgatcatcg
     1441 tcggcatggc agcctggatg ttcgcccgca tccgtcgcgg ccagcgcgct gcctcacacc
     1501 agcatcatgc ggacgagccc caacccccgg caacgcaggg cgcgcagggc ggccccttat
     1561 tggcgaccgg tgccaatgag cgagtccaaa tcagcgtctt tgaaactgac gtgccacctc
     1621 gttttcgcct ctacttctac gaccagcgcc agcaaccgat ctcgccgcct gctggccaga
     1681 ctctcacttt ggaaacctcg cgacccgatg gcactaaaca ggttttcgag ttcacccccc
     1741 aaggagacta cttggagtcc acctcaaaca ttcccgaacc ccacgagttc aacgtcggcc
     1801 tcaaagtttc caccggcggc taccccatca cctactacac ctcgtttgtg gaacaccacc
     1861 accattccca cgacgcgacc caggacggct ttgaggatgc ccaccagcgc gagcacgccc
     1921 agcaactgca atcccggttc gccggtcaga acgtgacgac caggcaagtt gtgctcttcg
     1981 gactgaccgg cggcctcatg ccctgcccgg cagcgttcgc ggtgcttttg ctctgcctgc
     2041 aaatcaaaca attcgccctg ggcttcgcca ccgtcctggc cttcagcatc ggcttggctg
     2101 ccaccctggt cgctgtcggt tcagccgccg ccctctccgt ccgtgaagcc agccgccgct
     2161 tcgccgggtt tgctgccttt gcgcacaggt tgccttacgt cagcgtcggg ctcattggct
     2221 tcgttggcct cgtgttggcg gtgctcgggt tgaaacatat ttcgcagtaa attaccgcct
     2281 tacctcgcgc cgcagccgca aaccatagtg ccacggcggc aaatcaatca ccggcccctc
     2341 ggcctccagc aaccccgttt cccttgccca cccggcgatc tgctccggcc tcggtcgaat
     2401 ctccatgctc ggcccccgcg gcgtcgccgg gtcgcaacgc cagtgaatca ccagcaccgc
     2461 gccccctgga cgcaccaccc gcgccgcctc agccagcaaa cgcactggct cctcgcaatg
     2521 cagaatgttg aacagcaggc acgcgtcctg gctgtccacc ccgccgccaa aaccattggc
     2581 aaacacatcg cccacgacac acaacacgtt ccgcgctccc gctgccgccg cccgttgccg
     2641 cgtgcgctcg atcatcgcct catcaatatc aaacgccgtc accgtgccgg aaatccgccg
     2701 cgccaccgga accgtgaaag tcccgtaacc gcaacccagc tccacgacgt tcttgagccg
     2761 ggcgtcaatt cctaaccggt ccagaatcag cggaacatca aacagcgttt cccagtacgc
     2821 ttcttctggc atcccgcttt cgcgcacttt catggcaaga gttctgttcg gctatttggc
     2881 gcggtggcgg atttcgttcc atgaaagcgg catgggagag ttttactcgc acggcgggcg
     2941 gctgtcagta gggatttgcg cgccaggcgc gcggggcgca tcccttgggg cgtcttgctc
     3001 aaataggtcg gttgaacccg gcggatcggg gcatcgcgct ggcggcgccg tgtacgttca
     3061 accggagtgc ggccgaggga gtggttccgg ccttggagaa gatcgtggca gcgattcggc
     3121 agcggtgccg gaaggcacgg atcattgtgc gaggcgacag tggttttggt cgggaggaga
     3181 tcatggcgtg gtgcgagggc cagcaggcgg tagtctatta ttgcttgggt ttggccaaga
     3241 acggcatcct gatcgagagg ttagccctcg ccttggccga tgcgcgggcg cgccggtgcc
     3301 tgagtggtgc ggcccaggtg cgggtgatta gggagtttga gtatcggacc caaaccagtt
     3361 ggagttgctg gcaacgagtg attggcaagg ccgaagtgat ggcggcggga gacaatccac
     3421 gtttcgtggt gaccaacctg ccggccgagg ggttcaaagg ccaggaggac cggctgagca
     3481 cgcattacct ggccagcaac caactgcgtt tatggctggc caccttgggc tatctgttgc
     3541 tggagcggtt gcgcaggctg ggtttggcgg gaacggactt ggcgcgggcg acggtgggcc
     3601 gggtgcgttt gaagctgctc aaagcggccg cgcaagtgcg ggtgagcgtg cgccgggtgt
     3661 atgtgcaact gagcagcgcc tatcccctcc aggcattgtt tcgcctcggt caccggcggc
     3721 tgatgcgtct ggcgccggcc agtggctgaa gcggagttgc cgaatttcca aaccgcagag
     3781 tgtaccaagc gccacgggag aagtgtcttc agaatgggcc gaaaagcacc ggttgcgaga
     3841 caatcgaaaa gtacaacctg acctaccggg tgtgggataa cggtgactgg tttgtggtgg
     3901 atggcaagga gcgagcgacc tatgattccg tgccagcagg tccggtattc cgggctgacg
     3961 gcgtgttgga gtttctcgcc gcgaatagac cctcccttta ccgaattgaa gtgacggact
     4021 tttagcacat atggctcagt gaaagtcccg gaattctcat attcgcacct ccacttctcc
     4081 attatggtct tcatagacct gctccagtcg ccttttcagt cgctccaatt ccagctgctc
     4141 tttccgcttc agccggccaa tggcctgcgt cactcgggat tcgtctccca tccccaaccg
     4201 ctcgcttacc catcgccgcc gcgccgtggt gtgctggcac aaccaacacg ccaacacctc
     4261 cttttcccaa gctcccttcc tcgtctccgc cagttggcct tctgccagtc ccagcgccgc
     4321 caacccgcgc gccagcaacc gctcggcttc
//
LOCUS       DATCGS010000413         4869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_481592, whole genome shotgun sequence.
ACCESSION   DATCGS010000413 DATCGS010000000
VERSION     DATCGS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481592"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VIL39_08445"
     CDS             complement(<1..656)
                     /locus_tag="VIL39_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEY5481494.1"
                     /translation="MTKRTAFLALLVLNAVALCPAAETNLVANGSFEQSQSKPGVPDD
                     WSSAGNPAIQQQLTLDTGRDGQRCAKLACTEFNGDGPDFHVMICQIGKVSVRQGQWYR
                     ITFWAKAEGIQGGAIEVALSDTRRWANAGLEEVFSPGAQWAQFEFVFRARADLDASAS
                     RLQIWFKGTGTLWLDDVVLAESAGGQEWFPQISTEGVKNFVPNSSFECGTANWGSFTY
                     G"
     gene            complement(727..996)
                     /locus_tag="VIL39_08450"
     CDS             complement(727..996)
                     /locus_tag="VIL39_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481495.1"
                     /translation="MHLMLSHHRISGKSCFRDTAFIPIRAQLPARRGHAFMLDDQRST
                     KRVTERPQTGACLHCHAANTLAYRQEGLKAGTPGTLEAPLLSSEG"
     gene            995..1228
                     /locus_tag="VIL39_08455"
     CDS             995..1228
                     /locus_tag="VIL39_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY5481496.1"
                     /translation="MRTTLHIEDDVLQAAKELAQRNGGTAGQVISDLARRGLNAPPTK
                     RRPGLRGGVPVLPSRGEIITPEHVQKMQDEEGV"
     gene            1232..1666
                     /locus_tag="VIL39_08460"
     CDS             1232..1666
                     /locus_tag="VIL39_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEY5481497.1"
                     /translation="MRALLDVNVVIALLDPDHAFHERAHVWWAAHAKSGWASCPIVEN
                     GVVRIMSHPGYSQGARFTPGDLISRLRTFATQSNHEFWPDDVSLRDAALFTVERIHSS
                     RQLTDLYLLALASKHAGRLATFDQGISISAVCGAKAASLCVI"
     gene            1725..2714
                     /locus_tag="VIL39_08465"
     CDS             1725..2714
                     /locus_tag="VIL39_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5481498.1"
                     /translation="MNILFLGGTGNISAECAALLHERGHEILVLSRGRNAVPPGYRAI
                     QADRKDAAAMRAGLKGVQPEVVINFIGYDVPDLQVDYELFNGAVRQYVFISSTAIYAK
                     PPRQLPLTEDAPVGNPFWDYAGKKLACEQWLRQRHEETGFPVTVVRPSHTYSKRWIPN
                     PVSSGSYTFAARLEQGKPVYIHDNGESPWTLTAAADFAVGLGGLVGKTEAIGEAFHIT
                     SDEVLTWNQIYAEIAAALGVKSPQIVKVPTDFICQVAPQMTGTLKGDKAHPGVFDNAK
                     IKRFVPEFRCGKPFRIGVRESVSWLRAHPEQQNRKPELDVMIERVLEAWGRRK"
     gene            2711..3493
                     /locus_tag="VIL39_08470"
     CDS             2711..3493
                     /locus_tag="VIL39_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY5481499.1"
                     /translation="MRPKQNELISEALIAAARRLRAETGRLRFAAPVTHVYNPLAYAW
                     APHEAYLGKFGGARKRVVFLGMNPGPFGMAQTGVPFGEVAAVRDWLKIEYPVGKPERE
                     HPRRPVAGFACVRSEVSGRRLWGLFAERFGVPEQFFAEHLVMNYCPLAFLEDGGRNCT
                     PDKLPVSERAALFAACDRHLRAVVAALQPEWLIGVGDFATKRAEYLFPDGPLKIGRIL
                     HPSPASPAANRDWKGLVTRQLEQLGVWTRATESPPRGFHPRR"
     gene            complement(3453..3989)
                     /locus_tag="VIL39_08475"
     CDS             complement(3453..3989)
                     /locus_tag="VIL39_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481500.1"
                     /translation="MRSHVFRVLSVALLLTGCATQSKVEKAQHHTVYNKPLTSPGAKF
                     GALPPVVQRTVLAEAGPAEVVDAVRDPSSGRVVYKIYFRDADIFPPIYVAPDGSVLNP
                     DLTVAVSAFHGIRVKPDEVPATVKKVIPERAPSSEVAYIDLETWGNRTVYVVTFKDEA
                     HNPRLLIASDGSLVEETQ"
     gene            4202..4277
                     /locus_tag="VIL39_08480"
     tRNA            4202..4277
                     /locus_tag="VIL39_08480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Lys,seq:ctt)
BASE COUNT          948 a         1457 c         1556 g          908 t
ORIGIN      
        1 ccgtaggtga agctgcccca gttggcggtg ccgcactcga agctactgtt cggcacgaag
       61 ttcttcacgc cctcggtgga gatttggggg aaccattcct gcccgccggc cgattcggcc
      121 agcaccacgt cgtccagcca caacgtcccc gtgcccttga accagatttg cagccggctg
      181 gcgctggcgt cgaggtcggc ccgggcgcga aacacaaact cgaattgcgc ccactgcgct
      241 cccggcgaga acacctcctc cagcccggca ttcgcccacc ggcgcgtgtc cgagagggcc
      301 acctcgattg ccccgccctg gatgccctcg gccttcgccc aaaacgtgat tcgataccac
      361 tggccctgac ggacgctgac cttgccgatc tggcaaatca tcacgtgaaa gtccggtccg
      421 tcgccgttga actcggtgca ggccagcttc gcgcagcgct ggccgtcgcg tcccgtgtcc
      481 agagtaagtt gttgctggat cgccgggttc ccggcggatg accagtcgtc cggcacgcct
      541 ggcttgcttt ggctttgctc aaaggacccg ttggcaacta ggttggtttc cgccgcgggg
      601 cagagcgcca cggcattcaa tacgagtaag gccaggaagg cagtgcgttt cgtcataaac
      661 acgagtcaga ccgtcgaacc gacaccgagg atagaacaat ggcggccacc acggcaacga
      721 ttgaacctag ccctcggaac tcaggagagg ggcttccagg gtgccggggg tgccagcctt
      781 gagtccttcc tggcgatagg cgagagtgtt ggcggcgtgg caatgcaggc acgcgccggt
      841 ctggggacgc tcggtaacgc gtttggtgga acgctggtcg tccagcatga acgcgtggcc
      901 tcggcgcgcc ggcagttgcg cccggatcgg gatgaacgca gtgtcccgaa agcagctctt
      961 gccgctgatg cggtgatgtg atagcatcag atgcatgaga acgactttgc acatcgagga
     1021 tgacgtgctg caggcggcaa aggaactggc ccaacgcaac ggcggcaccg ccggccaagt
     1081 aatttctgat ctggcacggc gtggtttgaa tgcgccgccg acgaagcggc ggccgggcct
     1141 gcgcggtggt gtcccggtgc tgccttcgcg cggcgagatt atcacgccgg aacatgtgca
     1201 gaagatgcag gacgaggagg gcgtataacc catgcgcgcc ttgctggatg tgaacgtcgt
     1261 catcgcgttg ctggatccgg atcatgcgtt ccatgagcgg gcgcacgttt ggtgggcggc
     1321 gcatgcaaaa tccggctggg caagctgccc tatcgtggag aatggagtcg tccgcatcat
     1381 gtctcatccc ggctacagcc agggggcacg gttcacaccg ggcgatttga tctcacggct
     1441 gcgaacgttt gccacacaaa gcaatcacga attctggccg gacgatgtct cgttacgcga
     1501 cgcggcactt ttcacggtcg agcgcattca cagttcgcgg caacttacgg atctttatct
     1561 gctcgcattg gcgagcaagc acgccggcag gctggcgacg tttgaccagg gaatctccat
     1621 ttccgccgtg tgcggcgcca aagcggcaag cctatgtgtt atttgagcag gccggctgtc
     1681 atggtgccag gattggcacc ttaaacgcct cgcgggtatt acttatgaac atcctttttc
     1741 tcggtgggac gggcaacatc tcggcggagt gcgccgcgct gctgcatgaa cgcggccacg
     1801 aaatcctcgt gctgagccgg ggccggaacg ccgtgccgcc cggttaccgg gccatccagg
     1861 ccgatcgcaa ggacgctgcg gccatgcgcg cggggctgaa gggggtgcaa cccgaggtgg
     1921 tcatcaattt catcggctac gacgtgcccg acttgcaggt ggactacgag ctgttcaacg
     1981 gcgccgtgcg ccaatacgtg tttatcagct cgacggccat ctatgccaag ccgccccggc
     2041 agttgccgct aaccgaggac gcgccggtgg gcaacccatt ttgggattac gcagggaaga
     2101 agctggcatg cgaacaatgg ttgcggcagc gccatgagga aaccggcttc ccggtgacag
     2161 ttgtccgccc ctcgcacacc tactcgaaac gttggattcc gaacccggtc tccagcggaa
     2221 gctacacgtt cgcggcgcgg ttggagcagg gcaaaccggt gtacatccat gacaacggcg
     2281 agagcccgtg gaccctgacc gcggctgccg acttcgccgt cgggctgggg ggcctggtgg
     2341 ggaagactga ggccattggc gaagcgttcc acattacgag cgacgaggtg ctcacctgga
     2401 accaaatcta cgcggagata gcggcagcgc tgggggtgaa gtccccgcag atcgtgaagg
     2461 tgccgaccga tttcatctgc caggtggcgc cgcagatgac cggcacgctc aaaggcgaca
     2521 aggcgcatcc gggcgtattc gacaacgcga agatcaagcg ctttgtcccg gagttccggt
     2581 gcggcaaacc gttccgcatc ggcgttcgcg agtcggtgag ctggctgcga gcgcatccgg
     2641 agcagcagaa ccggaagccg gagctggatg tgatgattga gagggttctg gaggcgtggg
     2701 gaaggcggaa gtgagaccga agcagaacga actcatcagc gaagcgctca tcgcagcggc
     2761 cagacggttg cgcgcggaaa ctggccggct gagatttgcg gcaccggtca cgcatgttta
     2821 taacccgctg gcctatgcct gggcgccaca tgaggcatac ctggggaagt tcggcggcgc
     2881 acggaagcgc gtcgtgtttc tcgggatgaa tccgggacca tttggaatgg cgcagacggg
     2941 agttccgttc ggcgaagtgg ctgccgtgcg cgactggctc aagatcgaat atccggttgg
     3001 caagccggaa cgcgagcatc cgcgacggcc agtcgcaggg tttgcctgcg tgcgttcgga
     3061 ggtcagcggc cggcgtttgt ggggtctctt cgcggaacga ttcggggtgc cggaacaatt
     3121 ctttgccgag catctcgtga tgaactattg ccccctggct ttcctggaag acggcggtcg
     3181 caactgcacg ccggacaaat tacccgtctc cgaaagggca gccctcttcg cggcctgcga
     3241 ccgccatttg cgagccgtcg tcgcggccct tcaacccgaa tggttgattg gagtcgggga
     3301 ttttgccacc aaacgggcgg aatacctctt tccggacggc cccttgaaga tcgggcgtat
     3361 tctgcatccc agcccggcga gcccggcggc gaaccgcgac tggaagggcc tggttacgcg
     3421 gcaactcgag cagctaggcg tctggactcg ggctactgag tctcctccac gaggcttcca
     3481 tccgaggcga tgagcagcct cggattgtgc gcctcgtctt tgaaggtgac gacgtaaacg
     3541 gtgcggttgc cccaggtttc cagatcgata taggcaacct cggaagacgg cgcgcgctcc
     3601 ggaatcacct tcttgacggt agccggcacc tcgtcgggct tgacccgaat cccgtggaat
     3661 gcgctgacgg caacggtgag atcggggttg agcacgctcc cgtccggggc gacgtagatg
     3721 ggcgggaaga tgtctgcgtc tcggaaatag atcttataga ccacgcgacc cgagctgggg
     3781 tctcttaccg catcgaccac ttccgccggg ccggcctcgg ccagaaccgt gcgctgcacg
     3841 acgggcggca acgctccgaa cttagcgcct ggggaggtga gcggtttatt atacacggtg
     3901 tggtgttgcg ccttttcgac cttgctctgc gttgcgcacc cggtgagcaa aagcgcaacc
     3961 gacaacactc ggaaaacatg cgatctcata aacgtagagc aatgtatccg ccgcgccgca
     4021 gatagcaagc gtccgaactc ccatcacccg tgcgaacttg gctcgcttgg aactccttgc
     4081 tctcaggcct caccggatag cggccgcgaa atggccgccg aatcaacttg cctttctgct
     4141 tcgtttgtgc caaatagaac tccgtgctgg ctgctttgca gcaagcgcaa tcaacttggt
     4201 tgcctcggta gctcaattgg tagagcagtt gactcttaat caattggttc taggttcaag
     4261 tcctagccgg ggtaccaact acagccgtcc tcttccaaag ccccaggatt agcccctagg
     4321 cgagtacaaa tccggggtga aaaagtaata ggctttcggc caagaggtgg cattctcctt
     4381 caagttcaag tgatgaaacg tccaatgcga acattgattt gcatgctggc agcggccttg
     4441 ctggcaggcg ggtttccatt cggcagcgag gcaaaaagtt acagcagcgg cggcggccac
     4501 agctactcct cgcacagctc cagttccgga gggagccaca gctttagttc aggcggcagc
     4561 catagttcca gttcgggagg cagtcacagt ttcagctcac gcagcagtca cagttcgagc
     4621 ggagggtccg cgcacagctc aggcgccggc agcgggagcc atagcgccag ctcgggcggc
     4681 agcggcggcc agagccacgg gaccagcgtg agcccgggat ccagctcagg aagcagcagc
     4741 ggcaagaact tcagctccgg cagcggcaag agttacagtt ccggctccac ctcgagcgat
     4801 aataaccggc acagctacac ttccgccaag agttacagtt ccggcgcggg ccatactttt
     4861 acctccagc
//
LOCUS       DATCGS010000414         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_33351, whole genome shotgun sequence.
ACCESSION   DATCGS010000414 DATCGS010000000
VERSION     DATCGS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33351"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /gene="infB"
                     /locus_tag="VIL39_08485"
     CDS             complement(<1..1999)
                     /gene="infB"
                     /locus_tag="VIL39_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5481501.1"
                     /translation="MPVRIYDISKKLGLENKEVLAKAKALGLTAARVASSSLDKITAE
                     YLFEEILKERPELAAPPPPPPPPPPPPPPPTPPPSVVQAPIDQAVVTPAPAPPPEVET
                     VPKQEALVPTSPVALEPAAAAPPEAPPPVTPAATPVPVSAISEPQPEPKAEPAIEAPP
                     PPPPPPAPPAPPPSPKVGDKVGFIQLPPRSGARPGEKPGGVKLPPRGHEQRRSEFPRR
                     GDNRNSRGGTTAAAGPAMSGTRSPGQQKAVGVAKPASLSSATPKVALPADAQVITIKP
                     PIVVRDLAEQLKQKPFKIIADLMEAGVFASVNQAIDEKVAQRICAKYGFRFEVEKRER
                     GSGLVHTPTKTVELDVEDKTEDLVPRAPVVTIMGHVDHGKTSLLDVIRKSNVAAGEAG
                     GITQHIGAYTISFPHPERKKELAQITFLDTPGHAAFSSMRARGANVTDLVVLVVAAND
                     GVMPQTLEALSHARAAKVPLLVAVNKIDHPNANVLKVRQQLQDKGLVPDDWGGDTIFV
                     EVSALTKQGVDKLIEMILLQADLLELKANPNRHAKGNVIESGLEPGGPTATVLVRKGT
                     LRVGEIIICGRYYGRVRALISEEGKRLKVAGPSVAVKVLGLNGVPEAGLEFSVVEDEK
                     AARDLAEQRAQEAKALGLETNRTKVTLENLFDQLAETTSKVL"
     gene            complement(2088..3368)
                     /gene="nusA"
                     /locus_tag="VIL39_08490"
     CDS             complement(2088..3368)
                     /gene="nusA"
                     /locus_tag="VIL39_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY5481502.1"
                     /translation="MNADFLAVLEFWEREKGINRDVLSSAVQEALLSAAKKAVGPARE
                     LRVVIDQKSGDIRAFAKLVVSEKVISKHDQISVFDARRIKAEAQPGEEVEVEVTPVGF
                     GRIAAQYAKQALMQHIRRAEKQLIFAEFKDRVGDIISGTVRRFDRSDVFVDLGKHEAL
                     LPNRERVPTEEYQIGERIRCYVKAVEDGPHGPEIILSRADPRFVIKLFQLEVSEINDN
                     TIEIKGIAREPGFRTKLAVWTRDEKVDPVGACVGLRGQRVKNIVRELNNEKVDIIRWD
                     SNVKNFITNALAPAKLKAFQIDETRKRVRITVSEDQLSLAIGKRGQNARLTAKLSGWQ
                     VDIEPEVVVVRGFEEKVAEAVESLAAIAGITREQADVLVHHGLTRLEDLLQADESDLA
                     GIPELGDQAPAIMEAARTEAARRTIKVGEAPVTG"
BASE COUNT          588 a         1102 c         1078 g          752 t
ORIGIN      
        1 tgagcacctt ggaggtggtc tcggcgagct ggtcgaatag gttctccagc gtcaccttgg
       61 tgcggttggt ttccaagccc agcgctttgg cttcctgggc gcgctgctcg gccaggtcgc
      121 gcgcggcttt ctcgtcctcc acgacgctga attccaggcc agcttcgggc acgccgttca
      181 agccgaggac cttcaccgcc accgaggggc cggccacctt gaggcgtttg ccctcttcgc
      241 tgattagcgc ccgtacccgg ccgtaatacc ggccgcagat gattatttct ccgacgcgca
      301 gcgtgccttt gcgcaccagc accgtggccg tggggccgcc cggctccaag ccggattcga
      361 tcacattgcc tttggcgtgc cggttcggat tggccttgag ttccagcagg tcggcctgga
      421 gtaaaatcat ttcgatgagc ttgtccacgc cctgtttggt cagcgcggac acttccacaa
      481 aaatcgtgtc gccgccccaa tcatccggga ccaacccctt gtcctgcaat tgctggcgga
      541 ctttcagcac gttggcgttc ggatggtcga tcttgttgac tgccaccagc agcggcacct
      601 tggccgcgcg ggcatggctg agcgcttcga gggtctgcgg catgacgccg tcattggcgg
      661 ccaccaccag caccaccagg tcagtcacgt tcgccccccg cgcccgcatg gaactgaagg
      721 cggcgtgacc gggggtgtcg aggaatgtga tctgggcgag ctctttcttc cgctcgggat
      781 gcgggaaaga gatggtgtag gcgccgatgt gttgggtgat accgccggcc tcgccagcgg
      841 caacgttgga cttgcggatt acatcaagca ggctggtctt gccgtggtca acatgaccca
      901 tgatcgtcac gaccggtgcg cgcgggacca agtcctctgt cttgtcctcc acatccagtt
      961 ccaccgtttt ggtgggagta tggactaacc cgctgccgcg ttcgcgtttc tcgacctcaa
     1021 aacggaagcc gtacttggcg cagattcttt gtgccacctt ctcgtcaatg gcttggttga
     1081 cgctcgcaaa aacccccgct tccatcaggt cggcgataat cttgaatggt ttctgcttga
     1141 gctgctccgc caggtcacgg accacgatcg gtggcttgat ggtaatcacc tgggcatcgg
     1201 cagggagcgc cactttcggc gtggcgctcg acaggctggc gggtttcgcg acgcccacag
     1261 ccttttgctg gccaggggac cgggtcccgg acatagccgg gccggcggct gcggtagtgc
     1321 cgcccctcga attgcgatta tctcctcgcc gggggaactc tgatcttcgt tgctcgtgcc
     1381 cgcgcggtgg cagtttaact ccgccgggtt tctcgccggg tctggccccg gacctcggcg
     1441 gcaattggat gaatccaact ttatctccca ccttggggct gggcggcggc gctggtggtg
     1501 ctggcggtgg tggtggcggc ggtggcgcct cgatggcagg ctcggccttt ggttccggct
     1561 gaggctcgct gatagcgctg accgggaccg gcgttgcggc cggggtcact ggtggcgggg
     1621 cctcgggagg ggcggcggcg gcgggttcca gcgcgaccgg agaggttggc acgagggctt
     1681 cctgcttcgg gacggtttcg acctcgggcg gaggcgcagg cgcaggggta acgaccgcct
     1741 ggtctatcgg cgcttgaacc acggacggtg gcggagtggg aggtggcggt ggtggcggcg
     1801 gcggaggagg tggaggcggc ggcgcagcga gttcgggacg ctccttgagg atctcctcaa
     1861 agaggtattc ggcagtaatc ttatccagcg aactggaggc gacccgcgcg gcggtaaggc
     1921 ctaacgcttt cgccttggcg agaacttctt tgttctccaa gccgagcttc tttgaaatgt
     1981 cgtaaatccg aacgggcata attctttatc gcctcgggcc ctgattgcca cacagccttc
     2041 atgggctcga tcctaacaca caaggatttc cggcggcagc gcctcaatca accggtcacc
     2101 ggcgcctcgc cgactttgat ggtgcgccgg gctgcctcgg tacgcgccgc ttccataatg
     2161 gccggggcct gatccccaag ttccggaatc cccgccaagt cgctctcatc cgcctgcaac
     2221 aaatcttcca gccgagtcag accatgatgc accagcacgt cggcctgctc ccgcgtaatc
     2281 ccagctattg ccgccaggga ttcaaccgct tcggccacct tctcctcgaa acctctgacc
     2341 accaccactt cgggctcaat gtccacctgc cagccgctca gtttcgcggt cagccgcgcg
     2401 ttttggcccc gcttgccaat cgccagggaa agctggtcct cgctgaccgt gattctgacc
     2461 cttttgcggg tttcatcaat ctgaaaggct ttgagcttgg ccggggccag cgcattggta
     2521 atgaaattct tgacgttcga gtcccagcgg atgatatcaa ccttctcgtt gttcagctcc
     2581 cgcacgatgt tcttgaccct ttgaccgcgc aagccgacgc aggcgcccac ggggtccacc
     2641 ttctcatcgc gggtccagac cgccagcttg gtccgaaagc ccggctcgcg ggcgatgccc
     2701 ttgatttcga tggtgttgtc gttgatttct gacacttcga gctgaaacaa cttgatgacg
     2761 aagcgcgggt cggcgcgcga caaaatgatt tccggaccgt gcgggccgtc ctcgacggcc
     2821 ttgacgtagc aacggattcg ctcgccgatc tgatattcct cggtcggcac gcgctcacgg
     2881 ttgggcagca gggcctcatg cttgcccaaa tccacgaata cgtccgaacg gtcgaaccgc
     2941 cggacggtgc cgctgatgat atcgcccacc cggtccttga attccgcgaa aataagctgc
     3001 ttctcggcgc gccggatgtg ctgcatgagg gcctgcttgg catactgggc cgcgatgcgg
     3061 ccgaaaccca ccggggtgac ttctacctcg acctcttcgc caggctgggc ctccgccttg
     3121 atccgccggg cgtcgaacac ggaaatctgg tcatgcttgg agatgacctt ctcggacacg
     3181 accagcttgg caaaggcgcg aatatcgcca ctcttctggt caatcaccac gcgcaactcg
     3241 cgcgctggtc ccactgcctt cttggcggcg gacaaaagcg cttcttggac cgcagagctc
     3301 aggacatctc gattgatgcc cttctcgcgt tcccagaatt ccaggactgc taaaaagtcg
     3361 gcgttcataa gtcgtggacg gcagtcccct cagggggaac aaaaaagtcg gcagattaag
     3421 ctcttccgac tttcgcgcgc tggcaaacca gcaaataccg ttctattcat aaacttaaga
     3481 gtttggtgga aaacagtcaa gctccgattc gtgcctccgg
//
LOCUS       DATCGS010000415         6248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_33401, whole genome shotgun sequence.
ACCESSION   DATCGS010000415 DATCGS010000000
VERSION     DATCGS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33401"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VIL39_08495"
     CDS             <1..281
                     /locus_tag="VIL39_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY5481503.1"
                     /translation="FERVHELTKAHTEFASTPVSAETLGIPRYLWEDGKLLADDARAL
                     AAAIAHEKAKVEFYRTLGDAALIPAVRTTLRALADEEADHMRRLQALA"
     gene            complement(419..1822)
                     /locus_tag="VIL39_08500"
     CDS             complement(419..1822)
                     /locus_tag="VIL39_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY5481504.1"
                     /translation="MIVPGVALRKDANRFVRLVLGDVFLGFLAIVAAALTIVPSLFKV
                     SPQLDALLEAGQWTIVALFAVEYGNGLAAASSKGKFIRNPWRIIDAATILIPLLTLVP
                     GASRFLRSSAMLRLIRLAGVVALGARASGVRTREEKAAMALQDRAPMQVRMLPATAGT
                     PSRSASWEEFVHWAKKPGTEWYSISNVGPHNLAELAKAAGISQEFIAAHLLGTSYPHL
                     ETTDRYLSLFVWLPERSAANQIERNGLLLLASDKSVVTFCRRSVHLMEKIAGPVTGEL
                     ASLPFPARTLCQFLKTVLDANEALIGRFEKELGELEDFPVRDSRQQFFERTFRLKKEL
                     STAQSDLWRLRGVLKELAEPRFNVPGRTAGEIPFLHDLVEAGDYLYETVNNVREGVLS
                     LIELHLNVVSFEMNRVMRVLAVVSVLGLIPAVVGGLLGMNLADNPWPVTLPQVTFAVC
                     FAMVTSLYFFVAKGWLR"
     gene            complement(2099..2497)
                     /locus_tag="VIL39_08505"
     CDS             complement(2099..2497)
                     /locus_tag="VIL39_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY5481505.1"
                     /translation="MKKQVTITAEPINEPALLSQRVMSPGMGAVVCFLGVVRGTEGDA
                     TISALEYEVFQSMAEHQINLLFDEMARRWPIESVRLVHRIGLVKANEPSLWVEVIAPH
                     RGEAFAACQWLIDELKRVVPIWKKPVMGGP"
     gene            complement(2510..2755)
                     /locus_tag="VIL39_08510"
     CDS             complement(2510..2755)
                     /locus_tag="VIL39_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5481506.1"
                     /translation="MRVTVCFYSYFKDLTGCASTTEELADGSTLDDLFKTLVVRFPKL
                     GAMQRATLMAVGLDYQNRTYVLSEGDEVSLFPPVQGG"
     gene            complement(2785..3435)
                     /gene="hpt"
                     /locus_tag="VIL39_08515"
     CDS             complement(2785..3435)
                     /gene="hpt"
                     /locus_tag="VIL39_08515"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY5481507.1"
                     /translation="MRAPVPEINIASRARVPTRWRKEVERILITDDQIAQRIRSMSRG
                     IERDFTGREMVVVSLLNGTVMFLADLIRNLSLPLRLDFIGVSSYGAGTESGDLVFTKE
                     LRLGVRGRDVLLVDDILDTGKTLSRVLGKLRALKPRRIKTCVLLNKAARRVEEIEADY
                     VGFEIPDFFVVGYGLDFAERYRNLPFVGVLHPHVYKKACQRVIPAKPKRAGGRRTV"
     gene            complement(3502..3882)
                     /locus_tag="VIL39_08520"
     CDS             complement(3502..3882)
                     /locus_tag="VIL39_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HEY5481508.1"
                     /translation="MEQPDQLHELFRMQKALNERIGVQMEGMSVEDKTKWLLNYCRAM
                     SQEIAELTDSVPWKWWAKYQKFDEQNARVEVVDLLHFLISLAQVLGMSADDVFNAYIQ
                     KHKVNFQRQESGYTVKDEHDSKHI"
     gene            4006..4635
                     /locus_tag="VIL39_08525"
     CDS             4006..4635
                     /locus_tag="VIL39_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5481509.1"
                     /translation="MPKLPELLSKGGAPLWLLLLVSAVAVAVFIERFLHCHRAQINST
                     EFLNGVRTVLKRNNVVEALSICDATPGPIARLVKVAILNRDHGRERVREALEEAGAAE
                     VPRLEEKLNLLATIAQLAPLIGLLGTVLGFMKTFGEMQDDGLNAHIGKLAEGVWTALV
                     CAAAGLAVAIPTHAGYNYLVSRINSIVLDMERSATEIVQIVTEDVKPQP"
     gene            4638..5078
                     /locus_tag="VIL39_08530"
     CDS             4638..5078
                     /locus_tag="VIL39_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5481510.1"
                     /translation="MRFPRNAHILRSQLDAAPFAAVLFLLVIFMMLGSLVYTPGARLE
                     LQLPQANGLPGTDKPTVSVAIDADGRLYYENQWIEESNLRRRLQEAVKKSAEPLALVV
                     QADKAVSYEKCLRLALLAHDAGLSEAILATLPRPYATTPPRSPQ"
     gene            5075..5866
                     /locus_tag="VIL39_08535"
     CDS             5075..5866
                     /locus_tag="VIL39_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5481511.1"
                     /translation="MTSELVESRPWSRPHWWGMVGLIFGVQLVLIFWLGETSPIRPRP
                     AAPALTLKLAGSASAELLALHDPTLFVLPHPQEVLAPTWLRTPPPEFHSFAWPEPTNN
                     PLLAIDQLGAGFNRLVETTPFNSLPPPAKPLPELTLPELPPLAISTPHSTLRLEWDQA
                     RRQLLTPLELRSWQNPDILTNSVVQIVVDAEGRPGSVTLLSGSGSKAADQQALEQANA
                     SRFEPLSHNLAGPAFNPAAQLTWGRMIFQWHTLPAQPTNPPAAGS"
     gene            5869..6093
                     /locus_tag="VIL39_08540"
     CDS             5869..6093
                     /locus_tag="VIL39_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481512.1"
                     /translation="MSIANLILAYVIVLLAGITGVGIYSELRRRRFEPSPSEDHIFRC
                     RRCGYVYTDDPDVDRSRCTQCGQMNKAIEF"
     gene            complement(6116..>6248)
                     /locus_tag="VIL39_08545"
     CDS             complement(6116..>6248)
                     /locus_tag="VIL39_08545"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5481513.1"
                     /translation="SGMGYLGAANLAQLREQARFIRVSPAGQREAAPHDVMELKTGS"
BASE COUNT         1208 a         1993 c         1884 g         1163 t
ORIGIN      
        1 attttgagcg cgtacacgaa ctgacgaaag cacacacgga gttcgcttcg acaccagtgt
       61 ccgctgagac gctgggcatt ccacggtatt tgtgggaaga cggcaaattg cttgccgatg
      121 atgcgcgggc gctagccgcc gccattgcgc acgaaaaagc caaggtggaa ttctatcgca
      181 cgctgggcga tgccgcgctg attcccgccg taagaactac tctgcgtgcc ctggccgatg
      241 aagaagccga ccacatgcgc cggttgcagg ccctggcata agtctatcac cgggtccgag
      301 ccggactggc gggggccgtc cgctgacagc acgcaggagg aactggtggc cgcccgggaa
      361 gctcgccggg ttgtggcggc cttagtgccc ggtgacggaa atacggtgac gtagcacgtc
      421 agcgcaacca gcctttcgcg acgaagaaat agaggctggt caccatggca aagcaaactg
      481 cgaacgtcac ctgcggcaag gtgaccggcc acggattatc ggcgagattc atgcccaaca
      541 gcccgcccac caccgccgga atcaggccca ggacactgac cacagccagc acgcgcatca
      601 cccggttcat ttcaaacgag acgacgttca agtgcagttc gatgagcgac aagacgccct
      661 cgcggacgtt gttgaccgtt tcgtagaggt aatcgccggc ttcgaccagg tcgtgcagga
      721 acgggatctc gccggcggtg cggccaggca cattaaaccg cggctcggcc aattccttca
      781 gcactccccg cagacgccac aagtcggact gggcggtgga aagctccttc ttcaagcgga
      841 acgtgcgctc gaaaaactgc tgccggctgt cccgcaccgg gaagtcctcc agttcgccca
      901 gttccttttc aaaccggcct atgagcgcct cattggcatc gagcaccgtc ttgagaaact
      961 ggcacagcgt gcgagccgga aacggcagcg acgccagctc gcccgtcacc gggccggcga
     1021 tcttctccat caaatgaacc gaccggcggc agaaggtgac cacgctcttg tcgctggcca
     1081 gcagcagcaa gccgttccgc tcgatctggt tggcggcgga ccgttccggc aaccaaacga
     1141 atagggaaag gtaacggtcg gtggtttcga ggtgcgggta gctggtgccc agcagatgcg
     1201 cggcgatgaa ctcctgcgag atgcccgccg ccttggccaa ctcggccaga ttatgcgggc
     1261 cgacattgga aatgctgtac cattccgttc ccggcttctt cgcccagtgg acgaactcct
     1321 cccaggaggc cgaacgggac ggtgtgccgg ccgtggcggg cagcatgcgc acttgcatgg
     1381 gagcccggtc ttgcagggcc atcgccgcct tctcctctcg cgtcctgacc ccgctggcgc
     1441 gcgccccaag cgcgaccacc cccgccaaac ggatcagccg cagcatcgcg gaggaacgca
     1501 ggaaacgcga ggcgcccggc accagtgtca acagcgggat gaggatggtg gcggcgtcaa
     1561 tgatgcgcca cggattgcga atgaacttcc cttttgagct ggccgcggcc agcccgttgc
     1621 cgtattcgac ggcaaagagc gcaacgatgg tccactggcc cgcttccaac agggcgtcga
     1681 gctgcggaga gaccttgaag agcgacggga cgatcgtgag cgccgccgcg acaatcgcca
     1741 ggaacccaag gaacacatcg ccgaggacaa gacgcacaaa gcgattcgcg tccttgcgca
     1801 gggcaacacc gggaacaatc atattctctg tcacagccaa ctatttgaac atgacgcgtg
     1861 ccattgtgcg atgctgaatc ccaagccttt ggaccacagc cttttacgac tattggctgc
     1921 tggcgagggg ggctgacgta ttgagattag tgcaaagtct cgtgcctggc gtcttcctct
     1981 ggtgcaggat ttgcacccgg ccatcaccgc gccttcaggg catcccggtt gtgccagtcg
     2041 ggaagtcctc acccgcggca agaattgctg ccaggacctc agggtggcct caggatagtc
     2101 acgggccacc catcaccggc ttcttccaaa tcggcacaac gcgcttcaat tcgtcaatta
     2161 gccattggca ggcggcgaag gcttcgcccc gatgcggggc gatgacctcg acccacagcg
     2221 acggctcatt cgccttcacc aggccgatac ggtgaaccag ccgcacggat tcaatcggcc
     2281 agcggcgggc catctcgtcg aacaggaggt tgatctggtg ctccgccatg ctttggaaca
     2341 cttcgtattc gagcgcacta atggtcgcat cgccctcggt cccgcgcacg actcccagga
     2401 agcagaccac cgcgcccatc cccggcgaca tcacccgctg cgagagcagc gcgggttcat
     2461 tgatcggttc ggcggtgatg gtcacctgct tcttcatgcc gcgcccggac tatcctccct
     2521 gcaccggcgg gaacaaggag acctcatccc cctcactcaa gacgtaggtg cgattctggt
     2581 aatcgagtcc gacggccatc aaggtcgctc gctgcatcgc gcctagcttg gggaaacgga
     2641 cgaccagcgt tttaaagagg tcgtcgagcg tgctgccgtc agccagctct tcggtggtgc
     2701 tggcgcagcc ggtcaggtct ttgaaatagg aatagaagca aaccgtcacg cgcatgatgc
     2761 aacccccgaa gccggagccg attgtcaaac agtgcgccgc ccgcccgccc gcttaggctt
     2821 cgcgggaatg acccgctggc aggccttctt gtaaacgtgc ggatgcaaca ccccgacgaa
     2881 cggcaggttg cggtagcgct ccgcgaagtc cagtccgtag cccacgacga agaagtcggg
     2941 gatttcaaac cctacatagt ccgcctcgat ctcttccacg cggcgcgcgg ccttgtttag
     3001 cagaacgcac gtcttgatcc ggcgcggctt gagggcgcgg agcttgccga gcacacggga
     3061 gagcgtcttg cctgtgtcca gaatgtcatc caccagcagc acatcgcgcc cgcgcacgcc
     3121 gagacgcagt tccttggtga agacgaggtc cccggactcg gtgccggcgc cgtaactgga
     3181 gacgccgatg aaatcgagcc gcaagggcag cgagagattg cgtatcagat cggccaggaa
     3241 catcaccgtc ccattgagca gcgagacgac caccatctcg cgcccggtaa aatcgcgctc
     3301 aatgccgcga gacatgctcc ggatgcgctg cgcgatttgg tcatcggtga ttagtatgcg
     3361 ctctacctcc ttgcgccagc gcgtgggaac gcgggcgcgg gacgcgatat taatttcagg
     3421 gacaggtgct ctcatagaac tggggatgtt aaaaggcaac tttggctcgc cgggctggtc
     3481 ttgggtgcag attcggctgc ttcaaatgtg tttggaatcg tgctcgtcct tcacggtgta
     3541 accggactcc tgccgctgga agttcacttt gtgcttttgg atgtaggcat taaacacgtc
     3601 atccgcgctc atgcccagca cctgtgccag gctgatgagg aaatggagca agtccacaac
     3661 ttccacccgg gcgttctgct catcgaactt ctgatacttg gcccaccact tccagggcac
     3721 gctgtcggtc aactcggcga tctcctggga catggcgcgg cagtagttga gcagccactt
     3781 ggtcttatcc tcaacgctca tgccttccat ctggacgccg atgcgctcgt tcaaagcttt
     3841 ctgcatccga aatagctcgt gcaactggtc tggttgttcc atgcgcaaag gattcggctg
     3901 gcttgggcga gaagcaaggc gaaacgacgg caagatgcgc acccttcctc gccccaggca
     3961 actttggcct ttgaacatcg gtgccggggc cgtatataag aagccatgcc aaagttgcca
     4021 gaattgttgt ccaagggcgg ggcgccgctg tggttgctcc tgctcgtcag cgcggtggcc
     4081 gtggcggtgt tcatcgagcg gtttctgcat tgtcaccgcg cccagattaa ttcgaccgag
     4141 ttcctgaacg gcgtgcgcac cgtcctcaag cgcaacaacg tggttgaagc cctgtcaatc
     4201 tgcgacgcta cccccggccc catcgcacgg ctggtcaagg tcgctatcct gaaccgggac
     4261 catggccggg agcgggtgcg cgaagcgctg gaggaagcgg gtgcggcgga ggtgccgcgg
     4321 ctggaggaga agctgaattt gctcgcgacg attgcgcaac tggccccgct gatcggcctg
     4381 ctgggcacgg tgctgggctt catgaagact tttggcgaga tgcaggacga tggcttgaac
     4441 gcgcatatcg gcaagcttgc cgaaggtgtc tggacggccc tggtctgcgc cgcagcggga
     4501 ttggcggtgg cgatcccgac gcacgctggc tacaactacc tggtcagccg catcaattcc
     4561 atcgtgctcg acatggaacg ttccgccact gaaatcgtgc agatcgtcac cgaagacgtt
     4621 aagccccagc cgtaatcatg agattccccc gcaacgccca cattctccgg agccaattgg
     4681 acgcggctcc gttcgcggca gtgttgttcc tgctggtgat cttcatgatg ctggggtcgc
     4741 tggtctatac gccgggggca cggcttgaac tgcagcttcc ccaggccaat ggtttgccgg
     4801 gcaccgacaa gccgacggtc tcggtggcca ttgacgccga cgggcggctc tactacgaaa
     4861 accagtggat cgaggaaagc aacttgcggc gccggttgca agaggccgtc aagaaatcgg
     4921 cggagccgtt ggcgttggtc gtgcaggccg ataaagcggt cagctacgag aagtgcctgc
     4981 ggctcgccct gctggcgcac gatgcgggcc tttcggaagc catattggcc accctgccgc
     5041 gcccctacgc cacaaccccg ccgcgttccc cgcaatgacc tccgagttgg ttgaatcgcg
     5101 gccctggtcg cgcccccact ggtggggcat ggtcgggctc atcttcggcg tccagctcgt
     5161 cttgatcttc tggctgggcg aaacctcgcc gattcgcccg cgcccggctg cgccggctct
     5221 caccctcaaa ctggccggaa gcgcttcggc cgaactgctg gcgctgcatg atcccacctt
     5281 gttcgtcctg ccgcacccgc aggaagttct cgcgccgacg tggctgagaa ctccaccacc
     5341 ggaattccac tccttcgcgt ggcctgagcc gacgaacaac ccgctcctgg ccattgatca
     5401 gcttggcgcc ggcttcaacc ggttggttga aacaacccct ttcaactcac tgccaccgcc
     5461 ggccaaaccc ttgcccgagc tgaccctgcc tgagcttcct ccactggcaa tttccacgcc
     5521 gcactcgacg ttacgcttgg aatgggacca ggcccgtcgg caactcctta cgccgctcga
     5581 actgcgctcg tggcagaacc cggacattct aaccaatagc gtggttcaga tcgttgtgga
     5641 cgctgagggc aggccgggct ccgtcacgtt actctcgggc agtggttcca aggcggcgga
     5701 ccagcaggcc ctggaacaag ccaacgcctc acggttcgag cccctgagcc acaatctcgc
     5761 aggaccagcc ttcaacccgg cggcgcagtt gacttggggc cggatgatct tccagtggca
     5821 cacccttccg gcgcagccca ccaaccctcc ggcggcaggt tcctgaacat gtccatcgcg
     5881 aacttgatat tggcttacgt gatcgtcctg ttggccggca tcaccggtgt gggcatctac
     5941 tccgagctgc gccggcggcg gttcgagccg tctccgagcg aagaccacat cttccgctgc
     6001 cgccgctgcg gctacgtcta taccgacgat ccggacgtgg accgttcacg ctgtacgcaa
     6061 tgcgggcaga tgaacaaggc tattgaattc tgacggagca acggagcaac ctcgctcagc
     6121 ttcccgtctt tagctccatc acatcatgcg gcgccgcttc gcgctggccg gcggggctga
     6181 cacggatgaa acgggcttgc tcgcgaagtt gtgcgaggtt ggccgcgccg agatagccca
     6241 tgccggat
//
LOCUS       DATCGS010000416         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_531438, whole genome shotgun sequence.
ACCESSION   DATCGS010000416 DATCGS010000000
VERSION     DATCGS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531438"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..2001)
                     /locus_tag="VIL39_08550"
     CDS             complement(430..2001)
                     /locus_tag="VIL39_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481514.1"
                     /translation="MQRWPLILFVATTVLAQVITAAAVNAFGEATGVSSQWVYLEASG
                     RLGYRTLTNGDRIMDFSSAGYMGGGVAIPVVPVKVTVSPSGGDDTAAIQEAINRVSER
                     EPVDGFRGAVLLKPGSYDCKGTLTIRASGVVLRGSGSETNGTTLRMGDSPHLCISCSG
                     SSHIERVGRPARMLDGYVPSGTSSFAVGDMSSFKPGDRVLITHPVPAAWIRFMGMDTL
                     VRSGKRETWLSAGSSIETERMIKGVAGNTLTLDIPLTDSFDAKYLNPPGGLVVKCEVS
                     GRISQVGVESLRIFAPAQVVEITQRHHEAIQMNGVSDAWLRDIAIEDTVNSVGLGSSV
                     NRITVERVSVRHSLATKGAAKPADFSVNGAQVLFNRCSSTGNNLFYFVTGARVTGPNV
                     LLNCIFHGNGHIQPHARWATGLLVDGCQVPESGIEFMNQGEMGSGHGWTIGWAVAWNC
                     VARSYIIQQPPGAVNWAIGCRGRRDESGMPFGHKPNLPEGIFDSHGTPVTPASLYLAQ
                     LRERLGPQALKNIGY"
     gene            complement(1983..2498)
                     /locus_tag="VIL39_08555"
     CDS             complement(1983..2498)
                     /locus_tag="VIL39_08555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5481515.1"
                     /translation="MTGLSDVLQLRVRYSETDQMGTFYNSRALEWFECGRTELMRRRL
                     GMSYTALEARGVFLPLVEAHLEFQGRARYDDLLEIASTVNVSGRAKLRFDVQIHQGES
                     QKPVVCGYTVHAFTDRQGKPIRPPAWFVAALQEPSSPVVPIGSSHGDTPGVLRQSALP
                     AVHENPCSVGH"
     gene            2880..4331
                     /locus_tag="VIL39_08560"
     CDS             2880..4331
                     /locus_tag="VIL39_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEY5481516.1"
                     /translation="MTTKAQALTLITLGATLMICPAQTTQTTASAPASSPPALSSTEQ
                     TIKDIKNPAPWLNWGADFRARNEYFNDLLTLNPNAALHEQDYFRFRARLWVSVTPLEN
                     LSVNARIADEVREWMLPAGYTPYKGSSGLDWREGIIDSLNVDWKNAAGLPLTIKVGRQ
                     DLFLGDGFLVGDGTPIDGSWTYYLDSARLIYDLKDQHTTIDAIGIIQNAHDDAWLPTI
                     NPNHLLVTEQNEKGGILQVANSSIKAANLTGYFIYKNDTRVAANGDNADIYTLGGRIN
                     GPLSDHWTYWVEGAYQLGRKQDLSLKHSDAGAAAALDYNNLNAFGFNSKLTYLFKDKL
                     NNQLTMSLELLSGDNPSTGNDEMFDVLWGRWPRWSEIGLYSYAAETRIGQEANLIRFG
                     PTWTINPIKDLEFSASYYALFAMTDVATRAASTTLFSGNDNFRGNFASAMLKYKFSPH
                     MNGHLWGEMLFPGAYYANTTLIPFLRADVMVTF"
     gene            complement(4334..4531)
                     /locus_tag="VIL39_08565"
                     /pseudo
     CDS             complement(4334..4531)
                     /locus_tag="VIL39_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017930245.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
BASE COUNT          977 a         1461 c         1308 g          897 t
ORIGIN      
        1 tgtgggggtg tttcggcccg tcagccgtcg ccagcgcctg tgacggcgtg ggcgtgcaga
       61 ccaactacgc tcgttacatt cactacaagc agtcgctcaa cgccaaggaa gccgtggccg
      121 gattcctttg ggcggcggag atggtcgaac ggccacagtt ggcgagaatc aagaagtgat
      181 caggctgttc acttacccgt atcaagctgc tccacgacct ttgggcgaag caagttgcta
      241 cggttggatg gttccacggg cggcaatggc ttcacccaat cgcgatactt gcggtcggga
      301 agtttcaggg cggcttggcg caacagcggg aagagcatct cgggcggcca cccgccgatt
      361 tggcggtgcg gccacttctg ttgtcccaaa gcaaaaggcg caagataatc gagcgccttg
      421 gcaagggttc taatacccga tattcttcag ggcctgggga cccaaacgct cgcgcagttg
      481 cgccaggtaa aggctggcgg gggtcacggg agttccatga gaatcgaaga tgccctcggg
      541 caggtttggc ttatggccga aaggcatgcc gctttcatcg cgcctccctc tgcagccgat
      601 agcccagttc actgctccgg gcggctgttg gatgatgtaa ctccgggcaa cacaattcca
      661 cgccacggcc cagccaatcg tccagccatg gccggagccc atctcgccct ggttcatgaa
      721 ttcaatgccg ctctccggca cctggcagcc gtccaccaac aaacccgttg cccaccgggc
      781 atgcggctgg atatggccat tgccatggaa gatgcagttc aagagcacgt tcggaccggt
      841 caccctcgcg ccggtaacga agtagaatag gttgttcccc gtcgaagaac agcggttgaa
      901 caacacctgc gcgccgttca cagagaaatc cgccggcttg gctgcgcctt tcgtcgccaa
      961 gctgtgccgg acactcaccc gctccactgt tatccggttg acgctgctgc cgaggccgac
     1021 gctgttcacc gtatcctcga tcgcaatgtc tctgagccag gcatcgctca cgccattcat
     1081 ctggatcgcc tcatgatgtc tctgggtaat ttccaccacc tgggccggcg cgaagattcg
     1141 caagctttcc actccgacct gcgaaatgcg tccggagact tcgcacttca ccaccaagcc
     1201 gcccggggga ttcagatact tggcgtcgaa tgaatccgtg agcgggatgt ctaaagtcag
     1261 ggtgttgccc gcgacgcctt tgatcatccg ttcggtctct atgctactgc ccgccgaaag
     1321 ccaggtttcg cgcttgccgc tgcggaccaa agtgtccatg cccataaacc tgatccaggc
     1381 tgcggggacg gggtgggtga tcaggaccct gtctccgggt ttgaaactcg acatgtcccc
     1441 gacggcaaac gaactcgttc ccgacggcac atagccgtcc agcatgcgcg ccggcctgcc
     1501 aacccgctcg atatggctcg aacccgagca ggagatgcag agatgcgggc tgtcgcccat
     1561 ccgcagtgtc gtgccatttg tctcggagcc gctgccgcgc aacaccacgc cactggcgcg
     1621 gatggtcagc gtccccttgc agtcataact gcccggcttc agcaggaccg ccccgcgaaa
     1681 cccgtccacc ggttcccgct ccgacacccg gttgatggct tcctggatcg ccgccgtgtc
     1741 gtcaccgccg gatgggctga cggtcacttt gactggtacc acgggaatcg ccaccccgcc
     1801 gcccatgtag ccggcggaag agaagtccat aatgcggtcg ccgttcgtca gcgtccgata
     1861 gccgagccgg ccactcgcct cgagataaac ccactggctg ctaacccctg ttgcctcgcc
     1921 aaaggcattc accgccgcag ccgttatgac ctgggcgaga acggtggtcg cgacgaatag
     1981 gatcaatggc caacgctgca tggattttcg tgaactgctg gaagcgccga ctgcctcagc
     2041 acacccgggg tgtcgccgtg ggaggagccg atagggacga ctggcgaaga aggttcctgc
     2101 aacgcagcca cgaaccaggc cggcggacga atcggcttgc cctgtcggtc cgtgaaagca
     2161 tgaaccgtgt agccgcacac caccggcttt tggctctcgc cctgatggat ctgcacgtcg
     2221 aaccgcagct ttgcccggcc tgatacgttc accgtggaag caatctcaag caaatcgtcg
     2281 tagcgcgctc tcccctggaa ttccaagtgc gcttcaacca gcggcaggaa aacgccccgc
     2341 gcctccagcg cggtataaga catccccagc ctgcggcgca tcagttccgt ccgcccgcac
     2401 tcaaaccatt ccagggcgcg cgaattatag aacgtcccca tttggtccgt ctcgctatag
     2461 cggacgcgca actgcaacac atcggataac ccggtcatca gggcagcatt gccgcgcgga
     2521 tcgacaagtt caactaaatc ctaaaggctg cggctttgct cgacaactgc gcccgggtcg
     2581 cgctttctac gcatgctact ggagcggatc tcggacaatc tgcgcagtcc cgactttccg
     2641 tatcttgcgc ctctgactgc aattaatgca aataacgggg aagaataatc ttcatggcag
     2701 tttatcaata acactggaca ctcggattcc ggcggctagt attgcggcgg cctcactgga
     2761 aaagagactt tgaggggcgg ccgaaagaag gccgtcaata tgagcgagaa ccttaacctg
     2821 aaaacatcga ctgatgtgct cattcacagg agtagcaccg gtcttaaatg caacaacccg
     2881 tgacaactaa agcccaagca ctaactctca tcacgctggg ggcaaccctg atgatctgcc
     2941 cggcccaaac cacccaaacc actgcgtcag caccagcttc gtcgccgccc gctctcagct
     3001 cgacggaaca gacgattaaa gatatcaaga atccggctcc ctggctcaat tggggtgccg
     3061 acttccgggc acgcaacgaa tacttcaacg acctgctgac gctaaacccg aatgcggcgc
     3121 tgcacgagca ggactacttc cgcttccgtg cgcgtctctg ggtcagcgtc acgccgcttg
     3181 aaaacctcag cgtgaatgcg cgaatcgccg atgaagtccg ggagtggatg ctgccggcgg
     3241 gctacacccc gtacaaaggc agctccggcc tggactggag agagggcatc atcgacagcc
     3301 tcaatgtgga ttggaaaaat gcggcaggct tgcccctgac catcaaggtg ggtcgccagg
     3361 acctgtttct gggtgatggc ttcctggtcg gggatggcac gcccatcgat ggctcctgga
     3421 cgtattacct ggactcggcc cgccttatct atgatctcaa agaccagcac acgacgattg
     3481 acgcgatcgg gatcattcaa aacgcccacg atgatgcctg gctcccgacg atcaacccca
     3541 atcacctgct cgtgaccgaa caaaacgaaa agggcggcat cctgcaagtt gccaacagct
     3601 cgataaaggc tgccaatctg accggctact tcatctacaa gaacgacact cgggtcgcgg
     3661 ccaacggcga caatgctgat atctacactc tgggcggccg gatcaatggt ccgctaagcg
     3721 accactggac atactgggtc gaaggcgcat accagttggg ccggaagcag gatctaagct
     3781 tgaagcattc ggatgccggg gctgccgcag ccttggacta taataacctc aacgcctttg
     3841 gtttcaacag caagctcacc tatctgttca aggacaagct caacaaccag cttaccatgt
     3901 cattggaact gctctccggc gacaatccga gcaccggcaa cgatgaaatg ttcgacgtcc
     3961 tctggggccg ctggccgcgc tggagtgaaa tcggccttta cagctatgcg gcggagaccc
     4021 ggatcggcca ggaggccaac ctgattcgtt tcggcccgac ctggaccatc aatcccatca
     4081 aggacctgga gttcagcgcc agctactacg ccctgtttgc tatgaccgat gtggcaactc
     4141 gcgcagccag cacaacgttg ttcagtggca acgacaactt ccgtggcaat ttcgcctcgg
     4201 cgatgctgaa gtataaattc agcccgcaca tgaacggcca tctttggggc gaaatgctat
     4261 tcccgggcgc ttactacgcc aacaccacgc tgattccttt cctgcgggct gacgtgatgg
     4321 tcaccttcta gccgggaatg atgttgcgcc tggtgccgcc gtggatggag ccgaccgtga
     4381 ccacggccga ttcgccgggc ttgacctcac ggctgacgat ggtctgcagg gcgaggatga
     4441 tctgtgccgc caggaccacc gggtccttgg tcttgtgcgg cgctacgccg tgcccgccca
     4501 cgccctgaac gatgatgtcc acggaatcga ttgttggccc cagaatagcc gggcgtgcca
     4561 gatcatgtgg aagtcgtggc cgcaggcatg catgacgggc acgtccgtat tgcctggcag
     4621 actccgtcat cgccgttcac cgt
//
LOCUS       DATCGS010000417         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_85126, whole genome shotgun sequence.
ACCESSION   DATCGS010000417 DATCGS010000000
VERSION     DATCGS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85126"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..711)
                     /locus_tag="VIL39_08570"
     CDS             complement(205..711)
                     /locus_tag="VIL39_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY5481517.1"
                     /translation="MYKIIGADGREYGPITADQLREWIAEGRASAQTWALAEGAAQWK
                     PLTEYLEFAPALGRTPPPLSAPGPISITRAPRTNSLALMGMLMGVLSITCGMCCCYGM
                     PFNLLGIVFSLIALAQIRNDPHSQEGRGLAIAGLVLSLLSLLLAVLFFAFGLALSTPD
                     ILRKIQRL"
     gene            complement(738..1010)
                     /locus_tag="VIL39_08575"
     CDS             complement(738..1010)
                     /locus_tag="VIL39_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEY5481518.1"
                     /translation="MTPPPCAPAPRAANKLPAGICGILLGSLGVHKFILGYTGAGLVM
                     LLVTLLTCGIGGCVMHVIGLIEGIIYLCKPDEEFVRVYVDGRKEWF"
     gene            complement(1071..1214)
                     /locus_tag="VIL39_08580"
                     /pseudo
     CDS             complement(1071..1214)
                     /locus_tag="VIL39_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
     gene            complement(1263..2120)
                     /locus_tag="VIL39_08585"
     CDS             complement(1263..2120)
                     /locus_tag="VIL39_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY5481519.1"
                     /translation="MYRILGADGKEYGPVNGELLRQWITEGRANAHTKVKPEGSADWQ
                     TLASVPEFEPAFAASPGAPSPLPTTPVEVKTSGMAITSLVLGVLGFCGITAVVGLILG
                     IVALVKINRSGGRLSGQGLAIAGICVSAFMLLFSIPIMAGMMLPALSQAKQKAQAINC
                     VNNLKQLGLAVRIYANDHNDQFPPAATWCDAIQGSVGSPKVFQCPSEPGRRCAYAFNA
                     KLDGKKVGEIDPQTVLLYESDAGWNGTGGAENLKPHKHSSRTVNVAFADGSVQQLPRS
                     RLGTLRWDP"
     gene            complement(2182..3231)
                     /gene="trpD"
                     /locus_tag="VIL39_08590"
     CDS             complement(2182..3231)
                     /gene="trpD"
                     /locus_tag="VIL39_08590"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5481520.1"
                     /translation="MLKEFTRQLAASNALTDGQVRLAVELLVDEAVPAAIKAEFLTHL
                     ARKGETTEEIAAFARELRARSIQPTLDAELRSAEILDVVGTGGDRLSTFNISTTVALI
                     AAAAGVRVAKHGNRASTSPTGSADVMEALGITFDLGPEEAVRSLREHSFAFFFAPKYH
                     PAFRHIIPARKLCAERGQSTIFNFLGPLLNPAQPSAALIGVPRPDLCEPLARVLQTLG
                     MRRAMVVCGAVPGEGGAVRYLDELSPLGPTTIAEFYQERALVSSVLAPESFPLQPAAL
                     ADLRGGDRLVNAVIVRRVLHGEERGPKRDAVLLNAAAALFVAGRTRTMAEGWDLAAET
                     VDSGKARAKLAELQG"
BASE COUNT          649 a         1071 c         1096 g          592 t
ORIGIN      
        1 cccccggagt gcgcccgtcc tcgggcgcag cgatgtccgc gagcagtggg gtttcgcagc
       61 tttccagcgt cttgggcgag tcagaacctg ctgcgcccga ggacgggcgc actccggcgc
      121 agtcgccttc ggtggtggta accgcaagag gcacccccac tgaggggcga gcgattttga
      181 accggcttac ggagaacgac atgcctatag tctctgtatt ttgcgcagga tgtccggcgt
      241 gctcagcgcg aggccgaacg cgaagaacag caccgccagg agaaggctca acagggaaag
      301 caccagcccc gcgatggcca ggccccggcc ctcttgcgaa tgcgggtcgt tcctgatctg
      361 ggcgagggcg atgagggaga agacgatgcc cagcagattg aacggcatgc cgtaacagca
      421 gcacatgccg caagtaatcg agagaacgcc catgagcatg cccatcaacg ccaatgaatt
      481 cgtccgggga gcccgggtga tggagatcgg ccccggagcg gacaagggcg gaggtgtgcg
      541 gccaagcgcc ggggcgaact cgagatactc cgtgagcggc ttccactggg ccgcgccttc
      601 agccagcgcc caggtttgcg cgctggcccg gccttcggca atccactcgc gcaactggtc
      661 cgccgtgatc ggaccatact ccctgccgtc tgcgccgata atcttataca tgacctgatt
      721 ctacccggga aaggcggcta aaaccactct ttccgcccgt caacatacac ccgcacgaac
      781 tcctcgtcgg gcttgcacag gtagataatg ccctcgatca agccgatgac gtgcatcacg
      841 catccgccaa taccgcacgt caggagggtg acgagcagca tgaccaggcc ggcgccggta
      901 tagccgagaa tgaatttatg gacgccgagt gagccaagca ggatgccgca aatgccggca
      961 ggaagtttgt tggccgcccg aggcgcaggg gcgcagggcg gcggcgtgat cgtcggcggc
     1021 acagccgcgg agaccggcgg gggcttcagg tcggcagcga actcaggcag agaaccgagc
     1081 ggtttccagt ccggggtgcc gtcggcttgc acgagggttt gggcattggc gcggttttcg
     1141 gccatccaac tccgcagttg ctccgcagta accgggccat attgctggcc gtcttttcct
     1201 acaattctat acatagtgtt tctttcgtac cctatactta cgaaaaagcc ggtcgaggtt
     1261 acttagggat cccagcgcag ggttccgagc cgcgaacggg ggagttgttg cactgaaccg
     1321 tccgcaaagg ccacgttcac ggtgcggctg gagtgcttgt gtggcttaag gttttcggcg
     1381 cccccggtac cgttccaacc ggcgtcactc tcatacagca gcacggtttg ggggtcaatc
     1441 tcccccacct tcttgccgtc cagcttcgca ttgaacgcat acgcgcagcg gcgaccaggc
     1501 tcggaggggc attgaaacac tttaggcgag ccgacactgc cttggatcgc atcacaccag
     1561 gttgccgccg gtggaaactg gtcgttgtga tcgttggcgt agattctcac tgccaggccc
     1621 agttgcttca ggttgttgac gcaattgatg gcctgcgcct tttgttttgc ctgggacaac
     1681 gccggcagca tcatcccggc cattatgggg atgctgaaca acagcatgaa ggccgacacg
     1741 cagattccgg caatcgccaa tccctggccc gacaatcgcc cgccgctccg gttgatcttg
     1801 acgagcgcga cgatcccgag aatcaaaccc accaccgccg tgatgccgca gaatcccagg
     1861 acgcccagca ccagcgaggt gatcgccata ccactggtct tgacttccac tggggtcgtg
     1921 ggcaggggag atggcgcgcc gggggaggcg gcaaaagccg gctcaaattc cggcaccgaa
     1981 gcgagcgtct gccaatcggc gctgccttcc ggtttgactt tggtgtgcgc gttggcgcgg
     2041 ccttcggtga tccactggcg caacaattcg ccgttaaccg ggccatattc tttgccatca
     2101 gctccaagga tgcgatacat atagtctttc tacgctgcct cgcgcctccc gccaagttga
     2161 aaaagtgccg gattttgtaa tctacccctg taactcagcc agcttcgcgc gggccttgcc
     2221 gctgtcaaca gtttcagccg ccaggtccca gccctcggcc atggtccgtg tgcgaccggc
     2281 gacaaacaac gcggcggcgg cgttgagcag aacggcgtcg cgcttcgggc cgcgctcctc
     2341 accgtgcagg acgcggcgga cgatcacggc gttgaccagg cggtcaccgc cgcgcagatc
     2401 ggccagtgcc gccggctgca gcgggaaaga ctccggcgcc agcacggaag agaccagcgc
     2461 gcgctcctgg taaaactcgg caatcgtggt cggcccaaga ggtgaaagct catcgagata
     2521 gcggaccgcc ccgccctcgc ccggcaccgc gccgcagacg accatggcgc gccgcatgcc
     2581 gagggtctgg aggacgcggg ctagcggctc gcacaggtcc gggcgcggca cgcctatcag
     2641 tgccgcactg ggctgcgccg gattcaacag cgggccgagg aagttgaaga tcgtgctctg
     2701 gccgcgctca gcgcagagct tgcgcgcggg gatgatgtgc cggaaggcgg gatggtattt
     2761 cggcgcaaag aagaaggcga agctatgctc gcgcagagac cgcacggctt cctccggtcc
     2821 caggtcaaag gtaatgccca gcgcctccat cacgtcggcg ctgccggtcg gcgaggtgct
     2881 ggcgcggttg ccgtgcttgg ccacccgcac gccagccgcc gcggcaataa gcgccacggt
     2941 ggtggagatg ttaaacgtgc tcaggcgatc gcccccggtg cccacgacgt ccaggatttc
     3001 agcggaccgc aactcggcat ccagcgtcgg ttggatggac cgggcgcgca gctcacgggc
     3061 gaacgcggca atttcctcgg tcgtctcacc cttccgggcc aggtgggtca ggaactcggc
     3121 tttgattgcg gccggcacgg cctcgtccac cagcaactcg accgcgaggc ggacctgccc
     3181 gtccgtgagg gcgttcgatg cggcgagctg ccgcgtgaat tctttaagca cgagcgaata
     3241 gtaagagccg gcccggccac tttgcaagaa agcgtcggcc ctggcgacag ggctggcatc
     3301 aagctcgcgg aaaccattcg tacatagcag ggcggtcagt cctgaatggc gcttagttaa
     3361 ggccccagca acagcggggg cttgacaacg gttcttaggg ctaaggtg
//
LOCUS       DATCGS010000418         2487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_481936, whole genome shotgun sequence.
ACCESSION   DATCGS010000418 DATCGS010000000
VERSION     DATCGS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481936"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1371
                     /locus_tag="VIL39_08595"
     CDS             298..1371
                     /locus_tag="VIL39_08595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5481521.1"
                     /translation="MNHRLNLPRPIQILSVLAVAALFAGCKTGPRPGTFAPRKGDEIV
                     VAGQFVHTGTRVVLWMDPGGYDAYRVERRFSPIEKSDWETSQAEVRGLSSPNRYNMRN
                     RDLSREEVERVRGGGWDLPLLQRVVDQFVLHFDVCGTSRQCFKVLQDGRALSVHFMLD
                     LDGTIYQTLDLKERAWHATSSNSRSVGIEIANMGAYPVNGRNPFADWYAKDSSGRTRI
                     TIPAQYGDGGIRTKDFVGHPARPEPVTGVLQGKELIQYDFTPQQYQALIKLTATLSKA
                     FPQIKCDYPKDATGQLIRQKLPDAELKSYQGVLGHFHIQTDKVDPGPAFQWDYVIGGA
                     RKLLHGGLSGMADETSKGHMRAR"
     gene            1368..>2487
                     /locus_tag="VIL39_08600"
     CDS             1368..>2487
                     /locus_tag="VIL39_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5481522.1"
                     /translation="MKKLFLLDGMALVYRAHFAFISRPILTSKGLNTSALYGFTQTLL
                     DIQKNQQPTHLGVAFDTQAPTQRHIEFPDYKANRQEMPEDLSAALPHVRRMVEALNIP
                     VLICDGYEADDIIGTLVRCAGKEGFTSYMVTPDKDFGQLITENTFIYKPSRSGEGVEI
                     IGLPEIQSRWGVQRPEQVVDVLALMGDASDNIPGVPGIGEKTAMKLIAQYDTVENLLA
                     HAGELTGRLKQNLETNRDLALLSKRLATIICDAPCAVELDTLKVQPPDEEKLKGLLVE
                     FEFNSIGRRLFGEDFKAGHGGGSPKSEVQSPKSQVPSPKSTPAAQGDREAEQFVLVSE
                     MEGGAVEEKPKPAVVASNLKTIADVPHEYHLVATPEERA"
BASE COUNT          509 a          750 c          769 g          459 t
ORIGIN      
        1 cctggaacat tcggagtcgg cgttgctgga ccgtgccgcc gcatgagaat cctgttggat
       61 gaatgcgttc cgtggccgac gcacagactc ctgtgccgga catgagcttt ccaccaacaa
      121 acttcgccgc ctctctgcag cggcggcctc gatccaatcc gccatcgcca ccatgaaacc
      181 gggtgagttc cgccacctgg aaatacctta agccgggctg gcgatcctgc ccctgcctga
      241 gcttgactgg ccgccattca aatggcactt agtctggtga ttacaacgta acaccttatg
      301 aaccatcgcc taaatctccc tcgcccgatc caaattctca gcgtgctcgc cgtggcggcc
      361 ctatttgccg gctgcaagac tgggccccgg cccggcacct ttgcgccccg gaaaggggat
      421 gaaatcgtcg tcgctggcca gttcgtgcac accggcacgc gcgtggtgct ctggatggac
      481 ccgggcggct acgacgcgta tcgcgtggaa cggcgcttca gtcccattga gaagtctgat
      541 tgggaaacct cgcaggcgga agttagaggc ttatcttcgc ccaatcgcta caacatgcgc
      601 aatcgcgacc tcagccgcga ggaggtcgag cgggtgcggg gcggcggctg ggatttgccg
      661 ctcctgcaac gggtggtgga tcagtttgtc ctccacttcg acgtgtgcgg cacgagccgg
      721 caatgtttca aggtgctcca ggacggccgc gctttgagcg tgcatttcat gctcgacctg
      781 gatggcacga tctaccagac gctcgacctc aaagagcgcg cgtggcacgc aacctcctcg
      841 aacagccgct ccgtgggcat cgaaatcgcc aacatgggcg cctatccggt caatgggagg
      901 aatccttttg cggactggta tgccaaagac tccagcggcc ggacccgcat caccatcccc
      961 gcccagtatg gcgatggcgg cattcgcacc aaagacttcg tcggccatcc ggcgcgcccg
     1021 gaaccggtca ctggggtgct ccaaggcaag gaattgattc aatacgactt cacgccgcag
     1081 caataccagg ctctgatcaa gcttacggcg acgctcagca aggcgttccc gcaaatcaaa
     1141 tgcgattacc ccaaggatgc caccggccaa ctcatccgcc aaaagttgcc ggacgccgaa
     1201 ctcaagagtt accagggcgt gctgggccat tttcacatcc agacggacaa ggtcgatccc
     1261 ggcccggcct tccaatggga ttatgtgatc ggtggcgccc gcaagctcct ccacggtggc
     1321 ctttctggaa tggcggacga gaccagcaag gggcatatga gggcaaggtg aagaaactct
     1381 ttctgctgga cgggatggcg ctggtgtatc gggcgcactt tgcctttatc agccgcccaa
     1441 ttctcacgtc gaaagggctc aacacctccg cgctctacgg ctttacccag acgctgctcg
     1501 acattcagaa gaatcagcag ccaacgcacc tgggcgtcgc cttcgacacc caggcgccga
     1561 cgcagcggca catcgagttt cctgactaca aggcgaaccg gcaggagatg cctgaggacc
     1621 tgagcgcggc cttgccccac gtccgccgga tggtcgaggc gctcaatatc ccagtgctca
     1681 tctgcgacgg ttatgaggcc gacgacatca tcggcacgct ggtgcggtgc gccggaaaag
     1741 aaggtttcac gtcttacatg gtcacgcccg acaaggactt cggccagctc atcaccgaga
     1801 acacctttat ctataagccc tcccggagcg gggagggggt cgagatcatc ggcctgccgg
     1861 agattcagag ccgctggggt gttcaacgcc cggaacaggt ggtggatgtg ctggcgctga
     1921 tgggcgatgc gtcggataac atccccggcg tgcctggcat cggggagaag acggcgatga
     1981 agcttattgc gcagtatgac acggtggaaa atctgctggc gcacgcgggt gagttgaccg
     2041 ggcggctcaa acagaacctg gagaccaatc gcgacttggc gctgctgtcc aaacggctgg
     2101 caaccatcat ttgcgacgcg ccctgtgcgg ttgagctgga tacgctgaag gtgcagccgc
     2161 ccgacgagga gaagctgaag ggtttgctgg tggagttcga gttcaactcc attggccggc
     2221 gcctgttcgg cgaagacttt aaggcaggtc acgggggggg aagtcctaag tccgaagtcc
     2281 aaagtccgaa gtcccaagtc ccaagtccca agtccactcc agccgcgcag ggtgataggg
     2341 aggccgagca gttcgtgctg gtttcggaga tggaaggggg ggcggtggag gagaagccca
     2401 aaccggctgt ggtggcgtcg aacctgaaga ccattgcgga cgtgccgcac gagtatcatt
     2461 tggtggccac gcctgaggag cgggcgg
//
LOCUS       DATCGS010000419         8887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_580275, whole genome shotgun sequence.
ACCESSION   DATCGS010000419 DATCGS010000000
VERSION     DATCGS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580275"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIL39_08605"
     CDS             complement(<1..432)
                     /locus_tag="VIL39_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5481523.1"
                     /translation="MLEIQNLTAGVEGKQILKGINLTIYPGEVHAIMGPNGSGKSTLA
                     AILAGREGYEVTAGRVVYDGKDLLELDPEERAREGLFLAFQYPVEIPGVNSTYFLKSA
                     LNEIRKHKGQTELDAMEFLTLVKDKIQLLELKEDLLRRSVNE"
     gene            complement(590..2038)
                     /gene="sufB"
                     /locus_tag="VIL39_08610"
     CDS             complement(590..2038)
                     /gene="sufB"
                     /locus_tag="VIL39_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY5481524.1"
                     /translation="MSTATETIENLVGKEYKYGFYTDVEADSAPPGLNEDIVRFISAK
                     KKEPEFMLDWRLKAYRHWLTLKEPTWPQVHYPPIDYQASVYYSAPKKKGDGPRSLEDV
                     DPKLLETYEKLGIPLHERGRLAGVAVDAVFDSVSVGTTFKKQLAEKGIIFCSFSEAVQ
                     EHPDLVKKYLGMVVPYTDNYFAALNSAVFSDGSFCFVPKGVRCPMELSTYFRINAANT
                     GQFERTLIVAEEGASVSYLEGCTAPMRDEHQLHAAVVELIALDNAQIKYSTVQNWYPG
                     DAQGRGGIYNFVTKRGLCKGKHSKISWTQVETGSAITWKYPSCILLGDNAVGEFYSVA
                     VTNNYQQADTGTKMVHIGKNTRSTIVSKGISAGHGQNSYRGLVKIQKGAAHARNFSQC
                     DSLLLGDKCGAHTFPYIEVKNTTARVEHEASTSKIGEDQIFYCNQRGLATQDAVNMIV
                     NGFCKEVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(2046..2525)
                     /locus_tag="VIL39_08615"
     CDS             complement(2046..2525)
                     /locus_tag="VIL39_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5481525.1"
                     /translation="MVKTVPEETDNRLNERLATRGFRFTPQREHVYAVLLRKRDHPTA
                     EEVFIRAKREIPDISMATVYNCLDALVECGLARQVTLERGATRFCPNMREHCHFYCDQ
                     CEKVFDIELPAETGIALPKGYRAEWYDIAIHGRCPACKGGRREKDLASGMSRMGYIG"
     gene            complement(2577..2882)
                     /locus_tag="VIL39_08620"
     CDS             complement(2577..2882)
                     /locus_tag="VIL39_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481526.1"
                     /translation="MREVLGERAALVVRPKAPAPGSPWIWRTEFFGHEPQADIALLGR
                     GFQVAYVDMQNLYGAPVAVQHMERGCAIRGCKAARRTSPALEIMGQWAAWAGIQPKV"
     gene            complement(3071..4447)
                     /locus_tag="VIL39_08625"
     CDS             complement(3071..4447)
                     /locus_tag="VIL39_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481527.1"
                     /translation="MKTTTKSLCVSMLCGCLFGLTANAQMMLENFEEYPSDADLQAVW
                     SPDMSATLTLSSYVAYRSTGTNSMRVDISVPANAWQTTVLNTQPLPAPIAISPTQYIT
                     LRIAGDPQYTNSTFNMIYVYAWDGNGNFGRWGSAVPTTTTNWQVFNFPASSVEVPWNS
                     PGAPDLTNIAQFRFYLYGQNGAPDPANAFTATIYLDELQVRNSPLIEFPPPSPMRAKI
                     DNFEEYADATALLGFYHYVNGAPTTVTTASLETPAPQGTNALKLAIDFGAGQWPWGAV
                     YSAVVAPFSFPTNAVVSLWLKGDPTLAPVADSGTVFYLSFYDQGGNALNFTTPAAPVI
                     SSGWTRLQANFNQFWSGAVVDTGNLVQWRVLVEGWMGTADSTALSASFAVDDIQITVP
                     PVLTVVRNGSALTLQMDSLLPGTTYTLRQTSDFSQWTTTTIQATSTSQTWPIPAGQKG
                     FYQLFYTP"
     gene            4861..7668
                     /locus_tag="VIL39_08630"
     CDS             4861..7668
                     /locus_tag="VIL39_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY5481528.1"
                     /translation="MATVWTVALFLFGRGHSLGAPLIIDDFAYTNSAAARQAWVGVSA
                     PAVNMAASGDWGTDQVMTLTCDFATRDVRCYWDRTVSLNLAAFTDFVLEVFAPDPGAI
                     SSFTLYFHSGAGWYGGSASLSQTGWQTLRFSKASFIPEGTPSGWDQIDGIRLSPWKGA
                     ARNTYLAARQLRAFTPPVLLVRDDDSSNPTVVQQTIALHTQWLGGYNIDCGVLARTNV
                     EAGLLAQSRLVILPYNENISAAEWTALESFVAAGGKLLVYYLLPARMEALLGVRATGW
                     AAGDFAAWVFADPAIPGLPARVQQASWNITYAVTNGLNSRVSATWVDSHGASTGHAAW
                     LTSDHGFFMSHILLDDDPDQKAYALLCLVGYFLPDVWPAAAVGAIDGIGRVGPYNTYA
                     EAVAGIRHDAESTLRAPLAEAELATTAAERDRALAGVAATNYPQAILAAQSAQDHLRQ
                     AYFLSLKPVTPELRAFWEHHATGPYPGNWAAAADALVTNGFTAVFPNMLWGGLAHYNS
                     AFLPHSTDFTNYGDQITACVSAAHARGLQVHVWKVNWNLSGAPQSFIDSLRAANRTQV
                     SSSGVAIDWLCPSHPDNFALETNSMLEVVRNYDVDGIHFDYIRYPDSDYCYCTGCAAR
                     FQSQTGQAVTNWPAGVLATGALRNSFLDWRRAQITRLVTAVHAGVKAIKPKVQVSAAV
                     FPDAASAFDGVGQDWRAWITNGIVDFLCPMDYTTSLSQFTNLVAGQLTYAAGRVPIYP
                     GIGAYVLQPDAALAQLQQTRAANTHGFIIFELSPEAVTNLLPAIRAGATAPDEPDTDN
                     DLLPDSWELRWFTNLSTAGLNTDTDGDRLTDRDEYVLGTNPTQPNPGLTLQARLVGGT
                     VEVSFLAQAVAGAGYQNAERHYCLESRTTLAGGSVWEPVAGFADRTIASGSETLVFSV
                     SPAPTQSALFRARVWLQQKQR"
     gene            7824..8690
                     /locus_tag="VIL39_08635"
     CDS             7824..8690
                     /locus_tag="VIL39_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY5481529.1"
                     /translation="MKVDRASWLSRRSRKVALAACLLQLTGFGSFAAAGAEPTNLDSD
                     PISLLLAATFKITNKQSSGTCFLIEPPPPSGWSNGVVILVTAAHALEEAPGTECRIVM
                     REKDAASSYLRKEVPLTIRSGEKALWVKHPHEDVAAIKFTLPLGVSCQPLRLDQLARA
                     DDFSNGKIRLASDTWIFCFPAHLEANDAGLPVLRHGSIASLPLIPLSSNRTFLVDFNT
                     FGGDSGAPVMMGARGAIQPQALIVGLVLGMQRQTDKASMPFEERTVHHPLALAIVAHS
                     EIIRQTVELVLR"
BASE COUNT         1687 a         2895 c         2493 g         1812 t
ORIGIN      
        1 ctcgttgacc gagcgccgca gcaaatcttc tttcagttcc agcagttgga tcttgtcctt
       61 caccagagtc aggaactcca tcgcatccag ctccgtctgg cccttgtgct tgcggatttc
      121 gttcagcgcc gacttgagga aatacgtgct gttcacgccg ggaatctcga cgggatattg
      181 gaacgcgagg aagagtccct cgcgggcccg ctcttccggg tccaactcga gcaaatcctt
      241 gccgtcatag accactcggc cagccgtcac ctcgtagccc tcgcggccag ccaggatcgc
      301 cgccagcgtg ctcttgccgc tcccgttcgg tcccatgatg gcgtggactt cgccgggata
      361 aatcgtcagg ttgatgccct tgagaatctg tttgccctcc accccggcag tgaggttctg
      421 aatttccagt atcgcttgtt tcattgtcgt ctgatctgac ttcctgaact ccgattcact
      481 atgcctgccg gttgccaatg caacccatgc aacctatgta actcattcca tgtaactcca
      541 ccaaggccgc taacttccgt aacaggtttg acctgctacc ggccgcgcct catcccacgc
      601 tgccttccag gctcacgccc aacagcttct gcgcctcgac ggcaaattcc atcggcagtt
      661 cccggaacac ctccttgcag aagccgttca caatcatgtt cacagcatcc tgcgttgcca
      721 gtccccgttg gttgcaatag aagatctggt cctccccgat cttcgaagtc gaggcctcat
      781 gttccacccg cgccgtcgtg ttcttcacct cgatgtaggg gaacgtgtgc gccccgcatt
      841 tgtcgcccag caataatgaa tcgcactggg agaaattccg cgcgtgcgcc gcccccttct
      901 ggatcttcac caggcctcga tagctgttct ggccatgccc ggccgaaatg cccttggaca
      961 caatcgtgct gcgcgtgttc ttgccgatgt gaaccatctt ggtgcccgtg tccgcctgtt
     1021 gataattgtt cgtcacggcc accgagtaga actccccgac cgcattatcg cccagtagaa
     1081 tgcagctcgg gtatttccac gtgatcgccg agccggtctc cacctgcgtc cacgaaatct
     1141 tcgagtgctt ccctttgcac aggccgcgtt tggttacgaa gttatagatg ccgccccggc
     1201 cctgcgcgtc acccggatac cagttctgca cggtcgagta tttgatctgc gcgttatcca
     1261 gcgcgattag ctccaccacc gccgcgtgca actggtgttc atcccgcatc ggtgccgtgc
     1321 agccttccag gtagctcacc gaagccccct cctcggccac gatcagcgtc cgctcaaact
     1381 ggccggtgtt ggccgcgtta atccggaaat acgtggacaa ctccatcggg caccgcaccc
     1441 ctttggggac gaagcagaac gacccgtcac taaacaccgc tgagttcagc gccgcaaagt
     1501 agttatccgt gtaaggcacc accatcccga gatacttctt caccaggtcc ggatgctcct
     1561 ggaccgcttc gctgaacgag caaaagataa tccccttctc ggccaactgc ttcttgaacg
     1621 tggtccccac cgacacgcta tcaaacaccg cgtccacggc caccccagcc agccgcccgc
     1681 gttcgtgcaa cgggatcccg agcttctcgt aagtctccag cagcttggga tcaacgtcct
     1741 ccaggctccg cggcccgtcg cccttcttct tcggcgcgga gtaatacacc gaggcctggt
     1801 agtcaatcgg cggatagtgc acctgcggcc acgtcggctc tttgagcgtc agccaatgcc
     1861 ggtaggcctt cagccgccag tccaacatga actccggctc cttcttcttc gccgagatga
     1921 aacggacaat gtcctcattc aagcccggcg gcgccgaatc ggcctccaca tcggtgtaga
     1981 agccgtactt gtattccttc ccgactaagt tttcgattgt ctcggttgct gtgctcatct
     2041 cgattctaac caatgtaacc catccggctc attccactcg ccaaatcctt ttctcgcctt
     2101 ccgcctttgc acgccgggca ccggccgtga atagcgatat cataccattc cgcccggtag
     2161 cctttgggca gggcaatccc cgtctccgcc ggcagctcaa tatcaaacac tttctcgcac
     2221 tggtcgcaat aaaagtggca gtgctcccgc atattcgggc agaagcgggt tgcgccccgc
     2281 tccagcgtca cttgccgcgc cagcccgcat tcgaccagcg catccaggca gttatagacc
     2341 gtcgccatgg agatatccgg gatctcgcgc ttagcccgga taaacacttc ctccgccgtc
     2401 ggatgatcgc gtttccgcaa cagcacagca tagacatgct cgcgctgcgg cgtaaaacga
     2461 aaaccgcgcg tcgccagccg ttcgttcagc cggttatcgg tttcttcagg caccgtcttg
     2521 accatggtag cgacaaccta gcccgagcct ggatcttgtc aatatttgga atagttctaa
     2581 accttgggtt ggataccggc ccaggccgcc cactggccca tgatctcaag ggccggggaa
     2641 gtgcggcggg cggccttgca tccgcggatt gcgcatccgc gttccatgtg ttgcacggcg
     2701 acgggggcgc catacaggtt ttgcatatct acatacgcca cctggaatcc ccggccaagc
     2761 agcgcgatat ccgcctgtgg ttcgtggccg aaaaactccg tgcgccagat ccatgggctg
     2821 ccgggcgcgg gcgccttcgg tctcacgacc agcgcggcgc gttctcccag cacttcccgc
     2881 acgccttgac cttctcttcc acgcgttgcg tgagaaccag tttccagccg acacaactga
     2941 cgtggcggat cttcggagct acgtgacgca agaaaccctc ccctggcatg gcagaggagg
     3001 ggtaacggag atcatggcga gcaaccagct cgataagccg agccaggcaa cgccgttctg
     3061 agccagccca ctacggtgtg tagaagagct gataaaagcc cttctggccc gcaggaatgg
     3121 gccaggtttg gctggtggag gtggcctgga tcgtggtcgt ggtccactgt gagaaatccg
     3181 aggtctgccg gagtgtgtaa gtggttccgg gcaggaggct atccatctgc agcgtcaggg
     3241 cactcccgtt acgaacgaca gtcagcactg gcggaacggt aatctgaata tcatcaacgg
     3301 cgaaagaggc ggagagcgcc gtggaatcag ccgttcccat ccagccttcc accaggactc
     3361 gccattgtac gaggttgccg gtatccacga ctgcgccgct ccagaactgg ttgaagttgg
     3421 cctggagcct ggtccacccg gaggagatca ccggggcagc cggtgtagtg aaattgaggg
     3481 cattcccgcc ctggtcgtag aacgagaggt agaagaccgt cccgctgtcc gcgacggggg
     3541 ccagggtggg atcgcctttg agccagagcg agactacggc gttggtgggg aacgaaaacg
     3601 gagccacgac ggcagagtaa acagcgcccc acggccattg gccggcaccg aaatcaatgg
     3661 cgagcttcag ggcattggtg ccttgagggg ctggcgtttc aagcgaggcg gtagtgacgg
     3721 tcgtaggcgc gccattcacg taatggtaaa acccgaggag agccgttgca tccgcgtact
     3781 cctcgaagtt gtcaatcttt gcccgcatgg gggagggcgg cgggaattcg atcaacggcg
     3841 agttgcgcac ctggagttcg tcaagataga tggtggcggt aaacgcattg gccggatcgg
     3901 gagcgccatt ttggccgtag aggtagaatc tgaattgcgc gatgttggtc aagtcaggag
     3961 ccccaggaga gttccagggc acttcaacgg agctcgctgg gaagttgaaa acctgccaat
     4021 tggtggtggt cgtggggacg gctgagcccc agcggccaaa gttgccattc ccatcccagg
     4081 cgtaaacata gatcatgtta aaagtgctgt tcgtgtactg ggggtcgccg gcaattctga
     4141 gagtgatgta ttgagtaggc gagatggcga ttggagccgg gagtggctgt gtgttaagta
     4201 ctgtggtctg ccaggcattg gcgggcacgg atatatccac gcgcatggag ttcgtgcctg
     4261 tcgagcgata agcaacgtag gacgagagag tcaacgttgc gctcatgtca ggggaccaca
     4321 cagcctgcaa atcggcatca ctgggatatt cctcgaagtt ttcgagcatc atctgcgcgt
     4381 tggccgtcag gccgaacaag cagccacata gcatgctaac acatagggat tttgttgtag
     4441 tcttcataag gacataaggt gtactagatt ataccgacga catcgcgagt ctagcgggca
     4501 ggcgtatcct gtcaagcctt tcctcaacat tttgaaaagg ctgcctttct gattctaagg
     4561 ggctggccaa ggcgcctaat gggcttggac tattgagggt tcgattattg accttgcttt
     4621 tgtcacccgg taatccaacc cttgcccagc gcgcatatga aaccgtccag ttttcaaggc
     4681 tgttcgtggg cggccttcac aggctgtttg ccgcgcatgg gaagcatgga ccctggggga
     4741 acatccaaca cccaacatcc aacatcgaac atccaatggc ctcggtttgg gcgatctttg
     4801 gatgttgggt gtgtttcatg gaaagggctc gggttccttt ggccaagggg gtggttacgc
     4861 atggccacgg tgtggacggt ggcgctattc ctcttcggcc ggggccacag ccttggcgca
     4921 cctctcatta ttgacgattt tgcttacacc aactcggcgg cggcccgcca ggcctgggtg
     4981 ggggtcagcg cgcccgcggt gaacatggcc gcctccggag attggggcac cgaccaggtc
     5041 atgaccctca cctgcgattt tgccacccgc gacgtgcggt gttattggga ccgcacggtc
     5101 agcctcaacc tggcggcctt caccgacttc gtgctggagg tgtttgcgcc cgatccgggg
     5161 gcgatttcct cttttacgct ctacttccat tccggcgcgg gttggtatgg cggctccgcc
     5221 tcgctcagcc aaacgggttg gcagacgttg cgcttctcca aggctagctt cattcctgag
     5281 ggcacgccct cgggctggga ccagattgac ggcattcgcc tctcgccctg gaaaggcgcg
     5341 gcgcgaaaca cctacctggc ggcgcgccag ttgcgggcct tcacgccgcc ggtgctgttg
     5401 gtgcgcgatg acgacagcag caacccgacc gtcgtgcagc aaactatcgc cctgcacacg
     5461 caatggctcg gaggttacaa cattgactgt ggcgtgctgg cgcgcacgaa tgtcgaggcc
     5521 gggctgctcg cgcaaagccg gctcgtgatc ctgccctaca acgaaaacat ctccgctgcc
     5581 gagtggaccg ccctggaaag cttcgtcgcc gccggcggca agctcctggt ctattacctc
     5641 ctgcccgcgc gcatggaagc gttgctcggc gtgcgcgcga cgggctgggc cgcgggagat
     5701 ttcgccgcct gggtttttgc cgacccggcc atcccgggct tgccggcgcg ggtgcaacag
     5761 gcctcgtgga acatcaccta cgccgtgacc aacgggctca actcccgcgt cagcgccacc
     5821 tgggtggaca gccacggcgc ctcaaccggc cacgcagcgt ggctgaccag cgaccatggc
     5881 ttcttcatgt cccacatcct gcttgatgac gatcctgatc agaaagccta cgcgctactc
     5941 tgtttggtcg gctatttcct gcccgacgtc tggcctgccg ccgccgtcgg cgccattgac
     6001 gggattggcc gtgttggacc gtataacacc tatgccgaag ccgtggccgg catccgccac
     6061 gacgccgaaa gcaccctccg cgcgcccctg gcagaggctg aacttgccac cacggcggct
     6121 gaacgcgatc gcgcgctggc cggggtcgcg gcaacgaatt acccgcaagc catcctcgcg
     6181 gctcaatccg cgcaggacca tttgagacag gcttacttcc tgagcctgaa gccggtcacg
     6241 ccggagttgc gcgccttttg ggaacaccac gccaccggcc cgtaccccgg caattgggct
     6301 gcggccgccg acgccctcgt gaccaacggc ttcacggccg tctttcccaa catgctctgg
     6361 ggcggtctcg cccactacaa cagcgctttc ctgccgcact cgaccgactt caccaactat
     6421 ggcgaccaga tcaccgcctg cgtcagcgcc gcccacgcgc gcggcctcca ggtccacgtg
     6481 tggaaggtca actggaacct cagcggcgcg ccccagtcct tcattgacag cctgcgcgct
     6541 gccaaccgca cccaggtctc cagcagtgga gtggcaatag actggctctg cccctcccat
     6601 cccgacaact tcgccctcga aaccaattcc atgctcgaag tcgtccgcaa ctacgatgtg
     6661 gacggcatcc acttcgacta catccgctat cccgacagcg attactgcta ctgcaccggc
     6721 tgcgccgccc gtttccagag ccagaccgga caagccgtca ccaattggcc tgccggcgtg
     6781 ctcgcgaccg gcgcgctgcg caactccttc ctcgattggc gccgcgctca gatcacccgc
     6841 ttggtcaccg ccgtccacgc gggcgtcaag gccatcaagc ccaaagtaca ggtctccgcc
     6901 gccgtgttcc ccgacgccgc gagcgccttt gacggcgtgg gccaggattg gcgtgcctgg
     6961 attaccaacg gcatcgtgga tttcctctgc ccgatggatt acaccacgag cctcagccaa
     7021 ttcaccaacc ttgtcgccgg gcaactcacc tacgccgcgg ggcgcgtccc aatttacccc
     7081 ggcatcggcg cttatgtgct ccagccagat gccgctctcg cgcagctcca gcagacgcgc
     7141 gccgccaaca cacacggatt catcatcttc gaactcagcc ccgaagcggt cacgaatctg
     7201 ctccccgcga tccgcgccgg tgccaccgcg cccgatgaac cggacaccga taacgacctc
     7261 ctccccgaca gttgggagct gcgctggttc accaaccttt ccacggcggg cctgaacacc
     7321 gataccgacg gcgaccggct gaccgaccgc gatgaatacg tgctcggcac caatcccacc
     7381 caacctaatc ccggccttac cctgcaagcg cgcctggtcg gcggcaccgt cgaagtttcg
     7441 tttctggccc aggcggtcgc tggcgccggc taccagaacg ccgagcgcca ttactgcctc
     7501 gaaagcagga cgaccctcgc cggcgggagc gtttgggaac ccgtcgccgg cttcgctgat
     7561 cgcaccattg cttccggttc cgagactttg gtcttctccg tttcccccgc cccgacccag
     7621 tccgccctat tccgcgcccg cgtctggctc cagcagaaac aacgctagtg cttggttgca
     7681 caagcaaccg atataagagg cgccgcgtga gatggataca gaagcgcgtt ccggcaccaa
     7741 atcgggcgct ggaaagagca cttcaaccgc tggactttga gcctttacgc gcttctcgcg
     7801 cccgcgtagg ctgcgagcca cgaatgaaag ttgatcgagc ttcctggctg agccggcgtt
     7861 caagaaaagt ggcccttgcc gcctgcctat tgcagctcac tggtttcggc tcattcgcgg
     7921 ctgcaggggc tgagcccacg aacctggatt cagatcccat ttcgttgctg ctggccgcca
     7981 ccttcaagat caccaacaaa caatcttccg ggacctgctt ccttattgaa ccgccgccgc
     8041 cctccgggtg gtcaaatgga gtcgtcatct tggtcactgc cgctcacgcg ctggaagagg
     8101 cccctggcac ggagtgccgc atcgtgatgc gcgaaaaaga cgccgcaagt agttaccttc
     8161 gcaaggaagt gcctttgacc attcgctccg gggagaaagc gctctgggtc aagcacccgc
     8221 atgaagatgt cgcagccatc aagtttacat tgcccttggg agtttcgtgc cagcctcttc
     8281 gcctcgacca attggcccgg gcggacgatt tctccaacgg caagatccgg ctggcgagtg
     8341 acacctggat cttttgcttt ccagcgcact tggaagccaa cgacgccggg ttgcctgttc
     8401 ttcgccatgg ctcgatcgcc agtttgcctt tgatcccgct ctcatccaac cggaccttcc
     8461 tcgtggactt caacacgttt ggtggcgata gcggggcgcc cgtgatgatg ggggcccgcg
     8521 gcgcgatcca gcctcaagcc ctgattgttg gtttggtcct gggcatgcaa cgccagacgg
     8581 ataaggcctc gatgcccttt gaggagcgca cagttcacca tcccttggca ttggccatcg
     8641 tggcccattc ggaaatcatt cgccagacag tggaactcgt cctcagatga tttcgatgat
     8701 ttgcacgaga ctggcgcacc aactcgatta cgccttaccc gcgtagcgct gagtacatgc
     8761 gggcgcgcca aaaaccaaaa aacactgaac cacgaacctc gaacctcgaa cagccgggca
     8821 aagccacccc aaagccaccc caaactctgg gcgcggaaac cacctgtctt ccctgagctt
     8881 tggccat
//
LOCUS       DATCGS010000420         4199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_185178, whole genome shotgun sequence.
ACCESSION   DATCGS010000420 DATCGS010000000
VERSION     DATCGS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185178"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..647)
                     /locus_tag="VIL39_08640"
     CDS             complement(84..647)
                     /locus_tag="VIL39_08640"
                     /inference="COORDINATES: protein motif:HMM:NF020845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5481530.1"
                     /translation="MQTVLPAEFKRGMLLLLDNIPHLIEDSHTSGTAQTRHKLHVRVR
                     NLKTGNVIERTFAENERLPVGELEHRTVQFSYKQGDTYAFVDTKTFEEMHLSAELAGE
                     RRWFIKENEEYKALFLEGNLIDIVLPPQVALQVVDTAPPSRGGLESSWKTAKLETGLE
                     LMVPLFITKGEMIRVDTQERKYAGKSN"
     gene            1002..2267
                     /locus_tag="VIL39_08645"
     CDS             1002..2267
                     /locus_tag="VIL39_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481531.1"
                     /translation="MPVLCARRFCFGWVSLVLFTVLHDSQAALPLKAAITTNAAPAGF
                     VEVSGVEAGEFNPLIWQAGTLNFVPDLRAPLLAPRSGKFRNIYAPSAVETPGGYRLFY
                     GAWDGVPTGNDRIYSARTDTRFQDFSDRHIVIAPGSYQHVCNVNALRAEDGSFTLFAT
                     LYPIRNLNKPAFFQSDTSGTNWNGLPGEPYTVQARDVVDIGGYAYTNADINGMNVMLR
                     EGGVYRLYFGDFRNPGGTFRATSTDGKHYTLDAKVLAGSGFVNDVKKFRVGGTSYYLM
                     GLHENGTRLWQTVSTNGTAFPPARMLATNLGADDAYIVALGWVVRGPQESPGRKLLGV
                     LYGAGPVSALNQNSIYARWLQKRVVFVANDGTRYPGTKALGPDRQLLAVPDAGPVTGR
                     FEVYGEDGVTLIGVSATQAARRGQTFRLW"
     gene            2460..2813
                     /locus_tag="VIL39_08650"
     CDS             2460..2813
                     /locus_tag="VIL39_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481532.1"
                     /translation="MKAGLQVVVAVILTAGCAQPSRFQNDPRAAVVGPGYLDCIEKYP
                     RDRHTQYYYPAFMVHGLTNWVYPFVGLQSAVSPDIQKLETSIPPMPPSVQAIEGKGWP
                     ELERKGLPLFLPKQQ"
     gene            complement(2854..4041)
                     /locus_tag="VIL39_08655"
     CDS             complement(2854..4041)
                     /locus_tag="VIL39_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEY5481533.1"
                     /translation="MKTKSFSRRAFIQQGFALAAAPLVFTYTRTGLLAAETAGSPAQS
                     LAGAHRATAKVAIAPCRSYGPEVRPALEKCFDQLGGIGSLVKNKTVTVKLNLTGNSAK
                     PLLGRPIGETYTTHYATALALSSLLFAAGARRVRLVESTQSRNGLESTLTEAGWDVPA
                     LTALGKLEYENTRNLGQGKKYSTLRVPGGGYMFSAFELNHAYEDTDVLVSLAKLKNHL
                     TAGVTLSMKNLFGLPPNSLYGTEAGKETATGPRFPLHDPKGYESMKLSGLKEGITSTD
                     PSWRVPRITVDVCGARPIHLAIIDGIMAMSGGEGPWSGEERLKITTPGVLIAGLNPVS
                     TDAVATAVMGYANPRAARGVKPFQDCDNHLLLAEQAGLGTADLAQIDLRGMPIEKARY
                     PYG"
BASE COUNT          749 a         1293 c         1321 g          836 t
ORIGIN      
        1 gttgggaaat ccattactcc atcactccaa tactccacca ccccacccac cccaccactc
       61 caccactccg ttcttgagtt ctctcaattg ctcttgccgg cgtatttccg ctcctgggtg
      121 tccacgcgga tcatttcgcc cttggtgatg aacagcggca ccatgagctc gaggcccgtc
      181 tctagtttgg ccgtcttcca ggaggattcg aggccgccgc gactcggggg cgcagtatcc
      241 accacctgca gggccacctg cggcggcagg acgatgtcaa tgaggtttcc ttcgaggaac
      301 agggccttat actcctcgtt ctccttgata aaccagcggc gttcgcccgc gagctcggct
      361 gaaaggtgca tctcctcgaa ggtcttcgtg tccacgaacg cataggtgtc gccctgtttg
      421 tagctgaact gcaccgtgcg gtgctcaagt tcgccgacgg gcagccgctc gttctcggca
      481 aaggtgcgtt caataacgtt gccggtcttg aggttgcgca cgcggacgtg cagcttatgc
      541 cgggtttgag cggtgccgga ggtgtgcgag tcctcgatca aatgcgggat gttatccagc
      601 aagagcagca tcccacgttt gaattcagca ggcagaacag tttgcatagg gctttcctcc
      661 ggcctggggc cggcgttgtt acggcacgac cagcgccgca ggttaatggg atggttgtac
      721 ctccaatcat cggcgccgtc aactgcgcag aaggagcggc gaatggggcg ggggcatctg
      781 ccgcggactt acaccaccac agacgcctcg ggccggagtg cgcccgtcct cgggcgcagc
      841 aagctccgac ccgcccgcga cgcttggaag ttcctagacc gcactgcgca cggacgtcgc
      901 tgcgcccgag gacgggcgca ctccggcggc acggcgcaga tactcccgaa tggggcttgg
      961 ccgcgcgggg cggtttgtgc cagcctgtgg agatgcgatt tatgcctgtc ctgtgtgccc
     1021 gacggttctg tttcggctgg gtgagtctgg ttctattcac ggtcctgcac gattcccagg
     1081 ccgcgctccc cctgaaggcg gccattacga cgaatgctgc gccagcgggt tttgtggagg
     1141 taagtggcgt ggaagccggc gagttcaacc cgctgatttg gcaggctggc acgctcaact
     1201 tcgtgccgga cctgcgggcc ccgttgttgg cgcctcggag cgggaagttc cgcaatatct
     1261 acgcgccgtc ggctgtggag acgcccggcg gctatcggct cttctacggt gcctgggatg
     1321 gcgtgccgac tggcaacgac cgcatttaca gcgccaggac ggatacccgc ttccaggact
     1381 tctccgatcg gcatatcgtc attgctccgg ggagctacca acacgtgtgc aatgtcaatg
     1441 cgctccgggc tgaggacggg tcgttcacgc tgttcgcgac gctttacccg atccggaatc
     1501 tgaacaagcc tgcattcttc cagagtgaca cctcgggcac gaattggaac gggctgccgg
     1561 gcgaaccgta caccgtgcag gcgcgagacg tggtggacat tgggggctac gcctatacca
     1621 acgcggacat caacgggatg aacgtgatgc tccgcgaggg cggcgtgtat cggctttact
     1681 ttggtgactt caggaacccg ggcggcacgt tccgcgccac gagcacggat ggcaaacact
     1741 acacccttga tgcgaaggtg ctggcgggtt ccggcttcgt gaatgacgtg aagaagttca
     1801 gggtcggagg gaccagttat tacctgatgg gtttgcacga gaacggcacg cgtttgtggc
     1861 agacggtttc caccaacggg actgcatttc cacctgcgag gatgctggcg acgaacctgg
     1921 gagcagacga tgcctacatc gtcgcgctgg gctgggtggt ccgcggccct caggaatcac
     1981 ctgggcggaa gttgttgggg gtcctgtatg gcgccgggcc ggtgtcagcg ctcaaccaga
     2041 atagcatcta cgcgcgctgg ctgcagaagc gggtggtctt cgtagccaat gacgggacgc
     2101 gctacccagg cacaaaggcg ctcggcccgg accgccagct cctggctgtt cccgacgccg
     2161 gcccagtcac cggcaggttc gaggtctatg gcgaggatgg cgtgacactc attggcgtgt
     2221 cagcgacaca agccgcccgc cgtgggcaga cctttcgcct ctggtgaccg acggagtctc
     2281 gcacagagca agcgatcagt tagtctcctt ccccgcctca ctgcgttaca tcttcgacgc
     2341 tcccgtcagt tcaatcccag gggcagcgct ttcgttctgc ttcctttagc ctggcaggga
     2401 tcacaacgct ggcagaagac ccctttacaa cccgggagac ttgagccagc atgatactca
     2461 tgaaagcagg attgcaggtg gttgtggccg tcattctgac cgcgggctgt gctcagccga
     2521 gccgattcca gaacgatcct cgggcggctg tggtgggacc gggttatctg gattgcattg
     2581 agaaataccc gagagaccgg cacacccaat actactatcc cgccttcatg gttcacggat
     2641 tgaccaattg ggtatatccc tttgtgggct tgcaaagcgc tgtttccccg gacatccaaa
     2701 agcttgagac ctctatccca cccatgccgc cttccgttca agcaatcgaa ggcaagggct
     2761 ggccagaatt ggagcgcaag ggattaccgc tgttcctgcc caagcagcaa tgacctggta
     2821 accactctcc tgccaccacg aacttcggtg ccctcaaccg taggggtaac gcgccttctc
     2881 gatcggcatg ccgcgcaggt cgatttgcgc caggtcggcg gtgcccaagc cggcctgctc
     2941 ggccagcagc aggtggttgt cacaatcctg gaacggcttc acgccccggg cggcgcgggg
     3001 gttggcataa cccatgacgg cggtggcgac ggcatcggtc gaaaccgggt tgagcccggc
     3061 aatcagcacg ccgggcgtgg ttatctttag tctctcttct ccggaccagg ggccttcgcc
     3121 gccgctcatg gccataatgc catcaatgat ggccaggtga atgggccggg ccccgcacac
     3181 gtccactgtg atgcgtggca cgcgccacga cgggtcggtg gaggttattc cctccttcaa
     3241 acctgacagt ttcatactct cgtaaccctt gggatcgtgg agcgggaagc gggggccggt
     3301 cgcggtttcc ttgccggcct cggtgccata gagcgagttc ggcgggaggc cgaagaggtt
     3361 cttcatcgag agcgttacgc cggcggtgag atggttcttg agcttggcca aggagaccag
     3421 cacatcggtg tcctcgtaag cgtggttgag ctcgaaggcg gaaaacatat agccgcctcc
     3481 cggcacgcgc agggtcgagt attttttgcc ctggcccagg ttgcgcgtgt tctcatactc
     3541 gagcttgccc agggcggtca gcgccggcac atcccagccc gcttccgtca aggtggactc
     3601 caggccattg cggctctgcg tggattcgac caggcgaacc cgccgcgcgc cggccgcgaa
     3661 cagcagcgaa ctcagcgcca gcgcggtggc gtagtgagtg gtgtacgtct cgccgatcgg
     3721 ccggccgagc agcggcttcg cgctgttgcc ggtcaggttc agcttcacgg tcacggtctt
     3781 gttcttgacc agcgacccga tgccgccaag ctgatcgaaa catttctcca gcgccgggcg
     3841 gacttccggc ccatacgacc ggcaaggcgc aatcgccact ttggcggtag cacggtgtgc
     3901 cccggctaag ctttgggcag ggctgcccgc ggtttccgcg gcgagcagcc cggtgcgggt
     3961 gtaagtgaat accagcgggg cggcggccaa cgcaaaccct tgctggatga aagcccggcg
     4021 gctgaacgat ttcgtcttca taagacaggt cgaataccat ttaggatgct cgctctactc
     4081 tgcctcgaac ttgtcgccag tcaagtgtgc ccgcctgcgc ggcgtgagga cgggcgcttc
     4141 ctgacaccaa caccaaggcg ggcggccatg ccaatcggaa cgccggattc atccggcag
//
LOCUS       DATCGS010000421         9137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_531866, whole genome shotgun sequence.
ACCESSION   DATCGS010000421 DATCGS010000000
VERSION     DATCGS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531866"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1470..2360
                     /locus_tag="VIL39_08660"
     CDS             1470..2360
                     /locus_tag="VIL39_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5481534.1"
                     /translation="MKRILSACLLLLALSCAWAQTNVDFTRTEDVIYGRKFGTALTLD
                     VFQPRTANGVGIIYMVSGGWFSAHEAINEGYFRPLLNRGYTVFAVVHGSQPKFVITEI
                     VPDINRAVRFIRHNAARYGVDPNRFGISGSSAGGHLSLTMGTQGGPGDANAKDPVDRE
                     SSAVQCVACFFPPTDFLNYSQPGEDAVGVGVLKSFKAAFGPRSDTAEERQELGREISP
                     IYFVHSNMPPILIVHGDADKLVPICQSETFVKRCEAVGSTAKLVVREGKVHGWPDMGK
                     DMELFADWFDEHLRGIKPKL"
     gene            complement(2370..4532)
                     /locus_tag="VIL39_08665"
     CDS             complement(2370..4532)
                     /locus_tag="VIL39_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5481535.1"
                     /translation="MKTEDYLALDFQDSMGLLSQSRREFLKRLGGGIFIFVSLGELVT
                     AQEAGRQRGGGQGLPSDFNAFLRIGEDGRVTCFTGKIEMGQGPITSLPQMLAEELEAP
                     LDTVDIVMGDTERCPWDMGTFGSMTTRSFGPALQAAAAEARAVLLELAAEALKVPQAQ
                     LVAKDGVIFDGQNKEQRRTYGQLAKGQTIERHLQSKPALKDVAKHTVVGKPLVRRDGR
                     DKVTGKAQYAGDLREPGMLYAKILRPPAHGAKLKSVDTAPAKAIAGAQVIQDGDLVAV
                     LHELPDVAEAALDKIKAEFDVPEPTVDDKTIFDHLLKTNPQGRAVAEGGDLAEGRKLA
                     SKTFEQTYLNSYVAHTAMETHTALAKVEGDKATVWASTQNPFGAQREIAEAIGLPAQK
                     VRVITPFVGGGFGGKTRNTQAVEAARLSKAVGKPVQVAWSRAEEFFYDTFRPAAIVKI
                     SSGIDGSGKMAFWDYDVLYAGERGAKQFYSVANHRTTARPSGWQGPPGSHPFATGAWR
                     APSNNTNTFARESQIDIMAAAAGMDPVDFRLKNLIDPRMIRVLKAAAERFGWTPAKAP
                     SKRGYGVACGMDAGTWVATMIEAEVDAKKGTVQVKRVVCAQDMGLVVNPEGAKIQAEG
                     CTTMGLGYALTEEVHFKGGKVLDTNFDTYELPRFSWVPKIETVLIDAKDSPPQGGGEP
                     AIIVMGAVIANAIYDRTGARLFQLPMTPERVLAAIQKA"
     gene            complement(4535..5035)
                     /locus_tag="VIL39_08670"
     CDS             complement(4535..5035)
                     /locus_tag="VIL39_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5481536.1"
                     /translation="MAVRLEKSMSDAITFKLNDKPVRLNVDGDRALLWVLRTDLGLTG
                     TKFGCGEGHCGSCTVLVNGVAVRSCLRKVKEVKDKEVVTIEGLAKGGVLHPVQKAFLE
                     HDALQCGFCTPGMVLTAVGLLTSNPKPTREQILRGMEQNLCRCGAHTRIVDAIQSAAG
                     EMKGGQ"
     gene            5214..5936
                     /locus_tag="VIL39_08675"
     CDS             5214..5936
                     /locus_tag="VIL39_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5481537.1"
                     /translation="MGAQWKQAGREEHAQKKGQMTVKLVREIMVAAKLGGPDPDLNAR
                     LAAAVEKAKKASCYRDTIERAIKKGSGQTDEKVNFELVTYEGFAPHKVPVVVECLTDS
                     RNRTAPEIRNIFKAGTLGQPGSVAFFFNHLGVVEATHTDVNRDAEGDAIEAGAQEIEA
                     LEAEEIPAGQKGARFLTEPKELDHVSKALKSAGWNIISSEMRYLAKTFPDLNEAARKE
                     VADFLNALDDHDDVHRVYAAMK"
     gene            5991..7571
                     /gene="amrB"
                     /locus_tag="VIL39_08680"
     CDS             5991..7571
                     /gene="amrB"
                     /locus_tag="VIL39_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5481538.1"
                     /translation="MALAKQPMRHQGLIALALLLAGFGCGARPEAARDAQSTNSAGPA
                     SSAERSQRVRAPAVAGLFYPKDAAALSKTVDGLLVGAPEHHIARLKGLVCPHAGYEYS
                     GQTAAIAYKLLAGRDVQTVVVMGPSHYAGFQGACIPNADAYQTPLGLVPISEKAKALA
                     LVPPFVLEPQCLVQRPGWWRQASKPAPEAGQDTPETWEHSVEVQVPFLQKVLKDFKLL
                     PVVIGEADPAQVAKALAGNIDDKTIVVASSDLSHFHTSQAAKGLDDRCVKAICDLDVD
                     AMAAQEACGKLPILTLLHLARQKGWKAQLLDYRNSGDVTGEKDSVVGYTAVAFYAPVP
                     ENLSASERKFLLELARKTLVTVTAGGSVPAVAEKDVPPKLAGKQACFVTLTKDGALRG
                     CIGHLTAMEPLHQAVAENARNAALRDPRFSPVQSEELDKIKIEISVLTEPQPLAFSSP
                     DDLLSKLHPNEDGVLLHIGPRTATFLPQVWAQIPDKVKFLEYLSQKAGGEASAWRGKD
                     VSVAIYHVECFEEEHSRP"
     gene            7534..8430
                     /locus_tag="VIL39_08685"
     CDS             7534..8430
                     /locus_tag="VIL39_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481539.1"
                     /translation="MLSASKKSTPVPDRQGLVLTAVGALGVSCVMTQLALMRELLGAF
                     AGNEMVLGVVLGLWLLLMGIGAWLGHNSDKLRHQLTALALIQMLVALLPLAQVFLLRA
                     LRNVVFIRGAAVGVAETVGASFVLLLPYCLVAGYALTLACGILARQQGPAGIGRAYVA
                     DSIGSIIGGALFSFVLVRSFDHIAILAYPALLNLLLAGLLGSGVQGLKSKVQSSKFAS
                     LLLPASALILGLGLIATILLFDLDDLSTRLQYPRQHIVARANSPYGKLIVTESYGQFD
                     FLENGIPITSTRDDEHVEETVH"
     gene            8434..9033
                     /locus_tag="VIL39_08690"
     CDS             8434..9033
                     /locus_tag="VIL39_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481540.1"
                     /translation="MAQRPEARRVLLVSGGVSGTAREILKYGVPAVDYVELDPLILVL
                     GRRYVPANLADGRIRVINTDGRLFVKQTPEKYDVVIIDVPAPATAQLNRFYTVEFLAE
                     VKRVLARDGVVGFALGQYENYVSPELARMLSSACLSAKQSFRNVLAIPGGRVFFLASD
                     GPLYANIAARIEQRHVKTKLVNRHYLDGTASAFRKPFVW"
BASE COUNT         1677 a         2901 c         2817 g         1742 t
ORIGIN      
        1 agccaggcga ggccgaactc gaacgccagc tcgcttcaaa aggtgggcac acgcgcggct
       61 atttgccgca ttacaataag cccggcactt tgcagatgct ttgaggaaac tgcgtgcttg
      121 ggtggagggt aaattggcct cggcagaaaa accggggcat ggctaacccg catattgcac
      181 aggcgaacaa agggcacagt gtatggagtc ccggctttag ccggccagat gcggtttcgc
      241 catggatctc caaatgcttc gagccgtctt caagggacaa caccctgccg gctaaagccg
      301 ggactcgtgc gcctgagagc gcactggaac agcagggtgg aagtccctga cctggccgac
      361 actgaagggc cagtaactga aagtaactgc atcggggcga tacccccggg tggagagcaa
      421 ctggaagtga accaccagtc cgtaacggtg accgcccgta tgggcggaca gagtgaactc
      481 gattcggtcg gcggttctgg cgagcctgcg agtccagggc gaagcccgaa caaaatagtg
      541 cggccagcgt tgagggagtg gaacgcgtct ggtcgagaac cgcagatggt tggagacaga
      601 atggtgggaa gttctggtga cgtgaatcag ggaaccacgg tgggtgatga cgaaagggcc
      661 tgccggggtc agagcgtccg tagtagcgaa gaagcgagta atgatcgtgg agccaaggga
      721 cgtaggaagg cagtgctggg agccgaactg aggccatctc gcgagggccg acgtagtgcc
      781 gtaaggctgt gcgcgtcggt gcggcggaaa accgcttggc tgaggaagga ctgctggcag
      841 tgggccgccc ataacagggt gagcgggtcc gcaaggacag cgcggctggt gtgttccctc
      901 cgagcatctc ggaacactta cctaagccag cccatcggtg gctgccgact ggaaagccgt
      961 atgcaggaga tctgcctgtc cggtttggga gggaggggga gcgcactgcg ctcctcctat
     1021 cctcatcata ccccgcgcaa agcccacatt cgttatcgaa ccgtgcgctc actccttccc
     1081 ttatatctgg gtcagtcggg cggtgcgatg cactgtcccc gtcaactgcg gggccaaagt
     1141 tcgtgccagg gtcccttccg atcgcctttg cccagcgcgc acactgccca gacccgccca
     1201 accccgccag cgccccttgc gcatcccttg ttccgccggc actgcaagcc aggtgcaagc
     1261 ggaatgcaag ggcgacgctg gcgcgttgct aaggtgttgc tctggtgttc ccttcgtatt
     1321 ctcttcgtat tctcttcgta ttctcttggt gttttccgcg tattcctatc ccgtctgtgc
     1381 cccaactcgg cggcggcgct agcacaaccc tgcgacgagc cggcttgtcg ccggcaaggc
     1441 agctcgatag actggcggga atgcatgcca tgaaacgaat actctccgcc tgcttgttgc
     1501 tgctggccct gtcctgcgcc tgggcccaaa ccaatgttga tttcacccgc accgaggacg
     1561 tgatctacgg gcggaagttc ggcactgccc tgacgctcga cgtcttccag ccgcgcacgg
     1621 ccaatggcgt gggcatcatc tacatggtca gcggcggctg gttttccgcg cacgaagcca
     1681 tcaatgaggg ttactttcgg cccctgctga atcgcggcta cacggtgttc gccgtcgtgc
     1741 atggctcgca gcccaagttt gtgatcacgg aaatcgtgcc cgacattaac cgggccgtgc
     1801 ggttcatccg ccacaacgcc gcccggtatg gcgtggatcc caaccgcttc ggcatcagcg
     1861 gcagcagcgc cggcgggcat ctttcgctga ctatgggcac ccaggggggg ccgggagacg
     1921 ccaacgccaa agaccccgtg gaccgcgaga gcagcgcggt gcaatgtgtg gcgtgcttct
     1981 tcccgcccac ggatttcctg aactacagcc agcccggcga agacgcggtc ggcgtgggtg
     2041 ttctcaagtc gttcaaggcc gcgttcggtc cgcgctcgga cacagcggaa gagcggcaag
     2101 agctgggccg ggagatttca ccaatctatt tcgtgcactc gaacatgccg cctatcctga
     2161 tcgttcatgg tgacgccgat aagctggtcc cgatttgcca gtccgagacc ttcgtcaaac
     2221 gctgcgaggc ggtgggctcg accgccaaac tggtggtgcg cgaaggcaag gttcacggct
     2281 ggccggacat gggcaaggac atggaactct tcgccgattg gttcgacgag catctgcgcg
     2341 gcatcaaacc gaagctgtga tggagtcact caggccttct gaatcgcggc cagcacacgc
     2401 tcgggcgtca tgggcaactg gaacagccgc gcgcccgtcc ggtcgtagat cgcgttggca
     2461 atcaccgccc ccatgacgat gatcgccggc tcaccaccgc cttggggcgg cgagtccttg
     2521 gcgtcaatga gcacggtctc aatcttcggc acccacgaga agcgcggcag ttcatacgtg
     2581 tcgaagttcg tatccagcac cttgccgccc ttgaaatgga cttcctcggt cagtgcatag
     2641 cccaatccca tagtggtaca cccttccgcc tgtatcttgg ccccttccgg gttcaccacc
     2701 agccccatgt cctgagcgca gacgacgcgc ttgacctgaa cggttccctt cttcgcatca
     2761 acttcggcct caatcatggt ggccacccaa gtccctgcat ccatgccaca ggctacacca
     2821 taaccgcgtt tgctgggcgc tttggccgga gtccagccga agcgttcggc ggcggccttg
     2881 aggacgcgga tcatccgggg gtcgatcaga ttcttgaggc ggaagtccac cgggtccatt
     2941 cctgccgccg ccgccatgat gtcaatttgt gattcccggg caaaggtgtt ggtgttgttg
     3001 ctgggcgcgc gccaggcgcc ggtggcaaaa ggatgggacc ccggcggccc ctgccacccg
     3061 gaaggccggg cggtagtgcg gtggttagcg accgaataga actgcttcgc gcccctctcc
     3121 cccgcataaa gaacatcgta gtcccagaaa gccatctttc cggagccgtc aatgccggaa
     3181 ctgatcttaa caatggctgc tggccggaac gtgtcataga agaattcctc agcccggctc
     3241 caagccacct gcaccggttt gccaaccgct ttggacaacc gggcggcttc gaccgcttgc
     3301 gtattcctcg tcttgccgcc gaaacctcca ccgacgaacg gagtgataac ccggaccttc
     3361 tgtgccggca gtccaatggc ctcggcaatc tccctctgcg ctccaaacgg gttctgggtc
     3421 gaagcccaga ccgtcgcctt gtccccttcg actttcgcca gggcggtgtg agtctccatc
     3481 gcggtatggg cgacgtaact gttcaggtag gtctgctcga aggttttgct ggcgagcttt
     3541 ctgccttccg caaggtcgcc tccctcagcc accgctctcc cctgggggtt cgttttgagc
     3601 aggtggtcga agatggtctt atcgtccacg gtcggctcgg gcacgtcgaa ctcagccttg
     3661 atcttgtcca gcgctgcttc ggccacgtcg ggcaactcgt gcaggacggc caccaggtcc
     3721 ccgtcctgaa tgacctgagc gcccgcgatg gctttggcgg gcgcggtgtc cacgctcttc
     3781 aactttgcgc catgagccgg cggccttaga atcttcgcat agagcatgcc gggctcgcgc
     3841 aggtcgcccg cgtattgggc cttgccggtg actttatccc ggccatcccg ccgcacgagc
     3901 ggtttgccca ccacggtgtg cttggccacg tccttcaggg ccggcttgct ctgcaggtgc
     3961 ctctcgattg tctgcccctt ggctaactgg ccataagtgc ggcgttgctc cttattctgc
     4021 ccgtcaaata tcacgccgtc cttggcgacc aactgcgcct ggggcacttt gagagcctct
     4081 gccgccagct ccaacaacac cgccctggcc tcggcggcag ccgcttgcag ggcgggacca
     4141 aacgagcgcg ttgtcatcga gccaaacgtc cccatatccc acggacagcg ctcggtgtcg
     4201 cccatgacga tgtccacggt atccagcggc gcctccagtt cttccgccag catttgcggc
     4261 aacgaggtga tcggcccttg gcccatctcg atcttgccgg tgaagcaggt cacccggcca
     4321 tcctcgccaa ttcgcaggaa ggcattgaag tccgaaggca aaccctggcc cccgccgcgc
     4381 tggcgaccgg cttcctgggc cgtcaccaac tctcccagcg agacgaagat gaagatgccg
     4441 ccacccaggc gtttgagaaa ctcccgccgg ctctgcgaga ggaggcccat gctatcctgg
     4501 aaatccagcg ccagataatc ctctgtcttc atatctactg gcctcctttc atctcaccgg
     4561 ctgcgctttg aatggcgtca acgatccgtg tgtgggcgcc gcagcggcat aggttctgtt
     4621 ccatgccgcg aaggatctgc tcccttgtgg gcttcgggtt actcgtcagc aggccaaccg
     4681 cggtaagcac catgcccggc gtgcaaaacc cgcactgcaa agcgtcgtgc tccaggaaag
     4741 ccttttgcac cgggtgcaac accccgcctt tggccaggcc ctcgatggtc accacctcct
     4801 tgtctttgac ctccttcacc ttccgcagac acgagcggac tgccaccccg ttcaccagca
     4861 ccgtgcagga cccgcagtgc ccttccccgc aaccgaactt ggtcccagtg aggcccaaat
     4921 cggtccgcag cacccacagc agcgcgcggt cgccgtccac gttcagccgc accggcttgt
     4981 cattgagttt aaaagtaata gcgtcactca tgctcttctc cagtctaacg gccatgtccc
     5041 cccgtcatcg gttccaggcc ggtttcaatt tcatgccacg gttagtctca acccgtttgg
     5101 ccgaattgta aaagggcaaa tcatccgggg caaatcatcc tgggggttga cggtcggccc
     5161 cgcgcactcc acactgtcac taacgaaacc gcaacaacag gagcttacgt aacgtgggcg
     5221 cacaatggaa acaagccggg cgcgaagaac acgcgcagaa gaaaggccag atgaccgtca
     5281 aattggtccg cgagatcatg gtcgcggcca aactcggggg tcctgaccct gacctgaacg
     5341 cgcgcctcgc cgccgccgtc gagaaggcga agaaggcctc gtgctatcgg gataccatcg
     5401 agcgggccat caagaagggc tccgggcaaa ccgatgagaa ggtcaacttc gagttggtca
     5461 cctacgaggg gttcgcgcct cacaaagttc cggtggtcgt cgagtgcctc accgatagcc
     5521 gcaaccgcac cgcgccggag atccgcaata tctttaaggc gggcaccctc ggccagcccg
     5581 gcagcgtcgc ctttttcttc aaccacctcg gcgtcgttga agccacccac accgacgtca
     5641 accgcgacgc cgaaggcgat gccattgaag ccggcgccca ggaaatcgag gcgctcgaag
     5701 ccgaggaaat ccccgcgggc cagaagggcg cgcgcttcct caccgagccc aaggaactcg
     5761 accacgtctc caaagccctg aagagcgcgg gctggaacat catctcctcc gagatgcgct
     5821 acctggccaa gacctttccc gacttgaacg aggctgcgcg caaggaggtt gccgacttcc
     5881 tcaacgcgct ggacgaccat gacgacgtcc accgggtcta tgcggcgatg aaataggccg
     5941 cccggctggc gcgttccgtc cgcccagccg ccgaatgaaa ggatgagact atggcactcg
     6001 ctaagcaacc gatgcgacac cagggcctga tcgcgctggc gctgctgctg gctggctttg
     6061 gatgcggggc gcggccggag gcggcgcgcg acgcgcaatc cacaaactca gccgggccag
     6121 catcgtccgc cgaacgcagc cagcgcgtcc gggcgccggc ggtggctggg ctcttctatc
     6181 cgaaggacgc ggcggcgctc tccaagaccg ttgatggctt gctggttggc gcgcccgaac
     6241 accacatcgc ccggttgaag ggtctggttt gtccacacgc gggttacgag tattccgggc
     6301 agacggccgc tattgcctac aagttgctgg ccgggcgcga cgtgcaaacc gtcgtcgtca
     6361 tgggcccgag ccactacgcg ggtttccagg gagcatgcat tcccaatgcg gacgcttacc
     6421 agacaccgct ggggctggtg cccatctcgg agaaggcaaa ggcgctggcg ttggtcccgc
     6481 cgtttgtgct ggagccgcag tgtttggtcc agcgccccgg gtggtggcgg caggcatcca
     6541 agcctgcgcc ggaggcgggg caggacacgc ccgagacgtg ggaacactcg gtcgaggtgc
     6601 aggtgccctt cctgcagaag gtgctgaagg atttcaaact cctgccggtg gtgatcggcg
     6661 aggccgaccc cgcacaggtg gccaaggcgc tggccgggaa cattgacgac aaaacgattg
     6721 ttgtcgccag cagcgacctt agtcatttcc atacctccca ggccgcgaag gggctggacg
     6781 accgttgcgt caaggccatc tgcgacctgg acgttgacgc gatggcggcg caggaagcgt
     6841 gcggcaagtt gccgatcctg acgctgctcc acctggcccg gcagaagggt tggaaggcgc
     6901 aattgctcga ttatcgcaac agcggtgatg tgaccggcga aaaggacagt gtggtgggct
     6961 acacggccgt ggcgttctac gcgcccgtgc cagagaactt gtctgcctca gagcggaagt
     7021 tcctgctcga actcgcccgc aagacgctgg tgaccgttac cgccggtggc agtgtgcccg
     7081 cggtcgccga gaaagatgtg ccgccgaagc tggcagggaa gcaggcctgt ttcgtcacgc
     7141 tgaccaagga cggagcgctg cgcggctgca ttggccacct gacggccatg gaaccgttgc
     7201 accaggccgt ggctgagaac gcccgcaacg ccgccttgcg cgacccgcgc ttctcgccgg
     7261 tgcaatctga ggagctggac aagatcaaga tcgagatcag tgtgctgacg gaaccgcagc
     7321 cgctggcgtt cagctctccg gacgatctgc taagcaagtt gcaccccaac gaggacgggg
     7381 tgctgctgca tattggcccg cgcacggcga cgtttctgcc gcaggtgtgg gcgcagattc
     7441 ccgacaaggt caagttcctg gagtatctct cgcagaaagc cggcggtgaa gcctccgcct
     7501 ggcggggcaa agatgtctca gtcgccatct accatgttga gtgcttcgaa gaagagcact
     7561 cccgtccctg atcgccaggg gctggtcctg accgccgtcg gggcgctggg cgtctcgtgc
     7621 gtgatgacgc aactggcgct gatgcgcgaa ttgctggggg cgttcgcggg caatgagatg
     7681 gtgctgggcg tggtgctcgg cctgtggctg ttgctgatgg gcatcggcgc ctggctgggg
     7741 cacaactccg ataagctccg tcaccaactc accgcgctgg cattgatcca gatgctggtg
     7801 gcgctgctgc ccctcgccca ggtcttcctc ctgcgcgcgt tgcgcaacgt cgtgttcatc
     7861 cgcggtgcgg cggtgggcgt agccgagacg gtgggcgcgt cgtttgtgct gttgctgccg
     7921 tactgcctgg tcgcaggtta cgccttgacg ctggcgtgcg ggattctcgc gcgccagcaa
     7981 ggcccggccg gcatcggccg cgcctacgtg gccgacagca tcggcagcat aatcggcggc
     8041 gccttgttca gcttcgtgct agtccgctcc ttcgaccaca tcgccatcct cgcctatccc
     8101 gcgctgttga acctactgct ggccggcctg ttgggctcag gagttcaagg tttaaagtcc
     8161 aaagtccaaa gttcaaagtt cgcctccctc ctcctgcccg cgtccgccct gattctgggc
     8221 cttggcctca tcgccacaat ccttctgttc gacctggatg atctttccac ccggctgcaa
     8281 tacccgcggc agcacatcgt cgcccgggcc aactcgccct acggcaaact cattgtgacc
     8341 gagtcttacg gtcagtttga tttcctggag aacggcatcc cgattacctc gacgcgcgac
     8401 gacgagcacg tcgaggagac cgtgcattag gcgatggctc agcggcccga ggcgcggcgg
     8461 gtgctgctgg tgtctggcgg cgtctccggc acggccaggg agattctcaa atacggtgtc
     8521 cccgcggttg actacgtgga actcgacccc ctgatcctgg tgctgggccg acgctatgtg
     8581 ccggcgaacc tcgcggatgg acgaatccga gtcatcaaca ccgatggacg cctgttcgtg
     8641 aagcagaccc cggagaaata cgatgtggtc atcattgatg ttcccgcccc ggccacggca
     8701 cagcttaacc gcttctatac cgtggagttt ctggccgagg ttaagcgggt gctggccagg
     8761 gacggcgtgg tgggcttcgc gctcggccag tatgagaatt acgtcagccc ggaactcgcc
     8821 cggatgttgt cctccgcctg tcttagtgcg aaacagtcat ttcgcaacgt gctggctatc
     8881 cccggcgggc gggtgttctt cctggcctca gacgggccgc tgtatgcgaa tatcgctgca
     8941 cgcatcgagc agcgccacgt caagaccaag ttggtcaacc gccactacct ggacggcacg
     9001 gcttcggcgt ttagaaagcc gtttgtctgg tgagactggg gcgtgagcgc agcaaggagt
     9061 tcaactggaa agtatatcgc cgagggtaag ttgacgggct gccataggaa ggatctggtc
     9121 agattcgggc agtcatg
//
LOCUS       DATCGS010000422         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_135699, whole genome shotgun sequence.
ACCESSION   DATCGS010000422 DATCGS010000000
VERSION     DATCGS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135699"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VIL39_08695"
     CDS             <1..222
                     /locus_tag="VIL39_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5481541.1"
                     /translation="VAPAECSASAQAKKMDRVTADGFAVHSFDTLLRELATRCRNTCR
                     IPSEASGATFQQLTEATPLQARALQLLGL"
     gene            529..2406
                     /locus_tag="VIL39_08700"
     CDS             529..2406
                     /locus_tag="VIL39_08700"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEY5481542.1"
                     /translation="MYIRKASRTVHGKTYFNYLLVESHLTSKGPRQKVICSLGDLSPR
                     PKEQWLELARKLENALTGQPDLFASASPDPELETLVAKVKAGPRRRRAQQDQQALATS
                     VEPGEEKIAVLTGAVRSEQSREAGPVHVGYQFWQRLGINAILQEAGLGERARQLTCAM
                     TLNRLIHPASELAMPEWIRTTALPDILGVDFQDLAEDALYRNLDKLHPRRNQIEAALA
                     ERERNLFDLDQTVFLYDVTSTYFEGRALANPKAQRGYSRDHRGDCKQVLVGLAVNRDG
                     FPLAHEVMAGNRHDSTTLEEMLGALDQRVGLRSGQTVVVDRGMSGEENLEKIRARGLH
                     YLVAAPYSERHEWVEEFQDAAEFEEVRREPSPRNPFQKKSTIQVKLRKAGGETHVLCV
                     SSERKEKDRAIRQAHEKRLVADLAKLEKRVAKGKGKGTKPEEVQQSIGRLRERYSRVA
                     RYYRMEYDAQAKTFRYSLDEAKRAQAEKLDGSYLLKTDREDLSADEAWRIYILLTRAE
                     AAFRTLKSPLGERPVFHHKEGRVEAHIFLCVLAYHLLISIEKTLVNEGVHTSWATVRE
                     TLKTHQVNTIVLPTDGGLVLRIRKGTPPEPAHRELYAKLGIEPEVIRPRKSWTRAEIS
                     K"
BASE COUNT          507 a          744 c          800 g          424 t
ORIGIN      
        1 gtggcgcccg ccgaatgctc cgcttccgcg caggctaaga agatggatcg cgttaccgcc
       61 gacggctttg cggtccacag cttcgacacc ctgctgcgcg aactcgccac gcgctgccgc
      121 aacacctgcc gcatcccctc cgaggcgagc ggtgccacgt tccagcaact gactgaggcg
      181 accccgctcc aagcgcgcgc actgcaactc ctcggcctgt agccagaaac cagaaccccg
      241 ccagacaccc ttcccgaatc aaatcatccg gatagtcccc attgcggcat cgaacttcgg
      301 cttagcccaa cttccgcacc accactgacc ggtgaggtcg gttttcgggc ggtttcgatt
      361 caggctgcga ttcaactcct tcgttttcag tcatgaggtc tggagagcgc tgaaatagtg
      421 actactagta gggacaatag attgccgaca gtctcgatcc aatatagata agcattgctt
      481 atttatcttg actctttctc ccttctgttt tttaatagtg gctatagtat gtatatccgc
      541 aaagcttcgc ggacggtcca cggcaagacc tacttcaact acctccttgt cgaaagccac
      601 ctcacttcca aaggcccccg ccagaaggtc atctgctcgc tcggcgacct ctctccgcgt
      661 cccaaggagc aatggctcga gctggcccgc aagctggaga acgccctcac cggccagccg
      721 gatctgttcg cctccgccag ccccgatcct gaattggaaa cgctcgtcgc caaggtgaag
      781 gccgggcccc gccggcgccg cgcccagcag gatcaacagg cgctggccac cagcgtcgaa
      841 ccgggcgagg agaagatcgc ggtcttgacc ggcgccgtgc gcagcgagca atcgcgcgag
      901 gctggaccgg tccacgtggg ctatcagttc tggcagcggc tcgggatcaa cgcgattctg
      961 caggaagcgg gcctgggtga gcgggcacgt cagttgacct gcgcgatgac gctcaaccgc
     1021 ttgatccatc ccgccagtga gctggccatg ccggagtgga ttcgcaccac ggcgctgccc
     1081 gacattctgg gggttgactt tcaggacttg gccgaagacg cgctgtatcg caatctcgac
     1141 aagctgcatc cgcgccgcaa ccagatcgaa gcggctctgg ccgagcgcga gcggaacctg
     1201 tttgacctgg atcaaacggt gtttctctac gacgtcactt caacctactt tgagggccgt
     1261 gcgctggcca acccgaaagc ccagcggggg tactcgcggg accatcgcgg cgactgcaaa
     1321 caggtgctgg tgggactggc ggtgaaccgg gacgggtttc cgctggcgca cgaagtgatg
     1381 gccgggaacc ggcacgactc gacgacgctg gaggagatgc tcggcgcgct cgaccagcgg
     1441 gtggggctga gaagcggcca gacggtggtg gtggaccgag gcatgtcggg cgaggagaat
     1501 ctggagaaga tccgggcgcg aggcctgcac tatctggtgg ccgcgcccta cagcgagcgg
     1561 cacgaatggg tggaggagtt tcaagacgcg gcggagtttg aagaagtgcg gcgggagccg
     1621 tcgccgcgca acccgttcca gaagaagtcg acgattcagg tgaagctgcg caaagcgggc
     1681 ggcgagacgc acgtgctgtg tgtgagcagc gagcggaaag aaaaggaccg cgcgatccgg
     1741 caggcgcacg agaagcggct ggtggcggac ttggcgaaac tggaaaagcg cgtggccaag
     1801 ggcaagggga aagggacgaa gccggaagag gtgcagcagt ccatcgggcg gctcagggag
     1861 cgttactcgc gggtggcgcg ttactaccgg atggagtacg acgcccaggc caagacgttt
     1921 cgttacagtc tcgacgaggc caagcgggcg caggccgaga agctcgacgg gagttactta
     1981 ctgaagaccg accgcgagga tctgagcgcc gacgaggcct ggcgaatcta cattctgctg
     2041 acgcgggcgg aggcggcgtt tcgcacgctc aagtcgccgc tgggcgagcg gccggttttt
     2101 catcacaagg aaggccgcgt ggaagcgcac atattcctgt gcgtgctggc gtatcatctg
     2161 ctgatctcca tcgagaagac gctcgtcaac gagggcgtcc acacgtcctg ggcgacggtg
     2221 cgcgagacgc tgaagacgca ccaggtgaac acaatcgtgt tgccgaccga cggcggcctg
     2281 gtgctgcgca tccgcaaggg caccccgcct gagccggcgc atcgcgaact gtatgcgaag
     2341 ctgggcatcg agccggaggt catccggcca cgcaagagtt ggacgcgggc ggagatctca
     2401 aaatagtgac tgaaaaaccg acaagtcctt gtttttcaat ggtccctttt caaaagcgcg
     2461 gaagttgggc tagct
//
LOCUS       DATCGS010000423         8868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_431475, whole genome shotgun sequence.
ACCESSION   DATCGS010000423 DATCGS010000000
VERSION     DATCGS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431475"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VIL39_08705"
     CDS             complement(<1..349)
                     /locus_tag="VIL39_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481543.1"
                     /translation="MMSKQEMLGAMCLELGQADLKAMGQSRGFDPQTIASRKLLEHVF
                     LSEQGLPAALASLTEAETLGLHLLNCLREAVDLDFFKPVYPGLVPGGYYGSYTESHKG
                     LFQKVKTQLVRRGI"
     gene            736..2178
                     /locus_tag="VIL39_08710"
     CDS             736..2178
                     /locus_tag="VIL39_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEY5481544.1"
                     /translation="MNNLPLLPPIICLLFSIVPVWAGSVQVYPAPAGEELSKDFDVQV
                     EGKQIPVYVAKVASAEPERRWKAMDDKAHSAEYFEKASFAYFDMQGTVTVTVSCRQVI
                     QSAKVLPSSWKITPTIQGKRLSLTLSEAKPLTIEVNGNWVGSLHLFANPPEAQAPKPA
                     DPNVVFFGPGIHEVSHLVVTNNQTVYVAGGAIVRAIIGSEEPFQISSYSGLRTYSPTF
                     VLRGTNITFRGRGIIDGSRCTTHARNLVQVQGQDITLEGVILRDSSTWNIPIRRSERV
                     TVKNLKLLGYRANSDGIDICSSRDVTVESCFLRTLDDLIVVKTDKGQGEARRIVARDC
                     VLWNEVAHALSIGAELRENVDDVLFTNCDIIHDKGREWTLRVYHCDSARISNVRFENL
                     RIEESPRLISLWIDKASWTRDSERGHIDGVAFKNIRAVADPLRVELHGFDETHAVENV
                     VFEGVVVNGKPLSPADVKTNAFVRNVDYRR"
     gene            2323..3282
                     /locus_tag="VIL39_08715"
     CDS             2323..3282
                     /locus_tag="VIL39_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5481545.1"
                     /translation="MKTAVVCGAGGFIGSHLVKRLKREGYWVRGVDIKPTEFAPSAAD
                     EFLLLDLREEAACHAAVKIKGKPADEVYQLAADMGGMGFIHSAECEIMHNSALINIHM
                     THCAATAGVPRYFFSSSVCVYRDMKLGEPEMKEAEAVPANPDNEYGWEKLYSERVAQA
                     NGRRHGMAVRIARFQNCYGPEGTWTGGREKAPAAICRKVAQAADGGVVEMWGDGSAVR
                     SYTYVDDMVDGIFRLMHSDLEGAANIGCPQYVTVRELTELVAQIAGKRIQIKPVPGPV
                     GVHSRNFSNERIYTTGWKSRFDLKAGLSLTYPWIKQQVDAAKK"
     gene            complement(3344..4480)
                     /locus_tag="VIL39_08720"
     CDS             complement(3344..4480)
                     /locus_tag="VIL39_08720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5481546.1"
                     /translation="MRFLMLNWRDPMNPESGGAERVSQAYLGALVERGHEVWWFANEF
                     PGGERAETLRGMKIVRGGGKGTSIVKAIRWYRQQPRFDLVMDQHHGIPWYAPWWCGTN
                     HIAYIHEVLGPIWNTFYSWPLSWLGRRQERWTHWLYRRVPFWTACESTVACLRRHGVN
                     HVTIIRYGVNTQALPELDPKPLGPPLKLAVVSRLAPNKRIAECLRTLAVLRQRGVAAT
                     LQVVGSGACEAALQQEAAQLQLTDSVTFTGPLPEAEKDAILRNAHFLLHTSVREGWGL
                     NVIEANAMGTPAAVYPVEGLIESTLHDRTGLVAAAETPQALADQIVGVLADDARYQRY
                     RRAAWERAKTLHWSQVLPRACDWLEARAKGAPLTGESGQIAAGG"
     gene            complement(4594..5022)
                     /locus_tag="VIL39_08725"
     CDS             complement(4594..5022)
                     /locus_tag="VIL39_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY5481547.1"
                     /translation="MRASGRRLVVTNGCFDLLHLGHVTYLETARQQGDALLVGVNGDE
                     AVRQLKGPDRPVNTEHDRAAVLAALESVSGVCIFAEATATRFLAAARPDVYVKGGDYT
                     LDTLNQEERRTVEQAGGKIVIIPFVPGKSTTALLKKISRL"
     gene            complement(5197..5985)
                     /locus_tag="VIL39_08730"
     CDS             complement(5197..5985)
                     /locus_tag="VIL39_08730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5481548.1"
                     /translation="MANTFFDPGDQRAAKVQDLFARIAPRYDLINDLQSFGLHRYWKR
                     RVVRLACPQAGQRALDVCCGTGDLALALARRGAHVAALDFSERMLDAAQRRQSRLQGQ
                     GPGGSDHASRIPHPASPPSRFVQGDALRLPFSDNSFDIVTVGYGLRNLANWETGLHEM
                     LRVANPGGRVLALEFGKPDNALWRGLYFGYLKLFVPCLGRIFCGNTGAYAYILESLKH
                     YPAQHAVAAKMRELGLVNVRTVGLLGGAMSINYGEKRSNGVLEY"
     gene            complement(6008..6820)
                     /locus_tag="VIL39_08735"
     CDS             complement(6008..6820)
                     /locus_tag="VIL39_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5481549.1"
                     /translation="MKFAICNEIFQGWKLEDTLACAARLGYAGVEIAPFTLANSVTDI
                     SATERQAMREAAARNRIEIAGIHWVLVKPEGLYINHPDCGLRERTAKYFCDLVDFCAD
                     LGGKVMVVGSPKQRNVLPDVSREQAWDWAATTFRNAVRQAEQREVTICFEPLAPVETN
                     FVNTAAEALQFVQQVASPRFKIILDVKAMCSESKPIPQIIRESWPHFAHFHANDRNLK
                     GPGFGDVDFKPIAAALKEVGYQGFVSVEVFNFDEGAEAIASRSLEYLRRAFA"
     gene            6973..8122
                     /gene="prfB"
                     /locus_tag="VIL39_08740"
     CDS             join(6973..7041,7043..8122)
                     /gene="prfB"
                     /locus_tag="VIL39_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_163967019.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5481550.1"
                     /translation="MFDNITAELATATDKLAHLRRFLDVPGAQRRLGEVNALMAAENF
                     WNNSEQARKLIDEANSLRNKTEPLLKAEKQLEDFHIMVELGEAEPEVAQLKIQQELEQ
                     DLSRFFKELDSLELKVFLNGRHDKSNCIMNINAGAGGTESCDWASMLLRMYQRWAEGR
                     GWQVEVNDVLPGETAGIKSATIVMTGENAYGYAKAERGVHRLVRISPFDSNKRRHTSF
                     ASVDVIAEIEENDEEIAIPPNEFAVDTFRSGGKGGQNVNKVETAVRITHIPTGLIVAS
                     QSQRSQHQNRAAAMKLLLSRIYAQRVDAQKAEMERFYGDKGSISWGNQIRSYVFQPYR
                     MVKDLRTGVETSNVQAVMDGDLDQFVNGWLRAGCPLKRMQGVRDEEEG"
     gene            8268..>8868
                     /locus_tag="VIL39_08745"
     CDS             8268..>8868
                     /locus_tag="VIL39_08745"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY5481551.1"
                     /translation="MTILDTIVEEKKREVARLPQRSLSIGDLQAALDARGGRRDFAGA
                     LRKPKIGSVALIAEVKKASPSAGVIRPDFDPVRIAREYEAAGASCLSVLTDQKFFQGS
                     LDYLKEIRGAVGLPLLRKDFIVDERQILEAIEWGADAVLLIVAILSDAQLKHFHALAT
                     GAGLAALVEVHDEADLDRALNAGAELIGVNNRNLKTFKVD"
BASE COUNT         1812 a         2604 c         2731 g         1721 t
ORIGIN      
        1 atatcccccg gcgaaccaac tgcgttttga ccttttgaaa gagcccctta tgactctcgg
       61 tgtagcttcc ataatagccg ccgggaacca gcccgggata aaccggtttg aaaaagtcca
      121 agtccaccgc ttcccgcagg cagtttaaca agtgcagccc gagcgtctcc gcttcggtca
      181 gcgacgccag cgccgctggc agcccctgct ccgaaaggaa aacatgctcc agcagcttcc
      241 gcgaagcaat ggtctgggga tcgaaccctc ggctctggcc catggccttg aggtccgcct
      301 gacccagctc caggcacatt gccccaagca tttcttgctt ggacatcatg atgacgcgtc
      361 tttcatggcg cgtggatatg tggcagattt gcggcggcct gccaactctc gatttctgcc
      421 gaatcactga gcagctaaca ggaaaagcgc acccattagc gtgagcggtt aatgagattg
      481 tcactcaccg cacaggggca agacgggcga gcgggtctcg ccggcttcct caaccgggaa
      541 ttgagaggaa tagacgcaac ctctgcctcg gctttgtgat catcgtaggg cgctggccga
      601 atcttgatga aagagatagt tttggctggc attgtgcggt ccttgccttt cattatccgc
      661 caatgccagc ttacattatt gcccggggga tgggccattt gaaatcccat cggccagcaa
      721 ccacgcggat gccccatgaa taacctcccc ctgctgcccc caatcatttg cctcctgttc
      781 tcgatcgttc cggtttgggc tggctccgtt caggtttatc cagcaccagc aggcgaggag
      841 ctttcaaagg attttgatgt gcaagtcgag gggaagcaga ttcctgttta tgtcgccaag
      901 gtcgctagcg ctgagccaga gcgccggtgg aaggcgatgg atgacaaagc ccattcggct
      961 gagtatttcg aaaaggcctc ctttgcctat ttcgatatgc agggcaccgt gaccgttacc
     1021 gtgagctgtc gccaggtgat acagtcggcc aaggtgcttc cctcctcttg gaagatcact
     1081 ccgaccattc aggggaaacg cctgagctta accctctccg aagccaagcc cttgaccatc
     1141 gaggtaaacg gcaactgggt tgggtcgttg caccttttcg ccaatccacc cgaagcacag
     1201 gcgcccaagc cggccgatcc gaatgtcgtg ttcttcggcc ccggcattca cgaggtcagc
     1261 cacctggtcg tgaccaacaa ccagacggtt tatgtcgccg gaggagcgat tgtgcgagcc
     1321 atcattgggt cggaggagcc cttccagatt agcagctata gcgggctgcg cacttactct
     1381 cccaccttcg tgctcagagg gaccaatatt acttttcggg gccgagggat tattgatggg
     1441 agccgttgca caactcacgc tcgaaatctg gtccaggtgc agggccagga catcaccttg
     1501 gaaggagtca ttctgcgcga ttcaagcacc tggaacattc ccatccggcg gtcggaacgg
     1561 gtcaccgtca agaacctgaa gttgttgggc taccgagcca actcggatgg cattgacatt
     1621 tgcagcagcc gggacgtgac ggtcgaaagt tgctttttac ggaccctcga tgatctgatt
     1681 gtggtcaaaa cggacaaagg ccaaggggag gcgcggcgca tcgttgccag ggattgtgtc
     1741 ctctggaacg aggtagcaca cgcattgagt atcggagccg aacttcggga gaacgtggac
     1801 gacgtgctct ttaccaactg cgacatcatc cacgacaaag gccgcgagtg gacgctccga
     1861 gtatatcatt gtgattctgc ccgcatcagc aatgtgcggt tcgaaaacct gcgcatcgaa
     1921 gaatctccgc ggttgatttc tctttggatt gacaaagcgt cttggactcg ggacagcgag
     1981 cggggccaca ttgatggggt tgcttttaag aacattcggg cggtggctga tcccctgcgt
     2041 gtggaactcc atgggttcga tgagacgcat gcggtagaga atgttgtgtt tgaaggcgta
     2101 gtcgtgaatg gaaagccact ctcgccggct gatgtgaaaa cgaatgcttt tgtcagaaac
     2161 gtagattacc ggcgctgatt cgaagtcagc gcaggtcatc gaagcgttga tctcaatggt
     2221 cagggatttc cagacggcgc tcaaacgcca cgaagaggcc gtgtcaggga gagcttgcaa
     2281 ttcgaaccta atccggtagc ttttgtgctt ctgtaagatt agatgaaaac tgctgttgta
     2341 tgcggagccg gtggattcat cggcagtcac ttggtcaaac ggctcaagcg tgaaggctat
     2401 tgggtccgcg gcgtggacat caagcccacc gaattcgccc ctagcgccgc cgacgaattc
     2461 ctgctgctgg acctgcgcga ggaagctgcc tgccatgccg ccgtcaagat caagggcaag
     2521 cccgccgacg aggtctatca actcgcggcg gacatgggcg ggatggggtt catccattcg
     2581 gccgagtgcg agatcatgca caacagcgcg ctgatcaaca tccacatgac ccactgcgcc
     2641 gccacggctg gcgtgccgcg gtacttcttt tcctccagcg tctgcgttta tcgcgacatg
     2701 aagctgggcg agcccgagat gaaggaagcg gaagccgtcc ccgcaaaccc cgacaacgaa
     2761 tacggttggg aaaagctcta ttccgagcgc gtggcgcagg ccaatggccg ccgtcacggc
     2821 atggccgtgc gcatcgcccg tttccagaac tgctacggcc cagagggtac ctggaccggc
     2881 gggcgggaga aagcgcccgc cgccatctgc cgcaaggtcg cccaggccgc cgacggtggc
     2941 gtggtggaga tgtggggcga tggctccgcg gtgcgttcct acacctacgt ggatgacatg
     3001 gtggatggca tctttcggct gatgcactcc gacctcgagg gtgccgccaa catcggctgc
     3061 ccgcagtacg tcaccgtgcg cgagttgacg gaactcgtcg cccagattgc gggcaagcgc
     3121 atccagatta agcctgtgcc cggcccggtg ggcgtgcatt cccggaattt cagcaacgaa
     3181 cgcatctaca ccaccggctg gaaatcgcga tttgacctga aggccggcct gagcttgacc
     3241 tacccgtgga tcaagcagca ggtggacgcc gcgaagaagt gatcggcgct ggccgcggat
     3301 atttcatacc cgcggggccg gagcgccggc ttcgggaagc agctcagcca ccggcggcta
     3361 tctgaccgct ttcccctgtc agcggcgcgc ctttggcacg ggcctccagc caatcgcacg
     3421 cgcgcggcag cacctggctc cagtgcagcg tcttggcccg ctcccacgcc gcgcggcggt
     3481 aacgctggta acgggcgtcg tccgccagaa cgccgacgat ctggtcggcc agcgcctgcg
     3541 gagtttcggc tgccgcgacc aggccggtac ggtcgtgcag cgtggactca atcaaccctt
     3601 ccactggata aaccgcggcc ggcgtgccca tggcattggc ctcgatcacg ttcagtcccc
     3661 agccttcacg gacagaggta tgcagcagga aatgagcgtt ccgcaggatg gcgtccttct
     3721 ccgcttccgg cagcgggccg gtgaacgtga ctgagtccgt caactgaagt tgggcggctt
     3781 cctgctggag cgcggcctcg cacgcgccgc tacccaccac ctgcaaggtc gcggccacgc
     3841 ctcgctggcg caggaccgcc agggtgcgca ggcattcggc aatgcgcttg ttcggcgcca
     3901 ggcgtgagac caccgcgagt ttgagcggtg gtccgagggg tttgggatcc agctcgggca
     3961 gggcctgggt attgaccccg taacggatga tcgtgacgtg gttcacgccg tgacggcgca
     4021 ggcaggccac ggtagattca caggccgtcc agaagggcac gcgccggtaa agccaatgcg
     4081 tccaacgctc ctgccggcgg ccgagccagc ttaaaggcca agagtaaaag gtgttccaga
     4141 tcggaccgag cacttcgtgg atgtaagcga tgtggttcgt cccgcaccac cacggcgcgt
     4201 accaggggat cccgtggtgc tggtccatga cgagatcgaa ccgcggctgc tggcgatacc
     4261 agcggatggc cttcacgatg gacgtgccct tgccaccgcc tcgcacgatc ttcatccccc
     4321 gaagggtttc cgcacgttca ccgcccggga attcattcgc gaaccaccac acctcatgcc
     4381 ctctttccac caacgctccc agataagcct ggctgacccg ttcggcaccc cctgactccg
     4441 ggttcattgg atcgcgccag ttgagcatca ggaaacgcat gagttatggc gggtgtttat
     4501 ggacaaaacg tgtccccgag acaaccataa agtgccttac tctgccgcca agccactgat
     4561 caccacaagg ccagccggca gccggaatcg aggtcatagt cgcgagatct tcttcagcaa
     4621 cgccgttgtg gacttgccgg gcacgaaggg aataatcact atcttcccgc cagcttgctc
     4681 gacggtgcgg cgttcttcct ggttgagggt gtccagggtg taatcgccgc ctttgacgta
     4741 aacatccggt cgcgcggcgg ccaggaaccg cgtggcggtt gcttcggcga agatgcaaac
     4801 tccgctcacg ctttccagcg cggccagcac ggcggcgcgg tcgtgttccg tgtttacggg
     4861 gcggtccggt ccttttaact ggcgcacggc ctcatcgcca ttaacgccca ccagcagcgc
     4921 gtcgccctgt tgccgcgccg tttccaggta agtcacatgg ccgaggtgga gcaggtcgaa
     4981 gcagccgttg gtgaccacca gccgtctgcc gctcgcacgc atggctgcac gccacgccgg
     5041 caatgtttcc cagataagga tcttgtcacg gaagttcacc ggggcattct ggccaaagtg
     5101 cttccgcctt ggcaaggaag gaggcgaggg gagggggaat ggagtaatgg agtgatggag
     5161 tgatggagtg gtggagggat ggaaacgagc cggttctcaa tactccaata ctccattact
     5221 ccgtttctcc ccatagttga tgctcatcgc gcctcccagc aggccaacgg ttctcacgtt
     5281 gaccagaccg agttctcgca tcttcgccgc aaccgcatgc tgggccgggt agtgcttgag
     5341 tgattcgagg atgtaggcat acgcgccggt gttgccgcag aagatccgcc ccaggcaggg
     5401 cacaaacaac ttcaggtagc cgaagtagag gccgcgccag agcgcgttgt ccggcttgcc
     5461 gaattccagc gccagcactc gaccgccggg attggcgacc cgcagcattt cgtgtaagcc
     5521 ggtttcccag ttggccagat tgcgcaaacc atatcccacg gtgacgatgt cgaaggagtt
     5581 atcggaaaac ggaagacgca gcgcgtcgcc ttggacgaag cgcgagggag gtgatgcggg
     5641 atgcgggatg cgggatgcgt gatccgaacc tcccggccct tgtccttgga gtctggattg
     5701 cctcctctga gcggcgtcca gcatccgctc gctgaaatcc agggccgcca cgtgagcgcc
     5761 gcgccgggcg agcgccagcg cgagatcgcc cgtgccacag caaacgtcca acgctcgctg
     5821 cccggcttgc gggcaggcca accggaccac gcgccgcttc caataccggt gcagcccgaa
     5881 gctctggagg tcattgatca ggtcgtaacg cggcgcgatc ctggcaaaga gatcctgcac
     5941 cttcgcggcg cgttggtcgc ctgggtcgaa aaatgtgtta gccatggctg aacaagtctc
     6001 cggcaaatca agcaaacgca cgcctcaaat actccaggct ccggctggca attgcctccg
     6061 cgccttcgtc gaagttgaac acttccaccg aaacgaagcc ctggtagccg acttccttta
     6121 gtgcggcagc gatcggtttg aaatcaacat caccaaagcc gggtcctttc agattccggt
     6181 cgttcgcgtg gaagtgggcg aagtgcggcc acgattcacg gatgatctgg ggaatgggtt
     6241 tggattccga gcacattgcc tttacgtcga ggatgatctt gaaacgaggc gacgccactt
     6301 gctggacgaa ctgaagggct tcggcggcgg tgttgacgaa attcgtctcg accggcgcca
     6361 ggggctcgaa gcaaatggtg acctcccgtt gctctgcttg tctgaccgcg tttcgaaacg
     6421 tggtcgccgc ccagtcccac gcctgctcgc gggaaacgtc cggcagcaca ttgcgctgct
     6481 ttggcgagcc gacgaccatc accttgccgc cgagatcggc gcagaaatcc accaggtcgc
     6541 agaaatactt cgccgtgcgc tcgcgaaggc cgcagtccgg gtgattgatg tagagtcctt
     6601 cgggtttcac cagaacccaa tggatgccgg cgatctcgat ccggttacgc gccgcagcct
     6661 cgcgcatggc ctggcgctcc gtggcggaaa tgtctgtcac tgaattcgcc aaagtgaacg
     6721 gggcgatctc gactccggca taaccgagtc gcgcggcaca agccagggtg tcttccagct
     6781 tccagccttg gaagatctca ttgcaaatgg caaatttcat ccgcgccaga ctcaagcttt
     6841 gcgcttcacg attcaagatt gaaacggcgg tgtcccgtcg gcggtacaga ccacctgcgc
     6901 caggacagtc ggacggtcgc gctcttcacc ttcaacttgc tacttggccc cgcgcgacct
     6961 accttacaag ggatgtttga caatattaca gccgagctgg ccaccgcgac cgataaactg
     7021 gcccatctgc ggaggtttct ttgacgtgcc gggtgcgcag agacgcctgg gcgaagtcaa
     7081 cgccctgatg gcggctgaga atttctggaa caacagcgag caggcccgga agctcataga
     7141 cgaggccaac tcgctccgca acaagactga accgctgctc aaggcggaga agcagctcga
     7201 agatttccat atcatggtag agctgggtga agcggagccg gaagtcgccc aacttaagat
     7261 ccagcaggag ctggaacagg atctctccag gttcttcaag gaactcgatt cgctcgaatt
     7321 gaaggtcttt ctcaacggcc gccatgataa gagcaattgc atcatgaaca tcaacgcggg
     7381 tgcggggggc accgaatcgt gcgattgggc aagcatgctc ctgcgcatgt accaacgctg
     7441 ggccgagggg cgcggctggc aagtggaggt caacgacgtg ctgccgggcg aaaccgccgg
     7501 cattaagagc gcgacgatag tcatgaccgg tgagaatgcc tatggctatg ccaaggctga
     7561 gcgcggcgtg catcggctgg tgcgcatctc gccgtttgac tccaacaagc gccggcacac
     7621 cagtttcgcc agcgtggacg tgatcgcgga aattgaggag aacgacgagg agatcgcgat
     7681 tcctcctaac gagtttgcgg tggacacctt tcggtccggc ggtaaaggcg gccagaacgt
     7741 gaacaaggtc gaaaccgccg ttcgcatcac gcacattccg acggggctga ttgtcgcctc
     7801 gcagagccaa cgttcccaac atcagaaccg cgccgcggcg atgaagctcc tgctctcgcg
     7861 catctacgcc cagcgcgtgg acgcgcaaaa agccgaaatg gagcgcttct acggtgacaa
     7921 aggcagcatc tcctggggca accagattcg cagctacgtc ttccagccgt atcggatggt
     7981 gaaggacctg cgcaccggag ttgaaaccag caacgtgcag gcggtgatgg acggcgatct
     8041 ggaccaattc gtaaacggct ggctccgcgc cggttgtccg ctcaaacgga tgcagggggt
     8101 gcgggatgag gaggaagggt gaaatccgaa ggtcgaaggc cgaagaccga aggaaggccg
     8161 aagcccgaaa tccgaatcat gtgcgtgggg ctagagctac tagcccatgt agtcgcgggt
     8221 gattcgagca tgcggtttcc ggtttctgac tacacgcaga caggtctgtg actattcttg
     8281 ataccatagt tgaagagaag aagcgcgagg tggcgcggct gccgcagcgc tcgctctcga
     8341 ttggcgactt gcaggcggct ctcgatgcgc ggggagggcg gcgggatttt gctggcgcgc
     8401 tgcggaaacc gaagatcggt tcggtcgcct tgattgccga ggtcaaaaag gcatcaccgt
     8461 ctgccggggt gattcggccg gacttcgatc cagtccggat cgctcgggaa tacgaagcgg
     8521 cgggcgcaag ctgtctgtcg gtgctcacgg atcagaagtt cttccaaggg tcgctggatt
     8581 acttgaagga aatccgcgga gcggtgggac tgccgctgct gcggaaggat ttcatcgttg
     8641 atgagcggca gattctggag gcgattgagt ggggcgccga cgcggttctg ctgattgtcg
     8701 ctatcttgag cgatgcgcag ttgaagcatt tccatgcgct ggcgacggga gccggcctgg
     8761 cggcgctggt cgaggtgcat gacgaagctg atctggatcg cgccctgaac gcgggagcgg
     8821 aactgatcgg cgtgaacaac cgcaacctga agaccttcaa agtggacc
//
LOCUS       DATCGS010000424         3217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_532176, whole genome shotgun sequence.
ACCESSION   DATCGS010000424 DATCGS010000000
VERSION     DATCGS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532176"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VIL39_08750"
     CDS             complement(<1..242)
                     /locus_tag="VIL39_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481552.1"
                     /translation="MKVARIATEVNCQDRKRQDHKRSKTPACIGITKGYSLRRMRPMT
                     TVLVLAALAICPCARAARVTATAQAPAKTYRNPLLP"
     gene            288..1472
                     /locus_tag="VIL39_08755"
     CDS             288..1472
                     /locus_tag="VIL39_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5481553.1"
                     /translation="MPAALEMLRQMVGINSFTGNRDGVNRLSRFTGECFAPLGFTAEY
                     VSSTNRDWGDHLVLTRRGRSDRSIALISHLDTVFPPEEEARNNFRWLVEGDRIFGPGT
                     HDIKGGSVMMWLLLVALRSQAPKLFEDITWKLIWNSSEECYSPDFGDVCRARFDRSTL
                     AALVFESEGRAAKESLLVVARKGRGTWRVTASGRGSHAGGKHEHGANAIVQLGQTVQR
                     IAALTDYGRGLTVNVGTIRGGTVLNRVPHEAVAEGEFRTFTPEAFAHAKAGLLALAGR
                     GDVRSPADGYACEVKVEILTESRPWPRNPGTDGLYQLWKQAADELGLAVNCQERGGLS
                     DGNLIWDAVPTLDGLGPWGDNDHCSERSLDGAKLPEFVDASTFVPKAMLNIAAIQKLY
                     GA"
     gene            1556..2119
                     /locus_tag="VIL39_08760"
     CDS             1556..2119
                     /locus_tag="VIL39_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481554.1"
                     /translation="MTLKLSTLSILLGLGMGLPQIYGIAKPAAFAAAVRKFPRSLPWG
                     IALMVLGTVWFLWNLSQESIADFASYKNVLLAGFAGVGLGACIFVQDFLAVRGLAVVL
                     LLLAKLMVDTGRPSLPQTHWVIVIQTWGYVLVVAGMWLTVSPWRLRDWLTWGTANNNR
                     VKVVCGLKLAFGLFVAWLGLTVFKPGV"
     gene            2153..3106
                     /locus_tag="VIL39_08765"
     CDS             2153..3106
                     /locus_tag="VIL39_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY5481555.1"
                     /translation="MNSLRGRILVIRGGAIGDFILTLPAIAALRRQFPQAHLEVLGYP
                     HIAQLALVGGLVNRVQSIEAGALAGFFARGGELAENLADYFSEFDLILSYLYDPDAIF
                     QTNVGRCTGAQFIVGPHRVDEAARLHAAKVFLQPLERLAIFDADPIPRLPPPSLNSQL
                     STLNLLALHPGSGSERKNWPEAKWAELLQYLVSSTEFDLLLVGGEAEGDRLQRLAAAL
                     PPARSQVAQSVPLAELAGRLAACRAFVGHDSGISHLAAAVGLPGLLLWGDTAEEIWRP
                     PSEKMRVLRHPVGLDRLPVAEVMEQLRRVLVASCGLTPGLR"
BASE COUNT          539 a          995 c         1044 g          639 t
ORIGIN      
        1 tccggcagca gcggattgcg ataggtttta gcgggagcct gggcagtggc agtcacccgc
       61 gccgcgcggg cacaggggca gattgccagc gcggccagaa ccagaacagt tgtcattggt
      121 ctcatgcggc gcagactata gcctttcgtg atgccgatgc acgccggagt tttggagcgc
      181 ttgtggtcct ggcgcttgcg gtcctggcag ttgacctccg ttgcgattct cgccaccttc
      241 acgccatgca gaacgctgga ccgttgaacg catttcttag aagccaaatg cccgctgccc
      301 tggagatgct gcggcagatg gtcgggatca acagttttac cgggaaccgc gacggggtga
      361 accggctcag ccgtttcacc ggcgagtgct ttgcgccact cgggttcacc gctgaatatg
      421 tttcctcaac caatagggac tggggcgacc atttggtgct gacgcggcgc gggcggtcgg
      481 accggagtat tgccttgatc tcccacttgg acaccgtctt tccgcccgag gaagaggcgc
      541 gcaacaactt ccggtggctg gtggagggcg atcgcatctt cggcccgggc actcacgaca
      601 tcaaaggcgg gtcggtaatg atgtggctcc ttttggtggc gctccggtcc caggcgccaa
      661 aactcttcga ggacatcacg tggaagctca tctggaactc ctccgaggag tgctactcgc
      721 cggacttcgg cgacgtctgc cgcgcccgct tcgaccgcag cacactggcc gcgctggtct
      781 tcgagtccga ggggcgcgcg gccaaggaga gcctgttggt cgtggcgcgc aaagggcggg
      841 gcacctggcg agtcacggcc agcggccgcg gttcgcacgc ggggggaaaa cacgaacacg
      901 gcgccaatgc catcgttcaa ctcggtcaga cggtgcagcg catcgcggcc ctcacggact
      961 atgggcgcgg cctcaccgtg aacgtcggca cgattcgcgg cggcaccgtc ttgaaccgcg
     1021 tcccacatga ggcggtcgcg gaaggcgaat tccgcacctt cacgcccgaa gcctttgccc
     1081 acgccaaagc cggcctgctc gccctcgcag ggcggggcga cgtgcgcagt cccgcggacg
     1141 gctatgcctg tgaggtgaag gtcgagattc tcaccgagtc ccgcccctgg ccgcgcaacc
     1201 cgggcaccga cgggctctac cagctttgga agcaagccgc ggacgaactc ggcttggcgg
     1261 tcaactgcca ggagcgcggc ggcctgagcg acggcaacct catctgggac gcggtgccca
     1321 ccctcgatgg tctgggcccc tggggcgaca atgaccattg ctccgagcgc agcctcgacg
     1381 gcgccaagct gcccgagttc gtggacgcga gcaccttcgt ccccaaagcc atgctgaaca
     1441 tcgccgccat ccagaagcta tatggcgcct gaaatgctcc gggcttcccc gcagtggaac
     1501 atcacggctt gcgtccgcct ccgctatctt ccatgctctg tcctcgatta ctgacatgac
     1561 attaaagctt tcaaccctga gcattctgct tggcttgggc atgggtctgc cgcagatcta
     1621 tggcatcgcc aaaccggccg cattcgccgc ggcggtgcgg aaattcccgc gctccctgcc
     1681 gtggggtata gcgctgatgg tccttggcac ggtctggttc ctgtggaatc tgagccagga
     1741 gtccattgcg gatttcgctt cctacaagaa tgtcttgctc gccgggttcg ccggcgtggg
     1801 tttgggggcc tgcatcttcg tgcaggattt cctggcggtg cgggggctgg cggtcgtgct
     1861 gctgctgctg gccaagttga tggtggatac cggtcgtccc tcgttgccgc agacgcactg
     1921 ggtgatcgtc attcagacgt ggggctacgt attggtggta gccggcatgt ggctaaccgt
     1981 ttcgccgtgg cgcttgcgcg actggctgac atggggcacc gcaaacaaca accgcgtcaa
     2041 ggttgtttgc ggacttaaac tggcgttcgg tttattcgtg gcgtggctgg ggctgaccgt
     2101 attcaagccg ggggtctagg tgcgcgcccg cagtcttgga cgtgttgatc ccgtgaattc
     2161 tttacggggc agaattctgg tcattcgcgg cggggcgatc ggcgacttta tattaacgtt
     2221 gccggcaatc gccgcattgc gccggcaatt tcctcaagcc cacctcgaag tcctcggcta
     2281 cccccatatt gcgcaactgg ctctggttgg cggcctggtg aaccgggtcc agtccatcga
     2341 agcgggcgcg ctggccggct ttttcgcgcg cggcggcgaa ctggctgaga acctggcaga
     2401 ctacttctcc gagttcgatt tgatcctgtc ttatctatac gatccagacg cgatcttcca
     2461 aaccaacgtg gggcgttgca cgggcgccca gttcattgtg gggccgcatc gggtggacga
     2521 agcagcccgg ctccacgccg cgaaagtctt tcttcagcca ctggagcggc tcgccatttt
     2581 cgacgccgac cccatcccgc gcctaccccc tccctctctc aactctcaac tctcaactct
     2641 caacctcctc gccctccatc ccggcagcgg cagcgagcgc aagaactggc cggaagcgaa
     2701 atgggccgag ttgctgcagt atctggtaag ctcaacggag ttcgatttgc tgctcgtggg
     2761 cggggaagcg gaaggcgatc ggctgcaacg cctggccgca gcgctgcccc cggcgcgctc
     2821 ccaagtggcg caaagcgtgc cgctggcaga gttagcgggt cggctggcag cctgccgcgc
     2881 attcgtcggc cacgactcgg ggatttcgca cctcgcggcg gcggtgggct tgccggggtt
     2941 gttattgtgg ggcgacaccg cggaagagat ctggcggcca ccgagtgaaa agatgagggt
     3001 gcttcgccat cctgtgggtc tggaccggtt gccggtagcg gaagtcatgg agcagttgcg
     3061 gagggtgctg gtcgcgagct gcggattgac accgggtttg agataagcag ccaacaacca
     3121 ttgggcaaag gaattgcttc tgggtggctg caaccgggca aaaatgcccc cgcacccgtt
     3181 gcctatagac aacggggcag ccggtgtcct ggctttg
//
LOCUS       DATCGS010000425         3912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_231157, whole genome shotgun sequence.
ACCESSION   DATCGS010000425 DATCGS010000000
VERSION     DATCGS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231157"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VIL39_08770"
     CDS             complement(<1..723)
                     /locus_tag="VIL39_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEY5481556.1"
                     /translation="MADYRPETCAYCLTDTWCEIRANGKPQCRACKVERFFAEILYPP
                     LGYRLLPWQRKVLRDIYGTVSPEDGRRRYRSAYISVGKKNGKSFLIGGLPLYHLLMED
                     EQNPEAYGAAAAKDQAGLVFKAAAQLVAANPHLQARLKVLPSTKRILRYDGGGFYAVL
                     SADGDLQDGIEPSLAIRDEVHRWKTLRAETLRDVLVKGQISRVEPLDIGITTAGAEYE
                     SQLWWREYQQAKKVLDGSLHSET"
     gene            complement(716..1198)
                     /locus_tag="VIL39_08775"
     CDS             complement(716..1198)
                     /locus_tag="VIL39_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEY5481557.1"
                     /translation="MRILEGNRGRRPLPANEPQYPPGVPERPSGMSAGARRIWDTLVG
                     EMAASGVLRTVDALALMQLCEDQAMLDTLRKGMAEMTQEISKKAKEKKMELPGGPLIQ
                     LSRTIEGRRTLSTIRELSAQIIVQRREFGLTPASNGRVQMVGGPGSGFMDPLEQALCG
                     "
     gene            complement(1225..1419)
                     /locus_tag="VIL39_08780"
     CDS             complement(1225..1419)
                     /locus_tag="VIL39_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481558.1"
                     /translation="MTDIERCEQEIAQAEAMLRAGHREVAGLLLQLMDWSIERRLLSG
                     QGTLLEPDGKRSERTEATWA"
     gene            complement(1416..2225)
                     /locus_tag="VIL39_08785"
     CDS             complement(1416..2225)
                     /locus_tag="VIL39_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY5481559.1"
                     /translation="MERACTCKKCGKEFTAPVQGQHKVLCGDSTSAEAVARLMGGQNA
                     DLAFLDPPYNCDYSGYTEEHLRIQNDRMSDGDFKRFLEASFRSLRAAVKPGASVYVCH
                     SSSWQREFQNALEASGFEVRCQIIWAKNTFAWGFGRYKFQHEPIFYCHVAGQKDPWYG
                     DKSQSTLWEEKKPAANRLHPTMKPVELVRRALINSSKSGDVAVDLFGGAGSTLLGCEV
                     TGRICLTMELDPKYADVVVSRWQEFTHRTAVLEGDGRTFAELQAERVVVAA"
     gene            complement(2225..3232)
                     /locus_tag="VIL39_08790"
     CDS             complement(2225..3232)
                     /locus_tag="VIL39_08790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY5481560.1"
                     /translation="MPECRCRKCGKPFDALLKAKHRLLCGDARNREDVERLLAGAKAN
                     VVITSPPYASQRKYDESSDFRPIPPDQYVEWFRDVAMNIAGVLAPGGSFLLNIKEHAD
                     DGQRSLYVKDLTLAHVRQWGWRFVDELCWRKTDEGVPGGWPNRFKNAWEPVFHFTRDE
                     TIKFNPAAVSHLSDDCFEYSPNNPKSTSGSGLLGSGKRGGMAAEQSATGAAMQKTRQS
                     DEQGRFAGLARPSNVIEAKSESGQGSHSAPFPRALVEFFVKAFSDVGDIIFDCFMGSG
                     TSMAAAHVLGRSGYGCEISPAYCDVIVRRMINLGAGSVVLESTGQSFDEVAIERTGVP
                     A"
     gene            complement(3225..3818)
                     /locus_tag="VIL39_08795"
     CDS             complement(3225..3818)
                     /locus_tag="VIL39_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY5481561.1"
                     /translation="MAKRKASSVPVTAPAIWQNRITRYGEAAPDQLLAHERNWRTHPK
                     AQQDALEGALRTVGIVQNVVVNERSGKMLDGHLRVEMAISSGQPTVPITYVDLSDEEE
                     ALILATIDPVTGLAGTDQRLLDSLITDIRLSDLGMELGMGLNDLLNSLSPDPALAAAE
                     GEDEVPAVPENPVSRLGDLWLLGGHVVCPHCGTVNDA"
BASE COUNT          706 a         1287 c         1110 g          809 t
ORIGIN      
        1 cgtctcggag tgtagcgagc catcgagcac ctttttggcc tgctgatact cgcgccacca
       61 gagctgggac tcgtattccg cgccggccgt ggtgatgccg atatccagcg gctccacgcg
      121 cgagatttgc cccttgacga gaacgtcgcg cagggtttcc gcgcgtaacg tcttccagcg
      181 gtgcacctcg tcccggatcg ccaggctcgg ctcgataccg tcttgcaggt cgccgtccgc
      241 tgagaggacg gcatagaaac cgccgccgtc gtaccgcagg attcgctttg tcgagggcag
      301 cactttaagt cgcgcctgga gatgcggatt ggccgccacc agttgcgccg ctgccttgaa
      361 taccagcccg gcctggtcct tggccgccgc tgcgccgtaa gcttctggat tttgctcgtc
      421 ttccatgagc aggtgataga gaggcagccc gccgatcaaa aacgatttgc cgttcttctt
      481 cccgaccgag atgtaggcgg aacgataccg acgccgtcca tcctccggtg acaccgttcc
      541 gtagatgtcg cgcaggacct ttcgctgcca tgggagcagc cggtagccca acggaggata
      601 cagaatctct gcgaagaacc gctctacctt gcacgcccgg cactgcggct tgccgttggc
      661 gcggatctcg caccaggtgt cagtcaggca gtacgcacag gtttcgggcc ggtaatcagc
      721 cacacagggc ctgctccaga gggtccataa agcctgaacc aggccccccc accatctgta
      781 cccggccatt cgaagcgggc gtgaggccga attcccggcg ctgcacaatg atctgcgccg
      841 acagctcccg gatagtgctc aacgtcctac ggccttcgat ggtgcggctc agttgaatga
      901 gtgggccgcc tggaagttcc atcttctttt ccttcgcctt cttcgaaatc tcctgcgtca
      961 tctcggccat gcccttgcgc agcgtgtcca acatcgcctg gtcctcgcag agctgcatga
     1021 gagccaacgc atccacggtg cgcagtacgc cagatgccgc catctcgcct accagcgtgt
     1081 cccagattct ccgcgcgccc gcgctcatgc cgctcggccg ttccggcaca cccggcgggt
     1141 actgcggctc gttggcgggc agcgggcggc gcccgcgatt cccttcgagg atgcgcatgc
     1201 gcgtcggttt cggagggggt cctcttaagc ccacgtcgcc tccgtccgct cgctgcgctt
     1261 gccgtcgggt tcgaggagag tgccttggcc ggataacagc cgtcgctcga tcgaccagtc
     1321 cataagctgc aagagaagcc ctgccacctc acggtggccg gcgcgcagca tcgcctcagc
     1381 ttgggcgatc tcttgttcgc accgctcgat gtccgtcatg ccgccaccac cacgcgctcg
     1441 gcctggagct ctgcgaaggt gcgcccgtcg ccttccagca ctgcggttct gtgtgtgaac
     1501 tcctgccagc gggagactac tacgtccgca tatttcggat cgagttccat cgttaggcag
     1561 attctgcccg tgacctcgca ccccaaaagc gtgcttcctg cgccgccgaa caaatccacg
     1621 gcgacgtcgc cggacttgct gctattgatg agagcgcgcc gaaccaactc caccggcttc
     1681 atagttgggt ggagccggtt ggccgcaggc ttcttctctt cccacagcgt ggattgcgat
     1741 ttgtctccgt accaagggtc cttctgccca gcgacatggc agtagaagat gggctcgtgc
     1801 tggaatttgt aacggccgaa gccccacgca aatgtgttct ttgcccagat aatctggcaa
     1861 cgcacttcga agccgctcgc ctccaacgcg ttctgaaact cgcgctgcca agacgaggaa
     1921 tgacaaacat agaccgatgc accgggcttt acggctgcgc gaagagatcg aaatgaagct
     1981 tccaggaacc gcttgaaatc accgtccgac atccgatcat tctgaatcct caagtgctct
     2041 tcggtatagc ctgagtaatc gcagttgtag ggagggtcaa gaaatgccaa atcagcattc
     2101 tgcccgccca tcagccgcgc cacggcctcc gcgctggtgg agtcgccgca taggacttta
     2161 tgctggcctt ggaccggcgc ggtgaactcc ttgccgcact tcttgcaagt gcatgcgcgt
     2221 tccatcacgc gggcactcct gtgcgctcga tggcgacttc atcaaacgac tggccggtgg
     2281 attccagcac taccgatccg gcgcccaggt tgatcatcct gcgcacaatg acatcgcagt
     2341 aggcgggcga gatctcgcag ccgtaaccac tgcgacccaa gacgtgcgcc gcagccatgc
     2401 tggtccccga tcccatgaag cagtcgaaaa tgatgtcgcc gacgtcggag aaggctttca
     2461 cgaagaattc cacgagggcg cgagggaacg gtgcggagtg ggagccctgc ccgctttctg
     2521 atttggcttc gatcacatta gaggggcggg ctaacccagc gaatcgccct tgctcgtcag
     2581 actgccgggt cttttgcatg gctgcacccg tggcggattg ttcggccgcc attccgccgc
     2641 gcttgccaga tccaagcaat cccgagccgc tggttgattt gggattgttc ggcgagtact
     2701 cgaaacaatc gtccgaaagg tggctgacgg cagcgggatt aaacttaatc gtttcgtccc
     2761 tggtgaagtg aaagaccggc tcccacgcgt tcttgaaccg gttcggccag ccgcccggca
     2821 cgccttcgtc tgttttccgc cagcagagtt cgtccacaaa ccgccagccc cactgccgga
     2881 cgtgggccag tgtcaaatcc tttacgtaca gactccgctg tccatcgtct gcatgctctt
     2941 tgatattcaa gagaaacgat ccacctggag ccaggactcc tgctatgttc atcgccacgt
     3001 cccggaacca ctccacgtac tgatcaggcg gaatcggtcg gaagtcgctc gattcgtcat
     3061 acttcctctg gctggcatag ggaggcgatg tgatcacaac gttggccttg gcgccagcga
     3121 gcaaccgttc cacgtcttcg cggttgcgcg catccccgca gagcaaacgg tgcttcgcct
     3181 tcaacagcgc atcaaacggc ttcccgcatt tcctgcaacg gcactcaggc atcgttcacc
     3241 gtcccgcaat gcggacatac gacgtggccg cccagaagcc acaggtcgcc cagccgcgat
     3301 accggattct ccgggaccgc cggcacctcg tcctcgccct cggcggccgc cagtgccggg
     3361 tcgggtgaaa ggctattcag caggtcattc aatcccatcc ccaattccat gccgagatcg
     3421 ctcagccgga tgtccgtgat gagggaatcc agcaacctct ggtcggtgcc ggcaagccct
     3481 gtcaccgggt caattgtggc gaggatcagc gcttcttctt cgtccgaaag gtccacgtat
     3541 gtgatcggaa cggtgggctg tccagagctg attgccatct cgacgcgaag gtgaccgtcg
     3601 agcatcttcc cgctacgctc gttcaccacg acattctgga cgatgcctac tgtgcggagc
     3661 gcgccctcca gcgcgtcctg ctgagccttc ggatgggtcc gccaatttcg ctcgtgggcc
     3721 agcaactggt cgggcgctgc ctcaccgtag cgagtgattc ggttctgcca aatcgccggc
     3781 gccgtcaccg gtacgctgct cgctttccgc ttcgccatca gacgctcctc gcttttgctt
     3841 gcactccgcc agaggttttc atgctgcacg ttccgacttt tccaaaatgc gacgaatctt
     3901 cagtgccagc cg
//
LOCUS       DATCGS010000426         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_231241, whole genome shotgun sequence.
ACCESSION   DATCGS010000426 DATCGS010000000
VERSION     DATCGS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231241"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIL39_08800"
     CDS             <1..193
                     /locus_tag="VIL39_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481562.1"
                     /translation="REDESRVRQPNAASVLGIFRRLSNAFKQLWAEGRPKREATSRDW
                     VEENHFNRWRGIRLITQTT"
     gene            331..453
                     /gene="cas2"
                     /locus_tag="VIL39_08805"
     CDS             331..453
                     /gene="cas2"
                     /locus_tag="VIL39_08805"
                     /inference="COORDINATES: protein motif:HMM:NF021356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY5481563.1"
                     /translation="MARTADYVAVYDVSNDAERTRVAKVLEGFGIRVQMSAFEC"
     gene            478..618
                     /locus_tag="VIL39_08810"
     CDS             478..618
                     /locus_tag="VIL39_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481564.1"
                     /translation="MKKRLADLKLETGFVYLYRRDALSRRVGIGQCPENPFDDGRYAY
                     VI"
     gene            602..1516
                     /gene="cas6"
                     /locus_tag="VIL39_08815"
     CDS             602..1516
                     /gene="cas6"
                     /locus_tag="VIL39_08815"
                     /inference="COORDINATES: protein motif:HMM:NF021551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HEY5481565.1"
                     /translation="MLTSSESTPRATAESAGLAELLSLRRWRTRVVLRNTRNEFAREH
                     PLWVFHGLLMHALPKDAEGKPKLRFFFHPATEALGDRIQKGREYTLDVILPTADPSPA
                     AEFSERLAAHLAVRHSSGPGRNFELVRAGPPELRSLAAVAAENPPPPADTEEVCLEFI
                     TPLAYQPRDERRKWLFTSAELFPAMIRRWEKLFGITLPRFEEDWSATELLPYFWHYER
                     HRHQPKSSAGCVFLNGNVGPLYLRGPVTRLWPLLLLGSELHVGLEMPAAQGYFQLLLR
                     RPFFDASLLNPSSYEAALNHLTERSDLG"
     gene            1503..3443
                     /gene="cas1"
                     /locus_tag="VIL39_08820"
     CDS             1503..3443
                     /gene="cas1"
                     /locus_tag="VIL39_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HEY5481566.1"
                     /translation="MTWDELAQSLDDRATTLAKLAEAVRDGAWQPSPARSFLAKKSTG
                     DGTRTISLLTARDHLVHKVLLETLAPILDRAFEQASLGYRPGKSSEDVRAAIERAAAE
                     GCGYVLESDIEGFFDHVNWDLLLDRLDRHVPRADVVTRRLLRQCIAHPVEFNGAPATR
                     TGGLLQGSPLSPLLSNLYLDHFDEQMEARGFRLIRYADDFLVLARTAEECEQALAAVR
                     EILASLKLSVKPEKTSVTVFDAGFSFVGLQFGQGLDAGMLSRAALKKTVFLRHEFCFV
                     GADHDALVVRKGDGLVARLPLRRVGELILLGASGVSTRLVEKCDWLDIPITFCTPAGK
                     FISTIAGSDRGHYEMAFRHAQRRQGLAEPEVVAITRRLVTAKLSHYGAWLRREGGAEA
                     GLALAAINESLEGLAEATNVDTVRGWEGHAAAATFVCLNGRVRDSAFSSSKREPHQQR
                     DRWNSLLDLAYSMLFARLNTLLRTRGLCPFLGFLHSPINRFESLVCDLQEPFRARMDR
                     FVLKLVNLGSIQEKDFQKLDDGRFFLESAAMGRFLEAFEQEMKTRLRSEPGNLEQLLL
                     GQVYAVEGWVKGTEELRFYQADYQPGRRCRPGTPTPNKSKHTQLGPVPAATAGSSSGS
                     PPPAVQGAELPRGEQVGHQPIP"
     repeat_region   3670..4044
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3670..3705
                     /rpt_unit_seq="gtcaggaaggctgtggggtgcgcgcctcgttgtgac"
BASE COUNT          763 a         1210 c         1285 g          822 t
ORIGIN      
        1 ccgcgaagac gagagtcggg tgcgtcagcc caacgctgcc tcggtcctgg ggatctttcg
       61 gcgcctctcc aacgccttca aacaactgtg ggccgaaggg cggcccaagc gggaggcgac
      121 ctcgcgagat tgggtggagg agaatcactt caaccgttgg agaggtatcc gtcttatcac
      181 tcagaccact tgatctaatc ctaaagtgcc caggaaccgt aaatccccgc gctggcatag
      241 cctgaaccga gcttgatgca gccctggcgc ctcgttgtgg cctgagggaa tggcttgact
      301 ttccgtcagg ggttccggca aataccctgc atggctcgca cggctgatta tgtcgcggta
      361 tatgacgtct cgaatgacgc cgagcgcacg cgcgtggcga aggtgctgga ggggttcggc
      421 attcgggtgc agatgagcgc gttcgagtgc tagttgacgc gcagcgggcg ggaccagttg
      481 aaaaaacgcc tggccgacct caagctggaa accggtttcg tttacctcta ccgccgcgat
      541 gccctgagcc ggagggtcgg tatcggccaa tgtccggaga acccatttga tgatggccgt
      601 tatgcttacg tcatctgaga gcacgccacg cgcaacagcg gaaagcgccg gtctggcgga
      661 gttgttgtcg ctgcggcggt ggcggacgcg cgtcgtgttg cgcaacacgc gcaatgagtt
      721 tgcccgggaa cacccactct gggtttttca tggcctgctc atgcacgccc tgccgaagga
      781 cgcggaagga aagcctaaac tacgcttttt ctttcatccc gcgacggaag ccctgggcga
      841 ccgcatccaa aaagggcgcg agtacacttt ggatgttatc ctcccgacgg ctgacccgtc
      901 gccggcagcg gagttcagcg aacgactggc ggcacatctc gcggtgcgac actcctcggg
      961 cccgggccgc aacttcgaac tcgtgcgcgc tggaccgcca gaactgcgca gcctggccgc
     1021 agtggccgcc gaaaatcctc ccccgccggc agacactgag gaagtgtgcc tggaattcat
     1081 cacgccgctg gcttaccagc cgcgcgacga acggcgcaag tggctgttca cgtccgccga
     1141 gttgtttcca gccatgatcc ggcgctggga gaaactgttt gggatcacgc tgccgcgttt
     1201 tgaagaggat tggtccgcca cagaactgct gccctatttc tggcattatg agaggcatcg
     1261 gcaccagcca aaaagctcag ccggctgcgt cttcttaaac ggcaatgtgg gtccacttta
     1321 cctgcgcgga cccgtgaccc gcctctggcc cctgctgctg cttgggtcgg aattgcacgt
     1381 ggggttggaa atgcccgcag cccagggcta ttttcaactc ctgctccggc gccctttttt
     1441 cgacgccagc ttgttgaacc cttcgtccta tgaggccgcg ctcaaccacc tgaccgagcg
     1501 cagtgacttg ggatgaactg gcgcaaagcc tcgatgaccg cgcaactacg ctggccaaac
     1561 tggccgaagc ggttcgagat ggcgcatggc aaccctcccc ggcgcggtcg ttcctcgcca
     1621 agaagtccac cggcgacggc acgcgcacga tctccctgct caccgcgcgt gaccacctgg
     1681 tgcataaagt cctgctcgaa acactcgccc ccatcctgga ccgcgccttc gagcaggcct
     1741 cgcttgggta tcgccccggg aaatcttcgg aagacgttcg ggccgccatc gagcgcgccg
     1801 ccgcggaagg ctgtggctac gttctggaat cggatatcga ggggttcttc gaccacgtca
     1861 attgggatct cttgctggat cgcctggacc ggcacgtacc gcgcgcggac gtggtgaccc
     1921 gccgtctgct gcgccagtgc atcgcgcatc ccgtggagtt caacggtgcg cccgcgacgc
     1981 gcaccggcgg gctcttgcaa ggcagcccgc tttcgccgct gctctctaat ctctacctcg
     2041 accacttcga cgagcagatg gaggcgcggg gattccggct catacgctat gcggatgatt
     2101 tcctggtgct ggcccgcacg gcggaggaat gcgagcaggc cctggccgcc gtgcgcgaaa
     2161 tcctcgccag cctgaaactg tcggtgaaac cggaaaagac cagcgtgacc gtttttgatg
     2221 cgggtttcag tttcgtgggt ctgcaattcg gtcagggcct tgacgccggg atgttgagcc
     2281 gggccgcgct gaagaagaca gtgttcctcc ggcacgagtt ttgttttgtc ggggcggatc
     2341 acgatgcgct ggtggtgcgg aagggtgatg ggctggtcgc ccgtctgccg ttgcggcggg
     2401 tgggcgaatt gatcctgctg ggcgccagcg gcgtgtccac ccgcctggtg gagaaatgcg
     2461 attggctcga cattccgatc accttctgca ctccggcggg aaagttcata agcaccatcg
     2521 ccggtagtga tcgggggcat tatgagatgg cgttccgcca cgcccagcgc cggcaaggcc
     2581 ttgccgagcc ggaggtggtg gcgatcaccc ggcggctggt tacggccaaa ctctcgcatt
     2641 atggggcgtg gctccggagg gaaggcggcg cggaggccgg cctcgcactc gcggccatca
     2701 acgaatccct cgagggcctg gccgaagcaa ccaacgtgga tacggtgcgc ggttgggaag
     2761 gtcacgccgc ggcggccact tttgtgtgcc ttaacgggcg tgtgcgcgac agcgcgttca
     2821 gcagttcgaa gcgggagccg caccagcagc gtgaccggtg gaattctctg ctagacctgg
     2881 cgtactcgat gctctttgcg cgcctgaaca cattgctgcg cacgcgcggt ctgtgcccgt
     2941 tcctgggatt tctccacagc ccgatcaatc ggtttgagtc gctcgtttgc gatttgcagg
     3001 aaccgttccg cgcccgcatg gaccgttttg tgctcaagct ggtcaacctg ggcagcattc
     3061 aagagaaaga cttccagaag ttggacgacg ggcgcttctt cctcgaatcg gcggccatgg
     3121 gccggtttct cgaagcattc gaacaggaga tgaagacgcg gctgcgaagc gagccgggca
     3181 atttggagca actactactc ggacaagtct atgctgtcga gggctgggtc aaaggcaccg
     3241 aggagttgcg tttctaccag gccgactacc aacccggacg acgctgccgc ccggggacgc
     3301 cgaccccgaa taagtccaag cacacgcagc tcggtcccgt tccggcagca actgctggtt
     3361 cctcatccgg gtccccgccg ccagccgttc aaggcgccga actacctcgc ggagaacagg
     3421 ttgggcacca gccaattccc tgactttcaa aatgcacttc aacttgcttc cgaatgatcg
     3481 gccagagttg cctggggcaa gatccgtgcg caactccttg aaaatcaagg tttcgagttt
     3541 tgaccgagtg ttaagaattg accatttcag aaacctgccc aattccggtg ctaatccggg
     3601 tcgaaaaaca gaggttaccg gaaaacggcg cttgaacttc tttataatca acggtacaat
     3661 aggaggacag tcaggaaggc tgtggggtgc gcgcctcgtt gtgacgttga cataagccaa
     3721 tatcagaacc tccttaggtc aggaaggctg tggggtgcgc gcctcgttgt gactctggtc
     3781 gaggcggccc ttggccagcg tgtatgtcag gaaggctgtg gggtgcgcgc ctcgttgtga
     3841 cagaggatgt tctagtactc atattcagtt actgtcagga aggctgtggg gtgcgcgcct
     3901 cgttgtgacg ttaaaaagac cacagtgccc aatatcagag tgtcaggaag gctgtggggt
     3961 gcgcgcctcg ttgtgacaac gtagccctat cggttgtcag aaattaaagt caggaaggct
     4021 gtggggtgcg cgcctcgttg tgaccctttg gtgccgtcgt tacattctgc tgggtcagga
//
LOCUS       DATCGS010000427         4217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_532357, whole genome shotgun sequence.
ACCESSION   DATCGS010000427 DATCGS010000000
VERSION     DATCGS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532357"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VIL39_08825"
     CDS             complement(<1..434)
                     /locus_tag="VIL39_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481567.1"
                     /translation="MTALFRVFYRLYRLVSGRWHWAQRQFTRPGLAVLGALGLVALLA
                     PDTDNNLTYQALPLLLFLLVVAVCLSRLSQVRFSATRVLPRFGTVARPLNYPIVVKNL
                     SGKAQSGLTLLENLADSRPSFEDWVALERAEQKLLRSFQFSTP"
     gene            complement(461..1441)
                     /locus_tag="VIL39_08830"
     CDS             complement(461..1441)
                     /locus_tag="VIL39_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5481568.1"
                     /translation="MSQKPEPILTLTQPDREAAREVYRRITANIAKIMQGQAASTRKL
                     MAAFASGGHVLLEDFPGTGKTTLAKALARSVDAKFKRIQFTPDLLPSDILGVSIFSQR
                     DQQFHFHEGPIFSNILLADEINRASPRTQSALLEAMGESQVSVDGERRDLEDLFFVIA
                     TQNPVEFRGTYPLPEAQMDRFGMQFTLGYVQSAEEVAILTAQEHNHPLNELKPCASLA
                     DVLGLKRAVQEVRISDELKRYAVDLAGATRTAPGVQLGASPRASIALMKAAQALALFE
                     GLDFVMPEQIQELAVPVIAHRVVMEPQARFSGQTARSVVEEIVKKTRVPA"
     gene            1538..2722
                     /locus_tag="VIL39_08835"
     CDS             1538..2722
                     /locus_tag="VIL39_08835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5481569.1"
                     /translation="MKVAFYGHVRQYHNIKSEIDKNIKTVLESGQYVMGPMLKQFEAE
                     LAAYHGTKYAVGTGNGTDAIWLALMALGIGPGDEVITHPNTFFATAEAIWIAGATAVF
                     VDCCPTTKCICPKAIEAAITPKTKAIIPVHLYGQCADMVAISKIAKKHKLKVIEDNAQ
                     SIGARGPNFKIGQLSDAATTSFIIQKNLGTFGDSGGLVTNNADIDAAVRRLRNHGSTA
                     RNVHSYGFNSRLDDLHAGILSAKLKHIDEYNDARRNWAARYTKGLKDCKTFSLPVELK
                     GYRHVFHLYVIETMKPEWRDQLVAFLVKNGIDAKTHYSIAIHKQAGYPWGKKARIVGS
                     LANAEANAATCVSLPIFPELTAKEVDYVIAKVKEWDQQFASQVPAGKGSAKSGAACCC
                     SK"
     gene            2829..3902
                     /locus_tag="VIL39_08840"
     CDS             2829..3902
                     /locus_tag="VIL39_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481570.1"
                     /translation="MNKYKVLVVGMGKRGMHHATAFNANPKFQVAGICDIDPKRLDDA
                     AAKLGNPQKGTDAAALAKAVKPDVFCFCTLPNLRTPMIKAAIGGGAKLIAFEKPAALT
                     SAELFTIRDLLKKAGVKAVVSHQHRYGTHYQKVKEIIASGALGRVHTVYASATGWMTH
                     MLSHLVDYTCWFNNYTPASWAMAQAAGRAKLGDNHPSPDYIGGFVQFANGVRGIYECG
                     AGAPDQPEVAKWWGKNRIGAQGTEGFAEVLTNGGWRAVTKSGGYQSGDGAMNYDHDMP
                     PYIQEMADWLDDGKKVHQCNFDHACLGAEIMLAMQRSAAEGGQIALPLASGADEQALL
                     KAKLADRPVLVSCEQNKKEFGLA"
BASE COUNT          861 a         1407 c         1208 g          741 t
ORIGIN      
        1 ggggtgctga attgaaacga acggagcaac ttctgctcgg cccgctccag ggctacccag
       61 tcctcaaacg acggtcgcga gtccgccagg ttctccagca gggtcaggcc gctctgagct
      121 ttcccggaga ggttcttcac cacgataggg tagttgagtg ggcgggcgac cgtcccgaac
      181 ctcggcagca cgcgggtcgc ggagaaccgc acctgggaaa gccgactcaa gcaaacagcc
      241 acgaccaaaa gaaacaacag gagcggcaga gcctggtagg taagattgtt gtcggtgtct
      301 ggcgccagca aggcgacgag tcccagcgcg cccagcacgg ccagtcccgg tcgcgtaaat
      361 tgccgttgcg cccaatgcca ccgcccggaa acaaggcggt aaaggcggta gaaaactcgg
      421 aacagggcag tcatggatag tccgggcttc ggacttccgg ttacgccggc acccgggtct
      481 tcttcacgat ttcctccacg acgctgcgcg cggtctggcc ggaaaaccgc gcctgcggct
      541 ccatcaccac ccggtgcgcg atgactggca ccgccagctc ctggatttgc tcgggcatga
      601 cgaaatccag gccctcgaac agcgccaggg cctgcgccgc tttcatcagc gcgatggacg
      661 cgcgcgggct ggcgcccagt tgcacgcccg gcgccgtccg cgtcgccccc gccaaatcca
      721 ccgcatagcg ctttagctcg tcgctgatgc gcacctcctg cacggcgcgt ttcaagccga
      781 gcacgtccgc cagcgaggcg cacggcttca gttcgttgag cggatggttg tgttcctgcg
      841 ccgtcaggat ggcgacctct tcggccgact gcacatagcc cagcgtaaat tgcatcccga
      901 accggtccat ctgtgcctcc ggcaacggat aggtgccgcg aaactccacc gggttctgtg
      961 tggcgatgac gaagaagagg tcctccaagt cccggcgctc gccgtccaca ctcacctggc
     1021 tctcacccat cgcctccaac aaggccgact gggtgcgggg cgaggcgcgg ttaatctcat
     1081 ccgccagcag gatgttggag aagatcggcc cctcatgaaa atggaactgc tgatctcgct
     1141 ggctgaagat tgacacgccg agaatatccg agggcaacag gtcgggtgtg aactggatgc
     1201 gcttgaattt tgcgtccacc gaccgcgcca gggccttagc cagcgtcgtc ttgccggtac
     1261 cgggaaagtc ctccagcagg acatggccgc cgctcgcgaa cgcagccatc agcttgcgcg
     1321 tcgaggccgc ctgcccttgc atgattttgg cgatgttggc cgtgatccgc cggtaaacct
     1381 cgcgcgccgc ctcgcggtca ggctgcgtca gagtgagaat tggctctggc ttctgagaca
     1441 tgtcgctatg gtaatcgctg cctgtatttg cggcgaattt atttaatccg atagaaagcg
     1501 cttgtttttg cgccgcggtc tagtagcttc gcttccaatg aaagttgcat tttacggtca
     1561 cgtccgacag taccataata tcaagtcgga aatagataag aacattaaga cggtgctgga
     1621 gagcggccaa tacgtcatgg gccccatgct caagcagttt gaagccgaac tcgccgccta
     1681 tcacggcacc aaatacgcgg tcggtaccgg caacgggact gacgccatct ggttggccct
     1741 gatggccctt ggcatcggac ccggcgacga ggtcatcacc cacccgaaca ctttcttcgc
     1801 caccgccgaa gcgatctgga tcgccggcgc cacggccgtc tttgtggatt gctgccccac
     1861 gaccaagtgc atctgcccga aagcgattga agccgccatc acccccaaga ccaaggccat
     1921 catccctgtc cacctttacg gccagtgcgc cgacatggtc gctatcagta agattgccaa
     1981 gaagcacaag ctcaaggtca tcgaggacaa cgcccagtca atcggcgcgc gcggaccgaa
     2041 cttcaagatc ggccagctca gcgatgcggc gaccaccagc ttcatcatcc agaagaacct
     2101 tggcaccttc ggcgacagcg gcgggctcgt caccaacaac gccgacatcg atgccgccgt
     2161 ccgccggttg cgcaaccacg gctcgaccgc gcgcaatgtc cacagttacg gcttcaacag
     2221 ccgcctggac gacctccacg ccggcatcct gagcgccaag ctcaagcaca tcgacgagta
     2281 taacgacgct cgccgcaatt gggcggctcg ctacaccaag ggcctcaagg attgcaagac
     2341 cttcagcctg ccggtcgagc tgaagggcta ccgccatgtg ttccacctct acgtcatcga
     2401 gaccatgaaa cccgagtggc gcgaccagtt ggtcgctttc ctggtcaaga acggcattga
     2461 cgccaagacc cattactcca tcgctatcca caagcaagcc ggctacccct ggggcaagaa
     2521 ggctcgcatc gtcggcagcc tggccaacgc cgaggccaac gccgccacct gcgtcagctt
     2581 gccgatcttc cctgagctta ccgcaaagga agtggactac gtcattgcca aggtcaagga
     2641 atgggaccag cagtttgcct cccaggtccc ggccggcaaa gggtccgcca aatccggcgc
     2701 cgcctgctgc tgctccaagt agcattgaat taagcttacg cacgggcgcc acgggatcca
     2761 cccctggcgc ccgttttcac accatcccca tcacgtatca cgtatcacgc atcacgcatc
     2821 atccccttat gaacaagtac aaagtcctcg tcgtcggcat gggcaagcgc ggcatgcacc
     2881 acgccaccgc cttcaacgcc aaccccaaat tccaggtcgc cggcatctgc gacattgacc
     2941 cgaagcgcct ggacgacgcc gcggccaagc tcggcaaccc gcaaaagggc accgacgccg
     3001 ccgccctggc gaaggcggtg aagccggacg ttttctgctt ctgcacgctg cccaacctgc
     3061 gcacgccgat gatcaaagca gcgatcgggg gcggcgccaa gctgatcgcc ttcgagaagc
     3121 cggctgcttt gaccagcgcg gagctgttca ccatccgcga cctgttgaag aaagcgggcg
     3181 tgaaagccgt cgtcagccat cagcaccggt acggcacgca ctaccaaaag gtgaaagaga
     3241 tcatcgccag cggcgcgctg ggccgcgtcc ataccgtcta tgccagcgcc accgggtgga
     3301 tgacgcacat gctttcccat ctcgttgact acacctgctg gttcaacaac tacaccccgg
     3361 cgagctgggc gatggcccag gccgcgggcc gcgctaaact cggcgacaat catccgtcgc
     3421 ccgactacat cggcggcttt gtgcaattcg ccaacggcgt ccgcggcatt tacgaatgcg
     3481 gcgcgggcgc gcccgaccag ccggaggtcg cgaagtggtg gggcaagaac cgcatcggcg
     3541 cgcagggcac cgaaggcttt gccgaagtcc tcaccaacgg cggctggcgc gcagtcacga
     3601 agagcggcgg ttaccagagc ggcgatggcg cgatgaacta cgaccacgac atgccgccct
     3661 acatccagga gatggccgac tggctggacg acggcaagaa agtgcaccaa tgcaattttg
     3721 accacgcctg cctgggcgcc gaaatcatgc tcgccatgca gcgcagcgcc gccgagggcg
     3781 ggcagatcgc cctgccgctc gccagcggcg ccgacgagca agccctgctc aaagccaaac
     3841 tggccgaccg ccccgtgctc gtctcctgcg agcagaataa gaaagaattt ggtctggcct
     3901 gagagcgtcg agcgcagccc ggagttcctg cctttctgcg ctctgccctc taccatctac
     3961 cttcgaccgt cgcttccaag tggcttgagg ctggcctgcc cggccactga tcaccgatca
     4021 ctgattactg actaccccct tcttcatcct gccctctgcc ttcgctcagc ggcggccttc
     4081 cccggccatc tgccatctgc catcacccgt gggcagagtt tacccggttc catgcgtgta
     4141 atttgggcga tttgttggag gagtgaatgc ggtgtcggat gagtgctcat gcccatagta
     4201 tatacccatg tatatat
//
LOCUS       DATCGS010000428         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_283777, whole genome shotgun sequence.
ACCESSION   DATCGS010000428 DATCGS010000000
VERSION     DATCGS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283777"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1311)
                     /locus_tag="VIL39_08845"
     CDS             complement(28..1311)
                     /locus_tag="VIL39_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481571.1"
                     /translation="MLATQELIQEAALPYRLGEVLPRWLREVPLYQRSGELSPLGGGA
                     ISLAELRRLPLITKPDIRRDFPRNFLRAGVELDTLLDQDLVELEQTSGTSEERTPLLL
                     GRGWWAEQEERALRLNPYVASVLDEFPEARRVTVNSPVCSGDICYSGVPSRADRIVGN
                     ALFVSLSRYPFLLGEAELARMAAEAVEWQPQFLDVDPVYGVLFALYCERRGIRLPSLR
                     FILGSYEFVSVVHRRILERVFGVPVFNLYGSTETGHLLMEVGRGEMRASLDTALLEAL
                     NTDSQGVSELVVTTLTNDFMPLIRYRIGDLAERHERPYYTSYVLHGRAKDAFAASDGR
                     RVTTWQLDQCLANLAGIAHYQLCERTGGEWLLRFVPDITAPRAEDMAELRQRLARLLG
                     VSGKLAVQQTDLLVPESSGKFRLGYPAKKDALRIY"
     gene            complement(1480..2301)
                     /gene="amrB"
                     /locus_tag="VIL39_08850"
     CDS             complement(1480..2301)
                     /gene="amrB"
                     /locus_tag="VIL39_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5481572.1"
                     /translation="MATPETIDPGKVRPPAVAGRFYPADPVALRQLITDLLAQVPPTT
                     GPAPKALIAPHAGYLYSGPIAASAYAQLISARDQIKRIVLFGPSHYVALHGLATTSAE
                     AFATPLGIVPVDAEAVRQVRALPQVKELDEAHAQEHSLEVQLPFLQCVLGDFKLVPLA
                     VGDATPEEISQALDALWGGPETRFVISSDLSHYHDFKTAQRLDRATAKAIEALKPGGI
                     GEESACGRMPIRGFLQSAHRHGLRARTVDLRNSGDTAGPRDKVVGYGAFVFEEGR"
     gene            complement(2302..>3116)
                     /locus_tag="VIL39_08855"
     CDS             complement(2302..>3116)
                     /locus_tag="VIL39_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5481573.1"
                     /translation="ALRDDAALAPIQTITPQQLVSTGRRLRARLVVSSYNEPLITAEW
                     AVAVFREAKEAGLDCAFVSNGNATPEALDFLRPWIVAYKIDLKGFDDKRYRTLGGTLA
                     QVTENIRMIHERGLWLEVVTLVVPGFNDSESELRQIAQFLASVSRDIPWHVTAFHKDH
                     KMTDAPNTTAAQLIRAAEIGVEAGLRFVYAGNAPGHVGPWENTYCPNCREKLIDRFGY
                     LVREYKLTPDGKCPSCRTPIPGIWPAIGAVQVPTGESMADYYSRLPRGVKVN"
BASE COUNT          563 a          978 c         1033 g          542 t
ORIGIN      
        1 ttgattttgc gtccggcgct cacgtcttca atatattctt agagcgtcct tcttcgccgg
       61 atagcccagc cgaaacttcc cagaactctc cggcaccagc aaatccgttt gctgaaccgc
      121 cagcttgccg ctaaccccga gcagacgtgc caggcgttga cgcagctccg ccatatcctc
      181 ggctctgggc gcggtgatgt cgggcacaaa acgcagcagc cattcgccac ccgtgcgctc
      241 acacaattgg tagtgggcga tgccggccaa gttggccaaa cattggtcca actgccaagt
      301 cgtgacgcgc ctgccgtccg acgcggcaaa ggcatccttc gctcggccgt gcaggacgta
      361 gctcgtgtaa tagggccgct catgccgctc cgccaggtcg ccgatgcggt agcggatgag
      421 cggcatgaaa tcattcgtaa gagtcgtgac gaccagctcg ctgacgccct ggctgtcagt
      481 gttcagcgct tccagcagcg ccgtgtcgag gctcgcccgc atttcgccgc gcccgacctc
      541 catgagcaga tggccggtct cggtcgagcc gtagaggtta aacaccggca ccccgaacac
      601 gcgctcgaga atccgccggt gcacgacgct cacaaactca tagctgccca ggatgaaccg
      661 cagcgacggc aggcggatgc cccgccgctc gcaataaagc gcgaacagca cgccatagac
      721 cgggtccacg tccaggaact gcggctgcca ttcgaccgcc tccgccgcca tgcgcgccag
      781 ctcggcttcg cccaggagaa acggataccg cgagaggctg acgaagagcg cattgccaac
      841 gatgcggtcc gcgcgcgacg gcacgcccga ataacagatg tccccgctgc agaccggaga
      901 gttgacggta acgcggcgcg cttcgggaaa ctcgtcgagc acactggcta cgtatggatt
      961 gagccgcagc gcccgctcct cttgctccgc ccaccagccg cgccccagca gcaaaggggt
     1021 gcgctcttcc gatgtgccgg aggtttgctc cagttccacc aaatcctgat cgaggagtgt
     1081 atcgagctcg acgcctgcgc gcaggaaatt gcgcgggaaa tcacggcgga tgtctggttt
     1141 ggtaatcagc ggcaggcggc gcaactcagc caatgaaata gccccgccgc ccaacgggga
     1201 cagctcgccg gaccgctggt agagcggcac ttcgcgcaac cagcgcggca gcacttcgcc
     1261 cagccgatag ggcagggcgg cttcctgaat cagttcctgc gtcgccaaca tgcaacaatg
     1321 ctttctttca taatccgaat gacgcgcaaa tctagcacaa aatcaagccg cgtgcagact
     1381 cgcaggctgg attcgggggt tcatgaaatc gtagccgctg agggcagggc gtgttgccca
     1441 aacccttttg gcgtagtgcg ccgcttcgag cggcccacct taccggcctt cctcgaagac
     1501 aaacgctccg taaccgacga ctttgtcgcg ggggccggcg gtatcgccgg aattgcgcaa
     1561 atccacggtg cgggcgcgca ggccatgccg gtgtgctgac tgcaggaagc cgcgaatggg
     1621 catccggccg caggcgctct cttcgccaat gccgcctggc ttcagcgcct caatggcttt
     1681 ggcggtggcg cggtcgaggc gctgggcggt cttgaaatcg tggtagtggc tgaggtcgga
     1741 gctgatgacg aagcgggttt cagggccgcc ccaaagggcg tcgagggcct ggctgatttc
     1801 ctcgggggtg gcgtcgccga cggccagcgg caccagcttg aagtcgccca ggacacattg
     1861 gagaaagggc aactgcactt cgagcgaatg ttcctgcgca tgggcctcgt ccaactcctt
     1921 gacttgcggg agcgcccgaa cctgtcggac ggcttcggca tccaccggca cgatcccaag
     1981 cggcgtggcg aaggcttcgg cggaagtcgt ggccagaccg tgcagggcca cgtaatgcga
     2041 cggcccgaac aggacgatgc gcttgatctg atcgcgcgcg gagatgagtt gggcgtaggc
     2101 ggaggcggcg atgggcccgg agtaaaggta accggcgtgc ggcgcgatga gcgctttggg
     2161 cgccggtccg gttgtcgggg gcacctgggc cagcaggtcg gtgatgagct ggcgcagggc
     2221 gaccggatcg gcgggataga agcggcccgc gacggctggt ggccggactt tgccggggtc
     2281 gattgtttca ggggtcgcca cttagttcac cttcacgccg cgcggcagtc ggctgtaata
     2341 gtcggccatg ctttcgccgg tcggcacctg gaccgcgccg atggccggcc aaatgccagg
     2401 aatgggggtg cggcagctcg ggcatttgcc atccggcgtc agcttgtatt cgcgcaccag
     2461 gtagccgaag cggtcaatca gcttctcacg gcagttgggg caataggtgt tctcccaggg
     2521 gccgacgtgg ccgggggcgt tgccggcata gacaaagcgc aggcccgctt cgacgccgat
     2581 ctcggccgcg cggatgagtt gcgccgcggt cgtgttcggc gcgtcggtca tcttatggtc
     2641 tttgtgaaag gccgtgacgt gccaggggat gtcgcggctg acggaagcga ggaactgcgc
     2701 aatctggcgc agctcggatt cgctgtcgtt gaagcccggc accaccaagg tgaccacctc
     2761 gagccagagc ccacgctcgt ggatcatgcg gatgttttcg gtgacctgcg ccagagtgcc
     2821 gccgagcgtc cggtagcgtt tgtcgtcgaa gcccttcagg tcaatcttgt aagcgacaat
     2881 ccagggacgg aggaaatcca gcgcctcggg cgtggcgttg ccattcgaga cgaaagcgca
     2941 gtccagaccc gcttccttgg cttcccggaa gacggccacg gcccattcgg cggtgatgag
     3001 cggctcgttg tagctcgaga cgaccaggcg cgcgcgcaag cgccggccgg tgctcaccag
     3061 ttgctgcggg gtgatggttt ggatgggcgc cagcgccgcg tcgtcccgga gcgcct
//
LOCUS       DATCGS010000429         9862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_630331, whole genome shotgun sequence.
ACCESSION   DATCGS010000429 DATCGS010000000
VERSION     DATCGS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630331"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1791
                     /locus_tag="VIL39_08860"
     CDS             181..1791
                     /locus_tag="VIL39_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5481574.1"
                     /translation="MNRTAKQFEGLLAHYFEALLHDNPTFSTIGAGLRDGEGKLGRLT
                     LDFQKRRERERQSALRALEGISPRELTSEQQLDRLALRSLLLKECEDYARGRHALEPN
                     APDQLLNILLHELMRGDDEPRRGARNLRSLLRQAPDFLDEAAAVVRSPERVWLRVMEQ
                     TVAGGMELLEGVGKFLQTVAPQAGDAVLVSAAGKAMQRYRARVKQRSLAPAGSFAIGA
                     AALQRRVRDELGLDYTLGQVEALALGEVERVGTLLQAACARFGRGRSAEQIIAEARSQ
                     WHPAKPLLELYHRETRRVADGFRQARAVSFPQGDVLQVKPVPEFLRAVIPTAAYQQPA
                     AFTKAQRGVFWVNDLSLTKTTESEKLAEQQQHFGLPLTCAHEAYPGHHLQFVTANHHP
                     RKWRRLFAHAVFYEGWTLWCEQMLADLRIDRSSWLPVQQLHDALWRCHRILVDLRLQT
                     GRYSYEQAVRHLQRHVGFTRARAEADVNWYTAQPAVPMSYWLGRLENERLRQRLMTGR
                     GWSLRRFNGWLLSFGTIPQAWIEKYGLD"
     gene            1840..3579
                     /locus_tag="VIL39_08865"
     CDS             1840..3579
                     /locus_tag="VIL39_08865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY5481575.1"
                     /translation="MDLLPFDGLPAHRPRRFVPQGIDLGDWGQMAPLFDQLEARAQAC
                     ASAADLERWLLDWSELCAAIDEESSKRYIAMTCHTDNAEAEKAYLHFVENVEPQLKPR
                     QFKLAQVYVTHPLRKQLPKERYEVFDRDTQLHVELFRAENVPLETEEARLSQQYQKLS
                     GSLTVQFRGEEKTLVQMGRYLEEPDRALRQEAWELVANRRLQEAEKFEEFFDQLVKLR
                     ERIARNAGYGNYRDYAFRRLGRFDYTPEDCAKFHAAVEAEVMPMVAELQAERQRQLGV
                     QTLRPWDVAVDPLNRPPLRPFEQVDQMVSRTQEIFGCLDPELAAGFQRMQNLHLLDLA
                     NRKGKAPGGYQSTLSEARVPFIFMNAVGLQRDVETILHEAGHAFHALATRDEDLYGYR
                     SAPIEFCEVASMSMELLGNEFLERFYAVPEANRARRTHLEGIVNILPWIATVDAFQHW
                     IYTHPSHTRAERTAAWVALMGRFGGAVDWSGYEAARANLWHRQLHIFIHAFYYIEYGI
                     AQLGALQIWANSKRDKAEALNAYKRALALGGSRPLPELFAAAGCKFEFSRKTMGPLVK
                     LLRAELARLPAEK"
     gene            3576..4496
                     /locus_tag="VIL39_08870"
     CDS             3576..4496
                     /locus_tag="VIL39_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY5481576.1"
                     /translation="MKVLVTGATGFVGREVVRQVHEAGHSIRILARTRNSSRVHEAIS
                     RYGAEVHPGDVLDAASLDGAINGMEAVIHLVGIIAEVGESTFEEVHMHGTGNIVMAAQ
                     QGRARRFVHMSALGTRPNAASRYHQTKWAAEELVRHSGLDFTVFRPSLIYGPQDQFIN
                     LFARIIRLSPVVPLIGSPRARFQPVSVEAVAAAFVGSLGEPRSIGQTYDLCGPEALTL
                     SEVVDQIMGVLQRRRLKFQVPLGLARCQAALLEFTFRRLLRKAFPLSRDQLLMLQEDN
                     VGNPRPANELFGLKPLPLREGIAGYLMRDA"
     gene            complement(4543..4989)
                     /locus_tag="VIL39_08875"
     CDS             complement(4543..4989)
                     /locus_tag="VIL39_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481577.1"
                     /translation="MAAPGADSSRQSAKGKILIVDDEEGMRDLLKAILEADYHIAEAA
                     SGAALHQALEKEEPDVVLLDMKLPDANGLSLLPAIKQRWPATEVIILSGTPNDSRAAS
                     WAAEAVKGGAFAFLSKSAEFSFPQILDCVSSALEHRCQAQVSTPDA"
     gene            complement(4989..6353)
                     /locus_tag="VIL39_08880"
     CDS             complement(4989..6353)
                     /locus_tag="VIL39_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5481578.1"
                     /translation="MKGRILLVDDDAGYRKVLTGFLEDDYEVTEAESGDALKKALTEA
                     QPDVVLLDVKLGRDNGLDLLPSIKRRWPETEVIVVTGAPKDGTEAVSWAVEATKRGAY
                     NFIRRSEQFDGQKLLADVANALGARRQSEETSILRRALETMSGTTSPIFQSAAMREVV
                     RTVERIAPSDVTVLITGESGTGKEVIADLLHAFSPRSKGKIVKINCAALPRELIESEL
                     FGSVKGAFTGAHTDREGLFRQAEGGTLLLDEISEMPIDTQSKLLRVLQDQEVRPVGGK
                     TTYKTNCRIIAATNRKTDEAIAQGKLREDLFYRISAISVHLPPLRDRREDIMPMANAF
                     LKRFSAQANRVIKGFTPSAAERLSSFDWPGNVRQLQNEVQRAVLLSEGEEVDATDLSI
                     SQGKVSGEDSLDTNFTLLEGVERNAIVQMLRETGGNKLETARRLGIGRQTLYNKIKAY
                     GIEV"
     gene            complement(6350..7126)
                     /locus_tag="VIL39_08885"
     CDS             complement(6350..7126)
                     /locus_tag="VIL39_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5481579.1"
                     /translation="MTRPPLILISPNVDKKGDEFGDLSISLSETYQQALLNCGAIPIA
                     LPATISRNVIAECVGHCDGVLLTGGEDVDPRLYGNGLPPRLRGTVTVTPDGGARDLRE
                     LVLIDEVFRQRKPLLGICRGHQMLNVALGGTLVTDILAQRPRALNHRRMDRRSEIVHE
                     ARLTPGSLIAKITGGHRLGVNSTHHQAVARVAGPLKVAAVSDDGIIEGLELRPGAAKM
                     LPFLLSVQFHPERLADRYPEHQAIFRALTRACVLNRKNKL"
     gene            complement(7188..7460)
                     /locus_tag="VIL39_08890"
     CDS             complement(7188..7460)
                     /locus_tag="VIL39_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481580.1"
                     /translation="MAKAEISWKRVTTEGIRLQVYAQHVSREWRFFARERRYDQWQAI
                     ADPPLEDWIELLDSIQRRINRRLLRPEEEARVRKSIRERFPEAKLE"
     gene            complement(7517..7993)
                     /gene="ispF"
                     /locus_tag="VIL39_08895"
     CDS             complement(7517..7993)
                     /gene="ispF"
                     /locus_tag="VIL39_08895"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5481581.1"
                     /translation="MIHVGIGYDVHALVEGRKLILGGVEIPHPKGLDGHSDADVLMHA
                     ICDAVLGALGEADIGHFFPNTEPRWKGARSRIFLNEAARLVSFREGKIINVDATVIAQ
                     APKIFPHVREMKINIAEALGLSIQHVGIKATTNERLGFLGREEGIAAMAVASVDLP"
     gene            complement(8158..8409)
                     /locus_tag="VIL39_08900"
     CDS             complement(8158..8409)
                     /locus_tag="VIL39_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5481582.1"
                     /translation="MSGKPFDETTIEEVWSRAPISSDRPPLRVDAYGAHIWRQGYGNT
                     NSKFGWQIGRKTPLSKGGKDDLENLQPLQWENNRRGSGS"
     gene            complement(8627..9787)
                     /locus_tag="VIL39_08905"
     CDS             complement(8627..9787)
                     /locus_tag="VIL39_08905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY5481583.1"
                     /translation="MLGAFGCLLLVSANQATAAAAKPTVHVVQAPDQCLVPDVVVDIK
                     GVLHMVYGLAHHAYYVRSTDNGAAFTSPVKVNSTGTVETKMGERGPKLAVGTDGSIHV
                     VWVDEWAPGVKTFVRYSRSLDGGKSFEALKTLSAMSGVDGTTLTADGKGHVLAFWHVM
                     VDPKPEVKAATWLHTTRSTNNGAAFGPTEKVNITNLSGLACSMCMMRARTGADGSVYL
                     AFRSAEASIRDFYMLKGHPTENRFTALRVNQDNWNIDYCPMCGPELTLTPNGQSLCAF
                     MTRKRVYWAVGDAGMTGFRMHVATPTSEEDELYPSAVANRNGAILFIWQVGRMAVKGT
                     ATVKWAQYDEDGKPTGDAGTAGQSFAGTKATAFVGDDDNFYIVTTARKTNEP"
BASE COUNT         1803 a         2994 c         3043 g         2022 t
ORIGIN      
        1 acaggtgcgc gttgggcgca aaattactga taccggtgtg aatgccacag gccggagcat
       61 aacatgattt gccgtgtatg tggcaagcgg caattaaacg tcaaccggcg cggggcgcag
      121 ttctgcagaa agccccactt cttgcgtgca agcgggggct tttcgtgcgt tgcttaagcc
      181 atgaatcgga ctgcgaagca attcgagggg ctgctcgccc attactttga agccctgctc
      241 catgacaatc ccacgttttc caccatcggc gccgggctgc gcgacggtga aggcaagctg
      301 ggccgcctga cgctggactt ccagaagagg cgcgagcgcg agcggcaatc tgcgctgcga
      361 gcgttggaag gcatttcgcc gcgcgagctg acgagcgagc agcaacttga ccggctggcg
      421 ttgcgcagcc tgctgctcaa ggagtgcgaa gattacgccc ggggccggca tgcgctcgaa
      481 ccaaacgccc cggatcaatt gctcaacatc ctgctgcacg agctgatgcg cggggacgac
      541 gagccccggc ggggggctcg caacctgcgc tcgctgctcc ggcaagcacc cgatttcctg
      601 gatgaggcgg cggcggtggt ccgcagccct gagcgggtct ggctgcgagt gatggaacaa
      661 accgtggcgg gcggcatgga actacttgag ggcgtgggga agtttctcca gacggttgca
      721 ccgcaggcgg gcgatgcggt gttggtttcc gctgccggga aggcgatgca gcgttaccgc
      781 gcgcgggtga agcagcgttc gttggcgcca gcgggctcgt ttgccattgg agcggcggcg
      841 ttgcaacgcc gggtgcggga tgagcttggt ttggattata cgctcggcca ggtggaagcc
      901 ctggcgctgg gcgaagtgga gcgcgtgggc acgctgctcc aggcggcctg cgcgcgcttc
      961 ggccgcggcc gttcggcgga acagatcatt gccgaggcgc gttctcaatg gcaccccgcc
     1021 aaaccgctgc tggaactcta ccaccgggaa acgcggcgcg tggcggacgg gtttcgccag
     1081 gcgcgcgcgg tgtcgttccc gcagggggac gtgctccagg tcaaaccggt gccggagttt
     1141 ctgcgcgcgg tgattccaac ggccgcgtat cagcagccag cggcgtttac gaaagcgcag
     1201 cgcggggtgt tctgggtaaa cgacctgagc ctcacaaaga ccaccgagtc cgagaagctc
     1261 gccgagcagc agcagcattt cggccttccg ctgacttgcg cccacgaggc ttaccctgga
     1321 catcacctgc aattcgtgac ggccaaccat catccgcgga agtggcgtcg gctgtttgcc
     1381 cacgcggtct tttacgaggg ctggacgctc tggtgcgagc agatgctggc ggacctgagg
     1441 attgaccggt cgtcctggct gccggtgcag caattgcatg acgcgctctg gcgctgtcat
     1501 cgcatcctgg tggacctccg gctgcaaacc ggccgctaca gctatgagca agctgttcgg
     1561 cacctgcaga ggcatgtggg cttcacccgc gcgcgagctg aggccgacgt gaattggtac
     1621 accgcccagc cggcggtgcc gatgagctat tggctgggcc ggctggaaaa cgagcggctg
     1681 cgtcagcgcc tgatgaccgg ccgcggctgg agcctgcgga ggttcaacgg ttggctgctg
     1741 agcttcggga cgatcccgca ggcatggatt gagaagtatg ggttggactg aaatctgaat
     1801 tccagtttcc aaccaggcgg attgaagtat cttccgcgca tggatctact gccgtttgac
     1861 gggttgccgg cgcataggcc ccggcgcttt gtgccacagg ggatcgacct gggagactgg
     1921 ggccagatgg ctccgctctt cgaccaactg gaagcccgcg cgcaagcgtg cgcctccgcc
     1981 gccgacctgg aacggtggct gctggattgg agtgaactct gcgccgcaat agatgaggag
     2041 tcctccaagc gttacatcgc catgacgtgc cacaccgaca atgccgaagc ggaaaaggcc
     2101 tatcttcact tcgtggagaa cgttgagccg caactcaagc cgcgccagtt caagctcgcc
     2161 caggtttatg tcacacatcc gcttcgcaaa cagttgccga aggagcgcta cgaggtgttt
     2221 gaccgtgata cgcagcttca tgtcgaactg ttccgggcgg agaacgtgcc gctggagacc
     2281 gaggaagccc gactgagtca gcagtatcag aagctgagcg gttcgctcac cgtgcagttc
     2341 cgcggcgaag agaagacgct ggtgcagatg ggccgctacc tggaagaacc cgaccgggcg
     2401 ctgcgccagg aagcgtggga attggtcgct aaccggcggt tgcaggaggc ggagaagttc
     2461 gaggagttct ttgaccaact ggtgaagctg cgggagcgga tcgctcgcaa tgccggttat
     2521 gggaactacc gggattatgc gtttcgcagg ctgggccggt ttgactacac accggaggac
     2581 tgcgcgaagt tccacgcggc agtggaggcc gaagtgatgc cgatggttgc cgaactccag
     2641 gctgagcggc agcggcaact cggagttcag acgctccgcc cttgggacgt ggccgtggac
     2701 ccgctgaacc ggccgccgct gcgtccgttc gagcaggtgg atcaaatggt ctcgcgcacg
     2761 caggagattt tcgggtgcct cgatcctgag ctggcagcag gattccagcg gatgcagaat
     2821 ctgcatctgc tcgacctggc caaccgcaaa ggcaaagcgc cgggcggtta tcagtccacg
     2881 ctgtccgaag cgcgggtgcc atttattttc atgaatgccg tcggcctgca gcgggacgta
     2941 gagaccatcc tgcacgaggc cggccatgcc tttcatgcgc tggctacgcg cgacgaggat
     3001 ctctacggct accgcagcgc gccgatcgag ttctgcgaag tggcctcgat gagcatggaa
     3061 ttgctgggca atgagttcct cgaaaggttc tacgccgtgc cggaggccaa tcgcgcccgt
     3121 cgcacgcacc tggaagggat tgtgaacatt ctgccgtgga ttgctacggt ggatgcgttc
     3181 cagcactgga tttatacgca cccaagccac acgcgggcgg agcggaccgc ggcgtgggtg
     3241 gcattgatgg gccggtttgg cggcgcggtg gactggagcg gatacgaggc ggcccgggct
     3301 aatctctggc accgccaatt gcatatcttc atccatgcct tttactacat cgaatacggc
     3361 atcgcccaac tcggcgcctt gcagatctgg gccaactcga agcgggacaa ggccgaggcg
     3421 ttgaatgcct acaagcgagc cctggcgctg ggcggctcgc ggccgttgcc ggaattgttc
     3481 gcggccgccg gctgcaagtt cgagtttagc cggaagacga tggggccgct ggtgaagctg
     3541 ttgcgagccg aactcgccag gctgcccgcg gagaaatgaa agtcctggtc actggagcaa
     3601 cgggattcgt tggccgggaa gttgtccggc aggttcacga ggctgggcac tcgattcgca
     3661 ttctcgcccg cacgcgcaat tcatcgcgcg tgcatgaggc aatttcacgt tacggggccg
     3721 aagttcatcc cggcgacgtg ctggatgccg cttcgctgga cggagcgatc aacgggatgg
     3781 aagctgtgat tcatctcgtg ggcattatcg ctgaagttgg cgagagcacc ttcgaggagg
     3841 tccacatgca cggcacggga aacatcgtga tggcggcgca gcagggaaga gcccggcgct
     3901 tcgtccacat gagcgcgctg ggcacgcggc ccaatgcggc ttcgcgttat caccaaacca
     3961 aatgggccgc tgaagaactc gttcggcaca gcggattgga cttcacggtt tttcgcccct
     4021 cgctcatcta cgggccgcag gaccagttca tcaacttgtt tgcgcggatc attcgcctct
     4081 cgccggtcgt gccgctgatt ggcagcccgc gcgcgaggtt tcaacctgtc tcggttgagg
     4141 ccgtagcggc cgcttttgtt ggctcgcttg gcgaacccag gtcaatcggc cagacctatg
     4201 atttgtgcgg cccggaggcc ttgacgctct cggaagtcgt ggaccagatt atgggagtat
     4261 tgcagcggag gcggttgaaa ttccaggtgc cgctcggcct cgcacgttgc caggccgcgc
     4321 ttctggaatt cactttccgg cgcctgctgc gaaaggcgtt ccccctcagt cgcgaccaac
     4381 tcctcatgtt gcaggaggac aacgtgggaa acccccggcc ggcgaatgag ctctttgggc
     4441 tgaaaccgct gccgcttcgt gaagggattg ctggatactt gatgcgtgat gcgtgatgcg
     4501 tgaaaggaga cggcatggca gcctcgtttc acgcatcacg catcacgcgt caggggtgct
     4561 tacctgggcc tggcagcggt gctccagggc gctgctgaca cagtccagga tttgggggaa
     4621 actgaattcg gcagatttcg agaggaaagc gaaagcgccg cccttgaccg cttcggctgc
     4681 ccaggacgct gcccggctgt cgtttggcgt tccgctcaag ataatgactt cggtcgccgg
     4741 ccaccgctgc ttgatcgccg gcagcaaact gagtccattg gcgtcgggca gtttcatgtc
     4801 gaggagcacc acgtccggct cttccttttc caaggcctga tgcaatgccg cgccgctagc
     4861 cgcctcggct atatggtagt cggcttccag gatggccttg aggagatcac gcatcccctc
     4921 ctcatcatca acgatgagga tcttgccctt cgcggactgc cgtgaggaat ctgcgccggg
     4981 cgcagccatt agacctcaat tccgtaggct ttgatcttgt tgtagagggt ttggcggccg
     5041 atgccgaggc gtctggcggt ttccagtttg ttgccgccgg tttctctaag catctggacg
     5101 atcgcattgc gctcgacccc ttccagcaag gtgaagttgg tgtctagcga gtcttcgccg
     5161 gagaccttcc cctgactgat ggatagatca gtcgcatcca cttcttcgcc ttcggatagc
     5221 agcaccgcgc gctgcacctc gttttgcaac tggcgcacgt tgcccggcca gtcgaaagag
     5281 ctcaaccgct ccgccgcact cggggtgaag cctttaatga cccggttggc ctgggccgag
     5341 aaacgcttca agaaggcatt ggccatcggc atgatgtcct cgcgccggtc gcgcagcggc
     5401 gggagatgga ccgaaatggc gctgatgcgg tagaacaaat cctcgcgcag tttgccttgt
     5461 gcaatcgcct cgtccgtctt gcggttggtt gcggcgatga tgcggcagtt ggtcttgtaa
     5521 gtggtctttc cgcccacggg acgcacctcc tgatcctgca gcacgcgaag tagcttgctt
     5581 tgggtatcta tcggcatttc ggagatctcg tccagcagca acgtgccgcc ttcggcctgc
     5641 cgaaacaacc cctcacggtc ggtatgcgcg ccggtgaatg cgcccttcac ggaaccgaac
     5701 agctcgcttt cgatcagttc gcgcggcagg gcggcgcaat tgattttgac gatcttgcct
     5761 ttgctgcgcg ggctgaacgc atggagtaga tcagcaatca cttccttgcc cgtcccgctc
     5821 tcgccggtaa ttaacacggt gacgtcgctg ggcgcgatac gctcgaccgt gcggacgacc
     5881 tcccgcatcg cggcgctttg gaagatcggc gaggtggtgc cgctcatggt ttccagggcg
     5941 cgccgcaaaa tgctggtttc ctcgctttgg cggcgggccc ccagcgcgtt ggcgacatcg
     6001 gccagaagct tttgtccgtc aaactgctct gaccggcgaa taaagttgta agccccccgc
     6061 ttggttgcct caaccgccca agacaccgcc tcggtgccgt ccttcggcgc accggttacc
     6121 acgatgactt cggtttccgg ccagcgcctc ttgatggagg gcaacaaatc gagtccgttg
     6181 tcacggccta gtttgacgtc caggagcact acatcaggct gtgcttcagt tagggccttc
     6241 ttcagcgcat ccccgctttc agcctcggtc acctcgtagt catcctcaag aaagccggtc
     6301 agcactttgc ggtagcccgc atcgtcatct actaacagaa ttctgccctt cataacttgt
     6361 ttttgcgatt aagcacgcag gcccgcgtca atgcccggaa gatcgcttga tgttccggat
     6421 accggtccgc cagccgctcc gggtgaaact gcacgctcaa caggaacggc agcatcttgg
     6481 ccgcgcccgg ccttaactcc agaccctcaa tgatcccgtc atcgctgacg gcggccactt
     6541 ttaaaggtcc ggccacccgc gcgacagcct ggtgatgtgt gctattcaca cccagtctgt
     6601 gcccacccgt tatcttggct attaacgaac ccggtgtcaa ccgcgcttcg tggacaatct
     6661 cgctgcgccg gtccatgcgg cgatggttca gcgcccgggg tctctgggcc agaatatccg
     6721 tcaccagcgt gccgcccagc gcgacgttca gcatctggtg gccccggcag atccccagca
     6781 gaggtttgcg ctgccggaaa acctcgtcaa tcagcaccaa ttcacgcaaa tcccgcgcgc
     6841 cgccgtcagg tgtcacagtc accgtgccgc gcagacgcgg cggcagcccg ttcccgtaaa
     6901 ggcgtgggtc cacatcttcg ccgccggtca gcaaaacgcc gtcgcagtgg cccacacatt
     6961 cggcgattac gttccgtgag atggtggctg gcagggcgat gggaatggct ccgcagttca
     7021 acagggcctg ttggtaggtc tccgaaaggc tgatcgagag gtcgccgaat tcatcgcctt
     7081 tcttgtcaac gttcggcgaa atcagaatca gcggtggtct ggtcatatcc agggattctg
     7141 aaagaggcgg ggcgctcctg cgtcaccccc ggaacgtctg cccttgttca ttcgagtttc
     7201 gcctcgggaa accgctcacg gatggacttt ctcacgcgcg cctcttcctc gggccgaagc
     7261 aaacggcgat tgatgcggcg ctggatcgag tcgagcagtt cgatccagtc ctccagtggc
     7321 gggtcggcga tggcctgcca ttggtcgtaa cgccgctctc gcgcgaagaa ccgccactcg
     7381 cggcttacgt gctgcgcata cacctgaagc cggattcctt ccgtcgtgac tcgtttccaa
     7441 ctgatttcgg ctttggccat ggcaattgtt caaaccgcgc cagccgtcga ggctgggact
     7501 gtgggctggg ggtgggtcat ggcagatcaa cgctggccac ggccatggct gcgatgcctt
     7561 cctcccggcc caggaatccc aagcgttcgt tggtggtcgc cttgatcccg acatgctgga
     7621 tgctcagccc gagggcttca gcgatgttga tcttcatctc gcgaacatgc gggaagatct
     7681 tgggcgcttg ggcaatcacc gtggcatcca cattgatgat cttgccttcg cgaaaactga
     7741 ccagccgcgc ggcttcattc aagaaaatac ggctccgtgc gcccttccaa cgcggttctg
     7801 tgttcggaaa gaagtggccg atatccgcct cgcccagcgc gccgagaacc gcatcgcaaa
     7861 tcgcatgcat caacacatcg gcatccgaat gcccatccag acctttcggg tgcggaatct
     7921 ccacgccacc caggatgagt tttcgcccct cgaccaaagc gtgcacatca taaccgatgc
     7981 caacatgaat catgcgaagg attatagacc catcccctgc cgcaaccaag gaatttcagg
     8041 ccggcagcaa tgccttctcc gacgacacaa cggaagagcg gcttggcttt tacgccgacc
     8101 ctcgctaata gtcttcgccg ttgaactcgg cgtctgcccc ggcccatcca gtcaggctta
     8161 actgccgctg ccgcgccggt tgttttccca ttgcagcggc tgaaggttct ccagatcatc
     8221 tttcccgccc ttggacaaag gggtcttgcg cccgatttgc cagccaaatt tactgttcgt
     8281 gttcccgtag ccctgtcgcc aaatgtgcgc gccatacgca tcaactctca gtggcgggcg
     8341 gtcgctgcta atcggcgccc tgctccatac ttcttcaatg gttgtttcat cgaagggctt
     8401 gccggacatg tctgtactcg gttgtctgaa catattatcg ctcggttcac ccgttgaccg
     8461 gatgcccgta aaaggctggc cactgtcatg gcgcactctt gagtcctaaa cgctacgaca
     8521 attcagccgt gtctcctttg agcctcggga gcgctattcc gttcaaaaga agataaaaca
     8581 ccgcaattcg tctccagccc ggttgtcggg ctcctttcaa attgattcag ggttcgtttg
     8641 ttttgcgggc agtcgtgacg atgtagaagt tgtcgtcgtc ccctacgaat gctgtcgcct
     8701 tcgttcccgc gaacgactgg cctgcagttc cggcgtcacc ggtgggcttt ccgtcctcgt
     8761 cgtactgtgc ccatttgacc gtggcagtgc ctttgacggc catccgtccc acctgccaga
     8821 taaacagaat ggccccgttg cggttggcca cagcggacgg gtaaagctcg tcttcctcgc
     8881 tggtgggcgt ggctacgtgc atccgaaatc cggtcatacc ggcatcgcca acagcccaat
     8941 aaacccgttt ccgggtcata aacgcgcaca gggactggcc attcggcgtg agcgtcaact
     9001 ccgggccgca catggggcag tagtcaatgt tccagttgtc ctggttaacc cgcagcgccg
     9061 tgaaccggtt ctcagtgggg tgccctttga gcatgtagaa gtcgcggatg ctggcctctg
     9121 cgctgcggaa cgccagatac acggagccgt ctgcccccgt ccgcgcccgc atcatgcaca
     9181 tggaacaggc cagccccgac aagttggtga tgttcacttt ctctgtcggc ccgaaggccg
     9241 cgccgttatt ggtcgagcga gtggtatgga gccacgtcgc cgctttgacc tcaggcttcg
     9301 ggtcaaccat cacgtgccaa aacgcaagga catggccttt gccatcagcc gtcagtgtgg
     9361 tcccgtcaac gcccgacata gcggaaaggg tctttagcgc ttcgaacgac ttgcccccgt
     9421 cgagactgcg ggaatagcgc acgaacgttt ttacgcccgg tgcccattca tccacccaga
     9481 cgacatggat gcttccatct gtgccgacgg ccagctttgg gccgcgttcg cccatcttcg
     9541 tctccaccgt gccggtggaa ttcaccttca caggcgaggt gaatgcagct ccgttgtcgg
     9601 tagagcggac gtagtaggcg tggtgtgcca gaccgtaaac catgtggagg acacctttga
     9661 tatccacgac cacatcgggg accagacact ggtcgggcgc ctgcactaca tgcacggtcg
     9721 gcttcgctgc cgccgcagtc gcttgattgg cgcttacaag gagcaagcag ccaaatgcgc
     9781 cgagcatcaa gctggtattt acccgtggaa gacggcctgg ccacagtggc aggcactcta
     9841 ccgcagttgg ctccggcgtt ct
//
LOCUS       DATCGS010000430         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_483092, whole genome shotgun sequence.
ACCESSION   DATCGS010000430 DATCGS010000000
VERSION     DATCGS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483092"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VIL39_08910"
     CDS             complement(<1..272)
                     /locus_tag="VIL39_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY5481584.1"
                     /translation="MSEINARPLLDWLLRKHPDTPKNRAKQWILAGRVSVKGVVIRKP
                     HQTIADPGDTLELRARNATTLACGSGWQIHPRVSLLYLDSALAIVN"
     gene            complement(614..2566)
                     /locus_tag="VIL39_08915"
     CDS             complement(614..2566)
                     /locus_tag="VIL39_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481585.1"
                     /translation="MRKKVLLTSVCRPMGPKYGDAPSVGYELLYGQVTRAQGLFSPRT
                     VNIHYSLEYIAENLDAPTVVLQYPSERELIRELKKGYDYVGVSFLLAVMHKMKRVVEL
                     IRQYAPSSKIVLGGYGTVLKDEELKPYGDYFCREEGVGFFRRLLGEPEIPMPYKQPLL
                     VSWLKIFGQKVSATGQIFAGLGCPNGCDFCCTSHFFSRKHIKLLPEGKDIYAVAERYL
                     DMNPELVLLIIDEDFLLNKKRAMEFRDCVMKGGKTLSIFAFSSVRALSQYKVEEILEM
                     GIDGFWIGYEGTRSGYGKQQGRRIEDIITEFREHGITILTSMIVGFDYQTPEVVAQEL
                     GGLMKLKPALAQFLIYGPVPGTPFHERVMKDNLLQDVYTNDKDLYYRRGDGFATMVKH
                     PTLSPAAIEDLQRWCFNEDFQRLGPSIFRTVEAWLLGYQKLKNSPNRFLRQKAEFYAK
                     EMRAAYPAFLAGKLLGPNTAVRRWTGELEKHVRAELGRPTFAERFRSVLGVGAALWTG
                     LTLKLNLFQHPKLMRTMYRMPAKRWAGFDLWEELHRKTVFPNLSIQVELQHAKHQVWM
                     RLEGRLSRAEAEGLGQRIRDSLARSKSRLVLDLKKVQWDKVDDLRPLREKLAAYRSRI
                     RLILPKVTAAHPEVILLASMFEHYRG"
     gene            2559..2849
                     /locus_tag="VIL39_08920"
     CDS             2559..2849
                     /locus_tag="VIL39_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481586.1"
                     /translation="MRIDNYISLSKILRRVAYPVEPFGFEYIKGELPPPLLQGNRDRM
                     GKQPGLFEQANRRLANKLQYMHPDRCYNSRQTVPSFFESSIAKSQAATTSAS"
     gene            3116..>3304
                     /locus_tag="VIL39_08925"
     CDS             3116..>3304
                     /locus_tag="VIL39_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481587.1"
                     /translation="MPGTAVPGTPPGLWGSQHAVWPSPATEPDEPSQLPGRQETKARD
                     TPGTSQATAMDSLLFLYLS"
BASE COUNT          648 a         1003 c          957 g          696 t
ORIGIN      
        1 ttgttgacga tcgcgagggc ggaatccagg taaagcaacg aaacgcgcgg atgaatctgc
       61 caaccggaac cacacgccag cgtggtggcg ttgcgcgcgc gcagttcgag tgtgtcgccg
      121 gggtcggcga tggtttggtg gggtttgcga atgaccacgc ccttgacact cacgcgcccc
      181 gccaggatcc attgtttggc gcggttcttc ggtgtgtcgg gatgcttccg cagcaaccaa
      241 tccaacaggg gcctggcgtt tatttcgctc actggttcgg gcttccggca tctcttgcca
      301 gtcgcgcggc cctcgacctt gagttccgag ccatcggcac cggcagagac gggctcactt
      361 caaatctgat ttccggttgc gcagccgggc caaggtggag cgggtagcca tttcccggcg
      421 acgctgatct gagagttgat cggagtggaa ttggcggttt tccagctctc ccggtttgag
      481 cttagacttt ttggatctcg gcggacgata tggcatgcct gcaagatggc acacacgcca
      541 ggcacatgcg agcaagaacc tctggccgag gttcgacgcg gccaggtcaa ttccgttgtt
      601 cgtccgcagc aggtcagccc cggtaatgtt caaacatgct ggccagcagg atcacctcgg
      661 gatgcgccgc tgtcactttg ggcaagatga ggcggatgcg cgagcgatag gcggccagct
      721 tttctctaag cggacgcagg tcgtccacct tgtcccactg gaccttcttt aaatccagca
      781 ccaggcggct cttgctgcgc gccagggaat cacggattcg ctgccccaga ccctcggcct
      841 ccgctcgtga cagccggcct tccagcctca tccacacctg atgtttggcg tgttgcaact
      901 cgacctgaat ggagagattg gggaacacag tcttgcgatg gagttcttcc cacagatcaa
      961 atccggccca gcgcttggct ggcatccgat acatcgttcg catcaacttg ggatgctgaa
     1021 aaaggttcag cttcagcgtc aaccccgtcc acagcgccgc gccgacaccc agcaccgatc
     1081 tgaaccgctc cgcgaacgtt ggacgaccca gctccgcgcg gacgtgcttt tccagctcac
     1141 cggtccaacg ccgaactgcc gtgttagggc cgagcagctt ccccgcaaga aacgccggat
     1201 acgctgccct catctccttg gcgtaaaact cggctttctg gcgcaagaag cggttcggtg
     1261 agttcttgag cttctggtag ccgagcagcc acgcttccac cgtgcggaaa atgctcgggc
     1321 cgagccgctg gaaatcctcg ttaaagcacc agcgttggag gtcctcgatg gccgcgggcg
     1381 agagcgtcgg gtgtttgacc atcgtggcga acccgtcgcc ccgccggtaa tagaggtctt
     1441 tgtcgttcgt atagacatcc tgcaacaggt tgtccttcat gacccgctcg tggaacgggg
     1501 tgcccgggac cgggccatag atcaggaact gagccagggc cggcttgagt ttcatcaggc
     1561 cgcccaactc ctgcgctacc acctccgggg tctggtaatc gaatccgacg atcatcgagg
     1621 tcagaatcgt gatgccatgc tcgcgaaact ccgtgatgat gtcctcgatc cgccggccct
     1681 gctgcttgcc gtatccggag cgcgtgcctt cgtagccgat ccagaagccg tcaatcccca
     1741 tctccagaat ctcctccacc ttgtattggc tgagggctct gacgctggag aaggcgaaga
     1801 tagacagcgt cttcccgcct ttcatcacgc agtcgcggaa ctccatcgcg cgcttcttgt
     1861 tgagcaggaa atcctcatca atgatgagca gaaccagttc agggttcatg tcgaggtaac
     1921 gctcggcgac ggcgtagatg tccttgccct cgggcagcag cttaatatgt ttgcgcgaga
     1981 agaagtgcga ggtgcagcag aaatcgcagc cattcgggca gcccagcccc gcgaagattt
     2041 ggcctgtggc cgagaccttc tggccaaata tcttgagcca gcttaccagc agcggctgct
     2101 tgtatggcat cgggatttcg ggctcgccca acagccggcg gaagaacccc accccctcct
     2161 cccgacagaa gtaatcgccg tagggcttga gttcctcgtc cttgagcacg gtgccgtagc
     2221 cgcccaggac gatcttgcta ctgggcgcgt attggcggat caactcgacc acccgtttca
     2281 tcttgtgcat gaccgccagc aggaaagaga cgcccacgta atcgtagccc ttcttgagtt
     2341 cgcggatcag ctcacgctct gaaggatact gcaacaccac cgttggggcg tcgaggttct
     2401 cggcgatgta ctcgagggaa taatggatgt tgaccgtgcg cgggctgaaa agaccctggg
     2461 cgcgcgtcac ctgcccgtaa aggagttcat agccgacgga aggcgcatcg ccatatttcg
     2521 gccccatcgg gcggcacaca ctggttaaga ggactttctt gcgcatagac aattacattt
     2581 cactttccaa gatacttcga cgggttgcgt atccggtcga gccgttcggc ttcgagtaca
     2641 ttaagggcga actgccgccg cctctgctgc agggcaaccg cgacagaatg gggaagcagc
     2701 caggcctgtt tgaacaggcc aaccgtcgtc tggcaaataa gcttcaatac atgcatcctg
     2761 atcggtgtta caattctcgt caaacagttc cttcattctt tgaaagcagc atcgccaagt
     2821 cgcaggcagc gacaacaagc gcgtcctgaa aagcccctca atgcataccg gcttcacaaa
     2881 tctggcttta agcaagattt gcccgcagta ctgcttttgg ccagttcaaa cgtggcctct
     2941 ggggtgaaat ggggccaatg ctttgttcgt atccgggtga tagcttggag ctatgagcgc
     3001 aagtggcggt ggccaatgct tcgtgcgtag catcagccga ttcgtctgcc tcggtcagtt
     3061 aacaaccttg ctcggtgccg gaatctcgtc tgttttccct gcctgggcat cgcccttgcc
     3121 gggcacggct gtgccgggca cgccacccgg cctgtgggga agccagcacg ccgtctggcc
     3181 atctcccgca accgagccgg acgagccgtc gcaattgccg ggccggcagg aaaccaaagc
     3241 ccgggacacg cctggaacca gccaggcgac cgcgatggac tctctattgt ttctctattt
     3301 gtcc
//
LOCUS       DATCGS010000431         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_86493, whole genome shotgun sequence.
ACCESSION   DATCGS010000431 DATCGS010000000
VERSION     DATCGS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86493"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VIL39_08930"
     CDS             complement(<1..1109)
                     /locus_tag="VIL39_08930"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY5481588.1"
                     /translation="MGWRRVLTIGILGLALLGTGSASALDEVPYGVAQEPWTEGMGNH
                     RAIVRVEQKADAVLVKIPWRRRDLDPERKQIILVDSATSQRITNLARLRLDRFEGVLA
                     FQPQTVPGDYFVYYVPFVPEPNWGNYNHDYLPPQETAAADWKTRLPADPEGLPRAAVV
                     RLEARTEFDSFYPMEVVATPEETQAMLKRSAESAYLLFPEDRRFPIRMTDELPLRWVK
                     SGPGKEFHGEVQRNEFYVFQVGVWAARTNLAGLDVEFKGEIARWLNCFNTGGTNWDGK
                     PFRKTVNVPQGRVQALWLGVNVPHDAKPGEHRATVTIHPTNALPGVVELKLNVLPTEL
                     ADHGDSEPWRLARLRWLDSTLGADDSVVSPYEPIQT"
     gene            complement(1163..2218)
                     /locus_tag="VIL39_08935"
     CDS             complement(1163..2218)
                     /locus_tag="VIL39_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY5481589.1"
                     /translation="MSAEANTRRQFASDNYAGICPEAFAAMVEANQGHEVSYGNDIWT
                     DKASKLIRDVFETNCEVFFVFNGTSANSLSLASLCQSYHSILCHEQAHIETSECGAPE
                     FFANGTKLLLLPGANGKVDPKAIDNAVNKRTDIHHSKPRVLSLTQATEVGTVYSLDEL
                     RAVTDAARRYHLRVQMDGARFANAVVALGATPKEATWKAGVDVLCFGGSKNGIALGEA
                     VVFFNTELAREFDYRCKQGGQLASKMRFLSAPWVGMLQDGAWLRYGKHTNAMARRLES
                     AIRHLPHVKIAHPVQTNSVFARLPDDVVQGMHQRGWKFYTHVSQDNARLMCSWDTTAE
                     DVDAFAADLAEVTAHCG"
BASE COUNT          424 a          760 c          696 g          446 t
ORIGIN      
        1 gtttggatag gttcgtaagg agagacgacc gagtcatcgg ctccgagagt cgaatccagc
       61 caccgcagcc gcgcgagccg ccacggttcg ctgtccccgt ggtccgcaag ctcggtcggc
      121 agcacgttga gctttagctc tacaactcca ggcaacgcgt tcgtcgggtg aatggtaacc
      181 gtcgcgcggt gctcgcccgg ctttgcgtcg tgcggcacgt tcaccccgag ccacagtgcc
      241 tgcaccctgc cctgcggcac gttgaccgtc ttgcggaaag gcttgccatc ccagttggtg
      301 ccgccggtgt tgaagcaatt cagccatctg gcaatctcgc ccttgaactc gacgtccagc
      361 cccgcgagat ttgtccgcgc cgcccagacg ccgacctgga atacgtagaa ctcattccgc
      421 tgcacctcgc cgtggaattc cttgccggga ccggacttca cccaccgcag cggcagctca
      481 tccgtcatgc ggatggggaa acggcggtct tcggggaaga ggaggtatgc ggactccgcg
      541 ctccgcttca gcattgcttg cgtctcttcc ggcgtggcga ccacttccat cgggtaaaag
      601 ctgtcgaact ccgttcgggc ttccagccga acgactgcgg cgcgcggcag cccctccggg
      661 tccgccggca accgtgtctt ccagtcggca gcggcagtct cctgcggcgg cagataatcg
      721 tgattgtagt tgccccagtt cggctccggc acaaagggca cgtaatagac gaagtaatcg
      781 cccggcaccg tttgcggctg gaaagccagc acgccctcaa accggtcaag gcgcagccgc
      841 gcgaggttgg tgatgcgctg gcttgtagcg ctgtccacga gaatgatttg cttgcgctcg
      901 gggtcgaggt cccgccgccg ccagggaatc ttgaccagga cggcatccgc cttctgctcg
      961 acgcgcacga tggcccggtg attccccatg ccttccgtcc acggttcctg cgccacgccg
     1021 taggggactt cgtccaaagc ggaagccgag ccagtgccca gcagggcgag accaaggatg
     1081 cctatggtca ggactcttcg ccatcccatg ggaccaatgt gacttatgta acccatgcgc
     1141 cccctgctta ccgctcatac cctcagccgc aatgggccgt gacctcggcc aaatccgccg
     1201 caaacgcatc cacatcctca gccgtcgtat cccaactgca catgagccgg gcattgtcct
     1261 ggctgacgtg cgtgtagaac ttccacccgc gctggtgcat gccttgcaca acgtcatccg
     1321 gcagccgggc gaataccgaa ttggtctgga cggggtgcgc gatcttcacg tgaggcaagt
     1381 ggcgaattgc cgattccagc cgccgtgcca ttgcgttggt gtgcttgccg taccgcagcc
     1441 aggcgccatc ctgcagcatc ccgacccacg gcgcggagag gaaccgcatc ttcgaggcga
     1501 gctgcccgcc ctgcttgcag cgataatcaa actcccgcgc cagttcagta ttgaagaaca
     1561 ccactgcctc acccagcgcg atcccgttct tcgagccgcc gaagcagagc acatccaccc
     1621 ctgctttcca ggtggcttcc ttgggcgtag cccccagcgc gacgacggca ttggcaaagc
     1681 gcgccccgtc catctgcacc cgcagatgat agcgccgggc ggcatcggtc accgcccgca
     1741 gctcgtcgag cgaatacacc gtgcctacct cggtcgcctg cgtcaggctg agcacgcgcg
     1801 gcttggaatg atgaatatct gttcgcttgt tcaccgcgtt atcaatggcc ttcgggtcaa
     1861 ctttcccgtt cgcgcccggc agcagcagga gcttggtgcc attggcgaag aactccggcg
     1921 cgccgcactc ggatgtttca atatgcgcct gctcgtggca cagaatgctg tggtaggatt
     1981 ggcacagcga ggccagcgag agcgaattgg ccgacgtgcc gttgaagaca aagaacacct
     2041 cgcagttggt ctcgaacacg tcgcggatca gtttggacgc tttatccgtc cagatgtcat
     2101 tgccatagct gacttcgtgg ccctggttgg cctcgaccat cgcggcaaat gcttccgggc
     2161 aaatcccggc ataattgtca ctggcaaact ggcggcgggt attggcttca gcactcatat
     2221 ctgcatctta gagaaacggc tgccggaggg aagcagagaa ttggggccag ggccaattca
     2281 tgaaggggcg gggacggcct cgtccccggt tgttggcggg gcgaag
//
LOCUS       DATCGS010000432         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_86552, whole genome shotgun sequence.
ACCESSION   DATCGS010000432 DATCGS010000000
VERSION     DATCGS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86552"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VIL39_08940"
     CDS             <1..1793
                     /locus_tag="VIL39_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481590.1"
                     /translation="LEYREGRGLVLFCQMDVTARTESEPAAETLAGNILRYVDAWRPA
                     PRRTANYAGEPAGLAWLAACGVKAASLKSEPPTADQVLIVGPGGGRALAAQAPAIGAW
                     VKAGGHVLALGLDEAEANSFLPTKVTMQPKEHIAAWFPPFNALSLLAGVAPADMHNRD
                     PRKLPLVVGGATAYGDGVLAATGNVVFCQLPPFQVSKTMGALSSLAVTEGDSAEHKKS
                     TLVSLGSLVSAQFGQKLPAGEIGKTYTFAASVKALGGPAVVRLEVERAGQPWDRAARG
                     PDITVSPGAWTELHLTFPVDKAYPEGWQAYLNGSGDGARFQIQRMRLYEGPYIPKDDP
                     ASAATEAPDRNLFKNVNFEAGLASWYFNHGPEQFNLRRTFGRTSFAVSRLLGNLGVSG
                     STPLLERFGDPVGASQGPSLIKNGDFSIDTNGDGLADDWEFGANGKAGSCTREKLAGP
                     GSGWAQLITVPPVAAGAQPPEIMIAQHELPIRGAQWYRLSLRTKAEGLTAKDVTWTVQ
                     NTGNWQALFDYQNFATKAEWQTNSFVVQAKDTAARKTKFQIWFTGTGKLWLADVRLEP
                     IQDPTIGRWLDGLYLTRPTEWDDPYRFFGW"
     gene            1938..>2491
                     /locus_tag="VIL39_08945"
     CDS             1938..>2491
                     /locus_tag="VIL39_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481591.1"
                     /translation="MERCVAMLALLLAAHGVVHAQNAARWSWLEPQAKVLPTGDLEWA
                     PKAFEFKTGESVRYIDFESGSDTNDGLTKQTPWKHHPWDPNAGAKAAACKGVQTYVFK
                     QGVDYRGKLNANESGAADAPIILTRDPSWGEGPAVICGAEAVTGWKQGADNNLIPEPE
                     KVWCVDLDWAPRNVWMVGKDGLVTR"
BASE COUNT          463 a          808 c          836 g          384 t
ORIGIN      
        1 tgctcgaata ccgcgagggc cgcgggctcg tccttttctg ccaaatggac gtgaccgcgc
       61 ggacggagag cgagccggca gccgagacac tggcgggcaa catcctccgg tatgtggatg
      121 cctggcgccc agccccccgg cgcaccgcca attacgccgg cgaacccgcc ggcctggcct
      181 ggctggcagc ctgcggggtc aaagccgcct cattaaaaag cgagccgccc acggcggatc
      241 aggtgctgat cgtgggaccg ggtggaggcc gcgcgctcgc agctcaggct cccgccatcg
      301 gtgcgtgggt gaaggcggga ggccacgtcc tggccctcgg actggacgag gcggaggcga
      361 actccttcct gccgacgaag gtcacgatgc agcctaagga gcatatcgct gcctggttcc
      421 cccctttcaa cgccctttcg ctgctggccg gggtagcacc ggcggacatg cacaaccgcg
      481 acccgcgcaa gctgcctctg gtcgtggggg gcgcgaccgc ctatggcgac ggggtgctgg
      541 cagcgacggg caacgtcgtc ttctgccaac tgccgccgtt ccaagtgagc aagacgatgg
      601 gggcgctgag ctcgctcgcg gtgaccgagg gcgactcggc cgagcacaag aagagcaccc
      661 tggtttcgtt gggcagcctc gtctctgccc agttcgggca gaagctgccg gcaggcgaga
      721 tcggtaagac atacaccttc gcggccagcg tgaaggccct cggcgggccg gcggtggtcc
      781 ggctcgaggt cgagcgtgcg ggccaaccgt gggatcgggc ggcgcgcgga ccggacatca
      841 cggtcagccc aggcgcttgg accgagctgc acctcacgtt cccagtggac aaggcctatc
      901 ccgaaggctg gcaggcttac ctgaatggct cgggcgacgg tgccaggttc cagatccagc
      961 ggatgcggct gtacgagggt ccatacatcc cgaaggacga ccccgcatcg gcggccaccg
     1021 aggcgccgga tcgcaacctc ttcaagaacg tgaacttcga ggctggcctg gcatcgtggt
     1081 atttcaacca cggcccggaa caattcaacc tgcgccgcac tttcgggcgc acgtctttcg
     1141 cggtgagccg gctgctgggg aacctcggcg tgtccggctc gacgccgctg ttggagaggt
     1201 ttggtgaccc ggttggtgcc tcgcagggac cttcgctcat caagaacgga gatttctcga
     1261 tcgatacgaa cggcgacggg ctggccgacg attgggagtt cggtgcgaac ggcaaggccg
     1321 gctcctgcac gcgggaaaag ctggccgggc cgggaagcgg ctgggcccaa ctcatcacgg
     1381 tgccgcccgt cgctgccggc gcccagccgc cggaaatcat gatcgcccag cacgaactcc
     1441 ccatccgtgg cgcgcagtgg tatcggctgt cgctgcgaac caaagccgag ggcctgaccg
     1501 ccaaagacgt cacatggacc gtgcagaata cgggcaactg gcaagcgttg ttcgattacc
     1561 agaacttcgc gaccaaggca gaatggcaga cgaactcctt cgtggtccaa gccaaagaca
     1621 cggccgccag gaagaccaag ttccagatct ggttcaccgg caccggcaaa ctgtggctcg
     1681 ccgatgtgcg ccttgagccg atccaggacc cgaccatcgg ccgctggctc gatggcctgt
     1741 acctcacccg acctaccgag tgggacgacc cctatcgttt cttcgggtgg tgagccgcag
     1801 caaaccgttg gagtgatacc acacgccaac gcctgcggcg ggccaaaacc gccaagaacc
     1861 ggcaaaacgt gccgctcgac attgtcttta gcgctcggca gactgcacgc atgatgagtg
     1921 tgagcgcact tcataagatg gaacggtgcg ttgcaatgct ggccctgctg ctcgcagcgc
     1981 acggcgtcgt ccatgctcag aatgcggccc ggtggtcctg gctggagcca caggccaagg
     2041 tgttgccgac cggcgatctg gagtgggcgc ctaaggcctt cgagttcaag acgggcgagt
     2101 cggtccgcta cattgacttc gagtccggca gcgacaccaa cgacggcctc accaaacaga
     2161 cgccctggaa gcaccacccc tgggacccca acgccggcgc caaggcagcg gcctgcaaag
     2221 gcgtgcagac ctatgtcttc aagcagggcg ttgactaccg cggcaagctg aacgcaaatg
     2281 agtcgggcgc cgcggacgcc cccatcatcc tgacccggga cccgtcctgg ggcgaagggc
     2341 cggcggtcat ctgcggcgcg gaggcggtga cgggctggaa acagggcgcc gacaacaacc
     2401 tcatccccga gccggagaag gtctggtgcg tggacctcga ttgggcgcca cgcaacgttt
     2461 ggatggttgg caaggacggc ttggtgacgc g
//
LOCUS       DATCGS010000433         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_581666, whole genome shotgun sequence.
ACCESSION   DATCGS010000433 DATCGS010000000
VERSION     DATCGS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581666"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VIL39_08950"
     CDS             <1..240
                     /locus_tag="VIL39_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEY5481592.1"
                     /translation="ETIEEAITNGREALLDCLEVFKESGRKIPKPVIEAAQWRQRLPR
                     TLYSKLTKQAETEGVSINSLVTAIIAEAIGAKHAR"
     gene            complement(362..1048)
                     /locus_tag="VIL39_08955"
                     /pseudo
     CDS             complement(362..1048)
                     /locus_tag="VIL39_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1294..1434
                     /locus_tag="VIL39_08960"
     CDS             1294..1434
                     /locus_tag="VIL39_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481593.1"
                     /translation="MAAFDSNRVLVLSPQAAGLQNSAFQNAPTAPQDLTALALRWLTP
                     VA"
     gene            complement(1482..2093)
                     /locus_tag="VIL39_08965"
     CDS             complement(1482..2093)
                     /locus_tag="VIL39_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481594.1"
                     /translation="MNTAIEAVVFSSTIQPLAGVTLACVSMIPSVLAAAILARWPQRL
                     AADRATMPAFGGLGWSWRLMAAWLAFPVFYFIFGSIIAPLVLDAYRQGIAGLRIPSFA
                     ETLTTQLVRSPILLAVVLPCVLLGDGSRRKLGFQLGLALAILVGAYGLVQATFFPVHL
                     RIIHTLEIAADEVAYAFVLAWLFRPAESKAAAPFALGVEPTSL"
     gene            complement(2130..2342)
                     /locus_tag="VIL39_08970"
     CDS             complement(2130..2342)
                     /locus_tag="VIL39_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481595.1"
                     /translation="MKNVVLAAVAWLAGTMFSGALASALHLPLPSFPQNTSPAEGFAW
                     FVLASLLLAAGTAPAAASLRGGFKTP"
     gene            complement(2339..2896)
                     /locus_tag="VIL39_08975"
     CDS             complement(2339..2896)
                     /locus_tag="VIL39_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5481596.1"
                     /translation="MKVPEATVLGYALMGLLHQKAQSGYELRKVFATTPIGSFSDSPG
                     SIYPALARLEKRRWIRAAGETGDNPRQRKRCSLTPAGLAELRRWLALPVTCQEVSSST
                     DRFWLRCAFVQQVLGIDETLRFLREADRETTTYLAGLREYQKKFMTSVEFNSGQFAFE
                     CGLAGVAVSLRGIRRAILQIERNPL"
BASE COUNT          576 a          916 c          990 g          500 t
ORIGIN      
        1 gagaccattg aagaagcgat caccaatggc cgcgaagccc tcctcgattg tttggaggtg
       61 tttaaggagt cgggccgaaa gataccaaaa cccgtcatcg aagccgctca atggcgccag
      121 cgcctgcccc gaaccctcta ctcaaagcta acgaaacagg ccgaaaccga gggcgtcagt
      181 atcaatagtc tggtgacggc aatcatcgcc gaagcgatcg gagcaaagca cgcgcgttga
      241 caggagtctg taggagcctt ccggaaccgc cctttccgtg ttggactatg aaataccggc
      301 tcccgtcgtg gacacgtttc gaacttttgc ggtctgcccg ccaacgtact actctcgtgc
      361 gctacttgat ggccacggtc actcctggct gagttacgta atggcccatg ccaacaagga
      421 tgggaacgtc tccaccagtc ggcgcgccgg gcgggaccac gatattcaac tgcatcagtc
      481 cgttggtgga ctgtggggcc acaccggcgt ataggatctg cgcggggaca tggccgaccc
      541 acacttccac cccggcgagc ggcttgggcg gcgtgcctgc cacgatggcg ccatccacac
      601 cggcgggagt gacctgtccg tggccggtga gaaagaggac gagcaccgtg ccgcgggcgg
      661 ccggattggc ggcggaattg aacgtgttgt cctggttgag ggcggcggct tgcccggcgc
      721 cgctggaatc ggcggtaaag atgccaggcg tggtatcggt gacggcgacc tgcaggacgg
      781 gcgaggactt tccctggtag gccacctgaa cgttggttgt gacggtgctg ctcttgaggc
      841 cgaaggggac gatcgcactt acctgtcccg cctgactata tatgagcggc gcggcgactc
      901 cgtcgaaggg gactgtggtt ccggcgaggg ctgtggggat tttgccggtt gcgtcggggc
      961 tgaaataggc taacgtggcg ggccccattt gggtaccgaa cagggtgacg atctcgccgg
     1021 gggcgacggt gacgccggag taggatgcgg cgttgacgac ggatcgaacc gcggggctgt
     1081 cgggcgtggc gggggtgagg gccggctttt ggtgtccgga agaagctgaa gccgttcagc
     1141 agcgtcgccc tattcgaaga ccagggccgc tccaatccgg tgccggatga agacgacccg
     1201 gctggctcac tggtcgacgc tggggtgctg tcgcgatatg tttgcctcgg ggctcagcgg
     1261 gtagcctgct atcccggtca cacggttacg gtcatggcgg cattcgactc caaccgcgtc
     1321 ctggtcctct ccccgcaagc ggcgggattg cagaactcgg cgttccagaa cgcacccacg
     1381 gccccgcaag accttaccgc cctcgcactg cgctggctca cgcccgtcgc ctgaacgcca
     1441 ggcaagggct tcgcccgctt cgcacagtcg cgcagcggcc actacaacga cgttggctct
     1501 acgcccagcg cgaaaggcgc cgcggccttg gattcggcag gccggaagag ccacgccagg
     1561 acgaacgcat aggccacttc gtcggccgcg atctcgagcg tgtggatgat cctcaggtgc
     1621 acggggaaga acgtggcctg caccagtcca tacgcgccca ccagaatcgc cagcgcgaga
     1681 cccaactgga acccgagctt gcgcctcgaa ccgtccccaa gcaggacgca aggcaacacc
     1741 accgccagca agatcgggct gcgcaccagt tgcgtcgtga gcgtctcggc aaacgacggg
     1801 atgcggagtc cggcgatccc ctgcctgtaa gcgtccagga caaggggagc gatgatcgag
     1861 ccgaagatga agtaaaagac gggaaaggcc agccaggccg ccatcagccg ccaactccag
     1921 cccaggccgc cgaacgcagg catcgtggcc cgatcggccg caaggcgctg cggccagcgg
     1981 gcaagaatcg cggcggccag cacggaggga atcatgctca cgcaagccag cgtgacgcct
     2041 gcgagcggct gaatcgtgct gctgaagact acggcttcga tggccgtgtt cacgccgatg
     2101 cagatgaaca gcagtgcgct cagcgccagt cagggggtct tgaatccgcc gcgcaggctg
     2161 gcggcggccg gcgccgtgcc ggcggccagc agaaggctcg ccaatacaaa ccacgcgaag
     2221 ccttcggcag ggctggtgtt ttgcggaaac gaaggtagcg ggagatgcag ggcgctggcc
     2281 agcgctccgc tgaacatggt tccggcgagc cacgcgacgg cggcaagaac gacgttcttc
     2341 ataacgggtt cctttctatc tgtaggatgg cgcggcgaat gccgcgcaga ctgacggcca
     2401 ctccggccag cccgcattcg aaagcgaact ggccggaatt gaattcaaca ctggtcatga
     2461 atttcttttg atactcgcgc aggccggcca ggtacgtggt cgtttcccgg tcggcctcgc
     2521 gcagaaagcg cagtgtctcg tcaatgccga gaacctgttg gacgaaagcg cagcggagcc
     2581 agaacctgtc cgtcgaggag gacacctcct ggcacgtcac cggcagagcc agccaccgcc
     2641 tcagttccgc cagcccggcc ggggtgagcg agcagcgttt tctttgtctc gggttgtcgc
     2701 cggtttcgcc ggccgcgcga atccagcgcc gcttctccag ccgggccagc gccgggtaga
     2761 tggagcccgg gctgtcgctg aagctgccga tcggcgtagt ggcgaagacc ttgcgcaact
     2821 cgtagccgga ctgggccttc tgatggagca ggcccatgag agcataacca agaacggtgg
     2881 cttccgggac tttcatagta tgctacgtac tattataata cgttgcgtac tattgtcaag
     2941 aggccggcgg ctggctgtgc acaacgctcc caaacggtcg cg
//
LOCUS       DATCGS010000434         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_581668, whole genome shotgun sequence.
ACCESSION   DATCGS010000434 DATCGS010000000
VERSION     DATCGS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581668"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..837
                     /locus_tag="VIL39_08980"
     CDS             202..837
                     /locus_tag="VIL39_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEY5481597.1"
                     /translation="MKLFHTKIIVPICGAAMAVLVAGCASSGVKNPSGVSVTHMNADE
                     QGFVAGTGVESQDLVMVADKMARSILAIPQIANAATPPTIVLNPVENQTRFPINKDIF
                     LIRIRAQLNSKAMGKVMFLARNQMEALEKERNLKREGSVTASSDPGIQEFKGADYFLT
                     GTLQGMSTRTKAGTSDYILYDFHLIDARTSGIMWEDSAEIKKQGLEDAAYR"
     gene            856..2040
                     /locus_tag="VIL39_08985"
     CDS             856..2040
                     /locus_tag="VIL39_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481598.1"
                     /translation="MMKSLWQPTLLAALLSMAGCATTTKQPVALTGDIMADAPNAIAN
                     GPPRDRVLWQYRLAAAAMRQGKFDLAKQNLDDALLTLGGIYGKDKDAKKARSNFHAEA
                     KKTFIGEPYERAMAYIYRGILYWMDGEPDNARACFRSAEFEDSDAQSHEYAGDWVLPE
                     YLDGLATIKLQGDGSDAFKRAQANAKGVKLPPYLPKANALFFVEFGPGPAKFATGEYG
                     EELRFRSVPSQVSSADLKVDALQIPIAPTDDVEFQATTRGGRIMDHILGNKAVFKSTT
                     DIVGNVALIGGLTTAAVSRDRTTQEVGLGIALAGLVSKVISAVTVPAADTRAWDNLPR
                     YLSFVSLPLPPGPHVATIQFHDSTGQVLPNLTKTININITTTDRDKVVFVSDTSLTPQ
                     TL"
     gene            2037..2462
                     /locus_tag="VIL39_08990"
     CDS             2037..2462
                     /locus_tag="VIL39_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481599.1"
                     /translation="MNKSLLSLLTAGGALLLVAGCCSTHDKGPYLPQIPKTPAHETYA
                     GVVLLDPGVQYSVTCSGVQEQTLPDGRLEVIAHLRNRENRRIEVQANCVFKDLNAFST
                     GDETPFATVILTENGTEDVRFVSMNNLAKKCTIRVRQAR"
     gene            2790..3053
                     /locus_tag="VIL39_08995"
     CDS             2790..3053
                     /locus_tag="VIL39_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481600.1"
                     /translation="MAHSSPREEFKEKRWAELGALVRFARRDVPVELAQTDPALYRQL
                     REQITRFLLLDGDVFSLEALDAKSQSKSLSAEVKSCCSERGSS"
BASE COUNT          662 a          957 c          949 g          542 t
ORIGIN      
        1 ctactattac cctgcgccag tttatgtgca gccggtgtat caggcctatc cgtctgccca
       61 gccacccgta tctccggctg cgccggcgcc ccaagcgtat cggccggccg ccaatcgggt
      121 ctgggctcac cagatcccgg acgcgccgct cgtgccggac gcgccaacgt tttaacaggt
      181 taacttccaa ttcactaaat catgaaacta ttccatacca agatcattgt tcctatttgc
      241 ggcgcggcca tggccgtgct cgtggccggt tgtgcctcca gcggcgtgaa gaatccgtcc
      301 ggggtttccg tgacgcacat gaacgcggat gaacagggct ttgtggccgg caccggcgtc
      361 gagtcgcagg acctggtgat ggtcgccgac aaaatggcgc gcagcatcct ggccatcccg
      421 cagatcgcca acgcggcgac gccgccgacg atcgtgttga atccagtgga gaaccagacg
      481 cgctttccca tcaacaaaga catcttcctg atccgcatcc gggcccaact gaactcgaag
      541 gcaatgggca aggtgatgtt cctggcacgg aaccaaatgg aggcgctgga gaaggagcgg
      601 aacctgaagc gcgagggcag cgtcaccgcg agttccgatc ccggcatcca ggagttcaaa
      661 ggcgcggatt atttcctcac cggcacactg caagggatgt ccacccgtac caaggcgggc
      721 acgagcgatt acatcctgta tgatttccac ctcattgacg ctcggaccag cggcatcatg
      781 tgggaagatt cggcggagat caagaagcag ggcctggaag acgccgctta tcgttaagcc
      841 tcatcgcggg gcgacgtgat gaaatcactc tggcagccga cgctgctggc cgctttactg
      901 agcatggccg gctgcgccac gacaacgaag cagccggtcg cgttgactgg cgacatcatg
      961 gcggacgccc cgaacgccat tgcgaacggg ccgccgcgtg accgggtgct ctggcaatat
     1021 cggctggcgg cggcagcgat gcggcagggg aagttcgacc tggctaaaca gaacctcgac
     1081 gacgcgctgc tgaccctggg cggcatttac ggcaaggaca aggacgcgaa gaaagcccgg
     1141 agcaatttcc acgcggaggc gaagaagacg ttcatcggcg agccttacga gcgggcgatg
     1201 gcctatattt atcgcggcat actctattgg atggacggcg aacccgacaa cgcccgcgcc
     1261 tgcttccgca gcgccgagtt cgaggacagc gacgcgcaga gccacgaata cgcgggcgac
     1321 tgggtgttgc ccgaatacct cgatgggctg gccacgatca aacttcaggg cgacggctcg
     1381 gacgccttca agcgcgctca agccaatgcc aaaggtgtca aactaccgcc ttaccttccc
     1441 aaagcgaatg cgctgttctt cgtcgagttc ggcccgggcc cggccaaatt cgccaccggc
     1501 gagtacgggg aggaacttcg ctttcgcagc gtgccatccc aggtgtcatc ggcggacctc
     1561 aaggtagatg ccttgcagat ccccatcgcg ccgacggatg atgtggagtt tcaggcgacc
     1621 acgcgcggcg ggcgcataat ggaccatatc ctcggcaata aagcggtgtt caaatccacg
     1681 acggacatcg tgggcaacgt ggcgctcatc ggcgggctga cgactgcggc ggtgagccgc
     1741 gaccgcacga cgcaggaagt cggcctcggc atcgcgctgg ccgggttggt cagcaaagtg
     1801 atttcggccg tgacggtacc ggccgccgac acccgggcct gggacaacct gccgcgttac
     1861 ctgagcttcg tgagcctgcc gctgccgccc ggaccgcacg tcgccaccat ccaatttcac
     1921 gattccacag gtcaggttct tccgaacctg accaagacca tcaacatcaa catcacgacg
     1981 accgaccggg acaaggtcgt gttcgtcagt gatacctcat taacaccgca aacgctatga
     2041 acaaatcact cctatcactg ctcaccgccg gcggggctct actgctggtc gccggttgct
     2101 gcagcaccca cgacaaaggg ccttacctgc cgcaaatacc caagaccccg gcccacgaga
     2161 cctacgctgg ggttgtcttg ctggatcctg gcgtgcaata ctccgtcacc tgcagtggcg
     2221 tccaggagca aacgcttccg gatggccggc tggaggtgat cgcccacctg cgcaaccgcg
     2281 agaaccgccg cattgaggtc caggccaact gcgtcttcaa ggacctgaac gcattctcga
     2341 ccggcgacga gacgccgttc gcaacggtga tccttacgga gaacggcacc gaagatgtgc
     2401 gatttgtctc catgaacaac ctcgcgaaga agtgcaccat ccgcgtgcgg caagcgcgat
     2461 agcccgcaaa tttctcaggg aacgagggag cgcacggttc gataacgaac atgggcttgg
     2521 cacagggtat ggagttccgg ctttagccgg ctgggcgtta tcctttgaag gcggctcgaa
     2581 acgtttggaa atccatggca acagcgcgtc tgaccggcta aagccgggac tccataccct
     2641 gtggccttcg ttcgcgtgtg aaatatgcgc gctgagcggg tggtgcgcag tgacggggtg
     2701 gaaacggcgg gcagcggggc gcagagcatt agtctgcctc acgccgcagg acgaacagtt
     2761 accgccccgc agtcaggccg ggagccggat tggcccatag tagcccgcgt gaagaattca
     2821 aggaaaagcg ctgggccgaa ctgggggcat tggtgcgctt tgcccggcgg gacgtgccgg
     2881 tggaactggc ccagaccgac ccggccctct accgccaact gcgggaacag atcacccggt
     2941 tcttgcttct ggatggcgat gtcttctcgc tcgaagcctt ggatgccaag tcacaaagca
     3001 aatccctgag cgccgaggtg aagtcatgct gctccgagag gggaagttcc taaagttcgg
     3061 ggctaaaagc cccaaagttc agactgaaag aactagaggg gctcaagtaa
//
LOCUS       DATCGS010000435         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_186978, whole genome shotgun sequence.
ACCESSION   DATCGS010000435 DATCGS010000000
VERSION     DATCGS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186978"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..2147
                     /locus_tag="VIL39_09000"
     CDS             111..2147
                     /locus_tag="VIL39_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481601.1"
                     /translation="MKRISLLLIVTVTAAILAGQNKTPESTLGAALHQEEVQGDLKGA
                     IAAYNKLLAGRGLSHKVAAEALYHLGLCYQKLGDADARKAFERLLAEYPDQTGWVAQA
                     RARLAALGAPAQPAVVRAWTDLGSKGVRPIDFAISPDGTFVAYGRRAQGGDYTPSDDI
                     WVHPLAGGEDRAITKHTGGTAALPVVSPDGRFVAYSRAIRSTMELRVANVDGSGDRAL
                     VSRTLDEQLVPYAWSPGGANILVGRNDSRELGMMQVVGGGITVLQSGQNLAAKPNAFS
                     PDGKYVVYTSGGMTNSRIWIMSTGGGGEIEIASDPAGAWHNGQAGWTPDGRLLFWSNR
                     SGSQALYWQRMAEGKPQGEPVRIDDVRLAPIDEIGRAGVQVARDGSLFYAARTRQTDV
                     YTAHFDPASMQLTSAPQRLNQRYVGDVRTLSDWSPDGGSILYTSVQPAGGVVVHSFGG
                     NEEREIKPSGWVGQMRFFPDGQSVMSNKDGRMDLRTGIVSALGAKMPNTFEWDLSPDM
                     QFLYYGAVVEPVPTSGPRKLRFIRRSIVSGEETDVLSLETSRNQPFNISVSPDGKQVS
                     FNYWDAQNPSHMWLAAAHTEGGPVKQLVVEKNAGNKCVWTKDGKHLLCPRANEILVIP
                     ADGGASHPLSVGMNGMGLSDLALNPANNLIAFTATQTRIDLMSYKNVFPPASPR"
     gene            complement(2183..>2671)
                     /locus_tag="VIL39_09005"
     CDS             complement(2183..>2671)
                     /locus_tag="VIL39_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481602.1"
                     /translation="VLGHNLRELSERKIAHWRNENVGFIFQQFNLIPVLTALENVELP
                     LKLTNLKKAERIAHAITALTLVGLGDRLSHFPRQLSGGQEQRVAIARAIVTDPALILA
                     DEPTGNLDAASALEVLTVLGKLNKEFGKTVVMVTHDPHAAQFATVVHHLEKGELLAAR
                     VN"
BASE COUNT          527 a          791 c          845 g          508 t
ORIGIN      
        1 cgccacatcg tcagtatgcg cggcttggga taccgcttcg atgtctgatc ttgaaacaat
       61 cttgaccgga gcttgaatca tgaaaacgcg gaacgccgta atctgaggcc atgaaacgaa
      121 tttccctgct tctcatagtg acggtcacgg cggcgattct cgccggccaa aacaaaacgc
      181 ccgaatcgac actcggcgcg gccctgcatc aggaagaggt gcaaggcgat ctgaaaggcg
      241 ccatcgcggc atataacaag ctgctcgccg gccgcggcct aagccataag gtggccgcgg
      301 aagcgctgta ccatctcggg ctgtgttatc aaaagctggg cgacgcggac gcccgcaaag
      361 ccttcgagcg gctgttggcg gaatatcccg atcaaaccgg ctgggtagcg caggcgcgcg
      421 cgcggctggc ggcgctgggg gccccggcgc aacccgctgt cgtccgggcg tggactgatc
      481 tgggatctaa gggagtacgg ccaatcgatt ttgccatatc tccggatggg accttcgtgg
      541 cgtacgggcg acgcgcccaa ggcggcgact acacgccaag cgacgacatc tgggtacacc
      601 ccctggcggg cggagaagac cgcgcgatca cgaaacacac gggcggcaca gcggcgctgc
      661 ccgtggtttc gccggacggc agatttgtcg cgtactcgcg ggcgatcagg tccacgatgg
      721 agctgcgcgt ggctaacgtg gacggttcgg gcgaccgcgc gttggtgagc cgtacacttg
      781 acgaacagct cgtcccatat gcctggtcgc cgggcggcgc gaacatactg gtgggccgga
      841 atgactcaag ggaactcggc atgatgcagg ttgtgggtgg cggcataacc gtcctgcagt
      901 ccggacagaa cctggcggca aaacccaatg cgttttcgcc cgacgggaaa tatgtggtct
      961 acaccagtgg tggtatgacc aacagccgga tttggataat gagcacgggc ggaggtggag
     1021 agatcgagat cgcctcggat ccggccggcg cctggcacaa cggccaggca ggatggacgc
     1081 ccgacggacg gcttctcttc tggagcaacc gctccggaag ccaagcgctg tattggcagc
     1141 gtatggccga gggtaagccc cagggcgagc cggtacggat cgacgatgta cgtttggcgc
     1201 ctatagacga gattggtcgc gcgggagtgc aggttgcgcg tgatggttcg ctcttctacg
     1261 ccgcacgaac tcgtcagacc gacgtctata ctgcccactt tgatcccgcg agtatgcagc
     1321 tgacttcggc cccccaacgg ttgaaccaga ggtatgtcgg cgacgtgcgt accttgtccg
     1381 actggtcccc ggacggcggt tcaatcttgt acacgtctgt ccagcctgca ggaggcgtag
     1441 tggttcactc cttcggcggc aatgaagagc gcgagatcaa gccttcgggc tgggtgggac
     1501 aaatgcgctt cttcccggac ggccagtccg tcatgagcaa caaggatggc cgtatggacc
     1561 tgcgaaccgg cattgtcagc gcacttggcg cgaagatgcc caacacgttt gaatgggatt
     1621 tgtcgccgga catgcagttt ctctattacg gcgctgtggt cgagcccgtt cccaccagtg
     1681 gcccgaggaa gcttcgtttc atccgtcgca gtattgtcag cggcgaagaa accgacgtcc
     1741 tgagccttga aaccagtaga aatcaaccat ttaacatttc agtttcaccg gatggcaagc
     1801 aggtttcgtt caattactgg gatgcgcaga acccgagcca tatgtggctg gctgccgcgc
     1861 acaccgaagg cggccccgtg aagcaactgg tggtggaaaa aaacgcgggc aacaaatgcg
     1921 tatggacgaa ggacggcaaa caccttttgt gcccgcgagc caatgagatc ctcgtgattc
     1981 ccgccgacgg cggcgcctcg caccctctca gcgtcgggat gaatggcatg ggactgtccg
     2041 atctggcgtt gaatcccgca aacaacctca tagccttcac cgcgacgcag actcggattg
     2101 acctgatgtc ctacaagaac gtgtttccgc cggcttcgcc gcggtagctg ccatttggtc
     2161 gggggcgtga ttcgcttctc gcttaattga ctcttgcagc cagcagctcg cccttctcca
     2221 ggtggtgcac tacggtggcg aactgggcgg cgtgtgggtc atgagtcacc atcaccacgg
     2281 tcttgccgaa ttccttgttc agcttcccca ggaccgtcag gacttcgagc gccgaggccg
     2341 cgtccaaatt gccggtaggc tcgtcggcca ggatcagggc ggggtcggtc acgatcgcgc
     2401 gcgcgatggc tacgcgctgc tcctggccgc cggaaagctg ccgcggaaag tgcgacaggc
     2461 ggtcgcccag gccgaccagc gtcagcgccg taatcgcgtg cgcgatcctc tcggccttct
     2521 tcaggttcgt gagcttcaac ggcagttcga cgttctccag cgcggtcagc accggaatca
     2581 ggttgaattg ctggaagatg aaccccacgt tctcgttgcg ccagtgcgcg attttccgct
     2641 cgctcaactc gcgcaggtta tggccaagca c
//
LOCUS       DATCGS010000436         5036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_187043, whole genome shotgun sequence.
ACCESSION   DATCGS010000436 DATCGS010000000
VERSION     DATCGS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187043"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1159
                     /locus_tag="VIL39_09010"
     CDS             296..1159
                     /locus_tag="VIL39_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5481603.1"
                     /translation="MISPFARSHAAESQRPAAQKTLVGSNSYGWGQYAQRDKKKFDVE
                     EVISALRDAGYDYLEHTMNVDQPEENAKFAAQLKAKGLQPVSLYTGARVHEAAQAGAV
                     VARILAAAKVCQQAGFRVISCNADPIGREKTDEELKTQAAALSELGEGLNALGLKLGM
                     HHHMPEMANHAREFHYNFEHTKLAVVGFCYDVHWVWKGGVMPLDALKQYGDRVVTWHL
                     RQSRDGVWWEDLDTGDVDYEVVAQYAKANGLPRRFTVELAIEGATKITRSVVENHRRS
                     REFVRRVFESQ"
     gene            complement(1288..2535)
                     /locus_tag="VIL39_09015"
     CDS             complement(1288..2535)
                     /locus_tag="VIL39_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5481604.1"
                     /translation="MNPSGTKEPAALPPAREPLASTSEAAVAAGAKLRHAAILAAVLI
                     VIGAVAGLVPRWLHRTALRAETRELAIQTVSVVSPVPGKAAVGLTLPAEAKALVEAPI
                     YARTSGYLKRYLVDIGAQVKAGDLLAEIDTPELNQELAQARAQLTQTEAALALARMTA
                     ARWADLVKTASVSEQEAAEKQADLELKAATVEAARANVRRLENLQSFERVTAPFAGTI
                     TARGTDVGQLVASISGKELFRLAQTGTLRVYVRVPQTTALGVAPGQMAELTIPELPGR
                     VFPAKVVRTSGAMSADSRTLLTELEVDNSRGEILAGTYVQVRLTEAKLDPVLTLPSNT
                     LLFRAEGTQVGVVGVGDKVELRRVTLGRDFGATIEVLGGVGLTDRVILNPADSLVGGT
                     TVRVAEAAKEPAGKAASTAASAK"
     gene            complement(2544..>5036)
                     /locus_tag="VIL39_09020"
     CDS             complement(2544..>5036)
                     /locus_tag="VIL39_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5481605.1"
                     /translation="PIRTVNGALIRVRDVAQVRDGYQPQQNIVRNDGVRGALLTIYKT
                     GSASTLDVVSGVKRALPRVLRGLTADLDVKEFADQSIFVRAAISGVVREGVIAAALTA
                     MMILLFLGSWRSTLIIALSIPLSVLTSIAILSALGETINLMTLGGLALAVGILVDDAT
                     VAIENIHRHMASGKALDDAILDGAQEIAMPAFVSTLCICIVFVPMFFLTGVAHYLFVP
                     LAEAVVFAMLASYVLSRTLVPTLVMWFYRNVEHSSGHEEPAATALWLRPFAAIQTRFE
                     RGFDRFRDGYRILLGAVLHHRTPFAAIFLLFCIGSMLFVPQLGQDFFPSVDAGQFRLH
                     LRARSGTRIEETAKLVDEVEATIRQEIPASEFRGMLDNIGIPGSSINLTYNDSGLIGT
                     GDADILVALRPEHGPTAGYVDRLRARLNREFPGITFYFLPADIVSQSINFGLPAPFDI
                     QLVGRNQVKSREVAGRLADQIRRVSGAVDVRVQQPADLPKLSVEVDRTKASGMGLAER
                     DVGNSVLLSLSGSGQVSPVYWIDPRIGIQYLVNVRVPERQMDSLAALGSIPINASQPG
                     EGNGQLLANLATVTRSAGPPVISHYNVMPVIDIFGGVSGRDLGGVLHDLKPLIAQAEK
                     ELPKGSSIILRGQAETMHSSFVGLGVGLVMAIVLIYLLLVVNFQSWLDPFIIITALPG
                     ALAGVVWALHLTLTTASVPALMGAIMSLGVATANSVLVVTFARNDLQQGMDPLTAAWE
                     AGVSRLRPVLMTAAAMVIGMFPMALGLGEGGEQNAPLGRAVIGGLVVATIATLFFVPV
                     VFSLLQRRATVPAKTPSDFSPASGITPASAGA"
BASE COUNT          948 a         1574 c         1677 g          837 t
ORIGIN      
        1 acgaggttct tcgcgcagcc aagcgaaatc atgcccacgc gcatcgggcg ctttatattc
       61 tgttcgttca tcaaagaccc gacaccttac gtcagggaga tagtgcgggc aacctttcgg
      121 gtttggcctc ttgctccggc ccccgcgtcg aggcgagttt tctctggcgc agaaggcgga
      181 gcgagctttc aataggtcta tgttgaaatg ctttccattc gttcacgcat cagttgttcg
      241 ctcatcaagg cgtgagttta tccgtcacgt cggatcagcg gtggcactgg gcgcagtgat
      301 atctcccttc gcccggagcc acgcggcaga gagtcagcgc cccgcggcgc agaagaccct
      361 ggtcggctcc aatagctatg gctggggcca atacgcccag cgcgacaaga agaagttcga
      421 cgtggaggaa gtcatctccg cgctgcgtga tgccggctac gattacctgg agcacaccat
      481 gaacgtggat cagccggagg agaacgcgaa gtttgccgcg cagctcaaag ccaagggcct
      541 ccagcccgtc agcctttaca ccggcgcgcg cgtgcatgag gccgcccagg ccggcgcggt
      601 ggtggccagg attctcgccg cggccaaagt ctgccagcag gccggcttcc gcgtcatcag
      661 ttgcaacgcc gacccgatcg ggcgcgagaa gaccgatgag gagttaaaga cgcaagcggc
      721 tgcgctcagc gaattgggcg agggcttgaa tgcgctcggc ctgaagctgg gcatgcacca
      781 tcacatgccg gagatggcca accatgcgcg ggagttccac tacaatttcg agcacaccaa
      841 gctcgccgtg gtcggcttct gctacgatgt tcactgggtc tggaaagggg gcgttatgcc
      901 gctggatgcg cttaagcagt atggcgaccg cgtggtcacc tggcacttgc gccaaagccg
      961 cgacggcgtt tggtgggaag acctcgacac cggggacgta gattacgaag tcgtggcgca
     1021 atatgccaag gcgaacggcc tgccgcggcg attcacggtc gaactggcca tcgaaggcgc
     1081 caccaagatc acccgttctg tggtggagaa ccatcgccgg agccgggagt tcgtcagacg
     1141 agtgtttgag agccagtgag ggggaagggg cccaaattgg gtagcctgac tacccaaagc
     1201 caattgactc tgctagcaac cccttgaaaa acctggcgat gcgtttcagt ctttcagcgt
     1261 ttcagctttt gagggccatc cgttcgccta ttttgcgctt gcggccgtgg atgccgcctt
     1321 gcccgcaggc tccttcgccg cttcggccac gcgcaccgtg gtgccgccca cgagcgaatc
     1381 ggccgggttg aggatgaccc ggtcggttaa gccaactccg cccaggacct caatggtagc
     1441 cccgaagtcg cggcccagcg tgacgcggcg caattcgact ttatcaccaa cgcccacgac
     1501 acccacttgg gtcccctcgg cacggaatag cagggtattc gagggcagag tcaggactgg
     1561 gtccagtttg gcttccgtta agcggacctg gacgtaagtg ccggcgagga tttcgccgcg
     1621 cgaattgtcc acctccaact cggtgaggag ggtgcgcgag tcggcgctca tggccccgga
     1681 ggtccgcacc actttggccg ggaacacgcg cccgggcagt tcggggatgg tcagctccgc
     1741 catctggccc ggagcgacgc ccagggcggt ggtctgcggc acgcgcacat acacgcgcaa
     1801 ggtaccggtc tgggccaggc ggaagagttc cttgccgcta atcgaggcga ccagttggcc
     1861 gacatcggtg ccccgcgcgg tgatggtgcc ggcgaagggg gcggtgaccc gctcgaagga
     1921 ttggaggttt tcaagccggc gcacattggc gcgcgccgcc tcgacggtcg cggccttgag
     1981 ttccaggtcg gcctgtttct cggcggcttc ctgctcgctg acactggcag tcttgaccaa
     2041 gtcagcccag cgggcagccg tcatcctggc cagcgcgagg gcggcctcgg tctgggtgag
     2101 ctgggcgcgg gcctgggcca gttcctgatt cagctccggg gtgtcaatct cggcgagcaa
     2161 atcccccgcc ttgacctggg cgccgatatc caccaggtag cgcttaaggt agccgctggt
     2221 gcgggcgtag atcggcgctt cgaccagggc cttggcttcg gcgggcagcg tcagtccgac
     2281 ggcggccttc cccggcaccg gcgacaccac gctgacggtt tggatggcca gctccctcgt
     2341 ctcggcgcgc aaggcagtgc gatgcagcca gcggggcacc agcccggcca cggcaccaat
     2401 gacaatgagc acggcggcca gaatggcggc gtggcgcagt ttcgcaccgg cggccacggc
     2461 ggcttccgaa gtcgaggcca acggttcccg ggccggcggc agcgcggccg gttctttggt
     2521 tccagaagga ttcatagtta aactcaggcg ccggcagagg cgggggttat gccgctggcc
     2581 ggcgagaaat cggagggcgt tttagcgggc acagtagcgc gccgttgcaa caggctgaag
     2641 acgacgggca cgaagaacag cgtggcaatg gtggccacca ccaggccgcc gatcaccgcg
     2701 cggcccagcg gtgcgttctg ctcgccgcct tcgcccaacc cgagcgccat cgggaacatg
     2761 ccgatgacca tagcggcggc cgtcatcagc accgggcgca agcggctgac tccagcttcc
     2821 caagccgccg tcagcgggtc catgccttgc tggaggtcgt ttcgcgcgaa cgtgaccacc
     2881 agcacggaat tggcggtcgc gacccccagg ctcataatcg cccccatcag cgcgggcacg
     2941 ctggcggtgg taagcgtgag atgcagcgcc cagaccacgc cggccagcgc gccgggcagc
     3001 gcggtgatga tgatgaacgg atcaagccag ctttggaagt tgaccaccag cagcaggtag
     3061 atcagcacga tcgccattac caatccgacg cccagcccga cgaaactgga atgcatggtc
     3121 tcggcctggc cgcgcaagat aatcgagctg cccttgggaa gctccttttc cgcttgcgcg
     3181 atcaggggct tgaggtcgtg cagcacgccg ccgaggtcgc gcccgctgac gccgccaaaa
     3241 atgtcaatca ccggcatgac gttgtagtgg gaaatcacgg gagggccggc gctgcgagtg
     3301 accgtggcga gattggccag cagttgtccg ttcccttcac cgggctggct ggcgttgatc
     3361 gggatcgagc caagcgcggc cagggaatcc atctggcgct cggggacacg aacgttgacc
     3421 aggtattgaa tcccgatgcg cggatcaatc caatagacgg gcgacacctg gccgctgccg
     3481 ctcaggctga gcaagaccga attgcccaca tcgcgctcgg ccaggcccat gccggaggcc
     3541 ttggtgcgat caacctccac gctgagtttg ggcaggtcgg ccggctgctg cacgcgcacg
     3601 tccaccgcac ccgacacccg gcgaatctga tcggcgagcc gcccggcgac ctcgcggctc
     3661 ttcacctggt tgcggccgac cagttgaatg tcgaacgggg ccggcaggcc gaagttgatg
     3721 gactggctga cgatgtccgc aggcaggaaa tagaaggtga tgcccggaaa ctcccggttg
     3781 agccgggcgc gcaaccggtc cacgtaaccg gcggtcggac cgtgctcggg gcggagcgcc
     3841 accagaatat ccgcatcgcc ggtgccaatc aggccgctgt cgttgtaggt caggttgatg
     3901 ctggagccgg gaatccctat gttatccagc atgccgcgga attccgaggc ggggatttcc
     3961 tggcgaatgg tggcctcgac ctcatccacc agtttggcgg tctcctcgat gcgggtcccg
     4021 ctgcgggcgc gcaggtgcag ccggaattgc cccgcatcca ccgagggaaa gaaatcctgc
     4081 ccaagctgcg gcacgaatag catcgagcca atacagaaca ggaggaaaat cgccgcaaag
     4141 ggggtgcgat ggtgcagcac ggccccaagc aggatgcggt agccgtcgcg gaaacggtcg
     4201 aagcctcgct cgaaccgggt ctggatggct gcgaatggcc gcagccaaag agcagttgcg
     4261 gccggctctt cgtggcccga ggagtgttcc acattgcggt aaaaccacat caccagcgta
     4321 ggcaccagcg tccgggagag cacatagctg gccagcatcg cgaacaccac ggcctcggcc
     4381 agcggcacaa acaggtagtg cgccacgccg gtcaggaaga acatcggcac gaacacgatg
     4441 cagatgcaaa gcgtggagac gaaagccggc atcgcgatct cctgcgcgcc gtccaggatg
     4501 gcgtcgtcca gcgctttgcc cgacgccatg tgccggtgga tgttctcgat ggccacggtg
     4561 gcgtcatcca ccagaatgcc gacggccagc gccaggccgc cgagggtcat caggttgatg
     4621 gtttcgccca gcgcgctgag gatggcgatg gaagtgagca ccgagagggg gatggagagc
     4681 gcaatgatga gcgtgctccg ccacgagccg aggaagagca gaatcatcat cgccgtaagc
     4741 gcggcggcga tgacgccttc ccgaaccacg cctgagatgg ccgcgcggac gaagatagat
     4801 tggtcggcaa attccttcac gtccaggtca gcggtgaggc cgcgcaagac gcgcggcagg
     4861 gcccgcttca cgccgctgac cacatcgagg gtggacgcgc tgccggtttt atagatggta
     4921 agcagcgcgc cccgcacgcc gtcgttgcgc acgatgttct gctgcggctg gtaaccgtcc
     4981 cgcacttgcg ccacgtcccg cacgcggatg agcgcgccgt tgaccgtccg gatggg
//
LOCUS       DATCGS010000437         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_87010, whole genome shotgun sequence.
ACCESSION   DATCGS010000437 DATCGS010000000
VERSION     DATCGS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87010"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VIL39_09025"
     CDS             <1..253
                     /locus_tag="VIL39_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481606.1"
                     /translation="LDEDERSINDGFFVTDPTGKIWEDFPAMSAQRHSYSYALSFADG
                     HSEIWRYRDSRTYQVSLHKTEQPGNADLERLARATVTAK"
     gene            526..1617
                     /locus_tag="VIL39_09030"
     CDS             526..1617
                     /locus_tag="VIL39_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481607.1"
                     /translation="MNKSAKIEETREPLSFATVLRNQLSCGVILIDGGKKVLVLTGQA
                     RHLLGLDPDQAILPAFETLPAPVQAIVCETLASGKALNDRQIEMDAGSRGEITLHFSA
                     VPLEPNRKNSGVMVALNDLTAARQLEERLERLDRLANMGILAATMAHEIKNALVPGKT
                     FIDLLLEKNKDAELVEVVRREIGRIDAIVSRMLRFAGPAKADFSEVRLHEVLEHSLRL
                     VQPQLEDKSTVLSRSFQAAPDRLKGDDYQLQQAFVNLFLNAVESLGSNGTLSVTTETC
                     SPDAKLARPGDLMTQPHLRIVIKDTGIGIAPENMARLFEPFFTTKPKGTGLGLAITRR
                     IIMEHRGAISVESQPGQGTTFQILLPTSA"
     gene            1676..2653
                     /locus_tag="VIL39_09035"
     CDS             1676..2653
                     /locus_tag="VIL39_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481608.1"
                     /translation="MGTCKLGILSDIHYASAAEQARGDDYEIRGITNPLARAFARSFR
                     HFVWLREPLRKNHLLDQFVEGGGSFDYVIANGDYSCNSACVGLSDDAAYQSARECLQK
                     LRQRFGVNLRANYGDHELGKISTFGHRGGMRLASWHRTREELGLPPFWQLEIGHYTLM
                     GFVSSLVALPVFEAEMLPAERPEWERLRAEHLAEIRRAFAALRPSQRVLLFCHDPSAL
                     PFLWREETVRARLPQIEHAIIGHLHSNLVLWSSRRLAGMPPIRFLGHTVQRLSTALNQ
                     ARYWRPFNVRFCPALAGTELLKDGGYCTAELDPEARHPVRFDFHPLPGP"
BASE COUNT          544 a          935 c          760 g          516 t
ORIGIN      
        1 cctggatgaa gacgaacgca gcatcaacga cggtttcttt gtcaccgacc ccaccggcaa
       61 gatttgggag gatttccccg ccatgtccgc tcagcgccat agctatagct acgccctcag
      121 cttcgctgac ggtcactccg aaatatggcg gtacagggac tcgcgcacct atcaggtcag
      181 cctgcacaaa accgagcaac ccggcaacgc ggacctcgaa cgcctggccc gcgcgaccgt
      241 caccgcgaaa tagccacccc gcggatccca ctacattacc gcctcggaga cgttccggca
      301 gaagaagact tggcggctct ttgaaggagg tggcgcatag ttatgcgggc cgctctatcg
      361 gcctgaactt ctcccgacag aatatcgttt tcttgttgtt gccccggcaa agggaggtta
      421 acattcctgt tccatggtaa ggcatcgctt ttgtgactgt cgcccgcgtc tgggcctcct
      481 cgtgcgcggc gtcagcacca acctcgtcgg gcggcggcta tcttaatgaa caaatcggcc
      541 aaaattgaag agacccggga gccgttaagt ttcgcaaccg tgttgcgcaa ccagctctcc
      601 tgcggggtca tcctcattga cggcggaaag aaagtcctcg tcctcaccgg ccaggccaga
      661 caccttctgg gcctggaccc ggatcaggcc atcctgcccg cttttgagac cctgccggcg
      721 ccggtccaag caatcgtgtg cgagaccttg gcctcaggca aggcgctcaa cgaccgccaa
      781 attgagatgg acgccggcag tcgaggcgag atcacacttc acttcagcgc cgttccactg
      841 gagccgaacc gaaagaattc gggcgtcatg gtcgcgctca acgacctgac cgcggcccgg
      901 caactcgaag aacgccttga gcgactcgac cggctggcca atatgggcat tctcgccgct
      961 accatggccc acgaaatcaa gaatgccctt gtccccggga agaccttcat agatctgctg
     1021 ctcgagaaaa acaaggatgc cgaactggtt gaagttgtcc gccgcgaaat tggccgcatc
     1081 gatgccattg tcagccggat gctcaggttc gccggaccgg ccaaggcgga cttcagcgaa
     1141 gtccgcctcc acgaagtcct ggagcactcc ctgcgcttgg tgcagcctca actggaagac
     1201 aaatctactg tcttaagccg gtccttccag gccgccccgg accgcctcaa gggcgatgat
     1261 taccagttgc agcaggcttt cgtgaatctc ttccttaacg cggtcgaatc gctggggtca
     1321 aacggcactc tatccgtcac cacggaaacc tgttcgccgg atgccaagct cgcccggcca
     1381 ggagatttga tgacccagcc acacctgcgg attgtgatca aagacacagg catcggtatt
     1441 gcgccggaga atatggcgcg cctgttcgag ccgtttttca ccaccaaacc caagggcacc
     1501 ggtctgggcc tggccatcac gcggcgcatc atcatggaac accggggtgc cattagcgtg
     1561 gagagtcagc ccggccaagg caccaccttc caaatcctgc tcccgacttc cgcttgattg
     1621 aaagtggcac aggccgggtt gacgacggcg cgattgcggc tccgacatga gcgccatggg
     1681 cacctgcaaa ctcggcatac tgagcgacat tcattacgcc agcgccgccg aacaggcgcg
     1741 cggagacgat tatgaaattc gcggcatcac gaatcccctg gcgcgtgcct tcgctcgttc
     1801 cttccgccac ttcgtctggc tgcgggagcc gctgcgcaaa aaccacctcc tcgatcagtt
     1861 cgtcgaaggc gggggctcgt tcgattacgt gattgccaac ggcgactact cctgcaactc
     1921 ggcctgcgtc ggcctgagcg acgacgcggc ctaccagagc gcgcgggaat gcctgcaaaa
     1981 actgcgacag cgattcggcg tcaacctccg cgccaattat ggcgaccacg agctaggcaa
     2041 aatctccacg ttcggccacc ggggcggcat gcgcctggcc agttggcatc gcacccggga
     2101 agaactgggc ttgcccccat tctggcaact cgaaatcggc cactacaccc tgatggggtt
     2161 cgtctcatcc ctcgtcgccc tgccggtgtt cgaagcagaa atgctgcccg ccgagcggcc
     2221 cgaatgggag cgactgcgag ccgaacatct agcggagatt cgccgcgcct ttgccgcttt
     2281 gcggcccagc cagcgcgtct tgctcttctg ccatgacccg agcgccctgc ctttcctctg
     2341 gcgcgaggag accgtccgcg cccgcctgcc gcaaatcgag cacgcgatta tcggccacct
     2401 gcattcaaac cttgtcttgt ggagcagccg ccggctggcc ggcatgccgc ccattcgctt
     2461 cctcggccac accgtccagc gcttgagcac cgccttgaac caggctcgct attggcgccc
     2521 gttcaacgtc cgattctgtc cggcgctggc cggcaccgaa ctcttgaagg acggcggcta
     2581 ctgcaccgcc gaacttgacc ccgaggcgcg ccaccccgtc cgcttcgact tccaccccct
     2641 gcctggccca taagcccgag gtggcaggct taccccacat tgttccctgc ctataccctg
     2701 gcttcggttc ccaatcacgc atcacgcatc acgcatcacc actggctttg aggag
//
LOCUS       DATCGS010000438         4869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_730587, whole genome shotgun sequence.
ACCESSION   DATCGS010000438 DATCGS010000000
VERSION     DATCGS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730587"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VIL39_09040"
     CDS             complement(<1..1068)
                     /locus_tag="VIL39_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481609.1"
                     /translation="MSETSNRRSDEFLKISDQFQLGVLTTESSHPVTANLSETAKRDV
                     SAALGLLFEADGDNVRKYREFAESGRAASIKDAVLRALKNGGKVFFTGCGSTGRLSIQ
                     LVSIWRDYWQRQRARGLACSPTPEEFENRAFSVMAGGDFALIKSVEGFEDFTEFGKKQ
                     IGDLGVSAKDVVFAITEGGETSFVIGTAWKGLEVGAKVYFVYNNPDDILCKHVKRSRE
                     VIEEPRIEKINLTTGPMSITGSTRMQATTIQLCVMLTVLEMVLRDLMKELEPKGPCAL
                     NSSSVPSEFLKKLVEMHGNLMSPELRSQLAKAVSLEETVYRASHKNSYFADRFGIDVL
                     TDTTERSPTYCSPPFRKFDDTS"
     gene            complement(1133..1741)
                     /gene="thpR"
                     /locus_tag="VIL39_09045"
     CDS             complement(1133..1741)
                     /gene="thpR"
                     /locus_tag="VIL39_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY5481610.1"
                     /translation="MANSEQFRLFIAVTIPEEVKTKIEEAQGKMRRALPERAVRWTRR
                     EQFHLTLKFLGDVDAARVEALGEAIRAACQGFGALHLRAERVGFFPDLRYPRVVWVGV
                     KDQAEQLPRLQGAVESATREYATEDKEERFTGHVTLARFKGIRRPEAEALDKAAAGMA
                     EQLFGQWTAYQVELMRSELLPQGARHSSVASIALADLPADLA"
     gene            complement(1754..2710)
                     /locus_tag="VIL39_09050"
     CDS             complement(1754..2710)
                     /locus_tag="VIL39_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5481611.1"
                     /translation="MTEIMQTIPLGVSTLTSSRLAYGCWRVAGTWAPAEVTAASRAAG
                     RRAIIAAYEAGYTLFDNADIYCQGEAERILGDVLKEVSGMRDRVVITTKGGIRPGGDP
                     QPDSPGRYDFSASYIIAACEQSLRRLGVEIIDLYLLHRPDYLADPEEVARAFSQLKAA
                     GKVRHFGVSNFRPTLVTALQAACPMPLVAHQVEISLAKLDAFTDGTLDQCLIERITPM
                     AWSPLAAGLIGGGATRLLPSQKTYHPEQFLPVVDAIAGVHGASRTVVALAWLLKHPAR
                     ILPIVGTTNPDRIRETTKAASLELTREEWYRLLIAARGEPVP"
     gene            complement(2901..3533)
                     /locus_tag="VIL39_09055"
     CDS             complement(2901..3533)
                     /locus_tag="VIL39_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481612.1"
                     /translation="MSLINDALRRAKEAQQQTPLLPSHDLPFRPVEPAQQRARRGLGL
                     LLPFALVAVALLTLLLAWELTRTRGAASVIEVNARTTPVPQAITPASAPAAQPAPVEE
                     PASPKELVTDTAAALPANATSTPAADPLANVQESEATNAPVITPPPPPQPPPLRLQAI
                     IFNPKRPSVMISGKTLFIGDKLGDMRVVAIKKDSAILAGAGQTNVLSVAQ"
     gene            complement(3530..4345)
                     /locus_tag="VIL39_09060"
     CDS             complement(3530..4345)
                     /locus_tag="VIL39_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5481613.1"
                     /translation="MYLEYYGLTEPPFDITPNPRFLFYSPKHREAFNHLLYGIRERKG
                     FVQMTGEVGAGKTTLCRAVLEQLDDRYSTALILNPIMSANELVKAIALEFGLKVSGLD
                     RLDMLAVINEFLLKQVERQKDSVLIIDEAQDLTDELLEQVRLLSNLETDNRKLLQIIL
                     FGQPELRGRLNNPRLRQLRQRITVRYHLPPLQRQEVIQYVQHRLHVSGGNGTPYFTGP
                     ALWRVHHYSKGIPRLVNAVCDKALLAGYVLQSKRISFRMVGQAVRELEGNINA"
BASE COUNT          875 a         1480 c         1546 g          968 t
ORIGIN      
        1 gctcgtgtcg tcgaatttcc ggaacggcgg cgagcagtag gtcggcgagc gctcggttgt
       61 gtccgtcagc acgtcaatcc caaaccggtc ggcgaagtag ctattcttgt ggctggccct
      121 atagaccgtc tcttcgagcg agacggcttt ggcgagttgg gaccgaagct ccggcgacat
      181 caggttgccg tgcatctcca ccagcttctt cagaaactcg gacgggacgg aggacgagtt
      241 cagcgcgcag gggcctttcg gctccagttc cttcatcaaa tcccgcaaca ccatttccag
      301 cacggtcagc atgacgcaaa gctggatggt cgtcgcctgc atgcgcgtcg agccggtgat
      361 tgacatgggg ccggtcgtga ggttgatctt ctcgatgcga ggctcctcga tgacttcgcg
      421 gctgcgcttc acgtgcttgc acagaatgtc atcggggttg ttatagacga aatagacctt
      481 ggcgccgacc tccagcccct tccaggccgt gccaatcacg aacgaggtct cgccgccctc
      541 ggtgatggca aacaccacat ccttggctga cacccccaga tccccaatct gcttcttgcc
      601 aaattcggtg aagtcctcga aaccttccac cgacttgatg agggcgaagt cgccgccggc
      661 catcacgctg aaggcgcggt tctcgaactc ttcgggcgtc ggcgagcagg ccagtcctcg
      721 tgcgcgctgc cgctgccagt aatcgcgcca gatggacacc agttggatgc tcaggcgccc
      781 agtggaaccg cacccggtaa agaacacctt ccccccgttc ttcagggcgc gcagcacggc
      841 gtccttgatg ctcgcagccc ggcccgactc ggcgaattcc cgatacttgc ggacattgtc
      901 cccatccgct tcgaagagga ggcccagtgc cgcgctcacg tcgcgcttgg cggtttcgct
      961 taggttcgcg gtgacggggt gggaagactc ggtggtcagg acgccaagct ggaactgatc
     1021 gctgatcttg aggaattcgt cggatcgccg attagatgtt tcgctcatgg ggattcggta
     1081 gttacttaat ctgttatcag tggggtgcag cttgccggtt gcgcggagtg ggtcaagcca
     1141 aatcggcggg taaatcggcc agcgcgatcg aggcaacgga gctgtgcctc gcgccctgcg
     1201 gcaggagttc actgcgcatg agttcaacct ggtaagccgt ccactggccg aagagctgtt
     1261 ccgccatgcc cgccgccgcc ttgtccaaag cttccgcctc cggtcgcctg atgcctttga
     1321 agcgggcgag cgttacgtgg ccggtgaatc gttcttcctt atcttcggtg gcatattccc
     1381 gcgtggcgct ctctaccgcc ccctgcaacc ggggcaactg ctccgcctgg tccttcaccc
     1441 cgacccaaac gacccgcgga taccgcaggt ccgggaagaa cccaacccgc tcggcgcgca
     1501 aatgcagcgc gccgaatcct tggcacgcgg cgcgaatcgc ctctcccagc gcctccacgc
     1561 gggccgcgtc cacgtccccc agaaacttca gcgtcaggtg gaattgttcg cgccgggtcc
     1621 agcggacggc gcgttccggc agcgcgcggc gcattttccc ctgcgcctcc tcaatcttcg
     1681 ttttgacctc ttctggtatg gtgaccgcga tgaagagtcg gaactgttcg ctgttggcca
     1741 tgattaaagc ccgttaggga accggctcgc cgcgcgccgc gatgagcaag cgataccact
     1801 cctcgcgcgt caattccagg ctggcggctt tcgtggtttc gcggatacgg tcagggttag
     1861 tggtgccgac aatgggcagg attctggccg gatgcttcag cagccaggcg agtgccacca
     1921 ccgtgcggct agcaccatgc actccggcaa tggcatccac caccggcaag aactgttccg
     1981 ggtggtaggt tttctgggag ggcagcaggc gggttgcgcc gcccccaatc aatcctgctg
     2041 cgagcgggct ccaggccatc ggcgtaatcc gttctatcag gcattgatcc agcgtgccgt
     2101 cggtgaacgc atccagcttg gccaggctga tttcgacctg gtgcgccacc agcggcatcg
     2161 gacaggcggc ttgcagcgcc gttaccaggg ttggacggaa attgctcaca ccgaagtggc
     2221 ggactttgcc ggcggccttg agctgcgaga atgcccgggc aacctcctcg ggatctgcca
     2281 ggtagtcagg ccggtgcagc aggtaaagat caatgatttc cacgcccagc cgtcggagtg
     2341 attgctcgca ggccgcgatg atgtaggaag cggagaagtc gtagcggccc ggagagtcgg
     2401 gttgcgggtc gcctccaggc cggatgccgc ccttggtggt gataacgacc cggtcgcgca
     2461 tgccggagac ttccttgagc acgtcgccga ggatgcgttc cgcctcgccc tggcagtaaa
     2521 tatccgcgtt atcgaacagg gtgtagcccg cttcatacgc cgcgatgatc gcccgccgcc
     2581 cggccgcgcg gcttgcggcg gtgacttcgg cgggggccca agtcccggcc acgcgccagc
     2641 aaccataagc caatcggctg ctcgtcagcg tactcacgcc tagcgggata gtctgcataa
     2701 tctcagtcat gttcgaccga ggttagaacc gtccgtcgcc aggcgcaaag ctaaaaccaa
     2761 tgcggtcgag tgaatcccgc gcttgggcac ccggcaatgc cagttcgcgg attaggcgcc
     2821 ggcacgaggc ggcaaaggag gatcgaggat ggacgttggc gaacggcgcg ggtcgggcac
     2881 agcgcaagtt ggaattcggc ctactgggcc acgcttagga cgttggtttg gccggcgcct
     2941 gcaaggatgg cgctatcctt cttgatggcc acaacccgca tatcgcccag tttgtcgccg
     3001 atgaaaagcg tcttgccgct gatcataacc gatggccgct tggggttgaa gataattgcc
     3061 tgcaaccgaa gcggcggcgg ctggggcggc gggggcggcg ttatgaccgg agcgttggtg
     3121 gcttcgcttt cctggacgtt ggccagcgga tcagcggctg gtgtggatgt ggcattcgcc
     3181 gggagggcgg cggctgtatc ggtgaccagc tccttgggag aagccggttc ctcaactgga
     3241 gcaggttgag ccgcgggggc ggacgccggg gttatcgctt gcggaacagg ggtcgttcgc
     3301 gcgttgacct cgattactga ggccgcgccg cgtgtgcggg tcagctccca ggcaaggagg
     3361 agagtgagga gggctaccgc aaccagcgcg aaaggcagta acaggccgag gccgcggcgg
     3421 gccctctgct gcgcgggttc aactggccga aaggggaggt cgtgtgacgg cagaagaggg
     3481 gtttgctgct gcgcctcttt ggcgcgccgc agggcatcgt tgattaagct catgcgttga
     3541 tgtttccttc aagctcgcgg acggcctgcc cgaccatccg gaaactaatc cgcttgcttt
     3601 gtagcacgta ccccgccagg agggccttgt cgcaaaccgc gttcaccagc cggggaatgc
     3661 ctttgctgta atgatgcaca cgccacaggg ccggcccagt gaaatacggc gtcccgttcc
     3721 cgcccgagac atggagccgg tgctgtacat actgaatcac ctcttgccgc tggagcggcg
     3781 gcaaatggta acgaacagtg atccgctgac gcagttggcg caggcgcgga ttgttcagcc
     3841 ggccgcgcaa ctcgggttgg ccgaataaaa tgatctgcaa gagcttgcgg ttgtcggttt
     3901 ccaggttcga aagcaagcgg acttgttcca gcagctcgtc ggtcaagtcc tgcgcctcgt
     3961 caatgatcag caccgaatcc ttctggcgct cgacctgctt gaggaggaac tcgttgatga
     4021 cggccaacat gtccagccgg tccaagccgc tcaccttcag gccaaattcc agcgcaatcg
     4081 cctttaccaa ttcattggcg ctcatgattg ggttcagaat caaggccgta gagtagcggt
     4141 cgtccagttg ctcgagtacc gcccggcaaa gggtggtctt gcccgcgccg acttcgccgg
     4201 tcatctggac aaagcctttg cgctcccgga tgccgtagag caggtggttg aaggcttccc
     4261 ggtgcttcgg actatagaac aggaaccgcg ggttcggcgt gatgtcgaac ggcggctccg
     4321 tcaggccata gtattccagg tacacgctct tctcgcagca taaagagtgc ctgtgggccg
     4381 taaacggcgg agttaggcgt ggtcaggggc acgggtgtct caaagaaggg cagcatggga
     4441 tcgggaagcg gacgggtgcc gggaaccagc aggtctcctc aaggtccaat agcttgggag
     4501 tggttttgga gtggctattg ggtctcaagc aggtgcttta ccccctgtat accccctgta
     4561 taccccttgt atgtcccctg tattgaatac gcatgtacct ggcttgcctc tcccaacccc
     4621 ctcccaaacg attccgttga gccgcagaaa tacgatgcgc gcgcccagca ccgcccgctc
     4681 acaggcgacc agcgttggcg ggagtttcag tggttcacct attggactgc ctttgcagcc
     4741 ttgaccagcg cggcggcttc ttccggggcg ccgatggtga tgcgcaagta gtcctggacc
     4801 agcgggtcac tgaaccaccg caccagaatc ttccgatccc gcagcttttg gagccactcc
     4861 ttggcgggg
//
LOCUS       DATCGS010000439         9231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_232485, whole genome shotgun sequence.
ACCESSION   DATCGS010000439 DATCGS010000000
VERSION     DATCGS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232485"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1755)
                     /locus_tag="VIL39_09065"
     CDS             complement(124..1755)
                     /locus_tag="VIL39_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY5481614.1"
                     /translation="MNTRKLKKAAVRTGLLWVGVCVAANAMAASTPLLDFNFDEGKGT
                     NVTDSASHLVGLLGTYIDPANDPVVVTDTPSGAAGDKAVSLNFGSATTEGFLVADDST
                     DKVLACTFTNAFTIETWVKYDPTDVRIFEGLGGYGYSWKLGLHYGQIQFTLFAIVDID
                     SGLSMPVDGAWHHVAVVWQPGVGATFYLDGGNETLIANTGSMRAFSDNYLAIGAESIG
                     GNAIQGMIDRFRIHNAILTVDQLDSVAASPKARLGTTLVDFEFTESAPPFSSSAAAAL
                     KASPSNPYVAALTQPTFITDSPSAKTNDYALSFKAGNKATVPDPNVAMALDPADSSFT
                     IQAWLKFGTLPNPEKSVIFFNNAPGGAISFSISTDRHLFVTTFGIADVKSSKAIIPDD
                     GNWHHAAVVHENGKELRFYVDGGLVDTVAYTAGVIFSRTETSFTLGSEGGGNPYVGSI
                     DRFKVAKGVLTADQLDSWPVPGVQPGSPKLTIESSVRVTWPTAPGGYTLQSSTDLGDT
                     KNWTSVTNTPLVDSRGYYMVFPSSAVKVFYRVYKP"
     gene            complement(2177..2656)
                     /locus_tag="VIL39_09070"
     CDS             complement(2177..2656)
                     /locus_tag="VIL39_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481615.1"
                     /translation="MRAYADSSFILRLVTAEADSEAAIAEYRRIGLPPLFFLPLHALE
                     IQNAILQRAFHERRSLPSRARQHIARERDAAFDRLERFLSRRTLLDVALDLDAVIGRA
                     TELATAHTERLGARAIDLLHVASALELESEVFLTTDDRQAQLAKAEGLKIVTVAEAG"
     gene            complement(2653..2895)
                     /locus_tag="VIL39_09075"
     CDS             complement(2653..2895)
                     /locus_tag="VIL39_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481616.1"
                     /translation="MKTITLRTLLREPLTVKRWTRTGKSVQVTDSGEPLWILQPANAA
                     PDEEERRRATVEILDEVLRETPSRISAARLLEDSRR"
     gene            3382..3921
                     /locus_tag="VIL39_09080"
     CDS             3382..3921
                     /locus_tag="VIL39_09080"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY5481617.1"
                     /translation="MTGTRSIHNLALVGFMGTGKSCVGRVVADILHFTFLDTDRVIEA
                     RAGKAVFDIFAQDGEPAFRQWERRIVEELTRRKKTVIATGGGLPMDEANLASLKTHAL
                     VICLWASPESIWKRVRGHNHRPLLNEPDPLGKIRELLAAREPYYRQADVLVNTGMRSV
                     REVALHAVHQFHMAQAAHR"
     gene            3918..4973
                     /gene="queG"
                     /locus_tag="VIL39_09085"
     CDS             3918..4973
                     /gene="queG"
                     /locus_tag="VIL39_09085"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY5481618.1"
                     /translation="MKAALRQQALALDFDDCRVTTANAPESAPRFTQWLVERRHGEMA
                     YLQRTAPKRVDPQQVLPGARSIIMLAVGYHDPNWKSGPIARYARYSDYHYILGERLKQ
                     IADFVNKLGGEGTRSLWYVDTGPLLERDLAQRAGLGFIGRHTNLISRPLGNWIFLSEI
                     ITTLDLEPDAPEKNRCGSCTRCLTACPTAAITAPFQLDARRCISYLTIELKGSIPVEL
                     RPAIGNRVYGCDDCLQVCPWNRFAREGQLMKAHARPDLGQPDLLELLSLDEAEFKRRF
                     AGTPMLRAKRRGLLRNACVALGNIGDERTLPALRKAATDPEPLIAEHAVWAIKQIRMR
                     AAQSSSAVDRARTLGNR"
     gene            complement(5073..7040)
                     /locus_tag="VIL39_09090"
     CDS             complement(5073..7040)
                     /locus_tag="VIL39_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5481619.1"
                     /translation="MDFFERQDQARRNTKLLVVYFILGVAMLTLAVYAAALGILTGVA
                     SSRHHRGYDQETQLVLWNPQIFFGAAIGTLAVIALGSGFKTLELAQGGSTVATMLGGR
                     LVDPTTADPDERKLRNVVEEMAIAAGVPVPQIYLLPQENGINAFAAGHSTSDAVVAVT
                     AGAVRLLNRDELQGVIGHEFSHILNGDMRLNLRLMGAIFGILCLTVIGRVLLYTRSRD
                     SKDKNPLPLLGLALIVIGWIGVVFGRLIQAAVSRQREFLADASSVQFTRNPAGLAGAL
                     KKIGGLSIGSKLESAHAAEASHMYFGNGMGESFFHLMDTHPSLGERIRAIDPSFDGAF
                     PPVSFAEVEREAPRVAPPQRSPLPFPFPGLPRAQGAMAGLMPPVIAAQTVMAGTGNPT
                     PAHLRYAEGLRISIPASLQAAAREGLGASTLVYALLLSDDETVHRKQLDELTAATSAA
                     VCQETLRVLPEVRAVATHAKLPLVDLAIPGLRHLSPTQFQQFRDAVQKLVESDGEIDL
                     FEYVLQKTVLRHLEPYFLQARKPTIQYYALKPLAADCTILLSALAYLGQDEPAKIEFA
                     FQQGAQALSYAAQVQLRLVTAEECELAQVDAALNRLCQAVPQIKKNVLNACAQTVAAD
                     GVIQEMEAELLRAIADTLDCPIPPFIPAQPE"
     gene            complement(7143..7748)
                     /locus_tag="VIL39_09095"
     CDS             complement(7143..7748)
                     /locus_tag="VIL39_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5481620.1"
                     /translation="MFKGLFILVIVVVVPVFFVLMWIVGAYNRLVALRNRFKNAYAQI
                     DVQLKRRYDLIPNLVETAKGYIKHERGTLEAVIAARNAASAANVAAAQNPGDAAAMKG
                     LSGAETALTSTLGRLFAVAEAYPDLKANTTMMALMEELTSTENKVAFARQAYNDSVMG
                     YNTQRETFPTNMIAGPFNFGPAELFVIEKPEEKEAPKVSFT"
     gene            complement(8197..9075)
                     /locus_tag="VIL39_09100"
     CDS             complement(8197..9075)
                     /locus_tag="VIL39_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5481621.1"
                     /translation="MKINLVMAAFLGWFASAQAQPTNSFPLWPDGAPGALGKEDKDIP
                     TLTPYLPDPGKATGAAVVICPGGGYGSLAPHEGGQYARFLNEYGIAGFVLKYRLGSGG
                     YRHPAMLQDAARAVRTVRARAGEWKVDPKRIGIMGSSAGGHLASTLLTHFDAGKPDAA
                     DPIEQQSSRPDLGILCYAVISLGEFGHRGSMNNLLGKDPSPELVRELSNELQVTKDTP
                     PCFIWHTYEDGGVPVENSLQFAAALRKAGVPFDLHIYQKGQHGLGLGTRDWNPEKRHP
                     WTRDCIFWLQAQGFAK"
BASE COUNT         1860 a         2741 c         2858 g         1772 t
ORIGIN      
        1 aatatctgtt ttcataattg ttttcatcga tgattgtgat gccgatctta taattaacgc
       61 ccaattactg ataggaattg ggttcctggc gcgcccgctt gtacttgacg ctgcgcgggt
      121 gaattagggc ttatacaccc gataaaacac cttcaccgcg ctggacggga acaccatgta
      181 atagccacgg gagtccacca gcggcgtatt ggtgacgctg gtccaattct tcgtgtcgcc
      241 caagtcggtg ctgctttgca gtgtatagcc gcccggagcg gtcggccaag tgacccgcac
      301 cgagctctct atcgtcaatt tcggcgagcc aggctgcact cccggaacgg gccaggaatc
      361 cagttggtcg gccgtaagca cgcccttggc aaccttgaaa cggtcaatcg agccgacata
      421 aggattacct ccgccttccg agccgagcgt gaagctggtc tcggtgcggg agaagatcac
      481 gccagcggtg taagccactg tgtcaaccag accgccatcc acgtagaatc ttagttcctt
      541 gccgttttca tgcaccaccg cagcatggtg ccagttgcca tcgtcaggaa tgatggcttt
      601 ggatgattta acatcggcaa ttccgaacgt ggtcacaaac aagtggcggt ctgtggatat
      661 cgagaaggaa atggcgccgc ccggggcgtt attgaagaaa atcaccgact tttcaggatt
      721 gggcagagtg ccgaatttca gccaggcctg gatggtgaac gacgagtcgg ccggatcgag
      781 cgccatcgcc acgttgggat ccgggacagt cgctttattg ccggctttaa agctcaacgc
      841 gtaatcgttg gtcttggcgg aaggcgagtc ggtgataaaa gtgggctgcg tcaaagcggc
      901 cacataggga ttagaaggac tggccttgag agcagcggcg gcggagctgg agaaaggcgg
      961 cgccgattcg gtgaattcaa agtccaccaa ggtggtaccc aagcgagcct tgggcgaggc
     1021 cgccacgctg tccaactgat ccaccgtaag aatggcattg tgaatgcgaa aacgatcgat
     1081 catgccttga atggcgttgc cgccaatact ttctgcgcca atggccaaat agttatcgct
     1141 aaaggccctc atggaacccg tgttcgcgat caatgtctcg ttaccgccgt ccaggtaaaa
     1201 ggtagcgccc acacccggct gccacaccac ggccacatgg tgccaggctc cgtccacagg
     1261 catggacaaa ccgctgtcga tatccacaat ggcaaacaaa gtgaattgga tctgcccgta
     1321 gtggagcccg agtttccagg aatatccata gccgccgagg ccttcaaaaa tccgcacgtc
     1381 ggttggatca tacttcaccc acgtctcgat tgtgaaagcg ttggtgaaag tgcaggctag
     1441 aaccttgtcg gtactgtcat cagccaccag gaatccttca gtcgtggcac tgccgaaatt
     1501 cagactgacc gctttgtcac ctgccgcacc cgagggagtg tccgtaacga ccacgggatc
     1561 gttagccggg tcaatataag tacccagcag gccgacaaga tggctggccg agtctgtgac
     1621 attggtgcct ttgccttcat caaagttgaa gtccagcaag ggggtgcttg cggccatggc
     1681 gtttgccgca acgcaaactc ctacccataa cagcccggtc cgcacggctg cctttttcaa
     1741 ttttctagta ttcatatgtt tgtgtgttta ttgtaacagg tgctgcgggt cgtgtcatgt
     1801 gataaatgta taagaattga gacgggtctt gccggtgtgg cacgcggaat tttttcgcgc
     1861 tgcggcctat ggcgtccggc accggggacg ggcgcggagc agctcgtata ggtggcaatc
     1921 gcggtggaga aggtaccagt taatgagatt gtcatgttct cgcccagtcc cctggtaccg
     1981 ccccactgaa cgcaaggcaa gcgcgatgat ctgcaatgct ccatatgaca ttctcattaa
     2041 ctgacccctt ccttaactga cgccttcccg ccgcttgcgg ccaactgaga ttaaagacgg
     2101 agcgggaact ggcgggacaa taaagcgtgg gagcaggagg cggcaagagc gaaggctgat
     2161 ctcttgcagc catcgctcag cccgcttcgg caactgtgac gatcttgaga ccttccgcct
     2221 tggcaagctg agcctggcga tcgtcggtgg tgaggaacac ctcgctctcg agctccagag
     2281 cgctggctac gtgcagaaga tcaatggccc gcgctccgag tcgttcggtg tgcgcggtgg
     2341 caagctcggt cgcccgaccg atgaccgcgt ccaggtccaa cgcgacgtcc aaaagggttc
     2401 gccgggaaag aaagcgttcg agccggtcaa acgcggcgtc tcgctcacgc gcaatatgct
     2461 gccgcgcgcg agatggtaac gagcggcgtt cgtggaatgc gcgttgcagg atcgcatttt
     2521 gaatctccaa ggcgtgcagc ggaaggaaga acaacggcgg caggccgatc cggcgatact
     2581 cggcaatagc tgcctccgaa tccgcttccg cagtaaccag ccgaaggata aaactgctgt
     2641 cggcgtaggc cctcaccggc gcgaatcctc cagcagacgg gcggcgctaa tcctggacgg
     2701 cgtttctcgc agaacctcgt cgaggatttc gactgtggca cggcggcgct cttcctcatc
     2761 aggagcagcg ttggcaggtt gaagaatcca gagcggctcg ccgctgtcag tgacctgcac
     2821 ggacttgccg gtgcgcgtcc agcgcttgac cgtcagcggc tcccgcagca gcgttcgcaa
     2881 cgtaatggtc ttcatgttgt cagacactag tacccgagag cctgtccgtc accgcgcaat
     2941 tgctaagccg catccatgca gttgaaggga accccattaa aacgcagaga cgcgaaggtc
     3001 ccaacgcggc aaagccgcaa aacggaacag gaagagcaga cagaaaaaca gaagacagaa
     3061 aaatgggatt cctgtttatc tttctgtcac ctatttttct gtcagcaggt gttccactca
     3121 aagtctatga cgaggacggc accctcattg atcggaggag cgctttccct ctttgcaccg
     3181 ctatttcctg acttgctcaa acctgagaag aattaacgca gagacgcaga gggcgcggag
     3241 gaacgcagag gaaaaggact tgttccttct ccttgttctg cgtccttctg cgaacctttg
     3301 cgtctctgcg ttaaatgttc accgcctgca agtccgtaga aactgcgtgc caaagaggcg
     3361 ggaatacgct aactcaataa cgtgactggc acgcgctcta tccacaacct ggccctcgtc
     3421 ggattcatgg gcaccggtaa atcttgcgtg ggccgagtgg tggcggacat cttgcacttc
     3481 actttcctgg ataccgaccg cgtcatcgaa gcgcgcgccg ggaaagccgt cttcgacatc
     3541 ttcgcccagg acggcgagcc ggcctttcga cagtgggagc ggcggattgt ggaggagctg
     3601 acccggcgaa aaaagacggt catcgccacc ggggggggct tgccgatgga tgaagcgaat
     3661 ctagcgagtc tcaagacgca tgcgctagtg atctgccttt gggcctcgcc ggaaagcatc
     3721 tggaagcggg tgcgcggcca caaccaccgg cctctgttga acgaacctga tccgcttggc
     3781 aagattcgcg agctcctggc ggcccgggaa ccctattacc gccaggccga tgtgctcgtg
     3841 aacacgggaa tgcggtctgt gcgcgaagtc gccctgcatg cggttcatca gttccacatg
     3901 gcgcaagcgg cgcaccgatg aaggcggctc ttcggcagca ggcgcttgca ctggacttcg
     3961 acgactgccg cgttaccacg gccaacgccc cggagtccgc cccgcgcttc acccaatggc
     4021 tggtcgaacg gcggcacgga gaaatggcct acctccaacg caccgcgcca aagcgcgtgg
     4081 atccgcagca agtgctcccg ggcgcccgga gcatcatcat gctcgcagtt ggttaccacg
     4141 atcccaattg gaaatccggc cccattgctc gttatgcccg ctacagcgat tatcactaca
     4201 tcctgggtga gcggttgaag cagatcgcgg atttcgtgaa caagctgggc ggcgagggca
     4261 cccgctcgct gtggtatgtg gacaccgggc cgctgctgga acgtgatctg gcgcagcgcg
     4321 ccgggctggg cttcattggc aggcatacca acctcatcag ccgcccactg ggcaactgga
     4381 tcttcctctc cgaaatcatc actacgctgg accttgaacc ggatgccccg gagaagaacc
     4441 gctgcggctc ctgcacgcgc tgcctcaccg catgcccgac ggcagccatc accgccccgt
     4501 tccagcttga tgcgcgaagg tgcatctcct atttgaccat tgagctgaaa ggctccattc
     4561 cggtcgagct gcgcccggcg attggcaacc gcgtttacgg ctgcgacgat tgtctccagg
     4621 tctgtccgtg gaacaggttt gcgcgcgaag ggcaactgat gaaagcgcat gcacgcccgg
     4681 acctgggcca accggacttg ctggagctgc tctcgctgga cgaagcggag tttaagcgcc
     4741 gcttcgccgg cacaccgatg ctgcgcgcca aacgccgcgg cctgctgcgc aatgcctgcg
     4801 tcgccttggg gaacatcggc gacgaaagga ctctcccagc cttgcggaag gcggcaacgg
     4861 acccggagcc cttgattgcc gagcatgcgg tgtgggcgat caagcagatc cggatgcgag
     4921 cagctcaaag ctcgtcagcg gttgaccgcg ctcgaacatt agggaatcgc tgatgagcag
     4981 gccgatgtcg caccagccat aaaagccgcc ccggcattca tcgccagcca cagtcttcag
     5041 aaacacgagc gtccattacg ttccagcctg catcattctg gttgtgccgg gataaatggc
     5101 ggtatcgggc agtcgagcgt gtcggcgatg gcccgcagca gctcggcttc catttcctgg
     5161 atgacgccgt cggcagccac cgtctgggcg caagcgttca ggacgttctt cttgatctgc
     5221 ggcaccgcct ggcagagccg gttcaatgcg gcgtccactt gcgccagctc acactcctcc
     5281 gcggtcacca agcggagctg gacctgggcg gcatagctaa gcgcctgcgc gccttgctgg
     5341 aaggcaaatt cgattttggc cggttcatcc tgcccgaggt aagccagcgc cgagagcaga
     5401 atggtgcaat cagcggccag tggtttgagc gcgtaatact ggatggtcgg cttgcgcgcc
     5461 tgcaggaagt agggttccag gtggcgcaag accgtcttct gcagcacgta ttcgaagagg
     5521 tcaatctcgc cgtcactctc aacgagcttc tgcaccgcgt cgcggaactg ctggaactgg
     5581 gtcggcgaca ggtggcgcag tccggggatg gcgagatcaa ccagcggcag cttggcgtgc
     5641 gtggcgacgg ctcggacttc cggcaacacg cgcagggttt cctggcagac cgcggccgac
     5701 gtggcggcgg tcagctcgtc gagctgtttg cggtgcacgg tttcgtcgtc gctcaagagc
     5761 agggcataaa ccagcgtgct ggcgccgagc ccttcgcgcg cggcggcttg gaggctggcc
     5821 ggaatcgaaa tccgcaatcc ttcagcgtag cggagatggg cgggggtggg attgccggtg
     5881 ccggccatca ccgtctgtgc ggcgataacc ggcggcataa gtccggccat agccccttgc
     5941 gcgcgcggca ggccgggaaa cgggaacggc aggggtgacc gctgcggcgg cgcaacccgc
     6001 ggcgcctctc gttccacctc ggcgaagcta acaggtggga aggctccgtc gaagcttggg
     6061 tcaatggcgc gaatgcgctc ccccagcgac ggatgggtgt ccatcaggtg gaagaacgat
     6121 tcgcccatgc cgttgccgaa gtacatgtgg ctggcttccg cggcatgggc cgactcgagc
     6181 ttcgagccta tgctcaggcc gccaatcttc ttgagcgcgc ccgccaggcc cgccgggttg
     6241 cgcgtgaact gcaccgacga ggcatcggcg aggaactcgc gctgccggct gacggcggcc
     6301 tggatgagcc ggccaaagac cacgccaatc cagccaatca cgatcagcgc cagccccagc
     6361 agcggcagcg ggttcttgtc cttgctgtcg cggctgcggg tgtagaggag cacccggccg
     6421 atgacggtca ggcacaggat gccgaagatg gcccccatga ggcgcaagtt gagccgcatg
     6481 tcgccgttca ggatgtgact gaactcgtgg ccgatgacgc cttgcagctc gtcgcggttg
     6541 agcaacctca cggcgccggc tgtgacggcg acgacggcat cgctcgtgga gtggccggcg
     6601 gcaaacgcgt tgatgccatt ctcctggggc aatagataga tctgcggcac cggcacgccc
     6661 gcggcgatgg ccatttcctc gacgacgttg cgcagcttgc gctcgtccgg gtcggccgtg
     6721 gtgggatcga cgagacgccc gcccagcatc gtggcgaccg tgctgccgcc ctgggccagc
     6781 tccagcgtct tgaacccgct ccccaaggcg attacggcga gtgtgccgat ggcagcgcca
     6841 aagaagatct gcggattcca gagcaccagt tgcgtctcct gatcgtagcc acggtgatgg
     6901 cgcgacgagg cgacgccggt aagaatcccc agcgccgccg cgtaaaccgc cagggtcagc
     6961 atggcgacgc cgagaatgaa atagacgacc aggagtttgg tgttgcggcg agcctggtcc
     7021 tgtcgttcaa agaaatccat gcgcagtgaa acagttacat ggttacaagc gttacaatgt
     7081 tacatgaggc gtttccctat gtaaccgtgt aaccatgtaa ctgatgtaac caatatgaag
     7141 gatcaagtga acgaaacctt cggcgcttcc ttctcttctg gcttctcgat gacgaagagc
     7201 tcggccgggc cgaagttgaa agggccggca atcatgttgg tggggaacgt ctcgcgttgc
     7261 gtgttgtagc ccattacgga gtcgttgtag gcctggcggg cgaaggcgac tttgttctcg
     7321 gtcgaggtga gctcctccat gagggccatc atcgtcgtgt tggccttcag gtcggggtac
     7381 gcctcggcga ccgcgaagag ccggccgagt gtgctggtga gggcggtttc cgcgccggac
     7441 aaacctttca tcgccgcggc gtcgcccggg ttttgagcgg cggccacatt ggcagcggag
     7501 gcggcgttgc gcgcggcaat gacggcttcg agcgtgccgc gctcatgttt gatatagccc
     7561 ttggcggtct cgacgagatt cggaatcaag tcataccgtc gcttgagctg cacgtcaatt
     7621 tgcgcatagg cgttcttgaa gcggttgcgc aacgcgacca ggcggttgta cgcgcccacg
     7681 atccacatca ggacgaagaa caccggcacc accaccacga tcactagaat aaacaagcct
     7741 ttaaacataa gaatcctttc gtttgttgcc taacagctag gcgataccgc cgcagcgggc
     7801 aattacaaac tgagggcggg aatggaagcg ggggccaggg acggagggct ggggagggag
     7861 gggcggcggc gtttgacaag tgcggcccgc tgagggttcc agtggaatcc cgtggccaaa
     7921 tccgaagccc gaaggtcgaa ggccgaaaga aggccgaagt ccgaggtccg aagagaccaa
     7981 actccggtcc gtccacccgg ccctgtcagg caccaaaagc agcaggccca agcgctccgg
     8041 gtcattccat ttctatgatg gcaagaaggt tggagctttc gggtttcggg tttcgacctt
     8101 ctttcggcct tcgaccttcg ggcttcggat ttccaccgcc atgggttcgc ccactgaaac
     8161 cgtcctggcc tcccccttca ctcgccccgt cttcttctat ttcgcgaagc cctgcgcctg
     8221 cagccagaag atgcagtcgc gggtccacgg atgccgcttc tcggggttcc aatcgcgcgt
     8281 gcccaggcca aggccatgct ggcctttttg gtagatatgc agatcaaacg ggaccccggc
     8341 tttgcgcagc gcggcggcaa actgaaggct gttctccacc ggcacacccc cgtcctcata
     8401 agtgtgccag atgaagcagg gcggcgtgtc tttggtcacc tggagttcat tggacagctc
     8461 gcgcaccagt tcgggcgagg ggtctttgcc cagcagattg ttcatagatc cccggtgccc
     8521 gaattcgccc aggctgatga ccgcgtaaca gaggatgccc agatcagggc gggaactctg
     8581 ctgctcgata gggtctgcgg cgtccggctt gcccgcgtcg aaatgcgtca gcagcgtgga
     8641 ggcgaggtgg ccgccggctg aagagcccat gataccgatg cgcttggggt ccaccttcca
     8701 ttcgcccgcc cgggcgcgca cagtcctcac cgcgcgcgca gcgtcctgca gcatggccgg
     8761 atgccggtag ccgcccgagc ccagccggta tttcaacacg aacccagcga tgccgtattc
     8821 gttcaggaag cgcgcatatt ggccgccttc gtgcggggcc aggctgccgt agcctccgcc
     8881 cgggcagatc acaacggcgg cgccggtcgc cttgcctggg tccggcaagt acggcgtgag
     8941 cgtggggatg tctttatcct ctttgccgag cgcgcccgga gcgccatccg gccagagcgg
     9001 gaacgaattc gtcggctgcg cttgagccga cgcgaaccat ccaaggaatg cggccataac
     9061 aaggttgatt ttcatcgcga cagcatcata cccatccgcg gagggcgtgg gcgattcaaa
     9121 tcaccttcct ggaaacagga cggggtggag tgaacaacgc gcaagctcag gcgtcaaaca
     9181 acaggcgcca gagcgagggt gcctggccgt cgccgagacg gtcgcgagac c
//
LOCUS       DATCGS010000440         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_232538, whole genome shotgun sequence.
ACCESSION   DATCGS010000440 DATCGS010000000
VERSION     DATCGS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232538"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIL39_09105"
     CDS             complement(<1..351)
                     /locus_tag="VIL39_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481622.1"
                     /translation="MLLGATLMGALSCSIAAQSQADAAKPGSQKPVSVLPLEQSASRG
                     ITTRDPSSIAKCKDEYWVFYTGRGVPSYRSRDLVKWERGPAVFKIAPEWIAKIVPENR
                     NLQYWAPDIIKLGDR"
     gene            355..672
                     /locus_tag="VIL39_09110"
     CDS             355..672
                     /locus_tag="VIL39_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5481623.1"
                     /translation="MAERATYFGLHPRQYGAAWAKAQGLQVSPHGWGSLVGFYMTLQV
                     GRAITNYYRGENHPLDNELLIADGYAIQGGTASMSEAPGFGLKLDEEKFAAAIKPKFD
                     LKV"
     gene            845..2200
                     /locus_tag="VIL39_09115"
     CDS             845..2200
                     /locus_tag="VIL39_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481624.1"
                     /translation="MTWNSMNRRQFLKRSSASLLALSAATYVPTIFAAEKTRRVGLIG
                     AGWYGKSDLWRLVQVAPVEIVSICDPDKHMLAEAVEIASQRQKSRQRPRTYGDYREML
                     KEKDLDIVLVGSPDHWHALHAIAAMEAGADVYCQKPISRDVREGEAMLDAARKHKRVV
                     QIGTQRKSTPHLIDVKKQVVEAGLLGKVGHVEMCCYFHMRANGNPPLEPVPDYLDYEM
                     WTGPAPLRPYDGLPHKRWWRTFTEYGNGIMGDMCVHMFDTARWMLHLGWPKRISSAGG
                     IFVQKEGKSNITDTQTATFEYDGFDAVWQHRTWGTTPDPDYPWALFIYGEKGTLKAST
                     MRADFIPIDGKAKPLHFECVFEREQYPEDVNEKDIELNAAPATRRHMRDFLAAIDQRG
                     RPVADIEEGHISTASSILANVAMEIGRPIVYDPQKRVAVNDDDATRRLRRAYRGPWIH
                     P"
     gene            complement(2298..3083)
                     /locus_tag="VIL39_09120"
     CDS             complement(2298..3083)
                     /locus_tag="VIL39_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481625.1"
                     /translation="MKTRRKESCAFTLIELLVVIAIIAILAALLLPALSSAKTKAQQI
                     RCVSNLRQLSTAAIMYQQDTGRSIDYNVTAKLWMQTLIDYAIRINDNRLCPVAFGRTP
                     APPDPTAGTAAAAWIWNNDPKLTGSYSINGWLYYYDTKPDGVSTWVTDRAKFFQKDTS
                     IAMPVLTPFFMDAIWPDTWPEIGDTPATDLFLGNVNSALGRISIARHPLMRQKAVTGQ
                     HLPSAINISYVDGHSSKIPLQKLKTVYWHVGYVPVDDVWKTSP"
     gene            complement(3219..>4012)
                     /locus_tag="VIL39_09125"
     CDS             complement(3219..>4012)
                     /locus_tag="VIL39_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481626.1"
                     /translation="YLAHLKPTRIHDVDYVWGKADSTHGMLWVVGRDPDLFDYFMPER
                     WRRTPSKRLSSTNETYYTLTKDRINLVWKFSRVGEPPSRNAAIPRSGERPALGFNSPF
                     EEVAVALDLQRKGVDTTYPRAIYMSGLESTRSGLYASDDRRYRSHQELTTPDGQPVLR
                     SDHNYLTIWGFWNGLDEILAGKDEAYCQGVNLHEARQNGIINAREAKQLLEREQRQLA
                     AAGYRDLLPKPTHYLLSLRHSGSLVLNKDGKPAVRVCSFALIQKR"
BASE COUNT          790 a         1208 c         1212 g          802 t
ORIGIN      
        1 ccggtcaccc agcttgatga tgtcgggagc ccaatattgc aagttgcggt tttctggaac
       61 aatcttggca atccattccg gtgcgatttt gaatacggcc ggcccgcgtt cccatttcac
      121 caggtccctg gaacgatacg aaggcacgcc ccggcccgtg tagaagaccc agtattcatc
      181 tttgcatttc gcgatgctgg aaggatcccg ggtggtaatg ccgcgacttg ccgattgttc
      241 cagcggcagg acggaaacgg gcttttgcga gcccggtttg gctgcatcag cttgcgactg
      301 cgctgcgatt gaacaggaca acgctcccat caacgtggca cccagcagca agcgctggcc
      361 gagagggcaa cttattttgg gcttcatcca cgccagtatg gagcggcttg ggccaaagcg
      421 caaggcttgc aggtctctcc gcacggctgg ggctcgttgg tcggtttcta catgaccttg
      481 caggtgggcc gggcaatcac gaattattac cgtggcgaaa accacccgct ggacaacgaa
      541 ctgttaatcg ccgacggcta tgcgattcag ggtggaaccg ccagcatgtc agaggcgccg
      601 ggatttggct tgaagttgga cgaagagaag tttgcggcgg caattaagcc caagttcgat
      661 ctgaaggtat gagcgacgag ctgaagttgg gaaagcagga gtccagaggg tgagaatcct
      721 ttggcgcagg ggcctgggga cggcgcgagt ccccagccgg agcgccggaa cgaccttcca
      781 acttgccaac ccagcccatg gcacggccca cgccagtcga gacttgcctt cctctccctc
      841 ttcgattaca tggaacagca tgaaccgccg ccaattcctc aagagaagtt cagcctcgct
      901 cctcgcctta tccgccgcca cctacgtgcc gacgatcttt gccgctgaga agacccggcg
      961 ggtcggcctc atcggcgcag gttggtatgg caaaagcgac ttgtggcgcc tggtgcaagt
     1021 cgccccggtc gaaatcgttt ccatctgtga tccggataaa cacatgctcg ccgaggccgt
     1081 cgagatcgcc agccagcgcc aaaagtccag gcaacggccg cgcacctacg gcgactaccg
     1141 cgagatgctc aaagagaagg atctcgacat cgtcctcgtc ggttcgccgg accattggca
     1201 cgcgctgcac gccatcgccg ccatggaagc cggcgccgat gtctattgcc agaaacccat
     1261 cagccgcgac gtgcgcgaag gcgaggccat gctcgacgcg gcgcgcaaac acaagcgcgt
     1321 agtgcaaatc ggcacccaac gcaagagcac cccgcacttg atagacgtga agaagcaggt
     1381 cgtcgaggcc gggttgctcg gcaaggtcgg ccacgtcgaa atgtgctgct atttccacat
     1441 gcgcgccaac ggcaacccgc cgctcgagcc cgtgccggac tatctcgatt acgaaatgtg
     1501 gaccggcccg gcgccattgc gcccgtacga tggcctgccg cacaaacgct ggtggcgtac
     1561 cttcaccgaa tacggcaacg gtatcatggg cgacatgtgc gtgcacatgt tcgacaccgc
     1621 ccgctggatg ctccatctcg gctggcccaa acgcatcagc tcggccggcg gcatcttcgt
     1681 ccaaaaggag ggcaaatcga acatcaccga cacccagacc gccaccttcg agtatgacgg
     1741 tttcgatgcc gtatggcagc accgtacctg gggcacaacg cccgatccgg attatccctg
     1801 ggcgctcttc atctacggtg agaaaggcac actcaaagcc agcaccatgc gcgcggactt
     1861 catccctatc gacggcaagg ccaagccgct ccatttcgag tgcgtttttg agcgcgagca
     1921 atatcccgag gacgtcaacg agaaggacat cgagctaaac gccgcgcccg ccacccgccg
     1981 ccacatgcgg gatttcctgg cggcgattga tcagcgcggg cggccagtcg ccgacatcga
     2041 agaaggccac atctcaaccg caagcagcat cctggccaac gttgccatgg agatcggccg
     2101 cccgattgtc tatgacccgc agaagcgcgt ggccgtgaac gacgatgacg cgacgcgtcg
     2161 gctccggcgc gcttatcgcg ggccctggat ccatccttga gcaagggtca catccgccac
     2221 gtcatcgcgt cgaaggcccg ctactggcct cccggtttta ccaggtcatt accagtgctt
     2281 tcgcttcgca cctggaatca gggtgatgtt ttccagacgt cgtccaccgg cacgtagccg
     2341 acgtgccaat agacggtctt gagcttctgc agagggatct tacttgaatg gccgtcaacg
     2401 taactgatgt tgatggcact tgggagatgc tggccggtta cggctttctg gcgcatcaat
     2461 gggtgccgag cgatggagat tcgccccagg gccgaattca catttccgag gaacaggtcg
     2521 gtggcgggag tgtcaccgat ctcgggccat gtgtccggcc agatggcgtc catgaagaac
     2581 ggagtcaaca cgggcatcgc aatggaagtg tccttctgga agaactttgc ccggtcagtg
     2641 acccaagtgc tgacgccgtc gggttttgta tcgtagtagt atagccagcc gttgatggag
     2701 taactgccgg tcagtttggg atcgttgttc cagatccagg cggctgcggc agtcccggcg
     2761 gtcgggtctg gcggcgccgg cgtgcggccg aacgcaaccg ggcaaagccg gttgtcattt
     2821 atcctgatcg cataatcaat gagggtctgc atccacagtt ttgcggtgac gttgtaatcg
     2881 atggacctgc cggtgtcctg ctgatacatg atggcggcgg tgcttaattg ccggagattg
     2941 ctgacgcacc ggatttgttg cgccttggtt ttcgccgagg atagcgccgg caacaggagg
     3001 gcggcgagga tggcgatgat ggcaatgacc acgagcaatt cgatcaacgt aaacgcgcac
     3061 gattcttttc ttcgcgtctt cataatagtg cccgggggga acgctacttt aactggttca
     3121 gccgggcaat ggctattgtc tcaacgtggc ggccgattcg attctcggtt ctcgcagatc
     3181 cccttcacca ggggggctca gccatgttcg tgattgtctc agcgcttctg gattagggca
     3241 aaactgcaga cccgcaccgc tggcttcccg tccttgttca ggaccaggct ccccgagtgg
     3301 cgaagcgaaa gcagataatg agtcggcttg ggcaacaggt cccggtagcc tgccgcggcc
     3361 agttgcctct gttcacgttc cagcaactgc ttcgcctcgc gggcgttgat gatgccgttt
     3421 tgacgtgcct cgtgcaggtt gactccttgg cagtaggcct cgtctttgcc ggccaggatt
     3481 tcgtccagcc cattccagaa tccccagatc gtcaggtaat tatggtctga ccgcagcacc
     3541 ggctggccgt ccggcgtggt cagttcctgg tgcgatcggt accggcggtc gtccgaggca
     3601 tatagtcccg aacgcgttga ctccagcccc gacatgtaaa tcgcacgggg gtaagtggta
     3661 tccacgccct tgcgctgcag gtccagcgcc accgctacct cctcgaaggg gctgttgaag
     3721 cccaaggccg gccgctcgcc gctccgggga atggctgcgt tccgggaggg cggctccccc
     3781 acgcgcgaga atttccatac cagattaatg cggtccttgg tcagggtgta gtaggtttcg
     3841 ttggtcgagc tgagccgttt gctcggcgtg cgccgccagc gctccggcat gaaatagtca
     3901 aacaggtccg ggtcccgtcc cacgacccag agcatgccat gagtgctgtc ggccttgccc
     3961 cagacatagt ccacgtcgtg gatccgggtg ggcttgaggt gcgcgagata gt
//
LOCUS       DATCGS010000441         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_484087, whole genome shotgun sequence.
ACCESSION   DATCGS010000441 DATCGS010000000
VERSION     DATCGS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484087"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VIL39_09130"
     CDS             complement(<1..475)
                     /locus_tag="VIL39_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5481627.1"
                     /translation="MSDLLQLVVSEGASDLHIRVGAPPVIRVHGILHRVEGPSLRPED
                     SEELMRSITSEDHIQGVRERGGADFGFAFGDMARFRVSVFKAKGDFGIVLRQIPTKML
                     TMEEIGIPPSAKELLYKPRGLVLVTGPTGCGKTTTLASMINIINEEREEAHIITIE"
     gene            complement(667..2385)
                     /locus_tag="VIL39_09135"
     CDS             complement(667..2385)
                     /locus_tag="VIL39_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5481628.1"
                     /translation="MAEKDDYLAETLIDLGFVSAEKVAEARQEAQAAGVGVVDLLVAN
                     KVLRAEDVTQAKAAQFGAEVVNLGELKIADDVIATIPRHIARKYRVVPVFKNDNSITI
                     AIADPSDLDTIDSLGHLLNGAQIELRVASEADIESALSKYYAERGGGVVTDPRLKEAI
                     EDLTREHVEIEAAMSGDGAVVEADAPLIKLVNSIISEAFKMRASDIHLEPLAKTFRMR
                     YRIDGVLHEVKAPPKRLQAAVISRLKIQSNMSISEHRIPQDGRIQSSAGGKLIDLRVS
                     CLPTNHGESIVMRILDKEGLRLGLPELGFFTDDQQTFERLIGLPDGILLVTGPTGSGK
                     TTTLYSCLHFINRPDRKIITVEDPVEYLLSGINQVQVSEAVGLTFSFALRSILRQAPN
                     VIMIGEIRDLETATIAINASLTGHLVFSTLHTNDAPSAVTRLIDIGVKPFLVASSTRA
                     LMAQRLVRKVCKQCAAPCLPTEAEMRTLGLDAASLQGATFQKGKGCSNCNNTGYRGRF
                     GIFEIFVIDDEARKLIYEKVSSSVLRTRAREMGMRTLREDGVRKTQAGLTTPDEVIRA
                     TVGDVD"
     gene            2690..2986
                     /locus_tag="VIL39_09140"
     CDS             2690..2986
                     /locus_tag="VIL39_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481629.1"
                     /translation="MQYDPENTLVVVNGSGEIRDGDASAQTAEAIYFLNQNQSNGVLV
                     DFSDALSEVSLPRLYRLPDYFSELGAPWNVRIAVLLPRTAYRLETYHARNSFCL"
     gene            3017..>3413
                     /locus_tag="VIL39_09145"
     CDS             3017..>3413
                     /locus_tag="VIL39_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY5481630.1"
                     /translation="MKPMPASPSGSLARLTSLDAFRGLVILTMTFVNYLAGVKAIPAW
                     AKHLPPDQDGYTFVDVVFPGFLFIVGVAIPLALHQRMARGESLFSLLQRVLARSASLL
                     FVGVIMVNSSYYSAQATGMSGSLWFLLAML"
BASE COUNT          633 a         1069 c          978 g          733 t
ORIGIN      
        1 cttcgatggt gatgatgtgc gcctcttcgc gttcctcgtt gatgatgttg atcatcgaag
       61 cgagcgtggt cgtcttgccg caaccggtgg gtcccgttac cagcaccaac ccgcgcggtt
      121 tatagagcag ttcctttgcg ctcggcggaa tgccgatttc ctccatcgtc agcattttgg
      181 tcggaatctg ccggagcacg atgccgaagt cgcccttggc cttgaagacg ctcacgcgaa
      241 accgcgccat gtcgccgaag gcaaagccga agtcggcgcc gccccgttcg cggacgccct
      301 gaatgtgatc ttcggaggta atgctgcgca tcagctcctc gctgtcttcg ggcctcagcg
      361 acggcccttc cacccggtgc agaatgccgt gcacgcgaat cacgggtggc gcacccacgc
      421 ggatgtgcag gtcggaagcc ccttccgaca ccaccaactg caacaaatca gacatcgagt
      481 aagacattgc ttaaatccag tcagacgcgc atcgttctcg cgacacgcat ttcaattcca
      541 tacctattct accatgaagg atggacagtc gtgaagccca tttgtcacgc tgccccgggt
      601 tgatctgcgg catcacagca cgcccccatt caactcacgc aaactctctg ccttcagacc
      661 gaccatttaa tccacgtctc ccaccgtcgc gcggataacc tcgtccgggg tggtcaatcc
      721 ggcctgggtt ttgcggacgc cgtcttcgcg cagggtgcgc atgcccatct cgcgggcgcg
      781 ggtgcgcaag acggaggagg agaccttttc atagatgagc tttcgcgctt catcgtcaat
      841 gacgaagatc tcgaagatgc cgaagcggcc gcgatagccg gtattgttgc agttggagca
      901 gcctttgcct ttctggaagg tcgccccctg gaggctggcc gcatccagac ccagcgtgcg
      961 catttccgct tccgtcggca ggcacggggc cgcgcactgc ttgcacactt tccgcaccag
     1021 ccgctgggcc atcagagccc gggtggacga ggccaccagg aacggcttga cgccaatatc
     1081 aatgagccgc gtgacagcgc tcggggcgtc gttggtgtgg agcgtggaaa agaccaagtg
     1141 accggtcagc gaggcattga tcgcaatggt ggcggtctcc aggtcgcgga tttccccgat
     1201 catgatcacg ttgggcgcct ggcgcaggat cgagcgcaag gcgaaggaaa aggtcagccc
     1261 gacggcttca ctcacctgca cctggttgat gcccgacagc aaatactcca ccgggtcctc
     1321 gaccgtgatg atcttccggt ccggccggtt gatgaagtgc aggcaggaat acagggtggt
     1381 cgtcttgccc gagccggtcg gcccggtcac cagcagaata ccgtccggca atccaatgag
     1441 ccgctcgaag gtttgctggt cgtcggtgaa aaagcccagc tccggcaagc ccaaacgcag
     1501 cccttccttg tccaggatac gcatgacgat gctttcgccg tggttggtcg gcaaacagga
     1561 cacgcgcagg tctatcagct tgccgcccgc cgaagactgg atacggccat cctgcggaat
     1621 ccggtgctcg gaaatggaca tgttggactg aatcttcaag cggctgatca ccgccgcctg
     1681 caagcgtttg ggcggagcct tcacctcgtg cagcacaccg tcaatccggt agcgcatgcg
     1741 aaacgtcttt gccagcggct ccaggtgaat gtccgaagcc ctcatcttga atgcttcgga
     1801 aatgatcgag ttgaccagct tgatcagcgg ggcgtccgct tcgaccaccg cgccgtctcc
     1861 cgacatggcc gcttcgattt caacgtgttc acgcgtgaga tcctcaatgg cctccttgag
     1921 gcgcggatca gtcaccacgc cgcccccccg ctccgcataa tacttgctca gcgccgattc
     1981 aatatccgcc tcggaggcga cgcgcagctc gatctgcgcg ccgttcaaca gatgccccag
     2041 gctgtcaatc gtgtccaggt ccgacgggtc cgcgatggcg atagtgatac tgttgtcgtt
     2101 tttgaagacc ggcacgaccc ggtattttct ggcaatgtgc cgcgggattg tggcaatgac
     2161 atcatcggca atcttcagtt cgcccaggtt gacgacctcg gcgccgaact gggcggcttt
     2221 ggcctgggtg acatcctcgg cccgcagcac cttgttggcc accaggaggt ccacgacgcc
     2281 cacgccggcc gcctgggctt cctgccgcgc ttcagccact ttttcggcgg acacgaaccc
     2341 gaggtcaatc agtgtctcag ctaaataatc gtctttttca gccacgttct attttgcact
     2401 acacacgttc gtcgtggaaa tgtgtccagc agagtaccgg ggaaacccgc gatgtcaatg
     2461 ctcgcgctga gaaatggaaa taattgcgcc ggcccgcgag ggcgtcggct gccgccctgg
     2521 attggcgctg gcggcgcctt ctttcaccgc ggcaactaaa gcgcccgcat ccgtgtaagg
     2581 caaataccta catagttatc cgccgtttcc tgactattca atctggccgc cgcaggtgtt
     2641 aaatgaggct caattctgta acacgagaga gattggtatg ccttggacag tgcaatatga
     2701 tcctgaaaat actctggttg tggttaatgg ttctggcgaa ataagggacg gggatgccag
     2761 tgcgcagacc gctgaagcca tctatttcct gaaccagaac cagtcaaacg gtgtcctggt
     2821 ggatttttcg gatgccctgt cagaagtatc tctgccccgg ctgtatcggc tgccggatta
     2881 tttctccgaa ctcggcgcgc cctggaacgt ccggattgcg gtgctgttgc cccggactgc
     2941 ataccggctt gagacgtatc acgccaggaa ctccttctgc ttgtaatcag ccggatcctc
     3001 gtccagtctg ccgcgcatga agccaatgcc ggcctcccca tctggttctc tcgctcgcct
     3061 gacctccctc gacgctttcc ggggcctggt gatcctgacc atgaccttcg tcaactacct
     3121 cgccggggtg aaggcgattc cggcctgggc aaagcacctg ccgccggacc aggacggcta
     3181 caccttcgtg gatgtcgtgt tccccggctt cctgttcatc gtcggcgtgg cgattccttt
     3241 ggcgctgcac cagcgcatgg ctcgcggtga gtccctcttc tccctgctgc agcgggtgct
     3301 cgcgcgctcg gcctcgctcc tgttcgtcgg tgtgatcatg gtcaactcga gttattactc
     3361 cgcccaagcc accggcatga gcgggagcct gtggtttcta ctggccatgc tgg
//
LOCUS       DATCGS010000442         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_232699, whole genome shotgun sequence.
ACCESSION   DATCGS010000442 DATCGS010000000
VERSION     DATCGS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232699"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2720)
                     /locus_tag="VIL39_09150"
     CDS             complement(<1..2720)
                     /locus_tag="VIL39_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY5481631.1"
                     /translation="MASRTSPTNMGLALLANLAACDFGYLSVGQLLRRTHDALGTMQR
                     LERHRGHFFNWYDTRTLKPLAPLYISSVDSGNLAGHLLTLSPGLAELAAQPILAPQLF
                     SGLCDTVGILRELVGRSSELEQLEAELAQPPSGLRAGFDLLQRVTHRASALAAAPGDG
                     AGEELKWWTQALERNCQDHLEDLLFLAPWLTLAPDSVAQISGKLHQAPTLRQVSELER
                     SLCPSLEAALHTLSVKPTSSRKPEEEAGLAQWLRLLREASSRARQRILVLETLARQST
                     ELADIDFRFLFDPARELFSIGFNVTERRYDASFYDLLASEARFCSYVVIALGQVSQDH
                     WFTLGRLLVTLRGDLVLASWSGSMFEYLMPLLVMPNYENTLLDQTCKGAVRQQIKYGQ
                     LRGVPWGVSESGYNRTDVHLNYQYRAFGVPGLGLKRGLAEDLVIAPYATAMALMVAPR
                     EACGNLEQLAAEGREGAYGFYEAVDYTPSRLPPGQSSVTIRSFMAHHQGMSLLALAYR
                     LLDCPMQRRFLSCPSFRAADLLLQERVPQTAAKVLSDEYELVKSRKVPDDGDGLMRVF
                     TNPTLHAPEVHLLSNGRYHVVVSNAGGGYSRWREFAVTRWREDATRDCWGTFIYVRDL
                     ATDKFWSAAQQPCGCATKRCEAIFTQARAEFRQHHAGLEVHTEICVSPEDDVELRRVT
                     LTNHSNAPRVIELTSYAEVVLALPAADASHPAFSNLFVQTEFLRPRPAVLCTRRPRAE
                     GEKPPWLLNFMVGQGGQQGEVSCETDRARFIGRGRTLAEPAALQTISPLSNTTGSVLD
                     PISSLRRIVILAPHETARVDLVMGMAENRDAALALIEKYYNPRMTDRALDLAWTHSQV
                     TLRHLNASEADAQLYARLSSPLIYASPARRASPSVLLNNRRGQSGLWGYG"
BASE COUNT          599 a          827 c          976 g          530 t
ORIGIN      
        1 atgccatagc cccaaagtcc gctttggccg cggcgattgt tgagcaatac gctgggagag
       61 gcacgccggg ccgggctggc gtaaatcaag ggactcgata gtcgagcata gagttgcgcg
      121 tcggcttcgg aggcattgag gtggcgcagg gtgacttggc tgtgagtcca cgcgaggtca
      181 agcgcccgat cggtcatgcg cgggttgtag tatttttcta tgagcgccag cgctgcgtcc
      241 cggttctccg ccatgcccat gaccagatca acgcgggcgg tttcatgggg ggccaatatg
      301 actatgcgcc gcaatgagct gataggatcc aagacggaac cggtggtgtt ggacagcggt
      361 gagatggtct gcagagcggc tggttcggcg agggtccggc ctcgcccgat gaatctggcg
      421 cggtcggttt cgcaggagac ttcgccctgc tggccgccct gccctaccat gaagttcaga
      481 agccagggcg gcttttcccc ttcggcgcgg gggcggcggg tgcaaagcac ggctgggcgt
      541 ggtcgcaaga actcggtttg cacgaataga ttgctgaagg cgggatggga agcgtccgct
      601 gcgggaagcg cgagcacgac ttcggcataa ctggtcagct caatgacgcg cggcgcgttg
      661 gaatggttgg tgagagtcac gcggcgcaac tcgacatcgt cttcgggcga gacgcagatt
      721 tcggtgtgaa cttccaggcc ggcatggtgc tggcgaaact cggcgcgggc ttgagtgaaa
      781 atggcttcgc aacgtttggt ggcgcagccg caaggttgtt gggcggcaga ccagaatttg
      841 tcagtggcca gatcgcgcac atagatgaaa gtgccccagc aatcgcgcgt ggcatcttcg
      901 cgccagcggg tgacggcgaa ctcccgccag cggctatagc cgccgcccgc gttgctgacc
      961 accacatggt agcggccgtt ggagagcaga tggacttccg gcgcgtgcag agtgggattg
     1021 gtgaagacgc gcatgaggcc atcgccgtca tccggcacct tccgcgactt aaccaattca
     1081 tactcgtccg aaaggacctt ggcagcggtt tggggcacgc gctcctgcaa gagcaaatct
     1141 gcggctctga acgacggaca cgacaggaag cgccgttgca tgggacagtc cagaagccga
     1201 tacgccagag cgagcaggct catcccctga tgatgggcca tgaacgagcg gatggtgacg
     1261 ctggattggc ctggaggcag ccgtgaaggg gtataatcca cggcttcgta aaatccgtaa
     1321 gccccttcgc ggccttcggc ggcgagttgt tcgaggttgc cgcaggcttc ccggggcgca
     1381 accatcagcg ccatcgctgt ggcgtaagga gcaatcacga ggtcctcggc caggccgcgc
     1441 ttcaaaccga ggcccggcac gccaaaggcg cggtattggt aattcaggtg gacgtcggtc
     1501 cgattgtagc ctgattcgga aacaccccac ggaacgccgc gcaattggcc gtacttgatt
     1561 tgctgccgga cggccccttt gcacgtttga tccagcaagg tattttcgta attcggcatg
     1621 accagcagcg gcattaagta ctcgaacatc gatccgctcc aggaagccaa caccagatcg
     1681 ccgcgcaaag tgacgagcag acggcccagc gtgaaccaat ggtcctgcga aacctgtccc
     1741 agggcaatca cgacatagct gcagaaacga gcctcggacg ccagcagatc ataaaagctg
     1801 gcgtcatagc gacgttcggt gacgttgaag ccgatggaga agagctcgcg cgcgggatcg
     1861 aaaagaaagc ggaagtcgat atcggccagc tccgtgctct gccgagccaa ggtttccaga
     1921 acaagaatgc gctgccgggc gcgactgctg gcttcccgca ggaggcgcaa ccattgggca
     1981 aggcccgctt cctcctctgg cttccgggat gaggtcggct tcacggagag ggtgtgcaac
     2041 gccgcctcga gggatgggca gagagatcgt tctaattctg atacctggcg caacgtgggc
     2101 gcctggtgga gtttcccgga aatctgcgct acggaatcag gcgctaatgt cagccaaggc
     2161 gccaggaaga gcaaatcttc caaatgatcc tggcagtttc gttccagagc ctgggtccac
     2221 catttcaatt cctccccggc cccgtctccg ggggcagcgg caagggcgga cgcccgatgg
     2281 gtcacccgtt gcaacaaatc aaagcctgcg cggaggccgg agggcggctg cgccagctcc
     2341 gcctcgagct gctccaactc agaactcctg ccgaccagct cgcgtagaat tcccactgtg
     2401 tcgcaaaggc cggagaaaag ctgcggagcg aggatgggtt gggccgctaa ttccgccagg
     2461 ccggggctta gggtcagcag atggccggcg aggttgccac tgtccacgct ggaaatatag
     2521 agcggcgcca agggtttgag ggttcgggta tcataccagt taaagaagtg gccacggtgc
     2581 cgttccaggc gttgcatggt gccgagggca tcgtgtgtgc gccgcaggag ttggccaacc
     2641 gacagatagc cgaagtcgca cgcggccagg ttggccagca gggccaagcc catgttggtg
     2701 ggcgaagtgc gtgaagccac caccggaacg ggctcttcct gaaagttatc cggcggcagc
     2761 cagtgttctt gtgctgtgac aaaggtttca aaaaagcgcc aggtcttgcg cgccgttcgc
     2821 cgaaggaaag ctaattgctt cggttttaac tccgccaccg ccgaaggctc gatgggctgg
     2881 ctgatgcgcc aggctatcca aggagaggct agccagaggc ccaggatcgg cg
//
LOCUS       DATCGS010000443         3918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_533636, whole genome shotgun sequence.
ACCESSION   DATCGS010000443 DATCGS010000000
VERSION     DATCGS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533636"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VIL39_09155"
     CDS             <1..641
                     /locus_tag="VIL39_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481632.1"
                     /translation="ANAKEKAKRTACKSNMRQAILSVHMYANDWRDYVPDGRDNDDQW
                     HAIRIRNDTYTNLVQYTGNLKIMDCPNFTYGSFTRYSATWGYLIGYAYLGHALDKNNS
                     SFWLTTSPYYWRSPLKTTESPTNYIIADANTWGGGLLMAPHCKTGPYQRTSPGSPNVA
                     ATFINDATTETPQSVGAVGGNVGLLDGSVSWKRMKQMKQQFASSYVIYWGNW"
     gene            complement(724..1092)
                     /gene="queD"
                     /locus_tag="VIL39_09160"
     CDS             complement(724..1092)
                     /gene="queD"
                     /locus_tag="VIL39_09160"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY5481633.1"
                     /translation="MRMELRKTFQFEAAHLLPHLPRSHKCRRLHGHSFKVEIVVAGEC
                     DPRLGWLMDYADISRAFKPIWERLDHYYLNEVPGLANPTSENIAVWIWKKLKPRLPLL
                     TEVAVAETCTAKCVYRGEQS"
     gene            complement(1089..2450)
                     /locus_tag="VIL39_09165"
     CDS             complement(1089..2450)
                     /locus_tag="VIL39_09165"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEY5481634.1"
                     /translation="MNLPNYFLADLPPEATLSAAMIGEACQTLKRNRERYLAHRSSHS
                     LVKTLSDVARSWLEPDYSFRKLALERGPAATGFSSATLARGLDSFFKLLTQEHFHVLL
                     EQDLGHGHRLDEMVAGEAERKSGRAAIAIAPELLAQITAGNLPNPTLAAMVLGVLVRS
                     AQFVKCASGTSLLPRLFAHSLYQVEPKLGACLEVAEWRGGNADLEKALFDEADCVTAT
                     GTDETLAAIRQRVPAKARFLGYGHRVSFGYIAGGALTGLHARKVAWQAAADVTAWNQQ
                     GCLSPHVIYVEHGGGVPPEQFAEMLAEELERREESEPRGDLPVQSAAAIASRRSFYEV
                     RAAHSPDTRHWCSRNSTAWTVVYEADPRFQLSCLNRFIYIKGVKDMTEALQNAESVRG
                     QVSTVGLAAPEDKAQPLATELARWGVTRVCRLGQMQEPPLAWRHDGRPALGDLVTWTD
                     WEM"
     gene            complement(2447..3721)
                     /locus_tag="VIL39_09170"
     CDS             complement(2447..3721)
                     /locus_tag="VIL39_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481635.1"
                     /translation="MNAHERHRTRTLWLAGVLHAFTHVYQVALMPLYLLIQRDFKFAS
                     VGQATLLLTVMMAACFGPSYPIGVLADRMNRNKLLGFGLALNGLGFVALALAPNYAWA
                     LVAVAVAGFGGSFYHPAATATVVRLFPDSAGKALGLVGIGAGVGFFVGPIYAGWRASL
                     LEPVLGPAAWRQPVLELGVLGIVVAALFAWLADAEQPAAASERKTVPAGKMFPTPALW
                     FFFLAYCLAFSVRDFAGASMGSLSSLFLQNAHNYDPKWTGLALSGIYVAATISNPLFG
                     YLSDGGRKRWITLALVVAAVMVALLPCVPPRWTIVVLVVYGFFFLATYPMTEAALMES
                     VPDAVRGRVFGIFVMGGGVIGNLSHWLVGAKVKQLGAAAYSVNAYLPIYAVIAFLMLV
                     SLAGLLCLPAIQRREAPVTHKATEEDSMPLLT"
     gene            complement(3731..>3918)
                     /locus_tag="VIL39_09175"
     CDS             complement(3731..>3918)
                     /locus_tag="VIL39_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481636.1"
                     /translation="PETGGEVSESETGLIRVFDLANVYSVMAIQTEDLGIRHRDGFTL
                     AGRAALAEPRGCSLMAV"
BASE COUNT          772 a         1318 c         1144 g          684 t
ORIGIN      
        1 tagccaatgc caaagagaaa gccaagcgca cggcctgcaa gagcaacatg cgccaggcca
       61 tcctttcggt tcacatgtat gcaaacgatt ggcgcgacta cgtgccggat gggcgtgaca
      121 acgacgatca atggcatgcc atccgcattc gcaacgatac ctacacgaac ctggttcagt
      181 acaccggaaa cctcaagatc atggactgcc ccaatttcac ttatggctct tttacgcgtt
      241 atagcgcgac ctggggttac ctgattggct acgcctatct gggccatgcc ctcgacaaga
      301 acaactcgag cttctggctg accacctcgc cgtactactg gcgctctcca ttgaagacaa
      361 ccgaatcccc caccaattat atcattgccg atgccaacac ctggggcggc ggtttgctga
      421 tggcgccgca ctgcaagacc gggccttacc agcgcaccag tcccggttca ccaaacgtag
      481 cggctacctt cattaacgac gccacgactg aaactcctca aagcgtcggc gcggttggcg
      541 gcaatgtcgg cctcctcgac ggctcggtct cgtggaagcg tatgaagcaa atgaagcagc
      601 agttcgcctc ctcctatgtt atctattggg gcaactggta gccattgagg gcgagcgtcc
      661 gcgcgagccc gatcttcggg aacggtttgg gctcgcgcgg acgctcaccc ttccgttgtc
      721 cagctaactt tgctccccgc gatacacgca cttcgcggtg caggtctcgg ccacagccac
      781 ttctgtcagt agcggcagtc tcggctttag tttcttccaa atccaaacgg caatgttctc
      841 gctggtcgga ttggcgaggc cggggacttc gttcaggtag tagtggtcga gcctttccca
      901 aatcggcttg aacgcgcggg agatatcggc gtaatccatc agccagccga gccgggggtc
      961 gcactcgccg gcgaccacta tctccacctt gaagctgtgg ccatgcagcc gccggcattt
     1021 gtgcgacctg ggcaagtgcg gcagcaaatg cgccgcctcg aactgaaagg ttttacgtag
     1081 ttccattctc acatttccca atccgtccac gttaccagat cgcccagcgc gggccggcca
     1141 tcgtgccgcc aggccaaggg tggctcttgc atctggccga gccggcacac ccgggtcacc
     1201 ccccaacgcg ccagctccgt cgccagcggc tgcgctttgt cttccggcgc ggcgaggcca
     1261 accgtggata cctggccccg aacactttcg gcgttctgca acgcctctgt catatccttc
     1321 acgcccttta tgtagatgaa ccggttgagg catgagagct gaaagcgcgg gtcggcctca
     1381 tagaccaccg tccaggcggt cgaattccgg ctgcaccaat gacgcgtgtc cggcgaatgg
     1441 gcggcgcgca cctcgtaaaa ggagcggcga gacgcgatgg ccgccgccga ttgcaccggc
     1501 aagtcacctc gcggctcgga ttcctcgcgc cgttccaatt cctccgccag catctcagcg
     1561 aattgctcgg gcggcacgcc gccgccgtgc tccacataaa tgacgtgcgg cgaaaggcag
     1621 ccctgttgat tccaggcggt cacatcggcc gccgcctgcc aggcaacctt cctcgcgtgc
     1681 agccccgtca acgctccgcc cgcaatgtag ccgaagctga cccggtggcc gtaaccgaga
     1741 aagcgggctt tcgccggcac ccgctggcga attgccgcca gcgtctcatc ggtgcccgtc
     1801 gccgtgacgc aatcggcctc gtcaaacagg gccttttcca ggtctgcgtt gcctccgcgc
     1861 cactcggcta cttcgaggca ggcgcccagt tttggttcca cttggtaaag cgagtgcgca
     1921 aagaggcggg gcagcaagga ggtgccgctg gcgcacttca caaactgcgc tgaccgcacc
     1981 agcacgccca gcaccatggc ggccagtgtg ggattcggca ggttgcccgc cgtgatctgc
     2041 gccagcagtt cgggagcgat ggctatggcg gcgcggcccg actttcgctc cgcttcgccg
     2101 gccaccatct catcgagccg gtgcccgtgg cctagatcct gttccaggag gacgtggaag
     2161 tgctcctgag tgagcagttt gaagaatgaa tccaagcccc gggccagtgt cgcgctggaa
     2221 aaaccggtcg ctgccggccc gcgttcgagc gccagcttgc ggaaggagta atccggctca
     2281 agccagctac gggccacgtc gctcagggtc ttcaccaggc tgtggctgga acggtgggcc
     2341 aagtagcgct cgcggttgcg cttgagcgtc tggcaggctt cgccaatcat cgcggcgctc
     2401 aatgtcgcct cgggcggcaa atcagccagg aagtaattcg gcaggttcat gttaacagcg
     2461 gcatcgaatc ctcttcggtc gctttgtgcg ttaccggtgc ttctcgccgt tgaatcgccg
     2521 gcaggcacag caggccggcg agcgacacca acatcaggaa agcgatcacc gcgtaaatcg
     2581 ggagataggc gttgaccgaa tacgcggccg cgccgagctg cttgactttc gcgccgacca
     2641 accagtggga aagattgccg atcaccccgc cccccatgac gaagatgccg aacacccgcc
     2701 cgcgcacggc gtccggcacc gattccatca gcgccgcctc ggtcatcgga taagtggcca
     2761 ggaagaaaaa gccgtaaacg accagcacca cgatcgtcca gcgcggcggc acgcacggga
     2821 gaagggccac cattaccgcg gccaccacca gggccagtgt gatccagcgc ttccggcccc
     2881 cgtcgctcaa gtaaccgaac agggggttgc tgatcgtggc ggcgacgtag attccgctca
     2941 atgccagtcc cgtccacttc gggtcgtagt tgtgggcgtt ctgcaagaac agcgagctga
     3001 gactgcccat gctcgccccg gcgaagtcgc gcacactaaa cgccaggcaa taggccagga
     3061 agaagaacca cagtgccggg gtggggaaca ttttccccgc cggaaccgtc ttccgttcgc
     3121 tggccgcagc gggctgctca gcatctgcca gccaggcgaa cagggcggcg accacaatcc
     3181 ccaggacgcc caactccagc accggctgcc gccaggcggc tggtcccaag accggttcga
     3241 gcaggctcgc gcgccagccc gcatagatcg gccccacaaa gaagcccaca ccggccccga
     3301 tgccgaccaa tcccagcgcc ttgcccgcgc tgtccggaaa caagcgcacc accgtggcgg
     3361 tggcggccgg gtgataaaaa ctgccgccga agccggccac cgccacggcc accagcgccc
     3421 aggcgtaatt cggcgccagc gccagcgcca cgaagcccag cccgttgagg gcgagcccaa
     3481 aaccgagcag cttgttgcga ttcatcctgt ccgccagcac gccgatgggg tagctcggcc
     3541 cgaaacaggc cgccatcatc accgtcagca gcagcgtcgc ctgacccacg ctggcgaact
     3601 tgaaatccct ctggatcagc aggtagagcg gcatcaacgc cacttggtac acatgtgtga
     3661 aggcatgcag cacgccggct aaccagagtg tccgggttcg atgtcgttcg tgcgcgttca
     3721 tcgaatcacg tcagactgcc atcagcgaac atccgcgcgg ctcggccagc gcagcccggc
     3781 ccgccagggt aaagccgtcg cggtggcgga tgcccagatc ttcggtttga attgccatca
     3841 ccgaatagac attggccaaa tcaaacaccc gaatcaaacc ggtttcgctc tcgctcactt
     3901 cgcccccggt ttccggcg
//
LOCUS       DATCGS010000444         3748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_137484, whole genome shotgun sequence.
ACCESSION   DATCGS010000444 DATCGS010000000
VERSION     DATCGS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137484"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1020)
                     /locus_tag="VIL39_09180"
     CDS             complement(121..1020)
                     /locus_tag="VIL39_09180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5481637.1"
                     /translation="MKRTMLWKISVTTAPEAEDAVTDWLAGAFGQPVSSYTDAETGIA
                     VVSVYVPTRPDWSRARQKELALGLRRIASSGLNLGRGQISLKSIRKEDWAESWKLHFK
                     PLLIGSALLLKPSWSRRRPRKGQAVVVLDPGLSFGTGRHPTTAFCLRQLVARRRSGRS
                     QSCLDIGTGSGILAIAAARLGYAPVDAFDFDPEAVRVALANARRNGVAAKIRFRRQDL
                     TKLPRHSTQQYSLVCANLVSSLLTAERERILARLRADGVLVIAGILEGEFAPVQRAFE
                     TAGLRLMAGRTQNEWRCGAFCWR"
     gene            complement(1034..1705)
                     /locus_tag="VIL39_09185"
     CDS             complement(1034..1705)
                     /locus_tag="VIL39_09185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5481638.1"
                     /translation="MKPSAPADPTSAPRPAAPSGTRLLLIRHAEVEASYQGVFGGRID
                     MDLSPRGHEQAAALAKCLYQKPLSAIYASPMKRVQQTLAPVLLNGAPKPVILPDLREV
                     DFGDWTGLAWEEVQAKFGISAFSWLDQLECDGIANAECAETLQDRVEPCLRQILAAHP
                     GQPVAVFCHGGIIRMLLAILLGWPLSRLGVFEIEYASLTQVLVLPEKAELQLLNFTPW
                     REVAS"
     gene            complement(1725..2426)
                     /locus_tag="VIL39_09190"
     CDS             complement(1725..2426)
                     /locus_tag="VIL39_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481639.1"
                     /translation="MTETTNHALVDVDGVEKVFRRGSEDIHVLKDLHLKVPSGEFLAL
                     MGPSGSGKSTLLNLIGGLDRPTRGTVSISGERVDELSDRKLAAWRARHIGFVFQLYNL
                     LPVLTAERNVELPLLLTHLSRVQRKRHVATALAIVGLSQRAKHYPRQLSGGEQQRVGI
                     ARAIVTDPTLLLCDEPTGDLDRKAGDEILDLLQGLNREHGKTIIMVTHDPHASARASR
                     TVYLNKGQLSAQPVD"
     gene            complement(2430..3656)
                     /locus_tag="VIL39_09195"
     CDS             complement(2430..3656)
                     /locus_tag="VIL39_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5481640.1"
                     /translation="MSSPKPTLDDLRIERSAEPESTSRFWPVAIGIVVLVLAVGVILW
                     RRQSGAVSVHTVLVREATGNGIERTVLNASGYVTARRQATVSSKVTGKVTEVLVEEGM
                     KVQEGQVLARLDDTNVKTSLRLAQAQLVSATNGLAETRVRIREADQELQRQTGLVKNK
                     IATQADYDHAEAALLALKARLEQQQSDVVVAEKQVAYWDQQLDDTIIRAPFSGIVTSK
                     NAQPGEMISPISAGGGFTRTGICTIVDMESLEIEIDVNESYINRVAAGQAVEATLDAY
                     PEWKIPSKVIAIIPTADRQKSTVKVRVGFDKLDPRILPEMSVKVAFREAGGSGPVAGR
                     TVIVPKAAVQQQEGRDVVFVMLSGKAERRAVTVSKTRSDEAVISAGVAAGERVIVDGP
                     AGLVDGAAVVEKRGNR"
BASE COUNT          664 a         1241 c         1135 g          708 t
ORIGIN      
        1 atgcgcccgg cgcactccgc caaggctttg tcctcccgtc actgatccat tgctttcgag
       61 gagttccgca gtagaggttg atgccgccct ggtcctctag gccggggggg ggcacaccat
      121 tcaccgccag caaaaagcac cgcagcgcca ttcgttttgt gtgcggccgg ccattagccg
      181 aagaccggcg gtttcgaagg ctctttgcac cggcgcaaat tccccttcca aaatgccggc
      241 gatgaccagc acgccgtccg cgcgcagtcg ggccaagatc cgttcccgtt cggccgtcag
      301 caggctggaa accaggttgg cgcaaaccag agagtattgc tgcgtgctgt ggcggggcag
      361 tttggtcaaa tcctgccgcc gaaagcgaat tttggcggcc accccgttcc gacgcgcatt
      421 ggccagggca acgcggacag cctccggatc aaaatcaaac gcgtccaccg gggcatagcc
      481 gagcctggcg gcggcgatgg ccagaatgcc ggacccggtg ccgatgtcca agcaagactg
      541 cgatctgccg gatcggcgcc gtgccaccag ttgccgcaga caaaaggccg tcgtcggatg
      601 ccggcccgtg ccaaagctca gcccgggatc gagtaccacc accgcctgcc ctttccgcgg
      661 gcgacgccgg ctccagctcg gcttgagcag cagcgccgaa ccgatcagca gcggcttaaa
      721 gtgcagcttc caggattccg cccaatcttc cttgcggatg ctctttagcg agatttggcc
      781 gcgaccgagg ttcagcccgc tgctggcaat ccgtcgaagg cccaacgcca attccttctg
      841 cctggcccga gaccagtctg gcctggtcgg aacatacacc gataccactg ctatcccggt
      901 ttcggcgtca gtatatgacg agaccggttg gccgaaggcg cctgcgagcc aatctgtgac
      961 ggcgtcttcc gcttccgggg cggtcgtcac ggatatcttc cagagcatgg ttcgtttcat
     1021 tgtttctcgc agcctacgac gcgacttccc gccagggggt gaagttgagc aactgcaact
     1081 cggctttctc cggcagcacc aggacttggg taaggcttgc gtattcgatt tcaaacaccc
     1141 ccagacgcga gagcggccag ccgagcagaa tcgccagcaa catgcggatt atgccgccgt
     1201 ggcagaaaac cgccaccggc tgaccgggat gggcagccag gatttgccgc aggcaaggct
     1261 ccactcggtc ctgcagggtt tcggcgcatt cggcattggc gatgccatcg cactccaatt
     1321 gatcgagcca gctaaaggcg ctgatcccaa actttgcctg tacttcctcc caggccagtc
     1381 cggtccagtc cccgaaatcc acctctcgca agtccggcag gatgaccggc ttcggtgcgc
     1441 cgttgagcag caccggcgcc agtgtttgct ggacgcgttt catcgggctg gcgtaaatcg
     1501 cgctcaacgg cttctggtac aggcactttg ccagggcggc agcttgctca tggccgcgcg
     1561 gcgaaaggtc catgtcaatc cggccgccga acacaccttg atagctcgct tcgacttccg
     1621 catggcggat gagcagcaag cgggtgccgg agggcgctgc gggacgaggc gcggaggtgg
     1681 ggtctgcagg ggcggacggc ttcacttggg tgcttggagg gcgattagtc cacaggctgg
     1741 gcgctaagct ggcccttgtt gagataaacc gtccgcgagg cgcgagcgga ggcgtgcggg
     1801 tcgtgcgtga ccatgatgat ggtcttgcca tgctcgcggt tcagcccctg gagcaagtcc
     1861 aggatctcgt cccccgcctt gcggtcgagg tcgccggtcg gctcgtcgca cagcagcaag
     1921 gtgggatcgg tgacaatcgc gcgggcgatg cccacgcgct gctgctcccc cccggaaagc
     1981 tggcgcggat aatgtttcgc ccgctgtgac aggcccacga tcgccaaagc agtcgccacg
     2041 tgccgcttcc gctgaactcg ggacaggtgc gtaagcagca gcggcaactc cacattgcgc
     2101 tccgccgtga gcaccgggag caggttgtag agctggaaaa cgaagccgat gtggcgcgcg
     2161 cgccacgcgg ccagcttgcg gtcggacaat tcatccacgc gctcgcccga gatgcttacc
     2221 gtgccgcggg tagggcggtc cagcccgccg atcaggttca gcaaggtgct cttgcccgaa
     2281 cccgatggcc ccatcagcgc caggaactcg cccgacggca cctttagatg caggtccttt
     2341 aggacgtgaa tgtcctctga cccgcggcga aagaccttct ctaccccatc cacgtccacc
     2401 agcgcatgat tggtcgtctc agtcatgcct caacggtttc ctcgcttttc gacgacggct
     2461 gcgccatcca ccagacctgc cggcccgtcc acaatgactc tctccccggc cgcgacgcca
     2521 gcgctgatca ccgcttcgtc agagcgcgtc ttgctcaccg tcacggcccg gcgctcggcc
     2581 ttgccgctca gcatgacgaa caccacgtcg cgtccttcct gttgctgaac ggcggctttg
     2641 ggcacgatca ctgtgcgccc cgccaccggc ccggacccgc ccgcctcgcg gaaggctacc
     2701 ttcacactca tttccggcag gatgcgcgga tcgagcttgt cgaacccgac gcgcaccttg
     2761 acggtggact tctgccggtc ggcggtcggg atgatcgcaa tcactttcga gggaatcttc
     2821 cactcgggat acgcgtcgag cgtcgcctcg accgcctggc ccgctgcaac gcggttgatg
     2881 tagctttcat tgacatcaat ctcaatctcc agggattcca tgtccacgat ggtgcaaatg
     2941 cccgtgcgcg tgaagccgcc cccggcggat ataggtgaaa tcatctcgcc cggctgcgcg
     3001 ttcttcgagg tgacaatgcc ggagaaaggc gcgcggatga tggtgtcgtc caactgctgg
     3061 tcccaatagg ccacctgctt ttcggcgacc accacatcgg attgctgttg ctccagcctc
     3121 gctttcaggg ccagcagcgc ggcctcggcg tgatcatagt cggcctgcgt ggcgatcttg
     3181 ttcttcacca ggccggtctg gcgctgcaat tcctggtccg cctccctgat gcgcacgcgt
     3241 gtctcggcga gcccattcgt tgcggacacc aactgggctt gtgccaggcg caagctggtc
     3301 ttcacattcg tatcgtcgag gcgcgcgagg acttgccctt cctgcacttt catcccttcc
     3361 tccacgagca cctcggtaac cttgccggta accttggacg aaacggtggc ctgccgtcgg
     3421 gcggtgacat agcccgaggc attcaagacc gtgcgttcga tgccgttgcc ggtggcctcg
     3481 cgcaccagca cggtgtgcac cgagacagct cccgactgcc tccgccacaa gatcacaccc
     3541 actgccagca ccaaaacgac gatgccaatc gccaccggcc agaatcggga cgttgactcc
     3601 ggttcagcgc ttcgctcgat gcgcagatca tctagcgtag gtttgggcga actcatagat
     3661 aaagaccgcg cacagggaac cgctttttcg cctcagggtc aattatggag tgccggacgg
     3721 cgcggcgctg aggtgcgggc cgtgaagt
//
LOCUS       DATCGS010000445         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_137545, whole genome shotgun sequence.
ACCESSION   DATCGS010000445 DATCGS010000000
VERSION     DATCGS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137545"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="VIL39_09200"
     CDS             <1..1854
                     /locus_tag="VIL39_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY5481641.1"
                     /translation="PTQTTAALILELSQDPTVALVEPNHLRWATDMRTPNGPLFGQLW
                     GLQNTGQAVNAVTGTAHADIGFLSAWGLARPSTNEVVVAVIDSGLDLTHPDIVSNLWT
                     NPGEISNNGLDDDHNGYVDDVHGYDFVNGTGAVTDSGFHGTHVAGTIAASGNNGLGVI
                     GVDFQAHIMALKASNDGTTMTSAAIIEAMQYAAMMKTRGVNVVAINASFGGSSYNSTE
                     SSTIQAAGNVGIVFCAAAGNNTNNNDTVAYYPASYRLSNMIVIAATDQNDTLATFSDY
                     GATTVDLAAPGVNILSCLPVAQFGNTSYVQQASAVYQANPLTYSGLTTSNGITAAIYY
                     CGLGNPADFPAAMRNNIALIQRGTLYFSDKVSNATAAGARAAVIFNNTNGNFLGTLGT
                     AGNWIPAIALSQADGQALLATLPATGKVVNAPDPANIYQLLDGTSMATPHVAGAVAFA
                     AMNFLTETVAQRIQRILANVTHVAGLAGKVVTGGRLNLARSVDTDGNGLPDWWEQEFF
                     GRLTGTDPNADPDHDRASNLAEFLAGTNPTNFNSALRLTALRVAGTNGVVLEWPGVSG
                     RYYRLLRATNLLNGFNTLVRTNLPATPPLNTVTDAAPASAAPRFYRLELEQ"
     gene            complement(1903..2946)
                     /locus_tag="VIL39_09205"
     CDS             complement(1903..2946)
                     /locus_tag="VIL39_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEY5481642.1"
                     /translation="MKLARRTKPCPPVPALLTEVRELILSARQTVARGVNAALVMLYW
                     KIGERIRRDILLEKRAGYGKEILQILSAKLVLEFGRGFSQRNLDSMAQFVEAFPDSKI
                     VATLSRELGWSHFVQLIPLKKHLQRDFYAEMCRLERWSVRALRQKISGMLYERTALSK
                     KPDEVAALELKGLREADKLTPDLVFRDPYFLDFLGLKGAYSEKDLEGAILRELESFLI
                     ELGGDWAFLARQKRMTVDGEDFYLDLLFYHRRLRRLIALDLKLGKFAPADLGQMEFYL
                     RWLKKHEMRPGEEEPLGLILCAEKSDERVELLELEKRGIRVASYLTELPPRQMLEQKL
                     HDAVRLARARLCR"
     gene            complement(3030..>4613)
                     /locus_tag="VIL39_09210"
     CDS             complement(3030..>4613)
                     /locus_tag="VIL39_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY5481643.1"
                     /translation="REFYTFSEAFKTEIGSISVPTLEAVCAMMPAKDWEVINDDWAEH
                     DFCSGAQQGDRYPYIITDRYGPIASLPDFVRKSQLANYECFRAMYEGRFAKLFHPETG
                     VITWMSSPAQPSFVWQLYSHDLEPNASLYGTRKACEPVHIMLNQKDWHVMVVNNTPEP
                     LKGLTAKTTVYNLDGSAQYTHSDTATAAPSAATDLGAIGFPSALSAVHFLKLELRDGK
                     GRLLSDNFYWRAAPGDEDNFQALNTLPTVALDIQASRHDAGGKCLLEAVLRNPSQTIA
                     LMSHLQLRKARSGQRVLPVFYSDNYVSLLPGERKTLTVEAAASDLDGDAPLLAVDGWN
                     VTVKPTPASGSRAVVVAPNTEAQVSATAAVPASAAGELLGINCGGSQIGFFRFGAPPP
                     AFAQDYNYQGGKTDSVPDTGKAIDTQVPNAAPAAVYQSERWGKCTYTIPAKPGRPCMV
                     RLHFAEVKLEPGQRKFNVEINGRRVLADYDIAAEAGKGKAVVKDFPAISPDGSGNIVI
                     AFTRGSADEPKICGIQILK"
BASE COUNT          878 a         1509 c         1359 g          867 t
ORIGIN      
        1 ccgacgcaaa ccaccgccgc gctgatcctg gagttgagcc aggatcccac ggtggctttg
       61 gtcgaaccaa accacctgcg ctgggccacg gacatgcgga caccgaacgg cccgttgttc
      121 ggccagttgt ggggcctgca aaacaccggc caagctgtca acgccgtcac cggcacagcg
      181 cacgcggaca ttggttttct gagcgcctgg ggcctggccc ggccgtccac caacgaggtg
      241 gtggtggcgg tcattgacag cggcttggat ctcacgcacc cggacatcgt gagcaacctg
      301 tggacgaatc ctggtgagat ttcgaacaac gggctggacg acgatcacaa cggttacgtg
      361 gacgacgtgc acggttacga ctttgttaac gggaccggcg cggtgaccga ttccgggttt
      421 cacggcacac acgtcgccgg gaccatcgcg gcttcgggca acaatggctt gggcgtgatc
      481 ggtgtggatt tccaggccca catcatggcg ctcaaggcgt ccaacgacgg aaccacgatg
      541 acctccgctg ccatcatcga agcgatgcaa tacgccgcca tgatgaagac ccgcggcgta
      601 aacgtcgtcg ccatcaacgc ctccttcggc ggcagtagct acaacagcac agaatcctct
      661 accatccagg ccgccggcaa cgtcggcatc gttttctgcg ccgccgccgg caacaacacg
      721 aacaacaacg acacggtagc ctattacccc gctagctacc gcctgtcgaa catgattgtc
      781 attgccgcca ccgaccaaaa cgacacgctg gccacctttt ccgattacgg cgcgacgacc
      841 gttgacctcg ccgcgccggg cgtcaacatc ctctcctgcc tgccggtggc gcagttcggc
      901 aacacctcct acgtccagca agcctcggcg gtttatcagg ccaacccgct gacctattcg
      961 ggactcacca cttccaatgg tatcaccgcg gcgatctatt actgcgggct gggtaatccg
     1021 gcggatttcc ccgccgccat gcgcaacaac atcgcgctga tccagcgcgg cacgttgtat
     1081 ttctcggata aggtttccaa cgccaccgcg gcgggcgcgc gggcggcggt gattttcaac
     1141 aacaccaacg gcaatttcct cggcaccctt gggaccgccg gcaactggat tcccgcgatt
     1201 gcgctctccc aggccgacgg ccaggcgctg ctcgcgacgc tgccggccac tggcaaggtg
     1261 gtcaatgcgc ccgatccggc caacatctac cagttactcg acggcacctc gatggccacg
     1321 cctcacgtcg ccggcgcagt ggcctttgcc gcgatgaact ttctcacgga gaccgtcgcg
     1381 caacgtattc aacgcatcct tgccaatgtt acgcacgtcg ccggcctggc cggtaaagtc
     1441 gtgaccggcg gacgcctcaa tctggcccgt tccgtggaca ccgacgggaa cggcctgcct
     1501 gattggtggg agcaagaatt ctttggccgc ctgaccggca ccgatcccaa tgccgatccc
     1561 gaccacgatc gcgcgagcaa cctcgccgag tttctcgcag gcacgaaccc gaccaacttc
     1621 aactccgccc tccggctcac cgcgctgcgc gtcgcgggca cgaacggtgt cgtgttggaa
     1681 tggccgggcg tgtcgggccg ctactaccgg ttgctgcgcg cgacgaatct gctcaacgga
     1741 ttcaacaccc tcgtccgcac gaacctccct gccactccgc cgctcaacac ggtgaccgat
     1801 gccgcaccgg ccagcgcagc cccgcgattc taccggctcg agttggaaca gtgaccgatg
     1861 ccacgcgctc acgagagaat ctgaatgccg caaaccttgg gctcatcggc aaagccgcgc
     1921 tcgggccagg cgcacggcgt cgtggagttt ttgttccagc atctggcggg gcggcagttc
     1981 ggtcagatac gacgccaccc ggatgccgcg cttttccaac tccagcagct ccacgcgctc
     2041 atcggatttc tcggcgcaga gaatcagccc cagcggctcc tcttcccccg gccgcatctc
     2101 atgcttcttc agccagcgca gataaaactc catctgtcca aggtccgccg gggcgaactt
     2161 ccccagcttc aagtccagcg caatcagccg ccgcagccgg cggtgataaa acaacaagtc
     2221 caggtagaaa tcctcgccat ccaccgtcat gcgcttctgc cgggccagga acgcccagtc
     2281 gccgcccaac tcgatcagga acgactccag ttctcggagg atggcccctt ccaggtcctt
     2341 ctctgaatac gctcccttga ggccgaggaa atcgaggaag tagggatcgc ggaagaccag
     2401 gtcgggcgtg agcttgtccg cctcgcgcaa gcccttcagt tccaacgcgg cgacttcatc
     2461 cggcttcttg gatagtgcgg tgcgttcgta gagcatcccg ctgatcttct ggcgcaaggc
     2521 gcgaacactc cagcgctcca agcggcacat ttcagcatag aagtcccgct gaaggtgctt
     2581 cttgagcggg atgagttgga caaagtggga ccagcctaat tctctcgaca gtgtcgcaac
     2641 tatctttgag tcggggaaag cttcaacaaa ttgcgccatg gaatcaagat tgcgctggga
     2701 gaaaccgcgg ccaaactcca ataccaattt tgcagacagt atctgcaaaa tctccttccc
     2761 gtaaccagcc cgcttttcca gcaggatatc ccggcggatg cgctcaccga tcttccaata
     2821 caacatcacg agggctgcgt tcaccccgcg ggccaccgtc tgccgcgccg acagaatcaa
     2881 ctcgcgcacc tccgtaagca gggccgggac gggtgggcac ggcttggtgc gccgtgccaa
     2941 cttcatattg cccgacatct ttgcgctcat agccatgtag ctttgcagtt tcccttttcg
     3001 ccgcagccag ctcaagctta tcggccctct cacttaagaa tctgaatacc gcaaatctta
     3061 ggctcatcag ccgagccgcg cgtgaaggcg atgacgatgt tgccgctgcc atcgggtgaa
     3121 atggctggga agtccttcac gaccgctttg cctttgccgg cttcggcggc gatgtcgtag
     3181 tcggccagca cacggcggcc attgatctcc acgttgaact tgcgctggcc cggctcaagc
     3241 ttgacctcgg cgaagtgcag gcgaaccatg cagggccgcc ctggcttggc gggaatggtg
     3301 taagtgcatt tcccccatcg ctcgctctga tagacggcgg cgggcgccgc gttggggacc
     3361 tgggtgtcaa tggctttgcc ggtatcaggc actgagtcgg tcttgccgcc ctggtagttg
     3421 tagtcctgag caaacgcggg tggcggagcg ccgaagcgga agaagccgat ctgactgccg
     3481 ccgcagttga tgcccagaag ctcacccgct gcactggcgg gcaccgctgc ggtcgcgctg
     3541 acttgggcct cggtatttgg cgccaccacg acggctcggc tgccagaggc aggcgtgggc
     3601 ttcacggtca cattccagcc atccacggcc agcaggggcg cgtcgccgtc caggtcactg
     3661 gcggctgctt cgaccgtgag cgttttacgc tcgcccggca gcagcgacac atagttgtcg
     3721 ctatagaata ccggcagcac gcgctgaccg gagcgtgcct tccgcaactg cagatggctc
     3781 atcagagcga tggtctggga ggggttgcgc agcactgcct ccaggaggca cttgccaccg
     3841 gcgtcatgcc gcgaagcctg gatgtcgagc gcgaccgtgg gcagcgtgtt cagcgcctgg
     3901 aaattatcct catcgccggg ggcggcgcgc cagtagaagt tgtccgaaag cagccggccc
     3961 ttcccgtccc gcagttcgag tttgaggaaa tggacagccg acaacgcgct aggaaagccg
     4021 atagcgccga ggtccgtggc ggcgctcggg gcggcggtgg cggtatcgct atgggtgtat
     4081 tgggcgctgc catcaaggtt gtaaacggtc gtcttggccg tcagcccctt caacggttcg
     4141 ggcgtgttgt tgacgaccat gacgtgccag tccttctgat tcagcatgat atggaccggc
     4201 tcgcaggcct tgcgcgtccc atacaacgag gcgttcggct ccaggtcatg gctgtagagt
     4261 tgccagacga agctcggctg ggccgggctg ctcatccagg tgatgacgcc ggtttccggg
     4321 tggaacagct tggcaaagcg gccttcgtac atcgcccgga aacactcata gttggcgagt
     4381 tgggatttgc gcacaaagtc cggcaggctg gcaatcgggc cgtagcggtc ggtgatgata
     4441 tagggatagc ggtcgccctg ttgcgcgccg ctgcagaaat catgctcggc ccagtcgtcg
     4501 ttgatgacct cccaatcctt cgccggcatc atcgcgcaga ccgcttcgag cgtcggcacg
     4561 gagatagacc caatctccgt cttaaaggcc tcgctaaatg tgtagaactc ccg
//
LOCUS       DATCGS010000446         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_533776, whole genome shotgun sequence.
ACCESSION   DATCGS010000446 DATCGS010000000
VERSION     DATCGS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533776"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VIL39_09215"
     CDS             complement(<1..749)
                     /locus_tag="VIL39_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY5481644.1"
                     /translation="MNNQILRLAALLVYFTITVAFAADSAPRRVENFNRDWKFARGAQ
                     AGAEAMDFNDAAWLPVRLPHDWAISGPYEPQGDANTGKLPWRGEGWYRKSFALPAADA
                     GQRVYLDFDGVMAMPTVYVNGQKAGGWDYGYMSFRVDATDFVKAGQRNVVVVHVDTRQ
                     HNSRWYPGAGIYRKVQLVVNAPVHVAQWGTFVTTPQVASDSATIHVETTLENHTLSAV
                     EPEVETTLLDPKGKEVAKLRMSFAVPANGSQP"
     gene            970..2655
                     /locus_tag="VIL39_09220"
     CDS             970..2655
                     /locus_tag="VIL39_09220"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY5481645.1"
                     /translation="MNPKPLSAIEISRKTKLQPIEVVAAKVGIPEEDLEFFGKTKAKV
                     TLDLYRRLADAPTGKLILVTAINPTPAGEGKTTTSIGLTDALNRLGRRAIVCLREPSV
                     GPCFGVKGGGTGGGYAQVAPMEEINLHFNGDFHVVTSAHNLLAAMLDNHLHFGNELGI
                     DQRRIVWRRVLDMNERALRQVVIGLGGTGNSIPRESGFDITPASEIMATLCFSESLPE
                     LKERLGRTIVAYSQTGKFITARDLKADGAMTVVMRYGLQPNLVQTLEGNPALVHGGPF
                     ANIAHGCNTVTATKLGLRLAEFTVTEAGFGADLGAEKFLNIKCRKAGLRPDAAVLVAT
                     IRALKYHGGIKVPDLPKPNLAAVTAGLDNLLKHVENLQSFGLPVIVSINVFAGDTPDE
                     WKVVSDACAKVGVPVAFSEHFARGGAGAEDLARLVLETTSQPAGGPIKFTYPDNLPLL
                     EKVRCIARKIYGADDVVTEGRTAEKLKSFEDAGFGHLPICMAKTQYSFSADPNRRGRP
                     RGFSVPLRDARLSAGPGFVVVLTGDIMTMPGLPKVPAAESMDVDANGNTVGLF"
BASE COUNT          517 a          977 c          876 g          506 t
ORIGIN      
        1 ggctgcgagc cgttggccgg cacggcgaaa ctcatccgca acttcgcaac ctctttgccc
       61 ttcggatcga gcagcgtggt ttccacttcc ggttcgaccg ccgagagggt gtggttttcc
      121 agcgtggtct ctacatgaat cgtggccgag tcgctggcaa cctgaggcgt cgtcacgaag
      181 gtcccccact gcgcgacatg cacgggcgcg ttcacgacga gctgcacctt gcgatagatg
      241 cccgcgcccg gataccagcg cgagttgtgc tgacgcgtgt ccacgtggac gaccacgacg
      301 ttgcgctgcc cagccttcac gaaatccgtc gcgtccacgc ggaacgacat gtagccgtaa
      361 tcccagccgc cagccttctg gccgttgaca tatacggtcg gcatcgccat cacgccgtca
      421 aagtcgagat acacgcgctg gcccgcatcg gcggcgggca gggcgaacga cttccgatac
      481 cagccttcgc cgcgccacgg gagcttgccg gtgttcgcat caccctgcgg ctcgtaggga
      541 ccgctaatgg cccaatcatg cggcaggcgc accggcagcc aggccgcgtc attgaagtcc
      601 atcgcctcgg ccccggcttg cgcccccctg gcgaacttcc agtcgcgatt aaagttctca
      661 acccggcgcg gggccgaatc ggctgcgaag gcaactgtga tcgtgaagta aaccagcaag
      721 gctgccaggc gtaaaatttg gttgttcatg ctatcgggcg acgagtgtgc agaccgtgaa
      781 ggtgtcggtc aagtatctca gaacgaggcg cggcaacctc ttctagctga cgctcgcgcc
      841 gcaagcggca gactggtatt gacgagccgc accggctccg cataatgagc gcggtcaata
      901 gattgtcgag gctggagcat ttgccggcgt caaacgcatg aagtagcgtt gctcgcaccc
      961 gatcagaata tgaatcccaa gccactcagt gctatagaga tatcccgcaa gaccaaattg
     1021 cagcccatcg aggtcgtcgc ggccaaggtg ggcatcccgg aagaggatct tgagttcttt
     1081 ggcaaaacca aggccaaggt caccctcgac ctctaccggc ggctcgccga tgcccccacc
     1141 gggaagctga ttctcgtcac cgccattaac cccaccccgg ccggcgaagg caaaaccacc
     1201 accagcatcg gcctgaccga tgccctcaac cggctcggca ggcgggctat cgtctgcctg
     1261 cgcgaaccgt ccgtcggccc ctgcttcggc gtcaagggcg ggggcaccgg cggcggctat
     1321 gcccaggttg ccccgatgga ggagatcaac ctgcatttca acggcgactt ccatgtggtc
     1381 acctcggccc acaatctcct ggcggccatg ctcgacaacc acctgcattt cggcaacgag
     1441 ctgggcatcg accagcgccg catcgtctgg cgccgcgtgc tcgacatgaa cgaacgcgcg
     1501 ctccggcagg tggtgatcgg actgggcggc accggcaact ccattccccg cgagtccggt
     1561 ttcgacatta ccccagcctc cgaaatcatg gcgacgctct gcttttccga atccctgccg
     1621 gaactcaagg aacgactggg ccggactatc gtcgcctact cgcaaaccgg caaattcatc
     1681 accgcccgcg acctcaaggc cgacggcgcg atgaccgtgg tgatgcgcta cggcctccaa
     1741 cccaacctcg tccagaccct cgaaggcaac ccggccctgg tccacggtgg ccccttcgcc
     1801 aacatcgccc acggctgcaa taccgtcacc gccaccaagc tgggcctgcg cctggccgaa
     1861 ttcaccgtca ccgaggccgg ctttggcgcg gacctcggcg cggagaaatt cctgaacatc
     1921 aagtgccgca aagctggcct gcgccccgac gccgcggtgc tcgtcgccac catcagagcc
     1981 ttgaaatacc acggtggcat caaggtgccg gaccttccca aacccaacct ggccgcggtg
     2041 accgccgggc tggacaacct gctcaagcat gtcgagaacc tgcagtcctt cggcctgccg
     2101 gtcattgtct cgatcaatgt ctttgcgggc gacaccccgg acgaatggaa ggtggtcagc
     2161 gacgcctgcg ccaaagtcgg cgtgccggtc gcgttttcag agcatttcgc gcggggcggc
     2221 gccggcgccg aagacctggc gcgcctcgtc ctcgagacca cctcccagcc cgctggcggt
     2281 ccgatcaagt tcacctatcc ggacaacctg ccgctgctgg aaaaagtgcg ctgcatcgcc
     2341 cgcaagatct atggcgccga cgatgtcgtg acggagggcc ggacggcgga gaagctcaag
     2401 tcgttcgagg atgccgggtt tggccatctg cccatctgca tggccaagac ccaatactca
     2461 ttttcagccg acccgaaccg gcggggccgc cctcgcgggt tcagcgtccc gctgcgcgac
     2521 gcgcgactca gcgccggccc gggattcgtg gtggtgctga cgggcgacat catgaccatg
     2581 cccggcctgc ccaaagtgcc tgcggccgag tccatggatg tggatgccaa cggcaacacc
     2641 gtcggcctgt tctaatgcct tcccggccga accggtggct cgtggccacc atgggcaccg
     2701 cgctgctggc cgtcggcttc tcgctctcgc tgctgctgcg tcagccagcg cgtgcagctc
     2761 ggcctgaggc tcatgggtag ccttggggta gccttggggt agccttgggg tagccttggg
     2821 gtggccttgg ggtggccttg gggtggcctt ggggtggcct tggggtagcc ttggtg
//
LOCUS       DATCGS010000447         3766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_233190, whole genome shotgun sequence.
ACCESSION   DATCGS010000447 DATCGS010000000
VERSION     DATCGS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233190"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2447)
                     /locus_tag="VIL39_09225"
     CDS             complement(<1..2447)
                     /locus_tag="VIL39_09225"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY5481646.1"
                     /translation="MMRRSCSLVLLVSLALAVPGFAARGASLKVEGLKCEYRVNPLGL
                     DTPQPRLSWLLESPQRGQRQTAYQVLAASTPDLLAKGKGDLWDSGKVASGESVQVGYG
                     GQPLRPGQRVYWKVRSWDREGQPSGYSPAAWWEMGLLSPADWRAAWITRKRAEPLSEG
                     QMFEDHPAPLFRKGFLIEKKISRARAYVSGLGYYELRLNGQRVGDQVLDPGWTAYAKR
                     VLYSSYDVTDQLKRGQNALGVMLGNGWFDPLPLRLWGHINPRDNLTIGNPRVIVQLVV
                     DFTDGTSQTVVTDESWKVGNGPILRNSVYLGEVYDARKEQPGWDRPGFDDSGWPQAVS
                     ATEPLGSLRAQSAPPIRVTRTLKPIKLTEPKPGVWIFDLGQNFAGWARLRVQGPAGTR
                     VRLRYGELLYPDGTLNGMTAVCGQIKQGGPNYRYDGVGAPKTAWQMEEYILKGQGEEV
                     YTPRFTFHGFRYVEVTGFPGKPTLNTLEGLRLNSDVAPVGSFTCSNERFNRIQQMVLW
                     TELSNLLSVESDCPHREKFGYGGDIVAAGEMAMLNFDMARFYGKAVDDLADAVRPNGG
                     FTETAPFVGISDEGLGERSGPVGWGTAQPFLLWRLYQYYGDRRLIEENYELTKRWIAL
                     LQSRAKDGILDNGISDHESLAPKPRALTGTGFYYFNVKLFANLARVLGKNAEAAEAEA
                     LAATIKTAFNRKFLQPGTGRYDAATQACQAFALYLGLVPVEEQERALAVLVRDIEDAH
                     AGHLTTGIFGTKFMLHALADSGRADVAFKVVNQKTFPGWGYMLENGATTLWEHWEFSD
                     NTFSHNHPMFGSVSEWF"
     gene            complement(2490..3047)
                     /locus_tag="VIL39_09230"
     CDS             complement(2490..3047)
                     /locus_tag="VIL39_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5481647.1"
                     /translation="MVLLTGLAVFGGQMAKAEPLKPDILFLMPDEMRGDCLSVLGHPA
                     VRTPQLDEQIVPLIAEFKERSRKARRPWVILFTADHGEMLGDHGFFRKCEPFEGSANI
                     PFLVAASPELGFKAGMRSLQPVCLEEVMPTLLELAGARCPKPMDGVSLVPTLRGSKQV
                     VRECLHFEHAPCYSQKQAFHALTAE"
BASE COUNT          724 a         1253 c         1110 g          679 t
ORIGIN      
        1 taaaaccact cgctgaccga gccgaacatc ggatggttgt gcgagaaggt gttgtcgctg
       61 aactcccaat gctcccacaa agtggtcgcg ccgttttcca gcatatagcc ccagcccggg
      121 aacgtctttt gattgactac cttgaacgcc acatccgcgc ggcccgaatc cgccagcgcg
      181 tgcagcatga acttggttcc gaaaatgccc gtggtcagat ggcccgcgtg cgcatcctca
      241 atgtcccgca ccaggaccgc gagcgcgcgc tcctgttcct caacgggcac caggcccaaa
      301 tacaaggcga acgcctggca agcctgcgtg gccgcatcgt agcggccggt gcccggctgg
      361 agaaacttcc ggttgaaggc cgtcttgatg gtcgcggcta gcgcctccgc ttccgccgcc
      421 tcggcgttct tccccagcac ccgggcgagg ttggcaaaca gcttcacgtt gaaatagtag
      481 aaacccgtgc cggtcagagc gcgcggcttc ggcgcgaggc tctcgtgatc actgatgccg
      541 ttgtccagga tgccatcctt cgcgcgcgat tgcagcagcg cgatccaacg cttcgtcagc
      601 tcgtagttct cctcgatcag ccgccggtca ccgtaatact gatagagccg ccacaagagg
      661 aacggctgtg ccgtgcccca gcccaccggc cccgacctct ctcccaggcc ctcgtcactg
      721 atgcccacga agggcgcggt ctccgtgaac ccgccgttcg gacgcaccgc gtccgccagg
      781 tcatccacgg ccttaccata gaagcgcgcc atgtcgaagt taagcatcgc catctcgccg
      841 gcagcaacga tgtcgccccc atagccgaac ttctcgcggt gcgggcagtc ggattccacg
      901 ctcaacaggt tgctcaactc cgtccacagc accatctgct gaatccggtt gaaccgctcg
      961 ttcgagcagg tgaatgagcc cacgggcgca acgtccgagt tgagccgcag cccttcgagt
     1021 gtgttgagcg tcggcttgcc cggaaagccg gtgacctcca catagcggaa gccgtggaag
     1081 gtgaaacgcg gcgtatagac ctcttcgccc tgccccttga ggatgtattc ctccatttgc
     1141 caggccgtct tcggcgcgcc gactccgtca tagcggtagt tgggaccgcc ttgttttatc
     1201 tggccgcaga ccgcggtcat gccgttcagt gtgccgtccg gatagagcag ttccccatag
     1261 cgcaaccgca cgcgcgtgcc ggcggggccc tgaacccgca gccgagccca gccggcgaag
     1321 ttctgcccca ggtcaaagat ccaaacgcct ggctttggct ccgtgagctt gatcggtttc
     1381 aatgtccggg ttacccgaat cggcggcgca ctttgcgccc gcagggaacc gagcggctca
     1441 gtggcgctta ccgcctgcgg ccagcccgaa tcgtcgaacc ccggccgatc ccaacccggc
     1501 tgctccttgc gcgcgtcata gacttcgccc agatacacgc tgttccgcag gatgggtcca
     1561 ttgcccacct tccagctctc gtcggtgaca accgtctgtg aagtgccgtc agtgaaatca
     1621 accacaagct gcacgatgac ccgcggattc ccgatcgtga ggttgtcgcg cggattgata
     1681 tgcccccaca agcgcaaggg cagcggatcg aaccacccgt tgccgagcat cacccccagc
     1741 gcgttctgcc cccgcttcaa ctggtcggtc acatcataac tggagtagag cacgcgtttc
     1801 gcataggcag tccaaccggg gtcgagcacc tggtccccga cacgctggcc gtttaagcgc
     1861 aactcgtaat agcccagccc gctcacatag gcccgggcgc ggctgatctt cttctcgatc
     1921 aaaaacccct ttcggaatag gggcgccggg tgatcttcga acatctgccc ttccgagagc
     1981 ggctcggccc gcttacgggt gatccaagcg gcgcgccaat ccgctggcga cagcaatccc
     2041 atttcccacc atgctgccgg gctgtaaccc gagggctggc cctcgcggtc ccaggagcgc
     2101 actttccaat agacccgctg acccgggcgc aacggctgcc cgccgtagcc tacctggacc
     2161 gattcgcccg aggcgacttt accgctgtcc cacaaatcac ctttgccttt ggccagcagg
     2221 tcgggggttg aggccgcgag cacttggtaa gccgtctgcc tctggccgcg ctgcggggat
     2281 tccagcagcc agcttagccg cggctggggc gtatcgagtc ccagcgggtt gacgcggtat
     2341 tcgcatttca aaccctccac cttcaggctg gcaccccggg ccgcgaaccc cgggaccgca
     2401 agggccagtg aaaccaatag aaccagtgag cagcttcgtc gcatcataag agggtgcatt
     2461 cgtcagtaag ttgtaacggg tggatcattc tattcggcag tgagggcgtg gaaagcctgc
     2521 ttctggctat aacacggcgc gtgctcgaag tgcaggcact cgcggaccac ctgcttactc
     2581 ccgcgaagcg tgggcaccaa gctcaccccg tccatcggtt tggggcagcg tgcgcccgcc
     2641 agctccagca aggtgggcat cacctcttcc aggcaaaccg gctgcaagga gcgcatgccc
     2701 gccttaaacc ccaactccgg cgaagcggcg accaggaaag ggatattggc cgaaccctcg
     2761 aacggctcgc acttccggaa gaaaccgtgg tcgccgagca tctcgccatg gtccgccgta
     2821 aataggatga cccagggccg cctggctttc cggctgcgct ccttgaactc ggcgatgagc
     2881 ggcacgatct gctcgtccaa ctgcggcgtg cgcaccgccg gatgcccgag caccgacagg
     2941 caatcgccgc gcatctcgtc gggcatgagg aacagaatat cgggcttgag cggctccgcc
     3001 ttggccatct gcccgccgaa aacagcaagg ccggttaaca ggaccaacca cggccttatg
     3061 cgagggcaca atcggacatt cacgtatcgc ttcacgcgcg ctcaaagtag ccagtcgcct
     3121 tctgtgccgt caggctaaat ctttcgcagt taggcagcgg gcgccgccaa acgcggttac
     3181 gccgttcaac tggctttgag atggctttgg ggtggctccc ctccgttaag ccgcagagac
     3241 gcgatggacg cagcgcggcc aaggtaccgc cggattgtgc gcgaagcgtt tgcgtccagc
     3301 ttgctggcgc tttggtaagg tgtgggatgg tcagaaagcg ggagcaagct ccgcgcactc
     3361 caaacgcttc gcgtggcagt ccatccagaa agacccaagc aggctgcgaa tagagcgcga
     3421 gcagtgtcgg agcgcggata ttcgtctctg ccaaccggcg tgaaaggaac tggcggacaa
     3481 aaacgtccgc gctcctacaa tcccaagggg attggacgtt tccgaaacgc actgaggcta
     3541 agccaaggcc ggggctcgaa aggtccgttg cggcctccaa aagctccgtc tgtcagccaa
     3601 acgcctgtca caaaaccgtg ggcggacagc gagattccag taacgcgaag tcagcggcgg
     3661 aagccaacca ccgatgacgt tcgatttgtc tctgggtgag tcggctggct cccgtcgggt
     3721 gcaccggctg gcgcggcccc gaggcgatgg aataaccact tcaggt
//
LOCUS       DATCGS010000448         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_582857, whole genome shotgun sequence.
ACCESSION   DATCGS010000448 DATCGS010000000
VERSION     DATCGS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582857"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VIL39_09235"
     CDS             complement(<1..621)
                     /locus_tag="VIL39_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481648.1"
                     /translation="MNRDERQPASGMNTSSTSLDPQPPTLNPQRPRLTRREFLASSAL
                     LALAAAMPGCKTTEDGLGYEPIIDIHQHVGYSGRSDEVLFAHQRAMGVTKTILLPAGR
                     SANSASTHNGVSNGLQAQCLGNQFCYRFAKAHPKHYLFAACEVPDLPDAVQEIEKYLK
                     LGGVAIAELKFGVECDSPEMQKLYQVAADYRVPVLMHWEHKMYNYGF"
     gene            706..>2014
                     /locus_tag="VIL39_09240"
     CDS             706..>2014
                     /locus_tag="VIL39_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEY5481649.1"
                     /translation="MSNLRRKGGEGMLTGSAVVAVTACFFLSCTGWSAEPKPESNVVP
                     YVASVPATNALAAFRVRPGFRLELVAREPAVISPVAMAFDENNRLFVVERREDSSAPG
                     TNAHSGRIHLLEDTEGDGEFHLSTVYADNLPWASAVACCSGGVFVATSPDIIFLKDTK
                     TNGIADVRKAIFTSFTGTNLLGARALPNNFNWGMDNRIHAASAGIAAFVPESGAPGAP
                     LVSLTGADFSFDPRALAMCAEAGPAESGLSFDDWGRKFTCDPLRPLRSPRYEPRYLAR
                     NPFVPPPPQMIEVASPATPIFRLASAGRPAPARERSRATNELARAVPQVTTVLVATWL
                     TNARGCVVYRGNAFPSNYLGNAFIADPSAHVIHRVVLREAGLDLAAARAPDEKSTEFI
                     LSSDPAFRPTQIINGPDGALYVADMRDGLDQGRIYRIVPVDFQR"
BASE COUNT          344 a          648 c          622 g          400 t
ORIGIN      
        1 gaagccgtag ttgtacatct tgtgctccca atgcatgagg accggcaccc ggtaatcggc
       61 tgccacttga tagagcttct gcatctccgg cgaatcgcac tcgacgccga acttcagctc
      121 ggcgatagcg accccgccga gctttaaata cttctcgatc tcctgcaccg cgtccggcag
      181 gtccggcacc tcgcacgcag cgaacaagta atgtttggga tgcgctttgg cgaagcggta
      241 gcagaactgg ttgccgaggc attgggcctg cagcccattg gagacgccgt tatgcgtcga
      301 agcggagttc gccgaccggc cggcgggcag cagaatcgtt ttcgtaacgc ccatagcgcg
      361 ttggtgagca aacagaacct catccgatcg accggaatag ccgacgtgct ggtggatgtc
      421 tatgatcggc tcgtagccca gcccatcctc cgtggtctta catcccggca tggccgccgc
      481 cagcgccagg agcgctgagc tggccaggaa ctctcggcgg gtgagcctcg gccgttgagg
      541 gttgagggtt gggggttggg ggtcaagcga ggtggaggag gtgttcatac cacttgcggg
      601 ctgccgttcg tcacgattca ttgtgggcta agtttgggga tgggaccaag ttagttcaag
      661 gcgttttccc tttcctgcct ggctgaattg gacgaagttt gacacatgag caatctgcgc
      721 aggaaaggtg gagaagggat gctgaccggc tctgccgtcg tggcggtcac ggcctgcttc
      781 ttcctttcgt gcaccggttg gtccgctgaa ccgaagccgg agtccaatgt tgtaccctac
      841 gtcgcgtcgg tcccagccac gaatgcgctg gccgccttcc gcgtcaggcc gggttttcgc
      901 ctggagctgg tcgcgcgcga gcccgcggtt atttcaccag tcgcgatggc gtttgacgag
      961 aacaaccgct tgttcgtcgt cgaaaggcgc gaggattcca gcgcgcccgg cacaaacgcc
     1021 cattccggcc gcatccattt gctggaagat accgaggggg acggcgagtt ccacctgagc
     1081 acggtttacg cggacaacct gccttgggcc tccgcggtgg cctgttgcag cggcggcgtc
     1141 tttgtcgcga cgagcccgga cattatcttc ttaaaggata ccaaaaccaa tggtatcgcg
     1201 gatgtgcgca aggccatctt taccagcttc accggcacga acctgctagg cgcacgggcg
     1261 ctgccgaaca atttcaattg gggcatggac aaccgcatcc acgccgcatc cgcagggata
     1321 gctgccttcg tgccggagtc aggcgcgcct ggcgcccccc tcgtttcctt gaccggcgcc
     1381 gatttctcct tcgatccgcg cgcactcgcg atgtgcgcgg aagccggccc cgccgaatcc
     1441 ggcctgagct ttgacgattg gggacgcaag ttcacctgcg atcccctgcg cccgttgcgc
     1501 tcaccgaggt atgagccgcg ttacctggcg cgcaacccct ttgtcccgcc accgccgcag
     1561 atgatcgagg tggccagccc cgccactccc atcttccgac ttgcctcagc gggacggccc
     1621 gcgcccgccc gtgagcgctc ccgggcgacg aacgagctgg cccgggccgt gccacaggtc
     1681 acaacggtgc tggtggcgac ctggctcacg aacgcgcgag gttgcgtggt ttatcgaggc
     1741 aacgccttcc cctcgaacta tctcggcaac gccttcattg ccgatccctc cgcccatgtc
     1801 attcaccgcg tcgtgctgcg cgaagccggg ctggacttgg ccgccgcccg ggcgccggac
     1861 gaaaagagca ccgagttcat tctgtcatcg gaccccgctt ttcgtcccac gcagattatc
     1921 aacggacccg atggcgcgct gtatgttgcg gacatgcggg acggcctcga ccagggccgc
     1981 atttaccgca tcgttcccgt ggacttccag cggc
//
LOCUS       DATCGS010000449         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_632060, whole genome shotgun sequence.
ACCESSION   DATCGS010000449 DATCGS010000000
VERSION     DATCGS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632060"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VIL39_09245"
     CDS             <1..785
                     /locus_tag="VIL39_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5481650.1"
                     /translation="ILQRQATVLPAIPQFYRSMVNAPMHTPLPLRVCISGAAPLPAQV
                     LQEFEAKFHVPLIEGYGLSEASPVVTKNPLDGTRKAGSIGLPIPNVEVSIQDDAGRHL
                     GTGESGEVCVRGGNVMLGYWKQPDETAKVMRDGWLLTGDIGYRDDQGYYFITDRKKDM
                     LLVNGINVYPREIEEILYQFPGVKEAAVVGKPNPRKGEQPIAFVAANDGVVLEEQALL
                     HFARHKLADYKVPRKVVFMPALPRNAAGKILKTTLRDLPAPS"
     gene            1612..1980
                     /locus_tag="VIL39_09250"
     CDS             1612..1980
                     /locus_tag="VIL39_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY5481651.1"
                     /translation="MIEREIQKLELENGRVPFDDLPPNMRLKNYREDLLERLKDADYA
                     AEYLAQVLAEKDRAAFLVALKDVVEATGGISAMAKRVGLKRPSLYKVFSKRGNPTLET
                     LQAILEALGLRVTVATAKTA"
     gene            2338..2511
                     /locus_tag="VIL39_09255"
     CDS             2338..2511
                     /locus_tag="VIL39_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481652.1"
                     /translation="MKPSPEPRETISRFEKLLAALVTNRVDFAVVGGLAVIFNGYPRL
                     TSDLLTYPNFSIA"
BASE COUNT          523 a          840 c          842 g          510 t
ORIGIN      
        1 agatcctcca gcgccaggcg acggtcctcc ccgccattcc ccagttttac cggagcatgg
       61 tcaacgcccc gatgcacact cccctgccct tgcgcgtctg catcagcggc gccgctccgt
      121 tgcctgccca ggtactgcaa gagttcgagg ccaagtttca cgtcccgctc atcgagggct
      181 atgggctgag cgaagccagc cccgtggtga ccaagaaccc gctcgacggc acgcgcaaag
      241 cgggctccat cggcctgccc atcccgaacg tggaagtcag catccaggac gatgccggac
      301 gccacctcgg gaccggcgaa agcggcgagg tatgcgtgcg cggcggcaac gtgatgctcg
      361 gctactggaa gcagccggac gaaaccgcca aagtcatgcg cgacggctgg ctcttgaccg
      421 gcgacatcgg ctaccgcgac gaccaaggct actacttcat caccgaccgc aagaaggaca
      481 tgctgctggt caacggcatc aacgtttatc cgcgtgagat tgaggaaatc ctctaccagt
      541 ttccgggcgt gaaagaagcg gcggtcgtcg gcaaacccaa tccgcgcaag ggcgagcagc
      601 cgattgcctt cgtcgcagcc aatgacggcg tcgtgctcga ggaacaggcg ctgctgcatt
      661 ttgcacggca caaactggcc gattacaagg tcccccgcaa agtggtgttc atgccggccc
      721 tgccccgcaa tgccgccggc aagatactga agaccaccct gcgcgacctg cccgcgccgt
      781 cctgatgacc gggagcggag gagcccgccg ctgccaacct gaggggaaag tcgtcctatg
      841 gatgcccggc gctggcgggg ccaggtcgcc caggctgtct ggacctcacc cgggaggcgt
      901 cgttagtgga ccttgccgcg gggttggcgg cgggggtcgt tggcgtttgg gcagccactg
      961 gcggtcgggc cctggcaggg cgcggtgccg atgaggagcc gccatccaaa gcgtccgaca
     1021 cgtccattgc ctcgttcact tcgcccaggg ggccagcccg gccgccattg gcggcgggcg
     1081 cagcttgggt cggtttggaa tcagcgctgc cctggatctt gctcagcaag gatggcacgt
     1141 aaaggtaacc tgccccaccc agcgcggcta acacgatggc atagattgca tatttcagca
     1201 gggggctctc acttttcggc gggcgcgccg cgagttgtct ggatggcatc ggtcctgggg
     1261 ccgtcgaacg cgcaacctgg gttaccccgg cagaaatctt ggggccctgg gacactggcg
     1321 gttgcgggac cagcgatcgg ggaacgggcg cagaggccgc cggaggcggc agagccccgg
     1381 gcacggtgag ggtcgtggcg caggccgggc attggatctg acgccctgac cactgttcgt
     1441 tgccggagat gtgctgcccg cattgggggc aggagaattt gatttcagcc ataagcgttt
     1501 tccaccacgc cgcaggcgga cacctggaga gctttcacga cgacagcgtg catcccccat
     1561 ttactgccgg gccggacgga ggtgtcaagg aagtacgcgg cccataaaac agtgatcgag
     1621 cgcgaaatcc agaagctgga actggagaat ggccgagtcc cgtttgatga cttgccacca
     1681 aacatgcgtc ttaagaatta cagagaagat ttgctggaac ggctcaagga tgcggactat
     1741 gccgccgagt atctggccca ggtcctggcg gaaaaggaca gagcggcctt cctggtagcg
     1801 ctgaaagatg tggtggaagc caccggcggc ataagcgcga tggccaagcg cgtgggactc
     1861 aaacgcccga gcttgtataa ggtcttttcc aagcgaggca accccacctt ggaaacgctc
     1921 caagccatcc tggaagccct gggcctgcgc gttaccgtcg ccactgccaa aaccgcgtaa
     1981 gcaacatcct gccgtcacta accggttgcg gccatgcggc acttcgggcc aagctttcca
     2041 acgggcaggc cctcggttga atgcagggca gcgcgtcccg cctgtccccg agtttctatt
     2101 gtcatttccg cttcgcgtct tcccgtcttc gctgttaact ccctttccac ttatgatcgc
     2161 accggccttg aatcgccagt tgacctccag ccgccggaac gactaagttt gccttaatga
     2221 aaaccggcgt tgaaccaatg cagcagccga ttcgcaagat ggtggggcgt ccgcctacgc
     2281 cgtctcgccg tctcgctcat ctggaagacc tggccgcttt tctcaaaaag cattcgcatg
     2341 aagccttcgc ccgagccgag ggagactatt tcccgcttcg aaaagctgtt ggccgccctc
     2401 gtcactaacc gcgtggattt cgcggtggtg ggtggtttgg ccgtcatctt caatggctat
     2461 ccgcggctga cctcagacct cctgacatac cccaactttt ccattgcgta ggctgcatgg
     2521 cttggtctcg ctccgagaat ggagtttctg gcaacgaatt cctcaggcca gtggattttc
     2581 aaacacgatc cgtaattggg tgcctgggaa aagagggttc agttggttga gactctcaca
     2641 aagtttggcg acggctcggg tgcggttggg ggaggtttgc ggagccaact tcacccaaat
     2701 ctgacccgtt tgtag
//
LOCUS       DATCGS010000450         3136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_632065, whole genome shotgun sequence.
ACCESSION   DATCGS010000450 DATCGS010000000
VERSION     DATCGS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632065"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1607)
                     /locus_tag="VIL39_09260"
     CDS             complement(207..1607)
                     /locus_tag="VIL39_09260"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5481653.1"
                     /translation="MDNDNDSTDLPGQPAAGGPPTTKPSRLTMIMEIFGVSRSITIAA
                     ILFAILVLAGAVFLFVQSAPPGTITITSGPENSIFNANALRYASILARYGVTLKVLTS
                     EGSLENLNRLCDPSFRVDIGFVQGGMTNAAADKLVSLGSISHQPLLVFYRGAPVELLS
                     GLAGKRIAVGPEGSGTRSLALSLLEINGIKPGGTNTILDWEPKQASKALINGTVDAVF
                     LMGEDASLAVIIELMRAPDVHLLNFRQATAYTRRFSYLGVIEFPQGVLDLGHNIPPQD
                     LYLVGPTVELIAREDLHPALSDLLLGAAREVHGNANLLQRKGEFPAPLEHDIPISADA
                     ARFYKSGKGFFYRYLPFWLASLTSRIVVVFVPTIVVLIPVLRSIPSCYRWRIRSRIYR
                     WYRALLSLERELLGEADPGKREQLLRRLDEIEKAVNQMKVPAFFADQFYSLRGHIGFV
                     RLMVGDTRQRPKVEKS"
     gene            complement(1608..2573)
                     /locus_tag="VIL39_09265"
     CDS             complement(1608..2573)
                     /locus_tag="VIL39_09265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5481654.1"
                     /translation="MNRRQFLEAMAVGLVAAPALGSLPTGKLPTRTLGRTGIKVPILG
                     FGSGSRFLMYKDEDKAIEALNRAIDLGITYIDTASSYGDGQSEERIGRVMATRRKEVT
                     LSTKIFARTADEGRRQIELSLKRLRTDHLDVLHIHQLKGLEDLAAIEARGGVLEALYE
                     AREHKTARAVGITCHAEPAALRIALERHDFDCTQMALNAGLARMADAPGGMKATLMGS
                     SGFEGLALPVATRKHMGVIAMKVFGQEQLIGAASAEELLTYALSLPVSLASVGMPRIE
                     HIERNAVLARAFTPMSTRERRRLQQSIESGRKLAMRQFFSDHEDV"
     gene            2739..3002
                     /locus_tag="VIL39_09270"
     CDS             2739..3002
                     /locus_tag="VIL39_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY5481655.1"
                     /translation="MTAHIYGRGQMVIPARARKEAGFGRGDVVLVEPEGDGRILLTRL
                     ERSKPPGPSNARLVRRKGKHSVIVGGPKIANEQIRQILNDEFP"
     gene            2999..3133
                     /locus_tag="VIL39_09275"
     CDS             2999..3133
                     /locus_tag="VIL39_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481656.1"
                     /translation="MIYLLDVNVLLAAIIQNHPNHAVTDAWLRNKLPFTALKRRGREN
                     "
BASE COUNT          610 a          938 c          977 g          611 t
ORIGIN      
        1 atgtccgcgc tccttcggtt gcgtctttgc ttcctggcgt ctttgcgtta accgccgttt
       61 ttgggttcat attcatgtcc agttacaaac tgactttcct ttcattagac ggcaaaggat
      121 ggtttgtgct ccgggaaggg cgttggcaag cagtttcggc ggctggaata cggcgacacc
      181 aggcaagttc cgaaacctgt ctgccctcag gatttctcaa ctttaggccg ctgccgcgtg
      241 tcgcccacca tcagacgaac gaaaccgatg tggccgcgca ggctatagaa ttggtcggca
      301 aagaaggccg gcaccttcat ctggttgacg gctttctcga tctcatccaa ccgcctcagc
      361 agttgctcgc gtttgccggg gtccgcttct cccaagagct ccctttccaa ggacagcaag
      421 gcccgatacc accggtagat gcgcgaccga atccgccagc gatagcagga cgggatgctg
      481 cgcaagaccg gaatcagcac cacaatggtc ggcacaaaga ccaccacaat ccggctggtc
      541 agactggcca gccagaatgg cagatagcga tagaagaacc ctttgcccga tttgtagaag
      601 cgggcggcgt cggcactgat ggggatgtca tgttcgagcg gggcggggaa ttcccctttg
      661 cgctggagca gattggcatt gccgtgcact tcgcgcgccg ccccgagcag caagtcagac
      721 agcgccggat gcaagtcttc gcgcgcgatc aactccaccg tcggaccgac cagatagaga
      781 tcctggggcg gaatgttgtg gccgagatca agcaccccct gcgggaattc gatcacgccc
      841 aggtagctga accgccgggt gtaagcggtc gcctgccgga agttcaacag gtgaacgtcc
      901 ggcgcccgca tcagctctat tatgaccgcc agcgaggcgt cctcccccat caggaacacg
      961 gcatcgacgg tcccgttgat cagggccttg gacgcctgtt tcggctccca atccagtatc
     1021 gtgttcgtac cgccgggttt aatgccattg atttcgagca aactcagcgc cagcgagcgg
     1081 gtgccgctac cctctgggcc gacggcgatg cgcttcccgg ccaggccgga cagcaactcc
     1141 accggcgcac cgcggtagaa gaccagcagc ggttgatgag aaatgctccc aagcgaaacc
     1201 agcttgtcgg ccgccgcatt ggtcatgccg ccctggacga agccgatgtc cactcgaaac
     1261 gaaggatcac atagccgatt gaggttctcc agcgagccct cggaggtgag caccttcaac
     1321 gtcacgccgt agcgcgccag aatgctggca tatcggaggg cgttcgcgtt gaagatgctg
     1381 ttttcaggac cgctcgtgat agtgatcgtg cccggcggag ctgactgtac aaagaggaac
     1441 accgcgccgg ccagcacgag aatggcaaag aggatcgctg caatggtaat agagcggcta
     1501 acgccgaata tctccatgat catcgtcagc cggctcggct tggtggtagg aggccctccg
     1561 gcagccggtt gaccgggcaa atcagttgaa tcgttatcgt tgtccaatca gacgtcctcg
     1621 tggtcgctga agaactggcg catcgccagc ttgcggccgg attcgatgga ctgctgcagc
     1681 cgtcgtcgtt cgcgggtgga catgggtgtg aaagcgcgcg ccagcacagc gttgcgctcg
     1741 atgtgctcga tccggggcat gccgacgctc gccaggctga caggcaggga tagggcgtag
     1801 gttagcagct cctccgccga ggctgccccg atcagttgtt cctggccgaa caccttcatg
     1861 gcgattacgc ccatgtgttt gcgcgtggcc accggcaggg caagcccctc gaagccgctg
     1921 ctccccatta gcgtggcctt catcccgccg ggcgcgtcgg ccatgcgggc caaaccggcg
     1981 ttgagcgcca tctgcgtgca atcaaaatca tgccgctcca gggcgatgcg cagggccgcc
     2041 ggctcagcat ggcaggtgat gccgacagcg cgagccgtct tgtgctcgcg ggcctcgtac
     2101 aaagcctcca gcaccccgcc gcgcgcctcg atggcggcga ggtcctcgag gcccttgagc
     2161 tggtggatgt gcaggacatc gaggtgatcc gtgcggagac gtttcaggct gagctcgatt
     2221 tgacgccggc cctcgtcggc ggtgcgcgcg aagatcttgg tggacagggt cacttccttg
     2281 cgccgggttg ccatgacccg gccgatgcgt tcctcgcttt ggccgtcgcc gtacgagctg
     2341 gcggtgtcaa tgtaggtgat gccgagatca atcgcgcggt tgagcgcttc gatggccttg
     2401 tcctcgtcct tgtacatgag gaaccggctg ccggagccaa agcccaggat cggcactttg
     2461 atgccggtgc ggcccagtgt gcgggtgggc agtttgccgg tcggaagcga gcccagcgcc
     2521 ggggcggcga ccagccccac agccatggct tcaaggaatt gccgacggtt catgcgtggc
     2581 ggccagcatg ttgcatccac cctcgcggac acaaggagaa agaccggttc attgtaatgg
     2641 gtgacccatt actcgcgcgc gtatcggtgg ctgccacgca ctgggtgaca gattcctgag
     2701 agcttgctcg taagcgtcgc ttactatatc ctcttcgcat gacagcgcac atctatggca
     2761 gaggacagat ggtcattccg gcccgggcga ggaaggaagc tggcttcggt cgcggcgacg
     2821 tggttttggt tgagccagag ggggacggcc ggattctatt gacccggttg gaacggtcca
     2881 agcccccagg accgtcgaat gcgcgattag tgcgtcgtaa gggcaagcat tcggtcatcg
     2941 tcggcggtcc gaagatcgcc aacgagcaga ttcggcaaat cctgaacgac gagttcccat
     3001 gatttacctg cttgatgtaa atgtgctgct ggcggccatc atccagaacc atcccaacca
     3061 cgcggttacc gatgcgtggc tcagaaacaa gttaccgttc acagccttga agcgcagagg
     3121 ccgggaaaat taatta
//
LOCUS       DATCGS010000451         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_632066, whole genome shotgun sequence.
ACCESSION   DATCGS010000451 DATCGS010000000
VERSION     DATCGS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632066"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VIL39_09280"
     CDS             complement(<1..1189)
                     /locus_tag="VIL39_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5481657.1"
                     /translation="MSHAEFVHLHLHTEYSLLDGACRLDRLMDKAHELKFPALAITDH
                     GVLYGAIDFYEAARERGITPIIGCEVYVAPGSRLEKKSSTGGRDVYSHLVLLAKDEAG
                     YKNLIKLSTAAHLEGYYYKPRIDKELLAAHKDGLIALSGCLASEIPEWITKDQLPKAR
                     ETIDWFKQTLGAENFYLELQNHGLPEQAKVNRHLIAWAKEFGLKLVATNDVHYVEKSH
                     SHAHDCLICIGTQTQLTDSKRMKYTEQQFYLRSAEEMKARFAEVPEAVQNTLEVAEKC
                     NLEIEFGKLHYPVFHPPEHFTREGYLRHLLADGLLKRYGIQARAEEHGFTVERVEDPL
                     RLPTYQAVQSPETQSPAADHPVTDHGSRITLHASCDLSDPAVAAAVKAIINRLQLELK
                     VIEK"
     gene            complement(1379..2488)
                     /locus_tag="VIL39_09285"
     CDS             complement(1379..2488)
                     /locus_tag="VIL39_09285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5481658.1"
                     /translation="MKKITPSLMLLLLAAPLPMWGAAPDRPAATNAAAAPKPAAKTAN
                     LFSDKLVAKGKGLEINRSQLDDEIIRFKAQVAGRNQAVPPEQMALMERQLLDQLIQVQ
                     LLRAKATDADKAAGKALADKRFEEAKTKLGSEDALNRQLKLMGVTREEVQTKWTESAT
                     AEAVLKRELNVSVTDADTKKFYDENPARFEEPEKARASHILLTTTDPKTNTELTEDQK
                     AAKRKEMEGLLKRARAGEDFAKLAKEYSEDPGSKDKGGEYEFPRGQMVPEFEAAAFSL
                     GTNQVSDIITTRFGYHIIKLSEKIPARKVEYAKAAADIKEGLTQQALQKQFPDYIAKL
                     KKEAGVEILDEKLKAKEPTDSPTPASQAPGKPEAK"
     gene            complement(2513..>3219)
                     /locus_tag="VIL39_09290"
     CDS             complement(2513..>3219)
                     /locus_tag="VIL39_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5481659.1"
                     /translation="EVISGYLVTGEVRNAVNLPYLDAKTYEQIKPYLVLGEKLGHLLA
                     QLAPPQVDRLRITFGGKAQELPNIDPVTRAVLLGFLSRVSVKDLNNVNVRSIASTLGL
                     TVEEKRSNEPVTFNEWLHVQAFRNGEKVISAGGTFFGSPDNPRIVRVFSQPTEIVPFG
                     VVLLLSNKDRPGMVGYLGTLLSRHKVNIASMSLTRDIEGGQALTVLNLDSVPPQEVLD
                     EIQRDPDISNVKVVTL"
BASE COUNT          561 a          980 c          954 g          724 t
ORIGIN      
        1 tcttctcaat gactttgagc tcaagctgga ggcggttgat gatggccttg acggcggccg
       61 cgacggcggg atcggagagg tcgcatgaag cgtgaagcgt gatccgtgat ccgtgatccg
      121 tgaccgggtg atcggcggct ggtgactgag tctcgggact ttggactgcc tgatacgtcg
      181 gcagccgcag cggatcttcg acgcgctcga cagtgaatcc atgctcttcg gctcgcgcct
      241 ggatgccgta gcgtttgagc aatccatcgg ccagcaaatg ccgcaaatag ccttcgcgcg
      301 tgaagtgctc gggtggatgg aataccgggt aatgcagctt cccaaactcg atttccaggt
      361 tgcacttctc ggctacctcc agcgtgttct gcaccgcctc cgggacctcg gcaaagcgtg
      421 ccttcatctc ctccgccgag cgcaagtaga actgctgctc ggtatatttc atccgcttgg
      481 agtcggtcaa ttgcgtctgc gtgccgatgc agatgaggca gtcgtgggcg tgtgagtggc
      541 tcttctcgac gtaatgcacg tcgttcgtcg cgacgagctt cagcccgaac tccttcgccc
      601 acgcaatgag gtggcgattg accttggcct gttccggcag gccgtggttt tgaagctcca
      661 ggtagaaatt ctccgccccg agcgtttgtt tgaaccagtc aatcgtctcg cgcgccttcg
      721 gcaactggtc cttagtaatc cactccggga tctcgctcgc caggcagccg gagagcgcga
      781 tcagcccgtc cttgtgcgcc gccagcagtt ccttgtcgat gcgcggcttg tagtaatagc
      841 cctccagatg ggccgccgtg gagagcttga tgaggttttt gtagccggct tcatccttgg
      901 ccagcagcac gagatggctg taaacgtctc ggccgcccgt gctggacttc ttctccaacc
      961 ggctcccggg cgcgacataa acctcgcagc cgatgatcgg cgtgatgccg cgctcgcgcg
     1021 ccgcctcgta aaaatcaatc gccccgtaaa gcacgccgtg gtcggtgatc gccagcgccg
     1081 gaaacttcag ctcatgcgct ttgtccatca accggtccag ccggcaagcc ccatcgagca
     1141 atgagtactc ggtgtgcaaa tgaagatgaa caaattccgc atgcgacatg gaagcaatct
     1201 acggcgggtc aaccgtgccg ggcaagaagc gactggcgag cgcaagcctg agaatccaag
     1261 tcaccgcgtc gatggttcag cggcagcggc aatggcaatc tgaagccgat gcgtccggcg
     1321 gcaggccaat aaaaaaaggc ccgactcgcg tcgggccctg tctcacgaat taaagaggtt
     1381 acttggcttc cggcttccca ggtgcctgac tagccggcgt cggtgagtcg gtcggctctt
     1441 tcgccttcag tttctcatcc aggatttcca ctccggcctc cttcttaagc ttagcgatat
     1501 agtcggggaa ttgcttctgc agcgcctgtt gcgtcagccc ctccttgatg tcggcagccg
     1561 ccttggcata ctcgaccttc cgagccggga tcttctcgct cagtttgatg atgtgatagc
     1621 cgaaccgcgt ggtaatgatg tcgctgacct ggttggtgcc cagcgagaac gccgccgcct
     1681 cgaattccgg caccatctgg ccgcgcggga actcgtattc gccgcctttg tccttcgagc
     1741 ccgggtcttc ggaatactcc ttggccagct tggcgaaatc ttccccggca cgggcgcgct
     1801 tcagcaggcc ctccatttcc ttgcgcttgg ccgctttctg gtcctccgtc aattcagtgt
     1861 tggtcttggg gtcggtcgtc gtcaggagga tatggctggc gcgggccttt tccggctcct
     1921 cgaatcgcgc cgggttctca tcataaaact tcttggtatc ggcgtcggtg acactaacat
     1981 tcagttcgcg cttgagcacg gcctctgcgg tcgcgctctc ggtccacttt gtctggacct
     2041 cttcgcgcgt cacccccatg agcttcaact gccgattgag ggcgtcctcc gagccgagct
     2101 tggtcttggc ttcctcgaat cgcttatcgg ccagcgcctt gcccgccgct ttgtcggcat
     2161 ccgtggcttt ggcccgcaga agctggacct ggataagctg atcgagtagc tgccgttcca
     2221 tcaacgccat ctgctccggt gggacggctt ggttgcggcc cgccacctgc gccttgaacc
     2281 ggatgatctc gtcatccagt tggctgcggt tgatttctag ccccttgcct ttggccacaa
     2341 gcttatcaga gaagaggttg gcggttttgg ccgccggctt tggtgccgcg gcggcgttgg
     2401 ttgcggccgg ccggtcaggc gcggcgcccc acattgggag cggcgcagcc aggagaagca
     2461 gcatgagaga cggtgttatt ttcttcataa atatattctt tagttcgcta aattataatg
     2521 tgaccacctt gacgttactg atgtccgggt ctctttgaat ctcgtccaac acttcttgcg
     2581 gcggcacgct atccaaattc aaaaccgtta aggcctggcc tccctcaata tcgcgggtga
     2641 ggctcatgct ggcaatatta accttgtggc ggctcaacag cgtgccgagg tagcccacca
     2701 tgcccggccg gtctttgttg ctgagcagga gcaccacgcc gaagggcaca atctccgtcg
     2761 gctggctgaa cacgcgcacg atgcgcggat tgtccggcga cccgaagaag gtgccgccgg
     2821 cggaaatgac cttctcacca ttgcggaagg cctgcacgtg cagccactcg ttgaacgtca
     2881 ccggttcatt cgagcgcttc tcctccaccg tcagcccgag ggtcgaggcg atgctccgca
     2941 cgttaacgtt gttcaggtct ttcaccgaga cgcgcgaaag gaaccccagc aggacggcgc
     3001 gcgtcaccgg gtcaatgttg ggcaattcct gcgccttgcc gccaaaggtg atgcgcaggc
     3061 ggtccacctg cggcggggca agctgggcca gcaaatgacc gagcttctcg ccgaggacaa
     3121 ggtagggctt gatctgctcg taggtcttcg cgtccagata cggcaggttg acggcattgc
     3181 gcacttcgcc ggtaaccagg tagcccgaga tgacctcgg
//
LOCUS       DATCGS010000452         3682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_335591, whole genome shotgun sequence.
ACCESSION   DATCGS010000452 DATCGS010000000
VERSION     DATCGS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335591"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1480)
                     /gene="thrC"
                     /locus_tag="VIL39_09295"
     CDS             complement(134..1480)
                     /gene="thrC"
                     /locus_tag="VIL39_09295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5481660.1"
                     /translation="MSYRAWFQCINPQCGATYPLNSIVYQCQQCQSLLEVQHDTAALA
                     ARGAAAWMKLFEDRYKSNEWPYGSGVWGKKEWILPQISDHNVVSLYEGGTNLFWAERF
                     GKVIGVQDLWIKLCGNTHSGSFKDLGMTVLVSQVKQMISEGAPIKAVVCASTGDTSAA
                     LATYCAAAGIQSVVLLPRGKISIAQLVQPISNDALVLYLDTDFDGCMRLVQEITKDPT
                     LYLANSMNSLRVEGQKTVGIEIVQQFDWEVPDVIIIPGGNLGNVSALGKGLLMMKDLG
                     LIAKLPRIVVAQAERANPLYRSYLRNFESFEPMQAQKTLASAIQIGNPVSFEKAVRTL
                     KQFNGIVEQATEEELADAAALGDRTGMFNCPHTGVALAVLIKLLKAGKISKSERVVVI
                     STAHGLKFVEMKVKYHQRELEFPSRHANQPIELPARLDAVKEALHQALETKSQLRF"
     gene            complement(1786..3312)
                     /locus_tag="VIL39_09300"
     CDS             complement(1786..3312)
                     /locus_tag="VIL39_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481661.1"
                     /translation="MGCPVLRGQTIIQSFPGLSLHDADALGTGSTPPDTMGAAGTNQF
                     VEFINGAFAIYSKMGVRQTLISDNAFWENAGISASTISAGLTDPRVLYDVGSGRWFAS
                     EMTLDTIGAQLLLVRSATSDPGGAWQAVNFTSNIGTDSPDFDTLGVDSTGVYIGINNF
                     DALLNPAGVSFFSIPKADLVASTPSLARMTRFDNLADTVYGFTPQGVSNPDPGPGHGV
                     LLAIDNVGYKFFDRTTISGSGAAGATLGTTVRISNTYDAGPNAAAQPSGQKVDAGDDR
                     FSGSIRQVGSNIFMANCILQGGRDAVHWMVLKEPSNTLLGEGIISDASHDFFYPSIAA
                     NHSGRILLVFNRSGSSSPDGDIGIYAAAGSFTASSVTMGSPFLVRQGTVSDYHLSFDS
                     APYRWGDYSATMVDLTDENLFWTIQEIPAASTAWGTQITLISLATNRPSLTITRSGAN
                     LNLSWPASADPAYVLQAAPQLALSTTWTNVPNPVNIVINQNVVTVANTAAPRYYRLKK
                     "
     gene            complement(3403..>3682)
                     /locus_tag="VIL39_09305"
     CDS             complement(3403..>3682)
                     /locus_tag="VIL39_09305"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY5481662.1"
                     /translation="SCIVSIPNFGYHQLRRMLAETGRAPKSAGVLHYEWYNTPNIRFF
                     TIADFEDFCREKNIQVHRRIAIDTEAQAEVFDHPNLNADLAIFVISRS"
BASE COUNT          728 a         1082 c         1129 g          743 t
ORIGIN      
        1 atgaaagcgg cagaggacag ccgcactcca aaaccttgcg gaagaagcaa cgtgccgaaa
       61 aacgcgctag cgtcttggag tgcggctgtc ccctgccgct tttcggcgta gcgcgaattt
      121 gtgcaattag gcactagaaa cggagctgac tcttggtctc aagcgcctgg tgcagcgctt
      181 ccttcaccgc gtccagccgc gccggcagct cgatcggctg gttcgcgtgc cggctcggga
      241 actccagttc gcgctggtga tacttcacct tcatttccac gaacttcagc ccatgcgcgg
      301 tcgagatcac cacgacgcgc tcggatttgc tgatcttgcc ggccttgagc aatttgatga
      361 gcaccgccag cgccaccccc gtatgcggac aattgaacat cccggtgcgg tcgcccagcg
      421 ccgccgcatc ggcgagttcc tcctcagtgg cctgctcgac gatgccgttg aactgcttga
      481 gcgtgcgaac ggccttctca aagctgacgg ggttgccgat ctggatcgcg ctggccagtg
      541 tcttctgcgc ctgcataggc tcgaagctct cgaagttgcg caggtaggag cggtaaagcg
      601 ggttggcccg ttccgcctgc gccacgacaa tgcgcggcag cttggcgatc agtcccaggt
      661 ccttcatcat cagcagccct ttgccgagcg cggagacgtt ccccaggttg ccgccgggga
      721 tgatgatgac gtcgggcact tcccagtcga attgctggac gatctcgatg ccgacggtct
      781 tctggccctc cacacggagg gagttcatgg agttagccag gtagagggtc gggtctttgg
      841 taatctcctg gaccagccgc atgcagccgt cgaagtccgt gtccaggtac agcaccaggg
      901 cgtcattgga aatcggctga accaactgcg cgatcgaaat cttgcctcgc ggcaagagca
      961 ctaccgactg gatgcccgcc gccgcgcaat acgtcgccag cgccgccgag gtgtcgcccg
     1021 tcgaggcgca caccaccgcc ttgatcggcg cgccctcgga gatcatttgt ttgacctgcg
     1081 agaccagcac cgtcatgccc aggtctttga acgacccgct gtgggtattg ccgcagagct
     1141 tgatccaaag gtcctgcacc ccgatcacct tcccgaaacg ctcggcccag aagaggtttg
     1201 tccccccttc atacagcgac acgacgttgt ggtcgctgat ctgcggcagg atccattcct
     1261 tcttgcccca cacgccggag ccgtaaggcc actcgttgct cttgtagcgg tcctcgaaga
     1321 gcttcatcca cgccgccgct ccgcgggcgg ccagcgccgc cgtgtcatgc tgcacctcca
     1381 gcagtgactg gcactgctgg cattggtaca cgatggagtt cagcggataa gtggctccgc
     1441 attgcgggtt gatgcactga aaccaagctc gatacgacat ttcttaattc tcctgcgctc
     1501 caaggctgcg gccggcgaag gcccgcgccc agagcggcac aactcgaatg gagaccagca
     1561 tgaccaactg gccgaagcat ttcaaatccg aaatccgaag ccctaaactg gtgggcttgt
     1621 cttgacacca ccaccaagcc agcaggtccg cagcaggcga ttccggttag attaagggct
     1681 tcggggttga actgctcgtt ctgcttggcg taatgcttgt ctgaccatac cattctgcta
     1741 tggcgtatct gaaagccgag gaagagcagg aagcagctta gccgttcact tcttcaaccg
     1801 gtaatatctc ggggcggctg tgttcgcgac ggtgaccacg ttctggttga taacgatgtt
     1861 caccgggttg ggcacatttg tccaggtggt ggatagggcg agttgcggtg cagcttgcag
     1921 cacgtaggcc ggatccgcgc tcgcaggcca gcttagattc agattcgcgc ccgagcgcgt
     1981 aattgtcagg ctcggacggt tggtggccag cgaaataagg gtgatttgcg tgccccaagc
     2041 agtggacgcc gccggaatct cctggatggt ccaaaacaga ttctcatccg tgaggtccac
     2101 catcgtcgcg ctataatcgc cccaccggta aggggccgaa tcaaagctga gatggtaatc
     2161 actgacggtg ccttggcgca ccaaaaacgg tgatcccata gtgacgctgg aggcggtgaa
     2221 gctgccggcg gcggcataaa tcccgatgtc gccatcgggg ctgctcgatc cggaacggtt
     2281 aaagacgagg aggattctgc cgctatgatt ggcggcgatt gagggataga agaaatcgtg
     2341 gctcgcgtcg ctgatgattc cctctcccaa caaggtgttg ctgggttcct tgagcaccat
     2401 ccagtgcacc gcgtctctgc cgccttgcag gatgcagttg gccataaaga tgttactgcc
     2461 gacctggcgg atcgaccctg agaaccgatc atcaccggcg tccaccttct ggccactggg
     2521 ctgcgcggcg gcgtttgggc cggcatcgta ggtgttgctg atccgcactg tggtgccgag
     2581 cgtggcaccc gctgcaccgg agccggaaat ggtcgtgcgg tcaaagaact tgtatcccac
     2641 gttgtcaatc gcgagcagga ctccgtgccc ggggcctgga tcggggttgc taactccctg
     2701 gggggtaaac ccgtaaaccg tgtcggcgag attgtcgaac cgggtcatgc gagccaggga
     2761 gggtgtgctg gccacgaggt ccgccttggg gatggagaag aaggaaacgc cggcggggtt
     2821 aaggagggca tcgaagttat tgatgccgat gtaaaccccc gtggaatcca cgccgagcgt
     2881 gtcgaagtcc ggagaatcgg tgccgatatt actcgtaaag ttcactgctt gccaggctcc
     2941 gccggggtcg gaagtggctg agcgaacgag gagcaactgg gcgccaatgg tgtccaaggt
     3001 catctccgag gcaaaccagc ggccgctgcc aacatcatag aggactcgcg gatccgtcag
     3061 cccggcggaa atggttgagg cgctgattcc ggcattctcc caaaacgcgt tgtcgctgat
     3121 caatgtctgc cgaaccccca tcttgctgta gatggcaaat gccccgttga taaactccac
     3181 aaattggttg gtccccgccg cgcccatcgt gtccggcggc gtggagccgg tacccagcgc
     3241 atcggcatca tgcagggaga gcccgggaaa actttggata atcgtctgcc cgcgcaaaac
     3301 cggacagccc aatgcgatga ttgcagacgc caagcaccac ctggctgtcg caggaacaag
     3361 gttcatcata atatggggta ggcaattatc gcgtggacga ggctagctgc ggctgattac
     3421 gaagatcgcc aggtcggcgt tcaggttcgg atgatcgaag acttctgcct gcgcttcggt
     3481 gtcaatggca attcgccgat ggacctggat gttcttctcg cggcagaagt cctcgaagtc
     3541 ggcaatggtg aagaagcgga tgttgggcgt gttgtaccat tcgtagtgca agacacccgc
     3601 ggacttcggc gcccgcccgg tctcggccag catgcgccgg agctggtgat agccaaagtt
     3661 cgggatgctg acaatgcagc tt
//
LOCUS       DATCGS010000453         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_682398, whole genome shotgun sequence.
ACCESSION   DATCGS010000453 DATCGS010000000
VERSION     DATCGS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682398"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VIL39_09310"
     CDS             <1..984
                     /locus_tag="VIL39_09310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5481663.1"
                     /translation="VAANGLMFFGMSYLFGLTGTTSLTDILPRLQPVIHSPMAITGLA
                     LTFCGIYYKLAIFPFHFWTPDVYQGASNETAGLVASLPKVGAVAVLVRFVSLASPDNH
                     TVAIMLAILATASMFYGNLIALVQKDFKRLLGFSGIAHAGYALIGFVALDQFGYTAAL
                     YYIIGYVLMVLACFVVICKVSRDGTNVAIEDLAGLHKRSPLLAVTLIVGVFALAGIPP
                     FVGFMGKLSLLTAALSKGYIFLVIIAVINAAIAVYYYLGVVREAVFRDPGDRPPIHLD
                     WPTRVLCILLIGGILALGVAPARVLNALSTSVAAVTSATPPATSATANVTR"
     gene            complement(1098..1973)
                     /locus_tag="VIL39_09315"
     CDS             complement(1098..1973)
                     /locus_tag="VIL39_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481664.1"
                     /translation="MKILDTPRSGKCGQTVAFQSRFGLCLRQYVPQKAALTLARERVC
                     AVFGGNSRKWSVRLGEEQRDRWALAGAQVMSHPRLAQKGPLTGQQLWQAISTVRAIVG
                     LPETLEVPARPVFSHSNVGRLVIENGEDGARLYLTVSGELNEDIMVFGQEPCSRGRYK
                     RRNVSYLGLLPPPIGGLSEITRLYKAKFGEPRPGQKVFIVTCQEKDGWKGLDLETSAT
                     VPERPKDLQATAETANGQILYMHTGCTPDAKGIAVPVVGSSQASAEPADGGGDAAGAP
                     LEGKKVPGEAGDAPV"
     gene            2336..2551
                     /locus_tag="VIL39_09320"
     CDS             2336..2551
                     /locus_tag="VIL39_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481665.1"
                     /translation="MSTIAEIEAAVKALPHAQQEELFAFLAERIGSSSNGQAGAEDAF
                     APFIGAHAGPHEATGRKAEEILYGSAA"
     gene            2548..2949
                     /locus_tag="VIL39_09325"
     CDS             2548..2949
                     /locus_tag="VIL39_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5481666.1"
                     /translation="MSAPAFLDTGAIYALADRNDADHSAVRAIYLEANRRLVTHDLVM
                     VEAFSLLTKRLHKTAALQTIGALRRSSKVEIVPLTGDLLNAAWTRCERFADKEWDWID
                     CVSFELMERRGVREALSLDHHFAQAGFILLV"
     gene            3032..>3220
                     /locus_tag="VIL39_09330"
     CDS             3032..>3220
                     /locus_tag="VIL39_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEY5481667.1"
                     /translation="MIKELDCVALAADLPDHGLAVGDVGAVVHVYEGGHSYQIEFTTY
                     DGRTVAVAKVSADQIRSLG"
BASE COUNT          551 a         1068 c          925 g          676 t
ORIGIN      
        1 gtcgccgcca acggcctgat gttcttcggc atgagctacc tcttcggcct gaccggcacg
       61 acctcgctga ccgatatcct cccccgcctc cagccggtga tccattcccc catggccatt
      121 accggccttg ccctgacctt ctgtgggatt tactacaagc tggcgatctt tcccttccac
      181 ttctggaccc ccgacgtcta tcaaggcgcg tccaacgaaa ccgcgggtct ggtcgcctcg
      241 ttaccgaagg tcggcgcggt ggcggtccta gtccgcttcg tttctctggc gtcgccggac
      301 aatcacaccg tcgccatcat gctggccatt ctcgcgaccg cctccatgtt ctacggcaac
      361 ctcatcgccc tggtgcagaa ggacttcaag cgattgctgg gattctccgg catcgcccac
      421 gccggctacg ccctgatcgg gttcgtcgcc ctcgaccagt ttggctatac cgctgctctg
      481 tactacatca tcggctacgt cctcatggtg ctggcctgtt ttgtggtgat ctgcaaagtc
      541 tcgcgcgacg gcaccaacgt cgccattgaa gacctcgccg ggctccacaa gcgctcgccc
      601 ttgctcgccg tgaccctgat cgtgggtgtg ttcgcgctgg ccggcatccc tccgttcgtc
      661 ggtttcatgg gcaagctcag cctgctcacc gcagccctga gcaaaggcta catcttcctc
      721 gtcattatcg ccgtcatcaa tgccgccatc gccgtctatt actacctcgg cgtcgtccgc
      781 gaagccgtct tccgcgatcc cggtgaccgc cctccgatcc acctcgactg gccaacccgc
      841 gtgctgtgca tcctgctgat cggcggcatc ctcgccctcg gtgtcgcccc cgcccgcgtc
      901 ctcaacgccc tctccacctc ggtagctgcc gtcactagcg ccacgccgcc agcaacctcc
      961 gccaccgcca acgtcactcg ctgacgccag cgcctttcgc ggttgctggc tattggccat
     1021 tggctattgg ctgtctctct cggcgcctcc gacccatgcc agagttatct cggcggtttt
     1081 ggcgttccca caccgcctca aacgggagcg tcgcccgcct ccccaggcac tttcttcccc
     1141 tccaaaggcg ctccggcggc gtctccaccc ccatctgctg gctccgcact ggcctgggac
     1201 gagccaacca ctggcactgc aatcccctta gcatccggtg tgcatccggt gtgcatatag
     1261 agaatctggc catttgctgt ctcggcggta gcttgaaggt cctttggccg ttccgggacg
     1321 gttgcgctgg tctccagatc cagtcccttc cagccatctt tttcctggca ggtgacgatg
     1381 aagaccttct ggccggggcg cggttcgcca aacttggcct tgtagaggcg ggtaatctcg
     1441 ctcaagccgc caatcggagg cgggagcagg ccgaggtagg aaacgttccg gcgcttgtag
     1501 cggccgcggc tgcacggctc ctggccgaat accataatgt cctcgttcag ctcccctgaa
     1561 accgtcagat aaagccgcgc cccatcctcg ccgttctcga tcaccaaccg ccccacatta
     1621 ctgtgagaaa acaccgggcg agcggggacc tccagggttt ccgggaggcc cacgatggcc
     1681 cgcacggtac tgatggcctg ccagagctgc tggcccgtca gcggcccttt ttgggcgagc
     1741 ctggggtggc tcatcacctg agccccggcc agagcccagc ggtcccgctg ttcctcgcca
     1801 agcctgacgc tccacttccg agagttgccg ccgaagaccg cgcacacgcg ctcacgcgcc
     1861 agcgtgaggg cggccttttg tgggacgtat tgcctcaggc acaaaccaaa gcgtgattga
     1921 aaggccacgg tctggccgca tttcccggac cggggggtat ccagtatctt cataaggtag
     1981 tctccaaatt acatgcactg tgttgccggc tgggcaagtt gacggagttt cgtccctggc
     2041 cggtttcctc ggcccgggcc cgtcactgcc tcaacctaat gcccaagtat agtcgaaaac
     2101 gcagaggaat caacggcaag ccgccaaggt gtggagacgc gggtaggccg ttttagccac
     2161 ggatggcacg gatttcacgg attaaggaca gggaatatga cccgcgaggg ccgccaggaa
     2221 atcctccgtg ccgcctttaa tgaacgggac tgccgcagcg cccaggggca ggtcaccgaa
     2281 tgtgctgtct gaccttcggc ttgcgtaacg agcggtcggc ggttagagtt tcgccatgag
     2341 tacgattgcc gaaatcgaag ctgctgtgaa ggcgctgccg catgcgcagc aggaggaatt
     2401 gtttgctttc ctcgcggaaa ggatcgggag ttcttccaac ggccaggctg gtgcagagga
     2461 tgccttcgcc ccgttcatcg gcgcccatgc cgggccgcat gaagcgaccg gacggaaggc
     2521 cgaggaaatt ctttacggaa gcgcggcgtg agcgcgccag cttttctgga cacgggagcg
     2581 atctatgcgc tggcagatcg caacgacgcc gaccattcgg cagtgcgcgc gatttacctc
     2641 gaagccaacc ggcgcctggt aactcacgat ttggtgatgg tggaggcttt ctcgctgttg
     2701 acgaagcggc tgcacaaaac ggcggcgctt cagaccattg gagctttacg ccgttcctca
     2761 aaggttgaga ttgtgccgct gacgggcgat ctcctcaacg cagcctggac gcgatgcgaa
     2821 cgatttgccg acaaggagtg ggattggatc gattgtgtca gcttcgaact catggagcgc
     2881 cgtggtgtgc gggaagcgct aagcctcgat caccacttcg cgcaggcagg attcattctc
     2941 cttgtttgac gcacattcgc attcggcaac accgctcgca tgaggttggc gagattgccc
     3001 cgcgtttcat taccgcgtac cctctgcctg catgatcaaa gagctggatt gcgtggcgct
     3061 ggccgccgac cttcccgacc acgggcttgc cgtgggcgat gttggagcgg tcgttcatgt
     3121 ctatgaaggc ggccacagtt accagatcga attcacgacc tatgacggaa ggacggtcgc
     3181 tgtggcgaaa gtctccgccg atcagatccg ttccctcgga
//
LOCUS       DATCGS010000454         2989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_188483, whole genome shotgun sequence.
ACCESSION   DATCGS010000454 DATCGS010000000
VERSION     DATCGS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188483"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIL39_09335"
     CDS             complement(<1..809)
                     /locus_tag="VIL39_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY5481668.1"
                     /translation="MRHWSLILVAGWICGSPAGLPAADVGLIKVNGAIGPATASYIAR
                     AIDMAGERGDTCLIIQLDTPGGSLDSTKAIVQKFYASTVPTVVYVAPDGAWAGSAGCF
                     ITLAADIAAMAPGTSIGAAHPVSIGAGGEEKTSDVMKEKLEQITGSFIEGIAKRRGRN
                     IEWARSSVVESKAIDADKALELKVIDLIAKDLPDLLSQLDNREVGDKTLKTAGAEVVD
                     IPMTTSERILQVLSHPELMMILMLVAMYGIIGELSNPGAIFPGVVGAIALIL"
     gene            917..1387
                     /locus_tag="VIL39_09340"
     CDS             917..1387
                     /locus_tag="VIL39_09340"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY5481669.1"
                     /translation="MALVRILVDGYSLLHNWPELARGRARYSAAARDELIARLTLYQD
                     ASGIPVTIFFDGSGLSTGKPTPASLASVEVLYSRKGQTADQLIERAAYRYGPYGEVLA
                     VTDDHAERDTVISLGGSASSCWNFILTVENTLAELGEDIKHYNRRERHRFQRRK"
     gene            1410..2912
                     /locus_tag="VIL39_09345"
     CDS             1410..2912
                     /locus_tag="VIL39_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481670.1"
                     /translation="MDIGNPNMGKAEDRNKRRRTGFALTPSLLAGLLFIAALTPGPVL
                     AQADAGKAPLRSNRCLLVVETSRAMQRQSEAVRQIVQDMLRSGFGGQLRGGDTLGVWT
                     YNDRLYAGRFHLQTWSPEAQKDITQRVLTFLKDQKYEQQASFDKVLPALGRLVKDSPL
                     ITVILISSPDEKLRRTRYDYPINQAYENRKWREEQEKALMPLVTVLRAQDGQLGDYTA
                     NTPQERQRAETIQTKLLEAVRNAPPTAAPRVVLPAQERQLAEAVQAKLLEAVRNAPLT
                     NAAPVILPGKTTRLEKAPAPKPQPAGVNVQPPVPAPEAAIANKPLTVKPPVPAAQPVQ
                     IAKAEAAPVVPDKPSLDLPPKPSPSPKPVTEPEAEVAKAPETKPAAPASSKTEAAPAP
                     STPAPNPAPAAPAAREVSSGTIPNATAPASAPPDSSTLSLRPSPLPVPPVQTATAIPA
                     ETLARNRSIWIAGLVLAGAAISLALLLLRRSRATAGGSLITQSFERKHKP"
BASE COUNT          569 a         1007 c          896 g          517 t
ORIGIN      
        1 agaatgagcg caatggcgcc caccaccccc ggaaatatcg cccccgggtt gctcagttcc
       61 ccgatgatcc cgtacatcgc cacgagcatg agaatcatca tcagttcggg gtgtgagagg
      121 acttgaagga tacgctccga cgtggtcatg ggaatgtcca ccacttccgc gccggccgtc
      181 ttgagagtct tgtcgccgac ctcgcggttg tcgagctggc tgagcaggtc cggcaggtcc
      241 ttggcgatga ggtcaatgac cttcaactcc agcgccttgt cggcatctat cgccttgctc
      301 tcgaccaccg aagacctggc ccactcgata ttgcgcccgc gccttttggc gatgccttcg
      361 atgaagctgc cagtgatttg ctccagcttc tccttcatca cgtcgctggt cttctcctcg
      421 ccgccggccc cgatggaaac cgggtgcgcc gcgccgatgc tcgtgccggg ggccatggcg
      481 gcaatgtccg ccgccagcgt gatgaagcat cccgcgctgc cggcccaggc cccgtcggga
      541 gccacataca cgaccgttgg caccgtggag gcgtagaact tttggacaat ggccttggtg
      601 gaatcgagcg agccgccggg agtgtcgagc tggatgatca ggcaggtatc gccgcgttcg
      661 ccggccatat caatggcgcg ggcgatgtag ctggcggtgg ccggtccaat ggcgccgttg
      721 accttgatca ggccgacatc cgcggctggc agacccgcgg gcgacccaca gatccacccc
      781 gccaccaaga ttagcgacca gtgcctcatg gattccttaa tatgctttac aataacagaa
      841 tcggcagagt gcgcaaatgc tattcgagag gcggttttgc attggccaac ccgggaccgg
      901 gacattatgg ttgcgtatgg ccctggtgcg gattctggtc gatggctaca gcctgctgca
      961 caactggccc gagctggctc gcggccgggc gcggtattcc gccgccgcgc gcgacgaact
     1021 gatcgcccgg cttacgcttt atcaggacgc gagcggcatt ccagtcacga tcttctttga
     1081 cggctccggg ctgtcaactg gcaagccaac ccctgcctct ttggcgtctg tcgaagtcct
     1141 ctactcgcgg aagggccaga cggccgacca gttgatcgag cgcgcggctt accggtacgg
     1201 cccctatggc gaggtcctgg ccgtcacgga cgatcatgcc gaacgagata cggtgatcag
     1261 cctgggcggc agcgcgtcca gttgctggaa cttcatcctg accgtcgaaa acaccttggc
     1321 ggaactgggc gaggatatta aacattacaa ccggcgagag cggcacagat ttcagcggcg
     1381 gaagtgaaga gccggcacca agcgagcaag tggacatagg caatccaaac atgggcaagg
     1441 ccgaagaccg caacaagcgg aggcgcacgg gttttgcgct gacgccgtcg ttgctggcgg
     1501 ggctgctttt catcgcggcg ctcactccgg gcccggtcct ggcccaggct gatgccggca
     1561 aagccccgct gcgctccaac cgctgcctgc tggtggtgga aacctcccgc gccatgcaac
     1621 gccagtcgga ggccgtgcgg cagatcgtgc aggacatgct gcggtccggc ttcggcgggc
     1681 agttgcgcgg cggggatacg ctgggggtct ggacttacaa cgaccgcctt tacgcgggcc
     1741 gcttccactt gcagacgtgg tcacccgagg cgcagaagga cattacccag cgcgtgctca
     1801 ctttcctcaa agaccagaag tatgagcagc aggccagctt cgacaaagtt ctgcccgcgc
     1861 taggccgcct cgttaaggac tccccgctta tcacggtcat cttgatttcc agtcctgacg
     1921 agaagctgcg ccgcacgcgc tacgactatc cgatcaacca ggcgtacgag aaccggaagt
     1981 ggcgtgagga gcaggagaaa gccctgatgc cgctggtcac tgtcttgcgg gcgcaggacg
     2041 gccagcttgg cgactatact gcgaacacgc cccaggagag gcaacgcgcg gaaacgattc
     2101 aaaccaagct cctggaagcc gtgcgcaatg ccccacccac agccgcaccg cgggtggtcc
     2161 ttcccgctca ggagaggcaa ctcgcggaag cggttcaagc caagctcctg gaagccgtgc
     2221 gcaacgcccc actcacaaac gcagcgccgg tgatccttcc cgggaagacg acccgactgg
     2281 agaaagcgcc ggcgccaaag ccgcaaccgg ctggtgtcaa cgtccagccg cccgtccctg
     2341 caccggaggc tgctattgct aacaagccgc tcacggtcaa accgcccgtg ccagcggcgc
     2401 aaccagtcca aatcgccaaa gccgaagctg caccggttgt gcctgacaag ccttcgttgg
     2461 atttgccgcc aaagccttca ccgtcgccca agccggtcac ggaacccgag gcggaagttg
     2521 cgaaggcccc cgagacaaaa ccagccgcac ccgcatcttc aaagaccgag gcggctccgg
     2581 caccctcgac tcccgcgcct aacccagctc cggcggcccc cgccgcccga gaagtttcat
     2641 ccggaaccat tcccaatgcc accgcacctg cgtccgcgcc acccgactcc tcgactctct
     2701 ctctccgtcc ctcgcctctt cctgtcccgc ctgtccaaac ggccactgcc attccggccg
     2761 agaccttggc ccgcaaccgg agcatttgga ttgcaggact cgtcctggcc ggggcggcga
     2821 tcagcctcgc gttgctgctg ttgcgccggt cgcgcgctac agcagggggg agcttgatta
     2881 cccaatcatt cgagcgtaag cataagccat gaaccgacgc aagtttctcc aaaccgcggg
     2941 cgcattggcc gtggcggcca ccgccgcgcc gatgtccccg ctgcacgcc
//
LOCUS       DATCGS010000455         7535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_335866, whole genome shotgun sequence.
ACCESSION   DATCGS010000455 DATCGS010000000
VERSION     DATCGS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335866"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..785)
                     /locus_tag="VIL39_09350"
     CDS             complement(153..785)
                     /locus_tag="VIL39_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481671.1"
                     /translation="MTEWHIQSRAHACSACGKAFADKEVYHTLLFDQKADFQRSDICE
                     ACWQKQYSEGARDRKGFVSYWHGVYVAPTPLVEAIQKENAESLLRKLIELNDPRYIPA
                     GYILAVMLERKRLLKIKEQITRDGHRVFIYEQPGTGDVFTIIDPDLQLNQLEQVQHDV
                     GALLEHGLNPPAPPVPATEAEAAPAAAPADAPAPAAEPGAQAAPEEPKAA"
     gene            complement(793..3981)
                     /locus_tag="VIL39_09355"
     CDS             complement(793..3981)
                     /locus_tag="VIL39_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY5481672.1"
                     /translation="MNVFMFRSQRSSSFSATAALILIGIAPSGWSQIGNGARRILISL
                     IIAGAAMLAFLVLVSSATAAAPPEYRAFWVDGWGAGFLRQSQVDTLLGIPGTISTGQL
                     RDANCNAVFIQVRRNCDACYPSSMGEPYMSGLSPANFNALQAVINAAHDTTGGKKRIE
                     VHCWMVTFRTSGGAVYAQHDDTPTGSLTTLDNYWPSRDNTGAEVSDKAFDPGHPLALQ
                     YTVNVALDLVNNFDIDGIHYDYIRFTANNQGYNPTSIARYNARYGLTGQPSAANEQFK
                     QWRRDQVSAVVRQAYAKIQKSKPQVKQSGSFVTWNPSPTSSTRAGFQATRPYYDVYSD
                     WDSWLQEGIVDMAVPMTYYDWASLPNDYTRWINFEKDRRGDRHMIIGPGIYLNSLANA
                     VYVIQLTRDASPAANHAQGFSGYSYGVPYVNGTWAGFSPSLVSQVTPTWADIPDMPWK
                     SAPTKGHLMGAVTIADSGAWADGATVSITGPVNRSLDVDGTGFYAFIDLPPGGYNLTV
                     SKLGYLEAPATVNVAVGEVTGNMYEQDFVLGPTALATIIDQPQDQTVSQGDPATFTVT
                     ANGTPPLYYQWRFYGTNLVGATDSSYTRANAQLADAGLYSVIVSNAFNTAISSNALLA
                     VVVFPVISSQPRSVAVAPDQGATFSVTAAGTPPLLYQWRFNGAALAGQTAATLTVPFA
                     RTTNAGAYSVLVSNLYGVTLSSDALLALLPAAAGDNSFGLGQVPASAANTIAVAAGAW
                     HNLALRADGAVVAWGDASRGQCEVPAALADVVAIAAGGYHSLALRMDGTVVAWGANDY
                     GQIGVPAGLGNVIAIAAGTWHSAALRANGTVAVWGDNNFGQRSVPAGLTNVTAIAAGG
                     NHTLALKADGTVVAWGENTDAEGILAGQSDVPSGLTTVKAIAAGEYHSLAVRGDGTVV
                     AWGENSQGQCSVPPALANVVAVAGGGSHSVALGTDGAVTAWGSDWSGQCEISSSFCPA
                     SSMTAGSYHTVVVPEGTLPVPRLMNPARQGSRFNTLVQTLSRKSYVLECKPSLDATNW
                     TALSTNAGNGALKILTDPAASGRQRFYRLRQW"
     gene            4126..5520
                     /locus_tag="VIL39_09360"
     CDS             4126..5520
                     /locus_tag="VIL39_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5481673.1"
                     /translation="MKMTLCSDPNRCALALGAAAVCALLSLLAPARAAEGAKPNVIII
                     LADDQGSVDAGCYGAKDLVTPGVDALAARGVRFTQFYSAAPVCSPSRAGLLTGRYPWL
                     AGMPNNGAAPPKEADDQLDTLTGEGLPARERTLADMFRGAGYATAHIGKWHLGSGAGH
                     KPLDHGFDYSFGFMGGCIDNYTHFFYWAGPNRHDLWENNQRVRLPGQYFPDLMVEKAK
                     AFIGQHRERPFFMYFAINMPHYPYQGDEKWLQYYRRLPYPRRLYAAFVSTLDERVQRL
                     MQYLAEAGLRERTIIVYQSDNGHSVEERAHFGGGSSGPYRGAKFSLFEGGIRLPAIIS
                     WPGHLPEGQVRGQVAHGCDWMPTLAELCGVELPKAELTGRSLVPVLQSANAPSPHDVL
                     QWRLGNQWAVRQGPWKLLHNPNDQADAQKLDPPDREWFLANVDEDVGERTNLAPRHAE
                     IVQRLRKLAPVGTE"
     gene            5620..7242
                     /locus_tag="VIL39_09365"
     CDS             5620..7242
                     /locus_tag="VIL39_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEY5481674.1"
                     /translation="MNDTGFSRRQALGRLGLMAAGAAAATPLGASAEEVKATAAGMAY
                     NVRDFKAVGDGRAKDTAAIQKAIDAASAAGGGMVLVPNGLYLCGTLQLRDNVTLHLAE
                     GATILGSTEGSDYLNIAASGFNRAPLALVLALKARNIGITGRGTINGQGGSFSHKDNA
                     PGRPFGIKLVDCQDVLIADVRVQDAAAWVIHLLRCERAEVRGVRVWSHANYNNDGLDI
                     DACADVAVSQCHFDCQDDAICLKSTPDRICENIVISDCIASSHCNLIKMGTSSVGGFR
                     NVTITNCVLVAPRYSKKVNGADRGIGGINLELVDGGVMDRITISNITMDGITLPLFIR
                     LGSRKRKDNQPASTLRNVMISNVVATNLGVVGSSITGLPGQRIENVTLSNLQFTYEGG
                     GTAEDAQRAVPERANAYPEGWMFGTLPAWGLYCRHVKGLKLHNVKLLLAAPDARHAVV
                     FDDVEELDLDGLDCACSPGAAALVKMTGTRAATIRGCRPQSPKGVFLKLEGQENAGIA
                     LLANDLRQAGKWLEADPDAARDAVAQAGNLVA"
BASE COUNT         1365 a         2291 c         2527 g         1352 t
ORIGIN      
        1 atgggcgcgc cttgctcgtg agcgaccgag gggtgcgtca aggcgagccg cagcagagcc
       61 gggtcgcgga agacataacc cagccgatct tgcagcgtat cgtattcggt gacagcgggc
      121 atggggcggt ctttcagatg caggaattca gttcaggcgg ctttcggctc ttcgggcgcg
      181 gcttgggctc cgggctctgc cgcgggcgcg ggcgcgtcgg caggggcggc ggcgggcgca
      241 gcctcagcct cggttgccgg cactggcggg gccggcgggt tcaagccgtg ttcgagcagg
      301 gcgccaacgt cgtgctggac ttgttcgagc tggttgagct gtaagtccgg gtcaatgatg
      361 gtgaacacgt cgcccgtgcc aggttgttca tagataaaaa cgcggtgccc atcgcgcgtg
      421 atttgctctt tgatcttgag cagacgcttg cgttcgagca tgacggcgag gatatatccg
      481 gccgggatgt agcgcgggtc gttcagctcg atgagcttgc gcaggagcga ctcggcgttc
      541 tccttttgaa tcgcctcgac gaggggcgtg ggcgcgacat agacgccgtg ccaataggag
      601 acgaagcctt tgcggtcgcg cgcgccctcg ctgtattgct tttgccagca ggcctcacag
      661 atatcggagc gctggaaatc ggccttctga tcaaagagca gggtgtgata cacttccttg
      721 tcggcaaagg ctttgccgca ggcggagcaa gcgtgggcgc gtgattggat gtgccactca
      781 gtcatattac cgttaccatt gccgcaggcg gtagaaccgc tgcctgccgg aagccgccgg
      841 atcggtgaga attttcagcg cgccgttgcc ggcgttggtg ctcagcgcgg tccagttggt
      901 tgcgtccagg gaaggcttgc attccagcac gtaactcttc cggctcaagg tttgcaccag
      961 cgtgttgaag cggctgcctt gcctggccgg attcatcagc cggggcaccg gcaaggtgcc
     1021 ttccgggacg accaccgtat gataactgcc ggcggtcatt gacgacgccg gacagaagct
     1081 ggacgagatt tcgcactggc cgctccagtc agaaccccag gcggtgacgg cgccgtccgt
     1141 gcccagcgcc acgctgtgtg atccgccgcc cgctaccgct accacattgg ccagcgctgg
     1201 cggcacgctg cactggccct gcgagttctc gccccaggcc acgacggtgc catcgcccct
     1261 caccgccagg ctgtgatatt ccccggccgc aatggccttc acggtcgtca accccgaagg
     1321 cacgtccgat tgtcccgcga ggattccttc cgcatcggta ttttctcccc aggccacgac
     1381 ggtgccgtcg gccttcaagg ccagggtatg attgccgccc gccgcgatgg cggtcacgtt
     1441 ggtcaacccg gcaggcacgc tacgttgccc gaagttgttg tccccccaga cggccacggt
     1501 gccattggcc cgcagcgccg cgctgtgcca ggtcccggcc gcaatcgcaa tgacgttgcc
     1561 caagcctgcc gggacgccaa tctgaccgta atcgttggcc ccccaggcca ccaccgtgcc
     1621 gtccatccgg agcgccaggc tgtgatagcc accagcggca atggccacga cgtccgcgag
     1681 cgccgcaggc acctcgcatt gtccgcgtga tgcatcgccc caggcgacca ccgcgccgtc
     1741 ggcacgcaag gccaggttgt gccaggcccc cgcggcgacg gcgatagtgt tcgcggccga
     1801 cgccgggacc tggcctagac caaagctatt atcccccgcc gccgccggca ggagtgccag
     1861 cagcgcgtcc gagctgaggg tgacgccata gaggttgctc accaggacgg agtaggcgcc
     1921 cgcattggtg gtgcgcgcga atggaaccgt gagcgtggcc gcggtctggc ccgccagggc
     1981 ggctccgttg aatcgccact ggtaaagcag cggaggcgtg ccggctgcgg tcaccgagaa
     2041 cgtcgcaccc tggtcgggag caactgccac actccgcggt tggctggaga tgaccgggaa
     2101 cacaaccacc gcgagcagcg cattggagct gatggccgtg ttgaaggcgt tggaaacgat
     2161 gacggagtac aacccggcat ccgccaactg ggcattggcg cgggtgtagc tggagtccgt
     2221 ggcgccgacc aggttggtgc cataaaaccg ccattgataa taaagcgggg gcgtgccatt
     2281 ggcggtgacg gtgaacgtcg cggggtcgcc ctggctgacg gtctggtcct ggggttgatc
     2341 tatgattgtg gccaaggcgg tggggcccag aacaaagtcc tgctcgtaca tgttgccggt
     2401 aacctcgccg accgccacgt taactgttgc cggcgcctcg aggtagccga gcttgctgac
     2461 ggtcaggttg tagccgccgg gcggcagatc aatgaaggca tagaagcccg tgccatccac
     2521 gtccaggctg cggttgaccg ggccggtgat gctcacggtg gcgccgtccg cccaggcgcc
     2581 cgagtcggcg atggtgaccg cgcccatcag gtggcccttt gtcggcgccg atttccaggg
     2641 catgtcggga atgtcagccc acgtcggcgt gacctgcgaa accagcgagg ggctgaaccc
     2701 ggcccaggtg ccgttcacat aaggcacccc gtaggagtag ccgctgaacc cctgggcatg
     2761 gttggcggcc ggcgacgcgt cacgcgtcaa ctggattacg taaacggcgt tggctaacga
     2821 gttcaggtag atgccgggcc cgatgatcat gtgccggtca ccgcgccggt ccttctcgaa
     2881 gtttatccag cgcgtgtagt cgttcggcag gctggcccag tcgtagtagg tcatcgggac
     2941 cgccatgtcc acaatcccct cttgcagcca gctatcccag tcggagtaca cgtcatagta
     3001 aggacgcgtg gcctggaagc ccgcccgggt cgaggaggtg ggcgaggggt tccaggtgac
     3061 gaaagagccg gactgcttga cctggggctt gctcttctga atcttcgcat aggcctgccg
     3121 cactaccgcg ctgacctggt cgcgccgcca ttgcttgaac tgctcgttgg ctgcggaggg
     3181 ctggccggtc agcccgtaac gggcgttgta gcgggcgatg ctggtcgggt tatagccctg
     3241 gttgttggcc gtgaaacgga tgtagtcgta gtggatgccg tcaatgtcga agttgttcac
     3301 caggtccagg gccacgttga cggtgtactg gagggcgagc ggatggccag gatcgaaggc
     3361 cttgtcgctg acctcggcgc cggtgttatc gcggctcggc cagtagttgt ccagggtcgt
     3421 caagctgccc gtcggggtgt cgtcatgctg ggcatagacc gcgccgccgc tggtgcggaa
     3481 ggttaccatc cagcagtgga cctcgatgcg cttcttgccg cccgtggtgt cgtgggcggc
     3541 gttgatcacg gcctgcaagg cgttaaaatt ggcgggggag aggccggaca tgtagggctc
     3601 gcccatgctg gagggatagc aggcgtcgca attccggcgc acctggatga agaccgcgtt
     3661 gcagttggca tcgcggagct gacccgtgga aatcgtgccg ggaatgccaa gcaaggtatc
     3721 cacctggctt tgtctcagga aaccagctcc ccagccgtcc acccagaacg cgcggtattc
     3781 aggcggagca gccgcagtcg cggaggaaac cagaacgaga aaggcgagca tggccgcgcc
     3841 ggcaatgatc aaggagatca gtatgcgccg cgcgccgttg ccgatctggc tccagccgga
     3901 cggggcgatc ccgatgagga tcagggcggc agtcgccgaa aaagaggatg aacgctggga
     3961 gcggaacata aaaacgttca taagtatttg ccgatccgct gagggtgtca atctgaatgg
     4021 gtaagccaat tcggcccaag gcgcatcttc ctggttccac cggagtgcgc ccgtccgtat
     4081 attgagtctg gcagcgctgc cagagatacg ccaccctgga gaccaatgaa gatgactctt
     4141 tgttcagacc cgaaccgatg tgcccttgcc cttggcgcag ccgcggtgtg tgcgctgctc
     4201 agcctgctcg cgcctgcccg agctgcggag ggggcaaagc ccaatgtcat catcatactc
     4261 gcggatgatc agggcagcgt ggacgctggc tgttacggcg ccaaagacct cgttaccccc
     4321 ggagtggatg ccctggcggc ccgcggggtg cggttcacgc agttctattc ggcggcgccg
     4381 gtctgttcgc cctcgcgggc gggcttgctg acgggtcgct atccgtggct ggccgggatg
     4441 cccaataacg gcgccgcgcc gccgaaagag gccgacgacc aactggacac acttacgggt
     4501 gaaggcttgc ccgccaggga gcgcacgttg gcggatatgt tccggggcgc aggctatgcg
     4561 acagcacata tcgggaagtg gcatcttggg tccggtgccg gccacaagcc gctcgatcac
     4621 ggtttcgact attcgtttgg cttcatgggc gggtgcattg acaactacac gcacttcttt
     4681 tactgggccg gccccaaccg tcacgacctt tgggaaaaca accagcgggt gcggttgccg
     4741 gggcagtatt tcccggacct gatggtggag aaggccaagg cgttcatcgg gcagcaccgg
     4801 gagcggccgt tcttcatgta tttcgccatc aacatgccgc actatcctta ccagggagat
     4861 gagaagtggc tgcaatacta tcggcgcctg ccgtatccgc gccggcttta cgccgccttc
     4921 gtctccacct tggacgagcg ggtgcagcga ctgatgcagt atcttgcgga ggccggtttg
     4981 cgggaacgga ccatcattgt gtatcaatcc gacaacggcc attcggttga ggaacgagcc
     5041 catttcggcg gcggcagcag cggtccgtat cgcggcgcga agttcagctt gtttgagggt
     5101 ggcattcggc tgccggccat catctcctgg ccaggccatc tccccgaggg acaggtccgg
     5161 ggacaggtcg cccatggctg cgactggatg cccacgctgg cggagctatg cggcgtggaa
     5221 ctccccaagg cggagttgac cgggcgcagc ctggtgccgg tgcttcagtc ggccaatgct
     5281 cccagcccgc acgatgtgct gcaatggcgg cttggcaacc agtgggccgt gcgccagggc
     5341 ccctggaagc tgctccacaa tccaaacgat caagccgacg cccaaaagct tgaccccccg
     5401 gaccgggaat ggttcctggc caacgtggat gaggatgtgg gcgaacgaac gaatctggcc
     5461 ccgcgccacg cggagatagt ccagcgtctg cgcaaactgg cgccggtggg aacagagtga
     5521 gctgcgcaag ggtttcggcg ttgaccccga agcgagagtc gagcaggctc gcgcgacagt
     5581 gaaccagcaa acacgttatt cgaaggaggc ggcagaacca tgaacgacac tgggttttcg
     5641 cgcaggcagg ccctcgggcg actgggtttg atggccgcgg gggcggcagc ggcaacaccg
     5701 ctcggggcca gcgccgaaga ggtgaaagct acggcggcag gcatggctta caatgtgcgc
     5761 gattttaaag ccgtcggcga cgggcgggcg aaagacacgg ccgctatcca gaaggcgatc
     5821 gacgcagcca gtgcggcggg cggcgggatg gtcttggtac ccaatggcct ctatctctgc
     5881 ggcacccttc agcttcgcga caacgtcacg ctccacctgg cggagggagc gacgatcctg
     5941 gggagcacgg aaggcagcga ctatctgaac attgcggcca gcggtttcaa ccgggccccg
     6001 ttggcgctgg tcctggcgct aaaggcgcgc aacatcggca tcacgggccg gggaaccatt
     6061 aacggacagg gcggcagctt ctcccacaaa gataatgcgc ccggccggcc gttcggcatc
     6121 aagctggtgg attgccagga tgtgctcatc gcggacgtgc gcgtgcagga cgcggcggcc
     6181 tgggtcattc acttgctccg ctgcgagcga gccgaggtgc ggggcgtgcg cgtctggagt
     6241 catgccaatt acaataacga cggcctcgac atagacgcct gcgcggacgt agccgtttcc
     6301 cagtgtcact tcgattgcca ggacgacgcg atctgcctca agagcacccc ggaccggatc
     6361 tgcgagaaca ttgtgatcag cgactgcatc gccagcagcc attgcaacct catcaagatg
     6421 gggaccagct ccgttggcgg attccgtaac gtaaccatca ccaattgcgt gctggtggcg
     6481 ccacgctatt ccaagaaggt gaacggcgcg gaccgcggca tcggcggcat caatttggaa
     6541 ctcgttgatg ggggcgtcat ggaccggatc accatctcga atatcacgat ggatgggatc
     6601 acgctgccgt tgttcatccg tctggggagt cgcaaacgca aggacaacca gcccgccagc
     6661 accctgcgca atgtcatgat cagcaatgtg gtggcgacga acctgggcgt ggttggcagt
     6721 tccatcacgg gcctgcccgg ccagaggatc gagaatgtca cgctgagcaa cctccaattc
     6781 acctatgaag gcggaggcac ggcggaggat gcgcagcgcg ccgtccccga acgtgcaaac
     6841 gcctatcccg aaggttggat gtttggcacg ctgccggcct ggggtctgta ctgccgccat
     6901 gtcaaaggac ttaagctgca caacgtgaag ctgctcctgg ccgcgccgga cgcccgccac
     6961 gccgtggtct tcgacgacgt ggaggagctg gacctggatg ggcttgattg cgcctgctcg
     7021 ccgggggccg cagcgctggt gaagatgacc gggacccgcg ccgccacgat ccgaggctgc
     7081 cgtccccagt cgcccaaggg cgtgtttctc aagctcgaag gccaggaaaa tgccgggatt
     7141 gcgctgctgg cgaatgatct gcggcaagcc ggcaagtggc ttgaagccga cccggacgcg
     7201 gccagggacg ctgtggccca ggcggggaat ctagtggcgt aagacgcctg gccctgacgg
     7261 aatcacgcct ccggttttgg acactaccgt ccggagcaat gacagcaatt gcagggacgt
     7321 ttcgccgaaa cgtccgtgtt tgcgcgagtg cggacacctc ggcgaggcgt ccctaccaaa
     7381 caagcgcgcc taaacgcgag cttgatgcag ccctgggccc ccaaaccctg ctcaaacccg
     7441 ggcttgacgt ttccgaatta gctgccatat tctccggcct gattccaaac cggaggttgt
     7501 agttgcagcc ccgcagatgt ggggcctcat cgcgc
//
LOCUS       DATCGS010000456         5211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_188528, whole genome shotgun sequence.
ACCESSION   DATCGS010000456 DATCGS010000000
VERSION     DATCGS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188528"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VIL39_09370"
     CDS             <1..303
                     /locus_tag="VIL39_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY5481675.1"
                     /translation="LPLGGNVIHKRFTPPTRKAVSDVLTASIQYSLDHRPEAVQHALQ
                     YARDMGRDLADKFVGMYVNHWTLDYGEKGRESIRRFLSRGFEMGVIPQQPELEFVG"
     gene            complement(313..528)
                     /gene="infA"
                     /locus_tag="VIL39_09375"
     CDS             complement(313..528)
                     /gene="infA"
                     /locus_tag="VIL39_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5481676.1"
                     /translation="MKEEHIEVEGRVQTVLPGTMFRVELDNKHLVLATICGKMRKRWV
                     RLTVGDRVKMEMSPYDLDKARIVWRLR"
     gene            complement(657..998)
                     /locus_tag="VIL39_09380"
     CDS             complement(657..998)
                     /locus_tag="VIL39_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY5481677.1"
                     /translation="MSAKLFVGNLSFNITENDLQDAFAAHGTVVEANLMMDRATGRPR
                     GFAFVTMSTPEEAQKAIDALNGKELGGRALTVNIARPREERPGGGGGGGGRGGYGGGG
                     RGGGGGGRDRY"
     gene            1326..1934
                     /locus_tag="VIL39_09385"
     CDS             1326..1934
                     /locus_tag="VIL39_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5481678.1"
                     /translation="MTESGYREAIIEYIRAQARPPDKFSHQPRLYRLAVRLAEGRPYD
                     DDVLYGAAWMHDLGVFVGHRPENPAALAAWDHVAYAARQVPSVLRQFGFPEGKIAAAI
                     EIIRTHLPAAKPTSFEGTLLRDADILEQLGAIAILRTVSKVGRDTRFVRYSDAVRVLR
                     RNAEELPAQLELASARRLAAPRVEILKAFLAAAEAEADGNEL"
     gene            1980..2783
                     /gene="thiM"
                     /locus_tag="VIL39_09390"
     CDS             1980..2783
                     /gene="thiM"
                     /locus_tag="VIL39_09390"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEY5481679.1"
                     /translation="MKPTAYDLLDRVRQQKPVVHHLTNWVTIYDCAQVVKTLGASPVM
                     AHAPEEAAEMAQIASALVLNIGTLTVDFVEAMKLAAQSANKKGIPVVLDACGAGATAL
                     RDRKCLELLNEVRIDVIKGNASEVARVSGESVKTRGVDATEVSGDLVLLAEKLAAQRK
                     ATVVITGKVDITTDGRQTHLVKNGHPLMARVVGTGCMAASVIGAFAAVESDLSLAAAC
                     ALAIYGIAGEVAAEKASGPASFKERLFDCLYNLDRESVDRMQRIETSQR"
     gene            complement(2804..3556)
                     /locus_tag="VIL39_09395"
     CDS             complement(2804..3556)
                     /locus_tag="VIL39_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481680.1"
                     /translation="MTSEHTTPSTPALGGIFVTTQWTQVLATRGDSPEARQALSDLCA
                     AYYTPVFVMIRRAAPNEEAARDLTQEFFARLLARAGLGQVDPERGRFRFFLLGAVTHF
                     LADAHDEQCRLKRGGGRAPISLDASTDSSSGLEIADTSRPSPELEFDREWAVTLLDRA
                     LERLGREQAAVGNGEQFPALKPWLTGDKQEVSLADLAARLGTSEGALRVALHRLRKRF
                     RELVREEIAGTVGDATHVREEMGYLLEVLSRG"
     gene            complement(3553..>5211)
                     /locus_tag="VIL39_09400"
     CDS             complement(3553..>5211)
                     /locus_tag="VIL39_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481681.1"
                     /translation="QTGDFPGKLPLTLFFKTGVTKLGLLQITGFTENPRGVKIRYKLV
                     LPQSANLSARLAHVNHRNTNTAGFGPVIERTINLSEPERDMFLSFETGKLMKEPGGRL
                     NTPEPEKLRVADEALTWARSSGADVLIRFCVGKTGPDGSIEVPQLELRSLDMAMFPTN
                     TESWDGLSSQTIATAVRSAPEHGWWQGLAWGTRDFTFLFRTRQGNAGILLVTEYTDKP
                     RSVKLRYKLVQTSRNSLPEGTGKPEAQPANRMVLQAAEPMSSVASTATTFSVTAAGTA
                     PLAYQWWHGPTNSAELSPATTGSAASHGLPFVARLAQGEVELLALSHHPSTNQTWWRP
                     DGTPWTNVVFHNPSTSSSPTKGQRFEFVFQRRGLPPDTTLECSFEPGTGEVSMGSRPW
                     RQGKELPDHNVIEAVLPEPAKEVTIRLGVAAGEWKTMLARTPQAGAGSSFTLGDQTCR
                     ALFLDPVESSGEARVGVSYNKVPGWTTRVTALDTKGQLHVSFPYENSIDDVASAEGRF
                     PGLPLSRVKKFRFEARPFTGVEFRNVSLQPGEHTQVEVVNVGDK"
BASE COUNT          977 a         1632 c         1583 g         1019 t
ORIGIN      
        1 ctgccgctcg ggggcaacgt gattcacaag cgcttcaccc cgcccacgcg caaggcggtg
       61 tccgacgtgt tgaccgcgag catccaatac agcctcgatc accgcccgga agctgtgcag
      121 cacgccctgc aatatgcccg cgacatgggc cgcgacctgg cggacaagtt cgtggggatg
      181 tatgtgaacc attggacgct ggactacggc gagaagggcc gcgagtctat tcgacgtttt
      241 ctgagccggg gctttgagat gggcgtaatc ccccagcagc cggaattgga gttcgtgggc
      301 tgaccttgga ttctacctga gccgccagac gatgcgcgcc ttgtccagat cgtagggcga
      361 catctccatc ttaacgcgat caccgaccgt tagtcggacc cagcgtttgc gcattttgcc
      421 gcagatagtg gccaggacga ggtgcttatt gtcgagttct acccggaaca tcgtgcccgg
      481 gagcacggtt tggacgcgcc cttcgacttc aatgtgttct tctttcatag tctgcttggc
      541 cgacggctgg gtctcctggc acaggcgaca ccccgcagac cggccaaaaa gggtggcgcc
      601 aaagaaaaaa aggaggcccg gtacgcccgg gccccgtgcg aaaataaact gctgaattag
      661 tagcgatcgc gaccgccgcc accaccgccg cggccaccgc caccgtaacc accgcgaccg
      721 ccgccgccgc cgccgccgcc cggacgctct tcgcgaggcc gagcaatgtt aactgtcagg
      781 gcgcggccgc ccagttcctt gccgttgagc gcgtcgatgg ccttctgggc ctcttccggc
      841 gtgctcatgg taacgaaggc aaacccgcgg ggacggccgg ttgcccggtc catcatcagg
      901 tttgcttcga cgaccgtgcc gtgcgccgcg aaggcgtcct gcaggtcatt ttcagtgatg
      961 ttgaaggaaa gattcccaac aaacaatttt gcgctcatgt gatgtaacta actatactgt
     1021 cttagactaa cttttgcttt ctccagactg ttcccgctta tcgggtttca aatcatcact
     1081 gaaccgagtt cacctgaaga tttaccatgc catggaattg acaagctatc taacagacga
     1141 gcgaatactg accttttttg gctggatagc aagctttttt ttgctttaat tttggacagg
     1201 ggggcgtctc cggctttgtg catccgcaag ccgtcttcca ttcacacccg ctaaagcgat
     1261 gtgttaatgc aagttctggg aactcgcgga ttggaaatca acggcgttgc tgctattctg
     1321 gcgccatgac tgaatccgga taccgggaag ccattattga atacatccgc gcccaggcga
     1381 ggccgccgga caagttcagc caccagcccc ggctttatcg cctcgcggtg cggctggcgg
     1441 aaggtcggcc ttacgatgac gacgtcctct atggcgccgc ctggatgcac gacctcgggg
     1501 tattcgtcgg ccaccgtccg gaaaacccgg cggcgctcgc cgcgtgggac catgtggctt
     1561 atgccgcccg ccaggtgccc tcggtcctga ggcaattcgg gtttcccgaa ggcaagattg
     1621 cagccgccat tgaaatcatt cggacacatc tgcccgcggc caagccgacc tcgtttgagg
     1681 gcacgttgct gcgggatgcg gatatcttgg aacaactcgg ggccatcgcc atccttcgca
     1741 cggtcagcaa agtgggccgc gacacgcggt ttgtgcggta ttcagacgcg gtccgggttc
     1801 tgcggcggaa tgcggaggaa ctgcccgcgc aactggagtt ggcctccgcg cgccgtctcg
     1861 ccgcgccgcg ggtcgagatc ctgaaggctt ttctggcagc cgccgaagcc gaggcagacg
     1921 gaaatgagtt gtaacccgag gccgagactg aacagcatgt cctccattcc agcactccca
     1981 tgaaaccaac agcttacgat ttattggaca gagttcgtca gcaaaagccc gtggtgcatc
     2041 acctgaccaa ctgggtgacg atttacgatt gcgcccaggt ggtcaaaacc ctgggcgcat
     2101 caccggtaat ggcccacgcc ccggaggagg ccgccgaaat ggcccagatc gcctccgccc
     2161 tcgtgctgaa tatcggcacc ctgacggttg attttgtgga agcgatgaag ctcgcggccc
     2221 agagcgccaa caaaaaaggc attccagtgg tcctggacgc ctgtggcgcg ggcgcaaccg
     2281 ccttgcgtga ccggaagtgc ctggagctgc tcaacgaggt caggattgat gtgattaagg
     2341 gcaacgcttc tgaagtggcc agggtcagcg gagaaagcgt caaaacgcgg ggagttgatg
     2401 ccacggaggt cagcggtgac ctcgtcttgc tggcggagaa actggctgcc cagcgcaagg
     2461 ctacggtggt aattaccggc aaagtggata tcactaccga tggccggcag acgcatctcg
     2521 tgaagaacgg ccatccgctg atggcgcgcg tggtggggac cggctgcatg gcggcctcgg
     2581 tcattggcgc ctttgcggca gtggaatccg acctgagcct ggcggcagcg tgtgccctcg
     2641 cgatttacgg catcgcagga gaagttgccg cggagaaggc ttccggccct gcgtcgttca
     2701 aggagcggct attcgactgc ctctacaacc tggaccggga aagcgtggac cggatgcaaa
     2761 gaatcgagac atcccagcgg taagctaaag ccggctcgcg gtctcacccc cgcgaaagca
     2821 cttccagcag gtagcccatc tcctcccgca cgtgggttgc atccccgacg gtgccggcga
     2881 tctcctccct gaccaactcc cggaatcgct ttcgcaggcg atgcaatgcc acgcgcagcg
     2941 ccccttcgct cgtaccgagc ctggctgcca ggtccgccag cgaaacctcc tgtttgtctc
     3001 ccgtgagcca cggcttgagt gccgggaatt gctccccgtt ccccaccgct gcctgctcgc
     3061 gcccaagccg ttccagcgcc cggtcgagca gcgtcaccgc ccattcgcgg tcaaactcca
     3121 gctcggggct cggacgactc gtatccgcaa tctccaggcc ggaagaactg tccgtgctcg
     3181 cgtcgagcga gatgggcgcc cgtccgccgc cccgtttcaa acggcactgc tcgtcgtggg
     3241 catccgccag gaaatgcgtc accgccccca gcaggaaaaa gcgaaagcgc ccgcgctccg
     3301 gatcgacctg ccccagtccc gcccgcgcga gcagccgggc gaagaattcc tgcgtcaggt
     3361 cgcgggccgc ttcctcgttc ggcgccgccc ggcgtatcat gacaaagacc ggcgtgtaat
     3421 acgctgcgca caagtcgctc aacgcctgcc gcgcctccgg cgagtctccc cgggtcgcca
     3481 gcacctgcgt ccattgcgtg gtgacgaaga tgccgccaag tgcaggggtc gagggcgttg
     3541 tgtgttccga ggtcacttgt ctcctacatt cacaacctca acctgcgtgt gctcgccggg
     3601 ctgaagcgaa acgttgcgga actcaacccc tgtgaacggg cgcgcctcga aacggaactt
     3661 cttcacccgg ctaagcggca gccccgggaa ccgcccttcc gcggatgcca cgtcatctat
     3721 ggaattctca taaggaaacg acacgtgaag ctgccccttg gtgtccaagg ccgtcacgcg
     3781 cgttgtccat cccggcacct tgttgtaaga tacccctacg cgtgcttccc cgctcgattc
     3841 caccggatcg aggaacagtg ccctgcacgt ctggtccccg agtgtgaaac tgctgcccgc
     3901 gccggcctgc ggtgtccgag cgagcattgt tttccactcg cccgctgcga cgccaagcct
     3961 gatcgtaact tctttagccg gctccggcag caccgcctca atcacgttgt ggtcgggcag
     4021 ctccttgccc tggcgccagg ggcggctccc cattgagact tccccggttc ccggttcaaa
     4081 actgcactcc agcgttgtat ccggcgggag gccgcgccgc tggaacacaa attcaaagcg
     4141 ctgccctttg gtgggggagc tgctggtgct tgggttgtgg aagaccacgt tcgtccaagg
     4201 ggttccgtcg ggccgccacc acgtctggtt ggtggaagga tggtgggaca gagccagcag
     4261 ttcaacctcg ccctgcgcca gccgcgcaac gaaaggtagg ccgtgggaag cggcagaccc
     4321 ggtcgtggcg ggggagagtt cagcggaatt ggttgggccg tgccaccatt ggtaagcgag
     4381 cggggcagtt ccggcggcgg tcaccgagaa cgtcgtcgcc gtcgaggcca ctgaggacat
     4441 tggttccgct gcttgcaaaa ccattcggtt tgcaggctga gcctctggct tgcccgtgcc
     4501 ctctggcagc gagttccttg aggtttgcac cagcttgtaa cgaagcttca cgctgcgcgg
     4561 cttatcggtg tattcggtga cgagcagaat gcccgcattg ccctgacggg ttctgaaaag
     4621 gaatgtgaaa tcgcgagtac cccaagcaag cccctgccac caaccatgct ccggcgcgga
     4681 acggactgcc gtcgcgatgg tctgagatga gagtccgtcc caggattcgg tgttggtggg
     4741 aaacatggcc atgtccaggg acctcagctc aagttgcggc acctcgatcg aaccgtccgg
     4801 accggtcttt ccaacgcaga accgaatcaa tacgtccgcg ccgctgctgc gcgcccaggt
     4861 gagcgcctcg tcggctacgc ggagtttctc cggctccgga gtgttcaggc gtccaccggg
     4921 ttccttcatc aacttgccag tttcaaagct caggaacatg tcccgctcgg gttcgctcaa
     4981 gttgattgta cgttcgatca ccgggccgaa gccggccgtg ttggtgttgc ggtggtttac
     5041 gtgggccagt cgggcagaaa ggttcgccga ctgcggcaga accagcttgt agcgaatctt
     5101 cacaccgcgt gggttctcgg tgaagccggt gatttgcagc aggcccagct tggtcacgcc
     5161 ggttttgaaa aagagggtga gtggcagttt acccgggaaa tctccagttt g
//
LOCUS       DATCGS010000457         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_138418, whole genome shotgun sequence.
ACCESSION   DATCGS010000457 DATCGS010000000
VERSION     DATCGS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138418"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VIL39_09405"
     CDS             complement(<1..590)
                     /locus_tag="VIL39_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY5481682.1"
                     /translation="MTQSDRDRLVTLRKARKKLIKQREAAEELGVSVRQVKRLLYALK
                     KRGDRAVIHGLRGVGSKRRIAEKIQRQAIQILSGEDYQGFGPTLASEYLASKHKIEAS
                     RETVRKWMIEAGLWKAGQCSVDAVHVWRPRRSRFGELVQWDTSEHDWLEGRGEKLYLI
                     LLIDDATSRAIGRFARSDSTEANLGVLELWLLRYGR"
     gene            complement(858..2396)
                     /locus_tag="VIL39_09410"
     CDS             complement(858..2396)
                     /locus_tag="VIL39_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEY5481683.1"
                     /translation="MSTRILLALAAVALSALAQQASSASLTIHADQPGPQVNRQLFGQ
                     FAEHLGAGIYGGIWVGEDSKIPNTRGYRNDVLQALRDLKVPVVRWPGGCFADEYNWRE
                     GVGPRSKRPVKINTHWGGVTEPNTFGTHEFMDFAEMIGAEAYISGNVGSASPRELAEW
                     VEYVTSPAGSLASERAANGRQAPWKLPYIGLGNELWGCGGNMRAEYAADVTRRYSTFI
                     KVPAGVRILKSASGPSDEDYHWMEVMMRDAGGRFDSIGLHYYTITHNWQRKGSATAFD
                     EAEYAKTLATTIRIDDIITKHSAIMDKYDPRKRVWLALDEWGTWWDVEPGTNPGFLFQ
                     QNTLRDALVAALNINIFVKHADRLKLANIAQMINVLQAMILTKDEKMVLTPTYHVFEM
                     FKSYQDSTALPVEIQSAWYNKDASTLPEVSASAVRDKDGHLHVALVNVNPSRPVTITA
                     QIPGGAATGVTGRVLTAAAINAHNTFDQPAVVKPAAFTGAAIESGSLKATLPPKSVVV
                     LDLK"
     gene            2786..>3285
                     /locus_tag="VIL39_09415"
     CDS             2786..>3285
                     /locus_tag="VIL39_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481684.1"
                     /translation="MRTMYCSFALGLLISFSALAQPSMLQDPLLDHMIGKWVLQGTIA
                     GKETTHDIVSEWVLGHQYMRIQEVSREKDARNQPAYEAIIFIGWDPQSSQYTCLWLDN
                     TGAWDFTSQAIGRAKRSGDAIAFRFASNDGSSVNTTFSYNKNADAWQWSIDNQTGGKI
                     QDFARV"
BASE COUNT          593 a         1042 c          955 g          695 t
ORIGIN      
        1 atgcgcccat agcgcagcaa ccacaactcc agcaccccca ggttcgcttc cgtgctatcg
       61 ctgcgcgcaa accgtccaat cgcccgactg gtcgcatcgt cgatcaacag aatcagatac
      121 agcttctccc cccgtccttc cagccagtca tgttcgctgg tgtcccattg caccaattct
      181 ccaaaccggc tccgccgcgg ccgccacaca tgcacagcat ccacgctgca ctgacctgcc
      241 ttccacagcc ccgcctcgat catccacttc cgcaccgttt cccggctcgc ttcgatcttg
      301 tgtttgctgg ccagatattc gctggctaag gtcgggccga atccctggta gtcctctccc
      361 gacagaatct ggatcgcttg gcgctggatc ttctcggcaa tccgccgctt cgatcccact
      421 ccgcgcagtc cgtggatcac cgctcggtca ccacgtttct tcaaggcgta cagcagccgc
      481 ttcacctgcc gtacgctcac ccccagttcc tcggccgcct cccgctgctt gatcagcttc
      541 ttccgggctt tccgcagcgt cactaagcgg tctctatcgg attgcgtcat cagtagttgt
      601 ccttcttgca tcccccaagc ttggggttgt caagaggaca tttctacttt gctcaaaggg
      661 gacattatca ctttgcggtg acatccggga ttgcgaaagc gcatacatcg ggcaaatcga
      721 gcctcttacg tgagctggat gcgacgctca gccagttttt ctggggggcg ctttcagagg
      781 gtgtgacccc gagcgggttg tggtcgtgca cagggccgtt ctgccggcca tcttgctttg
      841 tgaacttcgc cttcccgcta tttcaggtcg agcaccacca ctgacttcgg cggcagcgtc
      901 gccttcaagc tgccggactc gatcgccgcg ccggtgaagg ccgccggctt caccaccgcc
      961 ggttggtcga acgtgttgtg ggcgttgatt gccgccgcgg tcagcacgcg ccccgtaacg
     1021 cccgttgcgg caccgccggg aatctgcgcc gtgatcgtta ccggccggct gggattcacg
     1081 ttcaccagcg cgacgtgcag atggccgtcc ttgtcgcgca ccgccgaggc gctcacctcc
     1141 ggcaacgtcg aggcgtcttt gttgtaccag gcggactgaa tctccaccgg cagggcggtg
     1201 ctgtcctggt acgacttgaa catttcgaat acgtgatagg tcggcgtcaa gaccatcttc
     1261 tcgtccttgg tgaggatcat ggcctgcagc acgttgatca tctgcgcgat gttggctagc
     1321 ttcagccggt cggcatgctt gacgaagatg ttgatgttga gcgccgccac cagcgcgtcg
     1381 cgcagcgtgt tctgctggaa caggaagccg ggattggtgc ctggctcaac gtcccaccag
     1441 gtgccccact cgtccagggc cagccagacg cgcttgcggg gatcgtactt gtccatgatg
     1501 gcggaatgct tggtaatgat gtcgtcgatg cggatggtgg tcgccagcgt cttggcgtat
     1561 tcggcctcgt cgaacgcggt cgccgagcct ttccgctgcc agttgtgcgt gatggtgtag
     1621 tagtgcaggc cgatggagtc gaagcgcccg ccggcgtccc gcatcatgac ttccatccag
     1681 tgataatcct cgtcggatgg accggaggcc gatttcagga tcctcactcc cgccggaacc
     1741 ttgatgaatg tggagtaccg acgcgtcacg tcggcggcat actccgcccg catgttgccg
     1801 ccgcagcccc agagttcgtt gccgagcccg atgtacggca gtttccaggg agcctgacgc
     1861 ccgttggctg cccgctccga agccagcgat ccagccggcg acgtcacgta ctccacccac
     1921 tccgccaact cgcggggtga cgccgaccct acgttaccgc tgatgtaggc ctcggccccg
     1981 atcatttccg cgaagtccat gaactcgtgc gtgccgaacg tgttcggctc cgtgaccccg
     2041 ccccaatgcg tgttgatctt gaccggccgc ttggatcgcg gtcctacgcc ttcccgccag
     2101 ttgtactcgt cggcgaagca gccgccgggc cagcggacca ccgggacctt caggtctcgg
     2161 agagcctgca gaacgtcgtt gcggtatccc cgcgtgttcg gaatcttgga gtcttcaccg
     2221 acccagatgc cgccgtagat tccggcaccc aggtgctcgg cgaactggcc gaacagttgc
     2281 cgattaacct gcggtccggg ctggtcggcg tggatggtga ggctggcgga cgaagcctgc
     2341 tgggccaggg cggacagggc cacggcggcg agagcgagga gaattcgagt agacatgata
     2401 gcgctagcat tttacagcat ttcacggcat gagccatccc cttacctaat cggtgtagcg
     2461 gatttccggc agacaacctc tcagggggcc gcatcgatct cgccctttaa ggtccgcagt
     2521 tacacgcttg ctccgggtgt atggtcggcc atccggacac gatccccggt cggctgattc
     2581 tgtccgggtt gcgcgtctta cggacggaat tggtaaacgc ggggcgactc tgcaatgcct
     2641 atccaagaat catccgggcc caggcccgag aggcagttta gtgccgagat cagaagacgc
     2701 gaattcgtgg cggtgtgaag cagtgaagag actctcttgc catcgatcgg atgcccagga
     2761 gatcattaca actggaggcg tccaattgcg cacaatgtat tgctcctttg ccttaggtct
     2821 actgatttca ttctccgcgc tggcccaacc atcaatgctt caggatccgt tactcgatca
     2881 catgatcgga aagtgggtgc ttcagggcac catcgccggg aaggaaacga cgcacgatat
     2941 cgtgtcggag tgggtactgg gtcatcaata catgcgaatc caggaagtgt cccgagagaa
     3001 ggacgccagg aaccagccgg cgtatgaagc gattatcttc atcggctggg atccgcagtc
     3061 gagccagtac acctgtctgt ggctcgacaa tactggcgct tgggacttca catcccaggc
     3121 aatcggtcgc gcgaagcgaa gcggcgacgc catcgcattc cggttcgcaa gcaatgacgg
     3181 cagcagcgtc aacacaactt tctcctacaa caagaatgcc gacgcttggc agtggtcgat
     3241 cgacaaccag acgggcggca agattcagga cttcgcacgg gtaag
//
LOCUS       DATCGS010000458         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_485184, whole genome shotgun sequence.
ACCESSION   DATCGS010000458 DATCGS010000000
VERSION     DATCGS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485184"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VIL39_09420"
     CDS             complement(<1..266)
                     /locus_tag="VIL39_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY5481685.1"
                     /translation="MFSTSSIIVGLEIGTSKVCAVVGELAADGALNIVGLGQSRSRGV
                     RKGEIADAPLAEEDVRHAIVEAEQMADVEIRSVYLGVTGSHLRG"
     gene            complement(259..1218)
                     /locus_tag="VIL39_09425"
     CDS             complement(259..1218)
                     /locus_tag="VIL39_09425"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY5481686.1"
                     /translation="MWFKRKPKNRRLGREQVLDVKLRSSQVRAARTRMGAISVGAVFA
                     TVFGLYLLYRTGEWVLNRCVYENKAFAIQEIDVQTDGVISVDQLRRWAGVRPEENLLA
                     LDLARVKRDLEMVPLVQAVSVERILPHTLRLRITEREPVAQVNVHRPRRSGGVESAVY
                     QLDADGYVMLPLDPRQRATPVNQPADSLPVVGGINASELQPGRRVLAPSVQAALQLVM
                     AFDRSPMAGAVDLKRVDASSPAVLVVTTGQGSEVVFGLADLEQQLRRWYDIFNLGQKM
                     SKAIATLDLAVTNNIPARWIEASAVPPAAPKSPKALRPKKRHV"
     gene            complement(1208..2131)
                     /locus_tag="VIL39_09430"
     CDS             complement(1208..2131)
                     /locus_tag="VIL39_09430"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY5481687.1"
                     /translation="MNLADKKLNITVMLGGPSAEREVSLRSGAEVAKALRSLGHQVHE
                     VDPRNEKWKLPRVTDVVFLALHGTYGEDGTVQRRLEELGATYTGCDPEASRIGFDKFL
                     TKERCVAAGVPTARFLLIESPLASWPMGWDPPVVLKPARQGSSVGLQFVERVSDWSKA
                     LAEAMRHDSQVLMEEKISGRECTVGILADEPLPLVEVRPKTGIYDYQTKYSVGATDYF
                     CPAPFDEATTAHAQAAGLAAFKAIGGRDYSRTDVIVRPSGEPVVLEVNTLPGMTETSL
                     LPKAAAAAGISYPELCQRMVDLALKRRKNVV"
     gene            complement(2128..2946)
                     /gene="murB"
                     /locus_tag="VIL39_09435"
     CDS             complement(2128..2946)
                     /gene="murB"
                     /locus_tag="VIL39_09435"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY5481688.1"
                     /translation="MRVGGPADVYVEPGSEQDLAAILAWCHEGGRRFFLLGRGSNLLV
                     KDDGFRGVVICLAHAQFSRIEVAGARLNCGAGVKLKAVAVAASRNDLAGLEFLEGIPG
                     TVGGALRMNAGAMGRAMLDVVESVRLMSFDGSVHERLAPELAVAYRSCPALKTHIALA
                     AVLRGQPGSREIIEQVMNEYSRKRWQSQPAAPSAGCIFKNPPSIPAGKLIDELGLKGT
                     RVGGAVVSAEHGNFIVNSGTATARDMFDLIEIIKQRVRAERGIELETEVEIVGE"
BASE COUNT          592 a         1008 c          884 g          572 t
ORIGIN      
        1 aagccgcgca agtggctgcc cgtcaccccg agatagacac tgcggatctc gacatcggcc
       61 atttgctccg cttcaacaat agcgtggcgc acgtcttctt cggccagcgg cgcatcggcg
      121 atttctccct tgcgcacgcc ccgcgagcgg gactggccga gaccgacgat gtttagcgcg
      181 ccgtcggcgg cgagttcgcc aaccacagcg cagactttgg aggtgccgat ttccagtcct
      241 acgataatcg aagaggtgct aaacatgtcg tttctttggg cgtaaggctt tgggtgactt
      301 cggagccgcg ggaggaaccg cgctagcctc aatccagcgc gccggaatat tattggtaac
      361 cgcgagatcg agagtggcaa tggccttgct catcttctgg cccaagttga agatgtcata
      421 ccatcggcgc agttgctgct ccagatctgc caggccaaaa accacctcgc tcccctgccc
      481 cgtcgtcacg accagaaccg ccggggacga cgcgtcaacg cgtttcagat ccactgcccc
      541 ggccatcggc gaccggtcga aggccatgac cagttgcagc gcggcctgga ctgaaggggc
      601 aaggacgcgc cggccgggct gcagttcgct ggcgttgatg ccgccaacca cagggagcga
      661 atcagccggc tggttgaccg gcgtggcacg ctggcgcggg tcgagcggca acatgacata
      721 accgtccgcg tccaactgat atacggccga ctcaactccc ccgcttcgac gcggccgatg
      781 gacgttgact tgtgctaccg gttcgcgctc ggtaatacga aggcgcaggg tgtgcgggag
      841 gatacgctca accgaaacag cctgaaccaa agggaccatt tccaggtcac gcttgacgcg
      901 cgcaagatca agcgcgagca gattttcctc gggccgcact cccgcccagc ggcgcagttg
      961 gtccaccgag atgacgccgt cggtctgcac gtcgatctcc tggatggcga atgctttgtt
     1021 ctcatagaca caccggttga ggacccattc tccggtgcga tagagcagat acaagccaaa
     1081 gacggtcgcg aagacggcgc ccacgctaat cgcgcccatg cgcgtgcgcg cggcacggac
     1141 ctggctggag cgcaatttca cgtccagcac ctgctcgcga ccgagtcgcc gattctttgg
     1201 cttgcgttta aaccacattt tttcttcgct tcagcgccag gtccaccatc cgctggcaca
     1261 gctccgggta gctgattccc gcggcggcgg ccgctttggg cagcagactg gtttccgtca
     1321 tgcccggcag cgtgttcact tccagcacca ccggttcgcc gctggggcgc acgatgacat
     1381 ccgtccgcga gtaatcacgg cctccaatcg ccttgaaggc cgccagaccc gcagcctgag
     1441 catgcgcagt cgtcgcctcg tcgaacggcg cggggcaaaa atagtcggtc gccccgacgc
     1501 tatacttggt ctggtagtcg taaatgcctg tcttgggccg gacctcgacc aggggcagcg
     1561 gttcgtcagc aagaatgccg acggtgcatt cgcggccgga aatcttctct tccatcagaa
     1621 cctgtgaatc gtggcgcatc gcctcagcaa gcgccttgct ccaatctgag acgcgctcga
     1681 cgaattgcag gccgacactg gaaccctggc gggcgggttt gagcacgacg ggggggtccc
     1741 agcccatcgg ccagcttgcc aagggcgact cgattaacag gaagcgggcg gttggaacac
     1801 ccgccgccac acaacgctcc ttggtgagga acttgtcaaa cccgatccgg ctcgcctcgg
     1861 ggtcgcatcc cgtgtaggtc gcacccagtt cctccaggcg acgctggacc gtgccgtcct
     1921 cgccgtaagt tccgtgcagc gcgaggaaaa cgacgtcggt gactctgggc agcttccact
     1981 tctcgtttct cggatccacc tcatggactt ggtgccccag ggagcggagc gccttggcca
     2041 cttccgcgcc ggagcgcagg gagacttcac gttcggcgct cggtccgccg agcatcacag
     2101 tgatgttgag tttcttatca gcaaggttca ttcgccaacg atttcgactt ccgtttccaa
     2161 ctcgattccg cgttctgccc ggacacgttg cttaatgatc tcgatcaagt cgaacatgtc
     2221 ccgcgcggtg gccgtgccgc tattaacaat aaagttgccg tgctcggccg aaaccaccgc
     2281 gcccccaacg cgcgttcctt tcaagcccag ttcgtcaatc agcttgccgg cggggatcga
     2341 gggcgggttc ttgaatatgc acccggcgct cggcgcggcg ggttgcgatt gccaccgctt
     2401 ccggctgtac tcgttcatga cctgctcgat gatctccctc gagccgggct ggccgcgcaa
     2461 caccgccgcc aaggcaatgt gcgtcttcaa agctggacaa ctgcggtagg cgaccgccag
     2521 ttccggggcc agtcgctcat gcacgctccc gtcgaaactc atcaagcgaa cggactcaac
     2581 aacgtcaagc atggcgcggc ccattgcccc ggcgttcatg cgcaaagcgc caccaacagt
     2641 gccggggata ccctccagga attccagtcc agccagatcg tttcgactgg ccgcgaccgc
     2701 gaccgccttc agtttcacgc ccgcgccgca gttcagccgc gcacccgcca cctcgattcg
     2761 gctgaactgg gcgtgtgcca gacaaatgac cacgccacga aagccatcgt ccttcaccaa
     2821 cagattcgag ccgcgcccga gaaggaagaa acgccggcct ccctcatgac accaagccag
     2881 gattgccgcc aaatcctgtt ccgaacccgg ttcaacataa acatccgccg gcccgcccac
     2941 ccgcagggtc gtgtgtcaac ccctctatgg gagtagcaat ctggtacaaa gcaatcatac
     3001 gaaggcgact cttggtattg gcatatgcta aggcgtcggc atagtcatac ttagta
//
LOCUS       DATCGS010000459         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_233977, whole genome shotgun sequence.
ACCESSION   DATCGS010000459 DATCGS010000000
VERSION     DATCGS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233977"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIL39_09440"
     CDS             <1..217
                     /locus_tag="VIL39_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481689.1"
                     /translation="PEATAAYMAQQAAATTNGPCFLWARTILKTPTWHAKVSGILREK
                     HPNAPVVVVDPYTFFGLIKEQANGERK"
     gene            306..1184
                     /gene="rfbA"
                     /locus_tag="VIL39_09445"
     CDS             306..1184
                     /gene="rfbA"
                     /locus_tag="VIL39_09445"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY5481690.1"
                     /translation="MKGIILAGGAGSRLFPLTLVASKQLQPVYDKPMVYYPLTTLIEG
                     GIRELCLISTPQDLPRFRQLLGDGSAWGLSIDYREQAKPEGIAQAFLIAESFIGKHAV
                     TLILGDNVFYGGDSFYKAFADFKGGATIFGYHVNDPERYGVVEFDGNGQAISIQEKPR
                     QPRSNYAVPGLYIYDNSVVTITRNLKPSARGELEITDVNVEYMRRKQLHVHRLSRGFA
                     WLDAGTSSSLHEASAYVQTIEKRVGVKIGCPEEAAFRRGFLSLEGLDALTAKIPKCEY
                     REYLAEVVAEAKRLGH"
     gene            1191..1739
                     /gene="rfbC"
                     /locus_tag="VIL39_09450"
     CDS             1191..1739
                     /gene="rfbC"
                     /locus_tag="VIL39_09450"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY5481691.1"
                     /translation="MNVLTCDIPDLLIIEPKVFGDARGFFLETWNLHRYREAGIDADF
                     VQDNVSFSRRGILRGLHCQNPKPQGKLLEVLQGEVFDVAVDVRRSSPTFGKWHGLVLS
                     SENKRQFYIPPGFAHGFAVLSETALFHYKCTEFYSPKDEMAIRWDDPDIGIKWPLKEP
                     LLSERDAKALLLRDVPPERLFD"
     gene            1750..>2180
                     /locus_tag="VIL39_09455"
     CDS             1750..>2180
                     /locus_tag="VIL39_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5481692.1"
                     /translation="MQPPRILLIGKIGQVGWELRRTLAPMAQVACVDYPEIDLTTGDS
                     IRRWVRETQPNIVINAAAYTAVDKAESEPDKAMKINGVAPGILAEEAKKLGALLVHYS
                     TDYVFDGAKTEPYVETDAPNPLGAYGRTKLAGDEAVRAIGGA"
BASE COUNT          453 a          638 c          666 g          423 t
ORIGIN      
        1 gcctgaagca accgccgcct atatggcaca gcaggcggcg gccacaacca acggcccgtg
       61 ttttctgtgg gccaggacga tattgaagac cccgacctgg cacgcgaagg tgtcgggaat
      121 cctgcgcgag aagcacccca atgcgccggt ggtggtagtt gatccgtaca cgttcttcgg
      181 cttgatcaaa gaacaagcaa acggagagcg gaagtaaggc ccaactcagc gctttccgaa
      241 cgcctcttgc gtcgggtatg tttgcgcgtt gcaattcggc actcatgaat tatcctcacc
      301 ggctgatgaa aggcataatc ctggctggtg gcgccggttc aaggctgttc ccgcttaccc
      361 tggtagcgag caagcagctt cagccggtct atgacaaacc gatggtgtat tacccgttga
      421 cgacgctgat tgaaggcggc atccgggagc tatgcctcat ctccacaccg caggatttgc
      481 ctcgtttccg ccagttgctc ggggacggca gcgcctgggg gttgtccatt gattaccgcg
      541 agcaggccaa gccggaaggc atcgcccagg cgtttcttat tgccgagagt tttattggca
      601 agcatgccgt cactctaatc cttggggata acgtcttcta cgggggcgac agtttctaca
      661 aagccttcgc tgatttcaag gggggcgcaa cgatcttcgg gtatcacgtc aacgacccgg
      721 aacggtatgg cgtggtggag tttgacggca acggccaggc gatttccatc caggagaagc
      781 ccaggcaacc caggagcaac tacgccgttc ccgggctcta catttacgac aacagcgtgg
      841 tgacgattac ccggaatctg aagccgtccg cgcgggggga gctggaaatc accgatgtga
      901 atgtcgaata tatgcggcgc aagcaactgc acgtgcacag gctcagccgc gggttcgcct
      961 ggctggatgc cggcacgagc agcagtttgc acgaggcttc agcctacgtg cagaccatcg
     1021 agaagcgcgt cggcgtaaag attggctgtc cggaggaggc ggcttttcgc cgtggtttcc
     1081 tgtcgctgga agggctggat gccctcacgg cgaagatacc caagtgcgag tatcgcgagt
     1141 atctggccga agtggtcgcc gaggcaaagc ggttgggcca ctgacacccc atgaacgtcc
     1201 tgacctgtga tatccccgac ttgctcatta tcgagcccaa ggtgttcggc gacgcgcgcg
     1261 gcttcttcct cgaaacgtgg aacctgcacc gctaccgcga ggctggaatt gacgcggact
     1321 tcgtgcaaga caacgtttca ttctcgcgcc gcggcatttt gcgcgggctg cattgtcaga
     1381 acccgaaacc gcaaggcaaa ctgctggagg tgctgcaagg ggaagtgttt gacgtcgcgg
     1441 tggatgtccg gcgcagctca cccaccttcg ggaagtggca cggcctcgtg ctctcaagcg
     1501 agaacaagcg gcagttctac atccctcctg gattcgcgca cgggtttgcc gtgctcagtg
     1561 aaaccgcgct cttccactac aagtgcaccg agttctactc gccgaaggac gaaatggcca
     1621 tccgctggga cgatccggac atcgggatca aatggccgct gaaagaacct ctgctctccg
     1681 agcgtgacgc caaggcgctg cttctccgcg acgtgccgcc cgaacgctta tttgactgaa
     1741 gcgcgatcca tgcaaccgcc tcgcatcctg ctcataggga agatcggcca ggtcggctgg
     1801 gaactgcgcc gcacgcttgc cccaatggcc caagtcgcct gcgtagatta tcccgagatt
     1861 gacctgacca ccggcgactc tattcggcgg tgggtgcgtg aaactcagcc caacatcgtt
     1921 atcaatgctg cggcttacac cgcggtggac aaggccgagt ccgagccgga caaggcaatg
     1981 aagatcaacg gcgtggcccc gggcattctg gctgaagaag ctaagaaact gggcgcgctg
     2041 ctggtgcatt actcgacgga ttacgtgttc gacggggcca agaccgagcc atacgtcgag
     2101 accgatgcgc cgaatccgct gggcgcttac ggacgcacta aactggccgg tgacgaagct
     2161 gtgcgggcca tcggcggcgc
//
LOCUS       DATCGS010000460         9835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_732146, whole genome shotgun sequence.
ACCESSION   DATCGS010000460 DATCGS010000000
VERSION     DATCGS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732146"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="rpoB"
                     /locus_tag="VIL39_09460"
     CDS             <1..998
                     /gene="rpoB"
                     /locus_tag="VIL39_09460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414634.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5481693.1"
                     /translation="TGEIIIPANRKITKTLLRKLAQVYDRIEIDPSPIRNKINEIIGS
                     FKKKFDDLQMQYDEELERAESGEGVDSGVVKSVKVYIASKRKLSVGDKMAGRHGNKGV
                     VAKIVAEEDMPFLADGTPVDIVLNPLGVPSRMNVGQVLETHLGWAARLLGLKFATPVF
                     DGIKEKDIRGYLKEAKLPEHGKIKLIDGRTGEPFDQEIVVGFIYMMKLGHLVADKIHA
                     RAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWAMEAYGAAYTLQELLTVKSDDVQGRTR
                     IYESIVKGDNSLEAGIPESFNVLVKEMQSLGLDVKVGYSNPVDQPAEPIQALTAV"
     gene            1048..5193
                     /gene="rpoC"
                     /locus_tag="VIL39_09465"
     CDS             1048..5193
                     /gene="rpoC"
                     /locus_tag="VIL39_09465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY5481694.1"
                     /translation="MISSKESARELLGLEKVNQVEYVAIGVASPDSIRSWSKGEVKNP
                     ETINYRTFKPEKGGLFCERIFGPVKDWECSCGKFKRIKHRGVICDRCGVEVTLARVRR
                     ERMGHIELAVPVCHIWFFKCMPSRIGLVLDMTARNLERVIYYEDYMVIDPGSTPLKQN
                     QLLSEHEYREARETYGPDAFVAKMGAEAVREGLSKVDLGKQIDYLQTAMTETKSKQIR
                     KKIAKRIKLLQGLQSSKSRPEWMILMVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRV
                     INRNNRLKNLLQLKTPEVIIRNEKRMLQEAVDALFDNGRHGRAVTGAGNRPLKSLSDM
                     LKGKSGRFRQNLLGKRVDYSGRSVIVIGPELKLHQCGLPKKMALVLFEPFIIRRLKEL
                     GYVHTVRSAKKLIERQTTEVWDILEEVTRGHPVMLNRAPTLHRLSIQAFEPQLIEGEA
                     IRIHPLVCTAYNADFDGDQMAVHVPLSVEAQLEARLLMMAPNNIFSPSSGRPIITPTQ
                     DITLGCFYLTADPRTPPPADSKLLKLFGSKSEVVFAFMDGAVTTHERIRLANPDCGKK
                     TELGDPNKKVIETTVGRVFFSEIWPDELGFPNKPVKKSELGDIIWKCYKICGHDKTVV
                     MLDKLKELGFREATRAGVSIGIDDMIIPKEKGQEIDTAQKQIREVEKQYRKGVITPGE
                     RYNKIIDIWTHCTDQIANVMLKTLDHNQGKKEYNPVSLMVDSGARGNRQQVRQLAGVR
                     GLMAKPSGDIIEKPILSNFREGLTVLEYFISTHGARKGLADTALKTADSGYMTRKLVD
                     VAQDVIIREEDCGTTNGIWVQAIYEGEDKVVKLSDRLVGRFSCDDIPNPQKPKELLAK
                     ANEEIDEVKARLIDSADIEKVKIRSVLTCESKSGICICCYGRNLATGAKVKLGEATGI
                     IAAQSIGEPGTQLTMRTFHIGGTASAVFKVPQIKAKHDGTIRYNDLRLVTLEDGKNNI
                     VLNKNGSVSVLADDGRELEQHTVVIGAVISFADGAKVKKGDIFVQWDPYNVPILSEKA
                     GKVKFQDIIEGVTMKQETDETTSQEAMVVIEHKEDLHPQIIIFDDKGEPEANYPIPSG
                     AHIVVAEGDKIVAGTLMAKTPRKTSKTKDITGGLPRVAELFEARRPKDAAEISKIEGI
                     VDFGASIRGKRCIVIKDQQTGVEEEHLIPIGKHVIVFKGDYVKKGQQLTEGPIDPHEI
                     LDICGPQELQEHLVNEVQEVYRLQGVTINDKHVETIVRQMLRKVRITEPGDTGFLWGE
                     QIEKLDFEDENQRVEKMGGKPAEAQPVLLGITKASLETESFLSAASFQDTTRVLTEAA
                     TRAKVDYLRGFKENVIMGHIIPAGTGFDYHRKVRLKPLVEVSEDEPEAPKDLTASENP
                     LLA"
     gene            complement(5373..7250)
                     /locus_tag="VIL39_09470"
     CDS             complement(5373..7250)
                     /locus_tag="VIL39_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481695.1"
                     /translation="MSVSPTSKSPIAPAADKLGTAREAITYFRPIEEREADHAPLSAE
                     LIRRMFTYTRRYTARRNWLFVLTFARGLQLPALAWMIGQTINGPIAGRNLPAIYLHAG
                     IYLVLVLLMVVTLHFRQRFALELGEAVAHDMRLELFRKLATLPMSFFNKTKFGRIISR
                     MTSDIDSVRVAVQDVAFVVTIQAVQMTVAALLMAWYNWKLFSLMLLLVPGIWVVNLNY
                     RRKVSHQLRKLQETWSRLTSTLAESVSGIRVTQAFVRQEINAGFFRKLVNLHGENNVG
                     VARASAVFIPLLQMKSQLFLGAVALLSGYGALRWHGWLHMEVGDLVMFFFLANFFFDP
                     VQVIGNQYNQALSAMAGAERYFRLIDLEPEWQDALTAKALPPIQGRVEFQEVHFEYER
                     GRPVLADITFVAEPGQTVALVGHTGSGKTTLVGLLQKLYLPTRGRVLVDGHDLIEVTS
                     DSLHSQMGSVQQNNFLFAGSISDNIRFARPETSEADVRATLRALNCLDLVEALPRGLE
                     TQVGEKSAALSLGQRQLICFARALLADPRIVVLDEATSAIDSVTEARLQRALEILLRG
                     RTAFVVAHRLSTIRKAGLVLVMDQGRIVERGTHTTLLAAGGVYSRLHEEFIRGGSQAG
                     G"
     gene            complement(7247..9115)
                     /locus_tag="VIL39_09475"
     CDS             complement(7247..9115)
                     /locus_tag="VIL39_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481696.1"
                     /translation="MSDSPPNKHYERRYATGELVRRLLGLAWQFRGDCLRSLVLSVML
                     LLLGLLGLQLLGVVIDVIRHALDPTQRAPVYPFGWGPPAGWTPLTIVTVMALAIVAQA
                     VLRAFLTYHYNMTTALLTQGKIVPDLRDRVYDKLQRLSFRFFDVHGSSSIFNRVTGDV
                     QNTRLFVDGVVLQGVNMLLTLAAYFVFMWRIQPSLTLACLSVTGGLWWVTHYYSGRLR
                     PAYLRNRELYDSLVLTFTESIRGMQTVKGFAAEPHQIRRFEEANARVSDQQKRIFRDL
                     SNFTPLTQVLSQVSLVILFAYGGWLYVQGRIPLGSGLVVFAGLLQQFNGQVANITTIA
                     NSVQQSFTAARRVFEVLDTPSEVQNRPNAIVPGRLTGRLAFEGVTFGYVPEASVLQDV
                     SFEAAPGQVIGIFGMTGAGKSSLLSLIPRFYDPQHGRILADGRDLRDLDLDAYRRQIG
                     IVYQESFLFSNTVAANIAFGNPHATQEQIERAARTASAHEFIVAMPHGYETVLGESGV
                     DLSGGQRQRLALARALLLEPPILILDDPTASVDPKTEHEIVSALRQAMAGRTTFVVAN
                     RLSLLRRADVILVLEKGRLLHVGTHDELAHVPGTYRETALLQLMDLTEEQGRDQGP"
     gene            complement(9633..>9835)
                     /locus_tag="VIL39_09480"
     CDS             complement(9633..>9835)
                     /locus_tag="VIL39_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEY5481697.1"
                     /translation="LLPALSRARDAARRTTCISNARQINLALRMYADDHQDAIRAVTN
                     KEALYGSLPASCSWASFPVPAG"
BASE COUNT         2084 a         3021 c         2974 g         1756 t
ORIGIN      
        1 aaaccgggga aatcatcatt cccgcgaatc gcaagatcac caagacactc ctgcgcaagc
       61 tggcgcaggt ttacgaccgc atcgaaatcg acccatcgcc gatccgcaac aagatcaacg
      121 aaatcatcgg cagtttcaag aagaagttcg acgatctgca aatgcagtac gacgaggagc
      181 ttgagcgcgc cgagtccggc gaaggcgtcg atagcggtgt cgtcaagtct gtcaaagttt
      241 atatcgcctc gaagcgcaaa ctgtcggtgg gtgacaaaat ggccggtcgc cacggtaaca
      301 agggtgtggt cgccaagatt gtggcggagg aggacatgcc cttcctggcg gatggaacgc
      361 cggtggacat cgtgctgaac ccgctgggtg tgccctcgcg catgaacgtc ggccaggtat
      421 tggaaacgca cttgggttgg gcggcacgac tgctgggcct gaagttcgct acgccggtat
      481 tcgacggcat caaggaaaag gacattcgtg gttacctgaa ggaagctaaa ttgccggagc
      541 acggcaagat caagcttatc gacggccgca cgggcgagcc gtttgatcag gaaatcgtcg
      601 tcgggttcat ttacatgatg aagctaggcc acttggtggc cgataagatt catgcccggg
      661 ccgtcgggcc ctattccctg gtcacccagc aaccactggg cggcaaggcg caatatggtg
      721 gccagcgctt cggcgaaatg gaggtttggg ctatggaagc ctacggggcc gcctacacgc
      781 tccaggagtt gttgacggtc aaatccgacg acgtccaggg gcgcactcgg atatacgaga
      841 gcatcgtgaa aggcgacaac agtctggagg ccggcattcc ggagtcgttc aacgtgctcg
      901 tgaaggaaat gcagtcgctg ggacttgatg tgaaggtggg ttactccaat ccggtggacc
      961 agcctgctga acccatccag gccttaacgg ccgtttaact gagataggaa ttctagtgcg
     1021 agacagttcg ccccaacgat tcattgtatg attagctcta aagaaagcgc ccgagagttg
     1081 ctcggtttgg aaaaggtcaa ccaggtggag tacgtcgcga ttggtgttgc gtcgcccgat
     1141 agcatccggt cctggtccaa aggggaagtc aaaaaccccg agaccatcaa ttaccgcaca
     1201 ttcaagccgg aaaagggcgg tctattttgt gagcgcattt tcggcccggt caaggattgg
     1261 gaatgctcgt gcggcaagtt caagcgcatc aagcatcgcg gggtcatctg cgaccgttgc
     1321 ggggtggaag tgacgctggc ccgcgttcgc cgcgagcgca tggggcacat cgagctggcc
     1381 gtgccggttt gccatatctg gttcttcaaa tgcatgcctt cccgcatcgg cctggtgctt
     1441 gacatgaccg cccgcaacct cgagcgggtg atttactacg aagactacat ggtcatcgat
     1501 cccggatcga ctccgctcaa acagaatcag ctcttgagcg aacatgagta tcgcgaagcc
     1561 cgcgaaacct acggccccga tgcgttcgtt gccaagatgg gcgctgaagc ggtgcgcgaa
     1621 ggcctgtcga aagtggacct cggcaagcaa attgactact tgcagacggc catgaccgaa
     1681 accaagagca agcaaatccg caagaagatc gccaagcgca tcaagctgct ccagggtctg
     1741 cagtcttcca agagccgtcc ggaatggatg atcctcatgg tgctgccggt catcccgccg
     1801 gacctccgcc cgctagtgcc gctcgaaggc ggccgctttg ccacctccga tctcaacgac
     1861 ctttatcgcc gcgttatcaa ccgcaacaac cggttgaaga acctgcttca gctcaagacg
     1921 ccggaagtca tcatccgtaa tgaaaagcgc atgctgcagg aagcggttga cgcactgttc
     1981 gacaacggtc ggcacggtcg cgcggtgact ggggccggta atcgcccctt gaagtcactc
     2041 agcgatatgc tcaagggcaa gagcgggcgc ttccgccaga acctcctggg caaacgcgtg
     2101 gattactccg gacgttcggt cattgtcatt ggtccggagc tcaaactgca ccagtgcggt
     2161 ttgcctaaga aaatggcgct cgttctgttc gagcccttca tcatccgtcg tctcaaagaa
     2221 ctgggctatg tgcataccgt gcgatcggct aagaagctga tcgagcgcca gaccaccgag
     2281 gtgtgggata ttcttgaaga agtgactcgc ggtcatcccg tgatgctcaa ccgcgccccc
     2341 actttgcacc ggttgtcgat tcaggcattc gaaccgcagc tcatcgaagg cgaagccatt
     2401 cgcatccatc cgctggtctg taccgcctac aacgccgact tcgacggcga ccaaatggcc
     2461 gtccatgtgc cgttgtccgt cgaagcgcaa ttggaagcgc gcctgctcat gatggcgccc
     2521 aataacattt tcagcccgtc cagcggccgg ccgattatta cgccgacgca agacatcacg
     2581 ctgggatgct tctatctgac tgccgatccg cgcacgccgc cgccggcgga ttcgaagctg
     2641 ttgaagctct tcgggtcaaa gtccgaagtc gtgttcgcct tcatggatgg tgcggtcacg
     2701 acccacgagc gcattcgcct ggcgaatccc gattgcggca agaagaccga gcttggcgat
     2761 cccaacaaga aggtcatcga gaccacggtg ggccgtgttt tcttcagtga aatctggcca
     2821 gatgaactgg gcttccccaa taagccggtc aaaaagagcg agctcggtga catcatctgg
     2881 aagtgttata agatttgcgg tcatgacaag acggtggtca tgctcgacaa gctgaaggaa
     2941 ttgggcttcc gcgaggcgac gcgtgcgggc gtgtccatcg gtatcgacga catgatcatt
     3001 cccaaggaaa agggtcagga aatcgacacc gctcagaagc aaatcagaga agtcgagaag
     3061 cagtatcgca agggcgtcat cacccctggc gagcgctaca acaagatcat cgacatctgg
     3121 acgcactgta cggaccagat tgcgaacgtt atgttgaaga cgctcgacca caaccagggc
     3181 aagaaggagt acaacccggt ttcgctcatg gtggattcgg gcgcgcgcgg caaccggcag
     3241 caggtgcgcc agctcgccgg cgtgcgcggt ctcatggcca aaccgtccgg cgacatcatc
     3301 gagaagccga ttctctccaa cttccgcgag ggcctcacag tgctcgagta cttcatttcg
     3361 actcacggcg cccgcaaggg tctggccgac acggcgctca agaccgccga ctcgggttac
     3421 atgacccgca agctggtaga tgtggcgcag gacgtgatta ttcgcgagga ggattgcggc
     3481 accaccaacg gtatctgggt tcaggcgatc tatgagggcg aagacaaggt ggtcaagctg
     3541 agcgaccgtc tggtggggcg cttttcgtgc gacgacatcc cgaatccgca gaagccgaag
     3601 gagttgctgg ccaaggcgaa cgaggagatt gacgaggtca aggctcggct catcgacagt
     3661 gcggatatcg agaaggtcaa aattcgttcg gtgctgacct gcgaaagcaa gagcggcata
     3721 tgcatctgtt gctatggccg taacctggcc acgggcgcca aggtgaaact gggcgaagcc
     3781 accggtatca tcgccgcgca atccatcggc gagccgggca cgcagctcac tatgcgcacg
     3841 ttccacatcg gcggcaccgc ttccgccgtg ttcaaggtgc cgcagatcaa ggccaagcac
     3901 gacggcacta ttcgctacaa cgatctgcgc ctggtcaccc ttgaagacgg caagaacaac
     3961 atcgtcctca acaagaacgg cagcgtttcc gtccttgccg acgacgggcg cgagctggag
     4021 caacatacgg tcgtcattgg cgcggtcatc tccttcgcgg atggcgccaa ggttaagaag
     4081 ggcgacatct tcgtccagtg ggacccgtac aacgtgccga tcctctccga gaaggccggc
     4141 aaggtgaagt tccaggacat catcgagggc gtcaccatga agcaggaaac ggatgaaacc
     4201 accagccagg aggcgatggt cgttatcgaa cacaaggaag atttgcaccc gcaaatcatc
     4261 atcttcgacg ataagggcga gcccgaggcc aactatccga tcccctccgg cgcgcacatc
     4321 gtcgtggccg agggtgacaa gattgttgcc ggcaccctga tggcgaagac cccgcgcaag
     4381 acctcgaaga cgaaggacat caccggtggt ctgccgcgtg tggccgagct ttttgaggcc
     4441 cgccggccca aagacgccgc ggagatttcc aagatcgagg gcatcgtgga cttcggcgcc
     4501 agcatccgcg gcaagcgctg tattgttatc aaagaccagc agaccggcgt ggaagaggag
     4561 catctcatcc ccatcggcaa acacgtcatc gtcttcaagg gcgattacgt caagaaaggc
     4621 cagcagctca ccgaaggccc aatcgacccg cacgaaatcc tcgacatctg cggcccgcag
     4681 gaattgcagg agcacctcgt caacgaggtt caggaagtct atcggctgca aggcgttacc
     4741 atcaacgaca agcacgtcga gaccatcgtg cgccagatgc tgcgaaaggt gcgcatcacc
     4801 gagccgggcg acaccgggtt cctctggggt gaacagatcg agaagctcga cttcgaagac
     4861 gagaaccagc gcgtcgagaa gatgggcggc aagccagccg aggcccaacc ggtgctgctt
     4921 ggcatcacta aggcctcgct ggagaccgag agtttcctga gcgctgcctc cttccaggac
     4981 accacccgcg tgctgaccga agcagcaacc cgcgccaagg tggattacct gcgcggcttc
     5041 aaggagaacg tcatcatggg tcacatcatc cctgccggca ctgggttcga ctaccaccgc
     5101 aaggtgcggc tcaagccgct ggtggaagtc agcgaagatg agcccgaagc gccgaaagat
     5161 ctgaccgcca gcgagaatcc gctgctggcc tgacccgcca tcacaccgct tacacgaaga
     5221 gcatccgggg aaccggatgc tctttttgct tcccgaacgg cgccagagcc gcaggggttt
     5281 ggactcggct ggtaatttcc ggtggagtcg tgctgttgtg gaatgtaaac ggtctggggg
     5341 cgcgttctat gccggagaag ttctgtttcg cctcaaccgc cggcctggct gcccccgcgg
     5401 atgaactcct cgtgcaatcg ggagtaaacg ccgcccgctg ccagcagcgt ggtgtgggtg
     5461 ccgcgctcga caatgcggcc ctggtccatg accagcacca ggccggcctt gcggatcgtg
     5521 ctgagccggt gggcgacgac gaaggccgtg cgcccgcgca gaagaatctc cagcgcgcgt
     5581 tgcagccgtg cttccgtgac gctatcaatc gcgctggtcg cttcgtcgag caccacgatg
     5641 cgtgggtctg ccagcagcgc ccgggcgaag caaatcagtt ggcgttggcc cagggagagc
     5701 gccgcgcttt tctcacccac ctgcgtctcc aacccgcggg gcagcgcttc gaccaggtcc
     5761 agacagttga gcgcccgcag cgtggcgcgc acgtccgcct cgctcgtctc gggccgcgcg
     5821 aagcggatgt tgtcgctgat cgagccggcg aagaggaagt tgttctgttg cacgctcccc
     5881 atttgcgagt gcagcgagtc gctggtgacc tcgatcaggt catggccgtc caccagcacg
     5941 cggccgcgcg tcggcaggta gagcttttgc agcagaccca cgagcgtcgt cttgccgctg
     6001 ccggtgtggc cgaccagcgc gaccgtttgc ccgggttcgg cgacaaacgt gatatcggcc
     6061 agcaccggtc gcccgcgctc atattcgaag tgcacctcct ggaactcgac acgtccctga
     6121 atcggcggca gggctttggc ggtgagcgcg tcctgccact cgggttccag gtcaatgagc
     6181 cggaagtaac gctcggcgcc cgccatggcg gagagggcct ggttatactg attgccgatg
     6241 acctggacgg ggtcgaagaa gaagttggcg aggaagaaaa acatcaccag gtcgcccact
     6301 tccatgtgca gccacccgtg ccagcggagc gcgccgtaac cgctgagcag cgccaccgcg
     6361 ccgaggaaaa gctggctctt catctggagc aggggaatga acaccgccga ggcgcgggcg
     6421 acgccgacgt tgttttcccc gtgcaagttc accagcttgc ggaagaagcc ggcgttgatc
     6481 tcctgtcgca caaacgcctg cgtgacccgg atgccgctca ccgattcggc cagcgtggaa
     6541 gtcagccggc tccaggtctc ctgcagcttg cgcaactggt ggctcacttt ccgccggtag
     6601 ttcaggttga ccacccatat ccccggcacc agcagcagca tcagcgaaaa cagcttccag
     6661 ttgtaccagg ccatcagcag ggccgcgacc gtcatctgca ccgcctggat cgtcaccaca
     6721 aacgccacgt cctgcacggc cacgcggacg ctgtcaatgt cggacgtcat ccggctgatg
     6781 atgcggccaa acttggtctt gttgaaaaac gacattggca gcgtcgccag ctttcggaac
     6841 agctccaggc gcatgtcatg ggcgacggcc tcgcccagtt cgagggcgaa acgctggcga
     6901 aagtgcagcg tcaccaccat cagcagcacc agcaccaaat agatcccggc atggaggtaa
     6961 atggccggca ggttccgtcc cgcaatcggg ccattgatcg tctggccgat catccacgcc
     7021 agcgcgggta actgcaagcc gcgcgcgaag gtcaggacaa acagccagtt gcgccgtgcc
     7081 gtataccgcc gggtgtaggt gaacatgcgg cggatgagtt cggcgctgag cggcgcgtgg
     7141 tcggcctccc gctcctcgat gggccggaag tacgtgatcg cctcgcgtgc ggtgccgagc
     7201 ttgtctgcgg ctggcgctat cggtgacttg ctggttgggg acacgctcat ggtccttggt
     7261 ccctcccttg ttcttccgtg agatccatca gttggagcag cgccgtttcg cggtatgtcc
     7321 ccggcacatg cgcgagttcg tcatgggtgc ctacatgcag gagccgtccc ttttccagga
     7381 ccagaatgac atcggcccgg cgcaacaggc tcagacgatt cgccaccacg aatgtcgtgc
     7441 gccctgccat cgcctggcgc agggccgaga cgatttcatg ctccgtcttg gggtccaccg
     7501 aggcggtcgg gtcgtccagg atcaggattg gcggctccag gagcagtgcg cgcgccagtg
     7561 ccagccgctg ccgctggccg ccggagaggt ccacccccga ctccccgagc accgtttcgt
     7621 agccgtgcgg catggccaca ataaactcat gtgccgaagc ggtgcgggcg gcccgctcga
     7681 tttgctcctg cgtggcgtgc gggttcccga acgcgatgtt ggccgccacc gtattcgaga
     7741 agagaaagct ctcctgatag acaatgccga tctggcgccg gtaagcatcc aggtcgaggt
     7801 cccgcagatc ccgcccatcc gccaggatgc gaccgtgctg gggatcatag aatcgcggga
     7861 taaggctgag cagggaactc ttgcctgcgc cagtcatgcc gaagatgcca atcacctgac
     7921 cgggcgccgc ttcaaacgac acgtcctgca acacgctcgc ctccggcacg tagccaaacg
     7981 tcacgccttc aaacgccagc cgccccgtca atcgccccgg cacgatggcg ttcggccggt
     8041 tctggacctc tgaaggcgtg tcgagcacct cgaacacgcg ccgggctgcc gtgaaactct
     8101 gctgcaccga gttggcgatg gttgtgatat tggccacctg cccgttgaac tgctgcaaga
     8161 ggccggcgaa caccaccagc ccgctgccca gcggaatccg gccctgcacg taaagccacc
     8221 cgccataggc gaacaggatg accaggctca cttgcgacaa cacctgcgtc agcggcgtga
     8281 agtttgacag gtcgcggaaa atccgctttt gctgatccga gacccgcgcg ttggcctcct
     8341 cgaagcgccg gatttgatgc ggctccgccg caaagccctt aaccgtttgc atcccgcgga
     8401 tgctttccgt gaaggtcagc accaggctgt cgtatagctc gcggttgcgc aggtaggcgg
     8461 gccgcagtcg gcccgagtag tagtgggtga cccaccaaag tccgcccgtg accgacaggc
     8521 acgcgagcgt cagtgacggc tgaatccgcc acatgaacac gaaataggcc gccaacgtca
     8581 acagcatgtt gaccccttgc aatactacgc catccacgaa caggcgggta ttctgcacgt
     8641 cccccgtcac ccggttaaag atcgagctgg agccgtgcac gtcgaagaag cggaagctca
     8701 gccgttgcag cttgtcgtac acccggtcgc gcaggtcggg gacgatcttc ccctgcgtca
     8761 gcagcgcggt ggtcatattg tagtggtagg tcagaaacgc ccgcagcacc gcctgcgcga
     8821 caatcgccag cgccatcact gtgacgattg tcagtggagt ccaccccgcc gggggacccc
     8881 agccaaacgg atacaccggc gcccgctgcg tcgggtcgag cgcgtgacgg atgacatcaa
     8941 tgaccacccc cagcaactgg agccccaaca ggccgagcaa cagcagcatc acgctcagca
     9001 ccagcgaccg caggcaatcc ccgcggaact gccacgccag cccaagcagc cgacgcacca
     9061 gttcgccggt ggcataccgg cgctcgtagt gcttgttggg cggtgaatcg gacatactca
     9121 gcctgacaac atgccgcctc ttccgccgcc aatccagagg aaacccttaa cgcggcgcaa
     9181 gcgtctcgcc tgcgagctca ccgggcatcc cgcccagtga gatgcgatcg attgacgccg
     9241 ggacgccgcc caaacccgca cctgggaagc gtggcccagg ccacgagcgc caccattccc
     9301 gctgaggagg gatgggatga cgcgtagcaa accgcggaaa ccctagcgcc gcccatgcga
     9361 agtctatgcg aggcccatgc gaagcccatg cgaaagatgc cgcgtcttcc ccctgtcccc
     9421 tgcccagcaa caccgtgttc ggcggccctt cgttccggct aattgccaga ccacttgtag
     9481 tcatatccag caggaggctc gtaaaaggca gagatgccat cgaatccttt gacaccgttc
     9541 caataaatgc ggatgtagct gacgtgccca tccacaaagc ccattacgtt cggcgcattg
     9601 ttgaactgat agggttcctt gcggtcgtgg gcttagcccg caggcaccgg aaagctcgcc
     9661 cacgagcacg acgcgggaag gctcccgtag agagcttcct tgttcgtgac ggcacggatg
     9721 gcgtcctggt gatcgtccgc atacatgcgc agtgccagat taatctggcg agcgttgctg
     9781 atgcaagttg tcctcctggc agcatctctg gcccgactga gagccggtag aagca
//
LOCUS       DATCGS010000461         3188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_336558, whole genome shotgun sequence.
ACCESSION   DATCGS010000461 DATCGS010000000
VERSION     DATCGS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336558"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1896
                     /locus_tag="VIL39_09485"
     CDS             148..1896
                     /locus_tag="VIL39_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5481698.1"
                     /translation="MPESLSLDRPQKEGRRGVYLAELRSSSERATIKRFMRLQKWGVW
                     EHLDEGKDLLQSIKESEDYTDYWLDRRLGCRQLGMNLTRRVAMRRLNEIYNGTNARFR
                     GELIRTIYFDREYLPGIATDKLTLEKYAQPGYGPKLASLLGRAAATSMIVGRALESGT
                     RPAFDDGDEVVREGEDRLPVEIMIGDHSGAFGDYKLPLEAFAAHYARPVNTRDKFVPD
                     PKGFATAYLDAFREQFLHIQGDYRKRRRAFNTLFKHCNYDSGGSFAFRWDCVLRRLDQ
                     TNPHTLVEAIRQQIWVLNREPAKLSNSFAGKVIFSPSFAPRPSVKHVLFDFDGTLSLI
                     REGWPAVMLGMFLEMLPRRTGEEVEDRKMLWDDMMRLNGKQTIYQMMQFADRIRERGG
                     QPQEPLWYKREYLRRLDEKIAARREAIRTGAVSRDKYLVCGSIGLLEELRRRDLRIYL
                     ASGTDEPFVKAEAELLGMVPYFGSHVYGALDDYKKFSKKMVIERILRENQVLGEQLLA
                     LGDGYVEIQNTKEVGGLAVAVASDEANNGSGEFDAWKRNRLLEVGADVVIPDFRDAVP
                     LLQCILDGAPRPDIAS"
     gene            1913..2254
                     /locus_tag="VIL39_09490"
     CDS             1913..2254
                     /locus_tag="VIL39_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481699.1"
                     /translation="MLSSFANALTALKERAMEATVKRLVNQKLETFGSVTSLRIDSKQ
                     RTISAQVSLKGETQPIEIKAGSYEVIQENGAAYISFQNLHASKEWIGNLLNEYVAGRR
                     FKVPDAVGLVL"
     gene            2424..2657
                     /locus_tag="VIL39_09495"
     CDS             2424..2657
                     /locus_tag="VIL39_09495"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEY5481700.1"
                     /translation="MEATEREDREKHELRARLEAATEKAKEVCKKLQEETVAAAKATD
                     KAVREHPYQAIGIGFGLGLLIGVLVMRCRCRHD"
     gene            2922..>3188
                     /locus_tag="VIL39_09500"
     CDS             2922..>3188
                     /locus_tag="VIL39_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481701.1"
                     /translation="MKVEPWLPATRFDLPVGNLAARSTQTSLTPATNRLGDAERRPHG
                     TKILPFEEAQHERVAVRWIQLVDDEGFGRVRPLRPQVLLPASFRN"
BASE COUNT          686 a          899 c          974 g          629 t
ORIGIN      
        1 tagttcctct ttgactcgat cttcgatgaa gcggcgaatc accccgaaga cgcggaactg
       61 cagcggcttt gcgatccgcg ggccaagggc atcatcttca atcttatccg cgagtacggc
      121 gacctcgact tcgttaacgt ggggtgtgtg ccggaatcgt tatcgctcga ccggccgcag
      181 aaggagggcc ggcgcggcgt ctatcttgcg gaactccgtt cgtcgagcga gcgcgctacc
      241 atcaagcgct tcatgcgcct ccagaaatgg ggggtgtggg agcacctgga cgaaggcaag
      301 gacctgctgc aatcaatcaa ggaaagtgaa gactacaccg actactggtt ggaccggcgg
      361 ctgggctgcc gccagttggg gatgaacctg acccgccgtg tcgccatgcg ccgtctgaac
      421 gagatctata acggcaccaa cgcccggttc cggggcgagc tgatccgaac catctacttc
      481 gaccgtgagt atctgccggg cattgccacc gacaagctga cgctggaaaa gtatgcccaa
      541 cccggctacg gccccaagct ggcgagtctc ctcggccggg cggctgctac gagcatgatc
      601 gtcgggcgtg cgctcgaatc cggcacccgt ccggctttcg atgatggtga tgaagtggtg
      661 cgcgagggcg aggacaggct cccggtggaa atcatgattg gcgatcacag cggcgccttc
      721 ggggattaca agctccccct ggaagccttc gccgcccatt atgcccggcc ggtcaatacc
      781 cgcgacaagt tcgtgcccga tccgaaagga tttgccaccg cttacctgga tgccttccgc
      841 gagcagttcc tgcacatcca gggcgactac cgtaagcgcc gccgcgcgtt caacaccctg
      901 ttcaagcatt gcaattacga ctccggcggc agcttcgcct tccgttggga ttgcgtcctg
      961 cgccgtctgg accagacgaa cccccacacg cttgtcgaag cgattcgcca gcagatctgg
     1021 gtgctgaacc gcgagccggc gaaactgtcc aactcttttg ccggcaaggt aatattcagc
     1081 ccttcgttcg cgccgcgacc ttcagtcaaa cacgtcttgt tcgactttga tggcacactg
     1141 tccttgatcc gggagggctg gccggcggtc atgctgggca tgtttctcga aatgctgccc
     1201 cggcgcacag gcgaggaggt agaagaccga aagatgctct gggatgacat gatgcgtctg
     1261 aatggaaagc agacgatcta ccagatgatg cagtttgccg accgcatccg ggaaaggggc
     1321 ggccagccgc aagaaccact gtggtacaag cgtgagtatc tccgcagatt ggatgagaag
     1381 atagcggcgc gccgtgaagc gatccgcacc ggggcggtga gccgggacaa atacctggtc
     1441 tgcggctcga tcggcctgct ggaggaactg cggcggcgcg atctgcgtat ctacctggcc
     1501 agcggcaccg acgaaccatt tgtaaaggcc gaagccgagc tgttggggat ggtgccgtat
     1561 ttcggaagcc atgtttatgg cgcgctggat gactacaaga agttctccaa gaagatggtc
     1621 atcgagcgca tcctccgtga gaaccaggtt ctcggggagc aactgctggc tctgggtgac
     1681 ggctatgtcg aaatccagaa tacgaaggag gttgggggcc tggccgtggc cgtggccagc
     1741 gatgaagcca acaacggttc cggcgaattc gacgcctgga agcgcaaccg gctgctggag
     1801 gtgggcgccg atgtcgttat cccggatttc cgggatgcgg tgcctttgct ccagtgcatt
     1861 ttggacgggg caccccgccc ggatatagct tcttagctac gattgacgat ttatgctcag
     1921 ctcattcgct aacgccctaa ccgccttaaa ggaacgggcc atggaggcaa ccgtaaagag
     1981 gctcgtcaac cagaaactcg aaacctttgg cagcgtcacc agtcttcgca ttgactcaaa
     2041 gcaaaggacg atctctgcac aagtgtcttt gaaaggtgag acacaaccca tcgaaatcaa
     2101 ggccggctct tacgaagtga ttcaggaaaa tggggcggct tacatctcat ttcagaatct
     2161 ccatgcctcc aaagagtgga ttggcaacct tctcaatgag tatgttgccg gtcgaagatt
     2221 caaggtgcct gatgcggttg gactggtctt gtaagcttcg atgcatcaca gcttcacttc
     2281 aggagccttt tcgtatgccg agtgccgagg gttagagctc agcaagtttg gtcaccgttc
     2341 tgatggcgag ggccattccg cagaaactgc tattgcgaag gcccggcaat ggtgcgagag
     2401 tgaatcaaga aaggaagaaa tctatggaag caacagaacg tgaagacaga gagaagcacg
     2461 aactccgagc cagactcgag gccgcaaccg agaaggccaa ggaggtttgc aagaagctgc
     2521 aagaagagac cgtcgccgcc gccaaggcca ccgacaaggc tgtccgcgag cacccgtatc
     2581 aggccatcgg aatcgggttc ggcctgggcc tgctcatcgg cgtgctggta atgcggtgca
     2641 ggtgcagaca cgactgagat cgaatcggtt tgaaacccct tgtcagcgag aagggcgcgc
     2701 ttttggattc agccacggcc cgaatccagg aagcgtgtgc cttaacatcg tagtgcttgg
     2761 tggccccagc atgcacgtgc attgggaggc cacgatgata tgaggagtaa gcggaatatc
     2821 aacgcatcat ctgggcgctg ctggagacgc ggcggttgat gggcgagatt gacgccagca
     2881 ttaaccagca cggcggctgg ccgctgaagt agatgaggtt cttgaaggtg gaaccctggc
     2941 ttccggcaac ccgctttgat ttgcctgtcg gcaacttggc tgctcgttcc actcaaacct
     3001 cactaacgcc tgccaccaat cgcctcggag atgccgagcg ccgcccccac ggcacgaaga
     3061 ttctgccgtt tgaggaagcg caacacgagc gcgtcgcggt ccgctggatt cagttggttg
     3121 atgatgaggg attcggtcgc gtgcgtccgc ttcggccgca ggttctcctc ccggcctcat
     3181 ttcgcaat
//
LOCUS       DATCGS010000462         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_189270, whole genome shotgun sequence.
ACCESSION   DATCGS010000462 DATCGS010000000
VERSION     DATCGS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189270"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VIL39_09505"
     CDS             complement(<1..539)
                     /locus_tag="VIL39_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY5481702.1"
                     /translation="MPVQYKDYYETLGVPRSASDIELKKAFRKLARVYHPDVAKNKKQ
                     AEEKFKEINEAYEVLGDSAKRKRYDELGANWQSGADFRPPPGWESYGQGRASQGRYAG
                     GGGQEFHFGGTGFSDFFEQIFGSKGGRGEAGFGRGGGFGGEEMGAERGQDMEGDILVT
                     LEEAMHGSVRSVSVRHGVA"
     gene            complement(795..2297)
                     /locus_tag="VIL39_09510"
     CDS             complement(795..2297)
                     /locus_tag="VIL39_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5481703.1"
                     /translation="MRSAALGGLSILIAFQAASLGGAAPASAKTNPAPRLTIQGTPIN
                     RDVKAATSVAPVVKKVAPSVVNIYSTVTIRERPNPHLSDPFLRRFFGEDFGGQTQPRT
                     HREQSLGSGVIVSPNGYVLTASHVVEGADKVKVALASGEQEFDAKVIGTDPATDTAVL
                     KVDAKNLPAITIADSDKLEVGDVVLAVGNPYGVGQTVTMGIVSALGRGGFGINNYENF
                     IQTDAAINVGNSGGPLVDAEGRLVGINTWIISGSGGSQGLGFAVPINLARYAMERLIS
                     DGKVARGYLGLALQQDMTPELAKQLNLPNVNGALVTAVEPDSPAGKAGFKEADFVIEF
                     NGQKVKDMRQLRLMVSQTAPGKKVTLIIVREGKEKTLTATLGEIPRDVLARSGKAQPG
                     ERGESKADALDGVEVSDLDAEARREGDIPGNVRGVLVTKVDQDSNAAEAGLRAGDVIN
                     EINRQPVRGADEAVALSEKTKGDRLLLRVWRGGEDRSGMLFLSVDNTKRK"
BASE COUNT          449 a          901 c          775 g          555 t
ORIGIN      
        1 caggccaccc cgtgccgcac ggagactgac cggaccgaac cgtgcatggc ttcttccagc
       61 gtcaccagga tgtcgccttc catgtcctgg ccgcgttccg cgcccatttc ttctccgcca
      121 aagccgccgc cgcgcccaaa ccccgcctcg ccccggccac ccttcgagcc gaagatttgc
      181 tcgaagaaat cgctgaagcc ggtgccaccg aagtggaatt cctggccccc gccgccggca
      241 taacggccct gggaagcacg gccttgccca tacgattccc agccgggcgg gggacggaaa
      301 tcggcgccag actgccaatt ggcccccagt tcgtcgtagc gcttgcgttt ggccgagtcg
      361 cccaggactt cgtaggcctc gttgatctcc ttgaacttct cctctgcctg cttcttgttc
      421 ttggcgacgt cggggtggta cacgcgcgcc agcttgcgga acgccttctt gagttcgatg
      481 tcgctggcgc tgcggggcac acccaaggtt tcgtagtaat ctttatattg cacgggcata
      541 actgtggcgg ttaattacgg cacaaattgc cgtcctgttc cagacaaaat gcccggcgcc
      601 gggcatttgt ccagtgctgt tggcaatgta ggcgtctcgc ctgcgggttt agccagcgtc
      661 ccgtcgccag tgttcgtagc gttaccgggc gggtcgcagg cgagccgcct gcgccacacg
      721 atcaagcctt ccggctggcc acctgtttcc agtcgctctc gcgccgcaag gcggacttgc
      781 ctgacgcgca gcgcttactt ccgtttggtg ttgtccaccg agagaaagag cattccgctc
      841 cgatcttcgc cgccgcgcca gacgcgcaga agcaggcggt cgcctttggt cttctcgctc
      901 aaggccaccg cctcatccgc gccgcggact ggctggcggt tgatttcgtt gatgacgtcg
      961 cccgcgcgca agccggcctc cgccgcgttg gaatcctgat ccaccttggt aaccagcaca
     1021 ccccgaacgt tgccggggat gtcaccctcg cgccgcgcct cggcatcgag atcagatact
     1081 tccacgccat ccagcgcgtc ggccttcgac tcgccgcgtt cgcccggctg ggccttgccg
     1141 ctacgcgcca acacgtccct gggtatctcg cccagggtgg cggtcagagt cttctctttg
     1201 ccctcccgca cgatgatcaa cgtcactttc ttgcccggag cggtctggga aaccatcagg
     1261 cgcagttgcc gcatgtcctt gaccttctgc ccgttgaact cgatgacgaa atcggcttcc
     1321 ttgaacccgg ccttcccggc cggcgaatcc ggctccactg cggtcaccag ggcgccgttc
     1381 acattcggca ggttgagctg tttggccagc tcgggcgtca tgtcctgctg cagcgccagg
     1441 cccagatagc cgcgcgcgac cttgccgtcg ctgatgaggc gttccatggc gtaacgcgcc
     1501 aggttaatcg gcacagcgaa tccgaggccc tgcgagccgc cgctgccgct gataatccag
     1561 gtgttgatgc ccaccagccg cccttcggca tccaccagcg gcccgccgga attgcccacg
     1621 ttaatcgccg catcggtctg gataaagttc tcatagttgt taatgccaaa gccgccgcgt
     1681 ccgagggcac tgacgattcc catcgtgacg gtctggccaa cgccatacgg gttgccgacg
     1741 gccagcacca cgtcgcccac ttccagtttg tcactatcgg caatcgtaat cgccggcagg
     1801 ttcttcgcgt ccaccttcag caccgccgtg tccgtggccg gatcggtccc gatgaccttc
     1861 gcgtcgaact cctgctcacc cgaagcaagc gcgaccttca ccttatctgc gccctcaacg
     1921 acatggctgg cggtgaggac gtagccgttg ggtgaaacga tcaccccgga gccgaggctt
     1981 tgttcccggt gcgtgcgcgg ctgggtctgg cctccgaagt cttcgccgaa aaagcggcgc
     2041 aagaacgggt cgctcaagtg cggattgggc cgctcgcgaa tcgtcaccgt ggaatagata
     2101 ttcaccacgc tgggcgcgac cttcttgacc acgggcgcca cgctcgtcgc ggctttgacg
     2161 tcgcggttga tgggagttcc ctggatggtc aagcggggag cgggatttgt tttggctgac
     2221 gccggggcgg cgccgcccag cgaagctgcc tggaaggcga taagaattga gaggccgccc
     2281 agcgcagccg accgcatagt gaaaagaaat tccgaaatcc tgctcatgtc gtttagttat
     2341 cctatgtttc tgcattacgg cagcaatgta actttcactt gcgtgacgcc tggtttgacc
     2401 cttcgtgctg ccacctgtcc aaaagtcaca ccacccgcgg cctttgtcaa aaagggagtg
     2461 tggactcaac gctgaatcat cagcgccttt gctaggggcg aggctacctc gatgctggat
     2521 ctcacacggg aaactaaccc gagtgccggg ccttcgccac ccggcgggcg gcagtatggc
     2581 cttaactatt gaaggggccc ggagatcttc cactcattgc cgatcttctt cagttccttg
     2641 gacaatcccg cctgtgcacc ggcacggtga attggtcccc
//
LOCUS       DATCGS010000463         6666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_633572, whole genome shotgun sequence.
ACCESSION   DATCGS010000463 DATCGS010000000
VERSION     DATCGS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633572"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VIL39_09515"
     CDS             <1..994
                     /locus_tag="VIL39_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5481704.1"
                     /translation="RRERKGDMQLSQSNRRAFIRKSFAVAATAALFEPGSVLLGRASA
                     AQPRLQPIRLGGPSFAGTEDPEALALAHRKLGYRAAYCPGVSLKDGDRIRALAEAFAK
                     HDVVIAEVGRWSNLLDADPEKRRKNLETVTEGLALAEAIGARCCVDIAGSFSSTSWFG
                     PHPENLSPKHFDAAVENARKIIDAVKPKRARFCYEMMGWALPDSPDSYVKMIKAVHRP
                     AFGVHLDPCNLVNSPDKFYHNTALLNECFNKLGRWIVSCHAKDLTWDLEMNVHFREVA
                     AGKGSLDYSTYLKRVAELPQNPPLMLEHLPAAEDYAGAREHIFEVGRKTGLSFG"
     gene            1039..1959
                     /locus_tag="VIL39_09520"
     CDS             1039..1959
                     /locus_tag="VIL39_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5481705.1"
                     /translation="MNSAIGNQEKNRAALNSVAAAFLLTGLKVVVGLFSGSMGILAEA
                     AHSGLDLAAAIVTCFAVRAATKPPDRDHAYGHGKVENLSALVETLLLLATCVWIINES
                     FHRLTSHRGQVDASIWAFAVMGISIVVDFSRSRMLYRVAGKHRSQALEADALHFSTDI
                     WSSAVVIFGLLGVKLAGWYPSLSFLLQADAVAALMVAGIVVMVCGRLGVRTIQALLDS
                     SPPGMDEKVRAAVAAMDGVFDCHAVRVRHSGPYYFVDLHITVDGEQSLRAAHALTERV
                     EQAVEAILPGVDVTVHPEPSPVVVKPPAAP"
     gene            2149..4110
                     /locus_tag="VIL39_09525"
     CDS             2149..4110
                     /locus_tag="VIL39_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481706.1"
                     /translation="MLSALGQLNEAGYAVFCVVGVSGLWFGRKALGLTSPRDLCNWWK
                     VRARFRRWLPASFVVLAFLVVLGGALYPPTNHTAMTYRTPRVLHWLAEGHWHWIHTPN
                     YRMNDRCCGFEWLTAPMLLFTKTDRCLFLINFISFLLMPGLTFGVLRRLGVRPRVAWP
                     WMWLLPTGYNFLLQAGSIGNDTFPTIYALAAIDFGLRAWESRRLSDLWLSILSAALLT
                     GTKATNLPLLLPWGILVLGILPWLLGRPAFGGARTFLSAAASKRRGDPDTLPGSAEAH
                     RCGQECPRAGLGTYAATLALVLLAATVSFLPTAALNIRYCGDWSGLSLEKTGMDMKSP
                     IVGVWGNGLLLLKNFVPPIFPLAGWWNHSARSIVPHAIISRMDASFEEGYLGLGELPI
                     EDTCGIGFGFSWLLLISVLAAWWMRSAPATGWRGNQSLPDWLRWLVLVAPWGSLLAYG
                     MKSGIVDAGRLISAYYPLLLPLLLVGAGQAVIVRCRWWRAMVLGVVLLAVPVLVLTPG
                     RPLWPARTVLSRLLAWKPDHGLLKRALNVYSVYDVRSDPLASVRALLPKGISVVGFMG
                     TPDDIDISLWRPFGERRVEHILLSDSREQIRKRHIEYAVVGEVNLLESQTTLGAWQAK
                     TGAQVVAMATATMTVTQGPHPWYIVGFPD"
     gene            complement(4135..4791)
                     /locus_tag="VIL39_09530"
     CDS             complement(4135..4791)
                     /locus_tag="VIL39_09530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5481707.1"
                     /translation="MNWTYLRSCAWVDTRARFVAGTPAGGTLLDLGSSDGQTLGHMAE
                     LRPDLHFFATDMAGTPEHYPAGCQFQRADLEQDRLPWAAGSMDAVTCMHLVEHLRDLT
                     WLLQEAARLLKPGGRIYFETPHPKTLTLASPRGRAAGTFTLNFFDDPTHVRLVALGAL
                     AQQVRGVGLEVAASGTSRSWLLAAAYPLFVFLPASQQKFTARAHWLGWSAYLIARRPL
                     "
     gene            4972..6447
                     /locus_tag="VIL39_09535"
     CDS             4972..6447
                     /locus_tag="VIL39_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY5481708.1"
                     /translation="MIWSPATSAARRNLAIALTLLLTLVALTLRFYRISNQSLWTDEV
                     SSVETAREPLSQMTQDSGSENVYLPGYFLLLGAVLSDASRDIEFRARGLSALAGALSV
                     PLFIGLVYCWRRRWGTALLAGLLLTVNPLHLWYSQEVRAYATMLFFGLLTLLAYELAR
                     ASRKPWWWVVYFLSALGAIGLHKTAIIFPVVCALWHGWEVWREKGRLRDLAIHVVTLG
                     VVCGAVLFRQYPPPPVNGRSRSILEVGYTFMTFVGGYSFGPSITDIQSYGALGAVSRN
                     LVQVAILGGVLALVALAYALNLRSLVRGKETSLVVLNLAFVSLTTLAFAFPFNVRYTL
                     PGLLAFVALVAALNGGAGRRYVARLAIVAVLLLALWADAQWFYRPAYRKTDSRAMAQW
                     LVANQVRVKSWTVLPDYLSVSVAWYLHGHPEILARKQPPTQAQTTSFPPVPDALIIGR
                     RHHVTEPDKIIAAYRASAGPVRAIPAIAGFEIYVREAAAPE"
BASE COUNT         1067 a         2041 c         2219 g         1339 t
ORIGIN      
        1 ccggcgtgag agaaaagggg acatgcagct tagccaatcc aatcgtcgcg cctttatccg
       61 caagtccttc gcggtggccg ccacggcggc gctattcgag cccggctcgg tgctgctcgg
      121 tcgggcgtct gccgcacagc ccaggttgca gcccatccgg ttgggcggcc cttccttcgc
      181 cggcactgag gatcccgagg cgctggcgct cgcgcatcgc aagcttggct accgggctgc
      241 ctattgccca ggcgtgtcgc tcaaggacgg agaccgaatc cgcgcgctgg ccgaggcgtt
      301 tgccaagcac gacgtggtca tcgccgaagt gggccgctgg tccaacctgc tggatgccga
      361 tccggagaag cggcgcaaga acctggagac ggtgacggag ggactcgccc tggccgaagc
      421 cattggcgcc cggtgctgcg tggatatagc gggctcgttc agctcgacct cgtggttcgg
      481 gccgcacccg gagaacctct cgccgaagca cttcgatgcc gccgtggaga atgcccgcaa
      541 gatcattgac gcagtgaaac ccaagcgcgc ccggttttgc tacgagatga tgggctgggc
      601 cttgccggac agcccggaca gttacgtgaa gatgatcaaa gcggtgcacc ggcccgcctt
      661 cggggtgcac ctggacccgt gcaacctggt taactcgccg gacaagttct atcacaacac
      721 ggccctgctc aatgagtgct tcaacaagct gggccgctgg atcgtaagct gccacgccaa
      781 ggatttgacc tgggacctgg agatgaatgt gcatttccgg gaagtggcgg ccggcaaggg
      841 ctccctggac tactcgactt acctgaaacg cgtggcggag ctgccgcaga atccgccctt
      901 gatgctggag catcttcccg cggccgaaga ctacgcggga gcgagggaac atatcttcga
      961 ggttgggcgc aagactgggt taagctttgg ctgaatgaca ggcagaggaa ctcagccacc
     1021 gctcgcggca ccgcgcacat gaactcagcc atcggcaatc aagagaagaa ccgggcggca
     1081 ctgaattcag tcgctgccgc cttcctgctg accggtctga aagtggtggt tggcttgttc
     1141 agcggcagca tgggtattct cgcggaggcg gcccactcag ggctggatct tgcggcagcc
     1201 attgtaactt gctttgcggt gcgagcggcc accaagcccc ctgaccggga ccacgcctac
     1261 gggcatggca aagtggagaa tctctcggcg ctggtcgaaa ccctcctgct gctggcaacc
     1321 tgcgtttgga tcatcaacga atcctttcat cggctgacct cccatcgcgg ccaggtggac
     1381 gcttccatct gggcctttgc cgtcatgggc atctcgatcg tggtggattt ctcgcgttcc
     1441 cggatgctct accgcgtggc gggcaaacac cgcagccagg cgctcgaagc cgacgccctg
     1501 catttcagca ccgacatttg gagttcggcc gtggtgatct tcgggttgct gggagtcaag
     1561 ctcgccgggt ggtacccgag cttgagtttc ctgctccagg cggacgccgt ggcggcgctg
     1621 atggtggcgg gcattgtggt gatggtctgt ggccggcttg gcgtgcgcac gatccaggcg
     1681 ctcctggatt cctcgccccc gggaatggac gagaaagtca gggcggccgt tgcggccatg
     1741 gacggggtgt tcgactgtca tgccgtgcgg gttcgccatt cgggccccta ctactttgtt
     1801 gatttgcaca tcaccgtaga tggcgaacaa tcgctgcggg ccgcccacgc cctaaccgag
     1861 cgagtcgagc aggcggtgga ggcgatcctt cccggggttg atgtaaccgt ccatcccgaa
     1921 ccgtcgcccg tcgtggtcaa acctccggct gctccatagc gccatcaacc aagtgccgcg
     1981 caccgccagg atcttgtaca ggaattgact ggggtgggga atccgcctac tgtccggcct
     2041 gcggtcgtta gtgactggca agcgccgatt gttcaaacgc agatgtcttt tctcccggtg
     2101 gtcagagtct ggctttgggg ttcagtcctc gcaagcgttg cggggtgggt gctttctgcc
     2161 ctgggccaac tgaacgaggc cggctatgcg gtcttctgcg tcgtgggagt ttccgggctt
     2221 tggtttgggc gcaaggccct gggattaact tcaccgcggg atctctgcaa ttggtggaaa
     2281 gtccgggcgc ggtttcgccg gtggctgccg gcatcctttg tggtgctggc gttcctggtc
     2341 gtgctcgggg gcgcgcttta cccgccgacc aaccacacgg cgatgaccta tcggacgccc
     2401 cgcgtgctgc attggctggc agaggggcat tggcactgga ttcacacccc gaattaccgg
     2461 atgaacgacc ggtgttgcgg cttcgaatgg ctgaccgctc ccatgctgct cttcacgaag
     2521 acggatcgct gcctgttcct gatcaacttc atctcgttcc tgctcatgcc gggcctgacc
     2581 ttcggcgtgt tgcggcggct gggggtgcgg ccgcgggtgg cgtggccctg gatgtggctg
     2641 ttgccgacgg gctacaactt tctgctccag gccggcagca tcggcaacga caccttcccc
     2701 accatctatg ccctggcggc gattgatttc ggtttgcgcg cctgggaatc gcgccggctc
     2761 tccgatctct ggctgtccat cctctcagcc gccctgttga ccgggaccaa ggcgacgaac
     2821 ctgccattgc tcctgccatg ggggatcctc gtgctgggaa tactcccctg gttactgggc
     2881 cggccggcgt ttggcggcgc gcggacattc ctgtccgcag cagcttccaa acggcggggc
     2941 gaccccgaca cattgccagg ctcggctgaa gcacatcgtt gcggacagga atgtccgcgc
     3001 gccgggctag gaacgtacgc ggccacgctg gcgctggtgc tgctggccgc caccgtctcg
     3061 tttctcccca ctgcggcgct gaatatccgt tactgcggcg actggtcggg cttgagcctg
     3121 gagaagacgg ggatggatat gaagagcccg attgtgggcg tctgggggaa cggcttgctg
     3181 ctgctgaaga acttcgtgcc ccccatcttc ccgctcgcgg gctggtggaa ccattcggcg
     3241 cggtcaatcg tgccgcacgc gatcatcagc cgcatggatg cgagtttcga ggaggggtat
     3301 ctcgggctgg gcgagttgcc gatcgaggac acctgcggca tcggcttcgg gttcagttgg
     3361 ctcctgctga tctccgtgct ggccgcctgg tggatgcggt ctgccccggc aacgggctgg
     3421 cgcggcaacc agtccctgcc cgactggctg cggtggctgg tgctcgtggc gccgtggggt
     3481 tcgctgctgg cctacggcat gaagtcaggc atcgtggacg cggggcgcct catctcggcg
     3541 tattacccgt tgctgctgcc attgctgctg gtgggcgcgg ggcaggcggt gatcgtccgg
     3601 tgccgctggt ggcgggcgat ggttcttggg gtggtactgc tggccgtgcc ggtgctggtg
     3661 ctgacgccag gacggccgct gtggccggcg cgcaccgtgt tgtcgaggct gctggcatgg
     3721 aagcccgacc acggcctgct gaagcgcgcc ttgaacgttt atagcgtcta tgatgtgcgc
     3781 tcggacccgc tggccagcgt ccgggctttg ctcccgaagg gtatctccgt agtgggcttc
     3841 atgggcacgc ccgatgacat tgacatctcg ctgtggcggc ccttcggcga aaggcgggtg
     3901 gaacatatcc tcctgagcga ttcccgggag caaatccgca aacgccatat agaatatgcc
     3961 gtggtcgggg aagtgaacct gctggagagc cagacaacct tgggcgcctg gcaggcgaag
     4021 accggggccc aagtcgtcgc gatggccacc gcgacgatga cggttacgca agggccgcac
     4081 ccgtggtaca tcgtcgggtt ccccgattga gcgccaacag gtgccttgcc tgtcctacaa
     4141 cgggcggcga gcgatgagat aggcggacca gccaagccaa tgagcgcgcg cggtgaattt
     4201 ctgctggctg gccggcagga agacgaacag cggataagcc gccgcaagga gccaactccg
     4261 cgaggtcccg ctggccgcga cttccagccc aacgccgcgc acctgctggg ccagcgcgcc
     4321 cagcgctacc agccgcacgt gggttggatc gtcaaagaag ttcagcgtga aggttccggc
     4381 ggcccggcca cgcggactgg cgagggttag cgtcttcggg tgcggggtct cgaaatagat
     4441 gcgtccgccc ggcttaagca accgcgccgc ctcctgcagc aaccaagtga gatcccgcaa
     4501 atgctcgacc aggtgcatgc acgtgacggc gtccatcgaa cccgcggccc aaggcagccg
     4561 gtcctgctcc aaatccgccc gctggaattg gcagccggcg ggataatgct ccggcgtgcc
     4621 cgccatgtcg gtggcaaaga agtgtaaatc cggccgcaat tcggccatgt ggcccagcgt
     4681 ttggccgtcc gacgagccca ggtccagcag tgtgcctccg gcgggggtgc cggcgacaaa
     4741 ccgggcgcgg gtgtccaccc aggcgcaaga tcgcaggtaa gtccagttca tggtgttctt
     4801 tggacgcgcg cggacaatcc catcacccga tcgccccagt cgtcgtcgtc gcaaccatag
     4861 ccgttatcca aatcggaaac aagcaaagca gattgccgcc gatgcgactt gcgcctctgg
     4921 aggcgctttt ggaggtttga cgccaatggg gagagggcta tgctccgggc aatgatctgg
     4981 tcacccgcca cgagcgcggc gcggcgcaac ctcgccatcg ccttgacgct cctgctcaca
     5041 ctggttgccc tcacgctgcg cttctatcgt atctcgaacc agtcgctgtg gacggacgag
     5101 gtctcttccg tcgagaccgc ccgggagccc ctgtcccaga tgacgcagga cagcgggtcg
     5161 gagaatgtct atctgccggg gtattttctg ctgctgggcg ccgtgctgag cgacgccagc
     5221 cgggatattg aattccgggc gcggggtctg tctgcgctgg caggggcgtt gtcggtgccg
     5281 ctctttatag gcctggtgta ttgctggcga cggcgatggg gcacggcgtt gttggcgggg
     5341 ctgctgctga ccgtgaatcc gctgcacctg tggtattcac aggaagtgcg ggcctatgcc
     5401 acgatgctgt tcttcggcct gctgacgctg ctggcctacg aactggctcg ggcgtcccgc
     5461 aagccgtggt ggtgggtggt ctattttctt tccgcgctgg gcgcgatcgg gctgcacaag
     5521 acggcgatca tcttcccggt agtctgcgcg ctgtggcatg gctgggaggt gtggcgcgag
     5581 aaagggcgcc tccgggacct ggcgatccat gtggtaaccc tcggcgtggt gtgcggagca
     5641 gtgctgttca ggcagtaccc gccgccgcca gtgaatggtc gctcccggtc aatcctggag
     5701 gttggctaca ccttcatgac gtttgtgggg ggctattcgt ttggcccgtc aatcacggat
     5761 attcagtcgt atggcgcgct gggggcggtt tcgcgcaacc tggtgcaggt cgccatcctg
     5821 ggcggcgtgt tggcgctggt ggcgctggct tatgcgctta atctccgctc cctcgtccga
     5881 ggcaaggaaa cgagcctggt ggttctgaac ctcgcgttcg tgtcgctgac gacgctggcc
     5941 tttgcgtttc cgttcaatgt ccgctacacg cttccggggc tgctggcgtt tgtggcgtta
     6001 gtcgccgcat tgaatggcgg tgcgggccgt cgctatgtcg cccgcctggc aatcgtggcg
     6061 gtcttgctgc tggcgttgtg ggcggacgcg caatggttct accgcccggc ctatcggaag
     6121 acggattcgc gcgcgatggc gcaatggctg gtggccaatc aggtccgcgt gaagtcgtgg
     6181 accgtcctgc ccgattatct gagcgtctcc gtcgcatggt atctgcacgg ccacccggaa
     6241 atcctcgccc gcaagcagcc gccgacccag gcgcaaacaa cctccttccc gccggtgccc
     6301 gatgcgctaa tcatcgggcg gcggcatcac gttacggagc cggacaagat aattgccgcc
     6361 tatcgcgcca gcgccgggcc ggtgcgggct attcccgcta tcgcgggctt cgagatctac
     6421 gtgcgcgagg ctgccgcgcc cgaataattg attgagcagg attggcgaat atagtatcca
     6481 ataggctcga atcaacgtgc acatggcaaa tcaaccagaa aacggcattc tcgaagactt
     6541 ccgcatccaa ttcatggaat gctggcaccg gttgccgaac aagggcctgt tccttggtct
     6601 gttggtcgcc tggctggcgc tgtttcaatt cctgggcaat tcgacgctgg gctacatctc
     6661 gacccc
//
LOCUS       DATCGS010000464         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_385144, whole genome shotgun sequence.
ACCESSION   DATCGS010000464 DATCGS010000000
VERSION     DATCGS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385144"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VIL39_09540"
     CDS             complement(<1..1469)
                     /locus_tag="VIL39_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HEY5481709.1"
                     /translation="MTTSSPVTSWPKSAQAVSAAGKAGPGGTRRREGFQLEPRLAQMK
                     QSLRDGSYQRFRTSPEVDMLAECEKEQLSWPQRAARLTRRMCEAQQVVIGPEEQIVFT
                     QTATKFPRIYSDAQMSALTLGRKLHELGPISNICADWGMVLREGLAGRRQVALATRAR
                     MKHDAEAVEFLDCAVETIDAVLNLARRYAAEAHRRGRLDIAQTLERVPAYTPRTFHEA
                     LQSLRLCHSVLWISGHYHCGLGRFDQYMWRYLQADLAAGRLDLAGAERLLAEFFLSLN
                     RDSDLYPGIQQGDNGQSLMLGGVKRDGTDGVNELTYLVLRVARGVAMIDPKINLRVTK
                     GTSLDLLELAAELTEIGLGFPQYSNDDVVIPGLVAHGYELEDARDYSVAACWEFLIPG
                     RGMEVVNIGAVSMPAAADAAIREGLAAGESFEGILARAGERMRQQVNRLVDQYSKLVL
                     PPAPYYSVLMDGCLERGQDLSRSQKYNNFGIHGACSANAA"
     gene            complement(1466..2281)
                     /locus_tag="VIL39_09545"
     CDS             complement(1466..2281)
                     /locus_tag="VIL39_09545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5481710.1"
                     /translation="MSSSRTGRSGCANDRLNASTPMLTGVVFDIKEFAVHDGPGIRVT
                     VFLKGCPLRCSWCHNPEGLSPQPQVLRSAAEERVAGQTFTSTELAARLNSQAELLRGE
                     GGVTFSGGEPLAQAAFVAEVIDQLDDLHVVLDTSGYAEEPDFFAVASRADLVYFDVKL
                     VDPEQHRQHTGASNERIHRNLRVLSGLGVPFVIRVPLIPGVTDTEANLSGIARLVAGM
                     PGLRRVDLLPYNRAAGAKYAACGLKFQPTYDESRPVNPDTACFASAGLEVSVL"
     gene            complement(2241..3251)
                     /locus_tag="VIL39_09550"
     CDS             complement(2241..3251)
                     /locus_tag="VIL39_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5481711.1"
                     /translation="MKTLHLIQNLALSALAVWLFAGCGNQAAQTASPATGKKPKSEYV
                     FALVAKSQSNPVFQAARVGAEDAAVELSRKLGVKITVLWRTPNEDDAQKQVEYLEQLV
                     LQGVDGISVSCSEAETLTPAIDGAVKQGVAIMCFDSDAPASQRFCYHGTDDVESGHTI
                     MRELAAVLGPGKHVVAIPGGNQNARNLQNRIKGALDEAKLHPDLTIRGVYYSKEVPQD
                     AAAKIEEVQTANPDIDGWAMIGSWSLFTDALLKWEPGKVKIVAMDALPAELPYVRKGV
                     VQKLFAQQTYQWGWRSIELLADKVVLGKAPAKVLDYSPLPAVTKANVDEFEQNWKKWL
                     RQ"
     gene            complement(3275..4396)
                     /locus_tag="VIL39_09555"
     CDS             complement(3275..4396)
                     /locus_tag="VIL39_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5481712.1"
                     /translation="MPVPSPRRRAHFKETGLLGVVLALGLMLSVFGGSLQVRDRDSGD
                     THTVNKFLRADNLDKLAKNTSFFAIMAIGATFVIITGGIDLSVGSIYCLAAVCGAMVL
                     NYFGPRGAGAGASAVWVVPMAMVVCLGVGTLCGLLNGVGVVWLRVHPFVITLGTMAIY
                     RGVAFVMTKAQAFTHFPAAFTDGFIRYKVVWGSANLYPVPLAIMIGVTLLAGWFLRRA
                     VTGRHIFAVGGNEQASLFSGLPVSGVKLKVYAFSGLSAGIAAMIMLGYYGSASSDAGK
                     GYELDVIAAAVVGGASLAGGRGTALGALLGALIIQMINNGIVILNIDQNYSQIIIGAV
                     IVLAVLLDRVNSVLREKRLSRAAQLVKESRREEVCEPKP"
     gene            complement(4441..>5360)
                     /locus_tag="VIL39_09560"
     CDS             complement(4441..>5360)
                     /locus_tag="VIL39_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5481713.1"
                     /translation="DGLMRMMIGRSLAKYFPAHAACAPGPERLRVEGLSSAGKFRDVS
                     FRLCAGEVLGVAGLVGSGRSEVALGIFGLDPAVTGSVFLDQRPVLIRNPAEAMALGLG
                     LVGEDRKRQGLVPEMSCGENVTLASLHCPGGRGVWGRLVHCPVCPRRTECLRIWDFIR
                     LGSERQWVAKFFRRLGVRAASPEVPAVTLSGGNQQKLILARCLARNCRVLILDEPTRG
                     VDVGAKAEIHRLIDELASAGHAILMISSELPEVLNLSTRVLVMRNGRVAGMLERSDAT
                     PEKVIQLMAGRSDASHTPPQTAFKALSCN"
BASE COUNT         1020 a         1788 c         1580 g          972 t
ORIGIN      
        1 gcggcattgg ccgaacaggc gccatgaatg ccaaagttgt tatatttctg tgagcgcgaa
       61 agatcctgtc cgcgctccag gcaaccatcc atcaatacgc tgtaataggg tgcgggtggc
      121 aggaccagct tggaatactg atccaccagc cggttgacct gctggcgcat ccgctccccg
      181 gcgcgggcca ggattccctc gaagctttca cccgcggcca gcccctcgcg gatggccgcg
      241 tcggccgccg ccggcatgga aaccgcgccg atgtttacga cctccattcc ccggccgggg
      301 atgaggaact cccagcaggc cgccacggaa taatcccggg cgtcctcaag ctcgtagcca
      361 tgcgccacca ggccgggaat aaccacgtcg tcgttcgagt attgcggaaa ccccagccca
      421 atttccgtta attctgccgc cagttccagc aggtcaaggc ttgtgccctt cgtcacgcgc
      481 agattgatct tgggatcaat catcgccacg ccccgcgcca ccctcagcac caggtaagtc
      541 aattcgttca ccccgtcggt gccatcccgt ttgaccccgc cgagcatcag cgattgcccg
      601 ttgtcgccct gctggatgcc gggatataaa tcactgtccc ggttcagtga caggaagaat
      661 tccgccagca gccgttccgc gccggccagg tccaggcggc ctgccgccag gtccgcctgc
      721 aaatatcgcc acatgtattg atcaaaccgt ccgagtccgc agtggtaatg cccgctaatc
      781 caaagcaccg agtggcaaag gcggagggac tgaagggctt cgtgaaacgt acgcggggtg
      841 taggctggga cccgctccaa ggtctgcgca atatcaagcc ggcctcggcg atgcgcctca
      901 gccgcgtaac gtcgggccag gttgagcacc gcgtcaatgg tctccacggc gcaatccagg
      961 aattcaaccg cttcggcgtc gtgcttcatc cgcgcgcgcg tggccagcgc cacctgccgg
     1021 cgtcccgcca atccctctcg cagcaccatc ccccagtccg cgcaaatgtt gctgatcggc
     1081 cccagctcgt gcagcttgcg acccaacgtc agcgcactca tttgggcgtc gctgtatatg
     1141 cgcgggaact tcgtcgcggt ttgcgtgaat acgatctgct cctcgggacc aatgacaacc
     1201 tgctgcgcct cgcacatgcg ccgggtcaag cgggccgctc gttgcggcca cgaaagctgc
     1261 tccttctcac attcggccaa catatccact tccggcgacg tgcgaaaccg ctggtagctg
     1321 ccgtcgcgga gcgactgctt catctgcgcc aggcgcggct ccagttggaa gccctcgcgc
     1381 cttcttgtgc cgccggggcc ggccttgcct gcggcggaaa ctgcctgggc agatttcggc
     1441 caactcgtga ctgggctgct ggtcgtcata gaacactcac ctcgagaccc gcggacgcga
     1501 aacaggccgt atcgggattc accggccgtg attcgtcgta tgtgggttgg aatttcaagc
     1561 cgcaggcggc gtacttcgcg ccggcggcgc ggttgtaagg cagcaaatct acacgccgca
     1621 ggcccggcat gccggccacc aggcgtgcga tgccggacag gttcgcctcg gtatccgtca
     1681 ccccgggaat caacggcacc cggatgacaa agggcacgcc gaggccgctc agcacgcgga
     1741 gattgcggtg aatgcgttcg ttgctggcgc cggtgtgctg gcggtgctgt tccgggtcaa
     1801 ccagttttac atcgaaatag acgaggtcgg cccggctcgc caccgcgaaa aagtcaggtt
     1861 cctccgcata gccggaagta tcgagcacga cgtgaagatc gtccaactgg tctatcactt
     1921 cggcgacgaa ggccgcttgg gccagcggtt ctccgccgga aaacgtcacc ccgccttcac
     1981 cccggagcaa ctccgcctgg ctattgaggc gcgcggcgag ttcggtcgaa gtgaaggtct
     2041 gcccagccac gcgctcttcg gctgcactcc gcaggacttg cggttgcggc gacaagcctt
     2101 caggattgtg acaccaggaa cagcgcaatg ggcagccttt caggaatacg gtaacccgga
     2161 tgcctggccc gtcatggacc gcgaactcct tgatatcaaa tacgacgccg gtaagcatgg
     2221 gtgtggaggc attgagccgg tcattggcgc agccacttct tccagttctg ctcgaactca
     2281 tccacgttcg ctttcgtcac ggccggcagc ggcgagtaat ccagcacctt ggctggggcc
     2341 ttcccaagca caaccttgtc ggcgagcaac tcgatcgaac gccagcccca ctggtaggtt
     2401 tgctgcgcga atagtttctg caccacccct ttccgcacgt agggaagctc agcgggaagc
     2461 gcgtccattg ccacgatctt caccttgccc ggctcccatt taagcagcgc atcggtgaac
     2521 agactccacg aaccgatcat cgcccagccg tcaatgtccg ggttcgccgt ttggacttcc
     2581 tcaatcttcg ccgctgcgtc ttgcggcact tccttgctgt aatagacacc gcgaatcgtg
     2641 aggtcgggat gcagcttcgc ttcgtccagc gcgcccttga tccggttctg caggttgcgg
     2701 gcgttctggt tgccgccggg gatggcgacg acatgtttgc ccggaccgag cactgcggcg
     2761 agttcccgca tgatcgtgtg gccgctttcg acgtcatccg tgccgtgata gcagaagcgc
     2821 tggctggcgg gggcgtcgct gtcgaagcac ataatggcga cgccttgctt cacggccccg
     2881 tcaatcgccg gcgtgagcgt ctccgcctca gagcagctca cgctgatgcc gtccacgcct
     2941 tgcaggacca attgttcgag atactcgacc tgcttctgcg cgtcatcttc gttcggcgtg
     3001 cgccaaagca cggtgatctt cacgcccagc ttccggctca gttccaccgc ggcgtcctcg
     3061 gcaccgacgc gcgcggcctg gaagactggg ttggattggc tcttagctac cagcgcgaac
     3121 acgtattcgg acttgggttt cttccccgtt gcgggcgaag cggtttgcgc cgcttggttt
     3181 ccgcagccgg caaacagcca gacggccagg gcgctcaggg cgaggttttg aatcagatgc
     3241 agagttttca taaatgttcg gactgggatt ggtatcaggg ttttggctcg cagacctctt
     3301 cgcggcgtga ttccttcacg agctgtgcag cgcgggagag gcgcttttcg cgaaggacgc
     3361 tgttcacgcg atccagcagc accgccagta cgataacggc gccgatgatg atctggctgt
     3421 agttctggtc gatgttcagg atcacgatgc cgttgttgat catctggatg atcaaggcgc
     3481 ccagcaacgc gcctagcgcc gtgccacgtc ctcccgccag actcgccccg cccaccaccg
     3541 ccgccgcgat gacgtcgagt tcgtagccct tgcccgcatc cgacgacgcc gagccatagt
     3601 agcccagcat gatcatcgcg gcgatgccag cgctcagacc gctgaaggca tagaccttga
     3661 gcttcacgcc gcttactggc aggccgctga acagcgacgc ctgctcgttg ccgccgacag
     3721 cgaagatgtg ccgcccggtt accgcgcgcc gtagaaacca gcccgccagc aaggtcaccc
     3781 cgatcataat ggccagcggt acgggataga gattggcact gccccaaacg actttgtagc
     3841 ggatgaagcc atcggtgaac gcggcgggga aatgcgtaaa ggcttgagcc ttggtcatca
     3901 caaacgccac gccgcggtag atcgccatcg tgcccaacgt gatcacgaag ggatgcaccc
     3961 gcaaccaaac gacccccaca ccgttgagca acccgcacaa ggtgcccaca cccagacaaa
     4021 ccaccatcgc catcggcacg acccaaaccg cggaagcgcc cgccccggct ccccgcgggc
     4081 caaagtagtt cagaaccatc gcgccgcaga cggccgccag gcagtagatc gagcccaccg
     4141 acaggtcgat cccgccggtg atgataacga acgtggcgcc aatggccatg atggcgaaga
     4201 aggacgtgtt cttggcgagc ttgtccagat tgtcggcacg cagaaactta ttcacggtgt
     4261 gcgtgtcgcc gctgtcgcgg tcacggacct gcagtgaacc gccaaagacc gagagcatta
     4321 gccccaacgc gagcactacg ccgagcagcc cggtttcctt gaaatgtgca cggcggcgcg
     4381 gcgatggcac aggcacctcg gttggcatta ccgcgggtag agtttgggaa tcggtttcca
     4441 ttagttgcag gacagcgcct tgaacgcggt ttgcggtggt gtatggctcg cgtcgcttcg
     4501 tccggccata agttggatca ccttctccgg cgtcgcgtca gaacgttcca acattcccgc
     4561 cacgcggcca ttccgcatga ccagcacccg ggtgctcagg ttcagcacct ccggcagctc
     4621 ggaagagatc atcaggatgg cgtgaccggc tgaagcgagc tcatcaatga gccgatgaat
     4681 ctccgccttc gcccccacgt ccacgccgcg cgtcggctcg tcgagtatca gcacgcggca
     4741 gtttcgcgcg aggcagcggg cgaggatgag cttttgctgg ttgccgcccg acagtgtgac
     4801 cgccggcacc tcgggcgaag ccgcgcgaac acccagccgc cggaaaaact tggctaccca
     4861 ttgccgctcg ctgccaagcc gaatgaaatc ccagatccgc aggcactcgg tccggcgcgg
     4921 gcaaaccggg caatgaacca accgccccca gacaccgcgc ccgccgggac aatgcagcga
     4981 tgccaaggtg acgttttcgc cgcaactcat ttccggcacc aggccctgac gcttgcgatc
     5041 ctcgcctacg agacccagcc ccagcgccat ggcttcggcc gggttgcgga tgagaacggg
     5101 ccgctgatcg aggaacacgc tgccagtcac tgcggggtcc agtccgaata tcccgagcgc
     5161 aacttcgctg cggcccgaac cgaccaatcc cgctaccccc aacacctcac ccgcacaaag
     5221 ccggaagctc acgtcgcgga acttcccggc agatgagagc ccttcgaccc gcaagcgctc
     5281 cggcccggga gcacacgcgg catgagcggg aaaatacttc gccagcgaac ggccaatcat
     5341 catgcgcatc aggccgtcag
//
LOCUS       DATCGS010000465         3361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_486207, whole genome shotgun sequence.
ACCESSION   DATCGS010000465 DATCGS010000000
VERSION     DATCGS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486207"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VIL39_09565"
     CDS             complement(<1..440)
                     /locus_tag="VIL39_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481714.1"
                     /translation="MKIQRREFLKSTVALAAISGLPASLAVAAGSAGSYPYLGRTEDY
                     AEFRIIDPGLTINKVESWTQGQHGIVRVTTNDGHEGYGQLSTFEPDITATVLHRQVVR
                     HVLGSDPAQIDALVDRVIDANMKFPWSYVCRALAGVDTAIWDLYG"
     gene            complement(437..1069)
                     /locus_tag="VIL39_09570"
     CDS             complement(437..1069)
                     /locus_tag="VIL39_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5481715.1"
                     /translation="MKGKRKSKVRRWDVITLGNLSRNRYWGEGDDRGVRSAICTCTVI
                     QGEGFRLIVDPSLAKAEQMTAELDRRTGLKLRDIDAVFVTHEHADHWFGLAHFPEARW
                     LAAQDVAAALNKTHKLPKQVESATGKLFDAVEIIATPGHTLSHHSLRFDCDGVSVVVA
                     GDAVATLDFWRDRRGYFNCVDFDLSARSMNAIAGLADIVVPGHDNFFLSL"
     gene            complement(1290..2249)
                     /locus_tag="VIL39_09575"
     CDS             complement(1290..2249)
                     /locus_tag="VIL39_09575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5481716.1"
                     /translation="MNRRAFLKSVTAVCAVGTQASALVAADAPSPANAGSKLPRRRLG
                     KTGVEVSALALGGVIGMQLPPSPDHDPAALAEAALDLGITYFDTAPSYNNGQSETNYG
                     QVVARRRQEVFLATKTDNRTHDGTLRSIEQSLKRLRTDRVDLIQIHGISPNEDLDLWE
                     KPGGVLTALRKLREQKVTRFIGVTGHDSAGKLREAIERYELDTLLTTLNPVSRRQPFR
                     EDLLPVANRKQMGVIAMKVMGGGNGCLVTGNPLKKVLRPYHDETAHQADPAALIRYTL
                     SLPISVAVIGVASVEQLKANIRVVREATPMTLAERRHLETLMG"
     gene            complement(2286..>3361)
                     /locus_tag="VIL39_09580"
     CDS             complement(2286..>3361)
                     /locus_tag="VIL39_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481717.1"
                     /translation="EVWQREFEKRFGPEAAPFVEKALHQASWVLPRIVASCYPYSAFP
                     MTRGWAEKQRLGDLPAYAKAEGSDIQQFASFDEEAKMVIEGGETAKTRPGENSRWFAQ
                     TAADLNAQVAEAGQRIGSRRSKEFDSTITDLKILTNLSLFHSRRIHAAVSFRLFERTK
                     DPHALDDAIAAERSAVEAWRQLVAAAGDFYADDLMMGVRGASLCGHWKDELAALEAGV
                     AALERQRRELKPTNEARTAPQYRLVAPTGVGAAPAVVHQPVTTAPVGKPLTITAEVTA
                     PSGVKWVRLRYRSVNQHQDYRTLPMLPAGGIDHYRAVIPAEEIAPTWDLMYFIEAMDN
                     DRNGRMYPDLNKETPYIVVELKR"
BASE COUNT          572 a         1070 c         1063 g          656 t
ORIGIN      
        1 ccgtagagat cccagatcgc ggtgtccacg cccgccagtg cccggcagac gtacgaccac
       61 gggaacttca tgttggcgtc aatcacgcgg tccaccaggg catcaatctg cgccggatcg
      121 ctgccgagca catgccggac cacctgccgg tgcagcacgg tcgcggtgat gtcgggctca
      181 aacgtcgaga gctgcccgta gccttcatgt ccgtcgttgg tcgtgacccg aacaatgccg
      241 tgttggcctt gagtccacga ctcgaccttg ttgatggtca accccggatc aatgattcgg
      301 aactcggcgt agtcctcggt gcggcccagg tagggataac ttccggcgct cccggccgcc
      361 actgccaggc tagccggcag gccggaaata gcagccaggg cgactgtgct ctttagaaac
      421 tcgcgtcttt ggattttcat aggcttaaga agaagttgtc gtggccgggc accacgatgt
      481 cggccaagcc ggcaatcgca ttcatgctcc tggccgagag atcgaaatcc acgcagttga
      541 agtaaccccg gcgatctcgc cagaaatcca gggtggcaac ggcgtctccg gccacaacta
      601 cggacacgcc gtcgcaatca aaccgcaggc tgtggtggct cagcgtgtgc ccgggcgtcg
      661 caatgatctc gacggcgtcg aacaacttgc ccgtcgcaga ctcgacctgt ttcggcagct
      721 tgtgggtctt attcaaggcc gccgcgacgt cctgagccgc gagccacctg gcttcgggaa
      781 agtgcgccag gccgaaccaa tgatcggcgt gctcgtgcgt gacaaagact gcgtcaatgt
      841 cgcgcaactt cagcccggtg cgccggtcaa gttcggcagt catctgctca gctttggcca
      901 gcgagggatc cacgatcaag cgaaagcctt cgccctggat caccgtgcag gtgcaaatcg
      961 cggagcgaac gccccggtca tcgccctcgc cccagtagcg attgcgcgag aggttgccga
     1021 gggtaatgac gtcccaccgc ctgaccttgg atttccgttt gcctttcaat gaggcggatg
     1081 gcaaggcgcc tgcagccggc tcggcacccg agaccgctcc aagagctgta accgttccga
     1141 ggatgccgaa tgcggcgcgt gccagaaacg cagcgcgatt gtgtcggctg gcactgccac
     1201 tcgctttcag ctggggcttg cagggtggat ggggccggct catcgggttg caggttctcc
     1261 tcggcagggg atcgtgggcc acgctgtgct caacccatca aggtttcgag atggcggcgt
     1321 tcggcgagcg tcatgggcgt ggcttcgcga acgacgcgga tgtttgcctt cagttgctcc
     1381 accgacgcca cgccgatcac cgccaccgag ataggcaggc tgagcgtgta gcggatcagc
     1441 gctgcgggat cggcctggtg ggccgtttca tcgtgatagg gccgcagcac tttcttcaac
     1501 ggattgccgg tcaccaggca gccgttgccg ccgcccatga ccttcatcgc gatcacaccc
     1561 atctgcttgc ggttcgccac gggcaacagg tcctcccgga acggctgccg gcgcgacacg
     1621 ggattcagcg tggtgagcag cgtgtccaac tcgtagcgct cgatggcctc gcgcagtttg
     1681 cccgcgctgt cgtggccggt caccccgatg aagcgcgtga ctttctgctc gcgaagcttc
     1741 cgcagcgccg tgagaacgcc gcctggcttc tcccacaggt cgagatcctc gttgggggag
     1801 atgccgtgaa tctggatgag gtccacgcgg tcggtgcgca ggcgcttcaa gctctgctcg
     1861 atgctgcgca atgtgccgtc atgcgtccgg ttatcagtct tggtggcgag aaagacctcc
     1921 tggcgacggc gcgccaccac ctggccgtag ttggtttccg actggccatt gttgtagctc
     1981 ggcgcggtgt cgaagtaggt gatgcctaga tcgagcgccg cttccgccag cgccgccgga
     2041 tcatgatcgg gcgagggcgg aagttgcatg ccgatgacgc cgcctaacgc cagggcgctc
     2101 acctccacgc ccgtcttccc cagccgccgc cggggaagtt tcgagccggc gttggcaggc
     2161 gacggtgcgt cggccgctac gagggcggac gcttgagtgc caaccgcgca aacggcggtc
     2221 accgatttca ggaatgcacg acgattcata ggacttgcct ggtgttgact gggttcagag
     2281 tcgagttagc gctttagttc taccacgata tatggcgttt ctttgttcag atcgggatac
     2341 attctgccgt tcctgtcatt gtccatcgct tcgatgaaat acatcaggtc ccacgtcggg
     2401 gcaatctctt ccgccggaat caccgcgcgg taatggtcga tcccgcccgc cgggagcatg
     2461 ggcagcgtgc gatagtcctg gtgctggttg acgctgcggt agcgcaggcg gacccatttg
     2521 acgccggaag gggcagtcac ttcggcggtg atcgtcagcg gtttgccgac cggagccgtc
     2581 gttacaggct gatgaactac cgcaggcgca gcaccgacgc cggtcggtgc gactagccgg
     2641 tattgcggcg ccgtcctggc ttcattggtt ggcttgagct cgcgccgctg ccgctccaat
     2701 gcggcgacac ctgcttccaa ggccgccaac tcatccttcc agtggccgca caagctcgcc
     2761 ccccgaacac ccatcatgag gtcgtcggca taaaaatcgc ccgctgccgc cacaagctgc
     2821 cgccaggcct ccacagcgct ccgctccgcc gcgatggcgt cgtcaagtgc atgaggatct
     2881 ttggtccgtt caaacagccg gaagctgacg gcggcgtgga tgcgacgcga gtgaaagagc
     2941 gacaggttcg tcaggatctt caggtcggtg atcgtggagt cgaattcctt gctccggcgg
     3001 ctgccgatgc gctgcccggc ttcggcgact tgcgcgttga ggtccgccgc tgtttgcgcg
     3061 aaccaacggc tgttctcacc gggccgcgtc ttcgccgttt cgccgccctc gatgaccatc
     3121 tttgcttcct cgtcgaagct ggcaaactgc tgaatgtcgc tgccctcggc cttggcgtag
     3181 gcgggcaggt cgccgagccg ctgcttctcc gcccagccgc gcgtcatcgg gaacgcgctg
     3241 taggggtaac aggaggcaac aattcgcggc aggacccagc tcgcttggtg cagagctttc
     3301 tcgacgaaag gcgcggcttc cgggccgaag cgcttctcaa actcccgctg ccagacctcc
     3361 g
//
LOCUS       DATCGS010000466         8557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_633652, whole genome shotgun sequence.
ACCESSION   DATCGS010000466 DATCGS010000000
VERSION     DATCGS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633652"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VIL39_09585"
     CDS             <1..437
                     /locus_tag="VIL39_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481718.1"
                     /translation="TFDIDLIIRRTDQANWVDLARGAGYSFYREGPTFLQFNPPNAQA
                     LPLDLMLVGDDTFAKLMAEAVPGPASAAGAKVVSLRHLLALKCHAIKHGHEGRIVKDA
                     EDVIRLAQINKLDLDEPDIRGLFLKHGTAELYEKVRRLCRQG"
     gene            439..609
                     /locus_tag="VIL39_09590"
     CDS             439..609
                     /locus_tag="VIL39_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481719.1"
                     /translation="MAELEFPDWSGMDDSSARVTQDAAFQLCEQYRSWYPESAAKWRR
                     QEPEKCLVEFVL"
     gene            722..2116
                     /locus_tag="VIL39_09595"
     CDS             722..2116
                     /locus_tag="VIL39_09595"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY5481720.1"
                     /translation="MTYPEAIQFLYDLRWFGAKFGLANTFKLAALAGNPQDQLRFIHV
                     AGTNGKGSTCAMLESIYRTAGLRVGLFTSPHLVAFGERIQVNRRVISERDIGRLVAEM
                     RVLLAEGWGTPACDAHGSSTAPEAEARGSAEARSAAEHPTFFEVVTIMAARYFAAQEC
                     DLVIWETGMGGRLDATNIVTPLASVITNIQYDHQKWLGETLASIAAEKAGIIKPGIPV
                     ITAAEGEGVLQVITETARRQKAPLTTLSPAEAHRPPLDTLQLPLLGQHQRMNAAVAVA
                     TVRALASQIPVSDDKIRAGLSRVHWAGRLQLVTRPTGQKILLDGAHNISGAGILAAAA
                     QEFFPSAKFTLVLGILRDKDWAGMCEILAPLAGRILLVPVPSERSATPRELAEACRRA
                     NPRAQVFVYSSLAAALADAAKIPFIAIAGSLYLIGEAMELLHLSPGKANSERGLNEWG
                     STSCAGQSTATAKL"
     gene            2402..3412
                     /locus_tag="VIL39_09600"
     CDS             2402..3412
                     /locus_tag="VIL39_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY5481721.1"
                     /translation="MKIVAYSGYTSLLLALALAPFQSQAADKKVPTISVAKTEASSVA
                     GWQPAMGEGLAQMIITEMVNLSNFKVLESVALDDLRAERALGESGEVAQSESVKKGQW
                     KGADYTFKSTVTRFGSKEQNFGGGGIPVPTPFGIGGGHFGVRKNENEVQIDWRIIDNA
                     TREIVQGASGRAVGVEKGTGFNFGTWGGGGFSNNREFMDSALGKATMKALAQIIDKVK
                     TLNVGPGASTLNNEAAAAQNAAAVRNVKGVVKLVDGAEIWVSLGANSGFAKGDKIKIY
                     QPIEKKNKKGEVVATTYEPVAEIILTKVQKDKSMGTYTGTAKIAEDYVAADAAVDIEK
                     LE"
     gene            3597..4319
                     /locus_tag="VIL39_09605"
     CDS             3597..4319
                     /locus_tag="VIL39_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEY5481722.1"
                     /translation="MNIITRVFRSSLGKKYIMAVTGFGLFLFVIGHLAGNLQIFLGPE
                     AINRYGHFLQSNPELIWPVRLTLLLMVGLHIWSAVMVSLENKAARPVGYAVYQPVGSS
                     YASRAMLGGGSVIFVFIVYHLLHYTAEVQYLNLSGQDFAAFMEKLPGQVPAERHDIFK
                     MMVVGFSKPLVSGFYVLGLALLCLHLSHGASSMFQSLGWKSDAYRPFLDQAARVVAVL
                     IFLGYTSIPLSILCGYGKEALK"
     gene            4316..6232
                     /locus_tag="VIL39_09610"
     CDS             4316..6232
                     /locus_tag="VIL39_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY5481723.1"
                     /translation="MNLDAKIPTGPLAQKWDKHKFNLKLINPANKRKFEVIVVGSGLA
                     GASAAASLAELGYQVKCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDFRAREANVYRLAQVSANIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALCRQISLGAVQMYPRTEMLDLVVVDGHAKGIVVRNLTTGGISSHA
                     GDAVVLATGGYGNTFFLSTNAKSCNVTSIWRAYRKGAFFANPCYTQIHPTCIPVSGEH
                     QSKLTLMSESLRNDGRVWVPKRPEDCGKPPGSIPEADRDYYLERKYPSYGNLTPRDIS
                     SRAAKKACDAGRGVGPGGRGVYLDFADGIKRLGGNVIRERYGNLFDMYEKITGENAYR
                     VPMRIYPAVHYTMGGTWVDYNLMSTLPGLFVLGEANFSDHGANRLGASALVQGLGDGY
                     FILPYTIGNYFVTSKQPKPSASHPEFKKAEADVKAFTSRLLSIKGKRTPTSLHRELGK
                     LLWEKCGMARNEAGLKEALTRIPELREEFWKNVNVTGENAELNQSLEYAGRVADFMEF
                     AELLCLDALHRRESCGGHFREEFQTPDGEAQRDDQNFSYVAAWEYQGTDKPPVLHKEP
                     LVYEEVQMSTRSYK"
     gene            6229..6978
                     /locus_tag="VIL39_09615"
     CDS             6229..6978
                     /locus_tag="VIL39_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY5481724.1"
                     /translation="MNLTLKVWRQKNSNTPGRFETYQARNVIPDMSFLEMLDVVNEEI
                     IGRGEEPIAFDSDCREGICGMCSLVISGIPHGGHKGTTSCQLHLRHFKDGDTVTIEPW
                     RAKAFPIIKDLVTNRSAFDRIIQAGGFVSVNTGGTPDGNAIPVPKTAADRAMDAAACI
                     GCGACVAVCKNASAMLFVAAKVSHLNLLPQGKAEKDRRVLNMVRQMDKEGFGNCTVTG
                     SCEAVCPKEISLSFIAKMNRDYGVAILKGRH"
     gene            complement(7182..7913)
                     /locus_tag="VIL39_09620"
     CDS             complement(7182..7913)
                     /locus_tag="VIL39_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5481725.1"
                     /translation="MKIHCITRRCWPLLLLVTAASGFSAAPVNQLSAEEKADGWKLLF
                     DGKTTEGWHTFKTQSFPDKGWVVEDGWLHCLGKGGGNKGGGEIISDGQFNDFELQWEW
                     KLASAGNSGVKYFILESRKDALGHEYQMIDDEREPDAVKGGGKHLTASFYDVLAPTTR
                     PPPVKPSGEINSSRLIIKGNHVEHWLNGVKVLEYECGSEAVKAAVAASKFKTTVGFGD
                     KVRGHLLLQDHNSEVWFRNVRIREL"
     gene            complement(7928..>8557)
                     /locus_tag="VIL39_09625"
     CDS             complement(7928..>8557)
                     /locus_tag="VIL39_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481726.1"
                     /translation="GLLPPDWNWSDPQRLNEAMKTLTDALRPWTIDFHVAQNDATVHG
                     SGTHDKTGHHCLPNDPNGKLTIAHHAGYWLHGEDGRLTRAIRHICWDGCMFPNAVMEK
                     QETWNDILRAMIAVRDAHGWREEAKPAAKAETPSVAVKTRTGAKPARKKAVRVVPRKP
                     VKKAVKKVTTKAKFKPLRGKAKLRKAKAVKKLNVKAKKSGSKKTARRKK"
BASE COUNT         1718 a         2609 c         2560 g         1670 t
ORIGIN      
        1 acacgttcga catcgatctt atcatccgcc gaactgatca ggctaactgg gtggacctgg
       61 cgcgcggcgc gggctattcc ttttatcgtg aaggaccgac gtttctccaa ttcaacccgc
      121 cgaacgctca ggccctgccg ctcgatttaa tgctggtcgg cgatgacacc tttgcgaagc
      181 ttatggcgga agccgtgccc ggccctgcca gtgcggcggg ggctaaagtt gtctcgctgc
      241 ggcacctgct cgcgctcaaa tgccatgcaa tcaaacacgg ccacgagggc cggattgtta
      301 aggacgccga agatgtgata cgtttagcgc agatcaacaa gcttgatttg gatgagcccg
      361 atattcgcgg cctctttctg aaacacggca cggcggaact gtatgaaaaa gtgcgacgac
      421 tctgcaggca aggctgaagt ggcggaactc gagttccctg actggagcgg catggatgat
      481 tcctctgcca gggtcaccca ggatgccgcg tttcaacttt gtgagcagta ccgctcgtgg
      541 tatccggaat cagcggctaa atggcggcgg caagagccgg agaagtgcct tgtcgagttt
      601 gtgctctaaa cttgctgccc gcgctcaagg cacgggtgtt ttcggacgtg acgttgcgaa
      661 agtcaggagt tagacgtcgg ccccgagccc aacacacacc caaatcgata ttccttaact
      721 aatgacctac cccgaagcca tccagttcct ttacgacctt cgctggttcg gcgcgaaatt
      781 cggcctggct aacacgttca agctggccgc cttggctggc aatccgcagg accagctccg
      841 cttcatccac gtcgccggta cgaatggcaa aggctccacc tgcgcgatgc tggagagcat
      901 ctatcggacg gcaggcttgc gggtcggctt gttcacctcg ccgcacctgg tcgcctttgg
      961 cgagcgcatt caggtcaatc gtcgcgttat ctccgagcgc gatattgggc gcctcgtcgc
     1021 ggagatgcgg gtgttgctgg cggaagggtg ggggaccccc gcctgcgacg cccatggttc
     1081 cagcacagcg cctgaagctg aagcacgcgg ctccgcagag gctcgttccg ctgccgagca
     1141 ccccaccttc tttgaggtcg tgaccatcat ggcggcgcgt tacttcgccg cgcaggagtg
     1201 tgatctggtg atatgggaaa ccgggatggg tggccgactg gatgccacga acatcgttac
     1261 acccctggcc agcgtcatca ccaacatcca atacgatcat cagaagtggc tgggtgaaac
     1321 cctggccagc atcgcggctg agaaggcggg cattatcaaa ccgggcattc ccgtcattac
     1381 cgcggcggaa ggagaaggag tgctccaggt gatcacggaa acggcccgcc ggcaaaaggc
     1441 accgctgaca accctctcac ccgcggaagc gcatcgtccg ccactggaca cccttcaact
     1501 gccgctgctc gggcagcatc aaaggatgaa tgccgcggtg gccgtcgcca ccgtgcgcgc
     1561 tctcgcttcg cagattccgg tgagcgacga caagatccgc gccggcctct cccgcgtcca
     1621 ctgggccggg cggctccaat tggtcacacg gccaacgggc cagaagatcc tgctcgacgg
     1681 cgcccataat attagtggcg ccggaattct ggcggcggcg gcacaggagt tcttcccctc
     1741 tgcgaaattc accctggtgc tgggcatcct gcgcgacaag gattgggccg gcatgtgcga
     1801 aatcctcgcg ccgctggcgg ggcggatcct gctggtgccg gtcccaagcg agcgcagcgc
     1861 cacgccacgg gagcttgcgg aggcttgccg acgcgccaat ccgcgggcgc aggtcttcgt
     1921 gtactcctcg ctggcggcgg cgctcgcgga cgcggctaag atcccattta tcgccattgc
     1981 cggttccttg tatttgattg gtgaggcgat ggagttactc catctctcgc ccggcaaggc
     2041 aaacagcgag cgcggcctca acgaatgggg cagcacctct tgcgctggcc aatccacagc
     2101 cactgcgaaa ctatgaccct ccaggaacgg ccgcaggaag aaccgaaagc gggcacaaag
     2161 gccggtgggg aaaatccaac tgtgctttcg cggcggcagt tgcaacccgc ttggccaggt
     2221 cgaaaagagt tgtcggacga aaagagtttg ccaaatggat ttcattagcg cataggtatg
     2281 cgtgtatggg ttttgaaggg attagaaaat gagtaaatcc gtcgttgggg gctcctgccg
     2341 ggtcagggaa aactggttgt ccggcagcgg agtcttcgac tacaatagtc agcgtcacac
     2401 tatgaaaatc gtcgcatatt caggttacac ttctctgctc ttagcgctcg cgctcgctcc
     2461 gtttcaaagt caggcggccg acaaaaaggt gccgaccatc agcgtggcaa agaccgaagc
     2521 cagcagcgtg gctggttggc aaccggccat gggggaaggc ttggcccaga tgatcatcac
     2581 ggagatggtc aacctatcta atttcaaggt cctcgaatcc gtcgcgctcg atgaccttcg
     2641 cgccgagcgc gctttggggg aaagcggcga ggtggctcag agcgaaagcg tgaagaaagg
     2701 ccaatggaag ggggccgact ataccttcaa gagcacagtc acgcgctttg ggtccaaaga
     2761 acagaacttt ggcggcggcg ggatacccgt gccgactccg tttggtattg ggggtggtca
     2821 tttcggagtc cgcaagaatg aaaacgaggt gcagattgac tggcgaatca tcgataacgc
     2881 tacgcgggaa attgtgcaag gggccagcgg gcgggccgtc ggcgtcgaga aaggcaccgg
     2941 gttcaacttt ggcacctggg gcggaggcgg cttcagcaat aaccgagagt ttatggacag
     3001 cgcgttgggc aaggcgacca tgaaggcgct ggcccaaatc atcgataaag ttaagactct
     3061 caacgtcggc cctggcgcca gcacccttaa caacgaagcc gcggcagcac aaaacgccgc
     3121 cgctgtccga aatgtcaagg gagtggtgaa gttggtggac ggcgcggaaa tctgggtctc
     3181 gctgggcgcc aacagcggct ttgccaaggg cgacaaaatc aaaatctacc aaccgatcga
     3241 gaagaagaac aagaaaggcg aagtggtcgc cacgacctac gaacccgtgg ccgagatcat
     3301 cctgaccaag gtgcagaaag acaagagcat gggcacctac accggcacgg ccaagatcgc
     3361 cgaagattat gtggccgctg acgccgcagt ggacatcgag aagctcgaat agcagagctc
     3421 ggcaaccgga aatcagaggc ctgatccgcg ccaattgacc cggccattcg caactcggtc
     3481 ctgcgcccat ccgctgggct ggcgtagtgt gttttcttga cagaagccga ccaacaacta
     3541 ggttccatgt attgcgcagc catgggcaac ctccggctgg ccgggcgcga ttgccgatga
     3601 atattattac gcgtgttttc aggtcgtcac tcggtaagaa gtacatcatg gccgtaacgg
     3661 gcttcggcct cttcctgttt gtgatcggac acctggctgg caatctgcaa atctttcttg
     3721 gcccggaagc catcaatcgc tacggccact ttctgcaatc caaccccgag ttaatctggc
     3781 ccgtccggct gaccctgctg ctaatggtgg gcttacacat ctggtcggcg gtgatggtct
     3841 cacttgagaa taaggcagcg cggccagtgg gctatgcggt ttatcagcct gtcggctcca
     3901 gctacgcgtc gcgcgcgatg ttggggggcg ggagcgtgat ctttgtgttc atcgtctatc
     3961 atctgctgca ctacaccgcc gaggtgcaat accttaatct ttccgggcag gacttcgccg
     4021 ccttcatgga gaagctcccc ggccaggttc ccgctgagcg gcacgacatc ttcaagatga
     4081 tggttgtggg cttcagcaaa ccgttggtct cgggcttcta tgttctgggc ctggcgctgc
     4141 tatgcctgca cctcagccac ggggcgagca gcatgttcca atcgctgggc tggaagagcg
     4201 atgcctatcg gccgtttctc gaccaggcgg cacgggtggt cgcggtgcta atctttctcg
     4261 gctacacctc catcccgctc tcaatacttt gcggctatgg aaaggaggca ctgaagtgaa
     4321 cctggacgcc aagataccca ccggcccgct ggcccaaaag tgggacaagc acaagttcaa
     4381 cctcaagctg atcaacccgg ccaacaagcg caagtttgag gtcatcgtcg taggcagcgg
     4441 cctggcgggg gcgtccgccg ccgcctcgct ggccgagctg ggctaccagg tcaaatgctt
     4501 ctgcttccag gacagcccgc gacgcgccca cagcatcgcc gcccagggag ggataaacgc
     4561 cgccaagaat tatcagaatg acggggacag cgtttaccgg ctgttctacg acacaattaa
     4621 agggggcgac ttccgcgcgc gcgaggctaa cgtctatcgc ctcgcgcagg tcagcgcgaa
     4681 catcattgac caatgcgtgg cccagggcgt gcccttcgcg cgcgaatacg gcggcctgct
     4741 ggcgaaccgt tcgtttggcg gggcgcaggt ctcgcggacg ttttatgcgc gcggccagac
     4801 cggccagcag cttctgctcg gcgcttacca ggcgctttgc cgccagatca gcctcggcgc
     4861 ggtgcagatg tatccgcgga cggagatgct cgacctggtt gtggtggacg gtcatgccaa
     4921 aggcatcgtg gtgcgcaacc tgaccaccgg cggaatctcc tcccacgcgg gcgacgcggt
     4981 tgtgctggcg acgggcggat atgggaacac attcttcctc tccaccaatg ccaagagctg
     5041 caacgtcacc tccatttggc gtgcctaccg gaagggcgcg ttcttcgcga acccctgcta
     5101 cactcagatt catccgacct gcatccccgt cagcggcgag caccagtcca agctcacgct
     5161 catgtccgag tcgctgcgca acgatggccg cgtctgggtg cccaagcgcc cggaggattg
     5221 cggcaagccg cccggatcga ttcccgaggc ggaccgcgat tactatctcg aacgcaagta
     5281 tccgagctat ggcaacctga cgccccggga catctcctcg cgcgcggcca agaaagcctg
     5341 tgatgctggc cgcggggtcg gcccgggcgg tcggggcgtc tatctcgact ttgcggatgg
     5401 catcaagcgg ctgggcggga atgtcatccg ggaacggtat ggcaacctgt tcgacatgta
     5461 cgagaagatc accggcgaga atgcctaccg ggtgccgatg cgcatctacc cggcggtcca
     5521 ttacaccatg ggcggcacgt gggtggacta caacctcatg agcaccctgc ccggcttgtt
     5581 cgtgctcggc gaggccaact tctccgatca cggcgccaac cgcctcggcg ccagcgccct
     5641 ggtgcaaggg ctgggcgacg gctacttcat cctgccctac accattggca attactttgt
     5701 aacgtccaag caaccgaagc cctccgccag ccatcccgag ttcaagaaag ccgaggcgga
     5761 cgtcaaagcc ttcaccagcc gcttgctttc aatcaagggc aagcgaaccc ccacttccct
     5821 gcaccgcgag ttgggcaagc tgctttggga gaagtgcggc atggcgcgaa atgaagcggg
     5881 cttgaaggaa gcgctgacgc gcatcccgga gctgcgcgag gaattctgga agaacgtcaa
     5941 cgtcaccggc gagaatgccg agttgaacca gagcctggaa tacgccgggc gcgtggccga
     6001 cttcatggaa ttcgccgagc tgctctgcct cgatgcgctg catcgccgcg aatcgtgcgg
     6061 cggccatttc cgcgaggaat tccagacgcc ggacggcgag gcgcagcgcg acgaccagaa
     6121 cttctcctat gtggccgcct gggagtacca gggcacggac aaaccgccgg ttctgcacaa
     6181 agaacccctc gtctatgaag aagtccagat gagcacgcgc agctacaaat gaacctgact
     6241 ctcaaagttt ggcggcagaa gaactcgaac acccccgggc ggttcgagac ctaccaggcc
     6301 aggaacgtga tcccggacat gtcattcctg gagatgctgg acgtggtgaa cgaagaaatc
     6361 atcgggcgcg gcgaggagcc gattgccttt gactccgact gccgggaagg catctgcggc
     6421 atgtgctcgc tggtcatcag cggcatcccc cacggcggcc acaaaggcac cacttcctgc
     6481 caactgcacc tgcggcattt caaggatggc gacacggtca ccatcgaacc gtggcgggca
     6541 aaggcgttcc ccatcatcaa agacctcgtc accaaccgca gcgccttcga ccgcatcatc
     6601 caagccggcg gcttcgtttc ggtgaacact ggcggcacgc cggacggcaa cgccatccca
     6661 gttcccaaga ccgcggcgga ccgcgccatg gacgccgccg cttgcattgg ttgcggcgcc
     6721 tgcgtcgcgg tatgcaagaa tgcttccgcc atgctcttcg tcgccgccaa agtctcccac
     6781 cttaacctgc tcccgcaggg caaagccgag aaggaccgcc gtgtgctcaa catggtccgg
     6841 cagatggaca aggaaggctt cggcaactgc accgtcaccg gctcctgcga agcggtttgc
     6901 ccgaaggaaa tcagcctcag cttcatcgcg aagatgaacc gcgactatgg cgtggccatc
     6961 ctcaaggggc gtcactgact cagaatgaga ccatcgcgtt caatgccggc tcgcatatcg
     7021 aggtgatcaa gctggcctac aagggttttg agcggctggt acgaccaact gtgctaacct
     7081 ttaccacctg agcgaggtcc ttatcgtcaa gagcggctat cgcgccagcg cggtggagtt
     7141 gatgtcagac cctccgtggg cttgaggtcc ggcagcactc cctacagttc gcggattctc
     7201 acattccgga accagacctc actgttgtga tcctggagca agaggtggcc gcggactttg
     7261 tccccgaagc cgacggtggt cttgaacttg ctagccgcca ccgctgcctt gaccgcctcg
     7321 ctgccgcact cgtattccag caccttaacc ccgttgagcc agtgctcgac gtggttgccc
     7381 ttgatgataa gccgtgagga gttgatttcg cccgacggct tcacgggtgg tggccgggtc
     7441 gttggcgcga gcacatcata gaatgaagcg gtgaggtgct tgccgccgcc cttgacggca
     7501 tccggttcgc gctcatcgtc gatcatctgg tattcgtgcc ccagggcatc tttgcgcgac
     7561 tcgaggatga aatacttcac gccgctgttg cccgccgaag ccagcttcca ttcccactgc
     7621 aactcgaagt cgttgaactg gccgtccgag ataatctcgc cgccgccctt gttgccgccg
     7681 cctttgccca ggcaatgcag ccagccgtcc tcgacgaccc agcccttgtc gggaaatgac
     7741 tgcgtcttga acgtgtgcca gccctccgtg gtcttgccgt caaagagtaa tttccagcca
     7801 tcggcctttt cttcagccga gagctgattg accggagctg ccgagaaccc cgaagcggca
     7861 gtaaccaaca acaacagggg ccagcaccga cgcgtaatgc aatggatttt catagactta
     7921 ttgtaaccta tttcttccgc ctggctgtct tcttcgaccc agacttcttg gccttaacgt
     7981 ttagcttctt gacagctttt gctttgcgaa gcttggcttt gcctcggaga ggcttaaatt
     8041 ttgcctttgt ggttaccttc ttcacagcct tcttaaccgg tttcctgggc actaccctca
     8101 ctgctttctt gcgcgcaggt ttggcgcccg tcctcgtctt gactgcgacg gacggtgttt
     8161 ccgccttagc ggccggcttg gcctcttcac gccagccatg tgcatcgcgc accgcaatca
     8221 tcgcccgcag gatgtcgttc caggtttcct gcttctccat gaccgcgttg gggaacatgc
     8281 agccgtccca acagatgtgg cggatcgccc tcgtcagccg accgtcttca ccatgcagcc
     8341 agtaacccgc gtgatgggcg atagtgagct tgccgttcgg gtcgttcggc aggcaatgat
     8401 gccccgtctt gtcgtgcgtg ccggagccat ggaccgtcgc atcgttttgc gcgacgtgga
     8461 agtcgatggt ccacggacga agcgcgtcgg tgagagtctt catggcttcg ttcaagcgtt
     8521 gcgggtccga ccaattccaa tccggcggca ggaggcc
//
LOCUS       DATCGS010000467         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_89428, whole genome shotgun sequence.
ACCESSION   DATCGS010000467 DATCGS010000000
VERSION     DATCGS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89428"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VIL39_09630"
     CDS             complement(<1..289)
                     /locus_tag="VIL39_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY5481727.1"
                     /translation="MRNSLLLVFSVVFYAWGEVIFVFLMLGSALLNHFLGQWVDRETE
                     PSRRKWAVGVAVALNIGMLVFFKYANFAVANLNDLLAVVNMPPMHMDPVRLP"
     gene            complement(465..2360)
                     /gene="cysC"
                     /locus_tag="VIL39_09635"
     CDS             complement(465..2360)
                     /gene="cysC"
                     /locus_tag="VIL39_09635"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY5481728.1"
                     /translation="MSAPEPSPPPPTAAEQLKIVILGHVDHGKSTLVGRLLYDTGSLP
                     EGKLEQLQQASQRRGMPFEWANLMDALQAERDQNISIDTAQIWFRTPKRRYVIIDAPG
                     HKEFIKNMVTGAAQAEAALLLIDAQEGVQENSRRHGYLLELLGIRQIAVLVNKMDLVG
                     YSQERFSAIEAEYRAWLKQLGLEPSVVIPIAARQGDNVAAPSQNLPWWKGPTVLETLD
                     EFKAARAPGGQPLRFPIQDVYRFDHRRILAGRVESGRIKVGDRLVFSPSNKTSTVKTI
                     ERWNAPARDSAETGESIGITLTDQVFVERGVVASLEQTPPYALTCFKARVFWLGKRPF
                     APGRKYRLKLATQEVECQIEKLERVINSATLQTVERLESETFVGRHEAAELTLRTKKP
                     VAFDAINEVVATGRFVIVDGFDVAGGGVIVPDSYPRRTADSLHKSHNIFWTSGRITAG
                     QRALHQGHAGRVVWLTGLSCSGKTTIAAELERQLFARGKYSYVLDGDNMRHGLCSDLG
                     FSPKDRAENIRRVGEVAKLFADAGVICVTAFISPYRADRDTIRQNLPPGQFVEVYLNI
                     PVEACEQRDSKGLYAKARAGTIRDFTGVNAPYEPPTKPEIELPTAQLTVEECVSRIMA
                     FLAALEK"
     gene            complement(2368..>2849)
                     /locus_tag="VIL39_09640"
     CDS             complement(2368..>2849)
                     /locus_tag="VIL39_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY5481729.1"
                     /translation="KDALKTVLLQHNFTGVIVGVRRDEEPTRAKERYFSPRDKNMEWN
                     LEDQPPELWDQFKTDFEPGTHIRIHPLLHWTELNIWEYIERESVPVIPLYFANDKGER
                     YRSIGCHPCTFPIQSQARTVGEIVEELRRTRTPERAGRAQDKESESAFEKLRRDGYM"
BASE COUNT          512 a          916 c          872 g          549 t
ORIGIN      
        1 tgggcaggcg caccgggtcc atgtgcatcg gcggcatatt aaccaccgcc agcaagtcgt
       61 tcaggttggc gacggcgaaa ttcgcgtatt tgaagaagac cagcatgccg atgttcagcg
      121 ccaccgctac ccctaccgcc cacttccggc gggacggctc cgtttcccgg tccacccact
      181 gccccaggaa atgattcaac agcgccgagc cgagcatcag gaagacaaag atcacctcgc
      241 cccaggcata gaacaccacg ctgaaaacca gcaggaggct gtttcgcaac ctcagtcccg
      301 gcagggcggt aagggcgaga acaaccggca agaacaaaaa caaaaagatc ggcgaactaa
      361 aaaccatcgg tcaaacaagt gtgctggatt cttgccatgc aagacgccgg tgtcaatgcg
      421 gcgatggtca cgggcaaggt tcccgtcacg gcgagcgcca aattctattt ctccaaggcg
      481 gcgaggaacg ccatgatcct ggagacgcat tcctcgaccg tgagctgggc ggtgggcagt
      541 tcgatctcgg gtttcgtcgg cggctcatac ggcgcgttca cgccggtgaa gtcccgaatt
      601 gtgccagcgc gggctttggc atacagcccc ttggagtcgc gttgttcgca ggcctcgact
      661 ggaatgttca gatatacctc gacgaactga cccggcggca ggttctggcg gatcgtgtcg
      721 cggtcggcgc ggtaagggga gataaaggcc gtcacgcaaa tcactcccgc gtccgcaaac
      781 agcttggcca cctccccgac ccggcggata ttctcggcgc gatctttcgg ggagaaaccc
      841 aggtcggagc acaacccgtg gcgcatgttg tcgccatcca gcacgtaact gtacttcccc
      901 cgcgcgaaca gttggcgttc cagctcggcg gcgatcgtcg tcttgcccga acaggacagg
      961 ccggtcagcc acaccacgcg gcccgcgtgg ccctggtgca gcgcccgctg cccggcggtg
     1021 atccggcccg aggtccaaaa gatgttatgg cttttgtgca gcgaatccgc cgtgcgccgc
     1081 gggtaactgt cgggcacaat cacgccgccg ccggcgacat cgaacccatc cacaatgacg
     1141 aagcggccgg tcgccactac ctcgttgatg gcgtcgaagg cgaccggctt cttcgtgcgc
     1201 agcgtgagtt ccgcggcttc gtgccggccg acaaatgtct cggactccag acgctccacg
     1261 gtttggagcg tggccgagtt gatcacccgt tccagtttct cgatctggca ttcgacttcc
     1321 tgtgtcgcca gtttgagccg gtatttccgg cccggcgcga aaggccgttt gccgagccag
     1381 aagacccggg ccttgaagca ggtcaacgca tagggcggcg tctgctccag cgaggcgacg
     1441 acgccgcgct cgacgaacac ctggtcagtc agcgtgattc cgatgctttc gccggtctcg
     1501 gcggagtcac gcgccggagc attccagcgt tcgatggtct tcacggtgct ggtcttgttg
     1561 ctcggagaga agaccagccg gtcaccgacc ttgatgcggc cgctctcgac gcgcccggcc
     1621 aggatgcgcc ggtgatcgaa gcgatacacg tcctggatcg ggaatcgcag cggctggcct
     1681 ccgggagccc gggcggcttt gaactcgtca agcgtctcca acaccgtcgg acccttccac
     1741 cagggcaggt tctggctggg cgcggcgacg ttgtcgccct gccgggctgc gatggggata
     1801 acgacgctcg gttcgaggcc gagctgcttg agccacgcgc gatattcggc ctcgatggcg
     1861 ctgaaccgct cctgcgaata gcccaccagg tccatcttgt tgaccaggac cgcgatctgc
     1921 cggatgccca gcaattcgag caggtagccg tggcgccgcg agttttcctg caccccctcc
     1981 tgggcgtcga tcaacagcaa cgccgcctcg gcctgcgccg ccccagttac catgttcttg
     2041 atgaactcct tgtgccccgg cgcgtcaatg atcacgtaac gccgcttcgg cgtccggaac
     2101 cagatctgcg ccgtatcaat gctgatgttc tggtcgcgct cggcctgcag cgcgtccatc
     2161 aggttcgccc attcaaaggg catcccgcgc cgttgtgagg cctgctggag ttgctccagc
     2221 ttgccttcgg gcagcgagcc cgtgtcgtaa agcagccggc ccaccagcgt ggacttgccg
     2281 tgatccacat ggccaagaat gacgatctta agctgctcgg cggcagtcgg cgggggaggg
     2341 gatggttcgg gggcgctcat tggcaaatca catatatccg tcccgacgca gtttctcgaa
     2401 ggccgattca ctttccttgt cctgcgcccg gcccgcccgc tccggcgtcc gcgtgcggcg
     2461 cagttcctct acgatctctc ccacagtgcg ggcttgcgac tggatgggga acgtgcacgg
     2521 gtgacagcca atgctccggt accgctcccc cttgtcgttc gcgaaataga gtgggatgac
     2581 ggggacgctc tcgcgctcga tatactccca gatattcaac tccgtccagt gcaacagggg
     2641 gtgaatgcgg atatgtgtcc ctggctcaaa gtccgtcttg aattggtccc acagctccgg
     2701 cggttggtcc tcgaggttcc attccatatt cttgtcgcgc gggctgaaat agcgttcctt
     2761 ggccctcgtt ggttcctcgt cgcggcggac gccgacaatc acgccggtga agttgtgctg
     2821 cagcagcacg gtcttgagcg cgtccttct
//
LOCUS       DATCGS010000468         6575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_287007, whole genome shotgun sequence.
ACCESSION   DATCGS010000468 DATCGS010000000
VERSION     DATCGS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287007"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VIL39_09645"
     CDS             <1..887
                     /locus_tag="VIL39_09645"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY5481730.1"
                     /translation="LQGHGEHQLNGDGQTGYLKFASILQQNNIRVEPLSLLGTNTVPM
                     DCNLLVIAGPGQTLPDPELNKIEHYLSQGGRLLALFNCYPLYSGNKDTGLEGLLATWG
                     VDVGHNVIKDPVNTVSGENVRVSSFGKHILVNSLLLSELDLMLPRSVGQLKGHSQAAD
                     APRVEELAYSGSSSFALGDVAHKRSFPLMVAVEKGAIKDVITERGSTRMVVVGDSLFL
                     GNYEIDSGVNRDFAGNAVNWLLERTQLLEGLGPRPIEEHRIVMTRAQLHQAQWVLLAG
                     LPGSVLLLGGLVWLRRRK"
     gene            897..2690
                     /locus_tag="VIL39_09650"
     CDS             897..2690
                     /locus_tag="VIL39_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY5481731.1"
                     /translation="MNPKNTWLWIAVAAGLFVLVFFHQRHARNAGSGPIRVLPNLKAA
                     AVTSIQVRAGARTNIRAERTNGVWQLTKPLVYPAQAVSIESLLSQLERLTPAPYITAR
                     ELRNRPKVDEAYGFVAPQASIVIEQPDHPIHLSVGARTAHGDQVFLQVVGADGVYVVG
                     ADLLKYIPSTPEDWRDTTLIDLKGLAFDRLSVTNGAKIFELRRNATNKLWRMVYPLQA
                     RAYSARAEESLQLLQSVTIDRFISDDPKADLEAFGLQSPELEVAFGQGTNTAARLQFG
                     KRLTNDTRLIYARRIGLNGIVAVPKDLLAPWYALVNDFRDPLLVTLTTPVAVINVRGQ
                     DSFSVQEQTNGTWRIVPQGFPADAGLVKDLLSALSTMQIAEFRADNAIAQEWPRYGLA
                     SPTRQYALRSATTNSPAGPTNALIAELNFGTNEADKVFVRCADEPGFVYAVKLADFDR
                     LPSASWQMRERRVWNLSTNDVARATIRQQGRVCQIIRNGPYEWSLAPGITNDLAVEET
                     VSELCQLTAVAWVGRGAAARARYGFTDNSHQITLELKNGEKSSVEFSSGPSSSVPCAA
                     VTLDGEPWVFECPAWLYGYVQRYLSLPPNNP"
     gene            2693..5845
                     /locus_tag="VIL39_09655"
     CDS             2693..5845
                     /locus_tag="VIL39_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY5481732.1"
                     /translation="MATRQKGRFWRICRIYFRRVRITVWLLILALLGGLFYLNQIGLP
                     DFAKKPLLEKLRARGLDLQFSRLRLSWYHGLVAENVRFGPADEPLGPHLTLGEVELRL
                     NYPALARLQVQVDSLMLRKGSLVWPIAETNEAPRHLVVENIQTDLRFLPGDEWALDHF
                     TAGFAGAKIQLSGRVANASAIREWKFLGAERVAPANVPRSRLRQFADTLERTHFSAPP
                     ELRLDVQGDARDLASFGVRVMVIASGADTPWGTVTRGRFTARLFPATTNGLSNAELTL
                     EAGEAQTRWATMANLHLTAYLASFEGQTNLGNGNVTLHAGHVDTEWGSATNLQLTLHA
                     ASIEGQTNLVNADLALSAGAFETRWGSATNAQFHAQWIHSLTNPVPLTGEGRLDCSQA
                     TSEWGAAREIQLNARLATPATHTSPRADESWAWWAKLEPYVLDWDCHLRGLQSRGLEA
                     EDVACTGHWRAPDLTITNLHAELYQRELDVHADLDVATRELVLRLSSTLDPHKIAPLL
                     SEGAQRLLALFSWPEPPKLKGSLSLVLPAWTNRQPAWSAEVLPTLRLEGECQLEHGGA
                     YRDVAVSAVQAHILYSNRVWRLPDLKITRPEGRLEAMHEADARTGDYYFNINSTLDVR
                     ALRPLLEPGQQKGLDYLTFTEPPALEAEIRGNWHDVERIGIKGRVALTNFTFRGESVS
                     AVQTAFQYTNRFLQITSPRLQRGTQQLTADSLGADFLAQKIYVTNGYSTVEPMVVARA
                     IGPLIVRAIEAYQFRQPPVAHVYGTIPLHGEEDADLHFDLVGGPFDWWRFHVPKIQGH
                     VHWLGQQLTLSNVEVGFYGGQATGSAHFDFHPRDQTDYQFAVTATDALLQPFAADFFL
                     KTNRLDGRLSGTLVISNANTVDIQSWNGRGNLSLRDGLIWELPIFGIFSDAINGMAPG
                     LGSSRASAGTCTFGITNGIIRSDDVDIRSTGMRLQYRGTLDFQGQVNARVEAGLLRDM
                     WLVGPVVSTVLWPVTKVFEYKVTGSLGDPKAEPVYVPRLMFFPFQFPFHPIRTLKGLL
                     PEDLGSSRTNAPALSSPKQN"
     gene            5872..>6575
                     /locus_tag="VIL39_09660"
     CDS             5872..>6575
                     /locus_tag="VIL39_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5481733.1"
                     /translation="MSYTPPTPPPASDAWFVTTHWSVVLSAREKDSPRSAAALETLCR
                     TYWYPLYAYLRRQGRPPHDAQDLTQGFFARLLQKDYLQAAAREKGKFRTFLLVALKRF
                     LANEWDREHALKRGGFAQVVSIDQEVAESRFAAEPSHNVQPYVLFDRQWAMTLLERTM
                     TQLQEEYVASGRAKLFEYLQSCLAREESALPYAQIAACLNLTEPAVKMAVHRLRARYR
                     EILRAEIAHTVSSPEEV"
BASE COUNT         1221 a         2045 c         1964 g         1345 t
ORIGIN      
        1 atctccaggg ccacggcgag caccagctca acggcgacgg ccagaccggt tatctcaagt
       61 tcgcctccat cctccagcag aacaatatac gggtcgaacc gctttcgctg ctcggcacca
      121 acaccgttcc gatggattgt aacctgctgg tgatcgctgg gccagggcag acgcttcccg
      181 acccggaact gaacaagatc gagcattacc tctcccaggg tgggcggttg ctggccttgt
      241 tcaattgtta tccgctttac tcgggaaaca aggacacggg cttggagggg ttgctggcga
      301 cgtggggggt ggatgtcgga cacaacgtta tcaaagatcc ggttaacacc gtctcgggtg
      361 agaatgtgag ggtcagtagt ttcggcaagc atatcctcgt taattccctg cttctatctg
      421 agctggattt gatgctacct cgttcggtgg gccagttgaa ggggcactcc caggccgccg
      481 atgcgccgcg cgtggaagag ctcgcgtact cagggtccag ctccttcgcg ctgggcgacg
      541 tggctcacaa gcggtcgttt cctctcatgg tcgccgtcga gaagggggcc attaaggatg
      601 tcatcaccga gcgcggttct actcgcatgg tggtcgtggg tgactccctt ttcctgggca
      661 actacgaaat tgactcggga gtgaatcggg acttcgccgg caatgccgtc aactggctgc
      721 tggagcgcac gcaactgctg gagggcctcg gaccgcgtcc catcgaggaa catcgaattg
      781 taatgactag agcgcagttg catcaggcgc agtgggtctt gttagccggc ctgccggggt
      841 ctgtgctgtt gcttggcggc ctggtctggc tgcggcgcag gaaatagtct ttccgcatga
      901 accctaagaa cacttggctt tggattgccg tggcggcggg gctgtttgtg ctcgtctttt
      961 ttcaccaacg acacgcgcgc aatgccggca gcggcccgat ccgggttctg cccaacctga
     1021 aagccgccgc ggtgaccagc attcaggtcc gggcgggagc acgcacaaac atccgggccg
     1081 agcgcacgaa cggtgtttgg caacttacca aacctctcgt ttatcccgcc caggcggtca
     1141 gtatcgagag ccttctctcc cagctcgaac ggctgactcc ggcgccctac atcaccgccc
     1201 gcgaattgcg gaaccggcct aaggtggatg aggcttacgg cttcgtcgct ccccaagcct
     1261 ccatcgttat tgagcaaccc gatcacccga ttcatctctc ggttggcgcg aggacggcgc
     1321 acggcgacca ggtgttcctg caggtcgttg gcgcagacgg tgtctatgtc gtgggggccg
     1381 atcttctgaa gtacattcca agcacccctg aggattggcg cgacacgacg ctgattgatc
     1441 tgaaaggtct cgcgtttgac cggctgtccg tgactaacgg ggcgaagatc tttgagctgc
     1501 ggcgcaatgc cacgaacaag ctgtggcgca tggtgtaccc gctgcaagcg cgcgcctaca
     1561 gcgccagggc cgaagaatca ttgcaactgc tgcagagcgt cacgatagat cggtttattt
     1621 cagatgatcc caaagcggat cttgaggcgt ttggactcca gtctcccgaa cttgaggtgg
     1681 ctttcggcca aggcaccaac accgccgccc ggctccagtt cggtaagcgc ctaaccaacg
     1741 acacccggct catttacgca cggcggattg gcctgaatgg catcgttgcg gttcccaagg
     1801 acctgctcgc gccctggtac gcgctggtca acgactttcg cgatccattg ctggtcacgt
     1861 tgaccactcc ggtggcggtc attaacgtgc gcgggcaaga cagtttctcc gtgcaggagc
     1921 aaacgaatgg cacttggcga atcgtgccac aggggtttcc cgcagatgcc gggttggtca
     1981 aagacttact ttcggctttg agcaccatgc aaatcgccga gttcagggcc gataacgcca
     2041 ttgctcagga atggcctcgt tacggccttg cctctcccac gcggcaatac gccttgagat
     2101 cggcgacgac aaattctcct gcgggcccca ccaatgccct tatcgccgag ctgaatttcg
     2161 ggacgaacga ggcagacaaa gtcttcgtgc gatgcgcgga tgagcctggt ttcgtttatg
     2221 ccgtgaagct tgctgacttt gatcggctac catcggcaag ctggcagatg cgcgaacgcc
     2281 gggtttggaa tctctccacg aacgacgtgg cgcgcgcgac tatccggcag cagggcaggg
     2341 tgtgccaaat cattcggaac ggaccgtacg agtggtcgct ggcccctggt atcactaacg
     2401 atctggcggt ggaagagaca gttagcgaac tatgccaact taccgccgtc gcctgggttg
     2461 gccgcggagc cgcagctcgc gctcgctatg gcttcactga taacagtcac caaatcacgc
     2521 tcgaattaaa gaacggcgag aagagttccg tcgaattcag cagcggacct tcctccagcg
     2581 tgccatgcgc ggcggttacg ctggatgggg agccttgggt tttcgagtgc ccggcctggc
     2641 tttacggcta cgtgcagcgc tacctttctc tgccgcctaa caacccttga atatggcaac
     2701 ccgccagaaa ggccgctttt ggcggatttg ccgaatctac tttcgccggg tccgtatcac
     2761 cgtttggctg ttgattctcg ccctgctcgg tgggttattt tacctcaacc aaattggcct
     2821 tccggatttc gccaagaaac cgctgctgga gaagctgcgc gcacgcgggc tcgatctcca
     2881 gttctcccgc ttgcgcctga gctggtatca tggccttgtt gcggagaatg tccgcttcgg
     2941 gcccgcggac gaaccgctcg ggccgcacct gacgcttggt gaagtcgagt tgcgccttaa
     3001 ctacccggcc ctggcccggc ttcaagtcca ggttgactcg cttatgctgc ggaaggggag
     3061 cctggtttgg cccatcgcag aaaccaacga ggcgcctcgg catttggtgg tcgagaacat
     3121 ccagaccgac cttcgctttc tgcccggcga cgaatgggcg ctcgatcatt tcactgccgg
     3181 ctttgccggc gccaagatcc agttgtcagg ccgggttgcc aatgcgtccg ccattcgcga
     3241 atggaagttt cttggggctg aacgggttgc gcccgccaac gtgccgcgaa gccgcctccg
     3301 ccagttcgcg gacacgctgg aacgcactca tttctccgcc ccgccggagc tgagacttga
     3361 tgtgcaaggc gacgcgcgcg atctcgccag cttcggtgtc cgcgtgatgg tgattgcttc
     3421 cggcgcggac acgccctggg gcacagtcac acggggccgg tttactgccc ggctttttcc
     3481 tgccacgacc aatggacttt ccaacgccga actgactttg gaagccggcg aggcgcaaac
     3541 acgctgggcc acgatggcga atctgcatct taccgcttac ctcgcctcgt tcgaggggca
     3601 gacgaacctg gggaacggca acgtgactct ccatgccggc cacgtggata cagaatgggg
     3661 cagcgccacc aacttgcagc tcacgcttca cgccgcctcg atagaaggcc agaccaattt
     3721 ggtcaatgct gatctggccc tttcagccgg agccttcgag accaggtggg gcagtgcaac
     3781 caacgctcag ttccacgccc aatggatcca ttccctgacg aatcccgttc cgctgactgg
     3841 cgaaggcagg ctcgattgca gccaggcaac cagcgaatgg ggcgccgcgc gggagattca
     3901 gctcaatgct cgcctggcca cccccgctac ccacacctcg cctcgtgccg atgagtcctg
     3961 ggcgtggtgg gcaaaactcg aaccctacgt cttggactgg gattgccatc tgcgggggct
     4021 gcagtcgcgc gggcttgagg ccgaagacgt ggcctgcacc ggacactggc gcgcgcctga
     4081 cctgacgatc accaatcttc acgctgagct ttatcagcgg gagcttgatg ttcacgctga
     4141 cctggacgtg gcgacacgtg aactcgttct ccgcctctct tcgacccttg acccacacaa
     4201 gatcgccccc ttacttagcg agggagcgca gcgtttgctg gcgctctttt cctggccgga
     4261 gccgcccaag ttgaagggca gtctgtcgct agtcctgccg gcgtggacca atcgtcagcc
     4321 tgcctggagt gctgaagtgc tgcccacgct tcgattggag ggcgagtgcc agctcgagca
     4381 cggcggcgct taccgagacg tggcggtttc tgctgtccaa gcgcatatcc tctactcgaa
     4441 tagggtctgg cgcctgccgg acttgaagat cacgcggcct gaaggacggc tggaagccat
     4501 gcatgaggcc gatgcccgca cgggggacta ctacttcaat atcaacagca cgctcgatgt
     4561 gcgggctctg cgcccattgc tggagccggg acagcaaaag gggctcgatt acctcacctt
     4621 caccgagccg cctgcgctgg aagccgagat ccggggtaac tggcacgatg tggagcgaat
     4681 cggcattaag gggagggtgg cactgaccaa tttcactttc cgcggcgaat ccgttagcgc
     4741 ggttcaaacc gccttccaat acaccaatcg ctttctgcag attaccagtc cccggctcca
     4801 gcgtggcacg cagcaactca ccgccgacag cctcggcgca gacttcctcg cgcagaagat
     4861 ctatgtcacc aatggctaca gcacggtcga acccatggtc gtcgcgcgcg ccatcggccc
     4921 ccttatcgtc cgtgcgatcg aggcgtacca attccgacag cctccggttg cccatgtcta
     4981 tggcaccatc ccattgcatg gagaggagga cgccgacttg cattttgacc tcgtgggcgg
     5041 cccctttgac tggtggcgtt tccacgttcc gaagatccaa ggccatgtgc attggctggg
     5101 ccagcaattg actctgagca atgtcgaggt cgggttctat ggaggacaag ccactggctc
     5161 cgcacatttt gactttcatc ctcgtgatca gacggattat cagttcgccg tgaccgctac
     5221 cgacgccctc ctgcagccgt tcgcagccga tttttttctc aaaaccaatc gcctcgatgg
     5281 caggctgagc ggcacgctgg tgatttcgaa tgcgaacacg gtggacatcc agagttggaa
     5341 cggtcgcgga aacctgagct tgcgggacgg gctgatctgg gaactcccca tcttcggcat
     5401 tttctcggac gcgattaacg ggatggcgcc aggcctgggc agcagccgcg ccagcgccgg
     5461 cacctgcacg ttcggcatca ccaacggcat catccgctcc gacgacgtgg acatccgctc
     5521 gacggggatg cggctgcagt accgcggcac gctggacttc cagggccaag tcaacgcgcg
     5581 cgtggaggcc gggctcctgc gtgacatgtg gctcgtcgga ccagtcgtca gcactgtctt
     5641 gtggccggtg acaaaggtct ttgaatacaa agtcaccggt tccctgggcg acccgaaggc
     5701 tgagccggtt tacgtcccga gactcatgtt ctttcccttc cagttcccct tccatcccat
     5761 ccgcacgttg aaggggcttt tgccggagga cctcggctcc agccgcacga acgccccggc
     5821 gctcagttcg cctaaacaga actgagcaag ccaagacctc acccggctgg aatgagttac
     5881 acccctccaa cgccgccacc cgcttcggat gcctggttcg ttaccactca ctggtcggtc
     5941 gtgctgtcgg cgcgtgaaaa ggactcgccg cgctctgccg ccgcgctcga aacgctttgc
     6001 cgcacttact ggtatccgct ttacgcctac ttgcgacggc aaggccgccc tccgcacgat
     6061 gcgcaggact tgacccaggg cttcttcgcg cggctgttgc agaaggacta tttgcaagcg
     6121 gcggcgcggg agaagggcaa gttccgcact tttctactgg tggccctcaa gcgcttcctc
     6181 gcgaacgaat gggaccggga gcatgcgctg aaaagaggcg gctttgcgca ggtggtgtcc
     6241 attgatcagg aggtcgccga gtcccgcttc gcggccgagc cgtcgcacaa cgtccagccc
     6301 tacgtgctct tcgaccggca gtgggccatg acattgctgg agcggactat gacgcagttg
     6361 caggaggagt atgtcgcctc cggccgggcg aagctgttcg agtatcttca aagctgcctg
     6421 gcccgggaag agtcggccct gccttatgcg cagatcgccg cctgtctgaa cctaacggag
     6481 cctgccgtga agatggccgt ccaccggctg cgcgcccgct accgcgaaat cctgcgcgcg
     6541 gaaatcgctc acacggtctc ctccccggag gaggt
//
LOCUS       DATCGS010000469         4227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_584695, whole genome shotgun sequence.
ACCESSION   DATCGS010000469 DATCGS010000000
VERSION     DATCGS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584695"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VIL39_09665"
     CDS             <1..348
                     /locus_tag="VIL39_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5481734.1"
                     /translation="LTVPVGTNCCILIVGGWGGSLIGISSIEGMDASENETTKFTNFE
                     SGRWYRIRLRVTEKRIEGWIDKEKLVDVVTTDRRISVRPGDIELSQPFGVAAWQTMSA
                     LREIKFRRLVARE"
     gene            complement(362..949)
                     /locus_tag="VIL39_09670"
     CDS             complement(362..949)
                     /locus_tag="VIL39_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY5481735.1"
                     /translation="MSTSAVLAEKSVRRQPRWTYDRLCAELEETNQPCELWQGRLITS
                     PSPTPFHQIVAYRLQDALHRWVDPRKLGIVLGAPSDVVLAPALALQPDVLFIAKARQS
                     IIKTHICGAPDLVMEVVSPDHPRRDYKDKKEHYEAHGVKEYWIVDPGRKRIEVWTLHG
                     DFYQLAGAYSGKQTAASALLPGFNVRVDRIIAEQW"
     gene            complement(1012..2466)
                     /gene="gnd"
                     /locus_tag="VIL39_09675"
     CDS             complement(1012..2466)
                     /gene="gnd"
                     /locus_tag="VIL39_09675"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEY5481736.1"
                     /translation="MEPKADIAVIGLAVMGQNLILNMNDHGFTVAAYNRTVSKVDDFL
                     NKEAKGTKVIGAHSIEEMVGLLKSPRRVMLMVKAGKAVDEFIDQIIPHLAAGDIIIDG
                     GNSLFEDTIRRAKYVESKGFLYIGTGVSGGEEGARRGPSIMPGGSPAAWPHVKDIFQK
                     IAAKVEDGTPCCDWVGENGAGHYVKMVHNGIEYGDMQLICEAYNLMKTGLGLSADQMH
                     EVFAEWNKGELDSYLIEITRDILAFKDADGQPLVDKILDTAGQKGTGKWTVISSQDLG
                     IPITLIAEAVYARCISALKEERVAAAKKLKGPRPAIKGDPKALIEDIRRALYASKIVS
                     YAQGYMLMRAAAKERAWNLNYGGIALMWRGGCIIRSAFLGKIKEAFDKNPQLSNLLLD
                     PFFRKAIKDSQRSWRKIVSLAARKGIPVPAFSTALAFYDSYRSERLPANLLQAQRDYF
                     GAHTYERLDQPRGQFFHTNWTGKGGTTASSTYTA"
     gene            complement(2562..3068)
                     /locus_tag="VIL39_09680"
     CDS             complement(2562..3068)
                     /locus_tag="VIL39_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481737.1"
                     /translation="MLTSHELLGFMSPSLALEILTYAYETDKPLYRATLGAVAEARKL
                     RPVFLERQPRPQRHATMLATLARPALDMVSSNLIRTWLLKKYKQMLADFLDALGIVHQ
                     EGVVEDLPPTMDEAKVRAAVEILLAKYPPETAAVYLHAFNDMNEVEWPGLKAMLDSDP
                     RLQLGTAS"
     gene            complement(3166..3987)
                     /gene="lpxI"
                     /locus_tag="VIL39_09685"
     CDS             complement(3166..3987)
                     /gene="lpxI"
                     /locus_tag="VIL39_09685"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003303.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY5481738.1"
                     /translation="MTSALDTLGIIAGNRSLPLLFAKEARRQGVKRLVAVAFEGETDP
                     TLASLVDEIVWLRVGQLSKMIAAFTDRGVKQCVMTGQIAPKNLYDLRPDLRALGLLLR
                     LREKNAHTLFGAIADELKREGVELIQATPWLQLLMPASGFQLGPKLSDGQRADVEFGF
                     RIAKEVSRLEIGQIVLVKDGTVLAVEGFEGTDKCLARGGELAGKAGGAVAVKVAKEKH
                     DLRFDIPCLGPQTLETCAKARIAALAFEAGKSLLLEQDTCAQLARKHKIAVTTVG"
BASE COUNT          768 a         1179 c         1390 g          890 t
ORIGIN      
        1 cttacggtgc ccgtggggac caactgctgc atcctgattg tcggcggctg ggggggatcg
       61 ctgattggga tctccagtat tgaggggatg gacgcgtcgg agaacgagac gaccaagttc
      121 accaactttg agtcgggccg ctggtatcgt atccggctgc gggtgacgga gaagaggatc
      181 gaagggtgga ttgacaagga gaagctggtg gatgttgtca ccacggacag gcggatctcc
      241 gttcgcccgg gggacatcga actttcccag ccgttcgggg tggcggcgtg gcagacaatg
      301 tcggcgctgc gcgagatcaa gttccggcgg ttagtcgccc gggaataggc gcgccgaccg
      361 ttcaccactg ctcagcaatg atgcgatcta cccggacatt gaaccctggc agcagggccg
      421 atgcggcggt ttgcttaccc gagtatgcgc cggccaactg atagaagtct ccgtgcaagg
      481 tccagacttc tattcgctta cggcccggat ctacgatcca atactccttc accccgtggg
      541 cttcgtaatg ctccttcttg tctttgtaat cacggcgtgg gtggtcaggt gagacgactt
      601 ccatgaccaa atcgggggcg ccgcagatgt gcgtcttgat aatgctctga cgagccttgg
      661 caatgaacag cacatccggt tgcagcgcca gggccggtgc cagcacgacg tccgacggcg
      721 cgcccagaac aatgcccaac ttgcgtgggt ccacccaccg atggagggca tcctgcaaac
      781 ggtaggcgac gatctggtgg aagggtgtcg gtgaggggga cgtaatcaga cggccctgcc
      841 agagttcgca gggctgattg gtttcttcca attcagcgca caaccggtca taagtccatc
      901 ggggttgcct acgaactgac ttttcggcca aaacggcact ggtgctcatg gtcacaaatc
      961 tagggcaccc tcccacaatt tcaacggatt tgtgtgacgc ccgattcgtc cctaagcggt
     1021 ataggtgctc gaagcggtcg ttcctccctt tccggtccag ttggtatgga agaactggcc
     1081 gcggggttgg tcgagtcgct cgtaggtgtg ggcgccgaag tagtcgcgct gggcctgcag
     1141 gaggttggcg ggcagccgct cgctgcgata ggagtcgtag aacgccaggg cggtgctgaa
     1201 ggccggcacc ggaatgccct tgcgggcggc gagggagacg atcttgcgcc aggagcgttg
     1261 cgagtccttg atggccttgc ggaagaaggg atcgagcagc aggttcgaga gctgcgggtt
     1321 cttgtcgaag gcttccttaa tcttgccgag aaaagcgctg cggataatgc agccgccgcg
     1381 ccacatgagg gcgatgccgc catagttgag gttccaggcg cgttctttgg ccgcggcgcg
     1441 catgagcatg tagccttgcg cgtaggacac aatcttcgac gcatagagcg cgcgccggat
     1501 gtcttcgatg agcgccttcg ggtcgccctt aatcgcgggc ctggggcctt tgagcttctt
     1561 cgcggccgcc acgcgttcct ccttgagggc ggagatgcag cgggcataga ccgcttcggc
     1621 aatgagggtg atgggaatgc cgaggtcctg cgaggaaatg acggtccact tgccggtgcc
     1681 cttctggccg gcggtgtcga ggatcttgtc cacgagcggc tggccgtcgg catctttgaa
     1741 ggcgaggatg tcgcgggtga tctcgatgag gtaggagtcg agttcgccct tgttccactc
     1801 ggcaaagacc tcgtgcatct ggtcggcgct caggcccagg ccggtcttca tcagattgta
     1861 ggcttcgcag atgagctgca tgtcgccgta ttcgatgccg ttgtggacca tcttgacgta
     1921 atggcccgcg ccgttttcgc ccacccaatc gcagcagggc gtgccgtcct cgaccttcgc
     1981 ggcgatcttc tggaagatgt ccttcacgtg cggccaggcg gcgggcgagc cgcccggcat
     2041 aatgctcggg ccgcggcggg cgccttcttc accgccggaa acgccggtgc cgatgtagag
     2101 gaagcccttg gactcgacgt atttcgcccg gcggatggtg tcttcgaaga gcgagttgcc
     2161 gccgtcaatg atgatgtcac cggcggcgag gtgcgggatg atctggtcaa tgaattcgtc
     2221 caccgccttg ccagccttta ccatgagcat gacgcggcgg gggcttttga ggaggccaac
     2281 catctcctcg atggagtggg cgccgataac tttggtgcct ttggcttctt tgttaaggaa
     2341 gtcgtccacc ttcgagacgg tgcggttgta ggcggccaca gtgaacccgt ggtcgttcat
     2401 gtttaaaatg aggttctggc ccattacagc cagcccaatt acggctatat cagctttagg
     2461 ttccatgttg tgtatgttta ccggagtgat aaacgatact acagcgccgg gaatgcgcta
     2521 gaggaatctt ggggaggaat cttggggagg gcgctcgggc ttcagcttgc tgtgcccaac
     2581 tggaggcgcg ggtcggaatc cagcatggct ttcaggccgg gccactccac ctcattcatg
     2641 tcgttgaagg cgtgcagata gaccgcggcg gtctcgggcg ggtatttcgc gagcaagatc
     2701 tccaccgcgg cgcggacttt ggcctcgtcc atcgtcgggg gcaggtcttc gaccacgccc
     2761 tcctgatgga caatgcccag ggcgtccaga aagtcggcca acatttgttt gtacttctta
     2821 agcagccagg tgcggatcag gttgctggaa accatatcca acgccgggcg ggccagcgtg
     2881 gccagcatgg tggcgtgccg ctgcgggcgg ggctggcgtt ccaggaagac aggccgcagc
     2941 ttgcgcgctt ccgccaccgc gccgagggtg gcgcggtaga gcggtttgtc tgtttcgtag
     3001 gcgtaggtaa gaatttcgag cgccagggaa ggcgacatga aacccaacaa ttcatgtgac
     3061 gtgagcattt gcttgcgtga gaggttgaag gggttaaata agttaaatga gttaaaacgt
     3121 taccagggtt aaatattgtg gttcgcgaat cttaggcggc tggcattatc caactgtcgt
     3181 cacggcgatc ttatgcttgc gcgcgagctg ggcgcaagtg tcttgttcca gcaggagaga
     3241 tttgccggcc tcgaacgcga gcgcggcaat tcgagccttg gcgcaggtct ccagcgtttg
     3301 cgggccgagg cagggaatgt cgaagcggag atcgtgcttt tccttcgcga ccttgacggc
     3361 cacggcgccc ccagcctttc cggccagctc gccgccgcgg gcaaggcatt tgtcggtgcc
     3421 ctcaaaacct tcaaccgcca ggacggtgcc gtccttgacc aggacaatct ggccgatttc
     3481 caggcgggag acttccttcg cgatgcgaaa gccaaactcc acgtccgccc tttgcccatc
     3541 cgacaacttc ggtccgagct ggaagccgct cgccggcatc aagagttgca gccagggggt
     3601 ggcctggatc agctcgacac cctcgcgctt cagttcatcg gcaatcgcgc cgaagagcgt
     3661 gtgggcgttc ttttcgcgca agcgcaagag caggccgagg gcgcggaggt cggggcggag
     3721 gtcataaagg ttcttcgggg cgatctggcc cgtcatcacg cactgcttaa cgccgcggtc
     3781 ggtgaaggcg gcgatcatct tcgagagctg cccaactctg agccagacga tttcgtccac
     3841 cagcgaggcg agggtggggt cggtctcccc ttcaaaggcg acggcgacga gccgcttgac
     3901 cccctggcgg cgcgcttctt tggcgaagag cagcggcagt gaacgattgc cggcgataat
     3961 ccccagggtg tccaacgcgc tggtcatggc tgcttgggga ggtcccgcag gtgaacatgc
     4021 gagccctgtg gaatgggtaa acctggaatc gaccgcttac gcctgtcggc ggcggcttca
     4081 cccgggcctg cactcaggca gtgtcccata atctcgcgca tttctcctat ttaagggcaa
     4141 gacctgcgac ttgtaaactt ggatactact tgctctgatc tggcttgtgg tctgccggtt
     4201 gcacgacatt ggtgccctga ggtttcg
//
LOCUS       DATCGS010000470         3578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_535843, whole genome shotgun sequence.
ACCESSION   DATCGS010000470 DATCGS010000000
VERSION     DATCGS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535843"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..2025
                     /gene="rpoD"
                     /locus_tag="VIL39_09690"
     CDS             154..2025
                     /gene="rpoD"
                     /locus_tag="VIL39_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5481739.1"
                     /translation="MNNRAKNHGGGSERTASGKTPRPRRAFVFGAKTAGREESHPKKA
                     RSSGIEAGGVAPGKVLPTSNGGNLAAGARPEAMDLTEAIKVLVQVAREQSHLTYDDVN
                     ELLPDGVTPEDLDALYTKLHNLGIEIVAHVEMEKAKPEEPEPEEDRRFDALDDPVRMY
                     MNQMSRVPLLTREQEVEVFKRIEEAELEMKRLVYGLGFAAKEHCAIAEKLLADPPKER
                     FDRIVVDHKIATREGYLKDLRRLVKKIRALDAQTDELYARLQKSVSTGQRERLLREYE
                     KLNRNLQAAFPKFFYKQKMLEDMIAVASNVHEKFRAGSRHIQELEEHRKSSHQQAALR
                     EERDKLHALEQFVRRPQEEFLETFRQLHGAAERAHQAKAHMAEANLRLVVSIAKKYVN
                     RGQAFLDLIQEGNIGLLKGVEKFEYRRGYKFSTYAVWWIRQALTRSIADQARTIRIPV
                     HMIEIMSRLWRAQKQISQELGREPTAEDLADEMRLPVSRINALLKMARQPVSLDAPVG
                     DDGDVNVGDFIEDKGAENPSEGTSHSMLKEKLDHVLTSLSERERKILEMRFGLVDGFG
                     HTLEEIGNLYNVTRERIRQIEAKALRKLRHPTRAHHLHGFLENQQEAVSSATPTVAP"
     gene            2054..3523
                     /locus_tag="VIL39_09695"
     CDS             2054..3523
                     /locus_tag="VIL39_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY5481740.1"
                     /translation="MAARDLLLDPIWKGEELGRPIPASPHAVSVALPRWQDVVGYEEK
                     KPEVVNALTSGYPRFFIHAKVQELARQIGDGRPCLPFPSLRAAQQCAEFIEHTGGARA
                     KVITFRGVHGVVTNDAGQPALRAFWQHTGLIVSTREAEAHLGGPAPAPDDLDVRQSLR
                     RQLAGFYDCAEDDVFLTPTGMAAQFAALRAVMQRTPEQPTAQLGFPYVDTLKLQQKLG
                     HGGILLHHLGTIESELQALLRRQPLAACFCEIPGNPLLGSADVRTISPILRGHRVPLV
                     VDDVVATPANVDLSGHADLIATSLTKFILGTGNAMGGALICNPKSPLYAELKPIVSDQ
                     HEELLWGEDAVVLEAQARGFPERMKRHNQNGLLLAERLRRHPAVERVWYPKWEFSEAY
                     EAVRRPNGGWGALITFLPRNGAEKSPGIYDRLAFCKGPSLGTVFTLACPFTLLAHYTE
                     LEWAESCGVSRYLIRISVGLEDPEDLWQRLERALTEPLA"
BASE COUNT          725 a         1099 c         1133 g          621 t
ORIGIN      
        1 aatgacgaac tatcgcctgc cgtaactcgt tcagtcatta gtctgagcca gcccagcaaa
       61 cggtttcgtc cccgatgtgg ggacgtgaaa gcgcacgtaa tccattgggt atccacggtc
      121 aacggctgag ttttgccagt gaaggaaagc aaattgaata acagagcaaa gaatcatggc
      181 ggcggcagcg agcgaacggc ctcgggcaag acgccgcggc cccgccgcgc atttgtcttt
      241 ggagccaaga ccgctggccg cgaggaatcg catccgaaga aggcccgcag tagcgggatt
      301 gaagccggcg gtgttgcgcc cgggaaggtc ctgcccacca gcaatggcgg caacctcgct
      361 gctggcgcgc ggcctgaggc gatggactta acggaagcca tcaaggtttt ggtccaggtg
      421 gcccgcgagc agagccatct tacctacgac gatgtcaatg agcttctgcc ggatggtgtt
      481 acgccggagg atttggacgc gctctatacc aagctccata accttggcat cgagattgta
      541 gctcatgtcg agatggagaa ggccaaaccc gaggagccgg agccggagga agaccgccgg
      601 ttcgatgccc tggacgaccc tgttcggatg tacatgaacc agatgagcag agtgcccctg
      661 ctcacgcgcg agcaggaggt ggaggttttc aagcggatcg aggaggccga actcgagatg
      721 aagcgtttgg tttatggcct gggcttcgct gccaaggagc actgtgcgat tgccgaaaag
      781 ctgctggccg accccccgaa ggaacgtttc gaccgcatcg tagtggacca caaaatcgcc
      841 acgcgcgaag ggtatctcaa ggacctccgc cggctggtga agaagattcg ggcgttggac
      901 gcgcagacgg acgaactgta tgcccggttg cagaagtccg tgtccactgg ccagcgcgaa
      961 aggcttctgc gcgagtacga gaagctcaac cggaatctcc aggccgcctt tccgaagttc
     1021 ttttacaagc aaaagatgct tgaggacatg atcgccgtag ccagcaatgt ccacgagaaa
     1081 ttccgagccg gctcgcggca tatccaggag ctggaagagc atcgcaaaag cagccaccag
     1141 caagccgccc tccgggagga acgagacaag ctccacgccc tcgaacaatt cgtccgccgg
     1201 ccgcaggagg aatttcttga gacctttcgt cagttgcacg gcgctgccga acgcgctcat
     1261 caggccaaag cccacatggc cgaagccaac ctgcgcctcg tggtctccat cgccaagaaa
     1321 tacgtcaatc ggggccaggc ctttctcgat ctgattcagg aaggcaatat cgggctgctc
     1381 aagggggtcg agaaattcga atatcggcgc ggctataagt tttccaccta tgcggtgtgg
     1441 tggattcgcc aggcgctgac ccgctcgatt gccgaccagg ctcgcaccat tcgcatcccg
     1501 gtgcacatga tcgagatcat gagccggctc tggcgggcgc agaaacagat cagccaggag
     1561 ttggggcgcg agcccacggc ggaagatctc gccgatgaaa tgcgtctgcc ggtttcgcgc
     1621 atcaacgcgt tgctcaagat ggcccgccaa ccagtctcgc ttgacgcgcc ggtgggtgac
     1681 gatggggacg tgaacgtggg ggacttcatc gaagacaagg gcgccgagaa cccgtccgaa
     1741 ggaaccagcc atagcatgct caaagagaag ctggaccacg tgctgaccag cctcagcgaa
     1801 cgggaacgga agatcctcga aatgcgcttt gggctggtgg atggcttcgg ccacaccctc
     1861 gaggaaattg gcaatctgta caacgtgacc cgcgagcgca ttcgccagat cgaagcgaag
     1921 gctttgcgca aactgcggca cccgacacgg gctcaccacc tgcacggctt cctcgagaac
     1981 caacaggagg ccgtctcgag tgccactccg actgtagccc cctgagcgag cgccgcagca
     2041 ttaaccggta ataatggctg cgcgtgatct attgctagac ccgatctgga agggcgagga
     2101 gcttggacgc ccgattccgg cctcgccgca cgcggtgtcg gtggcactgc cgcgctggca
     2161 ggatgtggtg ggttacgagg agaagaaacc cgaggtggtc aacgcgctca ccagcgggta
     2221 tccgcgcttc ttcattcatg cgaaggtgca ggaactggcg cggcagattg gagacgggcg
     2281 gccgtgcctg ccctttccgt cgctgcgggc tgcccagcaa tgcgcggaat tcatcgagca
     2341 caccggcggc gcacgggcca aagtaatcac cttccgcggt gtgcatggcg tcgtaaccaa
     2401 tgacgccggc cagcccgcct tgcgcgcctt ctggcagcat acggggctta ttgtttccac
     2461 ccgggaggcc gaggcgcact tgggtgggcc ggcgccggct cccgatgatt tggacgttcg
     2521 ccagtccctg cggcgacagc tcgccgggtt ctacgactgc gcggaggacg acgtgttcct
     2581 gaccccgacg ggaatggccg cccagtttgc cgctctccgg gcggtcatgc agcggactcc
     2641 cgagcagccc acggcccagt tggggtttcc ttacgtggac actttgaagc ttcagcagaa
     2701 gctcggccac ggcgggatac tgctgcacca cctgggaacc attgagagcg aactgcaggc
     2761 cctgctgcgc aggcaaccgc tggcggcgtg cttttgcgaa atccccggca atcccctgct
     2821 cggctcagcc gacgtgcgca cgataagccc gatcctccgc gggcatcgcg ttcccctggt
     2881 ggttgacgac gtggtcgcca ccccggccaa cgtggacctg agcggtcacg ctgacctgat
     2941 tgcgaccagc ctcaccaagt tcatcctcgg caccggcaac gcgatgggcg gcgcgctcat
     3001 ctgcaatccc aagtctcccc tttacgccga gctcaagccc atcgtcagcg accagcacga
     3061 ggagttgctg tggggcgagg atgccgtggt gctggaggcg caagcccggg gcttcccgga
     3121 gcggatgaag cgccataatc agaacggcct gctgctggcc gagcggttgc gccgtcatcc
     3181 ggcggtcgag cgcgtctggt atcccaagtg ggagttcagc gaagcctacg aagccgtgcg
     3241 gcgtccgaac ggcggctggg gcgcgctcat caccttcctg cccaggaacg gggcggagaa
     3301 gtcgcctggc atctacgacc gtctcgcctt ctgcaaaggg ccgagcttgg gcacggtgtt
     3361 tacgctcgcc tgcccgttca ccctgctggc gcattatacc gaactggagt gggcggaatc
     3421 ctgcggcgtc tcccgttacc tcattcgcat ctcagtcggc ctggaagacc cggaagacct
     3481 ctggcagcgg ctcgaacggg ccctgaccga gcccttagcg taagcagtcg cgccggcggg
     3541 tccggcaacc ctatcgggaa ccatcgagtg cgcgactc
//
LOCUS       DATCGS010000471         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_486462, whole genome shotgun sequence.
ACCESSION   DATCGS010000471 DATCGS010000000
VERSION     DATCGS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486462"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIL39_09700"
     CDS             complement(<1..560)
                     /locus_tag="VIL39_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY5481741.1"
                     /translation="MQKPLLTFPILIAAGLISLCGPDCAGELGLPNGFTRLKQLPAPK
                     IVASTEAYPGGNHNVGNIIDGKAQTEYSSNSKGTNTFIEFDLGAPTSVAAFRHVDRND
                     PATIASSELRFLDGSGSVVSTAPVKHVNQRGGVTFLTLPAPVTARRVRWQVTGLGAGY
                     GTVGGAEIAFFTAGETESLPRGITIE"
     gene            complement(629..2557)
                     /locus_tag="VIL39_09705"
     CDS             complement(629..2557)
                     /locus_tag="VIL39_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481742.1"
                     /translation="MKQTFIAGVLGALIWASFLAEAGAGTRVIPHPLPSHPGNIFLVG
                     ETVTVPALPAGDGDAWRVVNYEGQTVAEGRVKAGPVELGRLPVGYYEIWRSAAHLTNR
                     LSLGVLEGLRAPTPASSPIGIDVAMAWFYPEEKMGSVANLCALAGINHVRDRLSWPEL
                     EPKKGEFAPTNRYDFSAEAQSAAGLQVLQVGHSSPAWANPNGKRFPLDLRDTYNFHRA
                     LAQRWQGEVVAFEPWNEADISMFGGHTGSEMAALQKAAYLGLKAGNPKVIACLNVFAS
                     HRAATLRDLHENEAWPYFDTFNLHHYDRFEAYPRLYAAFRAVSAGKPLWVTECSVPVK
                     WRGDERLKEPTDEDLRVQGERLTMTYALALHEGAQAVFYFMLPHYVEGETQFGVLRPD
                     LTPRPAFVALAAVGRLLADAKPLGRLKTGSDTVCAYLFQARPDGQRADVLVAWAERET
                     EFALPKPPRACFDHLGRARESTSKVLKLSRAPLFVLLAGGTRLPLVSPPSRPKLLPGK
                     PSPVVLQGVLPEASILLEKSAYKIPAGQTTTIPIFLYNFGGKPAHGRLSTSVSFEAAA
                     SPTPAPWGIELPREVEVAPGERKELPLRVTCASTKDVDAAAIRITGEFGGAGKPMLSL
                     RLVPIAESATSGKAGSLK"
BASE COUNT          491 a          855 c          834 g          493 t
ORIGIN      
        1 ctctcgatcg taataccgcg aggaagtgac tcggtttccc cagcggtgaa gaaagcgatc
       61 tccgccccgc cgacggtgcc atagcccgcg cccaacccgg taacctgcca gcgcacgcgc
      121 cgagcagtca ccggcgcggg cagagtcagg aacgtcaccc cgccgcgctg gttgacatgc
      181 ttgaccggcg cggtgctgac cacgctgccg gagccatcca ggaagcgcag ttcggaggat
      241 gcgattgtgg ccgggtcatt gcggtcaaca tggcggaagg ctgccacact ggtgggcgcc
      301 cccaggtcga attcgatgaa ggtgttggtg cctttggagt tcgaggaata ctcagtctgg
      361 gccttgccgt caatgatgtt gccgacgtta tggttcccgc ccggataggc ttccgtgctc
      421 gcaacaatct tgggtgccgg cagttgcttc aggcgagtga agccgttcgg cagccctagc
      481 tcccctgcgc aatcaggacc gcacagcgag atcagaccgg ccgcaatcag tatcgggaac
      541 gtcagcaatg gcttttgcat aggcttttgt ttagtggctt gcccggggtt gcagggcgag
      601 gtgattccgt ttggctctga gccgttcatc acttcaaact gccggctttg ccggaggttg
      661 ctgactccgc gataggcacc aaccgcagcg agagcatggg cttgccggcg cctccgaact
      721 cgccggtgat ccgaatcgca gcagcatcaa cgtctttcgt gctcgcacag gtgacgcgca
      781 gtggcagttc cttccgctca ccgggcgcaa cttcgacttc gcggggaagt tcgatgcccc
      841 acggcgcagg tgtgggcgac gctgcggcct cgaaggacac ggatgtgctc agtcgcccat
      901 gggccggctt cccgccgaag ttatagagga agatcggaat cgtcgtcgtt tggccggctg
      961 gaatcttgta ggcggacttt tccaagagaa tgctcgcctc cggcagcacg ccttgcagca
     1021 ccacgggcga gggtttgccc ggcagcagtt tgggccgact tggcggcgag accagcggga
     1081 ggcgcgtccc tcccgccagt agcacaaaca gcggcgcgcg gctcagtttg agcaccttgc
     1141 ttgtgctctc acgggcgcga cccaggtgat cgaaacaagc cctcggcggc ttcggcagtg
     1201 cgaactccgt ctcacgttcg gcccaggcaa cgaggacgtc cgcacgttgg ccatcaggtc
     1261 tggcttgaaa gaggtaggcg caaactgtgt cgcttccggt cttcaaccgc ccgagcggtt
     1321 tggcatccgc cagcagtcgg cccactgccg ccagcgccac aaacgccggt cgcggcgtca
     1381 agtccgggcg cagcacgccg aactgggtct cgccctcgac gtaatgcggc agcatgaagt
     1441 agaagaccgc ctgcgcgcct tcgtgcaacg ccagcgcgta agtcatggtc agccgttcac
     1501 cttggacgcg caaatcttcg tcggtgggtt ctttcaaccg ctcatctcca cgccatttca
     1561 cgggcacgct gcactccgta acccacagcg gcttgcccgc cgagacggcg cggaaggccg
     1621 cgtaaagccg cggataagcc tcaaagcggt catagtggtg caggttgaag gtatcaaagt
     1681 aaggccaggc ctcgttttcg tgcaggtcgc gcagtgtcgc cgcacggtga gatgcgaaca
     1741 cattcagaca ggcaatgacc ttcgggttgc ccgctttgag gccgaggtaa gccgcctttt
     1801 gtagcgcggc catttcgctg cccgtatggc ccccaaacat cgagatgtcc gcctcattcc
     1861 acggctcgaa cgcgaccacc tctccctgcc agcgttgcgc cagggcgcgg tggaagttgt
     1921 aggtgtcgcg caaatccagc gggaaccgtt tgccattcgg gttcgcccac gccggcgagc
     1981 tatggccgac ctgcagcact tgcaatccag ccgcggattg ggcctcggcg gaaaagtcgt
     2041 aacggttggt cggcgcgaac tcccccttct tcggctccaa ttccggccag ctcagccggt
     2101 cgcgcacgtg gtttatgccg gccagcgcgc acagattggc cacgcttccc atcttttctt
     2161 cggggtagaa ccaagccatc gccacgtcaa tgccgatggg cgaactggcc ggcgtcggcg
     2221 cgcgcaatcc ttccagcacc ccgagggaga gacgattagt gaggtgtgcc gcgctgcgcc
     2281 agatttcgta gtaaccaacc ggcaggcgcc ccagctcgac cggtccggct ttgacccggc
     2341 cttccgccac cgtttgtccc tcgtaattca ccacccgcca ggcatcgccg tcaccagcgg
     2401 gcagcgcggg gacagtaacc gtctccccga ccaggaagat gttgcccggg tgagatggca
     2461 gcggatgggg aatgacgcgg gtgccggcgc cggcttcggc caggaaactg gcccaaatca
     2521 acgccccaag gactccggcg ataaatgtct gtttcattga acctgctgcc cgcaagagaa
     2581 cgccgccaga ccgggcaagt caagcggcgt gcgagaaata cgtgaaatat aacgcagttg
     2641 ccttggtctc gactccccgg aaggcgtcaa tgc
//
LOCUS       DATCGS010000472         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_235138, whole genome shotgun sequence.
ACCESSION   DATCGS010000472 DATCGS010000000
VERSION     DATCGS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235138"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..446)
                     /locus_tag="VIL39_09710"
     CDS             complement(204..446)
                     /locus_tag="VIL39_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY5481743.1"
                     /translation="MKIPRDCSGAELVRALRHLDYETRRQTGSHIQLATQRGGEHPVT
                     VPNHHPLKVGLLHGLLKDIAAHHNLTVEQLLCELGL"
     gene            complement(443..652)
                     /locus_tag="VIL39_09715"
     CDS             complement(443..652)
                     /locus_tag="VIL39_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HEY5481744.1"
                     /translation="MSEIIFEVREAEEGGLWARALGYCIFTQGEDWEDLRKMVKDAVA
                     CHFEQESERPKIIRLHYVRDEVLAA"
     gene            756..2168
                     /gene="hemG"
                     /locus_tag="VIL39_09720"
     CDS             756..2168
                     /gene="hemG"
                     /locus_tag="VIL39_09720"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY5481745.1"
                     /translation="MKSIAIIGAGITGLTTAFCLKRKGVPVTVYEASDRVGGAIQSLR
                     TQGYLAEFGPNTILETSPKVTQLICDAGLASRRLDPDPRASARYVVRYQRPIEMPGSP
                     LGFFTTKLFTARAKLAVLREPFVPARRDGKEESVAEFVVRRLGQEFLDHAIDALVSGV
                     YAGDPYKLSVPQAFPKLGQLEARYGSLIKGQIFGARERKRRGEIAKDRAPKFSFDEGL
                     QVLPDTLRERLGEAVRLNTAVTRLTQTPEGWTLDLREGGQQTRAEHSAVIYAGTAFKL
                     AELEVQATVPLHLATFAAIRYPPVVSVVLGFRREDVVHPCEGFGMLIPKVAGFKILGT
                     IFSSSLFPNRAPAGHLTLTSYVGGERYPELASLPPEKLYALTCEDLRVLLGVRGKPTF
                     QHSVYYPKAIPQYNVGYGRYRELMTEIEGKAPGLFLAGHYREGISLSDTIVSACNVAD
                     RAGEFLRVAPAGRPEASVAA"
     gene            2178..3236
                     /gene="hemH"
                     /locus_tag="VIL39_09725"
     CDS             2178..3236
                     /gene="hemH"
                     /locus_tag="VIL39_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5481746.1"
                     /translation="MSKGVLLVNLGSPDSSSVADVRRYLREFLMDGRVLDVNWLLRFC
                     IVNFAILPSRPKQSAEAYQKIWTPAGSPLVVTSRNVQMKLQRRLAIPVELAMRYQHPS
                     IPQAVRNLEQKGVDEVLLVPLFPHYAISSFETAVERVKEVAAALAPQLRVQVQPPCFD
                     HLDYIAALVASAQELLQRGYDHLLFSFHGLPERHMRKADPTGRHCLATENCCETASPA
                     HATCYRAQCFKTVAAFVKQASVPKDKYSVAFQSRLGRDPWLKPYTDFELPRLAERGVK
                     KLLVICPAFVSDCLETLEEIGLRGRESFLAAGGTDFALIPCLNEHPLWLTALEGMVRR
                     FMASDRGQAGPKAVEAAR"
     gene            complement(3351..3887)
                     /locus_tag="VIL39_09730"
     CDS             complement(3351..3887)
                     /locus_tag="VIL39_09730"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY5481747.1"
                     /translation="MAKYTKFEELPVWKEAARLYNRVLDLLEEPGVPLTPGFRNQLDR
                     AALSVSNNVAEGFERVTTNELLAFLAIARGSGGEVRSMMAVVKDRPRLKPHIRHLQDI
                     RALAESCARQLTAWTGSLEASPVQGKRHLAAPERERREIADKARSFRVNFLRNLKPNH
                     PLYSSPEARAARGESVPE"
BASE COUNT          737 a         1253 c         1332 g          828 t
ORIGIN      
        1 gctggatgac atggaagccg acgggctgct ggtgaaaacg ccagaggggc tggttgtgac
       61 cgatctggga cggctgctga tccggaacat tgcgatgcgg tttgatgcct actcgacgac
      121 gcgaagggag aaccggttct cacggacgat atgaaatccg tcgcaattat cggtgccggc
      181 atcacgggcc tgaccgccgc catttatagc cccaattcgc acaacaactg ctcgaccgtc
      241 aggttgtgat gggcggcgat atctttgagc aaaccgtgga gcaaacccac cttgagcggg
      301 tgatgattgg gaaccgtgac agggtgttct ccgccacgtt gagtggccag ttggatatgc
      361 gagccggttt gccgccgcgt ctcgtagtcc aagtgccgaa gcgcacggac cagttcggca
      421 ccactgcaat cccggggtat cttcacgccg cgagcacttc atcccggacg taatggagcc
      481 tgataatctt gggtcgttcc gactcctgtt caaagtggca agcgaccgcg tccttcacca
      541 tcttgcgcag gtcctcccaa tcctcgccct gggtgaagat gcagtaaccg agcgctctcg
      601 cccaaagccc gccttcctca gcttcgcgca cctcgaaaat gatttcgctc atgcagacag
      661 cttcccagaa gtcaggatag gtttcaagcc gcagttttct ggccggtcgc tctcgacaaa
      721 gtcagccgcg cgcctagatt gaacggcagt ttggcatgaa atccatcgca atcatcggtg
      781 ccggcatcac tggcctcacg accgcctttt gcctgaagcg caagggcgtg ccagtcacgg
      841 tctatgaggc cagcgaccgg gtcgggggcg cgatccagtc cctccggacg cagggttacc
      901 tggcggagtt tggccccaat acaatcctcg aaacctcgcc gaaggttacg cagcttattt
      961 gcgacgccgg gctggcgtca cggcggcttg atcccgatcc gagagccagc gcgcgctatg
     1021 tcgtgcgcta ccagcggccg atcgagatgc cggggtcgcc cctgggcttc ttcacgacca
     1081 agctctttac cgcgcgggcc aagctggccg tgctgcgcga gccgttcgtg ccagcgcgcc
     1141 gcgatggcaa ggaagagagc gtcgctgagt tcgtcgtgcg ccgcctcggg caggagtttc
     1201 tggaccacgc catagatgcg ctcgtttccg gcgtgtatgc gggtgaccct tacaagctct
     1261 cagtgccaca agcgtttccg aagctgggcc agcttgaggc gcgctatggc tcgctcatca
     1321 aagggcaaat cttcggcgct cgcgaacgca agcggcgcgg ggaaattgcg aaagaccgcg
     1381 cccctaagtt ctcctttgac gaagggttgc aggtgttgcc cgataccttg cgtgagcggt
     1441 tgggcgaggc cgtgcgcctt aacaccgccg tcacccggct cacgcagacg cccgagggtt
     1501 ggacgctcga cttgcgcgag ggcggccagc agacgcgggc cgagcacagc gcggtgatct
     1561 acgcgggcac agccttcaag ctggcggaac tggaggtcca ggcgaccgtg ccgctccacc
     1621 tcgcgacctt cgcggcgatt cgctatcccc ccgtggtcag tgtcgtgctg ggcttccggc
     1681 gcgaggacgt cgtgcatccg tgcgaagggt ttggcatgtt gataccgaaa gttgcgggct
     1741 tcaagatcct gggcaccatc ttctcgtcct ccctgtttcc gaaccgcgca ccggcgggac
     1801 atctgacgct gaccagctat gtcgggggtg agcgataccc ggagctggcg tcgctgccgc
     1861 cggagaagct ctacgcgctg acgtgcgaag acttgcgcgt gctgctgggc gtgaggggca
     1921 agccgacctt ccagcacagc gtgtattatc cgaaggcgat cccgcagtac aacgtcggct
     1981 atggccgcta ccgtgagctg atgactgaaa tcgaggggaa agcgcccggg ttgttcttag
     2041 ctggccacta ccgcgagggc atttcgttaa gcgatacaat cgtgtccgcc tgcaacgtgg
     2101 cggacagggc aggggagttc ctaagggtgg cgcccgcggg ccggcctgag gcgtcagttg
     2161 cggcatgaga ctgcacgatg agtaagggag tgcttctggt caacctgggg tcgccggact
     2221 cgtcgtcggt ggcggacgtg cggcgctatc tgcgcgagtt cctgatggac gggcgcgtgc
     2281 ttgacgtcaa ctggctgctc cggttctgca tcgtgaattt tgccatcctg ccctcgcgcc
     2341 cgaagcagtc ggccgaggcg tatcagaaga tatggacccc tgccggctcc ccgctcgtgg
     2401 tcaccagccg caacgtgcag atgaagctcc agcggcggct tgcgatcccg gttgagttgg
     2461 cgatgcgtta tcagcatccg tccattccgc aggcggtgcg caacctggag caaaagggcg
     2521 tggacgaggt tctgctggtt cccctgttcc cgcattatgc gatctccagt ttcgagaccg
     2581 cggtggagcg ggtgaaggaa gtcgctgctg cgctcgcgcc gcaattgcgg gtgcaggtgc
     2641 agccgccatg ttttgaccat ctcgactaca ttgcggcact cgtggccagc gcgcaggagc
     2701 ttctccagcg gggctacgac catctgctgt tcagtttcca cggcctgccg gagcgccaca
     2761 tgcgcaaggc agatccgacc gggcgccact gtctggcgac cgagaactgc tgcgaaacgg
     2821 ccagcccggc tcatgccacg tgttaccgcg cgcagtgttt caagacggtg gccgccttcg
     2881 tcaagcaagc cagcgtgccc aaggataagt attcagtcgc gtttcagtcc cggctggggc
     2941 gtgatccctg gctgaagcct tacacggact tcgagcttcc ccggctggcg gagcgcggcg
     3001 tcaagaagct gctggtgatt tgccctgcct ttgtctccga ctgcctggaa acattggagg
     3061 agatcggcct gcgtgggcgc gagagctttc tggcggcagg cggaacggat tttgccctga
     3121 ttccctgcct aaacgagcac ccgctttggc tgacggcatt agaggggatg gtgcgccgtt
     3181 ttatggcctc cgacaggggc caggccgggc cgaaagcggt tgaagcagcg agatgaggga
     3241 ggcggattat cgatccgccg catgatggcc gataggcaat agccgatggc caatgcgcca
     3301 acgctcgctt ttggcgagtg gtcggctgcc aacggtccgc ctgttgcctg ctactccgga
     3361 accgattcac ctcgggcggc gcgggcttcc ggggaggagt agagcggatg gttaggtttg
     3421 aggttgcgga gaaagttcac ccggaagctg cgggccttgt ctgcaatctc gcgccgctcg
     3481 cgttccgggg cggcaaggtg acgctttccc tgaacgggcg aagcttcgag ggaaccggtc
     3541 caggcggtca gttgccgcgc gcaggattcc gccagcgcac ggatgtcctg caagtgccgg
     3601 atgtgcggct tgagtctggg cctgtcctta accaccgcca tcatcgagcg cacctcgccg
     3661 ccggagcccc gcgcaatggc cagaaaggcc agcaactcat tcgtggtcac gcgctcgaat
     3721 ccctcggcga cgttgttgga aacggacagc gcagcgcggt cgagttggtt gcggaagccg
     3781 ggcgtaagcg gcacgccggg ttcttccagg agatcgagga cgcggttgta gagccgcgct
     3841 gcctccttcc agacggggag ttcttcaaac ttggtgtatt tggccatgca gtattctcct
     3901 ttcagttgat ttgatttggc tattggttgg ctattggcta ttgcctattg gcgattcttg
     3961 attggcggtc tcgctcatcg ccactgctta tactcccgtc tccatttccc tgaaaagcca
     4021 aaagaaaagc gggcccgctg cgcggagtcc gtctcccacc gtcgctgacg caccaccaga
     4081 aaagtgcgat tccctttgaa tagccgccaa accattaccg tcaatactcg gcacgcgtcc
     4141 ggtcgagacg
//
LOCUS       DATCGS010000473         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_733229, whole genome shotgun sequence.
ACCESSION   DATCGS010000473 DATCGS010000000
VERSION     DATCGS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733229"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VIL39_09735"
     CDS             <1..1213
                     /locus_tag="VIL39_09735"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY5481748.1"
                     /translation="LLFTDYFELHWGLFLCGLLFLLVCATDRTSSPSTLDPRSSRFGS
                     SWRWTACALTLLVFAGLDRLLAWLPEQARTVPKGYFIGLRIGMWTIVALLVASWIARK
                     KFRNFQYWRFVTCMWLFLGLLGLGRTLWTQANESSSDRVYRSRNFYGVLTVYEHRKAE
                     PLSHYFLLQHGRITHGLQFADPVQATWPISYYGKDSGIGLGVGALPPGNRRIGVVGLG
                     TGTMAAFGRAGDYLRFYEINPEVEAVATTWFSYLPKCLGNVEVAPGDARLTLEWEPPQ
                     HFDLLVLDAFSSDAIPVHLLTKEAFQTYERHLNTNAIIAVHISNHYLNLEPVVVNLAR
                     HFGYKLASIDYEEDEEEWWLYGSTWILLSRSEQIMNRPDIRNAASTVNTNSVKVPLWT
                     DDFASLFQILR"
     gene            1227..>2722
                     /locus_tag="VIL39_09740"
     CDS             1227..>2722
                     /locus_tag="VIL39_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEY5481749.1"
                     /translation="MSTTPQSPPNPFLNSQATPFAGRPSPFAVQPALLAFLICLVLGL
                     AAVGWRATVRAKDARARAGLEALARGAAVELQFSQVVSAAEVLGALARQHGGAIPDFQ
                     KLAADLLVARPALASLELQPGGIVSDIVPRAANARAVGFNVLNNPTYRPGASATLQRR
                     RLTVTGPLALYSGEPGIVARVPIFQRGRDGREAFWGFVAVSMRVSEALRWAQVDDLAR
                     HGYNYAFSVAASRREKGLTLAAHGALSAQAAVQQPVRAQDVTFRLMLQPRGGWVNKTK
                     LVLELVAVLVASGLVCLLVNLLESRRAVELALTDANRRLTGETEDRKQAQADCHVARN
                     EAAAAQAELNRTRSALKSSNEQEVRLSVSVRTAEAAAQAMQAELDQARMALQQAEQSA
                     EPSPVSVSEGQDTHPELIAPPEAHDAPASSSEPAPEAVPTPATTPEPESPAPSIPAEP
                     PPPATALPAEPPTEKKPAKVPRRKKARRNNQIDLFAPQPTPEPTPAMPP"
BASE COUNT          474 a          911 c          846 g          491 t
ORIGIN      
        1 gctgctgttc acggattatt tcgaactgca ctggggcctt tttctttgcg ggctattgtt
       61 cctgctcgtt tgtgctaccg accggacttc ctccccctcg accctcgatc ctcggtcctc
      121 gcgcttcggc tcctcctggc gctggacagc ctgcgcgctg accctgctgg tgttcgccgg
      181 cctggaccgg ttgctggcgt ggctgcccga gcaggcgaga accgtcccca aaggctactt
      241 catcggcctg cgcatcggga tgtggacaat cgtggctctg ctggtggcgt cctggatcgc
      301 gcgcaagaag ttcaggaact tccaatactg gcgcttcgta acgtgtatgt ggttgttcct
      361 gggtctgttg ggattgggca ggaccctctg gacgcaggcg aatgagtcca gcagcgacag
      421 ggtttaccgc tcgcgcaatt tctacggtgt gctgaccgtg tacgagcata ggaaggctga
      481 gcccttgtcc cactatttcc tcctgcagca cgggcggatc acccacggcc tgcaattcgc
      541 tgaccccgtc caggccactt ggccaatcag ctattacgga aaggacagcg gcatcgggtt
      601 gggtgtgggc gcgctgcccc ctggcaatcg gcgcatcggc gtggtcgggc tcggcaccgg
      661 cacgatggcg gcttttggcc gcgcgggcga ttacctgcgg ttctacgaaa tcaaccccga
      721 ggtggaggcg gtggcgacca cttggttcag ctacctcccc aagtgtctgg gcaatgttga
      781 agtcgccccc ggcgacgccc gtctcacgct ggagtgggag ccaccgcaac acttcgacct
      841 gctggtcttg gatgctttca gcagcgacgc catcccggtg cacctgctga ccaaggaggc
      901 cttccagact tacgagcgtc acctcaatac caacgccatc atcgccgtcc atatctccaa
      961 ccattacctc aatctggagc cggtggtcgt caatctcgcg cgccacttcg gctacaagct
     1021 ggcgtccatt gattacgagg aggacgaaga ggagtggtgg ctctatggct cgacctggat
     1081 tctgctatcg cgcagcgagc aaatcatgaa ccgccccgac atccgcaatg cagccagcac
     1141 cgttaatacc aactccgtca aagtccccct ctggaccgac gacttcgcca gcctctttca
     1201 gatcttgcgc taaaggcgct gaactcatga gcacaactcc gcaatccccg ccaaacccgt
     1261 tcttgaattc tcaagccact cccttcgccg ggcggccgag cccattcgcc gtgcaaccgg
     1321 cgctgctggc attcctgatt tgcctggtgc tgggcctggc ggccgtgggc tggcgggcga
     1381 cagtccgtgc caaggacgcc cgcgcccgcg cgggtcttga ggcgctggcc cgaggcgccg
     1441 cggtggagct gcagttcagc caggtggtgt cggcagccga agtgctcggg gccttggccc
     1501 ggcagcacgg cggagcgatc cccgatttcc agaagctcgc ggcggatttg ctggtcgccc
     1561 ggcccgccct ggcttcgctc gaattgcagc cgggcggcat cgtcagtgac atcgtgccgc
     1621 gggccgctaa tgcgcgagcc gtcgggttca atgtgctgaa caatccgacg tatcgccccg
     1681 gcgccagcgc caccctccaa cggcgcagac tcacggtgac cgggcctttg gcactttaca
     1741 gcggcgaacc tggcatcgtg gcccgggtgc cgatcttcca gcggggccgt gatgggcgcg
     1801 aggccttctg gggctttgtg gctgtctcaa tgcgggtgtc cgaggcactg cgttgggcgc
     1861 aggtggatga ccttgcccgg catgggtaca actatgcgtt ttctgtggcg gcttccagac
     1921 gggagaaggg cttgaccctc gcggcccacg gcgcgctgtc ggcccaggcc gccgtgcaac
     1981 aacccgtccg cgcccaggat gtgacattcc gtctcatgct gcaaccgcga ggcggctggg
     2041 ttaacaagac aaagctggtg ctagaacttg tggccgtgct cgtcgcatcc ggcttggtct
     2101 gcctgctggt gaacctgctg gaaagccgcc gcgctgttga gctggcgctc acggatgcaa
     2161 accggcggct gaccggcgaa accgaggacc gaaagcaggc tcaggcggat tgccatgtcg
     2221 cgaggaatga agctgccgcc gcccaggccg agctcaaccg gacacgatcc gctctgaaga
     2281 gcagcaatga acaggaggtc cggctaagtg tgtccgtccg cacggcagaa gcggcggcgc
     2341 aggccatgca ggcagaacta gaccaggcac gcatggccct tcagcaagcg gagcaaagcg
     2401 ctgaacccag ccccgtcagc gtcagcgagg ggcaagacac ccacccggaa ctgatcgcgc
     2461 ctcccgaggc gcacgatgcc ccagcttcga gcagcgagcc cgctcccgag gccgtgccga
     2521 cgccagccac aactccggag ccggaatcgc cagccccctc aattcctgcg gaaccgccgc
     2581 ccccggctac ggcgctgccg gcggagccgc caacggagaa gaagcccgcc aaagtgccca
     2641 ggcgcaagaa ggcccgacgc aataaccaga tagatctctt tgcgccgcag cccacgccag
     2701 agccaacccc ggccatgccc cc
//
LOCUS       DATCGS010000474         8967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_536230, whole genome shotgun sequence.
ACCESSION   DATCGS010000474 DATCGS010000000
VERSION     DATCGS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536230"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..835
                     /locus_tag="VIL39_09745"
     CDS             41..835
                     /locus_tag="VIL39_09745"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5481750.1"
                     /translation="MSENLFDLTGKVAVVTGTSRGLGQYFGRALARAGADLVITSRDP
                     AKLAPFQAEIQALGRRALPLALDVRSFDSIRAMVEAAIAHYGKLDVLVNNAGCNVRKP
                     ALEVTWDDWNLVLDTNLRGTFFVSQAVARHMIPRKYGHIINIGSVTCVAGYAGLGPYG
                     ASRGGVKQLTMSLADDWGVHGITVNCLAPGWFKTEQNAVMYENQEWVEYLCDRIPLKR
                     PGQPQDLDGAVVFLASDASAYITGQTLLVDGGITTGATRALPKPGR"
     gene            complement(862..2403)
                     /locus_tag="VIL39_09750"
     CDS             complement(862..2403)
                     /locus_tag="VIL39_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HEY5481751.1"
                     /translation="MADIILTTLNAKYIHSAFGLRYLLANLGPLGAVACLVEFDIHQR
                     PVDIAEALLTRNPKIIGLGIYIWNVAPATEVIATLKRVRPDIIVILGGPEVSYESESQ
                     PIVQLADYVITGEADLKLAEVCRQLLSGEHPATKIIPAGLPEFSELALPYDLYDDQDV
                     AHRIIYVEASRGCPFSCEFCLSSLDIPVRQAPLPALLDHLQRLLDRGVRQFKFVDRTF
                     NLNLEAGRTILEFLLERYRPGHLFHFELVPDRLPDTLREVIARFPPGALQFEVGVQTF
                     NEEVAERITRRQDYARLEESLRFLRGQTGVHIHADLIAGLPGENLESFATGFDRLIAL
                     EPHEIQVGMLKRLRGTPIVRHDAEWQMVYNPHPPYELLQHRLIDFATMQKLRRFARYW
                     DLVGNSGNFVDTTPLIWSNVGQAPLSPFAGFMRWSEWLHGRVGRTDSIALVRLMELLF
                     EFLTGERSLDPKPTAAAMWRDYQRGGRHDKPAFLKDHLPADTLPASPRRTRSALPKRQ
                     ARHLA"
     gene            2619..5102
                     /locus_tag="VIL39_09755"
     CDS             2619..5102
                     /locus_tag="VIL39_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEY5481752.1"
                     /translation="MKNVIPSWHWRIALVVVSATLLGAGTQAVGQQVRALGIDVSAWQ
                     GNIPAANWATLKRATNAQVSGVYGDGKDFVMIRSSRGGTTGEDHRQGGYAAGNNTFYN
                     LSQRYDDLYFVQNINRATDAGLFAGSYHYGRADVLASTLNSDGTTTAGVANNGTDEAN
                     HFIQMAGAWMRPGYMLPTFDLEAGGGDQTSFSLDFSDRIYAVMGIRPIMYTGGNYTPY
                     LQSSIVDRFPTLWYPRYANQSSPDTIPVQTANPNDFPPVNYGPWDDFGNSQPWAFWQY
                     ASTARLNGINNKASNVDVDVAHGGIEFIKDILVPAFWMNDSDGQWTTLLNWNSGQTPQ
                     APVPGSGQVTPVGTQTLPTTRLPGINDTVILDRPGAAITVTLASGAQTIRKLYVRETL
                     NITGGSLSINYVPSWDSTTNGAQFSSPVTLSGSASLSVHTLQVDATRTFTLSGGTLTF
                     NTINLMPHSTTPAKMLMSGDVSLNALSNVTATIAKGTGSGSSGLIDLGGGNRSWNVAN
                     GSADVDLSVDVPVSNGALAKTGPGTMRLGVANAYSGGTTISAGILEGGVASSIPGNVT
                     NSAGTLKLDNASTLVSGATLALASSPSAGAVNLNFSGTQTVSALYFGTTRKAAGTWAA
                     SGATHNNAAFTGAGRLNVTTGPASSTAVTLTSGSSPSTYGDSLTFTATITGNSPGGTV
                     QFKVDGVAVGSPVTLVGGSASLVLSTLSVSGSPHQITAYYGGDDNNNPSNSSASLLAQ
                     VVTCSQTNALLSLVDNLDGTSTLTFMGTPQAEYYLLASPDVAAPMTSWAPVAGSTNTV
                     TNVNGLWQFTLTNTAPQQFYRSTAVVPCP"
     gene            complement(5038..5460)
                     /locus_tag="VIL39_09760"
     CDS             complement(5038..5460)
                     /locus_tag="VIL39_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEY5481753.1"
                     /translation="MFDNVRVERFVTNVPPYLTAQPQGQTNLAGASVTLSVTATGTAP
                     LCYRWQCDGNAYPPGANTFLACQAGDYSVVVSNVAGMVTSAVATVVFTNPAPALPGHF
                     DSISRLADGSVQLNARPITDKEQRPCCDRTAVAPCWLT"
     gene            complement(5506..5634)
                     /locus_tag="VIL39_09765"
     CDS             complement(5506..5634)
                     /locus_tag="VIL39_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481754.1"
                     /translation="MRTKLPAIVNKTGNMVEWFIDGLKIATISGATFASSNIFIGV"
     gene            complement(5655..6740)
                     /locus_tag="VIL39_09770"
     CDS             complement(5655..6740)
                     /locus_tag="VIL39_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481755.1"
                     /translation="MSENNPKYGWKTVRPVLAGVVLISLAIFIARTTPKPASPTQTSP
                     LAYPGSAAATNASQAAQSKWSTTRERVAGHLTAGRLSAQSILKYGRGEGEVGMVNEKE
                     QPPVGPESFAVQKDGSILVADVVNRRVAVYSSNGTYLRAIALPGIALGDVTADAQGRI
                     YVYDQVRHSLHQYDAQGRPQNELALSPADIDTRGYFHIAGNSVYFADAAVRDVLVATL
                     QDGVLTPADKALERTTDGIHGDSGRIYSMSLNKRQSLRLQVRDPATQSAARQLEVPLP
                     GIVSARYAGEDQAQQFYIQTERLDGTSIVLEVLVFSPTGEHLATTRMPENDYAVWTAK
                     LVAVGADGTIVQFLPQQNQAKLNLFAN"
     gene            7007..7681
                     /locus_tag="VIL39_09775"
     CDS             7007..7681
                     /locus_tag="VIL39_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481756.1"
                     /translation="MKNETKKTTDAQLLDLLKKVLPKATHDAQLAGKIYQAIELELKA
                     KARGTAFEKFCAKVELPDLEPKSLEDVKTQLAASFGQGDVTLKPDRKEKTVAVEVSLT
                     DGTQFSGEIKVNPNVGVEASEEQEITLKFVPFPVCLPGDKELVWLLGKRENLSAEEAG
                     IALTKVEEEFWASKTGQKLLRDRVERSFPEFIARAPAGMLNELGLKRHYKTPEPIKPL
                     RALKKS"
     gene            7760..8248
                     /locus_tag="VIL39_09780"
     CDS             7760..8248
                     /locus_tag="VIL39_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY5481757.1"
                     /translation="MLQHGPESGHQVAETELVLFKSSPIHGIGGFATTAIGKGTRIIE
                     YVGERISKGESLRRCEQNNEYIFTLNDEQNLDGNVAWNPARFINHSCAPNCEAELDNE
                     RIWIVVIRDMRAGEEITFNYGYDLEDYKDYPCRCGSPNCVGYMVAEVFFEHVLSRKAG
                     GP"
     gene            8356..>8967
                     /locus_tag="VIL39_09785"
     CDS             8356..>8967
                     /locus_tag="VIL39_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481758.1"
                     /translation="MRFESENERPSVARAGLVLNPRVRFLGQGARRLRPSVLLLVTAV
                     LLTTLGATVAASVPGGNRCAFIGISNFSSFSRSEGTVSNETIFTSPEIAAPIDWDELI
                     ASWNVPPGVHLKVEARAIYPNHPTRYYTMGLWSEDTAHFPRESVRRQRDQDGSVKMDT
                     LVLSNAVRKVQLRIIAGGAGRQGMLKFLGLSFCNSTVPATVLAP"
BASE COUNT         1804 a         2741 c         2770 g         1652 t
ORIGIN      
        1 tgatgcgtga tgcgtgaggg gagggagagc gaagacgact atgagcgaga acttatttga
       61 tctaaccggc aaggtggctg ttgtcactgg cacgagccgc gggcttggac agtacttcgg
      121 ccgggcgctg gcgcgggctg gcgctgacct ggtcatcacc agtcgcgacc cggcaaagct
      181 ggcgccgttc caggccgaga ttcaggccct cggccgccgg gcactgccgc tggcgctcga
      241 tgtgcgcagt ttcgacagca tccgggccat ggtcgaggcc gccatagcgc actacgggaa
      301 gctggatgtg ctggtcaaca acgccggctg caatgtgcgc aaaccggcgc tggaggtcac
      361 ttgggacgat tggaacctgg tcctggacac taacttgcgc ggcaccttct tcgtgtcaca
      421 agccgtggcg cgacacatga taccgaggaa atacggtcac atcatcaaca tcggctccgt
      481 cacctgcgtc gccggctatg ccggcctcgg gccctacggg gccagtcgcg gcggcgtcaa
      541 acagctcacc atgagcctgg ccgacgattg gggcgtccac ggcatcacgg tgaattgcct
      601 ggcgccaggc tggttcaaga ccgagcagaa cgccgtcatg tacgaaaacc aggaatgggt
      661 cgaatacctc tgcgaccgca tcccgctcaa gcgtcccggc cagccgcagg acctggacgg
      721 cgcggtggtg tttctggcct ccgacgccag cgcctacatc accggccaga ccctgctggt
      781 ggatggcggc attaccacgg gcgcaacccg cgccctgccg aagccgggac gctaagagag
      841 caagccggtc gcccgtccgg ttcacgccag atggcgggcc tggcgtttag gcagggccga
      901 ccgcgttcgc cggggagagg ctggcagcgt gtctgcgggc aaatggtctt tgaggaaggc
      961 aggcttgtcg tgccggcccc cgcgctggta atcccgccac atcgctgcag cggttggctt
     1021 cgggtcgagg ctccgctcgc ccgtcaggaa ctcgaacagt aattccatca gccggaccag
     1081 cgcaatgctg tcggtgcggc caacgcgccc gtgcagccat tcgctccacc gcatgaaccc
     1141 cgcgaaggga gagagtgggg cctgccctac attgctccag atcagcggcg tggtgtccac
     1201 gaagttgccg ctgttgccga cgaggtccca atagcgcgcg aagcgccgca gcttctgcat
     1261 cgtggcgaag tcaatcagcc gatgctgcaa cagttcgtac ggggggtgcg ggttatacac
     1321 catctgccat tccgcgtcgt gccggacgat cggcgtgcca cgcagccgct tgagcatgcc
     1381 tacctggatt tcgtgcggct ccagcgcaat caaccggtca aagcccgtgg caaagctctc
     1441 cagattctca cccggcaggc cggcaatcaa atccgcgtgg atatgaaccc ccgtctggcc
     1501 gcgcaggaac cggagactct cttccagccg cgcatagtcc tggcggcgcg tgatccgctc
     1561 cgccacctcc tcgttgaatg tttgcacccc cacctcgaac tgaagcgcac cgggcggaaa
     1621 ccgggcgatg acctcgcgca aggtgtccgg caaccggtcg ggcaccagct cgaagtggaa
     1681 gaggtggccg ggccggtagc gctcaagcag gaactccagt atcgtccggc cggcctcgag
     1741 gttgaggtta aaggtccgat ccacgaactt gaactgccgc acgccccggt cgagcaggcg
     1801 ctggaggtga tccagcaaag cgggcagggg agcttgccgg accgggatgt ccagcgatga
     1861 caggcagaac tcgcacgaga aggggcagcc acgggaggct tcgacgtaaa taatgcggtg
     1921 cgccacatcc tggtcgtcat aaagatcgta tggcagcgcc agttcggaaa actcgggcag
     1981 cccggcgggg atgatcttcg tggcgggatg ttcgccggag agcagttgcc ggcagacttc
     2041 agctaacttc aagtcggcct cccctgtaat gacgtagtct gccaattgca cgataggctg
     2101 gctctcgctc tcgtagctga cctccggccc gcccaggatg acgatgatgt ccggccgcac
     2161 ccgtttgagg gtcgcgatga cttccgtcgc gggcgcgacg ttccagatgt aaatgccgag
     2221 cccgataatc ttcgggttcc gcgtcagcag cgcttccgcg atgtccaccg ggcgctggtg
     2281 gatgtcgaac tctacgaggc aggcgacagc ccccagcggg ccgaggttgg ccagcaagta
     2341 gcgcagcccg aacgcggaat ggatgtactt cgcgttgagc gtagtcagaa taatatcggc
     2401 catcgtgagg tacaacttac cagcgcgccg aaggcgcggc aatgaagcag cgggcgcaga
     2461 cctggcgaga catggcgctt tacattctcg tttcgtgctg ctactccgtg gccagcatgt
     2521 gccagactgt acaccagtat tcccttgccg ccacggcatc aataagccgg ctgggcagca
     2581 gcaaccaatc gcctggcggt caatgcgccg aacgaggaat gaagaacgta atcccgagtt
     2641 ggcattggag aatcgcactg gtggtggtat cagcgacgct cttgggcgcg ggcacccaag
     2701 ccgtgggcca acaagtgcgc gccttgggaa ttgacgtatc cgcctggcaa gggaacatcc
     2761 ccgcagcgaa ttgggcgacg cttaagcggg ccaccaacgc gcaggtgagc ggggtctatg
     2821 gcgacggcaa agacttcgtc atgatccgct ccagtcgcgg cggcaccacc ggcgaagacc
     2881 accggcaagg tggttacgct gcggggaata acacgttcta taacctttcc cagcgatacg
     2941 acgatctcta cttcgtgcag aacatcaatc gcgccaccga cgcaggactg ttcgccggct
     3001 cctatcacta cggccgcgcg gacgtccttg ccagcacgct gaattcagac ggtaccacta
     3061 ccgccggggt ggccaacaat ggaacggatg aagctaatca cttcattcag atggccggcg
     3121 cgtggatgcg tcctggatac atgctgccga catttgactt ggaggcgggc ggtggagacc
     3181 agacttcctt ctccctcgat ttctcggatc ggatctatgc ggtaatgggc atccgcccga
     3241 taatgtacac gggtggcaac tatacgcctt accttcagtc atccatagta gataggtttc
     3301 cgaccctatg gtacccgcgc tatgcgaatc agagctcgcc tgatacgatc cccgttcaga
     3361 ccgccaaccc gaacgacttc cctccggtca attatggccc ttgggacgac tttggcaatt
     3421 cccaaccgtg ggcgttctgg cagtacgcca gcaccgcccg gttgaatggg attaacaaca
     3481 aggcgagtaa cgttgatgtg gacgtcgcgc atggtgggat cgaattcatt aaggatattc
     3541 ttgtcccggc gttttggatg aacgacagcg acgggcagtg gacaacccta ttgaactgga
     3601 acagcggaca gacgccgcaa gctccggtgc cggggtcggg gcaggtgacg cccgtgggga
     3661 cgcagacgct tcccaccacg cgcctgccgg gcatcaatga caccgtcatc ctcgaccgtc
     3721 ctggcgccgc tattaccgtc acgctcgcct ccggcgcgca gacaattcgc aagctctatg
     3781 tgcgcgagac cttgaacatc accggcggat cactgagcat caactacgtc ccatcatggg
     3841 attccacaac gaacggggcc cagttctcga gcccggtcac gctcagcggc agcgccagcc
     3901 tcagcgtcca cacgctccag gtggatgcca cccgcacgtt cacgctcagc ggcggcacgc
     3961 tcaccttcaa caccatcaac ctcatgcccc acagcaccac gccggcgaag atgctgatga
     4021 gcggagacgt gagtctcaat gccctctcca atgttaccgc caccatcgcc aagggcacgg
     4081 gctcgggcag ctccggcctg attgatctcg gcggcgggaa ccgctcttgg aatgttgcca
     4141 acggctcagc cgacgtggac ctgtccgttg atgtccctgt ttccaacggt gctctggcca
     4201 agaccggccc gggcacgatg cgcctcggcg tcgccaatgc gtacagcggc ggcacgacca
     4261 tcagcgccgg catcctcgaa ggcggtgtcg ccagttcgat cccgggcaac gtcacgaata
     4321 gtgccggcac gctcaagctt gataatgcct ctaccctggt ttccggcgct accctcgcct
     4381 tggccagttc acccagcgcc ggcgcggtaa acctcaactt ctccggcact cagaccgtca
     4441 gcgccctcta tttcggcacg acccgaaaag ccgccggcac atgggctgcc tccggcgcca
     4501 cccacaacaa cgccgctttt accggcgccg gcaggctcaa tgtcactacc gggcctgcct
     4561 cgagcaccgc cgtcacgctg acctccggct ccagtccctc cacttacggt gattccctga
     4621 ccttcactgc caccatcacg gggaattcac ctggcggcac ggtccaattc aaagtggacg
     4681 gcgtagccgt gggcagcccc gtcaccctgg tcggtggttc ggcatccctg gtcctcagca
     4741 ccctctctgt ctccggcagt cctcatcaga ttaccgccta ctacggtggc gatgataaca
     4801 acaaccccag caacagctcc gccagcctgt tggcgcaggt cgtcacctgc agccagacta
     4861 acgcgctgtt gagtctggtg gacaatctcg atgggacgtc cacactgacg tttatgggca
     4921 cgccgcaggc ggagtactac ttgttagcaa gcccggatgt ggcagcgccg atgaccagtt
     4981 gggcgccggt agcaggcagc accaacacgg tcaccaatgt caatggcttg tggcaattca
     5041 cgttaaccaa cacggcgcca cagcagttct atcgcagcac ggccgttgtt ccttgtccgt
     5101 aatgggtctt gcgttcaact gcacggagcc atctgccagc cggctgatcg aatcgaagtg
     5161 gcccggcagg gccggcgccg ggttggtgaa gaccaccgtg gccaccgcgc tcgtgaccat
     5221 gccggccacg ttgctcacga cgaccgaata gtcgcccgcc tggcaggcca ggaaggtgtt
     5281 ggcgcccggg gggtaagcgt ttccatcaca ctgccatcgg tagcacaacg gtgccgtgcc
     5341 ggtcgccgtg accgacaagg tgacgctggc gcctgccagg tttgtctggc cttgcggctg
     5401 ggcggtgagg taggggggca cgttcgtcac gaaccgctcc acccgcacgt tgtcgaacac
     5461 cacgaagctc aggttggtgt tgtccgagag cgaactgaac gaatcctaga ccccgatgaa
     5521 gatgttgctg gacgcgaagg tcgctccgct gatggtggcg atcttcaggc cgtcaatgaa
     5581 ccactcgacc atgttgccgg tcttgttcac gatcgccggc aacttggttc tcatccaggt
     5641 caatcgtctt gtgactaatt cgcaaacagg ttcagcttgg cctggttctg ctggggcagg
     5701 aactgcacga tcgttccatc agctcccacg gccacgagct tggcggtcca gacggcataa
     5761 tcgttctccg gcatccgcgt ggtggccaaa tgctcgccgg tcgggctgaa gacaagcacc
     5821 tccagcacga tgctggtgcc gtccaaccgc tcggtctgga tataaaactg ctgggcctga
     5881 tcctcgcccg catatcgcgc tgagacaatg cccggtaacg gcacctcaag ctggcgagcc
     5941 gctgactgag tggctgggtc gcgcacttga agccgcagcg actgtctctt attcaaggac
     6001 atcgagtaaa tgcggccact gtcgccgtgg atgccatccg tggtccgctc caatgctttg
     6061 tcggctggcg tgagcacccc atcttgcaag gtcgcgacca ggacatctcg gacggcagca
     6121 tcggcgaaat agacggagtt gccggcgatg tggaagtagc cccgggtgtc tatatcggcg
     6181 gggctgaggg caagctcgtt ttgcggcctg ccctgcgcgt catattgatg cagagaatgg
     6241 cgcacctggt cgtaaacata aatcctgcct tgcgcgtcgg cagtcacatc cccgagggcg
     6301 attcccggga gcgcgatggc gcggaggtag gtcccgttgc tggaatagac cgcgacccgc
     6361 cgattcacca catccgccac caggatgctt ccgtccttct gtacggcgaa cgattccggg
     6421 ccgaccgggg gctgctcctt ctcgttaacc atgccgacct cgccctcccc gcgaccgtat
     6481 ttcaggatgc tttgggcgct caaccggccg gccgtcaaat ggccggccac ccgctcgcgt
     6541 gtcgtggacc atttgctctg agccgcttgc gatgcgttcg tcgccgctgc tgagcccggg
     6601 taggcgagcg gggaagtttg ggtcgggctg gcgggcttcg gagttgtccg ggcgatgaag
     6661 atggccaggg aaatcaacac cacccccgcc agaacggggc gcaccgtctt ccacccgtac
     6721 tttggattgt tctcgctcat cgccgcaaat cgaggctatt gcaggacaaa ctgccccctg
     6781 aaggaagacg ccattggata gggccgcttc gagtcgccga ccgcaccgag tattgcttgt
     6841 gccgcacgat tccgagtccg ttggtaaacc ggggttgtcg ctcgctagaa gcgttgcccg
     6901 atcctgaccg gagatgatca taagggatgg ggccgggaaa tcaagccaag accaattaca
     6961 ttgctctccg ggccgggatt tggcacaaga ctgggccaaa tctaaaatga agaacgaaac
     7021 aaagaaaacg accgacgccc aattgctcga tttgctgaag aaagtgctgc ctaaagccac
     7081 gcacgatgcc cagttggctg gcaagattta tcaggccatc gaactggaac tcaaagccaa
     7141 agcccgcggc acggccttcg agaagttctg cgctaaagtg gagttgcccg acctggagcc
     7201 caagagcctg gaggacgtaa agacacagtt ggccgcatcc ttcggccaag gcgatgtgac
     7261 cctgaagcca gatcgcaaag agaagactgt ggccgtggaa gtctccttga ccgacggcac
     7321 tcagttcagc ggggaaatca aggtgaaccc gaacgtcggg gtggaggcca gcgaggagca
     7381 ggaaatcacg ctcaaattcg tgccgtttcc ggtctgcctg cccggtgaca aggaactggt
     7441 ctggctgctg ggcaagcgcg agaacctgtc agccgaggag gccggcatag cgttaacgaa
     7501 ggtggaggag gagttttggg catcgaaaac cggccaaaag ctattgcgcg accgggtcga
     7561 gcgcagcttc ccggagttca tcgcccgagc cccggctggt atgttgaacg aactgggctt
     7621 gaagcggcac tacaagactc cggaaccgat caagccgctg cgagcactga agaaaagcta
     7681 gcagaacggg ctgccgaact tcccttttta tgagaagcgc gagaacaagc gagaatcgtg
     7741 agtaagcatg gacggggggg tgttacagca tggacctgaa agcggccacc aggtggccga
     7801 gacggagctg gttctattca agagttctcc aattcacggg attggtggat ttgcgacgac
     7861 ggctattggc aaaggcacac gcatcatcga gtacgtaggc gagaggatca gcaagggtga
     7921 gtcgttgcgc cggtgtgagc agaacaatga atatatcttc acactgaacg acgagcagaa
     7981 cctcgacggc aatgtggcgt ggaacccggc gcggtttatc aaccatagct gcgcacccaa
     8041 ttgcgaagcc gagctggaca acgagcgtat ttggattgtc gttattcgcg acatgcgggc
     8101 aggggaggag atcacgttta actacggcta cgatctggag gattacaagg attacccgtg
     8161 ccgctgcggc tcgccgaatt gcgtcggcta catggtggcc gaggtattct tcgagcacgt
     8221 gctcagccgc aaagcaggag ggccttgatt cacgcgcagc ggcgccaggg tcgtgccgct
     8281 cggcgagtgt ggaataaata gatagacaag aatagggctt gccagattgc tcgcggtttg
     8341 gagcctatgg cggatatgcg ctttgaatct gagaatgaaa gaccgtcagt tgcccgcgcc
     8401 ggcttggttc tgaaccccag ggttcggttc ctggggcaag gggcgaggcg gcttcgccct
     8461 tccgtcctcc tgctggtcac ggcagtgctg ctgaccactt tgggcgcgac cgtcgcggcg
     8521 agcgtcccgg gcggcaatcg ctgcgcgttc attgggatca gcaacttctc gagcttttca
     8581 aggagcgagg gcaccgtgag taacgagacg atcttcacct cgcctgaaat agcggccccg
     8641 attgactggg atgaattgat cgcttcctgg aacgtgccgc ctggcgtgca tctgaaagtg
     8701 gaagcccgcg cgatctatcc caatcacccg acgcggtatt acacgatggg cttatggtca
     8761 gaggatacgg cgcactttcc gcgggagagc gtgcggcggc aaagggatca ggacggttcg
     8821 gtgaagatgg acacactggt gctgagcaac gctgtccgca aggtgcagct tcggatcatc
     8881 gcggggggag ccggcagaca agggatgctc aagttcctcg gcctttcgtt ctgcaacagc
     8941 accgtgccgg caaccgttct ggcgccg
//
LOCUS       DATCGS010000475         6064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_585121, whole genome shotgun sequence.
ACCESSION   DATCGS010000475 DATCGS010000000
VERSION     DATCGS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585121"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3053)
                     /locus_tag="VIL39_09790"
     CDS             complement(<1..3053)
                     /locus_tag="VIL39_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY5481759.1"
                     /translation="MKFSHFFIRRPIFASVLSLLIVLLGAIALFTLPVAQYPEITPPT
                     IFVIASYPGASADTVANTVATPLEEQINGVENMLYMSAQCSSDGQMRLAITFKVGTNP
                     DTAQVLVQNRVDGALPRMPEEVRTLGVITRKRAPSIAMLVSLVSPSGKYDPVYLSNYA
                     YLHVRDVLARLPGVGDSTLFGARDYCMRIWIDPNKASARSLTASDIVAAIRRQNVEVA
                     AGLFGQSPQPPDNLFQLAVVTKGRLVTPEEFGDIVLKAQAEGQITRLRDVARIELAAN
                     DYSIDSRLNGRPAAAIALFQQPGSNAIETANAIEAAMKELKKDFPEGLDYRIAYDTSA
                     FIRESIRAVVETLIIAILLVIFVVVLFLQNWRASLIPLVSVPVSLIGTFAAMTAFGFS
                     INNLSLFGLVLAIGIVVDDAIVVVENVERHIAEGLSPADATHKAMDEVSGAVIAIALV
                     LSAVFIPTAFVSGISGRFYQQFALTIAISTLISAFTSLTLSPALCALLLQPHHASKDF
                     IGRVMHNSVDWLFRAFNRVFEGGRAKYVRALGGVLRHCGVTLVLYAALLGLTWFGFHK
                     VPTGFIPSQDKGNIFCFLQLPDGASLQRTELVSQRVVQLLTNTPGIAAVSEFAGLSLV
                     ALGNSANASSMFVRLSPFEERVKAGLTADVIIANLRKTIAQANIQEAYIVVAGTPPVD
                     GLGTLGGFKLQVEDRGNVGYPALQAATFQLVGAAMAQTNKITQALSTFRASVPQVYLD
                     VDRAKAESMHVPLDAVWDTLSIYLGSLYVNDFTLFGRPYHVTAQADAPFRAKPTDVKN
                     LKTRNASGEMVPLGTLVKVKDINAPVLAGHFNMFPTAEILGGTAPGVSSGDAIRLMTD
                     LADKVLPQGIRIEWTELSLLQILAGNTAAYIFPLCVLMMFLVLAAQYESWSLPLAIIL
                     IVPMCLLFAIFGVWLRGLDNNLFTQIGLVVLMGLACKNAILIVEFAKQLQDAGRSRTE
                     AAIESSRLRLRPILMTSFAFTFGVIPLMLSHGAGAEMRVSIGTAV"
     gene            complement(3056..4294)
                     /locus_tag="VIL39_09795"
     CDS             complement(3056..4294)
                     /locus_tag="VIL39_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5481760.1"
                     /translation="MSLSSTSTPRLAEQCESRRLKKGFLPWLLPAICVAALTACSPPK
                     PASLPPSKVTVSHPQLATVTNWDEYPGHLEAVEMVEIRPRVSGYIDSIHFQDGTEVKA
                     GDLLFVIDPRPYQAELDHVQAQRQQAETHLGLLRNDLQRAEGLRGTKAISEEEYDSRS
                     NAVREAEAALVAAKANESTARINLDYTQIKAPISGKIGRRLVTAGNFVQLQGNGGSAT
                     VLATIVSVNPIYGYFDVEESAFLKYRSNAKAGEAKGDGLTCELALVNEEGFAHQGRLD
                     FFDNQVNPQTGTIRLRAVFVNDDRALLPGMYANVRVLAGLPEQTFVVPDVAVGSDQGY
                     KYVYTVNAEDVVKKQDITTGRAHGALRAVVKGLTAEDRVIVNGLMMIRPGAKVVVQSS
                     EAKAESDKATAATEKQQAHH"
     gene            complement(4320..4643)
                     /locus_tag="VIL39_09800"
     CDS             complement(4320..4643)
                     /locus_tag="VIL39_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEY5481761.1"
                     /translation="MNPVFSKSDPAASLCGNSAHRPLHLVDFFCRAPQAKSVFLIGDF
                     NEWQPKAHPMTRMPDGGWVIRMELPHGHHQYLFLMDGKPTLDPNAMGQVHNERNETVS
                     LIAIS"
     gene            complement(4657..5316)
                     /locus_tag="VIL39_09805"
     CDS             complement(4657..5316)
                     /locus_tag="VIL39_09805"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5481762.1"
                     /translation="MPKQSEQDRQTRILDAAENAIAELGFAGASLRHIVLEAQVNLAT
                     VYYYFGSKGGLMEAVLKRRFGPLRQEHLALLQQFEQAAGGRPLKVEKILEAMLLPPLR
                     LMATASDKHHAVTRLIGRIVAEPNPETQEMVRSQRATVRAAFLKALQRRLPRAPVPAL
                     VWRLEFVWGALAFILCNPRRIEIETHGACNPADTANLLAEMVRCFSPGFQTLAREKRK
                     L"
     gene            5441..5971
                     /locus_tag="VIL39_09810"
     CDS             5441..5971
                     /locus_tag="VIL39_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5481763.1"
                     /translation="MTTTRDDNLGFILGRRSIRVYSPGEVSEPAVTRLLEAAMAAPSA
                     MTKDPWRFVVVRDKQTLSKLAALHPGAAMLSSAAMAIVVCGDLDAAFERHISYLLQDC
                     SAATENLLLAAHGQGLGACWVGLHPGEPLIKRVKELLSLPASFVPVAVVSLGQPGEQP
                     APCTRYNPAHVRAEKW"
BASE COUNT         1155 a         1864 c         1923 g         1122 t
ORIGIN      
        1 aacactgcgg tgccgatgga cacgcgcatt tccgccccgg cgccgtggga gagcatcagc
       61 gggatgacac cgaaggtgaa cgcaaaagag gtcatcagga tggggcgcag ccgcaagcgc
      121 gaggactcga tggcagcctc ggtgcggctt cgtccggcgt cctgcaactg cttggcgaac
      181 tcgacaatga ggatggcgtt cttgcaggcc aaccccatca gcaccaccag cccgatctgg
      241 gtgaagaggt tgttgtccag gccccgcagc caaacaccga agatcgcaaa gagcaggcac
      301 atcggcacga tgaggatgat cgccagcggc agcgaccagc tctcgtattg cgccgccaat
      361 accaggaaca tcatcagcac gcagagcggg aagatgtagg cggcggtgtt gccggccaga
      421 atttgcaaca ggctcaactc cgtccactcg atccttatgc cctgcggcag gactttgtcg
      481 gccaggtccg tcatgagccg gatggcgtca cctgagctga cgcccggcgc ggtgccgccg
      541 aggatttcgg cggtgggaaa catattgaaa tggccggcca gcacgggggc gttgatgtcc
      601 ttcaccttta ccaaggtgcc cagcggcacc atttccccgc tggcgttgcg cgtcttgagg
      661 ttcttcacgt cagtcggctt ggcgcgaaac ggcgcgtcgg cctgcgccgt gacgtggtac
      721 ggccggccaa aaagggtgaa gtcattgacg tacaaggagc cgagatagat tgacagggtg
      781 tcccaaaccg cgtcgagcgg cacgtgcatg gattcggcct tggcacggtc cacgtccagg
      841 tacacctgcg gcacgctggc gcggaaggtg gagagcgcct gggtgatctt gttggtctgg
      901 gccatggcgg cgcctaccaa ctggaaggtg gccgcctgca gtgccgggta accgacattg
      961 ccgcggtctt cgacctggag cttgaagccg cccaacgttc ccagcccgtc caccgggggc
     1021 gtgccggcga ccacgatgta ggcctcctga atgttggcct gggctatggt ttttcgcagg
     1081 ttcgcgatga tgacgtccgc cgtcaggccc gccttcactc gttcctcgaa aggactcaag
     1141 cgaacgaaca tgctcgacgc gttggcgcta tttcccaggg caaccagcga caacccggcg
     1201 aattccgaca cggcggcaat gccgggcgta ttagtcagca actgcacgac tcgctggctg
     1261 accagctccg tgcgctggag ggatgcgccg tccggaagct ggagaaagca gaagatattg
     1321 cctttgtcct gggacggaat gaagcccgtg ggcaccttgt ggaagccgaa ccaggtcagg
     1381 cctagcaatg ccgcatagag cacgagcgtg acgccgcaat gccgcagcac cccgcccagc
     1441 gcccggacat acttcgcgcg gccaccctcg aagacgcggt tgaaagcccg gaacagccaa
     1501 tccaccgagt tgtgcattac gcggccgatg aagtccttgg aggcgtggtg gggctggagc
     1561 agcagcgcgc acagggccgg gctgagtgtc agcgaggtga acgccgagat cagcgttgat
     1621 atcgcgatgg tcagcgcaaa ctgctggtag aatctgccgc tgatgccgct gacgaaggcg
     1681 gtgggaatga ataccgcgct cagcaccagc gcaatggcga tcaccgcgcc gctgacctcg
     1741 tccatcgcct tgtgggtcgc gtccgccggc gagagcccct ccgcgatatg ccgttccaca
     1801 ttctcgacca ccacgatggc gtcatccacc acaatcccga tcgccagcac cagcccgaag
     1861 agcgacaggt tgttaatcga aaacccaaag gccgtcatcg ccgcgaacgt gccgatcagc
     1921 gacaccggga ctgacaccag cggtatcagc gacgcccgcc aattctgcag gaacagcact
     1981 acgacaaaaa tcacgagcag gattgcgatt atcagggttt cgaccacggc ccggatggac
     2041 tcccggatga aggccgaggt gtcgtaagcg atccggtaat ccagcccttc ggggaaatct
     2101 ttcttcagct ccttcatcgc cgcctcgatg gcgttggccg tctcgatggc gtttgatccc
     2161 ggctgctgga acaaggcaat cgccgccgcc ggtcgcccgt tgagccggct gtcaatgctg
     2221 taatcgttgg ccgccaactc gatgcgcgcg acatcccgca accgagtgat ctggccctcg
     2281 gcttgcgcct tgagcacgat gtccccaaac tcttccggtg ttaccagccg gcctttggtc
     2341 acgaccgcca gttggaacag gttgtcgggc ggctgcggcg actggccgaa cagccccgcc
     2401 gccacctcca cattctgccg gcggatcgcc gccacgatgt cgctcgctgt cagactgcgc
     2461 gcggaggcct tgttcggatc aatccagatg cgcatgcagt aatcccgcgc gccaaagagg
     2521 gtcgagtccc ccacccccgg cagccgggcc agaacgtccc gcacgtgcaa ataggcatag
     2581 ttgctcagat ataccgggtc atacttgccc gacggcgaaa ccaggctcac cagcatggcg
     2641 atgctgggcg cgcgtttgcg tgtgattacg cccagcgtgc gcacttcctc cggcatgcgg
     2701 ggcaaggcgc cgtccacgcg gttctgcacc agcacctggg cggtatccgg gttcgtgcct
     2761 accttgaacg ttattgccag ccgcatctgg ccgtcgctgc tgcattgcgc cgacatgtaa
     2821 agcatgtttt ccaccccgtt gatttgctct tccagggggg tggccaccgt gttagcgacg
     2881 gtgtcggcgc tggctccagg atagctggcg atgacgaaga tggtcggggg cgtgatctct
     2941 ggatactggg caaccggcag cgtaaacagc gcgattgccc ccaacagcac gatcaggagg
     3001 gacaggaccg acgcaaagat cggtcggcga atgaagaaat gggagaattt cacggttagt
     3061 gatgggcttg ctgcttttcg gttgccgcgg tcgccttgtc gctttcggct ttggcctcgg
     3121 aactctggac caccaccttc gcgcctggcc ggatcatcat caggccgttg acaatcaccc
     3181 ggtcttccgc tgtcagcccc ttgaccacgg cgcgcagcgc accgtgagcc cggcccgtcg
     3241 tgatgtcctg cttcttgacg acgtcttcgg cgttgacggt atagacatac ttgtagccct
     3301 ggtcggaacc cacggccacg tccgggacaa cgaacgtctg ctcgggcagc ccggcgagca
     3361 cgcgcacatt ggcgtacatg cccggcagca gggcgcggtc gtcgttaaca aagacggcac
     3421 gcagccggat ggtgccggtc tgcgggttaa cctggttatc gaagaagtcg agccggccct
     3481 ggtgcgcgaa gccttcttcg ttcaccaacg ccaattcgca agtgaggccg tcgcccttgg
     3541 cctcccccgc cttcgcattg ctccggtatt tgaggaacgc gctttcctcc acgtcgaagt
     3601 agccatagat aggattaacc gagacgatgg tggccagcac ggtggccgat ccgccattgc
     3661 cttgaagctg gacgaagttg ccggcggtca cgagccggcg cccgatcttg ccgctgatcg
     3721 gagccttgat ttgcgtgtag tcgagattaa tgcgggccgt gctctcgttg gccttggcgg
     3781 cgacgagagc ggcttcggct tcgcggactg cgttgctgcg gctgtcgtat tcctcctcgg
     3841 atattgcctt ggtgccccgg aggccttcgg cgcgctgaag gtcgttacgc aacagtccca
     3901 gatgggtctc ggcctgctgg cgttgggcct gcacatggtc tagttcggcc tgataaggcc
     3961 ggggatcaat cacgaacagc aagtcgccgg cctttacctc ggtgccgtcc tggaaatgga
     4021 tggagtcaat gtaaccggag acacgcggtc ggatttcgac catctccacc gcctccaggt
     4081 ggccggggta ttcgtcccag ttggtgaccg tcgcgagctg aggatggctg acggtaacct
     4141 tcgagggcgg cagcgaggcg ggcttgggcg gcgagcaagc cgtcagcgca gcgacgcaaa
     4201 tggccggaag cagccacggg agaaaccctt tcttgaggcg ccgactctcg cactgctcgg
     4261 cgagacgcgg ggttgaggta gatgataaac tcataggcat tagatatggc gttcggaagt
     4321 taactgattg cgatcaagga aaccgtctca ttgcgttcat tatgcacctg ccccattgcg
     4381 ttcggatcga gggtcggctt cccgtccatg aggaacagat actgatggtg gccatgggga
     4441 agctccatcc gaatgaccca gccgccatcc ggcatccgtg tcatcggatg ggccttaggc
     4501 tgccactcgt taaagtcacc aatgagaaag acacttttgg cctgcggcgc gcggcagaag
     4561 aagtccacca ggtggagggg gcggtgcgca gagttcccgc aaagagaggc tgctgggtct
     4621 gacttggaaa acacagggtt cataacgcgg tccagctcag agcttccgct tttcccgggc
     4681 cagggtctgg aagcccggcg agaagcaccg gaccatttcc gccagcaaat tggccgtgtc
     4741 ggcagggttg caggccccat gcgtctcgat ctcgatcctg cgcgggttgc acagaatgaa
     4801 cgcgagcgcg ccccagacga attccaggcg ccagaccagg gcgggcacgg gggcccgggg
     4861 cagtctgcgc tgcaacgctt tgagaaaagc ggcgcggacc gtggcccgtt gggagcgcac
     4921 catctcctga gtctccgggt ttggctcggc cacgattcgg ccgatcaggc gggtgaccgc
     4981 gtgatgcttg tcagacgccg tggccatcag ccgcaacggt ggcagcagca ttgcttccag
     5041 tatcttctcc acctttagcg ggcgacctcc ggctgcctgc tcgaactgtt gcagcagcgc
     5101 caggtgctcc tgccggagcg ggccgaagcg ccgcttgagc actgcctcca tcaggccgcc
     5161 tttggagccg aaataataat aaaccgtggc caggttcact tgggcctcga gcacaatgtg
     5221 tcgcagcgac gcgccggcaa accccagttc cgcgatggcg ttctccgcgg catccaggat
     5281 gcgggtctga cggtcttgtt cagattgctt aggcatttaa ctaaacgtgc gttttaaacg
     5341 aacgttaaaa tcatacagca tccttgccgg gggtcaaaca ggatttgaaa ctccacgcgg
     5401 aaaccactaa acagatgggc tctgattcaa cgatacaact atgactacga ccagagacga
     5461 taatttgggc tttattcttg gccggcgcag catccgggtc tattcgcccg gcgaggtgag
     5521 cgagccggcg gtgaccaggc tgctggaagc ggcgatggcc gccccctcgg ccatgaccaa
     5581 ggacccctgg cggttcgtgg tcgtccggga caagcaaaca ctctcgaagc tggcggcgct
     5641 ccatccgggc gcggcgatgc tctcttccgc ggcgatggcg atcgtggtgt gcggcgacct
     5701 ggacgcggcc ttcgagcggc acatcagcta cctgctgcag gactgttcgg cggccaccga
     5761 gaacctgctg ctggccgcgc acgggcaagg tctgggtgcc tgctgggtgg gactccaccc
     5821 cggcgaacct ctgatcaagc gggtgaaaga actgctctcc ctgcccgctt cattcgttcc
     5881 ggtggcggtg gtatcgcttg gccagccggg cgaacagcca gccccctgca ctcgctacaa
     5941 cccggcccac gtccgcgccg agaagtggtg aactgggagg gcgagcgtca ctgctattaa
     6001 aaaagtaatg ggtcagtgac ggcggagacg acggtatcgg agtgcgcccg tcctcgggcg
     6061 cagc
//
LOCUS       DATCGS010000476         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_684360, whole genome shotgun sequence.
ACCESSION   DATCGS010000476 DATCGS010000000
VERSION     DATCGS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684360"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VIL39_09815"
     CDS             complement(<1..1038)
                     /locus_tag="VIL39_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5481764.1"
                     /translation="MQAVINYLKEHQSRAIAELCEYVRFPSVSAQPQHRRDLRACAEW
                     VVKHCRAIGLEARLCSTKGHPIVVAKTPRAKSPGARRPHFVVYGHYDVQPPEPLELWK
                     SPPFEPRVFKGALFGRGASDNKGQNLAHIKAVEAYLKTGTKLPCDITFVIEGEEEVGS
                     SSLAAFLREHRAELACDAIVISDTGMPTPKHPALTYALRGIASFQVTLFGPTRDLHSG
                     IFGGTVDNPAMALCQLLGKLRDKDGRVTVPGFYQGVRPLSAYERKQFARLPFKATEYQ
                     KFLGVPKLFGERGFTPLEQRSARPTFEINGLTSGYQGEGSKTIVPAWARAKITVRLVP
                     NQNPTEVVKRIR"
     gene            1241..2443
                     /locus_tag="VIL39_09820"
     CDS             1241..2443
                     /locus_tag="VIL39_09820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY5481765.1"
                     /translation="MQSLPTVLLKPGEADRILAGHPWLYHGSVLRLTQPAADGELVQI
                     KDHRQRFLGTGFYNSRSKINVRVLSPERIEPNQAFFEERIRAALAVRQRHMPAATSFR
                     VVNAESDFLSGLIVDKYEDVLVVQISSLGMDQRKPVIVAALQAIFSPRAIVERGELSS
                     RKFEGMADASGVLAGTLEGPVPINLNGLRFEVDVFEGHKTGLYLDQQVNYQRVAELAR
                     GGQVLDCFSFLGGFGLHAARAGAAHVHLLDQSAEAIAAATRNATANGLAEKCSAEAIN
                     VFDWLKAQTLTQPHEKLVPRWDLVVLDPPSFTRNRASVPDALRGYKEIHLRALKILKS
                     GGTLATFCCSHHVDASLFQDTVLSAAFDARRILRRIATYAQSPDHPIIPMIPETEYLK
                     GFAFEVVR"
BASE COUNT          465 a          771 c          791 g          488 t
ORIGIN      
        1 ccgaatccgc tttacaacct cggtcgggtt ctgattgggc accagccgga cggtgatctt
       61 ggcgcgcgcc caggccggca cgatcgtctt gctcccttcg ccctggtaac cgctcgttag
      121 cccgttgatc tcgaaggtcg gacgggcaga gcgttgttca agcggtgtga aaccgcgctc
      181 gccaaacaac ttcggcacgc cgagaaactt ctggtattcg gtggctttga agggcagccg
      241 cgcaaattgc ttgcgctcat aggcggacag aggccgcacc ccttgataaa agccgggcac
      301 ggtcacacgg ccatccttgt cgcgcagttt ccccagcagt tggcacagcg ccatggccgg
      361 gttgtccact gtcccgccga aaatgcccga gtgcagatcg cgcgtcgggc cgaacagcgt
      421 cacctggaag ctggcgattc cccgcaacgc gtaggtcagc gctgggtgtt tgggcgtcgg
      481 catgcccgtg tcagagatga cgattgcatc gcaggccagt tccgcccggt gctcccgcag
      541 aaaggcggcg aggctgctgc tgcccacctc ttcttcgcct tcgatgacga aggtaatgtc
      601 acacggcagc ttggttccgg tcttgagata ggcctccacc gccttgatgt gcgccaggtt
      661 ctggcccttg ttgtcgcttg cgccgcggcc gaagagcgct cctttgaaga cgcgcggctc
      721 gaacggcggc gatttccaga gctccagcgg ctcgggcggc tgcacatcgt aatgtccata
      781 aaccacgaaa tgcggccggc gggcgcctgg cgatttggcg cgcggggtct tcgcgacgac
      841 aatcgggtgg cccttggtgg agcaaagtcg cgcctccaga ccgatggccc ggcaatgttt
      901 cacgacccac tccgcacagg cgcgcaaatc ccggcggtgt tgcggctgag cggaaacgct
      961 ggggaaccgc acatactcgc agagttcggc gatagcgcgc gactggtgtt ccttcaggta
     1021 gttaatgaca gcttgcatcc ctgattttgt agcagagaaa ggcccgaaag gaaagggaag
     1081 ttcacgaggg caaggatgtc atttggtgct tttccctgcc gggcttcggc cctatgctgc
     1141 ggtgtctgga gttcggcctt gaggctttca gatttgccta atggctgcgc gcgtcagacc
     1201 acgcaaaggc ggaagccaga ctgaacttca aactaaaact atgcaatcat tgccgactgt
     1261 tctgctcaag ccaggcgagg ccgaccgcat cctggctggc catccgtggc tttatcatgg
     1321 ttccgtgctc cggcttacgc agcctgcggc cgatggcgaa ctggtgcaga tcaaagacca
     1381 tcgccagcgc tttctgggca cgggcttcta taattcgagg tcgaaaatca acgtgcgcgt
     1441 tctctccccg gagcggatcg agccgaacca ggcgttcttc gaggagcgga ttcgggcggc
     1501 tttggccgtc aggcaaaggc acatgccagc cgccacctcg ttccgcgtcg tcaatgccga
     1561 gagcgatttt ctcagcggcc tcatcgtgga caaatacgag gacgtgctgg tggtgcagat
     1621 ttcctcactc ggcatggacc agcgcaagcc ggtgatagtt gccgcgctgc aggcgatctt
     1681 ctcgccgcgg gcaatcgtgg agcggggtga gctttcctcg cgcaagtttg aaggaatggc
     1741 ggatgcgagc ggcgttttgg cggggacgct tgaaggtccg gtgccgatca acctgaacgg
     1801 cctgcggttc gaagttgatg tgttcgaggg ccacaagacg ggtttgtatc tcgaccagca
     1861 ggtcaattac cagcgcgtgg ccgaactggc caggggcggt caggtgctgg attgcttcag
     1921 cttcctgggc ggctttgggc tgcacgcggc ccgcgccggg gccgcgcacg tgcacctgct
     1981 cgaccagagc gcggaggcta tcgctgccgc cacacgcaac gcgaccgcta atggcctcgc
     2041 cgagaaatgc tccgccgagg caatcaacgt cttcgactgg ctcaaggcgc aaaccctgac
     2101 ccagccccac gagaagctcg tcccccgttg ggatttggtc gtgctcgatc cgccgtcctt
     2161 tacccgaaac cgcgcttcgg tgcccgacgc gttgcgcggt tacaaagaga tccacctgcg
     2221 cgccctcaag attctcaaga gtggtgggac gctggcgacc ttctgctgtt cgcaccacgt
     2281 ggacgccagc ctattccagg acacagtcct ttccgccgcg ttcgatgccc ggcgaattct
     2341 aaggcgcatc gccacctacg cgcagtcccc ggaccacccg attatcccga tgatccccga
     2401 gacggagtac ctgaaggggt ttgcgttcga ggtggtgagg tagcgggtgg gagaaacccc
     2461 gaaacccgag ggccgaaacc cgaaggaagg ccgaaatccg aaatccgaag cggcc
//
LOCUS       DATCGS010000477         6397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_684399, whole genome shotgun sequence.
ACCESSION   DATCGS010000477 DATCGS010000000
VERSION     DATCGS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684399"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..928)
                     /locus_tag="VIL39_09825"
     CDS             complement(455..928)
                     /locus_tag="VIL39_09825"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5481766.1"
                     /translation="MHELKVEKTTIGRSEDNTFEIADPSVSSHHCEVLLRGSEVVVRD
                     LNSTNGTFISGGKVTEKVLKIGQLLRLGQVEMRLEADAAGAPAKKQFDRTTVITGGVK
                     LTELEQGAHGAGFDTKGSGFSKKDNKVNEIFIIIGVILGLVILALLIYVATMIKK"
     gene            complement(1133..1702)
                     /locus_tag="VIL39_09830"
     CDS             complement(1133..1702)
                     /locus_tag="VIL39_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481767.1"
                     /translation="MAILVAFAGVSLWTGPAHAQETNACLPVPATKLEAFETNTDTVI
                     IKATDPIGTVSVHAGAVSVRCREITDAGTGHRESGIIVDMALGAQLDGTTLIDYDELD
                     SLIDGLDYLGKLDWTVTSLPDFHAVYTTKGGFRADARGSRRTGNIEFTVRSTRVIGPK
                     LSFSRDQLGQLRSLVEQAKTKLDSLRKAK"
     gene            complement(1924..2859)
                     /locus_tag="VIL39_09835"
     CDS             complement(1924..2859)
                     /locus_tag="VIL39_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5481768.1"
                     /translation="MKRICIVASLVLGMVLSSAQEDTITLDDLVQSADQWAKENLDED
                     ALRALQDVDRAKVKQFFSDIQKQFQGEYVMDLASLKDTAKIILPLLEGYEETQPYGAW
                     LKARLDYLEVADQFRRTSPPPKVEPGQPPKPAPNPAPNVERETWNKQLAERPWPVAAK
                     PYVSRLKPIFTGQKVPPELVWIAEVESSFDPRARSPVGAAGLFQLMPATAKRFGLRTS
                     PRDDRLRPEDSARAAAKYLDYLHGQFKDWRLALAAYNAGEGTVQKLLQRHKARTFDTI
                     ATHLPAETQMFVPRVEATLARREGVKLSELRVPPA"
     gene            3051..5879
                     /locus_tag="VIL39_09840"
     CDS             3051..5879
                     /locus_tag="VIL39_09840"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY5481769.1"
                     /translation="MGKALIIAEKPSVAGDIAKALGGFRKHDDYYESDQHVLSSAVGH
                     LLELCVSDQFEVQRGKWSFANLPVIPPHFDLRPIDKSEGRLKLLLKLIKRPDVDALIN
                     ACDAGREGELIFRYVVQYAKAKQPIKRLWLQSMTPAAIREGFEKLRDDASMLPLADAA
                     VCRSESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAILVEREDRIKKFVSKD
                     YWEIHGTFLAKAGEYPGRWFDEKFTKKDGEGDQKPERVWEGKRAEAIRAKCLGKPGIV
                     TEESKPTTQMSGALYDLTTLQREANGRFGFSAKTTLSIAQSLYEKHKVLTYPRTDSRA
                     LPEDYIATVKTTLGMLADTSYGTFAGQILKSGWVKPNKRIFNNAKVSDHFAIIPTSLA
                     PKHLGELEAKLYDMVTKRFLAVFYPAAEFLVTTRITRVEGEPFKTEGKVMTNAGWLAI
                     YGKEAQTDDTPTLAPVLPNETVKTTNVEVIAEQTKPPARFTEATLLSAMEGAGKLVED
                     EELREAMREKGLGTAATRAQVIEGLIYEKYVLRQGRDLQPTAKAFSLITLLRGLEISE
                     LSSPELTGNWEFKLRSMARGQMKRPDFMKEIGDVTRDIVAKAKRHESDTVPGDFGTLK
                     VPCPKCGGEMQENYKKFQCQKCEFSLWKIVAGRQLEIPEVEELIAKRQVGPLQGFRSR
                     LGKPFAAMIRLTPELEPKFDFGQDRLDANGAVAEVDFTGQEPLGKCPKCGQRVFDTPM
                     AYVCEKAAGANPTCDFRSGKLILQRAIEREQMQKLLATGKTDLLEKFISKKGRPFKAF
                     LVVKDGRVGFEFEPREAKPRKASGKEGQPAEPEPKLDFTGQQPLGKCPKCGKRVFEGG
                     AAFVCEQSQAEKRPCKFKINKVILQQPIDREQAARLLAKGKTDLLREFISAKSGRPFA
                     AYLVMDETGKVGFDFPPREDETTKSAVK"
     gene            5876..>6397
                     /locus_tag="VIL39_09845"
     CDS             5876..>6397
                     /locus_tag="VIL39_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY5481770.1"
                     /translation="MRHGDTQPPVNDEWINKFLQHLATDRGASAYTQRNYRQAMLEFH
                     RWHQQERKQPPAWETLQRDDFRAYLRFLGRHNLSRAAIQLRFSALRTFYRFLVRHGAL
                     ASSPIKNLALPKLGKRLPKFLTAQQMADLLAAPLKLLPPPGGSGAAHAAAVFACRRDV
                     AVLETIYSCGLRIS"
BASE COUNT         1303 a         1853 c         2043 g         1198 t
ORIGIN      
        1 cagacgtgga gaggtggaat tatgttcaaa aagcgttaga aaaagagttt ggaccgcaac
       61 catgccattt gcttgcggcc atcttccctg ctcaaattta gcgggagtat tgggtgcttt
      121 gtgggggaga gggcgtattc aggccgtttt gggtcttttg gcgcgaattc gtctcgattc
      181 agatgacaaa cgcctccgga cagggtacta taatgcttcg aattcatcat gatgaacggc
      241 aaacagcgag acgcaacgaa gcttgaggcc gagttgataa gacttaacac cttggttcgg
      301 gaacggcgga agcagttgga gcgcctggac aaatgcccaa acaaggactg tccctgccgt
      361 ttcgtttgga gggagcatgt ggagataggt ctggccggcc aggtgggaat aatccggcgg
      421 caggtgaagg gcaagccagc caaactcagc gagcctactt cttgatcatc gtggcgacgt
      481 agatgagcaa ggccaggatc accaggccaa gaatcacacc gatgatgatg aagatctcgt
      541 tgaccttgtt gtccttcttg gagaagcccg agcccttggt gtcgaatccc gcgccgtgcg
      601 cgccttgctc cagctcggtc aatttcacgc cgcccgttat gaccgtcgtg cgatcaaact
      661 gctttttcgc gggagcgcca gccgcatccg cttccaggcg catttcaacc tgacccaagc
      721 gcagaagctg gccgatttta agcaccttct cggtgacctt tccgccgctg ataaaggtcc
      781 cgttggtgga attcaggtcc cggaccacta cctcgctgcc gcggagcaga acctcgcagt
      841 gatggctgga gaccgacggg tcggcaattt caaacgtgtt gtcctccgac cggccgatag
      901 tggtcttctc cactttcagt tcgtgcatgc gcccggtcat tcccgggctg aggactacaa
      961 gcttcagcat aaatcaccca aacgaagcga ttatggtttg cggccggctg aagtcaagcg
     1021 agagtcacca tcagaatggc cgtcagcagc gccattgagc agaagtgtgg ggcaacgagt
     1081 cctcgtctgt gaggctcccg cctcgcagcg agaggaagga gcgaagcgcg gactattttg
     1141 ctttgcgcaa cgaatcgagc ttggtcttgg cttgctcgac caggcttcgc aactgcccta
     1201 attgatcccg tgaaaacgag agcttagggc cgattacccg ggtgcttcgc acggtgaact
     1261 cgatattgcc cgtgcgcctg ctgccgcggg cgtcagcgcg gaacccccct ttggtggtat
     1321 aaaccgcatg gaagtcgggc aaggaggtca ccgtccaatc gagcttgccg aggtaatcca
     1381 ggccgtctat cagcgaatcc agttcgtcat agtcgatcag cgtcgtgcca tccaactgcg
     1441 cgccgagcgc catatcaaca ataatgccgc tctcccggtg gccagtgccg gcgtcggtga
     1501 tctcccggca tcgcaccgac accgccccgg catggaccga caccgtgcca atcgggtcag
     1561 tggccttgat gataaccgtg tcggtgttcg tctcgaaggc ttccagcttc gtcgctggca
     1621 ccggcaggca agcgtttgtc tcctgagcgt gagccgggcc ggtccacagg gacacgccgg
     1681 cgaaggcaac aagtatggcc atgaatctgc gcatcatctt ttctttgggt ttggacgttt
     1741 caaccaacca actccttcaa tggttcatcc gccagcgcca accagaggac gacttaatcc
     1801 ctggctcacc tctggcgtag ttttctggtt catgagatgg gcgccgtcag cctccaccgc
     1861 agatagggat ttggtgctcg cttgatggaa agcgagatgc tccccccgcc tttgcttccg
     1921 catctacgcc ggaggcacgc gcagttcgct gagtttgacg ccttcgcggc gggccagtgt
     1981 cgcttcgacc cggggcacga acatctgcgt ctcggcgggc agatgcgtgg caatggtatc
     2041 aaacgtccgc gctttgtgcc gctgcaggag tttctggacg gtgccttcgc cggcattgta
     2101 agccgccagg gccaggcgcc agtctttgaa ttgaccatgg aggtaatcca ggtacttggc
     2161 cgcggcacgg gcgctgtcct cgggccgcag gcgatcatcc cgcggcgagg tccgcagccc
     2221 gaatcgtttg gcggtggcgg gcatgagctg aaacaggccc gccgccccaa ccggactgcg
     2281 cgcgcgcgga tcaaacgagg attcgacttc cgcgatccag accagttccg gcgggacctt
     2341 ctgcccggtg aagatcggtt tcagacggga cacgtagggt ttggctgcta ctggccatgg
     2401 ccgctcagcg agttgcttgt tccaggtctc ccgctccaca ttcggcgccg gattgggtgc
     2461 cggcttcggc ggttggcccg gctcgacttt cggcggcgga ctggtgcgcc ggaactggtc
     2521 ggccacttcg aggtaatcca gtcgggcctt gagccaggcg ccgtaaggct gcgtttcctc
     2581 gtacccttcg agcagcggca gaatgatctt ggcggtatct ttgagcgacg caaggtccat
     2641 cacgtattcg ccttggaact gcttctggat gtcactgaag aactgcttca ccttggcgcg
     2701 atccacgtcc tgcaacgcgc gcaacgcgtc ttcgtcaagg ttctctttcg cccactggtc
     2761 ggcggattgg acaaggtcgt cgagggtaat tgtgtcttcc tgggccgatg acaggaccat
     2821 ccccagcacc aaggatgcaa cgatgcagat tctcttcacc gccaccaaaa tagcagggtt
     2881 gcgcgctcaa tacaacccga ctgcacgggg cgctgccagg catgatcctc ggaggaacag
     2941 gtgggccagg gatgcaaaac ctttgacagg gcagaggatt tgcgccactt ttccgccgct
     3001 tgcacagatt tgccacattt gcggacaagg tgtaagtgtc attggaatat atgggcaagg
     3061 cattaatcat tgccgagaag ccgtcggtcg cgggcgacat cgccaaagcg ctgggcgggt
     3121 tcaggaaaca cgacgactac tacgaaagcg accagcatgt cctttcgtcg gcggtcggcc
     3181 atctgctgga gttgtgcgtg tcggaccaat tcgaggtcca gcgcgggaag tggtcgtttg
     3241 ccaatctgcc ggtgattccg ccgcacttcg acctgcggcc gatcgacaag agcgaggggc
     3301 ggctcaagct gctgctcaag ctgatcaagc gcccggacgt ggacgcgctg atcaacgctt
     3361 gcgacgccgg ccgggagggg gagctgattt tccgctacgt ggtgcaatac gccaaggcca
     3421 agcagcccat caaacgcctg tggctgcagt ccatgacgcc ggcagccatc cgggaagggt
     3481 tcgagaaact gcgagatgat gcttcgatgc tcccgctagc ggatgcggcg gtgtgccgtt
     3541 cggaatcgga ttggctgatt ggcatcaacg gcacgcgggc gatgacggcg ttcaactcga
     3601 agacgggcgg cttccatctg acgacggtgg gccgggtgca gacgccgacg ctggcgatcc
     3661 tcgtggagcg ggaagaccgc atcaagaagt tcgtctccaa ggattactgg gagatccacg
     3721 gcacctttct ggccaaagca ggcgaatacc cggggcgctg gttcgacgag aagttcacca
     3781 agaaagacgg cgagggcgac cagaagccgg agcgggtttg ggagggcaag cgggcggagg
     3841 ctatccgcgc caaatgcctc ggcaagccgg gtattgtcac cgaagaaagc aaacccacga
     3901 cgcaaatgtc gggggcgctc tacgatctga cgaccttgca gcgggaggca aatggccggt
     3961 tcgggttttc ggccaagacc acgctctcca tcgcgcagtc gctctacgaa aagcataagg
     4021 tgctcacgta tccgcgcacg gactcgcggg cgctgcccga ggattacatc gctacggtca
     4081 agacgacgct cgggatgctg gcggacacca gctatggcac gttcgccggc cagattctca
     4141 aaagcggctg ggttaagccg aacaagcgca tcttcaacaa cgccaaggtc tcggatcact
     4201 tcgccatcat cccgacctcg ctcgcgccca agcatctggg ggagctggaa gcgaagcttt
     4261 acgacatggt caccaaacgg ttcctggcgg tgttttatcc ggcggcggag tttctggtga
     4321 cgacgcgcat cacgcgggtg gagggtgagc cgttcaagac cgagggcaag gtgatgacga
     4381 acgcgggctg gctggccatc tatggcaagg aggcccagac cgacgacacg ccgacactcg
     4441 cgccggtgct gccgaacgag acggtaaaga ctaccaacgt cgaggtgatt gccgagcaga
     4501 ccaagccgcc ggcgcgcttc acggaagcga cgctgctcag cgcgatggaa ggcgcgggca
     4561 aactggtcga ggacgaggaa ttgcgcgagg cgatgcgcga aaaagggctg ggcacggccg
     4621 cgacgcgcgc acaagtcatc gaaggactga tctacgagaa atacgtgctg cggcagggac
     4681 gcgacctcca gccgaccgcc aaggcgtttt ccctgatcac gctgttgcgc gggctggaga
     4741 tttccgagct gtcctcgccg gaactgaccg gcaactggga gttcaagctg cgcagcatgg
     4801 cgcgcgggca aatgaagcgg cccgatttca tgaaggagat tggcgacgtc acccgcgaca
     4861 tcgtcgccaa ggccaagcgg cacgagagcg acacggtgcc aggcgatttc gggacgctga
     4921 aggtcccgtg ccccaagtgc gggggcgaga tgcaggagaa ctacaagaag ttccagtgcc
     4981 agaagtgcga gttcagcctg tggaagattg tggcgggccg ccagctcgaa atccccgagg
     5041 tcgaggaact cattgccaag cgccaggtcg ggccgctgca aggttttcgc agccggttgg
     5101 gcaagccttt cgcggcgatg atcaggctca cgccggagtt ggagcccaaa tttgatttcg
     5161 gccaggaccg gctggatgcg aatggcgcgg tggcggaagt cgatttcacg gggcaggaac
     5221 cgctggggaa gtgcccgaag tgcggccagc gcgtattcga tacgccgatg gcttacgttt
     5281 gtgagaaggc agccggggcc aaccccacgt gcgatttccg gtcgggcaag ctcatcctgc
     5341 agcgagccat cgagcgcgag cagatgcaaa agctgctcgc caccggcaaa acggacctgc
     5401 tggagaagtt catttccaag aaaggcaggc cgttcaaggc cttcctggtg gtcaaggacg
     5461 ggcgcgtcgg ttttgagttc gagccgcgcg aggcaaagcc ccggaaagcg tccggcaagg
     5521 aagggcagcc ggcggagccg gagccgaagc ttgatttcac gggccagcaa ccgctgggca
     5581 aatgccccaa gtgcgggaag cgggtgttcg aaggcggcgc ggctttcgtg tgcgagcagt
     5641 cgcaagcgga gaagcggccg tgcaagttca agatcaacaa agtgatcctg cagcagccca
     5701 ttgaccggga acaggcggcg cggctgttgg caaagggcaa gacggacttg ttgcgcgagt
     5761 tcatttccgc caagagcggc cggccgttcg ccgcgtacct ggtgatggac gaaacgggca
     5821 aggttggttt cgatttcccg ccgcgtgagg acgagacgac aaagagcgcg gtgaagtgag
     5881 gcacggagat actcagccac cggtcaacga cgagtggatt aacaagttcc tgcagcacct
     5941 ggctacggat cggggagcct cagcctacac gcagcgcaac taccggcagg cgatgctgga
     6001 gtttcatcgc tggcatcagc aggagcggaa gcagccaccg gcttgggaga cactgcaacg
     6061 tgatgatttc cgcgcctacc ttcgatttct ggggcgacac aatctcagcc gggcggccat
     6121 ccagctccgc ttcagcgcct tgcgcacgtt ttaccgtttc ctggtccggc acggggcgct
     6181 ggcaagttca ccgatcaaaa acctcgccct gcccaagctt ggaaagcgcc tgccgaaatt
     6241 cctaaccgcg caacagatgg ccgatttgct tgccgccccg ctgaagctct tgccgccgcc
     6301 gggcggaagc ggggccgccc acgctgccgc ggtcttcgcc tgccggcgcg atgtggcggt
     6361 gctggagacc atttattcgt gcggcttgcg catcagc
//
LOCUS       DATCGS010000478         3082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_385877, whole genome shotgun sequence.
ACCESSION   DATCGS010000478 DATCGS010000000
VERSION     DATCGS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385877"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..>3082
                     /gene="secA"
                     /locus_tag="VIL39_09850"
     CDS             353..>3082
                     /gene="secA"
                     /locus_tag="VIL39_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5481771.1"
                     /translation="MIGYIVKKFIGSKNDREVKRLRPLVAKINAFEAGLQKLSDDDLR
                     QKTAAWKEEVSKIQDYEELAQRLTEILPEAFAVVKDACRRLCGKDIIVREHPLPWEMV
                     PFDVQLIGGYALHAGKIAEMATGEGKTLVATLPVYLNALTGRGVHVVTVNDYLAARDS
                     EWMGAVYKFLGLTVGCILHDQSPSVRREQYNCDITYGTNAEFGFDYLRDNGMATRKEE
                     QVQRGHYYAIVDEVDSILIDEARTPLIISGPSVVTHDEQYANFRAQVEGIYHVQERLC
                     SRFLSEAQELLGKLRPEDGSNVENREAMERQAGLLLFRVKCGQPKSEGLAKVLEEPEN
                     LRLMNHAELQLHADQKKVELYAEKEELFFAIDEKSHEADLTEKGRNYLSPKDPDAFML
                     PDLTTALHDIDVGPEADPRKRLEAKTKLQQEFETKAQRIHAIGQLLKAYCLYQKDVQY
                     VVQDNKVIIVDENTGRLMSGRRWSDGLHQAVEAKEQVEIERETQTLATITIQNYFRLY
                     HKLAGMTGTAETEASEFFDIYKLGVLVIPTNKPVARSDANDSVYKTRREKYNAVLKEI
                     KEVHGKGRPMLVGTISVEVSEHLSRMLKREGIIHSVLNAKYHQQEAEIVTRAGQRGSV
                     TIATNMAGRGTDIKLGAGVAEVGGLHVIGTERHEARRIDRQLRGRCARQGDPGSSHFF
                     ISLEDDLMRLFGSDKIVKYMEKMGLEEGQELEHPLLNRSIGQAQKRVEQHNFQIRKRT
                     LEYDDVMNKQREVIYGFRNEIIHGADVRDRLMDIMEEVVLQKVEQLTSGTNDFNEWKV
                     RPLSDWVNLNFPIGMPEKEIIKAAEFGKEEPVKGSVFDNLSPAQFAVCSFISDSVRKA
                     YEVKISFENPEALKEVERYTILSAIDKLWQEHLYEMDSLRYSIGLRAHGQRDPL"
BASE COUNT          679 a          909 c          955 g          539 t
ORIGIN      
        1 cggagctgtg cccgcgggcc agcacctcat ctcaccgaga acagcgaaga acctgcaacc
       61 cggtctccgg aagggcgagt gtccccgcga gcccaaatat ctcccgaatt ctggcaagtc
      121 ggcgcttgcg aggacgctcg ctctcccggc ggagatttcc ttggcgtggc cgggcaggtg
      181 gggtcgaggc ggtctggctg tggcggctgg tgggcggttg ggcgagggct tgggcccacg
      241 ggagcggaat attgtcgcgc gagggacgat tcgtgggtca aggcattagc cccttaatgt
      301 ctttgacgcg cggggatggt tcccgtagcg tctgggagta gttaaatcaa tcatgattgg
      361 gtacattgta aagaaattca tcggctcgaa gaacgaccgg gaggtcaaac gcctccgtcc
      421 cctggtcgcg aagatcaacg ccttcgaggc cggactgcaa aagctctccg acgacgactt
      481 gcgccaaaag accgctgcct ggaaagagga agtgtccaaa atccaggact atgaggagct
      541 tgcccagcgg ctgactgaaa tcctgcccga ggccttcgcc gtcgtgaaag atgcatgccg
      601 gcggctttgc ggcaaggata tcattgtccg cgagcatccg ttgccgtggg aaatggtccc
      661 tttcgacgtg cagctcatcg gcggctatgc cttgcacgcg ggtaaaattg ccgaaatggc
      721 taccggcgaa ggcaagaccc ttgtggccac gctcccggtc tatctcaacg cgctgactgg
      781 ccgtggcgtt cacgtcgtca cggtgaacga ttacctggcc gcgcgcgaca gcgagtggat
      841 gggcgcggtg tataagtttc tcgggctgac ggtcggctgc atcctgcacg accagtcgcc
      901 cagtgttcgc cgcgagcagt acaactgcga catcacctac ggcacgaatg ccgagtttgg
      961 cttcgactac ctgcgcgaca acggcatggc gacgcgcaag gaggagcagg tgcagcgggg
     1021 ccattactac gccatcgtgg atgaggtgga ctcgatcctc attgatgaag cgcgcacgcc
     1081 gctcatcatc agcgggccgt ctgtggtcac gcatgatgag cagtacgcca atttcagggc
     1141 gcaggtcgaa gggatttacc atgtgcagga gcggctgtgc agtcggttcc tgtccgaggc
     1201 gcaggagctc cttggtaagc tgcgtccgga agacggttct aatgtggaga atcgcgaggc
     1261 gatggaaagg caagctggcc tgctgctctt ccgtgtcaaa tgcggccagc ccaagtccga
     1321 gggcctggca aaagtgctgg aagagccgga gaatctccgg ctcatgaacc atgccgaact
     1381 gcagcttcac gccgaccaga agaaggtcga gctctacgcg gagaaggagg agcttttctt
     1441 cgccattgac gagaagagcc acgaggcgga tctgaccgag aagggccgca attacctgag
     1501 cccgaaggac ccggatgcct ttatgctgcc cgacctgacc acggcgctgc acgatattga
     1561 tgtcgggccg gaagccgacc cgcgcaagcg gctcgaggcg aagaccaaac tccagcagga
     1621 gttcgaaacc aaagctcagc gcatccacgc gattgggcag ttgctcaagg cctactgcct
     1681 gtaccagaag gacgtccaat atgttgttca ggacaacaag gtcatcatcg tggacgagaa
     1741 caccggccgc ttgatgagcg gtcgccgctg gagcgatggt ctccaccagg ccgtcgaagc
     1801 caaggagcag gtcgagatcg agcgcgagac gcaaacgctc gccaccatca ccattcagaa
     1861 ctacttccgc ctctaccaca agctggccgg catgaccggc acggcggaaa ccgaagcttc
     1921 ggagttcttt gatatttata aactcggcgt gctggtcatt cccaccaaca agccggtggc
     1981 gcgcagcgac gcgaacgatt cggtctataa gacccgccgg gagaagtaca acgcggtgct
     2041 caaagagatc aaggaagtcc acggcaaagg ccggccgatg ctcgtcggca cgatttcggt
     2101 ggaagttagc gagcatctgt cgcgcatgct gaagcgggaa gggatcatcc actccgttct
     2161 caacgccaag tatcaccagc aggaagcgga aatcgtcacg cgtgccggcc agcgcggttc
     2221 cgtgaccatc gccacgaaca tggccggccg cggcaccgac atcaaactcg gcgccggcgt
     2281 ggctgaggtg ggcggtctgc acgtcatcgg caccgagcgc cacgaggccc gccgcattga
     2341 ccgccagttg cgtggccgtt gcgcgcgcca gggcgacccc ggctcctcgc acttcttcat
     2401 ctcgctcgaa gacgatttga tgcggctgtt cggctccgac aagatcgtca agtacatgga
     2461 gaagatgggc ctcgaagaag gccaggaact cgagcacccc ctgctcaacc gctccatcgg
     2521 gcaggcccag aagcgcgtcg aacagcataa cttccaaatc cgcaagcgca cgctcgaata
     2581 cgacgacgtg atgaacaaac agcgcgaagt catttacggc ttccgcaacg agatcatcca
     2641 cggcgccgat gtccgcgacc gcctgatgga catcatggag gaagtcgtcc tgcagaaggt
     2701 tgagcaactc acttccggga cgaacgactt caatgagtgg aaagtgcgcc cgctctccga
     2761 ctgggtgaac ctcaacttcc ccatcggcat gccggagaag gagatcatta aagccgccga
     2821 attcggcaaa gaagagccgg tcaaaggctc ggtcttcgac aacctcagcc cggcgcagtt
     2881 tgccgtatgc agtttcattt ccgacagcgt ccgcaaggct tacgaggtca agatcagctt
     2941 cgagaacccc gaggcgctca aggaggttga gcgctacacc atcctgagcg cgattgacaa
     3001 gctgtggcag gaacatctct acgagatgga tagcctgcgt tacagcatcg ggctgcgcgc
     3061 gcacggccag cgggacccgc tc
//
LOCUS       DATCGS010000479         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_90334, whole genome shotgun sequence.
ACCESSION   DATCGS010000479 DATCGS010000000
VERSION     DATCGS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90334"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..>2792)
                     /locus_tag="VIL39_09855"
     CDS             complement(292..>2792)
                     /locus_tag="VIL39_09855"
                     /inference="COORDINATES: protein motif:HMM:NF028151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HEY5481772.1"
                     /translation="RTIHTFNDRMFSSDLNLRWRAGTGAWQSLAFTLPAAGQRQDSIT
                     FTVPAAVGAFDLQYELSNSNNVVYTNTVACSAMASTSLTLPVGVTAGLYDPKGTTAGL
                     LNRFGIAFAAVTDLRTNSLSQFNLLIVGRDALTNDTIAEAGNSTVASRWKDFARGGGW
                     VLVLEQTNYPSWMPAELALQNYDASFAFPSQDHPVMQGVAAADLRWWAGDHRVVLKSI
                     QLPSRYNVRALASVGSHQGLNLAAAAEVPLGSGGILCSQWLLTQRFDQEPLAGLLLQR
                     LLNYCAPGCPHPALKPIALVGETNAAAAAKLTELSVLAENVSGRLAGLTPALYPVLMI
                     AGGSNAWQEAGAHIADLAAYVSAGGKLVLHRPSAAFLSAAQPALFPELNYTDAQLGAV
                     LRRDVTNPAVRLASDDLYWIDQAGDWNHTETLSTNVASRYYRKKFNLATYSTIQVENM
                     PIHTSGGASSGGWFLWVNGYVAQNINVTQAGTYLFNVFASGTPALGGWPIMTLKIDGL
                     AQDSITVPTNQVAAYTLMADLTPGTHQLAISYDNDAYAPPEDRNLFLDQIQWGREADN
                     SPTSLLTRPGAVAQVRHGAGVVILDEIGWETETKNAAKAGRYACRLLTGLGGACQLPN
                     SLTIEAEAMTNVNVSAYNVSGGITWLYSNGRIESQVRITTAGNYTFEVFAGGTTAQGV
                     YPQVAITVNGANRATWFLTSTNMTRYAFTIYLGVGTYKLGLAFLNDANPAPEDRNAAF
                     DRLTLTPETAFRIVNWSADSFSQTFSVQWECLPFKPYEVQICTNLAGGPWQAATNFTV
                     SGNIGSWTDTGFAGAPPLNPDAPERFYRVRQSGP"
BASE COUNT          504 a          829 c          928 g          531 t
ORIGIN      
        1 ggacggctga cggatcgcac actgacggat cgcacagggt ttcagccgtc ccttcgggac
       61 ttgtgcccct ctaaactcgc gcccccaacg ttgaaacgtt gggctattct cggccatccc
      121 tccgggatga ggagggtcaa atcctagcgg cattggacag gaatgtccgc gcgccggtgg
      181 ttgtgccgag ataggcccga cgggtttcac gcttcgacgt tacctctccg ccctcgcgct
      241 cgcctgtccc gccagcacct gcctcgtttc ccctgcaagc tggcaactcc ttcaaggacc
      301 gctttggcgg acgcggtaga agcgttccgg agcatcgggg ttcaaaggcg gcgccccggc
      361 aaacccggtg tcagtccagc ttccgatgtt cccggagaca gtgaaattgg tcgcggcctg
      421 ccacgggccg cccgccaggt tcgtgcaaat ctgcacctca tagggcttga aggggagaca
      481 ctcccactgc actgaaaacg tttgtgagaa agagtcggcg ctccaattga cgatgcggaa
      541 agccgtctcg ggcgtaaggg tgagccggtc gaaggcggcg ttgcggtctt ccggcgcggg
      601 attggcgtcg ttaagaaatg ccaggccgag cttgtaggtg ccaaccccca ggtaaatggt
      661 gaatgcgtat cgggtcatgt tcgtgctggt cagaaaccaa gtggcgcgat tcgcaccgtt
      721 caccgtgatg gcgacttgcg gatagacccc ctgggcggtg gtgccgccgg cgaacacctc
      781 gaacgtgtaa ttccccgcgg tggtaatgcg gacctggctt tcaatgcggc cattgctgta
      841 gagccaagtg atgccgcccg aaacattata ggcgctcacg ttcacgttgg tcatggcctc
      901 ggcctcgatg gtcagcgagt tcggcaactg gcacgcgccg cccaagccgg tcaaaagccg
      961 gcaggcgtag cggcccgctt tggccgcgtt cttggtttcc gtttcccagc cgatttcgtc
     1021 caggatcacc actccggcgc cgtgccgcac ctgcgcgacg gctcctggcc gcgtcagcag
     1081 cgaggttggg ctgttgtcag cctcgcgccc ccactgaatc tggtccagga acaggttgcg
     1141 atcctccggc ggggcatagg catcgttgtc gtaggagatg gcgagttggt gggtgccggg
     1201 ggtgagatcg gccatgaggg tgtaagcggc gacctggttg gtgggcacgg taatcgagtc
     1261 ttgcgccagg ccgtcaatct tgagcgtcat gatcggccag ccgcccagcg cgggtgtgcc
     1321 gctggcaaat acattgaaaa ggtaagtccc ggcctgggtg acattgatgt tttgcgcgac
     1381 gtagccgttg acccacaaga accagccgcc tgaactggca ccgccgctgg tgtgaatggg
     1441 catgttctcg acctggatcg tgctgtaggt ggccaggttg aacttcttgc ggtagtagcg
     1501 gctggcgaca ttggtggaga gcgtctcggt gtggttccaa tcgccggcct ggtcaatcca
     1561 ataaaggtcg tcactcgcga ggcgcacggc tgggttggtg acgtcgcgcc gcagcaccgc
     1621 gccgagttgc gcatcggtgt aattcagttc gggaaaaagc gccggctgcg cggccgagag
     1681 gaatgcggcg ctgggtctat gcagcacaag cttgccgccg gcactgacgt aggcggcgag
     1741 gtccgcgatg tgggcgccgg cttcctgcca cgcgttgctg ccgccggcga tcatcagcac
     1801 cggataaaga gccggagtca agccggccag ccggcctgaa acattctcgg ccagcacact
     1861 aagctccgtg agcttggccg ccgccgccgc attggtctcg ccgacgagag cgatcggttt
     1921 cagggctggg tgcggacaac ccggggcgca atagttcagc aggcgttgca acagcagtcc
     1981 agccaggggc tcttgatcaa aacgttgcgt cagcagccat tgcgagcaga ggatgccgcc
     2041 ggagccgagc ggcacttccg ccgcggcggc gagattcaag ccttggtggg agccgacgct
     2101 ggcgagcgct cggacattgt agcggctggg cagttgaatc gatttgagga cgacccggtg
     2161 gtcgccggcc caccagcgca ggtccgcggc ggccacgccc tgcatgaccg gatgatcctg
     2221 gctgggaaac gcgaagctgg catcgtaatt ctgcaaggcc agttcggccg gcatccacga
     2281 gggataattg gtttgctcaa gcaccaggac ccatccgccg ccgcgcgcga agtctttcca
     2341 acggctggca acggtgctgt tgcccgcctc ggcgatggtg tcattggtca gcgcgtcgcg
     2401 gccgacgata agcaggttga actggctgag cgagttggtc cgcaaatcgg tgacggcggc
     2461 aaaagcaatc ccgaagcggt tcagcaaacc ggcggtggtg cctttgggat cgtaaagccc
     2521 ggccgtcacg ccaaccggca gggtcaaact tgtgctcgcc atagcggagc aggccacggt
     2581 gttggtgtaa acgacgttgt tcgaattgct cagctcgtat tgcagatcga aagcgcccac
     2641 cgccgccggc acggtgaacg taatgctatc ctgccgttgt ccggcggcgg gcaaggtgaa
     2701 tgccagactt tgccacgcgc ctgttcccgc gcgccagcgc aggttcaaat cgctcgagaa
     2761 catgcggtcg ttgaacgtat gtatagtgcg tg
//
LOCUS       DATCGS010000480         3792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_39057, whole genome shotgun sequence.
ACCESSION   DATCGS010000480 DATCGS010000000
VERSION     DATCGS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39057"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1478
                     /locus_tag="VIL39_09860"
     CDS             75..1478
                     /locus_tag="VIL39_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5481773.1"
                     /translation="MNDNNGLPLVTPRVSPVLDPAFRPAVLANRAFRAQARATRNAVT
                     VRLGLEQTDGNVSHFTTPILPDSHPQAAGNFTYLERITKFLLWTRGGFRIHFEGPAPL
                     AAKLAAHYRETAMGKFDSKLVGEGMFDHPIEVVHTKALPPERRSAGSLGRHLDGCRIG
                     FDLGGSDRKVAAVIEGKVVFSDETAWDPYFQPDPQYHYDGIMDSLKKGAAHLPRVDAI
                     GGSAAGIYVNNRVKVASLFRGVPPDVFNRRVKDLFLDIRKAWNNVPLEVVNDGEVTAL
                     AGSMSLGLNRILGISLGTSTAGGYVNAEGNITSWINELAFVPVDYNPQAATDEWSGDY
                     GCAVQYFSQQCVGRLLAPAGIEVDAKLPLPEKLKHVQKLMDKGDDRARKIYQTIGTYL
                     GYAIPHFTDFYDLENVLILGRVTSGPGGDIIIAGAKEVLKAEFPELTGKIAFHIPDEK
                     DKRHGQAVAAASLPKAG"
     gene            1511..2368
                     /locus_tag="VIL39_09865"
     CDS             1511..2368
                     /locus_tag="VIL39_09865"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5481774.1"
                     /translation="MTPYHKLVSDYARLLKKGRSYPLGTFEPATRPEIPTNAPKALFF
                     APHPDDECIVGGMAVRLMRQARMNLINVAVTLGSKKERQQERLRELQNACNYIGFGLV
                     TTAPNGLERINPKTREQDPKHWAACVKIIKDLIEQHQPKVVLCPHDRDWNSTHIGTHY
                     LVMDALKQMPASYECYVVETEFWGAMSDPNLMVEISAEDLADMIAATTFHVGEVARNP
                     YHLLLPPWMMDNVRRGGEVAGGQGGAAPDYAFAVLYRLRKWSKGQATKMFEGGKQVPL
                     SMNVAELFA"
     gene            2365..2832
                     /locus_tag="VIL39_09870"
     CDS             2365..2832
                     /locus_tag="VIL39_09870"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY5481775.1"
                     /translation="MKEKFMREAIRLSIQMMRRGQGGPFGAVVVRNGKVVGRGSNQVT
                     SANDPTAHAEIVAIREACKRLKTFQLDDCDLYTSCEPCPMCLSAIYWARLANVFYANT
                     RKDAAKINFDDDFIYREVALPIRKRTLKMKQVLHDEALAAFTEWQQKTDKVLY"
     gene            complement(3092..>3792)
                     /locus_tag="VIL39_09875"
     CDS             complement(3092..>3792)
                     /locus_tag="VIL39_09875"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HEY5481776.1"
                     /translation="SLEEACPADKLAALQATNPNFYTITYINCSAAVKALSDVICTSG
                     NAVKIVQAAPKDKDLLFVPDENLGEWVMEQTGRPMTLWKGYCYAHVEFRRDSLLRAKQ
                     SFPQAKVVVHPESLHEVRELADVVCSTEKMITYCQSSPAKQFVVVTESGIIHRLQKEC
                     PGKEFVCAPTFDVMRAPTDPCRCSECKYMKLNTLEKLRDCMKNLAPRIELSAEIIRRA
                     QLPIQRMLEISARP"
BASE COUNT          748 a         1195 c         1141 g          708 t
ORIGIN      
        1 ggagcatccc ggtctgcctt ctaatctgtg cgcttgaatt tggcgtgcgt ctgaattaca
       61 acacctcctg caaaatgaac gataataatg gtctcccttt agttacgccg cgagtttcac
      121 cggtgctcga ccctgcgttc cggcccgcag tcctggccaa ccgcgctttt cgcgcgcagg
      181 cgcgggccac gcgcaacgcc gtgaccgtcc gcctgggcct ggaacagact gacggcaacg
      241 tctcccattt cacgaccccg atcctgccgg acagccatcc gcaggcggcg ggcaatttca
      301 cttacctcga acgcatcacg aagttcctgc tctggacgcg cggcggattc cgcattcatt
      361 tcgaagggcc ggccccgctg gccgcgaaac tggcggcgca ttaccgcgag accgccatgg
      421 gcaagttcga ctcgaagctc gtgggcgagg ggatgttcga tcacccgatc gaggtggttc
      481 atacgaaagc cctgccgccc gaacgccgca gtgccggttc gctgggccgc catctggacg
      541 ggtgccgcat cggcttcgac ctcggcggca gcgaccgcaa ggtggcggcg gtcatcgaag
      601 gcaaggtcgt gttcagcgac gaaaccgcct gggacccgta ttttcagccc gaccctcagt
      661 atcactatga cggcatcatg gattcgctca agaagggggc ggcccacttg ccgcgggtgg
      721 atgccattgg cggcagcgcg gcggggattt atgtcaacaa ccgcgtgaag gtggcgtcac
      781 tgttccgcgg cgtgccgccg gatgtgttca atcggcgggt caaggatttg ttcctcgaca
      841 ttcggaaggc ttggaacaat gtcccgctgg aggtggtgaa tgacggcgag gtcaccgcgt
      901 tggctggctc catgtcgctc gggttgaacc gcatcctggg catttcgctc ggcacaagca
      961 ccgcaggcgg ctacgtcaat gccgagggca atatcacttc ctggatcaac gagctggcct
     1021 tcgtgccggt ggattacaat ccccaggcag cgaccgacga atggtccggc gactacggct
     1081 gcgccgtgca atacttctcg cagcaatgcg tgggccgcct gctcgcgccc gcgggcatcg
     1141 aggtggacgc caaactgccg ctccccgaga agctaaagca cgtccagaag ctcatggaca
     1201 agggggatga ccgcgcccgc aagatctatc agaccatcgg cacctacctc ggctacgcta
     1261 tcccgcactt taccgatttc tacgacctgg agaatgtgct gatcctgggc cgggtcacct
     1321 ccggtccggg cggcgacatc atcatcgccg gagcgaagga ggtgctgaaa gccgagttcc
     1381 ccgagctaac aggcaagatt gccttccaca ttcccgacga aaaagacaag cggcatggcc
     1441 aggcggtagc ggcggcgagc ctgccgaagg cggggtagtt tagtgaattg aaacgccgca
     1501 cgttaattat atgactccct atcacaaact cgtatccgac tacgcgcgcc tcctcaaaaa
     1561 gggccgctct taccccctgg gaaccttcga gcccgccacg cggccggaga ttcccaccaa
     1621 cgcccccaag gcgctgttct tcgcgccgca cccggacgac gaatgcatcg tcggcggcat
     1681 ggcggtgcgc ctgatgcgcc aggcgcgcat gaatctcatc aacgtagccg tcaccctcgg
     1741 cagcaagaaa gagcgccagc aggaacgcct gcgcgagctg caaaacgcct gcaactacat
     1801 cggcttcggc ctggtgacca ccgcgccgaa tgggttggag agaatcaatc ccaagacccg
     1861 cgagcaggac ccgaagcatt gggccgcctg cgtcaagatc atcaaagacc tcatcgagca
     1921 gcaccagccg aaggtggtct tgtgcccgca cgaccgcgat tggaacagca cacacatcgg
     1981 cacccattac ctggtaatgg acgcgctgaa acagatgccc gccagctacg aatgttacgt
     2041 ggtcgagacg gagttctggg gcgcgatgag cgatcccaac ctgatggtcg agatcagcgc
     2101 ggaagatctg gcggacatga ttgccgcgac gacgttccac gtaggcgagg tggcccgcaa
     2161 tccctaccat ttattgctgc ccccgtggat gatggataac gtgcgccggg gcggcgaagt
     2221 agccggcggc cagggcgggg ccgcgcccga ttacgcgttt gcggttctct accgtctgcg
     2281 caaatggagc aaaggccagg cgacgaagat gttcgaaggc ggcaagcaag tgcctctgtc
     2341 aatgaacgtc gctgagctct tcgcatgaag gagaagttca tgcgggaggc catccgcctc
     2401 tccattcaaa tgatgcggcg cggccagggc ggtcccttcg gcgcggtggt cgtgcggaac
     2461 ggcaaagtcg tcgggcgggg ctccaaccaa gtcacctcgg cgaacgaccc gaccgcccac
     2521 gccgaaattg tcgccatccg cgaggcttgc aagcgcctga agacgttcca gttagacgat
     2581 tgcgacctct acaccagttg cgagccgtgc ccgatgtgcc tgtccgccat ttactgggcg
     2641 cggctggcca atgtcttcta cgccaacacc cgcaaggacg ccgcgaagat caacttcgac
     2701 gatgacttca tctaccggga ggtcgcgctg cccattcgga agcgcacgct caagatgaag
     2761 caagtcctgc acgacgaggc cctcgccgcg tttaccgagt ggcagcagaa aacggataaa
     2821 gtcttgtatt gaacctggtc tgctctcggc ggtggaaaca gagcttcagc gccatgcgtc
     2881 gagaccggaa agggcgaacg cccaaagtat tcagtccaag ccgacccttc tcacgcaaag
     2941 tccgccaagg acgcaaagag cagaaaagac tcgggcttca atagtactcc cccaaatggt
     3001 gagcggacgt tcgcccgaca ttctgcccct ttcacttttc tttgcggcct ttgcgacctt
     3061 tgcgtgaggt ttccctctgc attgatacgg cttagggacg ggctgagatt tccagcatcc
     3121 gctggatcgg caactgggcg cggcggatga tctccgcgct taactcgatg cgcggtgcga
     3181 ggttcttcat gcagtccctc agcttctcca gcgtgttcaa cttcatgtac ttgcactcgc
     3241 tgcaccggca tgggtcggtc ggcgcgcgca tcacgtcgaa ggtgggcgcg cagacgaatt
     3301 ccttgcccgg acactccttc tggagccggt ggatgatgcc ggactcggtc accacgacga
     3361 attgcttcgc ggggctggat tggcagtagg tgatcatctt ctccgtcgag cacaccacat
     3421 cggccagctc gcgcacctcg tgcaggctct cggggtgcac caccactttg gcttgcggga
     3481 aagactgctt cgcgcgcagc aggctgtccc gccggaactc cacgtgcgcg taacagtagc
     3541 ctttccagag cgtcatcggt cttccggtct gctccatcac ccactcgccg aggttctcat
     3601 ccggcacgaa cagcaggtcc ttgtccttgg gcgcggcctg gacgattttc acggcgttgc
     3661 cgctggtgca aatcacgtcg ctgagcgcct tcacggcggc gctgcagttg atataggtga
     3721 tcgtgtagaa attggggttc gtcgcctgaa gcgccgccaa cttatccgcc gggcaggcct
     3781 cttcgagcga gc
//
LOCUS       DATCGS010000481         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_487238, whole genome shotgun sequence.
ACCESSION   DATCGS010000481 DATCGS010000000
VERSION     DATCGS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487238"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIL39_09880"
     CDS             <1..273
                     /locus_tag="VIL39_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481777.1"
                     /translation="RAAACEPATPTLPRLANHHPLVQKAVGGIQTEQITTGGQLGKPS
                     SARRRVYERLKDYAAQVKDSLFDIKPLHHAIDDIYESPLGECRRSC"
     gene            complement(842..>2661)
                     /locus_tag="VIL39_09885"
     CDS             complement(842..>2661)
                     /locus_tag="VIL39_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY5481778.1"
                     /translation="GDIGTSPLYALRECFAGSHPLPPTRPNVLGILSLIFWSLLLIVS
                     LKYLAIVLRATNRGEGGILALMALAFPRQSPGASRRRALLIGLGVFGAALLYGDGMIT
                     PAISVLSAVEGLNVATPAFEHYVIPITVVTLIGLFTAQSRGTARVGAVFGPIMVLWVV
                     SLGLLGFRGILMSPSVLWSLDPLLGLEFLFRSGWTGFVVLGSVFLAVTGAEALYADVG
                     HFGARPIRIAWFGLVLPGLFLNYLGQGALILHNPTSIENPFYLLAPHWGLYLLVGLAT
                     AATVIASQALISGAYSLTIQAIQLGYLPRMAVKHTSSMERGQIYLPHVNWALMLACIG
                     LVIGFRSSSNMASAYGIAVTLTMLSTTVLFFFAARRVWGWSAWRAAAACSVFLVVETA
                     FFAANLLKVLKGGWFPLAMGLAIFLLMATWKKGRLLVWNKLRPASMPLEMFLDDIERN
                     RAVPRVPGTALFTTANPEGTPIALLHNLKHNKVIHQRNIILTILTDEVPVVDAAKRLE
                     VTKLAAGFQRIIAHYGFMEEPNVPELLAVAPLVGEPINIHRTTFFLSRETIVPAGSGS
                     MTRWRQWLFAVMSRNAQSAGSFYRIPPNRVVELGMQVEI"
BASE COUNT          611 a          784 c          813 g          453 t
ORIGIN      
        1 cgcgccgccg cgtgcgagcc ggccacgccc acgctcccgc gcctggccaa ccaccatcct
       61 ctcgtgcaga aagccgtcgg cggcatccag accgagcaaa tcaccaccgg cggccagctc
      121 ggcaaaccct ccagcgctcg ccgccgcgtc tatgaacggc tcaaggacta tgccgcccag
      181 gtgaaggaca gcctcttcga catcaagccc ctccatcacg cgattgacga catctacgag
      241 tcgcccctgg gagagtgccg taggagttgt tgaatccgac tgcatccggg ccgaatatca
      301 aagttgcgtt gtaacctgcc gcccaatcgc cctctccagg aggcttgctg atggtgagcg
      361 agataacgtc caggttgaaa ccgtccgcct tcgagccgct cgtcgcccca ttgggcgtgt
      421 agagtggcag attttggtta cctgttcccg ggttccactg catggaggcg tccacgtagc
      481 cgctaattgt tgtggtcgat agcgcggttg ataaagcagt cgggtgttcg tcagcatgtg
      541 ccagtgcggg aagactgact attcccgcgc caattagggc caaagtccat gcattgtatt
      601 tcatagtctt aagttctgct cgttccggct gtggccgcag ccaaacggcg gctcagcagc
      661 cacgccagga acatgatgcg aggccgaatg cacgggccat tccaatgcga cggaaaagac
      721 gcaacctgtt tcacatcagt cacttagaac agtgcgaggc aattggagga ggaaaaagtt
      781 gtgccgattg ctgcgctggg cggtcaaaaa tcgccacaag aaaccgcttc gctcgggaag
      841 gtcagatttc cacctgcatt cccaattcga cgacccggtt gggagggatg cgatagaagg
      901 agccggcgga ttgggcgttg cgggacatta cggcaaatag ccattgccgc caacgggtca
      961 tggaaccgga cccggcgggc acgatagttt cccggctgag gaaaaaggtg gttcggtgga
     1021 tgtttatggg ttcgccgacc aagggcgcga cggccaacag ttcgggcaca ttgggctcct
     1081 ccatgaagcc atagtgggcg atgatgcgct ggaaccccgc agcgagcttc gtgacttcca
     1141 agcgtttagc ggcatctact accggaactt cgtcggtgag aatggtgaga atgatgttgc
     1201 gctggtggat gactttgttg tgcttgaggt tgtggagcag cgcgattgga gtgccttcgg
     1261 gattggcggt cgtgaacaac gcagtgcccg gcacgcgcgg cactgcccga ttccgttcaa
     1321 tgtcgtccag gaacatttcc agcggcatgg aagccgggcg gagcttgttc cacaccagca
     1381 ggcgaccctt tttccaggtc gccatgagaa ggaagatcgc cagacccatc gcgagcggaa
     1441 accagccacc ctttagcact ttgagcaagt tcgccgcgaa gaaggcagtt tccaccacca
     1501 ggaacacgct acaggccgcc gccgctcgcc acgcgctcca gccccacacc cggcgcgcgg
     1561 cgaagaagaa caagacggtg gtcgaaagca tcgtcagcgt caccgcgatg ccgtaggccg
     1621 aggccatgtt gctggaggaa cggaagccga tgacgagccc gatgcacgcc aacatcagcg
     1681 cccagttcac atggggcagg taaatctggc cgcgctccat cgaggaagtg tgcttgacgg
     1741 ccatgcgcgg gagatagccg agttggatcg cttgaatggt taatgaatac gcccctgaaa
     1801 tcagggcctg ggaggcgatg acagtcgccg cggtagccag ccctaccagc agatagaggc
     1861 cccagtgggg cgccagcagg tagaatggat tctcaatact tgtcgggtta tgcaatatca
     1921 gggccccctg accaaggtag ttgagaaaca accccgggag gaccagtcca aaccaggcga
     1981 tgcgaatggg ccgggctcca aagtgcccca cgtccgcata gagggcctcg gcgccggtga
     2041 cagccaaaaa cacggagcct aagacgacga aacccgtcca cccgctgcgg aagaggaact
     2101 ccagccctag taacgggtcg agcgaccaga gaacgcttgg cgacatcagg ataccacgga
     2161 agcccagtag cccaagcgag acaacccaca acaccatgat tggcccgaat accgcgccga
     2221 ctctcgccgt gccgcggctc tgagcggtga ataaaccgat aagggtaacc acagtaatcg
     2281 gaatgacata atgctcgaat gcaggcgtcg ccacattgag gccctcgact gcgctcagca
     2341 cggagattgc cggcgtgatc attccgtcgc cgtaaagcaa ggcggctccg aaaacaccca
     2401 ggccgattag caaggcgcgg cgtcggctag cgccaggaga ttgtctgggg aaagccaggg
     2461 ccatcaacgc caggatgccc ccttcgcccc ggttggtcgc acgcagcacg atggccaagt
     2521 acttcagcga gacaatgagc aggagggacc aaaaaatcag cgacaagatg cccagcacat
     2581 tggggcgcgt gggtggcaaa gggtgcgaac cggcaaagca ctcgcgcaat gcgtagagcg
     2641 ggctggtgcc aatgtcacca t
//
LOCUS       DATCGS010000482         5645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_288040, whole genome shotgun sequence.
ACCESSION   DATCGS010000482 DATCGS010000000
VERSION     DATCGS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288040"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..975
                     /locus_tag="VIL39_09890"
     CDS             238..975
                     /locus_tag="VIL39_09890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY5481779.1"
                     /translation="MQLEWLFGQKQPAQPEDHWLRAGTRPVRLRFVCNRRARRYILRL
                     RSDGTARVTVPRGGALAEAKRFAERNVAWLEKQLLRQALRPLRPNTWPAGTETLFRGE
                     RVRLETDANGGSGVVRFGSEAVPIADANGDLRPAVERHLRQLAARELPPRVLELAALH
                     NLPVRRVTVRNQRSRWGSCSRRGTISLNWRLVQAPDFVRDYIVLHELAHLKEMNHSRR
                     YWGEVARLCPDFPAAECWLKQHSILLA"
     gene            complement(989..2020)
                     /gene="cydB"
                     /locus_tag="VIL39_09895"
     CDS             complement(989..2020)
                     /gene="cydB"
                     /locus_tag="VIL39_09895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY5481780.1"
                     /translation="MNYGSLDLNTVWFILIGVLFTGYAMLDGFDLGVGALHLFTRTDE
                     ERRTMLNAIGPVWDGNEVWLVTGGGALFAAFPNVYATVFSGFYLAFVLLLVALIFRAV
                     AIEFRSKQPMRWWRQMWDIGFSAGSLLSSLLIGVAMGNIAWGIPLDERGEFAGTFGSL
                     LGPYPLLLGITTVALFMMHGAIYALLKTDGPLHDKLRGWITHCIIFFIICYATTTMAT
                     LLYVPHMAARMRANPWLFSIALLNMLAIANIPREIHRRRDGRAFLSSCAAMIALMGLF
                     GLEMYPNLVLSNPMPANSLTIHNAASSHQTLGIMLTIALTGVPFVLAYTTGIYWVFRG
                     KVKLDKMSY"
     gene            complement(2032..3405)
                     /locus_tag="VIL39_09900"
     CDS             complement(2032..3405)
                     /locus_tag="VIL39_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY5481781.1"
                     /translation="MSVLTLSRFQFALTIGFHYLYPPLTIGLGVMLVIIEGLWLKTGE
                     PAYHQMARFWTKVFALTFAIGVATGIVMEFEFGTNWATYSRYVGDVFGSALAAEGIFA
                     FFLESGFLAVLVFGWSKVSRRVHFFATCMVCLGAHFSAIWIVVANSWMQTPAGFHIVG
                     EGMRARAEITDFWQMVFNPSSMDRLFHTLCGAWQAGAFLVVSVSAWYLLKSKYQDFAR
                     RSLRVGLTVGLAASLLQLVSGHSSAVGVARNQPVKLAAFEGLYETTSNAPLSLFGWVD
                     EKEEAVKGSLHIPGLLSFLAHNDTHQPVTGLREAAPKPEDRPPVNFSFQLFHLMAAIG
                     FGMILLASLGFLYFWRGSLFEKRWLLWLLVLSVLGPQLANQAGWFAAEVGRQPWIVYG
                     LLRTSQGLSAVVKADTVLASIILFSAVYLLLFTVFIYLLNDKIQHGPDEADLTPSGKL
                     ALPLTKH"
     gene            complement(3607..5328)
                     /locus_tag="VIL39_09905"
     CDS             complement(3607..5328)
                     /locus_tag="VIL39_09905"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5481782.1"
                     /translation="MRIGFKHPRLLPALSTLFILSLPLASPLSAATPAAKPNIVLILA
                     DDLGFSDLGCYGSEVTTPNLDKLASGGLRFTQFYTTPRCCPTRAALLTGLYPHQAGMG
                     AMMEDRGLPAYRGELSDRCITIAEELRLAGYHTLMAGKWHVCHIHFEGKKQLNFQSDT
                     PFWKSKSDWPLQRGFEEYYGAIHGVTSYYDPFSLVRGNTPIRAEGTNFYYTDAISDAA
                     VADIEKYAGGEKPFFLYVAYSAPHWPLQAPAEDIAKYRQRYMAGWDVIRTNRYRRQIE
                     LGLIAKTWPMSPRDERVPAWENAPHKEWQANRMATYAAMVERMDRGIGRIITRLKEKG
                     ADQNTLVLFFSDNGGCAEVIQPDWYDVPGRTRDGRPIRVGNGDPAVLAGPDDVWQSYG
                     VPWANVSDTPFRLYKHFVHEGGIATPFIAYWPAVIRQPGSVTRQLGHVTDLMATFVDI
                     AGARHPATYLGRAILPLEGKSLLPILQGQQRPDRSPIFWEHEGNRAMRLGQWKLVSLY
                     PNAWELYDMESDRTELHNLAAQQREKVEEFSGLYQQWAQRCGVLPPDQLPKPRPTVPD
                     KTKATAE"
BASE COUNT         1137 a         1715 c         1772 g         1021 t
ORIGIN      
        1 gttgggccgg cacatcggcc agcttatccg caagcccttc ccgcgctctc ccgtcgcttg
       61 aaagctacct tctttgagcg aaaccaaccg gtcagatgca gtccgcatga tggacggagc
      121 caacccccca gccgccgcag acgtttaaac cggcagaatg tcctcgctcg aaccagattc
      181 aactcgactt gcggcgattg tgttgccaag gccccgcccg gccacttaaa tcagagcatg
      241 caactcgaat ggctctttgg gcagaagcaa cccgcccaac ctgaggacca ctggctgcga
      301 gccggcacgc ggcccgtgcg tctgcggttc gtctgcaacc ggcgggcgcg gcgctacatc
      361 ctgcgcctcc gttcagacgg gaccgcccgg gtgaccgtcc ctcgcggcgg agcgctggcg
      421 gaagcaaagc ggtttgcgga gcggaacgtt gcctggctcg agaaacagct tctgcggcaa
      481 gcgctgcgac cgttgcggcc caatacctgg ccggcgggga cagaaactct cttccgggga
      541 gagcgggtgc ggctggaaac tgatgccaat ggggggagcg gagtcgttcg attcggcagt
      601 gaagccgtgc ccatcgcgga tgcgaacggc gacctgcgcc cggcggtcga gcggcatctc
      661 cggcagttgg cggcgcggga attgccgcca cgggtgctcg aactggccgc attgcacaac
      721 ctccccgtgc ggcgcgtgac cgtgcgcaac cagcgatccc gctggggctc ctgctcgcgg
      781 cgcggcacga tctccctcaa ctggcgtctg gtgcaggccc ccgacttcgt ccgcgattac
      841 attgtgctgc acgaactcgc gcacctcaaa gaaatgaacc actcccgacg gtactggggc
      901 gaggtggcgc ggctctgtcc cgatttcccg gcggccgaat gctggcttaa gcagcactca
      961 atcctgctgg cgtaagccta atcctgcgtc agtaactcat cttgtccagc ttcactttgc
     1021 cgcggaaaac ccaatagatg ccggtcgtgt aagccaacac aaacggcacc ccagtcaggg
     1081 cgatggtcag catgatgccc agcgtctggt gcgaagaggc tgcgttatga atggtcaggc
     1141 tgttggctgg catcgggttg ctcaagacca ggttcggata catctccagg ccgaacaggc
     1201 ccatcaacgc gatcattgcc gcgcaggaag atagaaaggc ccgcccgtcg cggcgccgat
     1261 ggatttcgcg gggaatgttc gcaatggcca gcatgttgag gagggcgatg ctgaacagcc
     1321 acgggttggc gcgcatgcgc gcggccatgt gcggcacgta gagcagcgtc gccatcgtgg
     1381 tcgtggcgta gcagataatg aaaaaaatga tgcaatgggt gatccagcca cgcagtttgt
     1441 catggagcgg gccgtcggtt ttcagcaggg cgtagatcgc cccgtgcatc atgaacagag
     1501 ccacggtggt gattcccagc agcagcgggt agggaccgag caggctcccg aacgtgccgg
     1561 cgaactcgcc gcgctcatcc agtggaatgc cccaggcgat gttgcccatg gccacgccaa
     1621 tgagcaggct ggacaggagg ctgccggcgc tgaagccgat gtcccacatc tggcgccacc
     1681 atcgcatggg ctgcttgctc cggaactcga tggccaccgc gcggaagatc agcgcgacga
     1741 gcaacagcac gaaggcgagg tagaagccgg agaatacggt ggcatagacg ttcggaaagg
     1801 cggcaaataa agcaccgcca cccgtgacca gccacacttc gttgccgtcc caaacggggc
     1861 cgatggcatt gagcatggtg cgccgctcct cgtcggtcct cgtgaacagg tgcagcgcgc
     1921 ccaccccgag gtcgaagccg tccaacatgg cgtagccggt gaacagcact ccaatgagga
     1981 tgaaccacac ggtattgaga tcgagcgagc cgtagttcat gattccccca ttcaatgctt
     2041 tgtcaaaggc agcgccagct tgcccgacgg tgtgaggtcg gcctcatcag ggccgtgctg
     2101 gattttgtcg ttcagcaggt agatgaagac cgtaaagagg agcaggtaaa ccgcgctgaa
     2161 caggataatg gaggccagca cggtgtcggc tttgaccact gccgagagcc cctgggaggt
     2221 gcgcaggagc ccatagacaa tccacggctg ccggcccact tcagcggcga accagccggc
     2281 ctggttggcc aattgcggcc cgagcaccga gagcaccagc agccagagca gccagcgctt
     2341 ttcaaagagc gacccgcgcc aaaaatagag gaagcccagc gaggcgagca atatcatgcc
     2401 gaaaccgatc gcggccatga ggtggaagag ctggaagctg aagttgaccg gcggacggtc
     2461 ttccggcttg ggtgcggctt cacggaggcc ggtgaccggc tggtgggtgt cgttgtgcgc
     2521 caggaagctc aacagcccgg ggatgtgcag gctgcctttg acggcctcct ccttttcgtc
     2581 cacccagcca aagaggctca agggtgcatt tgaggtggtc tcataaagcc cctcgaacgc
     2641 cgccagcttg acgggctggt tcctggccac tcccaccgcg ctgctgtggc cgctcaccag
     2701 ttgcaacagt gatgccgcca ggccgaccgt caggcccact cgcagggagc ggcgggcgaa
     2761 gtcctggtac ttgcttttca acagatacca ggcgctgaca ctcactacca gaaaggctcc
     2821 tgcctgccac gcgccgcaga gcgtatggaa tagccggtcc atcgaggagg ggttgaagac
     2881 catttgccag aagtcggtga tctccgcgcg cgcccgcatt ccctcgccca cgatgtggaa
     2941 accggccggt gtttgcatcc acgaattggc caccacgatc cagatcgcac tgaaatgggc
     3001 ccccaggcag accatgcaag tcgcaaagaa atgcacgcgc cggctgacct tgctccaacc
     3061 aaacacgagc acggccagga agcccgattc gaggaagaat gcaaagatgc cctcggctgc
     3121 caacgcactg ccgaacacgt cgccgacgta gcgcgagtag gttgcccagt tcgtgccaaa
     3181 ctcgaactcc atgacaatgc ccgtggccac gccaatcgca aacgtgagcg caaagacttt
     3241 ggtccaaaac cgggccatct ggtgatacgc cggttccccg gtcttcaacc acagtccctc
     3301 gataataacc agcataaccc ccaagccgat ggtcagcggc ggatacaggt aatgaaaccc
     3361 gatagtcagc gcgaactgga agcgggaaag agtcaggacg ctcattgttt ctccccatcc
     3421 ttgaaccagg tttccttcca ccgctggccc aggccggcgg ctccagcagg gatcgggtta
     3481 gtcatacacg ggcaaatagt aagggttgag ccggtccctt tgcaataagg aaccacggcg
     3541 ggtcaccagg acgggagcat ctcattttta gaacccgaac agcaattcaa caactcggtt
     3601 ggcggatcat tcggcagtcg ccttcgtttt gtcaggaacc gtcggtcggg gtttggggag
     3661 ttggtctgga ggcaggactc cgcagcgctg cgcccattgt tggtagaggc cagagaactc
     3721 ttccaccttc tcccgctgct gcgcggccag attgtgcagt tccgtgcggt cgctctccat
     3781 gtcataaagc tcccaagcgt tggggtacag tgaaaccagc ttccattggc ccagccgcat
     3841 ggcgcgattg ccttcgtgct cccagaagat cggcgaccga tccgggcgct gctggccttg
     3901 aagaatcggc agcaggcttt tgccttccag cggcaggata gcgcggccca agtaagtggc
     3961 gggatggcgc gcgccggcga tgtccacaaa ggtggccata aggtcggtga catgtcccag
     4021 ttggcgagtc acactgcctg gttgacgaat gaccgccggc caatacgcga tgaagggtgt
     4081 cgcgatacct ccctcatgga caaaatgctt gtagagccgg aagggcgtgt cgcttacgtt
     4141 agcccaaggc actccatagc tctgccaaac atcgtccggc ccggccagca cggctgggtc
     4201 gccatttccg acccggatgg gccggccatc gcgggtccgg cccggcacat cataccagtc
     4261 cggctggatg acttcggcgc acccgccgtt atcggagaag aagagcacca aagtgttctg
     4321 gtcggcgccc ttttccttca gcctggtgat tatgcgcccg atgccgcggt ccatgcgctc
     4381 gaccatcgcg gcgtacgtgg ccatccggtt ggcttgccat tctttgtggg gggcgttttc
     4441 ccaggccggc acgcgctcgt cccggggcga catcggccag gtcttggcga tgagacccag
     4501 ttcaatctgc cgccggtagc ggttggttcg aatcacgtcc cagccggcca tatatctctg
     4561 ccggtatttg gcaatgtcct cggcgggggc ttgcagcggc cagtgcggag cgctataggc
     4621 gacgtagagg aagaagggtt tttccccgcc ggcatatttc tcgatgtcgg ccaccgccgc
     4681 gtcgctgata gcgtcggtgt aatagaaatt ggtgccctcg gccctgatgg gggtgttgcc
     4741 gcggacgagg gagaaaggat catagtagct ggtgaccccg tgaatcgcgc cgtagtattc
     4801 ctcgaagcct cgttggagcg gccaatcgga tttgcttttc cagaagggcg tgtcggactg
     4861 gaagttgagt tgtttcttgc cctcaaagtg gatgtggcac acatgccatt tgccggccat
     4921 caaagtgtga tagcccgcga ggcgcagctc ttcggcgata gtaatgcatc ggtcgctgag
     4981 ctcgccgcgg tatgcgggca ggccacggtc ctccatcatg gcgcccatgc cggcctggtg
     5041 aggataaagg cccgtcagca atgccgcacg cgtcggacag cagcgcggtg tggtgtaaaa
     5101 ttgggtgaac ctgaggccgc cgctggccag tttgtccagg ttcggcgtgg tgacctccga
     5161 accatagcaa cccagatccg agaagccgag gtcgtcagca agaatgagga cgatattcgg
     5221 cttggcggct ggcgtggccg ccgacaaagg cgaagccagc ggcaggctca aaataaacaa
     5281 ggtgctcagc gcaggcagca accgcggatg tttgaagccg attcgcatat atctgagccg
     5341 gagtctaaga caaccaccaa gctgatggga aatggttttt gtaactgttc aggtagtggg
     5401 gcgggcgtct cgcctgccgc accgggcgtc tcgcccgcgt tgccaaaacc gagggcgaga
     5461 cgccctcctg acaggcgaga cgcccgcccc actatctgaa cagttacgtt ttgctttgca
     5521 gccggggccc aggcggggta agcaaccggc tgcgatgaac cgtctattgc ctctttcgct
     5581 cgcggtattg ctggtcgcgg ggtgcagttc gctcaggact accgtgcgcc acaccggccc
     5641 gtgga
//
LOCUS       DATCGS010000483         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_140895, whole genome shotgun sequence.
ACCESSION   DATCGS010000483 DATCGS010000000
VERSION     DATCGS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140895"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            618..3056
                     /locus_tag="VIL39_09910"
     CDS             618..3056
                     /locus_tag="VIL39_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY5481783.1"
                     /translation="MVHFNEGRIIHSQPGQPWGQEIVQVSPLDTATASQTNSYRVSST
                     NDSAYAQAQSPLRVGRKSKGTDFAWSNVSPYCSREHWLYLTLPQPMQRGKTYTIDTAA
                     LATNGRYWALTFDETRARSEAVHVNLLGYVPGAPQKYAYAFHWMGNLGSLDLSGYQGR
                     SFRLIDQQTGATAFTGQLAFRKPATQAETGQAADTPNGNFLGADVYECDFSSFTRSGT
                     YVVAVDGIGCSFSFQVKSDVYREAFRTVIRGLYHNRSGIELKPPYTTFARPAPHNPSL
                     TPGFTNKLLYTSLRFTEWGSEGGDAATLLAKVKGPIQSAGWYQDAGDWDSYYSHLSIA
                     QMLLFAYEMAPRNFSDGELDLPEGINLVPDVLDEAAWLPRFLYRLRHELMAKHYGSGG
                     VGLRICGDAFGSDNPNNILAGSWQDTNRTWVASGEDPWSTYRYAGACAHLAYCLSLTG
                     APDPEGIDWAGEATAAYAWAATNTLTGDNTNTVPGPLAHARAYAAAALFRITGVKAYE
                     NQFSNDEASVTSTTVLAQGDAAYPVMLYVLGGGAANYNSTLLNRMRSALLATADEYAI
                     TTSSKRSLRWGGNFWMPMLVGQQTAPAVLEVAAGYAVAKTTNPAQAQQYLGILYTTCD
                     YFLGGNSLNMTWATGLGVRHPNQVFHIDSWCLGYHPGMIPYGPWRTETANPTWVTDHD
                     WPNLTVYPAITNWPGNERWYDNRWSPMNSEFTISQTIAPSAAIFGLLCAPGLAAPVTP
                     PAVPITITNTLTNALLLRWPSESTGGLVLQQSTNLSGANWIAVPQFPADDGTNKSVLA
                     PPASPIQVFRLKWP"
     gene            complement(3043..3195)
                     /locus_tag="VIL39_09915"
     CDS             complement(3043..3195)
                     /locus_tag="VIL39_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481784.1"
                     /translation="MSRRKETRRSGGAVQCKNQADPNVSDAKNYGNNQYGTLTAGDKD
                     LIVKAT"
BASE COUNT          624 a         1150 c          948 g          671 t
ORIGIN      
        1 acccctcgcg caaaccgcct acagcagtcg gcgccatctc tcggaactga gaatgagcgc
       61 cggctgccgt cggcggctac gcacgatttc ggcccggatg gtgtcccagg tcaggtcctc
      121 cgtgccgtcc acatcgccgc cggtggcggc gagcctggcc ggctacggca gcgtgccgat
      181 tgtgctccag gttacatttc ggacatcatt ggcgcggcgc cagtggggcc gaacgctgac
      241 tttgccagcc ccggttccgt cctcgacgcc ggggtcaggg gaaactatcc tgcttcggtt
      301 catgcgaaac atcatcttcc acggccctgg tggatgccgc gcacagcagg cacatttgat
      361 gcctcgacaa actcctgccg gggcgtaatg ttccttgcat gagcgaagcg ctacagtatc
      421 tcaccgacaa ctcccgcttg tcttgtcact agtttggatg acatgaccgc ttaccgcttc
      481 tcatgctagc atcgttgccg gcattgagtc ttgttcacac aactgcggcc cggcttttgc
      541 ttctgctgct gtgcgccccc ctcttggccg gggcggcaac aatcgcggaa gtgctcccct
      601 tgaccgaccg gatagtaatg gtccacttca atgagggccg aattatccat tcccaaccag
      661 gtcaaccctg gggccaggaa atcgtgcagg tcagcccctt ggacaccgcc acggcttccc
      721 agaccaatag ctatcgggtt tccagcacca acgactccgc ctacgctcag gcccagtctc
      781 cactgcgggt ggggcgcaag agcaagggca cagactttgc ctggagcaac gtcagccctt
      841 actgcagccg cgagcactgg ctttatctga cgctgcctca gcccatgcag cgcggcaaga
      901 cctacacgat tgacaccgcc gccctcgcca ccaacgggag gtattgggcg ctgacgttcg
      961 acgaaacgcg cgcccgctct gaagcggtgc atgtcaacct gctggggtac gtccccggcg
     1021 cgccccagaa atacgcgtac gcgtttcact ggatggggaa cctgggttcg ctggatctct
     1081 cgggctacca gggacgatca tttcgtctga ttgaccagca aaccggggca acggcgttca
     1141 ccggccaact cgccttccgc aagcccgcca cccaggcgga aaccggccag gccgccgaca
     1201 ccccgaacgg caatttccta ggagccgacg tctatgagtg cgatttttca tccttcaccc
     1261 ggtcggggac atatgtggtc gcggtggatg gcatcgggtg ctctttttcg ttccaggtga
     1321 aatcggacgt gtatcgcgag gccttccgca cggtcatccg cggcctgtat cataatcgca
     1381 gcgggatcga gctcaagcca ccgtacacca cttttgcccg accggcgccg cacaacccga
     1441 gcctcacacc cggcttcacc aacaagctgc tctatacctc cttgcgtttc acggagtggg
     1501 gctcggaagg gggggatgcc gcgacgttgc tggccaaggt caaggggccg attcaatccg
     1561 ccggttggta ccaggatgcg ggcgattggg acagctacta ttcgcacctg agcatcgcgc
     1621 agatgctgct cttcgcctac gagatggcgc cgcgcaattt cagcgatggc gaattggacc
     1681 tgcccgaagg catcaacctc gtgccggacg tgctcgacga agcggcctgg ctgcctcgat
     1741 ttctctaccg cttgcgccac gaactgatgg ccaagcacta tggcagcggc ggcgttgggc
     1801 tgcgcatttg cggggatgcc ttcggctcgg ataatccgaa caacatcctt gccggttcct
     1861 ggcaggacac caaccgcacc tgggtcgcgt ctggagaaga tccctggtcc acctaccgtt
     1921 atgcaggggc ctgcgcgcac ctggcttatt gcctgagtct gaccggggcg ccggacccgg
     1981 agggaattga ctgggccggc gaggccaccg ccgcgtatgc ctgggccgca accaatacac
     2041 tgaccggcga taacaccaat accgtccccg gccccctggc ccatgcgcgc gcctacgccg
     2101 ccgcggcgct gtttcgcatc accggggtca aggcttatga aaaccagttc agcaatgatg
     2161 aagccagcgt cacttccacg accgttctcg cgcaaggtga cgccgcctac ccggtgatgc
     2221 tctacgtcct gggcggtggc gcggccaatt acaactcgac tctgctcaac cggatgcgct
     2281 cggccctgct ggcgacggcc gatgaatacg ctattaccac ttcgtccaaa cgctcactgc
     2341 gctggggcgg caacttctgg atgccgatgc tggtcggcca gcaaaccgcg ccggccgtgc
     2401 tcgaagtcgc cgccggctat gccgtggcaa aaaccactaa tcccgcccaa gcccagcaat
     2461 acctcgggat tctttacacc acctgcgatt acttcctggg cgggaactcg ctcaacatga
     2521 cctgggcgac cggcctgggg gtgcggcacc cgaaccaggt gtttcacatt gattcgtggt
     2581 gcctgggcta tcatccgggc atgattcctt atggcccctg gcgcaccgaa accgccaacc
     2641 ccacctgggt gaccgaccac gattggccga atctgaccgt ctatccggcc atcaccaact
     2701 ggcctggcaa cgagcgctgg tacgacaacc gttggtctcc catgaacagc gagtttacca
     2761 tcagtcaaac catcgccccc tccgcggcga ttttcggcct gttgtgcgct cccggcctgg
     2821 cggcccccgt cactccgcca gccgtgccca ttaccatcac caacaccctg acaaacgccc
     2881 ttttgctccg ttggccctca gaatccaccg gcggtcttgt gcttcaacaa agcaccaatc
     2941 tcagcggcgc caattggatt gctgtccccc aattccctgc cgacgacgga accaataaga
     3001 gcgtgctcgc gcctcccgcc tcaccaatcc aggtgttccg cctcaagtgg ccttaacgat
     3061 caggtccttg tctcccgccg tgagagtgcc atactggtta ttgccgtagt tcttagcgtc
     3121 gctcacgttc ggatccgcct gatttttgca ttgaaccgcg ccacccgatc ggcgagtctc
     3181 cttgcgtctg ctcatgcttg cggctcgttt ccatggatta atgaaagcaa ctattagcgt
     3241 ggttctcctg gccatgtgcc tgggtgccct tggccgtttc gaatgctcag ccggccaggc
     3301 cgccgagatg attgagcttt ggccggccgg agcgccggcc acaaacgaca atatcgccgc
     3361 ggagcatgat accaccaaac ccacggactc cct
//
LOCUS       DATCGS010000484         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_537283, whole genome shotgun sequence.
ACCESSION   DATCGS010000484 DATCGS010000000
VERSION     DATCGS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537283"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..465)
                     /locus_tag="VIL39_09920"
     CDS             complement(61..465)
                     /locus_tag="VIL39_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481785.1"
                     /translation="MKKLLTLAFWALFVSASGIAEAGTLFSGPVIFVTPQVLGFGPVA
                     GTTVVTNTLVVENMGKGTLIGTATVAAPFKILSGGAYSLRGSEAQIVTVIYVPAGATS
                     DTQTVTFTGGGGAKATVTVKPIESAPKKSKQK"
     gene            complement(717..1736)
                     /gene="ruvB"
                     /locus_tag="VIL39_09925"
     CDS             complement(717..1736)
                     /gene="ruvB"
                     /locus_tag="VIL39_09925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY5481786.1"
                     /translation="MAKDKIADVLAKPDAALEMTLRPSLFSEFTGQLKVKERLEITVA
                     AARQRKEAIDHVLFSGPPGLGKTTLAHILAKAMGANLKSTSGPTIEKAGDLAGLLTNL
                     EEGDVLFIDEIHRLQKTIEEYLYPAMEDFKLDIIIDQGPNARSVRLNLPRFTLIGATT
                     RSGLLTAPLLTRFPIRERLDYYQADQLQQIVVRSARLLNVETEEKGAWEIARRSRGTP
                     RIANNLLRRVRDYAQVRHEGRITADVADKALAMLEIDENGLDEMDKRILETVIVKFGG
                     GPVGISSIAVAVGEEPDTLEEVYEPYLIMEGYLKRTPQGRVATELSYKKLGLQGDPEK
                     QARLL"
     gene            1883..2692
                     /locus_tag="VIL39_09930"
     CDS             1883..2692
                     /locus_tag="VIL39_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481787.1"
                     /translation="MRALRSGWRGFTLIELLVVIAIIAILAALLLPALAKAKVAAKKV
                     QCINNEKQLATTWMLYASDNADWLPANGQNNVEPPSPSLKLWVQGCMAYDEDRTNTTY
                     ILDTRYAQFASYLQTLKVYLCPTDRPNVLVNGRSLPRIRSYALNAYVGARTDFWDNRL
                     AANFTMFRKHSEISSKLPTGTLTFIDVYADSLCWPYFGVYMTQDSFFNFPNSSHNRGG
                     VVSFADGHTEYHRWRDQRTITAYSADYHQHRDSSPNNPDLVWLRQRATVPK"
BASE COUNT          545 a          770 c          830 g          597 t
ORIGIN      
        1 caaggacgtc gcgcaggcgg ccaagttcat taagctctaa tacacgacct ggggcgcgct
       61 ctacttttgc ttggacttct tcggcgcaga ttcaatgggc ttgacggtta cggtggcctt
      121 tgctccccca cccccggtga atgtgacggt ctgagtatcg gaagtggcgc cagccggcac
      181 ataaatcacc gtaacaatct gcgcttcgct tcccctcaac gagtaagctc cgccagagag
      241 gatcttgaag ggagccgcaa cggtggctgt tccgatgagt gtccctttgc ccatattctc
      301 cacaaccagg gtgtttgtta cgacagtcgt gccggccaca gggccaaaac ccaagacctg
      361 cggcgtaacg aaaatcacgg ggccgctaaa cagagtgccc gcttccgcga tgcctgaggc
      421 actcacaaag agagcccaga aagcaagagt caatagtttc ttcattttgt ttaagatcac
      481 cgcataacgg ccttaggcat gcccttgaac tctatcaccc tgctccggcg cctaagccgg
      541 agcgccagac aaaccgcctc gggtttttgc tccgttcacg aatgggcctg ccgtttgccg
      601 tatttcagac ataatactaa cgttcttcta ctgctacaac aataaccttg ggtgccagcg
      661 cgatccaggt gtgacaaccg gacaagcatg gtttcttgta ttccacatca cagaatctag
      721 aggagcctcg cttgcttctc agggtccccc tgcaagccga gtttcttgta actgagctcg
      781 gtggcgacgc ggccttgagg ggtgcgcttg aggtagcctt ccatgatgag gtaaggctca
      841 tagacttctt cgagggtatc gggttcctcg cccactgcca ccgctatcga gctgatgccg
      901 acggggccgc cgccgaactt aacgatcacg gtctcgagga tgcgcttgtc catttcgtcg
      961 aggccgttct cgtcaatctc cagcatcgcg agggctttgt cggcaacatc cgccgtgatg
     1021 cggccttcgt gcctcacctg ggcgtaatcg cgcacgcggc gaagcaggtt gttggcaatg
     1081 cgcggtgtgc cgcggctgcg gcgggcgatt tcccacgcgc ccttttcctc cgtctcgacg
     1141 tttaacagcc gggccgagcg gacgacgatc tgctgaagtt ggtcggcctg gtaatagtcg
     1201 aggcgctcgc ggatgggaaa acgggtgagc aacggcgccg tgagcaggcc gctgcgggtg
     1261 gtggcgccga tgagcgtaaa gcggggtaaa ttaaggcgga cactgcgggc gttgggcccc
     1321 tggtcaataa tgatgtcgag cttgaagtcc tccatggccg ggtagaggta ttcctcgatg
     1381 gtcttctgga ggcggtggat ctcgtcaatg aatagaacgt cgccctcctc gaggttggtc
     1441 aacaggccgg cgaggtcacc ggccttttcg atagtggggc cgctggtgct cttgaggttg
     1501 gcgcccatcg ccttggcgag gatgtgggcg agggtagttt tgccgaggcc gggtgggccg
     1561 ctaaagagaa cgtgatcaat ggcttccttg cgctgcctgg ccgcggcgac ggtgatttca
     1621 aggcgctctt tgactttgag ctgccccgtg aattcagaga acagggaggg gcgcagcgtc
     1681 atctccaacg cggcatcggg cttcgccagg acgtctgcaa tcttgtcttt ggccatcgct
     1741 tgaaggatgg gcaaaggccg ggcgggcgca aggcggaaag tcgcgcggcg gaaagtcgcg
     1801 cggcgggcag aactcagggt tttcgtgctt gcaatgcggt tgtggcagag ctatcaagag
     1861 taagtcgtcc cgagcgagtt ttatgcgcgc cttaagaagc gggtggcggg gttttacgct
     1921 catcgagttg ctggtggtca ttgccatcat cgccattctc gcagcgttgc tgctgcctgc
     1981 gctggcaaag gcgaaggtgg cggcgaaaaa ggtccagtgc atcaacaacg aaaagcagtt
     2041 ggcgaccacg tggatgctgt atgcgtcgga taacgctgat tggctgcccg ccaacgggca
     2101 aaacaatgtg gaaccgccta gcccctcctt gaagctgtgg gtgcaagggt gcatggctta
     2161 tgatgaggat cgaaccaaca cgacgtatat cctggacacc aggtatgctc agtttgcgag
     2221 ttacctccaa acgctcaagg tgtacttgtg cccgaccgac cggcctaacg tgctggtcaa
     2281 cggccgatcg cttcccagga tccgcagtta cgccttgaac gcctacgtcg gcgctcggac
     2341 cgatttttgg gacaaccgcc tggccgccaa tttcacgatg ttccggaagc attctgaaat
     2401 ctcttcgaaa ttgcccacag ggacgctgac ctttatcgac gtatatgccg acagtctctg
     2461 ctggccttac ttcggggtgt acatgacgca ggatagcttc ttcaactttc caaatagctc
     2521 ccataatcgc ggcggggtgg tgagcttcgc tgacgggcat acggagtatc accgctggcg
     2581 ggatcagcgg acaatcacag cttattcggc ggattatcat cagcaccggg attcctctcc
     2641 caacaacccc gacctcgttt ggctgcggca gcgggccacg gttcccaagt aattcaccga
     2701 cagggagatc cgcacgccgt cgcggatatg gccggttgga aa
//
LOCUS       DATCGS010000485         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_91085, whole genome shotgun sequence.
ACCESSION   DATCGS010000485 DATCGS010000000
VERSION     DATCGS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91085"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="VIL39_09935"
     CDS             <1..2039
                     /locus_tag="VIL39_09935"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY5481788.1"
                     /translation="AANNHSVAFYLQLAAFAEFAGDEARLAECRRQFKEVFVPKQMAA
                     DGSFPAELKRTKPYGYSIFQLDNMVTLCQLLSTETDNLWSFALPDGRGIRKAVAFLYP
                     YLADKSTWPRQPDIQAWNGWPARQPALLFAGLAFGEQKYLDLWQKLPADPVDPEVRRN
                     IAITQPLLWLKPVPQAKAVASGASASAAERPPQRGELRTLSAPPTPRINGPSIYGVRP
                     GSPFLYHIPATGERPMEFSVEGLPAGLQVAADTGEISGVLKEPGRHLVTLRARNAKGT
                     AGKKFRIVAGEAITLTPPMGWNSWNCWGSKVDAEKVLKSARGLVASGLINHGWTYMNI
                     DDAWQGKRGGPFHAIQGNEKFPDMKDLCDTVHQMGLKIGIYSTPWVTSYATYIGGSAE
                     NPEGTWSSPTIPKRGNVNKKILPWAIGKYHFATNDASQWAAWGIDYLKYDWNPNELPE
                     TQEMYDALRKSGRDVIFSLSNNSPFTNAPALSKIANCWRTTGDIRDNWDSMSKKGFGE
                     DKWERFAAPGHWNDPDMLVVGRVGWGSPHPTKLTPDEQYTHISLWCLLSAPLLLGCDL
                     DQLDDFTLNLLTNDEVLAVNQDALGKQALCVARDGDLRVYTKDLEDGSKAVGLFNLGA
                     DTASVTVKWSDLRLSGRQSVRDLWRQKDVGAFGAQFQLPVAGHGAELVKLTPAQ"
BASE COUNT          427 a          711 c          612 g          381 t
ORIGIN      
        1 ctgcggccaa caatcattcc gtcgccttct acctgcagct cgcggccttt gccgagttcg
       61 ccggcgacga agcccggctg gccgaatgcc gccgccagtt caaggaagtg ttcgtcccga
      121 aacaaatggc ggccgacggc agcttccctg cggaactcaa gcggaccaag ccgtacggct
      181 actcgatctt ccagctcgat aacatggtca cgctttgcca gttgctctcc accgagaccg
      241 acaatctctg gagcttcgcg ctgcccgatg gccgcggcat ccgcaaggcc gttgcattcc
      301 tctacccgta tctggccgac aaatccacgt ggccccgcca accggacatc caggcctgga
      361 acggctggcc ggcccgacag cccgccttgc tctttgccgg tctcgccttc ggcgagcaga
      421 agtatctcga tctgtggcag aagctccccg ccgacccggt tgacccggag gtccgccgca
      481 acatcgccat cacccaaccg ctgttgtggt tgaagccagt accccaagcc aaagcggtag
      541 cttccggggc ctcggcctcc gctgcggaac ggccccccca acggggcgag ctccgcacgc
      601 tcagcgcacc gcccacaccg cggatcaacg gcccgtccat ctacggtgtg cggcccggct
      661 cgccgttcct gtatcacatc cctgctaccg gtgagcggcc gatggagttc tcggtcgaag
      721 gtctcccggc tggtttgcag gtcgccgcgg atacgggcga gattagcggc gtgttgaagg
      781 aacccggccg gcatctggtc acgttgcgcg ccaggaatgc caagggcact gccgggaaga
      841 agttcagaat cgtggcaggt gaggccatca ccttgacgcc gcccatgggc tggaacagtt
      901 ggaattgttg gggcagcaaa gtggatgcgg agaaagtcct caagagcgcc cgtggcctgg
      961 tcgcctcggg cctgatcaac cacggctgga cttacatgaa catagacgat gcctggcagg
     1021 gcaaacgcgg cggccccttc cacgccatcc aggggaatga gaagttcccg gacatgaagg
     1081 acctctgtga cacggttcac cagatgggct taaagattgg catctattcg acgccgtggg
     1141 ttacctccta cgccacctac atcggcggca gcgccgaaaa tcccgaaggc acctggtctt
     1201 ccccgacgat ccccaagaga ggcaatgtga acaaaaagat tctcccttgg gccatcggca
     1261 aataccactt cgccaccaac gatgccagcc aatgggccgc ctgggggatt gattacctga
     1321 aatacgattg gaatccgaat gagctgccgg agacgcagga gatgtatgat gccctccgca
     1381 agagcggccg cgatgtcatc ttcagcctct ccaataactc tcccttcacc aacgccccgg
     1441 ccctgtcgaa gatcgccaac tgctggcgca ccaccggcga catacgagac aactgggatt
     1501 ccatgagcaa gaaaggcttc ggcgaggaca aatgggagcg gttcgccgcc ccgggccatt
     1561 ggaatgaccc cgacatgctc gtcgtcggcc gcgtcggctg gggcagcccg catccgacca
     1621 agctgacacc ggacgaacag tacacgcaca ttagcctgtg gtgcctgctc tccgccccgc
     1681 tgctgctcgg ctgcgatctg gatcaactcg acgacttcac gctcaacctg ttgacgaatg
     1741 acgaagtcct ggccgtaaac caggacgcgc tcggcaagca ggccctttgc gtggccaggg
     1801 acggggacct tcgagtctat accaaagacc ttgaagacgg ttccaaagcc gttggcctct
     1861 tcaacctggg cgctgacacc gcctccgtga cggtcaaatg gtctgacctg cgcctgtccg
     1921 gcagacagtc ggttcgcgac ctctggcgcc aaaaggacgt tggcgcattc ggcgctcaat
     1981 tccaactccc cgtcgccggc catggcgccg aactggtaaa gctcacgccc gcgcaataga
     2041 ttatgaagac cagatttctt gtgggtctcg cggccatcct ggccatgcgc aattccccgt
     2101 gactgggctt cacttgggag gaatcctcct c
//
LOCUS       DATCGS010000486         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_734723, whole genome shotgun sequence.
ACCESSION   DATCGS010000486 DATCGS010000000
VERSION     DATCGS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734723"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1552
                     /locus_tag="VIL39_09940"
     CDS             122..1552
                     /locus_tag="VIL39_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY5481789.1"
                     /translation="MITTTSTPPAASHTCAGFNATYIWMISAVAAMGGLLFGYDWIVI
                     SGTDLFYEAHFHLNSSLQVGWAKSSALLGCLIGALVSGALSDRFGRKRLLALSALLFA
                     VSSVATGLAGSFAVFVLWRIAGGAAIGLASNLSPMYIAEVAPAQNRGRLVSLNQLTIV
                     IGILLAQFVNWRIGSLHPLPDQPTPDQIAASWSGQTGWRWMFGVTATPSLLFLIGMFF
                     VPESPRWLARNGKLEQARGILAKIGGGDYAKAALAEIQATLVNETQKVNFRDLLEPKL
                     KRVLFLGIALAVLQQWCGINVIFYYAKDVFKDSGFKVADILISIVFIGSVNLLATLVA
                     LKTVDRWGRRPLMLFGYAGLTVLFVVMGLCFALNTHGIHMLVLVLAAIACYAMSLAPI
                     TWVLIAEIFPNRIRGAAMSIAVTFLWIACFVLTETFPVLRETLGAAVTFWIYAGICAF
                     GFFFFKSKLPETKGKTLEQIEKELVD"
     gene            1620..>2618
                     /locus_tag="VIL39_09945"
     CDS             1620..>2618
                     /locus_tag="VIL39_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY5481790.1"
                     /translation="MTSRERILTACAHRQPDKLPVDFGGGFQTGIHVSLVYRLRQALG
                     LDRPGMPVKVVEIYQMLGETAPDLQAALGVDTVSLHGTGTMFGFPQTEFKEWQLADGT
                     PVLVPKDFNTQYEPNGDLLQWPAADHSVPPSGRMPAGGHFFDAIIRQEPLDETKLNPA
                     DNTEEFQPVPDAELEHFRRLAERLATTTDKALFCTFGGLTFGDIALVPATFLRRPKGI
                     RDVEEWYISIVSRPDYLKAVFERQAEVAIENLERLHTAIGDRAAIIQTNGADFGTQNG
                     PFLSPAKYRDLFLPYQKRINGWIHRHTPWKTFMHCCGGIAPLLDAVVEAEFDILNPV"
BASE COUNT          457 a          887 c          745 g          529 t
ORIGIN      
        1 tccccgcccc ttcatgaatt ggccctggca tgaccggaac gcaaacccgt tctcattgca
       61 gggagaagca agaagaagtt attttcttcc agatcctctc ctgctaagct gcgagtcaac
      121 gatgatcaca acaaccagca cgccccccgc agcatctcat acctgcgccg gctttaacgc
      181 aacttacatc tggatgatct cggccgtcgc cgccatgggc gggctgctct tcggctatga
      241 ctggatcgtg atcagcggca ccgacctgtt ttacgaggcg cactttcatc tgaactcctc
      301 cttgcaggtc ggctgggcca agagcagcgc gttgctgggc tgcctgatcg gcgccctggt
      361 ttcgggggcg ctcagcgacc gcttcggccg caaacggctg ctggccttat ccgccctgct
      421 gttcgccgtg tcttcggtgg cgaccggcct ggcgggttcc ttcgccgtgt ttgtcctctg
      481 gcggattgcc ggcggcgcgg ccattggtct ggcctcgaac ctgtcaccca tgtatatcgc
      541 cgaggtggcc cccgcccaaa accgaggccg gctagtctcg ctgaaccagc ttaccatcgt
      601 cattggcatc ctgctggccc agttcgtcaa ctggcgcatc gggagccttc atcccctgcc
      661 ggaccaaccc acccctgacc agatcgccgc ctcctggagc gggcagacgg gctggcgctg
      721 gatgtttggc gtcaccgcca cgccctccct cctgtttctg atcggcatgt tcttcgtgcc
      781 ggagagcccg cgctggctgg ccaggaatgg caaactggag caggcccgcg gcatcctcgc
      841 gaagatcggc ggcggcgatt acgccaaggc cgccctggct gaaatccagg ccacgctcgt
      901 caacgaaacc cagaaggtca acttccgcga cctgcttgag ccaaagctga agcgcgtgct
      961 cttcctgggc atcgcgctgg ccgtcctgca acagtggtgc ggcatcaacg tgatctttta
     1021 ctatgccaaa gatgtcttca aggactcggg cttcaaggtg gcggacatcc tgatcagcat
     1081 tgtctttatc ggcagcgtga acctgctggc cacgctggtc gcgctcaaga ccgtggaccg
     1141 ctgggggcgc cgaccgctga tgctcttcgg ctacgctggg ctcacggtgc tcttcgtggt
     1201 catgggcctc tgcttcgctc tcaacaccca tggcattcac atgctggtgc tcgtcctggc
     1261 ggccatcgcc tgttacgcca tgtcgctggc cccgatcact tgggtgctga ttgccgaaat
     1321 cttccccaac cgcatccgcg gcgccgcgat gtccatcgcc gtgaccttcc tctggatcgc
     1381 ctgcttcgtg ctcaccgaga ccttccctgt gctccgggaa accctcggcg ccgccgtcac
     1441 tttctggatt tacgcgggga tctgtgcctt cggcttcttc ttcttcaaat ccaagctgcc
     1501 ggaaacgaag gggaagacgc tggagcagat cgaaaaggag ctagtggact gaagatgcag
     1561 atgactacgc ctgaattcgg atttcgcact ttggacttcg gactttggac tttcccctta
     1621 tgacctcacg cgaacgcatt ctcacggcct gcgcccatcg gcagccggat aaactccccg
     1681 ttgattttgg cggcggcttc cagaccggca tccacgtcag cttggtttat aggctccggc
     1741 aggcgctcgg gctggatcgg cccggcatgc ccgtcaaggt ggtcgagatc taccagatgc
     1801 tcggcgaaac cgcccccgac ttgcaggccg cgctgggcgt tgatacggtc agcctccacg
     1861 gcaccgggac gatgttcggc ttcccgcaaa cagagttcaa ggaatggcag ctcgctgacg
     1921 gcacaccagt gctggtgccg aaggatttca acacgcagta cgagcccaac ggcgacctgc
     1981 tccaatggcc cgccgccgac cactccgtgc ctcccagcgg ccgcatgccc gccggcggcc
     2041 atttcttcga cgccatcatc cggcaggagc cgctggacga gacgaagctg aaccccgctg
     2101 acaatacaga ggaattccag cccgtccccg acgccgagct ggaacacttc cgccgcctcg
     2161 cggagcgcct cgccacgacc accgataagg cgttgttctg caccttcggc gggttgacct
     2221 tcggcgacat cgcgctggtg cccgccacat ttctacgccg ccccaaaggc atccgcgatg
     2281 tcgaggaatg gtatatcagc atcgtatcgc gcccggatta cctcaaagct gttttcgagc
     2341 ggcaggccga ggtggctatc gagaacctcg agcgcctgca cacggcgatc ggcgaccgcg
     2401 cggccatcat tcagaccaac ggcgccgatt tcggcacgca gaacgggcca ttcctgagtc
     2461 ccgcgaaata ccgcgatctg ttcctgccct accagaagcg catcaacggc tggattcacc
     2521 gccatacccc ttggaagacg ttcatgcatt gctgcggcgg catcgcgccg ctgctggacg
     2581 ccgtggtcga agccgagttc gacattctca accccgtg
//
LOCUS       DATCGS010000487         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_236596, whole genome shotgun sequence.
ACCESSION   DATCGS010000487 DATCGS010000000
VERSION     DATCGS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236596"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..995
                     /locus_tag="VIL39_09950"
     CDS             24..995
                     /locus_tag="VIL39_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY5481791.1"
                     /translation="MNKSHSLSRRTFIKTTGLAVSAGMLPSVSPLAAPTNKKWPVGCR
                     DLHLKLAGQPDSWSCMKALGAEGTEVNVELNLNCASLFHPQHKYTLATADGIQVLKDD
                     LASSGCRITAFMMSNHFDERLEQELESARGLAKAAQQLGVDIIRIDVVPRKLDGDQFL
                     PFAIDACKRLCAIAEGTPIRFGVENHGKITNNPQFLEKLFNGVGSPKLGLTLDCANFY
                     WWGHPVNDLYPIYEKFAPRVVHTHCKSIRYPEDKKNIRREIGWEYGKYNCPIYEGDLD
                     FKRIVGILRKAGYRGDLCVEDESLGKHPANEQADIMRKEIAMLKKLV"
     gene            1044..1337
                     /locus_tag="VIL39_09955"
     CDS             1044..1337
                     /locus_tag="VIL39_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481792.1"
                     /translation="MKAKLLITICVVAVSLGSVASSSATENSGPLEVTADVLVVRPAC
                     LVATVIGSAVFVVALPVAAISKSVKKTANTLVVKPANATFTRPLGDMGALVDD"
     gene            1409..2131
                     /locus_tag="VIL39_09960"
     CDS             1409..2131
                     /locus_tag="VIL39_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5481793.1"
                     /translation="MITEESFIDFKCPYCGDLVSFPQESAGFVQACPNCFESLIVPDD
                     GSEVGRQIPLPITTPKLVLRRLAAGDWRDLLELMSDEEFFRYQDGTPLDEDGILHWLE
                     SDPHVRLTTPDHPFFLGIQVQDGGKLIGYLSLSFTDPQRLQVTFNIGLNQNFHRKGYA
                     LEAVEAVLGFSFEGLKLHRVAGWCDSRNTAACRLLEKAGLRKEGEFVKNRWVHGEWTN
                     SIWYAALGEEYLQADTKVSEPS"
     gene            complement(2236..3096)
                     /locus_tag="VIL39_09965"
     CDS             complement(2236..3096)
                     /locus_tag="VIL39_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEY5481794.1"
                     /translation="MATIGKRNTLSIVRASAPGLYLDGGELGEILLPGRYIPADLKPK
                     DKLDVFIYRDSEDRLVATTETPLAMVGEFAYLKVISVNQNVGAFLDWGLAKDLLLPFR
                     EQHFPVRAGERVIVYVCLDVKTNRILATTRLNRHLNRDAPAYRSGQPVNLLITGRTPL
                     GYNAIVENAHRGLLYHDNLASPLATGQKLKGFVRTVRPGGQIDLSLDASGYQRVAALT
                     DQIVQALECNGGHLAFDDDSSPEAIRQTFGVSKKAFKQALGKLYKARRIRFQNPGIQL
                     LENASWAPGH"
     gene            3199..>4523
                     /locus_tag="VIL39_09970"
     CDS             3199..>4523
                     /locus_tag="VIL39_09970"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEY5481795.1"
                     /translation="MSVELTEALFSKAAGWEVMKRARAYLEQGEVLSSNWTPPLLRGV
                     VQGGEVSFRASMVIKGEIDIENLCTCREARQWGKICAHGVAVGLHWLKAQQPATNPAP
                     ARSALGGAQTRLPAKKPSTLQRDDTGEPAELFIILPPNLDQAIARGKVMLVFEAKWSG
                     GRCPLSALSKGRSFAFSQQDNAIIERLETLTNGETPALLQMETKDFAALLPALAEHPN
                     ITLGKANEVTVTKRPLKLPLRATLEANGEIVLALKDKTTTFVMVGDWVWQQQTFQPLG
                     LPAAAKDVLRAPVRVPRSQVPLFLSQHWPQLQAAGDAEANFKLDDFTLEPQVPRFLLE
                     LKGGLAQLGALLQCAYGPRIMTVGVTATDESVWLPDPAVPTRYSSRDLSAECAALARL
                     QRNGFSRPDAHGKMQLLGQNAVLNFFAREFTKLQREWSVTLDEHLENRTL"
BASE COUNT          935 a         1315 c         1391 g          882 t
ORIGIN      
        1 agggctgtaa accgcagact acgatgaata aatcacactc cctctcacga cgtaccttca
       61 tcaagacgac tggtctggcc gtctccgccg gaatgctgcc ctcggtcagc ccactggcag
      121 cgccgaccaa caagaaatgg ccggttggct gccgcgatct tcatctcaag ctcgccggcc
      181 agccggatag ctggtcctgc atgaaagcgc tgggcgcgga aggcaccgag gtcaacgtcg
      241 agttgaacct caactgcgcg agcctgtttc acccgcaaca caaatacacc ctggccaccg
      301 ccgacggtat ccaggtgctc aaggacgatc tcgccagcag cggctgccgc atcaccgcct
      361 tcatgatgtc caatcacttt gacgaacggc tggagcagga acttgaaagc gcgcgcggcc
      421 tagccaaagc cgcccaacaa ctcggggtgg acattatccg cattgacgtc gtgccgcgta
      481 aattggacgg cgaccagttc ctcccgttcg ccattgacgc ctgcaagcgg ctgtgcgcca
      541 tcgccgaggg cacgccgatc cgctttggcg tggagaacca cggcaagatc accaacaacc
      601 cccagttcct ggaaaaactg ttcaacggcg tcggctcccc gaagctcggg ctcaccctcg
      661 actgcgcgaa cttctattgg tggggacatc cggtcaacga cctctatccc atttacgaga
      721 agttcgcccc ccgcgtggtg catacccact gcaagagcat ccgctatccc gaggacaaga
      781 agaacatccg ccgcgagatc ggctgggaat acggcaaata caactgcccg atctacgagg
      841 gcgacctcga cttcaagcgc atcgtcggca ttctccgcaa agccggctac cgcggcgacc
      901 tatgtgtgga ggacgagtcg ctcgggaaac atcccgcgaa cgaacaggca gacatcatgc
      961 gcaaggaaat agcgatgctg aagaagctgg tgtaggcgct ttttcgttcg acaaggcaag
     1021 ccttgtcgct gaatgtaaga gaaatgaaag ccaagctctt aattactatc tgtgtggtgg
     1081 cggtcagcct gggcagcgtc gcttcgagtt ctgcgacaga gaacagcgga cctctggagg
     1141 tgacagccga tgtccttgtg gttcgcccgg cttgcctggt cgcgacggtg atcggttcgg
     1201 cggtctttgt ggtcgccctg ccggtggcgg cgatctccaa gagtgtgaag aagaccgcca
     1261 acacactggt ggtcaagccc gccaatgcca cattcacccg cccgttgggt gatatgggcg
     1321 ccttggttga cgactagccg cacggggttc gggaaaacct gcagaccgcg cctgcattcc
     1381 gccaggatct ggcgcacgca agagctatat gattacagaa gagagcttta ttgattttaa
     1441 atgcccctat tgcggcgacc tcgtgtcttt ccctcaggaa agcgctgggt tcgtccaggc
     1501 ctgcccgaat tgcttcgaga gccttatcgt gccagacgac ggcagcgagg tcggacgcca
     1561 gattccactc ccgatcacga cgcccaaact cgtgttaagg cgcctcgctg cgggtgattg
     1621 gagggatttg ctggaattga tgtcggatga ggagtttttc cgctaccagg acggcacgcc
     1681 cctggatgaa gacgggatat tgcactggct ggagagcgac cctcacgtca ggctgaccac
     1741 ccctgaccac ccgttttttc tggggatcca ggtccaggac ggcggcaagc tcatcggcta
     1801 tctgagcctg agctttactg atccccaacg cctccaggtc acgttcaaca tcggcctcaa
     1861 tcagaatttc caccggaaag gctacgctct ggaggcagtg gaggccgtgc tcggcttctc
     1921 cttcgagggt ctgaagctgc accgggtcgc cgggtggtgc gacagccgca acaccgccgc
     1981 gtgccggctc ttggaaaagg ccgggctcag aaaagaaggc gaattcgtga agaacaggtg
     2041 ggtacacgga gagtggacca actccatttg gtatgccgcc ttgggcgaag agtatctcca
     2101 ggccgacacc aaggtctcgg agcccagtta attgcggtga ccgagtttga gttgcgggtg
     2161 ggcaatctga cgaccgattg cagattgtcc acatgcctgg cagattctgg cgtctccttc
     2221 cagaatgctc cgctatcaat ggccgggagc ccaggaggcg ttctcgagca actgaattcc
     2281 cgggttctga aaacggatgc gccgcgcttt gtagagcttg cccaaagcct gcttaaaagc
     2341 tttcttgctg acgccaaatg tctgccgaat cgcttcgggc gagctgtcat cgtcgaaggc
     2401 caggtggccg ccgttacatt cgagggcttg gacgatttga tccgtcaggg ccgccacgcg
     2461 ctggtatcct gaggcgtcga ggctcagatc aatctgcccg ccggggcgga cggtgcggac
     2521 gaagcctttg agcttctggc cggtggcgag tggggacgcg aggttgtcat ggtagagcag
     2581 gccacggtgc gcgttctcca caatggcgtt gtaaccgagc ggggttctgc ccgtgatgag
     2641 gaggttgacc ggttgtccgc tgcggtaagc cggcgcgtcg cgattcaggt gtcggttcaa
     2701 tcgcgtggtg gccagaatgc ggttcgtctt cacgtcgaga cagacataga caatcacgcg
     2761 ctctccagcg cgcactggga aatgctgttc gcggaagggc aacaacaaat ccttggccag
     2821 gccccagtcg aggaacgctc cgacgttctg attcacgctg atgactttca gatacgcaaa
     2881 ctcgccaacc atcgcgagcg gcgtttcggt ggtggccacg agccggtcct cggaatcgcg
     2941 atagataaag acatccagtt tgtctttggg cttgaggtcc gctggaatat agcggccagg
     3001 caacaggatc tcgcccaact caccgccgtc gagatagagg ccgggggcgg acgctctcac
     3061 aatggagaga gtattgcgtt tgccgatggt ggccatgccc acagcgtaag cgcgaattgc
     3121 ctgatccggg agaaaaatcg gcagcctaat cccttgtgtc cttgaacggt ggcttatccc
     3181 gcacgcatat tgagttgaat gtcagttgaa ctcacagaag ccttgttcag taaagccgcc
     3241 ggttgggagg tgatgaaacg ggcgcgcgcc tatctggagc aaggcgaggt gctcagttcc
     3301 aactggacgc caccgctcct gcgcggggtg gtgcaagggg gcgaggtttc tttccgcgcc
     3361 tctatggtga tcaagggcga gattgacatc gagaatctct gcacctgtcg cgaggcgcgg
     3421 cagtggggaa agatttgcgc gcatggcgtc gcggtgggat tgcattggtt gaaggcccaa
     3481 caaccagcaa caaatccggc tccggctcgc tcggcactcg gcggagcgca aacaagactc
     3541 ccggcgaaga agccttccac gctgcaacgt gatgataccg gcgagccggc tgaactgttt
     3601 atcatcctgc cgccgaatct cgatcaggcc atcgcgcgcg gcaaggtgat gctggtgttc
     3661 gaagcgaagt ggagcggcgg tcggtgtccc ctgagcgcac tgtcgaaggg ccgctcgttt
     3721 gccttctcac agcaggacaa cgcgatcatc gagcgccttg aaacgctaac caacggtgag
     3781 acgcccgccc tcctgcaaat ggagacgaag gatttcgccg cgctgcttcc ggcgctggcg
     3841 gaacacccga atatcacact aggcaaggcc aacgaggtca cggttacaaa gaggccattg
     3901 aaactcccgt tgcgcgccac gctggaggcg aatggggaaa tcgttctggc attgaaggat
     3961 aagacgacga cgttcgtgat ggtcggcgat tgggtttggc aacagcagac gtttcagccg
     4021 ctcggtttgc cggccgccgc gaaggatgta ttgcgcgcgc cggtgcgagt gccgcgctcg
     4081 caggtgcccc tgttcctgag tcagcattgg ccgcagttgc aggcggcggg tgacgccgag
     4141 gcaaatttca agctggacga tttcacactc gaaccgcaag tgccgcgctt tctattggaa
     4201 ctcaaaggcg gcctggcgca gctcggcgcg ctgctgcaat gcgcctatgg cccgcgcatc
     4261 atgaccgtcg gcgtgacggc cactgatgaa agcgtgtggc tgcctgatcc ggcagtgccg
     4321 acgcgttatt cctcgcgcga cttaagcgcc gagtgtgcgg cgttggcccg gttgcagcgc
     4381 aacggctttt ccagaccgga cgcgcatggg aagatgcaac tcctcgggca gaatgcggtg
     4441 ctgaacttct tcgctcgcga attcacgaag ctgcaacgcg agtggagcgt gacactggac
     4501 gagcatttgg agaaccgtac gct
//
LOCUS       DATCGS010000488         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_537549, whole genome shotgun sequence.
ACCESSION   DATCGS010000488 DATCGS010000000
VERSION     DATCGS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537549"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VIL39_09975"
     CDS             complement(<1..404)
                     /locus_tag="VIL39_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481796.1"
                     /translation="MKKTATNLLLRLICALALWTTTGLQAQTPPVTAGLQLWLNADVG
                     VTTNASAQVTAWADQSGLGNNATQPTVTSSPIYAPNSLNGHATLRVTGTQYMEVPNAN
                     GIDNLLNDVTILGVVKYDNLGGYRGFVSKCTGG"
     gene            767..1642
                     /locus_tag="VIL39_09980"
     CDS             767..1642
                     /locus_tag="VIL39_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5481797.1"
                     /translation="MGESHLGPGTAYPPAGHPKGYAFNTPKGRLLDEYQVIYISSGSG
                     WFRSAASGRVRINAGSVLLLFPGVWHSFGPAAATGWNEHWVGFNGEMARRLVRHGFFT
                     PERPVLRAGEENKLLGLFSSLMEATRSNHPALQQIMAGTTECVLALLYSTQQSTLAGD
                     DPGLRVIHQAVVRIREAGETTLNMPALASELKVGYRWFRRAFAHHTGLSPHQYFLDIR
                     LTRARDLLAQTSLSIKDIAFRLGFEEPQYFCKLFHKKVGLTPGVWRAQAQTGNLTRTG
                     SKAASPTASNRAPSA"
     gene            1931..3334
                     /locus_tag="VIL39_09985"
     CDS             1931..3334
                     /locus_tag="VIL39_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HEY5481798.1"
                     /translation="MFGIGLEAYWPQFKGLKERLEGYLGIVQERLQRDEVTVVNLGLI
                     DSPPKALEAGHHFRQQDVDLIFLYVTTYALSSTVLPVVQRAKVPVIVLNLQPAAAIDY
                     ASFNRMGDRTAMTGEWLAHCSACPVPEIANVFNRARVNFHQITGMLHDDPACWNEVDA
                     WIEAARVAHVMFHNRLGLMGHYYGGMLDIYSDMTQQCAVFGGHAEIIEVDELAALRRQ
                     VAAREIQTRVQEFHEAFEIQPDCLPAELERAARTSVALDRLVQIHDLGSMAYYYMGTG
                     NEENEDAISSIILGNSLLTARGIPVAGEFEVKNAQAMKIMDAFGVGGSFTEYYAMDFN
                     DDVILMGHDGPGHIAIAEGMTKVRPLRVYHGKVGRGLSVEMSVKHGPVTLLAVVQTVD
                     GRLKLLVAEGESVLGPILEIGNTNSRYRFPIGARRFMNEWNVHGPAHHCAVGVGHIAH
                     KLEKLAALLQIECARVC"
     gene            complement(3374..3568)
                     /locus_tag="VIL39_09990"
     CDS             complement(3374..3568)
                     /locus_tag="VIL39_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481799.1"
                     /translation="MLAWWLCQHTTARRRWVSERLGMGDESRVTQAIGRLKRKGQPEL
                     ERLKRRLEQVYENRNGAVEV"
BASE COUNT          750 a         1133 c         1109 g          760 t
ORIGIN      
        1 cccccggtgc acttcgacac gaaacctcgg taaccgccca gattgtcgta tttgacgaca
       61 ccgaggatgg tcacgtcgtt gaggagattg tcaatgccgt tggcattggg cacctccatg
      121 tactgcgtgc cggtgacgcg caaggtggcg tggccgttca atgaattggg ggcgtagatt
      181 ggcgagctgg tcacggtggg ctgagtggca ttgttgccca gcccggattg gtcggcccac
      241 gcggtgactt gcgcgctagc gttagtggtt acgccgacat cggcgttcaa ccagagttgg
      301 agaccggcgg tgaccggcgg agtttgcgcc tgcaggccgg tggttgtcca aagagccagg
      361 gcgcagatca ggcggaggag gaggttggtt gctgtttttt tcatagacta acttgggttg
      421 ggggattgga tttgattcat tacaaacagg attggttgcc tatggatggc catcatgccg
      481 tccgccttga agagttgctc ccagcgaagt tgcacacctc gagaatccct gcaaggcatc
      541 tgcgcttgat gtgtcatagt gaggtcactt tacgtgaggc actcgctcag atgcaaggga
      601 cgttctggcc attcttgtgg agattttggc caactcattt ttccccttgc gcttgccgtc
      661 cgctggatgg aagattggag gtgtgccgga acacgcgcca caaacctctt accgttattt
      721 tccagtagca aagcgcgacc ggaattgggg tctcttcatc accaccgtgg gcgaatctca
      781 ccttggcccc ggcacagctt acccgccggc gggccatccg aagggctacg ccttcaatac
      841 tcccaagggg cgccttttgg atgaatacca ggtcatttat atctcttcgg gcagtggctg
      901 gttcaggtct gcggcctccg ggcgggtaag aataaacgcc gggagtgtgc tgcttctgtt
      961 tcccggcgtg tggcacagtt ttggcccggc ggccgccacc ggctggaatg agcactgggt
     1021 gggattcaat ggagagatgg cgcggcgttt ggtgcgccac ggctttttca caccggagcg
     1081 gccagtcctg cgggcgggcg aggagaacaa actgctgggc ctgttcagca gcctcatgga
     1141 agcgacgcgc agcaatcatc cggcgctgca acagattatg gccggaacca cggagtgtgt
     1201 gctggcgctg ctgtattcca cccagcaatc caccctggca ggtgatgacc ccggcttacg
     1261 agtcattcac caggcggtcg ttcgcatacg ggaagccggg gagaccacgc tcaacatgcc
     1321 tgctctggcg agcgaactca aagtcggcta ccgttggttc cggcgggctt ttgcgcacca
     1381 caccgggctg agcccgcatc agtatttcct ggacattcgg ctgacgcgcg ctcgggatct
     1441 gctcgctcag acttcgctct cgatcaagga catcgccttt cgcctcggct ttgaagaacc
     1501 acagtacttt tgtaagctct tccataagaa ggtcggtcta acacccgggg tttggcgggc
     1561 acaggcgcag acgggcaatc tcacccgaac cgggtcgaaa gccgcaagtc ccacggcgtc
     1621 aaaccgagcg ccgtcagctt gaaccggcaa aggcggagac ttcttttggc cacaaactcc
     1681 aatcaaaagg ccaaagtgtc cattgttggc tggcggcatc cggcgtacgg ttgggagaat
     1741 gcagacaacg attcaagacg ccgccctcgc gcacggggag cgcaaagact cctgtacccc
     1801 tcccatttcc gcaccggctc tcagtgggaa aggtgggcaa gctggtccga taatccatga
     1861 agcagcgccg ctcggccaat cgcttgagcc gttctgcgcg ccccgctcca gaccgtccct
     1921 gcgcattggc ttgttcggaa ttggcctgga ggcctattgg cctcagttca aggggttgaa
     1981 ggagcgactc gaaggctatc tcggcatcgt gcaggagcgc ctgcaacgcg acgaggtaac
     2041 cgtcgtcaac ctcgggctca ttgacagccc ccccaaggca ctggaggccg gacaccactt
     2101 tcggcagcag gacgtggacc tgatcttcct ctatgtcacc acctacgcgc tttcttccac
     2161 tgtgctgccc gtggtccaac gcgccaaagt gccggtcatc gtgctcaact tgcaaccggc
     2221 cgcggcgatt gactatgcca gctttaaccg aatgggcgat cgcaccgcca tgactggcga
     2281 atggctggcc cactgcagcg cctgcccggt ccccgagatt gccaacgtgt tcaaccgcgc
     2341 ccgggtcaac tttcatcaga tcacgggcat gttgcacgat gatcccgcct gctggaacga
     2401 ggtggacgcc tggatcgaag cggcccgcgt ggcccacgtc atgttccaca accggctggg
     2461 cctgatgggg cattattacg gcgggatgct ggacatctac tcggatatga cccagcaatg
     2521 cgccgtcttt ggcgggcatg ccgagattat tgaagtggac gaactggccg cgctgcgccg
     2581 ccaggtcgcc gcgcgtgaaa tccagacgcg cgtccaggag tttcacgaag cgtttgagat
     2641 tcagcccgat tgcctgcccg ccgaacttga gcgggcggcc cgcacgtccg tcgccctcga
     2701 ccggctcgtc cagattcatg accttggctc gatggcttac tactacatgg gcacgggcaa
     2761 tgaagagaac gaagacgcca tcagctccat tatcctgggc aactcgctgc tcaccgcgcg
     2821 cggcataccg gtcgccggcg aattcgaggt caagaatgcc caggccatga aaatcatgga
     2881 cgccttcggc gtcggcggct cgtttaccga gtactatgcc atggacttca acgatgacgt
     2941 gatcttgatg ggccatgacg ggcccggcca catcgccatt gccgaaggca tgaccaaggt
     3001 gcgtccactg cgcgtctatc acgggaaggt gggccgggga ctctcggtgg agatgtcggt
     3061 caagcatggg ccggtgactc tcctcgccgt ggtccaaacg gtggacgggc gccttaagtt
     3121 actggtggcg gagggcgaat cggtcctcgg acctattctc gaaattggca acaccaatag
     3181 ccggtatcgg ttccccatcg gagcgcgccg attcatgaac gaatggaatg ttcacggacc
     3241 cgctcaccat tgcgcggtcg gcgttggtca tatcgcgcac aaactggaga aactagcggc
     3301 gctgctccag attgagtgcg cgcgagtctg ctaagatgcc gggccgtgaa taaaagtccc
     3361 ggaattatca catttatacc tccaccgctc cattacggtt ttcatagacc tgctccagtc
     3421 gcctcttcag ccgctccaat tccggctgcc ctttccgctt cagccgtcca atggcctgcg
     3481 tcactcggga ttcgtctccc atccccaacc gctcgctcac ccagcgccgc cgcgccgtgg
     3541 tgtgctggca caaccaccac gccaacacct ccttttccca agcagaaagc agaaagtgcg
     3601 ctcgcccaag gccaccccaa gcgacatcag agccacccca aagccacaat tgggcaaaag
     3661 ctgaaatgct gaaagctgaa agctgaaatc agaaagaggg ctgcacaaag ccacccaatg
     3721 cgacatcaga gccaccccaa agccatttgg ta
//
LOCUS       DATCGS010000489         8292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_734863, whole genome shotgun sequence.
ACCESSION   DATCGS010000489 DATCGS010000000
VERSION     DATCGS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734863"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VIL39_09995"
     CDS             <1..1316
                     /locus_tag="VIL39_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5481800.1"
                     /translation="LGMVGVWAGFPRADFYVAYVLCGLLGLVAIETLIQLVLEMYRPR
                     VKGKVERPLYESRLVGLLGQPEGLVTTAAQALDYQFGFKVSETWFYRFFEKALGWLLL
                     LQLGALVLSTCVVFIDAGEQGLLERFGKRVESRSPLGPGAHLKWPSPIDKVYRFRTEQ
                     IQTFMVGFAEGSGKERERYVLWSQGHHGETNFLVANRAQLSQETTNEVSGKRTPPVSL
                     LTVSVPVQFQITNLLDWAYKNEDAPALLEDLGTREVVRYLVGVDMNDIMSQGRQEASQ
                     ALGDRIQATANEHALGIQIVSVGLGDLHPPVKVAPEYEKVISAIQTKQAKILVARTED
                     IRTNALAEAQATNLLNKANAECLAREVGALAQAALFTNQIPAFKAAPSVYVERAYLQT
                     FVRSTANARKYVLLTTNTHDVLIFDLQESIANSLLNLSVPAPKTK"
     gene            1322..2233
                     /locus_tag="VIL39_10000"
     CDS             1322..2233
                     /locus_tag="VIL39_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY5481801.1"
                     /translation="MKRNPLTLIIGLLLIIIFGLLLFTFQVRTTEVAVVTTFGKPTRP
                     ITEPGIYAKAPWPIQKVWTFDQRVQNFEDRLTEGLTQDSFNLLTSVYVGWKVSNPTNF
                     FPRFAGSANPIAAAETLLDQWLGNAKTAVVGKHPLADFISTTDNGTNFVAIENEILAR
                     IQSQLQTNNLGLEVQFLGIKRLQLPESVSQSVFDRMTSERKRLAERFQSEGERDAQRI
                     RSDAERRAAELLANAESQATRIKGIGEAEAAKSLAVFQQNPELASFIFRLNALESSLK
                     ERSTLIFDQQTPPFDLFRGASTNLLHK"
     gene            2241..3338
                     /locus_tag="VIL39_10005"
     CDS             2241..3338
                     /locus_tag="VIL39_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEY5481802.1"
                     /translation="MNDQPHSHDGLPEPALPPETPMDAGSQALAEALRSSFAIIKVVM
                     VVLVLLFLASGFFTVGPQERAILLRFGRPVGEGEKALLLPGLHWSLPYPIDESIKVSI
                     SGLQQVSSTVGWYAVTPEQMLAGIEPPSGGMLNPIVDGYALTADVNIIHTRATLTYRI
                     NDPIRYVFSFVNASNAVQNALDNALLYAASRFEVDDILTRDITGFQEEVRRRTTQLVE
                     HQDLAISIEQCVVGSVPPRQLKDAFANVLKAEVTRSKVLNEARSSENQVTNKASADAG
                     SLINLAESDRARRVSDMAAQAQRFLEILPQYQDHPALFAQQRLTETLGRVLTNAQDKI
                     FLAESPDGNRKELRLLLNREVPKPKTEETTP"
     gene            3375..5393
                     /locus_tag="VIL39_10010"
     CDS             3375..5393
                     /locus_tag="VIL39_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY5481803.1"
                     /translation="MQVTSLLSRHQHEHDETCVTCGHDHEHAPVRLKQTLFGVVFVLN
                     AFIVDWLFDQGHTVASASGLIGAVILGYPIVWTAIKDIRTGVLSINELVAIAVLAAFA
                     TGEYKTAGVVAFFMLIGEIIETRTAEGARASIEHLIKLTPTKARRITPQGEEEVAAQA
                     LAIGDVIRVRPGDNVAADGVILSGQGSFNQATITGESLPVDKKPGDEVFAGTQNLTGV
                     LEIKVSRAGTDTTLGRVRELILAAEKTKLPIMKIVDQYMGFYTPLVLVIAALVWAFTG
                     KEHGLQNVISVIIFSCPCAFILATPTAMVAALSAAARLGILIKNVADIELAAKINAFV
                     FDKTGTLTTGELAVSRLAPLNGVTPSELLRVAASAEQYSNHPTAKALTQLASEASVPL
                     AEPKDFAEIAGRGVKAEIGGVKVLVGRAQWLRDNGVQEDFLKSVDLNETEGWSLIFVA
                     REGRCIGWVGLQDQTRAEARESLLELKASGVRRIAMVSGDRQPVATRVAREIGCEEAK
                     GECLPQNKVEFVRAIKAKGYTVAVVGDGVNDAPALAAGDIGIAMGAAGSEVAIHSATI
                     ALMNNDLRRLPFLVKLSHSTRTVINQNFLFGVAFIIGGMTLSALSYVPPVWAAAMHTG
                     GSLIVVFNSARLVRKGEELEHYQPEPAAPKPERPAAPSPKAELAPKTA"
     gene            5403..6593
                     /locus_tag="VIL39_10015"
     CDS             5403..6593
                     /locus_tag="VIL39_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481804.1"
                     /translation="MTTTDTPARSDGEVVVSVRGLTKVFKDFWGRPKARAVDNADFEV
                     RRGEVFGLLGPNGSGKSTTVKLILGLLHPTKGHIEVFGHSPRHVATKSRIGYLPEESY
                     LYRYLNSRETLDFFGNLFRLPRNERNDRAEQLLEMVGLSQTRTRAVGEFSKGMQRRIG
                     LAQALINDPDLVILDEPTSGLDPIGCREVKDLILALARRGKTVILSSHLLGDVEDVCD
                     RVVIYYGGKVQARGTLKELLATPDAIRITTPALPRETMERVLALIRKDVAEDKVKIDT
                     PTQNLESYFLDVVLKARQASAETSGAMSGAHVAAYLRGQGEATLAPDRILERLTAPQA
                     AAPTPAPALQPVETLSQQKLEAMTKPAELVPPAPTAAPAAAKPLSAEELRRADEKLAS
                     LVRK"
     gene            6634..8013
                     /locus_tag="VIL39_10020"
     CDS             6634..8013
                     /locus_tag="VIL39_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5481805.1"
                     /translation="MQPLLAIAWLTWKAAFRFRLFLVVAVLLLGSVVGLPLLIKDDGT
                     ARGFTQILLTYTLGTISALLGLSTLWLACGTLARDLEDCTIQVVAVKPIARWQIWLGK
                     WLGIMSLNAALLTLSGASVYGLLQWRATRLPADQQKILRSEVLVSRGSVKPANLDKEI
                     QAETDQLLRERLKKNPDLKADIAEARKQILEQVRAEYQVVPSGYVHEWEVDLGPAKSL
                     ARGQPIYLRVKFNAADRSTSGTFDGRWRIGVPRKTPLWHSDVMSLAPDTFHEFEVPAN
                     YDDKGVLTVAFANANNTALLFPLEDGLEVLYSQGGFGLNFMRGVGIIFCWMALLATLG
                     LTSASLLSFPVAAFLSLGVLTLALSSGTISNAVSEGTLMGYNSETSQAGHSPLDVVAI
                     PVFRAVLGVINLAKNFSPIDSLSTGRSVSWGELGQAFVQIVLLLGGSIGLTGIIIFNR
                     RELATAQAQ"
     gene            8010..>8292
                     /locus_tag="VIL39_10025"
     CDS             8010..>8292
                     /locus_tag="VIL39_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481806.1"
                     /translation="MSVILRIIPSLSLIGALLVGAGLVQQSLNQDRDQLGLTRVQPLE
                     NAPPVLAFTTVALGGFRGLISNALWMRASDLQDEDKFFEMAQLADWITKL"
BASE COUNT         1568 a         2555 c         2625 g         1544 t
ORIGIN      
        1 cgctgggcat ggtgggggtc tgggccggct ttcccagggc ggacttctac gttgcatacg
       61 tgctgtgcgg cctgttgggt ttggtggcga tcgagaccct gatccaactg gtgctggaaa
      121 tgtaccggcc gcgggtcaag ggcaaggtcg agcggccgct gtatgagagc cggctcgtcg
      181 gcctgctggg gcagccggaa ggactggtca ccacggcggc gcaggcgttg gattatcagt
      241 tcggctttaa ggtttccgaa acctggttct accgcttctt cgagaaggcg ctcgggtggc
      301 tcctgctgct gcaactgggc gcgctggttt tgtccacctg cgtggtgttc attgatgcgg
      361 gtgagcaggg gttgctcgaa cgtttcggca agcgcgtgga gagccgttca ccgctcggcc
      421 cgggcgccca tctgaaatgg ccgtcgccca ttgacaaggt ctatcgcttc cgcaccgagc
      481 agattcagac ctttatggtg ggcttcgccg aggggtccgg caaggagcgg gagcggtacg
      541 tgctgtggag tcagggccac cacggggaaa ccaattttct ggtggcgaac cgggcgcagc
      601 tctcacagga aaccaccaac gaagtctccg gcaaacgcac cccgcccgtg agcctgctga
      661 cggtgagcgt gccggtgcaa tttcagatca ccaacctgct ggactgggcc tataaaaacg
      721 aggacgcccc ggcgcttctc gaggacctgg gcacgcgcga ggtggtgcgc tacctcgtgg
      781 gggtggatat gaatgacatc atgtcccaag gccggcaaga ggcatcgcag gcgctgggcg
      841 accgcatcca ggcgacggcc aacgagcatg cgctgggcat tcagatcgtc tcggttggcc
      901 tgggggacct gcacccgccg gtaaaggtcg cgccggaata cgagaaggtc atctcggcca
      961 tccaaaccaa gcaggcgaag atcctcgtcg cccgcacgga ggacatccgc accaatgcgc
     1021 tggccgaagc gcaggccacc aaccttttga acaaggccaa cgccgaatgc ctggcgcgcg
     1081 aggtgggcgc gctcgcccag gccgcgctct ttaccaacca gattcccgcc tttaaggcgg
     1141 caccctccgt ctatgtcgag cgggcctacc tgcaaacctt tgtccgatcc accgccaacg
     1201 cgcgcaaata cgttttgctc acgactaaca cgcacgacgt cctcattttc gacttgcagg
     1261 agtccattgc caatagcctg ctcaacctga gcgtgcccgc acccaaaacc aaatagttgt
     1321 catgaaacgc aatcccttga ctctcattat cggcctgtta ctgatcatta tcttcggcct
     1381 gctcctgttc acgtttcagg tgcgcaccac cgaagttgct gtcgtgacca cctttggcaa
     1441 accaacgcgc cccatcaccg agcctggtat ctatgcgaaa gcgccctggc ccatacagaa
     1501 ggtctggacc ttcgatcagc gggtgcagaa tttcgaggac cgccttaccg aaggcctgac
     1561 gcaagacagc ttcaacctgc tgacctcggt ctatgtgggc tggaaggtct cgaacccgac
     1621 caatttcttc ccccgcttcg ccggcagcgc caatccgatt gcggcggcgg aaacgctcct
     1681 cgaccaatgg ctgggcaacg ccaagacggc cgtggtgggc aagcatccgt tggccgattt
     1741 catctccacc accgacaatg gcaccaactt tgtcgccatc gagaacgaga tcctggcgcg
     1801 tatccagtcg caactccaga ccaacaacct gggtctggag gtgcaattcc tcggcatcaa
     1861 gcgcctgcaa ctgccggaaa gcgtcagcca gtcggtgttc gaccgcatga cctccgagcg
     1921 gaaacggctg gccgagcggt tccaatccga aggcgaaagg gacgcgcagc gaatccgatc
     1981 ggatgccgag cgccgggcgg cggagctgct ggccaacgcc gaatcgcagg cgacccggat
     2041 taagggcatc ggcgaggcgg aagccgccaa gtcgctggcc gtcttccagc agaatcccga
     2101 gttggccagc ttcatctttc gcctgaacgc gctggaaagc tccctgaagg agcgctcgac
     2161 gctcatcttc gaccagcaga cgccgccgtt tgacctgttc cggggcgcct cgaccaacct
     2221 gctgcataag tgagccggca atgaacgacc aaccacattc ccacgatggg ctgcctgaac
     2281 ctgccctgcc gccggagacg ccgatggatg ccggctcgca ggcgctggcc gaagccctgc
     2341 gcagcagctt tgccatcatc aaagtcgtga tggtggtgct cgtgctgctg ttcctggcgt
     2401 ccggcttttt cacggtcggc ccccaggagc gggcgattct gttgcggttc ggccgaccgg
     2461 tgggcgaagg cgaaaaggcc ttgctcctgc cggggcttca ttggtcactc ccttatccca
     2521 ttgacgagag catcaaggtc tcgatttccg ggctgcagca ggtgagttcg accgtgggct
     2581 ggtacgccgt gacgcccgag cagatgctgg ccggcattga acccccgtcg ggcggcatgc
     2641 tcaatcccat cgtggatggc tacgccctga ccgccgatgt taacatcatt catacgcgcg
     2701 ccaccttgac ctatcgcatc aatgacccga ttcgatacgt tttcagcttc gtcaacgcct
     2761 ccaacgccgt gcagaacgcc ctggataacg ccctgctcta cgctgcctcg cgcttcgagg
     2821 tggatgatat cctcacccgg gacatcaccg gcttccagga ggaggtacgc cggcgcacga
     2881 cgcagttggt ggagcatcag gatctggcga tcagcattga gcaatgcgtg gtcgggagcg
     2941 ttccgccgcg gcagttgaag gacgccttcg ccaacgtgct caaggccgaa gtgacgcgca
     3001 gcaaggtgct caacgaggcg cgcagctccg agaatcaagt caccaataag gccagcgccg
     3061 acgccgggtc gctcatcaat ctggcggagt ccgaccgcgc ccgccgcgtg agcgacatgg
     3121 ccgcccaggc gcagcggttc ctggagattc tgccgcaata ccaggatcac cccgccctgt
     3181 tcgcccagca gcgcctgacc gaaacgctgg gccgtgtctt aaccaacgcg caggataaga
     3241 tatttctggc ggagagccct gatggcaacc gcaaggaatt gcgcctattg ttgaatcgtg
     3301 aagtcccgaa gccgaagacg gaagagacca caccgtgatg ggcgctgaaa gccactaatt
     3361 gattttttaa ggccatgcaa gtcacctctc ttttaagccg acaccagcac gagcacgatg
     3421 aaacgtgcgt gacgtgcggt cacgaccacg agcacgcgcc tgtgcgattg aagcagacgc
     3481 tgttcggggt cgtgttcgtc cttaatgcgt tcatcgtgga ctggctattc gatcagggcc
     3541 acacggtcgc cagcgccagc ggtttgatcg gtgccgtcat cctcggctat ccgattgtct
     3601 ggacggccat caaggatatc cgcaccggag ttctgagcat caacgaactg gtcgccatcg
     3661 ccgtgctcgc cgcgtttgcc accggcgaat acaagacggc cggcgtggtc gcctttttca
     3721 tgctgatcgg cgaaatcatt gaaacccgca ccgccgaagg cgcgcgcgcc tccatcgagc
     3781 acctcatcaa actcacgccc accaaagccc gccgcatcac cccgcaaggg gaggaggaag
     3841 tcgctgcgca agccctggcg atcggtgacg tgatccgcgt ccgtcccggc gacaacgtgg
     3901 cagcggacgg cgtcatcctc agcggccagg gctcgtttaa ccaggctacg atcacgggtg
     3961 aatcgttgcc tgtggataag aagccgggcg acgaggtctt cgccggcacc caaaacctga
     4021 ccggcgtctt ggagatcaag gtaagccggg cgggcaccga caccacactg ggccgcgtgc
     4081 gcgagctgat cctggcggcg gagaagacca aactcccgat catgaagatc gtggaccagt
     4141 atatgggttt ctacaccccg ctggtgctgg tcatcgccgc gctggtgtgg gccttcacgg
     4201 gtaaggaaca cggcctgcag aacgtcatct cggtgatcat cttcagttgc ccttgcgcct
     4261 ttattctcgc tacgccgacg gcgatggtgg ccgcgctgtc ggccgccgcg cgcctgggta
     4321 ttctcatcaa gaacgtggcc gacatcgaat tggcggcgaa gatcaatgcc tttgtttttg
     4381 acaagacggg cacgcttacg accggggaac tggcggtgag ccgactggcc cccctcaacg
     4441 gcgttacccc ctccgagttg ctgcgcgtcg cggcgtccgc ggagcaatac agcaatcacc
     4501 cgacggccaa ggcgctgacg caattggcgt cggaggccag cgtgccattg gcggaaccga
     4561 aggattttgc cgagatcgcg ggccgcggcg tgaaggccga gatcggcggc gtcaaagtgc
     4621 tggtgggccg ggcgcagtgg ctgcgcgaca acggcgtgca ggaagacttc ctgaagtcgg
     4681 tagatctcaa cgaaaccgaa ggctggagcc tgatattcgt cgcgcgcgag ggccggtgca
     4741 tcggctgggt cggtttgcag gaccaaaccc gcgctgaagc gcgggagtct ctgctggagt
     4801 tgaaggccag cggcgtgcgg cgcattgcga tggtttccgg cgaccgtcag ccagtggcga
     4861 cccgggtggc gcgcgagatc ggttgtgagg aagccaaggg cgagtgcctg ccccagaaca
     4921 aggtcgagtt tgtgcgcgcc atcaaggcca agggctacac ggtggccgtg gtgggcgacg
     4981 gcgtgaacga cgcgcccgcg ctggccgccg gcgacatcgg catcgccatg ggcgcggccg
     5041 gcagcgaggt ggcgattcac agcgccacca tcgcgctgat gaacaacgac ctccggcggt
     5101 tgccgttcct cgtgaagctg tcgcatagca cccgcacggt catcaatcag aacttcctgt
     5161 ttggcgtggc ctttatcatc gggggcatga cactgtcggc gctgagctat gtgccgcccg
     5221 tatgggccgc ggcgatgcac accgggggct cgctgatcgt agtcttcaat agcgcccgcc
     5281 tcgtgcgcaa aggcgaagaa ctcgagcact atcagccgga gccagcggcg cccaagccgg
     5341 aacggccggc cgcgccaagc ccgaaggccg agttggcgcc caaaacggcg tgagctaaac
     5401 acatgaccac aactgacact cccgcgcgtt ccgacggcga agtggtcgtt tccgttcgcg
     5461 gcttgaccaa ggtcttcaag gatttctggg gccggcccaa ggcgcgcgcg gtggataacg
     5521 ctgatttcga ggtgcggcgc ggggaagtct tcggtctgct cgggccgaat ggttccggta
     5581 agtccactac cgtgaagctg attctgggct tgttgcaccc gacaaaaggg cacatcgagg
     5641 tcttcggtca ttcaccccgc cacgtcgcaa ccaaatcgcg catcggctac ctgcccgagg
     5701 agtcttacct ttaccggtac ctcaattccc gtgagacgct ggatttcttc ggcaacctct
     5761 tccgcctgcc ccggaatgag cgtaatgacc gggcggagca gttgcttgaa atggttggct
     5821 tgagccagac acggacgcgg gcagtgggcg agttctcgaa aggcatgcag cgccgcatcg
     5881 gcctggcgca ggcgctgatc aacgaccccg acctggtgat tctcgacgag ccgacttccg
     5941 gcctcgatcc catcggctgc cgcgaggtca aggatttgat tctggcgctg gcgcggcggg
     6001 gcaagacggt gatcttgagc agccatttgc tcggggacgt ggaagacgta tgcgaccggg
     6061 tggtgattta ttacggcggt aaggtccagg cgagaggtac gttgaaggaa ttgttagcga
     6121 cacccgatgc gattcgcatc accactccgg cgctcccccg cgagaccatg gagcgcgtgc
     6181 tggccctcat tcgcaaggac gtggcggagg ataaggtcaa gatcgacacg ccaacccaga
     6241 acctggagag ctactttctc gacgtggtgc tgaaggcgcg gcaggcctca gcggaaacct
     6301 ccggcgctat gtccggcgcc catgtcgccg catacctgcg tggccagggg gaggccacac
     6361 ttgcgcctga caggattctg gagcggctca ccgcaccgca agctgctgcg cccactccgg
     6421 cccctgcgtt gcagccggtc gagactctga gccagcaaaa gctggaagcc atgacgaaac
     6481 ccgcggagct ggtccccccg gcgcccacgg cagcgcctgc ggcggcaaaa ccgttgagcg
     6541 cagaagagct gcgcagggcc gacgagaagc tggcctcctt ggtgaggaaa tagaacgggt
     6601 aacttccagc cgccctttaa gcgcgtttga ttcatgcaac ccttgcttgc catcgcctgg
     6661 ttgacctgga aggccgcgtt tcggttccgg ttgttcctgg tggtggcggt cttgttactg
     6721 gggtccgtgg tgggtctgcc gttgctgatc aaggatgacg ggacggcgcg cggcttcacc
     6781 caaatcctgc tgacctatac gctgggcaca atcagcgccc tgcttgggct ttccacgctc
     6841 tggctggcgt gcggcaccct ggcgcgcgac ctcgaggatt gcaccattca ggtggtcgcc
     6901 gtcaaaccga ttgcccggtg gcaaatctgg ctgggcaaat ggctgggcat catgtcgctg
     6961 aacgccgcgc tgctaacctt gtccggagcc agtgtctatg gcttgctcca gtggcgcgcg
     7021 acgcgattgc ccgcggacca gcagaagatt ctgcgcagtg aagtgctggt gtcgcgcggc
     7081 tcggtgaagc cggcgaacct cgacaaggaa atccaggcgg agacggatca gttgctgcga
     7141 gaacgcctga agaagaatcc agaccttaag gccgatattg ccgaggcccg aaagcaaatc
     7201 ctggaacagg tcagggccga ataccaggtc gtcccgtcgg gatacgtgca tgagtgggaa
     7261 gttgatttag gaccggcgaa gagcctggca cgcggtcagc cgatctatct gcgcgtgaaa
     7321 ttcaatgccg cggacaggag cacttccggc acgtttgacg ggcggtggcg gataggcgtg
     7381 ccgcgcaaaa cccctttgtg gcacagcgac gtgatgagcc tggcgccgga cacctttcat
     7441 gagtttgaag tcccggcgaa ttacgacgac aagggcgtgc tgaccgtggc cttcgccaac
     7501 gcaaacaaca ccgcgttgct gttcccactg gaggacgggt tggaggtgct ttactcccag
     7561 ggcggctttg ggctcaattt catgcgcggg gtggggatta tcttctgctg gatggcgctg
     7621 ctggccaccc tcgggctgac ctcggccagt ctcctttcct ttcccgtggc agcgttcctg
     7681 tcgttgggcg tcctgactct ggcgctctcg agcggaacga tctcaaatgc ggtgtccgag
     7741 gggacgttga tgggttacaa ttcggagact agccaagcag ggcactcgcc gctcgatgtg
     7801 gttgccattc cggtctttcg ggcggtgctt ggggtgatca acctggccaa gaacttttcg
     7861 cccattgatt cgctcagcac cggccggagc gtctcctggg gagagctcgg ccaggcgttc
     7921 gtgcagatcg tgctgctgct cggcggcagc atcggactga ccggcatcat cattttcaac
     7981 cgccgcgaat tggcgaccgc ccaggcccaa tgagcgtcat cctgagaatc atcccatcgc
     8041 tttcgctcat cggcgcgctg ctggtggggg ccggattggt gcaacagtcg ctcaatcagg
     8101 accgggacca gctcggcttg acacgggtgc aaccgctgga gaacgcgccg cccgtgctcg
     8161 ccttcaccac ggttgcgctg ggcggttttc gcgggctgat ttccaacgcg ctgtggatgc
     8221 gcgccagcga cttgcaggac gaggacaagt tcttcgagat ggcccaactg gcggattgga
     8281 tcacgaagct cg
//
LOCUS       DATCGS010000490         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_734937, whole genome shotgun sequence.
ACCESSION   DATCGS010000490 DATCGS010000000
VERSION     DATCGS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734937"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..785
                     /locus_tag="VIL39_10030"
     CDS             348..785
                     /locus_tag="VIL39_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481807.1"
                     /translation="MRLAGLPDESGVPVVVSGCTQYSPTPSHFLVVTKEEKNDHLLLE
                     ILTTGATLRYHTIADRPEAHLLFQSLGERRVKDSAVNLALFVQELAKFAPGVLLNHGA
                     YYMCENSNPAFTYPSPTAFYREITWLLWMVASGRTQPSRDSAV"
     gene            complement(857..2791)
                     /locus_tag="VIL39_10035"
     CDS             complement(857..2791)
                     /locus_tag="VIL39_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEY5481808.1"
                     /translation="MRIVILALAGFVLFFCPVARAWTSAGHGLSETNSWSLSSPDGKC
                     EICVSLDGNRLSYFALYRGKVAIQRSPLGLQRDDQDFQSNLAFGHAGEIYSRREQYEL
                     YAGIQPRVDHLLNHRHLVFRNTNRVALEIELAAGTEGVAFRYHFGETNGATCVVKSES
                     TGFKLPLNATGWLQPYHAAGPYTPAYEDFYFHVSPAQSPPESRAKPRGWAFPALFRIP
                     DPGVWALLTESGTDDSYCGCHLDGDSSGGLYHIAFPLADEATKGFVTKVGPEPRNSLP
                     WTMPWRVIVLGESAGEIALQTLVTDLAPPSAIPDTAWVHPGRASWAWWSYPEEPPTAK
                     RFDEFTDLAAKMGWEYTLFDAGWWKPGLKPIASYALAKGVAPLAWLHAADFYGPKSRT
                     KKLDEMAAAGIRGVKVDFWCSDRQEAIAAQQALMKEAAARKMVVNLHGCTIPRGWQRT
                     WPNFLTCEAVLGSESYFYEPRYTQKAAELDTVLPFTRNAIGPMDLTPVACSPKKYARL
                     TTAAHQLATALIFTSGIVHYADQPQFFESLPSEALQVFRDAPARWDETRCLVGEPGRA
                     VVFARRSRQAWFIAGISGISEPLPLTLDLSVFRRHHHWTLIAEGADSNMQVSVAPLEG
                     AGEWRHEIPPRGGFVLRLDE"
     gene            complement(3021..4316)
                     /locus_tag="VIL39_10040"
     CDS             complement(3021..4316)
                     /locus_tag="VIL39_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY5481809.1"
                     /translation="MRARWIHWRSVLPIGWLALLMAGCGPKPAAPARRITSIHVTGVY
                     SEAARALAGDFERETGIHVNVVEANLFSLREKELTDVLTRGGHYDVMQVAYQWEGEIL
                     PHLRPLDDVVQQIAPDLADFIPTVRTNCGQWGGRTYGLPMACDVITLLYRTDLFEARS
                     AEFERLTGRPLQPPRTWPEYLEIARFLNSESLYGNILMGGEQTYTVWSGILHGMGGRQ
                     VDEHWRPVLNSEAGVRSLALFAEMYQYAPPHSEGRAIHDADTLFLQGRGAMYLAWPSL
                     IWSQLADPSRCKITGKIGAAVIPGGQPQLSAWSLGVNPACQDPEAAYLWIKFFVNQSN
                     SRRLLLLYGKGSPRISTYSDPQCREKLFYLKTLLEGLAGNQPRFRIPPSQELSDYLDN
                     ELIKAIRGQVTPRAALDRTAARWREILTQSGHLSESASP"
     gene            complement(4316..4984)
                     /locus_tag="VIL39_10045"
     CDS             complement(4316..4984)
                     /locus_tag="VIL39_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5481810.1"
                     /translation="MRTGSSGAKTVVWLIEDHADSRRVLARVLNQASTMQCPCAFASC
                     EDALAALQTNPHPDVILLDIGLPGMSGIEGIQRLKALSPATHVIMLTVFDDQEKVFNA
                     ICAGASGYLLKTADEDAVADAIQEVLEGGAPITPRIARLVLNMFASQSAPPAQNYGLS
                     DREREVLELMVQGFIKKEVADRLHLSYHTVDNHFRSIYTKLLVHTRGGAVAKAVSEHL
                     VAPG"
BASE COUNT         1049 a         1554 c         1553 g          977 t
ORIGIN      
        1 cctctgcgct ctgaaaacag aggccgcgag ctgcgattga taccatgaaa aacccgcggt
       61 gactgccgga tttgcatggt gcatgatcat ggggtgattg atcgcaagac agaccttggg
      121 gattagtggt cattgatgaa atccagcgcc gccccgatct ctttccactg ctacgcgcgc
      181 tggctgaccg cgcgccgctg cctgcaacag ccgccgacct gaggaggttc cgcattggcg
      241 caagcctcca ttactccatc actccattac tctcgggcac atcctgacac caccaccaaa
      301 gcgggcggct atgccaatcg gaacgccgga ttcatccggc agccccagtg cggctcgccg
      361 gcctgccgga tgaatccggc gttccggtgg tggtgtcagg atgcacccaa tactctccca
      421 ccccctcaca tttcctggtg gtgacaaaag aagagaagaa tgaccatcta ttgctggaaa
      481 tcctcacgac cggtgccacg cttcgctacc acaccattgc ggatcggccc gaagcgcacc
      541 tgctcttcca aagcctgggg gaacggcggg tcaaagattc cgccgtgaat cttgcccttt
      601 ttgttcagga attggcgaaa ttcgcgcccg gggtgctcct caatcatggc gcctactata
      661 tgtgcgagaa ctctaacccg gcgtttacct atccaagccc gacggcgttt taccgggaga
      721 tcacctggct cttgtggatg gtcgcctctg gccgcacgca gccatctcgc gactccgcgg
      781 tgtaacaacg gcaatcgctt ttcgcactcc tcacctcggg cgtttgcaaa tacctgctca
      841 tggaatgaat ccgtgattat tcgtccagac gaagcacgaa gccgccgcgc ggtggaatct
      901 catgacgcca ttcacccgcg ccttccagcg gcgcaacgct cacctgcatg ttcgaatcgg
      961 caccctctgc gataagagtc cagtgatgat ggcgtctgaa tactgacaaa tccagagtta
     1021 gcggcagagg ttcgctgatt ccgctaatgc cggcgatgaa ccaggcttgc ctggaacgcc
     1081 gagcgaagac caccgcgcgc ccaggctctc ccaccaggca acgggtttcg tcccagcgcg
     1141 ccggggcgtc tcggaacact tgaagcgcct cggatggcag cgattcgaag aactgcggtt
     1201 gatcggcata atggacgatg ccggatgtga aaatgagcgc cgtcgccaat tgatgtgcgg
     1261 cagtcgtcag gcgcgcatat ttcttgggcg agcacgcgac cggcgtgaga tccatcgggc
     1321 caatagcgtt gcgggtgaag ggcagaacgg tgtccaactc tgccgccttt tgagtgtaac
     1381 gcggctcata aaaataggat tcgctcccca gaaccgcttc gcaggtcagg aaatttggcc
     1441 aggttcgttg ccagccgcgg gggatggtgc agccgtgcag gttcactacc atcttgcgtg
     1501 cagcggcctc tttcatcaat gcctgctggg cagcgatggc ttcctgacgg tccgaacacc
     1561 agaagtccac ctttactccg cgaatgccgg cggcggccat ttcatccagt ttcttcgttc
     1621 tcgatttcgg cccataaaaa tccgcggcat ggagccaggc cagaggagcg acgcctttgg
     1681 ccaaggcata gctcgcgatg ggttttaatc cgggcttcca ccaaccggcg tcgaacaagg
     1741 tatattccca tcccatcttg gcggccaagt cggtgaactc atcgaaccgc tttgcggtag
     1801 gcggctcttc agggtacgac caccaggccc acgacgcgcg gcccggatgc acccaggccg
     1861 tatccggaat ggccgacggc ggggcaagat cggtgacgag cgtttgcagg gcaatttcac
     1921 ctgccgattc cccgagcaca atcacgcgcc agggcattgt ccagggcaac gagtttctcg
     1981 gctcaggacc caccttggtc acaaaaccct ttgtggcttc atcggccaaa ggaaacgcga
     2041 tatgataaag tccgccggaa gaatccccgt ccagatgaca gccgcagtaa gaatcgtctg
     2101 tgccagattc tgtcagcaaa gcccacacgc ctgggtcggg aattcgaaaa agagcgggaa
     2161 aagcccagcc tcttggcttt gcgcgcgatt ccggaggtga ctgggccggg gagacatgaa
     2221 aatagaaatc ctcgtaggcc ggcgtatatg gacccgccgc gtggtaaggt tgcaaccagc
     2281 cagtcgcatt caacggtagc ttgaaaccag tggattccga ttttacaacg caagttgcgc
     2341 cattggtttc gccgaaatga tagcggaagg ccacgccctc cgtgccggct gccagctcga
     2401 tttccagtgc tacacgattg gtattgcgaa acaccagatg gcggtggttc aacagatgat
     2461 ctacgcgcgg ctggattccg gcgtataatt cgtattgttc gcgacggctg tagatttcgc
     2521 cggcgtgacc aaaggctaaa ttgctctgaa aatcctgatc gtcacgctgt agccccaacg
     2581 gtgacctttg aatcgcgacc ttgccgcggt acaaggcgaa ataactcaag cggttgccgt
     2641 caagtgagac gcagatctcg cacttcccgt caggggaact cagcgaccac gaattggttt
     2701 cgctcaggcc atgtccagcg ctggtccagg ctcttgccac aggacagaag aacaggacaa
     2761 atcccgcaag cgccaaaatt acaattcgca tggcgagagg ggtattatgg cagtggcggt
     2821 caggcaataa aaatgactag acgggagggc agggggattt acgggaatgc cgtcgtgtca
     2881 agtgaagggg cgcggtccgc tgcccttggc tatgccaatc ggaacgccgg attcatccgg
     2941 cagccccaat gcgactcgcc ggcctgctgg atgaatccgg cgttccggtg gtggggtcag
     3001 aatgcgctcg cgtcagggcg tcagggcgag gcggattcgc tcagatgacc gctctgagtc
     3061 aggatttccc gccagcgcgc ggcggttcga tccagcgcgg cgcgcggggt gacctgtccg
     3121 cggatggctt tgatcagttc gttgtcgagg tagtcggaca gctcctggga gggcggaatg
     3181 cgaaagcgag gctggttgcc cgccagacct tccagcaacg tcttgagata aaagagtttc
     3241 tcccggcact gggggtccga ataggtcgag attcgcggcg agccttttcc gtaaaggagc
     3301 agcaaccgcc ggctgttgct ttgattgacg aagaacttga tccagagata agccgcctcg
     3361 ggatcctggc aggccgggtt cacgcccagc gaccacgcgc tgagttgcgg ctgaccgccc
     3421 ggaatcaccg ccgccccgat cttcccggta atcttgcagc gactcgggtc cgccaactgg
     3481 gaccagatca agctgggcca ggccagatac atcgcgcccc gcccttgcag aaagagcgtg
     3541 tcagcgtcgt ggatggcccg gccctcggag tgtggcgggg cgtattgata catctcggca
     3601 aacagcgcga ggcttcgcac gccggcttcg gagttgagca ccggccgcca atgctcgtcc
     3661 acctgccgcc cccccatccc atgcaggatg cccgaccaga ccgtgtaggt ctgttccccg
     3721 cccatgagga tattgccata gagcgactcg gaattcagaa accgggcaat ctccagatac
     3781 tccggccagg tcctcggcgg ttgcagcggg cggccggtca atcgttcgaa ctcagccgag
     3841 cgggcctcga aaaggtcggt ccgatacaac aacgtaatga catcgcacgc catcggcagg
     3901 ccataggtgc gcccgcccca ttggccgcaa ttggttcgca ccgtcgggat gaaatccgcg
     3961 aggtcgggag cgatctgctg gaccacgtca tccagcggac gcagatgcgg cagaatctcg
     4021 ccctcccact ggtaggcaac ctgcatgacg tcgtagtggc cgccacgcgt cagcacatcc
     4081 gtgagttcct tctcccgcaa gctgaacaga ttggcctcga ccacattgac gtgaatgccg
     4141 gtttcccgct caaagtcgcc agccaacgcc cgcgcggctt ccgagtaaac acccgtcacg
     4201 tggatggacg tgatcctccg ggcaggcgct gcgggtttcg gcccgcaacc cgccatgagc
     4261 aaggcaagcc agccgatggg cagcacgctt cgccaatgaa tccagcgggc gcgcatcagc
     4321 cgggggcaac caggtgttcg gagacggctt tggcaaccgc gccgccccgg gtgtgaacga
     4381 ggagcttggt gtagatgctt cggaaatgat tatccacggt gtggtagctg aggtggagcc
     4441 ggtcagcgac ctccttcttg ataaagcctt gaaccatcaa ctccaacacc tcacgttcgc
     4501 ggtccgacaa gccgtaattc tgcgcgggcg gcgcggactg ggaggcgaac atgttcagca
     4561 ccaggcgcgc gatccgcggg gtgatgggcg cgccgccttc aagcacctcc tgaatggcgt
     4621 cggcgacggc atcttcgtcg gcggttttca agaggtaacc cgacgccccg gcgcaaatgg
     4681 cgttgaagac cttctcctga tcgtcaaaca ccgtgagcat gatgacatgg gtcgcgggag
     4741 agagcgcctt gaggcgctgg atgccttcga tgccgctcat gcccggcagc ccgatgtcca
     4801 gcagaatgac atccgggtgc gggttggtct ggagcgcagc cagcgcgtcc tcgcacgagg
     4861 cgaacgcgca gggacattgc atcgtcgagg cctggttcaa cacgcgggcg agcacgcggc
     4921 gggagtcggc gtgatcttca atcagccaga ccactgtctt cgcgcctgag gaaccggttc
     4981 gcatggggct attcttcgct ttttgtcgag ggtgggcaag tctcgggagg actctagcca
     5041 gggcgcaggg aagacagcgt caggcgggtc ccctggccgg gccggctttg aacctcaaag
     5101 gtcgctttca tgcgcgcggc ccgggcgcgc aga
//
LOCUS       DATCGS010000491         5595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_537786, whole genome shotgun sequence.
ACCESSION   DATCGS010000491 DATCGS010000000
VERSION     DATCGS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537786"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            739..1593
                     /locus_tag="VIL39_10050"
     CDS             739..1593
                     /locus_tag="VIL39_10050"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481811.1"
                     /translation="MASTLSPSEEAQLGQTIEMFEVITQSQPHDYQSLEILKEAYSKL
                     NREKDVISTSKRIAQAYVQMGQLSSAILEYETILQRYPDDPDVRTALQQIESKANSFP
                     LETALSADTVLLTRASAEGDKPRKAASKEAPAEIEDGRRLMHKLFVESKVITAGDFAL
                     CWSTQDLTLPPGGVMEPFIQVLSDKGILPVDKSLKILCDKSRFAFLPLNSYDIDLELA
                     RKFPRVTCQRWCVLPFDRMSKSVLVATANPFNQQAAKELAEATPNRLLWYLVPPSDLV
                     ANVRKAFR"
     gene            1598..3343
                     /locus_tag="VIL39_10055"
     CDS             1598..3343
                     /locus_tag="VIL39_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5481812.1"
                     /translation="MPPVKTFGERIADALVEDGLLTAQQVEELLEQQKKEGTRLLKLI
                     TEKAYVSDQDMAVSMGRVLNTPPINLARLTIPLEVADLVPREMAHNHKVVPVARLENR
                     LFLAMADPLNVLAIDDVRRITKLEVATLIAPEKAILDKLNHIDSSKSGSMEDIIQDAQ
                     KKGDVDADGEADSLEVSKDAVEEVNLDQLAASSGEAPVIKLANLILVQAVKDRASDIH
                     LEPFEKNLRLRYRVDGVLMDATPPPKQMQLALVSRFKIMSSMDIAERRLPQDGRMRIR
                     VGGKDYDLRVSIMPTVHGEKVVLRLLDKSNLSASIDKLGLDPDTFKQVKEAVDAPHGL
                     ILVTGPTGSGKTTTLYSALNELNSPIYNIVTVEDPVEFQVPGINQVPVKKEIGLTFAN
                     ALRSILRQDPDIIMIGEIRDTETAEIAIEAALTGHQVLSTMHCNDAPGAIARLDDMGI
                     APFLISSSVILACAQRLMRRICSHCKEPVTYPAKMFQDLGIDPSIFDGATLYRGRGCD
                     RCKNSGFSGRMAIIEAMTITDEIRKLIIARASTREMGKIAIGQGMRTLRMVALDRARE
                     GISTLEQVLVLTSAH"
     gene            3373..4578
                     /gene="glp"
                     /locus_tag="VIL39_10060"
     CDS             3373..4578
                     /gene="glp"
                     /locus_tag="VIL39_10060"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5481813.1"
                     /translation="MLEFEAALAQVLAAVPAPTNENVPLSEAAGRVLAGQVRSPIDLP
                     AFDNSAMDGYGLRAADVAAAKPDLPVRLRLAGRIAAGDVFAGEVMAGTCVRLFTGSPL
                     PPGADAVVMQETTRVEPNAADEVLILAPAAPGENVRSHGEDVKRGSTLGDAGELLTAG
                     RLGLLAAVGLASVRVGRQPVVGLLATGSELQEPGQPLAPGRIYESNRLALAALIMRAG
                     AVPRTFPLVADDLAAIRLALADACNQCDAVVTTGGVSVGEMDFIKRAFEQIGGELEFW
                     KVAIKPGRPFVFGRCRGKLLFGLPGNPVSALVTFLLLVRPALLRWQGAANISLPVVPG
                     VLAEPLVNDGGRRHFMRVRMDPAGKTYSAGVQASHVLSSVAAANGLVDVPANTTFTAG
                     TAVSVLRWD"
     gene            complement(4615..>5595)
                     /locus_tag="VIL39_10065"
     CDS             complement(4615..>5595)
                     /locus_tag="VIL39_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481814.1"
                     /translation="DQRRDRPIEFSSPRSDEVTTNLNQLTSRKDGLKQLEEDLYRPLQ
                     SFSPKSSLDGVVAPIPHSPAAPAIQSKRVKDLLERRKNWIFMRPEDLVAEPTVEDILK
                     TPDFGTDGREKPDPRSMEQYYHRLSTKRPAANRLDQSKEDDMFGSVRKSKSGDEMGAS
                     DDSSLPYGLKESVEALKNKFEPDKAGGPFAQSETRSSFSDPFGLGDNTQSKEQMLEHK
                     KFIDGFRTQVNNGWQPPAVAGSFSPSAGFAAAAQPMSSPAAGLSGSSSPAPHHGLDAQ
                     NDVLHPMLGPAGLPDVNARALGQSRPDLALPTVEAPKNVAPTFTAPKRAF"
BASE COUNT         1067 a         1699 c         1755 g         1074 t
ORIGIN      
        1 tgatagattt catagtttgg agggagaagt ggaagaagcg gacgaggttt gggacaggct
       61 gcttgcgggg tcagccgcgg tagccgtcca ggccaacgcc gaagcgaagg ccaacgagct
      121 gattcgagta tgatggctca cgagtattcc tttctttggg attaactaac aggcactgcg
      181 cggccgcatc cgacagaaaa caacaccctg ccgccatgac cgggtgaagg tcggatgttc
      241 gcgagtgatt cacagagcgt ttgagcatct gcattggggg cattcttcgg gtgacggcca
      301 ggagaacgca aacggaaaag tgaggcaaga gcaagaacta cgttttctgc aaaaagaatg
      361 gtcgccgccg gcttgggcac ctgagtcacc cgccgccacg tttgcggctt gccgctgtag
      421 tccgctacca tcaactgctc gcagcccttc gcgcacctcg ttcagcagct tgagtttggg
      481 gttgtggcgg cctttgggca ccggaaggtt tgccgggaac cggcttcctc cgcgagttcg
      541 gggaggcgtg gactgccggt ccgtgtcaaa acgagacaca agtgtccggt gattgggatc
      601 atcaggctgg cccgcaaagg agatttgcca tactgacggc aggtccctaa actggccgca
      661 agcttgctgc aaggaagccg tccggctgcc gaaatgtctc ggcgacagga ctgccctgac
      721 cggcacaata gaattcgcat ggccagcact ttaagtccga gtgaagaagc ccagttgggg
      781 cagacgattg agatgtttga agtaatcact caatcgcagc cgcatgacta tcagtcactg
      841 gagattctca aggaagctta ctcgaagctg aaccgcgaaa aggacgtgat aagcacgtcc
      901 aagcgcattg cccaggcgta cgtgcaaatg ggccagcttt cgtcggccat tctcgaatac
      961 gagaccatcc tgcagcgcta cccggacgat ccggacgtgc ggaccgcgtt gcagcaaatc
     1021 gaaagcaagg cgaacagctt ccccctcgaa accgccctgt cggcggacac cgtcctgctg
     1081 acccgagcgt ccgccgaggg cgacaaaccc cggaaagcgg ctagcaagga ggcgccagcg
     1141 gagatcgagg acgggcggcg tctgatgcac aagctttttg tcgagagcaa ggttatcacg
     1201 gctggggact ttgctctgtg ctggtccacg caggatctga ccctgccgcc gggcggcgtc
     1261 atggagccgt ttatccaggt tttgtccgac aagggcattc tgccagtgga caagtcgctg
     1321 aagatcttat gcgacaagtc gcggttcgct ttcctcccgc tcaacagcta cgacattgat
     1381 ctggagttag cgcggaagtt cccgcgcgta acctgccagc ggtggtgcgt gttgccgttt
     1441 gatcgcatga gcaaatcagt cctggtggcc acggcgaatc cattcaatca gcaggcggcc
     1501 aaggaattgg ccgaggccac cccgaaccgt ctgttgtggt acctggtgcc gccgtccgac
     1561 ctggtcgcca acgtccgcaa agccttccga taaacccatg cctcccgtca aaacatttgg
     1621 cgagcgcatc gccgacgcgc tggtggaaga cggcttgctg acggcccagc aggttgaaga
     1681 actgctcgag cagcagaaga aggaaggcac ccgcctgctc aagttgatca ccgaaaaggc
     1741 ctacgtcagc gaccaggaca tggcggtttc gatggggcgc gtgctcaaca cgccgccgat
     1801 caatctcgcc cgcctgacca tcccgctcga agtggcggac ctggtgccgc gcgaaatggc
     1861 gcacaaccac aaagtggtcc ccgtcgcgcg cctggagaac cggctgttcc tggcgatggc
     1921 tgacccgctc aatgtgttgg ccattgacga cgtgcggcgc atcacgaagc tggaagtggc
     1981 gaccttgatc gccccggaaa aggccatcct cgacaagctc aaccacatcg actcctccaa
     2041 gagcggcagc atggaggaca tcatccagga cgcgcagaag aagggcgatg tggatgcgga
     2101 cggagaagcc gacagcttgg aggtatccaa ggacgccgtc gaggaagtca atctcgacca
     2161 actggcagcc tccagcgggg aagccccggt cattaaactg gccaacctca tcctcgtcca
     2221 ggcggtcaaa gaccgcgcca gcgacatcca tctcgagcct tttgagaaga acctgcggct
     2281 gcgctatcgc gttgatggcg tcctgatgga cgcgaccccc ccgcccaagc agatgcaact
     2341 ggcgctggtc tcgaggttca agatcatgag cagcatggac attgctgagc gccgcctgcc
     2401 gcaagacggg cgcatgcgca tccgggtggg cggcaaggac tacgacctgc gcgtttccat
     2461 catgccgacc gtgcatggcg aaaaggtggt gctgcgcctc ctggacaaat cgaacctctc
     2521 cgccagcatt gataagctcg gccttgaccc ggacacattc aaacaggtca aggaggcggt
     2581 ggatgcacca cacggattga tccttgtgac cggcccgaca ggctcgggca agaccaccac
     2641 tctctattcg gcgcttaacg agctgaacag cccgatttac aacatcgtca cggtcgagga
     2701 cccggtcgag tttcaggtgc ctggcatcaa ccaggtgccg gtgaagaaag aaatcgggct
     2761 caccttcgcc aacgcgcttc gctcgattct gcgccaggac cccgacatca tcatgatcgg
     2821 ggagatccgg gacaccgaaa cggccgaaat cgccatcgaa gccgcgctga ccggccatca
     2881 ggtcttgagc accatgcact gcaatgacgc tcctggcgcg attgcgcgcc tggacgacat
     2941 gggcatcgcg ccgttcctca tctcttcatc cgtcattctc gcctgcgcgc agcgcctcat
     3001 gcgtcgcatc tgctcccact gcaaggagcc ggtcacctac ccggccaaga tgtttcagga
     3061 cttgggcatt gacccgagca tcttcgatgg cgccaccttg tatcgcgggc ggggctgcga
     3121 ccgctgcaag aactccggct tttccgggcg catggccatc atcgaggcga tgaccatcac
     3181 ggatgaaatc cggaagctca tcatcgcccg tgccagcacg cgcgagatgg gcaagatcgc
     3241 cattggccaa ggcatgcgga cgctgcgcat ggtggcgctg gaccgcgccc gcgagggcat
     3301 ctccacgctg gaacaggtgc tcgtgctgac ttcggcccat tagcggggcg gaggctcgcc
     3361 tcgcttttcc ctatgctcga atttgaagca gccctggcgc aagttctcgc ggcagttcct
     3421 gccccgacga acgaaaacgt cccgttgagc gaggcggcgg gccgcgtgct ggccgggcaa
     3481 gtccggtcac ccattgacct gccggccttc gataattcag cgatggatgg ctatggtctg
     3541 cgcgctgccg acgtcgccgc tgccaaaccc gatttgccgg tgcgcctgcg cctggcggga
     3601 agaatcgccg ccggagatgt cttcgctggt gaagtcatgg ccggcacttg cgtgcggctg
     3661 ttcaccggct ccccgttgcc gcccggcgcc gacgcggtcg tgatgcagga aacgacccgc
     3721 gttgagccca atgcggcgga cgaggttttg atcctggccc ccgccgcgcc gggagaaaat
     3781 gtccggtcgc acggggagga tgtaaagcgc ggctcgacgc taggtgatgc gggtgagctt
     3841 ctgactgccg gccgactcgg tctgctggcc gcagtcggct tggctagcgt tcgcgtgggg
     3901 cggcagccgg tggtgggcct gttggcaact ggctcggaat tgcaggagcc cggccaaccg
     3961 ctcgcgccgg gccggattta cgagagcaac cgcctcgcgc tggccgcgct gataatgcgc
     4021 gccggggcgg ttccccgaac gtttcctctc gtcgcggatg atctcgccgc gatccgcctg
     4081 gccctggccg acgcttgcaa ccaatgcgat gccgtcgtga ccaccggcgg cgtttcggtg
     4141 ggtgaaatgg atttcatcaa gcgagccttc gagcaaatcg ggggcgagtt ggagttttgg
     4201 aaggtggcga tcaaacccgg ccggccattt gtatttggcc gctgtcgggg aaagctgttg
     4261 ttcggcttgc ccggcaaccc agtctctgcg ctggtcacgt ttctgttgct ggtgcgtccc
     4321 gcgctgttgc gatggcaggg cgcagccaac atttcgttgc cagtcgtgcc gggtgtgctg
     4381 gctgagccgc tggtcaatga cggcgggcgc cggcacttca tgagggtcag gatggatccc
     4441 gccggcaaga cgtattctgc cggcgttcaa gcctcgcatg ttctaagctc agtggccgca
     4501 gccaacggct tggtggatgt gccggccaat acgacgttca ctgcggggac ggcggtgtca
     4561 gtgctacgct gggattaatc ctcaattcgg acttcgactc gggccgcagg ccctctaaaa
     4621 tgcccgcttc ggcgccgtga acgtgggggc aacgttcttc ggcgcctcga ccgttggcaa
     4681 agctaaatcc ggcctcgatt gcccaagcgc tcgcgcattc acatccggca gccccgctgg
     4741 gcccagcatg ggatggagga cgtcgttctg cgcatccagc ccgtgatgcg gggctgggct
     4801 ggaagagccg cttaagcccg ctgccgggct gctcatgggc tgggccgctg cggcaaagcc
     4861 tgccgacgga ctgaacgagc cggcaacggc gggcggttgc cagccattat tcacttgtgt
     4921 gcgaaatccg tctatgaact tcttgtgctc cagcatctgc tccttggact gcgtgttgtc
     4981 gccgaggccg aaaggatccg aaaagctgct gcgcgtctca ctttgggcaa acgggccgcc
     5041 ggccttatcg ggttcgaact tgttcttgag cgcctcgacg ctttccttga gtccgtaggg
     5101 caggctcgag tcgtcggacg cgcccatttc atcgcccgat tttgatttcc tgacggagcc
     5161 gaacatgtcg tcctctttcg attgatcgag gcggttagcg gcggggcgct tggtggaaag
     5221 gcggtggtag tactgctcca tggaccgagg atccggcttc tcccgtccgt ccgtcccaaa
     5281 atcgggagtc ttaaggatgt cctccaccgt cggctcggcg accaggtctt ccggacgcat
     5341 gaaaatccag ttcttgcggc gttccagcag gtctttcact cgcttgctct ggatggccgg
     5401 cgcggcaggg ctgtggggaa tcggagcgac cactccgtcc agcgaactct tgggcgaaaa
     5461 gctctgcagc ggccggtaga ggtcctcctc caactgcttc aggccatcct tcctgctcgt
     5521 caactgattc aggttggtgg tgacctcatc actcctgggt gaggaaaact caatcggccg
     5581 gtcgcgcctt tggtc
//
LOCUS       DATCGS010000492         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_488310, whole genome shotgun sequence.
ACCESSION   DATCGS010000492 DATCGS010000000
VERSION     DATCGS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488310"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VIL39_10070"
     CDS             complement(<1..1668)
                     /locus_tag="VIL39_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5481815.1"
                     /translation="MNFANPHMLWLLLAIPPALLAFFWWAGRTRQRLLTQFIQARLLP
                     TLTVGISATRQKISVGCLVLAVVCLILALARPQWGFDWEEMKQRGLDIVVAIDTSKSM
                     LAEDIAPNRLARAKLAALELMQRARADRLGLVAFAGSAFLQCPLTIDDAAFRQSVEAL
                     NVNIIPQGGTAIAEAIETALTAFKEGDNYKVLVLFTDGEDHDSGALEAAEKAAKDGMR
                     IFTIGIGTAEGELLRIKDAQGGSDYIRDEQGNVVKSHLNERLLQQIGGATEGGFYLPL
                     RGAKAIDTLYDQGLAKLPKAEHQEKFVRQYHDRYHWPLAVAIVLLLAEMLFPERKRES
                     KARAAATAPTLSGAKTLVFLLLVLPGAVRGSPASALREYKAGNYDQAVKEYEQLLKRK
                     SDDPRLHFNAGAAAYRNHQFEEASKQFNATLASPDLKLQGQAYYNEGNALFHLGEQSS
                     DPKKRTEAWEKALQDYQSTAKLNPQDADANYNYDFVKRKLEELKQQQQQKDQPNKIQP
                     SEAAKKAKAEADEAVRHREYAKALKIMENQLELDPTTSYYADYVQRLK"
     gene            complement(1665..2663)
                     /locus_tag="VIL39_10075"
     CDS             complement(1665..2663)
                     /locus_tag="VIL39_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5481816.1"
                     /translation="MTFGHPYFLLLLLLLPVLGWLKGKQGKPPAFVYSSVQLVRGILN
                     VTRTRSGAFLAALRWLILALLIIALAQPRLTKSETKISASGVDIAVALDMSGSMASED
                     FEVGSQRLSRLAMAKEVLKKFIDKRPNDRIGIVAFATQAYIAAPLTLDHEFLLQNLGR
                     LDLGTIDNSRTAIGSALSTAINRLRELKSKSKIVILMTDGQNNAGKVAPLTVAEAAQK
                     LGVKVYTIGVGTRGTAPMPVYFQGRKVGYRQEPVDIDEDTLQKIANMTGGKYYRADNA
                     QRFQAIYAEIDKLEKTEAEVKKFAHHNELFAWAISPGLGLLLLEVLLRHTLWRRLP"
     gene            complement(2660..3241)
                     /locus_tag="VIL39_10080"
     CDS             complement(2660..3241)
                     /locus_tag="VIL39_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481817.1"
                     /translation="MSVTNLHPQSVTAIAGTNALRDIKPPVDIPSGWAWVGWVLGALA
                     VAALIFWAWRYWQKRRSQVPSLPVIPPHIRAKQKLREALALIGQPREFCILVSDTVRW
                     YLEERFDFHAPERTTEEFLYELQGTNLLTPDQKDSLGEFLRRCDLVKFARYEPGQPEL
                     HDLHDSALRLVEETEPIMAVLEAVKTGEKVEKA"
     gene            complement(3244..>4005)
                     /locus_tag="VIL39_10085"
     CDS             complement(3244..>4005)
                     /locus_tag="VIL39_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5481818.1"
                     /translation="PFVKKFVEERELTLMLVVDASASGLFGSHAQSKRELAAEIASVL
                     AFSAIRNNDKVGLILFTDEVEKFIPPRKGRRHVLRVIREVLFFEPRHRGTDLNGALEF
                     LLRVTSHRAIAVVISDFIGSPAEARGRRRGKLRPQMMLLESLAQASFARLRQANRRHD
                     LVAVQITDRYELELPALGRLVLNDAETGEVVEINTGDERKRAAFAERQARAQTDLARL
                     FRSAGIDAIQLRTDQPYAAELGKFFETREKRRLRG"
BASE COUNT          745 a         1283 c         1156 g          821 t
ORIGIN      
        1 cttcagcctt tggacataat ccgcataata gctggtagtc gggtctagtt cgagttggtt
       61 ctccattatc ttgagggcct tcgcgtattc cctgtgccgt actgcttcat cagcctcagc
      121 ctttgccttc ttggctgctt cacttggctg aatcttattc ggttgatcct tctgttgttg
      181 ctgttgctta agctcttcga gcttccgctt aacgaagtcg tagttgtaat tcgcgtcagc
      241 atcctgcggg ttgagcttcg ccgtgctctg gtaatcctgg agggctttct cccatgcctc
      301 cgtacgcttc ttcgggtcgg aactctgctc cccgaggtgg aaaagtgcgt ttccttcgtt
      361 gtaataagcc tgcccctgaa gtttcaaatc cggcgaagcc agagtcgcat tgaattgctt
      421 ggaggcttcc tcgaactggt gattacggta agccgcggcg cccgcgttga aatgcaggcg
      481 cgggtcgtca ctcttgcgct tcagcaactg ctcgtattcc ttcaccgcct ggtcgtagtt
      541 cccggccttg tattcgcgca gcgcactcgc tggcgaacct cgcacggcac ccggcagcac
      601 tagcagcaga aatacgagcg tcttcgctcc gctaagagtg ggcgccgttg cagcagctct
      661 ggctttcgac tcccgcttgc gctccgggaa aagcatttcc gccaacaaca gcacaatcgc
      721 cactgccagc ggccaatgat accgatcatg gtactggcgc acgaatttct cctggtgctc
      781 agccttgggc agtttggcca acccctggtc gtagagcgtg tcgatggcct tggccccgcg
      841 caggggcagg tagaagccgc cttcggttgc gcccccgatc tgctgcagta gtctctcgtt
      901 caagtgcgat ttcacgacgt tgccttgctc atcccgaata taatcgctgc cgccctgcgc
      961 gtccttgatg cgcagcaact cgccttccgc ggtgccgatg ccaatcgtga aaatgcgcat
     1021 gccatctttg gccgccttct ctgcggcctc cagggcgccg gaatcgtggt cttccccatc
     1081 agtgaacagc accagcactt tgtagttgtc gccttccttg aaagcggtca gggctgtttc
     1141 aatcgcttcg gcgatggccg tgccgccttg ggggatgata ttgacgttga gcgcctcgac
     1201 gctctggcgg aaggccgcat cgtcaatggt caaagggcat tgcaggaacg cgctgccggc
     1261 aaacgccacc aggcccagcc ggtcggcccg ggcccgctgc atcaattcga gcgcggcgag
     1321 tttggcgcgc gccaaccggt taggcgcgat gtcctccgcc agcatgctct tcgaggtgtc
     1381 aatagcgacg acgatatcca gcccgcgctg cttcatctcc tcccagtcaa agccccattg
     1441 cggtcgcgcc agggccagga ttaggcaaac caccgccaac accaggcagc ccacgctgat
     1501 cttctgtcgt gtggccgaga ttccgacggt caacgtcggc aacagccgcg cctggatgaa
     1561 ctgggtcagc aatcgctgcc gggtgcgccc agcccaccag aagaaggcca gcagcgctgg
     1621 aggaattgcc agtagcagcc agagcatgtg gggattggca aagttcatgg gagcctcctc
     1681 cagagggtgt ggcgcagcag aacttccagc agcagcaacc ccaagccggg cgagatcgcc
     1741 caggcaaaca gctcgttgtg atgcgcgaac ttcttcacct ccgcctcggt tttctccagc
     1801 ttatcgatct cagcataaat ggcctgaaac ctctgggcgt tgtcggcgcg gtagtacttc
     1861 ccgccagtca tgttggcaat cttctgcagc gtgtcctcgt ctatatccac cggttcctgc
     1921 cggtaaccga cttttcgtcc ttgaaaatac accggcatcg gcgccgtgcc tctcgtgccg
     1981 acgccgatgg tatagacctt tacaccaagc ttctgggcgg cctcggccac agtcagcggc
     2041 gcgaccttgc cggcattgtt ctgaccatcg gtcatcagaa taacgatctt gctcttggat
     2101 ttgagttcgc ggaggcggtt gatcgcggtg ctgagagccg agccgatcgc agtgcggctg
     2161 ttgtctatgg tcccgagatc aagccggccg agattctgca agagaaactc gtggtcgagc
     2221 gtcagaggcg cagcaatata agcctgggtg gcgaaagcca ctatgccgat gcggtcgttc
     2281 gggcgtttat caatgaactt cttcaagact tctttcgcca tggccagccg gctaaggcgt
     2341 tgggacccga cttcgaagtc ctccgaggcc atgctgcccg acatgtcgag ggcaacagct
     2401 atatccacgc cgctggcgct gattttggtt tcgctcttgg tcaagcgcgg ctgcgccagc
     2461 gcgatgatca gcagcgccag gatgagccac cgcagcgccg cgaggaaggc cccggagcgc
     2521 gtgcgggtca cattgagaat cccgcgcacc agttgaacgg aagaatagac gaaggccggc
     2581 ggcttaccct gcttgccttt caaccagccc agcaccggca gcaacagcag caacagcaag
     2641 aaatatggat ggccaaaagt catgccttct caaccttctc acccgtcttg acagcttcca
     2701 gcacggccat gatcggttca gtctcctcca ccagccgcag cgcggagtcg tgtaaatcgt
     2761 gcagctcggg ctggcccggt tcgtagcggg cgaacttcac caggtcgcag cgccgcagga
     2821 actcgcccaa gctgtccttc tggtccggcg tcagcagatt ggtcccctgc aactcataca
     2881 ggaattcctc ggtcgtgcgc tccggcgcat ggaaatcaaa ccgttcctcc agataccagc
     2941 ggaccgtatc ggagaccagg atgcagaatt cgcgcggctg gccaatcagc gccagcgctt
     3001 cccgcagctt ttgcttcgcc cggatgtggg gcgggatgac gggaagcgac ggaacctgag
     3061 agcgccgctt ctgccagtag cgccaggccc agaagatcaa agccgcaact gcaagcgcac
     3121 ccaacaccca cccaacccac gcccacccgc tcggaatatc aaccggcggc ttgatgtcgc
     3181 gcaaggcatt cgtgcccgca atggccgtaa ccgactgggg atgaaggttc gtcacgctca
     3241 tgctcagccg cgcagccgcc gcttctcgcg agtctcgaag aacttgccca actccgccgc
     3301 gtacggctga tctgtgcgca actggattgc gtcaatgccc gcggagcgga ataggcgtgc
     3361 caggtcggtc tgggcgcggg cctgccgctc ggcgaaggcg gcgcgcttgc gttcgtcgcc
     3421 cgtgttgatc tcaactacct cgcccgtctc ggcgtcgttc agcaccagcc gccccagggc
     3481 gggcaattcc agctcataac ggtcggtaat ctggaccgct accaggtcgt gacgccggtt
     3541 cgcctggcgc agcctggcga acgaggcctg ggccagcgat tccagcagca tcatctgggg
     3601 ccgtagcttg ccgcgccgcc gacctctggc ctctgccggc gagccgataa agtcggagat
     3661 aaccaccgcg atggcccgat gcgacgtcac ccgcaacaga aactccagcg cgccgttcaa
     3721 gtccgtgcca cgatgacgcg gctcgaagaa cagcacctcg cgtatgacgc gcaggacatg
     3781 gcggcggcct ttgcggggcg gaatgaactt ctcgacctcg tccgtgaaca ggatcagccc
     3841 caccttgtcg ttgttgcgga tggccgagaa ggccagcacc gacgcgatct ccgccgccag
     3901 ctcccgtttg gactgcgcgt gcgagccgaa taaaccggag gcgctggcat ccacgaccag
     3961 catcagcgtc agctcgcgtt cctccacgaa cttcttaacg aatgg
//
LOCUS       DATCGS010000493         3283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_191884, whole genome shotgun sequence.
ACCESSION   DATCGS010000493 DATCGS010000000
VERSION     DATCGS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191884"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(544..1932)
                     /locus_tag="VIL39_10090"
     CDS             complement(544..1932)
                     /locus_tag="VIL39_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481819.1"
                     /translation="MNSSQGSTNRRDFIKTSGKAAAFSALANVALPPVHAAGSDQIQV
                     ALVGCGGRGGGAAANALAVKRGPIKLVAMADVFPERLTSALNALNRRFPSQVDVPPDR
                     QFLGFDAYRKAMDCLKPGDIAIFATPLAFRWVHFTYAIQKGLNVFMEKPLTADGPSSK
                     RMLALAQDASAKNLKVAVGLMSRHSRALQEMADRVHNGEIGEIVLQRGYRMHGPVGSF
                     LSPPKPADITHLLYQVKRFHSFIWAGGGCFNDFYIHIIDHQGWLKNQWPVKAQALGGR
                     HYKVSPEGVAYVDQNFDVYSVEYTYSDGTKFFFDGRCIAGCEEIYTSFLHGTKGAGIA
                     SKSGDCGLPSSLYKSQSATPADLIWESKVSPDERDPYQNEWNDFVDAIRDGKPYNEVK
                     RGVEASLVASMGRMSANTGRTITFDEMLNCEHEMAPGVDKFTMDSQAPLQADANGRYP
                     IPQPGIVTAREY"
     gene            complement(2367..>3283)
                     /locus_tag="VIL39_10095"
     CDS             complement(2367..>3283)
                     /locus_tag="VIL39_10095"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5481820.1"
                     /translation="PIAVQNVLTYLVGVLAVPETTGGVFDIGGPEALPYSDIIRIMAE
                     DLGLSRRWLIPVPVLTPRLSSYWIHLVTPLSHKIAIPLAEGLKNPVVCREDRITRLVP
                     QKLLTVREAIRAALSQVTARQVETNWSMAGPMPGDPDWSGGTVFRDTREVAIDAPAGA
                     VFRAVCRLGGQRGWHGANWLWIVRGWLDRLAGGPGLRRGRRDPEALSYGEALDFWRVV
                     TLEPDRCVSLRAEMRLPGEALLDFRIEPRNKGQCTCTLHQTALFRPRGLLGLIYWYAV
                     LPFHHLVFRGMLAGIQRDAVQIALLPQT"
BASE COUNT          625 a         1023 c         1020 g          615 t
ORIGIN      
        1 tcatccgccg gtacatgcct atattggcat aaatcagacg aagcgtcaaa cggcggccgc
       61 gggaaccgta tccctctgaa cgctccttac caaggctcgc gggcctccag cgccgtgtcg
      121 aaactactcc cgcgaacagg catcgcccat agcaggcgtc gtacaaggtt gactcgccgt
      181 gcaacggtcc tgcccttcgg cccggcgtta tccgtgacaa gccgacttgc cggaaaatgt
      241 catagtaacg gagctgcgag atcaagggct gattggctga gtgcagggac ggcaccatcc
      301 cgaactgtgg tggccgcgat taacctgccg gaaagtgccc ataccgctga tctgccggga
      361 tttccggcgc tcttgacggg ttactcccca caaacgccct tggcttccgg gtcacgtgtc
      421 gaggcgctcg cgcggggagc gggttgggtt tctccgacgc gacattcggg agccgctggc
      481 cacttcctgg ttttcaaggg ccggctgccc ccggttggag tgcggtaacc tcaaaacggc
      541 ttgtcagtat tccctcgcgg ttacgatgcc aggctgcggt ataggatatc tgccgttggc
      601 gtccgcttgg agcggggctt gagagtccat tgtgaacttg tcgacgcccg gcgccatctc
      661 gtgctcgcag ttgagcatct cgtcaaacgt aattgtccgg ccggtgttgg cggacatgcg
      721 gcccatgctg gcaaccaggc tcgcctccac gccgcgtttg acctcgttgt agggcttgcc
      781 gtcgcggatg gcgtccacaa aatcgttcca ctcgttctga tacggatccc tctcgtccgg
      841 gctcaccttc gattcccaaa tcaggtccgc gggggtcgcc gactgactct tgtacagact
      901 cgacggcaag ccgcaatcgc cgctcttgga agcgatgccc gcgcccttag tcccatgcag
      961 gaaactggtg tagatctcct cgcatccggc gatgcagcgc ccgtcgaaga agaacttggt
     1021 tccatccgag tatgtgtatt ccaccgaata gacgtcgaaa ttctgatcca cgtaggcgac
     1081 cccttcggga ctaaccttgt aatgccggcc gcccaacgcc tgggccttca cgggccattg
     1141 gttcttcaac catccctggt gatcgatgat gtggatatag aagtcgttga agcagcctcc
     1201 gcccgcccag atgaaactgt ggaagcgctt cacctggtag agcaggtggg tgatgtcagc
     1261 cggtttgggc ggggagagga aagagccgac gggaccgtgc atgcggtaac cacgctgcag
     1321 aacgatctcg ccgatctcgc cgttatggac acggtcagcc atttcctgca gcgcccggct
     1381 gtgacgcgac atgagcccga cggccacctt gaggttcttt gccgaggcgt cctgggcgag
     1441 cgcgagcatg cgtttactcg acgggccgtc ggccgtgagc ggcttctcca tgaagacatt
     1501 caagcccttc tgaatggcgt aggtgaagtg cacccagcga aacgccaggg gcgtggcgaa
     1561 aatggcgatg tcgccgggct tcagacaatc catcgccttg cgataggcgt cgaagccgag
     1621 aaactggcga tccggcggta cgtcgacctg actcgggaaa cggcggttca gcgcattcag
     1681 ggcgctcgta aggcgttccg ggaacacgtc cgccatagcg accagcttga tggggccgcg
     1741 cttgaccgct agtgcgttag ccgccgcgcc gccgccacgc ccgccacagc cgacgagcgc
     1801 tacctggatc tggtcgctgc ccgctgcgtg gacgggagga agcgcaacgt tggccaaagc
     1861 cgaaaacgca gccgctttac cgctggtctt gatgaaatca cgacggttgg tcgaaccttg
     1921 tgaagagttc atagctattc agtccctccg aggcttgcct ccacccggct gatcgaaggc
     1981 ctccgtggca agcgataata gtttaacccg gagcgcctct cgatgttgcc gcatgggtcg
     2041 gcgtcctccg agaggtccgc gcgggaatgg tgctttgacg gctttgcgcc ggatcgcgtt
     2101 ggcaaggccg cctgagccgc gttggtcgca gcccactgat gtgccccgtc cgccgactgg
     2161 agggggtagc cccgtttact ccccataaac gccccaggtt ccggcgatcc accttggggc
     2221 ggagtcggag ccccggcaac cgccgacttc agcgcacgcc agattcctgt cgacaacagc
     2281 cctactcgcc agcaaagacc tcctgacgcg aagccggttt cgcgacggga gtgcagggtt
     2341 ccgccgccag cggtgttggg caagggtcat gtctgaggca gcagagcgat ctgaacggcg
     2401 tcgcgctgga tgccagccag catcccgcgg aacacaaggt gatggaacgg gagcaccgcg
     2461 taccagtaga tcaaaccgag cagcccgcgg ggccggaaca aagctgtctg gtggagggtg
     2521 cacgtgcact ggcctttgtt acgtggttct attcggaaat cgagcaacgc ttcgcccggt
     2581 aatcgcattt cggctcgcaa cgagacgcaa cggtccggct ccaaggtgac gacgcgccag
     2641 aagtcgagcg cctcgccgta gctgagggct tcgggatcac ggcgaccgcg gcgcaatcca
     2701 gggcctccag ccagccggtc cagccagcca cggacgatcc agagccaatt ggcgccatgc
     2761 catccacgct ggccgcctaa gcggcatacg gcgcggaata ccgccccggc cggcgcgtcg
     2821 atggcaactt cgcgggtgtc gcgaaaaact gtgccaccag accaatccgg atcgccgggc
     2881 atcgggccgg ccatcgacca gttcgtctcc acctgacgcg ccgtaacctg gctgagtgcg
     2941 gcgcggatag cctcgcgaac ggtcaacagt ttctgcggga ccaaacgcgt gatccggtcc
     3001 tcacggcaca cgactgggtt ctttagtcct tcggccagcg gtattgcaat cttgtggctc
     3061 aatggggtca caagatgaat ccaataagaa ctcaagcgag gcgtcaggac tggaaccggt
     3121 atgagccagc gccgggaaag accgagatct tccgccatga tgcgaatgat gtcgctgtag
     3181 ggcagcgctt caggcccgcc gatgtcgaaa accccaccgg tggtttctgg gactgcaagc
     3241 acgccgacca ggtacgtgag cacgttttgg accgcgattg gct
//
LOCUS       DATCGS010000494         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_685667, whole genome shotgun sequence.
ACCESSION   DATCGS010000494 DATCGS010000000
VERSION     DATCGS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685667"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VIL39_10100"
     CDS             complement(<1..353)
                     /locus_tag="VIL39_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481821.1"
                     /translation="MFVGLGLFAFAGGTCAADGVVYQGQKGPGKGKHIVFLAGDEEYR
                     SEEGLPMLAKILAVRHGFKCTVLFPINPGDGTIDPLTLTNMPGLAALDSADLCVMGLR
                     FRELPDEQMKHFVDYL"
     gene            complement(380..1792)
                     /locus_tag="VIL39_10105"
     CDS             complement(380..1792)
                     /locus_tag="VIL39_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481822.1"
                     /translation="MKTSQSQLPSRREFIKTTGQFAAASALAGIAIPHVHAAQDNTIQ
                     LALIGCGGRGSGAVGNAMSAGGLVLGDDGGTKRAVGAGAGGPIKLVAMADIRQDKIDQ
                     SYTALKQALGDLIDVPSERKFLGFDAYRHAIDCLRPGDVAMLTTHAGFRAPHLEYAVQ
                     KGVNVFMEKDFAPDPGGIKRILRAGEAAERKNLKIGAGLMARHSSARQALIQKIRDGA
                     MGDIQLIRAYRMDSGYFMGPFPRGENELLWQLSPGHPYQFLWSSGGVFIELMIHQIDE
                     CFWIKDALPVSAHGVGGRFAGSTDCSQNLDSYSIEYTFADGAKALVTGRYIPNCLDDF
                     ATYLHGTKCAAKFSGNIHAPTSWLYKDQRTEQSNIAWRAAKETVNPWQAEWDVLLSAI
                     RNNKPHNEARRAALSNLGAIMGRAAVHTGKIITWEEAMASDFKFCPNVDELTANSPAP
                     VQADAQGRYPAPIPGKTVEI"
     gene            1953..>2490
                     /locus_tag="VIL39_10110"
     CDS             1953..>2490
                     /locus_tag="VIL39_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEY5481823.1"
                     /translation="MLKGIALRTNRFSGWVPRIHRVLFGLFLLQFILVWTRLWLPWPL
                     LGSARWPDGLLVVLTTAIVLASLTRQLPGQNVMLASTIIAFIAGAVQTLGALTAIPFG
                     PYVYTERIGQQLFYPLPWAVPLMWIAVTLASRGVARLTLRPWRKTQNYGFRLMGLTTL
                     LVVLLDLGLEPFATRVKHY"
BASE COUNT          430 a          756 c          804 g          500 t
ORIGIN      
        1 aggtaatcca cgaagtgctt catttgctca tccggcagct cgcgaaagcg caggcccatg
       61 acgcacaggt cggcggagtc gagcgccgcc aggccgggca tgttggtgag cgtgagcgga
      121 tcaatggtgc cgtcgcccgg attgatgggg aacagcacgg tacacttgaa cccgtgccgc
      181 accgcgagaa tcttcgccag catcggcaat ccctcttccg agcgatactc ctcgtcaccc
      241 gcaaggaaga cgatatgctt gcctttgccc ggccccttct ggccctggta aacgaccccg
      301 tccgctgcgc acgtcccgcc cgcaaaggcg aacagcccca gcccgacgaa catactgaca
      361 caccagctag tcttcatttc taaatctcca ccgtcttgcc ggggatgggc gccggatagc
      421 ggccttgggc gtcagcctgc accggcgcag ggctgttcgc ggtcagttca tccacgttgg
      481 ggcagaattt gaaatcggac gccatggctt cctcccacgt gatgatcttc ccggtgtgca
      541 ccgccgcccg gcccatgatg gcgccgagat tggaaagggc ggcacgccgg gcttcgttgt
      601 gcggtttgtt gttccggatg gcgctcagga gcacgtccca ctcggcctgc cagggattga
      661 cggtttcctt ggcggctcgc caggcgatgt tggactgctc ggtgcgctga tccttgtaga
      721 gccaggaggt cggggcgtgg atgttgcctg agaacttcgc ggcgcacttc gtcccgtgaa
      781 ggtaggtggc gaagtcgtcg aggcaattcg ggatgtagcg gccggtcacc agcgccttgg
      841 cgccgtcggc gaaagtgtat tcgatggagt agctgtccag gttctggctg caatcggtgc
      901 tgccggcaaa gcgcccgccg acgccgtggg cggatacggg taaggcgtcc ttgatccaga
      961 aacactcgtc aatctggtgg atcatcagct cgatgaagac gccgccggag gaccagagga
     1021 actggtaggg atggccgggg ctcagttgcc agagcagctc gttctcgccg cgcgggaacg
     1081 ggcccatgaa atagcccgaa tccatgcgat aggcgcggat aagctggatg tcgcccatag
     1141 cgccgtcgcg aatcttctgg atcagggcct ggcgcgcgga ggaatgccgg gccatgaggc
     1201 cggcgccgat cttgaggttc ttcctctccg cggcttcgcc tgcccggagg atgcgtttga
     1261 tgccgcccgg atcgggcgcg aaatctttct ccatgaacac gttgacgccc ttttgcaccg
     1321 catattccag gtgtggcgcg cggaagccgg cgtgagtggt cagcatcgcc acgtccccgg
     1381 gacgcaggca atcgatcgcg tgccgatagg cgtcgaaccc caggaacttc cgctccgacg
     1441 gcacgtcgat caaatcaccc aacgcctgct tcaacgcggt gtaggattgg tcaatcttgt
     1501 cctggcggat atcggccatg gcaaccaact tgatcggccc gcccgcgccc gcgccaacgg
     1561 ccctcttcgt gccgccgtca tcgcccaaga ccagtccgcc agccgacatg gcattgccca
     1621 ctgcgccgct gccccggccg ccgcagccga tcagagccag ttggatggta ttgtcctgtg
     1681 cggcatggac gtggggaata gctatgcccg ccaaagcaga tgcggcggca aactgaccag
     1741 tggtcttgat gaattcgcga cgtgagggaa gctggctttg gcttgtcttc atacaggcga
     1801 tgctggttcc gagggcgagt ccgtgtcaac catcaagtgg agaggggagg aaaggagtgg
     1861 tggagtggtg ggaggctaaa acgttggaag ccggagaatg tgtgtgaaat cgaagggccg
     1921 atggcatagc ttgtggacag atgaatcgag tagtgcttaa aggtattgcg ttgcggacta
     1981 accgcttttc gggctgggtg cccaggattc atcgagtgct gttcggcctg ttcctgctgc
     2041 aatttatcct ggtgtggacg cggctgtggc tgccctggcc gctgcttggc agcgcgcgct
     2101 ggccggatgg gctcctggtg gtcctgacga cggcgatagt gctcgcctcg ctcacccgcc
     2161 agttgcccgg ccagaatgtg atgctggcct ccactatcat cgcctttatc gccggcgcgg
     2221 tacagaccct cggcgcgttg accgctatcc ccttcggacc ttacgtctat accgagcgca
     2281 tcggccagca gctcttctat cccctgccct gggcggtgcc gctgatgtgg attgcggtca
     2341 cacttgcttc ccggggtgtc gcgcggctga cgctgcgtcc ctggcgcaag acccagaatt
     2401 acggcttccg gctgatgggg ctgaccaccc tgctcgtcgt cctgctggac ttgggcctgg
     2461 aaccgttcgc cacgcgtgta aagcactatt
//
LOCUS       DATCGS010000495         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_339413, whole genome shotgun sequence.
ACCESSION   DATCGS010000495 DATCGS010000000
VERSION     DATCGS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339413"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VIL39_10115"
     CDS             complement(<1..1279)
                     /locus_tag="VIL39_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5481824.1"
                     /translation="MSQRIVIDPVTRIEGHAKISIFMDDAGNVSDAEFHVVEFRGFEK
                     FCEGRPYSEMPGITPRICGICPVSHLLASAKAGDAIMAVSIPPAADKLRRLMNLGQFI
                     QSHALSFFHLSAPDFLLGWDTPPAKRNVFGLIGANTDLARAGIRLRQFGQEIIAVLGG
                     QKIHPAWAVPGGVRSPLTEGGRARIRAWLPEAYATTKIAFDLFKKTLDTHKKEIETFG
                     NFPSLFMGLVEPDGTWEHHGGKLRFTDSSGSIIADQLDPQKYYEYIGEGVQSSSYLKS
                     PYYLPLGIPDGMYRVGPLARLNVAEQMGTPKADAELKKFKKLGRGRSAVTSSFLYHYA
                     RLIEILASIERVEAAMDDADLLSEDLRADAGINSLRGVGVSEAPRGTLFHDYTVDRDG
                     LLKKVNLIVATGQNNLAMNQTVAQIARYYVRGNN"
     gene            complement(1282..1857)
                     /locus_tag="VIL39_10120"
     CDS             complement(1282..1857)
                     /locus_tag="VIL39_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014276573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY5481825.1"
                     /translation="MKTKPATSAQPLLRIATVWFGGCSGCHMSFLDLDEFLIELVSKV
                     EIVYSPVMDVKEYPEQVDVCLIEGAICNEDNLELLHKVRMRTKVLVSFGDCAVTANVP
                     AIRNQLGLGNAESVLQKAYIEEAQLNPGLPKEPGIVPALLERVMPVHEVVYVNYFLPG
                     CPPPAERIKALLVQVLNGEEPKLEGPQLKFG"
     gene            complement(1906..2625)
                     /gene="hoxU"
                     /locus_tag="VIL39_10125"
     CDS             complement(1906..2625)
                     /gene="hoxU"
                     /locus_tag="VIL39_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HEY5481826.1"
                     /translation="MAAKTLTIDGKQLSADESATILDAATDAGITIPTLCHLDGVYDV
                     GACRLCLVEVVGIAKLLPACTTKVGEGMDIKTDSERLRKYRRMTLELLFAERNHVCAV
                     CVANGHCELQKLAYSQGMEHVRYEYCFPKWEVDNTHPLFGMDHNRCILCSRCVRVCWH
                     IEGAGTKNVSGRGGKARIITDLNQPWGTSDTCTKCGKCVMSCPTGALFNKGSTVGEME
                     RDRTKLEFIVTAREKKQWIAE"
     gene            complement(2633..>4026)
                     /gene="nuoF"
                     /locus_tag="VIL39_10130"
     CDS             complement(2633..>4026)
                     /gene="nuoF"
                     /locus_tag="VIL39_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5481827.1"
                     /translation="GTLKGGKTKVQVGDPKDPFFTEQFPIVLANSGIVDPERIESYIA
                     AEGYQALHDVLREMTPKDVLDAILKSGLRGRGGAGYPTGLKWGTVAKTASQQKYVICN
                     ADEGDPGAFMDRSVLESDPHSVLEGMAIAAYAVGANQGFIYVRAEYPLAISRLQTAIK
                     QAKQHGLLGSGVFESPFNFNIDLRIGAGAFVCGEETALMASVEGKRGTPRPRPPFPAE
                     SGLWASPTLINNVETFANVAPIIRKGAEWFASIGTEKSKGTKVFALAGKINNTGLIEV
                     PMGTTLRQIVEKMGGGAPDGRKIKAVQTGGPSGGCIPTEALDTPVDYDSLSKLGSIMG
                     SGGMIVMDDTTKMVDVARFFMEFCMDESCGKCIPCRAGTVQMHGLLTKILARKATPRD
                     LQKLEELCEMVKSTSLCGLGQTAPNPVLSTLRYFRNEYTELLQKDTYGPNCYGGGATK
                     ESEATKPEAATLK"
BASE COUNT          707 a         1260 c         1236 g          823 t
ORIGIN      
        1 tattgttgcc gcgcacgtaa tagcgcgcga tctgggcgac ggtctggttc atcgccaggt
       61 tgttctggcc cgtggcaaca atgaggttga ccttcttcag caggccgtcc cggtccacgg
      121 tataatcgtg gaagagcgtg ccgcgcgggg cttcactgac gcccacgccg cggaggctgt
      181 tgataccggc atcggcgcgc aggtcttcgc tgagcaggtc ggcgtcgtcc atagcggctt
      241 ccacccgctc gatggaggcg aggatctcga tcagccgcgc gtagtggtag aggaaggacg
      301 aggtcaccgc gctgcgcccg cgtccgagct tcttgaactt cttcagctcg gcgtcggcct
      361 tgggtgtgcc catctgctcg gccacattga gccgcgccag cggcccgaca cgatacatgc
      421 cgtccgggat gcccaaaggc aagtaatacg gcgatttgag ataggacgag ctttgcaccc
      481 cttctccgat gtattcgtag tacttctgcg gatcgagctg gtcggcgatg atgctgccgc
      541 tgctatcggt gaaacggagc ttgccgccgt gatgttccca ggtgccatcg ggttccacca
      601 ggcccatgaa gagcgacggg aagttgccga aggtctcgat ctccttcttg tgggtgtcga
      661 gcgtcttctt gaaaaggtcg aacgcaatct tcgtcgtggc gtaggcttcg ggcagccagg
      721 cgcgaatgcg cgcgcgacct ccctcggtca gcggactgcg gacgccgccg ggcaccgccc
      781 aggcgggatg aatcttctgg ccgccgagga cggcgataat ctcctggcca aactggcgga
      841 gccggatgcc ggcgcgggcc aagtcggtat tggcaccaat gagcccgaac acattccgct
      901 tggccggcgg cgtgtcccag ccgagcagga aatcgggagc gctgaggtga aagaagctca
      961 gggcatggct ctggatgaac tggccgaggt tcatcagccg gcgcagcttg tccgcggccg
     1021 gggggatgct gacggccatg atggcgtcgc ccgccttggc cgaggcgagc agatggctga
     1081 cggggcagat gccgcagatg cgcggggtaa tgccgggcat ctcgctgtag ggacggccct
     1141 cgcagaactt ctcgaacccg cggaactcga cgacatggaa ctcggcgtcc gacacgttgc
     1201 cggcgtcgtc cataaaaatg ctgatcttgg cgtggccctc gatgcgggtc acgggatcaa
     1261 tgacgatacg ttgactcata attatccaaa cttgagctgc gggccttcga gtttcggctc
     1321 ttcgccgttg agcacctgca cgagcagggc cttgatgcgt tcagccggcg gggggcagcc
     1381 gggcagaaag tagtttacat atacgacctc atgcaccggc atcacccgct ccagcagcgc
     1441 gggcacgatg cccggctcct tgggcaggcc cggattgagc tgcgcctcct caatgtaagc
     1501 cttctgcaag acgctctccg cgttgcccag gccgagctgg ttgcggatgg cgggcacgtt
     1561 ggcagtcacc gcgcagtcgc cgaaggagac gaggaccttg gtccgcatgc gaactttgtg
     1621 gagcagttcc agattgtcct cgttgcagat ggcgccctcg atcaggcaca catccacctg
     1681 ctcgggatac tccttcacat ccatcaccgg gctatagaca atttccacct tactgaccag
     1741 ctcgatgagg aactcatcga ggtcgaggaa cgacatgtgg cagccggaac agccgccgaa
     1801 ccagactgtg gcgatgcgca agagaggctg cgcgctggtc gccggcttcg ttttcattag
     1861 ttgctttgta tccatgttag ttcagacacg ggcgggacac ccgtcctact ctgcgatcca
     1921 ttgtttcttc tcccgcgccg tgacgatgaa ttcgagcttg gtgcggtcgc gctccatctc
     1981 gcccacagtc gagcctttgt taaacagcgc gccggtgggg caggacatga cgcacttgcc
     2041 gcacttggtg caagtgtcag aggtgcccca cggctggttg aggtcggtaa tgatgcgggc
     2101 cttgccgccg cgcccggaga cgttcttggt gcccgcccct tcaatatgcc agcagacgcg
     2161 cacgcaacgg ctgcacagaa tgcagcggtt atggtccatg ccgaacagcg gatgcgtgtt
     2221 atcaacctcc catttcggga agcaatactc gtagcgcaca tgctccatcc cctggctgta
     2281 ggccagcttt tgcagctcgc agtggccgtt ggccacgcat accgcgcaca catgattccg
     2341 ctcggcgaac agcagctcca gcgtcatccg ccggtatttg cgcagccgtt cgctgtccgt
     2401 cttgatgtcc atgccctcgc cgaccttcgt ggtgcaggcg gggagcagtt tggcaatccc
     2461 gaccacttcc accaggcaga gccggcaggc gcccacatca taaacgccat ccaggtggca
     2521 gagcgtcggg atggtaatgc ccgcgtctgt agcagcgtcc aggattgtcg cgctctcgtc
     2581 ggcgctgagc tgctttccgt caatcgttaa tgttttggca gccatagaaa tttcacttca
     2641 gggttgcggc ttccggcttg gtggcttcgg attcctttgt ggcacctccg ccgtagcagt
     2701 tcggcccgta ggtatctttc tggagcagct ccgtatattc gttccggaag tagcgcaacg
     2761 tgcttagcac cggattgggc gcggtctggc cgaggccgca gaggctggtg ctcttcacca
     2821 tctcgcaaag ctcttcgagc ttctgcaggt cgcgcggcgt ggctttgcgc gcgaggatct
     2881 tcgtcagcag accatgcatc tgcaccgtgc cagcccggca gggaatgcat ttgccgcacg
     2941 attcgtccat gcagaactcc atgaagaacc gggcgacatc caccatcttc gtggtatcgt
     3001 ccatgacgat catgccgccc gagcccatga tggagccaag cttgctcagc gaatcgtagt
     3061 ccaccggcgt atcgagcgcc tcggttggga tgcagccgcc cgaaggcccc cccgtctgga
     3121 ccgctttgat cttgcggccg tccggcgcgc cgccgcccat tttctcaacg atctgccgca
     3181 aggtcgtgcc catcggcact tcgatcaggc cggtattgtt gatcttgccc gccagcgcga
     3241 acacttttgt gcccttgctc ttctcggtcc cgatgctcgc aaaccactcc gcgcccttgc
     3301 gaataatcgg ggccacgttg gcgaacgtct cgacgttatt gatcagcgtg ggggaagccc
     3361 acaagccgct ctccgccggg aacggcgggc gcggacgcgg cgtgccgcgt ttgccttcga
     3421 ccgaggccat gagcgccgtc tcttcgccgc aaacaaatgc gccggcgccg atgcgcagat
     3481 caatattgaa attgaagggc gactcaaaga cgccgctgcc cagcaagccg tgctgtttgg
     3541 cctgtttgat cgccgtttgc agccggctga tggccagcgg atactcggcg cgaacgtaaa
     3601 taaaaccctg attcgctccc acggcgtaag ccgcgatagc catgccttcc agcacgctgt
     3661 gcggatcgct ctccaacacg ctgcggtcca tgaacgcgcc gggatcaccc tcgtccgcgt
     3721 tgcaaatcac gtatttctgc tggctcgccg tcttggccac cgtcccccac ttgaggccgg
     3781 tgggataacc cgcaccgccg cgcccgcgca ggccgctctt gagaatcgcg tccaggacat
     3841 ctttcggggt catctcccgc aggacatcgt gcagggcttg gtaaccttct gccgcgatgt
     3901 aagactcgat gcgttcgggg tccacgatgc cgctattggc cagcacgatg ggaaattgct
     3961 cggtgaagaa cggatccttc ggatcgccga cctgcacctt ggttttgccg cccttgagcg
     4021 tgccga
//
LOCUS       DATCGS010000496        13664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_488731, whole genome shotgun sequence.
ACCESSION   DATCGS010000496 DATCGS010000000
VERSION     DATCGS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488731"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /gene="mutL"
                     /locus_tag="VIL39_10135"
     CDS             complement(<1..1304)
                     /gene="mutL"
                     /locus_tag="VIL39_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY5481828.1"
                     /translation="MNRIRLLPEQVANQIAAGEVIERPASVVKELVENSLDAQATRVM
                     VEVQAGGRSLVRVVDDGLGMSRDDALLCLERHATSKIRRAEDLAAITTMGFRGEALPS
                     IASVSRFTLTTRERADDSPEGTQIVISGGQILEVKAAGSAPGTTLEVRQLFFNLPARR
                     KFLRTEETESAHIQHYLMLAALAYPEVAFTFQRDGRLIWQLPAVKAGSDTASRLTALR
                     ERLRALYGGEQKLLTVDYSAEMPSPDDIEDPTGTPSSTLFRLWGFIGAPGVSRSTRDD
                     QHLFVNRRPVENRGLNFALLEGYHTALMKGRYPVCCLFLEIDPAAVDVNIHPAKREVK
                     FHREAEVRRLVAQAVRQTLVEFHGGKSESGKPKPAVQSPSSAAVEPAGEQPAQPAEQS
                     ALPNFPAALKPAPAQPPKPSTQQPPLRMGFSSAPPTPVLHVT"
     gene            complement(1489..2331)
                     /locus_tag="VIL39_10140"
     CDS             complement(1489..2331)
                     /locus_tag="VIL39_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481829.1"
                     /translation="MMNCGMGRCPELRLAGVRMLGLCLLLIAVLAAGCSSSGKKAGRA
                     GAPIRLPKPPVFLNGPMALLLTNMESFSAHAVLESGAPANGVELAAGELLGRRGNLLF
                     APGTGKNAGKRTRAEDFAFIWDVAANRGYVLNDPLQGYAPISYGRLFTNVATSTTLNN
                     AATESVAGHPCHPVEVTVAANDGTASVFRVWRATDLKGLPVRITGASDGTPLTLTLSK
                     VRLESVPNDLFQPPTGFTKYDSAEALINELTTRQASNKRRPTYQINDQDQFSTPETRS
                     PGRP"
     gene            2526..3392
                     /gene="mtnP"
                     /locus_tag="VIL39_10145"
     CDS             2526..3392
                     /gene="mtnP"
                     /locus_tag="VIL39_10145"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY5481830.1"
                     /translation="MSQPLIGIIGGSGLYHIEGFARQKWVNVKTPFGSPSDNFLTGNL
                     TGREVVFLPRHGRGHRIMPSELNHRANIWGMKKLGVAWIISVSAVGSLQAKYKPCDIV
                     LPDQFVDRTKQSSAHTFFGRGIVGHIPFADPICEELRRLLLKTARALKARVHDGGTYV
                     NMEGPAFSTRAESFTNRKLGHDVVGMTNLGEAKCSREAEIAYATMAMITDYDCWKVDE
                     AHVTVEMVIANLMKNMATAKAIIAKVLPEIPDAPNWPCHGALKNTIMTDRKCWPPKTT
                     RELEPILRKYLD"
     gene            3415..3792
                     /locus_tag="VIL39_10150"
     CDS             3415..3792
                     /locus_tag="VIL39_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY5481831.1"
                     /translation="MKNIIKPAKSAPAVGPYNHAVRIGSLLFCAGQIPIDPATGNLVA
                     GDIKAQTTRVLENVNAILEDQKLTFANVVKSTVFMTNLADFAGMNEVYAKYFTADFPA
                     RSTVQVAALPKGASVEIEVIAHY"
     gene            3874..5235
                     /gene="rmuC"
                     /locus_tag="VIL39_10155"
     CDS             3874..5235
                     /gene="rmuC"
                     /locus_tag="VIL39_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5481832.1"
                     /translation="MSPVAYLVIGLIAGAALGFLVGWLYRRGQAVAPDDRLANELRQQ
                     LTQREAELAKLRDQLATATSGQAAAEASRAAAERLVANQRELYEKSLRDAKEAQEKAL
                     ADLRAAFKALSVDTLRELQPQFIERVTETVAKLQETGKGDLTRREEAIKTLVKPLEEQ
                     LKIYQQRLQQTESAQSTTLGEVKKHLETLAQQSQSLSNETLQLRRVLSSNQARGRWGE
                     ETLRRVVEAAGMSAHCDFSEQTQAGDSKPDLIVRLPGDRLIIVDAKVPDLDFLNALDA
                     ADSTKRAEALAAHAGKLKATVKALADRDYPSQFPNALDYVVMFLPAESLFSAALEGDH
                     DLIVWAAGKHIMLATPASLIALLRSVSVSWQQHAQTENARAIAEAAQELYTRVCKFTE
                     HFEKIRAGLEKANAAFNDAAGSYERMVRPSGERLLKLRGDSQGKELTDIQPLSTALRL
                     PPG"
     gene            complement(5258..6364)
                     /locus_tag="VIL39_10160"
     CDS             complement(5258..6364)
                     /locus_tag="VIL39_10160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY5481833.1"
                     /translation="MPYQEKQLQDISKQFQIYGEILHAETFKIGHINETYSATYDQGG
                     MRVRYIHQKINKNVFKNPPVVMKNVMRVTTHIRHRLESQNARDITRRSLVVIPTRDGQ
                     SHYRSRDGEYWRTFVFVEGVQTYEAVESPDQALQAGRAFGEFQSLLVDLPGERLHETI
                     PAFHNTRKRFAALQQAILKDHFNRAQAAKREIEFALKHESLVDVILTAMAKKKIPERI
                     THNDTKFNNVMLDTVTGDAKCVVDLDTVMPGCALYDFGDMVRTTTSPTLEDERDLSKV
                     QMQMPMFKKLAEGYLATAGQFLTRAEKSFIAFSGKLITFEIGIRFLTDYLAGDTYFRI
                     HRSDHNLDRCRTQFKLVESIERQERAMQKYADSL"
     gene            6518..9391
                     /locus_tag="VIL39_10165"
     CDS             6518..9391
                     /locus_tag="VIL39_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY5481834.1"
                     /translation="MTADAFSAEDPTQGVRTCPAGADGSVTNQSPSVAKIKLFRALFR
                     GREDVYPRRFESRKTGRGGYQPACANEWARGLCDKPRVKCAKCANRRLLPVTDDVVRQ
                     HLSGQDAQGREFVMGLYPMLSDETCYLLAVDLDGDAWQEDTGALRETCKRLALPVALE
                     RSRSGKGGHLWFFFAEAIPASMARNMGSYLLTETMEGRPEIGLASYDRLFPNQDTLPK
                     GGFGNLIALPLQKKPRASDNSVFLDEQFEPYQDQWAFLSSARKIMRAQAESLAREAER
                     RGRITGVRPAFEEEEEDLPWTSPPSRRRKEPPIAEPLPKTLELVLGDQIYIAKEPLPP
                     ALRNRLVRLAAFQNPEFYRAQAMRLPTFGKPRVIHCAEDGPKYLALPRGCLGETQDLL
                     SSLGIEALLRDERFVGTPLDVRFHGELRPEQQLAANAMAAHDIGVLAATTAFGKTVLA
                     AWLIARRGVNSLILVHRQQLLEQWVERLATFLDVPAKSIGRLGGGQKTLAGRIDVALI
                     QSLVRKGVVHDCIGCYGHLVVDECHHLPASSFEQVARRAKARYVTGLSASITRKDGHQ
                     PIIFMQCGPVRYRVNARQQAALRPFTHHVLVRPTGFRAPDLAAADSRAEFQRLYEALT
                     ENPARNQLICRDVIAAAQAGRFPLVLTERTEHLRLLATQLSAAIPHVIILQGGMGRKH
                     LQAAMASLAQVPNDQGRALLATGRYIGEGFDEPRLDTLFLTLPVSWRGTIAQYVGRLH
                     RLHSGKREVQVYDYADLDVPMLARMFERRCAGYEAVGYTLLLPASAVPGWPVEVPLPI
                     DPEWKSDYSASVRRLVRDGVDVPLANLFVHAFRPMSPEADGVARARSASEAFLYRRLQ
                     TLPETTGLFRLNADLPIPFDTQGRMEVDLLCEELRLAIELDGGQHLANAEAYRRDRRK
                     DAALQEHGYFVLRFLVEDLGQGLDDILDVIQRAVAHQKRLAKR"
     gene            complement(9533..10519)
                     /locus_tag="VIL39_10170"
     CDS             complement(9533..10519)
                     /locus_tag="VIL39_10170"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY5481835.1"
                     /translation="MSPLVQVAIHASQFPENVRRDLLESLRTRRVNHKFHYDSIKQTQ
                     QWLALHQAFSPSRTDADCAATYDRSFAAAAARMGSRRVHLIGLGCGGGQKDTRLLKLL
                     QDSGRETFYTPADVSTAMVLVARQTATAVVPGENCFPLVCDLGSADDLPLVFDALPVS
                     GAARLIICFGMIPNFEPQVILPRLAGLMRPADHLLFSANLAPGPDYAAGMRQILPLYD
                     NALTRAWLMTFLLDLGVDANDGELRFTVEDDPVGSGLKRVAAYFRFQRAREIQVDAQR
                     FKFSAGEAIRLFFSWRHTPSLVRSLLGKHGLNVLDEWITKSGEEGVFLVSSA"
     gene            10617..11378
                     /locus_tag="VIL39_10175"
     CDS             10617..11378
                     /locus_tag="VIL39_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEY5481836.1"
                     /translation="MDFDISHLLNQWEYQPGQVVTRRFKAKDGREQIQLRLDLGLLQM
                     NVDGRPDGKRPFGYGSLFDYYRARLRKYRAAHDGSDEGFKLKAEDCVRLQLETLQYHN
                     RYICLLQLEDHARVIRDAERNLAVFDFVGKHTECEEMAWSLHQFRAQLLMILTRARAA
                     QALETEDYVMAVRHIEEGLEKIRAFYHEHGHAEAAETSGEVLSLEAWLEEIRAKRPLS
                     AQERLERALHEAINREDYERAAQVRDALRNLKPAD"
     gene            complement(11441..12004)
                     /locus_tag="VIL39_10180"
     CDS             complement(11441..12004)
                     /locus_tag="VIL39_10180"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5481837.1"
                     /translation="MDIKPWQIISSEPLGDFRIFSLRSDRAISPRTQQAHDVYVIDCP
                     NWVNVIAVTPDRHLVMIEQYRHGSTTVELEIPGGMIDPKDPSPTAAGLRELREETGYA
                     GENARLIGEIFPNPAIMSNTCYTVMVENCRCLHPVQFDHGEDLVTRLTPIADLPQLVA
                     SGKIRHSLVVVALYYFDLLQRRDRRAE"
     gene            12213..13277
                     /locus_tag="VIL39_10185"
     CDS             12213..13277
                     /locus_tag="VIL39_10185"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY5481838.1"
                     /translation="MTSIAKLLGDKAEFLLNFKNPKIAKERLHVPGPDVVDRLYAVSD
                     RNNRVLGNLQRIVTHGRLGGTGYLSILPVDQGIEHSGGASFAKNPDCFDPENIVRLAI
                     EGGCNAVASTYGVLGAVSRKYAHKIPFIVKINHNELMTYPNKFDQIMFGTVKQASDMG
                     AAAVGATIYFGSDQSARQIVEVAQAFAQAHEMGLATVLWCYLRNSAFKKDKDYHTSAD
                     LTGQANHLGVTIQADIIKQKLPENNGGFKALNVPGSSYGKFDERIYTELTSDHPIDLC
                     RYQVANCYMGRAGLINSGGESKGAGDLASAVTTAVINKRAGGMGLISGRKAFQRPMKE
                     GVGLLNAIQDVYLSKEVTVA"
BASE COUNT         2675 a         4143 c         4249 g         2597 t
ORIGIN      
        1 tgagttacat ggaggactgg ggtgggtggc gcagagctga agcccatgcg caagggaggc
       61 tgctgggttg atggttttgg cggttgcgcg ggcgcgggct tcagcgctgc cgggaaattc
      121 ggcagggccg actgctcggc cggttgagcg ggctgctcgc cggcgggttc gacggctgcg
      181 gaggatgggc tctggaccgc gggctttggc tttccggatt cagatttgcc gccgtgaaac
      241 tccaccagcg tctgccggac cgcctgggcc accaaccggc gcacctcggc ttcccgatga
      301 aacttcacct cacgcttcgc cgggtggatg ttcacgtcca cggccgcagg atctatttcc
      361 aggaacaggc agcagacggg gtagcgcccc ttcattaggg ccgtgtggta cccttccagc
      421 agcgcgaagt tcagcccgcg attctccacc gggcgacggt tcacgaagag gtgctgatca
      481 tcgcgggtgg agcgggagac cccgggcgcg ccgatgaaac cccagagacg gaagagggtt
      541 gaggaaggag tgccagttgg gtcctcgatg tcatccggtg agggcatttc ggcggagtaa
      601 tctaccgtca gcagcttctg ttcgccgccg tagagcgcgc gcagacgttc ccgcagggcg
      661 gtgaggcgcg aggcggtatc actgcccgcc ttgaccgcgg gcaattgcca gatcaggcgg
      721 ccgtctcttt gaaacgtgaa cgccacttcg ggatacgcca gcgccgcgag catcaggtag
      781 tgctgaatat gggcggactc cgtttcctcg gtgcgcagga acttgcgccg cgccggcagg
      841 ttgaagaaaa gctgcctcac ctccagagtt gttcccggtg cgctgcccgc cgctttgacc
      901 tccaggatct ggccgccgct gatgacgatc tgcgtgccct cgggcgaatc gtcggcgcgt
      961 tcgcgcgtgg tgagggtaaa gcggctgacg ctggcgatgc tgggcagggc ttcgccgcga
     1021 aagcccatgg tggtgatggc cgcgaggtct tccgcccgcc ggattttgct ggtggcatgg
     1081 cgctcgaggc agagcagtgc gtcatcgcgg ctcatgccca gcccgtcgtc caccacgcgc
     1141 accaggctgc ggccgcccgc ctggacctcc accatcaccc gcgtggcctg cgcatccagc
     1201 gagttctcga ccagttcctt caccacgctg gccgggcgct caatcacttc gccggcggcg
     1261 atttgattgg ccacttgctc gggcagcagg cggatgcggt tcatatgttc gttacatggt
     1321 taaataagtt acacggttac atgaggccac ccgccccgga cctcgggcac ccggtataac
     1381 gttgtaactc atgtaaccgt gcaacttatt taactttttc tggtcgctca tgccggggca
     1441 agctcatgac cggcacagac tgttggcatc ccattcccgc cgacctcact atgggcggcc
     1501 gggcgagcgg gtttccggcg tcgagaactg gtcctgatcg tttatctggt aggtcgggcg
     1561 ccgcttgttg ctggcctgcc gcgtggtcag ttcgttgatc aacgcttccg cgctgtcata
     1621 ctttgtgaat ccagtcggcg gctggaacag gtcgttcggc acggattcca gccggacttt
     1681 cgacagggtc agcgtgaggg gcgttccatc cgaggcgccg gtgatgcgga ccgggagccc
     1741 cttcaaatcc gtcgcccgcc aaacacgaaa gaccgatgcc gtcccatcat tcgccgctac
     1801 ggtcacttct accgggtggc aaggatgacc ggcgacgctc tccgtcgcag cattgttcag
     1861 ggtcgtgctg gttgccacgt tggtgaacag gcggccgtag gagatgggcg cgtaaccttg
     1921 caacggatcg ttcagcacgt aaccccggtt ggcagccacg tcccagatga aggcgaagtc
     1981 ctccgcgcga gtgcgtttgc cggcgttttt gccggtgccg ggcgcaaaca gcaggttgcc
     2041 tctccggccc aggagttcgc cggcggccag ctcgaccccg ttggcgggcg ctccgctctc
     2101 caacaccgcg tgcgcgctga aactctccat attcgtgagc agcaaggcca tcgggccgtt
     2161 gaggaatacg gggggctttg gcaggcgaat cggggcgccg gcgcggcctg ctttcttccc
     2221 cgagctggaa cagcctgcgg ccagaacggc gatcaacagg aggcataatc ccagcattcg
     2281 cacgccagcc agccgcaatt ccgggcagcg ccccattccg cagttcatca ttggcatatc
     2341 gttatggacc atatctgagc cgatccgttc taaaatcaag catataaacc acactccctc
     2401 gaagaagcag agcgggcagt actctgcccg ccgctgagtg ccaaccgtcg cttacccact
     2461 tctaactcgc gattcatccc ggttttcaat tttcaaatcc ccgctcaccc gctatgattc
     2521 ccggcatgtc tcaacccctt ataggcatta tcggcggcag tggcctctat catatcgaag
     2581 gctttgcccg gcagaagtgg gtcaatgtca agaccccatt tggttcgccc tcggacaatt
     2641 tcctcaccgg caacctgacg gggcgcgaag tggtgtttct gccccggcat gggcggggtc
     2701 accgaatcat gcccagcgaa ttgaatcacc gcgccaacat ctgggggatg aagaagctcg
     2761 gcgtggcgtg gattatcagc gtcagcgccg tgggctcgct ccaggccaaa tacaagccct
     2821 gcgacatcgt gttgcctgac cagttcgtgg accgcaccaa gcaatcctcc gcccacacct
     2881 ttttcggccg cggcatcgtc gggcacatcc cgttcgccga cccgatctgc gaggaactgc
     2941 ggcggctcct gctgaagacc gcccgggcgc tgaaggcgcg cgtccacgac ggcgggacct
     3001 acgtgaacat ggaaggtccg gcctttagta cccgcgcgga gtcgttcacc aaccgcaagc
     3061 tcggtcatga cgtggtgggc atgaccaacc tcggggaagc caaatgctcc cgggaagccg
     3121 aaatcgccta cgcgacgatg gcgatgatca ccgactacga ctgctggaag gtggacgagg
     3181 cgcacgtcac ggtggaaatg gtgatcgcca acctgatgaa gaacatggcg acggccaaag
     3241 ccatcatcgc caaggtgctg ccggagattc ccgacgcgcc caactggcct tgccatggcg
     3301 cgttgaagaa cacgatcatg accgacagga aatgttggcc gcccaaaaca acacgggagc
     3361 tggagccgat cttgcgcaag tatctggatt aagtaagatc gcactgcaat gcctatgaag
     3421 aatatcatca aaccagccaa atccgctccc gcagtcgggc cttacaatca cgccgtgcgc
     3481 attggcagcc tgcttttctg tgccggccaa attcccattg atccggccac cgggaatctc
     3541 gtcgcaggcg acatcaaggc gcagaccacg cgcgtcctgg aaaacgtcaa cgccatcctt
     3601 gaggaccaga agctgacctt cgccaacgtc gtcaagagca cggtattcat gacaaacctg
     3661 gcggacttcg ccgggatgaa cgaggtctat gccaaatact tcaccgcgga ctttccggcg
     3721 cggtccaccg tccaggtcgc cgcgctgccg aaaggggcga gcgtcgagat tgaagtgata
     3781 gcgcactatt gagttggcga ttggcaatta gcgccatgcc gatggtggtg ggccgccgat
     3841 gccactctcc aatcactaat catctgagat tccatgtccc ctgtagccta tttggtgatt
     3901 ggcctgattg ccggggccgc gctcggcttt ctggtcgggt ggctctatcg ccggggccag
     3961 gccgtcgcgc cggatgaccg gctcgccaac gaactgcgcc agcagttgac gcagcgcgag
     4021 gcggaacttg ccaaactgcg ggaccagttg gccacagcca ctagcgggca agcggccgcg
     4081 gaggcgagcc gggcggcagc ggagagactc gtcgccaacc agcgtgagct gtacgagaag
     4141 tccctgcgcg atgccaaaga ggcgcaggag aaggcgctgg cggatttgcg cgcggcgttc
     4201 aaggcgttga gcgtggacac gcttcgcgag ctgcaacccc agttcatcga acgcgtgacc
     4261 gagaccgtcg ccaagctcca ggagacgggg aaaggcgatt taacccggcg cgaggaagcg
     4321 atcaagacgc tggtcaaacc gctggaggag caactgaaga tttaccagca gcgcttgcag
     4381 cagaccgaga gcgcccaatc caccacgctg ggcgaggtga agaagcacct ggaaacgctg
     4441 gcgcagcaga gccagtcgct ctcgaacgaa acgctgcagt tgcgccgcgt gctcagctcg
     4501 aaccaggcgc gcggacgctg gggggaagag acgctgcgcc gggtcgtcga ggcggcggga
     4561 atgagcgcac actgcgattt cagcgagcag acccaggcgg gcgacagcaa gccggacctg
     4621 atcgttaggc tgcccgggga ccggctcatc atcgtggacg ctaaagtgcc ggacctggat
     4681 ttcctcaacg cgctggatgc cgccgacagc acgaaacgag cggaggcgct ggcggcccac
     4741 gcgggcaaat tgaaagcgac cgtgaaggcg ctggccgatc gggattatcc cagccagttt
     4801 ccgaacgcgc tcgattacgt ggtgatgttc ctgcccgcgg aatcgctgtt cagcgcggcg
     4861 ctcgaaggtg atcacgacct gatcgtttgg gctgccggca agcacatcat gctagccacg
     4921 ccagcgtctc ttatcgccct gcttcgttcc gtcagcgtca gttggcaaca gcacgcgcag
     4981 accgagaacg cccgggcgat tgccgaggcc gcgcaggagc tctacacccg ggtgtgcaag
     5041 ttcaccgagc acttcgagaa aattcgggcc ggattggaga aagcaaatgc cgccttcaat
     5101 gacgcggccg gcagctacga acgcatggtg cgccccagcg gcgaacggct gctcaaatta
     5161 cgcggggact ctcaaggaaa agaactcact gacattcaac ccctaagcac cgccctgcgg
     5221 ctgccgccag gctaggcggc ctctggcgct tgggatctca gaggctgtcc gcgtatttct
     5281 gcatcgcccg ttcctggcgc tcgatagatt ccacgagttt gaactgcgtg cggcagcgat
     5341 cgagattgtg gtcggaccgg tgaatgcgga agtaggtgtc cccggccagg taatccgtca
     5401 ggaaacgaat gccgatttca aacgtaatga gcttgccgga aaaggcgatg aaggatttct
     5461 ctgcccgggt caaaaactgg ccggccgtag cgagataacc ctcggcgagc ttcttgaaca
     5521 tcggcatctg catctggact ttcgagaggt cgcgttcgtc ctccagcgtg gggctggtcg
     5581 tcgtgcgcac catgtcgccg aaatcataga gcgcgcaccc aggcatgacc gtgtcgaggt
     5641 ccaccacgca ttttgcgtcg ccggtgaccg tgtcgagcat cacgttattg aacttggtgt
     5701 cgttatgcgt gatgcgctcg gggatcttct tcttcgccat ggcagtgagg atcacatcca
     5761 ccagcgattc gtgttttaac gcgaactcaa tctcccgttt ggccgcctga gcccgattaa
     5821 agtggtcttt caggatggcc tgttgcaggg cggcaaaacg cttccgtgtg ttgtggaaag
     5881 cggggatggt ttcatggagc cgctcgccgg gcagatccac cagcaggctc tggaattcgc
     5941 caaaggcgcg gcccgcctgc aaggcttggt cgggcgactc caccgcctca tacgtctgca
     6001 ccccctcgac gaacacaaac gtccgccagt attcaccatc gcggctgcga taatgcgact
     6061 ggccatcgcg cgtcggaatg accaccagcg aacgccgcgt gatgtcccgc gcgttctgag
     6121 attcgagccg gtggcgaatg tgcgtcgtca cccgcatgac gttcttcatc accaccggcg
     6181 ggttcttgaa gacgttcttg ttgatcttct ggtggatgta gcggacccgc atgccgccct
     6241 gatcgtatgt ggcggagtag gtttcgttga tgtgaccgat cttgaacgtc tcggcgtgaa
     6301 ggatttcccc gtaaatctgg aactgcttgg agatgtcctg tagctgcttt tcctgataag
     6361 gcataagtat ttcgagcttc ttcgattatg gtagtttggg cagcggctga caatccgtat
     6421 ttcaacagag gcttgcgggg aacaattctg ccgtaacggc gctgccggtg cgcgcggcgg
     6481 tatcgacagg gctgcggggc tcaggtagct tgaaggcgtg actgcggatg cgttctcggc
     6541 ggaggatccc acgcagggtg taagaacctg cccggcgggc gccgacggct cagtcacgaa
     6601 tcaatcgccc tccgttgcca aaatcaagct gttccgagct ttgttccgcg ggcgcgagga
     6661 tgtctatcct cgtcggttcg agagccgcaa gaccggccgg ggtggctacc aaccggcctg
     6721 cgccaacgaa tgggctcgcg gcttgtgcga caagccccgc gtaaaatgcg ccaaatgtgc
     6781 aaatcggcgc ctcctgccag tcaccgacga cgtggtccgc caacacctgt ccggccagga
     6841 tgcccagggt cgcgaattcg tcatgggcct gtacccaatg ctctcggacg aaacctgtta
     6901 tttgctggcc gtcgatcttg atggcgatgc ctggcaggag gataccgggg cattgcgaga
     6961 aacctgcaag cggctggcac tgccggttgc actggaacgc tctcgctcgg gcaagggcgg
     7021 ccacctctgg ttcttcttcg ccgaggccat ccccgccagc atggcgagga atatgggctc
     7081 atatcttctc accgagacca tggaaggccg cccggaaatc ggcctcgctt cttatgaccg
     7141 gctgtttccc aaccaggata ccttgcccaa aggggggttc ggcaacttga tcgcgctgcc
     7201 gctgcagaag aagcctcggg cgtcggacaa tagtgtcttc ctggacgagc agttcgagcc
     7261 atatcaagac caatgggcgt ttctgtcttc cgcgcggaag atcatgcgcg cacaagccga
     7321 gtccctggcc cgtgaggccg aacgccgggg acgcatcact ggcgtgcggc cggctttcga
     7381 ggaagaggag gaagatcttc cgtggacctc tccgccctcg cgtcggcgga aggaaccgcc
     7441 catcgcggag cctttgccca agacacttga actggtgctt ggcgatcaga tctacattgc
     7501 caaggagcct ctcccgccgg cactgcgtaa tcgtctggtg cgattggccg cgttccagaa
     7561 tcccgaattc taccgggcgc aggcaatgcg gctgcccacc ttcggcaagc cgcgcgtcat
     7621 tcattgcgcg gaggacggac cgaaatacct ggcgctgccc cggggctgcc tgggggaaac
     7681 gcaagacctg ctttcctctc tagggatcga ggcgctcctg cgcgacgaac gatttgtcgg
     7741 caccccactc gatgtgcgct tccacggtga attgcggccg gaacagcagt tggcggcgaa
     7801 cgccatggcg gctcacgata ttggagtcct ggccgccacc acggcctttg gcaaaaccgt
     7861 tctcgcggcc tggcttatcg cgcgccgcgg cgtcaatagc ctgattctcg tgcaccggca
     7921 gcaactgctg gaacaatggg tggagcgtct ggccaccttt ctcgatgtgc cggccaagtc
     7981 catcggcagg ctcggaggcg gccagaaaac tctcgccggc cggatcgacg tagccctcat
     8041 tcaaagcctg gtgcggaagg gtgtcgtgca tgactgtatc ggatgttacg ggcacttggt
     8101 tgtggacgaa tgccaccatc tcccggcgag cagctttgag caggtcgccc gccgcgccaa
     8161 agcacgctac gtcaccggcc tctccgcctc gatcacccgc aaagacggcc atcagccgat
     8221 cattttcatg caatgcggcc cggtgcgcta ccgtgtcaac gcccggcagc aggcggcgct
     8281 tcggcctttc actcaccacg tcctggtccg tcctacgggt ttccgtgcgc ccgacctagc
     8341 agcagcagat tcacgagccg agttccaacg gctctacgag gctttaaccg aaaatccagc
     8401 ccgcaatcaa ttgatctgca gggacgtgat cgctgcggcg caagccggtc gttttccgct
     8461 ggtgctgacg gagcgcaccg agcacttgcg gcttctcgcg acgcagttgt cggcggcgat
     8521 tccccacgta attattcttc agggcggcat gggcaggaag catcttcagg ccgccatggc
     8581 ctcgttggcg caagtgccga acgaccaggg gcgcgcgttg ctggccactg gccgctacat
     8641 cggcgagggc ttcgatgagc cgcggctgga cacgctgttc ctcacgctgc cagtatcgtg
     8701 gcgagggacc atcgcacagt atgtgggccg cttgcaccga ttgcacagcg gcaagcgtga
     8761 agtgcaggtc tatgattacg ccgatcttga tgtcccgatg ctggcgcgga tgttcgagag
     8821 gcgctgcgcc ggttacgaag cagtgggcta cacgctcctg ctgcccgcca gcgcggtgcc
     8881 cggctggccc gtcgaggtcc cattgcctat cgatcccgaa tggaagagcg attactccgc
     8941 cagcgtccgc cgcctggtcc gggacggggt ggacgttccc ctggcaaacc tctttgtgca
     9001 tgccttccgc cccatgtcgc ccgaagccga cggtgtggcc cgggcgcgca gcgcgagcga
     9061 ggcgtttctt taccgccgcc tgcaaacact gccggaaacc accggcctct tccgcttgaa
     9121 tgcagatctt ccaatccctt ttgatacaca aggccgaatg gaagtggacc tgctttgcga
     9181 ggaattgcgc ctggcgatcg aactggatgg cggacagcat ttagccaatg ctgaggctta
     9241 ccggcgggac cgtcgcaagg atgccgcgct ccaggaacat gggtatttcg tgctccgttt
     9301 cctcgtcgaa gaccttggcc agggcctgga tgacatcctg gatgtcatcc aacgtgcggt
     9361 agctcaccaa aagagactgg ccaagagatg aacgtccatt tcaccggtgc ccagaactga
     9421 atcgagatag aggggaggct tgcgcctcct ccccctccgc cggagctggt attactagcg
     9481 gggcttagtg cctaataatg cggagcttgg gagtttccta agggcattag ggctaagcgc
     9541 tggacaccag aaacacgccc tcctcccctg acttcgtgat ccactcgtcg agcacattca
     9601 aaccgtgctt gccgagcagg ctccgcacga gcgaaggagt gtgccgccac gagaagaaca
     9661 ggcgaatcgc ctcgccggct gaaaacttga accgttgtgc atcaacctgg atttccctcg
     9721 cacgctggaa ccggaaatag gcggccacgc gtttgaggcc gctgccgacc gggtcgtcct
     9781 cgacggtgaa gcggagttcg ccatcgtttg cgtccacgcc aaggtccagc aggaaggtca
     9841 ttaaccaagc gcgcgtcagg gcattgtcgt agagcggcag gatttgtcgc atgcccgccg
     9901 cgtaatcggg gccgggggcc aggttggcgc tgaagagcag atgatccgcg ggccgcatca
     9961 gtcccgccaa ccgcggcagg atgacttgcg gctcgaagtt cggaatcatg ccgaagcaaa
    10021 tgataagccg cgccgcgccg gagaccggga gcgcgtcgaa caccaagggc agatcgtcgg
    10081 cggaacccaa atcgcagacc aatgggaaac agttctctcc gggaaccacc gccgtcgccg
    10141 tctgccgcgc cacgagcacc atcgccgtgc tgacatccgc cggcgtatag aacgtttccc
    10201 ggcccgaatc ctgaagcagc ttcagcaacc gcgtgtcctt ctgtcccccg ccgcagccaa
    10261 ggccgatgag atgcacgcgt cgggagccca tgcgcgccgc agccgcggcg aagctgcgat
    10321 cgtatgtggc ggcgcagtcc gcgtccgtgc gcgaggggga aaaggcctgg tgcaacgcca
    10381 gccattgctg ggtttgtttg atgctgtcgt agtggaactt gtggttcacg cggcgggtgc
    10441 gcagcgattc cagcaaatcg cgccgcacgt tctccggaaa ctggctggcg tgaatggcta
    10501 cctggacgag cggtgacatg cgccagtgtt ttcgccgaaa ccagccggta ttacaagttt
    10561 tggatttccc tgggggaggc gttgacttgc cgggcacccg ccgccatatt tcgcttatgg
    10621 atttcgacat atcgcatctc ctgaaccagt gggagtacca gccgggacag gttgtcaccc
    10681 gcaggttcaa agcgaaggac gggcgcgagc aaatccagct tcggctggat ttgggcttgc
    10741 tgcaaatgaa cgtggacggc cggcctgacg gcaagcgtcc gttcggttat ggctccctgt
    10801 ttgattacta ccgggcccgg ctccgcaagt accgtgcggc ccacgacggc agcgacgagg
    10861 gcttcaaact gaaggcggaa gattgtgtca gactccagct cgagaccctg caatatcaca
    10921 atcgctatat ttgcctgctg caattggagg atcacgcccg ggtcatacgt gacgccgaac
    10981 gcaacctggc ggttttcgac tttgtcggca agcacaccga atgcgaggaa atggcctggt
    11041 cgttgcacca gtttcgcgcg cagctcctga tgattctgac ccgcgcccga gcagcgcaag
    11101 cgctggagac cgaggactat gtgatggccg tccggcacat cgaggagggc ctggaaaaga
    11161 tccgcgcgtt ttaccacgaa catgggcacg ctgaagcggc tgagacgagc ggcgaggtgc
    11221 tttcgttgga ggcctggctg gaggaaatcc gcgccaaacg cccactgtca gctcaggaac
    11281 gattggagcg cgcattgcac gaagcgatca atcgcgaaga ctacgaacgg gcggcgcaag
    11341 tgcgcgacgc attacgcaac ctcaagccgg ctgattagca gggtctcaac tccggcctgt
    11401 ttctgcggga agatccttcc caccggggca caaagatggg tcattccgcc cgccgatcgc
    11461 ggcgctgcaa gagatcaaaa tagtagagcg cgacgactac gagggagtgt cggatcttgc
    11521 ctgacgctac cagttgcggc aggtcggcga taggcgtcag ccgggtgacc aggtcctcgc
    11581 cgtgatcaaa ctggaccggg tgcaggcagc ggcaattctc caccatcacc gtgtagcagg
    11641 tgttgctcat gatggccggg ttcgggaaga tctcgccaat gaggcgcgcg ttttcgcccg
    11701 cgtaaccggt ttcctcgcgc agctcacgca ggcccgctgc cgtgggagag gggtctttgg
    11761 gatcgatcat gccgccggga atttccagct cgacggtcgt ggacccgtgc cggtattgct
    11821 cgatcattac caggtgccgg tcgggggtga cggcgatgac gttcacccag ttggggcaat
    11881 caatcacata aacgtcatgc gcctgttggg tgcgggggga gatggcccga tcggagcgga
    11941 ggctgaagat gcggaaatca ccgaggggct cggagctgat tatctgccag ggctttatgt
    12001 ccatggcctg agatcatacc ggccataata cattccagaa gcaaaaacta tatcgcacgt
    12061 gaacctgcaa atggaagtgg cgcggggctt gaattggagt agaatccatc cgccgttgtt
    12121 tcgtgccgtt ggcgcgccgc cagaaggcgc ctgcgcccgt ccacgcggca ttgccttttg
    12181 caattaaact gaaaacgaaa actggataaa ttatgacatc aatcgcaaaa ctgctcggcg
    12241 acaaagccga attcctgctt aacttcaaga acccgaagat tgccaaggaa cgcctgcacg
    12301 ttcccgggcc ggacgtcgtg gaccggctgt acgcggtctc tgaccgcaac aatcgcgtgc
    12361 tcggcaacct gcaacgcatc gtaactcatg gccggcttgg cggcacgggg tatctctcca
    12421 tcctgccggt tgatcaaggg atcgagcact caggcggggc gagcttcgcc aagaacccgg
    12481 attgttttga cccggagaac atcgtccggc tggcgatcga aggcggctgc aatgccgtgg
    12541 cttcgaccta cggcgtgctc ggcgcggtct cgcgcaaata cgcgcacaag attccgttca
    12601 tcgtgaagat caaccacaac gagctgatga cctacccgaa caagttcgac cagatcatgt
    12661 tcggcacggt gaagcaggcg tcggacatgg gcgccgcggc ggtgggggcc accatctatt
    12721 tcggctcgga ccagagcgcg cggcaaattg tcgaagtggc gcaggcattc gcgcaggcgc
    12781 acgagatggg cctggcgacg gtgctatggt gctatctgcg caacagcgcg ttcaagaagg
    12841 acaaggatta tcacacctcg gccgacctga ccggccaggc caatcatctg ggtgtgacca
    12901 ttcaggccga catcatcaag cagaagctgc ccgagaacaa tggcggcttc aaagcgctga
    12961 acgtccccgg ttccagctac ggcaagttcg acgagcgcat ctacaccgaa ctgaccagcg
    13021 accacccgat cgacctatgc cgttaccagg tggccaactg ttacatgggc cgcgccggcc
    13081 tgatcaacag cggtggcgag tccaagggcg cgggcgacct ggcaagtgcc gtcaccacgg
    13141 ccgtcatcaa caaacgcgcg ggcggcatgg ggctgatctc tggccgcaag gccttccagc
    13201 gtccgatgaa agaaggcgtc ggcctgctca acgccatcca ggacgtctat ctgagcaagg
    13261 aagtcaccgt ggcgtgagct gagttcgagc ccgcggctcg acgttaggat taagcattgg
    13321 aagtccgaat tgccggctgg cagttcggac ttctcttttt ggcgcaaggg gtgtttgaag
    13381 gatcagacag cctgacccgg ggaaggacgt gcgacacaat tctgcgccgc cgccggaata
    13441 tttccaaacg cccgaccacc tgtcgtttgc gtaaacgggt catttaatga gattgtaata
    13501 ttctgctgga ggcccgaggc aggctggaaa cttgacaacc gaccccttta tgctcaaacg
    13561 actgtcaatt cccaaaatga cccaagctat gggttgaccc ctttaagccg gctggttggg
    13621 ccatcgctgt tctaatctgc ccattttgtt cgtttggtga agat
//
LOCUS       DATCGS010000497         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_587194, whole genome shotgun sequence.
ACCESSION   DATCGS010000497 DATCGS010000000
VERSION     DATCGS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587194"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VIL39_10190"
     CDS             <1..563
                     /locus_tag="VIL39_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY5481839.1"
                     /translation="YLKAVFERQAEVAIEDLERLHTAIGDRAAIIQTNGTDFGTQNGP
                     FLNPAKYRDLFLPYQKRINGWIHRHSPWKTFMHCCGGIAPLLDAVVEAEFDILNPVQC
                     SAKGMDARALKQHYGDQLVFWGGGVDTQKTLPFGKPHEVRDQVRERIEIFGPGGGFVF
                     CTIHNIQANTPIENVLAMFEVVREYR"
     gene            685..1245
                     /gene="efp"
                     /locus_tag="VIL39_10195"
     CDS             685..1245
                     /gene="efp"
                     /locus_tag="VIL39_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5481840.1"
                     /translation="MASANDLRRGMAINYNGDICVVLDSQHRTPGNLRAFVQATLRSI
                     RSGRSSDVRFSSTEKIDVVPMMTRKMDFSYKDGEDFVFSDPETYETVTIAPEIVGDGK
                     NYLVENAQVTVTFVEDKAVTIELPSSVILKVADAPEGVRGDSANNVQKAIVMETGITI
                     QAPLFIKTGEKLKVDTRTGKYMERAN"
     gene            complement(1265..2314)
                     /locus_tag="VIL39_10200"
     CDS             complement(1265..2314)
                     /locus_tag="VIL39_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5481841.1"
                     /translation="MKKKSATARSGLLAGGNWIIDLVKMIDGYPQPEQLANIRSQSQG
                     TGGAPYNVLVDLARSGTPFPLFAAGLVGQDALGEHILDTCRQYKIDVQHLGTTPKAPT
                     SYTDVMTELGHGRRTFFHARGANALWRGEDLDFDKIPVRIFHLGYLLLLDALDEPDPR
                     FGTKAARLLARAQAAGVKTSADVVSENSDRFARIVNPALKHVDYCILNEIEAGKTTGF
                     KIRQPGGELDTVALRHAAGALLQQGVGELVVVHFPEGAFARTRKGDDFWQASLKLPPK
                     YIAGTAGAGDAFCAGVLYGLHEGWDLQRCLLTGVCIAAASLSDATCTSGVKSLSHSLA
                     LARKLGFADRVDAGD"
     gene            2501..>4115
                     /gene="ptsP"
                     /locus_tag="VIL39_10205"
     CDS             2501..>4115
                     /gene="ptsP"
                     /locus_tag="VIL39_10205"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY5481842.1"
                     /translation="MPEFQHTGEKSFRGIPVSTGVCRGKILVLGRARPSIAQRHVADE
                     ALAEEVNRLEKALVQTRQQILEIQRKVSEGMGAEEGSIFDAHLLVLEDRTLLDEIVRV
                     IQEQKVNAEHAFHTVAERYAATLSTVEDEYLRERAADMRDVTTRVLNNLLGLEEEAEL
                     RHLKEPCIIISHDLTPSNTAQLDKRKVLGFATDVGSKTSHTAIMARSLRIPAIVGLKD
                     VSGQLVTGQQALLDGLNGMLVVNPTDQTLFDYGQLILRQVTSQERLRDLLHEPAVTLD
                     GHRVLLNANIEQAADAEQVKANGAEGVGLFRTEYLFLNREHLPGEEQQYQDYRATAAA
                     LKPMPVVIRTLDLGGDKFLAHMQMPTELNPFLGWRAIRISLQERDIFRAQLRAILRAS
                     AEGNLKIMYPMISGLDELKQANALLEEYKAELRQENIPFDEHLEVGAMIETPSAVMVA
                     DALAKHVKFFSIGTNDLIQYSLAVDRMNEKIAHLYEPTHPAIVRLIKTTVDAAHRQKV
                     RVTVCGEMAGDPVLAPLLLGLGVDELSAAP"
BASE COUNT          849 a         1275 c         1263 g          728 t
ORIGIN      
        1 attacctcaa agctgttttc gagcggcagg ccgaggtggc tatcgaggac ctcgagcgcc
       61 tgcacacggc gatcggtgac cgcgcggcca tcatccagac caacggcacc gattttggca
      121 cgcagaacgg gccattcctc aaccccgcga aataccgcga cctgttcctg ccctaccaga
      181 agcgcatcaa tggctggatt caccgccact ccccttggaa gacgttcatg cattgctgcg
      241 gcggcatcgc gccgctgctg gacgccgtgg tcgaagccga gttcgacatt ctcaaccccg
      301 tgcagtgctc tgccaaaggc atggacgccc gcgcgctcaa gcagcactac ggcgaccaac
      361 tggtcttctg gggcggcggc gtggacacgc agaaaacgct tccctttggc aaaccgcacg
      421 aagtgcggga tcaggtccgc gagcggatcg agatcttcgg ccccggcggc ggcttcgtct
      481 tctgcacgat ccacaacatc caggcgaaca cgcccattga gaacgtcctg gccatgttcg
      541 aggtagtgcg ggaatacagg taggaagcgc acagcccaag tccccaaaat ccccaaaatc
      601 caggcttgca ttcgatccca gctcagatag tattcgcggc tcccgtcccg gaacccgggc
      661 ggtagttatt cagtttaaag aaatatggca tcagcaaacg atcttcgccg cggcatggcg
      721 atcaattata acggcgacat ctgcgtggtc ctggacagcc agcaccgcac gccgggcaac
      781 ctgcgcgcct ttgtgcaggc caccctccgc agcatccgca gcggccgatc ttccgatgtg
      841 cgtttcagct cgaccgagaa gatcgatgtg gtacccatga tgacgcggaa gatggatttc
      901 agctacaagg acggcgagga ctttgtcttc tccgatcccg agacttacga gacggtgacg
      961 atcgcaccgg agatcgtcgg cgacgggaag aactacctgg tcgaaaacgc ccaagtgacc
     1021 gtgacgtttg tcgaggataa ggcggtgacc atcgagctgc cgtccagcgt gatattgaag
     1081 gtggctgatg ctcccgaggg cgttcgcggc gactcggcaa ataacgtcca gaaggcgatc
     1141 gtaatggaaa cgggtattac catccaagcc cccctcttta tcaagaccgg cgagaagctc
     1201 aaggttgata cccgcaccgg caagtacatg gaacgggcga attagccagg tggctggatt
     1261 ctgttcagtc acccgcgtcc acccggtcag cgaaacctaa cttccgggcc agtgcgagcg
     1321 aatggctgag cgatttaacg cctgaagtgc aggtcgcatc cgagagcgac gcggcggcaa
     1381 tgcacactcc cgtcaacagg catcgctgca ggtcccagcc ttcgtgcaaa ccatacagga
     1441 ccccggcgca gaaggcgtca ccggcgcccg ccgtgccggc aatgtatttg ggcggcagct
     1501 tcaacgaggc ctgccagaaa tcatcgccct tgcgcgtgcg ggcgaaggca ccttcgggga
     1561 agtgaaccac caccaattca cccacgcctt gctgaagcaa cgcgccggcg gcatgccgca
     1621 gcgccaccgt gtctaactca ccgcccggct gccggatctt aaatccggtg gttttgcccg
     1681 cctcaatttc gttcaggatg cagtaatcca cgtgcttgag cgcagggttg acgatccggg
     1741 cgaagcggtc gctgttctcg ctcacgacgt cagcgctggt tttgacaccg gcggcctgcg
     1801 cccgggccag caagcgcgcc gctttggtgc cgaaccgggg gtccggttcg tccagggcgt
     1861 cgagcaggag caggtatccc agatgaaaga tgcgcacggg gatcttgtcg aagtccagat
     1921 cctcgccgcg ccaaagggcg ttggcgccgc gggcatgaaa gaacgtccgc cgcccgtggc
     1981 ccagctcggt catcacgtcg gtgtaggacg tgggcgcttt gggagtcgtg ccgaggtgtt
     2041 ggacgtcaat cttgtattgg cggcaggtat ccaggatgtg ctcgcccagg gcgtcttggc
     2101 cgacgaggcc ggcggcgaag agcgggaagg gcgtgccgga ccgggcgaga tcaaccaaga
     2161 cgttgtaggg cgcgccgccg gtgccctggg actggctgcg aatgttcgcg agttgttctg
     2221 gctggggata cccatcgatc atcttgacca ggtcaatgat ccagttcccg ccggccagca
     2281 ggccggagcg ggcagttgcg cttttctttt tcatgcggtc aggccgtcgt gtgtgtatta
     2341 gtgaatgaca aggagggtgc tgatggcaag ccatttcttc cggcaagcgc cttcggattc
     2401 cagactcggc ccgaccttac agccgccctg gccgaatctg ccgcttctct cttgcaaaga
     2461 attgcttttc aagcaagttc ggattagata agaatcaccc atgccagagt tccagcacac
     2521 gggagaaaag agcttccgag gtattccggt ctcgaccgga gtatgccgcg ggaagattct
     2581 tgtactgggc cgggcccgcc caagcatcgc gcaacggcac gtggcggacg aggctttggc
     2641 cgaagaggtc aatcggttgg agaaagctct ggtccagacc cggcagcaga ttctcgaaat
     2701 ccagcgcaag gtgagcgaag gcatgggggc cgaggaaggc agcatctttg acgcgcatct
     2761 gctggtgctc gaagatcgca cgctgctcga cgagatcgtg cgcgtgatcc aggagcaaaa
     2821 ggtcaacgcg gaacacgcct tccacaccgt tgccgaacgc tacgccgcga ccctgtccac
     2881 cgtcgaagac gagtatctgc gggaacgtgc cgccgacatg cgggacgtga cgacgcgcgt
     2941 gttgaacaac ctgctgggcc tggaggagga ggctgaactt cgccacctca aggagccttg
     3001 cattatcatc agccacgacc tgacgccctc caacaccgcg caactggaca agcgcaaggt
     3061 gttagggttc gcgacggacg tgggcagcaa gacctcgcat accgccatca tggcgcgttc
     3121 gctgcgcatc ccggcgatcg tgggtctgaa agatgtcagc gggcaattgg tgactggtca
     3181 gcaggcgctg ctggatggcc tcaacggcat gctggtggtc aatccaaccg accagacgct
     3241 gttcgattac gggcagctta tcctgcgcca ggtgacatcg caggagcggc tgcgcgacct
     3301 gctgcatgaa ccggccgtca cgctagacgg gcatcgggtg ctgctcaacg ccaacatcga
     3361 gcaggccgcc gatgccgaac aagtcaaggc caacggcgcg gagggggtgg gcctgttccg
     3421 caccgaatac ctgttcctca accgcgagca cctgccgggc gaagagcagc aataccagga
     3481 ctatcgcgcg accgcggccg ccctcaaacc gatgccggtc gtcatccgca cgcttgattt
     3541 gggcggcgac aagttcctgg cccacatgca gatgccgacc gagcttaacc ccttcctcgg
     3601 ctggcgcgcc attcgtatct ccttgcagga gcgggacatc ttccgggcgc agcttcgcgc
     3661 cattctgcgc gcaagcgcgg aaggcaacct caagatcatg tacccgatga tctcaggcct
     3721 cgacgaactg aaacaggcca atgcactgct cgaagaatac aaggccgaac tgcgccagga
     3781 gaacatcccc ttcgacgagc acctcgaggt cggcgcaatg atcgaaaccc cctccgccgt
     3841 gatggtggcg gacgcgctgg cgaagcacgt gaagttcttc agcatcggga ccaatgatct
     3901 gatccagtac tcgctggccg tggaccgcat gaacgaaaag atcgcccacc tctacgagcc
     3961 gacccatccg gccatcgtgc ggctgatcaa gaccacggtg gatgccgcgc accggcaaaa
     4021 ggtgcgtgtc accgtttgcg gcgagatggc cggcgacccc gtgctcgcac ccctgctcct
     4081 gggcctgggc gtggatgaat tgagcgccgc gccct
//
LOCUS       DATCGS010000498         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_92098, whole genome shotgun sequence.
ACCESSION   DATCGS010000498 DATCGS010000000
VERSION     DATCGS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92098"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VIL39_10210"
     CDS             <1..260
                     /locus_tag="VIL39_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481843.1"
                     /translation="TLTPPTLDLGLWTLETSDCDPGKPASAARLRSETTLPLKWIAAR
                     VQIGTAKGAKSVLHPLAQSQDQRKTASALEPCAQLEFQSTV"
     gene            complement(439..2739)
                     /locus_tag="VIL39_10215"
     CDS             complement(439..2739)
                     /locus_tag="VIL39_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEY5481844.1"
                     /translation="MNTKPRSYKKLWLSLISVMVLSFLVLGYYGTEIYRQAPPVPKRV
                     VTTDGTVLFTGQDIKDGQNVWQSIGGEEVGTIWGHGAYVAPDWSADWLHRESVWLLDH
                     WAAQSGGTTYEQLAPEIQAGLRQRLKTELRTNTYRAQTGDLVVSPVRAAAIKAIAEHY
                     TALFGNDPKLKELRNAYAIPANTIKTPERQELLNSFFFWAAWACSTERPDSQVTYTQN
                     WPPESLIDNRPTGSIVVWSVVSFVVLLAGIGAMVWYFAAQRHAQAGEEPDVPATDPLL
                     GLKPTASMQATLKYFWVVAALIVVQVGLGAVTAHYGVEGNGFYGIPLSQWLPYSVART
                     WHTQLGIFWIATAWLATGLFVAPAVSGYEPRGQKLGVNTLFVCLLVIVVGSLAGEWLG
                     VQQKLGMVSNFWFGHQGYEYVDLGRFWQIFLFAGLFIWLALMGRALWPAFKEPGPNRH
                     LLALFLVASTAIALFYGAGLMWGRQTHLAMAEYWRWWVVHLWVEGFFEVFATVVIAFL
                     FTRMGLLQTASATAAVMFSTVIFLAGGILGTFHHLYFSGTPTAVLALGATFSAMEVVP
                     LVLIGFEGYENLTLSRARPWVAAYKWPIYFFVAVAFWNLVGAGLFGFLINPPIALYYM
                     QGLNTTPVHGHAALFGVYGMLGIGLMLFCMRGLTQHRAWKSGTLTFSFWAINVGLALM
                     ILLSLLPVGLLQTWASVEHGMWYARSAEFMQTPTMNTLRWLRVVGDSIFALGVVGLGW
                     FVLGLKTGWSLASTSASREEPEHQMD"
     gene            complement(2860..3204)
                     /locus_tag="VIL39_10220"
     CDS             complement(2860..3204)
                     /locus_tag="VIL39_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5481845.1"
                     /translation="MQDTSSKPKGLAAALVAKAPELVNYQDSAVVSREIVKKPTGSVT
                     LFAFDEGQGLSEHTAPFDALAQVVEGEAEIIISGQPHRVLGGEMILMPAGQPHALKAT
                     KRFKMILTMIRS"
BASE COUNT          656 a          989 c          998 g          586 t
ORIGIN      
        1 caactttgac acccccaact ttggaccttg gactttggac cttggagact tcggactgcg
       61 accccggcaa gccggccagc gctgcccggc tgcggagcga aacgaccctg ccgctcaagt
      121 ggattgcggc gcgggtgcag atcggcacgg ccaaaggggc caagtccgtg ctccatcctc
      181 tggctcagag ccaagaccaa cgcaaaaccg caagcgccct cgaaccctgc gcccagctcg
      241 aattccaatc taccgtttga ccctttgtcg gctctgacgg ggagttcggg tttggaacgc
      301 cggctcgcag cgtatcaggg actcgggaaa gggtgggggc cggcgtttcc acagtctccg
      361 ccgctctcag actcgttcga aagtgccaat agtgaggaca cttgctcttg cttaacactt
      421 gctcagccgg gccggaagtc agtccatctg atgctccggt tcttctctgc tggcggacgt
      481 actggccaac gaccagccag tttttaggcc gagcacgaac catcccaagc cgaccacgcc
      541 gagcgcgaag atggaatcgc ccaccacccg aagccagcgc agcgtattca ttgtgggagt
      601 ctgcatgaac tcagcggagc gggcgtacca catgccgtgt tccacgctgg cccaggtctg
      661 aagcagaccc accggcagga ggctgagcag gatcatcaat gccagaccga cgttgatcgc
      721 ccagaagctg aaagtgagcg tgccggactt ccacgctcgg tgctgggtca gcccgcgcat
      781 gcagaacagc atcaggccga tgcccagcat gccatagacg ccgaatagag cggcgtgacc
      841 gtggacgggc gtggtgttca gcccctgcat gtagtagagg gcgattggtg ggttgataag
      901 gaagccaaac aggccggcgc ccaccaggtt ccagaaggcc acggccacga agaagtagat
      961 cggccattta taggccgcga cccacggacg cgcgcggctg agggtcagat tctcgtagcc
     1021 ctcaaatcca atcaacacca gcggcactac ttccattgcg ctgaaagttg cgcccagcgc
     1081 cagcacggcg gtgggcgttc cgctgaagta caggtggtgg aaggtgccga ggatgccgcc
     1141 tgccaggaag atcacggtgg agaacatcac ggcggcggtc gcgctcgcag tttgcagcaa
     1201 gcccatccgg gtgaacagga aggcgatgac gacagtggca aagacttcga aaaagccctc
     1261 cacccacaaa tgaaccaccc accagcgcca atactccgcc atggccaggt gcgtttggcg
     1321 accccacatc aaacccgcac cgtagaacaa agcgatggcg gtcgaggcga ccaggaagag
     1381 cgccagcaaa tggcggttag ggccaggctc cttgaatgcc ggccacaaag cccggcccat
     1441 cagcgccagc cagatgaata atccggcgaa gaggaagatc tgccagaagc ggccaaggtc
     1501 cacgtattcg tagccttggt ggccgaacca gaaattggaa accatgccca gcttctgttg
     1561 cacgcccagc cactcgccgg cgagcgagcc gacgacaatc accagcaggc aaacgaagag
     1621 cgtattcacg ccgagctttt gcccgcgcgg ctcatagccg gacacggcgg gtgccacaaa
     1681 gaggccggtc gccagccaag cagtcgcaat ccagaatatg ccgagctgag tgtgccaggt
     1741 gcgggccacc gagtagggca gccactggct cagggggatg ccgtagaagc cattgccttc
     1801 cacgccatag tgcgccgtca cggccccgag gcccacttgc accacgatca gcgcagcgac
     1861 cacccagaag tatttcaagg ttgcctgcat agacgcggtg ggcttgagtc cgagcagcgg
     1921 gtccgttgcc ggcacgtccg gttcctcgcc cgcttgggcg tgacgctggg cggcgaaata
     1981 ccacaccatc gcaccgatcc cggcaagcag gacgacaaag ctgactacgg accacactac
     2041 gatcgaaccg gtcggccggt tatcgatgag cgactcgggc ggccagttct gagtgtaggt
     2101 gacttggctg tcggggcgtt ccgtggagca agcccaggcc gcccagaaga agaaggagtt
     2161 cagcaattcc tggcgctcgg gggttttgat ggtattggcc ggaatagcgt aggcattgcg
     2221 gagttccttc agcttcggat cgttcccaaa gagcgcggtg taatgctccg ctatcgcctt
     2281 gatcgccgcc gcgcgcacgg gagaaaccac cagatcgcca gtctgcgccc ggtaggtatt
     2341 ggtgcgcagt tccgtcttca aacgctgtct cagccccgcc tggatttcgg gagcgagctg
     2401 ctcgtacgtc gtgccgccgg actgtgccgc ccagtggtcg agcagccaga cggattcacg
     2461 atggagccaa tcggccgacc agtccggcgc cacgtaagct ccgtggcccc agatggtgcc
     2521 cacctcttcg ccgccgatgg attgccagac attctggccg tctttgatat cctggcccgt
     2581 gaacagcacc gtgccgtcgg tcgttacgac gcgtttgggc accggcggcg cctggcggta
     2641 aatctccgtg ccgtagtaac cgagcacgag gaaggagagg accattacgc tgatcaggct
     2701 cagccagagt ttcttgtagg atcgaggctt tgtattcatc gtttgttttc agtttcttca
     2761 gtttttacgt tcgacgcgcg gtttgtggac ggcgcgcttc attggctgcc gggttgatgc
     2821 ccgcgcgttt ctcaatcggt tccgtcaggg agcttcacct caggagcgga tcatcgtcag
     2881 gatcatcttg aaccgcttgg tggccttgag cgcgtggggc tggccggcgg gcatcagaat
     2941 catttcgccg cccaacacgc gatgaggctg gccggagatg atgatttccg cctcgccttc
     3001 caccacttgc gccagcgcgt cgaaaggagc ggtgtgttcg ctcaggccct ggccttcatc
     3061 gaaagcgaag agcgtcacgc tgccggtggg tttcttgacg atctcgcggc tgacgaccgc
     3121 gctgtcctgg tagttgacga gttccggagc cttcgcgacc agggcggcgg ccagtccctt
     3181 gggtttggat gatgtgtctt gcataggttt gcttaatcaa agtagcacg
//
LOCUS       DATCGS010000499         3877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_587317, whole genome shotgun sequence.
ACCESSION   DATCGS010000499 DATCGS010000000
VERSION     DATCGS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587317"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VIL39_10225"
     CDS             complement(<1..412)
                     /locus_tag="VIL39_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5481846.1"
                     /translation="MFKASQHIAWAVVLGFTFASANAQDSPQLTLPEAHEAALLSHPQ
                     ISVADLKALAARQVTRQFRSAFFPTLYGSVMSVGTANDNTRLAAIGGLNNPSIFDRNA
                     EGLVLSQLITDFGRTANLTSSAKLRAQAEANNAQA"
     gene            complement(425..3604)
                     /locus_tag="VIL39_10230"
     CDS             complement(425..3604)
                     /locus_tag="VIL39_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5481847.1"
                     /translation="MSRFAIRYPYLIIVICLIVCVVGVTTLVRMPVDLFPPIKIPVVV
                     VATFFSGMPPEQIETDITGRFERFFTLASGIDHMESRSLPGVSLIKVYFQPGFNSDSA
                     VTSIANLAMANLRKLPPGTLPPVVLKFDASSLPVCLITLKGEGLKEAELRDLGQYNVR
                     NQVASVPGASVPQPFGGRYRQIMVYVDPLKLESHQLSVMDVVRTVNEANLILPAGDVK
                     IGPFDYNLYANSQVDDVRDINKLPLKTVGNASVLVGDVGKAEDASQIQYNVVRVDGQP
                     SVYLPVLKQGGDANTISVVDGIKEAVGHLLDVPKQMVTKVVFDQSVFVRNAIENLIHE
                     GAIGLVLTGVMILIFLGSMRATVAVFLSIPLSALAAFIALSFGDNTINAMVLGGLALA
                     FSRLIDNSVVVLENIFRHIELGESPEVAAERGGEEVALPVLAATLTTVVVFFPVLFLY
                     GVSRFLFTALALAVVLSLVASYVVALTVVPLFCAKLIKGHSVDAAGQHGEPKGWGQRF
                     NAWFNRKFKQMLDRYEGTLDLALLRPLATVLGITGLFVFSLALYPLIGKAYFPRTDPG
                     QFVINLKAATGTRIERTDKLVERVEQIVREIVPAKDLRIIVSNIGVTPGFSSIYTPNS
                     GPHTAFVQVGLQDGHHVSSFQYMDRVRTRLGRELPEVSAYFQTGGLVDAILNLGMPAP
                     LDIQVSGMNLEEAHRTATEIAQKVRALPGVSDVLVPQDIDYPALKLDIDRVRASELGL
                     NEKEVVGNVITALTSDGMIAPSFWVDPKSGNDYLLTVQYPENFVKNLADLASVPLRGP
                     HSTKPTRLDTVSQLRHFKAPTEVDHYQLRRIMDVYVSPAGEDLSQVLSGVEKLLRDTK
                     LPENIRVTVRGSANAMQVSFRSFGFGLLLSTLLVYLILVAQFESFLDPFIILLAVPTG
                     LTGVMLILFTTGTTLNVMSLMGVVMMVGIVVSNSILIVDFTRRLRKEGRPLREAVSLA
                     CRVRLRPVLMTSLATLIGLLPMATKLGMGSEAYAPLARAIIGGLAVSVVLTLFIVPAA
                     YYLLYRWQEARAEARRSRLLEDEATQPAA"
     gene            complement(3686..>3877)
                     /locus_tag="VIL39_10235"
     CDS             complement(3686..>3877)
                     /locus_tag="VIL39_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY5481848.1"
                     /translation="KVHSGELLHGDQHGVQSIPLGIAAQIPPIAARISAQKRELIALC
                     QSSDFSLEKLRAAIAKGKV"
BASE COUNT          835 a         1159 c         1161 g          722 t
ORIGIN      
        1 tggcctgcgc attattcgct tcggcttgcg cccgcagttt ggcgctgctg gtcaggttcg
       61 ccgtccggcc aaaatcggtg atgagctggg agaggaccaa cccctcggca ttgcggtcga
      121 agatgctggg gttgttaaga ccaccaatag ccgcgagccg cgtattgtcg ttggccgtgc
      181 ccacggacat gacgctgccg tatagggtgg ggaagaacgc cgaccgaaac tgccgggtga
      241 cctggcgagc cgccagcgcc ttcaagtcag cgacgctgat ttgcggatgg ctgagcagcg
      301 cagcctcgtg cgcctctggc agggtgagtt gcggagagtc ctgcgcgttg gcactggcga
      361 acgtgaagcc gagaacgacc gcccacgcga tatgctgaga tgccttgaac ataaaatgct
      421 tttgtcatgc tgctggttga gtcgcttcat cctccaatag gcgggatctc ctggcttcgg
      481 cgcgagcctc ctgccagcga tacaggagat aatacgccgc cggcacaata aagagcgtca
      541 gcaccaccga gacggccaga ccaccaatga tggcgcgcgc gagcggcgca taggcctcgc
      601 tgcccatgcc cagtttggtc gccatcggca gcaggccaat gagcgtcgcc agggaagtca
      661 tcagcaccgg ccgtagccgc acccggcagg ccagcgacac ggcttcacgc aacggccgcc
      721 cttcctttcg cagccgccgg gtaaaatcta caatcagaat gctgttggac accacgattc
      781 ccaccatcat caccacgccc atcaacgaca tcacgtttag ggtcgtgccc gtcgtaaaga
      841 ggatcagcat tacgcccgtc aaccctgttg ggaccgccaa gagaatgata aaagggtcca
      901 ggaacgactc aaactgcgcc accagaatca gatacaccag caacgtggag agcaggagcc
      961 cgaagccgaa actgcgaaac gacacctgca tcgcgttcgc cgacccccgg acggttaccc
     1021 ggatgttctc cggcaatttg gtgtcgcgta ggagtttttc cacgccgctc aggacctggc
     1081 tcagatcctc gccggccgga gacacgtaaa cgtccatgat gcgccggagc tggtaatggt
     1141 caacttcggt cggcgctttg aaatgcctga gttggctgac cgtatcgagc cgcgttggct
     1201 tggtcgaatg ggggccgcgt aacggcacgg acgccagatc ggccaggttc ttcacgaagt
     1261 tctcggggta ttggaccgtg aggagataat cgttgccgct cttgggatcc acccagaagc
     1321 tgggcgcaat catcccgtcc gatgtcaacg ccgtgatgac gttgcccaca acctcctttt
     1381 cgttcaagcc cagctcgctg gcgcggacgc gatcaatgtc taattttaac gccgggtaat
     1441 cgatgtcctg cggcactagc acgtcgctga cacccggcag ggcgcggacc ttttgagcga
     1501 tttccgtcgc ggtccggtgc gcctcttcga gattcatgcc gcttacctgg atatccaggg
     1561 gcgcgggcat gccaaggttc aggatggcat ccaccaggcc cccggtctgg aagtaggcgg
     1621 aaacctccgg caactcgcgc cccagcctgg tccgcacacg gtccatgtat tggaaactac
     1681 tgacgtggtg tccgtcctgt aatcccacct gcacaaaggc agtgtgcggg ccggagttgg
     1741 gcgtatagat cgaggaaaaa cccggggtga cgccgatatt ggaaacaatg atgcgcaggt
     1801 ccttcgcggg cactatttcg cgcacgattt gttcgacgcg ttcaaccagt ttgtcggtgc
     1861 gttcgatgcg ggtacccgtt gccgctttga gattgatgac aaactggcca gggtcggtgc
     1921 gcgggaagta ggctttgccg atcaatgggt aaagcgccag gctgaacaca aagaggccgg
     1981 tgatccccag caccgtggcc agcgggcgga gcagtgccag gtccaacgtg ccctcgtagc
     2041 ggtcaagcat ctgcttgaac ttgcggttga accacgcgtt gaaacgctgg ccccaaccct
     2101 tcggctcacc gtgctggccc gccgcgtcca ccgaatggcc tttgatcaac ttggcgcaga
     2161 acagaggaac gaccgtgagc gcgacgacat acgacgcaac cagggacagc accacggcca
     2221 gcgccagcgc tgtgaacagg aaccggctca caccatagag gaaaaggacg gggaaaaata
     2281 ccaccacggt tgtgagcgtc gcggccaata cgggtagcgc gacttcctcg cccccgcgtt
     2341 ccgcagccac ctcaggagat tctcccagtt cgatgtggcg gaagatgttc tccagcacca
     2401 ccaccgaatt atcaatcagc cgggagaacg ccagcgccag gccgcccagc accatcgcgt
     2461 tgatcgtatt atcgccgaag gagagcgcaa tgaaggccgc caatgcggac agcggaatgg
     2521 aaagaaacac cgcgaccgtc gctcgcatgc tgcccaggaa gatcagaatc atgacccccg
     2581 tcaaaaccaa cccgatggcg ccctcgtgga tcaggttctc aatggcattg cgcacaaaca
     2641 ccgactggtc gaacaccact ttcgtcacca tttgcttcgg cacatccagc aaatggccga
     2701 cagcctcctt gatgccgtcc acaaccgaga tcgtgttcgc gtccccgcct tgcttcagca
     2761 cgggcagata aactgaaggc tggccgtcca cgcgcaccac gttatattgg atttgcgacg
     2821 catcctcggc cttgcccaca tcgcccacca gcaccgaggc gttgcccact gtcttaagtg
     2881 gcagtttgtt aatgtccctc acgtcatcaa cctggctgtt cgcgtagagg ttatagtcga
     2941 acgggccaat cttcacatcg ccggcgggca ggatcaggtt cgcctcattc acggtgcgca
     3001 cgacatccat cacgctcagt tgatgcgatt cgagcttgag gggatcgacg tacaccatga
     3061 tctgccggta gcgcccgccg aagggctggg ggaccgaggc gccggggacg ctggcgacct
     3121 ggttgcgcac gttgtattgg cccagatcgc gcaattctgc ttcctttaag ccttcacctt
     3181 tcaaggtgat caagcagacc ggcaggctcg acgcgtcgaa tttcaaaacg accggcggca
     3241 aggtgcccgg cggaagcttc cgcaaattcg ccatggccag attggcaatg ctggtgaccg
     3301 cggagtccga gttgaagccg ggttggaaat agaccttgat caggcttacg cccggcagcg
     3361 accgcgattc catgtggtca atgccgctgg caagagtgaa aaagcgctcg aagcggccgg
     3421 tgatatccgt ctcgatttgt tccgggggca tgcccgagaa aaacgttgcc acgaccacga
     3481 ccgggatttt gatgggcgga aacaagtcca ccggcatgcg caccagagtg gtcaccccca
     3541 ccacgcagac gatgaggcag atgacgatga tcagataggg atagcgaatg gcgaagcggg
     3601 acatgtggct aaagggttaa aggagttaca cgagttgcat gagtttcatg ggttatatag
     3661 gcgaagcagg gccaatgcgg cgggctcaca cctttccttt ggctatggcg gcacggagtt
     3721 tttcaagcga gaaatccgag gattggcaga gggcaatcaa ctccctcttc tgggcggaga
     3781 ttcgagccgc gatgggcgga atctgcgcag cgatgcccag cgggatggac tggacaccgt
     3841 gctggtcgcc gtgcagcagt tcgccggagt gaacctt
//
LOCUS       DATCGS010000500         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_735929, whole genome shotgun sequence.
ACCESSION   DATCGS010000500 DATCGS010000000
VERSION     DATCGS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735929"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VIL39_10240"
     CDS             <1..1270
                     /locus_tag="VIL39_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY5481849.1"
                     /translation="LNGRFVLPDKTALSGEFCVRPSQGRLVLMGKDGHELARGLEIRL
                     APEGNATFTLRGVTIGINFHWERKEDQTFEGALRLLLNSDGTITAINDAGVEQYLKSV
                     ISSEMSAEAPIELLRAHAITSRSWLVAMLERQKRLKNVGGPARHGRESEEEVVRWYDR
                     EDHKHYDVCADDHCQRYHGITKIISKTAEEAVQNTRGVFLVYGGEICDARYSKSCGGR
                     SEVFESAWEDVSVPYLATVSDAAVKHPLICSEAAAERWVDSSPEAYCNTSDGKILRQV
                     LPSFDQETTDFYRWRVTYTQEELTALLQKRSGIDFGKIQQLQPLQRGPSGRIVRLRIV
                     GSRRVVVVGKELEIRRWLSPSHLYSSAFVVRTEEGPQGIPARFTLHGAGWGHGVGFCQ
                     IGAAVMAAKGFQAETIVQHYFRGADLKRLY"
     gene            1412..3259
                     /locus_tag="VIL39_10245"
     CDS             1412..3259
                     /locus_tag="VIL39_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEY5481850.1"
                     /translation="MTDNPTASSKTGRFLPRIGSPAYHVMLAGVAILILGPLGGISAA
                     FMNFSIGFFIGGQVLAGILGSTVTLPYGPEGRHGANYMQTMAASVAGMCAMAVLVQAM
                     VWLGLPEPPAWKLVLYFMSIGMFGVGIGMLYTPVLVDRMQLAYPSGFAVANILRALTD
                     KSLLKRSIAKLGGGMLTGYAGGLFSLNLASFGRFGLTQASIKALTNLSISTSTFGAGM
                     IVGARIAIPALVVALIGKWQTPYLVSIGWLKEGEPFRKIGFIIALGTILGAAVLDLAL
                     ILVQAARRFRQKSTEPAKPAEDWKRVNMLRLVLWVAFWGTATVLLGSQVLHQPVFFLA
                     VAVGLCFLFVLVNGISLGISDWNPISSAFVLTVFILASLGLRDPGVGLLCASILLIAC
                     SEGGDMQQDRSTGWRLGTNRIIQFRYQVIGIAMGAVLAVVLAKVFMHSYPVLRVDQFS
                     QQHAEGAQQWQSAMTYKIVGALQGITNPQPHVMKALQFGIFLGLLIEIARKLIKRNRR
                     YQQAVKTSRAGRTADFVLDAVVLPSPYASSFGGFVELPTVLWWTAGGVGASLYDLVQA
                     KLASRRPQPADGQLPSDMSTMSLVGGGLIAGDSLAALSVGLYGLIKTVL"
     gene            complement(3426..>3848)
                     /locus_tag="VIL39_10250"
     CDS             complement(3426..>3848)
                     /locus_tag="VIL39_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5481851.1"
                     /translation="GFERFYTMLEKYQKTTFIGHAQTWWANIDKNHSDQSVLYPKGKV
                     TPGGITDRYLSDYPNMYGDLSAGSGLNAFTRDEDYAREFIQRHQDKLMFGSDCNDLDG
                     KGSKCSGAGMIAAVRRLSPSKDIERKLLYRNAKRVFSI"
BASE COUNT          669 a         1177 c         1224 g          778 t
ORIGIN      
        1 tctgaatggc cggttcgtcc tcccggacaa gactgctttg agcggggagt tctgcgttcg
       61 gccaagtcaa ggccgccttg tgttgatggg gaaggatgga catgaactcg cccgcggact
      121 ggagatccgg ttggcgccgg aagggaacgc caccttcacc ctgcgcggcg tgaccatcgg
      181 gatcaacttc cactgggaac gaaaggagga tcagaccttc gagggagcac tgcggctcct
      241 tttgaactct gacggcacga tcactgccat caacgatgcg ggagtcgagc agtatctcaa
      301 gagcgtcatc tcgtcagaga tgagtgcgga ggctccgatc gagctgctgc gtgcccacgc
      361 gatcacctcc cgaagctggc ttgtggccat gctcgaacgg cagaaacggc tcaagaatgt
      421 cggcggcccg gcccggcacg gccgggagag cgaggaggag gtcgtccgat ggtacgaccg
      481 ggaggatcat aagcactatg acgtctgtgc cgatgatcac tgccagcggt atcacggaat
      541 cacgaagatc atctccaaga cagcggagga ggcagtgcag aacacccgcg gggtgttcct
      601 cgtctatggc ggcgagatct gcgatgcccg ttactcaaag tcctgcggcg gccggagtga
      661 agtcttcgaa agcgcgtggg aggacgtttc cgttccctat cttgcgacgg tgtccgacgc
      721 cgccgtgaag catccgctga tctgctctga agccgccgcc gagcggtggg tggactcttc
      781 gccggaagcg tactgcaaca cgtccgacgg caagatcctc cgacaggtcc tcccctcgtt
      841 cgaccaggag acgacggatt tctaccgctg gcgtgtgacc tatacgcagg aggaactgac
      901 cgcgttgctc cagaagcggt ccggcatcga ctttggtaag atccagcagc tccagccgct
      961 gcagcgtggt ccatcgggac gcattgtgcg gctccggatc gttggctccc ggcgggtcgt
     1021 cgtagtcggg aaggaattgg agatccggcg ttggctctcc ccctcgcatt tatacagtag
     1081 cgccttcgtc gtccggaccg aggaggggcc gcagggcatt cccgcgcggt tcacccttca
     1141 tggcgcggga tggggccatg gggtcgggtt ttgtcagatc ggcgcggcag tcatggctgc
     1201 gaagggattc caggcggaaa cgatcgtgca acactacttc cgcggagcag acttgaagag
     1261 gttgtattaa ccgaagtaat gagagacgcc gactgacgtc ggcggctacg ccctgtcggc
     1321 gaatcccgtg aaatgggata tactgaggcc atccgccttt tcctttccct atcacaccgc
     1381 atctcatacc ttccggcgaa tttgtcacaa tatgacggat aatccaacgg ctagttccaa
     1441 gaccgggcgc tttctgcccc ggatcggctc gcccgcctat cacgtcatgc tggccggcgt
     1501 ggcgattctg atcctggggc cgttgggcgg gatctccgcc gcgttcatga acttctcgat
     1561 tgggttcttc atcggcggcc aggtgctggc ggggattctc ggcagcacgg tcacactgcc
     1621 ctatggcccg gaaggccggc acggcgcgaa ttacatgcag accatggccg catcggtggc
     1681 cggcatgtgc gccatggccg tgctggtaca ggcgatggtc tggctggggt tgcctgagcc
     1741 gcctgcctgg aagctggtcc tctacttcat gtccatcggc atgttcggcg tcggcatcgg
     1801 catgctctac acgcccgtgc tcgtggaccg catgcaattg gcctatccat cgggcttcgc
     1861 ggtggccaac attctccgcg cgctgaccga caagagcctg ctcaagcgct ccattgccaa
     1921 gctcgggggc ggcatgctga ccggctacgc gggcgggcta ttctcgctca acctggcatc
     1981 gtttgggcgc ttcggcctca cgcaggccag catcaaggcg ctgacaaacc tgagcatctc
     2041 cacctccacg tttggcgctg gcatgatcgt cggtgcgcgc atcgcgattc ccgctctggt
     2101 cgtcgcgctc atcggcaaat ggcaaacacc ctatctcgtg agtatcggtt ggctgaagga
     2161 aggcgaaccg ttccgtaaga tcggcttcat cattgcgctc ggcaccattc tcggggcggc
     2221 cgtcctcgac cttgcgctga ttctggtcca ggcggctcgc cggttccgac agaagagcac
     2281 cgagccggcc aaacccgccg aggactggaa gcgcgtcaat atgctgcgcc tggtgttatg
     2341 ggtcgccttt tggggcacgg ccacggtgct cctgggtagc caggtgctgc accagccggt
     2401 gttcttcctc gccgtcgcgg tgggcctctg cttcctgttc gtgctggtga acggcatctc
     2461 gctgggcatc tccgactgga accccatctc cagcgccttc gtcctgaccg tcttcatcct
     2521 cgcatcgctg gggctgcgtg acccgggcgt gggcttgctc tgcgcgtcca tcctgcttat
     2581 cgcgtgcagc gaaggcggcg acatgcagca ggaccgctcg accggctggc gcctgggcac
     2641 caatcgcatc atccagttcc gttaccaggt catcggcatc gccatgggcg cggtgctggc
     2701 ggtggtgctg gcgaaagtgt tcatgcactc gtatccggtg ctgcgcgtgg accaattcag
     2761 ccaacagcac gccgaaggcg cccaacaatg gcaatcggcc atgacctaca agatcgtcgg
     2821 cgcgctccaa ggcatcacca atccccagcc tcacgtaatg aaagcgctgc agttcggcat
     2881 ctttcttggc ctgttgattg aaatcgcccg caagctgatc aagcgcaacc ggcgctacca
     2941 gcaagccgtg aagacctccc gcgcggggcg cactgccgat ttcgtgctcg atgccgttgt
     3001 gctgccgagc ccttacgcat cgtccttcgg cgggtttgtg gagttgccca ccgtgctctg
     3061 gtggacggcc ggcggggtgg gagcgtcttt gtacgacctg gtccaggcca aactcgcctc
     3121 gcgccggccg cagccggcgg atggacagtt gccgtccgac atgagcacga tgtcgctggt
     3181 gggcggcgga ttaatcgccg gcgattcgct ggccgccttg agcgtggggc tgtatggcct
     3241 gatcaaaacc gtcctgtgac atgagccaac aaatcgcccg cgtgcgtgca aaccgagcga
     3301 cgcagcaaaa gtgcagatcc tgatcagtaa cgagctgtcg ccggcatgaa ggctgcgcgg
     3361 tgagggcagc gctgcggctt gaggccgcgc cgtcgccacc gaacccttga aactggaggt
     3421 gggcgctaaa tgctgaacac ccgcttggcg ttccggtaga gcagcttgcg ctcgatgtcc
     3481 ttgcttgggg acaggcgccg gacggcggca atcatccccg cgccgctgca cttggagccc
     3541 ttgccgtcca gatcgttgca gtcgctcccg aacatcagtt tgtcctggtg gcgttgaatg
     3601 aattcgcggg cgtagtcttc gtcgcgcgtg aacgcgttga ggccggagcc ggcagacagg
     3661 tcgccgtaca tgttcgggta atcactcaag taacggtcgg tgatgccgcc gggggtgacc
     3721 ttgcctttag gatagagcac tgactggtcg ctgtggttct tgtcaatgtt ggcccaccag
     3781 gtttgggcgt gaccgatgaa cgtcgtcttc tgatacttct ccagcatcgt gtagaaccgc
     3841 tcgaagcc
//
LOCUS       DATCGS010000501         4601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_636674, whole genome shotgun sequence.
ACCESSION   DATCGS010000501 DATCGS010000000
VERSION     DATCGS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636674"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..999)
                     /locus_tag="VIL39_10255"
     CDS             complement(196..999)
                     /locus_tag="VIL39_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481852.1"
                     /translation="MNESVEKAPVAWQPLTLRGMAAFACAPLRRLLLVQFIVALLAAA
                     SVVWFLHHAWFPTIGEAISQLPPQGELRSGRLDWQGSTPSRLAEGQFLALVVDLDHAG
                     EARSPAQVQVEFGRTDCKVFSLFGYVQDAYPRSWVVAFNQSELGPWWGAWAPPLLAIA
                     AGLVVVSLMVTWACLATVYFLPAWLVGFFADRELSLCGSWRLAGAALMPGALFLCGAI
                     FLYGWGALDIVRLAVAAAVHLVMGWIYLLVSLLCLPRQPVATGEENPFA"
     gene            complement(1074..1526)
                     /locus_tag="VIL39_10260"
     CDS             complement(1074..1526)
                     /locus_tag="VIL39_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481853.1"
                     /translation="MQATRILPDLQCSLMCEEVRQEANGNFFLIGVTSFIRVPQLPVM
                     AFKLSVFNRWTAGVGQFVETSRFIAPDQTTVLRKGEVKFGLRDAGLHATNVTVFGQVE
                     FKVAGTYYVEVLVDDVMKLRYPVPVIVTPPPNQSPVPSAPPASQTPAP"
     gene            complement(1533..2459)
                     /locus_tag="VIL39_10265"
     CDS             complement(1533..2459)
                     /locus_tag="VIL39_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY5481854.1"
                     /translation="MPPANLIDGRGIARQLQGELVQRIAALKARGVSPGLAFVRVGED
                     PASKVYVGRKEKTCAELGIYSEMHVLPEATSEGELLGLLDRLNADPRLHGILVQAPLP
                     SHLRASVVYASVLPEKDVDGFHPVNVGKLMLGDPTGFLPCTPAGIRELLARSEVQMSG
                     AEVVVLGRGNIVGKPMAAMLCQKAKHANATVTICHSATRDIRAHCRRADILIAAMGVA
                     EFVKADMVKPGAVVVDVGVNRVSDPAVKGGSRLVGDVDFAGVQPVAGKITPNPGGVGP
                     MTIAMLMQNTVRAAERAGGLEPKSEARNLKRV"
     gene            complement(2459..3682)
                     /locus_tag="VIL39_10270"
     CDS             complement(2459..3682)
                     /locus_tag="VIL39_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY5481855.1"
                     /translation="MKNNCWLILGMMLSTSLLAQQATNPTPAAPIEMPAAAPVAKIAP
                     ATTGTKAPAAKAGKKKSANKKAQKAPQKKAAKKAAAKKKEALPELRTVPLVAGPAVVV
                     ASNVNVRGQAKLKSEVVTRITKGQSVSVLEEIVRNNSGPDEPSAWAKISLPPGAHVWI
                     NTAFLNAANQTVRPKKLNLRSGPGENYSVIGLVHGGDAVKPMTTKGDWTEIEAPAGAY
                     AFVAAQYLKQGLPEAPVPIVAAAPTPAPAPTPTPAPAPIPVPPEPAPTPTAVAAAPTM
                     APPPTENPVVPAPTEPPAVTSAPTPPPTPAPAPIVTPTETPAEPAVAEPLPPRIVQRE
                     GVVRGMTSIQAPSKFVLISPENGRDIDYLYTTSKELDLRRYKGLRIIVTGEEALDERW
                     GNTPVITIQKILVVE"
     gene            complement(3686..4477)
                     /locus_tag="VIL39_10275"
     CDS             complement(3686..4477)
                     /locus_tag="VIL39_10275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5481856.1"
                     /translation="MRNAECGAGNQEHRTPSLLSIPQSAVRIQMVRLQKFLAEAGVAS
                     RRAGEQIIVAGRVEVNGKIVVELGTRVDPDHDRVAVDGKPVQTKRKLYVALNKPPRWV
                     CSRKDEFERPTIFELLPREWGHLHSVGRLDYNSEGLIFLTNDGQFSLHLTHPRYGVHK
                     KYVVTVEGRVAAEVLGQFTRGLVYQGDKLKAEKARLVSASQAQSVVELELAQGKYHEV
                     RRLFESQQLTVKRLQRVQIGKIKLGELPIGKWRTLTEPEIKSLMC"
     gene            complement(4482..4601)
                     /locus_tag="VIL39_10280"
     CDS             complement(4482..4601)
                     /locus_tag="VIL39_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HEY5481857.1"
                     /translation="MGGKRNVLKRFERLEVLKKRGQWKEGDRITGLRKTKKDA"
BASE COUNT          862 a         1612 c         1268 g          859 t
ORIGIN      
        1 caatggcgcc tttgaagtcc atggtgctca cgtggctttt gcccgccaaa aaatacggct
       61 ccttctcctc gtctcccggg gcagcgggca ggcacccgcc cagcacacag ccggcgagca
      121 gaaacagcgc gctgcgaaaa aggcccactg aaaacatcat ggttggagat taaacaaacc
      181 ggcagcgtca ggcaatcatg cgaacggatt ttcctcccct gtcgccaccg gctgccgcgg
      241 caagcacagg aggctgacga ggagataaat ccagcccatc accaaatgca cggcggcggc
      301 caccgccagc cgcacgatgt ccaacgcgcc ccagccgtac aggaagatcg cgccgcacag
      361 aaacagcgct cccggcatca acgcggcgcc tgccaggcgc caactcccgc acaagctcaa
      421 ctctcgatcc gcaaagaacc cgaccagcca cgccggcaga aaatacaccg tcgccaaaca
      481 cgcccaggtc accatcaggc tcaccaccac caaccctgct gcgatggcta gaagcggcgg
      541 cgcccaggcg ccccaccacg gcccgagttc gctctggttg aaggccacca cccagcttcg
      601 cggatacgcg tcttgcacgt atccgaacag agaaaagacc ttgcagtcgg tccggccaaa
      661 ctccacctgc acctgcgccg gcgaacgcgc ttccccggcg tggtccaaat cgacgacgag
      721 cgccagaaac tgcccctcag ccaggcgcga gggcgtcgaa ccttgccaat ccaatctgcc
      781 ggaacgaagc tctccttgcg gcggcagttg actgatcgcc tcgccgatgg tgggaaacca
      841 ggcgtgatgc aagaaccaca cgacgctggc ggcagccagg agtgcgacaa tgaattgcac
      901 cagcagcaat cgcctcaagg gtgcgcacgc aaaagcggcc atcccacgta gcgtcagcgg
      961 ttgccaggcg actggtgctt tctcgaccga ttcattcata cgaggcccaa cggagcgcca
     1021 accgccccgg cgaacggtct tcatgagccg cccgaggcca ccccggcaac cgctcagggc
     1081 gcgggcgttt gcgaagccgg tggtgccgat ggcaccggcg actggttggg tggtggtgtg
     1141 acgattacag gcactggata gcgcagtttc atcacgtcat ccaccagcac ttccacatag
     1201 tatgtgccgg ccaccttgaa ctcgacctgg ccgaacacgg tgacattggt tgcatgaagg
     1261 ccggcgtccc gcagaccgaa tttcacttcg cccttgcgca gaaccgtggt ctggtcgggc
     1321 gcaatgaacc gcgaagtctc aacaaactgg cccacgcccg cagtccagcg gttgaacaca
     1381 ctcagcttga atgccatcac cggcagttgc ggcacgcgaa tgaaacttgt cacgccgatc
     1441 aggaagaaat tcccgttggc ttcctggcgg acttcctcgc acatcagcga gcactgcaaa
     1501 tcaggcaaaa ttcgggtcgc ttgcatcgga tctcacactc gtttcagatt tcgggcttcg
     1561 gatttcggct ctaacccccc ggcccgttcc gccgcccgga cggtattctg catcagcatg
     1621 gcaatcgtca tcggccccac cccgcccggg ttgggcgtga tcttgccggc aaccggctgc
     1681 acgcctgcaa aatccacatc gccgaccaat cgcgagccgc ccttgaccgc gggatcactc
     1741 acccggttca cgcccacatc caccaccacc gcgccgggct tgaccatgtc cgctttcacg
     1801 aactccgcta cgcccatcgc cgcgatcaga atgtccgctc gccggcagtg cgcccggatg
     1861 tcgcgggtcg cggaatggca aatcgtcacc gtggcattgg cgtgcttcgc cttctggcag
     1921 agcatggccg ccatgggctt gccaacaatg ttgccgcgcc ccagcacgac cacttccgcg
     1981 ccgctcatct ggacctcgga ccgcgccagc aactcgcgta tgcccgccgg ggtgcaaggc
     2041 agaaatcccg tcggatcgcc cagcatcaac ttgcctacat tgaccggatg aaacccgtcc
     2101 acatccttct ccggaagcac gctggcatag accaccgagg ctcggagatg ggagggcagc
     2161 ggagcttgca ccagaatccc atgcaggcga gggtcggcat tcagccggtc caaaagaccc
     2221 agcagttcgc cttcactggt cgcttcgggc aacacgtgca tttccgaata aattcccagc
     2281 tcggcgcacg ttttctcctt ccgcccgaca tacaccttcg acgccgggtc ttcgcccacg
     2341 cgtacgaaag caaggcctgg cgacacacct ctcgccttca acgccgcgat gcgttgaacc
     2401 aactcgcctt gcagttgccg ggcgataccc cgcccatcaa taaggttcgc tggcggcatt
     2461 actccacgac cagaatcttc tgaatcgtaa tgaccggggt gttcccccag cgctcgtcca
     2521 gagcctcctc acccgtcacg ataatccgca gccctttgta acgtcgcaga tcgagctcct
     2581 tcgaggtcgt gtagaggtaa tcaatatccc ggccgttctc cgggctgata agcacaaatt
     2641 tcgatggcgc ctgaatacta gtcatgccgc gcacgacacc ttcacgttgc acaatccgag
     2701 gcggcagagg ttccgccacc gctggctcgg ccggcgtctc agttggcgtc acaatcggcg
     2761 ccggcgccgg agtcggcggc ggcgttggtg cgctcgtcac tgcgggcggc tccgtcggag
     2821 ctggcaccac tggattttcg gtcggcggcg gggccatcgt tggagccgcg gccacagcgg
     2881 tcggcgtcgg cgctggctcc ggaggcaccg ggatgggtgc gggagccgga gtgggagtag
     2941 gcgctggagc gggagtggga gcggccgcca caatgggtac gggagcctca ggcaatccct
     3001 gtttcagata ttgtgccgcc acgaacgcat acgcgcccgc cggtgcctcg atctcggtcc
     3061 agtcgccctt cgtcgtcatc ggcttcaccg cgtcgccacc atgcacgaga ccaatcacgc
     3121 tgtagttctc gcccgggccg ctgcgcaagt tcagtttctt gggccggacg gtttgattcg
     3181 ccgcgttgag gaatgcggta ttgatccaca cgtgcgcccc ggggggcagc gagatcttcg
     3241 cccaggccga cggctcgtcc ggcccggagt tgttccgcac gatctcctcg agcacggata
     3301 ccgactggcc cttcgtgatc cgcgtcacga cttcgctctt cagcttggcc tgcccgcgca
     3361 cgttgacgtt gctggccacg accaccgccg gcccggccac caacggcacg gtcctcaact
     3421 ccggcaaagc ttccttcttc tttgccgcgg ccttcttggc ggccttcttc tgtggcgcct
     3481 tttgagcctt cttgtttgcg gacttcttct tccccgcttt agccgccggc gcttttgtcc
     3541 cggtggtcgc gggcgcgatc tttgccaccg gcgcggccgc cggcatttca atcggcgccg
     3601 cgggcgtcgg attggtcgct tgttgcgcca acaagcttgt tgaaagcatc atccccagaa
     3661 ttaaccaaca gttgtttttc ataactcagc acattaaaga tttaatctcg ggttctgtca
     3721 atgtccgcca tttgcctatc ggcagctcgc ccagcttgat tttcccgatc tggacccgct
     3781 gcaaccgctt taccgtcaat tgctgcgact cgaacagccg ccgcacttca tgatacttgc
     3841 cctgcgccaa ctccagttcc accacgctct gcgcctggct cgccgaaacc agccgcgcct
     3901 tttccgcctt caacttgtcc ccctgataca ccaggccgcg cgtgaactgg cccagcacct
     3961 ctgcggccac ccggccctcc accgtcacca catacttctt atgcaccccg tagcgcggat
     4021 gcgtcaagtg caggctgaac tggccgtcgt tggtcaagaa gatcaaccct tcgctattat
     4081 aatccagccg ccccaccgaa tgcaggtggc cccattccct gggcagcaac tcgaaaatcg
     4141 taggccgctc aaattcgtcc ttgcgtgagc acacccaacg cggcggcttg ttgagcgcca
     4201 cgtaaagttt gcgcttcgtc tggaccggct tgccgtccac cgctacccgg tcatggtcgg
     4261 ggtcaacccg cgtccccaac tccaccacga tcttgccgtt gacctccacc cgcccggcca
     4321 caatgatctg ctcgccggcg cgccgcgagg cgacacccgc ttccgccagg aatttctgca
     4381 agcgaaccat ctgaatgcgg actgcggact gcggaatgga cagcagcgac ggcgtgcggt
     4441 gctcctgatt ccccgctccg cactccgcat tccgcattgg cttacgcgtc cttcttggtt
     4501 ttgcgcaagc cggtgatgcg gtctccctct ttccattgcc cgcgcttctt gagcacttcg
     4561 aggcgctcaa agcgcttgag gacatttcgc ttgccgccca a
//
LOCUS       DATCGS010000502         7022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_538767, whole genome shotgun sequence.
ACCESSION   DATCGS010000502 DATCGS010000000
VERSION     DATCGS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538767"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VIL39_10285"
     CDS             complement(<1..1175)
                     /locus_tag="VIL39_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY5481858.1"
                     /translation="MLRLLRDRFQPTLALKLGFLVLVSTGMIFFAAFGYYNHVVRGLM
                     LKSVEENARLLARGTVNRIESVLHAVEPLPESLGLQIANERHTEGEITRLIETELRVN
                     PHIYGSAVAFAPGVSAPGTGSAAPYVYRKGDRFQFADLAQDDYSYQTQDWYLIPRETG
                     KAMWTDPYFDEGGGEAKMCTYCVPFYREGPGQKVFAGVVTADVLLDDMVRTVSSIKLY
                     ETGFAVLLSQSGQFISHPRTNYVFKESIFSVAAEANSAIWREIGRKMVRGEEGFLRTR
                     SLSTGQIVWMYYAPISNGWSVGMLLPESEVLRDLRAMNRVVLWIGLIGFGLLLAVITA
                     LSLGVTRPIRALAKQTQAIAQGNLNLAVPPSTSRDEVGMLSRSFEDMRLALREYVANL
                     "
     gene            complement(1156..2034)
                     /locus_tag="VIL39_10290"
     CDS             complement(1156..2034)
                     /locus_tag="VIL39_10290"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5481859.1"
                     /translation="MPHWIPQAQFAGYYMALERGFYRQHGLDVQILDGGPRKSVGKLL
                     VSGEATFAGHFLSSALKLRDEGIPVVHLAQITQRSALMLVAYKSHGIRSVQDLEGKKV
                     SVWPAFSAQPQALFRKHNLHVQTVPQGQTIGLFMRGGVDAASAMWYNEYHLILNSGLN
                     EDELTLIFYDQNDLNFPEDCILCLAETWRTRPELCREFVQATLEGWQYVLSHREEALN
                     VVMQRTEQARTGTNRNHQRWMLGCMCELIRPVKPGTALGELSSDDYERMARELKQNGE
                     IKTAPAFKEFHAPPPS"
     gene            2297..2722
                     /locus_tag="VIL39_10295"
     CDS             2297..2722
                     /locus_tag="VIL39_10295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5481860.1"
                     /translation="MSTRPGNIFERRFHNQLDDLSRVTQEAVQFIEENGVDSQAVYLA
                     NLAIEELATNILKYGYDDTAVHEILLRLEIHPGSLLLVLEDDGHEFNPLAAPEPEVNL
                     PTEQRVPGGMGIHLVRKLAEHVDYERCRGLNRITVRIRS"
     gene            2746..3552
                     /locus_tag="VIL39_10300"
     CDS             2746..3552
                     /locus_tag="VIL39_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY5481861.1"
                     /translation="MQKYWHVINIGIQNNLTYRFNFLARAVFGLIPLIAMLYMWQKIY
                     SGNGAGTTVGTYSLAQMISYYLLTTVVDALTAVNEDDWQIAADIKDGNISQFLLKPID
                     YLWYRLCLFLSGRVTYLAVAVTPLALFILYLHQYFVLPPDWTAFACFLLSTVLTALLQ
                     FFMSYAMAMLAFWVLEVSTFIFILFAFEFIASGHLFPLDILPHGLEQALYFTPFPYQL
                     YFPVSIYMGKTTGANLMSGLLIQALWVIAAYCIARFAWRRGIKKYSAVGG"
     gene            3557..4447
                     /locus_tag="VIL39_10305"
     CDS             3557..4447
                     /locus_tag="VIL39_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY5481862.1"
                     /translation="MKSKVQSPKPKVEPSGPPHATRPPPFPSRITHHVSRYLSIYAAL
                     WKNSVAREMSFKSNFLLWILVEFLWFGLQLSFIGVLYLHTDHIGTWTKWQVVMLIGAS
                     HFIQQLFQAFFLINCTNLSELVRSGKLDFLLLLPVNTRFVVSLRQVDLGAFVNAASAV
                     AVMAYAAHQLHLAPTLVQVLGFLVLSAAGIAIHYSLMFLLASISFWTVRAQGIVWGYY
                     NLFNIARLPDEAFSGLFKAVFTFAVPMLLVSNVPARLLADKLNSPSQVFLLLTMTVVC
                     FCVSEWGWRASMRRYTSASS"
     gene            complement(4593..>7022)
                     /locus_tag="VIL39_10310"
     CDS             complement(4593..>7022)
                     /locus_tag="VIL39_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY5481863.1"
                     /translation="AQYRDATLGLLVTVENAGPQPATVSLEGTLLDTTGKSVAIPRME
                     MRVDPGASGGQAKISASIVNPLKWSAETPNLYKLLLTLKDARGTVLEVIPANVGFRQV
                     EIRDGNLLVNGRRILIKGVNRHEFEPDRGQAITVASMEKDIQVMKQFNINAVRCCHYP
                     NQTAWYDLCDRYGIYLIDEADIESHGMGYGPETLAKNPDWADAHMNRTVRMVERDKNH
                     PSVIIWSLGNEAGDGPNFEATSKWIHQRDPSRPVHYEQAGHKPHTDIVCPMYPPPREL
                     DKYAAKAQTRPYIMCEYEHAMGNGSGDFWSYWGQIYSKPYLQGGFIWDWVDQGLRQRQ
                     QPLPLAHFEKVKPGDKTFWAYGGDFGPAGTPSDDNFCCNGLVTPDREPHPGLHQVKHV
                     YQYIHCQAGDLANRTVEVKNWYDFTNLKDLAAGQWRLKADGKVIQSGRLPVLEVPAGA
                     STRLTIPTKAFNPLPGIEYFLELTFTLQHDQPWAKAGHEIAWDEFKLPDFAPPLAAPA
                     DKSAAPKLAQSGATITVSGQNFEVVFDKQAGALTSWRFRGDQLVKSPLRPDFWRAQID
                     NDRGRNMLRSQGVWRDAHEGAEVRSVTVQEQPQSRAVGVKVITALPKVDAAWETDYTI
                     CGNGDVVVEAHFKPNKTDLPKLVRLGMQMALPAGFERITWLGPGPQETYCDRKDARFG
                     LYTGTVADQFYADYTKPGESGNKVDLRWLALANGKIGLLAIPDRPGLLSANALHYATA
                     DLNAGKHAFELPRRDFVTLNLDLKQQGVGGDNSWGAWPHEEFLIPCQEYTYSFRLRPF
                     VAGQDVAKLAR"
BASE COUNT         1310 a         2192 c         2076 g         1444 t
ORIGIN      
        1 aggttggcca cgtattcccg caaggccagc cgcatatctt caaacgaccg cgagagcatg
       61 ccgacctcgt cgcgggacgt ggacggcggc accgccaggt tcagattacc ttgcgcgatt
      121 gcctgcgtct gtttggccag cgcgcggatg ggccgcgtga cacccagcga gagcgccgtg
      181 atcaccgcca acaacaaccc gaagccgatc agcccaatcc aaagcacaac ccggttcatg
      241 gcgcggaggt cgcgcaacac ctctgactcc ggaagcagca tccccaccga ccaaccgtta
      301 gagatgggcg cgtagtacat ccacacgatc tggcccgtgc ttagggaacg ggtgcgcaga
      361 aaaccctcct cgccgcgtac catcttccgc ccaatctccc gccagatggc actattcgcc
      421 tccgcggcca cactgaaaat gctctccttg aaaacgtagt ttgttcgggg atgggagatg
      481 aactggccgc tttgggagag cagcaccgcg aagccggtct cgtaaagctt gatcgaagag
      541 acggtacgga ccatgtcgtc cagcagcacg tcggcggtga ccacgccggc gaagaccttc
      601 tgccccggcc cctcccggta aaacggcacg cagtaggtgc acatcttcgc ctcgccccca
      661 ccctcatcga agtagggatc ggtccacatc gctttgccgg tttcgcgcgg gatcaggtac
      721 cagtcctggg tctggtagct gtaatcgtcc tgggccaagt cggcgaactg gaaccggtcg
      781 cccttgcggt acacgtacgg agccgcagat ccagttcctg gggcagaaac gccgggcgca
      841 aacgccacgg cgctgccgta aatgtgcggg ttaacacgca gttcggtctc gatcaggcgc
      901 gtgatttccc cctccgtgtg gcgttcgttt gcaatctgga gtccgagcga ttccggcagc
      961 ggctccaccg cgtgcagcac ggactcaatc cggttgacag tgccgcgggc gaggagccgt
     1021 gcgttctcct ccaccgactt cagcatcagc ccacgaacca catggttgta atacccgaag
     1081 gcggcaaaga agatcatccc ggtactgacc agcaccagga accccagctt caacgccagc
     1141 gtgggctgga accggtcacg aaggaggcgg agcatggaat tccttaaaag caggcgcggt
     1201 cttgatctcg ccgttttgct tcaactcgcg cgccatacgt tcgtagtcgt cactcgacaa
     1261 ttcgcccagg gcggtgccag gcttgaccgg acggatcaac tcgcacatgc accccagcat
     1321 ccaacgctga tggttgcggt tcgtgccggt gcgggcctgt tcagtgcgtt gcatcaccac
     1381 attcagggcc tcctcgcgat gactgagcac gtactgccag ccttccagcg tggcctgcac
     1441 aaactcgcgg caaagctcgg gccgggtccg ccaggtttcg gctaggcaga ggatacagtc
     1501 ttcggggaag ttgaggtcgt tctggtcgta gaagatgagc gtcaactcat cctcgttcag
     1561 accggagtta agaatgagat gatactcgtt gtaccacatc gccgaggcag catccacgcc
     1621 cccgcgcatg aacaggccga tcgtctgtcc ttgcgggaca gtttgcacgt ggaggttgtg
     1681 cttgcgaaac aaagcctgcg gctgcgcgga gaaggcgggc cacacgctca ctttcttgcc
     1741 ctcgagatcc tgcaccgagc gaatcccgtg agacttgtaa gcgacgagca taagcgcgga
     1801 gcgctgggtg atttgcgcga ggtggaccac gggaatccct tcgtcgcgca gtttcaacgc
     1861 ggaagaaagg aaatgaccgg cgaaggttgc ctcgccggaa accaacaact tgcccacgga
     1921 cttgcgcggg cctccgtcga ggatctgcac gtccaaacca tgctgccggt agaaaccccg
     1981 ttccaacgcc atataatagc ccgcgaattg ggcctgggga atccagtgtg gcaagaacac
     2041 aaccctggtc agcgttctgt ctccctcggc tgctgcggcg cacagcggaa gcaagaatgc
     2101 gatggccagc gcccagagca agcctccagg cttagttttt ttcggttgtc gagccgtcgc
     2161 agggtacata caatgattac accggtttta gcgatattgg catggcgatg cggatgcctg
     2221 caagttggat cggcttattt ccccagccgc ctccaggctc gccatttgaa tcggggaagc
     2281 agaatctctt gttttgatga gcaccaggcc gggcaacatt tttgaacgcc gtttccataa
     2341 tcagctcgac gacctgagcc gcgtcacgca agaggcggtg cagttcattg aagaaaacgg
     2401 cgttgatagc caggcggttt atctggccaa cctggccatt gaagaactgg ctaccaacat
     2461 cctcaaatac ggttacgacg acacagcggt ccacgagatc ctattgcgac tggagatcca
     2521 tccaggttcg ctcctgctgg tgctcgaaga cgatgggcac gaattcaacc cccttgcggc
     2581 gccggagccg gaggtcaacc tgccgaccga acagcgggtt cccggaggca tgggaatcca
     2641 tctggtccgc aaattggccg aacatgtaga ttacgagcgc tgccgcgggc tgaatcggat
     2701 cacggttaga atccggtcct gacgctataa accacagaca gcacgatgca gaaatactgg
     2761 catgtcataa acatcgggat tcagaacaac ctgacatacc ggttcaactt cctggcgcgg
     2821 gcggtgttcg gcctcattcc gctcattgcc atgctctaca tgtggcagaa aatctactcc
     2881 ggcaatggcg cggggacgac ggtcggcacc tattcgctgg cgcagatgat ctcgtattac
     2941 ctgctcacca ccgtcgtgga cgccctaacg gcggtgaacg aggacgattg gcagatcgcc
     3001 gccgacatca aggacgggaa catcagccag ttcctgctca aaccgattga ttacctctgg
     3061 taccggctct gcctgttcct gtccggccgg gtgacctacc tcgccgtggc tgtgaccccg
     3121 ctagcgctct tcattcttta cctccaccag tattttgtgc tgccgccgga ttggactgcg
     3181 ttcgcttgct ttctcctctc gacggtcctg accgcgctgc ttcagttctt catgtcgtac
     3241 gcgatggcga tgctggcgtt ctgggtgctc gaagtctcga cctttatctt catcctgttc
     3301 gccttcgaat tcatcgccag cggccacttg ttcccgctgg acatcctgcc gcacggcctc
     3361 gagcaggctc tctacttcac gccgttcccc taccagctct attttcccgt gagcatttac
     3421 atgggcaaga cgacgggcgc gaacctgatg agcggtttgc tgatccaggc tctatgggtc
     3481 atcgcggctt attgcatcgc gcgattcgcc tggcggcggg gcatcaagaa gtattcggcg
     3541 gtaggcggat gatgacatga agtccaaagt ccaaagtcca aagcccaaag tcgagcccag
     3601 cggcccgccg cacgcaacac gccccccgcc cttcccttca cgcatcacgc atcacgtatc
     3661 acggtatttg tccatctacg ccgccctgtg gaagaactcg gtcgcgcgcg agatgagctt
     3721 caagagcaac ttcctgctct ggattctagt ggagttccta tggttcggcc tgcagttgag
     3781 cttcatcggc gtgctctacc tgcacaccga ccacatcggc acgtggacca agtggcaggt
     3841 ggtcatgctg attggcgcca gccacttcat tcagcagctc ttccaggcct tctttctcat
     3901 caattgcacc aacctttccg agctggtgcg cagtggcaaa ctggacttcc tgctcttgct
     3961 gccggtcaac acgcgcttcg tcgtgtcgct gcgtcaagtg gatctcggcg ccttcgtcaa
     4021 cgccgcctcg gcggtcgccg tgatggccta tgccgcgcac cagttgcacc tggccccgac
     4081 gctggttcag gtgctgggtt tcctggtgct cagcgcggcg ggcatagcga ttcactactc
     4141 gctgatgttt ctgctggcca gcatcagctt ctggaccgtg cgcgcgcagg ggattgtgtg
     4201 gggctattac aacctgttta acatcgcccg cttgcctgac gaagcgttca gcgggctgtt
     4261 taaggccgtg ttcacgtttg ccgtgccgat gctgctggtt tccaacgtgc ccgcgcggtt
     4321 gctggccgac aaactgaact ccccctccca ggtgttcctg ctgctgacca tgaccgtcgt
     4381 gtgcttttgc gtctccgaat ggggctggcg cgcctctatg cgccgctata ccagcgccag
     4441 ttcctaaacc cgctccttaa cgcaaacgcg ccaagctgca aaggcgcaac cgaaccgcgc
     4501 aagaaccact tccggcctgc gtccgcgtcg gtctgagcac gaccacgcca gaccggtcaa
     4561 ctaccttccg aaatcgctgt cccggcacac actcacctcg ccagcttggc aacgtcttgc
     4621 ccggcgacaa acggccgcag gcgaaagctg taagtgtatt cctggcaggg gatgaggaat
     4681 tcctcgtgcg gccaggcgcc ccaactgttg tcgccgccga caccctgttg cttaaggtcg
     4741 aggttcaggg tgacgaagtc gcggcgtggc aactcaaaag catgcttgcc ggcgttcagg
     4801 tccgcggtgg cgtaatgcag cgcgttggcg ctgagcaatc ccggtcggtc gggaattgcc
     4861 agcagaccga tcttgccgtt cgccagggcg agccagcgca agtccacctt gttgccgctc
     4921 tcgccgggct tggtgtaatc agcataaaac tggtccgcaa ccgtgccggt atagaggcca
     4981 aaccttgcgt ccttgcggtc gcagtacgtc tcctgcgggc cgggacccag ccaggtaatg
     5041 cgctcgaaac cagccggcag cgccatctgc atgccgagac gaacgagctt gggcaaatcg
     5101 gtcttgtttg gcttgaagtg ggcctcgaca acgacgtcgc cgttgccaca tatcgtgtag
     5161 tccgtctccc acgccgcgtc caccttcggc agcgctgtaa tcaccttgac ccccacggca
     5221 cgcgactggg gctgctcctg gacggtaacc gagcggactt ccgcgccctc gtgcgcgtcg
     5281 cgccagacac cttgagacct gagcatgttg cgcccgcggt cattgtcaat ttgggcgcgc
     5341 cagaaatcgg gccgcagcgg actcttgacc aactggtctc cccgaaagcg ccaggaggtg
     5401 agggcgccgg cctgtttgtc gaagacgacc tcgaagttct gtccgctgac ggtaatggtc
     5461 gccccactct gcgccagctt gggcgccgcc gatttgtccg ccggcgcagc cagaggcggc
     5521 gcgaagtccg gcagcttgaa ttcatcccag gcgatttcat ggcccgcctt ggcccaaggc
     5581 tggtcgtgct ggagagtgaa ggtcagttcg aggaagtact ctatgcccgg caggggattg
     5641 aacgctttgg tcggaatggt gaggcgtgtg ctggcgcccg cgggtacttc caacacgggc
     5701 aggcgcccgc tctggatcac tttgccgtcc gccttgagcc gccactgccc ggcggcaagg
     5761 tctttcaggt tggtaaagtc ataccagttc ttcacctcga ccgtgcggtt ggcgaggtcc
     5821 ccggcctggc agtggatgta ttgatagacg tgtttgacct ggtgcaggcc aggatgcggt
     5881 tcgcgatcgg gcgtgaccag gccgttgcag caaaagttgt catcgctcgg cgtgccggcc
     5941 gggccgaagt cgccgccgta ggcccagaag gtcttgtcgc cgggcttgac cttctcaaag
     6001 tgcgccagcg gaagcggctg ctgtcgttgc cgcaaaccct ggtccaccca atcccaaatg
     6061 aagccgcctt ggagataggg tttgctgtag atctgccccc aatacgacca gaagtcgccg
     6121 gaaccgttgc ccatggcgtg ctcgtactcg cacatgatgt aaggacgcgt ctgcgccttg
     6181 gcggcgtatt tgtccagctc gcgcggaggc gggtacatcg ggcagacgat atccgtgtgc
     6241 ggcttgtgcc cggcctgttc gtagtggaca gggcggctgg ggtcgcgctg gtgaatccac
     6301 ttcgaggtgg cctcgaaatt cggcccgtcg cccgcttcgt tgccgagcga ccagatgatg
     6361 acggacggat ggttcttgtc gcgctcgacc atgcgaacgg tgcgattcat gtgggcatcg
     6421 gcccaatccg ggttcttggc cagcgtttcg ggaccgtaac ccatgccgtg gctttcgata
     6481 tcggcctcgt caatgaggta aatgccgtag cggtcgcaga gatcgtacca ggcggtctgg
     6541 ttcgggtaat ggcagcagcg cacggcgttg atgttgaatt gcttcatcac ctggatgtcc
     6601 ttctccatgc tcgccaccgt gatggcctgc ccgcggtccg gctcgaactc atggcggttg
     6661 acgcctttga tcaggatgcg ccggccgttg accagcaggt tgccgtcgcg aatctcgacc
     6721 tggcggaagc cgacgttcgc gggaatcact tcgaggaccg tgccccgcgc gtctttgagc
     6781 gtgagcaaca gcttgtagag gttgggtgtt tccgccgacc acttgagcgg gttgacgatg
     6841 cttgccgaaa tcttcgcctg cccaccgctg gcacccgggt ccactcgcat ctccatgcgc
     6901 ggtatagcga cacttttgcc ggtcgtgtcc agcagtgtgc cctcaaggct gaccgtcgcc
     6961 ggttgcggac ccgcgttttc gacggttacc aacaagccga gcgtggcatc gcgatactgc
     7021 gc
//
LOCUS       DATCGS010000503         5172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_489255, whole genome shotgun sequence.
ACCESSION   DATCGS010000503 DATCGS010000000
VERSION     DATCGS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489255"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1404
                     /locus_tag="VIL39_10315"
     CDS             397..1404
                     /locus_tag="VIL39_10315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5481864.1"
                     /translation="MSTSDKPLKMVTCSGCEAKVFIPGDLAPLSTEPCKRCGHAIMMP
                     MQLRQFELRSKIASGGQGKVYRAFDTVLERLVAVKLMRKEIENDPLALAAFYREARAC
                     ASLNHTNIIHIYSFDEFEGQRYLVMELADRGSLDQRIEKQTCLRELDVLDIGYKIASA
                     LDMALKHNLLHRDIKPGNILFNADGEPKLIDFGLARSAEAEVESSSVTEGTPYYVAPE
                     KIKRERETFLSDMYSLGCTLYHALTGHVPFEAPSVEELVAAHVNTPLTPPILVLPEIT
                     QPTNDVLVKVLAKNPADRYLSYDEFCAALEMARSHLLVQQQIQNTQGKDVKSKTSWWR
                     R"
     gene            complement(1445..1858)
                     /locus_tag="VIL39_10320"
     CDS             complement(1445..1858)
                     /locus_tag="VIL39_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481865.1"
                     /translation="MMKSVLKIGALGLLAFAIASTPAQLLAQSTNKANAAKQPSVAKT
                     EAATKKKSAHPFNGKLAAVDKTAKTIKVGQSVYLVTSATKITKDGKPATLEDGVLGEP
                     VSGYVKPTEDGKMAATTLRFGAKVEAQGGSKKKEK"
     gene            complement(1949..2461)
                     /gene="lptE"
                     /locus_tag="VIL39_10325"
     CDS             complement(1949..2461)
                     /gene="lptE"
                     /locus_tag="VIL39_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY5481866.1"
                     /translation="MRYWQWLLLGLVALGGNGCAGYRLGPVNGVVAREKSVQVNPFSS
                     RTLEPRLSDAVTSQLRKQLQRDGTYQLATRDDGDIVVSGIVTRYDRHEISFSSSDILT
                     VLDYRLDLEAQVTARERSTGKIILNQPVTGSTLIRVTTDLTSSERQALPLLAADLAKN
                     VTALLTEGAW"
     gene            complement(2463..3470)
                     /gene="bamD"
                     /locus_tag="VIL39_10330"
     CDS             complement(2463..3470)
                     /gene="bamD"
                     /locus_tag="VIL39_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY5481867.1"
                     /translation="MHYRFIRLVLIAACLLAFPFRAPAPLVYRPGEGWTYEPVGSGGR
                     WQRPRAKEQLEVAQAAFDRKAYGLALKAARHVLVRWPESDYAPQAQNLVARSYEAKGD
                     AEKAFKEYQKLLEKQPKTASFDEIRRRQYEIANLFLAGKRFKLWGTIPLYRSMDRTAK
                     MYKDIVKTGPYSDVAPQAQLNIGAAREKQSSLGFKAPDYTAAIQAYDLAADRYHDQPK
                     VASEAIYRAGLAYQKQAQSPDRDQGAAGQAIAKFTDFLALYPDDPRAPQAHQIIASLK
                     SKQAQGNFVVAQFYEKRKRWEGARIYYNEVLLQDPNSPYAPEARKRIDQLKQRTQGAG
                     K"
     gene            complement(3584..4102)
                     /locus_tag="VIL39_10335"
     CDS             complement(3584..4102)
                     /locus_tag="VIL39_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481868.1"
                     /translation="MDVIFNCPKCEQELAVDSTGIGSELNCPSCGEAIVIPKPESVVN
                     RPGVNQAPDAPRTEVHPVNAISSSAAAKVEMHLRVPEHNTPTESLIVKPLVPLEAAAK
                     TTGKVIRIRTIKHTDCIEVGHDKFDEVVSNFLAKIGESNMISLSTIVYTHLDIGTQKL
                     LQDFGVLVVYRG"
     gene            complement(4171..4575)
                     /locus_tag="VIL39_10340"
     CDS             complement(4171..4575)
                     /locus_tag="VIL39_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481869.1"
                     /translation="MTKLIAALLIVAALFGAWELFLYWEKVKNDEETNQKKEAAAMVL
                     GDQLPGMSPTLDASLAAARKQGAAGLRNWLKTHNQSVEDPRKAWIELDYCMAVARENT
                     SEAKRVFAAVKERTPPSSPVWPRIKQLEKTYE"
     gene            complement(4572..>5172)
                     /locus_tag="VIL39_10345"
     CDS             complement(4572..>5172)
                     /locus_tag="VIL39_10345"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034784.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5481870.1"
                     /translation="ELHGAEHEVIPDRIEAATYAIAAVATNGEIKLRGARADHMRAVL
                     DKLKDAGAGIERCGADITVRRNGRLKPVDITTLPYSGFPTDVQAQMMALMTLSPGTSI
                     ITERIFESRFMHVSELTRLGADIEIEGPSAIVKGGKPLSGAPVMASDLRASAALVIAG
                     LAARGTTQVNRVYHIDRGYENIDGKLRQLGARIERIEES"
BASE COUNT          926 a         1608 c         1568 g         1070 t
ORIGIN      
        1 ccgacatcac caccctctac acggcccgct acggcgtgcc gccggtcggc aagaaggtgt
       61 acgtccgggt gaaccagttc gttgacgggt ggtaagccct gcccgtgacc ttctcggcca
      121 tcgtcccggc ctcggcctaa ccttccgccg gggcgtgctg gccgtcgagc catggctcgc
      181 cctcggcttc cacgcgggca ggcgtcttcc cgggcgcctg cccgctagaa gacacgggcc
      241 acacggcgcg gttttactat cttgaaggcg tgacggttga cagcgcgggc aatgtgttcg
      301 tggcggacgc aggcaccgtg ccaaaaacca cttgagtgtt tggttttcga gcatgagact
      361 tgtagtggga acgaacgagg gtattattgc aatgctatga gcacgagtga taagccgctg
      421 aaaatggtca cctgcagcgg gtgtgaagcc aaggtcttca tccccgggga tctggcgccg
      481 ctgtccaccg agccgtgcaa gcgatgtggc cacgcgatca tgatgccgat gcagttgcgc
      541 cagtttgaat tgcgcagcaa gatcgcctcg ggtgggcagg ggaaggttta tcgcgctttt
      601 gacacggtgc tcgagcgcct ggtggcggtg aagctgatgc ggaaagagat cgagaacgac
      661 ccgttggctt tggcggcttt ctaccgtgaa gcgcgcgcct gcgcctcgct caatcacacc
      721 aacatcatcc atatctattc gttcgacgag ttcgaaggcc agcgctacct ggtgatggag
      781 ctggcggacc ggggcagtct ggaccaacgc atcgagaaac agacctgcct gcgcgaactc
      841 gacgtgcttg atatcggcta caagatcgct tccgccctcg acatggcgct caagcacaac
      901 ctgctccacc gcgatatcaa gccgggcaat attctcttca atgccgacgg cgagcccaag
      961 ctgattgatt tcggcctggc gcgcagcgcc gaggctgagg tcgagtcatc gtccgttacg
     1021 gagggaaccc cttactatgt cgcgccggag aaaatcaagc gggagcgcga gaccttcctg
     1081 tccgacatgt acagcctggg ctgcactcta taccacgcgt tgaccggcca cgtgccgttc
     1141 gaggccccga gcgtggagga gctggtcgcc gcgcacgtga acacccccct cacgccgccg
     1201 attctggtct tgcctgaaat cacccagccc accaacgatg tattggtgaa ggtgctggcc
     1261 aagaatccgg ccgatcggta tctgagctat gacgaattct gcgcggctct ggagatggcg
     1321 cgcagccacc tgctggtcca gcaacaaatc cagaacacgc agggcaaaga cgtcaagagc
     1381 aagacaagct ggtggcggcg ctgaggcgcc gcgaagtggc cccaggctcc ggcggtggag
     1441 cggtttactt ctctttcttc ttcgagccgc cctgggcttc aaccttggcc ccaaaacgca
     1501 gggtcgtggc ggccattttc ccgtcctcgg tcggcttgac ataaccgctg acgggctctc
     1561 cgaggacgcc gtcttccagg gtggctggct tgccgtcttt ggtaatcttc gttgcggaag
     1621 tgaccaggta aaccgactgt cccaccttga tggtcttggc ggtcttgtcc accgctgcca
     1681 gttttccatt gaagggatgc gccgatttct tcttcgtggc tgcttctgtc ttggccacgg
     1741 agggctgctt tgcggcgttg gctttattgg tgctctgagc caggagctgc gctggcgtgc
     1801 tggcaatggc aaaggccaag aggcctaagg ccccgatttt aaggacggat ttcatcatag
     1861 tcgtttaagt tcttggtggt cagctaagac ggcccattac taccaaattg ccgcgggcag
     1921 gcaagcccga cttatttaca gacccgagct accaagctcc ctcggtcaat aaggccgtca
     1981 cgttcttcgc caagtcagcc gccagcagcg gcagcgcctg ccgctccgag ctcgtcaaat
     2041 cggtcgtcac gcggataagg gtggaacccg tgaccggttg gttgagaatt atcttgccgg
     2101 tgctgcgttc gcgagcagtt acctgcgctt ccaagtccag gcggtagtcg agaaccgtca
     2161 ggatgtcgct cgaggagaag ctgatctcgt gccgatcgta gcgggtaact ataccgctaa
     2221 ccacgatgtc cccgtcatcc cgggtggcga gttggtaggt gccatcgcgc tgtaactgct
     2281 tgcgcagttg ggaggtgacc gcgtcgctca gacgcggctc cagagttcgg ctgctaaacg
     2341 ggttgacctg caccgatttc tcgcgcgcga ccacgccgtt gacgggcccg agccggtagc
     2401 ccgcgcagcc gttcccgcca agcgccacca gaccgagcag cagccactgc caatatctca
     2461 tgctatttgc ccgccccctg ggtccgttgc ttgagctggt caatgcgttt ccgggcttcg
     2521 ggcgcatagg gcgaattggg atcttgcaac aggacctcat tgtaataaat ccgcgcgcct
     2581 tcccaccgtt tgcgtttctc gtagaactgg gcgacgacga agttgccctg cgcttgtttg
     2641 cttttgagcg aggcgatgat ctggtgtgcc tgcggcgccc gcgggtcgtc cgggtagagc
     2701 gccaggaagt cggtgaactt ggcgatggcc tgtccggccg cgccttgatc gcggtccggg
     2761 ctttgcgcct gtttctgata agccaggccg gcccgataga tcgcctcgga ggccaccttt
     2821 ggctggtcgt gataacgatc ggcggcgaga tcgtaagcct ggatggcggc cgtatagtcg
     2881 ggggctttaa agccgagcga cgactgcttt tcccgcgctg cgccgatgtt caattgcgcc
     2941 tggggggcga cgtcgctgta cggccccgtc ttcacgatgt ccttgtacat tttggcggtg
     3001 cgatccatgg accgatagag gggaatggtg ccccacagct taaaccgttt accggcgaga
     3061 aacagattgg caatctcgta ctggcggcgc cgaatctcgt cgaagctggc agtcttcggc
     3121 tgcttctcca ggagcttttg gtactccttg aaggcctttt ccgcgtcgcc cttggcctcg
     3181 taactgcggg cgacgaggtt ttgcgcctga ggggcgtaat ccgattccgg ccacctcaca
     3241 agcacatggc gcgctgcctt gagcgcaagg ccataggctt tcctgtcgaa ggcagcctgg
     3301 gcaacttcca gttgctcctt ggcccgcggt cgctgccagc ggcccccgct gccgactggc
     3361 tcgtaggtcc agccttctcc cggccgatag accagcggcg ccggcgcacg aaacgggaag
     3421 gccaacagac acgccgcaat cagcaccagg cgaataaacc ggtaatgcat gaggacagga
     3481 taatgagaga cgagaggcta gtcaaggcga accgccagcc caaccgcaag gcccgccggc
     3541 ccatgccggg gtgctgctct gcgaggaccc gcagagttcc ggactagcct cgatagacga
     3601 ccagcacacc gaagtcctgg agcagcttct gcgtgccgat gtccaggtgc gtatagacaa
     3661 tggtcgagag gctgatcatg ttggactcgc cgatcttagc caggaagttc gacaccacct
     3721 cgtcaaactt gtcgtgaccc acctcgatgc agtcggtgtg cttgatcgtc cggatgcgaa
     3781 ttactttgcc cgtggtcttt gcggcggctt cgagcggaac gagcggcttc acgatcagac
     3841 tctccgtcgg cgtattgtgt tccggcacgc gcagatgcat ttccactttc gcggcggcgg
     3901 aagaggagat ggcgttgacg ggatggacct cagtgcgcgg cgcgtcggga gcttggttca
     3961 cgccgggacg gttgaccact gactccggct tcgggatgac aatggcctcg ccacacgatg
     4021 ggcagttgag ttccgagccg atccccgtgc tatccacggc cagctcctgc tcgcacttcg
     4081 gacaattaaa tattacatcc ataggcgtcc ttttgttttc cgactgcgcc gcgataataa
     4141 cacgcgctcc cgcccctgac cagcaaaaag ttactcgtaa gtcttctcga gctgtttgat
     4201 ccgcggccag acaggcgagg aaggcggcgt gcgctccttc accgcggcaa agacgcgctt
     4261 ggcttccgac gtgttctccc gggcgacggc catgcagtaa tccaactcga tccaggcctt
     4321 gcgcgggtcc tcgacgctct ggttgtgagt cttcaaccag tttctcagcc cggcggcacc
     4381 ttgcttccgc gcggccgcca gcgacgcatc gagcgtgggc gacatgcccg gcagttggtc
     4441 ccctaacacc atcgccgccg cttccttctt ttggttggtt tcttcgtcat tcttcacttt
     4501 ctcccagtac agaaacagtt cccacgcgcc gaagagcgcc gccacgataa gcagggctgc
     4561 aattagttta gtcatgattc ttcaatccgc tcgatgcgcg cgccgagttg ccgcagcttg
     4621 ccatcaatgt tctcgtaacc gcggtcaatg tgatagacgc gattgacctg cgtggtgccg
     4681 cgcgcggcca gcccggcgat caccagcgcc gccgacgccc gcaaatcact ggccatgacc
     4741 ggagccccgc tcaacggctt gccccccttc acaatcgcac tcgggccttc gatttcaatg
     4801 tccgccccca gccgcgtcag ttcgctgacg tgcatgaagc gcgactcaaa gattcgctcg
     4861 gtgataatgc tggtgcccgg cgacaacgtc atcaaagcca tcatttgcgc ctgcacatcg
     4921 gtggggaagc ccgagtaggg cagcgtcgtg atgtccaccg gcttcaaccg gccgttgcgg
     4981 cggacggtga tgtccgcgcc gcagcgttcg atgcccgcgc ccgcgtcctt cagcttgtcc
     5041 agcacggcgc gcatatggtc cgcgcgcgcg ccgcgcagct tgatctcgcc gttcgtggcc
     5101 acggcggcaa tcgcataggt ggccgcctcg atgcggtctg ggatcacctc gtgctccgcg
     5161 ccgtgcaatt cc
//
LOCUS       DATCGS010000504         6216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_192864, whole genome shotgun sequence.
ACCESSION   DATCGS010000504 DATCGS010000000
VERSION     DATCGS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192864"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..546
                     /locus_tag="VIL39_10350"
     CDS             208..546
                     /locus_tag="VIL39_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY5481871.1"
                     /translation="MSQTHPLTTTTFELPGYRAVKSFGVVRGIVVRSRSIVGNFGAHI
                     QSLIGGNISIYTSLCERTREEAFNQMITHAGELGANAVVGVRYDATEIGAGITEVLCY
                     GTAVYVERAG"
     gene            641..1390
                     /locus_tag="VIL39_10355"
     CDS             641..1390
                     /locus_tag="VIL39_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5481872.1"
                     /translation="MSGHSKWSKIKTFKGALDAKRGKIFSKLSKEITIAAKIGGGDPN
                     MNPRLRMVLLKCRSANMPRDNIERAIKKGMGGGETVNYEDLTYEVYGPNGVAVLVEVS
                     TDNRNRSASEIRSLVAKNNGTIASQGSVTRLFHRKGQIIIPRDAANEDQLMEVALEAG
                     AEDFKAEPQGYEIITDPAHFEAVHKQIEAKGIKADVAEMTALPTVTVPLPDDRAVAAV
                     NKLIEALEDHDDVKEVYSNAEFPDEEAKGGA"
     gene            1469..4606
                     /locus_tag="VIL39_10360"
     CDS             1469..4606
                     /locus_tag="VIL39_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5481873.1"
                     /translation="MKIKPLSNHNFIARFLTWVTGMILRHRRLFLYGQVVLFALSILI
                     TVKFPGIEFNTSRNDLVGSNKKYHQNFLRLKEEFPTQDDLVVVVQSENTEKNRQFVER
                     LGAKLETETNLFHDVFYKGDLKMLGSKALLFVPDKDLAELGQTLKDFRPFIAQFTRTT
                     NLVSLFNMVCAQFRTAKQEANAENDALIKALPALERIITQATASLRRQGTPPSPGITA
                     LFDPNGEADQQVYITFDKGRVFLVTAQAPTDALNGAAVVRIRQLVAETKAEVPGLNVG
                     LTGEPVLELDEMAQSQKDTTVACVVSLVLCALIFIYGYQETGRPLKATLCLIVGLGYT
                     LAFATLVIGHLNILTITFMPILIGLAIDYGVHLISRYEEELRRGMSEEASLTTAMVFT
                     GQGILTGGLTTAGGFLAMGFTNFKGIQEMGVICGGGLVVCLIPMMTMLPVLLLRGRQN
                     VADHEQGDLGKKRARIENFWLRRPAWTAGVTLALCALAATQIHKVYFDYNLLNMQSAG
                     LPAVEFEQELIDTTPKSVLFGAVVATNLQQAVWLEQQLTNLPAVSGVESMAGRLSEDQ
                     TARLALVGDIKEELSSVCFISPDPGPVNIQELSATLYSLRGYFGAALEEVQKPSSPMF
                     SVDDIVNLPSLATNLYQKAEGVSAFLSGRLEEPARQALGAYVSSKTNPDDLKSNLVQY
                     LNRIVAGQCIYETNRFQKVELGPDTKILLAQSQSPQGEALACLNRLLLEDAYPLGLSG
                     NEKALFALRRQLLSAREAIEGLLKEMLSGDLQRVTASSLKLAEFQRSLFNDVRDTFQA
                     LRSQDNRAPLSVADLPEALHDRFVGVTGKYLLMVYPKEDLWKRENQKAFIEQVGKVDP
                     NVTGTPVQLYHYTALLKDSYQEAAWYSLGAIVILVFVHFRSVTCVLLALVPVAIGSLW
                     LGGLMGWLRVPLNPANIMTLPLVIGIGVTNGIHILNRFAEEQTPSILGRSTGKAVLVS
                     GLTSMAGFGSLMLAQHRGIYSLGCVMTTGLATCMIAGLTFLPALLNLLMRSPAPTEQP
                     SADNARSTLGREEPR"
     gene            4879..>6216
                     /locus_tag="VIL39_10365"
     CDS             4879..>6216
                     /locus_tag="VIL39_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481874.1"
                     /translation="MSELPTAARADSLDAGLLFDEFDLTLALGHRTEAVGPFFYSEQK
                     ETQRTWAVPPLFSSTRDPATESKEFDFLYPVITYDRYGEQYRWQLFQMLSFAGSPTQQ
                     ESARDRFTLFPLYFQQRSSDPSQNYTALFPFYGHLKNRLFRDEIFFVMWPLYSQTRKR
                     DVVTDNYLVPIFSLRNGDGLRGWQVWPLAGKEHKDVTTQTNGFNDVKTIGGRDSFFVL
                     WPLFFIDKSGIGTENPQWQLASLPAFSLLRSPLRDSTTVIWPFFNYVEEREKKYREWD
                     APWPLIVFARGEGKHTTRVWPLFSRSHNATLEDNFYLWPIYKCDRAHSAPLDRQLSRI
                     CFFLFTDLKEMNTETEAVRRRIECLPFFRFHRDFNGNSRLQVLALLEPFLPASKSIDR
                     NYSPLWSLWRSENNPRAGATSQSLLWNLYRREATPATRKCSLLFGLFQYQSSPE"
BASE COUNT         1260 a         1761 c         1915 g         1280 t
ORIGIN      
        1 accatcatgc gacccccaag cccggggaaa gcgtcgggag agggggtgta gagggcgagc
       61 ctttggtggc gcgctggttc ggtgctcttg gggaaaggcg ggggctccgg gtcctgatcc
      121 ggccttctgg cttctccctg ccccaggcgt cggctgagaa aaataagctt ctgccgggag
      181 gcaaagtggt gttcaatttc cgccgttatg agtcaaacac atccgctgac gacaacgacg
      241 tttgagctgc ccggttaccg ggcggtcaaa tccttcggtg tggtgcgcgg tattgtggtg
      301 cgctcgcgct cgattgtggg caattttggg gcgcatatcc aaagcctgat tggcgggaac
      361 atctcgatct acacgtcgct gtgcgaacgg acgcgggagg aggcgttcaa ccagatgatc
      421 acccacgccg gggagctggg cgctaacgcg gtggtcggcg tccgttacga cgcgacggag
      481 attggcgcgg gtattaccga ggtgctatgc tacggcacgg cggtgtatgt cgagcgggcg
      541 gggtagttgc tagtgatttg agttgatggt ggatagtcgc ggaacacatc tgcttgtggt
      601 tcgaggctgt caactctcaa ctctcaacca ataactaaat atgtcaggac atagcaaatg
      661 gtcaaagatc aagacgttca agggtgcgct tgacgcgaag cgcgggaaga tcttctcgaa
      721 gctgagcaag gaaatcacca tcgccgccaa gatcgggggc ggtgacccga atatgaaccc
      781 gcgtctgcgg atggtgctgc tcaagtgccg ctcggccaac atgccgcgcg acaacatcga
      841 gcgggcgatc aagaagggca tgggcggcgg cgagacggtg aactacgagg atctgaccta
      901 cgaggtttac gggccgaacg gggtggcggt gttggtggag gtaagcacgg ataaccgcaa
      961 ccgcagcgcg tcggaaatcc gcagcctcgt cgccaagaac aacggcacca tcgcctcgca
     1021 aggctcggtg acgagactct tccaccgcaa ggggcagatc atcatcccgc gcgacgcggc
     1081 caatgaggat cagttaatgg aagtggcgct ggaggcgggc gcggaggatt tcaaggcgga
     1141 gccgcaaggg tatgagatca ttaccgaccc ggcccatttt gaggccgtcc acaagcagat
     1201 cgaggccaaa ggcatcaagg ccgacgtggc ggaaatgacg gcgctgccga cggtcacggt
     1261 gccgctgccg gacgaccgcg cggtcgccgc ggtgaacaag ctgatcgagg ctttggaaga
     1321 ccatgatgac gtgaaggagg tctattcgaa tgccgaattc ccggatgaag aggccaaggg
     1381 cggggcttga attcgggcag agaggcccgg ggtcgttaat ccgtgcttgt tcagagtcca
     1441 tccgcatctt taggggccag tcgcgccgat gaagattaag ccgctcagca atcacaattt
     1501 tatcgcgcga ttcctgacct gggtgaccgg catgattttg cgtcatcggc ggctgttcct
     1561 ctatgggcag gtggtgttgt ttgctctgtc cattctgatc acggtcaagt tccccggcat
     1621 tgaattcaat acgagccgca acgacctggt gggctcgaac aagaagtatc atcagaactt
     1681 cctgcggctc aaggaggagt tcccgacgca ggatgacctg gtggtggtgg tccagagcga
     1741 gaacaccgag aagaaccggc agttcgtgga gcggctgggg gccaagcttg agacggaaac
     1801 caatttgttc cacgacgtgt tctacaaggg cgacctgaag atgcttgggt ccaaggccct
     1861 gttgttcgtg cccgacaagg acctggccga gttgggccag acgctgaagg atttccggcc
     1921 attcatcgcg caattcaccc gcacgacgaa ccttgtctcg ctcttcaaca tggtctgcgc
     1981 acagttccgg acggccaagc aggaggccaa cgccgagaac gacgcgctga tcaaggcgct
     2041 gccggcgctg gaacgcatca taacccaggc cacggccagc ctgcgccgcc agggcacccc
     2101 gccttcgccg ggaatcaccg cgctgttcga tcccaacggg gaggcagacc agcaggtcta
     2161 tatcactttc gataaaggac gcgttttcct ggtgacagcc caggcgccaa ccgatgcgct
     2221 gaacggcgcc gcggtggtgc ggatacggca gttggtggcg gaaaccaagg ccgaggtgcc
     2281 aggccttaac gtcggcttga caggggaacc ggtgctggag ttggatgaaa tggcgcaatc
     2341 gcagaaagac acgacggtgg cctgtgtggt gtcgctggtg ctctgcgcgc tcatcttcat
     2401 ttatggctat caggagacgg ggcggccact gaaggcgacg ctctgcttga ttgtcggcct
     2461 gggttacacg ctggcctttg cgaccctggt catcggccac ctgaatattc tgacgatcac
     2521 cttcatgccg atcctgatcg gcctggcgat tgactatggc gtgcatttga tttcccggta
     2581 tgaggaggag ttgcgccggg gcatgagcga ggaagcctcc ttgactacgg ccatggtctt
     2641 caccgggcag ggcattctga cgggcgggct gaccaccgcc ggcgggttcc tggcgatggg
     2701 gtttaccaat ttcaagggca tccaggaaat gggggtcatc tgcggcggcg gattggtcgt
     2761 ttgcctgatt cccatgatga ccatgctgcc ggtgttgctg ctgcgcggcc gccagaatgt
     2821 ggcggaccac gagcagggcg accttgggaa gaagcgggcg cgaattgaga acttctggct
     2881 gcggcgacca gcctggacgg ccggcgtgac gctggcgctc tgcgcgttgg cagcgacgca
     2941 gatccacaag gtgtattttg attacaacct gctcaacatg cagagcgcgg ggttgccggc
     3001 ggtggagttc gagcaggagc ttattgatac gacccccaag tctgtcttgt tcggggccgt
     3061 cgtcgccacc aacctgcagc aggccgtctg gctcgaacag cagcttacga atcttccggc
     3121 agtttccggc gtcgaatcca tggcgggtcg gctgagtgaa gaccagaccg ccaggctggc
     3181 gctagtgggt gacatcaagg aggaactttc gtccgtctgc tttattagcc ctgaccccgg
     3241 gccggtcaat atccaggaac tgagcgccac gctctactcc ctccgcggct acttcggcgc
     3301 ggcgctggaa gaagtccaga aacccagttc cccgatgttt tcagtggatg atattgtgaa
     3361 tctgccctcg ttggctacga acctctacca gaaggcagag ggtgtttccg ccttcctgag
     3421 cggacgattg gaggaaccgg ctcgccaggc tctgggagcc tacgtgagct caaaaaccaa
     3481 ccccgatgac ctcaaatcca acctggttca gtatcttaac cggatcgtcg caggccagtg
     3541 catctacgaa acaaaccgtt tccagaaggt tgaactggga ccagacacaa agatactttt
     3601 ggcgcaaagc caaagccctc agggagaagc tctggcctgc ctaaaccgtc tgctcctgga
     3661 ggatgcttat cccctcggac tgtcgggaaa cgagaaagcg ctcttcgctc tgcgccggca
     3721 actcctttcc gcgcgcgagg ccattgaagg gctgctcaag gaaatgttgt cgggcgacct
     3781 ccagcgagtc accgcgagtt ccctcaagtt ggctgagttc caacgaagcc tgttcaacga
     3841 cgtccgcgac acgttccaag ccttgcgaag tcaggacaac cgcgccccgt taagcgtggc
     3901 ggatttgccg gaggcgttgc atgaccggtt cgtcggtgtc acgggcaagt acctgctgat
     3961 ggtctatccc aaggaagacc tgtggaagcg ggagaatcag aaggcgttta ttgagcaggt
     4021 ggggaaggtt gatccgaacg tcaccgggac gccggtgcag ctttatcatt acacggcgct
     4081 gctcaaggac agttaccagg aagcggcgtg gtattcgctg ggggcgattg tgattctggt
     4141 gttcgtgcat ttccgcagtg tcacctgcgt cctgctcgcc ttggtgccgg tggcgattgg
     4201 ttccctgtgg ctgggcggcc tgatgggatg gctgcgggtg ccgctcaacc cggcgaatat
     4261 tatgactctg ccgctggtta tcgggatcgg cgtgacgaat ggcattcaca tcctcaaccg
     4321 gtttgccgaa gaacagacgc cgagtatcct ggggcgcagc accgggaagg cggtgctggt
     4381 ctctggcttg acgtccatgg ccggcttcgg gagtttaatg ctagcccaac atcggggcat
     4441 atacagcctg ggctgcgtga tgactaccgg cctggcgacc tgcatgatcg cgggactgac
     4501 gttcctgcca gcgctgttga acctgctgat gcgctcgccg gcgccgacag aacaacccag
     4561 tgccgacaat gcacggtcga cactgggtcg ggaggaaccg aggtaaaaaa ccccagatga
     4621 gaaaataata gaggaatacc cttgggtgtc aatccttatg tttagagagt tttctggcaa
     4681 aatgggcatt ccattaacgt gattaccccc agcccaaagc gcttgacgcc acgctcgtct
     4741 gttctacgct catttgttct ggcaatgaga catactcggg aattggccct cgccccgcaa
     4801 aaactacagt ctctgtggtt cgtccccgca cgcgcatgtg cctcgcttta tattgtcctc
     4861 tcttgtctga gcctggcatt gagcgaactt ccgactgccg caagggctga ctccctcgac
     4921 gccgggttat tgttcgatga gttcgacctg acgcttgcgc tcgggcaccg gacggaggca
     4981 gtgggtcctt tcttctattc agagcaaaag gagacacaac ggacctgggc ggtgccccct
     5041 ttgttctctt ccacgaggga tccggcgacg gagtccaagg aattcgactt cctctatccc
     5101 gtgataacgt atgatcgcta cggcgagcaa taccgttggc aattgtttca aatgctgagt
     5161 ttcgctggca gtcccacgca acaggagagc gcccgggacc gcttcaccct gttccccttg
     5221 tacttccagc aacgctcctc agacccgagc cagaactaca ccgcgctttt ccctttctat
     5281 ggccacctga aaaaccggct cttccgggat gagatcttct tcgtcatgtg gcctctctac
     5341 agccagacac gaaagaggga tgtggttacg gacaactacc tggtgcccat ttttagtctc
     5401 cgcaacggcg acgggttgcg tggttggcaa gtgtggccgc tggctgggaa ggagcacaaa
     5461 gacgtcacca cccagacgaa cggtttcaat gatgtcaaga ccatcggcgg gcgcgacagt
     5521 ttctttgtgc tctggccgct cttcttcatc gacaagagcg ggatcggcac cgagaacccg
     5581 cagtggcagc tcgcttcact gcctgccttc agcttgctcc gttcaccgct gcgggactcg
     5641 accaccgtta tttggccgtt cttcaactat gtggaggagc gggagaagaa ataccgcgaa
     5701 tgggatgcgc cgtggccgct catcgtgttc gcgcgtggcg aaggcaagca caccacgcgg
     5761 gtctggcccc tcttcagccg gtcccacaat gccaccctgg aggacaattt ctatctttgg
     5821 ccgatttata agtgcgaccg ggcgcattcg gcgccacttg accggcagct cagccgcata
     5881 tgcttcttcc tattcaccga cctcaaggag atgaacaccg aaaccgaggc cgtccgtcgg
     5941 cggatcgaat gcctgccgtt cttccgtttc caccgcgatt tcaatgggaa tagccggttg
     6001 caggtgcttg ccctgcttga accgttcttg cccgcgagca agagcattga ccgcaattac
     6061 tcgccgcttt ggtcgctctg gcgttccgag aacaatccca gagccggcgc caccagccaa
     6121 tcgctgttgt ggaacctgta ccgacgcgaa gccacacctg ccaccagaaa gtgctcgctc
     6181 ctgttcgggc ttttccagta tcaatccagt cccgaa
//
LOCUS       DATCGS010000505         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_636878, whole genome shotgun sequence.
ACCESSION   DATCGS010000505 DATCGS010000000
VERSION     DATCGS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636878"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VIL39_10370"
     CDS             <1..947
                     /locus_tag="VIL39_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5481875.1"
                     /translation="AQLCLDKLAMRQHFLERIGPQSTGRFQEVQTEEQLRGFAAEVGF
                     PLVLKPTNLSASLFISLNHSLDELLASSRRLVAEVPGYYQRAALKGKQLAIQVEEFMT
                     GSNHSIDCWVDATGRVSATPVVDVLTGKDVGIDDFHHFARITPSQLSREEEAEASRLA
                     MAGAQALNMSTVIGHVEFIQTPAGPRLLEIGARPGGNRIRILEMTYGIDQIFAYYQIL
                     CGGSPEMRREKEQPSAIVTPFPRGQGILSSFHGLDRLQKLPTYCYHEVRGRVGQKVGL
                     AKDGFKAPLYIELTAPTRDEVRRDVDRIASWNDLIVVQ"
     gene            949..2217
                     /locus_tag="VIL39_10375"
     CDS             949..2217
                     /locus_tag="VIL39_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5481876.1"
                     /translation="MSQRSQATRAVWLACFGIGLLFLARGAFLPYFFPIFEHLTRLSY
                     GEISLLLNLYVFSQSLCAPLAGWYTDRTSVPTAVFTAILLGSLGFLVVLQAHTFALIA
                     LAMAAIGSGFVLGKIGFNTLLVDSSSQEELRRSIAGRAMILNLGSFAGNALALQVIEG
                     LGYKPLVILLLAANFTLGIACLAPRIPAHTGHPTMKLGDLRTVIRNKPFLADSLRLFS
                     IYVPYGCWGTIIPKYVIDLYGSKEPVRYIYLVSLCTLLGGSYAIGSWLAPKLYRWGFQ
                     WRWWTLVSLLCFCCGLLLLSLGGNRVLLGVAVAVFICGEILMTPCFAETAKTHAQAGK
                     TGTYQGVLHVFEGGGRVVGSMTALAVYGWLQGSVLARFYWVIMAPAFLLFSGTIHWWA
                     YRATRPASAGQVERAVTSSTGCVDADELEP"
     gene            2231..>3627
                     /locus_tag="VIL39_10380"
     CDS             2231..>3627
                     /locus_tag="VIL39_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5481877.1"
                     /translation="MKHLLVGAQLCFMASSVMAAAATFDRFTDTFAAEWMRADPQAAS
                     ALQYFSGAEQDALDRQLTPITKAARAARVAAARRGLAELRRLDRASLDSTQCTSAATL
                     EWQLDDIVRAEPFADYAFVFQQYNGLQVQLVNFLTQTHPLRSRRDIENYLARLELVAG
                     QMDEGIAQARERATRGILPPRFILSATIQQFDRFLAAAPRQNVFVASLNERAAKVKDL
                     PAGDRAKFLDVAEHTVSLSIIPAFRRARALLQEQLPGSADDAGLWRFPDGDKAYAQAL
                     HRHTTTDYTAAQIHERGLKEVARIEKQMDELLRQLGYNDGSVKERMQKLDAESQPRTE
                     PDPRPALLAKYDEILRDAEKRAALIFDLRPKAPVVVKREPAFTEQNAAAHYTIPAPDG
                     SRPGIFWAPLPDPTFRITEMRTLVYHEAVPGHHFQVALQSEMASLPRFRRDRVFGSIP
                     AHGEGWALYAEQLAA"
BASE COUNT          642 a         1174 c         1061 g          750 t
ORIGIN      
        1 cggcccagct ctgcctcgac aaattggcca tgcgccagca ctttctcgaa cggatcggcc
       61 cccagtccac gggccggttc caggaagtgc agacggagga gcagttgcgg ggttttgccg
      121 ccgaggtagg cttcccgctg gtcctcaagc cgaccaacct ctccgccagc ctgttcattt
      181 ccctgaacca ttccctggat gagttgctgg cgtcatcccg gcggctggtc gccgaagtgc
      241 cgggctatta ccaacgggcc gccctcaaag gcaagcaatt ggcaatccag gtcgaggagt
      301 tcatgactgg cagcaatcat tcgattgatt gctgggtcga tgccacgggc cgcgtttcgg
      361 ccacgccggt ggtggatgtg ctgaccggca aggatgtggg gattgacgac ttccatcatt
      421 tcgctcgcat taccccttcg caattgagcc gagaggaaga agccgaggcg tcccggttgg
      481 cgatggcggg ggctcaggcc ctgaacatgt ccacggttat tggccatgtg gagtttatcc
      541 agacgccagc cggccctcgg ttgctggaga ttggggcgcg acctggtgga aaccggatcc
      601 gtattctgga gatgacctac gggattgatc agatcttcgc ctactatcag atcctctgtg
      661 ggggaagtcc ggaaatgcgg cgggagaagg agcagcccag cgcaatagtg actccttttc
      721 cccgaggcca gggaattctg agttccttcc acggcctgga ccggctccaa aagctgccca
      781 cttactgtta ccatgaggtc cggggccggg tgggccagaa ggtcggcctg gccaaggatg
      841 gattcaaggc cccgctttac atcgaactga cggcgcccac ccgggacgag gtccgccggg
      901 acgtggaccg gatcgcctcc tggaacgact tgattgtcgt gcaataaaat gagccagcgc
      961 tcgcaagcca cgcgtgccgt ttggctggcc tgctttggca tcggcctgct cttcctggcg
     1021 cggggggcct tcctgccata cttcttcccg atcttcgagc acctgacccg gctctcctat
     1081 ggcgagattt ccctgctgtt gaatctctac gtcttttccc agtccttgtg cgcgcccttg
     1141 gcaggctggt acaccgatcg gacctctgtg cccaccgcgg tattcacggc catcttgctc
     1201 ggttcgctcg gtttcctggt ggtgctccag gcccatacgt tcgcgctgat tgccctggcc
     1261 atggcggcga tcggctccgg gtttgtcctg ggcaaaatag gctttaacac ccttttggtg
     1321 gacagctcca gccaggaaga attgcgccgg agcatcgctg gccgggccat gattttgaac
     1381 cttggctctt ttgccggcaa cgccctggcg cttcaggtaa tcgagggatt gggctacaaa
     1441 cccctcgtga ttctcctgct cgccgccaac ttcacccttg ggattgcctg cctggcgccc
     1501 cgtattcccg ctcacaccgg gcacccaacg atgaagctcg gcgacctaag gaccgtgatt
     1561 cggaacaagc cctttctggc cgatagcctg cgtctgttct ccatctatgt gccgtacgga
     1621 tgctggggaa cgatcattcc gaagtatgtc attgacctat atggctcgaa ggaacccgtg
     1681 cgatacattt atctcgtcag cctgtgcacc ttgctgggcg gatcttacgc catcggaagc
     1741 tggctcgccc cgaagctcta ccgttggggc ttccaatggc gatggtggac cctggtgtct
     1801 ctgctctgtt tctgctgcgg cctcctgttg ctcagcctcg gggggaaccg cgtgctgctt
     1861 ggggtagcgg ttgcagtttt tatttgtggt gagatcctca tgacaccttg tttcgctgag
     1921 acggccaaga ctcacgccca ggcaggcaag accggaacgt accagggagt cttgcacgtc
     1981 tttgaaggcg gcggccgagt cgtggggtca atgaccgcgc tggcggttta tggttggctg
     2041 caaggctcgg tcctcgcccg cttctactgg gtgatcatgg cgccggcttt tctcctcttc
     2101 tccgggacca tccactggtg ggcctaccgg gcgaccaggc ctgcttccgc tggccaggtg
     2161 gaacgcgcgg tgacttcttc aacggggtgc gttgatgccg atgaactgga accgtaaagc
     2221 tggcgatcca atgaaacacc tgctcgttgg tgctcagctt tgttttatgg cgtcgtctgt
     2281 catggctgcc gccgcgacct tcgaccgctt caccgacacc ttcgctgccg agtggatgcg
     2341 cgccgaccca caggcagcaa gcgccctgca atatttcagc ggcgccgagc aagatgcgct
     2401 cgaccgccag ctcacgccca ttaccaaggc ggcccgcgcg gcccgtgtcg ccgccgcacg
     2461 gcgcggcctg gctgaactga gaagattgga tcgtgccagc ctcgactcca cccagtgcac
     2521 ttccgccgcc acactcgaat ggcaactcga cgacatcgtg cgcgcagagc ccttcgctga
     2581 ctacgccttc gttttccaac agtacaatgg gctccaggta caactcgtca acttcctcac
     2641 ccaaacgcac ccgttgcgca gccgccgcga catcgaaaac tatctcgcgc gcctggagtt
     2701 ggtggccggc caaatggacg aggggattgc tcaagccagg gaacgcgcca cccgcggaat
     2761 tctgccaccg cggttcattc tcagcgctac catccagcag tttgatcgct ttctcgccgc
     2821 cgcgccgcgc cagaatgttt tcgtcgcttc gctcaacgag cgcgccgcga aagtgaaaga
     2881 cctccccgcc ggtgaccgcg caaagttcct cgacgtcgcc gagcatacgg tctccttgtc
     2941 aatcattccc gccttccgtc gtgcccgagc gctgctgcaa gaacagttac cggggtcagc
     3001 cgacgatgcc ggcctctggc gttttccgga tggcgacaaa gcctacgccc aggcactgca
     3061 ccgacacacc acgaccgact acaccgccgc ccaaattcac gagcgcggcc taaaggaagt
     3121 tgcgcgcatc gagaaacaga tggacgagct attacgccag ctcggttaca acgatggcag
     3181 cgtcaaggag cgcatgcaaa aactggatgc agagtcgcag ccgcggaccg aacccgaccc
     3241 gcgcccggct ctgcttgcga aatacgacga gattctccgc gacgccgaga agcgcgccgc
     3301 cttgatcttc gatttgcgac caaaggcgcc cgtcgttgtg aaacgcgagc ccgccttcac
     3361 tgagcagaac gccgccgccc actataccat cccggcgccc gacgggtcac gcccgggcat
     3421 cttctgggcc ccgctgcccg acccgacctt tagaattacc gagatgcgca cgctcgttta
     3481 ccacgaagcc gtgcccggcc atcattttca ggtcgcgctc cagagcgaga tggccagcct
     3541 gccgcgcttc cgtcgcgatc gcgtgttcgg gtctattccc gcgcacggcg aaggctgggc
     3601 actctatgcc gagcagcttg cggctga
//
LOCUS       DATCGS010000506         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_41230, whole genome shotgun sequence.
ACCESSION   DATCGS010000506 DATCGS010000000
VERSION     DATCGS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41230"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..833
                     /locus_tag="VIL39_10385"
     CDS             210..833
                     /locus_tag="VIL39_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481878.1"
                     /translation="MNNREVGVSLDLAKNAKSEWIASMGLPSQNMTGLVVMDVAVNGK
                     SVKFVAVELQMATCDLTLGPDGRMKGTMSAPQGPMSTQGPMPVELKRTGEAKVELIPG
                     SPAVSKELEGDWEGSLQGPNGVLLPLVFHFKNQPDKTVAATIDSPDRNGMAMPLNDVK
                     QTGQKVEIGLKIAGGSRFQGSLNKEGTELAGELIHDASSMPLTLRKK"
     gene            902..1360
                     /locus_tag="VIL39_10390"
     CDS             902..1360
                     /locus_tag="VIL39_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481879.1"
                     /translation="MLALLAAVLAATAAAQQAKPIANWVNLNQLVAGSEIRVTLAAGK
                     TMRGFVQRVTAESLAIDATTSQEMLSRQDIRRVELKKPGHRGRNTLIGLAIGTGAGLA
                     IGAGVDAKSGPGVMFPDLGKVVFSSAGVLLGTVIGVALPTGGWRDVYRAP"
     gene            1509..1919
                     /locus_tag="VIL39_10395"
     CDS             1509..1919
                     /locus_tag="VIL39_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481880.1"
                     /translation="METEREGKKAAITVIRWWARVLTVISVIFAGLLWLVAIGEATRP
                     FKPLAAIWFGLSGIYAVAMCLALKWERAAVLVGVAALLAAHVEGLFLGLFEYPGHLTR
                     VSVVNPFLLVFWVPVLLYLRCWRLEGRGRRKAVL"
     gene            complement(2027..2212)
                     /locus_tag="VIL39_10400"
     CDS             complement(2027..2212)
                     /locus_tag="VIL39_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481881.1"
                     /translation="MSIAGYVSRAMLSRYSHVRMEAKRRALDEIAARQCAADEKRKGD
                     AERQMQATVTPLTAAVQ"
BASE COUNT          511 a          777 c          906 g          535 t
ORIGIN      
        1 acgaaaactc cgggcacaat ccattagtgt ccgggatggc aaaacggaaa tctagctgag
       61 ttggcacaag aaaggagttc caaatgacac ttcaggcgaa tcacatcttt ttgactcggt
      121 ctcttcgtgg cgcgggcgcg gctgttctct ttgcgtcgct tgtgtgcgcg caagcaggcc
      181 ccgacggtca ctgggaaggt tcctttaccg tgaacaatcg cgaggtcggg gtgtcgctcg
      241 acctggcgaa gaacgcgaaa tcggagtgga tcgccagtat gggccttccc tcccaaaaca
      301 tgaccggcct tgtcgtgatg gatgtggcgg taaacggcaa gtccgtgaag tttgttgccg
      361 tcgaactcca gatggctacg tgcgacctga ccctgggccc ggacgggaga atgaagggga
      421 cgatgtccgc cccgcagggc ccgatgtcca cgcagggccc gatgccggtc gaactcaagc
      481 gcacgggcga ggcaaaggtg gaactgatcc caggcagccc agcggtgtcg aaggaactgg
      541 aaggagattg ggaaggctcg ctccaggggc ccaacggtgt gttgctcccg ctggtattcc
      601 acttcaaaaa ccaaccggac aagacggtag cggcgaccat agacagtccc gacaggaacg
      661 gcatggctat gccgctcaac gacgtgaaac agacgggcca gaaggtggag atcggattga
      721 agatcgctgg cggcagcagg tttcagggaa gcctgaacaa ggaaggtacc gaactggccg
      781 gtgagttgat ccacgacgca agcagcatgc cgctgacgct acggaagaaa taggtgctac
      841 gtgcgtggac gcatagtggc cagaaagtct ttccatgacc aggcgattga atcgcatctg
      901 cttgttggcc ttattggccg cggtcctcgc ggcgaccgca gcggcccaac aggccaagcc
      961 aattgcgaat tgggtcaacc tcaaccaact tgtcgcggga tcagagatcc gcgttaccct
     1021 tgcggccggg aagaccatgc gcgggttcgt gcagcgcgtg actgccgagt cactggcgat
     1081 cgacgcgacc accagccagg agatgctttc ccggcaggac atccggcgcg tcgaactgaa
     1141 gaagcctggg catcgcgggc ggaacacgct cattggattg gcgattggaa ccggcgccgg
     1201 ccttgccatt ggcgctggcg ttgatgcgaa gtcgggcccc ggagtgatgt ttccggacct
     1261 tggcaaggtg gtgttttcgt cggcgggagt gctcttaggc acggtcatcg gagtggcatt
     1321 gccaactggt ggatggcgcg acgtgtatcg cgcgccatga gcccggcggc gggctgaatc
     1381 ccggcgggga ccgaactaac gccggcgggt tgggtattag gtcaagtcac gactgctggt
     1441 ccgcggtgaa cttgggtccg gattgaccga tgagcgagtg tggctgtttt tatagaatgg
     1501 aggactccat ggagacggaa cgggaaggga agaaggcagc aataacggtt atcaggtggt
     1561 gggcccgcgt gctcacggtg atctctgtga tctttgcggg gcttctctgg ctcgtggcca
     1621 tcggcgaggc tactaggcca tttaagccac tggcagcgat ctggtttggc ctgtcgggga
     1681 tctatgccgt cgcgatgtgc ctggcgctca aatgggagcg cgcggccgtg ctcgttggcg
     1741 tggcagccct cctcgcagcc cacgttgagg ggctgttctt gggactgttc gaatatcccg
     1801 gtcacctcac tcgcgtaagt gtcgtgaacc cgttccttct agttttctgg gttcccgtcc
     1861 tgttgtactt gcggtgctgg cgactcgaag ggcggggtcg gaggaaggcg gtcctctaaa
     1921 cgtctgggga ggccctggga ttcgctcctg gcggacggcg ttaccgtaga agcggaccaa
     1981 ccgagtccac aatccggcgg tcaacggcgg gtttccggcg ggcggactac tgaaccgccg
     2041 ccgttagtgg agtcaccgta gcctgcatct gccgttcggc gtcccctttg cgcttctcat
     2101 ccgccgcgca ctgccgcgcg gcgatctcgt cgagggccct ccgtttcgct tccatccgca
     2161 cgtgggaata gcgcgagagc atggcgcgcg agacgtaccc ggcgatgctc atgatcacct
     2221 cgtcgcctgc gcctcttctc gcgtgccacc accaagccag gccgccggtc acgacgccac
     2281 acgtgcgccg acggcggcaa cctggcgccg tcctaccact tggcggcgcc gtcgcgccgg
     2341 tggcgcccat tgcgcgaacg cgggccaatt ctggcgagcc aactgccgaa cagcatggtt
     2401 tggtgtttat tggggcgact ctgctggagc tttgcgcgtc atgacccgtc atgacccgtt
     2461 gcccgtaagt ctatgagaca ttggtagcga taacgggagt cgaacccgtt aacaccagtt
     2521 catcaatgcc accgcgagtg ccaacttggc cctggcagga ctggtggtcg ggccgtagct
     2581 ccggctgcaa atgtgatgtc aatggtccct gacatcacgg ccggcaccgg caccgaatct
     2641 gacgagacac ctggttggtg gtggcctgtg gggctgtcgc agtcggaagg agcacacgag
     2701 atggccgttt ccgtcgatgg ggctctttt
//
LOCUS       DATCGS010000507         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_388412, whole genome shotgun sequence.
ACCESSION   DATCGS010000507 DATCGS010000000
VERSION     DATCGS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388412"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VIL39_10405"
     CDS             complement(<1..938)
                     /locus_tag="VIL39_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY5481882.1"
                     /translation="MPNSNAKVGRAARPAFLKPALAAVILMAVCAAVLFSQSAPNTPV
                     VTGNARVDRLLSQMTLEEKIALLHGGTEASPSGVGQAGTWPGLPRLGIPSLRLTDGPP
                     GISIGIWSTGMTATMGLAATWSREDAKKNGIVIGRDARTLGQDVVLQPFVNLVRDFEF
                     GRAYNTFGEDPFLTGQIAVAQVQGTQGEGVMSQAKHFVGYDGGNDVYVDPQTLREIYI
                     AQFVDLANAGVSSIMCSYNKINGLQACDNNEIQNIILRGEDGFRGFNTSDWGATHGTL
                     NIANGLDMEMPGGSYTAADPGGRGMGGGGGRGAAPAG"
     gene            complement(1008..1796)
                     /locus_tag="VIL39_10410"
     CDS             complement(1008..1796)
                     /locus_tag="VIL39_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HEY5481883.1"
                     /translation="MRRLVVMSIAVFAQCVAFGQAPSLQPAFEVASVKTAGPPREEPG
                     GARSTGMPPRTFGDPGLVNYSSVTLKGVLCRAYNLLPDKIVGPSWLDSERYDIVAKVP
                     KGAPPGQIPSMLQNLLAERFRMSVHWETKQTPGYALVVGKSGPKLTKSALEDATRRSF
                     GFNGSGHLAWKGETLADVATVLSTFLDRPVVDMTEIPGIFDITLDAAPDSLPGLHFGA
                     PSEDSASFPSIFAAIRGLGLNLEPREVPVKQLVVDSAQKIPTPN"
     gene            2073..2369
                     /gene="rplU"
                     /locus_tag="VIL39_10415"
     CDS             2073..2369
                     /gene="rplU"
                     /locus_tag="VIL39_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5481884.1"
                     /translation="MYAIIETGGKQYRVAPGDVIRVEKLTGDAGSEVELPVKAAFKDG
                     GELVAGGGTVKATIVGDGRGKKIIVFKFKRKKQYKKTIGHRQSFTDVKVGDIGA"
     gene            2378..>2617
                     /gene="rpmA"
                     /locus_tag="VIL39_10420"
     CDS             2378..>2617
                     /gene="rpmA"
                     /locus_tag="VIL39_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5481885.1"
                     /translation="MAHKKGLGSSRNGRDSCAQRLGIKVFGGQLVPGGSIIVRQRGTR
                     IKPGLNVGWGKDDTLFAKITGVVEFRDRGRMGKFVN"
BASE COUNT          478 a          811 c          826 g          502 t
ORIGIN      
        1 cctgccggag ccgcgccccg tccgccgcct ccacccatgc cgcggcctcc aggatcggca
       61 gcggtatagc tgccgccagg catttccatg tcgaggccgt tggcgatgtt cagggtgccg
      121 tgggtggcgc cccagtccga cgtattgaag ccccggaaac catcttcacc ccgcaagatt
      181 atgttctgga tctcgttgtt gtcgcaggct tgcaggccgt tgatcttgtt gtacgagcac
      241 atgatcgaag aaacaccggc gttggcgagg tccacgaact gcgcgatgta gatctcgcgc
      301 aaggtttgcg ggtcgacgta gacgtcgttg ccgccgtcgt agccgacgaa gtgcttggct
      361 tgcgacataa cgccttcgcc ctgcgtcccc tggacctgcg ccacggcgat ctgccccgtc
      421 aggaacggat cctcgccgaa cgtgttgtag gcgcgtccga actcgaagtc gcgcaccagg
      481 ttgacgaacg gctggagcac cacgtcctgg cccagcgttc gggcgtcgcg gccaatcacg
      541 atgccgttct tcttggcgtc ttcgcggctc caggtggctg ccagtcccat ggtggccgtc
      601 atgccggtcg accagatgcc gatcgagatt cccggcggcc cgtcggttag ccgaagggag
      661 ggaatcccca gccgcggcag gccgggccag gttccggcct ggccgacgcc gctcggcgac
      721 gcctccgttc cgccgtgcag cagggcgatc ttctcctcca gcgtcatctg gctgaggagt
      781 ctgtccaccc gcgcgttccc cgtcaccacg ggcgtgtttg gcgcggattg agaaaagaga
      841 acggccgcac agacggccat gaggattaca gcggccaggg ctggcttcaa gaaggcaggc
      901 cgggcggccc gccccacttt ggcgttggaa tttggcatat tagtctccgg atggctgcca
      961 ttgtagctcc cggcggcggg cgtgaaacgg ccaccagaat catcgcttca gtttggcgtg
     1021 gggatctttt gcgccgagtc gaccaccagt tgtttgacgg gcacttctcg cggctccagg
     1081 ttcaagccca gtccccgtat cgcggcaaaa attgaaggaa acgaagcgga gtcctcggag
     1141 ggggcgccga aatgcagccc cggcagtgaa tcgggcgcag cgtcgagggt gatgtcgaat
     1201 atccccggta tttcagtcat gtccaccacg ggtcgatcta agaaggtgga taggaccgtc
     1261 gcaacatcgg ccagcgtttc ccccttccat gccaggtggc ccgacccgtt gaacccgaag
     1321 ctgcgccttg tcgcgtcctc cagggcgctt ttcgtgagct ttggtccgga cttgcccaca
     1381 acgagcgcat accccggggt ctgcttagtt tcccagtgca cgctcatccg gaatctctcc
     1441 gccaggagat tctgcagcat ggagggaatt tgccccggtg gagcaccctt cgggaccttg
     1501 gcgacaatgt cgtatcgctc gctatcgagc caggacggtc ccacgatttt gtctggcagc
     1561 aggttgtacg ccctgcacag aacgcccttc agggtcacac tggaatagtt gacgaggccc
     1621 gggtcgccaa atgtccgcgg cggcatgcct gtcgaccggg cgcctcccgg ctcttcacgc
     1681 ggcggaccag cagttttcac tgaagctacc tcgaaagcgg gttgcagaga cggcgcttgg
     1741 ccaaatgcca cacattgcgc aaaaacggct atggacatca ctacgaggcg tctcatctct
     1801 cgactccgcc gagccagaac tccttcacct tacgcgattc tctcctttcc ggcaagcccc
     1861 tgatatggcg ctgcggcggc ctcttgagaa tcgcttacag taggggtcgg gcatgcccga
     1921 ccctccaggg gcgaggcact ggttggcccc ctgggcaagg cattgtgcgc ttcatgccat
     1981 ttgatgccgg cctgcattct gtagtacact cggtatttac tccagtggtt ccgacacgcc
     2041 tccgcgtgtt ccggctccca atcgaggatt gcatgtacgc gataattgaa actggcggaa
     2101 aacagtaccg ggtggctccg ggcgacgtga tccgcgtgga aaaactgacc ggcgacgccg
     2161 gctcggaagt cgaactgccc gtcaaggcgg ccttcaagga tggaggcgaa ctggttgcgg
     2221 gcggcggaac ggtgaaggct accatcgtcg gcgacggccg gggcaagaag atcatcgtct
     2281 tcaagttcaa gcggaagaag cagtacaaga agaccatcgg gcaccgccag tccttcaccg
     2341 atgtgaaggt cggcgacatt ggggcatagg gtgacgcatg gctcataaaa aaggattagg
     2401 cagttccaga aacgggcgcg actcctgcgc gcagcggctc ggcatcaagg tcttcggcgg
     2461 ccagttggtc cccggcggtt cgatcatcgt gcggcagcgc ggcaccagga tcaagcccgg
     2521 ccttaacgtc ggctggggca aggacgacac gctgtttgcg aagatcaccg gcgtggtgga
     2581 gttcagggac cgcggccgga tgggcaagtt cgtcaac
//
LOCUS       DATCGS010000508         4251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_588022, whole genome shotgun sequence.
ACCESSION   DATCGS010000508 DATCGS010000000
VERSION     DATCGS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588022"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VIL39_10425"
     CDS             <1..743
                     /locus_tag="VIL39_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5481886.1"
                     /translation="ACMARGFVGDACLIPEPFEQKILTAEVGVLWMRADVFGSAGHTR
                     AASTMVNAIDKAYQLVAAMRQLEQQWNQERHPAFADQPHPLNFNLGLIKGGNWPSSVP
                     AHCEFSMRLSMYPGVDPEKAKRQVQAHIDQFCADDPWLKANPPEITWYGHHDWGVYEP
                     NPERNPLMQSVARAHTDVTGTPVEYHNSTACTDVRFWTEHWHKPSTCYGPIGEQLHAA
                     NEWVSLKSVSETTKVVAATVIDWCGVAH"
     gene            761..2101
                     /gene="gabT"
                     /locus_tag="VIL39_10430"
     CDS             761..2101
                     /gene="gabT"
                     /locus_tag="VIL39_10430"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY5481887.1"
                     /translation="MAKTVSIVTPVPGPKSRELLVRREAIVPRGVGNVAPIFAARAHG
                     ATITDVDGNTFIDFAGGIGVVNVGHTHPEVNDAIHAQVDKFLHTCFHVTMYEGYVRLA
                     EELARLTPGDFRKKTLLVNSGAEAVENAVKIARRFTGRPAVVTFENAFHGRTLLTMSL
                     TAKASTYKHGFGPFAPEIYRAPFPNTYQSPFASEAEAVEWAFTGFRRLVETEIGQDKV
                     AAVILEPVQGESGFLVTPPAFMKQLVAYCNKNGILFIADEIQTAFGRTGKWFAVEHYG
                     VTPDLFTIAKSMGAGLPIAAVVGRAEVMDAPQVGGLGGTYGGNPVACAGALKTIEVMA
                     RDGYNEKGAAIGKKVHARFRDFQKKYALVGDVRGLGAMVAMELVIDRRTKEAAAEKAA
                     AIFKRCYSNGLILMKAGEHNQVIRFLAPLNITPEQLDEGLDILESALAAESARA"
     gene            2294..3946
                     /gene="ggt"
                     /locus_tag="VIL39_10435"
     CDS             2294..3946
                     /gene="ggt"
                     /locus_tag="VIL39_10435"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5481888.1"
                     /translation="MMSTHKRPAIARNAMVVAAHPLAAAAGITMLGRGGNAVDAAVAT
                     LAALSVVEPFMSSIFGAGFMLIRLPSGETIALDNYAVAPGAARADMYEPLPPAGGLYN
                     TVASKNDIGHLAVAVPGALAAYAMAVQRYGRLPWATVLGPAIGLAAEGYPATPYLVDA
                     VRTEADALSRYSDSAKIFLPGGRLVEPGAIIRRQEYAETLRQVAAEGPDALYRGDLAR
                     TVVADMERSGGLITMEDLAAYQVLERTPIRGTYRGYEILAMPPASSGGIALVQMLNLL
                     EGFDVREAGFGSARSIHLLAEALKIAFADRRAYLGDPSAAGNPPTWLTDKRYAAMRRP
                     EIGANRAGAPSPGQADALEGNCTTHVSTADAEGYVVSTTQTLNHLFGSRVTVPGTGML
                     LNNCMRLFDPQPGFPNSVGRGKRMLSSMTPAMVFKDGKPFLAVGSPGGTRIIAAVLQV
                     IVNVIDHGMSLQEAVEAPRVHTGTENEGLLMEPGFAAETLSALVGMGHCVQPVDVVGG
                     GMQAVLFRDGQLEGSSCWRSDGVALGLSGGRTSIESGKALVY"
     gene            3962..>4251
                     /locus_tag="VIL39_10440"
     CDS             3962..>4251
                     /locus_tag="VIL39_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5481889.1"
                     /translation="MNIGSLLRPDLILFGGNLITIDPAQPRAQAIAIRGETILAVGAD
                     ADVLSLAGAGTRRTDLHGMTVVPGFNDAHNHMVFFGQQLKGVDLQTNPARSIS"
BASE COUNT          805 a         1312 c         1334 g          800 t
ORIGIN      
        1 tggcctgcat ggcccggggt ttcgtgggtg atgcctgcct gatccccgaa ccgtttgagc
       61 aaaaaatcct caccgccgag gtgggtgttc tctggatgcg agcggatgtc ttcggctctg
      121 ccggccacac ccgcgcggcc agcaccatgg tgaacgccat cgataaggca taccaactcg
      181 tcgccgccat gcgccaactg gagcagcagt ggaaccagga gcggcacccc gctttcgctg
      241 atcaaccgca cccgctgaac ttcaaccttg gcctgatcaa gggcggcaat tggccttcca
      301 gcgttccggc ccactgcgag ttttccatgc gtctctccat gtaccccggc gtcgaccccg
      361 agaaggcgaa gcggcaggtc caggcccata tcgaccagtt ctgcgccgac gacccatggc
      421 tgaaggcgaa tccaccggaa atcacctggt acggtcacca cgactggggt gtttatgaac
      481 cgaacccaga acgcaacccg ctcatgcagt cggtcgcgcg agcccatact gacgtgaccg
      541 gcacgcccgt tgaatatcat aattccaccg cctgcaccga cgtccgtttc tggacggagc
      601 attggcataa gccctccacc tgttacgggc cgattggcga gcagcttcac gccgccaatg
      661 agtgggtaag cctaaagagc gtcagcgaga caaccaaggt agtcgcagcc acggtcatcg
      721 actggtgcgg cgtagcccat taagtagaaa ggtagattca atggctaaga cagtgagcat
      781 cgtgaccccc gtccctggtc ccaaatcccg ggagctgctg gtccggcgtg aggcgatcgt
      841 gccgcgcggc gtcggcaatg tcgcccccat cttcgcagcc cgcgcccatg gtgccaccat
      901 cactgatgtg gacggcaaca cctttatcga tttcgccggc ggcataggcg tggtcaatgt
      961 cggccacacc caccctgagg tgaacgacgc catccatgcc caggtggata agttcctgca
     1021 cacctgcttc catgtgacca tgtatgaagg ctatgtccgg ctggccgagg agcttgcccg
     1081 cctcacaccc ggcgatttcc gcaagaagac gctgctcgtg aatagcggcg ccgaggcggt
     1141 cgagaatgcg gtcaaaattg cgcggaggtt caccggtcgc cctgcggtgg tcacgttcga
     1201 gaacgctttc catggtcgga ccctgctgac gatgtccctg accgccaagg ccagcacata
     1261 caagcacggc ttcggtccct ttgctccgga aatctaccgg gccccgttcc cgaacaccta
     1321 ccagtcaccc tttgcctcag aggctgaagc ggtggagtgg gccttcaccg gatttcgccg
     1381 tctggtcgaa acggagatcg gacaggacaa ggtggctgcc gtgatcctag agcccgtgca
     1441 gggagaatca ggcttcctgg taacgccgcc agccttcatg aaacagctcg tcgcgtactg
     1501 caacaagaac ggtattctct ttatcgccga tgagattcag acggctttcg gccgcaccgg
     1561 caagtggttc gctgtagagc attacggagt gacgccggat ctgttcacta tcgccaagtc
     1621 gatgggcgct ggcctgccga tcgcggctgt ggtcggccgt gccgaagtga tggacgctcc
     1681 acaggtgggt ggcctcggtg gcacctatgg tggcaacccg gtcgcgtgcg ccggcgccct
     1741 gaagacgatt gaggtgatgg ctcgggacgg ttacaacgaa aaaggcgcgg ccatcggcaa
     1801 aaaggtgcat gcccgcttcc gggatttcca gaaaaagtac gctttggtgg gcgacgttcg
     1861 gggactcggt gcgatggtcg ctatggagct ggtcatcgac cggaggacca aggaggcggc
     1921 cgctgagaag gctgcagcca tcttcaagcg ctgctatagc aacggcctaa ttttgatgaa
     1981 ggccggcgag cacaatcagg taatccgctt cctcgcacca ctcaacatta ctcctgaaca
     2041 gcttgacgag ggcttggaca tcctcgagtc ggccctggcg gctgagtcag cccgcgctta
     2101 ggagaacgga attgcaaggt caagatcgat ctcaattgcg agcgactgag gccctcgata
     2161 ccgtccatga cggatctcgg cgcaacagcg gaaaaagtcg ctgcgagacc gagtcaattc
     2221 actggaacga cgtggccgcc gccgaactgc ttttgggagt ggtacacacg ctcgccggga
     2281 aggtgcctct gaaatgatgt ctacacacaa gcgcccggcc atcgcgcgca atgccatggt
     2341 agtcgcagct caccctctcg cggcggccgc cgggatcacc atgctgggtc gaggtggcaa
     2401 tgcggttgat gccgctgttg ccaccttggc ggcgttgtcg gtggtggagc cgttcatgtc
     2461 gagcatcttc ggcgccgggt tcatgctgat ccggcttccg agtggcgaga ccattgcgct
     2521 cgataactac gctgtcgctc ccggcgctgc ccgggccgac atgtacgaac cgctgccgcc
     2581 ggcaggtggc ttgtacaaca ccgtggcgtc aaaaaatgac atcgggcacc ttgcggttgc
     2641 tgtgcccggc gctctggctg catacgccat ggccgtgcag cggtacgggc gcctcccctg
     2701 ggccaccgtg cttgggccgg cgattggcct tgcggccgaa ggctatccgg ccacccctta
     2761 tttggtggat gcggtcagaa ctgaggccga cgcactgtct cggtattcgg actccgccaa
     2821 gatttttttg cctggcggac gcttggtgga accgggtgcg atcatccggc gccaagagta
     2881 cgccgagacg ctgcgccaag tggcggccga gggaccggat gccctctacc gcggcgacct
     2941 cgcccgaacc gtggtggcgg atatggaacg cagtggaggc ctgatcacca tggaagactt
     3001 ggcagcctac caagtcctgg agcgaacccc cattcgcggc acctaccggg gctatgaaat
     3061 cctcgcaatg cccccggcaa gttccggggg cattgccctg gtccagatgc tcaaccttct
     3121 ggagggcttc gacgtccggg aggcgggctt cggctccgcc aggagcatcc acctcttggc
     3181 cgaggcgctg aagattgcct tcgccgatcg gcgtgcctac ctcggcgatc cgtctgccgc
     3241 gggcaatccg ccgacctggc ttaccgacaa gcgctacgcg gccatgcggc gaccggagat
     3301 cggtgcgaac cgggccggag ccccctcacc tgggcaagca gatgcgctgg aagggaactg
     3361 caccacacac gtgagcacgg ccgacgccga agggtacgta gtctccacca cccagacgct
     3421 caatcacctc ttcggctcgc gcgtgaccgt tccgggaacc gggatgcttt tgaacaattg
     3481 catgcgcctc ttcgatccgc agccgggatt tccgaattca gtgggacgcg gcaagaggat
     3541 gctcagcagc atgacgccgg ccatggtgtt caaggatgga aagccgttcc tggccgtcgg
     3601 gtcaccgggc ggcacacgta tcattgctgc cgtgctgcaa gtgatcgtca atgtcatcga
     3661 ccacgggatg agcctgcaag aggcggtcga agccccgcga gtccacaccg ggaccgagaa
     3721 cgagggtttg ctgatggaac ccggcttcgc ggccgagaca cttagcgccc tggttggcat
     3781 gggccattgt gtgcagccgg tggacgtggt ggggggcggt atgcaggcgg tactattccg
     3841 agacggccag cttgaaggtt cgtcctgctg gcgttcggac ggcgtggcgc tggggctgag
     3901 cggcggtaga acgtctatcg agtccgggaa ggctttggtg tattgacagg gagtatgaat
     3961 tttgaatatc ggatcgctgc tcaggccgga tcttattctt tttggcggca acttgattac
     4021 catcgaccct gcgcagccgc gagcccaggc aatcgccatt cggggcgaaa caattttggc
     4081 ggtcggcgcc gatgcggatg tgctgtcgct cgcgggcgcc ggtacgcggc gcaccgacct
     4141 ccacggcatg acggtggtgc ccgggttcaa cgacgcgcac aaccacatgg tctttttcgg
     4201 tcagcagctg aagggcgtcg atctgcagac caaccctgcc cgctcgatct c
//
LOCUS       DATCGS010000509         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_340599, whole genome shotgun sequence.
ACCESSION   DATCGS010000509 DATCGS010000000
VERSION     DATCGS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340599"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIL39_10445"
     CDS             complement(<1..1041)
                     /locus_tag="VIL39_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY5481890.1"
                     /translation="MKRILLTFTLVGILATCLAGFAQTNSPAADAAASSAPVVAAPAP
                     APEAATPSSIAQPDAIIPLIVMDDVPLTDAIRNLARQSGLNYMLDPKIAFGQVGPDGK
                     ATPQPSVSIRWENITAEQALAALLNNYTLQLVEDPKSRIARISTKDPAALPPLSTEII
                     QLKYASPSNVVANIQSILSDKRSKVMPDNRTSQLIVVATESEMVDVLKMIERLDTKTK
                     QVLIEARLLETLVNPTTAKGVDWSGTLAKQHVSYGNGVTSGNIGQTSDNSSSGNTSGN
                     KSKNSAQTSLTAAPGTQVTETLPGGRIITYTPDSSKSSTDSGNSSSSGSSAASSLASS
                     GVSSILNSVIGNG"
     gene            1598..3763
                     /locus_tag="VIL39_10450"
     CDS             1598..3763
                     /locus_tag="VIL39_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481891.1"
                     /translation="MAGFRRFVLALWLPAFCGAALMSQAVLQFDVFLGYDWIVPQASW
                     FPVVCEIKNDGPPFVGTVELDGGRYNPGQTRRAVVELPTGTLKRFVIPVFATAGTLGA
                     WDVRLLDERGKVRGEQTGLRAKRQIASIAPLVGALARTPAGMPVIRPILPQDTALQPA
                     AARFLPAVFPDNPVVLEGLDCLYLNSEKALDLKVNQVNALFAWLNGGGHLIVGIEQIS
                     DITATPWLRNVLPCDLNDIRTVPGHPELQEWLRSATWSTNLAGLSPRQSGSSGSARSA
                     PRPNASAADPFGDLPDDPAFEGAAMQVAMGAVRDGRVVVAAGDKPLIVTANRGRGRVT
                     VLLFSPEREPFRSWKNLPTFWAKLAEVPGIWYVSADYRPQGGWSSDGIFGAMIDTRQV
                     HKLPVTWLLLLLIVYLVVIGPLDQRWLKRIGRPMLTWITFPCYVVLFSLLIYFIGYKL
                     RAGESEWNELHLVDVLLKGEQAELRGQTYASIYSPSNQRYRLEGRQEFATLRSEFAGT
                     WSGAQPGEKANILQEGDGSFKAEIFVPVWTSQLFVSDWWQPAPVPLSVSVVPQGEQWE
                     VKVENRTEHSLANAHVVIEDYIMNLGEIPAREARTFTVSKAKGTRLNDFVGRYGSTFQ
                     SAVRGRQRTFGGSESGRVDDLANGTMAASFLSRLSGGQDPMHHFVSPPGLDLSPVVEH
                     GNAVLLAWAGDYSPVKPLRQFTPRRSQKNTLWRVAVPVK"
     gene            3780..>4012
                     /locus_tag="VIL39_10455"
     CDS             3780..>4012
                     /locus_tag="VIL39_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5481892.1"
                     /translation="MNAPPIPAVQTFGLTRLYGNITALDKLDLTVNQGDLFGFIGSNG
                     AGKTTTLRILATFLAPSSGSAQILGRDLLKEADA"
BASE COUNT          696 a         1112 c         1380 g          824 t
ORIGIN      
        1 gccgttgccg atcacggagt tcagaatgct gctgaccccg gaactggcca gactgctggc
       61 cgctgagctg cccgaagaac tgctgttccc tgagtcagtt gagcttttac ttgaatcggg
      121 tgtgtacgtg ataatgcgtc cgccgggcaa cgtctcagtc acctgtgttc cgggcgcggc
      181 ggtcaaggag gtctgggccg agttcttgct tttgttcccg ctggtgttcc cgctggagct
      241 attgtcggag gtctggccga tgttgccgga cgttacgcca ttgccgtagc tgacgtgctg
      301 cttggcgaga gtcccggacc agtcaacgcc ttttgcggta gtcgggttca cgagcgtctc
      361 cagcaagcgc gcttcaatga gcacttgctt ggtcttggta tcgaggcgct cgatcatttt
      421 aagcacgtcc accatttccg attcggtggc gacgacgatc aactggctgg tgcggttgtc
      481 gggcatgact ttgctgcgct tgtccgagag gatggattgg atattggcga cgacgttgga
      541 ggggctggcg tacttgagct ggataatctc ggtgctgagc gggggcagcg cggccggatc
      601 tttggtgctg atgcgggcga ttctgctctt ggggtcttcc actaattgga gggtgtagtt
      661 gttgaggagg gcggcgagcg cctgctcggc ggtgatgttc tcccagcgaa tggacacgga
      721 gggctggggc gtcgctttgc cgtctggccc gacctgaccg aaggcgatct tggggtcaag
      781 catgtagttc agcccggact ggcgcgcgag gttccgaatc gcgtcggtga gcggcacgtc
      841 atccatgacg atgagcggga tgatggcgtc cggttgggcg atgctgctgg gtgtggccgc
      901 ttcgggggct ggggccgggg ccgcgaccac gggagcactg ctggccgcag cgtcagccgc
      961 aggagagttg gtttgggcaa agccagccag gcaggtggcg aggatcccaa caagcgtaaa
     1021 ggtgaggagt atgcgtttca tgtgtttaaa atggctctga cttgcaccgg actgcgaccg
     1081 actgctattt catttaaggg cctttaagca gtcgcggtgc caacggcata agcacacgta
     1141 gtttccgccc tgagcgcagg aaagtggcca ctcggcaaaa aagctgtcca tcattaagac
     1201 aaaccggtgg actggcgggg gcggcgctaa ggtgattcaa tcccttcggg attggcgcac
     1261 ctcaagcagc cgaagcagtt tttaggccgc ctgatcgcca ggcaggccgc tggtgaggtc
     1321 tggacctcta tttgagccgg ggtgagcgga tgaaagacta tttctacccc gccatgcgag
     1381 gggtacaaga aatgagaggc tcccggcagg acgctcgccc tcccgaagtg ttgcgtgacg
     1441 atggacgaag cggcttgtct aactgcttgg aaataacatg acagtgaatt gaacgttgcc
     1501 aaaatggcag ggtctgctta tagagtaggc agttcgaact tgtcgcgagg aacacatggc
     1561 agggcattct gaatccagac tcaggtcaca accgtgggtg gccgggtttc gtcgttttgt
     1621 gctggcgttg tggttgcccg ccttttgtgg cgccgcgctc atgtcgcagg ccgtgctcca
     1681 attcgatgtg ttcctcggct acgactggat cgtgccgcag gcgagctggt tcccggtggt
     1741 gtgcgagatc aagaatgacg gaccgccgtt cgtgggcacg gtggaactgg acggaggccg
     1801 gtacaacccg ggacagacgc ggcgcgcggt ggtggagctg ccgacgggga cgctcaagcg
     1861 gttcgtgatt ccggtgtttg cgaccgcggg cactcttggc gcctgggacg tgcggttgct
     1921 ggatgagcgc gggaaggtgc ggggcgagca aacgggtctg cgggcgaagc ggcagatcgc
     1981 ctccatcgcc cccctggtcg gcgcgctggc gcggacgccg gcgggcatgc cggtcatccg
     2041 gccaattcta ccccaggaca ctgcattgca gccagcggcg gcgcggttcc tgccggcggt
     2101 ttttccagac aacccggtgg tgctcgaggg gttggattgt ctttacctga attcagagaa
     2161 ggccctggat ctcaaggtca accaggtcaa cgccttgttt gcctggctca atggcggcgg
     2221 tcacctgatc gtgggcatcg agcagatttc cgacatcacc gcgacgccgt ggttgcgcaa
     2281 cgtgctgccc tgcgacctga atgacatccg gacggtgccg gggcatccgg aacttcaaga
     2341 gtggctgcgg agcgcgacct ggtccactaa tctggccggg ctgtcgccga ggcaatcggg
     2401 gtcgtccggg tcggctagat ctgcaccgcg accgaacgcg agcgccgccg atcctttcgg
     2461 cgacctgccg gatgatccgg cctttgaagg ggcggccatg caggttgcca tgggcgcagt
     2521 tcgggacggg cgggtggtgg tggctgcggg tgacaagccg ttgatcgtca cggcgaaccg
     2581 tggacgcggg cgggtgacgg tgctgctgtt cagcccggag cgcgagcctt tccggtcgtg
     2641 gaagaacctg ccgaccttct gggcgaagct ggcggaggtg cccggcatct ggtacgtctc
     2701 cgccgattac cgcccgcaag gcggctggag cagcgatggc atcttcggag cgatgattga
     2761 cacgcggcag gtccacaagc tgccggtcac ctggctgttg ctgctgttga ttgtgtatct
     2821 ggtcgtcatt ggcccgctgg accagcgctg gctgaagcgg attggccggc cgatgctgac
     2881 gtggattacg tttccctgtt atgtggtgct gttctcgctg ctcatttatt tcatcggcta
     2941 caagttgcgc gccggggagt cggaatggaa cgaactgcac ctggtggatg tgctgctcaa
     3001 aggtgagcaa gccgaactgc gcgggcaaac ctacgcatcg atctattccc cgtcgaacca
     3061 acgctaccgg ctggagggtc ggcaggagtt cgccacgttg cggagcgagt ttgccggcac
     3121 ctggagcggt gcgcaaccgg gcgagaaggc gaacatcctg caagagggtg acggttcgtt
     3181 caaggcggag atcttcgtgc ccgtctggac cagccagctt tttgtgagcg attggtggca
     3241 gccggcgccg gtgccgctca gcgtcagcgt ggttccccaa ggcgagcagt gggaagtaaa
     3301 ggtcgaaaac cggacggagc acagcctggc caacgcgcac gtggtcatcg aggattacat
     3361 catgaacctg ggcgagatcc cggcgcggga ggccagaacg ttcacggtct caaaggcaaa
     3421 aggcacgcgc ttgaacgact ttgtcggccg atacggctcg acgtttcaat ccgccgtgcg
     3481 gggacggcaa aggaccttcg gtggaagtga atccgggcga gttgatgatc tggccaacgg
     3541 cacgatggcc gcgtccttcc tttcgcgcct gagcggtggc caggatccga tgcaccactt
     3601 cgtctcgccc cctggattgg atttgtcccc cgtggttgag cacggcaacg cggtgctgct
     3661 ggcctgggcg ggggactact cgccagtaaa accgctgcgc cagttcaccc cgcggcggag
     3721 ccagaagaac acgctgtggc gcgtcgccgt gccggtcaaa taagtctaaa gtctatagta
     3781 tgaacgcccc cccgatcccc gccgttcaga cgttcggttt gacgcgcctc tacggcaaca
     3841 tcaccgcgct ggataagctg gacctcacgg tcaaccaagg cgacttgttc gggttcatcg
     3901 gctcgaacgg cgcgggcaag accactacgc tgcgcattct ggcgacgttt ctggcgccgt
     3961 cgtcgggctc cgcccagatt cttggtcgcg acctgcttaa ggaggccgat gc
//
LOCUS       DATCGS010000510         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_637215, whole genome shotgun sequence.
ACCESSION   DATCGS010000510 DATCGS010000000
VERSION     DATCGS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637215"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VIL39_10460"
     CDS             <1..721
                     /locus_tag="VIL39_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5481893.1"
                     /translation="QVSTYESPWHGPNVPRWDKDLDDIVRWLEALPQPLALMTCNDVR
                     GLHVLDACARLGALVPEQVAVVGVDNEEILCELCTPPLSSVEPAPERIGYEAAELLDC
                     LMTGSSPRSKHISVEPMRVVERRSTQALAIEDRVVAAAMRFIREQAVFGCSVSDVLKP
                     LRVSRSLLERRFRQHLKRSPQAEIRAVQLGRVRQLLTETDFTLEHIAELSGFEHPEYM
                     SVVFKRTCGLTPGQYRRQHPR"
     gene            718..981
                     /locus_tag="VIL39_10465"
     CDS             718..981
                     /locus_tag="VIL39_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481894.1"
                     /translation="MTFIALAGRIPLWTLAEPAIKRYSYIPFGETGNAAGEETISRKI
                     NIMGDKSPKSTNKQASQKQAKASGAKVKKQQAIVAKQVAGKKK"
     gene            complement(1437..>2488)
                     /locus_tag="VIL39_10470"
     CDS             complement(1437..>2488)
                     /locus_tag="VIL39_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481895.1"
                     /translation="RHLTGMFYAPHEVPTDRIAFLHENEDPAPVSMEKTTGPTEDAVL
                     RVVVDECVGLDSLLARHFQRRLRPDQAVEFTLLAKAHRAIPDSEILGKLLGPGTVLLT
                     SDRVLHNQACDLGLRSYTLNALGKLIQEKLPDVRAPRAVPARGGDVLKTDYTHGPNVI
                     ASALRAGLDERALKKYRTRRRRIRSYFGSESNISQVALTVGARVARQGCISGFFLAVA
                     GYSGVKGIRASEGYALGMGGDWDPARCLIHALQELYLLQLEAVCVDLFIIPRDSLDLC
                     RQLQSKPATACDRPSARAFSKVLHALASARVFPCVKGPVFDSMEHKLNQLARTPNNEL
                     VPVDFGRIIESLNHC"
BASE COUNT          526 a          750 c          747 g          465 t
ORIGIN      
        1 ccaggtttcc acctatgaat ctccgtggca tggcccgaac gtgccgcgct gggacaaaga
       61 cctcgacgac atcgtgcgct ggcttgaagc cttgccccag cccctcgcgc taatgacctg
      121 caacgacgtg cgcggtctgc atgtgctcga cgcctgcgcc cgcctgggcg cattggtgcc
      181 ggagcaagtg gccgtggttg gcgtggacaa cgaggaaatc ctctgcgaac tatgcacccc
      241 accgctttcg agtgtggagc ccgccccgga acgcatcggc tacgaagccg cggagttgct
      301 cgactgtctc atgacgggga gttcgccgag aagtaaacac atttccgttg agccgatgcg
      361 cgtggtggag cggcgttcga cgcaagcgct ggccatcgaa gatcgcgtgg tcgctgcagc
      421 catgcgcttc atccgcgaac aagccgtctt cggctgtagc gtgagtgacg tgctcaagcc
      481 gctgcgcgtg tcccgcagct tattggaacg tcgatttcgg cagcatctga aacgctcgcc
      541 acaagcggag attcgcgccg tccaattggg ccgcgtgagg caattgctga ccgaaaccga
      601 cttcaccttg gaacatattg ccgaactctc cggcttcgag cacccggaat acatgagcgt
      661 cgtgttcaaa cggacgtgcg gcctcacgcc cgggcagtat cgccggcaac atcccagatg
      721 accttcatcg ctttagccgg aagaatcccc ctttggacgc ttgcagaacc tgccataaag
      781 aggtatagtt atattccctt cggagaaact ggaaatgccg caggtgagga aaccattagt
      841 cgaaagataa acatcatggg cgacaagtcc ccgaaatcaa cgaacaaaca ggcatcacaa
      901 aaacaggcca aggccagcgg tgccaaggtg aagaagcagc aagcgatcgt cgcaaagcaa
      961 gtagccggta aaaagaagta agtcccggcg cgctcgcctc tccaggccgg caacgagacg
     1021 acgaagcccc gtgccggcat gaggtgattc taccgcaggc actgacgggc gggattgccg
     1081 cttgaagacg ctggttcttc tcacccgcag cccttacgtg agtaacacgg ctgttaccct
     1141 gactggaggc aagctggtgc gccacagctt cccgctcggc agcgtgcgcc tgttggaaac
     1201 catgccttgc agcgacccgc ccaccccggc tgaactcgcc gcctgccgcc aatggctgaa
     1261 ggattttctg cggaaggaag tgcgaccgaa gctgatagag gaaatgggag gcggcgttga
     1321 tggaggtgct ctctggctgc gtgctgcgag gctccaccag gatcggggcg tccatcgtcc
     1381 cccgccacaa tccagagcgg gcgcactttt cccccgtcgc actcaatttg ctttcttcag
     1441 cagtggttga gggactcgat gatgcggcca aaatcaaccg gcacgagctc gttgttggga
     1501 gtgcgggcga gttgattcaa cttatgctcc atgctatcga agaccggccc tttcacgcag
     1561 ggaaacacgc gggctgaggc gagcgcatgg agcactttgc tgaaagccct ggccgaaggg
     1621 cgatcgcagg cagtggccgg cttggactgt agttgccggc acaagtccaa cgaatcacgc
     1681 ggaatgatga acaaatctac acagaccgcc tctagttgca gcagatacag ttcctgcaag
     1741 gcgtggatca aacaccgagc ggggtcccag tctcctccca tgccaagggc gtatccctcg
     1801 ctcgcgcgaa tccccttcac gccagagtag cccgccaccg cgagaaaaaa gccgctgatg
     1861 cacccttggc gcgccactct ggcgccgacc gtaagagcga cctggctgat atttgactcc
     1921 gagccaaagt agctgcgaat gcggcggcgg cgggtgcgat acttcttgag ggcgcgctcg
     1981 tccaaccccg ctcttagcgc gctggcaatc acgttgggtc catgggtgta atccgtcttg
     2041 agcacatccc cgcctctggc ggggacggcc cttggggcac gaacatccgg cagcttctct
     2101 tggattagct ttccaagcgc attcagggtg taggagcgca gcccaaggtc gcaggcttga
     2161 ttgtgaagca cccggtcaga ggtgagcaga acggttccag gcccgagcag cttgcccagg
     2221 atctcggagt ccggaatggc gcggtgagct ttggcaagta aagtgaactc gacggcctgg
     2281 tctggccgca agcgccgctg aaaatgccgg gccaggagtg agtcgaggcc aacacactcg
     2341 tccactacca cccggagcac tgcgtcttcg gttggccctg ttgttttttc catgctcacc
     2401 ggagcaggat cctcgttttc atgtagaaat gcaatccggt cggtcggcac ctcgtgcgga
     2461 gcataaaaca tcccggttag gtggcgat
//
LOCUS       DATCGS010000511         6202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_290308, whole genome shotgun sequence.
ACCESSION   DATCGS010000511 DATCGS010000000
VERSION     DATCGS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290308"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(843..965)
                     /locus_tag="VIL39_10475"
     CDS             complement(843..965)
                     /locus_tag="VIL39_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481896.1"
                     /translation="MYERIPYSWTLSFRGFNVHVGEPVPPEAKADLEALAQYIT"
     gene            complement(1316..2512)
                     /locus_tag="VIL39_10480"
     CDS             complement(1316..2512)
                     /locus_tag="VIL39_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5481897.1"
                     /translation="MNALKRTCLTGMLTAALAVMLCGCAHLNSAAPQPGANSSDPVSR
                     AIQGVKDQYAPNNRLAIFNVGAQRRGGNLVLTGEVDRAEAKVETVRAVERTGVRVTDH
                     IKVLPDESLGDQVWGISCLSVASGREQPEHKAEMGTQVLMGEVVRVWKPSTNAVFRWY
                     LTQSGDGYVSWLQKGTFVRCTREQVETWNRGPLLVVTAFEDLILEQPQADAQPVSDVV
                     LCDLVRKIGEEGDWCKVELPDQRTGFLPRKAATDYHAWKQARQPTAKNIERTARRFIG
                     RPYLWGGNSPKGFDCSGFSKTVFYLNGIDLMRDSSQQARQGVAVPLDGDLSQLKKGDL
                     LFFGHRARRGKPERVVHVGIYLGNKLFIHSSERVHISSLDPNSPIRDENRIQTLLRAR
                     RVLPGE"
     gene            2843..5179
                     /locus_tag="VIL39_10485"
     CDS             2843..5179
                     /locus_tag="VIL39_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481898.1"
                     /translation="MAVALALPGCVFLWFASWFRLAILVPLLVVILGWQVSLQVSAAP
                     ATNTGVKIAAPGFAGSQSCRECHEKFYQLWSTSFHGLAMQPYTSELARTNLTEQKAEI
                     VAGKYRFLADIRKSVVLERTAEGEKRYPIVQVMGGKNVFYFLTPLERGWLQVLPVAYD
                     VRRREWFDTTASAMRHFGDRRDEALYWKDRPLTFNTSCFGCHVSQLSKNYELKSDSYH
                     TTWAEPGINCETCHGPSGGHVSLFRALPTNQPAPADIKLIVLSKLTTEQRNATCAPCH
                     AKMSPLTMNFAPGDRYFDHFDLVGFESPDFYPDGRDLGENYTYTQWRVSPCAKSGQLD
                     CVHCHTSSGRYRFKDPVKANDACLPCHAARVANAPEHTHHKAGTAGNECISCHMPMTE
                     FARMRRSDHSMRPPTPAATLLYNSPNACNLCHTNQNAAWADKFVREWRPRDYQKPALE
                     RAALIAAARKQDWKKLPEILAYLSLTNREEVQTTSLVRLLAGCPADEQWPVLRSLMAD
                     PSPLVRASAAEALGQRLDQPNTAALCKATGDDYRLVRVRAATALAAVPEESLPEGQRA
                     RVRSALGELMESMRSRPDDMASHYNLGNFHMSRSQMPEAVAEFETACRLQPDALPPRV
                     NVALAYNALGQNDKAEASLRQALSLVPTNSAANLNLGMLLAEMGKMSEAEQAFRAAFK
                     ADPKSAQAAYNLGVLLSKEHPKEALTWCGRAAELRPENPQYGYTYAFYLYQAGQLDEA
                     LKAIRSVLQRHPAHEDSRLLERQLLQEQERKRSGGQGR"
     gene            5773..>6202
                     /locus_tag="VIL39_10490"
     CDS             5773..>6202
                     /locus_tag="VIL39_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481899.1"
                     /translation="MNRLPSTDYKNQLPGNATSRAFTLIELLVVIAIIAILAALLLPA
                     LAKAKAKAQRVYCISNLKQVALACKLYADDNGSKIVSAYPTFGGFTNGWCAGNAEADG
                     SAGLYKYGGSDPAGIQAGALWPYTKALGIYHCPADHRIATA"
BASE COUNT         1152 a         1989 c         1832 g         1229 t
ORIGIN      
        1 ccgtttctgg ccatttctta tccaggcgtc tggaacggcg gctgtaccca caggcccttg
       61 gctcagcacg gtttatcatg tccgcgcctg caaacgctac tacggcctga tccgccagtc
      121 tgacgaactc cgtccagctt ggctttcgca gcttacgctg gccggtcttt gcccgtgtcg
      181 ggcggttcgc ctcaccttcc cttctttggc tgtcgtttac ggcagagctt gccccagccg
      241 gggtctcccc caaccggagt ctgctatcac tactcggccc aaccgtccct tgccgaggca
      301 ggacttttac ctgcaagcat gtcaaaggtt gaaggctgca cataggaatc tgctttgcgc
      361 gcccactcat actgatcgct gcgcgggtga gggagaattg atttccgagc ttttaatcac
      421 aacgggaccg agtctatcag ggggaaagta tctatgcaag gtgctttttg tctattttag
      481 gctctcggcg cgagccctca ggcaaaaact gcaagatacg tgagcagagg attctctcgg
      541 cgcttgcttg gccggctccc gacaaaagcc cacttccgaa ccggcttgcc agaggacggt
      601 tttcaatcgg ttatcacatt ctcgtagtcg ggcattggat cttcgttcgt gcagggctcg
      661 taggtgtata gtgaccgtgc tcccgatgat tcgctcaagc tccgtcggag cggcatggcc
      721 ctcgccggac ctcaccatgc cgccccgatg gggcttaaga cggttctgtt tggacgctgg
      781 tactataaac atgtcgctcc tacggagctg gccgcttagc tcccggcggt ccccacgatc
      841 ctttacgtga tgtactgggc cagggcttcc aggtcggcct tggcctctgg tggcaccggc
      901 tcgccaacgt ggacgttgaa accacgaaat gaaagggtcc aggaataagg aatgcgttca
      961 tacatccgcc tcgccgtgcg cccgcttgtt gacgctgcgc gggtgaatcc cccgggatac
     1021 tcgatgcgaa gccttcgagg tgcgcccgga taccagacta tgctcaaacc actgtgaatt
     1081 ccaaaatgac ccaagctatg ggctgacttc tgctacgttc taaaccgggc ttggtatcag
     1141 cgagcttatc gagccgctga tcctctggca acttagcttt atgacaacca agaccgtcga
     1201 gcttgaaatg gacgccgttg cgaaactgga agcggcgaga tggagcgccg gggaatctct
     1261 ttcggcggta gtgcgtcgcg ccacatttac cggcaagccc cacctcgccc gcgagttact
     1321 cgccagggag gacgcgccgg gctcgcagca gggtctggat gcggttttcg tcgcgaatgg
     1381 gtgagttggg atcgaggctg ctgatgtgca cccgttcgga ggagtggatg aacagcttgt
     1441 tcccgagata aatgcccacg tgaaccaccc gttccggctt gccacgccgg gcgcggtgcc
     1501 cgaagaacag caagtcgccc ttcttgagct ggctcaggtc gccgtccagc ggcaccgcca
     1561 ctccctgccg cgcttgctgg ctggaatcgc gcatcaggtc tatgccgttg aggtagaaca
     1621 ccgtcttgct aaacccggag cagtcaaagc ctttgggcga gttgccgccc cacaggtagg
     1681 gccggccgat gaatctccgg gccgtgcgct cgatgttctt ggcggtgggc tgccgcgctt
     1741 gtttccatgc gtgataatcg gtggccgctt tcctggggag aaagcccgtc cgctgatccg
     1801 gcagttccac cttgcaccag tcgccttcct cgccgatctt ccggaccagg tcgcacagga
     1861 cgacatcaga caccggctgc gcgtcggcct ggggttgctc cagtatgagg tcctcaaagg
     1921 cggtcacgac caatagcggt ccacggttcc acgtctcgac ctgctcgcgc gtgcaacgga
     1981 cgaaggtgcc cttctgcagc cacgagacgt agccatcgcc cgactgggtc aagtaccacc
     2041 taaacacggc gttagtggac ggtttccaca cgcgcacgac ctcgcccatc aacacctgcg
     2101 tgcccatttc cgccttgtgc tccggctgct cccggccgct ggcgacgctg aggcaggaga
     2161 tgccccacac ctggtcgccc agactctcgt ccggcagcac cttgatgtgg tcggtcaccc
     2221 tgaccccggt ccgttcgacc gcgcggacgg tttcaacctt ggcctcggcc cggtccacct
     2281 cgccggtcaa gacgagatta ccccctctgc gctgggcgcc cacattgaaa atggccaggc
     2341 ggttgttggg cgcgtattgg tctttaacgc cctgaatggc gcggctgacg gggtcggagc
     2401 tgttggcgcc cggctggggg gcggcgctat tcagatgcgc gcagccgcac agcattaccg
     2461 cgagtgccgc ggtcagcatc ccggtgagac aggttcgttt cagtgcgttc acagatttag
     2521 actatcggtt acaggcaaac gggatttgga tccaaaagcg agcagaattg gaagcccagg
     2581 ctttgtgcgg gcagggcggc tgacaaagtc gccgggatgc cccggcacca cggatgtgga
     2641 gtgcggcggc agtgcccaga agcgccaccc ttgtgattgt ggtggacgcc gctccgagtg
     2701 gtgggcaatg ctttggggga ttgcgatgaa tcctggcttc tggcttgtta acgcagagac
     2761 gcagagagcc gcagagattc gcagagcgct cttgttcttt gcgtttctcc gcgatgctct
     2821 gcgtctctgc gttaaccctg gtatggctgt ggctctggca ttgccgggct gcgtctttct
     2881 gtggttcgcg tcgtggttcc ggctggccat cctggtcccc ctgctggttg tgattcttgg
     2941 gtggcaagtc tcgctgcaag tcagcgcggc tcctgccacc aatacggggg tcaagattgc
     3001 agccccgggc tttgccggct ctcagagctg ccgggaatgc cacgagaagt tctaccagtt
     3061 gtggtccacc tccttccacg gcctggccat gcaaccctac acgtccgagt tggctcggac
     3121 caacctgacc gaacagaagg cagagatcgt cgcgggcaaa tatcggttcc tcgccgacat
     3181 tcgcaaatcg gtcgttctcg aacgcactgc cgagggcgag aaacgctacc cgattgtgca
     3241 ggtgatgggc ggcaagaacg tgttctactt cctgaccccg ctcgagcgcg gctggctcca
     3301 ggtgctgccg gtggcttatg atgtccggcg ccgggaatgg tttgatacca ccgccagcgc
     3361 gatgcgccat ttcggcgacc gccgcgacga ggcgctttac tggaaggatc gaccgctgac
     3421 gttcaacacc tcgtgcttcg gttgtcacgt cagccagctt tccaagaact acgagctgaa
     3481 gtccgacagc taccacacca cctgggcgga gccggggatt aactgcgaga cctgtcacgg
     3541 cccatcggga gggcacgtga gcctgttccg cgcgctgccc accaatcagc ccgcccccgc
     3601 cgacatcaag ctcattgtgc tcagcaaact caccaccgag cagcgcaacg cgacctgcgc
     3661 tccctgtcat gccaagatgt cgccgctaac catgaacttc gctccgggcg accgctactt
     3721 cgatcatttc gatcttgttg gctttgagag ccccgacttc tatcccgatg gccgcgacct
     3781 gggggagaac tacacctaca cccaatggcg ggtcagcccc tgcgccaagt ccggccaact
     3841 ggactgtgtc cactgccaca cgtcgagcgg gcgctaccgg tttaaagacc ccgtgaaggc
     3901 caatgacgcc tgcctgccct gtcacgcggc gagagtggcg aacgcccccg aacacacgca
     3961 ccacaaggct ggcaccgccg gcaacgagtg catctcgtgc cacatgccga tgaccgaatt
     4021 tgcccgcatg cggcgcagcg accactcgat gcgcccgccc accccggcgg ccacgctgct
     4081 ttacaattcc cccaacgcct gcaacctctg ccacaccaac cagaacgccg cctgggctga
     4141 caagttcgtg cgcgaatggc ggccacgcga ttaccaaaag cccgcgctgg agcgcgccgc
     4201 gctcatcgcc gcagctcgga agcaggactg gaagaagctc ccggagatac tggcgtatct
     4261 ctccctcact aaccgtgagg aagtccagac cacctccctg gtccgcctgc tagccggttg
     4321 ccccgccgac gagcaatggc cggtgctgag aagcctgatg gctgatcctt cgccgctggt
     4381 gcgcgccagc gccgccgagg ccctgggtca acgcctggat cagcccaaca ccgccgcgct
     4441 gtgcaaggcg accggcgacg attaccgact cgtccgcgtg cgcgccgcca cggcgttggc
     4501 cgcagtgccg gaagaaagcc tgcccgaggg ccagcgtgcc cgggtgcgta gcgcgctcgg
     4561 cgagctgatg gagtccatgc gctcccggcc ggatgacatg gcttcgcact acaacctggg
     4621 caacttccac atgtcgcgca gccagatgcc cgaagcggtg gcggagtttg agacggcgtg
     4681 ccgcttgcag cccgacgcgc tgccgccccg cgtgaacgtc gccctcgcct acaacgcgct
     4741 cggccagaat gacaaggcgg aagccagctt gcgccaggcc ctcagtctcg ttcccaccaa
     4801 ctctgccgcc aacctgaacc tcggcatgtt gctcgccgag atgggcaaga tgtccgaggc
     4861 cgaacaagcg ttccgcgccg cattcaaggc tgaccccaag tccgcccagg ccgcctacaa
     4921 ccttggcgtg ctgctgtcga aggaacatcc caaggaggcg ctcacttggt gcggtcgggc
     4981 cgccgagctg cgcccggaga acccgcaata cggctacaca tacgcctttt acttgtatca
     5041 ggcgggccag ctcgatgaag cgcttaaggc gatccggtct gtgcttcagc gccaccccgc
     5101 gcatgaagac agccggttgc tcgaacgcca actcctgcaa gagcaggaac gcaagcgcag
     5161 tggcggccag ggccggtgag gtccgcttgc ggccgacctg ggggcaggaa agagaggttc
     5221 attcgggagg tctgtgcctg gcatagccgc ccactttggt ggtggtgtca ggatgcgccg
     5281 ttatgcggaa ctccgcataa cggcgcatcc agataccacc gccggatcgc aggactcggg
     5341 ttttccggcg gcaagactca gacgcaacaa ctcgatgcaa ccacagatgg gcgcagatca
     5401 acacagatag ctgcgtttgc aatactcggc aggcctgcca gcgccaaacc gttaccccat
     5461 tcctgcctgg gaaaactcat ggttcgactg gtttccgttt tgtcagcgct cagcctgcaa
     5521 ccttgcttgc tgcccattcc gccactgcca gaagaggatg ctgtagttaa gctcattttg
     5581 ccccaaaaca tgccccaaaa gcccaatgat tttcgcgggc gacggcctta actaagcgcc
     5641 attcgcctaa aggcggcgtt ccgggggtag tgcgcggatc gcgccctgcc ggaaagaagt
     5701 tgctcagtca ggcttgacct ggagttcggt tatgatatat cgtctaacta taagcgtaac
     5761 acagattgtt ttatgaaccg cctcccctca accgactata agaatcagct acccggaaac
     5821 gccactagtc gcgctttcac gctgattgaa ctgctcgtcg tcatcgccat catcgccatc
     5881 ctggccgccc tgctgctgcc ggcgctggcc aaggcgaaag ccaaagcgca aagagtttac
     5941 tgcatcagca acctgaagca ggttgcgctg gcctgtaaat tgtatgccga tgataatggc
     6001 agcaagatcg tttcggctta tcctaccttc ggcggcttca ccaacggctg gtgcgcaggc
     6061 aatgcggagg ccgacggtag cgctggtttg tacaagtatg gggggtctga cccggcgggc
     6121 attcaggcag gggctctctg gccctatacc aaggcgctcg gtatctatca ttgtcccgcc
     6181 gatcatcgta tcgcgaccgc tg
//
LOCUS       DATCGS010000512         7572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_193280, whole genome shotgun sequence.
ACCESSION   DATCGS010000512 DATCGS010000000
VERSION     DATCGS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193280"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VIL39_10495"
     CDS             complement(<1..1010)
                     /locus_tag="VIL39_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY5481900.1"
                     /translation="MNRKQFIILLVLVVVLGVAGLVLYQRNQSSWQGGGRQGATQKLL
                     GDLPVNDVASIVIKGGTNQLDLIKKDNLWRVKERNDYPASFSEISGFLLKAADLKATQ
                     TEEVGASQLGRYKLLPPGTGTNTAVLVELRDQGGKVIKSLLLGKTHMRKSEGRPSPMG
                     EMGDNEGFPDGRYVMVGTGAKTVAVVSDPLSNLEAKPDLWLSKDFFKVEKIRSIAVAY
                     PVATNSWKVTRETETASDWKLAEAKPGEQLDSSKTSSFSYALNAPSFADVLPADTKPE
                     QTGLDKPTVITVDTFDNFTYLIKVGQKTNDNLPMVVAVTAQIAKERTPGKDEKAEDKA
                     RL"
     gene            complement(1007..2941)
                     /locus_tag="VIL39_10500"
     CDS             complement(1007..2941)
                     /locus_tag="VIL39_10500"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY5481901.1"
                     /translation="MQKKSFQTILYSTVGVLAMLGILILFNFIAGAARTRLDLTQEKA
                     FTLSAGTKAILKKLDTPVTIRFYCTQSESATPETVFLKGYARKVEDLLTEYRQVAGSK
                     VKIEKYDPQPDSDAEDSARLDGIEPQSLAGADRIYLGLAVKCADEVQAIPFLAPNRER
                     LLEYDLTRAIVRAETPEKPTIGLMSPLPVFGMPSNPMMQQMGQPPGGSQPWTLVNELK
                     NDFNVKRVGMDVDKIDDDIKLLLVIAPKDITDKAQYAIDQFIMRGGKLVAFLDAQCLA
                     DNRQQNQMMANMGGGGSSLDKLPKAWGIQFDTSKVVADLKYKLQLRGRGGEPQDAPAW
                     LALTADAIDKDDVATAQLDNIWLPLSGAFTGTPVAGLKEAVLLRSSKDSQLVDAMLAN
                     IAGDNIMKEFKPSGVNYNLAIRLTGKFKTAFPDGAPQDKKDDKSDGEKKDEKKPEDKK
                     PGDSLKETKSDNAVVLFGDADMLYDPFTMRRIDSPFGAMQMAMNANLNLAQNIVEQMT
                     GDSNLIAVRSRASLIRPFIRVKEMEMAANEKFQSEIKRLEESAAESQRKINEMQAQKK
                     DKDQRFILSPEQRTELEKLRKEEAEARKHLKQVQKDLRKEVVSLQTRLKWINILAVPL
                     AVTVTGIVIAIVNRRKTSAK"
     gene            complement(2959..3705)
                     /locus_tag="VIL39_10505"
     CDS             complement(2959..3705)
                     /locus_tag="VIL39_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5481902.1"
                     /translation="MNPWRNIWTITKRELGAYFSSPIAYVFIVIFLLLCGFFTFFVGG
                     FFERQEASLVRPFFDWHPWFYLFLVPAVGMRLWAEERRTGTIELLLTMPITAWQAIVG
                     KFLASWLFLALALMLTFPVVITVNYLGHPDNGVILTAYLGSWLMAGAYLAISCVTSAL
                     TRSQVVSFIISLVVCLFLILAGFPPVINVLEQLAAPRLVDLVTSFSVMTHFEGFQKGV
                     LDSRDILFFLSVIGFSLFTTSVILRTHRAG"
     gene            complement(3702..4658)
                     /locus_tag="VIL39_10510"
     CDS             complement(3702..4658)
                     /locus_tag="VIL39_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5481903.1"
                     /translation="MIKVQNLVKVFGTKRAVDDLSFSVDRGEVLGFLGPNGAGKSTTM
                     RMITGFLPPSGGSVTVGGFDIVEQPIQARRLIGYLPENAPAYTDMTVKGFLNFTAEIR
                     GLRGDARTKAVSRAVEMCCLEAVLHQSVETLSKGFRHRTCFAQSIIHDPDVLVLDEPT
                     DGLDPNQKHEVRQLIRRMGEKKAIIFSTHILEEVDAVCSRAIIIDRGRIVANGTPQEL
                     RQKSEWAGAVTLRVKGVASSAVTAKLYQLPLVKRVTVVDDKEGHVTVRAFPKTGANGA
                     LAQAIGEAAHEWQIEQLQAEEGRLDEVFRSMTMPDTVKEATK"
     gene            complement(4914..5297)
                     /locus_tag="VIL39_10515"
     CDS             complement(4914..5297)
                     /locus_tag="VIL39_10515"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY5481904.1"
                     /translation="MAYRFQKHYTREEAGALLPQIRLWLKRMGQLRADVEKFETRLGG
                     LMVPGCDVGGQSVNTWVRSLADLEEVLLEFQRREIQLQDLDRGLLDFPAIIGGKEVFL
                     CWEQDEEDVEFWHELDAGYAGREHL"
     gene            complement(5334..7031)
                     /locus_tag="VIL39_10520"
     CDS             complement(5334..7031)
                     /locus_tag="VIL39_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5481905.1"
                     /translation="MLCRYWMWGVVLVTSSVLVAGCGSVPPRKAAPQKVSAAAALKRE
                     RAEDELAERRAQAHAHYSAGVIREMNNEQPAATEEYCQAALLDPGDEALTLEVTSRLL
                     QAKQPDRALAVLLRATAKPDASGEVYARLGLVYSQLGKADQAVAANRTAIRKSPGSLA
                     GYHNLFLGYLAGKQPQEALNALDEAARQPKPDADFLVGLADLYLSFGAQVPAQKDGAK
                     AKALAAVNRAAQLSPTTPLQRLKLADGFSLLGEPEKAAQLYLEVLKHPPDLPQVEERV
                     RAILTTIYLRSNDHKRAAEQLQAILRDDPTNPQARYYLGRLALEDKKPAEAADHFSKT
                     ILLSPGFESGYCFLALAQIEMDKISEALATLGNARQRFPQSFDLEFYTGLAFSRQKAY
                     AEAVQHYFAAEVIAQATDPQQLREGFYFQLGSACERKGDYVLAEKYFEKCLVLAPDFA
                     EAMNYLGYMWAEHGEKLDKARELIEKALKAEPKNDAYLDSMAWVLFKQKQPQEALPYA
                     LKAAELSEKPDATLHDHIGDIYEALKQPEKAREAWRKSLSLEPNEEVRKKLESSGPK"
     gene            complement(7112..7213)
                     /locus_tag="VIL39_10525"
     CDS             complement(7112..7213)
                     /locus_tag="VIL39_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEY5481906.1"
                     /translation="MGSIKKRRKAKINKHKRRKKLRANRHKKRTWQK"
BASE COUNT         1412 a         2370 c         2183 g         1607 t
ORIGIN      
        1 tcgagccggg ctttgtcctc ggccttttcg tccttgcccg gcgttcgctc tttggcgatc
       61 tgcgcggtta cggcgacaac catcggcaaa ttgtcgttgg tcttttggcc caccttaatc
      121 aggtaggtaa agttgtcgaa ggtatcaacc gtgataacgg tgggtttgtc caggccggtt
      181 tgctccggct ttgtgtcggc cggcaggaca tcggcaaagc tcggcgcatt aagggcatag
      241 gaaaagctgg aggtcttgga cgagtcgagt tgttccccgg gtttggcctc ggccagcttc
      301 cagtcgctgg cagtttcggt ttcccgtgtg accttccatg agttcgtggc caccgggtac
      361 gcgacggcaa tcgagcggat tttctctacc ttgaagaagt ccttgctcag ccacagatcg
      421 ggcttggctt cgaggttgct caggggatct gacaccacgg ccacagtctt ggctcccgtg
      481 ccgaccatga cgtaccgacc atcaggaaag ccttcgttgt cacccatctc gcccatcggc
      541 gagggacggc cctctgactt gcgcatgtgc gttttgccca gcagcaatga cttgatcacc
      601 ttgccgccct ggtcgcgcag ttccaccagc acggcggtat tggtccccgt ccccggcggc
      661 aggagcttat agcggcctaa ctgtgaggcg ccgacttctt ccgtttgggt cgctttgagg
      721 tccgccgctt tgagcagaaa accactgatc tcggagaagc tggccgggta atcgttgcgc
      781 tccttcaccc gccacaggtt gtccttcttg atgaggtcca actggttcgt gccgcctttg
      841 atgacgatgg acgcgacgtc attgaccggc agatcgccca gaagcttctg cgtggcgccc
      901 tgacgaccac ctccctgcca tgagctctgg ttacgctggt agagaaccag gcccgcgaca
      961 cccaacacca caactaacac cagtagaatg atgaattgtt tccgattcat tttgccgaag
     1021 tcttcctgcg gttaacaatc gcgatgacaa tgcccgttac cgtgaccgcc agcggcacgg
     1081 ccagaatatt gatccacttg aggcgcgttt ggagcgagac aacctctttc cgcaagtcct
     1141 tctgcacctg cttcagatgc ttgcgggctt ctgcctcttc cttgcgaagc ttctccaact
     1201 cggtgcgctg ctccggtgac aggatgaagc gctggtcttt gtccttcttc tgcgcctgca
     1261 tctcgttaat cttgcgctgc gattcagccg cgctttcctc caggcgcttg atttcactct
     1321 ggaacttctc gttggcagcc atttccatct ccttcacacg gataaaaggg cggatcaggg
     1381 aggcccggct ccggacggcg atgaggttgc tgtccccggt catctgttcg acgatgttct
     1441 gggcgaggtt gagattggca ttcatggcca tctgcatagc accaaacggg ctgtcgatcc
     1501 ggcgcatcgt gaacggatcg tagagcatgt cagcgtcacc aaacaacacc accgcgttgt
     1561 cgctcttggt ctccttgagt gagtcgccag gtttcttgtc ctccggcttc ttctcgtctt
     1621 tcttctcgcc gtccgacttg tcgtccttct tgtcctgcgg cgcgccgtcg gggaaggccg
     1681 tcttgaactt gccggtcagg cgaattgcga ggttgtagtt cacaccggag ggcttgaact
     1741 ccttcatgat gttgtcgccc gcgatattgg cgagcatggc gtccaccaac tgggagtcct
     1801 ttgagctgcg cagcagcacg gcttccttca gtcccgccac cggcgtgccg gtgaaggcgc
     1861 cggacaaagg cagccagatg ttgtcgagct gggcggtggc gacgtcatcc ttgtcaatgg
     1921 catccgcggt cagggccagc caggctggcg catcctgggg ctccccgccg cgcccgcgca
     1981 actgcaactt gtacttcaag tcagctacaa ccttgctggt gtcgaactga atcccccagg
     2041 ctttgggcag cttgtccagc gacgaaccac cgccgcccat gttggccatc atctggtttt
     2101 gctggcggtt gtcggccagg cactgggcat cgaggaacgc caccagcttg ccgccccgca
     2161 tgatgaactg gtcaatggca tattgcgcct tgtcggtaat atccttcggc gcgataacca
     2221 gcagcaactt gatgtcgtcg tcaatcttat ccacgtccat gccgacgcgc ttgacgttga
     2281 aatcgttctt cagctcgttg acgagggtcc agggctggct gccgcccggc ggttggccca
     2341 tttgctgcat catgggattg gagggcatgc cgaagaccgg cagcgggctc atgagcccga
     2401 tggtcggctt ctccggcgtc tcggcgcgga cgatagcgcg ggtcagatcg tattcgagca
     2461 ggcgctcgcg gttgggcgcc aggaacggaa ttgcctggac ttcatccgcg cacttgacgg
     2521 ccaagccgag atagatccgg tcggcgccgg ccagcgactg cggctcgatg ccgtccaacc
     2581 gtgccgagtc ctcggcgtcc gagtccggct gcgggtcgta tttctcgatc ttgaccttgc
     2641 tgcctgccac ctgtctgtat tcggtaagca ggtcctccac tttgcgcgca taacctttga
     2701 gaaacaccgt ctcgggcgtc gcactctcgc tctgtgtgca gtagaagcgg atggtgaccg
     2761 gcgtatccag cttcttcaga attgccttgg tcccggcgga aagggtgaaa gccttctcct
     2821 gcgtcaggtc caggcgcgtg cgcgcggcgc cggcaataaa gttgaagaga atcagaatgc
     2881 ccagcatcgc caggacgccg acagtcgaat atagaattgt ctgaaatgat ttcttttgca
     2941 tggatgcctc ccttgttttt agcctgcccg gtgggtgcgc aggatcacac tggtcgtaaa
     3001 gagcgagaac ccaataaccg acagaaagaa gaggatgtct cgcgagtcga gcacgccttt
     3061 ctggaaccct tcgaaatggg tcatcacgct gaaggacgtg accaagtcca ctagccgggg
     3121 agccgcgagc tgttcaagca cgttgatgac cggcgggaac ccagcgagga tgagaaacag
     3181 gcaaaccacc aacgagatga tgaaactgac gacctggctg cgcgtgagcg cggacgtaac
     3241 gcaactgatc gccaggtagg cgcccgccat cagccaactc ccgaggtagg ccgtcagaat
     3301 taccccgtta tcagggtggc caaggtagtt aacggtaatc accaccggaa aagtgagcat
     3361 gagggccagc gccaggaaca gccacgaggc caggaacttc cctacaatgg cctgccaggc
     3421 ggtgatgggc atggtcaaga gcaactcgat ggtgcctgtc cgccgctcct cagcccacag
     3481 acgcatcccc accgccggca cgaggaagag atagaaccac ggatgccagt cgaagaaagg
     3541 ccgaaccagg gaggcttcct gccgctcgaa aaacccgccc acgaagaacg taaagaaccc
     3601 gcatagcagc aggaagatca cgatgaagac gtaagcgatg ggcgagctga aataagcgcc
     3661 caactcgcgt ttggtaatgg tccagatgtt tctccaggga ttcacttcgt tgcctccttc
     3721 acggtgtccg gcatggtcat actgcggaaa acctcatcca ggcggccttc ctccgcctgc
     3781 agttgttcaa tctgccactc gtgggccgcc tcgccgatgg cctgggccag cgcgccgttc
     3841 gcgccggtct tcgggaaggc ccgcacggtc acgtgcccct ctttgtcatc caccacggtc
     3901 acccgcttga ccagcggcaa ctgatacagc ttcgcggtca ccgcgctcga agccacgcct
     3961 ttaacccgca gcgtcaccgc cccggcccat tcggacttct gccgtagctc ctgaggtgtg
     4021 ccgttggcca cgatcctgcc gcggtcaatg atgattgccc gcgagcagac ggcatcaact
     4081 tcctccagaa tgtgcgtcga gaagatgatc gctttcttct cacccatccg ccggattaac
     4141 tgccgcacct cgtgtttctg gttcggatcc agcccgtccg tcggctcatc cagcaccagc
     4201 acgtcagggt catgaatgat ggattgggcg aaacacgtgc gatggcgaaa ccccttggag
     4261 agcgtctcaa cgctttggtg caggaccgct tccagacagc acatctcaac ggcgcggctg
     4321 acggccttcg tcctcgcgtc accgcgcaag ccgcgaattt ccgctgtgaa gttgagaaac
     4381 cccttaaccg tcatgtccgt gtaagccggg gcgttttccg gcaggtagcc gatgagccgg
     4441 cgggcctgaa tcggctgctc cacgatgtcg aagcccccaa cggtcaccga cccgccggac
     4501 ggcggcaaaa agccggtgat catgcgcatg gtggtggatt tgccggcgcc gttgggcccc
     4561 aggaaaccaa gcacctcgcc tcggtccacc gagaacgaga ggtcatccac cgcccgtttg
     4621 gtcccgaaaa ctttcaccag gttctgaacc ttaatcataa tacatgttcc gcttctcacc
     4681 gaaatgaccg gtcgaaatat ccggttcagg ctcaaatgta aactagtttc ttcagcgagc
     4741 cggttggcca acgcggttac agtccgccgg gcgcttccgg catggttgga ttattctggt
     4801 caattagcgt tcaaaaaaag ccggagggtg tcccagaaac tgagctgggg aggggttggg
     4861 gccgcaacac gatgccaaca ctgagttcct gtcaccccgc tgcttcccct gccctacagg
     4921 tgctcccgcc cggcgtagcc ggcatccagt tcgtgccaga attcgacatc ctcctcgtcc
     4981 tgctcccagc ataggaacac ctctttcccg ccgatgatgg ccggaaaatc gagcaacccg
     5041 cggtccaggt cctgcaactg gatttcgcgg cgctggaatt ccaacaacac ctcctccaga
     5101 tcggcgaggc ttctgaccca cgtgttgacg gattggccgc ccacgtcgca gcctgggacc
     5161 atcaatccgc ccagccgcgt ctcgaacttc tccacgtcag cgcgcagttg gcccatgcgc
     5221 ttcaaccaca gccggatttg gggcaacaag gccccggctt cctcccgggt gtagtgcttc
     5281 tgaaatcggt aagccatggc gctcagctct gattgatcaa aggtgtctcc tgctcacttg
     5341 gggccgctcg actcgagctt ctttcggacc tcctcgttcg gctcgaggga aagcgacttg
     5401 cgccacgcct cgcgcgcttt ttccggttgc ttcagcgcct cgtaaatatc cccgatgtgg
     5461 tcgtgaaggg ttgcatcggg cttctcggac aactcagcgg ccttgagcgc gtagggcagc
     5521 gcctcctgag gctgtttctg cttgaagagc acccaagcca tgctgtcgag gtaagcatca
     5581 tttttcggct ccgccttgag cgccttctca atcagctcac gcgccttgtc caacttctcg
     5641 ccatgctcgg cccacatgta gcccagatag ttcatcgcct cggcgaagtc cggcgcgagc
     5701 accaggcact tctcgaagta cttctcggcc aggacgtaat cgcctttgcg ctcgcaggcg
     5761 gaccccagtt ggaaatagaa gccttcacgg agctgttgcg ggtcggtcgc ctgggcaatc
     5821 acttcggcgg cgaagtaatg ctggacggcc tccgcgtagg ccttttgccg gctgaaagcg
     5881 agcccggtat agaattccag atcgaagctc tgcgggaacc tctgcctggc gttgccgagc
     5941 gtggcgaggg cctcgctgat cttgtccatc tcgatctgcg ccagcgcgag gaagcaatat
     6001 ccggactcga agcccgggct cagcaggatt gtcttgctga aatggtcggc ggcttcggcc
     6061 ggcttcttgt cctccaacgc gagccggccc aggtaatagc gggcctgggg attggtcggg
     6121 tcgtcgcgca ggatggcctg gagttgttcg gcagcccgct tgtgatcatt gctgcggaga
     6181 taaatggtgg tcaggatagc ccgaactcgc tcctcaacct gcggcaagtc aggtgggtgt
     6241 ttgagcacct cgaggtaaag ctgcgcggct ttctctggct cgccgagcag gctgaagccg
     6301 tccgccagct tcaggcgttg cagcggagtg gtggggctca gttgcgccgc gcgattcacg
     6361 gcagcgagag ctttcgcctt ggccccgtcc ttttgcgccg gcacctgcgc cccgaaactc
     6421 aggtacagat ccgccaggcc gacgaggaag tcggcgtcag gtttcggctg ccgggcggcc
     6481 tcatcgagcg cgttgagcgc ctcctgcggc tgtttgccgg ccaaatagcc gaggaaaaga
     6541 ttgtggtaac cggcgagcga gcccggcgat ttccggatgg ccgtgcggtt ggccgcaacg
     6601 gcctgatcag ccttgccgag ctgggaatag accagcccaa gccgcgcgta aacttccccc
     6661 gaagcgtccg gcttagccgt ggcacgcaat agcacagcca gcgcccggtc gggttgcttc
     6721 gcttgaagca agcgactggt gacctcaagg gtgagcgcct catcacccgg gtccagcaat
     6781 gccgcctggc aatactcctc cgtggcggct ggctgctcat tgttcatctc ccgaatgaca
     6841 cccgccgagt agtgcgcatg agcttgggcg cgcctttccg ccaactcgtc ctccgcccgc
     6901 tcgcgcttaa gggcagccgc ggcactaacc ttctgaggcg ccgctttgcg cggcggcaca
     6961 gaaccacacc cggccaccag cacgctgctt gtgaccaaga ccactcccca catccaataa
     7021 cgacacagca tgtggataaa aggcgacccg tcccgctccg ggaacaggtc gctttgaatc
     7081 aactcaccgg cagccccgca gggccttccg gttacttctg ccaggtgcgt ttcttatgcc
     7141 gattagcacg cagcttcttg cgccgtttat gcttattgat ctttgctttc cgacgtttct
     7201 ttattgaacc cataaacaat ttccgtattc ctgctcaccg gtctcgctca gccggcgcga
     7261 cgcacctgcg cctgcccgca actctctatg agaaccaacc gatcgtgttc agttgccata
     7321 tcgtgcggga ataaagtgct atcgtcaagc gcgttcacaa tcgcgcgctc gcggagggcg
     7381 caggacagaa ttcttccatg cagacccgag gctgctctgc ggacgcggtt gagaggccga
     7441 aggcgtcatt cataccagcc cagggcaaac gccctgggtt cattgcccta aagacggtct
     7501 ccagcgctga aggcgcgact catccttcct cggcttcaag agccacagcc tcaaaccgat
     7561 gaagcaggcc tt
//
LOCUS       DATCGS010000513         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_736655, whole genome shotgun sequence.
ACCESSION   DATCGS010000513 DATCGS010000000
VERSION     DATCGS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736655"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1995
                     /locus_tag="VIL39_10530"
     CDS             169..1995
                     /locus_tag="VIL39_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HEY5481907.1"
                     /translation="MDADIVCVGFGPAMAGFLTGLSKHLVNPDGTPAVESAVMPGMPP
                     QVLCYERADDIGFGVSGVVTRGRGLRATFPDLDPKQIPMATAVTEEKVVYLLDPIGAS
                     RRSWTLRAADSCVRAFRRLLPFEHEAIELPWSPPFLHKTGGLVLSMGQFMQWVGAQVQ
                     STGTVQIWPGTPVTQALVENQKVIGIRLLDQGVDKQGKPGDGFMPGMDIHAALTVVGD
                     GPVGPIGQQLDAQFGLPEGHHVRDWAVGMKFVVDLPADTTLKPGTVLHTFGFPEPEIF
                     GFLYVHPDRVASLGIFVPSWFDTPMRTAYRYLQHWMLHPYLWRYLKGSKLRSWGAKTL
                     GESGRRGEPHLVGDGYARVGEGSGSTNVLTGSGVDEAWVTGSQLAESVLELLKSKKPF
                     TRQHLEETYLKRRRASWVEAEGRVAEKSRDGFQKGVVRGMVGMALAGLTNGRLTLSGE
                     PVQPYKRIPTLEEYYRGRIPEAEIQQLRKECAIKGVSLHSALMQRCGWPAIPYDGQLL
                     VSHQDALLLGGKVQAPPGYADHVVFLYPELCERCGMKICIELCSGQAIAPGTGGVPTF
                     DREKCVHCGACYWNCTQPIPENPERMNIAFRAGTGGLHSAEN"
BASE COUNT          420 a          708 c          682 g          407 t
ORIGIN      
        1 acacgctgta taaggccaaa ccactcggtc gatctcaaga tgggcgttca gcggcacacc
       61 atgacgtaat actgtatccc cgatgaagtt gtgaacgtgg aaaaccagat caccacctca
      121 cgaaatacca gcgtcacagc cgctccgccg tcaattgacc gccagcgcat ggacgcggac
      181 atcgtctgcg tcgggttcgg accggccatg gcgggattcc tcaccgggct ctcgaagcac
      241 ctcgtcaacc cggacggcac gcccgcggtg gaaagtgcgg tcatgcctgg catgccgcca
      301 caggtcctgt gctatgaacg cgcggatgac atcggcttcg gagtctccgg cgtcgtgacg
      361 cgagggcgcg ggctgcgggc cacattccct gatctcgacc ccaagcagat tcccatggcg
      421 actgccgtca cggaagagaa agtcgtctat ctgctcgacc cgataggcgc gagccgacgc
      481 tcatggaccc tgcgcgcggc ggattcctgc gtccgggcgt ttcggagact gctgcctttc
      541 gagcatgagg cgatcgaatt gccgtggtcg ccgcctttcc tccacaagac gggtgggctg
      601 gtgctctcga tgggccaatt catgcagtgg gtcggtgccc aggtccagag caccggcacg
      661 gttcaaatct ggcccggcac acccgtgaca caggcactcg tcgagaatca gaaggtcatc
      721 ggcatccgct tgctcgacca aggggtggac aaacaaggca agccgggtga cggcttcatg
      781 ccaggcatgg acatccacgc ggcgctcacc gtggtcggcg acggcccggt tggccccatc
      841 ggccagcagc tcgacgcgca gttcggcctg cccgaaggcc accatgtgcg cgattgggcc
      901 gtaggcatga agtttgtcgt ggatctgcct gccgatacaa cgctcaaacc gggcaccgtc
      961 ctccacacgt tcggcttccc cgagcccgag atcttcggct tcctctacgt gcaccccgat
     1021 cgtgttgcct cgctcggcat cttcgtgcca tcatggttcg acaccccgat gcgcacggcc
     1081 taccggtacc tccagcactg gatgctgcat ccgtatctct ggcgctacct caaagggagc
     1141 aagctccgtt cctggggcgc caagacactc ggcgagtcgg gccgccgcgg cgagccgcac
     1201 ctggtcggcg atggttacgc gcgcgttggc gaaggctccg gcagcaccaa tgtcctcacc
     1261 ggctccggcg tggacgaagc ctgggtgacc ggctctcaac tggccgaaag cgtcctcgaa
     1321 ctcctcaagt cgaagaagcc ctttacccgg caacacctgg aagaaaccta tctcaaacgc
     1381 cgccgcgcga gttgggtgga agcggaaggg cgcgtcgccg agaaatcacg cgacggcttc
     1441 caaaagggcg tggtgcgcgg catggtgggt atggcgctgg ccggcctgac caatggccgg
     1501 cttacgttga gcggcgagcc cgtccagccc tacaaacgca ttcccacgct ggaagaatac
     1561 taccgcggcc ggattcccga agccgaaatc cagcagctcc gcaaggagtg cgccattaaa
     1621 ggcgtgtccc tgcacagcgc cctcatgcag cgttgcggct ggccggcgat cccctatgac
     1681 ggccaattgc tcgtatcgca ccaggacgcg ctgttgctcg gcggcaaggt ccaggcgccg
     1741 cccggctacg ccgatcatgt cgtctttctc taccccgagc tttgcgagcg ctgcggcatg
     1801 aagatctgta tcgagctctg ttccggccag gctatcgccc ccgggacagg tggcgtgcca
     1861 accttcgatc gcgagaaatg cgtgcactgt ggggcctgtt actggaactg cacacagccg
     1921 atcccggaaa accctgagcg tatgaacatc gcctttcgcg ccggcaccgg cgggctgcat
     1981 tcggcggaga attgaccgcc aaggcggcgc ggttgacaac atatcatata ggatatatgc
     2041 tgaacttgtt gcttggttgt gtccggttgg aaagcggacg gtgccctttc cttgacggct
     2101 tggaagaact gccggtcatg gatcaggcga aggttctggc ttgggccaag cgactggctg
     2161 tccatgaaga gttgcggcga ccgccttatg cgaaacattt ggacgatggc atatttg
//
LOCUS       DATCGS010000514         4965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_489802, whole genome shotgun sequence.
ACCESSION   DATCGS010000514 DATCGS010000000
VERSION     DATCGS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489802"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..522
                     /locus_tag="VIL39_10535"
     CDS             280..522
                     /locus_tag="VIL39_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY5481908.1"
                     /translation="MTPAERQRRIERFETAHRESGLPVTMQRRAVFEAILDRRDHPTA
                     DQVFHALHPRLPQLSRMTVYRILGTLVSLDLRLTLS"
     gene            678..1256
                     /locus_tag="VIL39_10540"
     CDS             678..1256
                     /locus_tag="VIL39_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY5481909.1"
                     /translation="MSKSLKGTVTEQNLLKSFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFLETASQEIEHAKRFFKFLEGGMTEIQASYPAGVIGTTKENLKAAAEGENEEWTTL
                     YPKFAETAQKEGFPEVAAAFRMITKVEAEHERRYLKLLQNVSEDRVFVKDGKVWWQCR
                     KCGYVYESDKALEVCPACLHPKAYMQVRSENY"
     gene            complement(1274..1588)
                     /locus_tag="VIL39_10545"
     CDS             complement(1274..1588)
                     /locus_tag="VIL39_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481910.1"
                     /translation="MNPSLLNEILDLMNDGRVHSKESLVSALGTSAAMADMMIEHLIR
                     MGYLEQIGGESAPDLCSLCDQNRGSRTHCTLCHQQGRALLLTRKGIKQAAAPEARPGT
                     QG"
     gene            complement(1585..3867)
                     /gene="feoB"
                     /locus_tag="VIL39_10550"
     CDS             complement(1585..3867)
                     /gene="feoB"
                     /locus_tag="VIL39_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEY5481911.1"
                     /translation="MMQAANGRGGNTLSENGGKGARSLAEVPVDFTGIVCDLSGDAKR
                     RQRLVSLGFTPGALIQVLRTGDSGPMLVSIRGSRVALGLDEAGLVEVGEAPPEPNPPE
                     VTNQKPVVIALAGQPNVGKSTLFNTLTGLRQHVGNWTGKTVELKSGELVFQQTAFTLV
                     DLPGTYSLTAASEEERIARNFILHEKPDVVVAIVNATNLERGLYLVAELLSLPSPVIL
                     ALNMMDIAEEAGFSIEPKVLETAIGIPVVPMSAAKGQGVAELMETIVQLREDKLPYHP
                     QKPTILAAHQKVLDEVMGLLDGIGLEGLPLRWVGIKLLEGDEEITRKVKALVRPEVWQ
                     KVERLLYTHEDAILDIAGARYTWIARMLRAAVFEPPISHGRLTSRLDRVLTHPFWGTL
                     VMLLLLGGVFWLTFALGSPMQNWLARLVSNLADKMRDWLSFAPKWTVELIAGGALGGL
                     GMVLTFLPLLALFYTVLGILEDTGYLSRIAFLADRWMHRLGLHGKSFLPLLLGFGCNV
                     PAILGSRIVESPKARLLTLLLIPLIPCAARTAVVTFLAPVLFGPSAAIVALGLLAGNL
                     MLLGLAGFVLHRFVFHNEHVPFIMELPLYHVPNAKTIALFVWNNLVSFLQKAGKVILF
                     ASIIVWALSYFPNGNINTSYLARVGMLLEPFGKLMGLPWPALVALLTGAVAKENTIAT
                     LGVLYGNIGQTLPPVLGTAGSLSMLVFQMLFIPCIATLAAMRQETRSWKWTLASAGMM
                     IVLSTVASIVVYQLARVLLG"
     gene            complement(4149..4415)
                     /locus_tag="VIL39_10555"
     CDS             complement(4149..4415)
                     /locus_tag="VIL39_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481912.1"
                     /translation="MSDHAHSVRFHARKPWLLITAAPGVMVVDTRMVLANFNAPHPER
                     ITALSFNQDGRLLAALTDNREIQLWDLGLFLALPAREADTDAPV"
     gene            4641..>4965
                     /locus_tag="VIL39_10560"
     CDS             4641..>4965
                     /locus_tag="VIL39_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481913.1"
                     /translation="MRQFVLCRLICALSVALAVLLSGCVRDLEQAPGSLVPPQSVGRV
                     SANLYQTPNGQMLTPTGRQIALPGMRPQALALSPKGNLLATSGKSQTLVLIDPANGHI
                     LQTVQL"
BASE COUNT         1060 a         1538 c         1448 g          919 t
ORIGIN      
        1 aagccgcagc gcggacacgg cccggcgcga agcagctcaa gcagccggcc ccacgcctgc
       61 aagcaatgga tgctcaccgg cacgttgcgc gacgccgctt tccctgaccg cagtaatccg
      121 ctgatgcagc agaagtgcca cccgcagcca aagcggcgtc gcgcttcgct tgccgccgca
      181 ctccatagcg actttgtcaa tggccgtgta aacacttcca acccacccaa aaacctattt
      241 gcatatttgt atcatccggt atagttaagc attcgtataa tgactccggc agagagacaa
      301 cgacgcatcg agcgctttga gaccgcccat cgcgagagcg ggttgccggt gaccatgcag
      361 cgacgtgccg tgttcgaggc gatcctggac cgccgggacc acccgaccgc cgaccaggtt
      421 ttccacgccc tccatccgcg cctgccccaa ctctcgcgca tgaccgtcta tcgcattctg
      481 ggaacactgg tctcccttga cctccgtcta accttaagct gaaaggagcg agaacatgaa
      541 gaagacgcaa ttgaccacca cgagcggaaa caccaaccac caatttcaaa tgcctgactc
      601 gcctgggtgt gcctgatctg cgggagcggg caaagatgat ttcgttaaag ttccgtaacc
      661 attaaaacca agttaatatg tcaaaatcac taaagggcac tgtaacagaa cagaatctgc
      721 tgaaatcatt cgcgggcgaa tcgcaagccc gcaaccgcta cgaattcttc gccagcgtgg
      781 ccaagaagga aggatacgag caaatcgcgg ctattttcct cgagaccgcc agccaggaaa
      841 tagagcatgc caagcgtttc ttcaagttcc tcgaaggcgg tatgacggag attcaagcgt
      901 cgtatcccgc cggcgtcatc ggcacgacca aggagaacct caaagccgcc gccgagggag
      961 agaacgagga atggaccacg ctctacccga agttcgctga gactgcccag aaggaaggtt
     1021 tcccggaagt tgccgctgcc ttcaggatga tcaccaaggt tgaggcggag cacgaacgcc
     1081 gttacctgaa gctgctccag aatgtctcgg aggacagggt gtttgtgaag gacggcaagg
     1141 tatggtggca gtgccgcaaa tgcggctacg tgtacgagtc ggacaaggcc ctggaagtct
     1201 gccccgcttg cctgcatccc aaggcgtaca tgcaagtgcg cagcgagaat tactagccca
     1261 ataggaaacc gaatcacccc tgggtgcccg gcctggcctc tggggcagcc gcctgcttta
     1321 ttcctttgcg cgtcagcagc aaagcccggc cttgctggtg gcagagggtg cagtgtgtcc
     1381 gggagccgcg gttttgatca cacagagaac agaggtcggg ggctgactcg ccgccgattt
     1441 gctcaagata ccccatgcgg atgagatgct cgatcatcat gtccgccatt gccgcgctgg
     1501 ttccgagggc cgaaaccagg gattctttgg aatgcacccg gccatcgttc atcaggtcga
     1561 gaatctcgtt cagcagggaa ggattcatcc gagcaagacc ctggcgagct gatagaccac
     1621 gatggaggcc acggtcgaga gaacgatcat cataccggcg ctggcaagcg tccatttcca
     1681 ggagcgggtt tcctggcgca tcgcggccaa cgtggcgatg catgggatga agagcatctg
     1741 gaagacgagc atggagaggg agccagccgt ccccaggacg ggcggcagcg tctgaccgat
     1801 gtttccgtag agaacgccca aggtcgcgat ggtgttttct ttggctacgg ccccggtgag
     1861 cagcgcgacc agcgcaggcc atggcaggcc catcaatttg ccgaagggct ccagaagcat
     1921 tcccacgcgc gccagatagc tggtgttgat gttgccattc gggaaatagg acaacgccca
     1981 aacgattatt gatgcgaata agatcacctt cccggctttt tggaggaagc tcacgaggtt
     2041 gttccaaacg aagagggcaa tcgttttggc attgggaaca tgatacaggg gcagttccat
     2101 gatgaagggg acatgctcgt tgtggaagac gaaacggtgc aggacaaatc ccgccagccc
     2161 cagcagcatg agattgcccg ccagcaaccc caacgcgact atcgccgccg aggggccgaa
     2221 gagcaccggc gccaggaacg tcaccacggc ggtccgcgcc gcgcagggaa tcagcgggat
     2281 gagcagcagc gtcagcaggc gggctttggg cgattccacg atgcggctgc ctagaattgc
     2341 cggcacgttg cagccaaacc ccagcagcaa gggcagaaag ctctttccat gcagccccag
     2401 gcgatgcatc cagcggtcag ccaggaaagc gattcgggac aggtagcccg tatcttccag
     2461 aatccccagt accgtgtaaa agagcgccag caggggcaga aacgtgagca ccattccaag
     2521 gccgcccagg gcgcctccgg cgatgagttc gaccgtccat ttgggagcga agctgagcca
     2581 gtcccgcatc ttgtcggcca ggttgcttac cagccgcgcc agccaattct gcatggggct
     2641 gcccaaggca aaggtcagcc agaacacccc gcccagcaac agcaacatta ctagcgtgcc
     2701 ccagaacgga tgggtcagca cccggtccag gcgggaagtg aggcgcccgt gagaaatcgg
     2761 aggttcgaac accgccgcgc ggagcatccg ggcgatccag gtgtagcgcg ccccggcgat
     2821 gtccagaatg gcatcttcgt gcgtgtaaag aagccgttcg actttttgcc agacctcggg
     2881 tcgaaccagg gccttgacct tgcgggtgat ttcctcgtcg ccttccagca gcttgatgcc
     2941 tacccatcga agcggcagcc cttccaggcc aatcccgtcg agtaacccca tcacctcgtc
     3001 gagcaccttt tggtgcgccg ccaggatggt gggtttttgt gggtggtacg gaagcttgtc
     3061 ctcccgaagc tggacgatgg tttccatcaa ctccgccaca ccctgtcctt tggccgcgga
     3121 catcggcacg acggggatgc cgatggccgt ttcgagcact ttcggttcga tcgagaatcc
     3181 cgcttcctcg gcaatgtcca tcatgttcag cgcgaggatg accggcgaag gcaaggagag
     3241 caattcggcc accaggtaca gccccctctc cagattggtg gcgttgacta tggcaacgac
     3301 cacgtcgggc ttttcgtgca ggatgaaatt gcgggcgatg cgctcctcct cggacgccgc
     3361 cgtaagacta taggtgccgg gcaaatctac cagcgtaaag gccgtctgct gaaaaaccag
     3421 ttccccggac ttgagttcga ccgtcttccc ggtccaattg cccacatgct ggcgcagtcc
     3481 cgtcagtgtg ttaaacagcg tggattttcc aacgttcggc tgccccgcca acgcaatgac
     3541 caccggtttc tggttggtca cttccggtgg gtttggttcg gggggcgcct ccccgacttc
     3601 cacaagaccg gcttcgtcca gacccagcgc aacgcgcgag cctcggatcg agaccagcat
     3661 cggcccgctg tcaccggtgc gcagaacctg gatcagcgcg ccgggggtga aaccgagaga
     3721 gaccagccgc tgtcggcgtt tggcgtcacc ggacaaatcg cacacgatgc cggtgaaatc
     3781 cacgggcacc tccgctaaac tcctggcgcc tttgccacca ttctcgctca gcgtgtttcc
     3841 tccccgcccg ttggccgcct gcatcatagc agtcctctcc gcctgaagcg gtagcggcgc
     3901 gcgcgcgcga aatattgggc ggggtaagcc atacgtttcg tcaccctatt ctacagggtt
     3961 agcagcgctt gtccatatcg ccgcatctgc tccggagccc aagagcaacc ggcagggcac
     4021 ccggaagcct cgcttctcca ctcctcttcc tggtatgccc gcttgaaata agtggtgtag
     4081 atgcctccgc cgagcagtcc gccgaccacc ggcgtaaacc acctcgccca cctcacgccg
     4141 taaccccttc aaaccggtgc gtcggtgtcg gcttcccggg ccggaagtgc tagaaacaac
     4201 cccaggtccc agagctgaat ctcccggttg tccgtgagtg ccgccagcag acggccatcc
     4261 tggttgaagc tcaacgcggt gatgcgctcc ggatgcggcg cattgaaatt ggccagaacc
     4321 atccgcgtgt ccaccaccat cacccccggt gccgcggtta tgagcagcca gggtttgcgc
     4381 gcatgaaacc gcacgctgtg cgcgtggtcg ctcaacggtc gctctcgccg ataaacaccc
     4441 gcagcaacat cgcttcatgg ggtagatgca tatcattctc ctttgccgaa aaaactggaa
     4501 gcgccccacc gggcgggacg cccggtcaac tcgcaggcga gatgacatgc gctacacttt
     4561 gtcaccggcc ctgcatctgg cttaggtgcg tggccaacga ggttgacttt tcaccgggcc
     4621 tttaggttaa gatatggagg atgcgacaat ttgtcctctg ccggttgatc tgcgccttga
     4681 gtgtggcgct cgccgtcctt ttgtctggat gcgtgcgcga cttggaacag gcgcccggat
     4741 cactggtacc gccgcaatct gtcggacgcg tgtcggcaaa cctgtatcaa actcccaacg
     4801 gccaaatgct gacccccact ggccgccaga ttgcgttgcc gggaatgcgt ccgcaggcgc
     4861 tcgcgttgag tccgaagggc aacctgctgg cgaccagcgg caagagccag acgctggtgc
     4921 tcattgatcc ggcgaacggt catatcctgc agacggtcca gctct
//
LOCUS       DATCGS010000515         3581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_588617, whole genome shotgun sequence.
ACCESSION   DATCGS010000515 DATCGS010000000
VERSION     DATCGS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588617"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="galK"
                     /locus_tag="VIL39_10565"
     CDS             complement(<1..1135)
                     /gene="galK"
                     /locus_tag="VIL39_10565"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY5481914.1"
                     /translation="MTLNELSRHTAQLFTKTYGCPPRWIAAAPGRVNVIGEHTDYNDG
                     FVLPMAIDRYTVIAAAPAKHGATKVQLRSTVGDGAISLDLTQPVKPAPKGTWSNYPVG
                     VIAGFLARGAKPCGFDALIHSTVPLGGGLSSSAALEVSTATLLEAITGQKLDPVDKAL
                     LCQKAEHDYAGMPCGIMDQFISVMGKQDHLLLLDCRSRKTELVPMTDLSKAILIINTN
                     VKHELTGGEYAKRRAQCEQAAKALGVTSLRDATADMLVRARGRMEEVVFRRARHVIGE
                     IERTPHAAEAVRASNWPTVGQLMYASHASLRDDYEVSCPELDAVVDIAQAIGPKGGVI
                     GCRMTGGGFGGCAIALVHTDAVSAISDRVASEYERRTKIKPTLF"
     gene            complement(1143..2186)
                     /locus_tag="VIL39_10570"
     CDS             complement(1143..2186)
                     /locus_tag="VIL39_10570"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY5481915.1"
                     /translation="MQARVLKSSFGKLPDGTAVDLYTLTNANGLSAKVATYGTIITEL
                     HVPDRRGELGDVVLGFDNLEQYLKGHPFFGATVGRVANRIAKGRFTLDGKTCTLATNN
                     GPNHLHGGLKGFDKKVWQAEPQTGAAVKFSYTSPDGEEGYPGTLAVAVTMSLTDANEL
                     RLDYTASTNKPTPVNLTNHSYFNLAGEGSALDHEMMIAADYFTPSDSTLIPTGEICPV
                     KGTPMDFTTPQPIGSRFSQLQTKPLGYDHNYVLNAGGKGLGLAARVQERNSGRVMEVH
                     TTQPGVQFYSANFLNGSLTGKRGIVYPQHCAFCLETQHFPDSVNQPKFPSVILRPGQT
                     YRQTTVHKFSTQP"
     gene            complement(2192..2425)
                     /locus_tag="VIL39_10575"
     CDS             complement(2192..2425)
                     /locus_tag="VIL39_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481916.1"
                     /translation="MGLDIRWPIGLLFSLVGALLVIYGLATGSDTEIYRRSLDINVNL
                     RWGLVVLAFGAAMLTLAWRASRQPAEPQAPEKK"
     gene            complement(2444..>3581)
                     /locus_tag="VIL39_10580"
     CDS             complement(2444..>3581)
                     /locus_tag="VIL39_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HEY5481917.1"
                     /translation="LVVQRAMAARNMSAARRTPLIAAVPKMIFPILVILPGMIAAGLA
                     ITAKDGYRLPPKTLDSAIYAKAIPAVAEAMAKHLTGAAAAQLVSEAAGVKMLPDKIDA
                     LMKAAPTLKGKELEEGLQNAVAENNYDGVILSLVQRYCPSGLLGLALTGLLASFMSGM
                     AGNVTAFNTVWTYDLYQAYIAKNKSDAHYMWMGRCITVVGILLSIVCAYLVSNWSNAM
                     DIIQLVFGFVNAPLFATFLFGMFWKRTTSTGAFLGLLGGTGTSALFHSLSSASGNALG
                     IKGGYLWSLMGTKLADVPHCLIFPSDMAQNFWLASFAFTVCFVLTLVISLATRRTKTD
                     EELKGLVYSLTPKIKDEEEAWYLRPAVLGVVLLACCVILNIIFW"
BASE COUNT          637 a         1032 c         1231 g          681 t
ORIGIN      
        1 cgaagagggt gggcttgatt ttggtgcggc gttcgtattc ggaggcgacg cggtcagaga
       61 tggcgctgac tgcgtccgtg tgcactagcg ctatggcgca gccgccgaag ccgccgccag
      121 tcatccggca gccaatgaca ccgcctttgg ggccaatggc ctgcgcgatg tccacgacgg
      181 cgtccagttc cgggcagctc acctcgtaat cgtcgcggag cgaggcgtgg ctggcataca
      241 taagctggcc aacggtcggc caattcgagg cgcgcactgc ttccgccgca tggggagtcc
      301 gctcgatctc accgatgacg tgccgggcgc ggcggaagac cacctcttcc atcctgccgc
      361 gcgcgcgcac tagcatgtcc gcggtggcat cgcgcaggga ggtcacgccc agggcttttg
      421 ctgcctgttc gcattgagcg cggcgtttgg cgtattcgcc gccggtcagc tcatgcttga
      481 cgttggtatt gatgatgagg atggccttgg acaggtcggt catggggacc aattcggttt
      541 tccgggaacg gcagtcaagc aggagcaggt ggtcctgctt gcccatcacg gagatgaact
      601 ggtccatgat gccgcaaggc atgccggcgt agtcgtgctc cgccttctgg cagagcagcg
      661 ctttgtccac cgggtcgagc ttctggcccg tgatggcctc gagcagggtc gcagtggaga
      721 cttccagcgc cgcgctgctg gacaggccgc cccccagcgg gaccgtggaa tggatcaagg
      781 catcaaagcc acatggcttc gcaccgcggg ccaggaagcc cgcgatcacg ccgaccgggt
      841 aattggacca cgtgcccttc ggggcgggtt tcaccggctg ggtcagatca aggctgatcg
      901 ccccgtcgcc gaccgtagag cgcagttgga ctttcgtggc gccatgtttg gcgggcgcgg
      961 cggcgatgac cgtgtaacgg tcgatggcca taggcaggac gaacccgtca ttgtaatcgg
     1021 tatgctcgcc gatgacgttg acgcgcccgg gagcggcggc gatccagcgg ggcgggcatc
     1081 cgtaggtctt agtgaatagc tgcgcggtgt ggcggctcag ttcgttgagg gtcatacgag
     1141 agtcagggct gggttgagaa cttatggacg gtggtttggc ggtaggtctg accggggcgc
     1201 aagatcaccg aagggaactt cggctggttg accgaatccg ggaagtgctg cgtctccagg
     1261 cagaacgcgc agtgttgggg atacacgatc ccgcgcttgc cggtcagcga cccattgaga
     1321 aagttggccg agtagaactg aaccccgggc tgggtggtgt ggacttccat cacacggccg
     1381 ctgttgcgtt cctgaacgcg ggccgccagc cccagaccct tgccgccggc attcagcacg
     1441 tagttgtggt catagccgag gggcttggtc tgaagctgcg agaagcgcga gccaatgggc
     1501 tgcggggtcg tgaaatccat cggggtgccc ttgaccgggc agatctcccc ggtggggatc
     1561 agggtgctgt ccgacggcgt aaagtagtcc gccgcgatca tcatctcgtg gtcgagcgcg
     1621 ctgccttcgc cggcgaggtt gaaataggag tggttggtca ggttaaccgg cgtgggcttg
     1681 ttggttgagg cggtatagtc gaggcgcaac tcgttggcat cggtcaggct catggtgacg
     1741 gcgacggcta gtgtgccggg gtagccttcc tcgccgtccg ggctggtgta gctgaacttc
     1801 accgccgcgc cggtctgcgg ttcggcctgc cagacctttt tgtcgaagcc cttgaggccg
     1861 ccatggagat ggttcgggcc gttgttagtc gcgagggtgc aggtcttgcc gtcgagcgtg
     1921 aagcggcctt tggcaatgcg gttggccacc cgtccgacgg tggcgccgaa aaaaggatgc
     1981 cccttcaggt attgctcgag gttgtcgaag cctagcacga cgtcgcccag ctcgccccgg
     2041 cgatccggca cgtgcagttc agtgatgatc gtgccgtagg tggcaacttt ggcgcttagg
     2101 ccgttggcgt ttgtcagggt gtacaggtcc actgccgtgc cgtcgggcag tttgccaaag
     2161 ctggacttga ggacgcgcgc ttgcatccgc gtcacttctt ctccggcgcc tggggctcgg
     2221 cgggctggcg gctcgcgcgc caggccaatg tgagcatggc tgcgccgaag gcgagcacca
     2281 ccaggcccca gcgcaggttg acgttgatgt ccagcgagcg ccggtaaatc tctgtgtcgg
     2341 agccggtcgc caagccgtag atcacgagca gggcgccgac cagtgagaac aagaggccga
     2401 tcggccagcg aatatcaaga cccataatgt tgttcctttc gtgttaccag aaaatgatgt
     2461 tgaggatcac gcagcaggcg agcaggacta cgcccagcac cgccgggcgc aaataccacg
     2521 cctcctcctc gtccttgatc ttgggcgtta gcgaatagac caatcccttg agttcctcgt
     2581 cggtcttggt ccggcgggtg gccagggaga tgaccagcgt caggacgaag cagacggtga
     2641 aggcgaagct ggccagccag aagttctggg ccatgtcgct ggggaagatg agacagtgcg
     2701 ggacatcggc gagcttcgtt cccatcagac tccagagata gcccccttta atgccgaggg
     2761 cgttgcccga tgcagaagac agcgagtgga agagcgccga agtgccggtg ccgcccagca
     2821 aaccgaggaa cgcaccggtg ctggtcgtac gtttccagaa catgccgaac aggaacgtcg
     2881 cgaagagcgg ggcattgacg aagccgaaca ccaattggat gatgtccatc gcattggacc
     2941 agttgctgac aaggtaggcg cagacgatac tcaacaggat gcccacgacg gtgatgcacc
     3001 ggcccatcca catgtagtgc gcgtcgctct tattcttggc gatgtaggcc tggtaaaggt
     3061 cataagtcca gacggtgttg aaggcggtga cattgccggc catgcccgac atgaaggacg
     3121 ccagcaatcc cgtcagcgcc agccccagga ggccggaggg acagtacctc tgcacgaggg
     3181 acaggatgac gccgtcgtag ttgttctccg ccaccgcgtt ctggagccct tcctccagtt
     3241 ccttgccctt aagcgtcggg gcggctttca tcagggcgtc aatcttgtcc gggagcattt
     3301 tcaccccggc ggcctcggag accagttggg cggcggcggc gccagtcaga tgcttcgcca
     3361 tcgcttccgc gaccgccggg atggctttgg catagatcgc ggaatcgaga gtcttgggcg
     3421 gcaggcggta gccgtccttg gccgtaatag ccaggccagc ggcgatcatg ccgggcagga
     3481 tcaccaggat agggaaaatc atcttgggca cggcggcaat gagcggagtg cggcgcgcgg
     3541 cgctcatgtt tctggcggcc atcgcgcgct gcacgacgag g
//
LOCUS       DATCGS010000516         5221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_193719, whole genome shotgun sequence.
ACCESSION   DATCGS010000516 DATCGS010000000
VERSION     DATCGS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193719"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1427)
                     /locus_tag="VIL39_10585"
     CDS             complement(213..1427)
                     /locus_tag="VIL39_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481918.1"
                     /translation="MSVRSIAEKAIEQGKGILRLAPNWVPRSFCVPGRRIKLHPADYY
                     ALGGDRGGIDERWFSSTTPADNGPKTSPNEGLSFIVVEDGSQTEKVTLRDAVSELKGS
                     IIGARLWDAYHCWPMYSKFFDNKGPLPHHIHHREQHAKLVNQMGKPESYFFPVQLNNY
                     GADFPYTFFGLQPGTTKDQLRECLQNFNKGDNKITNLSAAYRLEPGTGWNVQAGILHA
                     PGSLCTYEPQKASDVFAMYQSLTGDAIIPEELLWKNTPPDKVGNIDHLIEVVDWEANT
                     DPNFAAHHFRRPKPVRSIEQMRAEGCQENWITYDAEDYSAKELTVLPGKTVTIKDGGA
                     YGIILLQGHGKMGVWDIDTPAIIRYGQLTMDEFFITEDAARAGVVISNPSKTDPIVML
                     KHFGPGNPDMKK"
     gene            1617..2729
                     /locus_tag="VIL39_10590"
     CDS             1617..2729
                     /locus_tag="VIL39_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEY5481919.1"
                     /translation="MKSALLASLVLVSVVVGASASTEPPYPHSQVLQSIAWHWETYTT
                     AALGSDLWPVAWGPDDQLYTAWGDGGGFGGGDKDGRVALGFARIEGTPEHWRGVNVNG
                     GKNPEHPASFPRKGKTSGLAIVDGIFYATVNLQDGTWPNVNHVLAWSTNYGATWSKAE
                     WLFARGEGSFQPATFLTFGRDYTGAPGPLAGYVYIYGPKLSADRGSGQWLYLARVPRN
                     RLRERAAYEFFFKSDAAGQPVWVADSAQAQPVFTDPNGVEPGAVVYVPGLKRFLLTCF
                     HVGPGQLGVFDAPHPWGPWTTIAYYERWGDMGSEGEGLTCGFPQKWMSADGLTLWSIF
                     SVYGDGAKQGINAHDRFNLVKATLQPFTPALPPASQ"
     gene            complement(3074..4849)
                     /locus_tag="VIL39_10595"
     CDS             complement(3074..4849)
                     /locus_tag="VIL39_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HEY5481920.1"
                     /translation="MMKASNDIHLTTVPRSTVEVHLPDGRIICGPRNRPIAAFLKGLP
                     EWNNHDPIMGAVINGELRELTHPVELDSRVRLVKMSDPDGSVIYRRSITFLLEAAFED
                     TFPTADISIDHSLSSGGFYCSVINRGPLSREELAAVEARMKELVKANLPFERSVVPLQ
                     DAISYFQAKGQAEKVQLLQFRSKDTLVLYKLRDRRDYHHGYMVPSTGYMHWFALSPMD
                     NGFALRFPRRQAPNELRPLPDSKKLLATFREYNSWLVRLGIENVGALDTAIQARRVSE
                     LILISEALHEHRIAEIASFVMERAKESRIVLVAGPSSSGKTTFSKRLAVQLLAQGISP
                     FPLELDHYFLDREHTPKDENGAYEFESLEALDTARLQADLQHLIAGEEIQLPRFNFPL
                     GRSEPGQVVKLEKDQLVILEGIHGLNPRLLPGLTQRQTTRIYVSCLTQLNLDRHNRIS
                     TTDTRLLRRIIRDARDRGYTAQQTISHWQAVGRGEKKHIFPYQENADEMFNSALVYEL
                     SAIRMLAEPLLRQVVFGTPEYIEAKRLLSFLQWFLPIDADLIPDNSLLREFIGGSILK
                     GFTLWQNGKNNGDYGSPGVLSELNC"
BASE COUNT          963 a         1448 c         1724 g         1086 t
ORIGIN      
        1 tcgagtatgc tggggccgct taccatgtga tggcctgcgg caaccaagga cgcgacatct
       61 atgtgggcgg gacagaattc aaggagcgga tgttggcgtt ggtggagcag ccgctgcgtc
      121 aggggcgaag cgactcctat agcggggagg ccaaacgggc gcacggagag gcggaagccg
      181 agcggttgct ggcgcgcgtt cccgtttgtc ttttacttct tcatgtccgg gtttcccggg
      241 ccaaagtgct tgagcatgac gatcggatcg gtcttcgagg gattggagat caccactcct
      301 gcgcgcgccg cgtcttcggt gatgaagaac tcgtccatcg tgagctggcc gtagcggatg
      361 atcgcagggg tgtcaatatc ccacacgccc atcttgccat ggccctggag caggatgatg
      421 ccgtaagccc cgccatcttt gatcgtgacg gtcttccctg ggagcacggt gagttccttg
      481 gcgctgtagt cctcggcgtc gtaggtaatc cagttctcct ggcagccctc agcgcgcatt
      541 tgttcgatcg agcggacggg cttcggacga cggaagtgat gggccgcgaa gtttggatcg
      601 gtgttcgcct cccaatccac gacctcgatc aggtgatcaa tgttgccgac cttgtccggc
      661 ggggtgttct tccataggag ttcctcgggg atgatggcgt cgccggtcag cgactggtac
      721 atcgcgaaga cgtcggaggc cttctgcggc tcgtaggtgc acaggcttcc cggagcgtgg
      781 aggatccccg cctggacatt ccagccggtt cccggttcca gccggtaggc ggccgacaag
      841 ttggtgatct tgttgtcgcc cttgttgaag ttctgcaggc attcgcgcag ttggtctttt
      901 gtggtgcccg gctgcaggcc gaagaacgtg tagggaaaat ccgccccgta gttgttgagc
      961 tggaccggga agaaatacga ttccggcttg cccatttggt tcacgagctt cgcgtgttgc
     1021 tctcggtgat ggatgtggtg cgggagcgga cccttgttgt cgaagaactt ggagtacatg
     1081 ggccagcagt ggtacgcgtc ccagaggcgc gcgccgatga tggacccctt cagctcgctg
     1141 accgcgtccc gcagggtcac tttctccgtc tgcgaaccgt cttccacgac gatgaagctc
     1201 aggccctcgt tcgggcttgt ctttgggccg ttgtcagccg gcgtggtcga agagaaccac
     1261 cgctcatcaa tgcctccgcg gtcgccgccc agcgcgtaat aatcggcggg atggagcttg
     1321 atccttctgc caggaacgca gaaggagcgc ggcacccagt tgggcgccag gcgtaatatg
     1381 ccttttccct gttcaatggc cttttctgca atcgagcgta cggacataac atttcacctt
     1441 tctgttgctt gctcccgggg agcgtgtttc acggatagag cgagttgatt tgtggtctca
     1501 ctcatcgcgg cagcaaggtg ccaagcgcga gaggtgatgt caaagggcga ctttgacacc
     1561 ccagcaacac gttttgtgga cctatacagg gtgaagtggt agagaatgcc cctattatga
     1621 agagcgcgct tttggcctcg ctggttttgg tgtcggttgt ggttggcgct tcggcttcaa
     1681 ccgagcctcc gtatccacac agccaggtgc tccaaagcat tgcctggcat tgggaaactt
     1741 ataccaccgc agcgttgggg agcgatctat ggccggtggc ctgggggccc gatgatcagc
     1801 tttacaccgc ctggggcgac ggtggcggat tcggcggcgg ggataaggac gggcgcgtag
     1861 ccctcggctt cgcccgcatc gaaggcacgc cggaacattg gcgcggtgtc aacgtgaatg
     1921 gcgggaagaa cccggagcac ccggcctctt ttccgcgcaa gggcaagacg agtgggcttg
     1981 cgatcgtgga tggcatcttt tatgccaccg tgaatctgca agacggcacc tggccgaatg
     2041 taaaccatgt gctggcctgg tccaccaact acggagccac ctggagcaaa gccgagtggt
     2101 tgtttgccag gggcgaaggg agttttcagc cggcgacgtt tctcaccttt ggaagagatt
     2161 acaccggcgc gcctgggcca ctggcgggct acgtctatat ttacggaccg aagctatccg
     2221 cggaccgcgg cagcggccaa tggctctatt tggcccgggt acccaggaac cggctgcgtg
     2281 agcgcgcggc gtacgagttc tttttcaaaa gcgatgctgc cgggcaaccg gtttgggtag
     2341 ccgacagcgc ccaggcgcag ccggtcttta ccgaccccaa cggtgtggag ccgggagcgg
     2401 tggtctatgt tccaggatta aagcgctttc tgctgacgtg ctttcatgtt gggccgggtc
     2461 aacttggcgt attcgacgcg ccccatcctt ggggaccctg gaccaccatc gcctattacg
     2521 agcgctgggg tgacatgggg tctgagggcg aagggctgac gtgcgggttt ccgcagaaat
     2581 ggatgagcgc cgacggcttg acgctctgga gcattttcag cgtgtatggc gacggcgcaa
     2641 aacagggcat taacgcccac gacagattca atctcgttaa ggccacgctt cagcctttca
     2701 ccccagccct cccgcccgcg tcccaatgat ggtcttctcc acgccgccgc cccgcgcgtc
     2761 agcacgttcg gattccaatt ttaccccagc accgaccccg tccggagcgt cggcgacggg
     2821 tcatctgaat taacgcagag acgcagagga acccagcgcg gcagagtcgc aatgaggccg
     2881 gaacaactga cagaaaaata gcgggacaga aaaatgggat gcccgtttat ctttatgtcc
     2941 ctcatttttc tgtcagcaga tattccattg ggtttcctcg cagcctgcga gcaatctcga
     3001 ctattgcagt acagagaaaa agtactctct gcgaatctct gcgttgctct gcgcctctgc
     3061 gttgaatctg tgctcagcag ttcaactcgg acaggacgcc gggggagccg tagtcgccat
     3121 tgttcttgcc gttctgccag agggtgaagc ccttgaggat cgagccgccg atgaactcgc
     3181 gcaggagcga gttgtcgggg ataaggtctg cgtcaatggg caggaaccat tgcaggaagg
     3241 agagcaggcg cttggcctcg atgtattccg gggtgccgaa cactacctgg cgcaagagcg
     3301 gctcggccag catccgaatg gccgacagtt catacaccag ggccgagttg aacatctcat
     3361 ccgcgttctc ctggtagggg aagatgtgct ttttctcgcc gcgcccgact gcttgccagt
     3421 ggctgatggt ctgctgggcg gtgtagccgc ggtcacgggc gtcgcggatg atgcgccgga
     3481 gcaggcgggt gtcggtggtg gagatgcggt tgtgccggtc gaggttcaac tgggtcaggc
     3541 aggagacata gatgcgtgtg gtctggcgct gggtgaggcc gggcagcagg cgcgggttca
     3601 ggccgtggat gccttccagg atgacgagct ggtccttttc gagcttcacg acctggccgg
     3661 gctcgctgcg gcccaggggg aaattgaacc tgggcaactg gatttcctcc ccggcaatca
     3721 agtgctgcag gtcggcctgc aagcgggcgg tgtccagcgc ctcgagggat tcaaactcgt
     3781 aggcgccgtt ctcgtccttg ggagtgtgct cgcggtcgag gaagtaatgg tccagctcaa
     3841 gcgggaaggg agaaatgccc tgggcgagga gctggacggc gaggcgcttg gagaaggtgg
     3901 tcttgccgga ggatgagggg ccggccacca gcacgatgcg agactccttg gcgcgctcca
     3961 tcacgaagct ggcgatttcg gcgatgcggt gctcgtggag ggcttcggag atcaggatca
     4021 attcgctaac gcgcctggcc tgaatggcgg tgtccaacgc gcccacgttt tcgatcccca
     4081 ggcgcacgag ccaactgttg tattcgcgaa aggtggccag cagcttcttg gaatcaggca
     4141 gtggccgcag ctcgttcggc gcctggcggc gggggaagcg cagcgcaaaa ccgttgtcca
     4201 tggggctcag ggcgaaccag tgcatatagc cggtggatgg gaccatgtag ccatggtggt
     4261 aatcgcgacg gtcgcggagc ttgtagagca ccagggtgtc cttgctgcgg aactggagca
     4321 gttggacctt ctcggcttgg cccttcgctt ggaaatagga aatggcgtcc tgcagcggca
     4381 cgacgctgcg ctcgaagggg aggttggcct tgaccagctc cttcatgcga gcttcgacgg
     4441 ccgccagctc ttccctggac agggggccgc ggttgatgac cgagcagtag aaaccgcccg
     4501 aggagaggga gtgatcaatg gagatgtccg ccgtggggaa ggtgtcctcg aaggcggctt
     4561 cgagcaggaa cgtgatcgag cggcggtaga tgacggagcc gtccggatcg gacatcttga
     4621 cgagccggac acgggagtcc aactccaccg ggtgagtcag ttcacggagt tcgccgttga
     4681 tcaccgcgcc catgatgggg tcgtggttgt tccattccgg caggcctttt aggaaggcgg
     4741 cgatgggccg gttgcgcggt ccacagatga tccggccatc gggcaggtgc acttccacgg
     4801 tggatcgggg gacagtggtt agatggatat cgttcgaagc cttcatcatt gacctggatt
     4861 agcggagcga aggggaattt acccttattt cgcagtccgc agggaactga caaacggcat
     4921 ggcaaccggc gcgcaggcaa cccgtttgcc gcgtccacca gtagtattcg ctaaaatcac
     4981 gcatatatgg ataccgcaca ccctttgggg gagcaagtcg caagccaaga tcggacatgc
     5041 ggcgtcttga gcctgccgcc aagcccggcg cgcggacatt cctgtccgca gcagcttccg
     5101 gacgcttgtc cgtgctgtcc gcgcgcttgt tcacgtttaa cactgctgcg gacaggaatg
     5161 tccaatgccg ctaggatttg ctcccacaaa atgcgccaga ccggggcaaa tctttgtccc
     5221 g
//
LOCUS       DATCGS010000517         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_737002, whole genome shotgun sequence.
ACCESSION   DATCGS010000517 DATCGS010000000
VERSION     DATCGS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737002"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VIL39_10600"
     CDS             <1..1007
                     /locus_tag="VIL39_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481921.1"
                     /translation="KQLEKSLADFEQLHPRPFKGTEAEAQQTQDAMTKAAESLQKKNT
                     DAKSSTQRATQQLEKLSEAMKGASTGQQLADAYKLKQMLDKEIKTLGKCANPGAGGGA
                     SDADVDRTASEARETLNQLKKVAEQEPTRDAFGPPLRNALSGQNKVELDSKLARVQLA
                     QDQPTRQERAGEAKDALSKVSKAFEESEPKAMQMAQQSDSLKPGEQESFNQGMSELQG
                     LLKQLEDKRQMTRENQSKQGKEALGNLQRGVRGLYGNNDRATQLLLKLDQKLKGEKPL
                     DTEDLRQLINELQHFSVETADQMSKREDKPEVTNIDPTRLPPAYRGRIQKYFQRLSEK
                     "
     gene            1004..3232
                     /locus_tag="VIL39_10605"
     CDS             1004..3232
                     /locus_tag="VIL39_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481922.1"
                     /translation="MIAAIVFEFPKGWLFALPLGVALAFAIWRQHRRGLATSRIVGLG
                     ALRAVALLVLVFLAARPVWMTREPPASATRSVVLLMDRSESMSLEERDATRYQQALGF
                     LRERLLPALRAASLPVQAMLFDQGAEPADGAKLGAATPNGKRTNLGGAVGQALASAAQ
                     PPLAVIALTDGIVNESADNTHALTALVDACVPFIGVGFGSDQGVRTLSLREVAAPSIV
                     STKTAFNISAQLEMVNTEDMPGFELLLFRDGQMRQKRSVIPGKGSRTWLESFPVTEEK
                     QGVHNYAVQLLPPNVPGLKCVNMLANTSVRVSDEKELRVLYIQGALTWDYKFISLALR
                     NDQTIKLTGLTRTSKQSVFRQNVESAGELLHGFPTSLEELAPFRVVVLSNLRPTDLTS
                     AQQEVLARFCGELGGGVLMIGGPATFDSSWQSSRLEQLLPVVFSPNRGVQGLDRPFRL
                     ELAEDALQHPVFQIADNRAMREAWAQLPTFTHYGRVDAAKPGAQVWAVHQTDEGPQGR
                     RILMASQRYGAGLSAVVCIQNFWRWRLAKDSEPQQFDRFWRQLFRFLSEVGRQDVSIH
                     LADQELHPQMDVQVVLEKQPNPKNITDTSHKFFVRVEDGQKKLLHEGTVELESGRPVD
                     FRFHADKADIYTVTVSDAMKVPIATRPIEIRDLNVEFQETARNMETLQQWASVSDGLA
                     MKVEDCRDAADLVAQIKAKIEQVRHGKQMRRPVGVNGWLLALVLGCLGGEWLLRKRWG
                     LA"
BASE COUNT          694 a          962 c         1193 g          593 t
ORIGIN      
        1 aaaagcagtt ggagaagtcg ctggccgact tcgagcagct ccatccgcgc cccttcaagg
       61 ggacagaggc cgaggcgcag caaacccagg atgccatgac taaggcggcg gagtcgttgc
      121 agaagaagaa cactgacgcg aagtcctcca cgcaacgggc gactcagcag ttggagaaac
      181 tcagtgaggc gatgaagggc gcctcgactg gacagcagct tgccgatgct tacaagctga
      241 agcagatgct cgacaaagag atcaagacac tcggcaagtg cgccaaccca ggagcgggcg
      301 gcggggcgtc ggacgccgac gtggaccgca ccgccagcga agcgcgcgag acgctgaacc
      361 agcttaagaa ggttgccgag caggagccga cgcgggatgc gttcggcccg ccgctgcgga
      421 acgcgttgag cggccagaat aaggtggagc tggactcgaa gctggcgcgg gtgcaactgg
      481 cccaggatca gcccacccgg caggagcggg cgggtgaggc caaggatgcg ctgagcaagg
      541 tcagcaaggc cttcgaagaa agcgagccca aggcgatgca gatggcgcag cagtctgatt
      601 cgctgaaacc gggggaacag gagagtttca accagggcat gtccgagctg caaggcctgc
      661 tcaagcagct tgaggacaaa cgccagatga cccgcgagaa ccagagcaag caaggcaagg
      721 aagcgttggg gaacttgcaa cgcggtgtgc gtgggttgta cggcaacaat gatcgcgcga
      781 cccagctcct gctgaagctg gaccagaagc ttaagggcga gaagccgctg gacacggagg
      841 atctgaggca actgatcaac gagctgcagc acttctccgt cgagaccgcc gaccagatgt
      901 cgaagcggga agacaagcca gaggtgacca acattgatcc gacgcggctg ccgccggcct
      961 atcgcggccg tatccagaag tatttccaga ggctctccga gaaatgatcg ccgcgattgt
     1021 ctttgaattc cccaagggct ggctctttgc gttgccgctg ggcgtggcgc tggcgttcgc
     1081 tatctggcgg cagcaccggc gcgggctggc gacctcacgg atcgtcgggc tgggagcatt
     1141 acgcgcggtc gctttgctgg ttctcgtttt cctggccgca cggcctgttt ggatgacccg
     1201 ggaaccgccg gcgtcggcga cgcggtcagt ggtgttgctg atggatcgga gcgagagcat
     1261 gtcgctggag gaacgggacg caacgcgata tcagcaagcg ctcggatttc tgcgggaacg
     1321 attgttgccc gccttgcgag ctgccagcct gccggtgcaa gcgatgctgt tcgaccaggg
     1381 agccgagccg gcggacggag cgaaactggg ggcggcgacg ccgaacggga agcgcaccaa
     1441 tcttggcggg gcggtcgggc aggcgctggc cagcgcggct cagccgccgt tggccgtgat
     1501 tgcgctgacg gacggcattg tgaacgagag cgcggacaac actcacgcgc tgacggcgct
     1561 ggtggacgcg tgcgtgccct tcatcggggt gggattcggc agcgaccagg gagtgcggac
     1621 gctttccttg cgcgaggtgg cggcgccttc gatcgtctca accaagaccg cgttcaacat
     1681 ttcggcgcag cttgagatgg tcaacacgga agacatgccc ggcttcgagt tgctgctctt
     1741 ccgcgacggg cagatgcggc agaagcggag cgtgattccg ggcaaaggtt cgcgcacctg
     1801 gctggagagc ttcccggtga ccgaagagaa gcagggcgtc cacaactacg cggtgcaatt
     1861 gctgccgccg aatgtgccgg gcctgaaatg cgtgaatatg ctcgcgaaca cctcggtccg
     1921 cgtctcggac gagaaagagt tgcgcgtgct ctacattcaa ggggcgctga cctgggacta
     1981 caagtttatc agcttggcgc tgcgcaacga ccagacgatc aagctcaccg gcctgacgcg
     2041 cacgtcgaag cagtctgtgt ttcgccagaa cgtggagagc gcgggcgagt tgctgcatgg
     2101 gtttcccacg tcgctcgaag agcttgcgcc cttccgggtg gtggtgctgt cgaatttgcg
     2161 cccgacggac ctgacgtccg cgcagcagga agtgctggcg cggttttgcg gcgagctggg
     2221 cggcggagtt ctgatgattg gcgggccggc cacgttcgac agttcgtggc agagcagccg
     2281 gctggaacag ttgttgccgg tggtgttttc ccccaaccgt ggagtgcaag ggctggaccg
     2341 gccattccgc ctggagcttg cggaggatgc tttgcagcat ccggtctttc aaatcgcgga
     2401 taatcgcgcc atgagggagg cgtgggcgca gttgccgacc ttcacgcatt atgggcgcgt
     2461 ggatgccgcc aagcccggcg cgcaggtgtg ggcggtgcac caaacggacg aaggaccgca
     2521 gggccgccgc atcctgatgg cttcgcagcg ttatggcgcc ggattgtcgg cggtggtgtg
     2581 catccagaat ttctggcgct ggcggctggc gaaggattcg gagccacagc agtttgaccg
     2641 cttctggcgg cagctttttc gcttccttag cgaggtcggc cggcaggatg tctcgatcca
     2701 cctggctgac caggaattgc atccgcaaat ggacgtgcaa gtggttctgg agaagcagcc
     2761 caacccgaag aacatcaccg acacgagcca caagttcttt gtgcgcgtcg aggatggcca
     2821 gaagaagctc ctgcacgagg ggaccgtgga gttggagtca gggcgcccgg tggactttag
     2881 gttccacgcg gacaaggctg acatctatac ggtcacggtc tccgacgcga tgaaggtgcc
     2941 gattgcgacg cggccgatcg agattcggga cctcaatgtg gagttccaag agacggcccg
     3001 caacatggaa accctccagc agtgggcgtc agtcagcgac ggcctggcga tgaaggtcga
     3061 agactgccgc gatgcggctg atttggtggc gcagatcaag gccaagatcg aacaggttcg
     3121 ccacggcaag caaatgcgcc ggcctgtggg cgtcaatggc tggctcctgg cgctggtgtt
     3181 gggctgtctg ggcggcgagt ggctgctgcg caaacgatgg gggttggcct agtggaagga
     3241 gttgcatggg ttacattcgt tacaaggtta aatgaggaaa cgatcatgga cataatgaat
     3301 caccgagccc gcagcttcca ctccttcacc cattgcgtgt cgctggtcac gcttctcgct
     3361 ctcctgaatc tggccgccgc tttcgggcaa acgacagacg acaagcaagc caccttcttc
     3421 atgggccgcg tgaagtacag cg
//
LOCUS       DATCGS010000518         6107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_42104, whole genome shotgun sequence.
ACCESSION   DATCGS010000518 DATCGS010000000
VERSION     DATCGS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42104"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1510
                     /locus_tag="VIL39_10610"
     CDS             170..1510
                     /locus_tag="VIL39_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481923.1"
                     /translation="MQHPSPGTKVNRRQFLGLTGAALAFPTLISSSVLGRAGPPAPSK
                     RITLGIVGCGVMGTGNTNSFLALKDCQVVAACDVDRKHLETLVRKVNGHYKDTGCKAY
                     HDYRELLARRDIDAVMLAVPDHWHALAAVEAARQKKDIYGEKPLARTIAEQQAIVHAV
                     QVNGRIWQTGSWQRSVAVFHKAAEIVRNGLIGKVTRVEVGLPAGHGSRVQAAADMLPS
                     TPPPELDYETWVGPSKLTPYIRCQIHREWRWNYNTGGGQLLDWIGHHCDIAHWGCDFD
                     NSGPLEIEGHGEFPPVDAVWNTCTKYRIELKYPRDITMIIAGGYPDIRGGTKWIGTDG
                     WVWVDRGVFEASNEEWRDYRRLPDELRKVKLPISDNHQRNFLDSVKSRKPTITPAETA
                     HHSAIPGHLGLISMLVGRRLKWDAQNERILDDADASKLLTRNYRAPWKLAGYAG"
     gene            complement(1507..2757)
                     /locus_tag="VIL39_10615"
     CDS             complement(1507..2757)
                     /locus_tag="VIL39_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481924.1"
                     /translation="MKSDNQSGSADRPASEKPLSISSITRRSFLHAVGLGSAALALTP
                     TARSQEKVIQGFEQTPTAPDASKGWKPVSDRKIRVGLVGYGVCQFAAAFSFQNHPNVE
                     VVAVSDLVPDRCAELSKVTRCAKTYPSLEELVKDDRIEAVFVATDAPSHARHCIEVLK
                     HGKHVASAVPAVFGSVEDAHKLFDAVKASGLKYMMFETSYFHEDLYGMRQIYNAGGLG
                     KLLYSEGEYFHYMSTPIDSYKGWRIGLPPQWYPTHSNAYYVGVTGGSFTEVSCLGMPS
                     KIDQFQPANNRYKNPLATEIALFRTSEGGMSRMGVSWDSPGFGGEMGRIRGQKGSYYG
                     NYDGLEKNLPDIKRPPLPPGVDAGGHGGSHGYLMNEFVTAILQNRKPLVDIAQALNMT
                     VGGIVAHQSALKGGELTKIPQFKL"
     gene            2983..3891
                     /locus_tag="VIL39_10620"
     CDS             2983..3891
                     /locus_tag="VIL39_10620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5481925.1"
                     /translation="MKINDLSRRQFLERSAGAAGALLLTSSAWFNAQAADKRTATDQV
                     ALGKTGLKLSRLGFGTGSNSGNVQYALGQEEFNKLIHYAYDQGITYFDCAQSYRTFEW
                     IKGAIKGLPREKLFIQSKVMGKPDEVLAAIDHHRKVFDTDYVDSLLIHCMVKDGWTDE
                     WKRIMDAFDEAKEKKWIRAKGVSCHSLPALRTATASNWTEVHLVRVNPQAKHIDGMDP
                     SWDKSGTDMTPVLAELKTMRAKGRGIIGMKIIGNGDFTQAEDREKSIRFAMSRPELDA
                     IVIGFKSRAEIDEAIRRVNQALAETA"
     gene            4152..4820
                     /locus_tag="VIL39_10625"
     CDS             4152..4820
                     /locus_tag="VIL39_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY5481926.1"
                     /translation="MQCIITAGPTYEPLDEVRRLTNFSTGRLGSELVNHLAARGHEVT
                     LLIGQHATYRGERYARRVETFTTTANLRERLQALASPAVAAVFHVAAVSDFAFGKIWR
                     RSPQGELTECKGGKISTREGTLLAELVPTPKIIAELRQWFPTARLVGWKYEVEGGRAG
                     VIRLAERQVAECRTDACVANGPAYGTGFGLVRGGGEVTHLPDAATLFVALEEFMCERQ
                     EAQR"
     gene            4909..5688
                     /locus_tag="VIL39_10630"
     CDS             4909..5688
                     /locus_tag="VIL39_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5481927.1"
                     /translation="MKAGKFSRRRFLAVALLSAPALAVADARWLEPTWVKVRQVRLGN
                     RRPTHRVVHFTDVHHKGDRVYLHTVVRQINALSPDFVCFTGDLIEETRHLAEALEAFA
                     GIKSPVYGVPGNHDYWSRAPFDGLAKCFAATGGAWLLDQQQVTRDGKFSIIGATCLSS
                     NQPPIHASSATRNIFLMHYPAWVKKLGGQQFDLLLAGHSHGGQVRLPFYGPIIVPFGV
                     DQYDRGLFHTDAGPLYVNPGIGWFPVPIRFNCRPEITVFDI"
     gene            complement(5781..>6107)
                     /locus_tag="VIL39_10635"
     CDS             complement(5781..>6107)
                     /locus_tag="VIL39_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5481928.1"
                     /translation="VMNIMLVSVTERTREIGIRRAVGAKKRNIMTQFIIEAIVLCEIG
                     GVIGVLCGILGGNSTAWFFKLDPVIPIDWMVLGLFVCSVIGVIFGTYPAFKAANLDPI
                     ESLRYE"
BASE COUNT         1226 a         1851 c         1906 g         1124 t
ORIGIN      
        1 cgtggatttg aatcccgtgc tgcatgggcg cggagccgcc gacggcctgg atgttgaggc
       61 cggcgacgag tgagaccagc ccggccagga ggaatctgcg gcggccgctc gggcgtggag
      121 tatctgttgt gttcatgaat acatacgatt actgtctcgt cctgcgacta tgcaacaccc
      181 atcaccgggc acaaaagtca accgccgcca gtttctgggc ctgaccgggg ccgcgctcgc
      241 tttccccacg ctcatctcca gcagtgtgct ggggcgggcc ggcccgccgg ctccctccaa
      301 gcgcattacc ctgggcatag tcgggtgcgg ggtcatggga accggcaata ccaactcctt
      361 cctcgccctg aaagactgcc aggtggtcgc cgcctgcgac gtggaccgga agcacctgga
      421 gacgcttgtg cgcaaggtca acggtcatta caaggacacc ggctgcaagg cttaccacga
      481 ttaccgcgag ctgctggccc gccgcgatat tgatgcggtc atgctggcgg tgccggacca
      541 ttggcacgcc ttggcggcag tcgaggcagc gcggcagaag aaggacattt acggcgagaa
      601 gccgctcgcc cgcacgatcg ccgaacagca ggcgattgtc catgccgtgc aggtcaacgg
      661 gcgcatctgg caaaccggtt cctggcagcg ctccgtcgcc gtgttccaca aagcggcgga
      721 gatcgtgcgc aacggcctga tcgggaaggt tacgcgggtt gaggtgggcc tgcctgccgg
      781 acatggcagc agggtccagg ccgccgcgga catgctcccc agcacgccgc cgcccgagct
      841 ggattacgaa acctgggttg ggccgtcaaa gttgacgcct tacatccgct gccagattca
      901 ccgggaatgg cgctggaact acaataccgg cggcggccag ttgctcgact ggatcgggca
      961 ccattgcgac atcgcccact gggggtgcga ttttgacaac tccggcccgc tggaaattga
     1021 aggccacggc gagttcccac cggtggacgc cgtctggaac acctgcacca aataccgcat
     1081 cgagctgaag tatccgcgcg acatcacgat gatcatcgct ggcgggtacc ccgacattcg
     1141 cggcggcacc aaatggattg ggaccgacgg ctgggtgtgg gtggaccgcg gggtgttcga
     1201 ggcctccaac gaggaatggc gtgattacag gcgcctgccg gacgagctgc gcaaggtaaa
     1261 gctgcccatc tcggacaatc accagcgcaa cttcctggac tcggtcaagt cgcgcaagcc
     1321 gaccatcacg ccagccgaga cagcccacca ttcggcgata cccggccatc tgggcctgat
     1381 ctcaatgctc gtcggccgga ggcttaagtg ggatgcgcag aatgagcgca tcctcgatga
     1441 tgcggacgcc agcaagctac ttacccggaa ctaccgcgcg ccgtggaagc tcgccgggta
     1501 tgcgggctag agcttgaact gcgggatctt cgtcagctcg ccgcccttca gggcggactg
     1561 gtgggcgacg atgcccccga cggtcatgtt cagggcttgg gcaatgtcca ccagcggctt
     1621 gcggttctgg aggatggccg tgacgaactc attcatcagg tagccatgcg acccgccgtg
     1681 gccgccggcg tccacaccgg gaggcagggg cggacgcttg atgtcgggca ggttcttctc
     1741 cagcccgtcg taattgccat agtaggagcc tttctgcccg cgaatccgtc ccatctcccc
     1801 gccaaagccg ggagagtccc aactgacgcc catccgcgac atgccgcctt cgctggtgcg
     1861 aaacagggcg atctcagtgg ctaagggatt cttgtaacgg ttgttggccg gctggaattg
     1921 gtcaatctta ctcggcatgc ccaggcagga cacttcggtg aagctgcctc cggtgacgcc
     1981 gacgtagtag gcgttggagt gcgtcggata ccattgcggc ggcagtccga tgcgccagcc
     2041 cttgtacgaa tcaatggggg tggacatata gtggaaatac tccccctcgg aatacaggag
     2101 cttgcctagc ccgccggcgt tataaatctg acgcattcca tagaggtcct cgtggaagta
     2161 tgatgtctcg aacatcatgt acttgaggcc gctggccttg acggcgtcga acagtttgtg
     2221 ggcatcttcc accgagccaa acaccgccgg cacggccgag gcgacgtgtt tgccgtgctt
     2281 gaggacctcg atgcagtgcc gcgcgtggct gggagcgtcg gttgccacga agacggcttc
     2341 gatacgatcg tccttgacca gttcttcgag cgagggatac gtcttagcgc accgggtgac
     2401 ctttgacagt tccgcgcacc gatcaggaac cagatcgctg accgccacca cctcgacgtt
     2461 cgggtggttc tggaagctga acgcagccgc gaactggcaa acaccgtagc cgaccagccc
     2521 tacccgaatc ttccggtcgg agacgggctt ccagcctttg gaagcatccg gggcggtggg
     2581 cgtctgctcg aatccctgaa tgaccttttc ctgggatctg gctgtcggcg tgagcgccag
     2641 agccgcagag cccaggccca ctgcgtgcag gaacgagcgt cgcgtaattg aactgatgga
     2701 gagaggtttc tctgaagccg gacgatccgc agagccagac tggttgtcgc ttttcatgac
     2761 ccatgagcat ctccaacacc gaactcgcgt caagaccgaa ccagccggga ctggcagaaa
     2821 acccagggcg ggtgacaagg accagcctaa agcgctgggg aaggggccgt ggcagaaaac
     2881 ctgactgctc attgacgggc tttctggcgg tgttagacta tgagcgacga tgcaagcgca
     2941 tgatgcagtt gtcttttgaa agccggaccc gcgaagaaca ttatgaagat caatgacctg
     3001 agccgccgcc agtttctcga acgctccgcc ggtgccgccg gcgcgctgct gctaacctcc
     3061 tcagcgtggt ttaatgccca ggctgccgac aagcgcaccg ccacggacca ggtcgctctc
     3121 ggcaagaccg gccttaaact gagccgcttg ggtttcggca ccggcagcaa cagcggcaac
     3181 gtccaatacg ccctgggcca ggaggagttc aacaagctca ttcattacgc ttacgaccag
     3241 ggcatcacct actttgactg tgcgcaaagc taccgcacct tcgagtggat aaaaggcgcc
     3301 attaagggcc tgcctcgtga gaagctgttc atccagtcca aggtcatggg caaacccgat
     3361 gaggtgctcg cggccatcga ccaccaccgc aaggtttttg acacggatta cgtggatagc
     3421 ctgctgattc attgcatggt caaggacggc tggaccgacg agtggaagcg catcatggac
     3481 gccttcgacg aggctaagga gaagaagtgg attcgagcca agggcgtctc ctgccacagc
     3541 ctgcccgctt tgcgcactgc gacggcctcc aactggaccg aggtgcacct ggtgcgggtg
     3601 aacccgcaag ccaagcacat cgacggcatg gatccgagct gggacaagtc cggcaccgac
     3661 atgacgcccg tgctggcgga actcaaaacc atgcgcgcca aaggccgggg catcatcggc
     3721 atgaagatca tcggcaacgg cgacttcacc caggcggagg accgcgagaa atccatccgc
     3781 ttcgccatga gccggcccga gctggacgct atcgtcatcg gtttcaagag cagggcggaa
     3841 attgacgaag ccatccggcg ggtgaaccag gcgttggctg agacggcttg agatagacat
     3901 tgtcaccgcc cctggccggc atgactcaag aaccgccttg ctgatttcgg gccgggaact
     3961 agtatgcctt catgcaaccc cactcgcgac ggggtaggaa gcggcgacga ttcaatcgca
     4021 attacgggac gcttatcgtt gtggggctgt gcacggtcgt ggcggtcgcc ttcgtcgctc
     4081 ttctcatgta cctgctcacg agcatggagt ggcgctggcg tcattagcac caggcggaaa
     4141 gatctccatt tatgcagtgc attattacgg ccggccccac ttatgaaccg ctcgatgagg
     4201 tgcggcggct caccaacttc tccaccggcc ggctcggttc ggagctggtc aatcacctgg
     4261 cggcgcgtgg gcatgaggtg accttgctga tcggccagca cgccacgtat cgcggggagc
     4321 gttacgcccg gcgggtggaa acctttacca ccacggcgaa tctgcgcgag cgcctgcaag
     4381 cgctggccag tccggccgtg gcggcggtat tccacgtggc ggccgtgagt gattttgcct
     4441 tcggcaagat ctggcgccgt tccccgcagg gcgaattgac cgaatgcaag ggcggcaaga
     4501 tctccacccg cgagggaacg ttgctggcag aactggtgcc gacaccgaag atcatcgcgg
     4561 aactgcgcca atggtttcca acggcgcggc tcgtgggctg gaagtatgaa gtggaaggtg
     4621 gtcgtgccgg ggtgattcgc ctggcagaac ggcaagtcgc cgaatgccgc acggatgcct
     4681 gcgtggccaa tggaccagct tatggcacag gtttcggtct ggtgagaggc ggcggcgaag
     4741 tcacgcacct gccggacgcg gcgactctgt tcgtggcatt ggaggagttc atgtgcgaac
     4801 gccaagaggc ccagagatga cccgcggaac ccgcgcatcc aagcgaagta actcctgcca
     4861 cgggagcatt gcttacccaa gagcgcaatc cagaatggaa agccacccat gaaggccggc
     4921 aagttcagtc gtcgccgatt cctggcagta gctttgctgt cagcgccagc cctggcggtg
     4981 gccgatgcaa gatggctgga accaacatgg gtcaaggtcc ggcaggtccg cctcggcaac
     5041 cgcagaccga cacaccgggt cgtccacttt accgacgtgc atcacaaggg cgaccgcgtc
     5101 tatttgcata cggtcgtgag gcagataaac gcgttatcac cggactttgt ctgtttcacg
     5161 ggcgacctga tcgaggaaac aaggcatctc gccgaagccc tggaagcctt tgccggtatt
     5221 aagtcgccgg tgtatggcgt gccgggcaac cacgattact ggagccgggc gcctttcgat
     5281 ggccttgcca agtgtttcgc cgccacgggt ggtgcgtggt tgctcgacca gcaacaagtg
     5341 accagagatg ggaagttctc catcatcggc gccacttgcc tgagttccaa ccagccgccc
     5401 atccacgcca gctccgccac ccggaatatc ttcctcatgc actacccggc ctgggttaag
     5461 aaactgggcg ggcagcaatt tgatctgcta ttggccggcc attcgcatgg tgggcaggtg
     5521 cgcctcccat tctacggacc gatcatcgtg ccgtttggtg tggaccagta cgacagaggc
     5581 ttgttccaca cggacgccgg gccgctctac gtcaatcccg gcatcggttg gttcccggtg
     5641 cccatccggt tcaactgccg cccggaaatc acagtattcg acatctaatg gaggcctaac
     5701 ggcaccagcc cattgtgatg attgatgggc ccgccactga cgggcagcgg ttctacaggg
     5761 cgttcctggg attctaaacc ctattcatac cgcagcgatt cgatcgggtc gaggttcgcc
     5821 gctttgaaag cggggtaggt tccaaaaatg acgcctatca ctgagcagac gaacagtccg
     5881 aggaccatcc agtcgatggg aatgaccggg tcgagcttga aaaaccaggc ggtcgagttc
     5941 ccgccgagga tgccgcacag gacgccaatg accccgccga tctcgcagag cacgatggcc
     6001 tcgatgatga actgggtcat gatattccgc ttcttggcgc cgaccgcgcg gcgaatcccg
     6061 atctcccgtg tgcgctcggt gaccgagacg agcatgatgt tcatcac
//
LOCUS       DATCGS010000519         6254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_490260, whole genome shotgun sequence.
ACCESSION   DATCGS010000519 DATCGS010000000
VERSION     DATCGS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490260"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..417
                     /locus_tag="VIL39_10640"
     CDS             262..417
                     /locus_tag="VIL39_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481929.1"
                     /translation="MTLNTMFSRLNLTVAALLLLLAGAVLLAGCESMGDNDTSAHDHS
                     SGQGSCH"
     gene            441..1862
                     /locus_tag="VIL39_10645"
     CDS             441..1862
                     /locus_tag="VIL39_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5481930.1"
                     /translation="MNSKPQRSRFHLIIVLSALAAAAVLLPGCKGIPTKSERQSRADL
                     KAVTQTYRPDGQKPVLLPLMATSSLSNYLAYALLNQPKVEAAYYDWAASIERITTARS
                     LPDPQFTFQMDIQNIVSSIMPGLIGTIPWPDKLRVGASIASAESRAKYFAFQSAVLES
                     AFEVKRAYYQLYFLEEKIRVNQETLRLLADLERLARAQNEVGKVTLQDVLRAQIEQDR
                     LKTDIANLDDSRRSLLAQFKAALGLGADDPTPPIPGRFESTPLDLTADKLFETALAQN
                     TRLKAMEADVRAAEATIILARKARLPDSTLGLMADVKMDPTLYRPLGTVSLPIWRDKI
                     AAQIAEAQANQRSAEARLTGEQIALAVDFAERSFLYREATRNLALLNDKLLPKARRSL
                     EVARSGYLAGQIDFFNLTDTERTLLGFKLDKVEATTQREVVLAELSLIIQGMPAANAS
                     MASKTSGMPRGKPSASKKMGGGM"
     gene            1888..3882
                     /locus_tag="VIL39_10650"
     CDS             1888..3882
                     /locus_tag="VIL39_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5481931.1"
                     /translation="MKFHALSAKSTVLFMLAFLIAAGAGLLLSGCGRAQAPAANADAA
                     KTLYTCGMHPQVVQPTPGNCPICGMKLTPIRKQMEAAKGSSATPSGERKIKYYKSTMT
                     PGEVRPTPGKDTMGMEMVPVYEDEATAAASQTIAIDPVTTQNMGIRTAVVTRGPLRRT
                     IRTVGAIDYNETALEDVTTKFKGWIEKLYVDATGQQVHRGDPLFEIYSPELYSAQREY
                     LLAIEDGTNAADGASLKTSALTKLRFLDISAGQIAEMERTGQPTKTLRILAPMDGVVV
                     EKMVVQGQMVDPGMKLYRLADLGLVWVQAQIYEQDLAYLKLGQEATVTLSYLPDREFR
                     GRVTYIYPNVDEKTRTARVRMEFHNPGYFLKPGMFATVQVSSELEASVLLAPDMAILR
                     SGEKNTVFVALEEGKFEPRTIVLGPQADNDMYQIISGLKEGERIVTSGQFMLDSESQL
                     REAIQKMLEPKQASQPQSAVTPQNPPDTAAHAGHESMTMPAGTLSEEAKAVKYVCPMP
                     EHISIQYDHPGKCPICAMTLVPVSGATLKKLQPGGKLLYYTCPMPEHSDVRSDKPGKC
                     PKCAMTLIPVLQAPPISNTATNQASADKTQALPTTLYTCPMASHADVVSDKPGKCPKC
                     EMELVPTSTVSHGKIAEENWRKQHSGAPPSDVTMPPQRQH"
     gene            3894..>6254
                     /locus_tag="VIL39_10655"
     CDS             3894..>6254
                     /locus_tag="VIL39_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5481932.1"
                     /translation="MLKDIIDFSLKNKFIVLLGTIALVLGGLYALKNIPLDAIPDLSD
                     VQVIVYTEWPGQAPQIVQDQVTYPITTKMLSVPKAKVVRGYSFYGFSFVYVIFEDGTD
                     PYWARTRVLEYLSGMSGRLPAGVSPSLGPDATGVGWAFMYSITSTNRDLAELRSMQDW
                     YLRYQLASVEGVSEVASVGGFVKQYQITVDPTKLRAYNLSISEVAMAVKRSNGEVGGR
                     SIELSEKEFMLQVRGYIQSLEDLRKIAVGVGDKGVPILLRQVANVEFGPDMRRGIAEL
                     NGEGETVGGVVVVRYGANARQVILDTKKRLDQAMKGLPADVQLTVVYDRTALINRAVK
                     TLEGKLIEESIVVALVCLAFLMHLRSAFVAILILPIAVLMSFLVMFGQGISSNIMSLG
                     GIAIAIGAMVDAVIIMIENAHKHMEHDQGKKPHWDIIRDAAIEVGPTLFYSLLVITVS
                     FLPVFTLQAQEGRMFKPLAFTKTYSMAAAAILSITLAPVLMGLFIRGKIPAEEKNPLN
                     RLLVWLYHPMIDFVIKWRWPVIFTAAGLIVWVFFPWNSLAARLLPDGKAKEWAFKVGK
                     FFPYQSLGSEFMPPLYEGDLLYMPTTFPGISPTKAREILQTTDKIIRRFPEVHHVFGK
                     VGRAESATDPAPFDMIETTIMLKPEAEWPVVDIKDDAGKIIAHRRRTPDELTDVMNAA
                     VQIPGLNNAWTMPIKTRTDMLATGIKTPVGIKVAGPDLAELERVASEIEAVVKGVPGT
                     LSAFAERTMGGNYIQFNIDRDAIARYGLTIGDVQDVLEVALGGMPLT"
BASE COUNT         1304 a         2011 c         1735 g         1204 t
ORIGIN      
        1 tgccggggtc gcgatgagtt tcagttcggt gtcggtcatt tttaacgcgc tacgactgcg
       61 caaggtgaat ctgtaacctc gccgctcaag ccacgcctaa aagttttctg agggatttcc
      121 tcttctgtgg cgtaagcaat agtgtggggc gcggttccga ggcgctctca ggaaacaatt
      181 ggcagaactg cggtcccgtt gccaaccgac ggacaacgga accgcaacga cgacaacttt
      241 aagaagacag acatagatgc tatgacacta aacacgatgt tttcgcggct gaacctcact
      301 gtggccgcct tgctgctcct cttggccggc gcggttctac ttgccggctg cgagagcatg
      361 ggtgacaatg acaccagcgc ccacgaccat tccagcggcc agggttcctg ccactgacgg
      421 cgcgtcggcg tttggtgaat atgaattcca aaccacaacg ttcccgcttc cacttgataa
      481 ttgtgctgtc cgccttggcg gcggcggccg tcttgctgcc cggctgcaag ggcattccca
      541 cgaaaagcga gcgccagtca cgggcggacc tgaaagcggt cactcaaacc tatcgtcccg
      601 acgggcaaaa gcctgtgtta ctgcccctca tggcgacctc cagtctcagc aattacctcg
      661 cttacgcact gctcaaccag ccgaaggtcg aagccgccta ctacgactgg gctgcctcga
      721 tcgagcgcat tactaccgcc cgctccctgc ccgaccccca gttcaccttc cagatggaca
      781 ttcagaacat cgtcagttcg atcatgcctg ggcttattgg gaccatcccc tggccggaca
      841 aactccgggt tggagcgagc attgcctcgg ccgaaagccg ggcaaaatac ttcgcctttc
      901 aatccgccgt tcttgagagc gctttcgagg tgaagcgcgc gtattaccaa ctttactttt
      961 tggaggagaa gatccgcgtt aatcaggaaa cactgaggct gctggctgac ctggagaggc
     1021 tcgcccgggc tcaaaatgag gtgggcaaag tcacactgca agatgtgctg cgcgctcaaa
     1081 tcgagcagga ccggctcaaa accgacatcg ccaacctcga tgattcgcgc cgctcgctcc
     1141 tggcccagtt caaggctgcg ctgggtctgg gggccgacga tccaacaccg cccatccctg
     1201 gccggttcga atccaccccg ctggacctca ccgctgataa actgttcgag acggccctcg
     1261 cacagaatac gcgcctcaaa gctatggaag cggacgtgcg cgccgcggaa gccactatca
     1321 tcctcgcccg aaaggcgcgg ctgcctgatt ccaccctggg gctgatggcc gacgtgaaga
     1381 tggaccctac tctttatcgc ccgctgggca ccgtctcgct ccccatctgg cgcgacaaaa
     1441 tcgcggcaca aatcgccgag gctcaggcca accagcgctc ggctgaggcg cgcttaacgg
     1501 gcgagcagat cgcgctggcg gtggattttg ccgagaggtc gttcctctac cgtgaggcga
     1561 cccgcaatct ggcactcctg aatgataaac tcttgcccaa ggcgcgccgg tcgctcgaag
     1621 tcgcccggtc cggttacctc gccggccaga ttgacttctt caacctgacc gacaccgaac
     1681 gcaccctgct cgggttcaaa ttggataagg tggaagccac cacacaacgc gaagtcgtcc
     1741 tggcggagtt gtccttgatc attcagggca tgccggcggc caatgcttcc atggcttcga
     1801 agacttccgg catgcctcgc ggcaaaccct ctgcgtcgaa gaaaatgggt ggaggcatgt
     1861 gatctccggc aaaccaatta ttctcttatg aaattccatg ccttaagcgc gaaatccact
     1921 gttctgttca tgctggcctt cctgattgcc gctggcgccg ggctgttact tagcggctgt
     1981 ggtcgcgctc aggccccggc cgccaacgcc gacgcggcca aaacccttta cacctgcggc
     2041 atgcatccgc aggttgtcca acccacgccg ggcaactgcc cgatctgcgg catgaaactc
     2101 acccccatcc gcaagcaaat ggaagccgct aagggcagct cggccacgcc atccggcgag
     2161 cgcaagatca aatactacaa gtccaccatg acaccgggcg aagtgcggcc gacgccgggc
     2221 aaggatacaa tgggcatgga aatggtcccg gtctatgaag atgaggccac cgccgccgct
     2281 tcgcagacca tcgcgattga cccggtgaca acccagaaca tgggcatccg caccgcagtc
     2341 gttacccgcg ggccgcttcg tcgcaccatt cgcacggtag gcgccattga ctataacgaa
     2401 accgccctgg aggacgtgac caccaagttt aaaggctgga tcgagaagct ttacgtggat
     2461 gccaccggcc agcaggtgca tcgcggagat ccattgttcg agatttactc gcccgagctt
     2521 tacagcgctc agcgcgaata cttgcttgcg atcgaggacg gaacgaatgc cgccgacggc
     2581 gcttcgctca agaccagcgc gctcaccaag ctgaggtttc tcgacatctc ggccggccaa
     2641 atcgccgaaa tggagcgcac cgggcagccc actaaaacct tgcgcatcct ggcgcccatg
     2701 gacggtgttg tggtcgaaaa gatggtggtc cagggccaga tggtggaccc gggcatgaag
     2761 ctctaccggc tggcggatct gggtttggtg tgggtgcaag cgcagattta tgagcaggac
     2821 ctcgcctacc tgaagctggg tcaggaagcc accgtcaccc tctcctacct ccccgaccgc
     2881 gagttccgcg ggcgagtgac ttacatctat cccaacgtgg acgagaagac ccgcacggca
     2941 cgcgtgcgca tggaattcca caaccccggc tatttcctca agcccggcat gttcgccacc
     3001 gtccaggtct cctccgagct ggaagcttct gtgctgctgg ctcccgatat ggccatcttg
     3061 cgcagcggcg agaagaacac cgtcttcgtc gccttggagg aaggcaagtt cgaaccgcgc
     3121 accattgttt tgggaccaca agccgacaac gacatgtacc aaatcatcag cggactgaag
     3181 gaaggcgaac ggatcgtcac ttccggccag ttcatgctcg attcagaaag ccagctccgc
     3241 gaggccattc agaagatgct agagcccaaa caagccagcc agccccagtc tgctgttacc
     3301 ccccaaaacc cccctgatac ggctgcccac gcaggccatg aaagtatgac gatgcctgcc
     3361 gggaccctat ccgaagaggc aaaagcagtg aaatacgtct gcccgatgcc ggagcacatc
     3421 tccatccagt atgatcatcc gggcaagtgc cccatctgcg ccatgacgct cgtgccagtg
     3481 tccggcgcca cgctgaagaa actgcagccg ggcgggaagc tgctttatta cacatgcccc
     3541 atgcccgagc acagcgacgt gcgctccgac aaaccgggca aatgccccaa gtgcgccatg
     3601 acgcttattc ctgtgctgca ggcgccgccc atctcaaata ccgctacgaa tcaggccagt
     3661 gctgacaaaa ctcaggcctt gcccaccacc ctttacacct gcccgatggc gtcccacgcg
     3721 gatgtggtgt cggacaaacc gggcaagtgc cccaagtgcg aaatggaact ggtgccgacc
     3781 agcaccgtgt cccatggcaa gatcgcggag gagaactggc gcaagcagca ttcaggtgct
     3841 ccgccaagtg atgtcacgat gcctccacaa cgccaacact gagggcgcta ctcatgctca
     3901 aagacatcat agatttttcg ctcaagaaca agttcatcgt cctgctgggg acgattgcct
     3961 tggtgttggg cggactttac gcgctcaaga acatcccgct ggacgccatc cctgacctgt
     4021 cagacgtcca ggtcatcgtc tataccgagt ggccaggcca ggcgccgcag atcgtccaag
     4081 accaggtcac ctatccgatc accaccaaga tgctctcggt gcccaaggcg aaggttgtgc
     4141 gcggctactc gttctacggc ttctccttcg tgtatgtcat cttcgaggat ggcaccgatc
     4201 cctactgggc gcgcacccgc gtgctcgaat atctcagcgg catgagcggc cgcctgcccg
     4261 ctggcgtttc accaagcctc gggccggacg cgaccggggt gggctgggcg ttcatgtatt
     4321 ccatcacctc caccaatcga gatctggcgg agttgcgctc catgcaggac tggtatctgc
     4381 gctaccaatt ggcttcggtg gaaggcgtat ccgaagtcgc ctccgtcggc ggcttcgtga
     4441 agcaatacca gatcaccgtt gacccgacca agctgcgcgc ctacaacctt tccatctccg
     4501 aagtcgccat ggcggtcaaa cgcagcaatg gcgaggtggg tggacggtcc attgagctct
     4561 cggaaaaaga gttcatgctg caagtgcgcg gctacatcca gagtcttgag gatttgcgca
     4621 aaattgctgt gggtgtcgga gacaaggggg tgcccatcct cctccgacag gttgccaacg
     4681 tggaatttgg cccggatatg cggcgcggca tcgccgagct caatggcgaa ggggagaccg
     4741 tcgggggcgt ggtggtcgtc cgctacggcg ccaacgcccg gcaggtcatt ctcgatacga
     4801 agaagaggct ggatcaggcc atgaagggcc tcccggcgga cgtccagctc accgttgtct
     4861 atgatcgcac cgccctcatc aaccgtgcgg tcaaaacact cgaaggcaag ctcatcgagg
     4921 aaagcatcgt tgtggcactc gtctgtctgg ccttcctcat gcacctgcgc agcgcgttcg
     4981 tcgcgatctt gattttgcct atcgcggtgc tgatgtcttt tctcgtcatg ttcggccagg
     5041 gcatcagttc caatatcatg tccctaggcg gcatagcgat cgcgatcggc gccatggtgg
     5101 atgccgtcat catcatgatc gagaacgccc acaaacacat ggagcatgac cagggcaaga
     5161 agcctcactg ggacatcatc cgcgacgccg ccatcgaggt aggcccgacc ctgttctatt
     5221 cgctcctggt cattacggtt tccttcctgc cggtcttcac gcttcaggcc caggaaggcc
     5281 gcatgttcaa accgctggcg tttaccaaaa cctactccat ggcggcggct gcgattcttt
     5341 ccattaccct ggccccggtc ctcatgggcc tcttcatccg tgggaaaatt ccagcggagg
     5401 agaaaaaccc cctcaaccgc ttgctcgtct ggctctacca tccgatgatt gatttcgtca
     5461 ttaaatggcg ctggccggtc atctttactg ccgccggcct gatcgtttgg gtgttcttcc
     5521 cttggaacag tctggccgcg cgcctcctgc ccgatggcaa ggcaaaggaa tgggccttta
     5581 aggtcgggaa gtttttcccc tatcagagcc tcggctcgga gttcatgccg ccgctctatg
     5641 aaggagacct gctctacatg cccaccacct ttccaggcat ctcacccacg aaggcgcgcg
     5701 aaatcctcca gaccaccgac aagatcatcc gccgctttcc ggaggtgcac cacgtcttcg
     5761 gcaaagtcgg ccgcgccgag agcgccaccg atcccgctcc cttcgacatg atcgaaacca
     5821 ccatcatgct caagcccgaa gccgaatggc cggtggtcga tatcaaagac gacgcgggca
     5881 agatcatcgc ccaccggcgc cgcaccccgg acgagctcac cgacgtgatg aacgctgccg
     5941 tgcaaatccc gggcctgaac aacgcctgga ccatgcccat caagactcgc accgatatgc
     6001 tggccaccgg catcaagacc cccgtcggca tcaaggtcgc cgggcccgac ctcgccgaac
     6061 tggagcgcgt ggcctcggag attgaggccg tggtgaaggg tgtccccggc accctcagcg
     6121 cctttgccga gcgcaccatg ggcggcaatt acatccagtt caatattgac cgcgatgcca
     6181 tcgcccgcta cggcttgacc attggggatg tccaggacgt gctcgaagtc gccttgggcg
     6241 gcatgccctt gacc
//
LOCUS       DATCGS010000520         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_143725, whole genome shotgun sequence.
ACCESSION   DATCGS010000520 DATCGS010000000
VERSION     DATCGS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143725"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VIL39_10660"
     CDS             complement(<1..1383)
                     /locus_tag="VIL39_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY5481933.1"
                     /translation="MKPNSTPAPNHASPQVTQVTHLTPPRWAFCLSLLLALTLPALAN
                     LSPDRLRCEYLDNPLGIDTPQPRLSWVLESKQRAQKQTAYQILAASSETLLKSDSGDL
                     WDTGKVASDQTLHVVYAGKALGSCQRCYWKVRVWDKDGKPSAYSRPASWEMGLLSPQD
                     WQGQWIGRTTDTNSLPAPMLRRAFTLEGKIKRARAYICGLGYYELHLNGKQIGDHLLD
                     PGYTRFDRRALYVTYDVTDALRHGKNAVGVILGNGWYNVQTRAVWDFHKAPWRAAPKL
                     LMQLRVEYADGRIETIGTDSRWTTSAGPITFDSIYGGETYDARAEKPGWDTPDYDDSA
                     WATARVVAAPGGKLAAQMMPPIKADRIIKPVKLTEPQPGVFVFDMGQNFAGFAKLSVR
                     GPAGTKVVMKYGERLGKDGMLDRKDIEQHIVRVDRNQQYQTDTYILDGASRITHHASR
                     FTYHGFQYVEV"
     regulatory      1750..1872
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2125..3126
                     /locus_tag="VIL39_10665"
     CDS             2125..3126
                     /locus_tag="VIL39_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481934.1"
                     /translation="MLKTRLSVLALAGVSLLCVSHPLSLAAEDIFLTEDFAAPPDQRG
                     WQAFGATNLFGWNATNQNLEVTWDSSQPNSAFYHPLGAVLSKTNDFILEFDLRLADVA
                     IGTTSDKPYTFELALGLTDLASVTNMGFLRGTGFNSPNLVEFDYFPDSGYGATISTAV
                     ISSNNEWNDGGFTYPLALTTGDVFHVEMRFVAANQTLMTRMTRNGEPFGPIQQATLGA
                     NFSDFHVDQMAISSYSDAGQDPGYAGSILAHGTVDNFVFICPPPVSDLTGSFAGQTWQ
                     VQFTSHTNWLYTLERTGDFQTWVAAASTVAGTGGRLTLSDTNAPAGNAFYHVRAVRP"
     gene            3123..>3519
                     /locus_tag="VIL39_10670"
     CDS             3123..>3519
                     /locus_tag="VIL39_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5481935.1"
                     /translation="MNGTVESQESRAEGRGPAGVSRLTVNVPDFTSRAFTLVELLVVI
                     AIIAILAALLLPALARSRTSAQRIKCASNLRQLGLATQLYWDDNAGDCFRYLFGPTNV
                     GQIYWFGWLGDGAEGQRPFDVTQGALYPYL"
BASE COUNT          633 a         1064 c         1109 g          713 t
ORIGIN      
        1 gacctcgaca tactggaagc cgtgatacgt gaaacgtgat gcgtgatgcg tgatgcggga
       61 ggcgccgtcg aggatgtagg tgtcggtctg gtactgctgg ttcctgtcca ccctaacgat
      121 gtgctgttcg atatccttgc ggtcaagcat gccgtctttg cccaaacgct cgccgtactt
      181 catcaccacc ttcgtgcccg cgggtccgcg cacgctcaac ttggcgaagc cggcgaagtt
      241 ctggcccatg tcgaacacga agacgccggg ctgcggttcg gtgagtttga ccggcttgat
      301 gatccggtcg gccttgatgg gcggcatcat ttgcgcggcc agcttgccgc cgggggctgc
      361 aaccacccgc gccgtagccc atgcggaatc atcgtagtcc ggcgtgtccc agccgggctt
      421 ctccgcgcgg gcgtcgtagg tttcgccgcc atagatgcta tcgaaggtga tcggcccggc
      481 actggtcgtc cagcggctgt cggtgccaat agtttcgatg cggccatcgg cgtattccac
      541 gcgcaactgc atcagcagtt tgggcgcggc gcgccatggg gctttgtgaa agtcccacac
      601 tgcccgggtc tggacgttat accagccgtt gccgaggatc acgccgacgg cgttcttccc
      661 atggcgcagg gcatcggtga catcgtaggt gacgtagagc gcgcgtcggt caaagcgggt
      721 gtaacccggg tcgagcaggt ggtcgccgat ctgcttgccg ttgaggtgaa gctcataata
      781 gccgaggccg cagatgtagg cccgggctcg cttgatcttg ccctccagcg tgaaagcgcg
      841 gcggagcatg ggcgccggca gggagttggt gtccgtggtg cggccaatcc actggccttg
      901 ccaatcctgc ggggagagca gccccatttc ccaggaagcc ggccggctgt aggcggaagg
      961 ctttccgtcc ttatcccata cgcgcacttt ccaatagcaa cgctggcagg agccgagcgc
     1021 cttgccggca tacacgacgt gcagcgtctg gtccgatgcc accttcccgg tgtcccacag
     1081 gtcgccgcta tccgacttca acagggtctc gctactggcc gcgagaatct gatatgcggt
     1141 ttgcttttgc gcccgctgct ttgattcgag cacccagctc aaccgcggct gcggggtatc
     1201 aatgcccagc gggttgtcca ggtactcgca gcggagccgg tcgggggaga gattggccag
     1261 cgcggggagg gtcagggcga ggagcaggga aaggcagaag gcccatcgag gaggagttaa
     1321 atgagttacc tgggttacct ggggggaggc atggttgggg gcaggagtgg agttgggctt
     1381 catcgaattc aggcggttgg ctattcttgc gcacttgcta atatcggtca tcgcgtcgca
     1441 ttcatggcct gccacaagcg gacaatcaag actatttcag aacgcgggcc tggcctggcc
     1501 gcctcaacac tgccccggag cgcggacatt cccatccgcc gcaacgttcg atggataaga
     1561 cggcttggga actggccgga acgcgttacc tttcggagct tgctgcggac aggaatgtcc
     1621 gcgctccggt ggtggtgccg agacgcgccc ggaagattct tcttggcaag cagggccgaa
     1681 aggactagga atgaggcgct ctttgaaacc aatggactcg tcgtccatca ttaacactgc
     1741 caaacgtcag ggaaccccgt gagaatcggg gacggttgcg ccactgtaac gggctacaag
     1801 ctcccgaagc cactggtctc ctcagagacc gggaaggcgg gagtaaggtt ctaagcccga
     1861 agtcaggata tcggcttggc ggtgctcgtc gcggctctca gtgcgaaaca ggcagaatgc
     1921 ctgtcctgca ttgggggcta acttctccgt caaagagaag gatgaggcca gcccgtcgcg
     1981 caagtgcgtg ccgggattcg ctgattgcct tcattctccg atttgccggg gtctggaggt
     2041 tttttgttat cttctggccc cgcgctggtc tctcaatcgt taacccgctc cgcgctagtc
     2101 gcggagcatt tgaaagatca gcctatgctt aaaacccgtt tgtccgtgct ggccctggcc
     2161 ggcgtctccc tcctttgcgt ttcgcacccc ctgtcgcttg cggcggagga catcttcctc
     2221 accgaggact tcgccgcccc ccccgaccag cgagggtggc aggccttcgg agccacgaat
     2281 ctgtttggct ggaatgccac caaccagaac ctggaggtca cttgggactc ctcccaaccg
     2341 aacagcgcat tttatcaccc gctgggcgcg gtattgagca agaccaacga cttcatcctg
     2401 gagttcgacc tgcgtctggc cgacgtggcc attggcacga cgtccgacaa gccctacacc
     2461 ttcgagctcg cgctggggct gacggacctg gcgagcgtca cgaacatggg ctttctgcgc
     2521 ggcactgggt ttaattcgcc caacctcgtg gaattcgatt acttccctga ttccgggtac
     2581 ggggcaacca tttccactgc ggtcatttcc agcaacaatg agtggaacga cggcggcttc
     2641 acctacccgc tggcgctgac cacgggggac gtgtttcacg tcgagatgcg tttcgtcgcc
     2701 gccaaccaga ccttgatgac gcggatgact cgcaacgggg agccgttcgg ccccatccag
     2761 caggcgaccc tgggtgccaa ctttagtgac ttccacgtgg accagatggc gattagcagc
     2821 tatagcgatg ctggccagga tcccggctat gccggctcca ttcttgcaca cggcaccgtg
     2881 gacaattttg ttttcatttg tccgcccccg gtgagcgacc tcaccggcag cttcgcgggc
     2941 cagacctggc aggtccagtt caccagtcac acgaactggc tctacacgct ggagcggacg
     3001 ggcgacttcc aaacgtgggt ggcggctgcg agcacggtgg cgggcaccgg cggcaggttg
     3061 accttgtcag acaccaacgc gccagccggc aatgcctttt accacgtgcg agccgtccgg
     3121 ccatgaacgg aacagtcgag agccaagagt cgagagccga gggccgcggg cctgcgggcg
     3181 tttcacggtt aacggttaac gttccggact tcacgtcccg cgccttcacg ttggtcgaac
     3241 tgctggtggt gatcgcgatc atcgccattc tggcagcgtt actgctgccg gcgctggcac
     3301 gaagccggac ctccgcgcag cggattaaat gtgccagcaa tctgcgccaa ctgggactgg
     3361 cgacccagct ttattgggac gacaacgccg gcgactgctt tcgctacctg tttggcccaa
     3421 ccaacgtggg acagatctat tggttcggct ggctgggcga cggcgcggaa ggccagcggc
     3481 cattcgacgt cacgcaaggc gcactgtatc cctatctgc
//
LOCUS       DATCGS010000521         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_194075, whole genome shotgun sequence.
ACCESSION   DATCGS010000521 DATCGS010000000
VERSION     DATCGS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194075"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..986
                     /locus_tag="VIL39_10675"
     CDS             150..986
                     /locus_tag="VIL39_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5481936.1"
                     /translation="MFYHHLRNELWKLFGKKRTYIGFGAFLVAQNGMLLTFRFTHWQA
                     QMENMLAGNGYLAQEYVSALTVAVLMLIPQILLLMPLYAALVGGDLVAKEVEDGTLRM
                     ILSRPISRFRLLFVKWMAGGIFSAVLVLVLGGTALGFARMWFPWKGMFIFVPGQVFNV
                     LSAGDGFKLYLLSHLFMTLNASVVLGIAFMFSCFNMKPAAATILALSLLFVNLVMEGI
                     PFFEQYHEYLLTYHFRSWHNVYAQPIPWAQIGQSVCVLLAFNLTTFLIGAAAFQVRDI
                     KS"
     gene            1213..1866
                     /locus_tag="VIL39_10680"
     CDS             1213..1866
                     /locus_tag="VIL39_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5481937.1"
                     /translation="MRELIEAKRAWSRPLAQDEIQRGSLGWHESGYLPHRDEPGLVQF
                     VTFRLVDAFPAELRSEWEGLLQIEDDRKRRIELEAYLDRGRGTCHLRRPEIAKLVEGA
                     LLFRHGVEYDLRAWAVMPNHVHLLFLVQDVPMWRLVDAWKGYTAKEANKVLGRKGPFW
                     QEGYWDTYMRDCEHEARTRQYIENNPTKAKLVVLRKDWPWSSARFRDAYERLCLPAA"
     gene            2232..>4669
                     /gene="gyrB"
                     /locus_tag="VIL39_10685"
     CDS             2232..>4669
                     /gene="gyrB"
                     /locus_tag="VIL39_10685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY5481938.1"
                     /translation="MPDENAVEATEKAPVTTAAPEKYDASKIDKLEGLEAVRKRPGMY
                     IGDPDERGLHHSVFEVLDNSIDEHLAGYCSKIEVTVHVDGSVSIRDNGRGIPVDMHPK
                     WGMPAVELVLTNLHAGGKFGQGAYKYSGGLHGVGAKCVNALSDWFKVEVSRDGKVYSM
                     EFARGVTTQKLTVVGKSKGTGTLITFKPDPTIFTLTTEFKFDILANRLRELAFLNPGL
                     IIVLTDERDEKTETFLYKDGIEEFVKQLGRTKQVLHPKPIVINRQKDEVFVDCVMQYT
                     DSYNDQILCFANSISNPDGGTHLTGFRSALTRAINQYAKANEILKEKDPAISGDDVRE
                     GLVCVLSVKLPNPRFESQTKVKLVNTEIDGLVSSVIYDGLMTFFDANPAVAKKVIDKA
                     LLAARAREAARKARETVRKGALTGGGLPGKLADCSDRDPINTELYIVEGDSAGGSAKQ
                     GRDRKFQAILPIRGKIINVEKARLDKVLQNTEIRTMITAVGTGIGDGEGEGAFNLEGL
                     RYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELVRRGFIYIAQPPLYQIARKKRVEYV
                     DDDTRLNQILIELGTNEVRLRNLSDGNELTDKQLAEILELLESLDKYAIALRRHGGDF
                     TTYVEQRHPTTHELPRHLVKVRDGNDETVQYFHSEEDLEEFGSKNPDLHLFGDEESDT
                     SLLEKGKNGHTRRARHVELHESKAVAELLAKLAKKGLSVEHYSAQDKPLFEVIEGEGD
                     KEQVKPLFSIPEILASVKEVGRRGLSIKRFKGLGEMNPKELFETTMNPVRRKLLRVDL
                     TDAIEAEEMFAKLMG"
BASE COUNT          958 a         1304 c         1499 g          908 t
ORIGIN      
        1 aaggaaagtg aatgagttgc ctggttacat cgttacatgg ttacaaggtt acaaggttac
       61 atgggttaca agagttacaa tgactgcccc gcacgtgacg ccttatgtaa ctcatgcaac
      121 cttgcaaccc atgtgacttc ccaatgagta tgttctacca tcacctgcgc aacgagctgt
      181 ggaagctctt cgggaagaag cgcacctaca tcggcttcgg cgcgttcctg gtggcgcaga
      241 atgggatgct cctcaccttc cgcttcaccc actggcaggc gcagatggag aacatgctgg
      301 caggcaacgg ttacctggcg caggaatatg tctcggcgct tactgtggcg gttctgatgc
      361 tgatcccgca gatcttgctg ctgatgcctt tgtacgcggc gctggtgggg ggtgacctgg
      421 tggccaagga agtcgaggat gggacgctgc gtatgattct gtcgcggccg atttcccggt
      481 tccggctgct gtttgtgaag tggatggccg gggggatctt ctcggccgtg ctggtgctgg
      541 tgctgggcgg gacggcgctg ggttttgccc ggatgtggtt tccgtggaag gggatgttta
      601 tcttcgtgcc ggggcaggtg ttcaacgtgt tgagcgcggg ggacggtttc aagctgtatc
      661 ttttgtccca cctgtttatg acgctcaacg cgagcgtggt gctgggcatc gcgttcatgt
      721 tctcgtgctt caacatgaag cccgcggcgg ccacgatcct ggcgctgtcg ttgttgttcg
      781 tgaacctggt gatggagggc atcccgtttt tcgagcagta tcacgagtac ttgctgacct
      841 accacttccg ctcctggcac aacgtctatg cccagcccat accctgggcg caaatagggc
      901 aatcggtgtg cgttttgctg gcctttaacc ttaccacctt cctcattggg gcagccgcat
      961 ttcaggtccg cgacatcaag tcatagagaa cgggtccggg gaagcggggc cggccctggc
     1021 ggagtgcgcc cgtccccggg cgcagcagcg tagtgcgtag ctgggcgtgc ggacaactgg
     1081 cacgcgcttg aacatacgga cgttgctgcg gccgggacgg ccgcactccg ggctggtcat
     1141 cggtcgcgcc ttgcgctttc ttaacccagg atgaaggcta aacgattgcc cagcccgcct
     1201 aataatccgg gcgtgcgcga acttattgaa gccaaacgtg cgtggtcacg tccgcttgcc
     1261 caggacgaga ttcagcgtgg gtctctgggc tggcacgaga gcggttatct gccccaccgc
     1321 gacgagcctg gccttgtcca gtttgtcaca ttccgtctgg tggatgcgtt cccggcggag
     1381 ctgcgttcgg agtgggaagg gttgctccag atcgaggatg accgcaaacg gcgcatcgag
     1441 ctggaggcgt atctcgatcg agggcgaggg acgtgtcatc tacgccgccc ggagattgcc
     1501 aagctggtgg aaggagcgtt gttgttccgt cacggcgtgg aatacgacct ccgcgcctgg
     1561 gcggtaatgc cgaatcatgt ccacctgctt ttcctcgtgc aagacgtgcc aatgtggcgg
     1621 ttagtggatg cgtggaaggg ttacacggcc aaggaggcga acaaagtcct tgggcgcaag
     1681 ggaccgttct ggcaagaggg atattgggat acctacatgc gcgactgcga gcatgaggcg
     1741 cgcacgcggc agtacatcga gaacaacccg acgaaggcaa agctcgtggt gttgcggaaa
     1801 gattggccct ggagcagcgc gcggttccgc gatgcatacg agcgcctgtg cctgccggca
     1861 gcctgacgga gtgcgcccgt ccccgggcgc agcaatgtaa gacctgatga gacgcgtgga
     1921 agattcgtag gcgccctgag ttgcggacgt tgctgcgccc gggaacgggc gcactccgcc
     1981 aaagttggct tccggcccca ttggctcatt attacggcag gttgcatttg gtggcaggga
     2041 atcgcaaatt aagccagtca ggcaggcatt ctccaatatc ccaatatata ggttgcggcg
     2101 gcagaccgcc ccacaactaa tggtgttttt gtggcaaaaa ggcccgaaat aggagttgtg
     2161 ttccctggcc aattttccta ctatttttgg tcctgaaacg gacggccaaa gtgcctggtc
     2221 caaatgaatt tatgcctgac gaaaatgcgg ttgaagcgac agaaaaagcg ccggtaacca
     2281 ccgccgcgcc cgagaagtat gatgcgtcga agattgacaa gctcgagggg ctggaagcgg
     2341 tgcgcaaacg cccggggatg tatatcggcg acccggacga gcgcggtctg caccacagtg
     2401 ttttcgaggt gctggataat tccattgatg agcacctggc tggctactgc agcaagatag
     2461 aagtcacggt ccatgtggat ggctcggtgt ccatccgtga caatgggcgc ggcattcccg
     2521 tggacatgca tccgaaatgg ggcatgccgg cggtggaact ggtgctgacc aacctgcacg
     2581 cgggcggcaa gttcgggcag ggcgcctaca aatactccgg cggcttgcac ggcgtcggcg
     2641 ccaagtgtgt caatgcgctg tcggattggt tcaaggtgga agtgtcgcgc gacgggaagg
     2701 tctattccat ggagttcgcg cgcggcgtca ccacgcaaaa gctgaccgtc gtcggcaagt
     2761 ccaagggcac cggcacgctg atcaccttca agcccgaccc gaccatcttt accctcacca
     2821 ccgagttcaa gttcgacatc ctggcgaacc gcctgcgcga gctcgcgttc ctgaacccgg
     2881 gcctcatcat cgtcctgacg gatgagcggg acgagaagac cgagaccttc ctgtacaagg
     2941 acggcatcga ggagtttgtc aagcagctcg ggcggaccaa gcaggtgctc catcccaagc
     3001 cgatcgtgat caaccggcag aaggacgagg tgttcgtgga ttgcgtgatg caatacaccg
     3061 acagctacaa cgaccagatc ctctgcttcg ccaactcgat ttcaaatccg gatggcggca
     3121 cgcacctgac gggcttccgt tcggccctga cccgcgccat caaccaatac gccaaggcga
     3181 acgagatcct gaaggaaaag gacccggcca tctcgggcga tgacgtccgc gaggggctgg
     3241 tgtgcgtgct gagcgtgaag ttgcccaacc cgcggtttga gtcgcagacc aaggtcaaac
     3301 tcgtcaacac ggagatcgac ggcctcgtgt cgtcagtcat ctacgacggt ttgatgacgt
     3361 tctttgatgc aaacccggcg gtggccaaga aggtgattga taaggctttg ctggcggcgc
     3421 gggcgcgcga ggcggcccgc aaggcgcgcg agacggtgcg caagggcgcg ctgaccggcg
     3481 gcgggctgcc cgggaagctg gccgattgct cggaccggga cccgatcaac accgagctgt
     3541 atatcgtgga aggtgactca gccggcgggt cggccaagca agggcgcgac cggaagttcc
     3601 aggccatcct gcccattcgc ggcaagatca tcaacgtgga gaaggcgcgc ctggacaagg
     3661 tgctgcagaa caccgaaatc cgcaccatga tcaccgcggt gggtacgggc attggtgacg
     3721 gggaagggga gggcgctttc aacctggaag gcctgcgcta ccacaagatc atcatcatga
     3781 ccgatgcgga cgtggacggt gcgcacattc gcacgctgct gctgacgttc ttctatcggc
     3841 agatgcctga actggtccgg cgcggtttca tctatatcgc ccagccgccg ttgtatcaga
     3901 tcgcgcgcaa gaagcgggtg gagtacgtgg atgacgacac ccggctgaac cagatcctca
     3961 tcgagctggg caccaacgag gtccgcctgc gcaatctgtc cgacggcaat gagctgaccg
     4021 ataagcagct tgccgaaatc ctggaactgc tcgaatcgct cgacaagtat gccatcgcgt
     4081 tgcgccgtca cggcggcgac ttcaccacct acgtcgagca gcgtcacccg acgacccacg
     4141 aactgccgcg ccacctggtg aaggtgcgcg atgggaacga cgaaacggtg caatacttcc
     4201 atagcgagga ggatttggaa gagtttggtt ccaagaaccc cgatctgcac ctcttcggcg
     4261 atgaggaatc cgataccagc ctgctggaga aggggaagaa cggccacacc cgccgcgccc
     4321 gccacgtcga gctgcatgag agcaaggccg ttgcggaact gctcgcgaag ctggcgaaga
     4381 agggattgag cgtcgagcac tactcggcgc aagacaagcc gctgttcgag gtcatcgaag
     4441 gcgagggcga caaggagcaa gtgaagccat tgttctcgat ccctgagatc cttgccagcg
     4501 tcaaggaagt gggccggcgc gggctctcga tcaagcggtt caaaggcttg ggcgaaatga
     4561 accccaagga actcttcgag accaccatga acccggtgcg gcgcaagctg ctgcgcgtgg
     4621 acctgacgga tgcaattgaa gccgaagaga tgttcgctaa actgatggg
//
LOCUS       DATCGS010000522         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_737337, whole genome shotgun sequence.
ACCESSION   DATCGS010000522 DATCGS010000000
VERSION     DATCGS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737337"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..903)
                     /gene="allE"
                     /locus_tag="VIL39_10690"
     CDS             complement(160..903)
                     /gene="allE"
                     /locus_tag="VIL39_10690"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEY5481939.1"
                     /translation="MTELFGITRNVVKGRYALLTPGGFVPSYLPGWEKAVCYTLISPA
                     LGARFSQLLITLERDGKCAGNTGANQYFIYVLEGSASILLEARKHRLEAGSYAYLPSG
                     QDVQIASGAAATRLLVFQKEYQPLPGVVKPTGFAAHEREIKGQPFRGNEDLRLQALLP
                     AHASFDMAINILTCQPGAVLPTVESPVMERGLMILRGQCVWRLEADWHPAQAGDVIWA
                     APYCPQWFVAMGKKPASCIYYQDVNREPM"
     gene            1004..2386
                     /gene="hydA"
                     /locus_tag="VIL39_10695"
     CDS             1004..2386
                     /gene="hydA"
                     /locus_tag="VIL39_10695"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY5481940.1"
                     /translation="MSLLVKNGEIVTAGERYMADIFCADETITRIEPNIPTPPGAEVI
                     DASGKYVFPGFIDPHVHIYLPCMGTFAKDTHETASKAALVGGTTTLIEMLGPDRTQEP
                     MAGFELWLGKAQGHCACDFTFHMTVSRFDEAAEKQLIEIVKCGISSFKIYLAYKGAFG
                     LTDQELYRTLRLARKLGVITTAHCENADLIQELQNHLLAEGKTGPEWHHASRPPIVEA
                     EGVHHLLTFAELLDAHVYIVHVSCEEALRAATAGKGRGVKVWTETLIQYLLLDKTYAE
                     RPDFEGAKFVMSPPLRDKRNQAVLWNGLRDGVISTLATDHAPFDFATQKRMGEKDFTK
                     IPSGIPSLEDRLNAFYTYGVKRGRVDLHRFVDAASTQAAKLFGLFPRKGTIQPGSDAD
                     LVIYDPSYRGTLSAKTQTMNVDYNPFEGMAIEGRPHVVTVRGQIAVRDGRFVGQSGRG
                     RFLQREPSHF"
BASE COUNT          544 a          817 c          801 g          490 t
ORIGIN      
        1 tacgagatta cccgcgagat cgcctcgttg cgtgagttaa acgactggct gggatagcca
       61 gggttgacac ttgccagcct caaaagatgt ctcctctatg gtttgagcga gggagtggca
      121 atacctcata ttcgcagggc cgcgcacagt gcctggaaac tacatcggct cccggtttac
      181 gtcctgataa tagatgcaac tggcgggttt cttgcccatc gccacaaacc attgcggaca
      241 ataaggcgcc gcccagatca catcaccggc ttgcgccgga tgccaatcgg cttccaggcg
      301 ccaaacacac tggccgcgca gaatcatcag tccccgctcc atgacgggcg actcgaccgt
      361 gggcagcacg gcaccgggtt ggcacgtgag aatgttgatg gccatgtcga agctcgcatg
      421 ggcgggcaga agggcctgca accgcaggtc ctcgttgccg cggaacggct ggcctttgat
      481 ttcgcgctcg tgggcggcga agcccgtcgg ttttacgaca ccaggcagcg gctggtattc
      541 cttttgaaaa accaggaggc gtgtggccgc ggctccgctg gcgatttgaa catcctggcc
      601 ggacgggagg taagcgtagc tgccggcttc gaggcgatgc ttccttgctt caagcaggat
      661 gctggcggag ccttcgagca cgtaaatgaa atactggttc gcgcccgtgt tgccggcgca
      721 cttgccgtcc ctttccagcg tgatgagcaa ttgggaaaag cgggcgccca gcgcgggaga
      781 gatgagcgta taacacactg ctttctccca gcccggcaag tagctgggca caaagccgcc
      841 gggcgtaagc aacgcatagc gccccttaac aacattacgc gtgatgccga atagttctgt
      901 catgtgtcca gttgaatacg ccatggagcc gcccttgcca agcccgggat ttgccaggcg
      961 ttttgtgctg tcaaaaccgc aggcgccggt ttacaaagcg cgcatgtctc tactcgtcaa
     1021 gaacggcgaa atcgtcactg ccggcgaacg ctacatggcc gacatcttct gcgccgacga
     1081 gaccatcact cgcatcgagc ccaacatccc gacgccgcct ggcgcggagg tgattgacgc
     1141 gagcggcaaa tacgtctttc ccggcttcat tgatccgcac gtgcacattt acctgccgtg
     1201 catgggcaca tttgccaaag atacgcatga gacggccagc aaagcggcgc tggtaggcgg
     1261 cacaacgacc ttgatcgaga tgctggggcc ggaccgcacc caggagccga tggcgggatt
     1321 cgagttgtgg ctgggcaagg cccaggggca ttgcgcgtgc gacttcacct tccacatgac
     1381 cgtgtctcgc ttcgacgaag cggcggagaa gcaactgatc gagatcgtca aatgcggcat
     1441 cagcagtttc aagatctacc tggcttacaa gggcgcgttc ggcctcaccg accaggagct
     1501 ttaccgaact ctacggctgg cccggaagct cggggtcatc accacggcgc actgcgagaa
     1561 cgcggacctg atccaggagc tccagaatca tttgctggcg gaaggcaaga ccgggcccga
     1621 gtggcaccac gccagccggc cgccgatcgt cgaagccgaa ggcgtccatc acctgctgac
     1681 ctttgcggaa ctgcttgatg cacacgttta tatcgtacac gtaagctgcg aggaggcttt
     1741 gcgcgcggcg acggccggaa aaggtcgcgg ggtcaaggtt tggaccgaaa cgctcatcca
     1801 gtatctgctg ctggacaaga cttatgccga acgcccagac tttgaaggcg ccaagttcgt
     1861 gatgtcgcca cccctgcgcg acaagaggaa tcaggcggtc ctgtggaacg gcctgcgcga
     1921 cggcgtgatc agcacactag ccaccgacca cgcgccgttc gactttgcca cacagaagcg
     1981 gatgggcgaa aaggatttca cgaagatccc tagtggcatc ccctcgctcg aagaccggtt
     2041 gaacgccttc tacacctatg gcgtgaagcg cggccgagtg gacttgcacc ggttcgtgga
     2101 cgcggccagc acacaagccg ccaaactctt cggcctgttc ccgcgcaaag gcacaatcca
     2161 acctggcagc gacgccgacc tggttatcta cgaccccagc taccgcggta ccctctcggc
     2221 caagacgcag acgatgaatg tggattataa cccgttcgag ggtatggcca tcgagggccg
     2281 cccccacgtc gtcactgtgc gcgggcaaat cgcggtgcgc gacgggcgat ttgtcggcca
     2341 gtccggtcgt ggccgctttc tccagcgcga gccaagccac ttttaggtaa tgggtcagtg
     2401 acggcggaga cgacggtgtc ggagtgcgcc cgtcctcggg cgcagcaagc cccgaatgcg
     2461 ctgacgcgcc ggatttcatt atatctctgc cgctttccaa cattgctgcg cccgaggacg
     2521 ggcgcactcc gccacgacct cctcccgctg tcactgaccc attacggccg ggcgagttgg
     2581 tcatcgggca accctgcgtg aggatgccgc cggtgccgag tggccaagac acttagtccg
     2641 agcttaccag ct
//
LOCUS       DATCGS010000523        11291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_439346, whole genome shotgun sequence.
ACCESSION   DATCGS010000523 DATCGS010000000
VERSION     DATCGS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439346"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2751
                     /locus_tag="VIL39_10700"
     CDS             <1..2751
                     /locus_tag="VIL39_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEY5481941.1"
                     /translation="ANTSIVHSGSKSAAVTITGAWGAFSLHHSDINTSTYSSLTFWLN
                     GGASGSQHLQIYAELGSTAQPPVSLAPLVANTWQQVTLSLASLGVASQPNFARFSIQD
                     RTGGAQPTFYVDDITLTTNSVPPPSVWLTAPTDGLAYSAPASISLAASVTPYGHTISK
                     VQFYSGASLLFEDTSTPYSFTWTNVGIGSYSLFARTIYDAGASVDSPAVNVTVTGTSP
                     VTITVDAQRNRHVISPLIYGVAFAPDASTVADLNTPLHRSGGNSETRYNWQTNAHNHA
                     ADWYFQSIADSPSTPAAAADSFVSDSKNGGAEPALTIPMIGWMPKLGPSRGKLASYSI
                     AKYGPQTGSDSQWMPDAGNGISSTNGNQPITWNDPNDANFLTNSTFQQAFVQHLTNRW
                     GLSSNGGVRYYIMDNEHSIWHSTHQDVHPNGASRAEIRDKFFDYAGMVKRLDPNALVW
                     GPEEFGWSGYLYSGADLQYGGQHGWGYLPDRSTNGGMDYMPWLLSQFYQRATNTNQRL
                     LDYFTLHCYPQSGEALSTDVSTSMQLLRNRSTRQLWDTNYVDASWINSIVMLIPRMKG
                     WVASYYPGTKIGITEYNWGAEDHINGATTQADILGIFGREGLDLATRWTTPASSTPTY
                     KAMKIYRNYDGNKSGFGDTSISATGPNPDNVSTFVAVRSSDGALTVMVINKQLTSGAI
                     TAVNLANFLPAGTAQVWQLTSANTITRLSDLSLSGSNFTSTVPVQSITLFVLPAAILA
                     GPASNPNPASGATGVAINSSLSWAGGTNATLHRLYLGVSSNAVANATTNASEFKGDLA
                     GASYAPGLLASSGRFYWRVDEMAGAYVTTGPVWTFATTVSGTATFPLGGSLGSGDAFV
                     IRFPSQVGQTYRVERSASLSPAYWSPVADNVPGTGAAIQIPDSGASLQAQRFYRVIIL
                     SP"
     gene            3282..3956
                     /locus_tag="VIL39_10705"
     CDS             3282..3956
                     /locus_tag="VIL39_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HEY5481942.1"
                     /translation="MKPNRKLAETTTPDGAHMVLYEHDGAYCIRLNGQDLMHSSVTAS
                     ELRLGELAAETLSKQPDSLVLVGGLGLGFTLKSLLAKGGPSARVQVAELIPQIVDWNR
                     SFLAHLNGTLLDDPRVEVLLRDVWTVIAGAEPARYDALVLDIDNGPTAMVQKQNARLY
                     SLKGLQQIASVLKPNGRAFFWSARPDPAFALRLARGGFKVQVVPAPLYPGAKRYAVSI
                     YVAEKQ"
     gene            complement(3961..5541)
                     /locus_tag="VIL39_10710"
     CDS             complement(3961..5541)
                     /locus_tag="VIL39_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5481943.1"
                     /translation="MQQTSKQINGSAKAGQPTNGDDALKRVRAATAILEALAADESML
                     AGISVEERARLLRAAGQVYCPDVRIRRRIIKNRERQRRAERQQRHDAVLAQTGIRKLR
                     QQPVFTTPNVFPPAGFEPTPGPEETSPGQVEEEQHCYICKRHYSQIHAFYDQLCPECA
                     EVNFRKRGELADLRGRVALLTGGRVKIGYQAGIKLLRSGAELIVTTRFPRDSAERYAR
                     EPDFEQWGHRLEIFGLDLRHTPSVEAFCQHLLKTRARLDFIVNNACQTVRRPPDFYQH
                     MMARETGPLQELSTKAARLLGGYEGLRGYHLLPEGAAGLPGHQSPKLAGLKHAAELSQ
                     VPLLPEEALAQRDLFPEGCLDQDLQQLDLRERNSWRLTLAEVPSVELLEVQLVNAVAP
                     FVLNARLKPLLLRTPERDKHIVNVSAVEGQFYRKSKTTRHPHTNMAKAALNMMTRTSA
                     TDYHTAGIHMNSVDTGWVTDEDAKQLAERKTRDHCFHPPLDIVDGAARIVDPIIAGFN
                     TGQHVWGKFLKDFQPTDW"
     gene            complement(5717..6919)
                     /locus_tag="VIL39_10715"
     CDS             complement(5717..6919)
                     /locus_tag="VIL39_10715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5481944.1"
                     /translation="MNAQQTTVLSLSFGVPILSALALFLVTGQAGGADSFAGVSGAMQ
                     ECISKQEAAGVVTLIATRDKVLHLEATGLADIAAKAPMRTDAICWIASMSKPITATAV
                     LMLQEEGKLSVDDPVAKYLPELGDLKTADGKPAKLTLRHLLTHTSGMPEATPEQYKTA
                     RSLADVIPFYVGKPLKFEPGSKWQYCQSGINSLGRIVEIVSGQSFPAFLQKRLLDPLG
                     MKDTTFYLSSEQLPRLAKTYQRTEATLQEAPISFLNGHSPTDHDRYPAPNGGLFSTAP
                     DYAKFCRMILNQGTLDGKQYLKPASVKLMTSIQTGDLKTGFTPGNGWGLGWCVVQQPQ
                     GITAMLSAGTCGHGGAYGTQAWIDPVKQVIYVLMVQRSNFANADDSEVRRAFQAAAAS
                     SLGFRAER"
     gene            7214..7486
                     /locus_tag="VIL39_10720"
     CDS             7214..7486
                     /locus_tag="VIL39_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEY5481945.1"
                     /translation="MAQAIMDPEQVRRFAEELKRFNTDLKDKIVSLQARFAGLGDTWQ
                     DQEHIKFSEEFKQTMKALKKFVEISNQHTPYLLRKAQRIEEYLNQR"
     gene            7491..8030
                     /locus_tag="VIL39_10725"
     CDS             7491..8030
                     /locus_tag="VIL39_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481946.1"
                     /translation="MPERAHVTSVDALESFRSNLIVYLSNARPTLEGVSAAVQRTRGW
                     LEGEQRTYWESEFRRRSRAFQEAQAALFSSRMSRMREASAAEQMAVQRTKRALDEAEA
                     KLRVVKQWNRVFDNQVDPLVKQMEKLHTVLAHDMVKAVAFLAQAIQTLDAYAEVAPPT
                     AVPGSAQAGGQSAEPGNNP"
     gene            8027..8233
                     /locus_tag="VIL39_10730"
     CDS             8027..8233
                     /locus_tag="VIL39_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481947.1"
                     /translation="MKASSNRLSGITKELRAQWQDTKAYWKDAKSQEFEHRYMEELLA
                     GVDRTVTVIEQLDKLITKIRKDCE"
     gene            8226..>11291
                     /locus_tag="VIL39_10735"
     CDS             8226..>11291
                     /locus_tag="VIL39_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY5481948.1"
                     /translation="MSSNLGVNKTLELLDRLKGTVRKFGARADKLNQDFQGGTSRERR
                     HREALVEEQAKQLSAAISEAEGAFSAAKGAAGAKHEARKARIGKAYQASKETALGDIE
                     NRTGSRKYELQKRMLQAERDRAAGLTAAATTFEEFKANLTAEQAALTPLEMATHSAFK
                     GYRKLLRLLTDAYEKAALPAATLDENQLLAQLRELIGKTRGDLERFQRFWLLRVFKYL
                     AAWVVVVLCELPLVLHQTGPNSAGYWKAGLCVAGSLVVVLILRSLARSQAGPLAAAIA
                     GAIGKARRLHDTARERSEAHYQQELERIKGEFESTIQTVDQQLKQALAEAGERRVGCR
                     MQSDEKASRALEKNDRLWQAKQNQLQRQHAGDLEQLKRTAETRRKAEVEASEQREAKL
                     NTDYHAQWQTLEADWKQTIQPIYAAIESANATAGKLFPPWEPRVVETWAPPAQFAAAA
                     QFAKMAVDVEKLAETTIKDKRLALPGPARFSVPLCLGYPEQGSILFETAAAGHDEAIG
                     ALNNVILRLLSTAPPGRLNFTILDPVGLGQNFAGIMHLADYEEQIINSRIWTQSGQIE
                     QKLADLNEHMEKVIQMYLRNEYATIAEYNEQAGVIAEKYYFLVVADFPANFTETAAKR
                     LLSIAASGARCGVYMLIHWDQRQPLPPEFIPDELRDSSVCLSGKGNQFLLGGKPIPGT
                     DLVLDAPPPPEFAIEFVNKVGLASRDSSRVEVPFAHVAPSEAEIWTEDTSSELRVPVG
                     RTGATKFQYLAIGKGTCQHALLAGKTGSGKSTLFHVIITNLALWCSPEQVEFYLVDFK
                     KGVEFKCYATHRLPHARVVAIESDREFGLSVLQRVDDELRHRGDLFRQLGVQDVAGYK
                     RAGGKEPIPRSLLIIDEFQEFFVEDDRISQTASLLLDRIVRQGRAFGIHVLLGSQTLG
                     GAYTVARTTLGQMVIRIALQCNEADAYLIMDENNPAPRLLSRPGEGIYNDMAGMIEGN
                     SPFQVVWLPDQVRESYLAKVRARADAEEVQSLKSKVQSPGADGGQRYPGP"
BASE COUNT         2263 a         3457 c         3546 g         2025 t
ORIGIN      
        1 gccaatacga gcattgtcca ttctggctcc aagtccgccg cggtgacgat caccggggct
       61 tggggtgctt tctctcttca ccacagcgac ataaacacca gcacctattc gagcctcacc
      121 ttctggctga atggcggagc cagcggcagt cagcacttgc agatctatgc ggaattgggg
      181 tccactgccc aaccgccagt gagcctggcg ccgctggtgg cgaatacctg gcagcaagtg
      241 acgctttcgt tggcttcatt gggcgtggcc agccagccga acttcgcccg gttttcaatt
      301 caggacagaa cgggtggcgc acaaccgact ttctacgtgg acgacatcac tctgaccacg
      361 aactcggtgc ccccgccttc ggtctggctg accgccccga cggatggctt ggcctacagc
      421 gcgccggcaa gcatcagcct ggctgccagc gtcacgccct atggacacac cattagcaag
      481 gtgcagtttt acagcggcgc cagccttttg tttgaggaca cctctacgcc ctacagtttt
      541 acgtggacca acgtgggcat tggcagttac agcctgtttg cccggacgat ttacgacgcc
      601 ggcgcgagcg tggattcgcc ggctgtgaat gtgacggtca ccggaaccag cccggtcacg
      661 attacggtgg acgcgcaacg caaccggcat gtgatcagcc cgctgatcta cggcgtggcg
      721 ttcgctcccg acgcgagcac ggtggccgac ctgaacaccc cgttgcaccg ctcgggcggc
      781 aattccgaga cgcgctacaa ctggcaaacc aacgcgcaca atcacgccgc agactggtac
      841 ttccaaagca tcgccgactc accgtccacg cctgctgccg cggccgatag cttcgtcagc
      901 gacagcaaga acggcggcgc cgagccagcg ctgaccattc cgatgatcgg gtggatgcct
      961 aaactagggc caagccgcgg caagctggcc agttattcga tcgccaaata cggtccgcag
     1021 acgggcagcg actcgcaatg gatgcccgat gccgggaacg gcatcagcag caccaacggc
     1081 aaccagccca tcacttggaa cgatccgaac gacgccaatt tcctgaccaa ctccacgttc
     1141 cagcaggcct ttgtccaaca cctgaccaac cgctggggat tgtccagcaa tggcggtgtc
     1201 cgttactaca tcatggacaa cgagcacagc atctggcact cgacgcacca ggacgtccac
     1261 cccaatggcg ccagccgcgc ggaaatccgt gacaagttct tcgactatgc cgggatggtt
     1321 aaacgcctcg atcccaacgc gctggtgtgg gggccggagg aattcggctg gtcgggctac
     1381 ctctacagcg gcgccgacct ccagtacggc ggccagcacg gctggggcta tctcccggac
     1441 cgcagcacga acggcggcat ggattatatg ccttggttgc tgagccagtt ttaccagcgc
     1501 gccaccaaca ccaaccagcg cctgctggat tatttcaccc tccactgcta cccgcagagc
     1561 ggcgaggccc tctcgaccga tgtctccact tccatgcaac tgctccgcaa ccgctccacc
     1621 cgccaactct gggacaccaa ctatgtagat gccagttgga tcaacagtat cgtcatgctc
     1681 atcccacgca tgaaaggctg ggtcgccagc tattaccccg gcaccaaaat cggcatcacc
     1741 gaatataact ggggagcgga agatcacatc aacggcgcca ccacacaggc tgacatcctg
     1801 ggcatcttcg gccgtgaggg cctggacctg gcgacgcgct ggacgacccc tgcctccagc
     1861 acgccgactt acaaggcgat gaagatctat cgcaactacg acggcaacaa gtcgggcttc
     1921 ggcgacacca gcatctccgc cacggggccg aacccggaca atgtttcgac gtttgtcgcc
     1981 gttcgctcgt cggacggcgc gctgacggtc atggtgatca acaagcaact gacctcgggc
     2041 gccatcaccg cggtgaatct tgccaatttc ctgcccgccg gcaccgccca ggtctggcag
     2101 ctcacctcgg ccaacaccat tacgcggctg agcgacctca gtttgtccgg cagcaatttc
     2161 accagtacgg tcccggtgca gagcatcacg ttattcgtgt tgccggcggc catcctggct
     2221 ggaccggcga gcaatcccaa tccggccagc ggggcaacgg gcgtagccat caactcgagc
     2281 ctgagctggg cgggcggcac gaatgcgact ttgcatcgcc tttacctggg agtgagttcc
     2341 aatgcagtgg caaacgccac caccaatgcg tccgagttca agggcgacct cgcgggcgcc
     2401 agttatgctc cgggtctgct ggcctccagc ggccggtttt actggcgcgt ggatgagatg
     2461 gccggagcct acgtcacgac cgggccagtc tggacgtttg ccaccactgt cagcggcacg
     2521 gccacatttc cattgggcgg cagtctcgga agtggcgatg cgtttgtgat caggttcccc
     2581 agccaggttg gccagaccta ccgggttgag cgctcggcga gcttgagccc ggcatactgg
     2641 tcgccggtgg ccgacaatgt gcctggaacc ggcgccgcga tccagattcc tgactccggc
     2701 gcgtcgctcc aggcgcaacg cttctaccgg gtcatcattc tctctccctg accctcacgc
     2761 gatggagagg gtcggggtca gggggaaggg ctgttcaaca taagtagccc gaatgcaact
     2821 ctcattcagg agtcttcttg gcatactctg cgccgttagc agcgcggggc ccggctttgc
     2881 gggaaccctc acgcgcatta cctggacgtc acaccagggg agttatattc ggtatgctta
     2941 tgaaaccgat ttgtcgtccg gagatctgct cgttgcattc cagatgttgc ccgcaatggg
     3001 ggcacgaaaa cttgaaatga ctcataaagc ctgccagtaa tcgcgcgaaa gtgaccagaa
     3061 ggtttgcgtg agcgcattgt tggagcggaa atggaggttc ccagcggcgt catatccgta
     3121 ccccagttgc tcatgcaccc ggttggtggt gccgggactc cttgcctcgg gcgatttgca
     3181 gttggccgat cttgccaaac tgtgcgaaga atcgcataaa gcaggcggcg cctgccggtg
     3241 gaagaactgc cggaggagcc tgccgccatg gatagagttt tatgaaaccc aaccgaaagc
     3301 tggccgaaac gacaaccccg gacggagccc acatggttct ttacgagcat gacggggcct
     3361 attgtatccg gctgaatggt caggacctga tgcattcatc agtcacggcg tcggaactcc
     3421 gactcggaga actggcggct gaaacactct caaagcagcc ggattctctg gtgctggttg
     3481 gagggctggg tctcggcttt acgttgaaga gcctgctcgc caagggcggt cccagcgcga
     3541 gagtccaggt cgcggagctc atcccgcaaa tcgtcgattg gaatcgcagt ttcttggctc
     3601 acctgaacgg aacgttgctg gacgacccgc gggttgaagt gttgttgcgc gatgtgtgga
     3661 cagtcatcgc gggcgcggag cccgctcggt acgacgcctt ggtgctcgac atcgacaacg
     3721 gccccaccgc catggttcag aagcagaacg ctcgcctcta cagtttgaag ggcctccagc
     3781 agatcgccag tgtgctcaaa cccaacggac gcgcgttctt ctggtccgct cgcccagacc
     3841 cagcctttgc cctgcggttg gctcgcggcg gcttcaaggt ccaggtcgtc ccagccccgc
     3901 tttaccccgg cgccaaacgg tatgcggtga gcatctacgt ggccgagaaa cagtaaatcc
     3961 ctaccaatcg gtcggctgga agtccttcag gaatttgccc cacacgtgct ggccggtatt
     4021 gaagccggcg atgatcggat cgacgatccg cgccgcgccg tcaacaatgt ccaggggagg
     4081 gtgaaagcaa tggtcgcgag tcttgcgctc cgcgagctgc ttggcatctt catccgtcac
     4141 ccagcctgta tccacgctgt tcatgtggat gccggcggtg tggtaatccg tcgccgaggt
     4201 gcgcgtcatc atgttcagcg cggctttcgc catattggtg tggggatggc gcgtggtctt
     4261 ggacttgcga tagaactgcc cttccaccgc cgagacattt acgatgtgtt tgtcgcgttc
     4321 cggagtgcgc agcagcaggg gttttaaccg ggcattgagc acgaacggcg cgacggcatt
     4381 gaccaactgc acctcgagca gttcgaccga aggcacttcg gccagcgtca ggcgccagga
     4441 gttgcgctcg cgcagatcca gttgctgcaa gtcctggtca aggcatcctt cgggaaacaa
     4501 gtcgcgttgc gcgagggctt cttcgggcag cagcggcacc tgcgacagct ccgccgcatg
     4561 cttcaggcct gcgagcttcg ggctttgatg gccgggcagc cccgcggcgc cttcaggcag
     4621 caggtggtaa ccgcgcaaac cctcgtagcc tccgagcagt cgggcggcct ttgtggacag
     4681 ctcctggagc ggcccggtct cgcgcgccat catgtgctgg tagaaatccg gcggccgacg
     4741 caccgtttgg caggcgttat tgacgatgaa atcgagccgc gcgcgcgtct tgagcagatg
     4801 ctggcaaaag gcttccacac tgggggtgtg gcgcaaatcc aagccgaaga tttccagccg
     4861 atggccccat tgttcgaagt cgggttcccg ggcatagcgc tcggctgagt cgcgcgggaa
     4921 tcgcgtggtg acgattagct ccgccccgga gcgcaacagc ttgatgcccg cctggtaacc
     4981 gatcttcacc cgcccacccg taagcagggc cacccggccc cgcaaatcgg ccaattcgcc
     5041 tcgcttgcga aaattgacct ccgcgcactc gggacagagc tgatcgtaga aagcgtgaat
     5101 ctgactgtag tggcgcttgc aaatgtaaca atgctgctcc tcctctactt gtccggggga
     5161 ggtctcctcc gggcccggcg tgggctcaaa ccctgcgggc ggaaacacat tcggcgtggt
     5221 aaacaccggc tgctggcgca gcttccgaat gccggtctgg gccagcacgg cgtcgtggcg
     5281 ttgttgtcgc tcggcccggc gctggcgttc ccggttcttg atgattcgtc tccggatccg
     5341 cacatccggg cagtaaacct gcccagccgc ccggagcaac cgcgctcgct cctcgaccga
     5401 gattccggcc agcatggact cgtccgcagc cagcgcctcc aggattgccg tggcggcgcg
     5461 cacgcgtttc aaggcgtcgt caccgttggt cggttggccg gctttagccg acccattgat
     5521 ctgttttgat gtctgttgca ttctcgccaa actacgggta acgagaatgg cttttcgccc
     5581 ccggacggag caatcatagt atccaatcgc ggcgcggcgt caagctggct cgctcaccac
     5641 acccgccgca ggaacaccct cgtgccaccg gaaaggggag ctgcccggca aagacgtgcg
     5701 cggccacctg ttagaatcag cgctcggccc gaaaccctaa cgagctcgcg gcggcagcct
     5761 gaaaagcgcg gcgcacctcg ctgtcgtcgg cattcgcgaa gttcgagcgc tgcaccatca
     5821 ggacgtagat gacttgtttc acgggatcaa tccacgcctg cgtgccgtaa gcgccgccat
     5881 ggccgcaggt gccggctgat aacatggcgg tgatgccctg gggttgttgc accacacacc
     5941 agcccaaccc ccagccgttg ccgggcgtga atccggtctt gaggtcaccc gtctggatac
     6001 tggtcatcag tttgacggac gccggcttta gatactgctt cccgtcgagc gtgccctggt
     6061 tgaggatcat gcggcagaat ttggcgtaat ccggggccgt ggagaacaaa ccgccattgg
     6121 gtgcgggata acgatcgtgg tcggtgggcg aatgcccgtt caagaagctg atcggtgcct
     6181 cctgaagcgt tgcctccgtg cgctgatacg tcttggccag tcggggaagc tgctccgaac
     6241 tgagatagaa cgtggtgtcc ttcatgccca gcggatcgag cagccgcttt tgaaggaagg
     6301 cggggaagga ttgtccggaa acgatctcga caatgcgccc cagcgagttg atgcccgact
     6361 ggcagtattg ccatttcgat cccggctcga atttgagcgg cttgcccaca tagaaaggaa
     6421 tgacatccgc cagcgagcgc gcggtcttgt actgctccgg cgtggcttcg ggcatgcccg
     6481 aggtatgggt gagcaaatgc ctcaaggtga gctttgcagg tttgccgtca gccgttttca
     6541 ggtcacccag ctcaggcagg tacttggcca ccggatcgtc caccgacagc ttcccttctt
     6601 cctgcagcat cagcacggcg gtcgcggtga tgggtttgga catcgaggca atccagcaaa
     6661 tggcatcagt ccgcatgggg gccttggcgg cgatatccgc caggccggtg gcttcgagat
     6721 gcagcacctt gtcgcgcgtc gcgatcaacg taaccacccc agccgcctcc tgtttagaga
     6781 tgcactcctg catcgcgccg gaaacgccgg cgaacgaatc agcgccgccg gcttgccccg
     6841 taaccaggaa caaggcaagc gccgaaagga ttggcacccc aaaggagagg gagagcacgg
     6901 ttgtttgctg agcgttcata cgaggagtat tagacccgga aacaagccgc cagggaagcc
     6961 tgaatgacgg gaagcactac gcggccgtgc ccggctggtt cgtcccaagt gatcacttat
     7021 tcagcaaccc ctggacttcg agccactcgg caaagcgctc gacgcccggc gcggcaggcg
     7081 agacgcccgc cccactattg ccgttaagat ttgccgaacg ccttgggaaa gggcaggttt
     7141 gggtaagtca ggggcgccaa tcttgagcag ccggcgcccc ggcgagccgc agacacgaac
     7201 agaaagacac actatggccc aggcaatcat ggatcccgaa caggtgcggc gtttcgccga
     7261 ggaactgaag cggttcaata ccgacctcaa ggacaagatc gtgtcgttgc aggcgcggtt
     7321 tgcgggattg ggggacacgt ggcaggacca ggagcacatc aaattttcag aggagttcaa
     7381 gcagaccatg aaggcgctca agaagttcgt tgagatctcc aaccagcaca cgccgtatct
     7441 cctgagaaaa gcccagcgca tcgaggaata cctgaaccag cgttagcgct atgcccgaac
     7501 gcgcccatgt cacgtcagtt gatgcgttgg agtccttccg ctccaacctg atcgtctatc
     7561 tgagcaacgc gcgtccgacg ttggaggggg tcagcgccgc ggtccagcgg acgcgcggat
     7621 ggctggaagg cgagcagcgg acgtattggg agagcgagtt ccggcggcgg agccgggcct
     7681 tccaagaggc ccaggcggcg ttgttcagtt ccagaatgtc ccgcatgcgc gaggcgagcg
     7741 cggctgaaca aatggccgtc cagcgtacca agcgggcgct cgacgaagcc gaggcaaagc
     7801 tgagggtggt gaaacagtgg aaccgggtat tcgacaacca agtggacccg ctggtcaaac
     7861 agatggagaa gctgcatacg gttctggccc atgacatggt caaggcggtg gcttttctgg
     7921 cgcaagcgat ccaaacgctg gacgcctatg cggaagtcgc accgcccaca gccgtccctg
     7981 gatcggccca ggctggcggg cagagtgccg agccggggaa caacccatga aggcaagcag
     8041 caaccgactg tcgggcatca ccaaggagct gcgggcccaa tggcaggata cgaaggctta
     8101 ctggaaagac gccaagagcc aggaattcga gcaccgctac atggaagaac tcttggccgg
     8161 cgtggaccgg actgttacag tgatcgaaca actggacaag ctcataacta aaatcaggaa
     8221 ggactgtgag tagcaacctg ggagtcaaca agacgctcga actgctggac cgcctcaaag
     8281 gcacggtccg gaagtttggc gcccgggcgg ataagctgaa tcaggatttc cagggcggca
     8341 ccagccggga acgccgacac cgggaagcct tggttgaaga gcaggcaaag caattgtcgg
     8401 cggccattag cgaggccgag ggagcttttt cggctgccaa gggagcggcg ggggcaaaac
     8461 acgaagcgcg gaaagccagg atcggcaagg cttaccaggc cagcaaagag actgccttgg
     8521 gggatattga gaaccggacg ggctcgcgca agtacgagct ccagaaaagg atgctgcaag
     8581 cggagcgcga ccgggccgcg ggcctaaccg ccgcggccac cacgttcgag gagttcaagg
     8641 caaacctgac ggcagagcag gcagcgctga cgcccctgga aatggctacg cacagcgcat
     8701 tcaagggcta tcggaagctc ctccgcctcc tgacggacgc atacgaaaag gccgccctgc
     8761 cggcggccac gctcgatgag aatcaactgc tggcacagct tcgtgagctg atcgggaaga
     8821 cccgtggcga cttggaacgg tttcagaggt tctggctgct gcgggtgttc aagtatctgg
     8881 cggcctgggt ggtggtcgtc ctttgcgagc ttcccctcgt tctccaccag actggtccta
     8941 attctgccgg ttactggaaa gccggcttgt gcgtggccgg gagcctggtg gtcgtgctga
     9001 ttctccgttc cctcgcccgg agccaggcgg ggccgctcgc cgccgccatt gccggcgcga
     9061 ttgggaaggc gcggcggctg cacgacacgg cccgggaaag gtctgaggct cattaccagc
     9121 aggaactgga gcgcattaaa ggggagtttg agagcacgat ccaaacggtg gatcagcagt
     9181 tgaagcaggc gctggcggag gccggggagc ggcgggtggg ctgtcgcatg cagagcgacg
     9241 agaaggcgtc ccgggctctg gagaagaacg accggctgtg gcaggcaaag cagaaccaac
     9301 tccaacggca acacgcgggc gaccttgagc agttgaagcg cacggcggag acgcgccgga
     9361 aggcggaggt ggaggccagc gaacagaggg aagcgaagct caatacggat tatcatgccc
     9421 agtggcagac gctggaggcg gattggaagc agacgattca gccgatctac gcggcgattg
     9481 agtcggcgaa cgccacagcg gggaagctct ttcctccctg ggagccgcga gtggtggaga
     9541 cctgggctcc gcccgcgcag ttcgcggccg ccgcccaatt cgccaagatg gccgtggacg
     9601 tggagaagct ggcggagacc accatcaagg acaagcggct ggcattgccc ggcccagccc
     9661 gattttccgt gccgctctgc ctggggtatc cggagcaggg ctccatcctg ttcgagaccg
     9721 ccgccgcggg gcacgacgag gccatcggcg cgctcaacaa cgtcatcctg cggctgctct
     9781 cgaccgcgcc gccgggccgg ctgaacttca ccatccttga ccccgtggga ctcgggcaaa
     9841 acttcgccgg tatcatgcac ttggccgact acgaggagca gatcatcaac agccgcatct
     9901 ggacgcagtc cgggcagatc gagcagaagc tggccgacct gaacgagcac atggagaagg
     9961 tcatccagat gtacctgcgc aacgagtatg ccaccatcgc ggagtacaac gagcaggcgg
    10021 gtgtgattgc ggaaaaatac tacttcctcg tcgtggccga cttcccggcg aacttcacgg
    10081 agaccgccgc caagcggctg ctgagcattg cggccagcgg ggcgcgctgc ggcgtgtata
    10141 tgctcattca ctgggaccag cggcagccat tgccgccgga gttcattccc gatgagttga
    10201 gggacagcag cgtttgcctc agcggcaaag ggaaccagtt cctgctgggc ggaaagccga
    10261 tcccggggac ggacctggtc ctcgacgctc cgccgccacc ggagtttgcc atcgagttcg
    10321 tcaacaaggt tggcctggcc agccgggatt ccagccgcgt ggaagtgccc ttcgcccacg
    10381 tggccccatc ggaggcggag atctggaccg aggacacgtc cagcgagctg cgtgtcccgg
    10441 tcggccgcac gggcgcgacc aaattccaat acctcgccat tggcaagggg acttgccagc
    10501 acgcgctgct tgcgggcaag accggttcgg gcaagtccac cctgttccac gtgatcatta
    10561 ccaacctggc gttgtggtgc agccccgagc aggtggagtt ctacctggtg gatttcaaga
    10621 agggcgtgga attcaagtgc tatgcgacgc accgtctccc ccatgcccgg gtggtggcaa
    10681 tcgagagcga ccgcgaattc ggcttgagcg tcttgcagcg ggtggacgat gaactgaggc
    10741 atcgcggcga cctcttccgg cagcttgggg tgcaggatgt cgcgggctac aagcgtgcgg
    10801 gtgggaagga accgatccca cgctcgttac taatcattga cgagtttcag gagttcttcg
    10861 tcgaggacga ccggatttcg cagacggcgt cgctgctgct ggaccggatt gtgcgccagg
    10921 gtcgggcttt cggcatccat gtgctgctgg gctcgcagac cttgggcggc gcttacaccg
    10981 tcgcccgcac caccctcggc cagatggtca tccgcatcgc cttgcagtgc aacgaggcgg
    11041 acgcctacct gatcatggac gagaacaatc ccgcgccccg cctgctctcc cggcccggcg
    11101 agggcatcta caatgacatg gcagggatga tcgaaggcaa cagcccattc caagtggtct
    11161 ggctgccgga tcaggtgcgg gagtcctatc tggccaaggt tcgagcaagg gcggatgcgg
    11221 aagaagtcca aagtctgaag tctaaggtcc aaagtccggg ggcggatggt gggcagcgct
    11281 atccggggcc g
//
LOCUS       DATCGS010000524         8349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_143816, whole genome shotgun sequence.
ACCESSION   DATCGS010000524 DATCGS010000000
VERSION     DATCGS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143816"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VIL39_10740"
     CDS             <1..678
                     /locus_tag="VIL39_10740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY5481949.1"
                     /translation="VRRDWIEENQVFELKSHPPLSRLPSFQQGLFYVQDPSTLLAVRE
                     LDPQPGESVLDLCAAPGGKLTFIAQLMRNEGRLLAHDTTPERLKPIEENCARLGVTCV
                     QTVLPSTLDSQPSTTLDRILIDAPCSNTGVMRRRVDLRWRIRPKEIGRLRATQLELLS
                     RAGSLLKPGGRLVYSTCSLEPEENTDVLTEFLAGHADFRLEHSRELLPFADAVDGTYV
                     ARLVKNG"
     gene            961..1545
                     /locus_tag="VIL39_10745"
     CDS             961..1545
                     /locus_tag="VIL39_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5481950.1"
                     /translation="MMKIQRTIKYDADRVKGFGREVMSVPGCEQLQSCIQCGTCSGVC
                     PLSIYMDYTPRQVMALTRGDFKNEVLRSHTIWLCASCYACTVECPREIRITDIMYELK
                     QRAIHAGIYPKRFPIPVLAREFSEMVKKHGRITEMLLVMKLFLKTNPFAAMGNWRLGI
                     DLLKTGRFSLATEKIKQHGDIARMLDATDAKKES"
     gene            1548..2438
                     /locus_tag="VIL39_10750"
     CDS             1548..2438
                     /locus_tag="VIL39_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEY5481951.1"
                     /translation="MKYSYFPGCSLKGLGRAYEESLLPVMKHLGVELQELDDWNCCGA
                     TAYMAVDEAKACVLAARNLALAEKQGNRPIMAPCAACYLVLNKTKHYFHDYPAMKETI
                     DRALGAVGLRYSGDTPVRHPLDVLINDVGLEAIKQKVRKPLKGLKIATYYGCQLVRPY
                     ATFDDQYNPITMDRLVETLGATVVRYPLKTKCCGGSLTGTVPEAGIRLVYILLNEARK
                     RGADCLATVCPLCQFNLDAYHAQVTAEYGPVLVPTVYFTQLMGLAFGLPERELGLNRA
                     AIPFKWRPTEAPPVKAAAAA"
     gene            2504..4510
                     /locus_tag="VIL39_10755"
     CDS             2504..4510
                     /locus_tag="VIL39_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEY5481952.1"
                     /translation="MKQQANGEIRIGFYICHCGHNIAAMVDCPEVAKYVEKLPGVALS
                     RDYKYMCSDPGQELIQKDIREFKLNRVVVASCSPLLHEHTFRSAVEAGGLNPFFFHMV
                     NIREHDSWVHTDRKEATDKAKALARAAVERVAYHQALEVKKVPIHPDVLVVGGGIAGI
                     HAAMTLANAGKKVFLVEREPSIGGHMAKFDKTFPTLDCAACILTPKMSEVGAHKNITL
                     WSYSEVTKVDGYVGNFTVTVKRKPRYINEDLCTGCQECISNCVYKEPKFLDEFNEGLG
                     KRKPVYIPFPQATPQVVLIDPEVCLNFKHGVLIDKCKKTCVEACGEKKAIDFKQTEEI
                     KEIKVGTIIVATGFQTFDAKRVPQYGYGIYPNVYTSLEVERLVNASGPTGGDVLLRDG
                     RHPKAIGIIHCVGSRDKKTNRWCSRVCCMYSLKLAHLLKEHTDAEIFNFYIDMRTPGK
                     SYEEFYDKLLEEGVQFIRGRVGEVTDWAMTPEEEGKLVLRVEDTLAGFVRRIPVDMVV
                     LATGLEPQADAQAVRRMFNISCGSEGFFLERHPKLAPVSTFTDGIYLAGACQGPRDIP
                     DTVAQAGAAAAEALVLIDRGYIEQEPNTAYVLEEECSGCKSCIPLCPYTAITFDEAKE
                     KATINEALCKGCGTCVASCPSGSIAQRLFEDDEIFSEIHGVLVS"
     gene            4530..5099
                     /locus_tag="VIL39_10760"
     CDS             4530..5099
                     /locus_tag="VIL39_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEY5481953.1"
                     /translation="MSSTTTKPTSPPAPAVVPWQPRIVAFFCNWCTYTAADLAGVSRL
                     KYASNIRVIRLMCSGRVDPQFILDALARGADGVLIGGCHPGDCHYAEGNYKMLRRFQM
                     LKRMLNDLGIEEQRVRLEWISAAEGEKVKRVVNEMVGQVQKLGRLGIPQKFEEWDKEM
                     EHFAAQVAEKAATSPCACAAPEQPALATA"
     gene            5247..6239
                     /locus_tag="VIL39_10765"
     CDS             5247..6239
                     /locus_tag="VIL39_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY5481954.1"
                     /translation="MSTSSKPKIALYWCASCGGCEETVVDLAEDILGVVAAVDIVLWP
                     VAMDFKYKDLEAMPDKSITATLLNGAIRSTEQEEMARLLRRKSQFLIAYGSCAVSGGI
                     PGLANQFSREQILKFNYEDAPTLGNGGPKIRPQLKFAEAGREVSLPELHNVVRSLDQV
                     VEVDYYVPGCPPTPNLTKAAITALLEGKLPPKGSVLAPDIALCDECPRKKSKPTNVSF
                     TEFKRPHQLLADPELCFLAQGVVCMGPATRSGCGSQCTTGNMPCTGCFGPTSRVRDQG
                     LKIMSSLCANVAPKDEGDIDKVLAGIPDPIGTFYRYGLAKSMLRRKVNLTTPSA"
     gene            6813..8342
                     /locus_tag="VIL39_10770"
     CDS             6813..8342
                     /locus_tag="VIL39_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5481955.1"
                     /translation="MNPATQQAFDQGNLRANAAYQSVRRNVTINPITRLEGHGKIDIF
                     LDDKGDVERAYLQVPELRGFEVFSLGRPAEDMPQITSRICGVCPTAHHMAATKALDAL
                     YQVEPTPAGRKIRELVYNTFMLEDHALHVFVLGGPDFIVGPEAPPEQRNIVGVIQKVG
                     LEAGKKVISSRRRLRELIAYFGGKVVHPVLGLPGGVSKALKAEDLPRFKQLAQDGLEF
                     ALWTLDVFKQIVLANPDYVKMITSDAYTHKTCYMGMVDDQNKVNFYDGKLRVVDCDGK
                     EIYKFGPQQYLDYVAEHTEPWSYMKFTYLKPRGWKGFVEGPDTSIYSVAPLARLNASD
                     GMATPKAQAAYEEFYKTLGGKPVHHTLANHWARVVEMIYAAERMVQLVNDPEITSPEV
                     RRVPTNVPKVGIGVVEAPRGTLFHHYETDERGLITMANLVVATANNAARIAMSVEKAA
                     KGLIKGGNVTEGLLNKVEMAFRAYDPCLGCATHSLPGHLPLRVNIYDRQRTLVRQLVQ
                     E"
BASE COUNT         1688 a         2739 c         2419 g         1503 t
ORIGIN      
        1 gtccgccggg actggatcga ggagaaccag gtctttgagc tgaagtcgca tccgccgctc
       61 agccgcttgc ccagctttca acagggtttg ttttacgtcc aagaccccag caccctgctg
      121 gcggtgcgcg aactcgatcc gcagcccggt gaaagcgtgc tcgacctctg cgccgcgccc
      181 ggcggaaaac tcaccttcat cgcccaattg atgcgcaacg aaggccgcct cctcgcccac
      241 gataccaccc ccgaacgcct gaaacccatc gaggagaact gcgcccgcct gggcgtcacc
      301 tgtgttcaga ccgtcttacc ctcaaccctc gactctcaac cctcaaccac tctcgaccgc
      361 atcctgattg atgctccatg ttccaacacc ggggtcatgc ggcgtcgcgt tgatttgcgc
      421 tggcggattc gtccaaaaga gatcgggcgt ctgcgcgcga cgcagctcga gctgctgagt
      481 cgggcgggct cgctgctgaa acccggcggc cggctggttt acagcacctg cagcctcgag
      541 ccggaggaga acaccgatgt gctgaccgag ttcctggctg ggcacgccga tttcagactc
      601 gaacactccc gcgaattgct gccctttgcg gacgctgttg atgggactta cgtggctcgg
      661 ctggtgaaga acggctaagc cgcttcaaac cctgcgcgcc gccgtaaagc tgcgctccgc
      721 cgccgggcca tcggggagac actgtccccc tctgtctcgt acaccaaaat gaacaggtat
      781 caatatgatt agggttactg aaacaatgag gtagtttccg cacaaaaaat ggagagggtt
      841 tggcaaggca ggttgagagt gttcctgcga attatcgtta agtgtaataa tacataacga
      901 aatgtcgtca gtctcccggc aggagacaac gggagctgaa ccggttcgac ggagactctt
      961 atgatgaaaa tacagcgcac gattaaatac gatgcggacc gggtcaaagg cttcggtcga
     1021 gaggtcatga gtgttccagg gtgcgagcag ttgcaaagct gcatccagtg cggcacctgt
     1081 tccggtgtat gccccctgag catctacatg gattacacgc cccgccaggt catggcgctc
     1141 acccgcggcg acttcaagaa cgaggtcctc aggagccaca ccatctggct ctgcgcctcc
     1201 tgttacgcct gcaccgtgga atgcccgcgc gagatccgca ttacggacat catgtatgag
     1261 ttgaagcagc gggccatcca cgcgggaatt tatccgaagc ggttcccgat tcccgtgctg
     1321 gcgcgcgagt tcagcgagat ggtgaaaaag cacggtcgca ttaccgagat gctgctggtc
     1381 atgaagctgt ttctcaagac caaccccttc gcggcgatgg gcaattggcg cttgggaatt
     1441 gacctgctaa agaccggccg gttttcgctg gccacggaga agattaagca gcacggagat
     1501 attgcccgca tgctggacgc caccgacgcc aagaaagaat cctgaacatg aaatactcat
     1561 acttccccgg ttgttcacta aaagggctgg gccgcgccta cgaagaatcg ttgctgcccg
     1621 taatgaagca cctcggcgtt gaactgcagg agttggacga ctggaactgt tgcggcgcga
     1681 ccgcctacat ggcggtggac gaagctaaag cctgcgtgct ggcggctcgt aatctggccc
     1741 tggccgaaaa gcaaggtaat cgtcccatca tggccccctg cgccgcctgc tacctggtcc
     1801 tgaacaagac caagcattat ttccatgact acccggcgat gaaagagacc atagatcgcg
     1861 cgctcggggc agtcgggctg cgctacagcg gcgatacccc ggtgcgtcat ccactggacg
     1921 tcctgatcaa cgacgtcggc ctggaagcca tcaagcaaaa ggtgcgcaaa ccgctcaaag
     1981 gattgaaaat cgccacctac tacggctgcc agctcgttcg cccctatgcg acgttcgacg
     2041 accaatacaa cccgatcacc atggaccggc tggtcgaaac gctcggcgcc accgtcgtcc
     2101 ggtacccgct gaagaccaaa tgttgcggcg gcagcctgac tggcacggtg cccgaagcgg
     2161 gcatccgcct ggtctatatt ctcctcaacg aagccaggaa gcgcggggcc gattgcctcg
     2221 cgaccgtgtg ccctctctgc cagttcaacc tcgacgccta tcatgcccag gtgacggcgg
     2281 agtacgggcc ggtgctggtg cccaccgtct atttcacgca actcatgggc ctggccttcg
     2341 gcctgccgga gcgggagttg ggtttgaatc gggccgctat tccgttcaag tggcggccga
     2401 ccgaggcgcc cccggttaag gcggctgcgg cggcctgatg aggaatcggt cggccgaagc
     2461 ttgaagccaa agaactccga acctcgaatt cctttatctg aagatgaaac agcaagctaa
     2521 tggtgagatt cgcatcggtt tctacatctg ccattgcggc cacaatattg ccgcaatggt
     2581 ggactgtccc gaggttgcga aatacgtcga gaagctgccg ggcgttgccc tttcccgcga
     2641 ctacaagtac atgtgctcgg acccgggcca ggagctcatc cagaaggaca tccgcgagtt
     2701 caagctcaac cgggtcgtcg ttgcctcctg ttccccactc ctgcacgagc acacgttccg
     2761 cagcgcggtc gaggccggtg gcttgaatcc gttcttcttc cacatggtca acatccgcga
     2821 gcacgattcc tgggtgcata ccgaccgcaa ggaggctacc gacaaggcca aggcactcgc
     2881 gcgcgccgcg gtcgaacgcg tggcgtatca ccaggcgctc gaggtgaaga aagtgccgat
     2941 ccatcccgac gtgctggtgg tcggtggcgg catcgcgggc attcacgcgg cgatgaccct
     3001 cgccaacgcc ggcaagaagg tcttcctggt cgagcgcgag ccctccatcg gcggccacat
     3061 ggcgaagttc gacaaaacct tccccacgct cgactgcgcg gcctgcatcc tgacgcccaa
     3121 gatgtccgaa gtcggcgcgc acaaaaacat caccctctgg agctattccg aagtcaccaa
     3181 ggtggacggc tacgtcggca acttcaccgt gaccgtcaaa cgcaagcccc gctacatcaa
     3241 cgaggacctt tgcaccggct gccaggagtg catctcgaac tgcgtctata aggagccgaa
     3301 gttcctggac gaattcaacg agggcctcgg caagcgcaag cccgtctata tcccgtttcc
     3361 gcaagccaca ccccaggtgg tgctcattga tcccgaggtc tgcctcaact tcaagcacgg
     3421 ggtgctcatt gataagtgca aaaagacctg cgtcgaggcc tgcggcgaga agaaggccat
     3481 tgacttcaag caaacggagg agatcaagga aatcaaggtc ggcacgatca ttgtggccac
     3541 cgggttccag accttcgacg ccaagcgcgt tccgcagtac ggctacggca tctacccgaa
     3601 cgtctatacc tccctcgagg tcgagcgcct cgtcaatgcc tccggtccga cgggcggcga
     3661 cgtattgctc cgcgatggca ggcatccgaa agcgatcggc atcatccact gcgtcggctc
     3721 acgcgataag aagaccaacc gctggtgctc gcgcgtctgc tgcatgtatt ccttgaagct
     3781 ggcgcacctg ctcaaggagc acaccgacgc cgagatattc aacttctaca ttgatatgcg
     3841 cacgccgggc aagagctacg aggagttcta cgacaagctg ctcgaagaag gcgtgcagtt
     3901 catccgcggc cgcgtcggcg aagtcaccga ctgggccatg accccggagg aggaaggcaa
     3961 gctggtcctg cgcgtcgagg acacgctggc cgggtttgtc cggcgcatcc ccgtggatat
     4021 ggtggtgctg gccaccgggc tggagccgca agcggacgcg caggccgtgc ggcgcatgtt
     4081 caatataagc tgcggctcgg aagggttctt cctggagcgg catccgaagc tggccccggt
     4141 gagcaccttc accgatggca tttacctcgc cggcgcctgc cagggcccgc gcgacattcc
     4201 cgatacggtc gcccaggccg gcgccgccgc cgccgaggcg ctggtgctga ttgacagggg
     4261 ctacatcgag caggagccga acacggccta cgtgctggaa gaagagtgct ccggatgcaa
     4321 gtcctgcatc ccgctctgcc catacacggc catcacgttc gacgaggcga aggagaaggc
     4381 gaccatcaac gaggcgctct gcaagggttg cggcacctgc gtcgcttcct gcccgtcggg
     4441 ctcaatcgcg cagcgccttt tcgaagacga cgagatattt agcgagattc acggcgtgct
     4501 ggtgtcctag aaatccgaaa gagaacaaca tgagttcaac gactaccaaa ccaacctccc
     4561 cacccgcgcc tgccgtcgtc ccttggcagc cccgcatcgt tgccttcttc tgcaactggt
     4621 gcacctatac agcagcggac ctcgccggcg tatcgcggct gaagtacgcc tccaacatcc
     4681 gcgtcatccg gctcatgtgc tccgggcggg ttgatccgca gttcatcctg gatgcgctgg
     4741 cccgcggcgc ggacggcgtg ctcatcggcg gctgccatcc cggggattgc cattacgcgg
     4801 aaggcaacta caagatgctc cgccgcttcc agatgttgaa gcggatgctc aatgacctgg
     4861 gcatcgaaga gcaacgcgtg cgcttggaat ggatttctgc cgccgagggc gagaaggtca
     4921 agcgagtagt gaacgagatg gttggccagg tccagaagct gggccggctg ggcatcccac
     4981 agaagttcga ggagtgggac aaggaaatgg aacactttgc cgcgcaagtg gcagagaaag
     5041 ccgccacctc cccctgcgcc tgcgccgcgc cagagcagcc agccctcgcc acggcctaat
     5101 cacgtgaacc cggcaccctt agctccaagc tccgcgcctg gctcaggcat tcggatttcg
     5161 accttcggat ttctttcgga tttcgggctt cggatctcgg atttccatgc cctccgtctc
     5221 ccgaacccgc ctgcactctc aatcttatga gcacgtcctc aaaacccaaa atcgccctct
     5281 attggtgcgc ctcgtgcgga ggctgcgagg aaaccgtcgt tgatctggcc gaggacatcc
     5341 tcggggtcgt tgcggcggtg gacatcgttc tctggcctgt ggcgatggac ttcaaataca
     5401 aagacctcga ggcgatgccc gacaagtcca tcaccgccac cctcctcaac ggcgccatcc
     5461 ggtccaccga gcaggaggaa atggcccgcc tgctgcgtcg gaagagccag ttcctcatcg
     5521 cctacggctc ctgcgccgtc agcggcggca ttcccggcct ggccaaccag ttctcgcgcg
     5581 agcaaatcct gaagttcaac tacgaagacg cgccgacgct cgggaatggc gggcccaaga
     5641 ttcgccccca gttgaagttc gccgaggccg gccgcgaagt ctcgctgccc gagcttcaca
     5701 atgtcgtccg ctcgctcgac caggtggtgg aggttgatta ctacgtgccg ggctgtcctc
     5761 ccacgcccaa cttgacgaaa gccgccatta cggcactcct cgagggcaag ctgccgccca
     5821 aaggctccgt cctggcgccc gacatcgccc tgtgcgacga gtgcccgcgc aagaagtcca
     5881 agcccacaaa cgtcagcttc accgaattca aacgcccgca ccaattgctg gcggatcccg
     5941 aactctgctt cctggcgcaa ggcgtcgtct gcatggggcc ggcgacccgc agcggttgcg
     6001 gctcccaatg caccactggc aacatgccgt gcactggctg tttcggcccc acctcgcggg
     6061 tgcgcgacca ggggctcaag attatgtcct ccctctgcgc caacgtcgcg ccgaaggacg
     6121 aaggcgacat tgacaaggtg ctggccggca tcccggaccc catcggcacc ttctaccgtt
     6181 acggcctcgc caagagcatg ctgcggcgga aggtaaacct gaccacaccc agtgcctgac
     6241 ccccttgtga cgcccagcga cacatccaga tgccgtttgc agggtgagtt ccgttccccc
     6301 tcaccccggc cctctcccca agggagaggg agccacgagg ccagcggctc gatccttcca
     6361 ggcgtaacag attcacggac gtgctgccga cgattctccc tctcccttag ggctgtgcat
     6421 tccccacaag tcctctactc cgtcccccaa tcccgaaggg attgtgtcct ccagcccagg
     6481 gttgcgagca gcgagctacc ctgggtgaac ggggtcggga ggtgccaacc ccaccggggt
     6541 tgcggcctcc gttccccgct ctgccgtcgc aaccctgttg gggttgatct cgctcgggtg
     6601 ccttttccca gggtagctcg ctgctcgcaa ccctgggctt tgagacggaa tcccttcggg
     6661 attcgcctgc tcgacgatgc gagttgtgga gaatgcacag ccctgaggga gtgggctggg
     6721 gtgaagggga accgcgctac cgaatcacca accgggcttg tccttgcctt ggacacccgc
     6781 acaaatgtct gttcctagac gagacccaat ttatgaatcc cgccacccaa caagctttcg
     6841 accaaggcaa cctccgcgcc aacgccgcct accagtcggt ccgccgcaac gtcaccatca
     6901 atccgatcac ccgactcgag ggtcacggca agattgacat cttcctcgat gacaagggcg
     6961 acgtcgaacg cgcctacctg caggtgcccg agctgcgcgg cttcgaggtg ttctcccttg
     7021 gccgtccggc ggaggacatg ccgcagatca ccagccgcat ctgcggcgtc tgccccaccg
     7081 cgcatcatat ggctgccacc aaggccctcg acgccctcta ccaggtcgag cccaccccgg
     7141 cgggccgcaa gattcgcgaa ctggtctata acaccttcat gctagaagat catgccctgc
     7201 atgtcttcgt gctgggcggg cccgacttca tcgtcggccc cgaggcgccg cccgagcaac
     7261 gcaacatcgt cggcgtcatc cagaaggtcg gcctcgaagc cggcaagaaa gtcatcagca
     7321 gccgccgacg cctgcgcgag ttgattgcct atttcggtgg caaagtcgtc catcccgtgc
     7381 tcggtctgcc cggcggcgtc agcaaggccc tcaaggcgga ggacctgccc cggttcaagc
     7441 agctcgccca ggacggcctc gagtttgccc tctggacgct cgacgtgttc aagcagatcg
     7501 tcctcgccaa ccccgactac gtgaagatga tcaccagcga cgcctatact cacaagacct
     7561 gctacatggg gatggtggac gaccagaaca aggtcaactt ctatgacggc aagcttcgcg
     7621 tcgtggactg cgacggcaag gagatttaca agttcggccc gcagcagtat ctcgactacg
     7681 ttgccgagca caccgagccg tggagctaca tgaagttcac ctacctcaag ccgcgcggct
     7741 ggaaaggctt tgtcgaaggc ccggacacca gcatctactc cgttgctccg ctggcgcgcc
     7801 tgaacgcctc cgacggcatg gccaccccca aagcccaggc ggcctacgag gaattctaca
     7861 agaccctggg cggcaagccc gtgcaccaca cgctggctaa ccactgggcg cgcgtcgtcg
     7921 agatgatcta tgccgccgaa cgcatggtgc agctcgtcaa cgaccccgag attaccagtc
     7981 ccgaagtgcg gcgggtcccg accaatgtgc ccaaagtcgg cattggcgtc gtggaagccc
     8041 cgcgcggcac cctcttccac cattacgaaa ccgacgagcg cggcctgatc acgatggcca
     8101 acctggtggt cgccacggcg aacaacgccg cccgcattgc catgagcgtg gaaaaggcgg
     8161 ccaagggtct gatcaagggc ggcaacgtca ccgagggcct gctcaacaag gtcgagatgg
     8221 ccttccgggc ctacgacccc tgcctcggct gcgcgacgca ctcgctccca ggccacctgc
     8281 cgctgcgcgt gaacatctat gaccgccagc gcaccctggt ccgccagctc gtccaggagt
     8341 gagccagga
//
LOCUS       DATCGS010000525         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_143973, whole genome shotgun sequence.
ACCESSION   DATCGS010000525 DATCGS010000000
VERSION     DATCGS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143973"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VIL39_10775"
     CDS             complement(<1..1661)
                     /locus_tag="VIL39_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5481956.1"
                     /translation="MKTDPTTPSIPNVQTRLPLKGWALELARRWNGGTYSLFILHGNI
                     FDLFPVQDNSHISYVPLKTFLVRRLFPERACLLFYDISDGLTFGSADMQKRFFEWLEV
                     YDNVENTNFHASGPPRDLLHLAPLLRRFFLRVAEEKEKWRGITLVIDFPEKLIPACEE
                     ANASMEERMNLVTFLKWAAAPELARLDIGVVLVTESADELSAQLLQNPYVAQVRIELP
                     DADDRLRFLQSGWAEEMADGKPSPEWSDFNAEELAARTSGLNLLRIRHFLAEAIRNGI
                     RVTQEHLTASKKRLIEEYCQGLVRFKDPKPGVTLDLVATHTAAKKRLRELAWLIKNGK
                     TDVIERGVLVPGRVGVGKSFLIDCFASECALPVMEMGEFRSKWVGDTERQQARILLTI
                     RALGPVIVVVDEADAVFGTREADGDSGVSGRVFAAFAAHIGDAALRGRELWVAMTSRP
                     DLLSIDMKRQGRFGLCIPLFPAQGADDVLELFTTVAKVKRISLTEPILAFIRQNLGSL
                     PLTGSDVESILIRAKERAVLAKRDEDVQRPDLEEAVDSFIDPLDP"
     gene            complement(1963..>2147)
                     /locus_tag="VIL39_10780"
     CDS             complement(1963..>2147)
                     /locus_tag="VIL39_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481957.1"
                     /translation="SEMDEFKDLVGDMPSTELRVGYQATAMPLPTASTVAAGAPPIIE
                     GVPFKKTYPARQRQKG"
BASE COUNT          398 a          631 c          689 g          429 t
ORIGIN      
        1 tccgggtcga gcgggtcaat gaaggagtcc acggcttcct ccagatcggg acgctggaca
       61 tcctcgtccc gtttcgccag cacggctcgt tctttggcgc gaatcaggat tgattccacg
      121 tcgctgccgg tcaacggcag cgagccgagg ttctggcgga tgaaggcaag gattggctca
      181 gtgagggaga tccttttcac tttcgccacg gtggtgaaga gttcgagcac gtcgtcggcg
      241 ccctgagctg ggaacagcgg gatgcaaagg ccaaagcggc cctggcgttt catgtcaatg
      301 gacagcaggt ccggccggga ggtcatcgcc acccagagtt cgcgcccgcg caaggcggca
      361 tccccgatgt gcgcggcgaa ggcggcgaat acgcgcccgg agacgccgct gtcgccgtcg
      421 gcttcgcggg tgccgaacac ggcgtcggct tcgtccacga ccacaatgac cggccccagg
      481 gcgcgaatgg tcaggagaat ccgcgcctgc tgccgttcgg tgtcgccgac ccatttggag
      541 cggaactcgc ccatttccat gaccggcaag gcgcattcac tggcaaagca gtcaatgagg
      601 aaggacttgc cgacgccgac gcgacctggg acgaggacac cgcgttcaat cacatcggtc
      661 ttgccgttct tgatgagcca ggccagttca cgcagccgct tcttggcggc ggtatgcgtg
      721 gccacgaggt caagagtcac accgggcttc gggtctttga agcgcaccaa accctggcag
      781 tattcctcga tgagccgctt cttgctggcg gtcagatgtt cctgggtgac gcggatgcca
      841 ttgcggatgg cctcggccag gaagtggcga atgcgcagca ggttcagccc cgaggtgcgc
      901 gccgccagct cctcggcgtt gaaatcactc cattcagggg atggcttgcc gtccgccatc
      961 tcttcggccc agccggattg gaggaaacgg agccggtcgt cggcatcagg caactcgatg
     1021 cgcacctggg ccacatacgg gttctggagc aattgggcgc tgagttcgtc ggcagattca
     1081 gtgaccagca cgacaccgat gtccagccgc gcgagttccg gcgccgccgc ccacttgagg
     1141 aacgtcacca ggttcatccg ctcctccatg ctggcgttgg cttcctcgca ggcggggatc
     1201 agcttctccg ggaaatcaat caccagggtg atgccgcgcc acttctcttt ctcctctgcg
     1261 acgcgcagga agaacctacg gagcaggggc gccaggtgaa gcaggtctcg cggcggacca
     1321 gaggcatgga agttcgtgtt ctcgacgttg tcatagactt ccagccactc aaagaaccgc
     1381 ttctgcatgt cagcggaacc gaacgtcaat ccgtcgctga tatcgtagaa cagaaggcag
     1441 gcgcgctctg ggaacagccg ccgcaccagg aacgtcttaa gcggcacata gctgatgtgc
     1501 gaattgtcct gcaccggaaa taggtcaaag atgttgccgt ggaggatgaa gagcgagtag
     1561 gtgccgccgt tccagcggcg cgcgagttca agagcccagc ccttcagggg caggcgggtc
     1621 tggacattgg ggatagaggg agtcgtgggg tcagttttca tggcgggtcc aataaggttt
     1681 ggagtccgaa tattccgaaa cgaatttcat gcttgatctc ataaggccgc agggccatat
     1741 tcttgttggc cagcaggacc gcgacttggc ctcaatgccg ggtctgcaat gtaaggactg
     1801 ctggctttgg ttttccctag ctgcggttgc ctaaccggct caaaagtccg catgatctgg
     1861 tcggctcgct cgtgcatttc cttcggcgtt cccgtccagt gctcgtcggg gattctcacc
     1921 ggctgatttg tctggaggtc atggcatgag tccatcgcgg gcttatcctt tttgccgctg
     1981 gcgcgctgga taggtcttct tgaagggcac gccttcgatg atcggcggcg cccctgcggc
     2041 cacagtggat gcggtgggta ggggcatggc cgtcgcctgg taacccacac ggagttccgt
     2101 ggaaggcatg tcgcccacga gatccttgaa ctcatccatc tcggaga
//
LOCUS       DATCGS010000526         4435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_389722, whole genome shotgun sequence.
ACCESSION   DATCGS010000526 DATCGS010000000
VERSION     DATCGS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389722"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1111
                     /gene="trpS"
                     /locus_tag="VIL39_10785"
     CDS             146..1111
                     /gene="trpS"
                     /locus_tag="VIL39_10785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY5481958.1"
                     /translation="MRILSGIQPSGTLHLGNYFGMMKPAIELQTKGQAFYFIANYHSM
                     TTLVDPVERRQNTLNVALDFLACGLDPQKTVFFRQSDVPEVTELTWLLGAVTPMGLLE
                     RCHSYKDKLAKGISFNFGLFAYPVLMAADILLYDSNVVPVGQDQKQHVEVTRDIAIKF
                     NEQYGETFVIPEPQIRADVAKVPGLDGEKMSKSYGNAIDIFGDEKALRKKMMSIKMDS
                     RTPQEPKPDADQNLAIQILKLVAPPDAAKDYEDRLRAGGLGYGDLKKALFEHYWNYFA
                     TARAKRAELAANLDYVNQVLADGATQARTVAQQVLKRAKVASGLE"
     gene            complement(1115..1888)
                     /locus_tag="VIL39_10790"
     CDS             complement(1115..1888)
                     /locus_tag="VIL39_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5481959.1"
                     /translation="MRNTTQLLTAAALLALTACTTTQPAATSSPPPAQMVKQFQFTKT
                     LSAKVNYLLFLPKGYNAKPGQHWPLILFLHGAGERGTDIWKVATHGPPKNVTAHPDFP
                     FIVVSPQCPERQTWSKDVLLGLLDEVTSQYAVDTNRIYLTGLSMGGYGTWDLGLTYPE
                     RFAAIVPICGGGQMISVILSGSDKGQALKTLGVWAFHGGKDPVVPLEESQRMVNALKK
                     VGVQDVKLTVYPEAGHNSWTEAYNDPQLYEWLLRHDRRK"
     gene            complement(1978..2949)
                     /locus_tag="VIL39_10795"
     CDS             complement(1978..2949)
                     /locus_tag="VIL39_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY5481960.1"
                     /translation="MNHLAQARKVFDIELEALKAVRAQLDGSFAQAVELIVETLRHHG
                     KLIVVGIGKSGHVGHKLAATLTSTGSTSVVLNSVDALHGDLGIVGDGDTVLALSYSGE
                     SEDLLNLLPPLKRFQVKIIAITGAPKSSLARYSDLVLNVRVPKEACPFNLAPTASTTA
                     TLVMGDALAMVVLQARGFKRQDFAKFHPSGAIGRAMLSRVADIMRSGPRNAVAGESLT
                     VQEALLVMTHAKSGSLSVVNGRGKLVGVFTDGDFRRRMATDKNLLAQPLKQVMTRKPI
                     CIRDGALAVEALKVFNERNIDDLIVVNAKHEPVGLVDSQDLPKLKLM"
     gene            complement(2955..4016)
                     /gene="pheA"
                     /locus_tag="VIL39_10800"
     CDS             complement(2955..4016)
                     /gene="pheA"
                     /locus_tag="VIL39_10800"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY5481961.1"
                     /translation="MNIPEHRKAIDKFDTQIVKLLNERTKRVLAIGEIKRKAGEEIYA
                     PHRERAVLQRICRKNHGPITNESLRAVYREIMSSALSLEKSLTVAYLGPEATFTHQAA
                     IRRFGASLRYSPQKTITDVFTEVSKHRADYGVVPVENSTEGVVTHTLDMFVDSDLQIV
                     AQIILPVQHCLLANCPRARIKKLYAHPQAHGQCRAWVQTNLPRVEIIETSSNARSAEL
                     AGKEKGAAAIAGVLASERYDVPVLDYDIQDNVANATRFLVLGRQCSPPTGDDRTSIMY
                     SLVDRVGALYQTLAAFRRYRINMTKIESRPSKRKAWEYFFFVDCEGHMEDRRVAKAIS
                     LLGEHCSYVKVLGSYPDSD"
     gene            complement(4044..>4435)
                     /locus_tag="VIL39_10805"
     CDS             complement(4044..>4435)
                     /locus_tag="VIL39_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY5481962.1"
                     /translation="AYRQPTTRAEVEQVRGVSVDGVMQTLLERGLVEQVGRAEVVGRP
                     MNYGTTALFLEYFGLRSLEDLPAADELRRVVVEKPEALVTADPGLATVPPEQLVLPTE
                     EPKPEGEAAKAEEPAGPEEAPSNATSV"
BASE COUNT          821 a         1387 c         1330 g          897 t
ORIGIN      
        1 ggccgggacg cggcttgtag gccgggtgcc ctcacccggc ggcccaatgt ggtccatagt
       61 gagaattgct gcaggcaccg gtcgcacgat gcccacttgc ggcttgctct ccgccagcat
      121 tcatgccatc taaacactcc tcaagatgcg cattttatcc ggcatacagc cctcgggcac
      181 gttgcacctg ggcaactact tcggcatgat gaagcccgcc atcgagcttc agacgaaggg
      241 ccaggccttc tatttcattg ccaactatca ttcgatgacc acgctggttg atcccgtcga
      301 gcgccgccag aacacgctca acgtggccct cgacttcctc gcttgcggcc tcgaccccca
      361 gaagacggtg ttcttccggc agtcagacgt gcctgaggtg accgaattga cctggctgct
      421 gggcgcggtg actcctatgg gtctgttgga gcgctgccat tcctacaagg acaagcttgc
      481 caaaggcatc tctttcaact tcggcctgtt tgcctacccg gtcctcatgg ctgctgatat
      541 tctgctctac gactcgaacg tggtgccggt tggacaggac cagaagcagc acgtcgaagt
      601 cacgcgcgac atcgccatca agttcaacga gcaatacggc gagacgtttg tcatccccga
      661 accgcaaatc cgcgccgacg tcgccaaggt ccccggcctc gacggggaga agatgagcaa
      721 gagctacggc aatgccattg acatcttcgg cgacgaaaag gcgctccgca aaaagatgat
      781 gagcatcaag atggacagcc gcacgcccca ggaacccaag cccgatgccg atcagaacct
      841 ggccatccaa atcctgaaac tggtcgcccc gcccgacgcg gcgaaggatt atgaggaccg
      901 gttgcgcgcc ggcgggctgg gctacggaga tttgaagaag gctctcttcg aacactactg
      961 gaactacttt gccaccgccc gcgccaagcg cgccgaactg gcggcaaacc tggattacgt
     1021 caaccaggtc ctggccgatg gggccaccca ggcgcgcacc gtcgcgcagc aggttttgaa
     1081 gcgggcgaag gttgccagcg ggttggaata agcctcactt ccgccggtcg tgcctgagca
     1141 gccactcgta gagttgcggg tcattgtaag cctccgtcca tgaattgtgt ccggcttccg
     1201 ggtaaacggt gagttttaca tcctgcacgc ccaccttctt caaggcgttc accatgcgtt
     1261 gggattcttc gagcggcacg accgggtcct tgccgccatg gaacgcccac actccgagcg
     1321 tcttcagcgc ctgccccttg tcactgcccg acagaattac ggagatcatc tggccgccgc
     1381 cgcagatcgg cacgatggcc gcaaacctct ccgggtaagt caacccgaga tcccacgtgc
     1441 cgtagccgcc catgctcagg ccggtcaggt agatccggtt cgtgtccacc gcgtactgcg
     1501 aggtgacctc gtccaaaagc cccagcagca cgtccttcga ccaagtctgg cgttcggggc
     1561 actgcggcga gacgacgata aacgggaaat ccgggtgtgc ggtgacgttc ttgggcgggc
     1621 cgtgcgtggc cactttccaa atgtccgtgc cgcgctcgcc cgcgccgtgc aagaacagga
     1681 tcagcggcca gtgctgccca ggctttgcgt tgtagccttt gggcaggaac aggagatagt
     1741 ttactttggc gctcagcgtc ttggtgaatt ggaattgctt aaccatttgt gcgggcggcg
     1801 ggctcgaggt tgccgcgggt tgggttgtgg tgcatgcggt cagggccagc agggccgccg
     1861 cagtgagaag ttgagtcgtg tttcgcatag gaataggaat catgtttcat tcagttcgaa
     1921 aggagcgcgg ggcattgtca cctgccgcag tccggttggc cgggcgacac gcccggccta
     1981 catcagcttg agcttgggca aatcctgcga atccaccagg ccaaccggct cgtgcttcgc
     2041 gttcaccacg atcaaatcgt caatgttccg ctcattaaat acctttaagg cttccaccgc
     2101 cagcgcgccg tcccgaatgc agattggctt gcgcgtcatg acctgcttca gcggctgcgc
     2161 cagcaggttc ttgtccgtcg ccatgcgccg gcgaaagtct ccatctgtga acacccccac
     2221 cagtttgccg cgtccgttga ccacgctcag gctgccggat ttggcatggg tcatcaccaa
     2281 cagtgcctct tgtactgtca gactttctcc cgccacagca ttgcgcggtc cgctgcgcat
     2341 gatgtccgcc acccgcgaca acatcgcgcg gccaatggcg cctgaagggt ggaatttggc
     2401 gaagtcctgc cgcttaaagc cccgggcctg caacaccacc atcgccagcg catcacccat
     2461 gacgagcgtt gccgtcgtgc tggctgtggg ggcgagattg aacgggcagg cttccttggg
     2521 cacgcgcaca ttcagcacga ggtcgctgta gcgggccaat gaggacttcg gcgcaccggt
     2581 aatggcaata atcttcacct ggaaccgttt cagggggggc agaaggttta gtaaatcctc
     2641 cgattcgccc gagtagctca gcgccaggac cgtatccccg tcgccaacaa tgcccaaatc
     2701 cccatgcagc gcatccacgc tgttcaagac gacactggtg gagccggtgc tggtgagtgt
     2761 ggccgcgagc ttgtgcccca cgtgcccgga cttgccgatg cccaccacga tgagtttgcc
     2821 gtggtggcgc agggtttcaa cgatcagttc gactgcctgg gcaaacgagc cgtcgagttg
     2881 cgcgcgcacc gctttgagcg cctccagttc gatgtcgaac actttccggg cctgggccag
     2941 atgattcata acgttcagtc cgagtcagga taggatccaa gcactttgac gtagctgcag
     3001 tgctcgccca ggagggaaat tgccttggcc acccggcggt cctccatgtg gccctcgcaa
     3061 tcaacgaaga agaagtactc ccacgccttg cgcttgctgg ggcgggattc gatcttggtc
     3121 atgttgatgc gatagcggcg gaaggcggcc agcgtctgat acaatgcccc cacccgatca
     3181 acgaggctgt acatgatgct ggtgcggtcg tcaccggttg gcgggctgca ttggcggccc
     3241 agcaccagga aacgcgtggc attggcgacg ttgtcctgga tgtcgtaatc cagcacgggc
     3301 acgtcgtatc tttcggaggc caggactccc gcgatggcgg cggctccctt ttctttgccc
     3361 gccaactcgg ccgagcgggc attggaggag gtctcgatga tctcgacgcg tggcaggttg
     3421 gtttgcaccc aggcccggca ttgcccgtgt gcttgcggat gcgcatagag cttcttgatc
     3481 cgcgcgcgcg ggcagttggc cagcaggcaa tgctgcaccg gcaaaatgat ctgcgcgacg
     3541 atctgcaagt cgctatcaac gaacatgtcg agggtgtggg tgaccacgcc ctcggtggag
     3601 ttctccaccg ggaccacgcc atagtccgcg cggtgtttgc tgacttcggt gaagacatcg
     3661 gtgatggtct tttgcgggga gtaccgcagg ctggcgccga agcggcggat ggcggcctgg
     3721 tgggtgaaag tcgcttcggg cccaagatac gcaacggtca gggacttctc gagcgacaag
     3781 gcgctggaca tgatttcccg gtaaacagcg cggagcgact cgttggtgat cggcccgtgg
     3841 ttcttccggc aaatccgctg caggaccgcc cgttcgcggt gcggcgcata aatctcctcg
     3901 ccagccttgc gtttgatctc gccgatggcc aggacgcgct tggttcgctc gttcaagagc
     3961 ttgacgattt gcgtgtcgaa cttgtcgatt gccttgcgat gctcggggat gttcatagtt
     4021 ctgtcccttc gggatgatct tgttcaaacg ctagtggcat tggagggcgc ctcttctggc
     4081 ccagccggct cctcggcttt cgcagcctcg ccttcaggtt tcggctcttc ggtcggcaga
     4141 accagttgct ccggcggcac ggtcgccagg ccggggtcag ccgtgaccaa cgcctccggc
     4201 ttttcgacca cgactcggcg cagttcgtcc gcggcgggca gatcctccaa gctgcgcaga
     4261 ccaaaatatt cgaggaacaa cgcggtcgtt ccataattca tcggccggcc caccacctcg
     4321 gcccgcccaa cctgctcgac caacccccgt tcaagcagtg tttgcatcac gccatcaacg
     4381 ctcacacccc gcacctgttc cacctccgcg cgcgtggtcg gctggcgata ggcga
//
LOCUS       DATCGS010000527         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_439670, whole genome shotgun sequence.
ACCESSION   DATCGS010000527 DATCGS010000000
VERSION     DATCGS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439670"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1982)
                     /locus_tag="VIL39_10810"
     CDS             complement(180..1982)
                     /locus_tag="VIL39_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5481963.1"
                     /translation="MSRSIFLLWLALQALSATSIAALPRRLILALDGISFRDMQALQA
                     GVTYKDARGRQFHRQGFDQGYFPVSRNVSTFPSTSDVAWTDVFGDRPLPGYQRTYFSE
                     AANSQIAINGVTTTMEHESQMQWELENGLLRTLGYVYPVHTYKYEVYEFAKAFLNTKS
                     EADNYYVYIRTPDDAQHLSRDHFAMLCALDKKLQELRARYKAQEGRELEILILSDHGS
                     NHAGPAKRIPVRAFLKRAGYRITKSIRDAKDVVLPTAGIETWVEIHNSPPETERLLQL
                     LTHLEGVDVLTARASSQTNRFIVMDAKGGRAIIDWNPAKNSFRYSAERGDPLAYLPVV
                     EALTQKNQFDSDGFATSDAWMAETMGHRYPLALERIVRGHTQVTLNPATILISLDNRY
                     VHASWLIKKGSEFVRFGGTHGGLDDLNSNGILLSSFAPTPDTSTSRVAALFDGFKGLR
                     DYRTEGSGAEWISSQGQALTRIARAPLDRDCRKLPSDGVFLQIWAPSFAHLSSEVPVE
                     VTVEKARRFLPAQIRRGDPKPIDSSEKHWTLSLPLLLPGNCAYERVYARPADLILEPQ
                     AAYRISGRLRDQQKNIRLFNFVFHTDSHGLPAPY"
     gene            2220..2519
                     /locus_tag="VIL39_10815"
     CDS             2220..2519
                     /locus_tag="VIL39_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY5481964.1"
                     /translation="MDNTATLKQDIKRLIVENLMLQITPEEIGDQQMLFGPGGLGLDS
                     VDALQLVVALDKNYGLKIPDPEFARQVLQSTTTMAEAVARHLASPPEAQLSLRPV"
     gene            complement(2595..>3468)
                     /locus_tag="VIL39_10820"
     CDS             complement(2595..>3468)
                     /locus_tag="VIL39_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY5481965.1"
                     /translation="IGTHALVEAGFAPENLGLVIIDEQHKFGVAQREQLVRKGHYPHL
                     LVMTATPIPRTLGLTLYGELDVSTIDALPPGRGSIKTFVRAADKLPKVWAFIRDKLAE
                     GRQAYVVYPRVEESGPNGVKAVTKEFENLQQTLAPFRVGLVHGRLKPRETEQVMAAFR
                     TGQVQVLLATSLIEVGVDVPNATIMLIENAELFGLAQLHQLRGRIGRGAHDSYCILVA
                     AAKSREARTRLQVLEGTTDGFRIAEADLKLRGPGELLGQQQSGMPRFRFGDLAADFEL
                     IQQARELAARWPGA"
BASE COUNT          679 a         1078 c         1025 g          686 t
ORIGIN      
        1 tttctacatg gccggccatt gggtggtggc agggggagga ctgccgtccg ccgcgctcgc
       61 cggcagatat gtggcccaga tgatttgtgc ccgcaacggg aaggtgtttg cggccagcga
      121 gccctaagct gccttccaga gcgccggcct cgcacccggc cggcagagaa gaacccgcct
      181 cagtacggcg ccggcaggcc gtggctgtct gtgtggaaga cgaagttgaa aagccggatg
      241 ttcttctgct gatcgcgaag ccgccccgaa attcggtagg ccgcttgcgg ctccagaatc
      301 agatcggcag gccgcgcata gaccctttcg taagcgcagt tgcctggaag aagaagcgga
      361 agactgaggg tccaatgctt ttcggaggaa tcaatcggtt tgggatctcc ccggcgaatc
      421 tgcgcgggca ggaaacgtcg ggctttctca accgttacct cgactggcac ctcactgcta
      481 aggtgagcaa agctcggcgc ccaaatctgc aggaagacac cgtcgctagg cagcttacgg
      541 caatcccgat cgagcggcgc tcgggcgatc ctcgttaggg cttgtccttg gctggagatc
      601 cattcggcgc cacttccctc cgtgcgataa tcccgtaacc ccttaaatcc gtcaaagagc
      661 gcggccaccc ggctcgtcga ggtgtccggg gtgggggcga aactgctcag caggatgccg
      721 ttggagttga gatcatcgag ccccccatgc gtgcccccaa atctcacaaa ctcgctgccc
      781 ttcttgatca gccacgaggc gtgaacatag cgattgtcca ggctgatgag aatcgtcgcc
      841 ggattgagtg taacctgcgt gtggcctcgg acaattcgct ccagcgccaa cggatagcga
      901 tggcccatgg tttcagccat ccaggcatcg gacgtggcaa agccgtcgga gtcgaattgg
      961 ttcttttggg tgagagcttc gaccaccggg aggtaagcaa gcgggtcgcc gcgctcggca
     1021 gaatatcgga aggagttctt cgcgggattc caatcaatga tagcccggcc gcccttcgca
     1081 tccatgacaa taaaccggtt cgtctgactc gaagcccggg cggtcaacac gtccacgcct
     1141 tccaaatgcg tgagaagctg cagaagcctc tccgtttccg gaggcgaatt atggatttcc
     1201 acccaggtct cgatcccagc cgtagggagg acgacgtcct ttgcatcccg gatggacttc
     1261 gtgatccggt atccggccct tttcagaaac gcccgaaccg gaatgcgttt ggcggggccg
     1321 gcgtggttgc tgccatggtc cgagagaatc aggatttcca gctccctgcc ctcttgcgcc
     1381 ttgtaccgag cgcgcagctc ctggagcttc ttatcgagcg cgcaaagcat ggcgaagtga
     1441 tcgcgcgaca ggtgctgcgc atcatccgga gtgcgaatat agacgtagta gttatcggcc
     1501 tcgctcttgg tgttgagaaa ggctttggcg aattcgtaca cttcgtattt gtacgtgtgg
     1561 accggataga catagcccag cgtgcgaagc aaaccgttct ccaactccca ttgcatctgg
     1621 ctttcgtgct ccatcgtcgt ggtgacgccg ttgatggcaa tctgtgaatt cgccgcctcg
     1681 ctgaaataag tgcgctgata accgggcagc gggcgatcac cgaaaacgtc ggtccacgcg
     1741 acgtcgctcg ttgatggaaa cgtcgagaca ttgcggctga ccgggaagta gccctggtcg
     1801 aaaccctgcc gatggaactg cctgcccctg gcatccttgt aggtgacccc tgcctgcaga
     1861 gcctgcatgt cacggaagga gatgccatca agtgccagaa tcaaacgcct ggggagcgcg
     1921 gcaatgctcg ttgcggagag cgcctggagg gccaaccaga gaagaaagat agaccggctc
     1981 acgtctcttc tcaggcgacc tgaacaaccg aagctccggc gaaaaatcat ggcgcgcact
     2041 tcttaacggg tctggtccgc catgtccagc aggctgttgg aatacggggc gagctaacgg
     2101 tccgacgagc cggatgatgt tttacagccc gcagccggct tttgggtcgg cttttcggct
     2161 tgccccggaa cctgccattt cagatactgt atgcgttaag tcgaagacgg cattttgaaa
     2221 tggacaatac ggcgacactg aaacaggaca ttaagcggct gatagttgaa aacctgatgc
     2281 tgcaaatcac ccctgaggaa attggcgacc agcagatgct gttcggcccc gggggcctcg
     2341 gactggattc agtggacgcc ctccaactgg tcgtggcgct cgataagaac tacgggctca
     2401 agatccccga tcccgagttt gcccgccagg tccttcagag cacaaccacc atggccgaag
     2461 ccgttgcccg gcatcttgcc tcgcctcccg aggcccaact ttcgctccga cccgtttagc
     2521 ctgccttggc caacccggca atccaggtgc tgtcagaagt tcggatttct gacctcggcg
     2581 gggccgccgg ttcctcacgc gccgggccac cgcgccgcca actcccgggc ctgctggatt
     2641 agctcaaagt ctgcggccaa atcgccaaaa cggaatctcg gcattccgct ttgttgctga
     2701 ccgagcaact cgcccggtcc ccgcagtttc agatccgcct ctgcgatacg gaacccatcg
     2761 gtcgtccctt ccagcacctg caagcgcgtg cgcgcctccc ggctcttggc ggcggcgacc
     2821 aggatgcaat aggaatcgtg cgcgccgcgc ccaatccggc cgcgcagttg atgaagctgc
     2881 gccagcccga acagctcggc gttttcgatc agcatgatcg tggcgttggg cacatccaca
     2941 ccgacctcga tcagcgaggt tgccaggagc acctgcactt gcccggtgcg aaacgcagcc
     3001 atgacttgct cggtctctct gggtttcagc cgcccgtgca cgagaccgac gcggaacggc
     3061 gccagcgtct gctgcaggtt ttcgaattcc tttgtgacgg ccttgacccc atttgggccg
     3121 ctctcctcca cccgcggata aaccacatag gcctgccggc cctccgccag tttgtcacgg
     3181 ataaacgccc acaccttcgg cagcttatcg gccgcgcgca cgaacgtttt gatgctccca
     3241 cgaccaggcg ggagcgcgtc gatcgtggac acgtccagct cgccgtacaa cgtcagcccc
     3301 agcgtgcgcg gaatgggcgt cgcggtcatc accagcaggt gcgggtagtg gcccttgcgc
     3361 accagttgct cgcgctgggc cacgccgaat ttgtgctgct cgtcaataat caccaggcca
     3421 aggttctccg gcgcgaaccc agcttcgacc agcgcatgcg tgcctatc
//
LOCUS       DATCGS010000528         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_737832, whole genome shotgun sequence.
ACCESSION   DATCGS010000528 DATCGS010000000
VERSION     DATCGS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737832"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VIL39_10825"
     CDS             <1..539
                     /locus_tag="VIL39_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481966.1"
                     /translation="LAYYRYRVQDEYVLVAQINGELVAVVNGRLLSKEVGMSYHTVAL
                     RRGLRVGAHAFAAKMEYHLDIIGQKEVLIVAESPIGFRRWMIEYELEKRFNMAHELGG
                     VPSWALTRELFNKARETLVVGRRPVPKHLLEKAQAAILPPTDPPKPPADLLAATRSMY
                     DSTGTALMAQRWKQEGRL"
     gene            542..1717
                     /locus_tag="VIL39_10830"
     CDS             542..1717
                     /locus_tag="VIL39_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY5481967.1"
                     /translation="MREVYAVGVGITSFTRLEYPISEIAAYPAMMALKDAGLSKVDHV
                     YVANMGSARLNHQSGLASAVVDTLSLTPAGAEVIENGPASGASAIKTGFMAVASGLHD
                     VVLVTGAERLREVNNLDATDFVATLTHPLAEYIYGVTLPSLAAMFTRLYMQKYGVTER
                     QLAMVAVKNHDNALLNFYAHLHEKITLEGILDSPEAMTNNPYVAEPLRFFDCCPVSDG
                     GACVILASAEVAKKLKKPMVRLAGVGQATDSHCVHERAEPTDLLAVRGAAAQAFKMAG
                     ITPGDVDVAELHDAFTILEIAESEEVGFFKRGQGHLALERGETRLGGKLPINTSGGLK
                     AKGHPVGATGVGQAHEIILQLRGEAEKRQVKDAKIGFTCNFGGFGNNVVCLVFKRED"
     gene            1725..2066
                     /locus_tag="VIL39_10835"
     CDS             1725..2066
                     /locus_tag="VIL39_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5481968.1"
                     /translation="MEIYAYKCKKCGQLHYPYRMVCKKCGKNDVFEFDTVPLPKKGKL
                     LTFTTVHNLPPDFNVAKLGLGIVELENGMRITGQIKITNPKLGMPVTGKVEIVREAEY
                     SKNYGMVFYPA"
BASE COUNT          440 a          692 c          694 g          389 t
ORIGIN      
        1 tgctggccta ttatcgttat cgcgtccagg atgagtatgt tctggtcgcc caaatcaacg
       61 gggagctggt cgcagtagtg aatggccggc tgctgagcaa ggaggtcggc atgtcctacc
      121 acaccgtggc gttgcggcgc gggttgcgcg tcggcgccca cgccttcgcg gccaagatgg
      181 agtatcacct cgatatcatc ggccaaaagg aagtgctcat tgtggccgaa tctcccatcg
      241 ggttccgccg ctggatgatt gaatacgaac tggagaagcg cttcaacatg gcgcacgagc
      301 tgggcggcgt cccctcctgg gcgctgacca gggaactgtt caacaaggcc cgcgagacgc
      361 tcgtcgtcgg ccgccggccg gtgcccaagc atctgctgga aaaagcgcaa gccgccatcc
      421 tgcccccgac tgacccgccc aagccgccgg cggatctcct ggccgctacc cgctccatgt
      481 acgactcgac gggcaccgcg ctcatggctc aacgctggaa gcaggaaggg aggctttgat
      541 tatgcgcgag gtctatgcgg ttggcgtggg tatcaccagc ttcacgcgcc tggaataccc
      601 gatcagcgaa atcgccgctt acccggcgat gatggcgctc aaggacgccg ggctgtcgaa
      661 ggtggaccac gtctatgtgg ccaacatggg cagtgcccgg ctcaaccacc agagcgggct
      721 ggccagcgcg gtggttgata cgctcagtct caccccggcc ggcgccgagg tgatcgagaa
      781 cggccccgcc tccggcgcgt cggccatcaa gacgggtttc atggccgtgg cctccgggct
      841 gcacgacgtc gtgctggtca ccggcgccga gcggttgcgc gaggtgaaca acctcgatgc
      901 gacggacttc gtcgccacgc tcacccatcc cctggcggag tatatctacg gcgtcacgtt
      961 gccgtcgctg gccgccatgt tcacacgcct ctacatgcag aagtacggcg tgaccgagcg
     1021 ccaactggca atggtggccg tgaagaacca cgataacgcg ctgttaaact tctacgcgca
     1081 tctgcacgag aagatcacgc tcgaaggcat cctcgactca cccgaggcca tgaccaacaa
     1141 cccttatgtc gccgagccgc tgcgcttctt cgactgctgc cccgtctcgg acggcggcgc
     1201 gtgtgtcatc ctggcttcgg cggaggtcgc caagaagctg aagaagccca tggtccggct
     1261 ggccggcgtg ggccaggcga cggattcgca ctgcgtccac gagcgtgccg aaccgaccga
     1321 cctgctggcg gtgcgcggcg cggcggccca ggccttcaag atggcgggga tcactccggg
     1381 tgacgtggat gtcgccgagc tgcacgatgc gttcaccatc ctggagattg ccgagagtga
     1441 ggaagtgggt ttcttcaaga ggggccaagg gcacctcgcg ctagagcgcg gcgagacccg
     1501 gctgggaggg aagctgccca tcaacacctc aggcggtctg aaggccaagg gtcatcccgt
     1561 cggcgcgacc ggcgtcggcc aggcccacga gatcatcctg caactccgcg gcgaagccga
     1621 gaagcggcag gtcaaggacg cgaagattgg tttcacctgc aactttggcg gcttcggcaa
     1681 caacgtcgtc tgcctcgtct tcaagaggga ggattaatat ggccttggaa atctacgctt
     1741 ataagtgcaa gaaatgcggg caactgcatt acccctaccg catggtctgc aagaaatgcg
     1801 gcaagaacga cgtgttcgag ttcgacaccg tgccgctccc caaaaaggga aagctgctga
     1861 ccttcaccac cgtccacaat ctgcccccgg atttcaacgt ggccaagctc ggcctcggga
     1921 ttgtggagct ggaaaacggc atgcgcatca ccggccagat caagatcacc aacccgaagc
     1981 tcggcatgcc cgtcaccggc aaagtcgaga tcgtccgcga agccgaatac agcaagaatt
     2041 acgggatggt gttctatccg gcgtgagccg gcggagcgcg gacattcttg tccgccagtt
     2101 ccttgcacgt cgcttggcgg acaagaatgt ccaatgccac taggatttga ccctcctcat
     2161 cccggaggga tggccgagaa tagcccaacg tttcaacgtt gggggcgcga gttta
//
LOCUS       DATCGS010000529         6052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_490958, whole genome shotgun sequence.
ACCESSION   DATCGS010000529 DATCGS010000000
VERSION     DATCGS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490958"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VIL39_10840"
     CDS             <1..1154
                     /locus_tag="VIL39_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5481969.1"
                     /translation="DWDFPGILTDLIGDLTWSPKPIEVKLFSTDIEFLKKKAPEVEAQ
                     LKKVKGVVDTLDGLIYAGPTITLRMRYADAERFGLNAADVVAAVTTAMLGQEASTVLE
                     GDRVVNIRVLVDHASIDRIATLRELPLRAPDGTVVKLSQVADVVEQPGQLELRREDLR
                     QDVAVSARLEGRDLGSAMAEIQEKLSQDKTIPPGTIEFGGLYQQQRESFRNLMLVLAM
                     AIFLVFTVLLIEFRSFYEPVAIVFGAVLALFGTVLALWVTGTSLNVVSFLGAIIGVGI
                     VAKNGILMLDFVEHLRAEGASLEDALVRSGHRRLRPVLMTSLAAAFGMLPLAYGIGSG
                     ADMLRPLAIAVIGALCISVLLSLLATPTVYYLMLRLREKPALQKLAPRQ"
     gene            complement(1306..2538)
                     /locus_tag="VIL39_10845"
     CDS             complement(1306..2538)
                     /locus_tag="VIL39_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481970.1"
                     /translation="MINILLVDDDPLILKVYQERLAQQGAHVTTAADGMAAIQALRAS
                     KPDVAVLDLMMPRFSGVDVLKFIRSQPELKTLPVVVLSNSYMNQLAAEAAALGVQKAL
                     LKVRCSPTVLFEIINDILAGRTSTENSSHLLAVPERLPAAPSVSDIISPGSSQAPPPS
                     EAQSATEFHAKARRSFLQSAPVTCTAMRSLCQAFLSEPKETEREMKLQNFYRKIHFIA
                     ATAGLAQCHHLAQMACAFEALLFELSSRPAAIGPSVSRTIAATVDCLALLFDRARETD
                     DETPLSMQALVVDDDALSNRLVVSALQRAQLQARSADDPLVGLQWLKETRFDLVLLDI
                     EMPGMDGFELCRKLRLLPGYKKTPVIYVTSHSDFDNRARSVLSGGDDLISKPIFPMEL
                     AVKAVTLLLKRKMAGGAP"
     gene            complement(2582..3553)
                     /gene="galE"
                     /locus_tag="VIL39_10850"
     CDS             complement(2582..3553)
                     /gene="galE"
                     /locus_tag="VIL39_10850"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY5481971.1"
                     /translation="MNVFVTGGAGYIGSNCTEALLDAGHQVTVYDNLSEGHRSAVDPR
                     ARFVLGRPDEENNILNAVKAAKPDAIMHFAANALVGESMTNPKKYFHNNVSNGLKVLD
                     AAVECGVKKFVFSSTCATYGPPERLPMTEDLPQRPINPYGESKLMFEKMLQWYQRIYG
                     LEFIAFRYFNAAGASEKFGEHHRVEAHLIPNVLHVALGQKSHCEIYGTDYPTPDGTCI
                     RDYIHILDLAQAHILGLAPSRQGFYNLGNGDGYSVREVIRMCEKVTGKAIPATEKPRR
                     PGDPPRLVAAAERAIQELGWKPKYPKLADIVATAWAWHKAHPKGYLD"
     gene            3743..3931
                     /locus_tag="VIL39_10855"
     CDS             3743..3931
                     /locus_tag="VIL39_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481972.1"
                     /translation="MDKANKMIHQLGTSTAEMIRIFVAQIARTGKVTFKLHAQSDDLL
                     GVERRNQVLRDLDGSEGW"
     gene            3988..5229
                     /locus_tag="VIL39_10860"
     CDS             3988..5229
                     /locus_tag="VIL39_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5481973.1"
                     /translation="MSVVVLLLVVAKWAAQLWLEQLNRRYALAHAAMVPEAFKGFVDD
                     ATYAKSVQYTLAKGRLDQIAVTYNSAVLLLVLLSGVLPWGFHGFAQRVGGSAWAMAAF
                     LFVTAIALSLPGLPLDWYDQFCLEDRFGFNTTTQKLWWMDRLKGLLLGTVLGYPLLVL
                     VLKLVEWTGAWWWLWAWGALLGFQLLMLVLAPVLILPLFNKFTPLPEGSLRERLLKLA
                     EQTCFRARSIQVMDGSKRSRHSNAFFTGFGGFRKIVLFDTLIQQLAEPELAAVLAHEI
                     GHFKKKHIPKTLAAAALSSLAGLYLVSLLAQQEWFYRGFGFEPGSIVPALLLFGLLSS
                     VVTFWFSPVAHWWSRRYEYQADAFAAGIMTEPGSLIGALRKLNEKNLSNLTPHPLYSG
                     FYYSHPTLLEREQALAGALKA"
     gene            complement(5803..>6052)
                     /locus_tag="VIL39_10865"
     CDS             complement(5803..>6052)
                     /locus_tag="VIL39_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481974.1"
                     /translation="HSVGFEPIYRMKTIPNTACKAALAATALALFLALPAHAATFSPN
                     PSADTFVTTGPTANLSGNNYGGVGALSVTASGLTNGEF"
BASE COUNT         1078 a         1773 c         1937 g         1264 t
ORIGIN      
        1 tcgattggga ttttcccggc attctcaccg acctcatcgg cgacctgacg tggtcgccca
       61 agccgattga agtgaagttg ttttccaccg acatcgagtt cctgaaaaag aaggcgccgg
      121 aagtcgaggc gcaactcaag aaggtaaagg gcgtggtgga taccctggat gggctgatct
      181 atgccggccc gacgatcacg ctgcgcatgc gctacgctga tgcagaacgg tttggcctga
      241 acgccgcgga cgtggtggcg gcggtgacca ccgccatgct cggccaggag gcctcgacgg
      301 tgctggaagg ggatcgcgtg gtgaacatcc gcgtcctggt ggaccacgct agcatcgacc
      361 gcatcgccac gctgcgcgag ctgccgctcc gcgcgccgga cggcaccgtg gtgaagctct
      421 cgcaggtcgc cgacgtcgtc gagcagcccg gccagcttga actgcgccgc gaagatctgc
      481 ggcaggacgt ggccgtgagc gcgcgtctcg aaggccgcga cctgggcagc gcgatggcgg
      541 aaatccagga gaagctcagc caggacaaga ccatcccgcc aggcacgatc gagtttggcg
      601 ggctctacca gcagcaacga gagtccttcc gcaacctcat gctggtgctg gcgatggcca
      661 tcttcctggt ttttaccgtg ttgctgattg agttccggtc tttctacgaa ccggtggcca
      721 ttgtcttcgg cgcggtcctc gccttgtttg gaaccgtgct ggcgttatgg gtcaccggca
      781 cttcattgaa tgtcgtttcc ttcctcggcg caatcattgg cgtcggcatc gtggcgaaga
      841 acggcatcct gatgctcgac tttgtcgagc acttgcgggc ggaaggcgcg agtctggagg
      901 atgcgctcgt ccgctccggc catcgccgct tgcgtccggt gctaatgacc tcgctggcgg
      961 cggccttcgg catgttgccc ctggcctatg gcatcggcag cggggcggac atgctgcgcc
     1021 cgctggctat cgcggtgatc ggcgctttgt gcatttccgt gctcttgtcg ctcctcgcca
     1081 cgccgacggt gtattacttg atgttgcgcc tgcgtgaaaa accggcgctg caaaagctgg
     1141 cgccgcggca gtgatgttcc gcgcacttct tgcctggtcc ttcgtggccc aggaacgttt
     1201 gatcctgtcg tggggcaaga ccgtcgcgga ccggaaggac ctgccgtcct ttatccacga
     1261 cctgacctcc ctggaaaccg tgctggttcc tgacaggccc gcccctcagg gcgcaccacc
     1321 cgccatcttg cgtttcagca acagggtcac tgctttgacg gccagttcca tggggaagat
     1381 cggcttggag atcaggtcgt ctccgccgct caggacgctt cgggcgcggt tgtcaaagtc
     1441 ggagtgactg gtgacataga tcactggggt cttcttgtag ccgggcaaca ggcgcagctt
     1501 ccggcacagc tcaaatccat ccatcccggg catttcaatg tccaggagca cgaggtcaaa
     1561 gcgggtttcc ttcagccact gcaagccgac caatggatca tcagcgctgc gggcctggag
     1621 ttgcgcgcgc tggagggctg agacgaccaa tcggttgctc aacgcatcgt cgtccacgac
     1681 cagggcctgc attgacagcg gggtttcgtc gtccgtttcg cgggcgcgat caaagagcag
     1741 ggcaagacaa tcaaccgtgg cagcgatggt gcgcgacacc gatgggccga tggcggccgg
     1801 tctgctgctc agttcgaaca gtaacgcctc gaacgcacag gccatttggg caaggtggtg
     1861 acattgtgcc aggccggctg tcgccgcgat gaagtggatc ttgcgataga agttctggag
     1921 cttcatctcg cgttcggtct ccttcggctc agagaggaag gcctggcaaa ggctgcgcat
     1981 ggccgtgcag gtcacgggcg cgctctggag gaagctccgg cgggctttgg cgtgaaactc
     2041 ggtggcggat tgggcctcac tcggtggcgg cgcctgggaa ctgcccgggc taatgatgtc
     2101 ggacaccgag ggggcagccg gcagtcgctc cggcacggcg agcagatgcg aagagttctc
     2161 agtgctggtt ctgcccgcca gaatgtcatt gataatctcg aacaggacgg tgggtgaaca
     2221 gcgaaccttg aggagcgcct tttgcacgcc aagcgcggca gcctccgcgg ccaactggtt
     2281 catgtaggag ttggacagca ccaccaccgg cagggttttc agctcgggtt gcgagcgaat
     2341 gaatttcaac acgtccacac cgctgaacct gggcatcatg aggtcgagta cggccacatc
     2401 cggcttgctg gcgcgcagag cttgaatcgc ggccatgcca tcggcggcgg tagtgacgtg
     2461 ggccccttgc tgggcaagcc tctcctgata caccttcagt atgagtggat cgtcgtccac
     2521 cagcagaatg tttatcatat acagttgcca gaccgctttc ccaccttgcg gacttgggct
     2581 ttcagtccaa gtagcctttg ggatgggcct tgtgccaggc ccaggcggtg gcgacgatgt
     2641 ctgcaagctt cggatacttg ggcttccacc ccagctcctg aatcgcccgc tcggctgcgg
     2701 ccaccagccg gggcgggtct ccggggcggc gcggcttctc ggtggccgga atcgccttgc
     2761 ctgtcacctt ctcgcacatc cgaatgacct cgcggaccga gtagccgtcg ccgttgccca
     2821 ggttgtagaa accctgccgg ctgggtgcca ggccgaggat gtgcgcctgg gccaggtcga
     2881 ggatgtggat gtaatcgcgg atgcacgtgc cgtcgggcgt gggataatca gtgccgtaaa
     2941 tctcgcaatg tgatttctgg cccagggcaa cgtgcagcac gttcggaatg agatgcgcct
     3001 cgacgcgatg atgctccccg aacttctcgc tcgcgccggc ggcgttgaag tagcggaacg
     3061 cgatgaactc caacccgtag atcctttgat accactggag catcttctcg aacatgagct
     3121 tggactcgcc ataagggttg atggggcgtt gcggcaaatc ttcggtcatc ggcaggcgtt
     3181 cgggcgggcc gtaggtggcg caggtcgagc tgaatacgaa cttcttgacg ccgcattcga
     3241 ccgcggcatc gagcactttg aggccattgc tgacgttgtt gtggaaatac ttcttcgggt
     3301 tggtcattga ttccccgacc agcgcgttgg cggcgaaatg catgatggcg tcaggcttgg
     3361 ccgctttgac cgcattcagg atgttgttct cctcgtccgg ccggccgagt acaaaccgcg
     3421 cccgtgggtc caccgccgaa cggtggccct cagagaggtt gtcataaacg gttacttgat
     3481 gcccggcgtc gagcaacgcc tcagtacaat tcgagccgat atacccggcg ccaccggtga
     3541 cgaagacgtt catggggaac aagctagtcg ttggcggaag gtgactcaac ggaaaaagcc
     3601 gccccacaac cgtgctctct ctgtaaacct cgatgggttt tccccgcccg cgcagggttg
     3661 gtccggcttg acagcgttcg ggtctggggc acaattggag catgatcgtg ctctttcgct
     3721 gtcgcatcga gaaaccgctg ttgtggataa agccaacaag atgattcacc agcttggcac
     3781 ctccactgcc gaaatgattc gcattttcgt ggcgcaaatt gcccgaaccg gcaaggtaac
     3841 gttcaagctc cacgctcagt cagacgacct gcttggcgtg gagcgtagga accaggtatt
     3901 gagggacctc gatggctccg aaggttggtg aagtctagtc tagtccaccg cttggccatc
     3961 cctctttcga tgagtgcgct cagtgtagtg agtgtggtcg ttctgctgct ggtggtcgcc
     4021 aagtgggcgg cccagctttg gctggagcag ttgaaccggc gctatgcgct ggcccatgca
     4081 gccatggtgc cggaggcttt caagggcttc gtggacgacg cgacctacgc caagtccgtg
     4141 caatacacgc tggccaaggg ccgcctcgac cagattgcgg tgacctacaa ttctgcggtg
     4201 ctgctgctgg tgcttctcag cggggtgctg ccgtggggat ttcacgggtt cgcgcagagg
     4261 gtgggcggat cggcctgggc catggccgcc ttcctcttcg tcacggctat tgcattgtcg
     4321 ttgccgggtc tgccactgga ttggtacgac cagttctgcc tggaagaccg cttcggcttc
     4381 aacaccacga cccagaagct ctggtggatg gaccgcctca aagggctgct gctggggacc
     4441 gtgctgggct acccgctgct ggtgctggtg ctcaagctgg tcgagtggac cggggcatgg
     4501 tggtggttgt gggcctgggg cgcgctgctt ggatttcaac tgctgatgct cgtgctcgcc
     4561 ccggtcctga tcctgccgct gttcaacaag ttcacgccgt tacccgaagg cagcctgcgc
     4621 gagcgcctat tgaagctggc cgagcagact tgtttccgcg cccggagcat ccaggtcatg
     4681 gacggcagca aacgctcccg gcactcgaat gcgttcttca ccggcttcgg cggcttccgg
     4741 aagatcgtgc tgttcgacac gctgatacaa caactggccg agccggagct ggctgccgtg
     4801 ctggcgcacg aaatcggcca tttcaagaag aagcacatcc ccaagacatt ggcggccgcg
     4861 gcgctgagct cgctggccgg gctctacctg gtctcgctac tcgcccagca ggaatggttc
     4921 tatcgcggct ttggctttga gccggggagc attgtgcccg cgctgctgct gtttgggctg
     4981 ctgtcgagcg tggtcacgtt ctggttctca ccggtggcgc attggtggtc gcggcgttac
     5041 gaataccagg cggacgcctt cgccgccgga atcatgaccg agccgggctc gctgatcggc
     5101 gcgctgcgca agctgaacga gaagaacctc agcaacctca cgccgcatcc gctctatagc
     5161 gggttctact attctcaccc gacactgctc gaacgtgagc aagcgctggc aggcgcgttg
     5221 aaggcttagg tccggccttt agggatggcg ttttcgcggg cgtgaaccgt gccgcggtgc
     5281 atgctggacg agagcagaaa tacacgctgc ctcgccagct tctcacgccg ggtcagggga
     5341 tccggcctgc aagacagctc gatagttgta ggcccgatac gctcaccgga cggcttacgc
     5401 aacctccatg gggtcatgat ttcagagcct cctcgtcggc ctgattaaga aacccggttc
     5461 ctgcccctga ccaacccatt cccggcccag gcgggcggtc attccacgcc ctctcccgga
     5521 tttcgggtgg cttgtaggtg cctaccgcca ctagtgtacc ttaatagtac cttaatagta
     5581 ccctgatagt aacatagtat cacagccaga ctagtttcag tgcatcacca ttgtgcttgt
     5641 ggattacggc gctacgttaa cgttacctaa gacgtatgag gcgttatttc tagggagaat
     5701 tcggctgtcc aacgagtatt tctccaagag acgacggggt gacctgcaaa tggcacagct
     5761 cggccttcct ggcctttacc ggcaggcggg tcgcagaagg ctctagaact cgccgttggt
     5821 cagacccgaa gcagtcacgg agagggctcc gacgccaccg tagttattgc cgctgagatt
     5881 ggcggtcggg ccggtggtga cgaaggtgtc ggcagaggga ttgggagaga aagtggccgc
     5941 atgagccggg agcgcgagga agagggcgag tgcggtggcc gcaagggcgg ctttgcaggc
     6001 tgtgttcggg attgtcttca tacgatatat aggctcaaat ccaacggaat gc
//
LOCUS       DATCGS010000530         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_291889, whole genome shotgun sequence.
ACCESSION   DATCGS010000530 DATCGS010000000
VERSION     DATCGS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291889"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /gene="purS"
                     /locus_tag="VIL39_10870"
     CDS             complement(<1..165)
                     /gene="purS"
                     /locus_tag="VIL39_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY5481975.1"
                     /translation="MKAKIVITPKKAVLDPQGKTVQNALAQMGYTGVGAVHVGKYLEI
                     EVAGADKEAAR"
     gene            323..733
                     /locus_tag="VIL39_10875"
     CDS             323..733
                     /locus_tag="VIL39_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5481976.1"
                     /translation="MKAQRILVVEDDKVTQKVIADTLRSAGYEVATAHNGATAVKLAR
                     ELEPDLVTLDIQLAKDSPDDTWDGFGVANWLRRIAEGKQMPAIVVISGLDPEKTIESA
                     ASVGACTFLPKPFEKKRLLEVVAAALEQAPPTSP"
     gene            complement(723..2255)
                     /gene="trpE"
                     /locus_tag="VIL39_10880"
     CDS             complement(723..2255)
                     /gene="trpE"
                     /locus_tag="VIL39_10880"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5481977.1"
                     /translation="MYSPTLEEFLKLAARGNLIPVTRRLLADVETPLSAYRKIRGPGE
                     SFLFESVEGGEHLGRYSFVGCNPRAIIRQVGQTVEVIENGKVIETFGIQPETGTGSPA
                     SAASLGDGLAAVERVMKKYHAVPVPGLPRFTGGAIGFIGYEFIHDVEPVVPRPPHDDL
                     QTPVIYFLIADQLLIFDRVAQTITVLVNAVLEDAASPAEAYENATGEIDRLIALLEQP
                     TEHHPVTVCHEVPPLAFESNLPKEKFMANVLKAKQYITSGDIIQVVGSQRFSAPVKAS
                     PLDIYRAARSINPSPYMFLLELDGFSLVGASPEIHVRCEEGKVEIRPIAGTRRRGATP
                     AEDLALEKELLADPKERAEHVMLVDLARNDLGRVCDYGTVQVKDLMIIERYSHVMHIV
                     SEVEGRLSKDKTTYDLMRATFPAGTLSGAPKIRAMQIIAELEQTARGPYGGCVGYFSF
                     NGNLDCCITIRTALIKDGKAWVQSGGGWVNDSTPEGEYQETVNKSKAMLKAVAMAEGF
                     RD"
     gene            complement(2272..2913)
                     /locus_tag="VIL39_10885"
     CDS             complement(2272..2913)
                     /locus_tag="VIL39_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481978.1"
                     /translation="MEDFVPFASRSYALLFLLLFVSRGMDFLSTWVATPNMVLEGNPI
                     AKKLGWKWGILVNLALCIGLAFWPLPAIVISTTSVLVASRNFQSAWLMRSLGEQIYRD
                     WHVERVQETSVTLYLFCLFAQTALFGGVGAAVIGFSEWRLVPLAIGLGIVAYALAVAF
                     YTLLGIWRLRRSAILIARLAKRKLAVGNIQPAPGRNGKAMFNPAQPDTQPQGK"
     gene            complement(2985..3374)
                     /gene="hisI"
                     /locus_tag="VIL39_10890"
     CDS             complement(2985..3374)
                     /gene="hisI"
                     /locus_tag="VIL39_10890"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY5481979.1"
                     /translation="MSFYDKLKFDANGLIPAIIQEQKTGRVLMMAWMNRASLESTIKT
                     GKTHFWSRSRQKFWMKGESSGHTQTVKSIAFDCDGDTLLIQVDQIGAACHEGYQSCFF
                     RTVQGDGTTFKVTEEQLQTPGQIYGKK"
     gene            complement(3431..>3692)
                     /locus_tag="VIL39_10895"
     CDS             complement(3431..>3692)
                     /locus_tag="VIL39_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEY5481980.1"
                     /translation="FSNVPFVLVAGKWINNFAQPELMWKVMALTTTFAGNLTIVGSVA
                     NIIVVESARGHVELGFWDYARYGIPVTILTTVAGMLILMLFS"
BASE COUNT          727 a         1076 c         1181 g          708 t
ORIGIN      
        1 gcgcgcggct tctttgtccg cgcccgcaac ctcgatttcc agatacttgc cgacatgcac
       61 ggcgccgacc ccggtgtaac ccatctgagc cagggcgttc tgcacggtct tgccctgcgg
      121 atcaagcacc gctttctttg gcgtaatgac aattttcgct ttcatggtcg aatgggattc
      181 ccaactgctg gtccgaccga atattggaga agcaagcggc caaaggccag aagtttctta
      241 acctgccgat tgccgcggcg ggcttgactc caagcgcctc ctggcggcaa actggaactc
      301 cagtggcaaa tgcaccgagc gtatgaaagc ccaaagaata cttgtagttg aagatgacaa
      361 ggtcactcag aaggtgatcg cagacactct caggtcggcg ggctacgagg tggcaaccgc
      421 ccacaacgga gctaccgccg tgaaactcgc ccgggagctg gagccggacc tggtgaccct
      481 cgacatccaa ctggccaaag attcgcccga cgatacctgg gacggattcg gcgtggccaa
      541 ttggctgagg cggatagccg aaggcaaaca aatgcccgcc atcgtcgtga tttccgggct
      601 ggacccggag aagaccatcg agagcgccgc ctccgtgggt gcctgcacgt tcctgcccaa
      661 accgttcgag aaaaagagac tattggaagt agtagctgct gctttggagc aagctccgcc
      721 gactagtccc tgaagccctc cgccatagcg acggccttca gcatggcctt ggacttgttc
      781 accgtctcct gatattcgcc ttcgggcgtt gaatcgttca cccagccgcc gccagactgc
      841 acccaagcct tgccatcctt gatcagcgcg gtgcggatgg tgatgcagca atcgaggttg
      901 ccgttgaagg agaaatagcc gacgcagccg ccgtaggggc cgcgcgcggt ctgctccagc
      961 tcggcaatga tttgcatggc gcggattttg ggcgcgccgc tgagcgtgcc ggcggggaag
     1021 gtggcgcgca tcaaatcata cgtggtctta tccttggaga ggcgcccttc gacctcagag
     1081 acgatgtgca tgacgtggct gtagcgctcg atgatcatga ggtctttcac ctgcacggtg
     1141 ccataatcgc agacgcggcc caggtcattg cgcgccagat caaccagcat gacgtgctcg
     1201 gcgcgctcct tgggatcggc gagcaattcc ttttccaggg cgaggtcctc cgcgggcgtt
     1261 gcgccgcggc ggcgggtgcc ggcgatgggg cgaatctcga ccttgccttc ctcacagcgg
     1321 acatggattt cgggcgaggc gccaaccagc gagaagccgt ccagttcgag caggaacatg
     1381 taaggggacg ggttgatgga gcgggccgcg cggtaaatgt cgagcggcga ggctttgacg
     1441 ggggcggaga agcgctggga gccgacgacc tggataatgt cgcccgacgt gatgtattgc
     1501 ttggccttga ggacgttcgc catgaacttc tccttgggca ggttcgactc gaaggctaat
     1561 ggcggcactt catggcagac ggttaccgga tggtgctcgg tcggctgctc caggagggcg
     1621 atgaggcggt cgatttcgcc ggtggcgttt tcatacgcct cggccgggct ggcggcgtct
     1681 tcaagcacgg cgttgaccag gacggtaatg gtctgggcga cgcgatcaaa gatgagcagt
     1741 tggtcggcga tgaggaaata gatcaccggg gtctgcaaat cgtcatgcgg cgggcgcggc
     1801 accaccggct cgacgtcatg gatgaattcg tagccgatga aaccgatcgc cccgcccgtg
     1861 aaccgcggca agccgggcac gggaaccgcg tggtacttct tcatcacgcg ctcgaccgcc
     1921 gccaagccat cgcccaagct ggctgcggag gcaggactgc ccgtgccggt ttcgggttgg
     1981 atgccgaagg tctcgattac cttgccgttc tcaatcacct caaccgtctg gcccacttgc
     2041 cggataatcg cgcgtggatt gcagcccacg aaggagtagc ggccgaggtg ttcgccgcct
     2101 tcgacggatt cgaacaggaa cgactcgccc ggtccgcgga tcttgcgata ggccgaaagc
     2161 ggggtttcca catcagccag cagccggcga gtgaccggga tcaggttgcc ccgggcggcc
     2221 agcttgagga actcttcgag ggtgggtgaa tacatgttgg ttgggcccgg gttattttcc
     2281 ctgcggttgg gtgtcgggct gcgcgggatt gaacatcgct ttcccgttcc tgccgggtgc
     2341 gggttggatg ttgccgactg ccagtttgcg tttcgccagg cgggcgataa ggatcgccga
     2401 acggcgcagg cgccagatac ccagcagagt gtagaaggcc accgccagcg cgtaggcgac
     2461 gatgcccagc ccgatcgcca gcggcacgag ccgccactcg ctgaaaccga tcaccgccgc
     2521 gcccactccg ccgaagagtg cggtttgcgc aaagagacag aacaggtaga gcgtgacgct
     2581 ggtttcctgg acgcgctcga cgtgccaatc ccggtagatc tgctcgccca gcgaccgcat
     2641 cagccaagcg gattgaaagt tgcgggaagc gacaagcaca ctcgtggtgc tgatgacaat
     2701 ggcgggcaac ggccagaagg cgaggccgat acagagcgcc agattcacca gaatgcccca
     2761 tttccagccg agcttcttgg cgatggggtt gccctccagc accatgttgg gcgtggccac
     2821 ccacgtgctg aggaaatcca tgccgcgcga cacgaacagc agcaggaaga gcagggcgta
     2881 gctgcggctg gcaaatggaa cgaagtcttc cattgcggct taccggcagc gccggcgttc
     2941 ctttcgtccg gtgtcaactc cggcctttgg aaagcaatca ggcattactt cttcccgtag
     3001 atctgcccgg gcgtctggag ttgttcttcg gtgaccttga acgtcgtgcc gtcaccctgg
     3061 accgtgcgga agaagcagga ctggtagcct tcgtggcagg cggcgccgat ctggtccacc
     3121 tggatcagaa gagtgtcgcc gtcgcagtcg aacgcgatac tcttgacggt ttgggtgtgg
     3181 ccgctggatt cgcctttcat ccagaacttc tggcgcgacc ggctccagaa atgggtcttg
     3241 ccggtcttaa tggtgctttc gagcgaggcg cggttcatcc aggccatcat cagcacccgg
     3301 ccggtcttct gttcctggat gatggccggg atcaggccat tggcatcgaa cttaagtttg
     3361 tcgtaaaagc tcatagtcgc gaatctacag tgacactatg atagcaaagt agcaaatccc
     3421 agcctgccga ctatgaaaaa agcatcagaa taagcattcc ggcgaccgtc gtcaggatcg
     3481 tgacggggat gccatagcgg gcatagtccc agaagcccag ctccacgtgg ccgcgagcgg
     3541 actccacgac gatgatgttg gcgacggagc ccacgatcgt gaggttgccg gcgaaggtag
     3601 tggtgagggc catgactttc cacatcagct cgggctgcgc gaagttgttg atccacttcc
     3661 cggctaccag gacgaagggc acgttggaga ac
//
LOCUS       DATCGS010000531         3725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_589991, whole genome shotgun sequence.
ACCESSION   DATCGS010000531 DATCGS010000000
VERSION     DATCGS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589991"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VIL39_10900"
     CDS             complement(<1..395)
                     /locus_tag="VIL39_10900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373581.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5481981.1"
                     /translation="MPASPNESSHLLSVLIFSPLLAAAVAAFIRSERLLRWWTLAFTT
                     AAALFSLQLYWRFDPETAAFQFRDFAHWIPALGINYAVGIDGISLLLVLLTTLIMPLC
                     VMASWRYIQTRVREFMICLLIMETAMVGV"
     gene            complement(576..2387)
                     /locus_tag="VIL39_10905"
     CDS             complement(576..2387)
                     /locus_tag="VIL39_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HEY5481982.1"
                     /translation="MNSFWLHPSLVLILGALVLPLVPARLKKGYLLLIPVLLFWRILA
                     MARGEFGQVHFLDWTLVFGRVDALSSVFGYIMSLMCILGTLYGLHVKDDAQHIASWFY
                     VAGSVGCIYAGDFITLFLFWELMAFSSVFLIWFRGRRESLFAGYRYLLVHVAGGVVLL
                     AGIVLHCKTPGNGWAFTLLDVHHPTTAIYLIMAGFILNAAVPPLHAWLPDAYGEATFN
                     GSVFMCAFTTKTAVYALCRGFAGMEILVVLGVIMALYGVVYAVLENDCRRLLAYHIIS
                     QVGYMVAAVGLGTQMAINGACAHAFAHILYKGLLFMGCGAVLHMTGQSKFTELGGLWK
                     KMPWTFVFTLIGGLSISAFPLFSGFVSKSMIITAGLEEHKLWVGFLLLLASAGTFLHT
                     GLKVPYFIWFGKNRPKKETWDRAAEPPWNMNAAMLVASALCIFIGCYTPYLYKMLPYP
                     ELASEYHPYTSYHIFETLQILLFTGLGFFLLLKKLVPTPTISLDMDWFYRKGGRVFLW
                     FARKPVQLVDTWIGELYRVAGLVPLMISARVSGLFDNEVVDGAVDGLANTVRGIGRRL
                     RFAQRGQMQQSLAFAFAVAAALIIAFLLYSNFNALAK"
     gene            2621..>3725
                     /gene="trpB"
                     /locus_tag="VIL39_10910"
     CDS             2621..>3725
                     /gene="trpB"
                     /locus_tag="VIL39_10910"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5481983.1"
                     /translation="MSSIATNNVPDARGHFGPYGGRYVPETLMHPLQELEAEYFRAQQ
                     DAEFQREFDYYLREFCGRPTPLYFAERLTRELGGAQIYLKREDLLHTGAHKINNCIGQ
                     ILLARRMGKTRIIAETGAGQHGVATATVAAMFGLKCVVYMGAVDCERQSLNVLRMKML
                     GAEVVSVKAGQQTLKEAINEAMRDWVTNIRTTHYILGTVYGAHPYPLMVRNFQRVIGD
                     EARRQILEKKKRLPDFLVACVGGGSNAMGLFYPFLNDESVKMVGVEAGGEGIAAGKHA
                     ARFQGGSLGVLQGARSYLLQDEFGQVQLTHSVSAGLDYAAVGPEHAWLRDLKRVEYTY
                     ATDDQALKAFTELARLEGIIPALESAHAVAEVIR"
BASE COUNT          798 a         1130 c         1188 g          609 t
ORIGIN      
        1 aacacgccca ccatagccgt ttccatgatc agcaggcaga tcatgaactc cctgacccgc
       61 gtttggatgt agcgccagga ggccatgaca cacaacggca tgatcagggt cgtcagcagc
      121 accagcagca ggctgatgcc atcaatgccc acggcgtaat tgatgcctaa ggctggaatc
      181 caatgggcaa agtcgcggaa ctggaaggcc gctgtctcgg ggtcgaaccg ccaatagagc
      241 tgcagggaga acagggcggc ggcagtagtg aacgccagcg tccaccagcg caagaggcgc
      301 tcgctacgga tgaaggccgc gaccgcggcg gccagcagcg ggctgaagat cagcacgctc
      361 agtaggtggg agctttcgtt tgggctagcc ggcataatta acagcgcgaa attccagtgt
      421 cctgccgcgc gaagcgtttg gagtgcgacc agcttgctgg cgctttggac cggcgccgcg
      481 cgtccgaaag cgggagcaat ctccacgcac tccaaacgct tcgcgcaatt cggtggcggc
      541 tttgccgcgc tgcgaaatat ccgggctagg agtactcatt tggccaaggc gttgaagttg
      601 gaatacaaga gaaaggcgat gatgagcgcc gccgcgaccg cgaaggcgaa agcgaggctc
      661 tgctgcatct ggccacgttg ggcgaagcgg agccgccgcc cgatgccgcg gacagtgtta
      721 gccaggccgt ccaccgcgcc gtcaaccacc tcgttatcaa acaggcccga tacgcgggcg
      781 ctgatcatga gcgggacgag cccggccacg cggtagagtt ccccgatcca ggtatccacg
      841 agctgaaccg gcttgcgggc gaaccagagg aagacgcgcc cgcctttgcg gtagaaccag
      901 tccatatcca ggctgatggt gggggtcggt accagtttct tgagcagcag gaagaagccc
      961 aggccggtga agagcagaat ctgcagtgtc tcgaagatgt ggtaggaggt gtagggatga
     1021 tattcgctcg ccagctcggg atacggcagc atcttgtaga ggtaaggcgt gtagcagccg
     1081 atgaagatac acagtgcgga ggcaacgagc atggccgcgt tcatgttcca cggcggctcg
     1141 gcggcacgat cccaggtttc cttcttgggc cggttctttc cgaaccagat gaagtaaggc
     1201 accttgagcc cggtgtgcag gaacgtgcct gcggaggcca gcagcagcag gaagccgacc
     1261 catagtttat gctcctcaag gcccgcagtg atgatcatgg acttgctcac aaacccgctg
     1321 aagagcggga aggcggagat ggacaagccg ccaatgaggg tgaagacaaa cgtccaaggc
     1381 atcttcttcc atagcccgcc cagctccgtg aacttgctct ggccggtcat gtgcagcacc
     1441 gcgccgcagc ccatgaacag gaggcccttg taaaggatgt gggcgaacgc atgggcgcat
     1501 gcgccgttga tggccatctg cgtgcccaac ccgacggccg ccaccatgta gccgacctgg
     1561 ctgatgatgt ggtaggcgag caggcggcgg cagtcgttct ccagcacggc ataaacgacg
     1621 ccgtagagag ccatgatgac gccgagcacg acgaggatct ccatgcccgc gaagccgcgg
     1681 cagagggcat agacagccgt cttggtggtg aaggcgcaca tgaacaccga tccattgaac
     1741 gtggcctcgc cgtaagcgtc cggcagccag gcgtgcaggg gcggcacggc ggcgttgagg
     1801 atgaaacccg ccatgatcag gtaaatggcg gtggtcgggt ggtggacatc caacaaggta
     1861 aaagcccagc cgttgcccgg cgtcttgcaa tgcagcacga tgccagcaag caagaccacg
     1921 cccccggcga cgtgcacgag caggtaacgg tatcccgcga aaagggattc acgccggccc
     1981 cggaaccaga tgaggaacac ggaagagaac gccatcagct cccagaagag gaacagggtg
     2041 atgaagtcgc cggcgtaaat gcagccgacg gacccggcga cgtagaacca cgaagcgatg
     2101 tgttgcgcat cgtccttcac gtgcaagccg taaagcgtcc cgaggatgca catcaggctc
     2161 atgatgtagc cgaagaccga gctcagagca tccacccggc caaagaccag cgtccagtcg
     2221 aggaaatgga cctggccgaa ttcgccgcga gccatggcca gtatgcgcca aaagagcagg
     2281 actgggatga gcagcaggta tcccttcttc aagcgcgcgg gcaccagggg cagcaccagg
     2341 gctcccagga tcaggacgag cgagggatgg agccagaagc tattcatggt gtcccccctt
     2401 cggagtgcgc ccgtccccgg gcgcagcagg cttcgacttg cctggcgtgc tggaatgctc
     2461 cgagccccta ttgcctgcgg acgttgctgc gcccggggac gggcgcactc cggctccagt
     2521 cggtctgggc ggcgggggta tgtgcaagat gcgcccgtcg tgggaagccg gtcaaccatg
     2581 cgctcgacga gcagtgccct gaggggctat cctgcggagt atgagctcga ttgccactaa
     2641 taatgtgcct gatgcgcgcg gccacttcgg tccgtatggc ggccggtacg tgcccgaaac
     2701 gctaatgcac ccgctgcagg aattggaggc agagtatttt cgcgcgcaac aggacgctga
     2761 gttccagcgc gagttcgact actacctgag agaattctgc ggccggccca ctcctcttta
     2821 cttcgccgaa cgcctcaccc gggaactcgg cggggcgcag atctacctga aacgggaaga
     2881 cctgctccac accggcgccc acaagatcaa caactgcatc ggccaaatcc tgctcgcccg
     2941 gcgcatgggc aagacgcgca tcattgccga aaccggcgca ggccagcacg gggtcgcgac
     3001 ggcgactgtc gcggctatgt tcgggctcaa atgcgtcgtc tatatgggcg cggtcgattg
     3061 tgagcggcag tcgctcaacg tcttgcgtat gaagatgctc ggcgcggaag tcgtttcggt
     3121 taaagccggc cagcaaacct tgaaggaggc tatcaacgag gccatgcgcg attgggtcac
     3181 caacatccgc actacccact acatcctcgg cacggtctat ggcgcccatc cctacccgct
     3241 gatggttcgc aacttccagc gcgtcatcgg cgacgaagcc cgccggcaga ttctcgaaaa
     3301 gaagaagcgc ctgcccgact tccttgtcgc ctgtgtgggc ggcggttcca acgccatggg
     3361 cctgttctat cccttcctca atgacgaatc ggtgaaaatg gttggcgtcg aagcgggggg
     3421 cgaaggcatc gccgccggaa aacacgccgc ccggtttcaa ggcggctccc tcggcgtctt
     3481 gcagggcgcg cgctcttacc tattgcagga tgagttcggc caagtgcagc tcacccacag
     3541 cgtctcagcg ggtctggact acgccgccgt cggccccgaa cacgcctggc tgcgcgacct
     3601 caagcgcgtc gaatacacct acgccaccga cgaccaggcg ctcaaggcgt ttaccgagct
     3661 cgcgcgcctc gaaggtatta tcccggccct ggaatccgcc catgccgtcg cggaagtcat
     3721 ccgtc
//
LOCUS       DATCGS010000532         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_639218, whole genome shotgun sequence.
ACCESSION   DATCGS010000532 DATCGS010000000
VERSION     DATCGS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639218"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VIL39_10915"
     CDS             <1..1142
                     /locus_tag="VIL39_10915"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HEY5481984.1"
                     /translation="TSSHRPTAVITHHASRIPHHASRRSQQGIALVIVMISIMVLTIL
                     AAGFAYSMKVETKLARNANSEAELEWLGRSGVEYARWVLANSLLDPTQPYDSHDQPWF
                     TGSGWLGPTNAPIAEVQKTFTLGRGSVTWTITDLESKFNINSPEPILQQVLPKAFELM
                     GVAPGESTPLVNAILDWIDPDDQTHIQGAESEYYQNLTPPYSAKNGPIDDISELLLIK
                     GMMPEIYYGLAATNYQPSYYSQQRGRLAQQAAMLAAPVGLTDLFTPLSDGKVNINTAS
                     AEVLQLIPGVDPMIAEAIISGRAGEADLSAPGMLGPYRSVNEVSRMPNVPPEIVRQLS
                     QFCDVRSKTFQVQVDADVGGYKRTFFAVLGRNNPRDVQILSFYWK"
     gene            complement(1221..2267)
                     /locus_tag="VIL39_10920"
     CDS             complement(1221..2267)
                     /locus_tag="VIL39_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481985.1"
                     /translation="MKRTAASVLTILAFLQLGIHLALGWDYEAHRAVNQLGLASLPTN
                     FPAFVRQPAAAERIAFLAGEMDRWRNTQDLSLKQYSFPDHYMDMEELADYGLKAELLP
                     IFRYDFIAQLALIRKAHPDKFPEPDAASNQDHTRGLLGLLPWGIAESVGKLKSGFSYL
                     KAFEEGGTPEEIANAQANIIYIMGVMGHLVGDASQPLHTTIHHHGWVGDNPHQYTTSR
                     GFHSWIDGGYLNKTGGANVEALKSRLHPAQPVSISGRPAKPEEMFQAAMLFLIEQNKQ
                     VEPLYQLDKDGKLSGEGEKGLEGKAFLESQLLKSGQLLGDFWYSAWEQAPPDSFLKGQ
                     LAKRKRAASAAENK"
     gene            complement(2551..3783)
                     /locus_tag="VIL39_10925"
     CDS             complement(2551..3783)
                     /locus_tag="VIL39_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY5481986.1"
                     /translation="MTSRERVLAALNHQVPDRVPIDLSGHRSSGIAAMAYARLRDYLG
                     LPKKPIRVYDPVQQLAIVDEDVLQRFQVDTIELGRAFALEDKYWAEWTLPDGTPCLMP
                     AWAQPTRDNGEWVVRSQVTGRVIARMPENVWYFEQTNFPFLEQDDPDQIEAAIPECMW
                     TAMASPPGPIAAGPDGARVLQDGARRLRQQTDRAIIGLFGGNLLEFGQFLYRNDNFFM
                     VLAGEPERAHRFLDKLLEMHLRNLDGFLSAVGDYIDVILFGDDLGMQSGPQVSPAMYR
                     EYFQPRERAMWQLVKQRAPHLKIMLHCCGGVRELLPGLIDAGLDAINPVQITCRGMEP
                     AGLKRDFGNRLTFWGGGCDTREVLIKGTPAQVREHVRQQVKIWRPGGGFVFQQVHNIL
                     ADVPPANIVAMYEAAREA"
     gene            complement(3971..4213)
                     /locus_tag="VIL39_10930"
     CDS             complement(3971..4213)
                     /locus_tag="VIL39_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY5481987.1"
                     /translation="MKVPRDLSGAQLIKALCRDWGYRQVHQEGSHVILQTETPGHQRL
                     SVPNHNPLRVGTLNGIVRAVATHKSADRQTVLDSLR"
     gene            complement(4210..4422)
                     /locus_tag="VIL39_10935"
     CDS             complement(4210..4422)
                     /locus_tag="VIL39_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY5481988.1"
                     /translation="MSELVFEVTQEEDGGFCAECLSENIFTQGDTWEAVRQNVQEAVR
                     AYYFDQPESPQRIRLHLVRDELLAGA"
BASE COUNT          869 a         1464 c         1342 g          873 t
ORIGIN      
        1 ccacctcatc ccatcgcccg accgccgtga tcacgcatca cgcatcccgc atcccgcatc
       61 acgcatcacg ccgctctcag caagggatcg ccctggtcat cgttatgatc tcgatcatgg
      121 tcctgacgat tctggccgcg gggtttgcct attccatgaa ggtggagacc aagctggccc
      181 gcaatgcgaa cagcgaagcg gagctggaat ggctgggccg ctccggtgtc gagtatgcgc
      241 gctgggtcct agcgaattcg ctgttagacc ccacgcagcc ctacgattcc catgaccagc
      301 cctggttcac cggatcgggc tggctcggcc ccaccaacgc ccccatcgcc gaggtgcaga
      361 aaaccttcac gctcggacgt ggcagtgtta cctggaccat caccgacctc gaaagcaagt
      421 tcaacatcaa ctctcccgag cccatcctgc aacaggttct gccgaaagcc ttcgagctga
      481 tgggcgtcgc cccgggcgag tcaacccccc tcgtcaatgc catccttgac tggattgacc
      541 ccgatgacca gacccacatc cagggtgccg agtccgaata ttaccagaac ctcactcctc
      601 cttattccgc caagaacggc ccgattgacg acatctccga gctgcttctg atcaagggca
      661 tgatgccgga aatctactat ggcctcgcgg cgaccaatta tcagccgagt tattacagcc
      721 agcaacgtgg ccggcttgcc cagcaggccg ccatgctggc cgcgcccgtc ggtctgaccg
      781 acctttttac gccgctgtct gacggcaagg tcaacatcaa caccgcctcg gcggaagtcc
      841 tccaactcat cccgggcgtt gatcccatga tcgccgaggc gatcatcagt ggccgtgctg
      901 gcgaggcgga cctcagcgct cccggcatgc ttgggccgta ccggagtgtg aatgaagtaa
      961 gccgcatgcc caacgtcccc cccgaaatag tgcggcagct ctcccaattc tgcgacgtgc
     1021 gcagcaagac cttccaggtg caggtggatg cggatgtcgg cggctacaag cgcaccttct
     1081 ttgccgtcct gggccgcaac aacccgcgcg acgtgcaaat cctcagcttc tattggaaat
     1141 agcggttcat ccgggaagtc tgagtcggat agaacacggc ggaacgaagc gctggtctgc
     1201 gacccggcgt ggttcgcagg ctacttgttc tccgccgcgc tcgccgcccg cttgcgcttg
     1261 gcgagctggc ccttgagaaa gctgtcgggc ggggcctgtt cccaggcgga ataccagaag
     1321 tcgcccagca actggccgga cttgagcagt tggctttcaa ggaacgcttt gccttccaat
     1381 cccttctcac cttcgccgga aagcttgccg tccttgtcca actggtaaag gggctcaacc
     1441 tgcttgttct gctcgatgag gaagagcatg gccgcctgga acatttcctc gggcttggcg
     1501 gggcggccgc tgatggagac tggttgggcc ggatgcagcc gactcttcag cgcctccaca
     1561 ttggcgccgc cggtcttgtt gaggtatccg ccgtcaatcc aactgtggaa cccgcggctg
     1621 gtcgtgtact gatgcgggtt gtcccccacc cagccgtgat gatggatggt ggtgtgcagg
     1681 ggttgcgagg cgtcgccgac gagatggccc atcacgccca tgatgtaaat gatgttcgcc
     1741 tgggcattgg cgatttcctc cggtgtcccc ccctcttcaa atgccttgag gtaagaaaag
     1801 ccggatttca gcttgccgac cgattccgca atgccccagg gcagcagccc gaggagcccg
     1861 cgagtatggt cctggttgct ggcggcatcg ggctcgggga acttgtccgg gtgcgccttg
     1921 cggatgagag cgagttgggc aataaagtcg tagcggaaga tcggcagcag ctccgccttc
     1981 aggccgtaat cggccagctc ttccatgtcc atgtaatgat cggggaagct gtactgcttg
     2041 agggacaggt cctgggtatt gcgccagcgg tccatttcac cggccaggaa ggcaatgcgc
     2101 tcggccgccg ccggctggcg cacgaaggcc gggaaattgg tcggcagtga ggcgagccca
     2161 agctggttga cggcacggtg agcttcataa tcccacccca gagcgaggtg gatgccgagt
     2221 tgaaggaacg cgaggatcgt gaggacggaa gcggctgttc ttttcatgcg caatattcga
     2281 ttgtcccgca tactgtagtg tccccgccgg aaaaggcaaa tcctcccagc gattctcatt
     2341 atggtcctgg ccaagacccc gcacacccga cgcgcgcagg cgatcacgtg gaaagcctgg
     2401 gtcctgggga tgcttgcagg agcgttcctt tgagcagcgc accctgcgtt acgaggacgt
     2461 gcgcgagatg atgggggagg cgcggccaat gatcctcgaa aaggcccaag ccacgggcaa
     2521 gtcctgaatc cgtcaatcac gcatcacgca tcacgcctcc ctcgccgcct catacattgc
     2581 cacaatattg gccggcggca cgtccgccag gatgttgtgc acttgctgaa agacaaagcc
     2641 tccgccgggc cgccaaatct tcacctgctg gcgcacatgc tcacggactt gcgctggcgt
     2701 ccctttgatc agcacctcgc gcgtgtcgca accaccgccc cagaaggtga gccgattgcc
     2761 aaagtcacgc ttgaggccgg ccggctccat gccccggcag gtgatttgca ccgggttgat
     2821 ggcgtccaag ccagcgtcaa tcagaccggg cagcagctcg cggacgccgc cgcagcagtg
     2881 cagcatgatc ttcaagtgcg gcgcgcgctg cttgacgagc tgccacatcg cgcgctcgcg
     2941 cggctggaag tattcgcgat acatcgcagg cgaaacctgc gggccggatt gcatgcccag
     3001 gtcatcgccg aacaggatca catcaatgta atcccccacc gcgctcagga acccgtcgag
     3061 attgcgcagg tgcatctcca acagcttgtc caggaagcgg tgcgcgcgct ccggttcgcc
     3121 ggccaggacc atgaagaagt tatcgttgcg gtagaggaac tggccgaatt cgagcaggtt
     3181 gccgccgaag agcccgatga tcgcgcgatc cgtctgctgc cgtaggcggc gtgcgccatc
     3241 ctgaagcacc cgggcaccgt ccggtccagc ggcgattggc cccggcgggg acgccatcgc
     3301 cgtccacatg cattcgggaa tcgccgcctc gatctggtca ggatcgtcct gttccagaaa
     3361 cggaaagttc gtctgctcga aataccacac gttctcgggc atgcgagcaa tcacgcgccc
     3421 ggtgacctgc gagcgcacca cccactcgcc gttgtcccgg gtcggctggg cccaggccgg
     3481 catcaggcag ggcgtcccgt ccggcagcgt ccactccgcc cagtacttat cttcgagggc
     3541 aaaggcccgg cccagttcaa tcgtgtccac ctgaaaccgc tgcaacacgt cctcatccac
     3601 gatggcaagc tgctgcaccg ggtcatagac ccgaatgggt ttcttgggca ggccgaggta
     3661 atcgcgcagc cgcgcgtacg ccatggcagc gatgcccgac gagcggtggc cggataaatc
     3721 aatcggcacc cggtcaggta cctggtggtt cagcgccgcg aggactcgtt cacgtgaggt
     3781 cataactttg ccgtctggtg tttcaggtgt tcagccgacc gcgacagcct gcccgcgcat
     3841 ccggcagagg tcaaaggcaa aacgggcaaa acggacctga gcctgcggaa tcaccttgga
     3901 tcgagcgtat ccggtaaaac acgtctcgga ctattcgtcg cttggggtca gcagtcgaac
     3961 cacctggaag tcaccgcagc gagtccaaca cagtctggcg atccgcgctc ttgtgcgtcg
     4021 ctaccgctcg cacgataccg ttgagcgtgc cgacgcgcaa aggattgtga ttgggaacgg
     4081 acagtcgttg atgcccgggc gtctcggtct gcaaaatgac gtggctgcct tcctgatgca
     4141 cctggcgata tccccaatcg cggcacagcg ctttgatcaa ttgcgcgccg cttaaatccc
     4201 gtggcacttt catgcgccgg ccagcagttc gtctcgcacc agatgcagcc ggatgcgttg
     4261 gggagactca ggttggtcaa agtaataggc gcgcaccgct tcctggacgt tctggcgcac
     4321 cgcttcccaa gtatctccct gcgtaaagat gttctccgaa aggcattcgg cgcagaagcc
     4381 cccgtcttcc tcctgcgtca cttcaaaaac cagttcgctc atgcggctca agttacgggg
     4441 tttgactcgt tatcgcaagt ggcgggaagc cctggcccat cactccactt ttttccggtg
     4501 tcaggttctt cctgggtccc aatcccgccg tcgtcccgaa acccaccg
//
LOCUS       DATCGS010000533         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_491577, whole genome shotgun sequence.
ACCESSION   DATCGS010000533 DATCGS010000000
VERSION     DATCGS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491577"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..398
                     /locus_tag="VIL39_10940"
     CDS             108..398
                     /locus_tag="VIL39_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481989.1"
                     /translation="MPLLRQPLIRLLAINLAAGASVAVLMLGGLLALNPAGLRDLILA
                     DRAGGAALALLLFGFLITFSSAAMGTAIMALGARPESGRRGGKAQAVEARVR"
     gene            664..1482
                     /locus_tag="VIL39_10945"
     CDS             664..1482
                     /locus_tag="VIL39_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5481990.1"
                     /translation="MKNRIAGITSLTSGSILFAGLQLIALSALIVAATETRAAEIRVL
                     SGGAPKEALAVLTPEFEKQTGHKVRYTFGVVTVLRQKLDAEEKTDMVMMPVPIIDAYV
                     KSGKMRAEGRAALGGVGIYVIVRQGAAIPDISTPEAFRKALLDARSVVHATPTATPSG
                     AHMAKVIEQLGITDAMQKKVIHRPALDGGAELVAKGEADLGIYPTSEVSHVKGITLVG
                     PMPSALQLITVYGAAAMADNAAPEPALAFIKFLAEPANRKHWKDAGFDPVWSKN"
     gene            1605..2159
                     /locus_tag="VIL39_10950"
     CDS             1605..2159
                     /locus_tag="VIL39_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5481991.1"
                     /translation="MEWGRILAYITGTVDQELLLRNEYLAAENRILKAQLKGRLRLSD
                     AERAKLGEIGHRLGRKTLGEVANAALPDTILAWYRRLVARKFDGSRERRTPGRPRINR
                     EVEELIVRMAEENRSWGYDRIVGAWPIWGMRSRIKRSAMFCNATVYRPRRSASTRPPG
                     RHSFGLTWRGPTFSRWRSSRCEDW"
BASE COUNT          449 a          643 c          735 g          418 t
ORIGIN      
        1 gccggcgcgg tgggcttgtc ccggattgca ggatttcgcg gcctgaggac cgagaacgcc
       61 cgacgcgccg ctataaagaa cttatcgcaa ctcgaaggcc cacgcgcatg ccgcttctcc
      121 gccaaccgct catccggctt ctcgcaatca atctcgccgc cggcgcgagc gtggccgtgc
      181 tgatgctcgg cgggctgctg gcgctcaatc cggcgggctt gcgcgacctc atcctggccg
      241 accgcgcggg cggcgcggcg ctggcgctgc tgctgttcgg ctttctcatc accttcagca
      301 gcgcggccat gggcaccgcc atcatggcgc tgggcgcaag gcccgagagc ggcaggcgcg
      361 gcggcaaggc acaagcggtt gaggcgcggg tgcggtaggt gactctagac gttcgatggt
      421 catgcgttca agagcaaccg acgggaggag tgcgtccgaa tgccagggag aacggccaga
      481 tcagacccga acgccattcg ggatgcaagg aaagccggtg gctggtcgta cctcaaatct
      541 tcgcttccag ctagttcaga aaacagctac caatcacgcc agtttgggag gcatctgggg
      601 agtcccgggc ttaactgaat ctagaaaaca gggcgtttgg ctaaaatcgc ctggaggggc
      661 accatgaaaa atagaattgc tgggatcacc agcttgacca gcggatcgat cctatttgcc
      721 ggactgcagt taatcgcgct ttcggcgctg attgtcgcgg cgaccgaaac ccgcgctgct
      781 gaaattcgcg tgttgagcgg tggagcgcca aaagaggcgt tggccgttct cacgccggaa
      841 ttcgagaagc aaacgggcca taaggtgagg tatacgttcg gggttgtcac ggtgctgcgt
      901 cagaaactgg atgccgaaga aaagaccgac atggtcatga tgccggttcc catcattgac
      961 gcctatgtga aatcagggaa gatgcgtgcg gaaggccgcg ccgcgctcgg cggggttggc
     1021 atctacgtca tcgtgcgcca aggcgctgcg atcccggata tttcgacgcc ggaagccttt
     1081 cgcaaggcgc tacttgatgc ccgttccgtc gtgcatgcta ctcctaccgc aacgccgagt
     1141 ggcgctcaca tggccaaggt gatcgagcaa ctcggcataa ctgatgcgat gcagaaaaag
     1201 gtaattcacc ggccggccct cgacggcggt gccgaactgg tcgccaaggg ggaggctgac
     1261 cttggcattt atcccacgag cgaggtttct cacgtgaagg gcatcacgct ggtgggacca
     1321 atgccgtccg cgctgcagtt aatcactgtc tatggcgctg ccgcgatggc tgacaacgcc
     1381 gcgccggagc ctgcactggc cttcatcaaa ttcctggcag aacccgccaa ccgaaagcac
     1441 tggaaagacg caggctttga tcccgtgtgg tcaaaaaatt aaaactgtag gaaaggcggg
     1501 cgttgtcccc tcctggcgtg gtctcgccgc gtgtcgtccg ctacgttttt ttggagcgat
     1561 caatggagcc ctatgcggat cggcgggcaa atggggggag acttatggaa tggggacgta
     1621 tcctggccta catcaccggg acggtggacc aggagctgct gctgcggaat gagtatctgg
     1681 ctgctgaaaa ccggattctg aaggcccagt tgaagggacg gctgaggctc tcggacgccg
     1741 aacgggcgaa gctgggcgag attggccatc gactgggccg caagaccctc ggcgaggtgg
     1801 cgaacgcggc cctgccggat accatcctgg catggtaccg aaggctcgtc gcccgtaagt
     1861 tcgatggctc gcgggaacgg cgaaccccgg gccggccacg gatcaaccga gaagttgagg
     1921 aattgattgt tcgcatggcc gaggagaatc ggtcctgggg ctatgatcgg attgtggggg
     1981 cctggccaat ctggggcatg aggtctcgga tcaaacggtc ggcaatgttc tgcaacgcca
     2041 cggtctaccg cccgcgccgg agcgcaagca cacgaccacc tgggcggcat tcattcgggc
     2101 tcacctggcg gggaccgact ttttcacggt ggaggtcctc acgctgcgag gactggtgac
     2161 ctactacgtg ctgtttttca tccatctcga gagccgcaag gtggacatcg ccgggatcac
     2221 cgttcacccg gatcagccgt ggatg
//
LOCUS       DATCGS010000534         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_43452, whole genome shotgun sequence.
ACCESSION   DATCGS010000534 DATCGS010000000
VERSION     DATCGS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43452"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VIL39_10955"
     CDS             <1..494
                     /locus_tag="VIL39_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5481992.1"
                     /translation="LDDHFTVYCKLSNGGKALVRASQICIGFKNDLGIVVAGTKGTLV
                     WRQEEAESLTILLPGQPDRVYWRGAVTPGDGFLPKDVPADLMAEPTSPSGHPEAFHDA
                     FARLHRSFEADVRKWKEGKAFKCDGAKYANVEDGWMGIAFIETCVKSSKKNGAWTPMP
                     KAI"
     gene            complement(725..1579)
                     /locus_tag="VIL39_10960"
     CDS             complement(725..1579)
                     /locus_tag="VIL39_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5481993.1"
                     /translation="MHRAISSTLTRREFLGAVGVAGAALGLGVTPLAQAADRKKIPVG
                     LQLYSLREQCKTDLPGMLTAVSKIGYRGVEFAGYHGRSAKELRKLLDDNSLVACGTHT
                     PYESILGDKLQETVEFNRTIGNKFLIVPWMNETKSKQEWLDRAKLFNDVAGKVKADGM
                     WVGYHAHQHDFKQIEGISAWDLFFGNTKPEVIMQLDTSNCCEGGADPVAVLKKYPGRA
                     RSIHLKAYGGGPDAVIGEDKVNWKEVFAFCEGKGKTQWYVVEHESAKDPLDAVKRSFE
                     GLRKFGKV"
     gene            1848..3716
                     /gene="rpoD"
                     /locus_tag="VIL39_10965"
     CDS             1848..3716
                     /gene="rpoD"
                     /locus_tag="VIL39_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5481994.1"
                     /translation="MARLKVNRTPPKPRARQQPQIPSESIDGFARRKSSAKSRALAAG
                     GSALARESRQGNASAARTASGPTAAAAASTRNPAPDQASLDITERIKELVRLAQEQGY
                     LTCSDITDALLDVQIGPEELDEIYAKLRNLEVEIVDQGEVDRIKQPGPDEEDEKARLD
                     ILDDPVRMYLKQMGQVPLLTREQEVEISKRIEAAENEVKRIICSLGFTAKEHIALAER
                     LTSEPPKERFDRAILDMKAAGREPHLRALRKLIPAVRAADQKVDEVYARWQACPSGPQ
                     RDRLRTRLRKLDRRLQDTFPKFFYKQKVIAEMALVAENIHDTIQSSLRLIQELEVQGK
                     LPSSQPLVESERRKIQALERFARAPWPDCLKACEQLKQASDQALQAKSEMVEANLRLV
                     ISIAKKYTNRGLSFLDLVQEGNMSLMRAVEKFEYRRGYKFSTYATWWIRQAVTRAIAD
                     QARTIRIPVHMIDAISKLLRLQKRLFQDFGREPTPGEIGDEMELPVERVRALLNMAQQ
                     PISLQSPVGENNETSFGDFIEDKATDSPAEKTSFSLLRDKISAVLASLTDREQKVLGL
                     RFGLSDGYSRTLEEVGRQFQVTRERIRQIEAKALRKMRHPTRIRQLHGFLEVQAPQ"
BASE COUNT          827 a         1225 c         1139 g          726 t
ORIGIN      
        1 aactcgacga ccacttcacc gtttattgca agctcagcaa cggcggcaaa gccctcgtgc
       61 gcgcctcgca gatttgcatc ggtttcaaga atgacctcgg catcgtagtc gccggcacca
      121 agggcacctt ggtttggcgc caggaagagg cggagtcgct cacgatcctg ctgccgggcc
      181 agcccgaccg cgtctattgg cgcggcgccg ttaccccggg cgatggtttc ctgcccaagg
      241 acgtgcccgc cgacttgatg gccgagccga cctccccgtc gggtcacccc gaggcattcc
      301 acgatgcctt cgcccgcctg catcgctcct tcgaagccga cgtgcgcaaa tggaaagagg
      361 ggaaagcctt caagtgcgac ggcgccaaat acgccaacgt cgaagatggc tggatgggca
      421 tcgccttcat cgaaacctgc gtcaaaagca gcaagaagaa cggcgcttgg accccgatgc
      481 cgaaggcgat ctaagccagt ccccatcccc caggcgagtg ttgttaaaac ccgcttggac
      541 gagtcttagg gcgttccgga acccagccgg aacgccctta attctttgta gcaagctggg
      601 attggctggc ttacgccgga ggccaagcgt gcccccgtga gtcacgaggt ttagcccgat
      661 cacatgatcc tgcgcgagcg tggtcagatt ggccttgaag acgtatcatg gccgcgggct
      721 gaggctacac tttgccgaac tttcttaggc cctcgaagct gcgcttcacg gcatcgagcg
      781 ggtccttggc actctcatgc tcgacaacat accactgggt cttgcccttc ccctcgcaga
      841 acgcgaagac ttccttccaa tttaccttat cctcgccgat gacggcgtcg ggtccgccgc
      901 cgtaagcctt tagatggata ctgcgcgcgc ggccgggata cttcttgagc accgccaccg
      961 ggtcggcccc gccctcgcag caattgctgg tgtcgagctg catgatcact tcgggcttgg
     1021 tgttgccgaa gaacaggtcc caggctgaga taccctcgat ttgcttgaag tcgtgctggt
     1081 gggcgtggta gccgacccac atgccgtcag ctttgacctt gccggcgacg tcgttaaaga
     1141 gcttggcccg atcaagccac tcctgctttg acttggtctc gttcatccac gggacgatga
     1201 ggaacttgtt cccgatcgtg cgattgaact cgactgtctc ttggagttta tcgcccagga
     1261 tcgactcgta aggcgtgtgc gtgccgcaag cgacgaggct gttgtcatcc agcagctttc
     1321 gcaattcctt ggcgctgcgg ccatggtagc ccgcgaactc cacgccgcgg taaccgatct
     1381 tcgacaccgc ggtcaacatg ccgggcaggt cggtcttgca ctgttcccgc agcgaataaa
     1441 gctgcaggcc aacgggtatc ttcttgcggt cggcggcctg ggccaggggt gtgacgccga
     1501 ggccaagggc agctccggcg acgccaacgg cgcccagaaa ctcgcgacgg gtgagggtgg
     1561 aggatatagc tcgatgcatg atatggtcct ttggtttgcg tattgttgaa tgaatgactg
     1621 acattgcgag tggaatctta agccggtcgg cccgcaggtg tcaagaaggt ccgaccgacc
     1681 aaataataga actttgggcg ctccacccgt ccttacccgg ccacaagaaa cagatgcttc
     1741 tgcggcgagt tgtgcgccgc accgatgtca gcttgactgc gttgcccgag acttccatat
     1801 tccaggctag caacttccgc tgctagatga tttgattcaa aagcactatg gccagattga
     1861 aggtgaacag gactccgccg aaacctcggg ccagacagca accccaaatc ccttcggagt
     1921 ccattgacgg ctttgcccgc cggaagtcgt ccgctaagag ccgcgccctt gccgcgggag
     1981 gttcagccct ggcgcgcgaa agccgccagg gcaatgcgtc agcggcccga accgcttctg
     2041 ggccaaccgc cgccgccgct gcctccaccc ggaatccggc cccagaccag gcaagtcttg
     2101 atatcaccga aaggatcaag gaactggtcc gcctggccca ggagcaaggt tacctcactt
     2161 gcagcgatat caccgacgcg ttactagacg tccagattgg gccggaggaa ctcgatgaga
     2221 tctacgccaa actgcgtaat ctggaagtcg agatcgtgga ccagggtgaa gtggaccgga
     2281 tcaaacagcc cggtccggac gaggaggacg agaaagcgcg gctcgacata ctggacgacc
     2341 cagtccgcat gtatcttaag caaatgggcc aggtcccgtt gctgacccgc gagcaggagg
     2401 tggagatttc caagcgcatc gaagccgccg aaaacgaagt caaacgcatc atctgcagtc
     2461 tcggtttcac cgccaaagag cacattgcgc tggccgaaag gctgacttcc gagccgccca
     2521 aagagcggtt cgaccgcgcc atcctcgaca tgaaagccgc cggtcgcgag ccgcacctcc
     2581 gagcgctgcg caaactgatc ccagccgtcc gcgcggcgga tcagaaagtg gatgaggttt
     2641 acgcgcggtg gcaagcctgc ccatccggcc cccaacgcga ccggctccgg accaggttgc
     2701 ggaagctcga ccgccgcctc caggatacct tccccaagtt cttctacaaa caaaaggtga
     2761 tcgcagagat ggcattggta gcagagaaca tccacgatac catccaatcc agcctgcgtc
     2821 tcatccagga gctggaagtt cagggcaaat tgccatcgtc ccagcccctg gttgagtcgg
     2881 agcggcgcaa gattcaggcg ttggagcggt tcgcgcgtgc gccgtggccc gactgcctca
     2941 aggcttgcga gcaattgaaa caggcctccg accaggcgct ccaggccaag agtgaaatgg
     3001 tagaagccaa cttgcgcctg gtcatctcca tcgccaagaa atacaccaat cgcggccttt
     3061 ccttccttga cctggtccag gagggcaaca tgagtttgat gagggcggtc gaaaagttcg
     3121 agtaccgccg aggctacaag ttctcgactt acgccacctg gtggattcgc caggccgtta
     3181 cccgcgccat tgctgaccag gcccgcacta tccgcatccc cgtgcacatg atcgacgcca
     3241 tcagcaagct cttgcgcctg caaaaacgtc tcttccagga tttcggtcgc gagccaacgc
     3301 cgggagagat cggtgatgaa atggaactcc cagtcgaacg ggttcgtgcc ctattgaata
     3361 tggcgcagca gccgatctcg ctccagtcgc ccgtcggcga gaacaacgag accagcttcg
     3421 gcgatttcat tgaagacaaa gccacggaca gcccggctga gaagaccagc ttcagcctac
     3481 tgagagacaa aataagtgcc gtgcttgcga gcttgaccga ccgggagcag aaagtcctgg
     3541 gattgcgctt cggtctgagc gatggctatt cgcgcacgct cgaggaagtc ggccggcagt
     3601 tccaggtcac ccgggaacgc attcgccaga tcgaagcgaa ggccctgcgc aaaatgcgac
     3661 accccactcg catccgccaa cttcacggtt tcctcgaagt gcaggcgccg caataggccc
     3721 ccccgggagc gcgagcgcca gtcgctgcaa tgctgacgaa cgccagccag ggcttacgaa
     3781 ggagctccat cagcggcgct ttgcgacagc ctcatcttgc tcaacagtct ttcggtgccg
     3841 cgtagcactt ctgtccggcg gggccctccc gcgttgctca ccagcggcac aaaggatcca
     3901 aagggcgcga aaaaggg
//
LOCUS       DATCGS010000535         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_145001, whole genome shotgun sequence.
ACCESSION   DATCGS010000535 DATCGS010000000
VERSION     DATCGS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145001"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VIL39_10970"
     CDS             complement(<1..1203)
                     /locus_tag="VIL39_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481995.1"
                     /translation="MTFWTLIRRSLRFHARAHLGVVLGAAIGSAALIGALVVGDSVRG
                     SLRQRALDRIGKVQYLLESRDRLFTDSTNTWRGNYAQAETLGLHLPSIASARQGAARA
                     NQVNLYGVQPGFWGFSEAGTNIHIETNGVFLNQPLAAQLRVSAGDTVLFRFRKPALFS
                     GEEPLSPQSRNSGAFQLKVQGILTAAQLGDFSPVGGMTPPRNAFMRLDQLQQAAGLNG
                     RANLVLEARITAYLPKLFSGVRLRLREWMAKLGFHPPNWLSPTALRDMLPEKGAKGLD
                     FLLGLSWSAADAGLDVEILEQQRVVELRSKRVFLDPPVVHGGTRTNTVWYQRSPARLP
                     TGRKELILTYLANLLQAGTNATPYSMITAAGPPYTPADMRDDDILVTDWLADDLHVQP
                     GDWIEMTSF"
     gene            complement(1396..2085)
                     /locus_tag="VIL39_10975"
     CDS             complement(1396..2085)
                     /locus_tag="VIL39_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5481996.1"
                     /translation="MKLVNVTRRYDSPANTGALTVLDQVSLEVARGESLAIVGPSGSG
                     KSTLLHIIGTLDRPTSGSVALDGQDLNGLGEVQIAAVRNRQIGFVFQAHYLLPQCTVW
                     ENVLVPTLACSDAALRDGAGERAERLLKRVGLGERKSHRPGELSGGERQRVAVVRALI
                     NQPQLLLADEPTGSLDHASAQALGQLLLDLNREEGVTLIVVTHARDLAKRMGRVFELK
                     DGRLAPIAIGP"
BASE COUNT          404 a          672 c          642 g          381 t
ORIGIN      
        1 gaagctggtc atctcaatcc agtcgcccgg ctgcacgtgc agatcatccg ccagccaatc
       61 agtgacaaga atgtcgtcat cgcgcatgtc ggcaggggtg tagggcgggc cagccgcagt
      121 gatcatggaa taaggcgtgg cattggtgcc agcctgcaga aggtttgcga ggtaagtgag
      181 aatcaactcc ttcctgccgg tggggagtct tgctggactg cgctgatacc aaacagtgtt
      241 ggtacgggtc cccccgtgca ccaccggggg atccaggaag acacgcttgg acctgagttc
      301 aaccacccgc tgttgctcca gaatctcaac atccagtccg gcatcggcgg cactccagct
      361 aagcccgaga aggaaatcca accctttggc gcccttttcc ggcaacatgt cacgtaaggc
      421 tgttggagaa agccagtttg gtggatgaaa tccaagcttc gccatccact ccctcagcct
      481 gagccggaca ccagaaaaca gtttgggcag gtaggctgtt attctcgcct cgaggacaag
      541 gtttgcgcgg ccattcaagc cagccgcctg ttgcagttga tccaaccgca tgaacgcgtt
      601 ccggggaggc gtcatgccac caaccgggct gaaatcgccg agctgtgctg cggtgagtat
      661 cccctgcacc ttgagctgga aggcccccga gtttctcgac tgcggtgata gtggttcctc
      721 gccggaaaac agggcgggct tgcgaaagcg aaaaaggacg gtatctccgg cactgacccg
      781 gagctgtgcg gcgagcggct ggttcagaaa cacgccgttc gtctcgatgt ggatattggt
      841 cccagcttcc gagaaccccc agaacccagg ttgtacgcca tacaagttca cctggttagc
      901 tcgcgcggca ccttgccggg cagaggcaat gcttggcaga tggagtccca gagtctcggc
      961 ttgtgcgtag ttgcccctcc aagtgttcgt agaatccgtg aagagccgat cgcggctctc
     1021 cagcaggtat tgcactttcc caatacggtc cagggcgcgc tgccgcaagc tccccctcac
     1081 cgaatcgccc accaccagcg ccccaatcaa ggccgcgctg ccgatggctg cgccgagcac
     1141 gacgcccagg tgcgcgcgcg catgaaagcg caggctgcgg cggatgaggg tccagaaggt
     1201 catagcctag gcgcaacacg aaacgcgagg cgcgcggaca ttcttgtccg cagcaacgcc
     1261 cgaactctcc ctggctccga acacgactgt ggcccacaaa ccgccccaca tcgctgcgga
     1321 caagaatgtc cgcgcgccgt cccgtttgca atttcgccgg gctgtgtaag gtgcgccacg
     1381 ctcttctcct tcctcttatg gcccgatggc gattggcgcg aggcgcccgt cctttagctc
     1441 gaacacgcgg cccatgcgct ttgccaggtc ccgggcatgc gtcacgacta taagggtcac
     1501 gccttcctcg cggttcaggt ccagcagcaa ttgcccaagc gcctgggccg aggcgtggtc
     1561 gagcgagccg gttggctcgt cggcgagcag gagctgcggc tggttgatca gcgcgcggac
     1621 cacggcgaca cgctgacgct ccccgccgga cagttcgccg ggacgatggg atttgcgttc
     1681 gcccagaccc acccgtttca ggagccgctc ggcccgttcg cccgcgccgt cgcgcagcgc
     1741 ggcatccgag cacgcgagcg tcgggaccag gacgttttcc caaaccgtgc attgtggcag
     1801 gagatagtgt gcctggaaga caaagccaat ctggcgattg cgcaccgcgg cgatctgcac
     1861 ctcgcccaga ccattcaaat cctggccatc gagcgccact gacccgcttg tcgggcgatc
     1921 gagcgtgccg atgatgtgca gcaacgtgct cttgcccgaa ccggaagggc ccacaatggc
     1981 gagcgattcc ccgcgcgcga cttcgaggga cacctggtcg agcaccgtca acgctccggt
     2041 gttggccggc gagtcatagc gcctggtaac gttgaccagt ttcagtagcg gcgctggcg
//
LOCUS       DATCGS010000536         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_145161, whole genome shotgun sequence.
ACCESSION   DATCGS010000536 DATCGS010000000
VERSION     DATCGS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145161"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VIL39_10980"
     CDS             complement(<1..464)
                     /locus_tag="VIL39_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481997.1"
                     /translation="MKRNRPINTSLLATALAAIGSLASAQAGSFFADFNDGLVPANAT
                     AYGNANVQGSGGYTNSGFLELTPAIGSQNAGFILNDLDAGVPVVSFTASYKCLIGGGG
                     YNGADGMSFNFASDLPAGTISQEGAGTGLTVEFDTFDNGLPDTAPSIDVMVGG"
     gene            complement(943..2370)
                     /locus_tag="VIL39_10985"
     CDS             complement(943..2370)
                     /locus_tag="VIL39_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5481998.1"
                     /translation="MLLAEVEVVSPDRPLPAGKTEAVLRFDDPTAREQAAKLLDDITG
                     HRVDGPQWGVIVAGALDQNHPAHLVVQADTVPSVTGLTAFLSHSHGPNNGGSTPGRNY
                     FQVERVGSNSFRLLLQSSGHVAADYLALSQPAVPDLDLLRKALERPCARMDGDYEQPF
                     ARPIPYFVRLRTVAQTLSQRAQCYLLLGKPEPAWHELALVRDMCRLLERKPTGNANTL
                     VEAMIDVAITGLYTAIVEDGLRLQVWREPELAEMQKQLKNINLLPLLRESMNAERAGV
                     CRMFEITPPAEFQELCFLGDGPRDLWGKLKNPRFLFVRFTPRGWVYQNMCALALRDQL
                     ISELFDVPNSQVLPCKADEIMNQLQTALGHFTPYTFLGARAVPNFVKAAQTLARNQTL
                     VNEAFIACGLERYRLVHGQYPETLEALVPQFAEQIPHDIIGAQPLKYHRTADGQFVLY
                     SVGWNEKDDGGVPGKTGMDGDWVWP"
BASE COUNT          475 a          736 c          769 g          479 t
ORIGIN      
        1 cctccgacca tcacgtcaat cgagggagcg gtgtccggca atccattgtc gaaggtgtcg
       61 aattcaacgg ttaggccggt gcccgcgcct tcctggctga tggtgccagc cggcagatcc
      121 gaggcaaaat tgaagctcat cccatcggcg ccgttgtagc cgccgccgcc gatcaaacat
      181 ttgtaggagg cggtgaagct gacgaccggc acgccggcgt ccaggtcgtt gaggatgaac
      241 ccggcgttct ggctgccaat agcgggcgtg agttccaaaa agccgctgtt ggtatatccg
      301 ccactgccct gcacgttggc attgccataa gcggtagcgt tggccggcac gaggccgtcg
      361 ttaaaatcag caaagaaact tcccgcttgc gccgaggcaa gcgagccgat agcggctaac
      421 gccgttgcaa gcagactggt attgattggt ctatttcttt tcatactaga ttttcttctt
      481 caatttgcgc tttcaaccgc ggtcggagcg accgcgggca gaacctggcg cttcgatcgg
      541 cccgggacga gaatgcaagt gaactcattg gctgaaggcg ataaagctac cacgagcgcc
      601 tgcccgccga agttccaatt gcatttaaca tatttcccgg acttcatgaa gtcgtccttt
      661 caatttgcgc gtctcgccga tgctcggcgt agtgatatac gataaactac ataaagttta
      721 gggcttctga gtgcgcaggc aagggaaatg tgcgggtccc tttcccggcg catccgggta
      781 ctgccctcag ttagcggcgg gcatctgaaa acaaaaagac cgctccgggg agcggccttc
      841 gcgaaactga gtgatgaggc ctatttgcgg cggcgcaacg ccagccaggt caacccgccg
      901 cacagcagca gagcggcctg ccgcgctcag tgctccggtg gcttacggcc agacccagtc
      961 gccatccatg cccgtcttgc cggggacgcc gccgtcgtcc ttttcgttcc agccgaccga
     1021 gtagagcacg aactgaccgt cagcggtgcg gtgatacttc aggggctggg ccccaatgat
     1081 atcgtgtggg atctgctcgg cgaactgcgg cacgagcgcc tcgagggttt cggggtattg
     1141 gccgtggacc aagcggtagc gttccagccc gcaagcgatg aacgcctcgt ttaccagggt
     1201 ttgattccgc gccaatgttt gggcagcctt cacgaagttg ggcaccgcgc gtgcgccgag
     1261 gaaggtgtag ggcgtgaagt ggccgagggc ggtttgaagt tggttcatga tttcgtcggc
     1321 cttgcagggc aggacctggc tgttggggac atcgaagagc tcgctgataa gctggtctcg
     1381 aagggcgagc gcacacatgt tttgatagac ccagccgcgc ggcgtaaacc tgacgaacag
     1441 gaatctcgga ttcttgagtt taccccacaa atcccgcggc ccatcgccga ggaaacacaa
     1501 ctcctggaat tcggcgggcg gggtgatttc aaacatgcgg cacacgccag cccgttcggc
     1561 gttcatcgac tcgcggagga gcggcagcag gttgatgttc ttgagctgct tctgcatctc
     1621 ggccagctcc ggctcacgcc aaacctgtag gcgcaaccca tcttcgacga tggccgtgta
     1681 aagcccggtg atcgccacat caatcatggc ttccactagc gtgttggcgt tgccggtcgg
     1741 cttacgttcc agcaggcggc acatgtcgcg gaccaatgcc agttcgtgcc acgcgggttc
     1801 cggcttgccg agcagcagat aacactgcgc ccgctgcgac agggtttggg ccacggttcg
     1861 gagcctcacg aagtacggaa tcggccgcgc aaagggctgc tcgtagtcgc catccatccg
     1921 ggcgcagggg cgctcgagcg ctttgcgcag caggtcgaga tcgggcaccg ccggctggct
     1981 cagggcgaga taatcggccg cgacatggcc tgaagactgc agcaacaggc gaaagctgtt
     2041 gcttccaacc cgctcaacct ggaagtagtt acggccaggc gtggacccac cattgttcgg
     2101 cccatggctg tgggacagga atgcggtgag ccccgtgaca ctcggcacgg tgtcggcctg
     2161 caccaccagg tgggccggat ggttctgatc caacgccccc gcgacgatca ccccccactg
     2221 agggccatcc acacggtggc cggtaatgtc gtcgagaagt ttcgccgcct gctcgcgggc
     2281 tgttgggtcg tcgaaccgca acaccgcctc agtcttcccc gcagggagcg gccgatcggg
     2341 ggacacgacc tccacctcgg ccagcagcac tgggtccttt ttggcatttc agcccggcgc
     2401 aagggggtag ggtgcattgg catacccagg cttggatggt ccaccggaga cagcctcag
//
LOCUS       DATCGS010000537         7091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_590373, whole genome shotgun sequence.
ACCESSION   DATCGS010000537 DATCGS010000000
VERSION     DATCGS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590373"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIL39_10990"
     CDS             complement(<1..227)
                     /locus_tag="VIL39_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY5481999.1"
                     /translation="MSLVTVFSAFNPMDAQLARSRLEAAQFHAVVTHELSALSMDGYA
                     LAAGGILVQVPAEEAAEAKEFLAADTPPDEP"
     gene            complement(261..1244)
                     /locus_tag="VIL39_10995"
     CDS             complement(261..1244)
                     /locus_tag="VIL39_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5482000.1"
                     /translation="MCTIGGYAVSQVRPEYVGSSYGWFLGMLIGFGFGGLMIALDEML
                     KGFSLRAFSATTFGLLLGTVVALLIDRSGLFENVEEHPTRWLIRLSLFLSFSYIGIVL
                     AMRSNKEDFSLIIPYVRFAPQNKPDNLLLLDTSVIIDGRIADLIEANFVEGLIVVPRF
                     VLKELQQIADSNDALKRARGRRGLEVLNRIQRNTRNEVKIHDGDFPEETEVDAKLVRL
                     ARNLGAKLYTNDHNLGQIAELQKVNHVNLHDLAKSLRVILLPGELLSLRIVREGKDKG
                     QGVGYLPDGTMVVVNNAQTAIGQQVEVQVQSQLQTGAGIIVFADLKQAVVA"
     gene            complement(1283..2278)
                     /locus_tag="VIL39_11000"
     CDS             complement(1283..2278)
                     /locus_tag="VIL39_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5482001.1"
                     /translation="MSKSRLEWKVGLFVFIGLALLVVLLLQFSKGLSFFRPTYDILLN
                     ASSVGGLKTRAQVLMSGVQVGTVSDMRLAPDGKSVTITLRLYKQPQIHKDARFVIEQS
                     GFLGDQYVAIRPTNNEGDFFGPGGEAKAEAPLDLQEVARFAAGFLQHIDSAATNLNDA
                     LMEARRTVLSARALTNLSATLNNLQRVSERSLIVVDNLNAIVATNGPAINVSVSNILF
                     FTEQINLAASAVRELVATNSPEIDAAVKNIESSTTTLKNLLDGVQQGKGLAGQLLENE
                     QIAANVSRVVENLSITTSNLNRLGLWGILRQHKPPSTNTPGPVLRPLTTPKNPFD"
     gene            complement(2280..3026)
                     /locus_tag="VIL39_11005"
     CDS             complement(2280..3026)
                     /locus_tag="VIL39_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5482002.1"
                     /translation="MIEVRDLKKSFGTHSILDGVSFRIEKGESVVIIGRSGGGKSVLL
                     KHLIGLLQPDAGQVLIEGENIVQMNERELLRVRHKFGMLFQSAALFDSMTVAENVGFA
                     FRRDRSLPKEEIREKVAEMLEMVDLPGTEDKKPSELSGGMKKRVGLARAVIYHPQIVL
                     YDEPTTGLDPIVSDSIDKLILRVRDRLDVTTVVVTHDMRSARRLGQRIMMLHNQRIYA
                     TGTPDEIFNSQDPVVRRFIEGVSDPKELYF"
     gene            complement(3023..3802)
                     /locus_tag="VIL39_11010"
     CDS             complement(3023..3802)
                     /locus_tag="VIL39_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5482003.1"
                     /translation="MNSWGKNWIADWLEGLGRVTLLAKESVASLLTFKVAWRDLLYQI
                     YFVGVKSQSVVLITGAFTGMVLAAQTYLQFHKVKMDTATLAVVSISMCSELGPVLTGL
                     MVAGQVGAAIAAELGTMRVTEQIDALRTLATHPVDYLVVPRLLALHIALPLLTAESIA
                     VGIGAGYLVGVRLYGIDPTYSWYNMLRYTSSSDVVVGLVKAVIFGGIVALISCYKGLS
                     CGEGAEGVGRATTEAVVYSSITILISNFFLTLTLGQILHAL"
     gene            complement(4346..>7091)
                     /locus_tag="VIL39_11015"
     CDS             complement(4346..>7091)
                     /locus_tag="VIL39_11015"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY5482004.1"
                     /translation="AWRSLGNHSHSRVETDPDNPAGHVLHLVATGSTETLHNHLETTY
                     ANGRSITNGKTYQISFRAKWLAGNNHLNTRLYFNRVAKTTVLPMPSQHGTPGGRNSTF
                     TAHLGPTFAAFGHSPIIPQPNQAVTVNVSASDPAGVRAVTLWWSVNGGVWQQAPMLSA
                     QVAASPGYTNYTGLISGQTAGALVQFYVQAADNLGVSTTFPARGRDSRALFKVDDGTA
                     LMPQLHRLRLLMTPADTALLLADTNVMSNDLIGLTVVHDERQVFYDVGVHLQSSERGR
                     DDPNRQGFTVKLHPEQPFRGFLETITMDRSGGYSGLGGTHGEILLWHAINHAGGLLGF
                     ECDLVQVFAPNPQLNGTAMLRISGFDGSYFDEQFQNNGNGNRYKLEVIYYPTTTLTGD
                     PQAPKLPQPDNVVNVDIQDWGNDPENYRWVFFQENNTDIDDYSQLMTFSKSFSLTGPA
                     LQTQTARLMDSDEWMRMLAFKAFTGDVDTFTAGYNHNFMVYFRQDDGKALGLLWDEDF
                     SFAAPVNAAFPGTSSPGMYNIVTLPDNYRRYYNHLLDLMTTTINSAHLSPWANRYSGL
                     LGQDWSGVVNYLQQRANFIRSAMPLTTAFAITNNGGRGFATTNNPIILGGIAPLTVKD
                     IRINAVSGAITWLSLTNWTVPVSLFNYSNFLAVQGFDNYGALVTNASVSIVVTNLGAA
                     ALRPVVFNEWMAKNNGPGGFADPVAGTFSDWFELYNPNSSAADISGFYFTDDLSNPTQ
                     SRVPAGTVIPPHGFLLVWADKNTELNGSGINGDLHVNFKLPQSGMTLGCFAANGTMQN
                     MVTFGAQLENVSQGLFPDGNTNAVYFFTDWSPRASNRLGPPPSPQLGVCVVQAGGTFA
                     FQASTIPGRTYRTEFKDQLDAPTWTTLGADLTATGSQIVVTDTFVGRPQRFYRVMLVP
                     "
BASE COUNT         1441 a         1989 c         2248 g         1413 t
ORIGIN      
        1 tcgggctcat caggaggggt gtcagcagca agaaactctt tggcctcagc ggcttcttcg
       61 gccggcacct gcacgaggat gccacccgcg gccagcgcgt agccgtccat gctcaacgcc
      121 gaaagctcat gggtcacgac ggcgtggaac tgcgcggctt ccagccggga ccgggccagt
      181 tgagcgtcca tggggttgaa tgcgctgaag acggtgacga gagacataag attgtaagct
      241 tttgcgcctg cctgaaccgt ttacgccacg accgcttgtt tgaggtcggc aaagacgatg
      301 atgccggcgc cggtttggag ctggctctga acctgcactt ccacctgctg gccaatggcg
      361 gtttgggcgt tgttgaccac gaccatcgtg ccgtccggca agtacccgac gccctggccc
      421 ttgtctttgc cctcgcgcac gatgcgcaag gagagtaatt cgcccggcaa caggattact
      481 cggagcgatt tggccaaatc gtgcaggttg acgtgattga ccttctgcag ttcggcgatc
      541 tggcccaggt tgtggtcgtt ggtatagagc ttcgcgccca ggttgcgcgc caggcgcacg
      601 agcttcgcat ccacttccgt ctcctctggg aaatcaccat cgtggatttt gacttcgttg
      661 cgcgtgttgc gctggatgcg attgagcacc tccagaccac ggcggccgcg agcacgcttg
      721 agggcgtcgt tcgaatcggc gatctgctgc agctccttca gcacaaagcg cggcacgacg
      781 attaagcctt cgacaaaatt cgcctcgatc agatcggcga tgcggccatc aatgatgaca
      841 ctcgtatcga gcagcaggag attgtccggt ttgttttgcg gtgcgaagcg gacatagggg
      901 atgatgagag agaagtcctc cttgttgctg cgcatggcca gcacgatgcc aatgtagcta
      961 aagctgagaa acaggctgag gcgaattaac cagcgagtgg gatgttcctc cacgttttcg
     1021 aacaggccgg agcgatcaat aaggagcgcg acgactgtgc ccaggagcag gccgaaggta
     1081 gtcgccgaaa aggcccgcag ggaaaagccc ttgagcatct cgtcgagagc gatcatcaat
     1141 ccaccgaagc caaagccgat gagcatcccc aggaaccagc cataggagct gcccacatac
     1201 tcgggccgca cctggctgac ggcgtagccg ccgatggtgc acatggacag aaatagaatt
     1261 cgaattaccc agagcgccat aatcagtcaa aaggattctt gggtgtggtc aggggtctca
     1321 agaccggccc aggtgtgttt gtgctgggag gcttgtgctg ccgcaggata ccccagaggc
     1381 ccaggcgatt cagattgctg gtggtgatgc tcaagttctc aacaacccgg gacacattgg
     1441 ccgcaatttg ctcgttttca agcaattgtc cagccaaacc tttgccctgc tgcacgccat
     1501 cgagcagatt cttcaaggta gtcgtggaag attcaatgtt tttcaccgcg gcgtcaatct
     1561 cgggcgagtt ggttgccacc aactcccgca cggcactggc ggccaggtta atttgctctg
     1621 taaagaagag aatgttgctg accgagacgt tgatggccgg gccattagtc gccacgatcg
     1681 cattgaggtt atccacgacg atcagagaac gttcggaaac ccgctggagg ttgtttaaag
     1741 tggcagagag attcgtcagg gcccgcgcgc tcaatacggt gcggcgggct tccatcaggg
     1801 catcgttgag gttggtggcg gctgaatcta tgtgctggag gaagccggcg gcaaagcggg
     1861 ccacttcctg gaggtcgagc ggcgcttcgg ccttggcctc gccgcccggg ccgaagaagt
     1921 ccccctcgtt gttggtcggc cggatggcga cgtattggtc gccgagaaag ccggattgct
     1981 cgatcacaaa gcgcgcgtcc ttgtgaatct gaggttgctt gtaaagccgg agggtgatgg
     2041 tcacgctctt tccgtcgggg gccagccgca tgtcggaaac cgtgcccacc tgcacgccgg
     2101 acatgagcac ctgggcgcgc gtcttcaagc cgcccacgct gctggcgttg agcaggatgt
     2161 cgtaagtggg gcggaagaac gagaggccct tgctgaactg caacagcagc acgaccagca
     2221 gtgccaggcc gatgaagaca aacaagccaa ccttccattc aagccgtgat ttgctcatat
     2281 tagaaataga gttctttggg atcagaaacg ccctcaataa accggcgcac gaccgggtcc
     2341 tgggaattga aaatctcgtc cggcgtcccg gtcgcgtaaa tcctttggtt gtgcaacatc
     2401 attatgcgct ggcccaggcg ccgcgcgctg cgcatgtcgt gggttaccac taccgttgtc
     2461 acgtctaacc ggtcgcgcac ccgcaggatg agcttatcta tgctgtccga cacaatcggg
     2521 tccagcccgg ttgtcggctc gtcatacagc acgatttgcg ggtgataaat aaccgcgcgc
     2581 gccagaccga cccgcttttt catgccgccc gacaattcag aaggtttctt gtcctcggtg
     2641 ccgggcaaat ccaccatttc gagcatctcg gccacctttt cccggatttc ctctttcggc
     2701 agcgagcgat cgcggcgaaa ggcaaacccc acattctccg ccacggtcat ggaatcgaag
     2761 agcgcggcgc tctggaacaa catgccgaac ttgtggcgca cgcgcagcag ctcccgctca
     2821 ttcatctgga cgatgttctc gccctcgatg agaacctgcc cggcgtcagg ttgcaggaga
     2881 ccgatcaggt gtttgagcaa gacactctta cccccgccgc tgcggccgat gatgacgacc
     2941 gactcgcctt tttcaatccg aaagctcacc ccgtccagga tggaatgggt gccaaagctc
     3001 ttcttcagat cgcggacttc gatcatagcg catggagaat ctggcccagg gtgagcgtga
     3061 gaaagaaatt ggagatcagg atcgtgattg aggaatatac caccgcctcg gtggtggcgc
     3121 gtcctacgcc ttcggcgcct tcaccgcaac tcaagccttt gtaacaactg atcagggcca
     3181 cgatgccgcc gaagatgact gccttgacca ggccgacgac gacatccgag ctgctggtgt
     3241 accgcagcat gttgtaccag gaatacgtcg gatcgatgcc gtacaagcgc acccctacga
     3301 ggtagcccgc accgatgccg acggcaatgc tctccgcagt taatagtggg agggcaatgt
     3361 gaagcgccag caagcgcggt acgaccagat agtccaccgg atgcgtggcc agtgtgcgca
     3421 aggcgtcgat ttgttcggtg acgcgcatcg tgcccaactc cgccgcgatg gccgcgccaa
     3481 cctggcccgc caccatcagc ccggtcagga ccggcccgag ttcgctgcac atggaaatac
     3541 tcaccaccgc aagcgtggca gtatccattt tgaccttgtg aaattgaagg taggtctgcg
     3601 cggcaagcac catgccggtg aacgcgccgg tgatcagcac caccgactgg gatttcacgc
     3661 cgacgaaata gatctggtag agcaggtcgc gccacgccac tttgaaggtc aagagcgatg
     3721 cgacggactc ttttgccagc aaggtgacgc ggcccaggcc ctccagccaa tcggcaatcc
     3781 agttcttacc ccaagaattc ataaccgcaa ccaaactagc aggtggccgg cgccagcgcc
     3841 agagtttgat tcgggcaggg cgtgccggga gcaactcatt tcttgtaccc ctcgcatgga
     3901 ggacgcgcgt ggcctcagaa aacggctgct tccacagtgg aatggggcct ctggcgggct
     3961 tgctctcacg gcactgttag cgtgttgctc gcccgctgca ctggcgatgc ttcgacggag
     4021 ttacgaagga gttacggtgg agttacgaag gaatggcctg gaaatcaggc gctggttacg
     4081 gtttctggac ggtctgggcg acgcaatgag ttggcttgca accacggcgc ttgccggggg
     4141 ttgaatgcca ctgtgccacc gcggccgctg cttagacgac cgagcttcgc cccggttcat
     4201 tccactcggt gcccattggg cgttcagaaa aggagatgct ctcgggcgtg ccgctaatgg
     4261 cagactgagc gacgctggcg gttgatcacg aaaagaccgc tgttctcttt cctgccggac
     4321 gaggtcatgg gatgggcgtt gctgtttatg ggactagcat cacgcggtaa aaccgctggg
     4381 gcctcccgac gaaggtatcg gtcaccacga tctgcgagcc ggtcgcggtg aggtcggcgc
     4441 ccagggttgt ccaagtgggc gcgtccaact ggtctttgaa ttcggtgcgg tacgtgcggc
     4501 ctggaatcgt gctggcttgg aaggcaaacg tcccgccagc ctggacgacg cacacgccaa
     4561 gctgaggcga aggcggcggg cccagacggt tgctggcgcg gggcgaccag tcggtgaaga
     4621 agtaaaccgc attcgtgttg ccgtcgggga agagcccctg gcttacattc tccaactgcg
     4681 cgccgaaagt gaccatgttc tgcatcgtgc cgttggccgc gaaacagcca agggtcatgc
     4741 cactctgagg aagcttgaag ttgacgtgaa gatcaccgtt gatgccggag ccgttcagct
     4801 cggtattctt gtccgcccaa accagtagaa agccgtgggg cgggatgacg gtgccggccg
     4861 gaacccgcga ctgagtggga ttcgagaggt cgtcggtaaa ataaaaaccg gagatgtccg
     4921 ccgcggagct gttcgggttg tagagctcga accagtcgct gaatgtgcca gcgaccgggt
     4981 cagcgaagcc gccgggcccg ttgttcttcg ccatccattc attgaaaacg accgggcgca
     5041 gagcggctgc gcccaggttg gtcacgacga tcgaaacgga agcgttggtg accagggcgc
     5101 cgtagttgtc gaaaccctgg acggccagga aattactgta gttgaacagt gacacgggca
     5161 cggtccagtt ggtgaggctc aaccaggtga tcgcaccgga gacggcgttg atgcggatat
     5221 ccttgaccgt gagtggagcg atgccgccca ggatgatcgg gttgttggtg gtggcgaacc
     5281 cccggccgcc attgttcgta atcgcgaagg ccgtggtcag gggcatcgcg gaacggatga
     5341 agttggcgcg ttgctggaga tagttgacca caccgctcca atcctgcccg agcaatccgc
     5401 tatagcgatt ggcccagggg ctgagatggg cactattgat agtcgtggtc atgaggtcca
     5461 ggaggtggtt gtagtaacgg cggtagttgt ccggcagagt aacgatgtta tacatgcccg
     5521 gggaagaggt gccggggaaa gccgcattca cgggcgcggc aaacgaaaaa tcctcgtccc
     5581 aaagcaggcc gagcgccttg ccatcgtcct gacggaaata gaccatgaaa ttatggttat
     5641 aaccggcggt gaacgtgtca acgtcgccgg taaaagcctt gaaggccagc atgcgcatcc
     5701 actcgtccga gtccatcagt cgagcggtct gggtctggag cgcggggccg gtgaggctga
     5761 aggacttgct gaaggtcatt agctgactgt aatcatctat gtcggtgttg ttttcctgga
     5821 agaataccca tcggtagttc tctgggtcgt tgccccagtc ctggatgtcc acattgacaa
     5881 cattgtccgg ttgcgggagt ttcggggcct gcgggtcgcc ggtgagggtg gtggtgggat
     5941 agtaaatgac ctcgagtttg tagcggttcc cattgccatt gttctgaaac tgctcatcga
     6001 agtagctccc gtcgaagccg gaaatgcgca acatcgcggt gccgttcaat tgcgggttgg
     6061 gggcgaaaac ctggaccagg tcgcactcga acccgagcaa tccgccggcg tggttgatcg
     6121 cgtgccaaag cagtatctcg ccatgagtgc cgcctaggcc agagtagccg ccggagcgat
     6181 ccatcgtgat cgtctccagg aagccgcgga agggttgctc gggatgcagc ttcacggtga
     6241 agccctggcg gttggggtcg tcgcgacccc gttcgctgct ctggagatga acgccgacgt
     6301 cataaaagac ctggcgctcg tcatgcacga cggttaggcc gatgagatcg ttgctcataa
     6361 cattggtgtc cgccagcagc agcgcggtgt ccgccggcgt catcagcaga cgcaaccggt
     6421 gaagctgggg catcagggcg gtgccatcat ccactttgaa cagggcgcgg gagtcacggc
     6481 cccgggcagg gaaggtggtg gaaaccccga ggttatcggc ggcttggacg tagaactgaa
     6541 ccagggcgcc ggccgtttgg ccggagatca agccggtata attggtgtag ccgggcgagg
     6601 cagccacttg ggcggaaagc atgggcgcct gctgccagac tcccccgttg accgaccacc
     6661 aaagtgtgac ggctcgaacc ccggctgggt cgctcgcact cacgttcacc gtaaccgcct
     6721 ggttgggctg tgggatgatc gggctgtggc cgaaggcggc aaaagtcggg cccaggtggg
     6781 cggtaaacgt ggagttgcgt ccacccgggg tgccatgttg ggacggcatg ggcaggacgg
     6841 tcgtcttcgc cacgcgattg aagtaaagcc gcgtgttcaa atggttattc cccgcgagcc
     6901 acttggcccg gaaggagatt tggtaagtct tgccattcgt gatgctgcga ccattggcgt
     6961 aagtcgtctc aaggtggttg tgcaaagtct cagtgctgcc tgtggccacg aggtgcaaga
     7021 cgtgtccggc ggggttgtcc gggtccgtct cgacccggct gtggctgtga ttgcccaggc
     7081 tgcgccaagc c
//
LOCUS       DATCGS010000538         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_195740, whole genome shotgun sequence.
ACCESSION   DATCGS010000538 DATCGS010000000
VERSION     DATCGS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195740"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIL39_11020"
     CDS             complement(<1..1041)
                     /locus_tag="VIL39_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5482005.1"
                     /translation="MLDNLLDYIPDGVFTVDAEWRVTFFNRAAEQITGIKRADAVGRR
                     CCDVFRASICENACALKQTLSTNRPVVNKVVYIIDARGQKIPISISTAALKDDRGHVV
                     GGVESFRDLRLVEALHRELQQQNSFSDIIGRSAAMRQIFELLPEIAESESAVLIEGAS
                     GTGKELFARALHNLSRRRGKKFVALNCGALPDNLLESELFGYKAGAFTDARRDKPGRF
                     AVANGGTIFLDEIGDISSAMQARLLRVLQERVYEPLGGVEPVKADVRVIAATNKSLGK
                     LVEQGRFREDLFYRIHVIRIELPSLRDRREDIPLLIDHFIARFNRLKGKDIDGVSDQV
                     LARLMEHGFPGNV"
     gene            1232..1786
                     /locus_tag="VIL39_11025"
     CDS             1232..1786
                     /locus_tag="VIL39_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY5482006.1"
                     /translation="MSEFKSAGSVRNEGGRWNPYLVGAGLGVLSWLVFAVVNTPLGIS
                     TPISSAASLCAIPAMGREAVARNAYWAKFPCKWDYHMLFLVGTFLGSLASVLASRTFR
                     LETVPATWAERFGGSKGGRLAAAFLGGVIIMFGARMAGGCTSGHGISGSLQLALSSWV
                     FFLILFGFGVITALILFPGGKTKT"
     gene            1799..2374
                     /locus_tag="VIL39_11030"
     CDS             1799..2374
                     /locus_tag="VIL39_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY5482007.1"
                     /translation="MTLPITGDTARWLAIPFGMLFGVLLHRGGVANYNVIVNQFRFRD
                     FTVLKIMLTAIIVGGIGVWALHGTGYAQYQIKPANLLGVALGAALFGIGMVLYGYCPG
                     TGVAAIATGSLHALVGFSGMLAGAVLYALSYPWVEKHIQPVAALGKVRLPEITGIPDL
                     GWFVILLAIAGAVFWLLERKTKPCGGPRRGN"
     gene            complement(2381..2791)
                     /locus_tag="VIL39_11035"
     CDS             complement(2381..2791)
                     /locus_tag="VIL39_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5482008.1"
                     /translation="MDEAERVFMRRKLTSMNWYIWIIIGAAIAVFLIFKQRTVVGRET
                     AREWLKKGATVIDVRSEAEFQEKHLPRAINIPLNRLGDEIARLAPNKEQPLLLHCQSG
                     TRSGMGEATLREMGYRNVLNLGSYGRARQILDSR"
     gene            complement(2784..>3208)
                     /locus_tag="VIL39_11040"
     CDS             complement(2784..>3208)
                     /locus_tag="VIL39_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5482009.1"
                     /translation="GMTVDQLSKVDLCYAPPYSPALDNIITAANVARNKLDGQVVGIT
                     PMEVKARLDANEVFVLLDVRSPAEHQQEHLAGSTLIPLGALRSRLGELPKEKEIVAFC
                     KVSLRGYEAAKILQGAGFKRVRVMDGGMAVWPYEKIRG"
BASE COUNT          580 a          993 c          934 g          701 t
ORIGIN      
        1 cacgttgcca gggaagccgt gctccatgag ccgcgccagc acttggtcgg aaacgccgtc
       61 aatatctttg cccttgagcc ggttgaaccg cgcgatgaaa tgatcaatca gcagcgggat
      121 gtcctcccgg cggtcccgca ggctcggcaa ttcaatgcga atgacatgaa tccgataaaa
      181 caaatcctct cgaaaccggc cttgctccac cagcttcccc aggctcttgt tggtggccgc
      241 gatgacgcgc acgtcggcct tcaccggttc caccccgccc agcggctcat agacccgctc
      301 ctgcaagacc cggagcaacc gggcctgcat cgcggaggag atgtcgccga tctcgtccag
      361 gaagatcgtg cccccgttgg ccaccgcgaa ccggccgggc ttgtcgcgcc gcgcgtcggt
      421 aaatgcgccg gctttgtagc cgaacagctc cgattccagc aagttgtccg gcagcgcccc
      481 gcagttgagc gccacaaact tctttccccg ccgccgcgac agattgtgca gggcgcgcgc
      541 aaataactcc ttgcccgtcc cgctcgcgcc ttcgatgagc accgcgctct cgctctcggc
      601 aatctcgggc agcagctcaa aaatctgccg catggccgcg ctgcgtccaa tgatgtcgga
      661 aaaggaattc tgctgctgaa gttcgcggtg cagcgcctcg accaggcgca agtcgcggaa
      721 gctctccacg ccgccgacaa cgtgcccccg atcgtccttc agtgctgccg tggaaatgct
      781 gatgggaatc ttttggccgc gggcatcaat aatatagacc accttgttga ccaccggccg
      841 gttggtggag agcgtctgct tcaacgcgca agcgttctcg cagatgctgg cccggaacac
      901 atcacagcag cggcggccca ccgcatcggc gcgcttgatc ccggtgattt gttcagcggc
      961 gcggttaaag aacgttacgc gccactcggc atccaccgtg aatacgccgt ctggaatgta
     1021 gtccagcagg ttgtccaaca tcgctcgaag cttaatcggg aggttgcgct ggtgccagtg
     1081 ccaaacgcga attgccacgg gtgttgcaat ttgccacacc gcgccggccc atccctattg
     1141 tgtcgtttgc agccacgcgg ctcagtttcc aaatgtatca agtgtaactt tttgcctcag
     1201 gggggctcta tagaaccgac gataacctct tatgagtgaa ttcaaaagcg ccggctccgt
     1261 cagaaacgaa ggcggccgtt ggaatcccta cctcgtgggt gcgggattgg gtgtgttgag
     1321 ttggctggta ttcgccgtgg tgaacacacc tctcggcatc tcgacgccga tctcttcagc
     1381 ggccagtctc tgcgcgattc ctgctatggg ccgagaggcg gttgcccgga acgcctactg
     1441 ggcgaaattt ccctgcaaat gggattatca catgttgttc ctggtgggga cgtttcttgg
     1501 cagcctggcc agcgtgttgg ccagccgcac tttccggttg gaaaccgtgc cggcaacctg
     1561 ggctgagcgg tttggtggtt ccaagggtgg ccggctggcg gcggctttcc tgggcggagt
     1621 gattattatg ttcggggcca ggatggctgg cggttgcacc agcggtcacg gcatcagcgg
     1681 ttcgttgcag ttggcgctga gcagttgggt gttcttcctg atactgttcg gctttggcgt
     1741 gatcacggcg cttatcctgt ttcctggcgg caagaccaaa acgtaacccc cactactcat
     1801 gacacttccc attactggtg acaccgcccg ctggctcgcg ataccgttcg gcatgctctt
     1861 tggcgtgctg ttgcaccggg gcggcgtggc gaattacaac gtcattgtga accagttccg
     1921 cttccgggat ttcacggtcc tgaagatcat gctgacggcc attatcgttg gagggattgg
     1981 cgtgtgggcg ctgcacggca cgggatacgc gcaataccaa atcaagccgg ccaatctcct
     2041 gggcgtggcg ctgggcgcgg cactttttgg catcggcatg gtgctctacg gctattgccc
     2101 cggcacgggg gtcgcggcga tcgccaccgg gagcctgcat gcgttggtgg ggttctccgg
     2161 tatgctcgcg ggggcggtgc tttatgccct gagctatccg tgggtcgaaa agcacatcca
     2221 acccgtcgct gcgcttggaa aagtgcgtct gccggaaatt acaggtattc cggacttggg
     2281 ttggttcgta attctcctgg cgattgcggg cgcggtgttt tggctcctgg agcgaaaaac
     2341 caagccatgc ggaggtcccc gcaggggtaa ttgactgcgc ctaccggctg tcaagtattt
     2401 gcctcgcccg tccgtatgag ccgaggttca atacgttgcg ataacccatt tctcttagcg
     2461 tggcctcccc catgccgctt cgtgtcccgc tctggcaatg caacagcagc ggctgctctt
     2521 tattgggagc cagccgcgca atctcatcgc ccaatcggtt gagggggatg tttattgccc
     2581 tcggcagatg cttttcctga aactccgcct cgctgcgcac atcgatgact gtggcgcctt
     2641 ttttcaacca ttcgcgcgcc gtctcccgcc ccaccacggt ccgctgcttg aagatcagga
     2701 agaccgcaat cgcagccccg atgatgatcc agatgtacca attcatagac gtcagcttgc
     2761 ggcgcataaa cacccgttca gcttcatcca cggatctttt cgtacggcca caccgccatg
     2821 cccccatcca tgacccggac gcgcttgaat cccgcccctt gcaggatttt ggccgcttca
     2881 tagcccctca gggaaacttt gcagaaagcg acgatctcct tctccttcgg caactcaccc
     2941 agtcgcgagc gcagcgcgcc caagggaatc agggttgagc cggctagatg ttcctgctga
     3001 tgctccgccg ggctgcgcac atccagcagg acaaagactt cattcgcatc cagccgcgct
     3061 ttgacttcca tcggcgtaat gcccacgacc tggccatcga gcttgttgcg ggccacgttc
     3121 gcggccgtaa tgatattgtc cagtgcgggc gaatagggcg gcgcgtaaca cagatcgacc
     3181 tttgaaagct gatccacggt cattcccg
//
LOCUS       DATCGS010000539         3238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_491976, whole genome shotgun sequence.
ACCESSION   DATCGS010000539 DATCGS010000000
VERSION     DATCGS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491976"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VIL39_11045"
     CDS             <1..556
                     /locus_tag="VIL39_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEY5482010.1"
                     /translation="HFLENHDEPRIAGILSLAEHRAAALLILGLPGMRFLHDGQLSGA
                     RLKIPVQLARRAEEPSQAEMVNLYEQLLTTLPGTAVGQGRGELSKPRAAWPGNSTAQN
                     FVIVQWQKQAPEFDLVVVNLAPNRSQCYAPLSLEGLAAHNWAMTDLLGEERFKRSGDD
                     LQNQGLYLDLPPHGAQLFHFEAIN"
     gene            773..>3238
                     /locus_tag="VIL39_11050"
     CDS             773..>3238
                     /locus_tag="VIL39_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY5482011.1"
                     /translation="MTIRADIKNERCRVNRTGPPPKQGLYDPQFEHEACGVGFVVNIK
                     GRKSHAIIQQSLQVLLNLNHRGACGCEANTGDGAGILIQPPHEFLKLVAREARFTLPS
                     AGEYGVGMVFLPQDAAQRGECEKIFAGIAAEEGQPVLGWRTIPTSNVTLGSTALASEP
                     LMRQVFLARNAKLTDDLAFERKLYVIRKRAENAIRYSGKVAGGDFFYVSSLSCKTVVY
                     KGMLLTEQLDQYYPDLSHPAMESALALVHSRFSTNTFPSWNRAHPYRYIAHNGEINTL
                     RGNINWMHARQAMFESDLFGDDLKKVLPVICTDGSDSAIFDNCLELLVLAGRSLPHAM
                     MMMIPEPWTRHESMSAEKKAFYEYHSCLMEPWDGPASIAFTDGRQIGAVLDRNGLRPS
                     RYYVTKDDLVIMASEVGVLDIPADRVLQKGRLQPGRLFLVDTEQGRIVADEEIKHQVA
                     TAHPYREWLDQHLIRLADVPDPVIPILLEPNHETVIERQLAFGYTFEDLRILMLPMAR
                     DGAEAVGSMGTDTPLAVLSNKAQPLSSYFQQLFAQVTNPPIDCIREEIVTSTVTAIGS
                     ERNLLQPSPEHARLVELKSPILTNEEFAKLKHIHLPGFKSITCPILFPAADGAPGLEK
                     AMDDLCAKVSQAIKDGINVVVLSDRGVNKEWAPIPSLLAVAGLHHHLIREGTRTQVGL
                     VLESGEPREVHHYSLLIAYGCGAINPYLAFETLDDMLRQGLLTGIDHKTACKNYFKAA
                     MKGVVKVLSKMGISTIQSYWGAQIFEAVGLKQSFIEKYFTGTPSRIEGVGIGVIAQEV
                     LLRHQRAFPDRPVNGHLLDAGGQY"
BASE COUNT          625 a         1055 c          948 g          610 t
ORIGIN      
        1 ccactttctg gagaatcacg acgagccgcg catcgctggc atcctgtcgc tcgccgagca
       61 tcgggctgcg gctttgctca ttctgggctt gcccgggatg cgcttcctgc atgatggcca
      121 actctccggg gcgcgcctca agattccggt tcaactggcg cgccgcgccg aggaaccaag
      181 ccaggccgag atggtgaacc tctatgagca attgctgacg acgctgccgg gcactgcggt
      241 cgggcagggg aggggtgaac tatccaaacc gcgcgccgcc tggccgggca attccaccgc
      301 gcagaacttc gttatcgtgc aatggcaaaa gcaggcgccg gagttcgacc tggtggtcgt
      361 gaatctggca cctaatcgca gccagtgtta cgcgccgctc agcctcgagg gcttggcggc
      421 gcacaactgg gcgatgaccg acctgctggg cgaggaacgc tttaaacgct ccggggatga
      481 cttgcagaac cagggcctct atctggacct gccgccccac ggtgcccagt tgttccactt
      541 cgaagcaatc aactgagcca ggggatacat gggatataca agggatagat aaggggtgta
      601 cgagcgagca acgcgctcgc aataccgggg caaccctgta gcaccaccat ggctggagcc
      661 ctgtctgcgc ccagcgcaat tattttaagg ccagtattgc atccggcgct atttcgggta
      721 tatactactc aactttagtt acagttaacc tggagacttg catctcccga agatgacgat
      781 tagagcagac attaagaacg agcgctgtcg cgtgaatcgg accgggcccc cgcccaaaca
      841 gggtctttac gacccgcagt ttgagcatga agcctgcggg gtggggtttg tggtcaatat
      901 caaggggcgg aagtcccatg ccatcattca gcagtcgcta caggtgctgc tgaacctgaa
      961 tcaccgcggc gcgtgcggtt gcgaggccaa caccggggat ggtgcgggca ttcttatcca
     1021 gccgccgcac gagttcctca agctggtcgc ccgcgaggcg cggttcacgt tgccctctgc
     1081 cggcgaatac ggggtcggca tggtgttcct gccccaggac gccgcccagc gcggcgaatg
     1141 cgagaagatc tttgccggca tcgccgccga agaaggccag cccgttctcg gctggcgcac
     1201 catcccgacc agcaacgtca ccctcgggtc cacggccctg gcctcggaac cactcatgcg
     1261 gcaggtcttt ctcgcccgca acgcgaagct cacggatgac ctggcgtttg agcgcaagct
     1321 ctacgtgatc cgcaagcgcg cggagaatgc catccgttac tccggcaagg tcgcgggggg
     1381 cgacttcttc tacgtgtcca gcctgtcgtg caagacggtc gtttacaaag gcatgctgct
     1441 caccgagcaa ttggaccagt actaccccga cctgtcgcac ccggcgatgg aatccgccct
     1501 ggcgctggtg cattcgcggt ttagcaccaa taccttccca agctggaacc gcgcccaccc
     1561 ctaccgctac atcgctcaca atggcgagat caacaccctg cggggcaaca tcaactggat
     1621 gcacgcccgc caggcgatgt tcgagtccga tctcttcggc gatgacctca agaaggttct
     1681 gcctgtgatt tgcaccgatg gcagcgattc ggcgatcttc gacaattgcc ttgagctgct
     1741 tgtcctggcc ggccgttcgc ttccgcacgc catgatgatg atgatcccgg agccgtggac
     1801 ccggcacgag agcatgagcg ccgaaaagaa ggccttttac gagtatcact cgtgcctgat
     1861 ggagccctgg gacggcccgg cttccattgc tttcacggat ggccggcaga tcggtgcggt
     1921 gctcgaccgc aatggtcttc gcccctcccg ctactacgtc accaaagacg acctggtcat
     1981 catggccagt gaggtcggcg tgctcgatat ccccgccgac cgcgtcttgc agaaaggccg
     2041 cctgcagccc ggccgcctgt ttctcgtgga taccgaacag ggccgcatcg ttgcggatga
     2101 ggaaattaag catcaggtcg ccacggccca tccctaccgc gagtggttgg accagcacct
     2161 gatccggttg gccgacgtgc ccgacccggt catccccatc cttctggagc cgaaccacga
     2221 aaccgtgatc gagcgccagc ttgcctttgg gtacaccttt gaagacctgc gcatcctgat
     2281 gctgcccatg gcccgggacg gcgccgaagc cgtgggctcg atgggcacgg acacccccct
     2341 ggcggtcctg tccaacaagg cgcagccgct ctccagctac ttccagcagc tcttcgccca
     2401 ggtcaccaat ccgccaattg actgcatccg cgaggaaatc gtcacctcca ccgtgacggc
     2461 tattggctct gagcgcaacc tgctccagcc ctcgcccgaa cacgcccggc tggtcgagct
     2521 caagtctccc attctcacca acgaggaatt cgccaagctc aagcacattc acctgccggg
     2581 cttcaagtcc atcacctgtc cgatcctctt cccagccgcc gacggcgcgc ccggcctgga
     2641 gaaggccatg gacgacctgt gcgccaaggt cagccaggcc atcaaggacg gcatcaacgt
     2701 ggtggtcctg tcggaccgcg gcgtgaacaa ggaatgggcg ccgatcccgt cgctgctcgc
     2761 ggtggccggc ctgcatcacc acctgattcg tgaaggcact cgcacccagg tgggcttggt
     2821 gctcgaatcc ggcgagccgc gtgaagtgca ccattattcg ctcctcatcg cctatggctg
     2881 cggggctatc aacccttatc ttgccttcga gaccctcgac gacatgctgc ggcaggggct
     2941 tttgacgggc attgatcaca agacggcgtg caagaattac ttcaaggccg cgatgaaggg
     3001 cgttgtgaag gtgctctcga agatgggcat ctccaccatc caaagctatt ggggcgcgca
     3061 gatattcgaa gcggtcggct tgaagcagtc ctttatagaa aagtacttca cgggcacgcc
     3121 ctcccgcatc gaaggcgtgg gcattggagt cattgcccag gaagtcctcc tccggcacca
     3181 gcgcgccttc ccggaccgcc cggtcaacgg ccatctgctc gacgccggcg gccaatac
//
LOCUS       DATCGS010000540         4531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_689693, whole genome shotgun sequence.
ACCESSION   DATCGS010000540 DATCGS010000000
VERSION     DATCGS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689693"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VIL39_11055"
     CDS             <1..243
                     /locus_tag="VIL39_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482012.1"
                     /translation="KGGTPNLPQRVSMLFFKQALSILFVFSWCSLGVLPLSPSPRYSK
                     GVSFGARLFGAQTFRISQMGGVGLGLRGACQPAETD"
     gene            253..1356
                     /locus_tag="VIL39_11060"
     CDS             253..1356
                     /locus_tag="VIL39_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY5482013.1"
                     /translation="MKFNCSLCALVVLLVASVVQSPGQTTDAERKQFEAVRAKADKGD
                     ADAQLSVAARYASGDGVARDLAKATKYTRKAAEQGLARAQCLLGLLYSNGDGVKVDKA
                     EAARWFHRAADQGVAEAQFDLGMCYANGEGVARSAAVAAEWYRKAAAQDLPEAEGELG
                     NCYLEGNGAPTDIPEGLKWTRMAADQGFAPAQNTLGLCYSKGKGVAKDYVEAYKWFNL
                     AVAKGGELADDVKINLAAAERFLTPEQVADAQRMAREFKPRKASAPGATLTPPDKASS
                     VPAGRETGQPGSVGGASAKALKTGVVNVKAEDESCEIFVDGAFVGNTPANVKLPEGAH
                     VVEVKKPGFKDYRKQIAITEGSELTLRAVLEKQ"
     gene            1456..2616
                     /locus_tag="VIL39_11065"
     CDS             1456..2616
                     /locus_tag="VIL39_11065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5482014.1"
                     /translation="MKVPLLDLKAQYAAIKTEVDASVAEVMESQQFILGPQVRKCEQA
                     IAQYSGCAYGIGVSSGTDALLVCLMAEGIGPGDEVITTPYTFFATAGSVARVGAKPVF
                     VDIDPASYNLDPAQIESKITAHTRAIIPVHLYGQMADMAAVMQVANAHKLLVIEDAAQ
                     AIGAECKDRRAGSIGHYGCFSFFPSKNLGAAGDGGMVVTQDAQRAEKVIRLRNHGAQP
                     KYYHKVIGGNFRLDTLQAAVVLAKLRHLDNWTAARQRNADRYNRLFGAAGLETADSSS
                     PHPKRNGSRRVLLPKIVTGRHIFNQYVIRVARRDQLKAALQEKGVGTEIYYPLPLHLQ
                     ECFSYLGHAAGAFPESERAAQETLALPVYPELSEEQARYVVQCITEFLSTGA"
     gene            2660..4291
                     /locus_tag="VIL39_11070"
     CDS             2660..4291
                     /locus_tag="VIL39_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEY5482015.1"
                     /translation="MRRSLILAVVFLAAVAGAVAPETGPAIFESSGAVTPGGQIDKLV
                     LARLSPLGIQPALCSDAVFVRRVYLDVIGTLPTAKEAREFIEDPDTKNKRRLLIDRLL
                     ERDEFADSCALKWGDILRIKAEFPVNLWPNAAQAYYRYVRASIAANKPYDKFAREMLT
                     SSGSNFRVGSVNFYRAIQNRNPEGIATAVALTFMGVRADKWPTNQLAGMAAFFAQIGY
                     KPTREWKEEVVFWDPLNSSAQVTNTAPADSTLLPTNCTPEMATFPNGKQIKLPPDRDP
                     REVFAGWLITPENPWFTRNIANRVWSWLLGRGIIHEPDDIRDDNPPSNPALLAYLEKE
                     LVAGHYDLKRLYRLILNSKTYQFSSVPRSNVPQAEANFGSYALRRLDAEVLIDAINKI
                     TGSSDLYTSAIPEPFTYIPADEPAIALADGSITSPFLALFGRSARATGMENERNNKVM
                     PAQCLHLLNSSHIQRKLEQSPKLKAIFDSGRKQPELVEELYLTILSRFPTPEEVKALE
                     EYGRPGKSGLRVKSREDWLDITWALINSTEFMYRH"
     gene            4320..>4531
                     /locus_tag="VIL39_11075"
     CDS             4320..>4531
                     /locus_tag="VIL39_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482016.1"
                     /translation="MSQAHQSKRPSPAADALCMTLSMPMSRREVMRAGFFSAAGLLLA
                     GGHVFGATNEPSPAKPLAAAPGKIKA"
BASE COUNT          938 a         1353 c         1368 g          872 t
ORIGIN      
        1 aaaggcggga ctccaaacct gccgcagcgg gtctcaatgc tcttttttaa acaggctctt
       61 agtattctct tcgtattctc ttggtgttct cttggtgttc ttccgctgtc cccctcgcca
      121 cgctattcca agggggtgag cttcggcgcg cgcctcttcg gggcgcaaac tttccggatc
      181 agccaaatgg gaggtgtcgg cttgggttta cgcggcgctt gccagccggc tgaaacggac
      241 taatctggca gtatgaagtt caattgttcg ctctgcgcac tcgtcgtact cctggtggct
      301 tctgtggtcc aatcgcccgg ccagacgacc gacgccgagc gcaaacagtt cgaggccgtc
      361 agagccaagg cggacaaggg cgatgccgac gctcaactca gcgttgctgc ccgttacgcc
      421 agcggcgacg gcgtggccag agacctggcg aaggcgacca aatatacccg caaggcggcg
      481 gagcaggggt tggcccgcgc ccagtgcctg ctgggtttgc tctattccaa tggcgacggg
      541 gtgaaggttg ataaagctga ggcggctcgc tggtttcacc gggctgcgga ccagggcgtg
      601 gccgaagctc aattcgacct gggcatgtgc tatgccaacg gggagggtgt ggctaggagt
      661 gctgccgtgg cggcggagtg gtatcgcaag gccgcggctc aagacctgcc ggaggccgaa
      721 ggcgaactcg gcaactgtta cctggaaggc aatggcgcgc cgacggatat tccagaagga
      781 ttgaagtgga ctcggatggc cgcggaccag ggatttgccc cggcccagaa caccctcggc
      841 ctttgctatt ccaagggtaa gggcgtggcc aaggattatg tcgaagctta caaatggttc
      901 aaccttgcgg tggccaaagg cggcgaactg gccgatgatg tcaaaataaa cctggccgcg
      961 gccgagcggt ttctgacgcc agagcaggtg gccgacgcgc agcggatggc gcgcgagttc
     1021 aagccgcgca aagcctcagc gccgggcgca actctcaccc cgccagacaa ggcgagttcc
     1081 gttcctgccg gcagggagac cgggcaaccc ggcagcgtgg gcggcgcgtc tgccaaagct
     1141 ttaaaaactg gcgtcgtgaa tgtgaaagct gaggacgaat cttgcgagat cttcgtggat
     1201 ggggcgttcg tgggcaatac gcccgcaaat gtgaagcttc ccgagggcgc gcacgttgtc
     1261 gaagtgaaga agccggggtt caaggattat cggaaacaga tcgcgattac cgaaggctcc
     1321 gaactgaccc tgcgggcggt cctggagaag cagtaatacc ccgattagac gagatggtcc
     1381 tgccccgtaa agactaaaga cgtgatcccc ctcgagacat aatggccgtt gatcgggcca
     1441 ctacttttgc aacacatgaa agtcccattg cttgacctca aagcgcagta tgcggccatt
     1501 aagactgaag ttgacgcctc ggtggccgag gtgatggaat ctcagcagtt catccttggg
     1561 ccacaggtgc ggaagtgcga acaggctatt gcccaatact caggctgcgc ctacgggatc
     1621 ggagtctcct ctggaaccga cgcgttgctg gtttgcctga tggcggaggg gattggcccg
     1681 ggcgacgagg tgatcaccac cccatatacc ttttttgcaa ctgccggctc agttgcgcgc
     1741 gtgggggcga aacctgtctt cgtggacatt gatccggcca gttacaacct ggatccggcg
     1801 cagatcgagt cgaagataac cgcccatacc cgcgcgatca ttccggtcca cctttacggc
     1861 caaatggccg acatggctgc cgtgatgcag gtcgctaacg cgcacaagct actggtcatt
     1921 gaagatgccg ctcaagccat tggcgccgag tgcaaggacc ggcgcgccgg ttccattggc
     1981 cactacggct gcttttcctt cttcccgtca aagaacctcg gagccgcagg cgatggagga
     2041 atggtggtga cccaggatgc gcaacgggcg gagaaggtca tccggctgcg caatcatggg
     2101 gcccagccca agtattacca caaggtgatt ggcggcaatt tccgcctgga taccctccag
     2161 gccgcagtcg tcttggccaa actgcggcat ttggacaact ggacagccgc ccgccagcgc
     2221 aacgcagacc gatacaaccg cctctttgga gcagccggcc tggaaaccgc cgacagctct
     2281 tcaccccacc cgaagcggaa tggctcacgg cgcgtgctcc tgccgaagat cgtgacgggt
     2341 cgtcacatct tcaaccaata tgtgattcgt gtggcacgga gggaccaact gaaagctgca
     2401 ctacaggaaa agggcgtagg aactgaaatc tactatcccc tgccgctgca ccttcaggag
     2461 tgcttttcct atctgggtca tgcggctggc gcgtttcccg agagcgagcg tgcggcgcag
     2521 gaaaccttgg cgctgcccgt atatccggaa ctcagtgagg agcaagctcg gtacgtagtg
     2581 cagtgcatca ctgaattcct gtccactggc gcatgattct tttcccatag ctttccgtat
     2641 gctgagatgg gaaaccataa tgagaagatc attgattctg gcagtcgtct tcctggcggc
     2701 tgtggccggg gccgtcgcac ctgagacggg accagccata ttcgaaagct cgggcgctgt
     2761 gacccccggg ggccagattg acaagctcgt tttggccaga ttgtcgccgc tgggcattca
     2821 acccgccctc tgctccgatg cagtgttcgt ccggcgggtc tatctggatg tcatcggcac
     2881 cctgcctacg gcgaaagagg ctcgggagtt tattgaagat cccgacacga agaacaagcg
     2941 ccgcctgctg attgatcgtc tcctggaacg ggatgaattc gccgactcct gcgcactgaa
     3001 atggggcgac attttacgca tcaaggcgga gttcccggtc aatctgtggc ctaatgcggc
     3061 ccaggcttac taccgttatg tccgggcgtc tatcgccgcg aacaagccct acgacaagtt
     3121 tgcccgggag atgctcacct cgagcggtag caacttccgc gttggctcgg tcaacttcta
     3181 ccgcgccatc cagaaccgga atccggaagg catcgccacc gcggtggcct tgacgttcat
     3241 gggcgtgcgg gcggacaagt ggccgaccaa ccagttggcg ggcatggcgg ccttcttcgc
     3301 gcagatcggc tacaagccca cccgcgagtg gaaggaggaa gtcgtcttct gggacccgct
     3361 caattccagc gcccaagtta ctaacactgc gcccgcggat tcgaccttgc tgccgaccaa
     3421 ctgcacgccg gagatggcga ccttcccgaa tggcaaacag atcaagctgc ccccggatcg
     3481 cgatccgcgg gaggtgttcg ccggttggtt aattaccccg gagaacccct ggttcacccg
     3541 taacatcgcc aaccgtgtct ggtcctggct gctggggcgg gggattatcc acgagccgga
     3601 cgatatccgc gacgacaacc cgccgagcaa ccccgcattg ctggcctatc tggagaagga
     3661 gttggtcgcg ggccactacg acttgaagcg cctctaccgg ttgatcctca actcaaagac
     3721 gtatcagttc tcctcggtgc cgcgctctaa tgtcccccag gctgaggcca acttcgggag
     3781 ctatgcgttg cggcgcctgg atgcggaggt gctgattgat gccatcaaca agatcaccgg
     3841 ctcctcggac ctctacacca gcgccatccc ggagcccttc acctacattc cagcggacga
     3901 accggcaatt gccctggcgg atggcagcat caccagtccg ttcctggctc tgttcggacg
     3961 ttcagcccgg gccacgggca tggaaaacga acgcaacaac aaggtgatgc ctgcgcaatg
     4021 tctccacctg ctgaattcga gccatatcca gcgcaagctg gaacagagcc ccaagctcaa
     4081 agccattttc gattccggcc gcaaacaacc tgaactcgtg gaagagcttt acctgaccat
     4141 cctgtcgcgg ttcccgaccc cagaggaagt gaaagccctg gaggagtatg gaaggccggg
     4201 aaagtcgggg ctgcgcgtga aatcacggga ggactggctg gacatcacct gggctttgat
     4261 taacagcacc gaattcatgt atcgccacta accaccccca accaaaagaa aggcacatta
     4321 tgagccaagc acaccagtcc aaacgcccgt ctccggctgc ggacgcgctg tgcatgacct
     4381 tgagcatgcc gatgtcccgg cgtgaagtca tgcgcgccgg cttctttagc gccgccgggt
     4441 tgttgttggc cggtggccat gtgtttgggg ccacgaacga accgtcgccg gccaaaccgc
     4501 tcgctgcagc gcccggcaag atcaaggcca a
//
LOCUS       DATCGS010000541         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_689705, whole genome shotgun sequence.
ACCESSION   DATCGS010000541 DATCGS010000000
VERSION     DATCGS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689705"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..361
                     /locus_tag="VIL39_11080"
     CDS             8..361
                     /locus_tag="VIL39_11080"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY5482017.1"
                     /translation="MSLGTTSHFEGIILAQTSISLATGASINGRLLAQTAVTLDSSTV
                     TTPAAAAAASTLVSAAVVRGPYVDAIGQSLNPTTKTITAPWSGSMQFYRIRSGTALTI
                     TSIAISGGNVVITYN"
     gene            complement(618..1043)
                     /gene="rnpB"
                     /locus_tag="VIL39_11085"
     ncRNA           complement(618..1043)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIL39_11085"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1163..2515)
                     /locus_tag="VIL39_11090"
     CDS             complement(1163..2515)
                     /locus_tag="VIL39_11090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5482018.1"
                     /translation="MLSATAAQRTFARPDEMRGAEQLVAASRPGTPANLKLTGDWDVL
                     VTVKKPRALATSVHVAPPEILAVVAEKYDSLPQFNNNAGGWAKGAQLRAVCAQETTTP
                     YLLDPESLVLRAGPEPDAPLFRRGEDYEADLVWGTIGRSAHSAIKETQPVFASYRHAR
                     LRIDAVVLTRKGKIVLRQGEPRSAAPLPPTIEKGERCLANIWLPGRLNQLGPEHLFPV
                     LETAYPEPPRARPAPARQLLPGTMKKLRSGQRLRILAWGDSVTVGTYVPDWEHNRWQE
                     QFVARLKARFPRANIELQTEAWGGRNTDSYLAEPPGSPHNYREKVLAAKPDLIVSEFV
                     NDAGLNPAQVHERYGKLLADFQGIGAEWIILTPHYVRPDWMNLTRERDVDDDPRPYVT
                     GLREFAARNRTALADASRRYGRLWRQGIPYSTFLLNSINHPNARGMKLFADALMELFP
                     "
     gene            complement(2871..>3724)
                     /locus_tag="VIL39_11095"
     CDS             complement(2871..>3724)
                     /locus_tag="VIL39_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY5482019.1"
                     /translation="RNWLPKAFTYWFDEPAPKDFEFVVDGMKRIKAAAPGIRRMLTVQ
                     PEPPLLGQVDIWCGLTPKWTPELVRERRVAGEEVWWYICCGPPAPYITEFIDHPGTEL
                     RLWPWQSWQYGVQGILIWATLYWTSPAAFPPPKLQDPWADPMSYKSGYDEKPGEIGYW
                     GNGDGRFLYPPRRDASSDPAPCLDAPVNSLRWENLRDGMEDYEYFWLLQQAATRELAW
                     HGDTGLLREALELLKVPQEVSQDLTHFTTDPRLMLGHRDRVARMIERLQSGAGWSVRQ
                     GAAWSAR"
BASE COUNT          681 a         1193 c         1123 g          727 t
ORIGIN      
        1 cggtgagatc agccttggaa caaccagtca ctttgaggga ataatattgg cccaaacctc
       61 gatcagcctt gcgacgggcg catcgattaa cggcagactg ttggcacaga ctgcggttac
      121 ccttgactcc agtaccgtta caaccccagc ggcggcggcg gccgcgtcca cattggtatc
      181 agcggcagtg gtcaggggtc cgtacgtgga cgctatcggg caatcactta atccaacgac
      241 taagaccatc acggctccct ggtcaggcag catgcagttc tatcgtatcc ggtcagggac
      301 agcgcttacg attacaagca tcgcaatttc tggtggtaac gtggtaatca cctacaattg
      361 aacaaatggg gtatccaaac cgtcttttcg agcttcctga tgagcgtcct gggcttgaag
      421 accgattcgc gccgcccgcc aaacacctaa ccgggatatg gggaacgact gagcacacgg
      481 ttcgataaca aacgtgagct tggtgcgggg agatcggaga gctttcgccg tcgttcgcgt
      541 tacacgccca gccagcctcc gctggtcacg tttcatgcat cacacctccg tccaccgccc
      601 gggcccgggc agtagctgat tttggaaggg ctgtaagccg atttttgtct gccccgaaac
      661 ctttcggagg agaggatcat ttgtctcagc agccaatacc cggaacctgt tccgctgggc
      721 ggaacgtgga gcgggccgct ccggggtccc ctatttggcc ttgcacccga tggggttttc
      781 cgtgcctccg cgcttacgct tggagcggtg ggctcttacc ccaccttttc acccttaccc
      841 cgccctcgtt tccgaggccg aggcggttta attttctgtg gcactatccg tcgggacgcc
      901 tcgcggcatc gcctcccgcg tgtatctcgc cgcaggcttt cttgcgtcag ccccggccag
      961 ttacgcggca tcgcgcccta tggagttcgg actttcctcc tcccccgaat tgcttcgggg
     1021 aaagcgatcc tccgcccttc caaaatcagg atgaatatac attgccggcg gcaggataac
     1081 aaggctgatc tcggacggcg cgtcaacagc gtttggctcg gtcgtttagc ccaggttgtg
     1141 ctcgctctcc tggcgttgcc actcagggga acagttccat caatgcatca gcgaacaact
     1201 tcatgccgcg ggcgttggga tggttgatgg agttgagcag gaacgtgctg taaggaatgc
     1261 cttgccgcca gagccgtccg taacggcggg acgcgtcggc cagcgcggtc cggttccgcg
     1321 ccgcaaactc gcgcaggccg gtgacgtaag gacgcgggtc atcgtccaca tcccgctcgc
     1381 gggttaggtt catccaatcc ggccgcacat aatgcggtgt gagaatgatc cactcggcgc
     1441 cgatgccctg gaaatccgcc agcaacttcc cgtaacgctc gtgcacctgc gccgggttca
     1501 ggcccgcatc gttgacaaac tccgacacga tgaggtcggg ctttgccgcg agcacctttt
     1561 cccggtaatt gtgcgggctt ccgggaggtt cggcgaggta gctgtccgtg tttcgcccgc
     1621 cccaagcctc ggtttgcagc tcaatgttcg cccgcggaaa acgcgcctta agccgagcca
     1681 caaattgttc ctgccagcga ttgtgctccc agtccggtac gtaagtgccc accgtgacac
     1741 tgtcgcccca tgccagtatg cgcaggcgct gaccggatcg cagtttcttc attgtcccgg
     1801 gcagcagttg cctggcgggc gcgggcctgg ccctgggcgg ctcgggatag gcggtttcca
     1861 gcaccgggaa aagatgctcc ggcccgagct ggttcagccg gccagggagc cagatattcg
     1921 ccaggcaacg ctcgcccttc tcgatcgttg gcggcagcgg cgcggcggag cgcggctcgc
     1981 cctggcgcag aacgatcttc cccttgcgcg tcagcaccac cgcgtcaatg cgaagccggg
     2041 catggcggta gctcgcaaac accggctgcg tttccttgat agcactgtgg gcgctgcgac
     2101 cgatggtgcc ccagaccaga tcggcttcgt aatcctcgcc ccgccgaaaa agcggcgcat
     2161 ccggttccgg cccggcccgc agtaccaagc tctcgggatc gagcaagtag ggagtggttg
     2221 tttcctgagc gcatacagcg cgaagctgcg cccctttggc ccagccgccg gcattgttgt
     2281 tgaactgggg cagagagtca tacttctcgg ccacgacagc caggatctcc ggcggggcaa
     2341 cgtggaccga agtggcgagc gcgcgcggct tcttgacggt caccagcacg tcccagtcgc
     2401 ccgtgagctt cagattggcc ggggttccgg ggcgactggc cgccaccaat tgttcggccc
     2461 cacgcatctc gtccggtcgg gcgaaagtcc gctgggccgc cgtcgcgctc agcataaggg
     2521 tcgggactcc cgcaagcatc aaagctcgag taagccccag gcgaacggtg cggaccctgg
     2581 gcattctgac agaggcgctt ttcattagtt cagtcgctga catcgaagcg agattagcag
     2641 ggattgcttg agattagcaa acctgagacc gctgactccg cagcaagaag cgcatccggc
     2701 gactagggcc ttccgtaatc gcccggaggc tgcttccgta atcgggcgac ttgttgggcc
     2761 gccacgccgg acccataatt gcatccatgc aaggtcgagc tcgattcgct tggcgagagg
     2821 aaagtggaca cgatggccag tccccggcat tgcgatggct acgcgccggg tcaacgcgcg
     2881 ctccaggccg caccttgcct cacagaccag cctgctcccg actgcaggcg ctcgatcatc
     2941 cgcgccacgc ggtcgcggtg gcccagcatg aggcgcgggt cagtggtgaa atgcgtcagg
     3001 tcctgggaga cttcctgcgg caccttgagc agctcaagtg cctccctcag cagcccggta
     3061 tcgccatgcc aggccagttc acgcgtggcg gcctgttgca gcagccagaa atactcgtag
     3121 tcctccatcc catcgcgaag gttctcccaa cgcagagagt ttacaggggc gtcaaggcag
     3181 ggcgccgggt cactgctcgc gtcgcggcgc ggcggataaa gaaaccgacc gtcgccattg
     3241 ccccagtagc caatctcgcc cggcttctcg tcgtagccgg acttgtagct catcgggtca
     3301 gcccacggat cctggagttt gggcggtggg aacgccgccg ggctggtcca gtaaagcgtc
     3361 gcccagatca ggattccctg cacgccgtat tgccaggatt gccacggcca gagccgcaat
     3421 tccgtacccg gatgatcaat gaactcggtg atatagggtg cgggtggccc gcaacagata
     3481 taccaccaga cctcctcgcc tgccacccgc cgctcgcgca ccagctcggg ggtccatttc
     3541 ggcgtcaggc cgcaccagat atccacctgc cccagcaaag ggggctccgg ttgcacggtg
     3601 agcattcgcc ggatgccagg agcggccgcc tttatccgct tcattccgtc cacgacgaac
     3661 tcgaagtctt tgggcgccgg ctcatcaaac caataggtga atgccttggg cagccagttc
     3721 cgtt
//
LOCUS       DATCGS010000542         4925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_95707, whole genome shotgun sequence.
ACCESSION   DATCGS010000542 DATCGS010000000
VERSION     DATCGS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95707"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VIL39_11100"
     CDS             complement(<1..551)
                     /locus_tag="VIL39_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY5482020.1"
                     /translation="MTSPDTEFVSILGAAGSPEAPARVSSSSLPGLMPILGLSDIVIF
                     PGMVAPLLVETAQSIKLIDDVVGGDRLLGVVLQRNAEAENPLPKEMHEVGCAARVLKM
                     LKFPDNTVRVLVEGLWRIQVKEYPKTEPYLSAKFELLKDLKEDSVELTAMMRNAQSQF
                     QEVIKLSPALSEQIKIAALNTEDP"
     gene            complement(614..955)
                     /locus_tag="VIL39_11105"
     CDS             complement(614..955)
                     /locus_tag="VIL39_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY5482021.1"
                     /translation="MPNTDVYATDNGLVVKVELAGMRSEHLEITVEGSRMRIAGNRPD
                     GCRSAKASFLVMEITYGPFESVLELPAGYDLSQAKAAYLNGFLRIDVPLAQRQTKNTR
                     VLVAEGNSHLP"
     gene            complement(1093..1299)
                     /locus_tag="VIL39_11110"
     CDS             complement(1093..1299)
                     /locus_tag="VIL39_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482022.1"
                     /translation="MDWQQMVSLLVVASAAVLLAGSKFRRWKFSFEWDTHCGGAAVQE
                     ASLQSSIVFRARNRQRAEVLVKMR"
     gene            complement(1408..2694)
                     /locus_tag="VIL39_11115"
     CDS             complement(1408..2694)
                     /locus_tag="VIL39_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5482023.1"
                     /translation="MTASLDSRLSTLDSQLLLRARVVLPVSQPPIREGAVLVSGKRIA
                     AVGRWRDLCAHSRNKTVDLGEAVLMPGLVNAHCHLDYTDMAGQFPPPKVFADWLKLIT
                     EAKAGWSYSDYAESWLNGARMLVRTGTTSVADIEAVPELLPKAWGETPLRVFSFLEMI
                     GIKGYRQPSAIVRDAVERIDALKGFRGRPGLSPHAPYSTLPELMRLSAQTARRRKWRL
                     TTHLAESAMEFEMFARGRGEMFDWFQRSGRDMSDCGLGSPVQHLERCGALSERLLAAH
                     ANYLDKQDAVLLAKRKVHVVHCPRCHFYFHHDPFPLRRLAKAGVNVCLGTDSLASVYK
                     ARRQKVELSLFEEMRALAKNEPSLSARKIVQMATLNGARALGMGGQIGELAQGSLADL
                     VALPFAGKIADIYDGVLHHQGDVAASMIDGRWAIAL"
     gene            complement(2722..3069)
                     /gene="raiA"
                     /locus_tag="VIL39_11120"
     CDS             complement(2722..3069)
                     /gene="raiA"
                     /locus_tag="VIL39_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5482024.1"
                     /translation="MILSTHNLTLTKAIEDHIISRITKLEHLDRFAIDARVTLEHDKT
                     KAPERQFSCSIRLSVPGPDLFAEDVESDLYAAIDLVAKKIEQQIRKRHRRIKARKHKQ
                     AVRGKSSRQDKGQ"
     gene            3201..3980
                     /locus_tag="VIL39_11125"
     CDS             3201..3980
                     /locus_tag="VIL39_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5482025.1"
                     /translation="MPTKVIAVANQKGGVGKTTTAVNLAACVAAVGRRVLLFDLDPQA
                     NATSGVGLEKKAGASAYLALLGQGGLLDKIQKTAFERLHAIPSEVDLCGADIELARSD
                     NHLQRLTQALQPVLESQRFDVILVDCPPSLGILTLNAFVAAHGVLVPLQCEYYALEGI
                     SMINRIMTQLHDNGANPRLELTGVVMTMYDGRTKLSQQVVSEVREHFGEQVFETMIPR
                     NTRLAEAPSFGKPILHYDKYSSGSAAYELLAQEFLARLDKG"
     gene            complement(4107..>4925)
                     /locus_tag="VIL39_11130"
     CDS             complement(4107..>4925)
                     /locus_tag="VIL39_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY5482026.1"
                     /translation="PEGAQRNRLMEVVGNSDALSEYIHTRDYVDILKQFHEARFESPA
                     AFLAQLSPVVPRLYSIASSLQAHPNEAHLCITVVRYETHGRAKKGLASGFFADHSDMF
                     VKNIPVYVQESRSFRLPKDGSRDIIMCGPGVGLAPFRAFLEQRVLDGATGRNWLFFGE
                     QHQATDFLYGNELLDYQRNGKLHQLDLAFSRDQSHKLYVQHRMLEQAKELWSWLQNGA
                     YFYVCGDARHMVKDVHQALIAIAQMQGGLSPEAAAEYVNVTLMKTEKRYLRDVY"
BASE COUNT         1011 a         1550 c         1379 g          985 t
ORIGIN      
        1 ggatcctccg tgttgagcgc cgcaatcttg atctgctcgg acagcgccgg cgaaagcttg
       61 atgacctctt ggaactggct ctgggcgttg cgcatcatcg ccgtcagttc caccgaatct
      121 tccttcaggt ctttcagcag ctcgaacttg gcggacagat acggctcagt cttgggatac
      181 tccttgacct gaattcgcca gaggccctcc accagcacgc gcacggtgtt gtccggaaac
      241 ttgagcatct tcaacacgcg cgccgcgcag ccgacctcgt gcatctcttt tggcagcggg
      301 ttctccgcct ccgcattgcg ctgcaaaacg accccgagca ggcgatctcc gcccacgacg
      361 tcgtcaatga gttttatgct ttgcgccgtc tcgaccagca gcggcgccac cattccggga
      421 aagatcacga tgtctgacaa gcccagaatg ggcattagcc ccggcaagga gctggaagaa
      481 acacgcgccg gcgcctccgg actcccggca gctcccagga tactgacaaa ttcggtgtcg
      541 ggcgaggtca tggtcattcc acggtcatgg tcgctccata ctgcagttta gatgccgccg
      601 ccccgaaaaa agttcaggga agatgtgaat tcccttcggc aacaagcacc ctggtgtttt
      661 ttgtctgccg ctgtgccagc ggcacatcaa tccgcaagaa tccattcagg taagccgctt
      721 tggcctggct caaatcgtag cccgccggca gctccagcac gctctcgaat ggcccgtatg
      781 tgatctccat taccaggaag ctggccttag cggaccggca tccatccggc cggttgcccg
      841 cgatccgcat tcggctcccc tccactgtaa tctccaagtg ttcgctccgc atcccggcca
      901 gctctacctt caccaccagc ccattgtccg tcgcgtaaac gtccgtgttg ggcacccaat
      961 gggccctcgc tgaaatgtcc tgctccccag tggcagagcg cgttataaaa tgcacggagg
     1021 aactgacttt gcacatggcc atcttcagcg aaaaagtttt aactcagccg ttgcggctgc
     1081 gcaagctccc tttcatctca ttttcaccag cacttcggca cgctgcctat tgcgagcgcg
     1141 aaacacaatc gaactctgca gagaagcctc ctgcacggca gcaccgccac aatgcgtgtc
     1201 ccactcgaaa ctaaacttcc accggcggaa cttgctcccc gccagcaaca ccgcggcgga
     1261 ggcaaccacc aataacgaca ccatctgctg ccaatccatg ctagcaacat aacctcaaac
     1321 aggggttgcg caacaaatga ttctgtgcag cacatccata ttccttgtca ttcatttagc
     1381 aaaatgtgca aaccaatccc gacgtcatca gagtgcgata gcccatcggc cgtcaatcat
     1441 gctggctgcc acatcaccct gatgatgcag cacgccgtcg tagatgtccg ctattttccc
     1501 ggcgaaagga agcgcgacga ggtcggcaag agaaccctgg gcaagctcgc cgatctgccc
     1561 gcccatgccc agggcgcggg cgccgttgag cgtagccatc tgcacaatct ttctggccga
     1621 aagggacggc tcattcttcg cgagcgctcg catttcctcg aacaggctca actccacctt
     1681 ttgacgccga gccttgtaaa cgctcgccag gctgtcggtg ccgaggcaaa cgttgactcc
     1741 cgctttcgcc agccgccgca gcggaaacgg atcgtggtgg aaatagaagt ggcagcgcgg
     1801 acaatgaacc acatgcacct tgcgcttcgc caacaagact gcatcctgct tatcgaggta
     1861 gttcgcgtgc gcggccaaca gcctctcgct cagcgcgccg cagcgttcca ggtgctgaac
     1921 cggcgaaccc aggccgcaat ccgacatgtc ccgcccgctg cgttggaacc agtcgaacat
     1981 ctcgccccgc ccgcgcgcaa acatctcgaa ctccatcgcc gattcggcca ggtgcgtggt
     2041 cagtcgccac ttgcgtcggc gcgcggtctg agcgctcaat cgcatcagct ccggcagggt
     2101 tgaataggga gcatgcggcg agaggcccgg gcgaccgcgg aagcccttga gcgcatcaat
     2161 tcgctccacg gcatcccgaa caatggcgct gggttggcga tagcccttga tgccgatcat
     2221 ttccaggaac gagaacacgc gcagcggcgt ctctccccag gctttcggca gcagttccgg
     2281 cacggcttcg atgtcggcca cgctggtggt gccggtacgg acgagcatcc gggcgccgtt
     2341 cagccaggac tcggcatagt cggagtagct ccacccggct ttcgcctcgg tgatcagctt
     2401 aagccaatcc gcgaacactt tcggcggcgg gaactggcct gccatgtcgg tgtaatccag
     2461 gtggcagtgc gcattcacga ggccgggcat taacactgct tcgcccagat ctacggtctt
     2521 gttacggctg tgcgcgcaca aatcgcgcca ccgcccgaca gcggcgatgc gtttgcccga
     2581 aaccaagact gcgccctcgc gaatcggcgg ttgggacacc ggcagaacga cgcgggcgcg
     2641 caggagaagt tgagagtcga gagtcgagag tcgagagtcg agtgacgcgg tcatctcact
     2701 atcaagcatc aaccagtccg cttactgtcc cttgtcctga cggctgcttt tgccgcgcac
     2761 ggcctgtttg tgcttgcggg ctttaattct cctatgccgc ttacggattt gctgctcgat
     2821 cttcttggcg accaggtcga tggcggcata gagatcgctt tcaacatctt cagcaaacag
     2881 atcggggccg ggcaccgaca gacgtatgga gcaggagaat tgccgctccg gcgctttggt
     2941 cttgtcgtgc tccagcgtta cgcgcgcatc aatggcaaag cggtccaagt gctccagttt
     3001 agtaattcgg ctgataatgt gatcttcgat ggctttggtc aaagtcaggt tatgagtcga
     3061 aaggatcatg ttcatttaga tatcatgcag ccagccggga acaaaagcta gcgaaagtgt
     3121 gctccctggg cgaaagagga aatctgacct catcgccgcg aaaacttgaa tcggcgcctc
     3181 gattccggta ttcctacccc gtgccaacca aagtcatcgc tgtcgccaac cagaaaggcg
     3241 gagtcggcaa gacgaccacg gcagtcaacc tggctgcctg tgtcgcggct gtcggccggc
     3301 gcgtcctgct gttcgacctc gatccgcagg ccaacgcgac cagcggcgtc ggcctggaaa
     3361 agaaagcggg cgccagcgcc tacctcgcgc tgctgggcca gggcggcttg ctggataaga
     3421 ttcaaaagac cgcctttgag cgcctgcacg ctattcccag cgaagttgat ctgtgcggtg
     3481 cggacatcga gttggcgcgg tcggataatc atctgcagcg cctcactcaa gcgttgcagc
     3541 ccgtgctgga atcccagcgg ttcgacgtga ttcttgtgga ttgcccgcct tccctcggca
     3601 ttctcacgct caacgcgttc gtggccgccc acggggtgct ggtgcccttg caatgcgagt
     3661 attacgccct cgaaggcatt tcgatgatca accggatcat gacccagttg catgacaacg
     3721 gcgccaaccc caggctggag ctgacgggag tggtgatgac catgtatgac ggccggacca
     3781 agctctcgca gcaagtcgtc agcgaggtgc gcgagcactt tggcgagcag gtgttcgaga
     3841 ccatgattcc ccgcaacaca cgcctggccg aggcgccgag cttcggcaaa cccatcctcc
     3901 actacgataa atacagcagc ggctccgccg cctacgaact gctcgcacaa gagttcctgg
     3961 cgcggttgga caagggctga gaagttgcag ggtgacaagg ttacaaagtt acatgggtta
     4021 caccggttac atgggttaca cgggagccgc gatgtaactg atgtaactca tgtaactaat
     4081 gtaacccatt caacttatgt aactactcaa tacacatccc gcagataccg tttctccgtc
     4141 ttcatcagtg tgacgtttac gtactccgcc gccgcttcgg gggaaagccc tccctgcatc
     4201 tgagcgatgg caattagagc ttggtgcacg tccttcacca tgtggcgcgc gtcgccgcat
     4261 acgtaaaagt aagccccatt ctgcagccac gaccacagct ctttcgcctg ttcgagcatg
     4321 cgatgctgca cgtagagctt gtgagactgg tcgcgggaga aggccaagtc gagctggtgc
     4381 agcttgccgt tgcgctggta atccagcagt tcgttgccat aaaggaaatc cgtcgcctga
     4441 tgctgttccc cgaagaacag ccagttccgc cccgtcgcgc catcgagcac gcgctgctcc
     4501 agaaacgcgc ggaacggcgc gaggcccacg cccgggccgc acatgatgat gtccctcgaa
     4561 ccgtccttcg gcagacggaa gctgcgcgac tcctgcacat agaccgggat gttcttcacg
     4621 aacatgtccg agtggtcggc gaagaagccc gaggccaatc ccttcttggc gcggccgtga
     4681 gtttcatagc gcaccacggt gatgcacaga tgcgcctcgt tggggtgcgc ctgcagcgag
     4741 gaggcaatcg aatagagccg cggcaccacc ggcgacagtt gtgccaggaa tgccgcgggt
     4801 gactcgaacc gcgcctcgtg gaactgcttc aggatatcca cgtagtcgcg cgtatgaatg
     4861 tattcgctga gagcgtcgct gttgccgacg acttccatca accggttgcg ctgcgcgcct
     4921 tccgg
//
LOCUS       DATCGS010000543         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_95726, whole genome shotgun sequence.
ACCESSION   DATCGS010000543 DATCGS010000000
VERSION     DATCGS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95726"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VIL39_11135"
     CDS             complement(<1..461)
                     /locus_tag="VIL39_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5482027.1"
                     /translation="MSNEPSVIEVTNLRHKYGHTEAVNDLSLRVQRGQCYGFFGRNGA
                     GKTTTIKCLLNLLRPMSGTVRVFGLDPAREEVAVKSRLAYVPDYVAFYPWMTVRNVLD
                     YLASFRPGWNRKTERELLGQFRLDDAKKASALSRGQRTQLALMGAICAEPEL"
     gene            complement(464..877)
                     /locus_tag="VIL39_11140"
     CDS             complement(464..877)
                     /locus_tag="VIL39_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5482028.1"
                     /translation="MLIQLSFKSGKPVYLQVVDQVKAAAASGALRTGEPLPSIRPLAE
                     QLRVNRNTVAKAYTELEGQGVIETIAGRGCFLKENHSPFRKDIRLKMVAEEIDAAIVQ
                     AHHLRVEDKEFLDLVKERLEVFVQTNKAHEAKAKE"
     gene            1459..3246
                     /gene="dnaG"
                     /locus_tag="VIL39_11145"
     CDS             1459..3246
                     /gene="dnaG"
                     /locus_tag="VIL39_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5482029.1"
                     /translation="MAGLFSSATLEQIRAASDIVDVIGSYVPLKRAGANFVAVCPFHK
                     EKTPSFNVNPQRQIFHCFGCHKGGDVFAFVKEYENIDFPEAVRRLADRAKIPLEFEKN
                     AGEQQSRHIKERLLQVHEQIAQRWQGALANEASGQIARDYLARRGVSAEAVKLFRLGY
                     APDLWDDTVNWAKSKGHELALVEQAGLILRKREGDGYYDRFRGRLMFPICDEQGRVIG
                     FSGRILTGDEKTAKYVNSPETPIFTKSKVFFGLDKSKRAVLEAGYAIVCEGQLDLIAC
                     FMAGVQNVVAPQGTAFTADHARILKRYVEEVVLCFDSDEAGQNATVRSLDNLLASGLA
                     VRVAVVSAPHDPDSFIKASGGPAFKQLIEQAEGFFDYYLNRLCATNEVTTDKGRLAVL
                     RGMAEAVHKTGSSVLVDKYAQKTALRLGVTPDAVRAEFRKLSRAKAPAPEAVEPPVEE
                     SNSPQRPSTPEYWLLKLLLSHDDLVVWAAAHVDPGWVQHSLVRQIVAQRLAAQANQTW
                     TSLAAFLDECEVSQMRSLITEATAEPRPIPNPSQQLSDVALRLRNQFLDRQLAALMQS
                     ANQPECSDAERLDLLHQQQELRRLKRQPI"
     gene            3359..3706
                     /locus_tag="VIL39_11150"
     CDS             3359..3706
                     /locus_tag="VIL39_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482030.1"
                     /translation="MKTSRWIHSAIMALTLAMSGCSKQGSVDTALFEKSFKTAEAAVQ
                     TGADKVVAAIKSADYSGALAELKTLANNAKLTPDQQQAIKGVMAQVEKLIADAATKRG
                     KPRVETRGTIEHQ"
BASE COUNT          757 a         1105 c         1121 g          736 t
ORIGIN      
        1 agctccggct cggcgcagat tgctcccatg agcgccaact gcgtcctctg gccgcgggac
       61 agcgcgctcg ccttcttggc gtcgtcgagc cggaattgcc cgagtagctc gcgctccgtt
      121 ttgcggttcc agccgggccg aaaggaggcc aggtagtcca gcacgttgcg cacggtcatc
      181 catgggtaga acgccacata atccggcaca tacgctagcc gggacttgac cgcgacctcc
      241 tccctggccg ggtccaggcc aaagacccgg accgtccccg acatgggccg cagcaggttc
      301 agcaggcatt tgatggtggt ggtcttccca gcgccgttgc ggccgaaaaa gccgtaacac
      361 tggccgcgct gcacccgcag gctcaggtcg ttgacggctt cggtgtggcc atacttgtgg
      421 cgcagattgg tgacttcaat gacggagggt tcgttgctca taattattcc ttcgctttgg
      481 cctcatgggc cttgttggtt tgtacaaata cttccagccg ttccttgacg aggtctaaga
      541 attccttgtc ctccacccgc aaatggtggg cctgtacaat ggcggcatca atctcctcgg
      601 ccaccatctt gagccggatg tccttccgga acggggaatg gttctccttc agaaagcagc
      661 cccgcccggc gatcgtttcg atcaccccct ggccctcgag ctccgtgtac gccttggcca
      721 ccgtgttccg attgacccgc aactgctccg ccaatggccg gatcgaaggc agcggctcgc
      781 ccgtccgcaa cgcccccgag gccgccgccg ccttcacctg gtccaccact tgcaagtaaa
      841 ccggcttgcc ggatttaaaa ctgagctgaa tcaacatact ttggcctgct gttatatact
      901 gtcataggac aataggacag tgtgccacgc ctgtcaacaa cctttttacg attattttgg
      961 cgggcgcagt tggcaccacg aagtacacca aatacacgaa atacagaagt cgtgccagga
     1021 gacgacggct cggccgtccg tcgaatgcct gggtggttct gcggtggatt cggtggaatt
     1081 aggcttccgg ccagctccgt taggagcgcc ttgtttatag caacgagtac cccccgctgt
     1141 ccggccaagc tccgtaggag cggcatggtt tgagttgaat gcgcggctgc gaaagggttt
     1201 gcagcgtgca cctctatgcc gctcctgccg gagcttgttt caatcaagga cgccttttac
     1261 tatgaacatg gcgctcctaa cggagctgtc tccccacgcc ggcaccctgg tccaccggag
     1321 aaagcagaaa agcatccagg ctacgccagt cctaggtcat ttcgtgtagt tggtgtattt
     1381 cgtggttccc ctctccgttc ctgaaatgat tttaaggctc ggcaccaggg ggcgtccgtc
     1441 atattctcgc ttaagactat ggccggcttg ttttcttcgg cgacccttga gcaaatccgc
     1501 gctgccagcg acatcgtgga tgtgatcggg tcgtatgtgc cgctcaagcg cgccggggcc
     1561 aacttcgtcg cggtctgccc gtttcacaaa gagaagacgc ctagcttcaa cgtcaacccc
     1621 caacgccaga tattccattg ctttggctgc cataagggcg gcgatgtgtt tgcgttcgtc
     1681 aaggaatacg agaacattga tttcccggag gccgtgcggc ggctggccga ccgggcgaag
     1741 atcccactgg aattcgagaa gaacgccggc gagcagcagt cgcggcatat caaggaacgg
     1801 ctgctgcaag tgcatgagca aatcgcgcag cgttggcaag gcgccctggc taatgaagcc
     1861 tccgggcaga ttgctcgcga ctacctggcc cgccgcggcg tgtctgcgga ggcggtgaaa
     1921 ctgttccgcc tcggctatgc gccggacctg tgggacgaca cggtgaactg ggccaaaagc
     1981 aaaggccacg aactggcgct ggtcgaacag gcggggctga tcttgcgcaa gcgggagggg
     2041 gacggctact acgaccgctt ccgcggaagg ctgatgttcc cgatctgcga cgagcagggg
     2101 cgggttattg gtttcagcgg gcggattctg actggggatg agaagacggc caaatatgtc
     2161 aactccccgg agacaccgat ctttaccaag agcaaagtgt tcttcgggct cgacaaatcc
     2221 aagcgcgcgg tgctggaggc ggggtatgcc atcgtgtgtg aagggcagct cgacctgatc
     2281 gcctgcttca tggctggggt gcagaacgtc gttgcacccc agggcaccgc tttcaccgcc
     2341 gaccacgccc gcattctcaa gcgttacgtc gaggaggtcg tgctttgctt cgactcggac
     2401 gaggccggac agaatgcgac ggtccggtcg ctcgataatt tgctcgcgtc cgggctggct
     2461 gtacgggtcg ccgtggtgtc cgcgccccat gacccggaca gcttcatcaa ggcttcgggc
     2521 ggccccgcct tcaagcagct tatcgagcag gcggaaggct tcttcgatta ttacctcaac
     2581 cgcctgtgcg ccaccaatga agtgacgacg gacaaaggcc gtctggccgt cttgcgcggc
     2641 atggccgaag ccgtgcacaa gaccggcagc agcgtactgg ttgataaata cgcgcaaaag
     2701 accgctttgc gcctgggcgt aacgcccgac gccgtgcgcg cggagttcag gaagctgtcg
     2761 cgggcaaagg ctccggcgcc cgaagccgtg gagccgccgg tggaggaaag caacagcccg
     2821 caacggccat caactcccga atactggcta ttgaagctgc tgctgtcgca cgacgacctg
     2881 gttgtctggg ccgccgcgca tgttgatccg ggctgggtac agcattcgct ggtaaggcaa
     2941 atcgtggccc agcgcctggc ggcgcaggcg aatcaaacct ggaccagcct ggccgccttt
     3001 ctcgatgagt gtgaggtttc ccaaatgcgg agcctcatta cggaagctac ggctgagcct
     3061 cgcccgatac ccaatccgtc gcagcaactc tctgatgtcg cgttgcgcct tcggaatcag
     3121 tttctcgatc gtcagttggc cgcgttgatg cagagcgcca accagccgga atgcagcgac
     3181 gccgaacggc tcgacttgtt gcaccagcaa caggagttgc gacgccttaa gcgtcagccg
     3241 atatagacgc gcagaggttc gaggttgacc ttccctgagc ctgcaggtat ggtgaaacca
     3301 ggttagccgg ttgtatgtga tagcccagga aaccagtcga acaacgaaag agcattgaat
     3361 gaaaacctcc agatggatac acagcgccat tatggctctg acgctggcga tgtccggctg
     3421 cagcaagcag ggcagtgttg ataccgcatt gttcgagaag agcttcaaaa cggctgaggc
     3481 cgccgtgcag actggcgccg acaaagtagt ggctgccatc aagtccgccg attactccgg
     3541 cgcgctggcg gaattaaaga ccctcgccaa taacgccaag ctgacgcccg accagcagca
     3601 ggccatcaag ggtgtgatgg cccaggttga gaaactcatc gctgatgctg ccaccaaacg
     3661 tgggaagcca agggtggaaa cacggggaac aatagagcac caatagagga acaatagag
//
LOCUS       DATCGS010000544         3856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_145955, whole genome shotgun sequence.
ACCESSION   DATCGS010000544 DATCGS010000000
VERSION     DATCGS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145955"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIL39_11155"
     CDS             complement(<1..671)
                     /locus_tag="VIL39_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482031.1"
                     /translation="MRTALKIVLAGALVAISAGVPCVLQHQAQLELGARRAAFEQQAS
                     RLSLLVAENGRLSNLVAQAKVPPPLAAEQLRELLRLRNEKRELAEQTNRLARLVAGTS
                     DQAQPSEAGPETALCAEMTEALKVILPALQPALQKYALAHPNQPPDSFAELQDYFPLV
                     AGRKMAGFQTFEFAREDGPRPGDALLLRGNAGRRLTDGNEVRVYGFSDGHVVEVASED
                     GRFDA"
     gene            complement(668..2089)
                     /locus_tag="VIL39_11160"
     CDS             complement(668..2089)
                     /locus_tag="VIL39_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5482032.1"
                     /translation="MTDNQTLVVEYVRTGSEPAFRELVARYVDLVYSSALRLVGGDAQ
                     LAEDVSQTVFLNLARKAHRLPQEVMLGGWLHRDTCYAARTLMRSERRRQQRERQAMEM
                     SALEDHSQANLATLAPLLDEAIEDLGAEDRTAILLRFFEQRDFRAIGHALGSNEDAAR
                     KRVTRAVEKLHGLLKVRGVSLSAAALGTVLATGTITAAPAGLAANLAGTALAGAVAGA
                     GFSATLIKLATMTKIQAGVVGAVVLIGAAATVVVQHQAHAAFRAQDESLRQQSAELSR
                     QQTENERLAGLVQTSGSRANTLDDLVSLRNEVDSLRKQTNSLAASLEEKRRLQARTAS
                     PSAGSTATLEAMEEDRRLMVAGMNYTKQWLLAFLLFSSENHDSFPGSFDEAQSFLPKQ
                     AAAETNFTTGQFEILYQGPITNITSPALTVVMRQKQPRHNSSGRWSRTYGFADGHCEV
                     ASTPDGNYDAWEKQHIVAPSPDR"
     gene            complement(2237..>3856)
                     /locus_tag="VIL39_11165"
     CDS             complement(2237..>3856)
                     /locus_tag="VIL39_11165"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY5482033.1"
                     /translation="GGSASLDPRHITQLLVFVSRPSADHLFELQDIRATGAYQPPTAW
                     ITDADPFFPFINTFGQYRHKDWPGKVKSVADLKAGREAEAKDLARLPGPKDWDKFGGW
                     AAGPQLQATGFFRTEKVEGKWWLVDPDGRLFFSHGIDCVRMLDATPIDERASWFADFP
                     GNQPEFAAFRSRGSALKGHYAGCTPECFSFAGANLLRKYGSDWREAYPRLIHQRLRSW
                     GLNTIANWSDGATCRLRLTPYTDTVGSGRAKPIEGSEGYWGKFPDVFDPGFADGLRRS
                     TEGKRNDSAGDPWCIGYFSDNEMSWGDDTSLALGTLQSPPEQVAKQMFVADLKAKYAD
                     IERLNQTWGTHHTSWDALLRSRQAPDKTKANDDLAAFYTHTAEQYFRTVREVIKAVAP
                     NQLYLGCRFASVNARAAAAAARYCDVVSYNLYHPNVADFKFNGGADVPLIIGEFHFGA
                     LDRGLFHTGLVPVATQQARAEAYKEYVRSALRHPQFVGCHWFQYCDEPTTGRVWDEEN
                     YQIGFVDIADTPYAETIAASRAVGDELYGKR"
BASE COUNT          670 a         1232 c         1217 g          737 t
ORIGIN      
        1 caggcgtcaa agcgaccgtc ctcggaggcc acctccacga cgtgcccgtc actgaacccg
       61 taaacccgta cctcattgcc atccgtgagc cggcggcctg catttccacg caaaagcaag
      121 gcgtcgccgg gtctcgggcc gtcttcccga gcgaattcaa aggtctggaa tccggccatc
      181 ttccgccctg caaccagggg aaaatagtct tgtagctcgg cgaagctgtc ggggggctgg
      241 ttggggtgag ccaaagcata cttttgcagg gcgggctgga gggcgggcag gatgactttc
      301 agggcttcag tcatttcagc gcacaacgct gtttcaggcc cggcctccga gggttgggcc
      361 tgatccgacg ttccggcgac caaccgcgcc aaccggttcg tctgttcggc cagctcacgt
      421 ttctcgttgc gcagccgcaa cagctcgcga agctgctccg cggcgagcgg cggtgggacc
      481 tttgcctggg cgacgagatt ggaaagcctc ccattctccg caaccaggag cgacaaccgg
      541 ctggcctgtt gctcgaaggc cgctcgcctg gcgcccagct caagctgggc ctggtgttgc
      601 aggacgcacg gcactcctgc gctaatggcg acgagcgcgc cggcgaggac gattttgagc
      661 gcggttctca tcggtccggc gacggggcaa cgatgtgctg cttttcccag gcatcataat
      721 ttccatcagg ggtagaggcg acttcgcaat gcccatctgc aaagccatag gtccgcgacc
      781 atctgccgct ggagttgtgg cgtggctgct tttgccgcat gacgacggtc agggccgggg
      841 aggtgatatt cgtgattgga ccctgataca gaatctcgaa ctgcccggtg gtgaagttgg
      901 tttcggccgc ggcttgcttc ggcagaaacg actgcgcctc gtcgaagctg cccggaaaac
      961 tgtcatggtt ctcgctggaa aacagaagga acgccaggag ccattgcttg gtgtaattca
     1021 tcccggctac catcagcctc ctatcctcct ccatcgcctc cagggttgcg gtggacccgg
     1081 ccgatgggga tgccgtgcgg gcttgcaacc ggcgcttttc ctccaggctg gcggcgaggc
     1141 tgttggtctg cttccggagc gagtccacct cattgcggag gctcaccagg tcgtcgaggg
     1201 tgttggcgcg cgagccgctg gtctgcacca aacccgccag ccgctcgttc tcggtctgtt
     1261 gccgggacag ctctgccgac tgctgccgca gcgactcgtc ttgcgcccgg aacgcggcat
     1321 gcgcctgatg ttgaaccacc acagtcgccg ccgccccaat caggaccacg gccccgacca
     1381 cacccgcctg gatctttgtc atagtagcta gtttgatgag agtggctgag aagccagcgc
     1441 cggcaaccgc gcctgcaagg gcggtgcccg cgaggttcgc cgccagccca gccggcgccg
     1501 ccgtgatggt tcccgtcgcc agcaccgttc ccagcgccgc cgcggacagg ctcacgcccc
     1561 ggactttgag cagcccatgt agtttctcca ccgcgcgcgt cacccgcttc cgcgcggcat
     1621 cctcattgct gcccagcgca tggccaatgg cacggaagtc acgctgctcg aagaaccgga
     1681 gcaggatggc ggtgcgatcc tccgccccaa ggtcctcgat cgcctcgtct aagagcggtg
     1741 ccagtgttgc caggttagcc tgcgagtgat cttccagggc gctcatttcc atcgcctgcc
     1801 tttcacgttg ctgacgacgg cgctcgctgc gcatgagcgt gcgggccgcg tagcacgtat
     1861 cccggtgcag ccagccaccc agcatcacct cctgaggcag ccgatgagcc ttgcgtgcca
     1921 ggttgagaaa cacggtctgg ctcacatcct ccgcgagctg cgcgtccccg cccaccagcc
     1981 gcaatgccga cgagtaaacc aagtccacgt accgcgccac cagctcccga aaggccggct
     2041 ccgagcccgt cctcacgtat tccaccacca atgtctggtt gtccgtcatc ttcaatctac
     2101 tagtaaaacg ccgctcagaa cccaaaccgg acaatcgctt ctcagaaatc cttgccgtct
     2161 ccagccggtc ggaggctgct gcgcccgggc gcgctccggc ccagttgccg ttgctgatat
     2221 attgcaccct tcccgttcac cgcttcccgt acagctcatc ccccaccgcg cggctggcgg
     2281 cgatggtttc cgcataggga gtatcggcga tatcaacaaa gccaatctgg tagttctctt
     2341 catcccacac gcgcccggtg gtcggttcgt cgcagtattg aaaccaatgg caaccgacaa
     2401 actgcgggtg gcgtagcgcg ctcctcacgt attccttgta ggcctccgcg cgcgcctgct
     2461 gggtggccac cggcaccagg ccggtatgga acaggccgcg gtccagcgct ccgaagtgga
     2521 attctccgat gatcaagggc acgtcggcgc cgccgttgaa tttgaaatcc gcgacgttgg
     2581 ggtgatagag attgtaactg acgacgtcgc aatatctggc cgcggcggcg gcggcgcggg
     2641 cattgaccga ggcaaagcgg cagccgaggt agagttggtt tggcgcgacg gctttgatga
     2701 cctcgcgcac cgtgcggaag tactgctcgg cggtgtgggt atagaaggcg gccaggtcgt
     2761 cgttggcttt ggttttatca ggcgcttggc ggctccggag cagggcatcc caggaggtgt
     2821 gatgggtgcc ccaggtctga ttcagtcttt ctatgtcggc gtatttggcc ttgaggtcgg
     2881 cgacaaacat ctgcttggca acctgctcgg gcggggactg cagcgtgcca agcgccaggg
     2941 aggtgtcgtc accccatgac atttcgttgt cggagaagta gccaatgcac caggggtcgc
     3001 cggcgctgtc gttccgcttg ccttccgtag agcggcgcag cccgtcagca aagcccggat
     3061 cgaacacatc ggggaacttg ccccagtagc cttcgctgcc ttcgattggt ttggcgcggc
     3121 cggagcctac ggtgtctgtg tagggtgtga gtcgcagccg acaggtggcg ccatcggacc
     3181 aattggcgat ggtgttgagc ccccaactgc gcaggcgttg atggatgagc cgcggatagg
     3241 cctcgcgcca atccgagccg tatttccgca gcaggttggc cccggcaaag gagaaacact
     3301 ccggcgtgca ccccgcataa tggcctttga gggcggagcc gcgcgagcgg aacgcggcga
     3361 actccggctg attgccgggg aagtccgcaa accagcttgc ccgctcgtca atcggagtgg
     3421 cgtccagcat ccgcacgcaa tcaatgccgt gcgagaaaaa cagccggccg tccgggtcca
     3481 ccagccacca cttgccctcc actttttccg tgcggaaaaa gcccgtcgct tgcaactgcg
     3541 gacctgcggc ccagccgcca aacttatccc aatccttggg gccgggcagg cgggcaaggt
     3601 ctttggcttc cgcctcgcga ccggctttga ggtcggcgac cgatttgacc ttgccgggcc
     3661 agtctttgtg acggtactgg ccgaaggtgt tgatgaaggg aaagaacggg tcggcatccg
     3721 taatccacgc ggtcggtggc tggtaggcgc cggtcgcgcg aatgtcctga agttcaaaaa
     3781 ggtggtcggc gctgggccgg ctgacgaaga cgagcagttg ggtgatgtga cgcggatcga
     3841 ggctggccga accgcc
//
LOCUS       DATCGS010000545         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_640623, whole genome shotgun sequence.
ACCESSION   DATCGS010000545 DATCGS010000000
VERSION     DATCGS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640623"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VIL39_11170"
     CDS             complement(<1..1073)
                     /locus_tag="VIL39_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY5482034.1"
                     /translation="MRAQASKSQEKTALAIVRQLQGAGFLAFWVGGCVRDFLLGREPG
                     DYDIVTSALPEQIEGLFKRTIPVGRKFGVMVVIEDGHQFQVATFRAEADYQDGRRPER
                     VVFGDAEADVRRRDFTVNGLFYDPVRKESHDWVGGEADLRARIIRTIGAPEERFAEDH
                     LRLLRAVRFAAQLDFSIEAGTFAALQANAAKIKTISAERVREELVKLLRPPHASRGLD
                     LLRESGLLEQVLPEIAATVACEQSPDFHPEGTVFNHLRLMLQHLPPEADPSLPWAVLL
                     HDAAKPVTASADPETGSRHFYGHEKIGADMAAEILERLRFPRKQMNEVVEAVRHHMQF
                     KDTLQMRKSTLRRLLMRPTFPLEL"
     gene            complement(1111..2025)
                     /locus_tag="VIL39_11175"
     CDS             complement(1111..2025)
                     /locus_tag="VIL39_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5482035.1"
                     /translation="MYSCSTCWNSHRHTDGRAMLREIRELGFQYAELGHGTRISLLPG
                     ILEAVDAGEMKISSLHNFCPLPIGVNNAAPNLYQFSSERARERELAERYTLKTIEFAV
                     RVKASVVVLHCGSIDMKDYTDKLLDLAGQGEKAAVKFAKLCSEADEKREAKKGPFVER
                     VNELLKKLLPVAEGQGIKLGIENRQALEELPVETDFHFLFRELNSPNLVYWHDTGHAQ
                     IKENLKFIHHAMHLESLLDHLYGLHVHDVQFPGRDHCAPGTGMIDFAALKPLIKPEHI
                     KVFELSPSLTVDAVKGGIEHLKRIWGEE"
     gene            2151..2495
                     /locus_tag="VIL39_11180"
     CDS             2151..2495
                     /locus_tag="VIL39_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482036.1"
                     /translation="MPLKLVNPQLPGNCPGNSLAFSPASSTLLAMPLPSQPVTLTVKQ
                     IAALNKQLSSMRHDINNDLAIIVAAVELIRYKPSSAERMMAPLAEQPSKIADSLRKFS
                     AEFERNLGITRC"
     gene            complement(2492..>3249)
                     /locus_tag="VIL39_11185"
     CDS             complement(2492..>3249)
                     /locus_tag="VIL39_11185"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY5482037.1"
                     /translation="VLPAAGWLLASDFLPAIGNVVCPGAGFGYNPRRMLKALIASLKT
                     WYVGALKAGGYWVVGLMMAIESSIFPLPSEIVIPPAAYWAHTHQIPLSLTGIIIAGTI
                     GSWIGATAMYWASRLAGRPLVLRYGRYVMISQAKVEGAERWAAHYGSMGIFISRLLPV
                     VRHLIGIPAGIVRMDYRAFSLYTVLGSGVWCGVLCWVGIKAGNDEKLMKGEMHAITLW
                     LGGAMLVLGGLYYFFVHRHMKTEQKPASGAGRD"
BASE COUNT          634 a         1086 c          910 g          619 t
ORIGIN      
        1 agttccagcg ggaaggtggg gcgcatcagc aaacggcgca gcgtggactt gcgcatctgc
       61 aacgtgtcct tgaactgcat gtggtggcgc accgcctcga cgacctcgtt catctgtttg
      121 cggggaaagc ggagccgctc caggatctcc gcggccatgt ccgcgccgat cttctcgtgg
      181 ccgtagaaat gccggctgcc cgtctccggg tcggcggaag ccgtgactgg cttggcggca
      241 tcatgcagca gaaccgccca cggcaacgat gggtcggcct cgggcggcag atgttgcagc
      301 atcagccgga gatggttgaa gaccgtgccc tcgggatgga aatcgggcga ttgctcacaa
      361 gccaccgtgg ccgcgatttc cggcaacacc tgttcgagca aaccgctctc gcgcagcaaa
      421 tccagcccgc gcgacgcgtg cggcggacgc aacagcttca ccaattcctc gcgcacccgc
      481 tcggcgctga tggtcttgat cttcgccgcg ttggcttgca gggcggcgaa ggtgcctgct
      541 tcgatgctga aatccagttg cgcggcaaac cgcaccgccc gcagcagccg caggtggtcc
      601 tcggcaaagc gttcctccgg ggcgccgatg gtgcggatga ttcgggcgcg caagtccgct
      661 tcgcccccga cccaatcgtg cgactccttc cgcaccggat cgtagaacaa gccgttgacc
      721 gtgaaatcgc gccggcgcac gtccgcctcc gcgtcgccga acacgacccg ctccgggcgc
      781 cggccatcct gataatccgc ctcggcccga aaagtggcca cctggaactg gtggccgtct
      841 tcaatgacca ccattacccc aaacttgcgt ccgacgggaa tcgtgcgttt gaaaagcccc
      901 tcgatctgtt ccggcagggc cgaggtgaca atgtcataat cgcccggctc gcggcccagc
      961 aggaaatcgc gcacgcagcc ccccacccag aaggccagga acccggcccc ttgcagctgg
     1021 cggacaatgg ccagcgccgt cttctcctgg gatttggaag cctgcgctct catgtctggt
     1081 cgcttcctcc gtttgccttg cggttccgtg ctactcctcg ccccaaatcc gtttgaggtg
     1141 ttcgatgcca cccttcaccg cgtccacagt caggctgggg ctgagctcga acactttgat
     1201 gtgctccggt ttgatcagcg gcttgagcgc ggcgaagtca atcatgcccg tgcccggcgc
     1261 gcagtgatcg cggccgggaa attgcacgtc gtggacatga aggccataga ggtgatcgag
     1321 cagggactcc aaatgcatcg cgtgatggat gaacttcagg ttctccttga tttgggcgtg
     1381 gcccgtgtcg tgccaataga cgaggttcgg gctgttcagt tcgcggaaaa ggaaatggaa
     1441 atcagtctcc accggcagtt cctccagcgc ctggcggttt tcaatcccca gcttgatgcc
     1501 ctgtccttcg gccactggca acagcttctt caacagctcg ttcacccgct cgacgaacgg
     1561 ccccttcttg gcctcgcgct tctcatccgc ctcggagcaa agcttggcaa atttaaccgc
     1621 ggccttctcg ccctgcccgg ccaggtcgag cagcttgtcc gtgtaatcct tcatgtcaat
     1681 gctgccgcag tgcagcacga ccaccgacgc cttcacccgc accgcgaact caatggtctt
     1741 cagcgtgtag cgctccgcca attcacgctc ccgggcccgt tccgaggaaa actggtagag
     1801 gttgggcgcg gcgttattga cgccgatcgg cagcgggcag aagttatgca ggctggaaat
     1861 cttcatctcc ccggcgtcca ccgcttcgag gatgccgggc agcaggctga tccgcgtgcc
     1921 gtggcctagc tcggcgtact gaaagcccag ctcgcggatc tcgcgcagca tcgcccgccc
     1981 gtcggtgtgc cgatgggaat tccaacaggt agaacaggaa tacataaaac taaatcgccc
     2041 ttcttaagct taatctcggt caaaactctc tcacttcctc gccaacaact tcgggaccat
     2101 ccaggccttc atggcgggct ttcccgtcgg gtcagccggc atagcttatt ttgccgctga
     2161 aacttgtcaa tccgcaactg cccggaaact gccccggaaa ctcgcttgct ttttctcccg
     2221 cctccagcac actgttggcc atgccattgc ccagtcagcc agtcacgttg accgtcaagc
     2281 aaatcgccgc gttgaacaaa cagctctcct ccatgcgcca cgatatcaat aatgatcttg
     2341 ccatcattgt cgccgccgtg gagttgatcc gttacaaacc cagcagcgcc gagcgcatga
     2401 tggcccccct cgccgagcag ccctcgaaga ttgccgacag tctcaggaag ttctccgctg
     2461 aattcgagcg caacctcggt attacccgct gttagtcgcg ccccgcccca gacgctggtt
     2521 tctgctccgt cttcatgtgg cgatgcacga agaagtagta caagccgccc agcaccagca
     2581 tcgccccgcc cagccaaagc gtgatggcgt gcatctcgcc cttcatcagc ttctcgtcat
     2641 tgcctgcctt gatcccgacc cagcaaagca ctccgcacca gacgcccgaa cccagcacgg
     2701 tgtagaggga aaaggccctg taatccatcc gcacgatgcc ggcgggaatg ccaatcagat
     2761 ggcgaacgac cggcagcaga cgcgaaatga agatgcccat tgacccatag tgcgccgccc
     2821 agcgctcggc tccttccacc ttggcctgtg agatcatgac atatcggcca tagcgcagca
     2881 ccagtggccg gccggccagc ctcgacgccc aatacatcgc ggtcgcacca atccaggaac
     2941 cgattgtgcc ggcgataatt atcccggtca gactcaatgg aatctggtgc gtgtgcgccc
     3001 aatacgctgc cggggggatg acgatttcgc tgggcaacgg gaagatcgag ctctcaatgg
     3061 ccatcatcag gccaaccacc caatagcccc cggccttgag cgcgccgacg taccaggtct
     3121 taagtgacgc gataagtgct ttcaacatgc ggcgcgggtt atagccgaat cccgccccgg
     3181 ggcaaaccac atttccaatc gccggaagaa agtccgaagc cagaagccag ccggcagccg
     3241 gcaggacat
//
LOCUS       DATCGS010000546         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_293581, whole genome shotgun sequence.
ACCESSION   DATCGS010000546 DATCGS010000000
VERSION     DATCGS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293581"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIL39_11190"
     CDS             <1..767
                     /locus_tag="VIL39_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5482038.1"
                     /translation="KPGYLPAAGDPPNDFHWDLWLGPSPAHPWNPEYFSGGSGMNCLQ
                     WNMYWDFGSGQVGDMGSHTMDIVWNAIDAGLPTSAEGKGDPFNPEVSPVKLETHFEIP
                     ANDWRQAIRVSWYQGGAMPESPVKYLDLKKIDHGALFEGSKGCLVADFQSRAVFPHGN
                     QADLTYYKPRPKSALLPPLGNFQKQWINACKGDLKTACDFDYSGNMIEMMLLGLVAYR
                     VGKKIKYDGAAGHVTDCPEANELLARKYRPGWTLNG"
     gene            795..1613
                     /locus_tag="VIL39_11195"
     CDS             795..1613
                     /locus_tag="VIL39_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5482039.1"
                     /translation="MIRLLPRGVFARLAFVSLLAFCAVGCQSAQKLAASKTFRVMTYN
                     IHHGEGLDGKVDLQRIADLIKREQADIVALQEVDKGVKRTARRDCPAELAALTGMSCV
                     FSNNYHFQGGEYGNAVLTRFPIKRWTNRHYKMLRPGEPRGLLQVVLNVHGRDLVFLDS
                     HLDPRGDDAERLFSAAELVEAVQPYRGQPVILCGDFNDVPDSRTCQKLAQEFTDTWKA
                     AGAGDGFTIPAEKPRKRIDFIWTSKEPALEALKVWGPESEASDHLPVVAEFRLR"
     gene            1726..1896
                     /locus_tag="VIL39_11200"
     CDS             1726..1896
                     /locus_tag="VIL39_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482040.1"
                     /translation="MKRKKGQADKATAGTVIAAKYRARCNRLADSQREKLNEEFLQLY
                     YADGARQPARRP"
     gene            complement(2159..2335)
                     /locus_tag="VIL39_11205"
     CDS             complement(2159..2335)
                     /locus_tag="VIL39_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482041.1"
                     /translation="MRIYPDSSFLVSWLCSADKLNPRAKAWFYAHQNDDWVVSDWYQI
                     ALANARGFRAVSFI"
     gene            complement(2332..2592)
                     /locus_tag="VIL39_11210"
     CDS             complement(2332..2592)
                     /locus_tag="VIL39_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482042.1"
                     /translation="MKNSNTSDTLIYMTMLKKRELVRNPGLASHLRPGESIELEDRKE
                     PLVVLRRKKRTLTADEIHAELDRIWEGVPELDAQAALNDLRE"
BASE COUNT          537 a          838 c          839 g          517 t
ORIGIN      
        1 gcaaaccggg ctacctgccc gccgcaggcg acccgcccaa cgacttccac tgggacctgt
       61 ggctcggccc ttctcccgcg catccgtgga atccggaata cttctccggc ggctccggca
      121 tgaattgcct gcaatggaac atgtattggg acttcggcag cggccaggtc ggcgacatgg
      181 ggagccacac tatggacatt gtctggaacg ccattgacgc cggcctgccg acttcggccg
      241 aaggcaaagg cgatcccttc aaccccgagg tgtcgccggt gaagctggag acgcatttcg
      301 agatcccggc caacgattgg cggcaagcca tccgcgtctc gtggtaccag ggcggcgcta
      361 tgcccgagtc gccggtgaag tacctggacc tcaagaagat tgatcatggc gccctcttcg
      421 aggggagcaa gggctgcctc gtggccgatt tccaatcgcg cgccgtgttc ccgcatggca
      481 accaggccga cctcacttac tataagccgc ggcccaagtc cgccctgctt ccgccgttgg
      541 gtaacttcca gaagcagtgg atcaatgcct gcaagggcga cctgaagacg gcctgcgatt
      601 tcgattactc gggcaacatg atcgagatga tgctgctcgg gttggtggct taccgcgtcg
      661 gcaagaagat caaatacgac ggagctgcgg gccatgttac cgactgcccg gaggccaacg
      721 aattgctggc ccggaaatat cggcccggct ggacgctgaa cgggtagagc gactcgaaac
      781 ttggcccact gcgcatgatc cgcctcctgc cacggggcgt ctttgcccgc cttgcttttg
      841 tcagcctgct ggcgttctgt gccgtgggct gtcaaagcgc acagaagctg gcggcaagca
      901 agacgttccg ggtgatgacg tataacatcc accacggcga gggcttggat ggtaaagtgg
      961 atttacagcg catcgctgac ctgattaaac gcgaacaggc ggacatcgtc gccttgcagg
     1021 aagtggacaa aggcgtgaag cgcaccgcgc ggcgcgattg ccccgctgag ctggccgcgc
     1081 tgacgggcat gagttgcgtg ttcagcaaca actatcattt ccaggggggc gagtacggca
     1141 acgcggtgct gacgcgcttt cccatcaagc gctggaccaa ccgtcactac aagatgctcc
     1201 gccccggcga accgcgcggt ctcctacagg ttgtcctcaa tgtgcatggg cgcgacttgg
     1261 tcttcctgga ttcgcatttg gatcctcggg gggacgacgc cgagcggttg ttcagtgctg
     1321 cggaacttgt ggaggccgtc caaccatacc gcggtcagcc cgtgatcctc tgcggcgact
     1381 tcaacgacgt gcccgacagc cgcacctgcc agaagcttgc ccaggaattc accgatacgt
     1441 ggaaggccgc cggcgcgggc gacggcttca ccatccccgc ggagaagccg cgcaagcgga
     1501 tcgatttcat ctggacctcg aaggagcccg ccctcgaagc gctcaaggtc tgggggcccg
     1561 aatctgaggc gtccgatcat ctccctgtgg tagcggaatt caggttgcgg tgatcctgaa
     1621 gaggcggcac accaggccaa gtactggcgt gacacttgcc ctgtgccatc tcgccatctc
     1681 gccttttcgt cttgaagaac ccgagccagc cgctaaagta acgtcatgaa gcggaagaaa
     1741 gggcaggctg ataaagcgac tgccggcacg gtcatcgccg ccaagtatcg agcgcgctgc
     1801 aaccgactcg ccgacagcca gcgcgaaaaa ctcaacgagg agttcctcca gctttactat
     1861 gccgacggcg cccgtcaacc cgctcgtcgc ccttgacgcc aatgtggtga tggatttggg
     1921 cgagggatgc gacacggtcc tggatgccct cacaacgtcg acgtgaccgc accggtcatt
     1981 gccgcgcacg gcggagcacg cgtgggcgcg ggaagaggcg gcgcgactgc cggtgccatt
     2041 tctgacgagc gaggccgtcc tgacagaagc cgcttttctc ttggcaaggg acggcgatgc
     2101 tggggggctt tatcgcgtgg aaccgggtgt cccacgcgcg gctcgagggg aaggtgtttt
     2161 agatgaagga gacggcccgg aagcccctgg cgttggcgag ggcaatctga taccagtcgg
     2221 agacaaccca gtcgtcgttt tgatgggcgt aaaaccaagc cttggcccgc ggattgagtt
     2281 tgtccgcgct acagagccag gataccagga aagaggagtc ggggtagatt ctcattcccg
     2341 gagatcgttc agcgccgcct gggcgtccag ctcggggaca ccttcccaaa tgcggtcaag
     2401 ttcggcgtgg atttcatccg cggtcaaagt ccgcttcttg cgccgcaata ccaccagagg
     2461 ttccttgcga tcctcaagtt cgatggactc cccgggccga agatgggaag ccagacctgg
     2521 attgcgcacc agctcgcgct ttttcaacat cgtcatgtag attaatgtgt cagatgtatt
     2581 gctgtttttc aagccttcct cgaccagaca cgccgtcccg ctgaatcatt ccgccgccga
     2641 tctcgccgcc gaccacgcca cgctggaaag aattcttgcc cgctcgtgag cgtcctcgtc
     2701 tctgacactt cgccattaaa acgatagcgg g
//
LOCUS       DATCGS010000547         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_690516, whole genome shotgun sequence.
ACCESSION   DATCGS010000547 DATCGS010000000
VERSION     DATCGS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690516"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..351
                     /locus_tag="VIL39_11215"
     CDS             178..351
                     /locus_tag="VIL39_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482043.1"
                     /translation="MSDSPSSIRGLLYGKWVRPLGLWRTVDPLYIWSAVIGLSVAFVL
                     IVVAQVMQTGRLH"
     gene            485..679
                     /locus_tag="VIL39_11220"
     CDS             485..679
                     /locus_tag="VIL39_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482044.1"
                     /translation="MQKVIVALVVAAVALGGTLVADSRELARARDLYRHGDFLGAGKL
                     LETAGTLDAEGLLLAGQADY"
     gene            800..1372
                     /locus_tag="VIL39_11225"
     CDS             800..1372
                     /locus_tag="VIL39_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482045.1"
                     /translation="MLVAPKYAVAARQSFEEAVRLDPKNVEAWNDLFNYYIEAPRFLG
                     GGLDKAAAVADRVLGLDAAEYHSDRARLAEKQKQPKLAEHHYRMAAELAPQDPGRLID
                     LAEFLESQGRTEAADRAFDRAHEVAPGAPVVLYEHARTYVQSGRRLDDARALLIRYLS
                     INAGPDNPPRSEALKLLKRLPSASAANPAR"
     gene            complement(1688..>2408)
                     /locus_tag="VIL39_11230"
     CDS             complement(1688..>2408)
                     /locus_tag="VIL39_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5482046.1"
                     /translation="SITELVHDRYLYLPSIGYCSLLAFVLRWILGRVRGRAALAGMSV
                     IVLAALLYGYLVVVQSRPWADEVSLFRNSVMVAPSSNHAFQQFAYALAQQGHCADALP
                     LLATVVDHEPDNAKAVFGLGACYFHTGNLETAEALMLRTIELNSHYQQPYLLIATIRL
                     WGKRVDDAEWYWRKAVSAQQGPNEERTLHCVRGEILRARGDFRGAIAEFRKELEVQPG
                     NQEILAELNATEVLVSQSGHD"
BASE COUNT          495 a          709 c          726 g          478 t
ORIGIN      
        1 ttgggcggtg gctgccgccc aatgccgccc actggtgccg acccaaatgc catgtcctta
       61 gaccttcgga tgcagtttct ggtgtaatcg cacgaaagcg atgcgttcaa atggattgcg
      121 tgtatgcagt gatggccggc gctcaaggcc cagtagcacc aagaaagttg aggtcggatg
      181 tctgattctc ctagctcgat tcgggggcta ttgtatggca agtgggtcag gcctcttggc
      241 ctgtggcgga ccgtggaccc gctctatatc tggagtgcag tgatcgggct ctccgttgcc
      301 ttcgtgctga tcgtagtcgc ccaggtaatg cagacagggc gtctgcatta ggtctaccgc
      361 gaccgccgct atcgcgcctc ctgtacgaac tttgccgtat tgagggtgtc gaccatcacg
      421 gataagattg ctgtcagtgg gtgaacacat ctggaacgtg accgccggca gagcacttgt
      481 tcccatgcag aaagtgatcg tcgcactcgt ggtggcggcg gtcgcactcg ggggcacgct
      541 tgttgccgat tcccgagagc tagcgcgggc tcgcgatctt taccggcacg gcgactttct
      601 gggcgccggc aaactgctcg aaaccgccgg cacactcgat gctgagggat tgctgctggc
      661 ggggcaagcc gattattgag cgggcaatct ccgaggagcc tgtggcttct ttgagagggc
      721 agttgccgcc aatccagcgg cttcccgtta cgttcattgg ctgggacgcg cctatgggcg
      781 ccgggccgaa accgcaaata tgctggtggc acccaagtat gcagtggcag cccggcagag
      841 cttcgaagag gcggtccggc tagatccgaa gaacgtcgag gcgtggaatg acctgttcaa
      901 ttactacatc gaggcgccgc ggttcctggg gggaggactg gacaaggcgg cagcggtcgc
      961 agatcgggtc ctcgggttgg atgctgcgga gtatcattcc gatcgggcgc gtctggcgga
     1021 aaagcagaaa caacccaaac tggccgagca tcactaccgc atggccgctg aactggcgcc
     1081 gcaggatccc gggcgcctga tcgatctcgc cgaatttctg gagtctcagg gccggacaga
     1141 ggcggcggat cgggcttttg atcgggctca cgaggtcgcg cccggcgctc ccgtggtcct
     1201 atacgagcat gcgcgcacgt acgtccaatc aggacgacgc ctggatgatg ctcgcgcact
     1261 cctgatccgc tatctctcaa tcaacgccgg tccggataac ccgccgcgaa gcgaggcgct
     1321 caaactgctg aagcgactgc ccagcgcgtc ggcggcgaac ccggcaagat aggcgcgcag
     1381 gtaacgcttt caagcagcca atcccaaatt gttccgcccg gcttccgtca gtcgcgcttt
     1441 gccgtgcggc ggttgctcga agaggcggca gcctaaacca gccgagtaca ggaccggaag
     1501 catcatgtct tatcggtcgt gtccggcgcg atcaggcacc ctcgccgaaa gctcaacgaa
     1561 gcttcactac ggcgcgatgc aagcgcgcga gcgatgcggt gccaaacctc aagcaggcga
     1621 tggagattcc tacggtctaa gctcaacgat ttaccttggt cttgagcccg acagcacctg
     1681 cgagaactca atcatggcct gattgtgata ccagcacctc cgtggcgttc aattccgcca
     1741 ggatctcctg gttccccggc tgaacctcca attccttgcg gaattcggcg atggcacctc
     1801 gaaaatctcc cctggctctg agaatttctc cacgaacaca gtgtaaggta cgctcctcgt
     1861 tgggaccttg ctgcgcactg acggcctttc gccagtacca ctcggcatca tccacacgct
     1921 tcccccacag acgtatggta gcaatcagga ggtaaggctg ctggtaatgc gagttgagct
     1981 caatagtccg cagcatgaga gcttcagcgg tttccaggtt tcccgtatgg aaatagcagg
     2041 cgcctagtcc aaatacggct ttggcattgt cgggttcgtg gtctaccacc gtggcgagga
     2101 gcgggagggc gtcggcgcaa tgcccttgct gggccaacgc gtatgcaaac tgttggaagg
     2161 cgtggttgct cgaaggagcc accattacgc tgttgcgaaa aagcgagact tcgtctgccc
     2221 atggacggct ttgcaccacg accagatacc cgtacagtag agctgccaag acgataaccg
     2281 acattccggc tagcgctgcc ctacccctga cccggccaag aatccaccgc aacacaaaag
     2341 ccagcaacga acaataacca atggagggca aatacaagta acggtcgtgc accagttccg
     2401 ttatcgaa
//
LOCUS       DATCGS010000548         5904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_146523, whole genome shotgun sequence.
ACCESSION   DATCGS010000548 DATCGS010000000
VERSION     DATCGS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146523"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VIL39_11235"
     CDS             <1..335
                     /locus_tag="VIL39_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY5482047.1"
                     /translation="LALAMFSMLKRELTANPRRQLGALLAQNAFRTIKRRMDPEGFGG
                     APLLGFNGTVMKAHGSARERAVANAIRVTTENIQHRVSQIIADEIARATERLAANETV
                     AATLLLEN"
     gene            353..1390
                     /locus_tag="VIL39_11240"
     CDS             353..1390
                     /locus_tag="VIL39_11240"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5482048.1"
                     /translation="MSPLSAPKFKNPRAKYNFQGRTCSISGVGSYVPARILTNAELEK
                     MVDTSDEWIITRTGIRERRLAAKGEFTSDLATQAALRAMERAGVTAEQIDLIIVATIT
                     PDMPFPSTACLVQRKIGAYRAAGFDLEAACSGFIYGLEVAQQFIMSRTYDTVLVIGAE
                     KLSSIVDWKDRNTCVLFGDGAGAAVLQNRPNAHGLLTAVMGADGRKADLIHMAGGGSR
                     CPATAESVASGMHYLRMEGKETFKTAVQAMKTAAQEALRRCEIDISRIKCIIPHQANR
                     RIIDAVLERLGAKPEQLFVNLHKYGNTSAASVAIALDEAVASGLVQRGDLILMVVFGA
                     GLTWGAAVIEW"
     gene            1460..1786
                     /locus_tag="VIL39_11245"
     CDS             1460..1786
                     /locus_tag="VIL39_11245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5482049.1"
                     /translation="MSARKIESTGSLQAVTLEARQTRLTLSPNSVVIHKNGIEFRSAT
                     PFSEWAEMTMTLQSPGDGGKVHCTGVVISCTGNKHAGYHVSMVFTGLSKQAQARLTTM
                     AYAPLG"
     gene            1907..2692
                     /locus_tag="VIL39_11250"
     CDS             1907..2692
                     /locus_tag="VIL39_11250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5482050.1"
                     /translation="MSVETVLVTGASSGIGLELARCFAADGCRLALVARKGDALEALA
                     TELRKAHKIQAQVFTADLAHPEAPPRLLAHLQSAGIKVDVLVNNAGFGAQGKFADLPL
                     GRQLEMLQVNITSLTHLTGLLLPGMIERRRGGILNVASTAAFQPGPGMAVYYATKAYV
                     LSFSEALAEELSGTGVTVTASCPGPTATNFGAAANMKTLGLVKKISMSAKPVAQISHR
                     AFRRGSVVAITGFRNQFPTFLVRLFPRAVVRKITKRLNGVKYE"
     gene            complement(2816..3817)
                     /locus_tag="VIL39_11255"
     CDS             complement(2816..3817)
                     /locus_tag="VIL39_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5482051.1"
                     /translation="MQIIITPGQLSRRADFYHQLAQLTGAGLGLLQTLEQLKRNPPDR
                     SYREPITRALEQIADGCTFTEAVQRCNHWLPAFDLALLEAGEHSGRLDACFRLLADYY
                     TDRARLARQVIGDLAYPAFLLHFAIFIFPFAQFFASGNWAVYLLQTVGLLIPLYVTVA
                     LLVFAAQSRHGETWRACFETLLHPIPVLGGARRYLALARLAAALEALLSAGVTIIEAW
                     ELAATASGSPALRRMVLGWRPQVNAGQTPAEAVSAAGSFPDLFVTQYATGEISGQLDE
                     TLRRLHAYYQEEGSRKLHAFARWTPRAIYFCIVLMIAYRIIAFYTGYFNMVRDAGGF"
     gene            complement(3956..4288)
                     /gene="trxA"
                     /locus_tag="VIL39_11260"
     CDS             complement(3956..4288)
                     /gene="trxA"
                     /locus_tag="VIL39_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5482052.1"
                     /translation="MAAPNIVDLTQENFAAEVLKASMPVLVDFWAEWCGPCKMIGPVL
                     DELAAEYAGQVKIGKVNIDQQQVLAAQYGVRAIPTLLLFHKGEVADQMVGLRSKRDLK
                     ASIARVAP"
     gene            complement(4379..4798)
                     /locus_tag="VIL39_11265"
     CDS             complement(4379..4798)
                     /locus_tag="VIL39_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482053.1"
                     /translation="MAENIPNIPSGAVPPKKETGKVQPKKETVRINLPPKPSAAPTIK
                     LPTLPPGGPGGGPSASAPGAPSAAAPAHPLSAPRASAAAPAAPRSAPSAPRPAMAAAP
                     ARPAASGLDTILAIAAAIVGLAAVGSTVYLWLFLPNL"
     gene            4997..>5904
                     /locus_tag="VIL39_11270"
     CDS             4997..>5904
                     /locus_tag="VIL39_11270"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5482054.1"
                     /translation="MTVQQQEAIFWREVGEVGVAAFPTWLFLHWAIRDLRGVLSPSEG
                     ERGLTRVPWLTPVPAAGSGGLRLLFGILLVSLSTICVLTASAVPSTPAAGAQALEVIR
                     KKHDLPALAVVVVKDGQICDRAAVGVRKSGESTPVTTNDVFHVGSCTKSMTATLTALL
                     IEGGQLRWDTTIADVFPELKGKMDKQYEAVTVEQLLTHRGGVPGAPPAAAWKRAWEQK
                     GTPTQQRREFIEAVLSQPPEAAPGTKMIYSNQGYAILGAMLEKLTGTAWEALITERLF
                     KPLHMDSAGFGPPGATGAIDQPWGHTR"
BASE COUNT         1174 a         1880 c         1767 g         1083 t
ORIGIN      
        1 gcctggcgct ggctatgttt tcgatgctta aacgcgaact gacagccaat ccccgacggc
       61 agctcggagc cttgctcgcc caaaacgctt tccgcacgat taaacgccgc atggatcccg
      121 aaggttttgg aggcgcgccg ctgcttggct tcaacggcac agtgatgaaa gcccacggct
      181 ccgcccgaga acgggccgtt gccaatgcca ttcgcgttac caccgagaac atccagcatc
      241 gggtgagcca aatcatcgcg gacgagatcg cgcgtgccac tgaacgcctg gctgcgaacg
      301 aaacggttgc cgcaactctg ctgttggaga actagataac gccaatgcca ccatgagtcc
      361 attgtccgct cccaaattca agaatccgcg ggccaagtat aacttccagg gccgaacctg
      421 ctccatcagt ggcgtgggct cctacgtgcc cgcgagaatt ctcaccaatg ccgagctgga
      481 aaaaatggtg gacacgtctg acgagtggat tatcacgcgc acaggcatcc gagagcgccg
      541 gctcgcggca aaaggcgaat ttacctccga cctggccacc caggccgcgc tccgcgcgat
      601 ggagcgtgca ggcgttaccg cggaacaaat agaccttatc atcgtggcca ctatcacgcc
      661 ggacatgccg ttcccctcaa ccgcgtgcct ggtgcagcgg aagatcggcg cctaccgtgc
      721 cgcgggcttc gatctggaag cggcttgttc gggcttcatc tacggacttg aagtcgcgca
      781 gcagttcatc atgtcccgca cctacgacac cgtcctggtc atcggtgcgg agaaactatc
      841 ttcaattgtg gactggaagg accgcaacac ctgcgtattg tttggcgacg gtgccggcgc
      901 agcggtgttg cagaatcgcc ccaacgcgca tggcttgctg acggcggtta tgggcgcgga
      961 tggccggaaa gcggacctga ttcacatggc cggcggcggc agccgttgcc cggcgacggc
     1021 ggagtcggtc gcctcgggca tgcactacct gcgcatggag ggcaaagaaa ccttcaagac
     1081 cgccgtgcag gcgatgaaaa ctgccgccca ggaagcactg cgccgctgcg agatcgacat
     1141 ttcgcgcatt aaatgcatca tcccccacca ggccaaccgt cgcatcatcg atgccgtcct
     1201 cgaacgcctc ggcgccaaac ccgaacagct ctttgtcaac ctccacaaat acggaaacac
     1261 ctccgccgct tccgtggcca ttgccttgga tgaggccgtc gcatccgggc tcgtccagcg
     1321 tggcgacttg atcttgatgg tggtttttgg cgcggggctc acgtggggcg cggcggtaat
     1381 tgaatggtaa actcttatta ttatatttaa aacagctaat ttgacacttt agccgggtag
     1441 gttattctga tattgcccta tgagcgcgag aaaaattgaa agcactgggt cgttgcaagc
     1501 ggtcaccctt gaagcacggc aaacccgctt aaccctctct cccaactcgg tggtcatcca
     1561 caagaacggc atagaattcc gcagtgccac acctttctcg gagtgggccg agatgacaat
     1621 gacgctgcag tcgccgggcg acggcggcaa ggtccactgc acgggggtcg tcatctcctg
     1681 caccggcaac aagcacgctg gctatcacgt ctcgatggtt ttcaccggcc tctccaagca
     1741 ggcgcaggca cgcctgacca cgatggccta cgcccccctc ggatagtttc ttcccaccgc
     1801 aagaacggtt tgccctctgg cagtataagg tcaggctgcg ccccaacgca cccctgagtt
     1861 ttctgcgcgg ttgcaaaacc aggcgccgta aggttacctg aggactatga gcgtcgaaac
     1921 agttcttgtc accggagcgt cgtcgggcat tggtcttgag ctggcccggt gcttcgccgc
     1981 tgacggctgc cggctcgccc tggtcgcccg caaaggcgat gcgctcgagg cgctggcaac
     2041 ggagctgcga aaggcccaca agatccaggc gcaggttttc accgccgacc tcgctcaccc
     2101 ggaagctccg ccccggctac tcgcccacct gcagagcgcc ggcatcaagg tggacgtgct
     2161 ggtcaacaat gcgggcttcg gcgcgcaagg caagttcgcg gacctgcccc tcgggcggca
     2221 gcttgagatg ttgcaggtga acattaccag cctcacgcac ctcacgggtc tgctgctccc
     2281 cggcatgatc gagcgccgcc gcggcggcat tctcaacgtc gcctcgaccg ccgccttcca
     2341 acccggcccc ggcatggcgg tctattacgc gactaaagcc tacgtgctgt ccttcagtga
     2401 agccctggcc gaggaactgt ccggaacggg agtgaccgta accgcatcgt gcccgggccc
     2461 cacggcgacg aacttcggcg ccgccgccaa catgaaaacc ctggggctag taaagaagat
     2521 ctccatgtcg gctaaaccgg ttgcccaaat cagccatcgc gctttccgcc gcggcagcgt
     2581 cgtggccatc acgggttttc gtaaccagtt ccccacgttc ctggtccgcc tcttccctcg
     2641 cgcggtagtc cgcaagatca ccaaacgcct caacggcgtg aaatacgagt aggtacctgc
     2701 agtgattcac gcgccgccag cttcagtgcg accgccagac ccatcacgat gcagatgtgc
     2761 ttttggccca attccatggt tgccgtgtta agccattgtg cggttgctgg acgcttcaaa
     2821 acccacccgc gtcccgcacc atgttgaagt agcccgtgta gaaggcgatg atgcggtagg
     2881 cgatcatcag cacgatacag aaatagatgg cgcgcggcgt ccagcgcgcg aatgcgtgga
     2941 gtttgcgcga gccttcttcc tgataataag cgtgcagccg ccgcagagtc tcgtccaact
     3001 gtccgctgat ttcgccggtc gcgtactggg tgacaaagag gtccggaaag ctcccggcag
     3061 cgctcacggc ctcagcgggc gtctggccgg cgttcacctg cggccgccac cccagcacca
     3121 tccggcgcag cgcgggcgaa ccgcttgcgg tcgccgccaa ttcccaggct tcgataatcg
     3181 tcacccccgc gctaaggagc gcttcgagcg cggcggccag ccgtgccagc gccaggtaac
     3241 gtcgcgcccc gcccagcacc gggatcggat gcaggagcgt ctcgaaacac gcgcgccacg
     3301 tttccccgtg ccggctctgc gccgcgaaaa ccagcagcgc gaccgtcacg tagaggggaa
     3361 ttagcaggcc gaccgtctgg agcaaataaa cggcccaatt gccggaggcg aagaattgcg
     3421 cgaacggaaa gatgaagatc gcaaagtgca gcaggaacgc cggataggcc aggtccccga
     3481 tgacctggcg cgcgagccgg gcgcggtcgg tgtaataatc ggccaacagc cggaagcagg
     3541 catccagtcg gccgctgtgc tcacctgcct ccagcagcgc gaggtcgaac gccgggagcc
     3601 agtggttgca gcgctgcacc gcctcggtga acgtgcagcc gtcggcgatc tgctcgagcg
     3661 ctcgcgtaat gggctcgcgg taggagcggt cggggggatt gcgtttgagc tgctccagcg
     3721 tctggagtaa acccagcccc gcgcccgtaa gctgggcgag ctgatgatag aaatcagcgc
     3781 ggcgcgacaa ctgtcccggc gtgataatga tctgcatagt tcgagcgcaa ctccggcttt
     3841 cctgccggcg ggttgcgagg catcttgcgc tgcccgccta tcccagcgca agccacgtca
     3901 ccggatgcca cgcccggttt tgggtggccg aacacgccgg gcgcgcaact ccggttcagg
     3961 gagctacgcg ggcgatactg gctttgagat cgcgcttgct gcgcaggccg accatctggt
     4021 ccgccacctc ccccttatga aagagcagga gcgtggggat ggcgcggaca ccatactggg
     4081 ccgccagcac ctgttgctgg tctatgttca ctttgccgat cttgacctga ccggcatatt
     4141 ccgcggccag ttcgtccagc accggcccga tcattttgca cgggccgcac cactccgccc
     4201 agaaatccac cagcacgggc atggaagctt tcagcacttc cgcggcgaag ttctcctgcg
     4261 tcaaatctac aatgttagga gctgccataa gcaatagaat gaaactcagg ttgattgcac
     4321 cactaaataa agacccgttg cgcccgcgct tctgacctca agtcagatca gcgcccgctc
     4381 acaggttcgg cagaaagagc caaaggtaaa ctgtgctccc caccgctgcc agtccaacga
     4441 tggctgcggc aatcgccagg atcgtgtcca gcccgctggc ggcgggtcgg gcgggggcgg
     4501 cggccatcgc agggcgcggc gcactgggcg ccgaacgcgg cgcagccggg gcggccgcag
     4561 aggcccgggg cgcactcaac ggatgagccg gcgccgcggc gctcggggct cccggcgcgg
     4621 acgccgaggg tcccccgcca ggtccccctg ggggcagggt cggcagtttg atagtcggcg
     4681 cggctgaggg cttgggcggc aagttgatgc ggacagtctc tttcttgggt tggactttgc
     4741 ccgtttcctt cttcggcgga actgccccgc tcggaatgtt aggaatgttc tcggccataa
     4801 aatatattgt gctctaaatt aaagttgccg tcggcgagtg tcaaaccctt attccaacag
     4861 aaaaaccggg ccacagggca cgttcgggga atcgtccccc caccttatcc tcaaaagtgc
     4921 atttcctttg taagaaacga cgaagttcgg tcattatgtg actgaacaca gacagattca
     4981 atgaccgttc aacaacatga ccgttcaaca acaggaagcg atattttggc gcgaggtggg
     5041 ggaagtgggc gtggcggcct tcccaacctg gctgtttttg cattgggcca tccgtgacct
     5101 gaggggcgtc ctctccccct cagagggcga gagggggctc acgcgagttc cttggttaac
     5161 tcccgttccg gcagccgggt ccggaggtct gagacttcta tttggaatcc tgttggtttc
     5221 actctcgact atctgcgttc tgactgcatc tgctgtccca tcaacgcctg cggccggagc
     5281 ccaggcactc gaagtcatcc gcaagaagca tgacctgccg gcactcgcgg tggtggtggt
     5341 gaaagacggc cagatttgcg accgcgctgc cgtgggtgtc cgcaagtcgg gcgagtccac
     5401 gccggtcaca acgaacgacg tattccacgt cggttcctgc accaagtcca tgacggccac
     5461 actcaccgcc ctgttaattg aaggtggtca attgcgctgg gacaccacga ttgccgacgt
     5521 gtttcccgag ctgaagggca agatggacaa gcagtatgaa gctgtgacgg tcgagcaact
     5581 gcttacccat cgcggcggcg ttcctggcgc accgcccgct gccgcatgga aacgggcttg
     5641 ggaacaaaaa ggcacgccca cccagcagcg ccgtgagttc atcgaagcgg tcctgagtca
     5701 accgccggaa gccgcgcccg gaacgaagat gatctattcg aaccaaggct acgccatcct
     5761 cggcgcgatg ctggaaaagc tcaccggcac ggcctgggaa gcattgatca ctgaacggct
     5821 cttcaagcct ttacacatgg actccgccgg cttcgggccg ccgggagcaa ccggtgcgat
     5881 tgaccagcct tggggtcaca cccg
//
LOCUS       DATCGS010000549         8963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_392258, whole genome shotgun sequence.
ACCESSION   DATCGS010000549 DATCGS010000000
VERSION     DATCGS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392258"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VIL39_11275"
     CDS             complement(<1..325)
                     /locus_tag="VIL39_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5482055.1"
                     /translation="MRIALVNDMMAAVEALRRVILGTREHEVAWVARDGAEALDLCAS
                     DAPDLILMDLIMPGMDGVEATRRIMANQPCAIVVVTANVATRSSKVFEAMGAGALDAV
                     NTPVLE"
     gene            complement(322..2658)
                     /locus_tag="VIL39_11280"
     CDS             complement(322..2658)
                     /locus_tag="VIL39_11280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY5482056.1"
                     /translation="MSAPLPQDPSNLPMMELFRAEVENQTAILTNGLLELERGQGAPQ
                     QLEVLMRAAHSFKGAARIVDLPAAVRVAHAMEDCFVLAQQGKLALRQPEIDLLFRGVD
                     LLLHISKHTEANITRWETDHADEIREFLNSLVRLTPVGKTPAPSSSQPAAYTGPAAPG
                     GSTGPHPPGDLSLPAAPESAPLEDRSQPVVTRRHETPERVVRLTAENLNRLLGLAGES
                     LVESRWLRPFTDSLRRLTRQHTELADKLDDLRQRFGETDLSGRAKQQLVELSHKVAGC
                     QQFLSSRLQELDLFDRRSAHLSQRMYLEVLRTRMRPFSDGIRRFPRMVRDLSRIMGKQ
                     VRLEIIGEDTQVDRDILERLESPLTHLLRNAVDHGCETPEQRQHAGKSPKGTIRLEAR
                     HSAGMLLVTVADDGAGVQPERVREMILKRKLAAPAVAERLTEAELLEFLFLPGFTLKE
                     TVTEISGRGVGLDVVQNMVRSVRGSLRVSTQPGRGLRVQVQLPLTLSVLRALLVEVAG
                     EPYAFPLTQITRALKLQHEDIETLESRHHFHFEGQQVGLLAAHQIFDCGPPKPPANDL
                     PVVVLGERNARYGLVVDRFLGERELVVQPLDGRLGKVKDISAAALMEDGSPVLIVDVD
                     DMVRSIEKLLSEGLLTEVQPKGLDLTGRKRKRVLAVDDSLTVRELVRKLLANRGYLAD
                     VAVDGMDGWNAVRTGNYDLVITDVDMPRLDGIELAALIKKDPHLKSLPVMIVSYKDRE
                     EDRLRGLEAGADYYMTKGSFHDETLLQAVVDLIGEPDT"
     gene            complement(2655..3386)
                     /locus_tag="VIL39_11285"
     CDS             complement(2655..3386)
                     /locus_tag="VIL39_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5482057.1"
                     /translation="MSTSSLQINEPASSDDRSRSLPNSQAVEIRPCWNEIGVQGDATC
                     PELQQVIHCRNCAVYSKAGVQLLDRPLLPEYRRAWTEHFAQERKLTGSARQSALLFRI
                     SSEWLALPTPAFQEVAERRPVHSLPHRRQGIVLGLVNVRGELLICVSLDHLLGLAKSP
                     LRETPHTTYDRLLVAKWDRHRFVFPADEVRGILRFHAPELQEPPATLTKSRLSYTQGV
                     LRWQERAVGLLDADLLFSSLNRSLM"
     gene            complement(3394..4629)
                     /locus_tag="VIL39_11290"
     CDS             complement(3394..4629)
                     /locus_tag="VIL39_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY5482058.1"
                     /translation="MKQFEHLLRETIGLDAQSIGPTSIQRAVRLRMRSLGLKSPEDYQ
                     QLVERSRAEWNALVESVIVPETWFFRDPALFSAFAHHVLAEWLPAHPAVPMRVLSIPC
                     ASGEEPYSLVMALLDAGVAPERFQIHAVDISARALACAESGLYGNNSFRGQDLAFRDR
                     YFQPSGEGFVLAAAVRSCVRFYRGNLFSDDFRPKLASYNFIFCRNLLIYFDRPTQQKA
                     IETIERLLAPFGVLFVGPVEQPLVLSHGLVSANIPAASACRKAAHVVRRPRLARIIKQ
                     PSVPAAPQPNGALQPHLAAGSGLNLPTAARPLPSVRHDLETARRLADAGRLHEAAEIC
                     EAHLRQSRGSAQAYYLLGLVRDASGDARAIECYRKALYLEPNHYESLLQMALLLQKNG
                     DPSRARAFTNRAQRIIAKG"
     gene            complement(4616..5092)
                     /locus_tag="VIL39_11295"
     CDS             complement(4616..5092)
                     /locus_tag="VIL39_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5482059.1"
                     /translation="MLFLLFQLGKERYALQASRVVEVVPLLELKQLPQAPKGVAGIFN
                     YHGRPVPAVDLCALTLGQPASERFSTRIIIVNYPDDRGISHLLGLIAEHATEMLRKEP
                     NEFMDSGVKLSGAPYLGPILMDSQHPVQWIYEQRLLPERVRDLLFSEPAALCHETV"
     gene            complement(5095..6693)
                     /locus_tag="VIL39_11300"
     CDS             complement(5095..6693)
                     /locus_tag="VIL39_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5482060.1"
                     /translation="MKTVTIWQRLNIAFWLLVALLLVGVGLALWAADAGSARVRRGDQ
                     LSEAKNSIKYDVVFISDTVRGLLLDPKNVAEEKRKLVAESDLGSQLRTIQSEFTDYPD
                     LIRSVSNLSEFASRTLTGFHRQVLATAETDSASAIMLYNKEYPDIREKRVRLFTDLTF
                     EIERVRNAESMRGARLASVGLTPIIILLLLAIPIVGRVHSSTVAKPLNRLVAALERMR
                     QGDFTERLTLQRRDEFGVLGNGLNRLADDLSELVGQVQRSGIQVNTTATEIAATAREQ
                     QSTAHEIAATTAEIGATSKQISATSKELVKTMNEVNQVAEQTADLAGSGRTALTRMET
                     TMRQITEASGSITAKLSVLSEKTTNINSVVTTITKVADQTNLLSLNAAIEAEKAGEYG
                     LGFAVVALEIRRLADQTAVATYDIEKMVKEMQSAVAAGVMGMGKFSEEARRGVEEIRH
                     VSTHLAQIIHQVQTLTPRFQTVNEGMHAQATGAQQISDTLTQLSEAAQQTAESLRQSN
                     LAIEQLNSAARGLQTSVARFKLAV"
     gene            7173..8228
                     /locus_tag="VIL39_11305"
     CDS             7173..8228
                     /locus_tag="VIL39_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482061.1"
                     /translation="MAALAGCDRGQVQEYRVAKEQTQAQPSAMPPGHPDTSGAASPVL
                     KYKVPTAWQEVVPGEMRAASFRVADKQADVSVIPLPGLAGSDLDNVNRWRGQVGLPGV
                     PEAELAALAQPVEIAGQAAKLYEQAGANAGSGEKSRILAAITRREGAAWFFKMTGEDG
                     VVAEQKPAFIEFLKSVSFTAAGAQAQLPPSHPPIDGGSMMAAAGAAPSSGQAKPNWEV
                     PSGWKEIPGGQFLVAKFVLTGAANALASVNVSMSSGDGGGMVANVNRWRNQLGLGPEA
                     EADLTKELQPLDLPGGKATLADITGQDAKTGQKARLLAVVVPRSGETWFYKLMGDAQI
                     VEREKDAFIKFVQTVKY"
     gene            8242..>8963
                     /locus_tag="VIL39_11310"
     CDS             8242..>8963
                     /locus_tag="VIL39_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ResB protein required for cytochrome C
                     biosynthesis"
                     /protein_id="HEY5482062.1"
                     /translation="MPISLDQFLDRLIKVFTSLRLTVVCLALGLLLVFFGTLAQVDLG
                     LYKAQNEFFRSFLIYWGPKGASWKVPIFPGGYLIGGVLLINLFASQFKRFVFTPSKIG
                     LWMVHVGLILLLLGQLLTDLLSRESTLHLREGEARNYSETERDAELAVSDVTDPATDK
                     VVAITQGVLARQQNIRPGDLPVMVQVKQFFANSTVEERAASAAQPPAATQGIGPRATV
                     KELPRVTEMDRRDVPSAVVEIVT"
BASE COUNT         1687 a         2656 c         2908 g         1712 t
ORIGIN      
        1 gttcaagaac tggcgtgttg accgcgtcca gggcgccggc acccatggcc tcgaatacct
       61 ttgacgagcg ggtggcaaca ttcgcagtga ccaccacaat agcgcaaggc tggttggcca
      121 tgatgcggcg agtggcttcg actccatcca tgcccggcat gatcaaatcc atcaggataa
      181 ggtccggggc atcactggcg caaagatcaa gggcttcggc gccgtcgcgc gcgacccagg
      241 cgacctcatg ttcacgcgtg ccgaggatca cgcggcgcag agcttcgacc gctgccatca
      301 tgtcgttgac cagtgcgatt ctcatgtatc gggttcgcca atcaaatcca cgacggcttg
      361 caagagcgtt tcatcgtgaa agctgccctt ggtcatgtaa taatctgcgc ccgcttccaa
      421 gccgcgcagc cggtcttcct cgcggtcctt gtaggaaacg atcatgacgg gcagggactt
      481 caaatgcggg tctttcttga ttagagcggc caactctatg ccatccaggc gcggcatgtc
      541 cacgtcggta atcaccaggt cgtaattgcc agtgcgcacg gcgttccacc catccatgcc
      601 atccacggcc acatcggcca gatagccgcg gttggcaaga agcttgcgga ccaactcgcg
      661 cacggtaagg gagtcgtcca ccgccagaac gcgtttccgc ttccggccgg tcaaatcgag
      721 gcctttgggc tggacttcgg tgaggaggcc ctccgaaagc agtttctcga ttgatcggac
      781 catgtcatcg acatcgacaa tcaggacggg ggagccgtct tccatgagcg ctgccgcgct
      841 gatgtctttg accttaccaa gccggccatc gagcggctgc acaaccagct cgcgttcccc
      901 cagaaagcgg tccacgacca acccgtaacg cgcgttgcgc tctccgagca cgacgacggg
      961 cagatcgttg gcgggcggtt tgggaggccc gcaatcgaag atctggtggg cagccagcaa
     1021 acccacctgc tgaccctcga aatgaaagtg gtggcggctt tcgagggttt cgatgtcttc
     1081 gtgctgcaat ttcaacgccc gggtaatctg agtcagtgga aaggcataag gctcgccggc
     1141 cacttccacc aacaaggcgc gaaggacgga gagtgtgagc ggcaactgca cctgaacgcg
     1201 caggccgcgg ccgggttgcg tgctgacgcg aagactgccg cgcacgctcc gaaccatatt
     1261 ctgcaccacg tcgaggccga cgccgcggcc ggagatctcg gtgacagtct ccttgagcgt
     1321 gaaacccggc aggaaaagga attcgagcaa ctcggcctcg gtgagccttt cggcgacggc
     1381 cggggccgcc agtttccgtt tcaggatcat ctcgcgcacg cgctccggtt ggacgccggc
     1441 gccgtcgtcg gcgaccgtta ccagcagcat gccggcgctg tggcgggctt cgaggcggat
     1501 ggtgcccttg ggggatttac cagcgtgctg gcgttgctcg ggtgtttcgc aaccgtgatc
     1561 cacggcgttg cgcaagaggt gggtcagcgg actctccaat cgctccagga tgtcgcgatc
     1621 cacctgggta tcctcgccga tgatttcgag ccgcacctgc ttgcccataa ttcgtgacag
     1681 gtcgcgcacc atgcggggaa aacgacggat gccatcgctg aagggccgca tccgagtgcg
     1741 cagcacttcc agatacatgc gttgcgaaag gtgcgccgag cggcggtcaa acaggtccaa
     1801 ctcctgcaga cggctgctca gaaactgctg gcagccagct actttgtggg acaactcgac
     1861 aagctgttgt ttggcgcgcc cggacaagtc tgtttcgcca aaacgctggc gcaagtcatc
     1921 cagcttatcc gccagctctg tatgctggcg tgtgaggcgc cggagggagt cagtgaatgg
     1981 acgcagccag cgagattcaa ccaaggactc acccgccagg cccagcagcc gattgaggtt
     2041 ctccgccgtg agccgcacga cacgttcggg tgtttcgtgc cggcgcgtga cgaccggctg
     2101 cgagcgatcc tccaggggcg cagactccgg ggcagccggg agggacaaat cccccggtgg
     2161 atggggcccg gtcgaaccgc cgggagcggc cggccccgtg tatgcggcgg gctgactgga
     2221 ggagggcgcc ggggtcttgc cgacgggtgt caacctcacc aatgaattga ggaattcccg
     2281 gatttcatcc gcgtgatcgg tctcccagcg ggtgatattt gcctccgtgt gcttggaaat
     2341 gtggagcagc aaatccaccc cgcggaacag caagtcaatt tccggctggc gcagagcaag
     2401 ttttccctgt tgagccagga cgaagcaatc ctccatggcg tgggcgacgc gcacggcggc
     2461 cgggagatcc acaatgcgag ccgccccttt aaacgagtga gccgcgcgca taaggacctc
     2521 caattgctga ggcgctccct ggccacgctc gagttcgagc aaaccgttgg tcaggatggc
     2581 ggtttggttt tcaacttccg cgcggaagag ctccatcatc ggcaagttgc tcgggtcctg
     2641 ggggaggggc gcgctcacat caggctccgg ttaagggacg aaaagagaag atcggcatcg
     2701 agcaggccga cggcccgttc ctgccagcga agaacacctt gcgtgtaact cagcctggat
     2761 ttcgtcaagg tggctggcgg ctcctgcaat tcgggggcgt gaaaccgaag gatgccgcgg
     2821 acctcatccg ccggaaagac gaagcgatgg cggtcccact tggccaccaa cagccggtcg
     2881 taagtcgtgt gcggtgtttc gcggagggga ctcttggcca gccccagcag gtggtcgagg
     2941 gagacgcaaa tcaacagttc accgcgaaca ttgacgaggc ctaacacaat gccttgccgc
     3001 cgatgtggca acgaatgcac cggccggcgc tcagctacct cctgaaacgc tggcgtgggc
     3061 aacgcgagcc attcagagct gatgcggaag agaagcgcgg attgcctggc cgagccggtg
     3121 agcttccttt cctgcgcgaa atgctccgtc cacgcgcgcc ggtactccgg cagcagcgga
     3181 cggtccagca actgaacgcc ggccttggaa taaaccgcac agttgcggca atggataacc
     3241 tgctgcaact ccgggcaagt ggcgtcgccc tgcacgccga tttcgttcca gcaaggccga
     3301 atctccaccg cttggctgtt tggcagcgac cgcgaccggt cgtccgagga ggcgggttcg
     3361 ttgatctgga ggctggaggt gctcacaacg cagtcacccc ttcgctataa tgcgttgagc
     3421 gcggtttgtg aacgcgcgcg cgcgggatgg gtcgccgttc ttctgcaata acagcgccat
     3481 ttggaggagg ctttcgtagt gattgggctc gaggtagagg gctttgcggt agcattcgat
     3541 tgcgcgggcg tcgccactgg cgtcgcgcac caggccgagc agataatacg cctgggcgga
     3601 tccccggctt tggcgcaggt gggcttcgca aatctctgct gcttcgtgca aacgtccggc
     3661 atcggcgagg cggcgcgcgg tttccaggtc gtgccggacg gagggtaatg gtctggccgc
     3721 ggtcggaagg ttcaatccgc tgcccgcagc gaggtgcggc tgcaaagctc cgttgggctg
     3781 gggcgcggct ggaacggacg gctgctttat gatccgggcc agccgcgggc ggcgcacgac
     3841 atgagccgct ttgcggcaag cggaagccgc ggggatattg gcactgacca acccatgact
     3901 cagcacgagc ggctgttcga ccggaccgac aaacagaacg ccgaagggcg ccagcaagcg
     3961 ttcgattgtt tcaatggcct tctgctgcgt gggccggtca aagtaaatga ggaggttgcg
     4021 gcagaagatg aaattgtagc tggccagctt gggccggaaa tcatcgctga acaagttgcc
     4081 ccgataaaag cggacgcaac tgcgaacggc agcagccaag acaaacccct ccccggatgg
     4141 ctggaaatag cggtcacgaa aagcgaggtc ctggcctctg aaggaattat tgccataaag
     4201 gcctgattcg gcgcaggcga gcgcgcgagc gctgatatca acggcatgga tctggaacct
     4261 ttcgggcgcc acacccgcgt ccaggagcgc cattaccaac gagtaaggct cttcgccgga
     4321 ggcgcaaggg atactgagca cgcgcatagg gacggctgga tgggcaggca gccattcggc
     4381 gagcacatgg tgagcgaaag cagagaagag cgccgggtcc cggaagaacc aggtttcagg
     4441 gacgatcaca gactcgacga gtgcattcca ttccgcgcgc gagcgttcca cgagctgctg
     4501 gtaatcttca gggcttttca ggccgagact cctcatgcgc agccggacgg cgcgttgaat
     4561 ggatgtcggg ccgatggatt gggcatcgag gccgatcgtc tctctcagca gatgctcaaa
     4621 ctgtttcatg gcatagggct gcgggctcgg agaacaacaa gtcgcgaacg cgctcgggca
     4681 gcaggcgttg ctcgtagatc cactggacgg ggtgctggct gtccatgagg atcggaccca
     4741 ggtagggcgc gccgctgagc ttcacgccgg aatccatgaa ctcgttgggc tctttgcgca
     4801 gcatctccgt agcgtgttcg gcaatcagac cgagcagatg gctgatcccg cggtcatccg
     4861 ggtaattgac gatgatgatt cgggtgctaa agcgctcgct tgcgggttgg cccagggtga
     4921 gggcgcacag gtcaacggcg gggacggggc gtccgtggta attgaagata ccggccacgc
     4981 ctttaggcgc ctgcggcagt tgcttgagct ccagcagcgg cacgacctcg accacgcggc
     5041 tcgcttgcag cgcgtatcgt tctttgccca attggaagag cagaaacagc atggttaaac
     5101 tgcgagcttg aaccgagcca cactggtttg cagaccgcgg gcggcgctat tcagttgctc
     5161 gatggccagg ttggattgcc gcagggattc ggcggtctgc tgcgccgctt cgctgagttg
     5221 cgtcagcgtg tcgctgatct gctgggcgcc ggtggcctgt gcgtgcatgc cttcattcac
     5281 cgtctggaac cgcggggtga gcgtttggac ctggtggata atctgcgcca gatgcgtgct
     5341 cacgtgccgg atttcctcca ctccgcggcg cgcttcctcg gaaaacttgc ccatgcccat
     5401 caccccggcg gcgacggcgg attgcatctc cttgaccatc ttctcgatgt cgtaggtcgc
     5461 gacggctgtc tgatcggcca ggcggcggat ttccagggcg accaccgcaa agcccagacc
     5521 gtactcgccg gccttttcgg cttcaatggc ggcattgagc gacaacaggt tggtctggtc
     5581 ggcaactttg gtgattgtgg tgacgaccga gttgatgttg gtcgtcttct cgctgagcac
     5641 cgagagtttg gcggtaatgg agccggacgc ttcggtgatc tggcgcatgg tagtttccat
     5701 gcgggtgagc gcggtgcggc cgctgccggc aaggtcggcc gtttgttcgg cgacctggtt
     5761 cacctcgttc atggtcttca ccagctcctt ggaggtggcg gagatttgct ttgaggtggc
     5821 gccgatctcc gccgtcgtcg cggcgatttc gtgggcggtg ctctgttgtt cccgggcggt
     5881 cgcggcaatc tcggtcgcgg tggtgttgac ctgaatgccc gaacgctgga cctggcccac
     5941 cagctcggac aggtcatctg ccagccggtt gaggccattg cccaggacgc cgaactcatc
     6001 gcgccgttgc aaagtcaggc gctcggtgaa gtccccctgg cgcatgcgtt ccagggccgc
     6061 gaccaggcga ttcagcggct tggcaacggt cgaggaatgg acgcgcccga cgatcgggat
     6121 ggccaggagg aggaggatga tgatgggggt gaggccgacc gatgcaagcc tcgcgccgcg
     6181 catcgattcg gcgtttctca cccgctcaat ctcaaaggtc aggtcagtaa acagtctgac
     6241 gcgcttttcg cgaatgtccg gatactcctt gttgtagagc ataatcgcgc tggcggagtc
     6301 ggtctccgcc gtggccagca cctggcggtg gaagccggtg agtgttcgcg acgcaaactc
     6361 gctcaggttg ctcaccgagc gtatcaggtc gggatagtca gtaaactccg attggatggt
     6421 tctcagttgc gagcccagat ccgattccgc tacgagcttg cgcttttcct cggctacgtt
     6481 cttcggatcc agcagcaagc cgcgaacggt gtcgctgata aagaccacat cgtacttgat
     6541 actgtttttg gcttcggaca actggtcgcc gcggcggacc ctggccgagc cggcgtcggc
     6601 cgcccaaagc gccaggccga cgccaaccag tagcagcgcg accagcagcc agaaggcgat
     6661 gttgagacgt tgccaaatag tcacggtctt catgggtggt tggtgtctgg ggttgaaggg
     6721 cgtgccctgt ggcctggccc cgccgacaat gcccggaagg aacggcagcg cggggcaagt
     6781 gctgagttcg cacctgactg gaccattcgt cccggtggaa tcgtcgaatt tggcatgaca
     6841 gtcatgtgca tggttttctc gcttttctgt gagcctgata attagcctgt tggccgctgg
     6901 aaaacaagaa tgaagtgaag ccgagggcgc tagcgtcatg atctaccgac ttgtctggtg
     6961 gtcgtccgca aggactgggg gttctggcgc gggcttgcgg ccacatcctg aatgatcccg
     7021 catcccgccg ggccgacaca cgacgccgac aacctgctgt cgccgacaac ctgcttgcgc
     7081 ttccagtcaa acgcttgtag ataagtgagg tgaattcttt gtattgtatt caaaggctcc
     7141 cattcggcac gcgaggggct atgcttttct taatggccgc cctggctggt tgcgaccgtg
     7201 ggcaagtcca ggagtatcgg gtggcgaagg aacaaactca ggcgcagccg agcgccatgc
     7261 cgcccggcca tcccgatact tcgggcgccg cttcccctgt gcttaaatac aaagtcccaa
     7321 cagcgtggca ggaggtcgtc ccgggtgaaa tgcgggcggc atcgttccgc gttgccgaca
     7381 agcaggcgga tgtgagcgtc atcccattgc ccggcttggc ggggagcgac cttgacaacg
     7441 tcaaccgctg gcggggccag gtcggtctgc cgggcgtgcc ggaggcggaa ttggccgcgc
     7501 tcgcgcaacc ggtcgagatc gccggccagg ccgctaagct ctatgaacag gcgggcgcaa
     7561 atgccggctc gggcgaaaag agccgcattc tcgcggccat tacgcgccgc gagggagcag
     7621 cctggttctt caagatgacc ggcgaggacg gcgtggtcgc agagcagaag ccggccttta
     7681 tcgagtttct caagtcggtg agctttacgg cggccggcgc gcaggctcaa ctgccgcctt
     7741 cccatccccc gatcgacggc gggagcatga tggcagcagc cggagcagcg ccttcttccg
     7801 gccaggccaa accgaactgg gaagtgccgt ccggctggaa ggaaatccct ggcgggcaat
     7861 tcctcgtggc caagtttgtc cttacgggcg ccgccaatgc actggcgagt gtcaacgtca
     7921 gcatgtcttc cggggacggc ggcggtatgg tggccaacgt caaccgctgg cgcaaccagc
     7981 ttggcctcgg gcccgaggcg gaagctgatc tgaccaaaga gcttcagccg ctcgacttgc
     8041 cgggcggcaa agcgacgctg gcggatatca ctggtcagga tgccaagacg gggcagaagg
     8101 cgcgcctcct ggcggtggtg gtcccgcgct cgggcgaaac ctggttttac aaactgatgg
     8161 gcgacgcgca aatcgttgaa agggaaaagg atgccttcat caagttcgtg cagaccgtga
     8221 agtattagca ccgaatgccg gatgcccatc tccctggatc aatttctgga tcgcctgatc
     8281 aaagtcttca cctcgctgcg actgacggtc gtctgcctcg cgttagggct gctgctggtc
     8341 ttctttggca cgctggcgca ggtggatttg ggcctttaca aggcccagaa tgagttcttt
     8401 cgcagcttcc tcatctactg gggacccaaa ggcgcctcgt ggaaagtccc catcttcccg
     8461 ggcggatacc tcattggggg agtgctgctg atcaacctct tcgcgtcgca gttcaagcgc
     8521 tttgtcttca cccccagcaa gattggcctc tggatggtgc atgtcggctt gattctgttg
     8581 ctgctcggcc agcttctgac cgacctgctt tcacgcgaaa gcaccctcca cctccgcgaa
     8641 ggcgaggcga ggaactattc cgagaccgag cgagatgccg agctggccgt gagcgatgtc
     8701 actgatcccg ccaccgacaa agtcgttgcc atcacccagg gcgttctggc caggcagcag
     8761 aacattcgcc ctggcgacct gccggtcatg gttcaggtga agcaattctt cgccaattca
     8821 accgtcgagg agcgggccgc tagtgccgcc cagcctcccg ccgccacgca aggcatcggc
     8881 ccccgcgcga cggtaaaaga gttgccgcgc gtgaccgaga tggaccggcg cgacgtgccc
     8941 agcgccgtag tggagatcgt cac
//
LOCUS       DATCGS010000550         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_493141, whole genome shotgun sequence.
ACCESSION   DATCGS010000550 DATCGS010000000
VERSION     DATCGS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493141"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="hemC"
                     /locus_tag="VIL39_11315"
     CDS             complement(<1..254)
                     /gene="hemC"
                     /locus_tag="VIL39_11315"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="transformation of porphobilinogen to
                     hydroxymethylbilane in porphyrin biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5482063.1"
                     /translation="MPPTPPIAIATRGSALALAQANLVLVQCRAAFPKLGFELKIIKT
                     TGDKLQTAPMAQEGTSLPKGLFTKELEVALLKHRADLAVH"
     gene            complement(417..1472)
                     /gene="hemA"
                     /locus_tag="VIL39_11320"
     CDS             complement(417..1472)
                     /gene="hemA"
                     /locus_tag="VIL39_11320"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5482064.1"
                     /translation="MLVVLGLSHHSSPVSVRERFAFAEARLPAALQSLRDSGVADEAV
                     ILSTCNRVEIYAVTQLEPPRAFAALREFLITSHDYRDPLTDEIYAWGEPQSVHHLFKV
                     ACGLDSMVLGETEILGQLKKAYDLALKNGHTGGRLNKAFQRAFNVAKHIRTETNIQRG
                     SVSVGSVAVELAEKIFSRLSDRDVMIIGAGDTSEKTARALLSRGARSILVSNRSHDKA
                     VALANELGGRAVPFENWADEFANIDIVVSSTSAPHYILDRVKLEPLMKLRRNRPLLLI
                     DIAVPRDIEPEVNFLEGVYLYNIDDLQAIAADYLKQRQGEIARCEQIIREKAEALLGH
                     PGRRESGGGVRPAFGHE"
     gene            complement(1485..2312)
                     /gene="ccsA"
                     /locus_tag="VIL39_11325"
     CDS             complement(1485..2312)
                     /gene="ccsA"
                     /locus_tag="VIL39_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5482065.1"
                     /translation="MDWLTDRHCFLLAVVVYGVSMIYSVFLWRNGFRRDNHVNYVLLL
                     FAFGLHSSAMLLRGFRLNHCPVSNLYDATLFAMWTLVAIYLVVGLWSRLRFLGAFASP
                     GLFAIGVFALMPSLDAPHGIRPELPVFLTSLHAALMSLSYGAFGLSAVAALMYLTQER
                     NLKFHKLQAIFSLMPPIQRLEAAVGGLLVSGFILLTIGLALGVFDLAHINNPHSYRGD
                     PKIVWSGVVWLLYLGLVIMRWRFAQGGRRFALSAIGSFVFVLLTFWGTNALSPLHNP"
BASE COUNT          527 a          730 c          794 g          462 t
ORIGIN      
        1 ctatgcaccg ccaggtcggc ccggtgcttg agcagcgcga cttccagttc cttggtgaac
       61 aggcctttag gcagactcgt gccttcctgc gccatcgggg cggtttggag cttgtcgccc
      121 gtggtcttga tgatcttcag ctcaaagccc agtttaggga aggcggcgcg gcactggacc
      181 aaaaccaggt tggcctgggc gagtgccaga gcgctgccgc gggtggcgat ggcgatggga
      241 ggggtggggg gcatgaaaac gtgatgcgtg attcgtgaaa cgtgaaacgt gaggcatgtc
      301 aggctggcct caactcttgg atatcatagt ggcatggttc tcatatatcc ggtcaactgt
      361 attaatctca tagtctttcc ttcacgtttc acgcatcacg tttcacgcat cacggctcac
      421 tcgtgcccga acgccggccg cacacccccg ccagattccc gccggcctgg gtgtcccagc
      481 agcgcctcag ccttttcacg aatgatttgt tcgcaccgtg cgatttcgcc ctggcgctgc
      541 ttgagatagt cggcggcgat ggcttggagg tcgtcaatgt tgtagaggta aacgccttcc
      601 aggaagttca cttccggctc aatgtcgcgc ggcacggcga tgtcaatgag cagcagcggg
      661 cggttgcgcc gcagcttcat cagcggctca agtttgacgc ggtcgagaat atagtgcggg
      721 gcggacgtgc tgctgacgac aatatcaatg ttcgcaaatt catcggccca attctcaaac
      781 ggcacggcgc ggccgcctaa ttcgttcgcc agcgcgacgg ccttgtcgtg cgagcgattc
      841 gacacgagaa tgctgcgcgc gccacgcgag agcagggcgc gggcggtttt ctcgctggtg
      901 tcgcccgcgc cgatgatcat cacgtcgcgg tcgctcagcc ggctgaagat cttctcggcc
      961 aattccactg ccaccgaccc cacggagacg ctgccgcgct ggatattggt ttccgtgcgg
     1021 atgtgcttgg cgacgttaaa cgcccgctga aatgccttgt tcaaccggcc gccggtatgg
     1081 ccgttcttga gggcgaggtc gtaggccttc ttgagctggc cgaggatttc tgtctcgccc
     1141 aggaccatcg agtccaggcc gcacgcgact ttgaagagat gatgcacgct ttgcggctcg
     1201 ccccaggcat agatctcgtc tgtcagcgga tcgcggtagt cgtgggaggt gattagaaac
     1261 tcccggagcg cggcaaacgc tctgggcggc tccaactgtg tgacggcgta gatttcgacg
     1321 cggttgcagg tggacagaat caccgcctcg tcggcgaccc ccgagtcgcg gagtgattgg
     1381 agcgcagccg gcaagcgcgc ctcggcaaaa gcaaagcgct cccgcacgga gaccggcgag
     1441 gaatgatggc ttagaccgag gacgacaagc atgctagctc caaatcatgg attgtgaagc
     1501 ggagacaagg cgttggtgcc ccagaacgtc agcaggacga agacgaagct gccgatggcg
     1561 ctcagggcaa accggcgtcc gccttgcgcg aatctccagc gcatgatcac caggcccagg
     1621 tagagcagcc agactacgcc ggaccaaaca atcttcgggt cgccgcggta ggaatggggg
     1681 ttgttgatgt gggcgaggtc aaacacgccc agcgccaaac cgatcgtcag caggatgaag
     1741 ccactgacca gcagcccacc caccgccgct tcgagacgtt ggatgggcgg catgagcgag
     1801 aagatggcct gcagcttgtg aaacttcagg ttgcgctctt gggtgagata catgagggcc
     1861 gccacggcgc tgaggccgaa tgccccgtaa gagagggaca tcagcgcggc atgcaggctg
     1921 gtcaagaaaa ccggcagttc gggccggatg ccgtgcggcg catcaaggct gggcatcaac
     1981 gcgaagacgc cgatagcaaa cagacccggc gaagcaaagg cgccgagaaa ccgcagccgc
     2041 gaccatagcc ccaccaccag atagatcgcc acgagagtcc acatggcgaa aagggtggcg
     2101 tcgtagaggt tgctcaccgg gcaatggttc aggcgaaagc cccgcagcag cattgccgag
     2161 ctgtgaaggc caaaggcaaa cagcagcagc acgtagttga catgattgtc ccggcgaaac
     2221 ccgtttcgcc aaaggaacac cgagtaaatc atgctcacac cgtaaacgac tacggccagc
     2281 aggaaacagt gtcggtcagt tagccaatcc acggtaacag attaagccgt caggcggcgc
     2341 gtgcaagtgg caattgctgc ctcggcggag ggagggagca ccaaatccct gtctgcggtg
     2401 acagccggca ccgccaatcc tggggactgc gccgtcaaag gaacaccctc gtgcctgccg
     2461 agtttcaagc tcgtttcaag ctcgtttgag gctcgttttg aagcttccgc att
//
LOCUS       DATCGS010000551         3704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_591751, whole genome shotgun sequence.
ACCESSION   DATCGS010000551 DATCGS010000000
VERSION     DATCGS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591751"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1376)
                     /locus_tag="VIL39_11330"
     CDS             complement(12..1376)
                     /locus_tag="VIL39_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5482066.1"
                     /translation="MPNTLSARPITRREFTQTLAALSTTAALSSFRVLGANDRVRLGF
                     IGVGNRGDQVLDAFLKQSDAEVVAVCDIYEPYLDFAAKKIGTSPKRFKDYRALLGLKD
                     VDAVVISTPDHWHALQTIHACQAGKDIYVEKPVSLRVAEGRAMVEAVKHHGRVCQSGL
                     IRRSSAMIREMAEFVRGGSLGKVTAVRCFHTQNEWPKGIGNPPDSEPPPGLDWEAWLG
                     PAPKVPYNRNRGLYQFRWFYDYSGGQITNMGVHYLDVIHWALGRSAPLAVTAMGGNYA
                     VSDNREIPDTMEALWRYPGDTLVTFTQFNANGANASAKPCEIEFRGTKGTLYLRSNGW
                     EVVPENITPNEFPQRSPIDRNLERGWRTGEAPMIKPKIASGRTDNADHARNFLDCIKS
                     RAACNSDLESAHRSTSTTLIANIAMQTKAYLEWDAKTEKFTNHTAANKLLRYDYRAPL
                     KSPA"
     gene            complement(1411..1932)
                     /locus_tag="VIL39_11335"
     CDS             complement(1411..1932)
                     /locus_tag="VIL39_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482067.1"
                     /translation="MKLSNTNRRPGKPEALGQSARTGLHFMPRFVSVLCAGALCGFLV
                     SCGSKTDTPLAPRASETNAAPAAAAASPELAKLVGKWERPDGGYILEIKSVDASGKLD
                     AGYFNPDPINVARAAAMRDKGTNKVFIELRDANYPGCTYSLTYDPQSDQLYGQYYQAA
                     MQQTFDVTFARVK"
     gene            complement(1913..2260)
                     /locus_tag="VIL39_11340"
     CDS             complement(1913..2260)
                     /locus_tag="VIL39_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482068.1"
                     /translation="MTKRPTMRQLLAMIPNVPCLYGHEVNSTYYGIKKLKGKEKDICV
                     GLGALPDTNTKAGGHANRNAGFIRQPQRGSPVCRMNPAFRWWCQDTPSVWVLRRSAAG
                     TILPERYHETLQY"
     gene            2352..2427
                     /locus_tag="VIL39_11345"
     tRNA            2352..2427
                     /locus_tag="VIL39_11345"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2385..2387,aa:Val,seq:tac)
     gene            2578..3297
                     /locus_tag="VIL39_11350"
     CDS             2578..3297
                     /locus_tag="VIL39_11350"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY5482069.1"
                     /translation="MKTTALASAALATATTLRTALAQPTPGPTAAAGATGPFTLPPLP
                     YAFDALEPHIDALTMQIHHDKHHAAYVANLNKAVAEFPELGKKRVEDLLRDLNAMPEK
                     IRTAVRNQGGGHYNHTLFWQMMKKDGGGQPSGELLKGVEKRFGSFPAFKDEFTKAALG
                     QFGSGWAWLVLDPDKHLGIEPTPNQDSPISQNKQPLLTVDVWEHAYYLKYQNRRPEYV
                     AAFFQVIDWDFVSERYKQALS"
     gene            complement(3313..>3704)
                     /locus_tag="VIL39_11355"
     CDS             complement(3313..>3704)
                     /locus_tag="VIL39_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482070.1"
                     /translation="HLSPGSPCPGRVLRAAVLSFCVVGSGSAPAADWVQAGLNTNQPI
                     WGLRGGPLWHYGGSKVTQYWKKPRGTARDDLHVAVNARYTYWQSRRPIPGGVAFENFE
                     LRERFYDGQPFVFGITRETPDQLRGAK"
BASE COUNT          699 a         1116 c         1158 g          731 t
ORIGIN      
        1 tctgtccgcg gttacgccgg agacttgagc ggggcgcggt aatcgtagcg caggagcttg
       61 ttggccgcgg tgtggttggt gaacttctcc gtcttcgcgt cccattccag gtaagccttg
      121 gtctgcatcg cgatgttggc aatgagcgtg gtgctggtgc tgcggtgggc gctttcgagg
      181 tcggagttgc acgccgcgcg gctcttgatg cagtcgagga agttgcgggc gtggtcggcg
      241 ttgtcggtcc gcccgctggc tatctttggc ttgatcatcg gcgcttcgcc cgtgcgccag
      301 ccgcgctcga ggttgcgatc aatggggctg cgttgcggga actcgttcgg cgtgatgttc
      361 tccggcacca cttcccagcc gttgcttctc aggtaaagcg tgcccttggt gccgcgaaat
      421 tcgatctcgc acggcttggc cgatgcgttg gcgccattgg cgttgaactg ggtgaacgtg
      481 accagcgtat cgcccgggta acgccacagc gcttccatcg tgtccggaat ctcacggttg
      541 tccgagaccg cgtaattgcc gcccatggcg gtcacagcca acggcgcgct ccgcccaagc
      601 gcccagtgaa tcacatcgag gtaatggacc cccatgttcg tgatctggcc gccactgtag
      661 tcatagaacc agcggaattg atagagcccg cggttccggt tgtagggcac cttcggcgcc
      721 ggccccagcc acgcctccca gtccagtccc ggcggcggct cgctgtcggg cgggttccca
      781 atccccttcg gccactcgtt ctgggtgtgg aagcagcgca cggcggtgac cttgcccagc
      841 gagccaccgc gcacaaactc cgccatctcc cggatcatgg ccgaggaacg acggattagg
      901 ccggattggc agacgcgccc gtggtgcttc accgcctcca ccatcgcgcg cccttccgcg
      961 acgcgcagtg aaacgggctt ctcaacatag atatccttcc ccgcctgaca ggcgtggatg
     1021 gtttgcagcg cgtgccaatg gtcgggcgtg gagatgacca ccgcgtccac gtccttcagt
     1081 ccgagcagcg cgcggtaatc tttgaaccgc ttggggctag tgccgatctt cttcgccgca
     1141 aagtccaggt aaggctcgta gatgtcgcag acggccacaa cctccgcgtc gctctgcttg
     1201 aggaaggcgt ccaggacctg gtcaccgcga ttgcccacgc cgataaagcc cagccgcacg
     1261 cggtcattgg cgcccaggac gcggaaagac gagagtgcag cggtggtgct gagggcagcg
     1321 agtgtctggg tgaattcccg gcgggtgatt ggcctggcag acaatgtgtt tggcataatg
     1381 tttgatttaa tcagtgggag gccgacgaat tcacttcacg cgggcaaatg tgacgtcgaa
     1441 ggtttgctgc attgcggcct ggtaatactg cccgtacaac tgatcgctct gaggatcgta
     1501 agtaagacta taggtgcagc cgggataatt ggcgtcgcgc aattcgatga agaccttgtt
     1561 cgtcccctta tcgcgcatgg cggcggcccg cgcgacattg atcgggtcgg gattgaaata
     1621 gccggcgtcc agcttgccgc tggcatccac gctcttgatt tcgaggatgt agccgccgtc
     1681 gggccgctcc catttgccca ccagcttggc cagctccggc gaagccgcgg cggcagccgg
     1741 tgccgcattg gtctcggatg ctcgcggcgc caggggtgta tccgtcttgc tgccacagga
     1801 caccaggaaa ccgcaaagcg cgcccgcgca gagaactgat acgaatcgcg gcatgaaatg
     1861 aagtcccgtg cgggctgatt gaccgagagc ttccggcttg cccggtctgc gattagtatt
     1921 ggagagtttc atgatatcgc tccggcagaa tagtccccgc agccgagcgc ctcaataccc
     1981 aaaccgaggg tgtatcctga caccaccacc ggaatgccgg attcatccgg cagaccggcg
     2041 aaccgcgctg gggctgccgg atgaatccgg cgttccgatt ggcatggcca cccgccttgg
     2101 tgttggtgtc gggaagcgcc cccaaaccga cacaaatgtc tttctccttg cccttaagct
     2161 tctttattcc atagtacgtg ctgttgactt catgcccgta gagacacgga acattcggaa
     2221 tcatggccag gagttgcctc atggttgggc gcttcgtcac ggtgcgaatg ttaggggatg
     2281 gacaaacagg gggggccaac aagaaagcag cttttcaagc atcgtcataa gtgcttgtaa
     2341 ataggcttga tgggcgcttg gcgcagtggt tagcgcgtct cgtttacacc gagtaggtcg
     2401 ggggttcgag tccctcagcg cccaccatct tttgaatggt gatcctggag accccaactt
     2461 gcgctggtgc cacagtggtg cgaatagtgg atcaggcctc ggcggaagac gccccggcac
     2521 gttgccaggc aaccatgaga taaaggaact ctgattatga tgactcgcag gcaggctttg
     2581 aaaacaaccg cgctggcgtc ggcggctttg gccacggcca caactctgcg cactgcactg
     2641 gctcagccca cgcccgggcc gacagcagcc gccggggcga caggcccatt cactctccct
     2701 cctctgcctt atgcgtttga cgcgctggag ccgcatatcg acgccctcac catgcagatt
     2761 caccacgaca agcaccacgc ggcttatgtg gcgaatttga acaaagcggt ggccgaattc
     2821 cccgagctgg ggaagaagcg ggtcgaggac ttgctgagag acctgaacgc tatgccggaa
     2881 aagatccgca ccgcagttcg caaccaaggc ggggggcact acaaccacac gctcttctgg
     2941 cagatgatga aaaaggacgg aggcggccag ccttcgggcg agcttctgaa gggggtcgag
     3001 aagcggtttg gcagcttccc cgccttcaag gacgagttca cgaaagccgc tttgggccag
     3061 ttcgggagcg gttgggcctg gctggtcctc gatccggaca agcacctggg catcgaaccg
     3121 acgcccaatc aggattcgcc gatcagccag aacaagcaac ccctgctcac ggtggatgtt
     3181 tgggagcatg cctactacct caagtatcaa aatcgccggc cagaatacgt ggcggcgttc
     3241 ttccaggtga ttgattggga cttcgtcagc gagcgctaca agcaggctct cagttgatag
     3301 aagcggatag cactatttcg ctccccgtag ctggtctggt gtctcgcgcg tgataccgaa
     3361 gacgaagggc tgcccgtcgt agaagcgttc acgcagctcg aagttctcaa acgcgacccc
     3421 gccgggtatg ggccggcgcg attgccagta ggtgtagcgg gcgttgacgg cgacgtgcaa
     3481 atcgtcccgc gccgtcccgc gcggcttctt ccagtactga gtcaccttgg agcctccgta
     3541 atgccacagc ggtccaccgc gaaggcccca aatcggctgg ttcgtgttca gcccggcctg
     3601 cacccaatct gccgccggtg cgctcccact accgacgaca cagaaagaga ggacggcagc
     3661 gcgaaggacc cggcccgggc aaggcgaccc aggcgaaagg tgtc
//
LOCUS       DATCGS010000552         5857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_641045, whole genome shotgun sequence.
ACCESSION   DATCGS010000552 DATCGS010000000
VERSION     DATCGS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641045"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIL39_11360"
     CDS             complement(<1..340)
                     /locus_tag="VIL39_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482071.1"
                     /translation="MLNRRTFLRSTSLALGTCGLARYGVVEGWAGAAPAVKEAVLTAA
                     DLPRGNAPKPVPFPHFPDRLHAFVWRNWQLVPARRMAEVVGASAGDIVRLGRSMGLSG
                     PPRITVEQQRR"
     gene            complement(540..1487)
                     /locus_tag="VIL39_11365"
     CDS             complement(540..1487)
                     /locus_tag="VIL39_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5482072.1"
                     /translation="MPFDRDKMKAAAAALAGEGVFIGTSSWKYPGWRGQLYHEDRYIW
                     HGKFSEARFERLCLAEYAEVFKTVCVDAAYYKFPDHRFLEQLVAQVPAGFLFALKVTD
                     QITIKRFANLPRFGWRAGAANEHFLNAGLFESAFLRPCREFQANIGLLIFEFSHFSPR
                     EFERGREFVEALDGFLAKLPRGWRYGVELRNRSFLHPEYFAALARHGAAHVFNSWQDM
                     MPVNEQLALPESRTSPAFFSARLLLKPGRKYEEAVKLFSPYDRIKDPYPEGRAAGASL
                     IQEARSRGGAKQGFIYVNNRLEGNALETIAALVEQAGAG"
     gene            complement(1593..2795)
                     /locus_tag="VIL39_11370"
     CDS             complement(1593..2795)
                     /locus_tag="VIL39_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482073.1"
                     /translation="MAFRIHDSVVRGEIDNRVKGIVRGQIWIEGRAEPVVLELKGNAW
                     PDLAGCRLTFTNPQQRVPHAHLDSLHPLQHGSIGDLTASRKVRVFDVPLPDALDMIHR
                     KEKPSEHMANCLYLEWFSEANGRVVIESADYELTISAPEWRLTPEEDAERAKQADTGM
                     ADFMGKLSEAIEQHQSGQKDPEEPWDEHDYEKFLKESDARTDKYMELLDKYGDSDEAE
                     AKIAREMGWERELTPEEAELEQQRIEEMNAACEAALNEPEPEPEPHREGIDWIRTEDG
                     DLCHPLEHRCSETAIRYWKQVDELGLDKMGDKDLEQFIFEFQTTSAKLAGALGGVARG
                     TGFADASFTVACLKRALDHLHKSQAGLEATAAKKLLPETLIAEARKELFEIREGILRL
                     MDEFRGRA"
     gene            complement(2852..4408)
                     /locus_tag="VIL39_11375"
     CDS             complement(2852..4408)
                     /locus_tag="VIL39_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5482074.1"
                     /translation="MIFHSIRWRLQAWYGLILVAVLAGFGLTAYHVARDNQLRRIDQD
                     LDLRIKTLFRPQSSGGPPERFLDQPPDGPRDLPLEPRSGQRRNELPGEPRNDRGFGFP
                     DFLFTVRDAVQRGGTLDPSQTNTFYYVLWQEDGSILARSPGAPVSVPAPKHVGTAGSQ
                     AFRQRDAFKKGSLANVGPDVPSEARTRGQMREMFRFLPRGQCVLVGRSLAPDLAAMRR
                     LALWLGAAGAAVLVLGLAGGWWVATRAIQPIEAISATAVKIAGGDLSQRINAADTDSE
                     LGRLAGVLNSTFARLEAAFTQQARFTSDASHELRTPVSVILSQTQTALSRQRPGPEYR
                     EALEACQRAARRMKTLTESLLELARLDAGQEAMKQERFDLLRVVGECLEMVRPLAAER
                     GIKIHCDVAAIECLGDAGRVGQVVTNLLTNAIAFNRDQGEVRLAARIEENAVLLTVSD
                     TGQGIPEEDVPHLFERFYRADKSRSRIQGRNGLGLAICKAIVDAHGGSLEVSSQLEVG
                     STFTLKLPLK"
     gene            complement(4405..5064)
                     /locus_tag="VIL39_11380"
     CDS             complement(4405..5064)
                     /locus_tag="VIL39_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5482075.1"
                     /translation="MRVLAVEDEPDLLSSLIKALREDGYAVDGAPDGEEGLYKAESYD
                     YDAIVLDIMLTGIDGWELLRRLRKTKKTPVLMLTARDAVRDRVRGLDTGADDYLVKPF
                     ELAELLARLRALIRRSASQAQSRLQIGDVVIDTAARTVMRRGETIALTAREYSLLEFL
                     ALHRGKLVTRTTLYDHLFDENDSTLSNLLDVHVFNIRKKLGHDFITTRRGHGYSIESP
                     P"
     gene            complement(5310..>5857)
                     /locus_tag="VIL39_11385"
     CDS             complement(5310..>5857)
                     /locus_tag="VIL39_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEY5482076.1"
                     /translation="RRDTWANNVEDWESSDDNTNVVYLTDSLLGPYANGAARVYKCPS
                     DREPAPNGPRTRSMSMNAMVGNPGELTNRFNPLYVQFYKLAGVPNPSGIFVFLDEQAD
                     TLNDGFFVNRLDGYAWGNVPGSYHNGAVNLAYADGHGESHRWLVPDTVRPVLRTRIDM
                     FPAAPPTDFEWLKARTSLKKP"
BASE COUNT         1061 a         1868 c         1838 g         1090 t
ORIGIN      
        1 cacggcgttg ctgctcgacc gtaatccgcg gcggccccga cagccccatg ctccggccca
       61 ggcgcactat gtccccggcg ctggccccga cgacttcggc catccggcgg gcgggcacca
      121 gttgccaatt gcgccacacg aaagcgtgca ggcgatccgg aaagtgcggg aacggcaccg
      181 gcttcggggc gttgccccgc ggcaggtcgg ccgcggtcag cacggcttcc ttcacggccg
      241 gagccgctcc ggcccagcct tccaccacgc catatcgcgc cagcccgcag gttcccaaag
      301 ccagtgaagt agatctgagg aaggttctac gattcagcat cccgcccgtt cgttcttgat
      361 ttgaggctat agtggacatg tgtgaccgcg tgataaatca tcctcctgcg aaaggcaaga
      421 cggctgatag ggaaactcaa aaagtggcag gcccagtcgc ctccggtggt ggtactcgcc
      481 aaatgcgccc ctggtcggcg cacccatcgc cggaacagtt gacatgccgg caggtctcgc
      541 taacccgccc ctgcctgttc taccagcgcg gcaattgtct ccagggcgtt gccctcaagc
      601 cggttgttca cgtaaatgaa gccctgcttt gctccgccac gactgcgagc ttcctggatc
      661 aggctggcgc cagccgcccg gccttccggg tagggatctt tgatgcggtc gtaagggctg
      721 aacagcttca ccgcctcttc gtatttgcgg cccggcttga gcagcagacg cgcgctgaag
      781 aacgcggggc tggtgcggct ctcgggcagg gcgagttgct cattcacggg catcatgtcc
      841 tgccaactgt tgaagacgtg ggcggcgccg tggcgcgcca gcgccgcaaa atactccgga
      901 tgcagaaagc tccggttccg caactcgacg ccgtagcgcc agccccgggg cagcttcgcg
      961 aggaacccat ccagcgcttc gacaaactcg cgcccccgct cgaactcgcg cggcgagaag
     1021 tgcgagaact cgaagatcaa caggccaatg ttcgcctgga actcccggca agggcggagg
     1081 aacgccgact caaacagtcc ggcattcagg aaatgctcat tggcggcgcc ggcgcgccag
     1141 ccgaaccgcg gcaggttggc aaagcgcttg atcgtgatct ggtccgtcac cttgagcgcg
     1201 aagaggaagc ccgccggcac ctgggcgaca agctgttcga gaaaacggtg gtcggggaac
     1261 ttgtagtagg ccgcatccac gcacaccgtc ttgaacacct ccgcatactc cgccaggcaa
     1321 agccgctcga agcgcgcctc ggagaacttg ccgtgccaga tgtagcggtc ctcgtgataa
     1381 agctggcccc gccagccggg atacttccag gaagaggtgc cgataaagac gccttccccg
     1441 gccagggcgg cggcggccgc tttcatcttg tcgcggtcaa aaggcatgcg cttcggttaa
     1501 tgtgcagccc ccggtgctgc ttttcaataa agcatcactt ccgcggcagc ccgattccaa
     1561 ctgagttccc cgctggctga caggcgtttg gctcaggcgc gcccacggaa ttcgtccatc
     1621 aagcgaagga tgccttcgcg gatttcaaac aactccttcc gcgcctcagc aatcaatgtc
     1681 tcgggcagaa gcttcttcgc ggcggtggct tccaaacccg cctgagactt gtgcaaatga
     1741 tccagcgcgc gcttcagaca ggcgaccgtg aacgaggcgt cggcaaaacc cgttccccgg
     1801 gcaacgccac ccagcgctcc ggcgagcttg gcgctggtgg tctggaattc aaagatgaac
     1861 tgctccaggt ccttgtcgcc cattttgtcc aggcccagtt catccacttg cttccaatac
     1921 ctgattgcgg tctcagagca gcgatgttcc agtgggtggc acaggtcgcc gtcctcggtg
     1981 cgaatccagt caatgccttc gcgatgcggc tcgggttccg gctcgggctc gttcaacgcc
     2041 gcctcacacg ccgcattcat ttcctcgata cgctgctgtt ccagctcggc ttcttccggc
     2101 gtcaattcgc gctcccagcc catctcccgg gcgatcttcg cctcggcttc atccgagtcg
     2161 ccgtatttat ccagcagctc catgtatttg tccgtgcgcg catcggattc cttaaggaac
     2221 ttctcgtaat cgtgctcatc ccaaggctcc tcggggtctt tctgtccgct ctgatgctgc
     2281 tcgatggctt cgctgagctt gcccatgaaa tcagccatgc ccgtgtcggc ttgtttggcg
     2341 cgttccgcgt cctcctccgg cgtcagacgc cattcgggcg cggagatcgt cagctcataa
     2401 tccgcgctct cgatcaccac gcgcccattc gcctcgctga accattcgag ataaagacag
     2461 ttggccatgt gctcggacgg cttctccttt cggtgaatca tatcgagcgc gtccggcagc
     2521 ggcacgtcaa acacgcgcac ctttcgtgac gcggtgaggt cgccgatgct gccatgctga
     2581 aggggatgga gcgaatcgag atgggcatgg ggaacgcgct gctgcggatt agtgaacgtg
     2641 agccggcagc cggccagatc tggccaggcg ttgcccttga gttcgagcac caccggttcg
     2701 gcccggccct cgatccagat ttggccgcgc acaattcctt tgacgcggtt gtcaatctcg
     2761 ccccgcacta cgctgtcatg aatgcgaaaa gccatgcgcc aacaatgcca gacttcgcca
     2821 gaacgacaag acttcccgaa ggggccgcgt cttacttcag cggcagctta agcgtgaaag
     2881 tgctgccgac ttcgagctgg ctggaaactt cgaggctgcc gccgtgggca tccacaatcg
     2941 ctttgcagat cgccaggccg aggccgttgc ggccctggat gcgcgagcgg gacttgtctg
     3001 cccgatagaa ccgctcgaac aagtgcggca catcttcctc ggggatgccc tggccggtgt
     3061 cgctaaccgt caggaggacc gcattctcct cgattcgggc agcgaggcga acctccccct
     3121 ggtcgcggtt gaaggcgatg gcgttggtga ggaggttcgt aacgacctgg ccgacgcgtc
     3181 cggcgtcgcc gaggcattca atcgcggcca cgtcgcagtg gattttaatc ccgcgctcgg
     3241 cggccagcgg gcggaccatc tcgaggcact cccccacgac ccgaagcaag tcgaagcgct
     3301 cctgtttcat ggcctcttgc ccggcgtcga ggcgcgccag ttcgagcagg gattcggtga
     3361 gggtcttcat gcggcgggcg gcgcgctggc aggcttccag ggcttcgcga tattcggggc
     3421 cgggccgttg gcgcgaaagg gcggtctggg tctgagagag gatcaccgag acgggtgtgc
     3481 ggagttcgtg cgaggcatcc gaggtaaacc gggcctgctg ggtgaacgcg gcttccagac
     3541 gggcaaaggt cgagttcagc acgccggcca gccggcccag ttcgctgtcg gtgtcggcgg
     3601 cgttaatgcg ctgggaaagg tccccgccgg cgatcttcac cgcggtggcg ctaatcgctt
     3661 caatgggctg gatggcgcgg gtggccaccc accaaccgcc cgccaggccg agcaccagca
     3721 cggcggcccc ggccgcccca agccacaggg ccagccgccg catggcggcc aggtcgggcg
     3781 ccagcgagcg gcccaccagg acgcactggc cgcgcggcag gaagcgaaac atctctcgca
     3841 tttggccccg ggtgcgcgcc tcagacggca catccggacc gacgttggca agcgaacctt
     3901 ttttaaatgc gtcgcgctgc cggaacgctt gcgacccagc ggtaccaaca tgcttgggag
     3961 cggggacact gaccggcgcc ccgggtgagc gcgccaggat cgagccatcc tcctgccaca
     4021 aaacataata gaaggtattg gtctggctgg gatcgagagt gcctccccgc tgcaccgcgt
     4081 cgcggactgt gaagaggaag tccgggaaac cgaagccgcg gtcgttgcgc ggctcaccgg
     4141 gcagctcatt gcgccgttgc ccggaccgcg gctcaagcgg taggtcgcgc ggcccgtcag
     4201 gcggctggtc caggaaacgc tcgggcggcc cccccgacga ctgggggcgg aacagagtct
     4261 ttatccgcag gtccaggtcc tgatcaatcc gccgcaactg gttatcgcgg gcgacatgat
     4321 aggcggtaag cccgaagcca gcgagcaccg cgaccagaat cagcccatac cacgcctgga
     4381 gccgccagcg gatggagtgg aagatcatgg cggagactcg atgctgtagc cgtggccgcg
     4441 ccgcgtcgtg atgaagtcgt gcccgagctt cttgcggata ttaaagacat gcacatcgag
     4501 gagattggac agggtgctgt cgttctcatc gaagaggtgg tcgtaaagcg tcgtgcgggt
     4561 caccagcttg ccgcggtgca gcgcgaggaa ttccagcagg gagtattcgc gggcggtgag
     4621 cgcaatcgtc tcgccccggc gcattacggt gcgcgcggcg gtatcaatga ccacgtcccc
     4681 aatctgcagc cgggattggg cctggctggc ggagcggcga atgagggcgc gcaaccgggc
     4741 gaggagttcc gccaactcga agggcttgac gaggtaatcg tccgcgccgg tgtccaggcc
     4801 ccgcacgcgg tcgcgcaccg catcgcgcgc ggtgagcatc aacacggggg tcttcttggt
     4861 cttgcgcaag cgccgcagca attcccagcc gtcaatgccc gtcagcatta tgtccagcac
     4921 aatggcgtcg tagtcatagc tctccgcttt gtacagcccc tcctcaccgt ccggggcgcc
     4981 gtccacagcg tagccgtcct cccgcagggc cttgatcagg ctgcttagga gatcgggttc
     5041 gtcttcaact gccagcactc tcatacgtgt cgcctcggtt ctttatgcgc tcagtttgca
     5101 tggtaatcaa cgcatttcac cctgccacaa acaaccttaa gggaagatta agggccgcgt
     5161 tcaggctcca gccgccacga gcacgcgctt gagcctgttt aaaaaattgg tttggagttc
     5221 cgcctttagg cggtacggcg cggaaaccgc ctaaaggcgg aactccaaac ctgccgcagc
     5281 gggtctcaat gatctttttt aaacaggctc taaggcttct tgaggctggt tcttgccttg
     5341 agccactcga agtcggtggg tggcgcagcc ggaaacatgt ctatcctggt tcgcaggacg
     5401 gggcggacgg tgtcgggcac cagccagcgg tgggactctc cgtggccgtc ggcatacgcg
     5461 aggttcacgg ctccattgtg gtacgacccc ggcacattgc cccaggcata accatccagg
     5521 cggttgacaa agaacccatc gttaagggta tcggcctgtt catccaggaa gacgaaaatg
     5581 cccgagggat tcggcacccc ggccagcttg tagaactgga cgtagagggg gttgaaccgg
     5641 ttggtcagct cgccgggatt gcccaccatg gcgttcatgg acatgctgcg ggtgcgcggg
     5701 ccattggggg ccggctcccg gtctgacgga catttgtaaa ccctggcggc gccgttggca
     5761 taaggcccga gcagcgagtc ggtgagataa accacgttgg tgttatcgtc gctgctttcc
     5821 caatcctcga cattgttggc ccaggtgtcg cggcggg
//
LOCUS       DATCGS010000553         6184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_641157, whole genome shotgun sequence.
ACCESSION   DATCGS010000553 DATCGS010000000
VERSION     DATCGS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641157"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VIL39_11390"
     CDS             complement(<1..1340)
                     /locus_tag="VIL39_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5482077.1"
                     /translation="MTAPSFSRIALLGIALVLTGCGKPAADKPGASHPLPSSPLIAKG
                     EPGRHGGRLVIAATASPKTFNPLFALDSASDGIIRLLFASLVNLDWATQQPGPGLAES
                     WSVAPDQRTWTFKLRQGVRWSDGEPLTADDVVFTWTDIMYNREFNQGTFDLFHIGGKA
                     FAVSKINDFTVQVVTPEVFAPFVEFFGGVPILPKHVLEAAVKAKVFPVAYGVNSKPNR
                     IVGCGPYRLKEFRLGQFTLLERNPEYWVADRQGGRLPCIDEVMFTVHGGAGTEVSFFL
                     NGKSDVCEAVRPQNYAQLKQASAGGAFQLVEMGVGAERDFLWFNQNTGTNAMGQPIVN
                     PARLKWFRNKKFRQAFSCAIDRDRLVREVYGGHAQPASGFISTENQKWNNPDIPHYGF
                     DPARARALLAEMGIQDRSGDGVMKDADGNPIEVLFYSNAGNPLREKAAALIQED"
     gene            1938..3170
                     /locus_tag="VIL39_11395"
     CDS             1938..3170
                     /locus_tag="VIL39_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5482078.1"
                     /translation="MASGPLVAVVVVLIFAGASFFFALAETALFSLSKWQVRQLAERA
                     PGAGGIVARLLAEPQDLLATMVLGNTFASAAMLAVALWMAIHGRWPLAATVVGLLALI
                     LIGCEVLPKTLAVRRAEQWSLRVARSLSFVVQFSLPLCRVAQKVNTAILRVVMPATGQ
                     PHITLTDADYQELLEMAYQQGTLAQSEKEIILQIISLDRRTAKEVMKPRSRMAAISDD
                     LSIEDMIAAACKHKHRRLPIYDETPDTIVGILNTRALLLDPQIDLADAIEFPSFVPET
                     MNLLQLLKSLQRQQRGLAIVLDEFGGTAGIVTMEDILEELIGKIHGEAEVEGFVMEKL
                     GPGRWRVAGTLRLDDFRREYPVLGDVPEVETMGGLLTSLLDVVPAKGEAATFCGLKLT
                     AQVADERRVRELLVEAVK"
     gene            3172..4161
                     /locus_tag="VIL39_11400"
     CDS             3172..4161
                     /locus_tag="VIL39_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5482079.1"
                     /translation="MSRDSAVWVVFVLCLVVSFVLSGMEAGVFTLSRLRIRQQMRAGQ
                     ASAKVLHDYLEHPENFLWTILVGNTVANFLILGWLVAQLHEALGAYRVWFVVVFSVAV
                     FLFYTFFDLLPKMLFRIYPNRFCVLLARPFRLIHWVLRPLVALVETFSGALVRWRGGK
                     AFTPHLFGNREELRLVMQESAQGFTSEERTMINRVLDLQTLTVRQAMKPLEQAVAITA
                     QTPVSAALALCRERKFTRLPVWENRDGQQRIVGLLALNTLLYQPALDTTKPVGEYVKP
                     ALFLDEDLRLEVALRRLQRSGQRLAIVLGRDRREIGILTLQDLLKGIFGEVSL"
     gene            4164..5489
                     /locus_tag="VIL39_11405"
     CDS             4164..5489
                     /locus_tag="VIL39_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5482080.1"
                     /translation="MDWDVLFSIILRVLGVVVLVLLNAFFVSCEFSLVKVRDTQLSPL
                     VHRGHRRARVADYILRRLDAFLSAAQLGITLTSLGLGWIGEPVFAVLLGPVFGLLNVD
                     SPEMQKLLAFVIGFSVITFLHISAGEQAPKWLAIQKPLATALWISYPLLWFYRASYPF
                     VVVLNWSSQWLLRQAGIKLGDEAGRAHSEEELRLLLATSQKHSGATVLGRDIVLNALD
                     LRRRVAREVMRPRQEIVALDTEASIAECLDVAEKTRYSRFPLCEGGDLDRTLGVVHIK
                     DLYAMRIRARSGADLLPAVRKLIYVPESARLEKLLQFFLERKLHLAIVVDEFGGTMGM
                     VTLENILEELVGQIQDEFDQEKPLLTRTGENTWEVAGTLALHELEEVVGEPLQAEGIT
                     TVSGWVTQRLGGFPKPGDVLAIGPCELRVEEMAGMRVARLKLTKKQPEV"
     gene            complement(5498..>6184)
                     /locus_tag="VIL39_11410"
     CDS             complement(5498..>6184)
                     /locus_tag="VIL39_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEY5482081.1"
                     /translation="RWPDGLLVVLTTAIVLASLTRQLPGQNVMLASIIIAFIAGAVQT
                     LGALTAIPFGPYVYTERIGQQLFYPLPWAVPLMWIAVILASRGVARLTLRPWRKTQNY
                     GFRLIGLTVLLVVLLDVGLEPFATSVKHYWFWNPTKLKFDWYSTPCVNFLGWAVTALL
                     ILAFATPSLINKKPMPQPPPDYSSLVVWLLLNFLFATGAAVHHLWPAVGLISLGSLVV
                     TVFAIRGARW"
BASE COUNT         1134 a         1772 c         2083 g         1195 t
ORIGIN      
        1 atgtcctcct gaatcagggc ggccgccttt tcgcgcaggg gattgccggc attcgaatag
       61 aacaggacct caatcgggtt gccgtcggca tccttcatca cgccatcgcc gctgcgatcc
      121 tggatgccca tctccgcgag cagggcccga gcgcgggcag gatcgaagcc ataatgcggg
      181 atatccgggt tgttccactt ctggttctcc gtcgagatga agcccgaggc gggctgcgcg
      241 tggccgccgt aaacctcgcg caccagtcgg tcccggtcaa tagcgcacga gaaggcctgg
      301 cggaatttct tgttccggaa ccacttcagc ctggccgggt tgacgatggg ctggcccata
      361 gcgttggtcc cggtgttttg gttgaaccag aggaaatccc gctcggcgcc gacccccatt
      421 tcgacgagtt ggaaagctcc gccggcggaa gcctgcttga gctgcgcata gttctgcggg
      481 cgcactgcct cacagacgtc gctcttgccg ttaaggaaga agctcacttc agtccccgcg
      541 ccgccatgca cggtaaacat tacctcatca atgcaaggca aacggccgcc ttgccggtcg
      601 gccacccaat actcaggatt acgttccagc aaggtgaatt ggccgaggcg gaattccttc
      661 aggcgatacg gcccgcagcc aacaatccgg ttcggcttcg aattgacgcc gtaggcgacg
      721 gggaagactt ttgcttttac cgcggcctcc aggacatgtt tgggcaggat tggcacgccg
      781 ccaaagaatt ccacgaacgg cgcaaacacc tccggcgtca ccacctgaac cgtgaagtca
      841 ttgatcttgc tgacggcgaa tgctttgccg ccgatgtgaa acaggtcgaa cgtgccttgg
      901 ttgaactccc ggttatacat gatgtcggtc caggtaaaca ccacgtcatc ggccgtcagc
      961 ggttcgccgt cgctccagcg cacgccctgc cgcagcttga aggtccacgt cctctggtcc
     1021 ggggcgacgg accacgactc ggccaacccc gggccgggct gctgcgtggc ccaatccaga
     1081 ttgaccagcg aggcaaagag caggcggatg atgccgtcgg acgcgctgtc cagagcgaac
     1141 aaggggttga atgtctttgg gctggcagtg gcggcgataa ccaggcgccc gccgtgccgc
     1201 ccaggctcgc ctttggcaat gagcggggag gagggcagag gatgcgatgc gccgggcttg
     1261 tcagccgcgg gtttcccgca gccagtcagc accagcgcga ttccgagcaa tgcaatgcga
     1321 gaaaaggaag gcgcggtcat cgtgatgccc tccctatagc ggattcatcg gaagcaggca
     1381 aggcggcatc aagctcgcgg aaaccgctcg tgcatggcaa gtcgctatgg tgtctccacg
     1441 gtattgctgc ggtattgcta cggtatctcc ccggtgtatc tcccatgttg atgccatcaa
     1501 catgggagca acaccgggga gataccgggg agataccggg gagataccgt gcctacaccg
     1561 tgccggctgg cttgggggta tgctgggggc ggcccgggga ttctcaattc tgagaaaggc
     1621 actcgccacc gcccccctcg caggtaatcg gcctggcctc ctcattttta ccacggtcag
     1681 cttggcgcag caggcggggg ccggccagca acgcgtgttt atcgggggcg gggctcgaaa
     1741 aggtcgtatt cacgccccaa gcttgaaaag cccgcgtgga agcgtccaaa gaaagcgcta
     1801 acccctgagt ttacaacggg ttagtgagta tcatgaattg gccctgggca gcagtggact
     1861 aacccgggtt aaccgcgctg gcggggctcg ttaatttggg ttttgccttc gggcggtttt
     1921 ggggtcatgc tgggggcatg gcgtctgggc cgctagtagc tgtggtggtg gttttgatct
     1981 ttgcgggagc aagcttcttc ttcgcactcg cggagacggc gctcttttcc ctgagtaaat
     2041 ggcaggtgcg gcagttggcg gaacgggcgc caggcgcggg cggaatcgtg gcgcgcttgc
     2101 tggctgagcc gcaggatttg ctggcgacga tggtgctggg caatacgttc gccagcgcgg
     2161 ccatgctggc ggtggcgcta tggatggcca tccacgggcg ctggccgctg gcggcgacgg
     2221 tggtgggcct gctggcactc atcctgattg gctgcgaggt gctgcccaag actctggcgg
     2281 tgcggcgggc ggagcaatgg tcgttgcggg tggcgcggtc gctgtcgttc gtggtgcaat
     2341 tcagcctgcc gctgtgccgg gtggcgcaga aggtcaacac ggccattctt cgggtggtca
     2401 tgccggcaac ggggcagccg cacatcacgt tgacggatgc ggattaccag gagttgctgg
     2461 agatggcgta tcagcaaggc acgctagcgc aatcggaaaa ggagatcatc ctgcagatta
     2521 tcagcctcga ccggcgcacg gccaaggaag tgatgaagcc gcgttcgcgg atggcggcca
     2581 tctcggatga tctgtctatc gaggacatga tcgctgccgc gtgcaagcac aagcatcgcc
     2641 gcctgccgat ctacgacgag acgccggata ctatcgtcgg catcctcaac acgcgggcgc
     2701 tgctgctcga tccgcaaatt gacctggcgg atgcgatcga gtttccctcc ttcgtgccgg
     2761 agacgatgaa cctgctgcaa ctgctcaaga gcctgcaacg gcagcagcgc ggcctggcga
     2821 ttgtgctgga tgagtttggg ggcaccgcag gcattgtcac catggaggat attctggagg
     2881 agctgatcgg gaagattcac ggcgaggcgg aagtcgaagg gttcgtgatg gagaaactcg
     2941 gcccgggccg ctggcgggtg gcgggcacgt tgcggctgga cgatttccgc cgcgagtatc
     3001 ccgtcctggg cgacgtgccc gaggtcgaaa ccatgggcgg gctgctgacc agcctgctcg
     3061 acgtggtgcc ggccaagggt gaggcggcaa cgttctgcgg gctcaagctc acggcgcagg
     3121 tggccgacga gcgccgcgtg cgggagttgc tggtggaggc ggtgaagtga tatgagccga
     3181 gactccgcag tctgggtggt cttcgtcttg tgcctcgtgg tgtcgtttgt cttgtcaggc
     3241 atggaggcgg gggtgtttac gttgagccga ttgcgcatcc ggcagcaaat gcgcgcaggt
     3301 caggcgtcgg ccaaggtgct gcacgactac ctcgaacacc cggagaactt cctctggacc
     3361 atcctggtcg gcaacaccgt ggcgaacttc ctcatcctgg gctggctcgt ggcgcaactg
     3421 cacgaggcgc tgggcgctta ccgggtgtgg ttcgtggtgg tgttttcggt cgcggtcttc
     3481 ctcttctaca cgttctttga tttgctgccg aagatgctgt tccggatata tccgaaccgg
     3541 ttctgcgtct tgctggcgcg tccgtttcgg ttgatccatt gggtgttgcg tccgctggtg
     3601 gcgctggtgg aaaccttctc gggcgcgctg gtgcggtggc ggggcggcaa agccttcacc
     3661 ccgcaccttt tcggcaaccg cgaggaactg cggctggtca tgcaggagtc cgcgcagggc
     3721 ttcacctcgg aggagcggac gatgatcaac cgggtgcttg atttgcaaac gctgacggtg
     3781 cgccaggcca tgaagccgct ggagcaggcc gtcgccataa ctgcccagac accggttagc
     3841 gccgcgctgg cgctgtgccg ggaacggaag tttacgcgcc tcccggtatg ggagaaccgc
     3901 gacggccagc agcgtattgt tggcttgctc gccttgaaca cgctgctcta ccagccggcc
     3961 ctggatacga ccaaaccggt gggcgaatat gtgaaacccg cgctcttcct ggatgaagac
     4021 ctgcggctcg aagtcgccct gcggcgcttg cagcggagcg gacagcggct ggcgattgtg
     4081 ctgggtcgcg accggcgcga gataggcatc cttaccctgc aagacttgct caaagggatc
     4141 ttcggggagg tgagcttgta gttatggatt gggacgtcct tttctccatc atactgcgcg
     4201 tcctgggcgt ggtggtgctc gttctactca atgcgttctt tgtctcctgc gagttttcac
     4261 tcgtcaaggt gcgagacacg caattgagcc cactggtgca tcgaggtcat cggcgcgcac
     4321 gggtcgccga ctacatctta cgtcggctgg acgcgttttt gagcgcggcc caactaggca
     4381 taacactgac cagccttggc ctgggatgga tcggcgaacc ggtgttcgcc gttttgttgg
     4441 gaccggtgtt cggcctgctg aatgtggact ccccggaaat gcagaaattg ctggcgtttg
     4501 tcatcggctt ctccgtgatc accttcctgc acatcagcgc gggggaacag gcgcccaaat
     4561 ggctggccat ccagaagccg ctggcgacgg cgctgtggat ctcttatccc ttgctgtggt
     4621 tctaccgagc gtcctatccc tttgtggtgg tgctcaactg gtcgtcccag tggttgctgc
     4681 gccaggccgg catcaaactg ggagacgagg ctggccgcgc gcattccgag gaggagttgc
     4741 ggctgctgct ggcgacgtcc cagaagcact ccggcgccac cgtcctgggc cgggacatcg
     4801 tcttgaatgc gctggacctg cggcggcgcg tcgcgcgcga ggtcatgcgg ccgcggcagg
     4861 aaatcgtcgc gctggatacc gaggcgagca tcgccgaatg tctggatgtg gcggaaaaga
     4921 cgcgctactc ccgctttccg ttgtgcgagg gcggggacct tgaccggaca ctgggcgttg
     4981 tccacatcaa ggacctgtat gccatgcgaa tcagagcccg aagcggtgcg gacttgcttc
     5041 ccgcagtgcg caagctgatc tatgtacctg agagcgcgcg gctggagaag ctattgcagt
     5101 tcttcctcga acggaagctg cacctggcga ttgtcgttga tgagtttggc ggcactatgg
     5161 gcatggtgac gctcgagaat atcctggaag aactggtcgg ccagattcag gatgagtttg
     5221 accaggagaa gccgttgctg acgcgaacgg gtgagaacac ctgggaagta gcgggcacac
     5281 tggcgctgca tgaattggaa gaggtcgtgg gcgaaccgct gcaagcggag ggcatcacca
     5341 cggtcagcgg ctgggtgaca cagcggctcg gcggctttcc caagccgggc gatgtgctag
     5401 ccatcggacc ttgcgagctg cgcgtggagg aaatggcggg gatgcgcgtc gcacgactca
     5461 agctgaccaa gaaacagcct gaagtctgaa ccgcgcctca ccaccgcgcg ccgcggatgg
     5521 cgaagacggt cacgaccagg ctgcccaggg agatcagccc gacggccggc cagaggtgat
     5581 gcacggcggc gcctgtcgca aagaggaagt tcagcaagag ccaaaccacc agcgaggaat
     5641 agtccggtgg tggctgtggc atcggtttct tgttaatgag cgagggcgtg gcgaacgcga
     5701 gaatgagcag cgccgtgacc gcccagccca ggaagttgac gcacggcgtg ctgtaccaat
     5761 cgaacttgag cttggtcgga ttccagaacc agtaatgctt tacggaggtg gcgaacggtt
     5821 ccaggccaac gtccagcagc acgacgagca ggacggtcag tccgatcagc ctgaagccgt
     5881 agttctgggt cttccgccag ggacgcaacg tcagccgcgc gacaccccgg gaagcaagta
     5941 tgaccgcaat ccacatcagc ggcaccgccc agggcagggg ataaaagagc tgctggccaa
     6001 tgcgctcggt atagacgtaa ggcccgaagg ggatagcagt caacgcgccg agggtctgta
     6061 ccgcgccggc gataaaggcg atgataatgg aggccagcat cacattctgg ccgggcaact
     6121 ggcgggtgag cgaggcgagc actatcgccg tcgtcaggac caccaggagc ccatccggcc
     6181 agcg
//
LOCUS       DATCGS010000554         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_197382, whole genome shotgun sequence.
ACCESSION   DATCGS010000554 DATCGS010000000
VERSION     DATCGS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197382"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..>2246)
                     /locus_tag="VIL39_11415"
     CDS             complement(170..>2246)
                     /locus_tag="VIL39_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5482082.1"
                     /translation="VKLIIDGRKAEVTAGQTILQAARTLGLSIPTLCHVEGFEPSASC
                     FVCAVKIEGRPNLWPSCATPAAEGMVVITNSDEVRAARKTSLELLLSDHAGDCIGPCR
                     TGCPARLDIPGFIAGIAAGDHGKAAQIVTDDLTLPASLGRVCPRLCEERCRQCDVEEA
                     LSIRNLHRFTADYRRRTEMPAEPAGGTPAPLPAPVGRRVAIVGAGPAGLAAAHHLLRR
                     GHGAVVFDAHPQPGGMLRYGIPEYRLPRQVLDAEIQTIRTLGAEFRLGKRLGRDFTLD
                     DLRRDFDAVFLAIGAQGSRGLGCPGEELATPALEFLERLTDGPPPTVGSDVIIVGGGN
                     TAMDASRCAIRLGAKSVRVIYRRSRREMPCLMEEVEAAEAEGVIIEFLVAPLRLERKA
                     GTLILTCQRMELGALDASGRARSLAIADSHFDVQASCVIAAIGQAVDLEPLNSIQLTL
                     SKWGIAADPKTLATNLPGVFAGGDAVTGPDLAVRAVAAGKLAAASIDQHLSGKKVIGA
                     PESVHVLLGKLSEDELAIFLRGIEQAPRAPMPHLPMDKRRTTFEEVELGFSLETATRE
                     SRRCLGCGCGKAIPCRLRQFATDYGVDPQRFVGQRRHFARDSSHPEIIFEPGKCIICG
                     ACVKVAAQAGEELGLSFIGRGFQVSVAAPFHKPMAEALRKSARRAAEVCPTGAIMLKG
                     TGCADCRLA"
BASE COUNT          402 a          770 c          713 g          361 t
ORIGIN      
        1 gggcggttgc cctgaaatcc gaaaaccgag accctaaacc caaaggaagt ccgaaatcca
       61 ataagggcct acactgtttg aggcgccgaa ttggaacctc aaatccgaga cctctcgctc
      121 ctcacgggca cagcaaatcc agccgggaac cgcctggcgt tgcgctcaat cacgccaatc
      181 ggcagtcagc gcagccggtg cctttgagca tgatcgcgcc agtggggcag acctcggcgg
      241 cgcgccgggc ggacttccgc agggcctcag ccatcggctt gtggaaaggc gcggcgactg
      301 acacctggaa cccgcgcccg atgaaggata gccccagttc ctcacccgcc tgggccgcca
      361 ccttcacgca ggcgccgcag atgatgcatt tgcccggctc gaagataatc tccgggtggg
      421 agctgtctcg cgcgaagtga cgacgctggc cgacaaagcg ttgcgggtcc acgccgtaat
      481 cggtcgcaaa ctggcgcagc cggcagggga tcgccttgcc gcagccgcaa cccagacagc
      541 gccgcgattc gcgggtcgca gtctccagcg aaaagccaag ctccacttcc tcaaaggtgg
      601 tccgccgctt gtccatcggc aggtgcggca tgggggcgcg cggtgcctgc tcaatccccc
      661 gcaggaatat cgccagctcg tcctcgctca gcttgcccag gagaacgtgc accgactcgg
      721 gtgcgccaat gactttcttc ccgctgaggt gttgatcaat ggatgccgcc gcgagcttgc
      781 cggcagccac cgcccgcacg gcgaggtctg gcccggtgac cgcgtcgccg ccggcgaaaa
      841 cgccaggcag atttgtagcg agggtcttcg ggtcggcggc gatgccccac tttgagagag
      901 tgagctgtat gctgttgagt ggctccaagt ccacggcctg tccaatggcc gcaatgacgc
      961 aactggcctg cacgtcgaag tgcgaatcgg cgatcgccaa cgaccgggcc cgcccacttg
     1021 cgtccagcgc tcccaattcc atccgctggc aggtgaggat caacgttccc gccttgcgct
     1081 ccaggcgcag cggagcgacg aggaactcga tgatcacccc ctcggcttcg gccgcttcaa
     1141 cttcctccat gaggcaaggc atctcgcggc gactacggcg gtagatcaca cgcacggact
     1201 tcgcgccgag tcggattgcg cagcggctgg cgtccatggc cgtgttgccg ccgcccacga
     1261 tgatgacgtc actccccacg gtcggcggtg gaccatcggt cagcctttcc agaaactcca
     1321 atgcgggtgt ggccagttct tccccggggc agcccaagcc gcgcgagcct tgagcgccaa
     1381 ttgccaggaa caccgcgtca aagtcccgcc gcaggtcgtc gagcgtgaag tcccggccca
     1441 ggcgtttgcc caggcggaac tcggcgccca gcgtgcggat tgtctggatt tccgcatcga
     1501 gcacctgtcg cggcaggcga tactccggga tgccgtaccg cagcataccg cccggttgcg
     1561 ggtgcgcgtc aaacaccacc gcgccatgac cgcgccggag cagatgatgc gccgccgcca
     1621 gccctgccgg gccagccccg acaatagcca cccgccgccc caccggcgca ggtagtggcg
     1681 caggcgtccc gcctgcgggt tcggcgggca tctccgtccg ccgccgatag tccgccgtga
     1741 accgatgcag attacgtatt gaaagcgcct cttccacgtc acactgccgg cagcgctcct
     1801 cgcataaccg cgggcaaacc cgtcccaggg acgcaggcag cgtgagatca tcggtgacaa
     1861 tctgcgcggc tttcccgtgg tcgcccgcgg caattcccgc gatgaagccg gggatgtcca
     1921 gcctggcggg gcatccggtc cgacaagggc caatgcagtc gcccgcgtgg tcggagagca
     1981 gcagttccag cgaggtcttg cgcgccgccc gcacttcgtc ggaattggta ataacgacca
     2041 ttccctccgc tgccggtgtg gcgcaggagg gccagagatt gggccgccct tcgatcttca
     2101 ctgcgcacac gaaacaggag gccgacggct cgaagccctc gacgtggcag agcgtcggga
     2161 tggagagacc gagggtgcgc gccgcttgca ggatggtctg gccggctgtg acctcggctt
     2221 tgcgaccgtc gatgatgagc ttcacg
//
LOCUS       DATCGS010000555         6370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_294070, whole genome shotgun sequence.
ACCESSION   DATCGS010000555 DATCGS010000000
VERSION     DATCGS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294070"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VIL39_11420"
     CDS             <1..1900
                     /locus_tag="VIL39_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEY5482083.1"
                     /translation="EFGIEHPVGMCLQDAGWRFGPWLQHEPGAYTPSEYVTWNQYFES
                     VAQPRSAPVWRVSQEDVQVSLVWGAQVLQRIAQQVRGAENRLAMAEKLASLAFVYGQT
                     PWPEASLDEAWRLLLLAQHHDCWIVPYNGRPGETWADKVRLWTDGTHNTSEEVIGQST
                     SALMPHLDGGAGYAVRVFNTLGVARTNLVSLALPDAWDGPGASVWDSAERQLASQTVG
                     DKGRRELLFVAPVPALGYQTFWLKPASDAQVRGATLVQLADGSVRLETDYYRAELDPN
                     RGGTIRSLVAKRLGNRELVDSANSRRFNEIRGFFFKEQCFRSSADAAAKVEIVEAGPV
                     RVRLRVSSQIASNAVTQWITLVQGEPRIDLSVTIDWQGNPGIGADYYQAGGFRREQDR
                     KAFYDERYKLLALFPLNLHRQEVFKDAPFDVTESRLTNTFFETWSEIKNDVILHWVDV
                     RDAAQEFGVALLSDHTTSYAHGADHPLGLTLQYRGVGLWGRDYSVRGPTEVKYALLPH
                     ATDRQSAAVWTAGTAWNEPLVARLSPSGVETGAGEKSLLTIDGADWEVPAMRVSAGKI
                     LVRLFNPSSDARGRIVRYGAPASKVEMVQLNGQFIKELTVRKGARGGTVFELALPSKG
                     IGTLRITP"
     gene            2176..3618
                     /locus_tag="VIL39_11425"
     CDS             2176..3618
                     /locus_tag="VIL39_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY5482084.1"
                     /translation="MNTEQLYQERLNRYITAMRNEKPDMVPIRPFVAEFTAKYAGYTC
                     QEVAHDYNKAFEAAIKTAKDFGWEAVVPNMVYVWTGLAQAVGLRYYGIPGIGIPHESG
                     FNYIEPAEDQAFMRADEYDALIDDPTAFLYNVWLPRASTEVSKLGSPSTYRNNLSFVK
                     GAMAMLSYFYAFGPQIARLRTECGTASAIAGIFKAPFDILADKMRGYVGLTMDMATQP
                     DKVLKACEALMPHLCAVGLGTADPGKQVPIGFWMHRGCVPFINPKTFASHNWPTLKPI
                     IEEFWKHGNQTLFYGEGKWHHHFDAFRELPDRSIVFHCDQDDIFHAHRKLHDKFALSG
                     GIPNAMLSYGKPDEVRAFCRRVIDEVARDGGYIMDAGAIMQDDTSIENIRAMTETARQ
                     YGVYSAGSFKAPVATPPCEVPASVADRKKLNGMKGRPEPRVRPGVCFPWEERVKDLPE
                     ITGSPELVRKIWEDIDAFGNMYIWQLLLSF"
     gene            3693..4838
                     /locus_tag="VIL39_11430"
     CDS             3693..4838
                     /locus_tag="VIL39_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5482085.1"
                     /translation="MPTTRPASSARFELKADGPGVGALTLSGRLDARSTADLWRDLDA
                     RLRHSPVSALEVDAAGVEYCDGAGLALLHFLSLGGMLPPGGKATVRGLRPEFQNLFRR
                     FTTEDYQRNRPQAPKPNSVPEEVGRAVVAAANEIEEEVAFIGEVSRGLVANLARPKAM
                     RWGEVWRVFEQAGVNALPIISLISLLIGLIIAFEAASPFAMFGAQIFIANLLGITMAR
                     ELSGLMTAIILAGRSGSAFAAELGTMKVTEELNALETFGLNPIRFLVVQRVLAGILLT
                     PLLTIYSIFVAILGGVLVMVAMGFPLVTVYHQMAQTLGYKDILIGVGKGVIFGALIAG
                     VGCLRGLQTRQGPSAVGDSTTRSVVTGILLVIVADAVVAVLLYLLHL"
     gene            4835..5620
                     /locus_tag="VIL39_11435"
     CDS             4835..5620
                     /locus_tag="VIL39_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5482086.1"
                     /translation="MNESKEAVITVKALTVGYGEKVLLEKLDFEVRRGEVFVILGGSG
                     CGKSTLLKHMIGLYPPMAGEVWFGPDNLVAAQGDDRIRILRKFGVMYQSGALFGSLTV
                     LENVSLPLDEFTDLPTQAKELIALAKLKLVAMETAASQMPADLSGGMQKRAAIARAMA
                     LDPGILFLDEPSAGLDPITSAGLDALIKDLSRTLGCTFVIVTHELPSIFAVADRVIML
                     DGKTKGIIGTGRPEELRDHSPNPWVRQFFNRQAEPSPSPPTTP"
     gene            5637..>6370
                     /locus_tag="VIL39_11440"
     CDS             5637..>6370
                     /locus_tag="VIL39_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5482087.1"
                     /translation="MSAKTNHFKLGLFVLGAVALLFAGLLAFGAKSYFSPKTRFETAF
                     PGEVSGLSVGSRVEFRGVPVGKVTRITFAWVLYPQSKSRLIIVQFDVDDEMVLLPPDM
                     SMKLALQQMTDKGLRAMVKGQGITGTSLLAMEMLNPNSYPPLPLDYKPRALYVPSAPA
                     QFTRMLEAIERSLDHIQRLDIVGIGQGITNALGSVAQLSDKLGKLDLQAISTNANSVL
                     VEVRNAAVKLQDAVGQVQETIKAMKL"
BASE COUNT         1210 a         1952 c         1977 g         1231 t
ORIGIN      
        1 tgagttcggc atcgagcatc cggtaggtat gtgcctgcag gacgccggtt ggcgcttcgg
       61 gccgtggctt cagcacgagc cgggcgcgta caccccctcc gaatacgtga cttggaacca
      121 gtacttcgag agcgtggcgc agccgagatc cgcaccggtt tggcgtgtca gtcaggagga
      181 tgttcaagtc agcttggtct ggggagcaca ggtgcttcag cgtatcgccc agcaagtccg
      241 tggcgcggag aataggctcg cgatggccga aaaactggcc tcgcttgcct ttgtttacgg
      301 ccaaacaccc tggccggaag cctcattgga cgaggcatgg cgactactcc tcctggcgca
      361 gcaccatgac tgctggattg ttccctacaa tggccgtcct ggtgaaactt gggccgacaa
      421 ggtgcggctt tggaccgatg ggacccataa caccagcgag gaagtgattg ggcaatctac
      481 ctctgcgctg atgccgcatt tggatggggg tgccggctat gcagttcgag tcttcaacac
      541 gcttggtgtg gcgcgaacca acctggtgag cctggcattg cctgatgctt gggatggccc
      601 cggggccagc gtttgggaca gtgcggagcg gcaactcgca tcacaaaccg ttggcgacaa
      661 aggtcggcgg gagctcctct ttgtcgcgcc ggtgccggcg ttgggctacc aaaccttttg
      721 gctcaagcca gcttccgacg cccaagtcag aggcgcaacg ctggtccagt tggcagatgg
      781 cagcgttcgt ttggagaccg actattaccg ggccgaactg gacccgaaca ggggcggcac
      841 cattcgaagt ttggtcgcaa agcgcctcgg caatcgggag ttagtcgatt cggccaattc
      901 gcgacgcttc aatgaaatcc gcggcttctt ctttaaggag caatgctttc ggtcgagtgc
      961 cgatgcggcg gcgaaagtgg agatcgtgga agcggggccg gtccgggttc gtctgcgcgt
     1021 cagcagccaa atcgcctcta atgcggtgac gcaatggatc accctggtcc agggagaacc
     1081 gcgcattgat ctgagcgtga ccattgattg gcagggtaat ccgggcatcg gggcggatta
     1141 ttaccaagcg ggcggtttcc gccgggaaca ggaccgcaaa gccttttacg atgagcgcta
     1201 taagctgctg gcgctgttcc cgcttaactt acaccgccag gaggttttca aggatgctcc
     1261 atttgacgtc accgaaagcc ggctgacgaa caccttcttc gagacgtggt ctgagattaa
     1321 gaacgacgtg atacttcatt gggtggacgt ccgtgatgct gcacaggaat ttggcgtcgc
     1381 gttgttgagc gaccatacca ccagctacgc gcacggtgcc gatcatccgc tcggacttac
     1441 actgcaatac cgtggggtcg gcctttgggg ccgagactat tctgtccggg ggccgaccga
     1501 agtgaaatac gcgttgttgc cccacgcaac cgaccgccag tctgccgcgg tgtggaccgc
     1561 cgggactgcc tggaatgagc ctttggtcgc ccggctgagt ccttccggcg tggagaccgg
     1621 cgcgggcgaa aaatcgctgc tgacgattga cggcgcggac tgggaggtgc cggccatgag
     1681 ggtcagcgcg ggcaagattc tagtccgctt attcaaccca tcgtcagatg cacgcgggag
     1741 aatagttcgg tatggcgctc cggcgtcaaa ggtcgagatg gttcaattaa acggccagtt
     1801 catcaaggaa ctcacggtgc gaaaaggcgc aagaggcgga acagtcttcg agttggcgtt
     1861 gccctccaaa ggaattggca cgctcaggat tactccctaa ccgggattga tgcgccctca
     1921 ctgtcgcttg ctgcgatgcc ttcgccgatt aaacgagcgg cacacgcagt ttatcgggca
     1981 aatcggcgcc gtctggtggc tgcggcgtcg ccagccggag gcggagcgtc ccttcaaaat
     2041 gtggctttac ccgcttccgg cccttgtcgc cctggtgggg tggttgtttt tgttcgggac
     2101 gaccgatctc accactgtca gcgcgatttt gctagtctgc cactccgcca gacggctatg
     2161 ctccttcgca gccttatgaa cacggaacaa ctctaccagg aacgcctcaa tcgttacatc
     2221 accgccatgc gcaacgagaa gccggacatg gttcccatcc ggccgttcgt cgcggagttc
     2281 actgccaaat acgccggcta cacctgccag gaagtcgcgc acgactacaa caaggccttc
     2341 gaggcggcca tcaagaccgc caaggatttc ggctgggaag ccgtcgtgcc gaacatggtt
     2401 tatgtctgga cggggctcgc gcaggcggtc gggctccgct attacggcat tcccggcatt
     2461 ggcatccccc acgaaagcgg cttcaactat atcgaaccgg ctgaagacca ggcgttcatg
     2521 cgggccgacg aatacgacgc gctcatagac gaccccacgg cgttcctcta caatgtttgg
     2581 ctcccccgcg cctcgactga ggtcagcaaa ctcggcagcc catccactta ccggaacaat
     2641 ctctccttcg tgaagggcgc catggcgatg ctttcctatt tctacgcctt cggtccgcag
     2701 atcgcccggc tccgcaccga atgcggcacg gcctcagcca tcgcgggaat cttcaaggcg
     2761 cccttcgaca tcctcgccga caaaatgcgc ggctatgtcg gcctgacgat ggacatggcg
     2821 acccaaccgg acaaggtcct caaagcgtgc gaagcgctca tgccccatct gtgcgcggtg
     2881 ggcctcggca ccgccgaccc gggcaagcaa gtccccatcg gcttctggat gcaccgcggc
     2941 tgcgtgccat tcatcaaccc gaagacgttc gcctcccaca attggccgac cctcaagccc
     3001 atcattgagg agttctggaa gcacggcaac cagaccttgt tctatggcga aggcaaatgg
     3061 caccaccact tcgacgcctt ccgcgaattg cccgaccgca gcatcgtctt ccattgcgac
     3121 caggacgaca tcttccacgc gcatcgcaaa ctgcacgaca agttcgcact cagcggcggc
     3181 atccccaatg cgatgctcag ctacggcaaa cccgacgaag tccgcgcctt ctgccgccgg
     3241 gtcattgacg aggtcgcccg cgacggcggg tacatcatgg atgccggcgc catcatgcag
     3301 gacgacacca gcattgagaa tatccgagct atgacggaga cggcgcgcca atacggcgtt
     3361 tacagcgcgg gcagctttaa ggcgcccgtc gccacaccac cgtgtgaggt gccggcctcg
     3421 gtggcggacc gtaagaagct caacgggatg aaaggccggc cggagccgcg cgtccgcccc
     3481 ggcgtctgtt tcccctggga agagcgcgtc aaggaccttc ccgaaatcac cggcagcccg
     3541 gagctcgtgc gcaagatctg ggaggacatt gacgccttcg gcaacatgta catctggcag
     3601 ctcctcctga gcttctagcg catgaaagcg agaatcgatc acacagcaac gagaaccaac
     3661 cagagtccaa actgcttcgg agaggcctct ccatgccaac cacacgcccc gcttccagcg
     3721 cccggttcga attgaaggca gacggccccg gcgtcggcgc gctgacgttg agcgggcgtc
     3781 tggatgcgcg ctccacggct gacttgtggc gagacctgga cgcgcggctg cggcacagtc
     3841 cagtctcggc gctggaggtg gacgcggctg gggtggagta ttgcgacggc gccggactgg
     3901 cgctgctgca cttcctgagc ctgggcggca tgttgccgcc gggtggcaag gcgacggtcc
     3961 ggggactccg gcctgaattt cagaacctct tccggaggtt cacgaccgag gattaccagc
     4021 ggaatcggcc gcaggcgccg aagccaaact ccgtccccga ggaagttggc cgggcagtcg
     4081 tggccgcggc gaacgagatc gaagaagagg tggcgttcat tggcgaggtg tcccgcgggc
     4141 tggtggcaaa cctcgcgcgg cccaaggcca tgcgctgggg agaagtgtgg cgcgtctttg
     4201 agcaggcggg ggtcaacgcg ctgccgatta tctcgctgat cagcctgctg ataggattga
     4261 tcatcgcgtt cgaagcggcg tcaccgttcg ccatgtttgg ggcgcagatc ttcatcgcca
     4321 acctgctcgg cattaccatg gctcgcgaac tcagcgggct gatgaccgcc atcattttgg
     4381 cgggccgctc cggttcggcc ttcgcggcgg aactggggac gatgaaggtc accgaggagc
     4441 ttaacgcgct ggaaaccttc gggttgaacc cgatccgctt tctcgtcgtg cagcgcgtgc
     4501 tggccggcat cctgctcacc ccgctgctga cgatctactc catcttcgtc gccatcctgg
     4561 gcggcgtatt agtcatggtg gccatgggtt ttccgctcgt gacggtttac catcagatgg
     4621 cccagacgct gggctataag gatattctta tcggtgtggg caaaggggtc attttcggcg
     4681 cgctcattgc tggcgtcggc tgcctgcgcg gcctgcaaac caggcagggt cccagcgccg
     4741 tgggagattc caccacgcgc tccgtggtga ccggcatcct cctcgtgatc gtggcggatg
     4801 cggtcgtcgc cgtgctgctc tacttgctgc acctatgaac gaatccaagg aagccgtcat
     4861 cacggtgaaa gcgctcacgg tgggctacgg ggagaaggtg ctcctggaga agctcgactt
     4921 cgaggtccgg cgaggcgagg tgtttgtcat cctgggtggg tcgggctgcg gcaagagtac
     4981 cttgctcaag cacatgattg gtctgtatcc gccaatggcc ggcgaggttt ggttcggccc
     5041 ggacaatctg gttgccgccc agggcgacga ccgaatccgc atcttgcgca agttcggtgt
     5101 gatgtaccaa agcggcgcgc tgttcggctc gctgacggtc ctggagaacg tctcactgcc
     5161 gctggatgag ttcacggatt tgcccaccca agccaaggag ctgattgccc tggccaagct
     5221 gaagctggtg gcgatggaga cggccgccag ccaaatgccc gcggatttga gtggtggcat
     5281 gcaaaagcgc gccgccatcg cgcgcgccat ggcgctcgac ccggggatac tctttctgga
     5341 cgagccgtcg gccgggctgg accccatcac ctcggccggg ctggacgctt tgattaaaga
     5401 cctctcccgc accctcggct gcaccttcgt catcgtaacg catgagctgc cgagcatctt
     5461 cgccgtggcc gaccgcgtca tcatgctgga cggcaagacc aaggggatca tcgggaccgg
     5521 ccgcccggaa gagttgcggg atcactcacc taatccctgg gtgcgccagt tctttaaccg
     5581 gcaggccgag ccgtcccctt cgccacccac caccccatga atgaccggag ttctctatga
     5641 gcgcaaaaac caaccacttc aaactcgggc tcttcgtgct gggggccgtc gccctcctgt
     5701 tcgccggctt gctggccttt ggcgcgaaga gttacttctc accgaagacc aggttcgaga
     5761 ccgccttccc tggcgaagtg tccgggcttt cggtcggctc gcgcgtcgaa ttccgcgggg
     5821 tgccggtcgg caaggttacc cggatcacgt tcgcgtgggt gctgtatccg caatccaaat
     5881 cgcgtttgat tatcgtgcag ttcgacgttg acgatgagat ggtccttctg ccgccggaca
     5941 tgagcatgaa gttggctctg caacagatga cggacaaggg attgcgggcg atggtcaaag
     6001 gccaggggat cacgggcacc agcctcctgg cgatggagat gctcaacccg aacagctatc
     6061 cgcccctgcc cctggattat aaaccgcgcg cgctctacgt cccctccgcc ccggcccagt
     6121 tcacgcgcat gttggaggcc attgaacgct cgctggacca cattcagcgg ctggacattg
     6181 tcggcattgg ccagggcatc accaacgcgc tgggatcggt cgcccagctc tcggataagt
     6241 tgggcaaact cgatctccag gccatcagca ccaatgcgaa ctccgtgctg gtggaagtca
     6301 ggaatgccgc agtcaaactc caggacgcgg tcggccaggt gcaggagacc atcaaggcca
     6361 tgaagctgga
//
LOCUS       DATCGS010000556         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_147012, whole genome shotgun sequence.
ACCESSION   DATCGS010000556 DATCGS010000000
VERSION     DATCGS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147012"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VIL39_11445"
     CDS             <1..555
                     /locus_tag="VIL39_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482088.1"
                     /translation="RETNWVGFTAGFQVLARTQSGELWEPLSSPPSAEDPATSTCRLV
                     ASNALPDHLAMAYCEKGLLYEVRPDGTLWKTDYSWSAQAPTSSAGKWHHVGKRSDWLS
                     IWGVGGTAFGLTSDGTLWTWGIDPSRPPVLDLSAKLDLVQIRLRTYFAGRPTRIQAPR
                     MSAWHGEPRPLMRLVPVTPSNSRI"
     gene            complement(643..924)
                     /gene="rplW"
                     /locus_tag="VIL39_11450"
     CDS             complement(643..924)
                     /gene="rplW"
                     /locus_tag="VIL39_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY5482089.1"
                     /translation="MNSFEIIRTVRLTEKGTRQGEKYNQYTIMADPRASKPQIRTAVQ
                     DLFKVKVVKVNTLNVRGKFRRQRTKQAGQSPDWKKAIVTLKAGDKITLT"
     gene            complement(926..1552)
                     /gene="rplD"
                     /locus_tag="VIL39_11455"
     CDS             complement(926..1552)
                     /gene="rplD"
                     /locus_tag="VIL39_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY5482090.1"
                     /translation="MKLTIKDIKGKDQGELEVKFPLIEDGRGTQAVHDTVVAYQAAQR
                     MGTACTKTAGDVAGTNKKPWRQKGTGRARAGSFQSPLWRGGGVVFGPKPRDFTKKISH
                     NTKQLALRKAMSERLRAGDVVVVDDLKLGSGKTKELAGALSALELKGTALIVAHTADA
                     NLMRASRNLADVALTTSAVLCTYDVLRPDKLVFTKNAFEQIEARLGKE"
     gene            complement(1562..2260)
                     /gene="rplC"
                     /locus_tag="VIL39_11460"
     CDS             complement(1562..2260)
                     /gene="rplC"
                     /locus_tag="VIL39_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5482091.1"
                     /translation="MPLGLLGKKLGQTRVYDAKGVITPVTIVLAGPNRVLQCKTQQSD
                     GYNAVQLGFGDQKEQRLTKPLLGHIKKFNGQPVKRILEFRDFTKEVKPGDVVGPTLFA
                     PGDYVDAIGLTKGRGFEGVVARHAFRGGDSTHGSKGWHRRSGSIGQRLFPGTVMRGMK
                     MPGHLGQVRRTTQNLLVVQVREAENIILIRGAIPGSNGDYIVIRESKKRPKGWLPHAQ
                     RPENIKKSEGKKKK"
     gene            complement(2275..2583)
                     /gene="rpsJ"
                     /locus_tag="VIL39_11465"
     CDS             complement(2275..2583)
                     /gene="rpsJ"
                     /locus_tag="VIL39_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY5482092.1"
                     /translation="MAAQRIRIRLKAYDHRVIDQSARDIADTVKRTGARVAGPIPLPT
                     RIERFTVQRSPHCDKKSQETFEQRTHKRLIDILDPTAKTVDELKKLNLPAGVDITIKI
                     "
BASE COUNT          467 a          887 c          871 g          644 t
ORIGIN      
        1 cgtgagacaa actgggttgg attcaccgca ggctttcagg ttctggcacg cacgcagtcc
       61 ggtgaactct gggagccgct cagctcgccg cccagtgcgg aggatccggc aacctcaacg
      121 tgccgattgg tggcctccaa cgcgttaccg gaccatttgg cgatggccta ttgcgaaaag
      181 ggcctgctct acgaggtgcg gcccgatggc acgctttgga agaccgatta ctcctggtct
      241 gctcaggctc caacgtcctc ggctggcaag tggcatcacg tgggcaaacg atctgactgg
      301 ctctcaatct ggggagttgg ggggacggct ttcggcttaa cctcggacgg caccctctgg
      361 acttggggaa tcgatcccag ccgcccgccc gtgctggatc tctctgcgaa gcttgacctg
      421 gtccagatcc ggctaaggac ctatttcgcg ggaaggccca cccggatcca agcgccgcgc
      481 atgtcagcct ggcacggcga gcctcggcca ctgatgcgcc tggttccggt cacgcccagc
      541 aactctcgga tataacagcg gtctgctgca gccgttggac tgcgagcccc acctccccga
      601 accggctcct ccgcgagttc ggatacgaat agggggccag gactaagtca gggtgatctt
      661 gtcgcccgct ttcaaggtca cgatggcctt cttccaatct ggcgactgac cggcctgctt
      721 ggtgcgttga cggcggaact tgccgcgcac attcagcgtg ttgaccttga cgaccttgac
      781 cttgaacagg tcctgcactg ccgtgcggat ctgcggcttg ctcgcccgcg ggtcggccat
      841 gatggtgtac tggttgtact tctcgccctg ccgcgtcccc ttttcggtca aacgcacggt
      901 ccggatgatt tcaaaagagt tcatgctatt ccttgcctaa gcgcgcttcg atttgctcga
      961 aggcattctt ggtgaacacc agcttgtcgg ggcgcagcac gtcgtaggtg cacagcaccg
     1021 cgctcgtcgt cagcgccacg tccgccaggt tgcgcgaagc ccgcatcaga ttggcgtccg
     1081 ctgtgtgcgc cacaatcagc gcggtgcctt tcagctccag cgcgctcaat gccccggcta
     1141 actccttggt cttgccggag ccgagtttca ggtcatcaac caccaccacg tcgcccgcgc
     1201 gcagccgctc ggacatcgcc ttgcgcaacg caagctgttt ggtgttgtgc gagattttct
     1261 tggtgaaatc ccgcggtttg ggtccgaaga ccacaccgcc gccccgccac agtggcgact
     1321 ggaaagaacc ggcgcgggcg cggcccgtgc ccttctgccg ccacggcttc ttgttggtgc
     1381 cggcgacgtc gccggcggtt ttggtgcaag cggtgcccat gcgctgggcg gcctggtagg
     1441 cgactaccgt gtcgtgaacc gcctgggtgc cccggccatc ctcgatgagc gggaacttca
     1501 cttccagttc gccctggtct tttcctttaa tatctttaat cgtaagcttc atggcagcac
     1561 tctacttctt cttcttgcct tcgctcttct tgatgttctc tggccgttgc gcatgcggca
     1621 accagccctt ggggcgtttc ttggattcac gaatgactat ataatcgccg ttcgaacccg
     1681 gaatggcgcc cctgatcaga ataatgttct ccgcttcccg gacttgaacc accagcaggt
     1741 tctgggtggt gcgccgcact tggcccaggt ggccgggcat cttcatcccg cgcatgaccg
     1801 tgcctgggaa caggcgctgg ccgatggatc ccgagcggcg atgccagccc ttcgagccgt
     1861 gcgtcgagtc gccgccgcgg aaggcgtgcc gggccacgac gccctcgaac ccgcggccct
     1921 tggtgaggcc gatggcgtcc acgtaatcgc ccggagcaaa gagggtcggg ccgaccacgt
     1981 cgccgggttt cacttccttc gtgaagtcgc gaaactcgag gatgcgctta acgggctggc
     2041 cgttgaactt cttgatatgg cccagcaaag gcttggtgag ccgttgttcc ttctggtcgc
     2101 caaaaccaag ctgcaccgcg ttatagccgt cgctttgctg cgttttgcat tgcagcacgc
     2161 ggttggggcc ggcgagcacg atggtcaccg gggtgatcac ccctttcgcg tcatagacgc
     2221 gcgtctgacc cagtttcttt cctagtaatc cgagtggcat ggagtttaag ggggttaaat
     2281 tttaatggtg atgtccacgc cagcaggcag gttgagcttc ttcagctcgt ccacggtctt
     2341 ggcggtcgga tcgaggatgt ctatcagccg cttgtgggtg cgctgctcga aggtttcctg
     2401 ggacttcttg tcacagtgcg gcgaccgttg gacggtaaac cgctcgatgc gggttggcag
     2461 tgggatggga ccggcgacgc gggctcctgt gcgtttgacg gtgtccgcga tgtcgcgggc
     2521 ggattggtcg atgacccggt ggtcatacgc cttcagtcgg atgcgaatac gttgtgcagc
     2581 catacaaaga acaattgcct attaatggtc ttgttaaaat ttttccttcc cgatcttcgg
     2641 gaaacggaat gcggaatata tcaggagtaa ttccccgcgc aagaggtttt tccaaaaatc
     2701 ttgctggccg cttttggccg gtcctggccg gctcagcgcc ccgcccggcg tgcgggcgtt
     2761 ttactggctg gcggcgtccc ctttgcggcc cagggttgaa agcagcccgg ctgtagcctc
     2821 gaccatgccc ggctcgtatt gggccggctc gcgcacctca aaccgcctc
//
LOCUS       DATCGS010000557         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_740551, whole genome shotgun sequence.
ACCESSION   DATCGS010000557 DATCGS010000000
VERSION     DATCGS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740551"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VIL39_11470"
     CDS             <1..1277
                     /locus_tag="VIL39_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5482093.1"
                     /translation="TCGSVDDGKSTLIGRLLYDSRSLMEDQIEALERSADLTGGGRID
                     LSTLTDGLRAEREQGITIDVAYRYFATPRRRFIIADTPGHVQYTRNMVTGASSANLAI
                     VLVDARQGVTEQSRRHTCIAAMLRIPHLVIAVNKMDLVCWSEERFIEIRDQFEEFLPR
                     LEVKDVQFIPLSALTGDNVVEPSKHTPWYPGPTLLGHLEAVHIGSDWNLNGFRLPVQW
                     VNRPNRPTDPQLHDFRGFSGQIAGGIVRVGQKVMVLPGGLVSTVKQIWTYDGPLQEAF
                     CPQSITLVLDHDIDVSRGDMIIGPDNLPGASTNLRAQVCWMHPRALQPGRKYSLQHTT
                     RTVQAVITGIEDRIDMTTLEPEPNPPELALNDIGEVRLNTSAPLVFDGYAANRLTGSF
                     ILVEPGTNATVGAGLLLPPTEVAKPEYSDFAI"
     gene            1800..>2133
                     /locus_tag="VIL39_11475"
     CDS             1800..>2133
                     /locus_tag="VIL39_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014016814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5482094.1"
                     /translation="MVYLVGAGPGDAGLFTLRGKEVMERAEVVIYDGLVSRELLRFAP
                     PSAEIIYGGKHDRTRCVSQAELNALLLAKALAGKRVVRLKGGDPFVFGRGGEEAEALS
                     AAGIPFEVV"
BASE COUNT          408 a          726 c          635 g          364 t
ORIGIN      
        1 acacctgcgg ttcggtcgac gacggcaagt ccactctcat tggccggctg ctctacgatt
       61 cgcggtcgct gatggaagac cagatcgaag cgctcgaacg ctccgccgat ctcacgggcg
      121 gcggccggat agatctctcg accctcaccg acggcctccg cgccgaacgc gagcagggca
      181 tcaccattga tgtcgcctat cgttacttcg ccaccccgcg ccggcggttc atcatcgccg
      241 acacaccagg ccatgtgcag tacacgcgca acatggtcac cggcgcctcc tccgccaacc
      301 tcgccatcgt gctcgtggac gcgcgccagg gcgtcaccga gcaaagccgg cgccacacct
      361 gcatcgcggc aatgctgcgc atcccgcacc tggtcatcgc cgtgaacaag atggacctgg
      421 tctgctggag cgaggagcgc ttcatcgaga ttcgcgacca gttcgaagag ttcctgccgc
      481 gcctcgaagt gaaggacgtc cagttcattc cgctgagcgc gctcaccggg gacaacgtcg
      541 tcgagccctc caagcacacc ccctggtatc ccggccccac gctgctgggc catctggaag
      601 cggtccacat tggcagcgac tggaacctca acggcttccg cctgcccgtc caatgggtca
      661 atcgcccgaa ccgcccgacc gatccccagt tgcacgactt ccgcgggttc agcggccaga
      721 tcgcgggcgg catcgtgcgc gtcggccaga aagtcatggt gctgccgggc gggctggtca
      781 gcaccgtcaa gcaaatctgg acctacgacg gcccgctcca ggaagcgttc tgcccgcaat
      841 ccatcactct cgtgctcgac cacgatattg acgtcagccg cggtgacatg atcatcggcc
      901 cggacaacct cccgggcgcc agcaccaacc tgcgggccca agtctgctgg atgcacccgc
      961 gcgcgctcca gcctggccgg aagtactcgc tccagcacac gacaagaacg gtgcaggccg
     1021 tcatcaccgg catcgaagac cgcattgata tgaccacgct cgaacccgag cccaacccgc
     1081 ccgagttggc cctgaacgac atcggcgagg tccgcctcaa tacctccgcc ccgctcgtct
     1141 ttgacggcta cgctgcgaac cggctgaccg gctccttcat cctggtcgag ccgggcacca
     1201 atgcgaccgt cggggccggc ctgctcctcc cgcccaccga ggtggcgaaa ccggaataca
     1261 gtgattttgc gatctaatat tgcgaattgg caaggacatg gtcgaaggcg caccctctcc
     1321 catagggttg gcatcactca catctcccgc ctgcaagagg tgaatcccaa agggattaca
     1381 tcactcagcc cagggttgcg agccgcgagc taccctgggt gtgagtcaat taatgattta
     1441 ccaaccctga aagggttgca gcacctgacc cgaggggccg aggctcgtca agcacctgcg
     1501 ccgcgctaca accctttcag ggttgaacac gcttcgggaa cccgacccag ggtagctcgc
     1561 ggctcgcaac cttgggctgg agggtgcaat ccctttggga ttgaggaacg actgccggac
     1621 ttgcggggaa agttaagctt ctgggcaaag agctggggtg agggggaagg gaggtctgca
     1681 atactcgaag ctatcctatg aagctggatg tgaatagttc acccacagga ccgtgcagct
     1741 ctaagccaga cacagcttca ggcgaacgcc ctggccatcc ccccccaacg tctggcggca
     1801 tcgtctatct cgtcggcgcc ggtcccggtg acgccgggct tttcacgttg cgcggcaaag
     1861 aggtaatgga gcgggccgaa gtggtgatct acgacggcct cgtcagccgc gaactgctgc
     1921 gtttcgcccc gccctccgcg gaaatcatct acggtggcaa acacgaccgc acgcgctgcg
     1981 tgtcccaggc cgagttgaat gcgctgctgc tggccaaggc gctcgcaggc aaacgcgtgg
     2041 tgcggctaaa gggcggcgat cccttcgtgt ttggccgcgg cggcgaggag gccgaggcgc
     2101 tttcggcggc aggcatccct ttcgaagtcg tgc
//
LOCUS       DATCGS010000558         2955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_493820, whole genome shotgun sequence.
ACCESSION   DATCGS010000558 DATCGS010000000
VERSION     DATCGS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493820"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VIL39_11480"
     CDS             <1..542
                     /locus_tag="VIL39_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5482095.1"
                     /translation="EQNRFKAPDVILVPVGDGVIIAGVFKAFYDLKATGLIAKLPRLV
                     CVQAERSAAIHHYIRCGKYQDARRPDTIADSISVSIPSNAHLARRAVLESRGFSITVT
                     DQEILRGQRLLAQKAGVFAEPAAAATVAALKKLRASELLGRKEQIVLLITGHGLKDVE
                     AAMKTIRIPPSIEPTIDAL"
     gene            complement(621..2129)
                     /locus_tag="VIL39_11485"
     CDS             complement(621..2129)
                     /locus_tag="VIL39_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY5482096.1"
                     /translation="MKTALALIATCGTILLECPGQEPPQSSSIQGTSPGAKAPLIIPQ
                     PKSLRPGLGEFHVQPGAKWVLRGAARDERLWQAAQSVFAGKAGAFEKAGQPWVTLEIG
                     SSSRTNFPAEQTQPRWASDPEGYQLTVETNGLVILAATAQGAFYGLQTLAQLWQESGN
                     ESFCPALQIEDWPAMRFRGVHWFPSASGVPMHQKLIPNVFGAFKFNRSVIQCEAARWD
                     KHPEISARNAISKTDLRKLVETCRACYLEPIPLINMPGHAEWMFRDGNNLDFAEDPQT
                     PYAYCVNNPKSFEFIQDVLNECLPIFQSKFCHIGHDEVTMRGRFPNPECPRCKDATIT
                     TLVVKHVNRLSEWLSRRGIESMIWGDMLLGPGEAKDATHAKTLADARQRRAEIDKKTI
                     IVDWHYVASADARSIEVLRHDGFRVVAASWYTPQNIFQLSQAALTAGAEGLLQTTWMG
                     YFPDEQAMKTQLQQFTAFVLAAEYAWSGRKESPSQLGYDPKEVFLKAYARSR"
     gene            complement(2168..2590)
                     /locus_tag="VIL39_11490"
     CDS             complement(2168..2590)
                     /locus_tag="VIL39_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5482097.1"
                     /translation="MSLYKTILVTLEGTPTDRAIIEHIKVLAPSMQSRVVLLHVATGV
                     PAKYHRTDAAGKEIEESKAFLSRARDEFSAAGIPAEAQLAYGVPAKEIVNWVKKNGCD
                     LVAMSTHGHHMIADLVLGDTAIRVQHSLSIPVLLLRAK"
     gene            complement(2628..>2955)
                     /locus_tag="VIL39_11495"
     CDS             complement(2628..>2955)
                     /locus_tag="VIL39_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY5482098.1"
                     /translation="MRWRIRPWIRRMISRTIAIVPAVWIVSIRGTGSVTDLVNLSQVI
                     LCLILPLAMFPLLHFTSSRRRMGRWVNGWFLLILGWGSVLLITAMDLYSLPESLKEAW
                     RIITGG"
BASE COUNT          552 a          878 c          932 g          593 t
ORIGIN      
        1 acgagcagaa caggttcaag gcgccggatg tgattctcgt gccggtcggg gacggcgtga
       61 ttatcgccgg cgtcttcaag gccttttacg atttgaaggc gacggggttg attgccaaat
      121 tgcctcggtt ggtctgtgtc caggcggagc gttccgcggc catccatcat tatattcggt
      181 gcgggaagta ccaggatgcc cggcggcccg acaccatcgc ggactcgatt tccgtctcca
      241 ttccctccaa tgcgcacctg gcccggaggg cagtgctcga aagccgggga ttctcaatca
      301 ccgtcacgga ccaggagatt ctgcgcggac agagactcct ggcgcaaaag gccggtgtat
      361 tcgcggagcc ggcggcggcc gcgacggtgg cggcgctgaa gaaactgcgg gcatcagaac
      421 tgcttggccg caaagaacaa atcgtgctgc tcatcacggg ccatggcctg aaggatgttg
      481 aggcggccat gaagaccatt cgcatacccc cttcgattga gccaacaatc gacgccctgt
      541 aacccgcgcc cgatagtcat ccccaggctg gaagctggct gccgccgcaa cccgcaggca
      601 aggacgcctg cgctacagtt tcagcggctg cgagcgtagg ctttcaaaaa gacctccttc
      661 ggatcatagc cgagctggga gggactttcc ttgcggccgg accatgcata ttcggccgcg
      721 agcacaaagg cggtgaactg ctgaagctgg gttttcatgg cctgctcatc agggaaatag
      781 cccatccacg tggtttgcaa gagaccttcg gccccggcgg tcagggcggc ctgcgagagt
      841 tggaagatgt tctggggcgt gtaccaactc gccgccacga cgcgaaagcc atcgtgccgc
      901 agaacttcga tgctccgcgc gtcggctgac gccacgtaat gccaatcgac aattatggtc
      961 ttcttgtcta tctcggcccg tcgctgccgt gcgtcggcca gcgtcttggc gtgcgtcgca
     1021 tccttggcct cgccggggcc cagcagcatg tcgccccaga tcatgctttc gatccctcgc
     1081 cggctcagcc actcgctcag gcggttgaca tgtttcacca ccagagtggt gatcgtggca
     1141 tccttgcaac gcggacattc cggattaggg aagcggccgc gcatggtgac ttcgtcgtgg
     1201 ccgatgtggc agaacttcga ctggaatatc ggcaggcatt cgttcagcac gtcctggatg
     1261 aactcaaagc tcttcgggtt attgacgcaa taggcgtacg gggtctgggg gtcctcggca
     1321 aaatcaaggt tgttgccgtc acggaacatc cactcggcat ggccaggcat gttgatcagc
     1381 gggatgggtt cgaggtagca cgcacggcag gtctcgacga gcttccgcaa gtccgtcttg
     1441 gagatggcat tgcgggctga gatctccgga tgcttgtccc agcgggcggc ctcgcactgg
     1501 atcacgctac gattgaactt gaacgcgccg aacacattgg ggatgagctt ttgatgcatc
     1561 ggcacgccgg acgcgctggg aaaccagtgc acaccgcgaa agcgcatggc gggccagtct
     1621 tcaatttgga gggctgggca gaagctttcg tttccagact cctgccagag ctgggcgagc
     1681 gtttgcagac catagaaggc cccttgcgcc gttgccgcca ggatcaccag gccgttggtc
     1741 tcaaccgtga gctggtagcc ttcgggatca gaagcccagc ggggttgagt ctgctccgcg
     1801 ggaaaattcg tccggctgct ggaaccgatc tccagcgtca cccacggctg gccggccttt
     1861 tcaaaagcgc ccgctttgcc ggcgaacacc gattgcgccg cctgccatag ccgctcgtcg
     1921 cgcgcggcgc cacggagcac ccatttggcg ccgggttgca cgtggaactc tcccagcccc
     1981 gggcggaggg atttgggttg gggaatgatc aacggggctt tcgcgcccgg cgacgtcccc
     2041 tgaatcgaag atgattgcgg cggctcctgc cccgggcatt ccaagagaat tgttccgcag
     2101 gttgctatta aggcgagtgc tgttttcatg cgaagatcat tgggaactct ggaaacccgc
     2161 gcgtcgctca cttggcgcgg aggagcagga ccgggatgct gaggctatgc tgaactcgaa
     2221 tggcggtgtc gcccagcacc aggtcagcga tcatatggtg accgtgtgtg ctcatcgcca
     2281 ccaggtcaca gccgttcttc ttcacccagt ttacgatttc cttggctggc acgccgtagg
     2341 ccagttgcgc ttcggccggg ataccggcgg cgctgaactc gtcccgggcg cgactgagga
     2401 aggctttgct ctcctcgatt tccttgcccg cggcgtcggt gcgatggtac ttggccggca
     2461 cgccggtggc cacgtggagc aaaacgacgc ggctttgcat tgacggcgcc agcactttga
     2521 tgtgttcgat gatcgcccga tccgtcggcg tgccttccag cgtcacgaga atggttttat
     2581 acaggctcat ttgttgaatt tgtttcgggc ttcggattca tccgccctca tccgccggtg
     2641 atgatgcgcc aggcttcttt gagcgactcg gggagactat agaggtccat tgcggtaatc
     2701 aacagcacgc tgccccatcc tagaatcagc aggaaccagc cgttgaccca ccggcccatt
     2761 cgccggcgcg aggaggtaaa gtgaagcagc gggaacatcg ccagcggcag gatgaggcag
     2821 agaatcacct ggctgaggtt gaccaggtcg gtgacgctgc ccgtgccgcg gatgctgaca
     2881 atccagaccg ccggcacaat ggcgatcgtg cgggaaatca tccgccggat ccatgggcga
     2941 atgcgccagc gcatg
//
LOCUS       DATCGS010000559         4927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_345287, whole genome shotgun sequence.
ACCESSION   DATCGS010000559 DATCGS010000000
VERSION     DATCGS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345287"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VIL39_11500"
     CDS             complement(<1..361)
                     /locus_tag="VIL39_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482099.1"
                     /translation="MQNGIVLFLGALRHGEFESLLRRGIPCGALIDSNTKLHLPDLAQ
                     FQVVERFDFLRPEAELLHKLREIQAKWGLRCLINVVEHYVAVSARVAPQLGVAGISLR
                     SAQLCLDKLAMRQHFLER"
     gene            complement(401..2188)
                     /gene="ggt"
                     /locus_tag="VIL39_11505"
     CDS             complement(401..2188)
                     /gene="ggt"
                     /locus_tag="VIL39_11505"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5482100.1"
                     /translation="MAFPAASQTPELVPDMNACSRMPFLAWLTLVLALMQGCAGPGAQ
                     TGTSPLAPSGKYPSQSIERQNGVVVSVSGLASDVGVSILKQGGNAVDAAVATAFALAV
                     TYPAAGNLGGGGFMLVHPAPGEGKPVAIVYRETAPAAAFPTMFTKEDTQYMYKSVAVP
                     GTVRGFGLAHQRFGTLPWRQLLLPAVALARDGFKIDRNLAELMNTYLAAVPTHAEFQR
                     VFGKPGGGLWAAGDRLRQPDLARTLQLLADLGPDAFYKGPIAKAIVAEMARGKGLITG
                     EDLVRYQAIECKPLTTRYRGAYDVYVPPPPSAGVCLLEELNMLETFDLKAWGRWSPTT
                     FHVMSETMRRANFDRARYLGDPAFVQIPVNLTAPEYGHQLASTIDLKKATRSKDLAAD
                     LPLAHEGGDTTHFSIIDRRGMAVANTYTLERLWGSRIVVKDMGFLLNNNMFGFNLFPG
                     ITDTNGLVGTAPNLIAPGKRPVSSQTPTIVAKDGRVKLITGSPGTRAIPNTMACLLVS
                     LLDYEMPIQKAIEAPRMSHEWFPDQMSFETPERYPELMKALNKLGHTVVRYGPRPQGD
                     AQTIWVKGPNRYVGVADKRRNDKATASGY"
     gene            complement(2313..3503)
                     /locus_tag="VIL39_11510"
     CDS             complement(2313..3503)
                     /locus_tag="VIL39_11510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5482101.1"
                     /translation="MLAERMKSFGGSQTSGMRNKAKQLRERGVNVANFAAGELDLPTS
                     PAIKQAVREAIDADRTQYTDTLGIRVLREGIAERVSRATGVPYSAEEVGVTAGAKQAL
                     YNAAMVLFDPGCEVIIPAPYWVTFPVQVTLAGATPVFLPTEHNHFQIDLAELAGRITP
                     RTRGLIINTPHNPTGVVYDRPTLEGIAALALKHDFMVIFDECYEHLVYPPYEHHNLVK
                     LVPEMKSHTVLINSFSKTYCMTGWRAGFLSAPKAIIKAAANLQGHVTSNPCNLAQYGA
                     LAALDKVNEPFLNNVREVLSARRECALKLVQQIPHVHCGVPQGAFYLFPDVSQLFGRS
                     YKGKPIADVDALAELLLEEAHVAVVAGSAFGADRHIRLSYAVATKDVEDGLIRMRQFV
                     EGLQ"
     gene            complement(3544..4557)
                     /locus_tag="VIL39_11515"
     CDS             complement(3544..4557)
                     /locus_tag="VIL39_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY5482102.1"
                     /translation="MRTANSESNGPMTLDSRSTPALEPDHAARLFDGRPQVGAGVYDG
                     LSALLAAKWHFDFLWVSSLCCSAAAGLPDAGIIGPEDILNVVRCVHRSVDLPIVVDLD
                     SGYGDPVKVFHVVEAMARAGAAALCIEDNPSSKRCSLYEGYARALVSTEEHAARVRAA
                     QAGAAKALTNTRIIARTEALVAGMGVGEALRRAGAYVEAGADAVFIQSLDSTGKEVLT
                     FGREWSRRTPLFIAPTRLPQITRTQFFAAGISHTMFANQGLRAAHAAMDRTFRILAQA
                     ESSQPVESEISTVAAVADLVGAQKVVELEAYLAGKPVPPVTAAAKKPARRPAETSPQL
                     TAI"
     gene            4747..>4927
                     /locus_tag="VIL39_11520"
     CDS             4747..>4927
                     /locus_tag="VIL39_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5482103.1"
                     /translation="MGGCVDLTNSNHLKYRAYIDGLRAIAVLAVLFFHADIGCRGGYV
                     GVDVFFVISGYLITGL"
BASE COUNT          975 a         1443 c         1541 g          968 t
ORIGIN      
        1 tccgttcgag aaagtgctgg cgcatggcca atttgtcgag gcagagctgg gccgagcgca
       61 aggaaatgcc ggcgaccccc agttggggcg ccactcgagc ggaaaccgcg acataatgct
      121 cgacgacgtt gatcaggcag cgcagacccc acttcgcctg gatctccctg agcttgtgaa
      181 gcaattctgc ttccggtcgc aggaaatcaa agcgctcgac cacctggaac tgggccaggt
      241 cgggaaggtg gagcttggtg ttgctatcaa tcagggcgcc acaaggaata cctcgcctca
      301 acaggctttc gaactcaccg tgcctgaggg caccgagaaa taacacgatt ccgttttgca
      361 taatttgtgc ccgcgcggcc gaattacggc cacgcgggag tcagtatccg gatgcggtcg
      421 ccttatcgtt gcggcgttta tcggccaccc cgacatagcg atttggcccc tttacccaga
      481 tggtttgggc atcgccttgc gggcgaggcc catagcgaac cacggtgtgg cccagcttgt
      541 tcaaagcctt catcagctca ggataccgct ccggggtttc aaaactcatt tgatccggaa
      601 accactcgtg cgacatgcgc ggcgcctcga tggccttctg aatcggcatt tcatagtcca
      661 gcaggctgac caaaaggcag gccatagtgt tgggaatggc ccgggtgccg ggacttccgg
      721 tgatgagttt gacccgccca tctttggcaa caatggttgg cgtttgcgag ctgaccggtc
      781 gtttgcccgg ggcaatgagg ttgggcgccg ttccgaccag tccattggtg tcggtgatgc
      841 ccggaaacaa attgaagccg aacatattgt tattgagcag gaatcccatg tccttaacca
      901 cgatgcgcga gccccagaga cgttccaagg tgtacgtgtt ggcgaccgcc atccctcgcc
      961 ggtcaatgat cgaaaagtgg gtggtatcgc ctccttcgtg agccagcggc agatcggcgg
     1021 cgaggtcttt gctgcgggta gctttcttga ggtcaatagt gcttgcgagt tggtggccgt
     1081 attcgggggc cgtcagattg acggggatct ggacaaaggc gggatcgcca agatagcggg
     1141 cacggtcaaa gttggcgcgg cgcatcgtct cagacatgac gtggaaggtt gtgggggacc
     1201 agcgacccca tgctttcaga tcgaaggtct ccaacatgtt aagctcttcc aacaagcata
     1261 cgcccgcact gggtggaggc gggacataaa cgtcataggc gccccgataa cgggtggtca
     1321 acggcttgca ctcgatggct tggtagcgga ccagatcctc ccccgtgatc agcccctttc
     1381 ctctggccat ttccgcaacg attgccttgg cgatgggtcc tttgtagaac gcatccgggc
     1441 ccaaatcggc cagcagttgc agcgtccgcg ccagatcggg ctgacgtaag cggtcgccag
     1501 ccgcccacaa gccaccgccc ggcttgccga agacgcgctg aaactccgca tgcgtgggta
     1561 ctgccgccag gtaggtgttc ataagctcgg ccaggttgcg gtcaatcttg aagccatccc
     1621 gagccagggc cacagccggg agcagcagtt gccgccacgg cagcgtgccg aatcgctggt
     1681 gcgccagccc gaagccacgg accgtcccgg gaacggcgac ggatttgtac atgtactggg
     1741 tgtcctcctt ggtgaacata gtcggaaaag ccgcagccgg tgcggtttca cggtaaacaa
     1801 tggccaccgg tttgccctcc ccgggcgcgg gatgaacgag catgaagccg ccaccgccca
     1861 ggttgccggc ggcgggataa gtgacggcca aggcgaaggc cgtagccacc gcggcatcca
     1921 cggcgttgcc gccctgcttg agaatggaca cgccgacatc cgaggccaga ccggaaaccg
     1981 agacgaccac gccattttgg cgctcaatag actggcttgg atacttgccg gacggcgcga
     2041 gagggctggt tccggtttgg gctcccgggc cggcgcagcc ttgcatcagc gccaggacca
     2101 aagtcagcca ggcgaggaac ggcattcgcg agcacgcatt catgtcggga actaactctg
     2161 gggtctggct ggccgctgga aaagccattt gtgagaaaac atccggcgcg tttctctgga
     2221 aacaggaatg ggcatccggc gcaacaagac tccttgccag ccttccaggg acgagaactg
     2281 ctgcccgctc gaaagggctg gtgctggccc tcttattgca ggccctccac aaattgccgc
     2341 atccggatca gcccatcctc aacgtctttc gtcgccaccg cataggaaag gcggatgtgc
     2401 cggtcggcgc cgaaggccga accagcgacc accgcgacat gagcttcctc aagcagcaac
     2461 tccgccaggg catcaacgtc agcaatgggt ttgcctttgt aacttctccc gaagagctgg
     2521 ctcacatcag ggaagaggta gaaggctccc tgagggacgc cgcagtggac atgcgggatt
     2581 tgctggacga gtttgagggc gcactcccgg cgcgcgctca ggacttcccg aacattgttc
     2641 aggaatggct cgttgacctt atcgagcgcg gccagcgcac catactgggc gagattgcag
     2701 ggattggagg tgacgtgtcc ctgaaggttg gccgccgctt tgatgatcgc cttgggagcg
     2761 ctgagaaagc cggcccgcca tccggtcatg caatacgtct tggaaaagga gttgatgagc
     2821 acagtgtggc tcttcatctc cggcacaagc ttcacgaggt tgtggtgttc gtaaggcgga
     2881 tacaccaagt gctcgtaaca ttcgtcaaag atcaccatga aatcatgttt cagggccagg
     2941 gcggcaatgc cctccagcgt gggccggtcg taaaccacac cggtcgggtt gtgcggtgtg
     3001 ttgatgatga gacctcgcgt ccggggggtg atccgccccg ccagttctgc caggtcaatc
     3061 tggaagtgat tgtgctcagt gggcaggaaa accggggtcg ccccggccaa ggtcacctgc
     3121 actgggaagg taacccagta tggggcgggg atgatgacct cgcagcccgg atcgaacagc
     3181 accatggcgg cattatagag ggcctgcttg gcgcccgctg tgacgcccac ttcttcggcc
     3241 gagtagggaa ccccggtggc tcgggaaacg cgctcggcga tcccttcccg caaaacgcgg
     3301 atgcccaggg tgtccgtgta ctgggtacgg tcggcgtcaa tggcctcccg gacagcctgc
     3361 ttgatggcgg ggctggttgg caggtcaagt tctccggcgg cgaaattggc cacgttgacg
     3421 ccgcgctcgc gcagttgctt ggccttgttg cgcatgcccg aggtctgcga accgccaaag
     3481 ctcttcatcc gttcagctaa catatcgtgg ctccaattcg gtctaagctg tgatttgcgc
     3541 agcttagatg gcggtgagtt ggggcgaggt ttccgccggc cgtcgagcgg gttttttcgc
     3601 ggctgcggtg accggcggca caggctttcc agccagataa gcttccaact caaccacctt
     3661 ttgcgccccc actagatcgg ccaccgccgc aacggttgaa atctcagact ccaccggctg
     3721 agaagactcg gcctgcgcca ggatccggaa ggtgcgatcc atggcggcgt gggcagcgcg
     3781 caggccctga ttggcgaaca tggtgtgcga gattccggcg gcgaagaact gtgtcctggt
     3841 gatttggggt agccgcgtgg gggcgatgaa gagtggggtg cggcggctcc attcgcggcc
     3901 gaacgtgagc acttctttgc cggtggagtc gagggactgg atgaaaacgg catccgctcc
     3961 cgcctcaacg taagctcccg cccgccgcag agcctcgccg acgcccatac ctgctaccag
     4021 cgcctcggtc cgggcgatga tccgggtgtt agtcagggct ttggccgctc cggcctgcgc
     4081 ggcccgcacg cgggcggcgt gttcttcggt ggaaaccagg gcgcgagcat aaccttcata
     4141 gagactgcag cgtttggagc tggggttgtc ctcgatacac agggccgccg cgccggcgcg
     4201 agccatggct tcgaccacat ggaaaacctt caccggatcg ccataaccgg agtcgagatc
     4261 aaccactatg ggcaagtcca cagatcggtg aacacatcgg accacgttca ggatatcttc
     4321 gggaccgatg atgccggcat caggcaaccc cgccgcggcg gagcagcaga ggctgctgac
     4381 ccataaaaag tcaaaatgcc acttcgcagc cagcagcgcc gagagcccgt cgtacacccc
     4441 ggcgcccacc tggggacggc cgtcgaacag gcgcgcagca tggtccggct caagcgctgg
     4501 cgttgaacgg ctgtcaaggg tcatcgggcc gttactctct gaatttgctg ttcgcataat
     4561 agtctgttta gtctgttccg gcagaatcgc cagccattca tccagtccac cagaccagca
     4621 tgacagagtg atgagccgcc gatgacagtt tcgtcatttt gaatacgcct ctgacgacat
     4681 cggcaatcgg aagacggctg gccggggtgg agatagtgct tgtattctaa attccgcgac
     4741 cattttatgg gaggctgtgt ggatttaacg aacagtaacc atctcaaata tcgggcctat
     4801 attgatggcc tcagagccat cgccgtcctg gcggtcctgt tctttcacgc cgacattgga
     4861 tgccgtggag ggtatgtcgg cgtagatgtc ttcttcgtta tttccggcta cctcatcact
     4921 ggtctaa
//
LOCUS       DATCGS010000560         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_97177, whole genome shotgun sequence.
ACCESSION   DATCGS010000560 DATCGS010000000
VERSION     DATCGS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97177"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1559
                     /gene="ltrA"
                     /locus_tag="VIL39_11525"
     CDS             183..1559
                     /gene="ltrA"
                     /locus_tag="VIL39_11525"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEY5482104.1"
                     /translation="MRQKTEMEITLHGGATGEARSADAEGTEARAATTEIESRAAAEP
                     SMEAIVERDNLRKALAQVKRNKGAAGVDGMSLGALAPYLKEHWPTIRAQLLDGTYKPQ
                     PVRRVEISKATGGTRLLGMPTVLDRFIQQAVLQVLQVNWDRTFSEHSFGFRPKRSAHQ
                     AVKRAQELIASGHGVVVDIDLEKFFDRVNHDILMGLVAKRVTDKRILVLIRGFLTAGA
                     LAGGLVSTTEEGTPQGGPLSPLLSNLMLDVLDRELETRELNFVRYADDCNIYVRSQRA
                     GERVMASVERFLAKRLKLKINKAKSAVARPNKRKFLGFSFTNGKQPRRCIAPQSLARF
                     RSRVRELTRRNRGKSLAQIVKELSVYLIGWRGYFGFCETPSELRDLDKWIRRRHRAIV
                     WKQWKRGPARFAELRRRGVGRDLAARSAGSPHGPWRLSMSPALTIALSNAFFTSLGLA
                     SLVDTQPT"
     gene            1640..1915
                     /locus_tag="VIL39_11530"
     CDS             1640..1915
                     /locus_tag="VIL39_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482105.1"
                     /translation="MFALLTSVDWKLVRLEHLEETMNIPGLTNDALMALHQLIAEAQA
                     ADDAAAAAERRRPFGVREYPDWRKQAGMYEAEMKKRGLVFEHIAWAK"
     gene            2615..2860
                     /locus_tag="VIL39_11535"
     CDS             2615..2860
                     /locus_tag="VIL39_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482106.1"
                     /translation="MRESTSAELMAWCTGALRQGANFQDVWENLLKGNPLVCGEPRQR
                     VEESHPHMEVFLATGERLVFDIERHEFSLEGMSTLNH"
BASE COUNT          655 a          834 c          985 g          579 t
ORIGIN      
        1 cgtgcggtga aggcggtcga tcttacctcg ggaggtcttc ggcgcgtccg ggagaccgga
       61 ctgagcggat cgcaaggtcc gtgatcgcgg cggagaagtc agcagagggc ataataggtg
      121 gagcaatcca ccgaaggccc aaacgttgga atggcaagta gaacggcgat ctcgcgtggg
      181 ccatgcggca gaaaactgag atggagatca ccttgcacgg cggagcgacg ggtgaagccc
      241 gaagtgccga cgcggaaggg accgaagccc gagcggcgac aaccgaaatc gaaagccggg
      301 cggctgctga gccgtcgatg gaagcgattg tcgaacgaga caatctgagg aaggcgctgg
      361 cgcaagttaa gcgcaacaaa ggagcggccg gcgtcgatgg catgagcctc ggcgcgctgg
      421 ctccttacct gaaggagcac tggcctacga tccgggcaca gttgcttgac ggcacctaca
      481 agccgcagcc ggtacggcgc gtggagatat cgaaggcgac gggcggcaca cggctgctcg
      541 gcatgccgac ggtactcgac cgtttcatcc agcaagcggt gctgcaagtg ctgcaagtca
      601 attgggaccg aacgttctcc gagcatagct tcggctttcg gccgaaacgc tcggcgcatc
      661 aggcggtgaa gcgggcgcaa gaattgatcg cgtccggtca cggcgttgtg gtggatatcg
      721 acctggagaa attctttgac cgggtcaacc acgacatcct gatggggctg gtggcgaagc
      781 gggtgactga caagcgaatc ctggtgttga tccgcggctt tttgactgcg ggtgcactag
      841 caggtgggct ggtcagcacg acggaggagg gcacaccgca aggtgggccg ctctcgccgc
      901 tgttgtcgaa cctgatgctt gatgtgctgg acagggaact ggaaacgcgc gagctcaact
      961 tcgtgcgcta tgccgatgac tgcaacattt acgtgcgcag ccaacgagcc ggcgagcggg
     1021 tgatggcgag cgtagaaaga ttcttggcca agcgtctcaa actcaagatc aacaaggcga
     1081 agagtgcggt cgctcgcccg aacaagcgta agttcctggg cttcagcttc acgaatggga
     1141 aacagcctcg gcggtgtatt gcaccgcagt cgcttgcccg attccgaagc cgggtgcggg
     1201 aactgacgcg tcgtaatcga ggcaagagcc tcgcgcagat cgtcaaagag ctgtctgtct
     1261 atctcatcgg atggcgcggc tacttcggat tctgcgaaac cccgtcggag ttgcgcgacc
     1321 tcgacaagtg gatcagacga cggcatcggg ccatcgtctg gaagcaatgg aaacgcgggc
     1381 ctgcccgctt tgccgaactg cgacgccgcg gcgtcggccg ggacttggcg gcgcggagcg
     1441 ccggcagccc acatggccct tggcggctca gcatgagccc cgcgctcacc atcgccctat
     1501 ccaatgcctt cttcacctcg ctcggcctag cttctctcgt ggatactcag ccgacttaat
     1561 ccatcgaacc gccgtgtacg gacccgtacg cacggtggtg tgggagggga ggagccgcga
     1621 ggctcccccc tatcccgatt tgttcgccct tttaacttca gttgattgga aattagtccg
     1681 gttggagcat ctggaggaaa caatgaatat tccaggtctc actaacgacg cgttgatggc
     1741 cttgcaccag ttgattgcag aggcgcaagc agctgatgat gccgccgccg ccgctgaaag
     1801 gcggaggccg ttcggtgtaa gagagtaccc cgattggcgc aaacaggctg gtatgtacga
     1861 ggccgaaatg aaaaagcgag gcctcgtctt cgagcatatt gcatgggcga aatagacttt
     1921 tccatttgcc atcggcccgg gccaccgaag aaagggctca agagcaattc ccttggtgag
     1981 gtagggaagc ggtcattccc tcggagccgg caggttcaat cgcgcgacca cggccagcgg
     2041 ggtcaaggtt gcgcagcacc cgcagggctt gaccttgacg ccagtaccag gcggcggcgc
     2101 acgatggcgg aaggttaaac cgaggctcgc ggtaagaccg tctgcgcgac ttgctgctga
     2161 aaactggggt cgcaatccgc ggcgccggaa tcgcacaccc acgcatcctt ggcgaaccat
     2221 tcgtgtgtca tccgacacaa tgggcaatat gtgcggctga agaatacggg ggtggagtgg
     2281 aaggttgcgc ggtcaacctg catgccagtg gaaatggctc gccgtgtctt ggggcaacgg
     2341 atcatcacta tacccatttg gcggctcctt cttctgcgct cgccctcatc aagtacagtg
     2401 cttgtgcgac agaaacataa agggacggtg acaattccga ggcattgtca gggcactcga
     2461 tcagggcgga ttttaatttc agcagccacc gcggcagctc gcgcggcgtg ttcgccggca
     2521 tcgtcgagat cgcagtggtc ggcttgccct ggtcacgggc atgcgtttga tgtatgtcaa
     2581 cgcgccattt attgcactgc accaatatat cactatgcgt gaatctacct cggccgagtt
     2641 gatggcctgg tgcaccggcg ctctgcgcca gggagcgaat ttccaggatg tgtgggagaa
     2701 cctcctcaag gggaatccgc tcgtgtgcgg ggaacccaga caacgggtgg aggaatctca
     2761 cccgcatatg gaagtcttcc tcgcgacggg ggagcgccta gtcttcgata tcgagaggca
     2821 cgaatttagc ctggaaggga tgtcaacgct aaatcattaa tttcgtctga gcgtagattc
     2881 gaaaggtttg atggaaatgt atcgctttct ggaatccacg gccggtcaat atatgacgcc
     2941 ggccgtcaag agcctgaccc gcgactgcac catgagcgag gcggaaaaat tgtttgacca
     3001 gcatgactac aatgcctttc cggtcgtgga agatggcaag ctgctgggta tcg
//
LOCUS       DATCGS010000561         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_97254, whole genome shotgun sequence.
ACCESSION   DATCGS010000561 DATCGS010000000
VERSION     DATCGS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97254"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>2738
                     /locus_tag="VIL39_11540"
     CDS             168..>2738
                     /locus_tag="VIL39_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEY5482107.1"
                     /translation="MATSEFVIYLGELSSNLKHGDATEHTHRPALKALLEAIGTDIVA
                     TNEPTRILCGAPDFNITRGNVPLGHVETKDIGENLDLMERGKPPYGEQFGRYRDGLPN
                     WILTDYLEFRWYVNGEHRLTARLAELDGKGKVKAVPGGAEAVEELLGAFLQQKALTIG
                     TAKDLAQRMAGMTRIIRELIIKTFEHEVEKGLLHNWLAGFRETLIPDLDEKQFADMFA
                     QTLAYGLFAARIHAPPKEEFTRKSAAYCIPKTNPFLRKLFSEIAGVDMPETIAWAVDD
                     MVELLKHADMFSILKDFGKGQGKEDPVVHFYETFLAAYDPKMRQLRGVFTTPEPAVGY
                     IVRSIDYLLRARFNRPKGLADENTLVLDPATGTATFLYFVISLIYQKFARQKGAWDGY
                     VEKHLLNRIFGFELLMAPYAIAHLKLGLLLQETGYTFGSDQRLGVFLTNTLEEAARKS
                     EHLIANWISEEANAAAKIKRDDPIVVVLGNPPYSNFGQMNRGEWILRLLRDYKQGLNE
                     TKLNLDDDFIKFIRFAQWRIERSGEGIIGFITSNTYLDGLTHRRMRECLLTAFNEIYV
                     LNLHGSSKKQEAAPDGSKDENVFDITVGVSIVFFVKLPGAAPTGANACRVFHADLWGS
                     RESKYKALLETDAASTPWTELKPASPHFYLVPKDFKQAAEYRQGWSLREAFLVSGNAI
                     KTERDRVSIHFTKKGIQGAVEDFRQLDEPALRQKFDLEQDSRDWKVASAKADVLAHRG
                     PDCFRPILYRPFDIRFTWYSGQSKGFIGTPGHALMRHMLAGENLAFITTRQTAETFDL
                     LCTNCLAGHKSVAAYDINSVFPLYLYGDGHSEKSRQGGGGTMLMALFEPAAAYSARRA
                     N"
BASE COUNT          584 a          848 c          778 g          528 t
ORIGIN      
        1 cgcctgaagc ctactggcgg tatctggcgg atcggaacga ttcgcccggg atgcggctgt
       61 ctgtcggcgc agcctctggc gaagggcctt ggcttatcag aagcatggaa taaagcggct
      121 ttccgccctt gtcgagttgc aggttgagcc gttagagtga ctgcgccatg gccacatctg
      181 agtttgtgat ctacctcggc gagttgagct cgaacctgaa gcacggggac gccaccgagc
      241 acacccatcg cccggcgctc aaagcgctgc ttgaggccat cggcacggac atcgtcgcta
      301 cgaacgagcc aacgcgcatc ctgtgcggcg cgcccgattt caatatcacc aggggcaacg
      361 tgccgctggg tcatgttgaa accaaggata ttggcgagaa cctcgacctg atggagcgtg
      421 gcaaaccgcc ctacggcgag caatttggcc ggtatcggga tggcctgccc aactggattc
      481 tgaccgacta cctggaattc cgctggtatg tgaatggcga gcaccggttg actgcgcggc
      541 tggccgagtt ggacggcaag ggaaaggtca aggccgtccc gggcggtgcg gaagcagtcg
      601 aggagcttct cggggcgttt ctccagcaga aggccctgac catcggcaca gcgaaggacc
      661 tggcccagcg catggcgggc atgacccgca ttatccgcga gctgatcatc aagaccttcg
      721 agcatgaagt cgagaagggc ctcctccaca attggctcgc tggcttccgc gaaacgctaa
      781 tccccgacct ggacgaaaag cagttcgccg atatgttcgc gcagacgctt gcctacggcc
      841 tgttcgccgc gcgcatccat gctccgccaa aggaagagtt cacgcggaag tccgccgcgt
      901 attgcatccc caagaccaat ccgttcctcc gcaaactctt ctccgaaatt gccggcgttg
      961 atatgccgga aaccatcgcc tgggcggttg acgacatggt cgaactgctc aagcacgccg
     1021 acatgttctc tatcctgaaa gacttcggaa agggccaggg caaggaagac cccgtcgtcc
     1081 atttctacga aacctttctc gccgcctacg acccgaagat gcggcaactt cgcggggtct
     1141 ttaccacgcc cgaacctgcg gtcggctaca tcgtccgctc cattgactac ctgctgcgcg
     1201 cccggttcaa ccgcccgaaa ggtctggccg acgaaaacac gctcgtcctc gaccccgcca
     1261 ctggcacggc cactttcctt tactttgtca ttagcctcat ctaccagaaa tttgccaggc
     1321 aaaagggagc gtgggacggg tatgtggaaa agcacctcct gaatcgcatc ttcggtttcg
     1381 aactgctcat ggcgccctac gccatcgcgc acctcaagct cggcctgcta ttgcaggaga
     1441 ccggctacac gtttggctct gaccagcggt tgggggtctt cctgaccaac acgcttgaag
     1501 aggcagccag gaagagcgaa cacctcatcg ccaactggat ttccgaggaa gccaatgccg
     1561 ccgccaagat caaacgggac gatcccatcg tggtggtgct cggcaatccg ccctattcca
     1621 acttcggtca aatgaaccgc ggcgagtgga ttctgcggtt gctgcgagac tacaagcagg
     1681 gtttgaacga gaccaagctg aacctcgatg acgacttcat caagttcatc cgctttgctc
     1741 aatggcgcat cgagcgctcg ggcgaaggca tcatcggctt catcaccagc aacacctatc
     1801 ttgacggact tacgcatcgc cggatgcgcg aatgcctgct gaccgcgttc aatgagattt
     1861 acgtattgaa cctccacggc agctcgaaga agcaggaggc cgcacccgat ggctcgaagg
     1921 acgagaacgt ttttgacatc actgtgggcg tctcgattgt gttcttcgtc aagctgcctg
     1981 gggccgcgcc gaccggggcc aacgcttgtc gggtcttcca cgccgacctt tggggcagcc
     2041 gggaaagcaa atacaaagcc cttttggaaa ccgatgccgc cagcacgccg tggacggaac
     2101 tcaagcccgc ctcgccccac ttttacctcg tgcccaagga tttcaagcaa gcagcagagt
     2161 accggcaggg atggagcttg cgcgaagcct ttctcgtgtc cggcaacgcc attaagacgg
     2221 aacgcgaccg ggtctcgatt cacttcacca agaagggcat ccagggggcg gtcgaagatt
     2281 tcaggcaact cgacgagccc gctctccgcc agaagttcga cctggagcaa gacagccgcg
     2341 actggaaagt tgcgagcgca aaagcggacg tgcttgccca ccgtggtcca gattgcttcc
     2401 ggccaatcct ctatcggccg ttcgacattc gcttcacctg gtacagtggc cagtcgaagg
     2461 gctttatcgg cacaccaggc cacgctctta tgcgccacat gctggcgggc gagaaccttg
     2521 cctttatcac cactcgacaa acggcagaga ccttcgattt gctctgcaca aactgcttgg
     2581 ccgggcacaa gtctgtcgcc gcctatgaca tcaacagtgt ctttccactt tacttgtatg
     2641 gcgacggcca cagtgaaaag tccagacaag gtggcggcgg cacgatgctc atggcgctgt
     2701 ttgaacccgc tgccgcgtac agcgcgcggc gcgcaaac
//
LOCUS       DATCGS010000562         6404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_691729, whole genome shotgun sequence.
ACCESSION   DATCGS010000562 DATCGS010000000
VERSION     DATCGS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691729"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VIL39_11545"
     CDS             <1..844
                     /locus_tag="VIL39_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY5482108.1"
                     /translation="RQHYGITDDFLKRAREAFAAEVQLVDFGSRSDALLGQINSWVSR
                     QTHGKIENALLPGSISTDMPLVFVNTLFFKGQWASRFPMQHTQPRRFWLSPDHSVRVK
                     MMEQTCHARYAESDLAQMVELPYAGFGLSMIVLLPRQPQGLPELEQQLSENLLTNWMA
                     AARGHTVDLQLSRFSIRSRLPLSQPLSGLGMPDAFDRAKADFTGMTPQRPLWLAFLQQ
                     CAVVEVNEEGTVAAAATSGGFACSSQPPPATFHADHPFLFFIRDNRTGVILFLGRVIN
                     PAQS"
     gene            complement(1040..1183)
                     /locus_tag="VIL39_11550"
     CDS             complement(1040..1183)
                     /locus_tag="VIL39_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HEY5482109.1"
                     /translation="MDVDAYVTNKALDGLFIMVAEDEKRIRENPAARTTVLLQQVFGA
                     VAK"
     gene            complement(1188..2600)
                     /locus_tag="VIL39_11555"
     CDS             complement(1188..2600)
                     /locus_tag="VIL39_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482110.1"
                     /translation="MELRDSQSKGRTPRVFVFNPFAEGHIAHGKSFTPGKHQTILASD
                     LANLPQFLCRSDDIVLVAKRPSVEFLSAVRQAGFPLPEFVELKDGRIDPASNLCQRKI
                     GSLRPWAWGPDSLELLEPLLAQVTGEARTASQRFNEGIARLYSKAWSADFLRTVLARN
                     RRGDAAHPPGVESQSLPAPAAEEPWLCTEDEAGVAVDTLEGALEAIAAIRRRGHHRVV
                     AKEAHGLAGHNAIRLWEPELLAAQQQWLARALQNGRQLVVEPWLERELDFSIQLEMGP
                     RSLRLCGYTGLINDRKGQFQANCAAANCDRRIPANVAALFREPADISGRLQRLYGDIL
                     SLLGAELQRAGFVGPVGIDAFVYRTPQGGCRLKPVVEINPRYTMGRLTLELMKQTCPG
                     SSGLFRLVSRAQARVEGFADFAGYAGSLSERFPLRLEGEPVPKIREGALSLNDPAEAQ
                     VCLATFQVSAEGNPSALPED"
     gene            2798..5065
                     /locus_tag="VIL39_11560"
     CDS             2798..5065
                     /locus_tag="VIL39_11560"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482111.1"
                     /translation="MKRLLFGLGLLSGLVSANSLAQAPRWQPIGISGGGGMFAPAISP
                     ANPNLMMLNCDMSAAYISEDGGRNWRMIHQAQLRSDTACRPAFHPTDPNILYASSGGR
                     LKISRDRGKTFAPIGNLKESLGGEITINPSDPKTILAGSRSGRCWLSRDAGETWAACP
                     GPAGQVIAFHFDRTRQGRSMFAATDKGIWRSDDDGQNWSEKTQGLPWKQIQGFAGGSD
                     AAKKLVLLYCSIRSQDESGVFKGGLYRSRDRGETWESAMGQGLNTETKKADEYAYGSV
                     AQYHQLLATDAKPLTVYALNTSTGFHPPHSDTVYRSDDGGETWRATYFQDPRFKSYNV
                     APDWETASCGQCFKGGETPFGVAICNADPDRVILVRNEPHITHDGGATWFGGHTYPAP
                     GQKPGPGSAWVCNGLVVTTTWHFYIDPSEPNHHYICYTDIGMARSADAGQTWIWWNPK
                     TWAPWRNTCYEMAFDPETPGKIWGAFSDVHDIPNDNIISERHGHGRPGGVCLSRDFGA
                     SWKSETEGLPRKPVTSIVLDPRSPKNARTLYAGVFDEGVFKSTDDGKTWALKKTGLGH
                     PDNLRVYRVILHRDSTLFAIICARRPASAQPLMPEGVGLYRSKDGAETWEKINASKPL
                     LYLKDFSVDPRNSRRILLGAADAGRGDQSGGLYLTEDGGQNWERIGREGPQTFGGYFH
                     PKHAGWIYMTLTEGAPGAGLWLSRDNGKTWRAFDDLPFSNIQRVEFDPANDAVIHVTT
                     FGGSVWRGPATPSEP"
     gene            5544..6329
                     /gene="nagB"
                     /locus_tag="VIL39_11565"
     CDS             5544..6329
                     /gene="nagB"
                     /locus_tag="VIL39_11565"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEY5482112.1"
                     /translation="MEVIIQPNQEAAASLVARIVARELRTNPHLVLGLATGQTMECVY
                     RHLVRLHQIEKLDFSLCRTFNLDEYVGLFPADPNSYRHFMNHNLFSQVNIDPRNTHLP
                     NGLATDLDEECRRYEATIKRCGGIDLQVLGIGKDGHIGFNEPLSALRSRTRVKALTPT
                     TLKQNAPFFGGEDKMPHRAITMGVGTIQEARRCVLLATGASKANVIARAVEGPITSMI
                     SASALQMHPRATVIVDEEAAGNLIEKEYYRWIFANEPEWEEYR"
BASE COUNT         1226 a         2126 c         1902 g         1150 t
ORIGIN      
        1 gcggcagcac tacggtatta ccgatgactt tctcaaacgc gcgcgcgaag ccttcgctgc
       61 cgaggtccag cttgtcgatt tcggctcccg ctccgacgcg ttgctcggcc agatcaattc
      121 ctgggtgagc cgccagactc acggcaagat cgaaaatgct ctcttgccgg gaagcatctc
      181 taccgacatg ccgctggtgt tcgtgaacac gctcttcttc aaaggccaat gggcctcccg
      241 attccccatg cagcacacgc agccgcggcg cttctggctt tcgccggacc actccgtccg
      301 cgtgaaaatg atggagcaga cgtgccatgc ccgttacgcg gagtcggacc tggcgcagat
      361 ggtcgagctg ccttacgccg gcttcggcct gtccatgatc gtgctgttac cccggcagcc
      421 ccagggcttg ccggaactgg agcagcagct ctccgaaaac ctcctcacga actggatggc
      481 cgcggcgcgc ggacacaccg tggacctcca actgtcccgg tttagcatcc gctcccggct
      541 cccgctcagc cagccgctgt ccgggttggg catgcccgac gcatttgatc gggcaaaagc
      601 agacttcacc ggcatgaccc cccagcgccc cctgtggctt gcctttctcc agcagtgtgc
      661 cgtcgtggag gtcaatgaag agggcacggt tgccgccgcc gcgaccagcg gcggcttcgc
      721 gtgcagttcc caaccaccgc ctgccacctt ccatgccgac cacccgttcc tgttctttat
      781 ccgcgataac cgcaccgggg taatcctgtt ccttggccgc gtgatcaatc cggcacaatc
      841 ctgagccgtt ggcatccctc ccctcgtccc ggccgtaggg cgtatcctgt ccgtaacaac
      901 ggggtgcgcc cgtcctcggg cgcagcgacg cccgcaggca gtggggtctc ggaacattcc
      961 agcgttgcgg gcgagtcgga agctgctgcg cccgaggacg ggcgcactcc ggcccaatca
     1021 ccttcagtgg tcaagcgcgc tactttgcga ccgccccgaa aacctgctgc agcaacacgg
     1081 ttgttcgagc agccgggttt tcgcgaatgc gcttctcatc ctccgccacc atgatgaaca
     1141 ggccgtccaa ggccttgttg gtgacatagg cgtccacgtc catggaatca gtcctccggc
     1201 agagccgaag ggttcccttc tgcactgacc tggaaggtcg cgaggcacac ttgcgcctcc
     1261 gcggggtcat tcaaggagag ggcgccttcc cggattttgg gcacgggctc gccttcgagg
     1321 cgcagtggaa aacgctcgct cagagaaccg gcgtagccgg caaaatccgc aaagccttcc
     1381 acgcgcgcct gcgcccggct aaccaggcgg aacaggccac tgctccccgg acaagtttgt
     1441 ttcatcagct ccagggtcag gcggcccatg gtgtagcgcg gattgatttc cacgaccggc
     1501 ttgagccggc agccgccctg gggtgtgcga tagacgaagg cgtcgatgcc gaccggcccg
     1561 acaaagccgg cgcgttgcag ttccgctccg agcagcgaaa ggatgtcgcc atagaggcgc
     1621 tggagccggc ccgaaatgtc cgccggttcg cggaacaacg cggccacgtt cgcaggtatg
     1681 cggcggtcgc agttggccgc tgcgcagttg gcttggaact gtcccttgcg gtcattgatc
     1741 aagccggtat agccgcagag cctcaggctg cgcggcccca tctccaactg gatggagaaa
     1801 tccaactcgc gctccagcca gggttcgacg accaattgcc ggccattctg caaggcacgg
     1861 gccagccatt gctgctgtgc cgccaacagc tccggttccc acagccgaat ggcgttgtgg
     1921 cccgccaaac cgtgggcttc cttggccacg acccggtgat gaccgcgccg gcgaatggca
     1981 gcaatggctt cgagagcgcc ttcgagcgtg tccaccgcca cgccggcctc gtcctcggtg
     2041 caaagccagg gttcctcagc agccggcgca gggaggctct gactttccac tccaggcggg
     2101 tgagccgcgt cgcctcggcg gttgcgagcc agcacggttc tgagaaagtc ggcgctccaa
     2161 gccttggaat agaggcgggc gatgccttcg ttgaaacgct gactcgcggt gcgggcttcg
     2221 ccggtcacct gggccaagag cggttcgagc agttccaagc tgtccgggcc ccacgcccag
     2281 ggccgcaaac tgccgatctt ccgctgacag agattgctgg ccggatcaat gcgtccgtcc
     2341 tttagttcga cgaactccgg cagcggaaag cccgcttgcc taacggcgct caagaactcc
     2401 accgagggcc gtttcgccac cagcacgatg tcgtccgacc ggcagaggaa ctgcggcagg
     2461 ttggccaggt cactggcgag gatcgtctgg tgcttccccg gggtgaagct cttgccgtgg
     2521 gcgatgtgcc cctcggcgaa gggattaaac acaaaaaccc tgggggtgcg gcctttggac
     2581 tgtgaatcac gaagttccat tgctcgccaa ccatgttacc tgttgacggg taatcttgcc
     2641 agctctccgg gttgctccga agcaggggtt aatgagaata tcataccgaa tattgctgat
     2701 cgtcgcgctg atcgtcgcgc ttgccatttc gttgtgccga cggtacaaaa agacagggcg
     2761 agaagatgac aatctcatta gctttatgcg tccttatttg aagcgtcttt tgtttgggct
     2821 tggcctgctg agcggactcg tctcggccaa ctcactggcc caggcccctc gctggcaacc
     2881 catcggcatc tcgggtggcg gcggcatgtt cgcgcccgcg atttctccgg cgaacccgaa
     2941 cttgatgatg ctgaactgcg acatgagcgc cgcctacatc tccgaagacg gcggacgcaa
     3001 ctggcgcatg atccaccagg cccagcttcg cagcgatacg gcctgccgcc cggcttttca
     3061 cccgaccgat ccgaacatcc tctacgcctc ctccggtgga cggctcaaga tcagccgtga
     3121 ccggggcaag actttcgcgc ccatcggcaa tctcaaggag tctctgggcg gggagatcac
     3181 gatcaatcca tccgatccaa agaccattct ggccggctcg cgcagtggcc gctgctggct
     3241 ctcgcgggac gcgggagaga cgtgggcggc gtgcccgggg ccggcgggac aggtgatcgc
     3301 ctttcacttc gatcgcacca ggcagggccg ctcgatgttc gctgccaccg acaaaggaat
     3361 ctggcggtcc gacgacgacg ggcaaaactg gtcggagaaa acccagggcc tgccgtggaa
     3421 gcagattcaa ggtttcgccg gcggctcgga tgcggcaaag aagctcgtcc ttctctattg
     3481 ctccatccgc agccaggacg agagcggagt gttcaaaggc ggcctgtacc gctcgcgcga
     3541 ccgaggggag acgtgggaat cagcgatggg ccaaggcctc aataccgaga cgaagaaggc
     3601 cgacgagtac gcctacggtt ccgtggcgca ataccatcaa ctgctcgcga ccgacgcgaa
     3661 gccgctcacc gtctatgcgt taaacaccag caccggcttc cacccgccac acagcgacac
     3721 agtttatcgc agcgacgacg gcggcgaaac gtggcgcgca acttacttcc aggatccgcg
     3781 ctttaagagc tacaacgtgg caccggattg ggagaccgcc tcgtgcgggc aatgcttcaa
     3841 aggcggcgag acgccgttcg gcgtggccat ctgcaacgcc gatcccgacc gcgtgatcct
     3901 cgtgcgcaat gagccgcaca tcactcatga cgggggcgcg acgtggttcg gcgggcatac
     3961 ttatccggcg cccggccaga agcccggccc gggctccgcc tgggtctgca acggcctggt
     4021 ggtcaccacg acctggcatt tctacatcga cccgtccgaa ccgaaccacc actacatctg
     4081 ctacaccgac atcgggatgg cccgctccgc cgacgcgggc cagacgtgga tttggtggaa
     4141 cccgaagaca tgggcgccgt ggcggaacac ctgttacgag atggcgtttg atcccgagac
     4201 gcccggcaag atctggggcg cgttctccga cgtgcatgac atccccaacg acaacatcat
     4261 ctccgagcgc cacggccatg ggcgtcccgg gggcgtttgc ctctcgcgcg acttcggcgc
     4321 gtcgtggaaa agcgagacgg aaggacttcc gcgcaaaccg gtgacttcca tcgtgctcga
     4381 tcccaggagt ccgaagaacg cgcgcacgct ctatgccggc gtcttcgatg aaggcgtctt
     4441 caagtccacc gacgacggca agacgtgggc actcaagaag accggcctgg gccatcccga
     4501 caacctgcgc gtctatcgtg tgattctcca ccgcgacagc acactgttcg ccatcatctg
     4561 cgccaggcgg cccgcatccg cccaaccgct catgcccgaa ggcgtcggcc tgtatcgttc
     4621 caaggacggc gccgagacct gggagaagat caatgcctcc aagccgctgc tctacctcaa
     4681 agacttctcc gtcgatcccc gcaacagccg tcgaatcctg ttgggcgcag cagatgccgg
     4741 ccgcggcgac caatccggcg gcctctacct taccgaggac ggcggccaaa actgggagcg
     4801 catcggtcgc gaagggccgc agacgttcgg gggctatttc cacccgaagc atgccggctg
     4861 gatttacatg accctgaccg aaggcgcgcc cggcgcgggc ctctggctct cacgcgacaa
     4921 cgggaaaacc tggcgagcct tcgatgacct gcccttctca aacatccagc gggtcgagtt
     4981 cgatcccgcc aacgacgccg tgatccatgt gacaacgttc ggcggcagcg tgtggcgcgg
     5041 cccggccacg ccgtctgagc catgaaccaa ttctgccacc gggtcgtcgc gaacaagacc
     5101 tgttcggcaa aatcacgaag ttcccttgct cgccaaacaa gttagctgct gatgggtaag
     5161 ctggccagct ccccagattg ctccggacgc atctcgtacc acaaatgagc tggtcgtgaa
     5221 gtccatgaga atgtcgtatt tccgcccccg ccaaaaccat gaccctggat cgcaggttca
     5281 gcgggctcgt taaccacgcc cacggcccca ggcttgcgat cggcgggccg aagacagctt
     5341 tcacgggttg cggccagttt ggaaagcggg tcagcgatag ttggcactca gcggcggggc
     5401 aggactggcc gccatgcgga cgatccgcgt gatgatggac gataactacc cgccgtttgt
     5461 tctcagggac agcgagggca aattgcaggg gatacttgtt ttcctggtat cctcttgcgt
     5521 ccgttcatgc tagggtttcc cctatggaag tcatcataca accgaatcag gaagccgccg
     5581 cctcgctggt cgcgcggatc gtggcccgtg aacttcgcac caacccgcac ctcgtcctcg
     5641 ggttggcgac cggccagacg atggaatgcg tctatcgcca cctcgtccgc ctgcaccaga
     5701 ttgagaaact cgatttctca ctctgccgca cgttcaacct cgacgagtat gtcggtctgt
     5761 tccccgccga cccaaactca taccggcact tcatgaatca caacctgttc agccaggtga
     5821 acatagaccc gcggaacacc cacctgccca atggcctcgc gaccgacctg gacgaggaat
     5881 gccgccgcta cgaggccacc atcaagcggt gcgggggcat agacctgcag gtcctcggca
     5941 tcggcaagga cgggcacatc ggcttcaacg aaccgctctc cgctctccgg tcccgcaccc
     6001 gcgtcaaagc gctcacgcct accacactca agcagaacgc gcccttcttt ggcggggagg
     6061 acaagatgcc gcaccgcgcc atcactatgg gcgtcggaac tatccaggaa gcccgccggt
     6121 gcgttcttct cgccactggg gccagcaagg ctaatgtcat cgcccgggcc gtcgaggggc
     6181 ccattaccag catgatctcc gccagcgctc ttcaaatgca cccccgggcc accgtcattg
     6241 tggacgaaga agccgccggc aacctcatcg agaaggagta ctaccgctgg atcttcgcca
     6301 acgaacccga gtgggaagaa taccgctagt accgtgacag gttaaataag ttacatgagt
     6361 tacatcgtta caaacaccgg tggcgctggt cgctcatgta actg
//
LOCUS       DATCGS010000563         6970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_345466, whole genome shotgun sequence.
ACCESSION   DATCGS010000563 DATCGS010000000
VERSION     DATCGS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345466"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VIL39_11570"
     CDS             <1..758
                     /locus_tag="VIL39_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HEY5482113.1"
                     /translation="DHLRMQALRPGLQLRQAWAAIDQADSALEKKLDYAFSPELGYLT
                     ACPTNLGTGIRVSAMLHLPGLVLAEQINPIIQSVNKLGLAVRGLYGEGTEALGNVFQV
                     SNQMTLGESEGAIVERLEKVLAQIIEHEENARATLLEKKPKMVYNHIGRAYGILANAH
                     SISSKETMNLLSLMRVGVDMGSFPGVDRSLVDELFILTQPAHLQKQHSEKLSAEERDL
                     LRADMVRERLRPVSRPIVPPAVSPGAGLDKPTK"
     gene            791..3271
                     /locus_tag="VIL39_11575"
     CDS             791..3271
                     /locus_tag="VIL39_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY5482114.1"
                     /translation="MNNFTPRAQQVLALARKEADRFNHNFVGTEHLLLGLIKLGQGVA
                     VNVLQKLGLDLETVRLEVEKQVGTGPDQKMMGNIPYTPRVKKVLALAQKEAKALNHTY
                     VGTEHILLGLLREGDGVAARVLKNLDVDIEQTRQEILKELDPNFAAQDESAPGDAPGK
                     PATEKKGEIKTPALKAFGRDLTEIARKGEMDPVIGRKSEIERVIQILCRRTKNNPVLL
                     GEAGVGKTAIAEGLAQEIAAGNVPELLRDRRVITLDLALMVAGTKYRGQFEERIKAVM
                     DEIRRSKNIILFIDELHTIVGAGSAEGTMDASNIIKPALSRGELQCIGATTLNEYRKY
                     IEKDAALERRFQAVKVEAPSVDEAILILKGLRPKYEDHHKAEFTEKAIEASVRLSDRY
                     ITDRFLPDKAIDVMDEAGSRARIGTMTRPPEVKGLETEIEEIKAKKEKAIKNQDFEGA
                     AAMRDKEKQAKEKLETVLKEWRVSREEKRVKVDEEDILHVVSKWTGIPLQRMEQGEMQ
                     RLFQVETEMSKVVVGQREAVSSLCKALRRSRADLKDPRRPIGTFALLGPTGVGKTLLA
                     KTLAEQLFGDSKALIQLDMSEYMEKFNVSRLVGAPPGYVGYEEGGQLTEQVRRKPYSV
                     VLFDEIEKAHPDVWNMLLQILEEGKLTDNVGRIVNFRNTIILMTSNVGSDTIRKQSTL
                     GFSPITDENSYEKMREKIMDEAKKTFRPEFLNRLDDIIVFRSLTKPDLVQILDLEIAK
                     VMQRLKARNILLQLDEKAKDHLVSKGYDPLYGARPMRRAVERSLEDPLAEEILRGVFH
                     EGEPLLVTVDGDHLVFSQKTAAEGALSS"
     gene            3324..3512
                     /locus_tag="VIL39_11580"
     CDS             3324..3512
                     /locus_tag="VIL39_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482115.1"
                     /translation="MNFLKGSSDSYVARREIARKALKRTVFEENPHWADAPLGGLVDQ
                     GAVEQNDSGHYRIKRFEG"
     gene            3602..4771
                     /locus_tag="VIL39_11585"
     CDS             3602..4771
                     /locus_tag="VIL39_11585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY5482116.1"
                     /translation="MSLALGDKLTLTIDDIAFGGEGVARAEDFVVFVPFVAPGEVVEA
                     EVTEVKKRFARARLLRVVAASSDRVEPLCPHFGQCGGCQYQHLDYSLQLRLKHKQISD
                     LFQRIGHFAPGLIAPVVPCPQPYGYRNRIMIRSQWDKFKQGLNIGFLRADSRLVVDLE
                     ECKIAEPALNEQIKYVRAHPPPKGGIKVVLRIPPEGWEVPPDSFFQNNFFLLPRLVEA
                     AGDCLRRGGTRHLVDVFCGVGFFSLELGHLVESFVGVELDRLAIKAARRNAVARGRAN
                     GEFVAGAAEELLPALLSRFTAQATTVLLDPPRKGCRSETLQLLRRVRPAQIIYVSCHP
                     AAMARDLNALCAEDVFELAQVVPLDMFPHTQHVECVADIRCKLVAAGPKLSLPTL"
     gene            4774..>6970
                     /locus_tag="VIL39_11590"
     CDS             4774..>6970
                     /locus_tag="VIL39_11590"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY5482117.1"
                     /translation="MDSSLTRRMTKEREASSEKKFVPALLPWLVAAGGLVIYLVTLNC
                     WVSLSSLQQVAKVSGWTWQPELYGPLFWLVTYPFRWLPAQTIPLALNAFAAVCAALTL
                     ALLARSVVLLPHDRTQEQRQQELGKSGLLSIPTAWLPPLLAALVCGLQLTFWENATAV
                     SSPPAPWGSGCEVLDLLLFAYVIRCLLEFRLQERESWLLRAALVCSLGITNNWAMIGF
                     LPIFLVALVWIKGLGFFNLRFLTRMFVCGVAGLSLYLLLPLVQGVADTASVPFWSALK
                     TSLNAQKSILMLFYKYGRLEVALLALTSLVPVFIIGIRWASYFGDTSKSGQVLATSMF
                     HVVHGLFLVACIWVALDPPFSPRNKGFASPFVNYLTFYYLGALTVGYCSGYFLLLFRG
                     KPERGRRITPNMRLLNKAITSAIWLLLLLTPAALIYRNLAQIRITNGPMLRHFAALLA
                     QGLPSKRVVLLSEDSRRLLLLHAYAAQTGKGKEDLFLDTAALIYPDYHRFLKKKYPRV
                     WESNPPKGIVQSAEPFLLVQLISHLAQTNDIYYLHPSFGYYFEFFYPESHGLVHKLIP
                     YSTNLLFAPLPNRDLVAENETFWAKADKQALEPLLAAVAPPNSGKDHSLMEGLVKKAH
                     LTRESNHDAITLAAFYSRSLDYWGVEMQKSGQLTNAAAHFERALELNPDNLVAQVNLE
                     CNKELRAARKHSVHLSKSIEDEFGKYKKWEDILGENGPFDEPNFCYEQGR"
BASE COUNT         1463 a         1969 c         2046 g         1492 t
ORIGIN      
        1 aagatcatct gcgcatgcag gccctgcgcc ccgggctgca acttcggcag gcttgggcgg
       61 cgattgacca ggcggattcc gccttggaga agaagctcga ttatgccttt agtcccgaac
      121 tcggttattt gacggcctgt ccgacaaacc tgggcacggg cattcgggta agcgccatgc
      181 tgcacctgcc cgggctggtc ctggccgaac agatcaaccc cataattcag tcagtcaaca
      241 aactcggctt ggccgttcgc ggcctctatg gcgagggcac cgaggcgttg ggcaatgtct
      301 tccaggtgtc caaccaaatg accttgggtg aatctgaggg ggctattgtc gagcgactgg
      361 agaaggtcct ggcgcagatt atcgagcacg aggaaaacgc gcgggcaact cttttggaga
      421 agaagcccaa gatggtctat aaccacattg ggcgggcgta cggcatactg gccaatgccc
      481 acagcatctc ttcgaaagag accatgaatc tgctatcgtt gatgcgcgtg ggagtggata
      541 tgggatcgtt ccctggcgtg gaccggtccc tggtggatga gttgttcatt ctgacgcagc
      601 cagcccactt gcagaaacag cattcagaga agctttcggc cgaagaacga gacctgctcc
      661 gagcggatat ggtgcgcgag cgcttgcggc ccgtcagccg tcccatcgtt ccaccggcag
      721 tgtcgccggg tgctggattg gacaaaccga ccaagtaatt gcattttaaa gtatgagtga
      781 tcaggaagct atgaacaatt ttacgccgcg ggcgcagcag gtgctcgcgt tggcgcgcaa
      841 ggaagccgac cgtttcaacc acaactttgt cggcaccgaa cacctcttgc tcggtctcat
      901 caagttgggg cagggcgtcg cggttaatgt ccttcagaaa ctcggcctgg acctcgagac
      961 cgtccgtctg gaagtggaaa agcaggttgg cacgggaccc gatcagaaga tgatgggtaa
     1021 catcccttac acgccgcggg tgaagaaggt tctggcgctc gctcagaagg aggccaaagc
     1081 cctcaatcat acctacgtgg gcactgagca catcctgctg ggtctgctcc gtgagggcga
     1141 tggcgtggcc gcgcgcgtcc tcaagaacct cgatgtcgat atcgaacaga cccgtcagga
     1201 gatcctgaag gaactcgatc ccaatttcgc cgcacaggac gaatctgccc ccggcgacgc
     1261 cccgggaaaa ccggccaccg agaagaaggg cgagatcaag actcccgcgc tgaaggcctt
     1321 tggccgcgat ctgacagaaa tagcccgcaa aggcgagatg gaccctgtca ttggccgtaa
     1381 gagcgaaatc gagcgcgtca ttcagatctt gtgccgccgc accaagaaca atccagtgct
     1441 tttgggcgaa gctggcgttg gtaagacggc catagccgaa ggtttggcgc aggaaatcgc
     1501 ggcgggcaac gtcccggagt tgttgcgtga ccgccgcgtt ataacgctgg acctggcctt
     1561 aatggtcgcc ggtaccaagt atcgcggtca gtttgaagag cgcatcaaag ccgtcatgga
     1621 cgagattcgc cgctccaaga acatcatttt gttcattgac gagttgcaca cgatcgtcgg
     1681 cgccggctcc gccgaaggca cgatggatgc ctcgaacatc atcaaaccgg ctctcagccg
     1741 cggcgaactg cagtgcatcg gcgccaccac gctgaacgag tatcgcaagt acattgagaa
     1801 agacgcggct ttggagcgcc gcttccaggc cgtcaaagtc gaagctccgt ccgtggacga
     1861 agcaattctg attctcaagg gcttgcgtcc gaagtacgag gaccatcaca aagcggagtt
     1921 cactgagaag gctatcgaag cctcggttcg cctgtcagat cgttacatta ccgaccgctt
     1981 cctgccggac aaagccatcg atgtgatgga cgaagccggt tcgcgtgcgc gcattggcac
     2041 gatgacgcgt ccgccagagg tcaaaggttt ggagaccgaa attgaggaga tcaaggccaa
     2101 gaaggaaaag gcgatcaaga atcaggattt cgaaggcgcg gcggccatgc gcgacaagga
     2161 gaagcaggcg aaggaaaagc ttgaaacggt attaaaggag tggcgcgtca gtcgcgaaga
     2221 gaagcgcgtg aaggtggacg aggaagacat cctgcatgtg gtctccaaat ggaccggtat
     2281 ccccttgcaa cgcatggagc aaggtgagat gcagcgcttg ttccaggtcg aaaccgaaat
     2341 gagcaaggtg gttgtcggcc agcgcgaagc agtgtcctct ttgtgcaaag cgctgcggcg
     2401 ttcccgggcc gacctcaaag acccgcgccg acctatcggt acttttgcgt tgttgggccc
     2461 gactggcgtc ggcaagacct tgctcgccaa gacacttgcc gagcagttat ttggcgattc
     2521 caaggcattg attcagctcg acatgagtga gtacatggag aagttcaacg tctctcgctt
     2581 ggtcggggca ccgcccggtt atgtcgggta cgaggaaggt ggccagctta ccgaacaggt
     2641 gcgtcgcaag ccttactcgg tggtgctgtt cgatgaaatt gaaaaggcgc acccggacgt
     2701 ttggaatatg ctcttgcaga tcctcgaaga aggcaagctg acggacaatg tgggccgcat
     2761 cgtgaatttc cgcaacacga tcatcctcat gacctcgaat gttggctccg acaccatcag
     2821 gaagcaatcc accctgggct tctccccgat cactgatgaa aatagctacg agaagatgcg
     2881 cgagaagatt atggacgagg ccaagaagac cttccggcca gagttcttga atcgcctgga
     2941 cgacatcatc gtgttccgct cgttgaccaa gccggacctg gtccagatcc tcgacttgga
     3001 aatcgccaaa gtgatgcagc ggcttaaggc caggaacatt ctgcttcagc ttgatgaaaa
     3061 ggccaaggac cacttggtga gcaagggata cgatccactc tatggagcgc gcccgatgcg
     3121 ccgcgccgtc gagcgttccc ttgaggatcc actcgccgaa gaaatcctgc gcggggtctt
     3181 ccatgaaggc gagcccttgc tcgtgaccgt tgatggcgat catctggttt tctcacagaa
     3241 gaccgctgcg gaaggcgcgc tatcgagctg aatcacccgc acgatgctcc tggagactta
     3301 tgacatacga ggaacttgca attgtgaatt ttcttaaggg tagttcggac agttatgtcg
     3361 cgcgaaggga gattgctcga aaggccctga aacggacagt tttcgaggag aacccgcatt
     3421 gggcggacgc gccgctgggt gggctcgtgg accagggggc agtcgagcag aacgacagcg
     3481 gccattacag gatcaagagg ttcgagggat aattctctgg cagccgccaa gggacaaagg
     3541 cgacatcttc gttgcaagaa agtcctttta cctggctgac ggactttcta ctttctttgg
     3601 catgagcctc gctttgggcg acaagttgac cttgacgatt gacgatattg ccttcggtgg
     3661 cgaaggcgtc gcccgggcgg aggattttgt cgtcttcgtt ccctttgtcg ctccgggcga
     3721 ggtcgtggaa gctgaagtca cggaagtaaa gaagcgtttc gcccgagccc ggctgcttcg
     3781 tgtcgtggct gcctcctccg accgggtcga accactctgc ccgcacttcg gccagtgcgg
     3841 cggctgccag taccaacact tggactactc actccagctc cggctcaagc acaagcagat
     3901 tagcgatttg ttccagcgga ttggccactt cgcccccggt cttatcgcac cagtggttcc
     3961 ctgcccgcag ccttacggat accgcaaccg catcatgatt cgcagccagt gggacaagtt
     4021 taaacagggc ttgaacatcg gcttcctccg cgccgacagc cggctggtag tggatttgga
     4081 agaatgcaag attgccgagc ccgcactcaa cgaacagatc aagtatgttc gtgcgcatcc
     4141 gccccctaaa ggcggaatca aggtggtcct gcgcatcccg ccggagggct gggaagtgcc
     4201 gccggattct ttctttcaga acaatttctt cctgctgccc aggttggtgg aagccgcagg
     4261 tgattgctta cgccggggcg gcacgcgcca tctggtggat gtcttttgcg gtgtcggatt
     4321 cttcagcctg gaattgggcc acctggtcga gtcttttgtg ggcgtggaat tggaccggct
     4381 ggcgatcaaa gcggcgcgtc ggaatgctgt ggcccgcggg cgtgcgaacg gcgagttcgt
     4441 ggcgggagcc gccgaggaac tcttgccggc cttgctgtcg cgattcaccg cccaagcgac
     4501 gacggtgttg ctggatcccc cgcgcaaagg ttgccggtcc gagacgctgc aattgttgcg
     4561 ccgggtgcgg cccgcacaaa tcatttacgt ctcctgccac ccggcagcaa tggcgcgaga
     4621 tttgaacgct ttgtgcgccg aagatgtctt tgagttggcg caagtagtcc cgttggacat
     4681 gtttccgcat acccagcatg tggaatgcgt ggcggatata cgctgcaaac tggtggcggc
     4741 cggaccgaaa ctaagcttgc caactctgtg accatggatt catctttgac ccgccgcatg
     4801 acaaaggaac gcgaagctag ttcagagaag aagttcgtcc ccgcattact gccttggctg
     4861 gtagcggcag gcgggttggt tatctacttg gtaacgctca attgttgggt gtcgttgagc
     4921 agtctgcagc aggtagccaa ggtgtccggc tggacctggc aaccagagct gtacggtccc
     4981 ctgttttggc tagtcactta tccgttccgc tggctgccgg cccagacgat tcctctggcg
     5041 ctcaatgcct ttgcagccgt ttgcgcggcg ttgactctgg cattgttggc gcgctcggtg
     5101 gtattgctgc cgcacgaccg cacccaggag cagcgccagc aagagctagg caagtccggc
     5161 ctcctctcga tccccaccgc ctggctacct ccgcttctgg cagcgctggt ttgcggcctg
     5221 caactgactt tctgggaaaa cgccacggcg gtttcttctc cgccagcgcc ttggggctcc
     5281 gggtgcgagg tgctcgacct gctgctgttc gcttacgtga ttcgctgtct gctggagttt
     5341 cgtcttcagg aacgcgaaag ctggttgctg cgggcggcct tggtttgcag cttggggatc
     5401 acgaacaact gggccatgat tggtttcttg cccatctttc tcgtggcgct ggtttggatc
     5461 aaaggtctcg gtttcttcaa cctgcgattc ctcacgcgca tgtttgtgtg cggcgtggcc
     5521 ggtttgtcgc tttacctgct acttcccctt gtgcagggtg tggccgacac agcttcggta
     5581 cccttctggt ccgctctgaa gaccagcctg aatgcacaga agagcattct catgttgttt
     5641 tacaagtacg gcaggctgga ggttgccttg ctggccctga cttctttggt gccggtcttc
     5701 atcatcggta ttcgttgggc ttcatacttc ggcgacacca gcaaatcggg ccaggtgcta
     5761 gccacctcta tgttccatgt ggtgcacgga ctttttctcg tcgcctgcat ctgggtggca
     5821 ctcgaccctc cgtttagccc ccgcaacaaa ggcttcgcct ctcctttcgt gaactacctg
     5881 accttctact acctgggtgc gctcaccgtg ggctattgca gcgggtactt cttgctcttg
     5941 ttccgcggaa aacccgaacg cggccgccgc ataacaccca atatgcgcct gctcaacaag
     6001 gcgattacca gcgccatctg gctgctcttg ttgctcaccc cggcggcgct gatatacagg
     6061 aacctggccc agattcgcat caccaatggc cccatgctca ggcatttcgc cgcattgctg
     6121 gcacaaggtc ttccgtcaaa acgcgttgta ttactcagtg aagactcccg ccgattgttg
     6181 ctgctgcacg cctacgctgc ccaaactggc aaaggcaagg aggatctgtt cctggacact
     6241 gccgccctga tatatcccga ctatcacagg ttcctgaaga agaagtaccc gcgagtctgg
     6301 gaaagcaatc ctcccaaggg gatcgtgcaa agcgccgaac cattcctgct ggtccagttg
     6361 atttcgcatc tggcgcagac aaacgacatt tactacttgc atcccagctt cggctattat
     6421 ttcgagttct tctacccgga gtcgcatggt ctggttcaca agcttattcc ttattcgacc
     6481 aatctccttt ttgcgccgct gccgaaccga gacctggttg ccgagaacga gaccttttgg
     6541 gctaaggccg acaagcaagc gctcgagcca ctgctggcgg ccgtcgcgcc accgaattca
     6601 ggcaaggacc acagcctgat ggagggtctg gtaaagaaag cccatctcac cagggagtcc
     6661 aaccatgatg ccatcaccct ggcggctttt tattcccgct cactcgacta ttggggtgtc
     6721 gagatgcaga agagcggcca gttgacaaac gcggcagcgc acttcgagcg cgccttggag
     6781 ttgaatcccg acaatctcgt cgcgcaggtc aacctcgaat gcaacaagga gctgcgagcc
     6841 gcccgcaagc attcagtcca cttgtcgaag tccattgaag atgaattcgg caaatacaag
     6901 aaatgggagg acatcctcgg cgagaacggg ccattcgacg aaccgaattt ctgttacgaa
     6961 caaggccgcg
//
LOCUS       DATCGS010000564         4437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_393197, whole genome shotgun sequence.
ACCESSION   DATCGS010000564 DATCGS010000000
VERSION     DATCGS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393197"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VIL39_11595"
     CDS             complement(<1..1066)
                     /locus_tag="VIL39_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY5482118.1"
                     /translation="MLLAGCAVGPHYQRPPALGTNAMPASFAGATATNAGEWKSAQPS
                     AHLPRGSWWELFGDPELNRLEALATTNNQQLAAAFANFQQARALVNVARADFFPQLST
                     TPGVTRQRISANQSQRSIGSSTYNTFSVPLEASWELDLWGRVRREVEGARARLTASAD
                     DLESFKLAIQAEVAIDYFSLRSLDAQDQLLKETVVAYRRSLELTQNRRKGGIATELDV
                     SQAETQLASAEAQIPAVQLQRANLLHALAVLCGHAAPTFEVSVTQDALATARRASDAG
                     PGIPVAVPSELLERRPDIAAAERLMAAANADVGLAHAAFYPRVMLNGLAGFQSVDASS
                     LFNWESRVWSVGPSLSLPIFT"
     gene            complement(1292..>4437)
                     /locus_tag="VIL39_11600"
     CDS             complement(1292..>4437)
                     /locus_tag="VIL39_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5482119.1"
                     /translation="RSFAFGIRPSAVMNICAPFIRRPVGTSLLAIGLFLAGIIAYRFM
                     PVAAIPRVDFPMINVSASLPGADPATVASSLAAPLERRLAQIAGVTEITSVSTLGGCS
                     VTIQFDLNRNVDGAARDVQAAINAAAGDLPINLPNPPTYRKINPADAPVMILAMQSDI
                     LAPVQVFEYGDSIIGQRLSQVEGVSQAIISGADKSAVRVQVNPGALASTGLSLEDVRS
                     LLSQVNVDSPKGSFDGDQLSYTLTSNDQLLEGKGYQDLILTQRRGIPLRLSALGRAID
                     GTENARVAGWSGTNRAVLVIIFKQSDANVIETVDRIRAELPQLERWIPPSVKISEISD
                     RTTTIRVSVHDVQFSLVLSIALVVMVIFLFLRRFWPTFIASITVPLALAGTFAFMYLC
                     HYSIDNLSLMAVTISVGFVVDDAIVVIENIHRFIENGETPMQAAFKGARQISFTVISM
                     STSLVAVFIPLLFMGGLIGRLFHEFAVTVTLAILVSGVISLTLTPMLCSRFLRPESAY
                     RKPGLFLRACEQSFNWLLGHYETGLRWVLCHHNFMLVVAFLTLVATVVLYRAVPKGFF
                     PQQDTGIIMGTTDAAQDISFTAMKELQQKVAKIVLDDPAVATLGSFMGAGGGGSTVNN
                     GRMFITLKPKAERKVSADQVIARLRKKLGEVSGVNLFLQASQDIRVGGRMSRAQFQYS
                     LQSGDLNELNRWSTLLVDKLRLSPELRDVSSDQQTRGLQTLVVIDRDAAARLGVSPIA
                     IDNTLYDAFGQRQVSTMYKQYNQFHVILEVEPNYLLDPSALNQIYVRSTNGFQVPLAA
                     VAKFKPSNTFLSVNHQGQFPAVTLSFNLAPGVALGQATEVVERAVEELKMPSSVLGSF
                     QGTAQVFRASMSNMPLLIAAAILAVYVVLGMLYESLIHPLTIISTLPSAGVGALLALM
                     VFGLDLSLVSFIGIILLMGIVKKNAIMMINFALDAERQEGLSPRDAIYKACVIRFRPI
                     MMTTMAALFGSLPLAIGMGTGSELRQPLGIAVVGGLLLSQTLTLYTTPVIYLTFESLG
                     QRVKAWRRHRKLQFAPA"
BASE COUNT          883 a         1290 c         1521 g          743 t
ORIGIN      
        1 ccgtgaagat cggcagggat agcgacggcc ccaccgacca gacacggctt tcccagttga
       61 aaagcgagct ggcatccacg gactggaaac cagccagtcc gttaagcatg acgcgcgggt
      121 aaaacgcggc atgggccagg ccgacatccg cgttggcggc ggccatgagg cgctcggcgg
      181 cggcaatgtc aggccgtcgc tcgagcagct cactcgggac agccacgggg atgccgggac
      241 cggcatcgga ggccctcctc gcagtggcaa gcgcgtcctg agtgacgctg acctcgaagg
      301 tgggggcggc gtggccgcag aggacagcga gggcgtggag gaggttggcg cgctgcagtt
      361 gcaccgcggg aatctgggcc tcggcgctcg cgagctgagt ttcggcctgg gagacgtcga
      421 gttccgtggc gataccgccc ttgcggcggt tctgggtgag ttcaagcgaa cggcgatagg
      481 cgacaacggt ttccttcaac aactggtctt gagcatcgag cgagcgcaag ctgaagtagt
      541 caatggcgac ttcggcctgg atggcgagtt tgaaggattc gaggtcatcg gcggaggcgg
      601 tcaaacgggc ccgggcgcct tcgacctcac ggcgcacgcg gccccagagg tcaagctccc
      661 aactggcctc gagcgggacg ctgaaggtgt tgtaagtgga acttccgatg gagcgctggg
      721 actggtttgc gctgatgcgc tgacgggtga cgccgggcgt ggtcgagagc tggggaaaga
      781 aatcggcgcg ggcgacgttg acgagggcgc gggcctgctg gaagttcgca aacgcggcgg
      841 cgagctgctg gttgttcgtg gtcgcgaggg cttcgaggcg gttcaactcg gggtcgccga
      901 agagttccca ccacgagccg cggggcaggt gcgcggaggg ttgggcagac ttccattccc
      961 cggcgttggt ggcagtcgcg ccggcaaagg acgccggcat ggcgttagtg ccgagtgccg
     1021 ggggccgctg gtagtggggg cccacggcgc agccggccag gagcaagggt aaaatccgaa
     1081 ggccgaaggc cgaaacccga aagaaggccg aaacccgaag gccgaaaggg attagctggg
     1141 gccgcaggcc tacgctgggc acttcacagc cgacatggaa cactaacggg ccggagcgcc
     1201 ggcatgttgc cggcttggga ctcggacttc ggacttcggg cttcgggctt ctttcggcct
     1261 tcgggtttcg ggtttcggat ttcatatcat atcatgccgg agcaaattgg agtttacggt
     1321 gtcgtcgcca cgccttgacg cgctgcccca agctctcgaa cgtcagatag atcaccggcg
     1381 tagtgtaaag cgtcagggtt tgcgatagca gcaggccgcc gacgaccgcg atgcccaagg
     1441 gctggcggag ttccgagccg gtgcccatcc cgatggccag cggcagcgag ccgaacagcg
     1501 cggccatagt ggtcatcatg atgggtcgga agcggatgac gcaagccttg tagatcgcat
     1561 cgcgcggact cagcccctcc tggcgttcgg catcgagggc aaaattgatc atcatgatgg
     1621 cattcttctt cacgatcccc atcagcagaa tgatgccgat gaaggagacc agcgacaggt
     1681 ctaagccgaa gaccatgagc gcgagcaggg cgccgacgcc ggccgacggc agggtcgaga
     1741 tgatggtgag cgggtgaatg aggctttcgt agagcatgcc cagcacgaca tagacggcga
     1801 ggatggccgc tgcgatcagc agcggcatat tggacatgga agcgcggaag acctgggcgg
     1861 tgccctgaaa gctgcccaac acgctggacg gcatcttcaa ttcctcaacc gcccgctcga
     1921 cgacctcggt ggcctggccg agcgcgacgc ccggcgccag gttgaaggag agggtcacgg
     1981 caggaaactg tccctggtga ttgaccgaca ggaaggtatt cgacggtttg aacttggcga
     2041 cggcggccag cggcacctgg aacccgttgg tggaccggac gtagatctgg ttgagcgcag
     2101 aggggtcgag caggtaattg ggttccactt cgaggatgac gtggaactgg ttgtattgct
     2161 tatacatggt cgagacctgc cgctgaccga acgcgtcgta gagggtattg tcaatggcga
     2221 tgggggagac gcccaggcgt gccgccgcgt cacggtcaat gaccaccagg gtttgcaggc
     2281 cgcgcgtctg ctggtcgctg ctgacatcgc gcagttccgg cgagaggcgc agcttgtcca
     2341 ccaggagcgt ggaccagcgg ttgagttcat tgaggtcgcc cgactgcaac gaatattgaa
     2401 actgcgcccg gctcatccgc ccgccaacgc ggatgtcttg cgaggcctgg agaaacaaat
     2461 taacaccgct gacctcgccc agcttcttgc gcaagcgcgc gatgacctgg tccgcgctca
     2521 ctttccgttc cgccttcggt ttcagggtaa tgaacatgcg gccgttgttg accgtcgaac
     2581 cgcccccgcc cgcgcccatg aaagaaccca gcgtggccac cgccgggtcg tcgaggacga
     2641 ttttcgccac tttctgctgc aactccttca tggcggtgaa ggaaatatcc tgggcggcgt
     2701 cggtcgtgcc cataatgatg ccagtgtcct gctgggggaa gaagcccttg ggcacagccc
     2761 ggtaaaggac cacggtggcc accagggtca ggaaggccac gaccagcatg aagttgtgat
     2821 ggcacagcac ccatcgcagt cccgtttcgt aatggcccag cagccagttg aacgactgct
     2881 cacaggcgcg aaggaagagt ccgggcttcc gataagccga ttccggtctc agaaaacggg
     2941 agcagagcat cggcgtgagc gtcagcgaaa tgacgcccga aaccagaatg gccaaggtta
     3001 cggtcacggc gaattcgtgg aacagccgcc cgatcagccc gcccatgaac agcagcggaa
     3061 tgaacaccgc caccagcgac gtgctcatcg agatcaccgt gaagctgatc tgccgcgccc
     3121 ctttgaaggc cgcctgcatc ggcgtctcgc cattttcaat gaagcggtgg atgttctcga
     3181 tcaccacaat ggcgtcatcc accacgaacc cgacggagat ggtgacggcc atcagggaga
     3241 ggttatctat gctgtagtgg cacaagtaca tgaaggcgaa ggtgccggcc agcgccagcg
     3301 gcacggtgat gctggcgatg aacgtcggcc agaaccgccg caggaacagg aagatgacca
     3361 tcaccaccag cgcgatgctc agcaccagcg agaattgcac gtcgtgcacc gagacgcgga
     3421 tcgtggtcgt gcggtcgctg atctcgctga tcttcacgga ggggggaatc caccgttcga
     3481 gctggggcag ctcggcgcga atgcggtcca cggtctcgat gacattggca tccgactgct
     3541 tgaagataat gaccagcacg gcgcgattgg tgccggacca ccccgccacc cgcgcatttt
     3601 cggtgccgtc tatcgcccgg cccagggcgc tcaatctaag agggatgccg cgccgctggg
     3661 tcagaatcag gtcctggtaa cctttgccct ccagcaattg atcgttgctg gtcagcgtgt
     3721 aggaaagctg gtcgccgtca aaactgcctt tgggcgaatc cacgttcacc tggctgagca
     3781 gggaacgcac gtcttccagg ctcagccccg tcgaggccag cgccccgggg ttgacctgga
     3841 cgcgcacggc tgatttatcg gccccgctga taatcgcctg gctgacgcct tcgacctggc
     3901 tcaaccgctg cccgatgatg gagtcgccgt actcaaaaac ctgcacgggg gcaaggatgt
     3961 ccgactgcat cgccaggatc atgacagggg cgtcggccgg attgatttta cgataagtgg
     4021 gcgggttcgg caggttgatg ggcagatcgc cggcggcggc attgatggcc gcctgcacgt
     4081 cgcgcgccgc gccgtccacg ttgcggttca ggtcgaactg gatggtgacc gagcagccgc
     4141 ccagggtgct gacggaggtg atttcggtga cgccggcaat ctgggccagg cggcgttcca
     4201 gcggcgcggc cagggaggag gccaccgttg ccgggtcggc gccgggcagc gaggccgaca
     4261 cgttgatcat cgggaagtcc acgcgcggaa tggccgccac cggcatgaag cgataggcga
     4321 tgatgcccgc caggaataaa ccgatggcca gcagcgacgt gccgaccggc cggcggatga
     4381 atggggcgca aatgttcatg acggcggaag gccgaatccc gaaagcgaaa gaccgaa
//
LOCUS       DATCGS010000565         3414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_494347, whole genome shotgun sequence.
ACCESSION   DATCGS010000565 DATCGS010000000
VERSION     DATCGS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494347"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1688)
                     /locus_tag="VIL39_11605"
                     /pseudo
     CDS             complement(280..1688)
                     /locus_tag="VIL39_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1895..2917
                     /locus_tag="VIL39_11610"
     CDS             1895..2917
                     /locus_tag="VIL39_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028714619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5482120.1"
                     /translation="MTQYKRIGIDTSKAVFTLHCVDQQDRPVLRINLRRAQMNPFFAK
                     LPPTEIAMEACGGAHHWARELAALGHEVRLIPPQYVKPYVKRGKNDRNDAEAICEAAG
                     RPGMHFVPIKSVSQQAQGMVLKVRETLVGQRTLLVNTLRGHAAEFGMIAGKGLPQVGP
                     LLTAIERETTIPPEARDMAALLGRQIEDLDTRIKELDAKLTAVHKANAVSQRLATIPG
                     VGPVTALTLAIEIDPAAFESGRHLAAWAGLTPKEQSTGGKQRLGGISRAGNERLRVLL
                     VAGATSVINAAMRPGSKQMTDWLRALLQRKPRKVAAVALANKMARVAWALMARGEVYR
                     RPAAAA"
BASE COUNT          593 a         1025 c         1180 g          616 t
ORIGIN      
        1 ggaatgttcc attctcctcc atgagcagaa ggtggtagcc acgatacggc tctttgccaa
       61 ttatcatctc gtcctcctcc ctcatgcatt atgcctaggc tcgattgtgt gacctagcca
      121 aacatttccc agatgggcgc aaaaccggcg ctggtggcga aatgttaccc ccggaccaga
      181 gcgccccgca atcgagatat gattgggaac gacactggta gcctcccgga caacgcgctc
      241 ggaacgtgtc aacgacaatg agacggccgg tcttcagaat taggcttgga ctgcgagctt
      301 ctggtttggc ggattgatcc agacggcggt gggttttgtc ggcggttcgg ggggcttgtt
      361 gacgaagcgg ttcgggttgg cgtggaaggc ctggttgagg attctctggc gggcggcgtg
      421 gacctcatca gtctgtccgt agtggacttg gtctggggtc atcaggccga tgccgaggtg
      481 gtgatggtcc tggttgtacc agtcgaagaa ggcccggcag aaggtcttgg cgtcctggat
      541 gcagccgaac cgcttgggga actggggctg gtatttcagg gtcttgaagc agctctccga
      601 gaaggggttg tcgttggagg tatagggccg gctgtgtgat ttggtgatgc ccaggtcggc
      661 gagcagcagg gcggtggcct tggccttcat ggaggggccg cgatcggcgt gcagggtgag
      721 ttggccgggc gggacgtcgt gcttggcggc ggcgtcctcg aacaggggct tgaagagagc
      781 ggcgctctcg gtgtcggcga cgcaccagcc gacgaccctg cggctgaaga tgtcgatgat
      841 cacatagaga tagaagtagg tccatttggc gggccccatc agcttgctga tgtcccagga
      901 ccacacctgg tttgggccct cggcgatgag ttcgggcttg gcgtagacgg ggtggcgcag
      961 ctggcggcgg cgctctttca cgtcgccatg ctcatggagg atccggtaca tggtgcggat
     1021 cgagcaatgg tagaggccct cgtcgagcag gctggcgtag acctccgccg gggcctgatc
     1081 gacgaaccgc ggctcgcgca gcagatcgat gacgccttgg cgttcgtccg ccgccagcgc
     1141 ccgcgcgggc ttgggtcggg gacggacagt cgcgggcggt cgtgtcgccg cggcccggcg
     1201 gcgatggaag ctggcgcggg gcagccccag ggcggcgcag gcggcggcga tcacgcctgg
     1261 ctcgggcgcc agagtggcca cggcgtccat caggtctcct cgtcgctgac gatcggcagg
     1321 cccagaagca aggccacttt tttttggatg tcgatgaccg cctcggcgcg ccgcagctgg
     1381 agcgtgaggc gtttgttgtc gcgcagcaat tgggcgtgct cggcggccag gggattggcc
     1441 ggcgcgcgct tggggccgcg cttgacggcc ttcagggcct cataggcgcc ggcgtcacgt
     1501 tggcggcgcc agtcggtcag ggccgacgaa tagagcccct cgcgccgcag gatcgcgccg
     1561 gcgcccccag gggcggcccg gtcaacctcg ccgagaatgc gaagcttgtc ggcggccgtg
     1621 aacgtgcgcc gacgcggacg gtccacaagc tcgggcgacg ccgccggtgg cgctgaaacc
     1681 agcgtcggcc tacggccttc ctcgttttca gcgccaccgg cggcaaaggg ctctttggct
     1741 gtcgtgcgag gcatgaccat ttcgatgttt cacctgacgc cctcaagggt aaacttccgg
     1801 ggggtcgatg tctcatcgtc attgttgggg tggacggccc cacggcatcg cgtgccagaa
     1861 ttggggctgt gagcaaccaa gaccggagcc gtccatgacc cagtataaac gcataggcat
     1921 cgacacgtcc aaggccgtct tcaccttaca ctgtgtcgat cagcaagatc ggccggtgtt
     1981 acggatcaat ctccgccgcg cgcaaatgaa ccccttcttc gcgaagctcc cgcccaccga
     2041 gatcgccatg gaagcctgtg gcggcgctca ccattgggcg cgcgaactgg ccgcgctcgg
     2101 tcacgaggtg cgtctcatcc cgccccaata cgtcaaaccc tacgtcaaac gtggcaagaa
     2161 tgatcgcaat gacgccgagg cgatctgtga agcagcgggg cgacccggca tgcatttcgt
     2221 gccgataaag tctgtttcgc aacaggctca gggcatggtt ttgaaggtgc gcgagaccct
     2281 ggttggtcag cggaccctgc tggtcaacac gctacgtggc cacgccgccg aattcggcat
     2341 gatcgccggc aaggggcttc cccaggttgg cccgttgctg actgccattg aacgggagac
     2401 caccatcccg cccgaggcca gggacatggc cgccctgctg ggccggcaga tcgaggatct
     2461 cgacacaagg atcaaggagc tcgacgccaa actgaccgct gtgcacaaag ccaacgcagt
     2521 cagccagcgc ctggccacga tccccggcgt aggcccggtc accgcgctga cgctggcgat
     2581 cgagattgat ccggcggcgt ttgaatcggg tcgtcacctg gccgcctggg ccggtctgac
     2641 accaaaggaa cagtcgaccg gcgggaagca gcggctgggt gggatcagcc gggcgggcaa
     2701 tgaacggttg cgcgtcctgc tcgtcgctgg cgccacctcg gtgatcaatg ccgccatgag
     2761 gccgggcagc aagcaaatga cggactggtt gcgggctcta ctgcaacgca agccgcgcaa
     2821 agtcgcggcc gtggcactcg ccaacaagat ggctcgggtg gcatgggcgc tgatggcgcg
     2881 cggggaagtg taccggcgcc cggcggccgc ggcatgaccg gcatttcgtc acgtaacgcc
     2941 atggagtgaa ctcatgcccc gttcattggt cggtgatatc cccatgaccg gcgccgaacg
     3001 tcaggcgcgc taccgcgccg cgcggtttcg gccgtgattg aacaacggga tggcccagac
     3061 ggcatgggtc gcgatgacgc gcagggcggc ttgccggcat cagccggtga ccgcctgaac
     3121 cgcgcgtcga tgccggggtg aaagagctga atgagatgac gatcggtcga accgacgatc
     3181 cagagaaccc gttctgtgcc acggctttca aagccgcctc attgtttggg actggatcgc
     3241 ggaccccatc tgggccagcg gtcatggcgc cgcgtcaaca ggccggacac atgatcgcaa
     3301 tcgaaccgcc cgtcagattt ctcattttcc tcttgcatcc gaggggccgt ccacacatgg
     3361 cacggagggc ggtgtggcgg gagattgagt cgctccaccc tgccagtgca ccag
//
LOCUS       DATCGS010000566         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_243373, whole genome shotgun sequence.
ACCESSION   DATCGS010000566 DATCGS010000000
VERSION     DATCGS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243373"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..766
                     /locus_tag="VIL39_11615"
                     /pseudo
     CDS             1..766
                     /locus_tag="VIL39_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            complement(807..1139)
                     /locus_tag="VIL39_11620"
     CDS             complement(807..1139)
                     /locus_tag="VIL39_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HEY5482121.1"
                     /translation="MIEDTIGKIEARIQGAEAVKEERRQELLQLLGTLKSEVAELSKT
                     HGEQAQSIAGFAELSTHEATRTEPNPELLKLSLEGLGSSVQGFEGSHPRLVQIVNAIS
                     STLSNLGI"
     gene            complement(1256..1912)
                     /gene="tmk"
                     /locus_tag="VIL39_11625"
     CDS             complement(1256..1912)
                     /gene="tmk"
                     /locus_tag="VIL39_11625"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5482122.1"
                     /translation="MKVPFITFEGTEGSGKTTQISLLADRLRSMGHIVRTLREPGGTP
                     IGEEIRHTLKHSPANHAMTSEAELLLMNASRAQLVREVIRPALAAGEIVLCDRFYDST
                     TAYQGYGRQLDLQMVKSIIDVAVGETRPDLTLLFVVPQEVSEARRLARQPTLPLPFKR
                     DRIEEAGQNFFARVAEGYQAIAAAEPQRVRQFDATGDVARIQAAIWDLVQPLVKKLKR
                     "
     gene            complement(1914..>2500)
                     /locus_tag="VIL39_11630"
     CDS             complement(1914..>2500)
                     /locus_tag="VIL39_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEY5482123.1"
                     /translation="GKKMSKSVGNFFTLRDLLAKGFTGREIRYLLLTAHYRETFNFTL
                     EGLQGARASLTRIDECLTKLRELASQTKATADSPLLSEFSAALDDDLNISGAWGAIFE
                     WVRDTNRKLAENIIDPAAAAVALATWEKLDSVLGVGAPKEVEVPVEITALVEARQAAR
                     KAKDFKRADALRDELKAKGWGIADTPKGARAKRL"
BASE COUNT          460 a          773 c          763 g          504 t
ORIGIN      
        1 caacatcaac accgagatga attattggcc ggccgagccg tgcaatctgg cggagtgcgt
       61 cgagccgctg attgcgatgg tgaaggacct cagcgaaacc ggggcccgca ccgcgaagac
      121 catgtatggc gcgcgcggtt gggttaccca tcataatacc gacctgtggc gcgccacggc
      181 gccgatcgac ggcccggcct gggggatgtg gccgagcggc ggcgcctggc tctgcctcca
      241 actgtgggat cattaccagt tcagcggcga taagaagtac ctggccggcg tttacccggt
      301 gctgaaaggc gcagcccagt tcttcctcga cacgctggtg gaggaaccca agcacaaatg
      361 gctcgtcacc aatccttcga tatcgtctga gaacgagcat ccattcggtt catcggtgtg
      421 cgccgggccg acgatggacg agcagatcat tcgcgacctg ttctcgaaca cgattcaggc
      481 tgcggagctg cttggcctag acaaggggct gcgacagcaa ttggcggcgg tccgggcgcg
      541 gctggcgccg aaccaggtgg gcaaggccgg ccagttgcag gagtggctgg aggattggga
      601 tgcgcaagct tccgaccaga agcattggca tatctcccac ctctatgggc tttacccgag
      661 cgcgcaaatc actccccgcc agacgcccga gctcgccgcc gccgcaaaag cctcactgaa
      721 tacgcgcggc gacatcacga cgggctgggc gattgcgtgg cgcatcacca acagcagcgg
      781 tcttcgcccc cattccttcg ccctggtcag atgcccagat tcgacaacgt gctgctgatg
      841 gcgttgacga tttgaacgag gcgcgggtga gacccttcga atccctgcac ggaggagccc
      901 aagccttcga gtgagagttt gagcagttcc gggttcggct cggtgcgagt ggcttcgtgg
      961 gtagagagtt ccgcgaaccc ggcgatgctt tgggcttgct ccccgtgcgt cttggacaat
     1021 tcagcgactt ccgatttcag cgtgccgagc aactgcagga gttcctgccg acgttcctct
     1081 ttgacagcct cagcgccttg aattcgggct tcgatctttc ctatggtgtc ctcaatcatc
     1141 gctttgcttt cgattccatg ctcaggatag tcctccttga ccggtcagca agcaaaacaa
     1201 gcgacagcaa aacaagcgat ttgccgcggg tgtatctttc attcaggtaa tagggttaac
     1261 gcttcagctt cttaacgagc ggctgcacga ggtcccaaat ggccgcttga atcctggcga
     1321 cgtctcccgt cgcgtcaaac tgccgcaccc gttgcggctc ggcggcggcg atcgcctggt
     1381 agccttcggc gacgcgcgca aagaaattct ggcccgcctc ctcaatccgg tcccgcttga
     1441 aaggaagagg cagggtgggt tggcgggcca gccggcgggc ctcgctgacc tcctggggaa
     1501 cgacaaaaag caaagtcaaa tccggccggg tctcgccgac ggccacatca atgatgctct
     1561 tgaccatctg caaatccagt tgccggccat agccctggta tgccgtggtg gaatcataga
     1621 agcggtcgca cagaacaatc tccccggcgg cgagtgccgg ccgtatgacc tcccgaacca
     1681 attgagcgcg gctggcgttc atcaggagca gctcggcttc ggaggtcatc gcgtgattgg
     1741 ccgggctgtg cttgagcgtg tggcggattt cctccccgat gggagtgccg cccggctcgc
     1801 gcagggtgcg gacgatgtgg cccattgacc gcagacggtc tgccagcaaa gaaatctgcg
     1861 tcgtcttccc gctgccttcc gtgccctcaa aagtgatgaa tggaaccttc atgtcacaag
     1921 cgtttggctc gcgccccttt gggcgtgtcc gcgatgcccc agcctttggc cttcagctcg
     1981 tcgcgcagag cgtcggcgcg tttgaaatct ttggctttgc gcgcggcctg ccgggcttcg
     2041 accagcgcgg tgatctccac cggcacttcc acttccttgg gcgcaccgac acccagcacc
     2101 gaatccagct tttcccaggt ggccagcgcg accgcagcag cagcggggtc tatgatattc
     2161 tccgccagct tccggttagt gtcgcggacc cattcaaaga tggcgcccca ggcgccggag
     2221 atattcaggt cgtcatccag cgcggcggag aattcgctga gcaacggtga gtcggcggtt
     2281 gctttcgtct ggctcgccag ctcgcgcagc ttcgtcaggc attcgtctat ccgggtcagt
     2341 gaagcccgcg ctccctgcaa accttccagc gtgaaattga acgtctcccg atagtgggcc
     2401 gtcagcagca ggtagcggat ttcgcgcccg gtgaatcctt tcgccagcag gtcgcggagg
     2461 gtaaagaagt tcccgacgga cttgctcatc ttcttgcctt
//
LOCUS       DATCGS010000567         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_295177, whole genome shotgun sequence.
ACCESSION   DATCGS010000567 DATCGS010000000
VERSION     DATCGS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295177"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VIL39_11635"
     CDS             <1..1238
                     /locus_tag="VIL39_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482124.1"
                     /translation="QDTNGWAQLPLYQPAGVLALEIPRADGKTGVLQVDTGNPEGVSL
                     SPARWKEWRATHPHAHGGWQFNFMPGSGPAIGRQYEADDLAIGPLTWKRVTLRKARRT
                     ETNIADGGDVFEASIGIAALRQLNLIIDRTNHMAYVRPNPDWVPSKEAHRKKTSCRAA
                     ASTNSTVRLGFKEHEYGDAAMESFESGKFDAAITNATCLLALQPTNAGAFALRGIALF
                     RLHSADGSATNLDKAQGDLSRALELDPGIIAAYSIRGHIYYLTQRWDDGVRDFRRFCD
                     KAPDEANYPRFFIWLMRARTGEPAAADQELSAWFGPGKKLKGDRWQGNIGAFLLGRMT
                     EADFLGAGNRGHDSGRQCEAWFYAGMKRLLSGEAATARDDFQKCLATDRKDYDEYSFA
                     AAELRMLESQMRGQPRLGF"
     gene            complement(1796..>2701)
                     /locus_tag="VIL39_11640"
     CDS             complement(1796..>2701)
                     /locus_tag="VIL39_11640"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5482125.1"
                     /translation="CDTLPAMLGLEQLAELLETNRAAAKARVKEFSIGGKHFAFNSQP
                     AIMGVINLSADSWYRESVCVTAESAVRRAKVMREQGADIIDVGAESTLAHAARVDEAE
                     QNSKLLPVIRALRESGILVSAETYQPAVTRACLEAGANILNLTGTDQTDELFRMVAAH
                     DAAVIICYVQGRNVREVGDFNFSADLIALMQDYFARQVELAVRNGVEKILIDPGLGFY
                     YRNLQDSSVRVRHQMTVFLNTFRLRALGFPICHALPHAFEYFGEEVRCAEPFFAVLAA
                     LGKTDLFRTHEVPRIKAVLDTLRVF"
BASE COUNT          548 a          835 c          822 g          496 t
ORIGIN      
        1 cgcaggatac gaacggatgg gcccagctcc cgctctatca gcccgcgggc gtgctggctc
       61 tggagattcc ccgtgccgat gggaaaaccg gtgtcctgca agtggatacg ggcaatccgg
      121 aaggcgtcag tctttcgccc gcgcgctgga aggaatggcg cgcgacgcat ccccacgcgc
      181 acggggggtg gcaattcaac ttcatgcccg gttccggccc ggccataggg cggcaatacg
      241 aggccgacga tttggccatc ggcccgctga cgtggaagcg tgtgaccctt cgcaaggcgc
      301 gccggactga gacgaacatc gctgacggcg gcgacgtttt tgaagcaagc atcggcatcg
      361 ccgcgctgcg tcaattgaac ctcatcattg accggacaaa ccacatggcc tacgtgcggc
      421 ccaacccgga ttgggttccg agtaaggaag cccatcgcaa gaaaacctcg tgccgggcgg
      481 cggcgtccac caatagcacc gtgcggcttg gtttcaagga gcatgagtat ggggacgccg
      541 caatggagtc cttcgagtcg ggcaagttcg atgccgccat cactaatgca acctgcctgc
      601 tagcgctgca acccaccaac gcaggagcct tcgctcttcg cggcatagcc ctattcaggt
      661 tgcactccgc agatggcagc gcgaccaacc tggataaggc gcagggcgac ctcagtcgcg
      721 cgttggaact ggacccggga atcatcgccg cttacagtat tcgcggccac atctactacc
      781 tcacgcagcg ctgggatgat ggcgtgaggg atttccgtcg cttctgcgac aaggcccccg
      841 atgaggcaaa ctacccgcgg tttttcatct ggctgatgcg cgcccgaacg ggcgaaccgg
      901 cggcggcgga ccaggagctg agcgcctggt tcgggccggg gaagaaactg aagggagacc
      961 gctggcaggg aaacatcggc gcgttcctgc tgggccgcat gaccgaggcc gattttcttg
     1021 gcgcaggcaa tcgcggccac gattccggac ggcaatgcga ggcgtggttc tacgccggca
     1081 tgaagcgcct cctctcgggc gaggccgcca cggcgcgtga cgatttccag aaatgcctgg
     1141 cgacagacag gaaggactat gacgaataca gcttcgccgc cgccgagttg agaatgttgg
     1201 aatcgcaaat gcggggtcaa ccccgtctcg gattctgagg tcggctcctt taccctggac
     1261 catccgcaat acgggggctg gggcgcgacc atcgtttgcc tgcgcaaacc tcaggcgtaa
     1321 cagcgcgttg actctgcctc caagtgaact tgcattgctg gtccattgct gcaccaggcc
     1381 agcctgattc tcgaaaacaa ccgagccctg cgcaaatgcc atcgtggggt gacgatcaaa
     1441 acgacgagtg tgagtgcctt attctcgagc acctgcgccg gcgagccttc ctgcctgcga
     1501 gtccggcttg acgcggttcg tgagttgaga ttgcatcagg cgacggtctg agcacgaaac
     1561 acagctatga gtatgaatgt tatccccggt gaggctccca tgtgggtccg gtgactctcc
     1621 catgacgtct cttcggagtc aggcagtgtg aaagaggcac tttatgaaaa cgtataagaa
     1681 gcccgaatcc atccgtcccg ttctccccgc ctcggggtgc cctcccactg gtctggtacc
     1741 accctggact gacccttgta cgaaaactgg gcgcagcaaa agtctgttgt tagggctaga
     1801 agacccggag cgtatcgaga accgccttga tgcggggcac ttcatgcgtg cggaacaaat
     1861 cggtcttgcc cagcgcggcc agcacggcga agaagggttc cgcacagcgc acttcctcgc
     1921 cgaaatactc gaaggcatgc ggcagggcgt ggcagatcgg gaagcccagc gcgcgcagcc
     1981 ggaacgtgtt caagaaaacc gtcatctgat gccgcacgcg cacgctgctg tcttgcaggt
     2041 tgcggtagta aaagcccagg ccaggatcaa tgagaatctt ctccacgccg ttacggaccg
     2101 cgagctccac ctggcgcgca aagtaatcct gcatcagggc gatcagatcg gcgctgaaat
     2161 tgaaatctcc cacctcccgc acattccggc cctggacgta acaaatgatc accgcggcgt
     2221 cgtgcgccgc gaccatgcgg aagagttcat cggtctggtc ggtgccggtg agattcagga
     2281 tgttcgctcc ggcctcaaga caagcgcgcg tcacggccgg ttgatatgtc tccgccgaaa
     2341 ccaaaatgcc cgattcccgc agcgcccgga tgaccggcag taacttgctg ttctgttccg
     2401 cctcgtccac gcgcgcggca tgggccaaag tggactccgc gccgacgtcg atgatgtccg
     2461 ccccctgttc ccgcatcacc ttggcccggc gcaccgcgct ctctgctgtg acgcagacac
     2521 tctcgcggta ccaggaatcc gccgaaagat tgatcacgcc catgatcgcg ggctgcgagt
     2581 tgaaggcaaa gtgcttgccg ccgatggaga attccttaac gcgtgccttg gcggcggctc
     2641 gattcgtttc gagcaactca gccagttgtt ccagtcccaa catggcaggc aaggtatcgc
     2701 a
//
LOCUS       DATCGS010000568        10294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_295286, whole genome shotgun sequence.
ACCESSION   DATCGS010000568 DATCGS010000000
VERSION     DATCGS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295286"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VIL39_11645"
     CDS             complement(<1..614)
                     /locus_tag="VIL39_11645"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY5482126.1"
                     /translation="MKKSLFARWQANFWAGLAIVLPAVISIAVLRWLFGTVANITDTL
                     LIFLPTKLTHHDAGNGPMYWYWSVVALALAIFLIGTVGLLARNYFGRKIIEWVDSALL
                     RIPLLNKIYGATKQVNDAFSSSNKTAFRTVVLVEFPHPAVYSIGFITSDQQEEVEAKT
                     GQKVVCVFVPATPNPTSGFLLMVPEGKVIKLEMSVPDAIKYIISL"
     gene            complement(687..1316)
                     /gene="ybeY"
                     /locus_tag="VIL39_11650"
     CDS             complement(687..1316)
                     /gene="ybeY"
                     /locus_tag="VIL39_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY5482127.1"
                     /translation="MATGLPYCKGERHERAIRGHDAGPDRQRILYLRNQQAVHSVHLG
                     LLRRIARALLEETWRDGSFDLAIYLVAASEMTRLNEAFLRHKGSTDVITFDYAERAGQ
                     GARPSRFPAPSGRFRGRRDVRPALLHGEIVACLDEAVCQARRFHTTWQSELVRYVVHG
                     VLHLLGYDDQDNRARRRMKAAEDALVRRLARRFVLSALSRTWKGRAADV"
     gene            complement(1339..2322)
                     /locus_tag="VIL39_11655"
     CDS             complement(1339..2322)
                     /locus_tag="VIL39_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY5482128.1"
                     /translation="MPTETLHFENARVAQQLYNNDPRNLQAVEHQLGVKATSREGWIK
                     LDGESDAVDRAKQLFLLLEHSLKAGTPVRNREFTHALNIVKHEGVPMLKDILSDRIQT
                     SEKKPSVTAKTVGQKKYLDAIRRHDVTIGVGPAGTGKTYLAVAMALAALREGQVSRII
                     LTRPAVEAGEALGFLPGDLYEKIMPYLRPLHDALHDMLPAEEVQKQTERGVIEIAPLA
                     YMRGRTLNNAFIILDEAQNSTTEQMFMFLTRLGISSKAVITGDETQIDLPVHKQSGLL
                     EAHRALKFTEGIAIVEFSKRDVVRHPLVQRIIAAYEEHRSQSRPRPAANAE"
     gene            complement(2389..2877)
                     /locus_tag="VIL39_11660"
     CDS             complement(2389..2877)
                     /locus_tag="VIL39_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5482129.1"
                     /translation="MESLHAPWRIEYILAPKPALDESVFTRIAQSSDDEANHVVARDR
                     TCFALLNTYPYTGGHLMVVPYKQVPDLNGLTDEELADLLKLTRRCQNALSRVMKPSGF
                     NIGMNLGQVAGAGVLGHLHIHIVPRWQGDTNFMPVLANTVVVPEALRDLAAKLRTALA
                     ED"
     gene            complement(3066..5561)
                     /locus_tag="VIL39_11665"
     CDS             complement(3066..5561)
                     /locus_tag="VIL39_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5482130.1"
                     /translation="MNLPASQVLLVEDDPRMPEVLSGLLQDDNITLSSASDAATGLKL
                     AREKQFDVILLDLGLPGVNGMELLRQLKESPETQTIPVIVLTAWNSTTDKLRGFELGA
                     VDYLTKPFEPAELRARLRSVLRTKHLRDELIQANRELLAARMAAEAATRAKAEFLANM
                     SHEIRTPMNGIIAMSGLLLETTLSHEQHGYVETVYSSSESLLTIINDILDFSKIESGK
                     LELESQPFDLRACIEDGLDLLAPKAAEKKLDVAYQMDDGIPAQMVGDVTRLRQVLVNL
                     LSNAIKFTSAGEVVVQVKVLSAPEQTASGAEPWQLHFLVRDIGIGIPVDQLARLFKSF
                     SQADASTTRQYGGTGLGLAISKRLVEMMGGRMWVESVPRKGSTFQFTLPLQAAPDAPL
                     HALEGRLPQLADLRLLIVDDNPTNCRILALQTRKWGMVPRDTRDAAQALEWLRAGESF
                     DLAILDMQMPNMDGLMLASEIRKLPGRGTLPLVLLTSIGVHSDHPDFARASIAGCLTK
                     PIKPAQLRETLLRALSGSKPAVLKAVAPSAKHDPTCASRFPLRMLLCDDNIVNQKVAL
                     RLLQQMGYRADLAANGLEVLEALDRQPYDLVFMDVMMPEMGGLEAARLIRERQQQPSQ
                     FPNYKSSIIVVAMTASAMQGDREKCLAAGMDDYIAKPVRLEDVRRIVERWAAVAGRTK
                     PAETTATGTQSPPGANAPAQSSPAAAPPEDEPVDMARLLDFTNGDQNNLRELATLYLD
                     QTGQQVEQLEAAVQAGAAQEVRRLAHSCAGASATCGMRRMVPLLRELERQGFEERLTS
                     AAELSHQVGEEFKRIRQFLEAYLAEHSNLAAKP"
     gene            5866..6909
                     /locus_tag="VIL39_11670"
     CDS             5866..6909
                     /locus_tag="VIL39_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5482131.1"
                     /translation="MVHTLFNHKAVDEISSFRDVCIRAYRLMLLARILDDKFASLYRM
                     GKIHGGVFLGRGQEALSVSIGLALRKGDIFAPLIRDGAGRLAFGEPILDGVRTYLGSA
                     LGPMRARDGNIHRGRPKEGLFVMISHLGAMISVVNGALMAHRFQGITGTVGATCIGDG
                     ATSTGAFHEAVNQAAVEKLPLVLIVANNHYAYSTPTERQFACRSLVDKAAGYGISGHS
                     VDGTDLSACLKVVSDAVSQARAGGGPQMVVADLLRLCGHGEHDDASYIDPKLKESSAG
                     RDCLKVAEAYLLDNNWADAQTLAAWHTEMAQKVEESVAKVQREPGPDPYKEDWCALAS
                     KHLSEGHDQGEPR"
     gene            6906..7871
                     /locus_tag="VIL39_11675"
     CDS             6906..7871
                     /locus_tag="VIL39_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5482132.1"
                     /translation="MSITYLEAIREAQARALADDPRVFIYGQDVGAFGGAFKATKNLA
                     QRFPGRVIDSPISEDAMLGLAVGAAIQGMRPIIEMQFADFSTPAFNQIVNQAATLHWR
                     TRVPCPITVRLPSGGTSGSGPFHSQSMEAIYAHYPGLIVMTPATVEDAYSMLLEAVAI
                     DDPVIFCEHKYLYYHLKADRLPTEAMPTGKARIARTGRDMTLVAYSAMVHEALAVAEE
                     LATEGTEIEVVDLRSVKPLDTDTVMASVARTGRLLCVGEAWPWGGVTAEVIARVASEG
                     FDLLDAPPQRLNAKDTPVPYHPNLWAAHRPTARSIAAAARRLLRL"
     gene            7911..9212
                     /locus_tag="VIL39_11680"
     CDS             7911..9212
                     /locus_tag="VIL39_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY5482133.1"
                     /translation="MPRGTHTATHHTPRITHHASRFTFHVSLHPDFMPSIPIIMPQLG
                     ESIAEATVVSLLVPVGEHVQTDEDIIEVETSKATMNVASPCPGRVESFLAKPGESYPV
                     GTVLGYLQASPEDAARLGLDTLPPATDGEETEKSASASRGQADNAQKHVQPTVRGLPV
                     PANAVGASYMSPRMKARMVELGLHAADLAGLPGSGTAGRVTIQDFEKFIANLEKHKLT
                     QASSMRVAVADAMRRSWTRPLATVALPVCFDPLLNHRKTCNPKPGPALYALRALALAL
                     AENSAPAGRLIGNKIVHPSSIDVGFAVEAEDGVLVPVIRNADKRLLKDLVQRYDELVE
                     LARQRKLPADATGGSIATVTNFGTFGLTWATPIPLPEQTLVLGMGAARRVPNWDAAKG
                     QFVPAMEANVTLSFDHRVLDGGAAGRLLARVAQLLASPEKL"
     gene            9684..>10294
                     /locus_tag="VIL39_11685"
     CDS             9684..>10294
                     /locus_tag="VIL39_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5482134.1"
                     /translation="MTLYNLFPLLAGPFSRWGEHFTRATAMGFDWVYVNPIQQLGASR
                     SLYSIADYFRINPALLDPQSAAPPDEQVRQMTVAARAGGLKMMIDLVINHSAIDSQLT
                     REHPEWFVREPDGRVANSFCQHNGEKVVWHDLAQFDHQHTRDPEGLYRYCLSVVEHLL
                     ALGFEGFRCDAAYQVPRSFWHRLIQDIKARHPRTCFVAETLGCS"
BASE COUNT         2009 a         3121 c         3141 g         2023 t
ORIGIN      
        1 aggctgatga tgtatttgat ggcgtccggc accgacattt ccagtttgat cacctttcct
       61 tcgggaacca tcagcagaaa gcccgaggtg ggattggggg tggccggaac aaagacacag
      121 actaccttct ggccagtctt agcctcgacc tcttcctgct ggtcgctggt gatgaacccg
      181 atcgagtaca ccgcgggatg cggaaactcg accagcacga cggtgcgaaa ggcggtcttg
      241 ttgctggagg agaaggcgtc attcacctgc ttggtagcgc cataaatctt gttgagcagc
      301 gggatacgca gcagggccga atccacccat tcgatgatct tgcgaccgaa gtaattccgg
      361 gccagcaatc ccacggtgcc aatcaggaat atcgccagcg ccagcgccac gacgctccag
      421 taccaataca tcggtccgtt ccctgcgtcg tgatgggtta acttggtcgg caggaagatc
      481 agcagcgtat cggtaatgtt ggcgacggtt ccaaacagcc accgcaggac cgcgatggaa
      541 atcacggccg gcaacacaat ggcaaggccc gcccaaaagt tggcctgcca gcgtgcgaaa
      601 aggctcttct tcacttcacc agcttaagcg agacaggaag ccgcctcaag gcaaagagga
      661 agctggcaga atccgttaca catcggttac acgtcggctg ctcttccctt ccatgttcgg
      721 ctgagggcgc ttaagacaaa ccggcgggcc agtcggcgga ccagcgcgtc ttccgccgct
      781 ttcatccgcc gccgagcccg gttgtcctga tcatcgtagc cgagcagatg cagcactccg
      841 tgaacgacgt agcggaccaa ttcgctttgc caggtggtat ggaaacggcg agcctggcac
      901 actgcttcgt ccaggcaggc gacaatttcc ccgtgcagca aagcaggacg gacgtctcgc
      961 cggccccgaa agcggccgct cggcgcgggg aagcgtgacg ggcgggcccc ctgccctgcc
     1021 ctttctgcgt agtcaaaggt aatcacatcg gtcgagcctt tgtgtctcag gaatgcctcg
     1081 ttgaggcgag tcatctcgga tgcggctacg aggtaaatag caagatcgaa gctcccatcg
     1141 cgccacgttt cctcgagcag cgctcgtgcg atccgccgca aaaggcccaa atgcacgcta
     1201 tgaaccgcct gctggttgcg caggtagagt atcctctggc ggtcggggcc cgcgtcatgg
     1261 cctcgaatcg cgcgctcatg cctttcgcct ttgcagtatg gaagcccggt agccattcaa
     1321 gccgtttaat ccttcggcct actccgcgtt cgcagccgga cgaggccgtg actggctgcg
     1381 gtgttcttcg tatgcagcaa tgatgcgctg caccagcgga tgccgcacca catcccgctt
     1441 ggagaattca acaatcgcga tgccttcggt gaacttgagc gcacggtgcg cttcgagcaa
     1501 acccgactgc ttatgcacgg gtaggtcaat ctgcgtttca tcgccggtaa tcaccgcctt
     1561 ggaactaatg cccaaccgcg tcaggaacat gaacatctgc tcagtggttg agttctgcgc
     1621 ctcgtcgaga atgatgaacg cattgttcag tgtacggccg cgcatgtagg ccagcggcgc
     1681 aatctcgatt acgccccgct ccgtttgctt ctggacctcc tcggctggaa gcatgtcgtg
     1741 caacgcatca tgcagtggac gcaagtaagg catgatcttc tcgtataggt cgcccggcag
     1801 gaagccgagc gcttcgccgg cctcgaccgc ggggcgggtc aggatgatgc gcgagacctg
     1861 tccttcccgc aaagcagcga gcgccatggc cacggcgagg taagtcttgc ccgtgccggc
     1921 cgggccaacg ccaatagtca cgtcatgacg ccgaatcgca tcgaggtatt tcttctgtcc
     1981 caccgtcttg gcggtaacgc tgggtttctt ctcggacgtc tgaatgcggt cactcaggat
     2041 gtccttgagc atgggaacgc cctcgtgctt gacgatgttg agggcatggg tgaactcgcg
     2101 gttgcgcacg ggggtgcccg ccttgagcga atgctcgagc agcagaaaca gttgcttggc
     2161 gcggtccacg gcatcgctct cgccatccag cttgatccag ccttcgcgcg aggtggcctt
     2221 gacgccgagt tggtgttcga ccgcctggag attgcggggg tcgttattat agagctgttg
     2281 ggccacccgg gcgttttcga agtggagagt ttcagtgggc atggctaaag gtgtgatacg
     2341 tgatacgtga aacgtgaggc gtgatgcgtg atgcgtgaaa cgaagcaact aatcctcggc
     2401 cagggcggtg cgcagtttgg cggccaggtc ccgcagcgct tccggcacga cgaccgtgtt
     2461 cgccagaacg ggcatgaagt tggtgtcgcc ttgccaacgc ggaacgatat ggatgtgcaa
     2521 atgccccagc accccggcac cggcgacctg cccgaggttc atcccgatgt tgaacccgct
     2581 cggcttcata acacgtgaga gcgcattttg gcagcgccgc gtcagtttca gcaggtccgc
     2641 gagttcctcg tccgtcaagc cgttcagatc ggggacttgt ttgtacggca cgaccatgag
     2701 gtggcctcca gtataagggt aggtattaag cagagcgaag caggtgcggt cgcgcgcgac
     2761 gacgtgattg gcctcgtcat cgctggattg ggcgatgcgc gtgaaaacgc tctcgtcgag
     2821 cgcgggttta ggcgccaaga tatattcgat ccgccacgga gcgtgcagtg attccatgag
     2881 caaagcgtag tgagataacc ggggaatgtc aaaacgggga agtgacgccc gacgggcgac
     2941 agcgggatgg ggaatagcgc ctcgctggac cgccgacttc gcggattgga gccgtccgcg
     3001 cagaacacag acaagcgctt tagccgccag ttaggtgaat tgaacactga gaatcttctt
     3061 cgtggtcagg gcttggcggc gaggttggaa tgctccgcca ggtaggcctc caggaattga
     3121 cggatgcgtt taaactcctc gccgacctga tgtgaaagct cggcggcgct ggtcagtctt
     3181 tcctcaaagc cctgtcgttc gagttcgcgc aacagcggca ccatccgccg catcccgcaa
     3241 gtggcgctag cacccgcgca actatgcgcc agccgtcgca cctcctgagc cgcaccggcc
     3301 tgaacggcgg cttcgagttg ctcgacttgt tgcccggtct ggtccaggta aagagttgcc
     3361 agctcccgca ggttgttctg atcgccgtta gtgaaatcga gcaaccgtgc catgtcaacg
     3421 ggctcgtcct ccggcggcgc ggcagcaggg ctggactggg ccggggcatt agcccccgga
     3481 gggctttggg tgccggtggc cgtggtttct gccggtttgg ttctcccagc caccgcggcc
     3541 caccgctcca caatcctccg cacgtcctcc agccgcactg gcttggcaat ataatcatcc
     3601 atgccggcag ccaggcactt ctcgcggtcg ccctgcattg cactggccgt catggcgacg
     3661 actataatcg aggacttgta atttgggaat tgcgagggtt gctgctgccg ctcgcgaatc
     3721 aggcgcgcgg cttccaaccc gcccatttcc ggcatcatca cgtccatgaa taccaggtcg
     3781 taaggttggc ggtccaacgc ctcgagcact tccaaaccgt tggccgcgag gtcggcgcga
     3841 taccccattt gctggagcag gcggagcgcg accttctggt tgacgatgtt gtcgtcacag
     3901 agcagcatgc gcagaggaaa gcgactggcg cacgtcgggt catgtttggc gctgggagcc
     3961 accgccttga ggacagcggg cttggagccg gacagcgcgc ggagcaaggt ttcgcggagc
     4021 tgggccggct tgattggctt ggtcaggcaa cctgcaatgg aagcgcgggc gaagtcggga
     4081 tgatcggaat gcacgccgat ggaagtcagc agcacgagag gcagagttcc acgacctggc
     4141 agtttgcgga tttcgctggc cagcattagg ccgtccatgt tgggcatttg catatccaga
     4201 atggccaaat cgaaggattc tcccgcgcgc agccattcca gagcctgggc cgcgtcccgg
     4261 gtatcgcgcg ggaccattcc ccacttgcgg gtctgcaatg caagaatgcg gcaattagtc
     4321 ggattgtcgt ccacaatcag caggcgcaaa tcagccagtt gtggcagccg tccttcgagt
     4381 gcatgcagcg gcgcgtctgg cgcggcttgc aggggaagag taaattggaa agtggacccc
     4441 ttccgaggca cactctcgac ccacatcctg ccgcccatca tctcgaccaa ccgcttgctg
     4501 atggccagac cgaggcctgt gccgccatat tggcgtgttg ttgatgcgtc cgcctggctg
     4561 aaggatttga acaagcgggc caactggtcc actggaatcc caatgccgat gtcacgaacg
     4621 aggaaatgaa gttgccacgg ttccgccccg cttgcggttt gttccggcgc ggaaagcact
     4681 ttcacctgga cgaccacttc acccgcggag gtgaatttga tcgcgttgct cagcaagttg
     4741 acgagcactt ggcgcaaccg agtcacatcg ccaaccattt gcgccggaat gccgtcgtcc
     4801 atctggtacg cgacatccag tttcttctcc gccgctttcg gcgccagcag gtcaagcccg
     4861 tcctcaatac acgcgcgcag atcaaagggc tggctctcca gttcgagctt gccggattcg
     4921 atctttgaga aatcgagaat gtcgttaatg atagtaagca gggattcgct gctggaatag
     4981 accgtctcca cgtaaccgtg ctgctcgtgg cttaaggtcg tttccaggag caagccggac
     5041 atagcgatga taccgttcat gggcgtgcgg atctcgtggc tcatattagc caggaattcc
     5101 gccttggcgc gcgtcgcggc ttccgcggcc atacgtgcgg ccaacaactc gcggttggcc
     5161 tgtatcaatt cgtcccgtaa atgcttggtc cgcagcaccg atctaagacg ggcccgcagt
     5221 tcggccggct caaagggttt ggtcaggtaa tctaccgccc ccaactcgaa cccccgcaac
     5281 ttgtccgttg tgctgttcca ggcggtcagg acaatgacgg ggatggtttg agtttcaggg
     5341 gactctttga gctgccgcaa taactccatc ccgttgacgc ccggcaatcc aaggtcgagc
     5401 aagatcacat cgaactgctt ctcacgcgcc agcttcaacc cggtggccgc atcgctggca
     5461 ctggagagag ttatgttgtc atcttgcagg agcccggaaa ggacctccgg cattcgggga
     5521 tcatcttcga ccaacaagac ctgggacgca ggcagattca tagtctggca atctcaataa
     5581 ttagcgtttg ctctgtccga acggcattaa atcggtcttt gttaagcaca ttcaacacca
     5641 atcccggccc cgaaccaatc tcattctttc gctgatccag cctggcgcgg cccgcgttgg
     5701 tttttggacg aatacccagg cgccccgctt cgccttggcc tgagccctcg gcgcaacacg
     5761 cgttcggccc cggaacaaga cgaattgggc gggatttttg gacacgcctg aaacgcatga
     5821 agaaacggtt gccaggaggc cctgcaggcg gttagactgc ggcctatggt tcacactctg
     5881 ttcaaccaca aagcggttga cgagatttcc tcattccgtg atgtgtgcat aagggcttac
     5941 cgccttatgc tcctggcgcg catcttggat gacaagttcg cgagcctcta tcgcatgggc
     6001 aaaatccatg gcggcgtgtt cctcggacgc ggccaggaag cgttgagcgt gtccattgga
     6061 ctggccttgc gcaagggtga catcttcgcc ccgctgattc gggacggggc cggacggctg
     6121 gcttttggcg agccaattct ggatggtgtg cgtacttatt taggttcggc cctggggcca
     6181 atgcgcgcgc gcgatggcaa cattcaccgg ggccggccca aggaggggtt gttcgtgatg
     6241 atcagccacc tgggcgcaat gatttccgtc gtgaacggcg cacttatggc ccatcgcttc
     6301 caaggaatca ccggaactgt cggggcaacg tgtattggcg acggcgccac ttccaccggc
     6361 gcctttcacg aagccgtgaa ccaggccgcc gtcgagaaac tgccgctggt gttgatcgtg
     6421 gccaacaacc actacgctta ctccactccc accgagcgac agttcgcctg tcgctcactc
     6481 gtggacaagg ctgccgggta tggcatcagc ggacactccg tggatggcac cgacttgtcc
     6541 gcctgcctta aagtggtcag cgacgcagtc agccaggcaa gggcaggtgg cggcccgcaa
     6601 atggtcgtcg ccgacctgct gcgcctctgt ggccacggcg aacacgatga cgccagttac
     6661 attgatccga agctgaaaga atcttctgcg gggcgggatt gtctgaaggt agctgaagcc
     6721 tatctgttgg acaacaactg ggcagacgcg caaacccttg cagcctggca caccgagatg
     6781 gctcaaaagg tcgaggaaag cgtcgccaag gtacagcgtg aaccggggcc tgacccgtac
     6841 aaggaagact ggtgcgccct cgcctcgaag catttgagcg aagggcacga ccagggtgaa
     6901 ccgagatgag cattacttac ctggaggcaa ttcgggaggc gcaggcacga gcgctggcgg
     6961 acgatccgcg cgtgtttatt tacgggcagg atgtaggcgc tttcggcggt gcgttcaagg
     7021 caaccaagaa tctggcgcag agattccccg gccgggtaat agactcgcct atcagcgaag
     7081 atgcgatgct cgggctggcc gtgggcgctg ccattcaagg aatgcgaccg attatcgaaa
     7141 tgcagttcgc ggatttctcc acgcccgcct tcaaccagat cgtcaatcag gcggccaccc
     7201 tgcattggcg aaccagggtg ccctgcccta tcacggtgcg cctgcccagc ggcggcactt
     7261 cgggcagcgg ccctttccat agccagagca tggaggccat ctacgcgcat tatccgggcc
     7321 tgatcgtcat gacgcccgcg accgtggagg atgcttacag catgttgctc gaagctgtgg
     7381 ccattgatga cccggtcatc ttctgcgagc acaaatacct ttattaccac ctgaaggccg
     7441 accggttgcc aactgaggcc atgccgaccg gcaaggcgcg gattgcgcgc acagggcgcg
     7501 acatgactct ggtggcctac agcgccatgg tgcacgaggc gctggccgtc gcggaggaac
     7561 tggccacgga aggcactgag attgaagtcg ttgacctgcg ctcggtgaaa cccctggaca
     7621 cggataccgt gatggcctcc gtagcccgca ccggccgttt gctttgtgtg ggtgaagcgt
     7681 ggccttgggg cggcgttacg gcggaggtta tcgcgcgcgt cgccagcgaa ggctttgacc
     7741 tgctcgacgc cccaccgcag cggctcaacg ccaaagacac accggtcccg tatcacccca
     7801 atctctgggc ggcgcatcgc ccgaccgcgc gcagcattgc cgccgccgcg cgccggctgt
     7861 tacgcttatg aaaacggagt gcgggagaat tggagtgttg gagcgttgcc atgcctcgcg
     7921 gcacgcacac cgccacccat cacaccccac gcatcacgca tcacgcatca cgtttcacgt
     7981 ttcacgtttc actccatcct gatttcatgc cttccattcc catcatcatg ccacaactgg
     8041 gcgagtcaat cgccgaagcg actgtggtta gtctgctggt cccagttggc gagcacgtgc
     8101 agacggacga ggacatcatc gaggtcgaga ccagcaaagc gacgatgaat gtcgcttcac
     8161 cctgcccagg ccgggtggag agctttctgg caaagcccgg tgagagctat cccgtcggga
     8221 cagtgctggg ctacttgcaa gccagcccgg aggacgcagc gcgcctgggt ttggacacgc
     8281 tgccgccggc aacggacggg gaggaaaccg aaaagagcgc gtcggccagc aggggtcagg
     8341 ccgacaatgc gcaaaaacac gtccagccaa cggtgcgcgg gctgcccgtt ccagcgaatg
     8401 ccgtcggggc aagctacatg tccccgcgca tgaaagcgcg catggtggaa ctaggcctcc
     8461 atgctgcgga cctggcgggc ctgcccggca gcggcaccgc cgggcgcgtc acgatccagg
     8521 atttcgagaa gttcatcgcc aatcttgaga aacacaagtt gacccaggcc tcgagcatgc
     8581 gcgtggcggt cgccgatgcg atgcggcgca gttggacgcg gccgctggcg acggtcgctc
     8641 tgcccgtgtg ttttgacccg ctcctgaacc accgcaaaac gtgcaatccc aagcccgggc
     8701 cggcgctgta tgcgttgcgc gcgctggctt tggcgctcgc cgagaacagc gctcccgccg
     8761 gacggctcat cggaaacaag attgttcacc cgtcctcgat tgatgtgggg tttgccgttg
     8821 aggcggaaga tggcgtgctg gtgcccgtga ttcgtaatgc cgacaagcgg ctgctcaaag
     8881 acctggtcca gcgctacgac gagctggttg agctggcgcg ccagcgcaag ctgccggccg
     8941 acgctacggg cggctccatc gccaccgtga ccaactttgg caccttcggc ctcacctggg
     9001 cgacgcccat tcctctgcca gagcagacgc tggtgctggg aatgggcgcg gcccggcgcg
     9061 tgccgaattg ggacgcggcg aaaggccagt tcgtgccagc catggaagcc aacgtgacgc
     9121 tcagtttcga tcatcgcgtc ctcgatggcg gcgcggctgg ccggttgctc gcccgcgtgg
     9181 ctcaactgct cgccagtcct gagaagctct aacctctgac aaccagttgg ctctgggctg
     9241 tcgcggggcg agcaaaccag gacagatttt gtagttagca aaccaggaat ggaatctcac
     9301 ggagagcccg attctattgg ggatttgatc cagagacgcc acccgcgccg tctattcgtg
     9361 ctggcgggcg agcggcggag acgggctttg aacctccagt tcgcgtctcc gccggtcgca
     9421 acgcgtaacg gactcgcccc gccagcacga ataggcgcgc ctgagatcaa cgccccccag
     9481 cgggacgata agcgcgtctg tgcatgggga gggagccttc tcaagcccca ggaccccagc
     9541 aatgcagcga aaacgcgaaa accgcagcaa accgcgatca ttcctggttt gctaactaca
     9601 gaaaattcct gctttgaaaa ctacgcgaca cccggcgcaa cgattcttgt gaccgcgaag
     9661 cgatgtcgta tattcttctg cctatgacgc tctataacct tttccctctc cttgccgggc
     9721 cattctctcg ctggggcgag cattttaccc gggcgacggc gatgggcttc gactgggtgt
     9781 acgtcaatcc catccagcag ctgggcgcct cacgcagcct ttattcgatt gccgactatt
     9841 ttcggatcaa cccggccctg ctggacccgc agagcgctgc cccgcccgac gaacaggtcc
     9901 gccagatgac tgtggccgcc cgtgccggtg gcttgaagat gatgattgac ctcgtcatca
     9961 accactcggc cattgattca cagttgacgc gcgagcaccc ggagtggttt gtccgggagc
    10021 cggatggccg ggtcgcgaac tcattctgcc agcacaacgg tgagaaagtc gtctggcacg
    10081 acctggccca gttcgatcac cagcacacgc gcgacccgga agggctatac cgctattgcc
    10141 tcagcgtggt cgagcatctg ctggcgctcg ggttcgaggg cttccgttgc gacgccgcct
    10201 atcaggtccc gcgcagtttc tggcatcggc tcatccagga catcaaggcc cggcatcccc
    10261 gcacctgttt tgtcgccgaa acgctcggct gctc
//
LOCUS       DATCGS010000569         5295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_46586, whole genome shotgun sequence.
ACCESSION   DATCGS010000569 DATCGS010000000
VERSION     DATCGS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46586"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1449
                     /locus_tag="VIL39_11690"
     CDS             193..1449
                     /locus_tag="VIL39_11690"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5482135.1"
                     /translation="MAKLTVKDLNVRGKRVFMRVDYNVPMEEKDGQMVITDATRIVAT
                     LPTLRLLIGQGAKLILTAHLGRPKGKREPSMSLRPVAVKLEELLGRPVAFADDCIGPK
                     VEQAVSVMKPGDVLLLENVRYYKEEEANDPAFAEKLAKVADVYVNDAFGAAHRAHAST
                     EGVARLVAKRGGQCAAGLLMELELKFLGDELEKPARPFVVILGGAKVSDKIQVIDRLL
                     EKADALLIGGAMAYTFRLAQGYKTGKSLVEPDKTDIAKAALAKAKQRGVKFLLPVDDV
                     VAVPVKTDKLDKKGKPVIDYQNVRVNTDMNCPDDAAGLDIGPATVKAYCGEVAKAKTI
                     LWNGPMGLFEDKRFAEGTFAVARAVAEVTQKSGAKSIIGGGDSVKALNKAKLGDKVTF
                     MSTGGGASLEFLEGQVLPGVAALSDK"
     gene            1508..2272
                     /gene="tpiA"
                     /locus_tag="VIL39_11695"
     CDS             1508..2272
                     /gene="tpiA"
                     /locus_tag="VIL39_11695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5482136.1"
                     /translation="MNKERKLIIAGNWKMNKTVAEALDLVRSLRIELANVKEVDIVVC
                     PPFTALSEVSKAILDSNIRLGAQNMSEHKGGAYTGEIAAGMLKEFSVRYVILGHSERR
                     QYQKEPDALIARKAAAVHAASLKPIVCVGETLAEREAGQMQKVLETQVRGSLAGLSKE
                     QMVETIVAYEPVWAIGTGKTATTAQAQETHAFIRGVLEKMFEEAVARRVRIQYGGSVK
                     PSNAHELMSQADVDGALVGGAALEARSFTDIIKNSI"
     gene            2284..2736
                     /gene="secG"
                     /locus_tag="VIL39_11700"
     CDS             2284..2736
                     /gene="secG"
                     /locus_tag="VIL39_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY5482137.1"
                     /translation="MAFLIPLFTVVLVLDCAALILLVLVQLPKKDAGAGLAFGGGATD
                     ALFGAGSGNVLTQITKYAATAFFVLSLVLSVMQRSYHTRTTSAFEKDLMKPAASSTVP
                     STALPPTATKPAVVPGTNATITIPPRAEATNLPVAVPSSPAPTAAPKQ"
     gene            2946..4229
                     /locus_tag="VIL39_11705"
     CDS             2946..4229
                     /locus_tag="VIL39_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5482138.1"
                     /translation="MQHQLNRRQFLKAASATALATPFLLHAAGAATARKGPNDRITMG
                     FIGTGTQGRGLLNNFLAMPDTQVVAVCDVDTTRREHARKVVEEFYSIKGNKEFKGCTG
                     YKEFGDLLARKDIDAVVVAAPDHWHAYIAIAACKAGKDVYCEKPLSLTIHEARAMVKA
                     ARKYDRVFQTGSMQRSSNEFRKACELVRNGRIGKVKQVIVDVGPPSKPCDLGEEAMEP
                     GLNWDLWLGPSPMRPYNSVLSPRGVHTHFPDWRNYREFSGGMMTDWGAHHFDIAQWGL
                     GMDESGPVEIIPPEDPKATRGVRYLYANGVEMIHGDSGGVLFIGSEGKILVNRGKFEA
                     TPASLAEAPLGDNAIHLYKSYSHTKDFLDCMRSRKKPICDVEIGCRSVTVCHLGNLAY
                     WNHRRLRWDPVKEQFVGDKEANQWLDRPKRGGWKV"
     gene            complement(4239..4940)
                     /gene="radC"
                     /locus_tag="VIL39_11710"
     CDS             complement(4239..4940)
                     /gene="radC"
                     /locus_tag="VIL39_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY5482139.1"
                     /translation="MRLKDQPASERPRERLVTLGADALSPAELVAILLRTGLKGANVL
                     EIAKQLVQKYGSLQALAQAPIKDLQTVKGIGRDKAATLAAAFSLARRMAKELRGESPV
                     LDTPEAVANLMREDVRLRNVETFQVLLLNTRRRLIDVVKVSDGTLDTILVHPREVFKP
                     AIAANAAAIILLHNHPSGEATPSEADIKVTRDLIRAGQLLKIEVLDHIILGRATQERP
                     KDYMSLRELGYFAGC"
     gene            complement(5078..>5295)
                     /locus_tag="VIL39_11715"
     CDS             complement(5078..>5295)
                     /locus_tag="VIL39_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEY5482140.1"
                     /translation="FPRVNWLTLLRCGPGLLLRGRLPASCVEVFQAESFTLRSPSATA
                     LEADGELIGHLPATFSIARSRLRVIVP"
BASE COUNT         1117 a         1559 c         1698 g          921 t
ORIGIN      
        1 ggagcgccga cattcttgtc ggcgtgttcc catattgccc atgcgcggcc acacgaacag
       61 gccgacaaga atgtcggcgc tccccccaac gactcgcgaa tgcgtccgag ccaaaccgat
      121 tgaaccgatt gaatttgact cgccccaccc ctcaggtatg ctctgcggcc cgaatctgga
      181 agaaaggctt ttatggcaaa actgacagtt aaagatttga acgtgcgagg caagcgcgtg
      241 tttatgcgcg tggattacaa cgtgccgatg gaagagaaag acggccagat ggtcatcacc
      301 gatgcgactc gcatcgtggc gacgctgccg acgctgcggc tgctgatcgg gcagggcgcg
      361 aagctgattc tgacggccca cctgggccgg cccaaaggca agcgcgagcc gtccatgtcc
      421 ctgcgaccgg tggcagtcaa gctggaggag ctgctcggcc gccccgtggc gttcgctgac
      481 gactgcatcg gcccgaaggt cgagcaggcg gtcagtgtga tgaaaccggg cgatgttctc
      541 ctgctggaaa acgttcgtta ttacaaagag gaagaggcca atgacccggc gtttgccgag
      601 aagctggcca aggtggccga cgtttatgtc aacgacgcgt tcggcgcggc ccatcgcgcg
      661 catgcctcca cggaaggcgt ggcccggttg gtggccaaac gcggcggcca gtgcgcggcg
      721 ggtctgttga tggagctcga gctgaagttc ctgggcgacg aactggaaaa gccggcccgc
      781 ccgtttgtcg tcattctcgg cggagccaag gtctcggata agattcaggt tattgatcgg
      841 ctgctcgaaa aggcggacgc gctcctcatt ggcggcgcga tggcctacac gttccgtctg
      901 gcgcagggct acaagacagg caaatcactc gtcgaaccgg ataagacgga catcgccaag
      961 gccgctctgg ccaaagccaa acagcgcggg gtgaaattcc tgctgccagt ggatgatgtg
     1021 gtcgcggtgc cggtcaaaac cgacaagctg gacaagaagg gcaagccggt cattgattac
     1081 cagaacgtgc gcgttaacac ggacatgaac tgcccggatg acgcggccgg actcgacatc
     1141 ggcccggcaa ccgtgaaggc ctattgcggc gaggtggcca aagccaagac cattctatgg
     1201 aatggcccaa tgggtctatt cgaggataaa cgatttgcgg aaggcacctt cgccgttgcc
     1261 cgcgcggtgg cggaggtgac gcagaaaagt ggcgccaaga gcatcatcgg tggcggcgac
     1321 agcgtaaagg cgctgaacaa agccaagctg ggcgacaaag tgacctttat gagcaccggc
     1381 ggcggcgcga gcctggaatt cctcgaaggc caggttctcc ccggcgtggc agcgttatcg
     1441 gataagtaac ccaggaagaa cttaacgcaa agacgcaaag tagcaaaggc gcaaggataa
     1501 ctgatttatg aataaagaac ggaaactgat tattgccggc aactggaaga tgaacaagac
     1561 ggtcgcggag gcgctcgacc tggtgcgcag cctgaggatc gaattggcca acgtcaaaga
     1621 ggtggacatt gtcgtctgcc cgccattcac cgcgctgagc gaggtctcga aagcgatcct
     1681 cgattccaac atccggctgg gtgcgcagaa catgagcgag cacaagggcg gcgcctatac
     1741 cggcgaaatc gcagccggaa tgctcaagga attctcggtg cgctacgtca ttctcggtca
     1801 ctccgagcgg cggcaatacc agaaggaacc ggacgcgctg attgcccgta aagccgcggc
     1861 cgtccacgca gcctcgctca agccaattgt ctgcgtcggg gagacgttgg cggaacggga
     1921 agcgggccag atgcagaaag tcctggaaac ccaggtgcgg ggcagcctgg ccgggttgag
     1981 caaagagcag atggtggaga ccatcgtcgc ctatgaaccg gtctgggcga tcggcaccgg
     2041 caagacggcc accaccgccc aggcgcaaga gacccacgcg ttcatccgcg gggtgctgga
     2101 aaagatgttc gaggaagctg ttgcccggcg ggtccgcatc caatatggtg ggagcgttaa
     2161 gccgtccaac gcgcacgagc tgatgagcca ggcggacgtg gatggcgccc tggtgggcgg
     2221 agccgcgctt gaagcgcgca gtttcactga tatcattaaa aattcgatct agagcaagaa
     2281 tatatggctt ttctaattcc cctattcacg gtggtgttgg tgttggattg cgcggccctg
     2341 attctcctgg tgctggtcca gcttcccaag aaagacgcgg gcgcgggcct ggctttcggc
     2401 ggcggggcta cggatgccct gttcggcgct ggttcgggca atgtcctgac ccaaataacc
     2461 aagtacgcgg ccacggcctt cttcgttctg tcgctggtgc tctcggttat gcagcgctcc
     2521 tatcataccc ggaccacgag cgcctttgaa aaggatctga tgaagccggc cgcgtcctcg
     2581 actgttccct cgaccgcgct gccgccgacg gccaccaagc cggccgtggt cccgggcaca
     2641 aacgccacga tcaccatccc gccgcgggcg gaagccacca atctgcccgt tgccgttccg
     2701 tcaagtccgg cgcccacagc ggcaccgaag cagtagtagt tctcaaacgg ctgagtcgga
     2761 gcgctggcag gtcgttgggg caggccattc acgcccccaa gcaggcaagt tgccggccat
     2821 ctcaggggta tgcaggcaaa tgcgttgaac ccagcgggcg cgggttggct ggccgcgtag
     2881 ctctccaatc tgcctcaaga atttgattgc cggggggtca agggaattag acaatgcgta
     2941 acgcaatgca acaccaacta aatcggcgtc aattcctgaa agcggcttcc gccaccgcac
     3001 tcgccacacc cttccttctt cacgctgccg gtgccgccac cgcccggaaa ggtcccaacg
     3061 accgcatcac catgggcttc attggcacgg gcacgcaggg gcgcgggctc cttaacaact
     3121 tcctggcgat gcccgatacg caggtggtgg ccgtgtgcga cgtggacacg acgcggcgag
     3181 agcacgcccg gaaggtggtc gaggagttct acagcatcaa gggcaacaag gaattcaagg
     3241 gctgcacggg ctataaggag tttggcgacc tgctggcacg caaggacatt gatgccgtcg
     3301 tggttgccgc gcccgatcac tggcacgcct acatagccat cgcagcgtgc aaagcgggca
     3361 aggacgttta ttgcgagaag ccgctgtcct taaccatcca tgaggcccgt gccatggtga
     3421 aggccgcccg gaaatatgac cgcgtcttcc agaccggctc gatgcagcgg tcctcgaacg
     3481 aattccgcaa agcgtgcgaa ctggtgcgaa atgggcgcat tggcaaagtc aaacaggtca
     3541 tcgttgatgt ggggccgccc tcgaagccct gcgacctggg agaagaggcc atggagccgg
     3601 gcctgaactg ggacctatgg ctgggaccgt cgccgatgcg gccttacaat tctgtgctca
     3661 gcccgcgcgg cgtgcatacg catttccccg actggcggaa ctaccgcgag ttttccgggg
     3721 gcatgatgac ggattggggc gcccaccact ttgacattgc ccaatggggg ctaggcatgg
     3781 acgagagcgg cccggtggag atcattcccc ccgaggaccc caaggccacc cgcggcgtgc
     3841 gctatctcta cgccaacggg gtggagatga ttcacggcga cagcggcggc gtgctcttca
     3901 tcggcagcga gggcaagatc ctggtcaacc gcggcaagtt cgaggcaact cccgcctccc
     3961 tggctgaggc gccactcggt gataacgcga tccaccttta caagagctac agccacacca
     4021 aggactttct ggattgcatg cgcagccgca agaagccgat ctgcgatgtg gaaatcggct
     4081 gccgctcggt gaccgtctgc cacctgggca acctcgctta ctggaaccat cggcgtttgc
     4141 gctgggaccc ggtcaaggaa cagttcgtgg gcgataagga agccaaccaa tggctggacc
     4201 gtccgaagcg cggtgggtgg aaggtgtgat cgcgcggttc agcacccagc gaaataaccc
     4261 agttcccgca acgacatata gtccttgggc cgttcctgtg tggcacggcc caggatgatg
     4321 tggtcgagca cctcgatctt caggagctgg ccggcgcgga tcaagtcgcg ggtcaccttg
     4381 atgtcagcct cagagggcgt cgcctctccg gaaggatgat tgtgcagcaa gatgatcgcc
     4441 gccgcgttgg ccgcgatggc cggcttgaac acctcgcgcg ggtgaaccag gatcgtgtcg
     4501 agcgtcccgt cggacacctt caccacatca attagcctcc gccgggtgtt gagcaggaga
     4561 acctggaacg tctcgacgtt gcgaagccgg acgtcctcgc gcataagatt cgcgaccgct
     4621 tccggggtat ccaacacggg agattcgccg cgcagctctt ttgccatcct ccgggcgagt
     4681 gagaaggccg ccgcgagtgt tgctgccttg tcgcggccga tgcctttgac cgtctgcaaa
     4741 tctttgatcg gggcttgagc cagggcttgc agggaaccgt acttctgaac caactgcttg
     4801 gcgatttcca gcacattggc tcctttcaga cccgtgcgca gcagaatggc caccagctcg
     4861 gcaggactga gggcatccgc gccgagggtg acgagccgct cgcgcggacg ttcgctggca
     4921 ggttgatcct tcaaacgcat cccagtcatg cgagggagac tgtatgacaa ggatgggcaa
     4981 tagcacgaaa aaacatcggg gcgtcctcct gtgaatcagc cggaagggcg taagcgaaac
     5041 gtggcggcac tccgaccgcc atgcgcccgg ctcggggtta cggcacaatg acgcgcagcc
     5101 gggagcgtgc gatggaaaag gtggccggca ggtgcccgat gagttcgcca tcggcttcca
     5161 gggcggttgc tgaggggctc ctcagggtga aggattcggc ctgaaagacc tccacgcacg
     5221 aagccggcag ccggccacgc agcaacaggc cgggcccgca gcggaggagc gtgagccagt
     5281 ttacccgcgg aaaaa
//
LOCUS       DATCGS010000570         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_494851, whole genome shotgun sequence.
ACCESSION   DATCGS010000570 DATCGS010000000
VERSION     DATCGS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494851"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VIL39_11720"
     CDS             <1..1320
                     /locus_tag="VIL39_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5482141.1"
                     /translation="LCLCGCTASHYRTSADKQVYDAIARRTPLVTNMEPRFRIEHTNA
                     LVLDGLPQVTVTNDFLGEAAAAELGAHVFSLQETLKIAVQHSRVYQNDREQLFLTALS
                     LTLSQHQFAPLFPGVTIVVPSRIMHEYVWNISRRVDSDIIGTVPWYPEMRNHPVARGR
                     FFTDADMESSASVCVLGADMAQALFPLDSPLGRDVRVGGDYYRVIGVMEPRGKGVKTD
                     ETRPGAKTAAQEMFIPLETAKTRFGEVLVKFRSGSLSAERVQLHEVTVKVASREEVLP
                     VAEAVKAVLKHHHKKKDYEVVVPLELLKRAERTKQIFNIVLGSIAAISLLVGGIGIMN
                     IMLATVTERTREIGIRRALGAKRRDIITQFLVEAVMLSGVGGLFGVALGITIPLLVTW
                     FADMKTIITFWSPLIAFSISGLVGVAFGLYPALRAAKMDPVEALRHE"
     gene            complement(1338..>2588)
                     /gene="rho"
                     /locus_tag="VIL39_11725"
     CDS             complement(1338..>2588)
                     /gene="rho"
                     /locus_tag="VIL39_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5482142.1"
                     /translation="NIAKLQAMSMPDLNQMARDLGVENFGTMRKHEVIFHILQKNAER
                     AGVLFSEGVLEVLPEGFGFLRSQSFNYLPCPEDIYVSPSQIRRFDLQTGNLVAGQIRP
                     PKEKERFFALLKVEAVDQEDPDKAKDKTHFDNLTPLFPNRRFILETTPDELSTRVLDL
                     VCPIGKGSRGLIVAPPRTGKTVLMQKLANAMLKNNPETYLFILLIDERPEEVTDMERS
                     CRGAEVISSTFDEPPERHVQVAEMVIEKARRMIEHKRDVVILLDSITRLARAYNTVQP
                     HSGKILSGGVDANALHKPKRFFGAARNIEEGGSLSIIATALVDTGSRMDEVIFEEFKG
                     TGNMEICLDRALVDRRIFPSINIERSGTRKEELLYHPEEYNKVVILRRALTGVPPVEA
                     MELVLGKLKQTGSNVEFLLGMSVG"
BASE COUNT          476 a          822 c          777 g          513 t
ORIGIN      
        1 ctctgtctgt gcggctgcac ggctagccac tatcgcacct cggcggacaa gcaagtctat
       61 gacgccatcg ctcgaaggac gccgctcgtc accaacatgg agccgcgctt taggatcgag
      121 catacgaatg cccttgttct ggacgggctg ccgcaagtaa ccgtgacgaa tgactttctg
      181 ggcgaggcgg ccgcagcaga gctgggcgcg cacgttttct cgctgcagga gacactgaag
      241 attgcggtcc agcatagccg cgtttaccag aacgaccggg agcaattatt cctgacggcg
      301 ttatcgctga cgctctccca acaccagttc gcgccgctct tccccggtgt caccatcgtg
      361 gtaccgagcc gtatcatgca tgagtatgtc tggaacatca gccgccgggt ggattccgat
      421 attataggca ccgtgccgtg gtatccggag atgcgcaacc accctgtcgc tcgcgggcga
      481 ttcttcactg acgcggacat ggagtccagc gccagcgttt gcgtcctcgg ggccgacatg
      541 gcccaagccc tgtttccgct cgattcacct ctcggccgtg acgtgcgcgt gggcggtgac
      601 tactaccgcg tcattggcgt aatggagccg cgtggcaagg gtgtgaagac cgacgaaacc
      661 cggcctgggg caaagaccgc agcgcaggag atgttcatcc ccctggaaac ggccaagaca
      721 cgctttggcg aggtgctcgt gaagttccgg agcggcagcc tctcggccga gcgcgtgcag
      781 ttgcatgaag tcaccgtcaa ggtcgccagc cgcgaggagg tcctcccggt tgcggaggcg
      841 gtaaaagcgg tgctgaagca ccaccacaag aagaaagact atgaggtcgt ggtaccgctg
      901 gaattgctca agcgcgccga gcgcaccaag caaatcttca acatcgtgct gggttccatc
      961 gccgccatct ccctgcttgt cggcggcatc ggtatcatga acatcatgct ggcgaccgtc
     1021 accgagcgca cgcgtgagat cggcatccgc cgcgccctgg gcgccaagcg gcgcgacatc
     1081 atcacgcagt tcctggtgga agcggtgatg ctctccggcg tcggcggcct gttcggcgtc
     1141 gcgctgggga tcaccattcc tctgctggtg acctggttcg ccgacatgaa gaccatcatc
     1201 accttctggt cgccgctcat cgccttttcc atctccggcc tggtcggcgt ggcgttcggc
     1261 ctctaccccg ccctgcgcgc ggccaaaatg gacccggtcg aggcgctgcg gcacgagtag
     1321 ccgcaggccc cggaagctca acccacactc atgccgagca ggaactcgac attgctgccc
     1381 gtctgcttga gcttgcccaa caccagttcc atcgcttcga ccggcggcac gcccgtgagc
     1441 gcgcggcgaa ggataacgac cttgttgtat tcctccgggt ggtagagcag ttcctctttg
     1501 cgcgtgccgg aacgctcgat gttgatggag gggaaaatgc ggcggtccac cagcgcgcgg
     1561 tccaggcaaa tctccatgtt gcccgtgccc ttgaactctt cgaagatcac ttcgtccatg
     1621 cgcgaaccgg tatccaccag cgccgtcgca ataatggaca gcgagccgcc ttcctcaata
     1681 ttgcgggccg caccgaagaa gcgcttcggc ttgtggagcg cgttcgcatc cacaccaccc
     1741 gacaggattt tgccggagtg cggctgcact gtgttatagg cgcgcgccag gcgcgtaatg
     1801 gaatccagga gaatgaccac gtcgcgcttg tgctcgatca tgcgtcgggc cttctcgatc
     1861 accatctccg ccacctgcac atgccgctcg ggcggctcgt cgaacgtcga gctgatgact
     1921 tcggccccac ggcaggatct ctccatgtcc gtgacttcct cgggccgctc gtcaatcagc
     1981 aggatgaaaa ggtaggtctc gggattgttc ttcagcatcg cgttggcgag cttctgcatc
     2041 aagaccgttt tgccggtgcg cggcggcgcc acgattagcc cgcgcgaccc tttgccaatc
     2101 gggcagacca ggtcgagcac ccgcgtggac agctcgtcgg gggtggtctc caggatgaag
     2161 cgcctgttcg gaaacagcgg cgtcaggttg tcgaagtgag tcttgtcctt agccttgtcc
     2221 gggtcttcct gatccaccgc ctcgactttc agcagcgcga agaaacgttc tttctccttg
     2281 ggcggccgga tttggcccgc caccaggttg cccgtttgca gatcaaaccg gcgaatctgg
     2341 gaaggtgaga catagatgtc ctccgggcag ggcagataat tgaagctttg cgagcgcagg
     2401 aagccgaagc cctcgggcag gacttccaac accccctccg agaacaacac cccggcgcgc
     2461 tccgcattct tctgcaggat gtggaagatc acctcgtgct tgcgcatggt gccgaaattc
     2521 tccacgccca aatcccgcgc catctggttc agatcgggca ttgacatcgc ctgcagtttg
     2581 gcgatgtt
//
LOCUS       DATCGS010000571         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_393964, whole genome shotgun sequence.
ACCESSION   DATCGS010000571 DATCGS010000000
VERSION     DATCGS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393964"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..333
                     /locus_tag="VIL39_11730"
     CDS             31..333
                     /locus_tag="VIL39_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482143.1"
                     /translation="MKNAVQLKKPFGKATHSCVRNARYLGWEDAFDVEFEDGLCFLEP
                     HATIRKANRISAKAVPVSVEVDDDLGSHFKVTYDTGEVAEVSWAFIRELPPTKRGC"
     gene            343..2742
                     /gene="priA"
                     /locus_tag="VIL39_11735"
     CDS             343..2742
                     /gene="priA"
                     /locus_tag="VIL39_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5482144.1"
                     /translation="MIARVTLEIALRKEFDYLIPPELVDQVDVGSRVQVPFGSRKVLG
                     CVTGLAEESARASLKPILKVIGAQTLVTPKILKLARWIGEYYCCAPETALKSVLPEAV
                     RHEQANWRERLHVRALPVPGELPKLPARQKEVWRILQERPELPLSELLALAKTTAATV
                     RRLEDRGLVTVAAEISERDPYAREHILPSQPLPLNPAQANALAAIITAMDVGKSEVQS
                     QGGEVQSPKSKVQSPGGAGVQGSKFEVQGSMFDVGCSMLDVQASAPAPPASSPVPRSS
                     TFLLHGVTGSGKTEVYLQAIAHALEQGKGAIVLVPEISLTPQTVERFKARFSSGPLQT
                     LVAVLHSHLSAGERHDEWHKIRQGRARIAIGARSAIFAPVEPLGLIVVDEEHEHSYKQ
                     EEAPRYHARDVAIVRGQMEGAVVVLGSATPSLESYYNCGKGKYTLLRLPERADDKKLP
                     VVRIVDMRQTVRPGKGVPLFSPQLKEAIARRVEQKEQVILFLNRRGYSTSLQCPLCGY
                     VAECPNCSVSLTYHRHENKLCCHICAHVAPVPAVCPNEKCRNPAIRYSGVGTQKVEAT
                     LAKLFPQARVVRMDSDSLKRKEDYRRILGDFRVGKIDILVGTQMIAKGLHFPNVTLVG
                     IIYADLALHQPDFRAGERTFQLLTQVAGRAGRGDIEGEVFVQAFTPFHPAIQFARRHD
                     FEGFYEQEMEFREQLKYPPVGRIALLTLKGRNEEKVKFSADYLKHELEKAVGSIKDLV
                     IAGPAPAPLAKAETYYRYQIMLRAPRMATLSQRLALLIRSLTLPEDVTLAVDIDPVNL
                     N"
     gene            complement(2950..3507)
                     /locus_tag="VIL39_11740"
     CDS             complement(2950..3507)
                     /locus_tag="VIL39_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY5482145.1"
                     /translation="MIEAVWPRPGIGREGALILGGSLLIALAAQLQVLLPFSPVPVTG
                     QTFAVLFLAAIYGSKRGPAAVITYLTLGLSGLPVFAGGAFGAARLFGPTAGYLAGFLG
                     AAFIVGILSERGWDRRPWSTAASMIIGNLVIYIAGAAWLFKFAGLKSVLSVGVLPFLA
                     GDAIKIALATLLLPVGWKLIGRFLR"
BASE COUNT          768 a         1216 c         1141 g          683 t
ORIGIN      
        1 cattcatgat ttgaagcagg aaagacgcct atgaagaacg cggttcaatt gaagaagcct
       61 ttcggcaaag caacccactc ctgtgtgcga aacgcccggt atttgggttg ggaggacgcc
      121 ttcgatgtcg aattcgagga cggcctttgt tttctcgaac cccatgccac catccgcaaa
      181 gccaaccgga tttctgcgaa ggccgtcccg gtcagtgtgg aagtggatga cgacctgggc
      241 agccatttca aagtgaccta tgacactggc gaggtggctg aggtttcctg ggccttcatc
      301 cgcgaactgc cacccacaaa acgaggctgc tagtcggcga ccatgatcgc ccgcgtcacc
      361 ctggaaatcg ccctccgcaa ggagttcgat taccttattc cgcccgaact ggtggaccag
      421 gttgacgttg gcagccgcgt ccaggtcccg ttcggctcgc gcaaggtcct tgggtgcgtg
      481 accgggctgg ccgaggaatc agcgcgcgcc agcctgaagc ccatcctgaa ggtcatcggc
      541 gcgcagacac tggtcacacc caaaatcctc aagctcgccc gctggatcgg ggagtactac
      601 tgttgcgcgc cggagacggc cttgaaaagc gtcctgcccg aagccgtccg ccacgagcaa
      661 gccaactggc gcgaacggct gcatgtccgc gccctgccgg tccccggcga attgcccaag
      721 ttgcccgcgc ggcagaagga agtctggcgc attctccagg aacgacccga gttaccgctg
      781 agcgaattgc tggccttggc aaagaccacg gcggcaacgg ttcgccgcct ggaagaccgg
      841 ggcctggtta cggtcgccgc ggagatttcc gagcgcgacc cctatgcgcg cgagcacatt
      901 ctgcccagcc aaccactgcc gctgaacccc gcgcaagcca acgcactcgc cgcgatcata
      961 acggccatgg atgtgggaaa gtctgaagtc caaagtcagg gaggcgaagt ccaaagtcca
     1021 aagtccaaag tccaaagtcc cgggggggcg ggagttcaag gttcaaagtt cgaggttcaa
     1081 ggttcgatgt tcgatgttgg gtgttcgatg ttggatgttc aagcttccgc ccccgcccct
     1141 ccagcctcgt cccccgtccc tcgctcctcg accttcctcc tccacggcgt cactggcagc
     1201 ggcaagaccg aggtctatct gcaagccatc gcccacgccc tggaacaggg caagggcgcg
     1261 attgtcctcg tcccggaaat ctcactcacg ccccagaccg tcgagcgctt caaggcgcgc
     1321 ttcagttccg gtccgctgca aacgctggtg gcggtgttgc acagccattt gtccgccggc
     1381 gagcggcacg acgagtggca taagattcgc cagggccgcg cgcggattgc catcggcgcc
     1441 cgctcggcca tctttgcgcc cgtcgaaccg ctcggcctca tcgtggtgga tgaggaacac
     1501 gagcattcct acaagcagga ggaggcgccc cgctaccacg cgcgcgacgt cgccattgtg
     1561 cgcggccaga tggaaggcgc ggtggtagtg ctcggctcgg ccacgccttc gctggagagc
     1621 tattacaact gcggcaaggg caaatacacg ctgctccggc tgcccgagcg ggccgacgac
     1681 aagaagctgc ccgtcgtgcg catcgtggat atgcgccaga ccgtgcgccc aggcaaaggg
     1741 gttcccctct tctcgccgca gctcaaagag gcgattgccc ggcgggtgga gcagaaggag
     1801 caggtcatcc tcttcctgaa ccggcggggc tactccacct ccctgcaatg ccccctctgc
     1861 ggctacgtcg ccgaatgccc gaattgcagc gtctcgctca cctaccatcg ccacgaaaac
     1921 aagctctgct gccacatctg cgctcacgtc gctccggtgc cggccgtctg cccgaacgag
     1981 aaatgccgca acccggccat tcgctactcc ggcgttggca cgcagaaggt cgaggccacc
     2041 ctggccaaac tgttcccgca ggcgcgggtt gtgcgcatgg actccgactc cctgaagcgg
     2101 aaggaggatt atcgccgcat cctgggcgac ttccgcgtcg gcaagataga cattctggtc
     2161 gggacgcaga tgatcgccaa gggcctgcat ttccccaacg tcacgctggt cggcattatc
     2221 tatgccgacc tcgccttgca tcagccggat ttccgcgccg gcgagcgcac cttccaactg
     2281 ctgacgcagg tggccggacg cgcgggccgc ggtgacatcg aaggcgaggt gtttgtccag
     2341 gcgttcactc cctttcatcc ggcgattcaa ttcgcccggc ggcatgactt cgagggcttt
     2401 tacgagcagg agatggaatt ccgcgagcaa ctgaaatacc cgcccgttgg ccgcatcgcc
     2461 ttactgacgc tcaaagggcg caacgaggag aaggtgaagt tctccgctga ctacctgaag
     2521 cacgagttgg agaaggcggt gggcagcatc aaggacctgg ttatcgccgg cccggcgccc
     2581 gcgccgttgg cgaaggccga gacctactac cgctaccaga tcatgctgcg cgcaccacgc
     2641 atggcgacgc tcagccagcg cctggcactg ctcatccggt cgctgacgct gccggaggac
     2701 gtcacgctgg cggtggatat tgacccggtg aatttgaact gatgtcgaga tagaaacccc
     2761 agttgcccgg cgtccccctc gcagatagct gacgcttcta ggccggcact gcgtcccgca
     2821 tgggctgtgt gccgggcacg ggcaaatctt tcccgctcaa acgcaactct tgaacctcct
     2881 cgcggaccag agcgcacaac tggccgtaag cctgctcctc agtttccccg tgtcagaagg
     2941 cgtggattcc taccgaagga agcggccgat gagcttccag ccaaccggaa ggaggagggt
     3001 agcgagggcg atcttgatgg catcgcccgc aaggaaagga aggaccccga cgctcaggac
     3061 cgacttcaac ccggcgaact tgaacagcca ggcagcccca gcgatgtaaa tgaccaggtt
     3121 tccgatgatc atgctggcgg cagtgctcca aggccggcgg tcccatcctc tctcgctaag
     3181 tattccgaca atgaaggctg cgccgagaaa gccagccagg taaccggcgg ttggcccgaa
     3241 aagcctcgct gccccaaagg ctcctccggc gaataccggg agtccgctca aacctagagt
     3301 gaggtaggtt atgacagcag ccggaccccg cttgctgccg tagatggcgg ccaggaataa
     3361 gaccgcgaag gtttggcctg taacgggcac gggtgagaag ggcaacagga cttgaagctg
     3421 agcggcaaga gcaattaaaa ggctgccccc caggatgagg gcgccttcgc gaccgatgcc
     3481 gggcctcggc caaacggcct cgatcatgac tctgtgtcgg atacaagtag cttccattag
     3541 tgatgctttt ctggccacgc cgcttaacac caaagctccg gcacagccgc cagagccagt
     3601 cgtggcctgc aatgatgatg tccggatttc ataaatcggt caactggcaa acgcgaccgc
     3661 ttttgctctt gcagcggggc ttgtattcaa cagatgtcga ggtagggtga tcaaatcgtg
     3721 ccgttcggcg ataagctctg gctcgtcccg ttgccggcgt tgtgggcgcc ataaaatcat
     3781 taaccccttt ccgaagaaac tccgattc
//
LOCUS       DATCGS010000572         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_394103, whole genome shotgun sequence.
ACCESSION   DATCGS010000572 DATCGS010000000
VERSION     DATCGS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394103"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VIL39_11745"
     CDS             complement(<1..1638)
                     /locus_tag="VIL39_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY5482146.1"
                     /translation="MKPRFLSLLLLIACGLFLASPAHSAQKPTKADIAQIFAEAATYE
                     PGQSREPFRRIEELVGQPSRGARKQLEAGLVRLLAPSATFEARRFACKQLGIIGSSRA
                     LPALGNLLKNEETAGIACLALTTYSPGKADKLLRAALPAARGAARIQIINTLGDRRDA
                     KAVKLLAQAAADADLTVAQAAMGALGKVGNRAAWKAIASCSKAANPALQTALTEATVR
                     CADALAGSGDRKTATPIYEGLLAPAQPDYVRRAAFDALLRLDNARARQRILAILHGAD
                     STLKPVAITKVRTLPAKNASEVFAAELPRLQPQEQVWMIDSLAARGDGPACAAIGNSL
                     TSPEANVRRAAINTLGRIGGAWCVPLFVAALNQSKDVEEWRALESSLISLRGDAQTDS
                     AIVAALKESSGNTRANLITALARRHGAEANTVLLAETGQSDPTVAKAALRAISKTATW
                     KEATPLLTLLTQTRDAEVRSEAGGAAAQAIAGIDDPSQRSTLVREALGRAQDLDSHVV
                     LLGLLPGCGDATALATLKIAATSTNTRIRDAVVRALAD"
     gene            complement(1689..>3154)
                     /locus_tag="VIL39_11750"
     CDS             complement(1689..>3154)
                     /locus_tag="VIL39_11750"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5482147.1"
                     /translation="AGEPHWSCRMNPAFRLAQPPALVVVSGYAREKLSTHSCVYKLSS
                     RWDHRNVFETVMKLQAASQFSRRQFLKRATTAVALPLIVPGSVLGLNGAVAPSNRIVF
                     GSIGVGNRARDTLPTFLSFAEVQFLAMSDCRADRLKSAKETVDRHYGNQDCTAYPDFR
                     DLLARKDVDAVSIATGNRWHGLGSIYAARAGKDIYCEKPVTLTIGEGRALVDTCRRFG
                     TIYQAGTQRRSTASYRFAMEMVRQGKIGRVHTIEMQVWAGPGIAHEKVAPVPAGWNYD
                     IWLGQSPWYPFVPARVNAWQYFWDTAEGMMTDMGCHYTDQMQWALDTDRTGPVEFEAK
                     GDLPDPTKFCSDTPITGVGRCTYANGVTGVMYQRGAFKDRYLRYIGTEGWIQVDDETD
                     LVTAEPKSILSLKGGSGAGWGDTSDHVRNLLNCIRSRTPTTCNPEVAHRAITICQAMT
                     ISLRLGRKLKWNPVKERFNDEEANRMLYREPRAPWRV"
BASE COUNT          524 a          970 c         1088 g          572 t
ORIGIN      
        1 gtcagccagc gcgcggacga cggcgtcccg gatgcgggtg ttagtactag tggcagcgat
       61 tttgagcgtg gcgagggccg tggcatcacc gcagccgggc aacagtccca gtaaaacgac
      121 gtggctgtcc aagtcctgcg cgcgacccag ggcctcgcgg acgagggtcg agcgctggct
      181 ggggtcgtct attccggcga tcgcctgagc ggcggcgcct cctgcttcgg aacgcacctc
      241 cgcgtcgcgg gtttgagtca gcagcgtgag cagcggggtg gcctccttcc aggtggcggt
      301 cttcgagatg gcgcgcaagg ccgctttggc gactgtcgga tcggactggc cggtctcggc
      361 cagcaacacg gtattcgcct ccgcgccgtg gcgtcgggcc agggcggtaa tcagattagc
      421 gcgcgtattg ccggacgact ccttcagcgc cgcgacaatt gcgctgtcgg tttgcgcgtc
      481 gccgcgcagg ctgatgaggg aggattccag ggcgcgccat tcttcaacgt ccttcgattg
      541 attcagggcc gccacgaaca acgggacgca ccaggcgccg ccgatgcggc cgagggtgtt
      601 aatggccgcg cgccgaacgt tggcctccgg cgacgtcagg ctgttgccga tggccgcgca
      661 ggccgggccg tcgcctcggg cggccaggct gtctatcatc cagacctgct cctgaggctg
      721 gaggcgggga agctcggcgg cgaacacttc cgaggcgttc ttggctggca aagtgcgaac
      781 cttggtgatg gcgaccggct tcaaggtgga gtctgccccg tgcaggatcg cgaggatgcg
      841 ctgtcgggcg cgggcattgt ccagacgaag caacgcgtcg aaggccgccc ggcggacgta
      901 gtccggctgg gctggcgcga gcagcccctc ataaatcggc gtcgcagtct ttcgatcacc
      961 cgagccggcc aacgcgtcgg cgcaacggac cgttgcttcg gtcagggcgg tttgaagggc
     1021 cgggttggcg gctttggagc atgaagcaat cgctttccag gcagcccggt tgccgacctt
     1081 tcccaacgct cccatagcag cttgcgcgac ggttaagtcg gcatcagccg cagcctgcgc
     1141 cagcagtttc accgccttcg catcccggcg gtcgcccagc gtgttgatga tctgaatgcg
     1201 agccgcgccc cgtgccgccg gcagcgcggc gcggagaagt ttgtcggctt tgccaggcga
     1261 atacgtcgtg agggcaaggc acgcgatgcc cgcggtttcc tcgttcttca atagattgcc
     1321 cagggccggc aaggcgcggc tgcttccgat aatgcccagt tgtttgcagg cgaagcggcg
     1381 cgcctcgaag gtggcgctgg gcgcgagcag ccgcaccagg ccggcttcaa gctgcttgcg
     1441 cgcgccgcgg gaaggctggc ccaccagctc ttcgatgcgc ctgaacggct cgcggctttg
     1501 gccgggctcg taggtggcgg cctcggcgaa gatctgggcg atgtcggctt tcgtgggctt
     1561 ttgggccgaa tgcgccgggg acgcgaggaa gaggccgcaa gcgatgagca gcaggaggct
     1621 aaggaatcgt ggtttcataa gcagggaatg gcaaattgaa aatggaacat ggcgaatggg
     1681 acatcgtgct agacccgcca gggggcgcgc ggttcgcggt agagcatgcg attcgcctcc
     1741 tcgtcattaa agcgctcctt gaccgggttc catttgagct tgcggccgag ccgcaggctg
     1801 atggtcatcg cctggcaaat cgtgatcgcg cggtgtgcga cctccgggtt gcaggtggtc
     1861 ggcgtgcggc tgcgaataca gttgagcagg ttgcgcacgt ggtcggaggt gtcaccccag
     1921 cctgcgccgc tcccgccttt gaggctgagg atggacttcg gctcggcggt cacgaggtcg
     1981 gtctcgtcat ccacttgaat ccacccctcg gtgccaatat agcggaggta acggtctttg
     2041 aacgcccccc gctgatacat gaccccggtg acgccattgg cgtacgtgca acggcccacg
     2101 ccggtgatcg gcgtgtcgct gcagaatttt gtggggtccg gaagatctcc cttggcctcg
     2161 aactccaccg ggccggtgcg gtcggtgtcg agcgcccact gcatctggtc ggtgtaatgg
     2221 cagcccatgt ccgtcatcat gccttcggcg gtgtcccaga aatactgcca cgcattgacg
     2281 cgggcaggca cgaacggata ccacgggctt tgacccagcc agatatcgta gttccagccc
     2341 gccggcacgg gcgccacctt ttcgtgcgca atgcccggcc cggcccaaac ctgcatctcg
     2401 attgtgtgca cgcgtccgat tttgccctgt cgcaccatct ccatggcgaa gcgataagag
     2461 gccgtcgagc ggcgctgggt ccccgcctgg tagatggtgc cgaagcggcg gcaggtgtcc
     2521 accagtgcgc ggccctcgcc gatggtgagc gtgaccggtt tctcgcagta aatgtccttg
     2581 cccgcgcggg cggcgtaaat ggagcccagg ccatgccagc ggttgccggt ggcaatcgag
     2641 accgcatcca catccttgcg cgccagcaaa tcgcggaagt ccgggtaggc ggtgcaatcc
     2701 tggttgccgt aatggcggtc caccgtttcc ttcgcgctct tcagccggtc ggcccggcaa
     2761 tcgctcatcg cgagaaactg cacctcggca aacgagagga aagtcggcaa ggtgtcccgg
     2821 gcgcgattgc cgaccccgat ggagccgaag acaatgcgat tgctcggggc gaccgcgccg
     2881 ttcaggccga gcaccgaacc gggcacgatg agcggcaggg cgacagccgt ggtggcgcgt
     2941 ttcaggaact ggcggcggct gaattgggat gcggcttgta gtttcataac agtctcaaag
     3001 acgttgcgat ggtcccagcg ggaggaaagc ttgtaaacac aagaatgtgt ggacagcttc
     3061 tcccgggcgt atcctgacac caccaccaag gcgggcggct gtgccaatcg gaacgccgga
     3121 ttcatccggc agctccaatg aggctcgccg gcct
//
LOCUS       DATCGS010000573         5753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_98447, whole genome shotgun sequence.
ACCESSION   DATCGS010000573 DATCGS010000000
VERSION     DATCGS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98447"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VIL39_11755"
     CDS             complement(<1..755)
                     /locus_tag="VIL39_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482148.1"
                     /translation="MQAWPCRVFSLFLVMLIFDQLKKDDLQLRLVAMAVLGGLGVLLG
                     GLWWVQVVNARDYQANLETQSFRTVRIPAGRGKILDRNGLALAENQPTYNVSLYLEEL
                     RSQFREEYERSRPVRTITNSGAFGLRWLGSPTVTTQYVRLKKAQMDVLGRQARYSVAS
                     NVVAHISQRLQLPLSLNATNFERHYQTRLALPCPVLTDVTPAQIARFEEQSTSPMGVD
                     LEVQPTRWYPYGTTAAHVLGHLRRDDDSKEGEEA"
     gene            complement(808..1359)
                     /locus_tag="VIL39_11760"
     CDS             complement(808..1359)
                     /locus_tag="VIL39_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482149.1"
                     /translation="MNWLNTAFVLGAAFLAVFGEAAFGGLRHLVGAQIDLLPPLMVYA
                     GLCTGITTVALLAVCAGLWFDSLSANPLGVTVLPLFAIGLAIHLKRELILRDQTFAQL
                     ALGLAGSMATSLLTLLLLLSMGRNPLLGWGTLWQIVVVSIGGAIATPIWFALFGWLQR
                     TLVHHRAVESSFRPDRQIRRGRL"
     gene            complement(1356..2099)
                     /gene="mreC"
                     /locus_tag="VIL39_11765"
     CDS             complement(1356..2099)
                     /gene="mreC"
                     /locus_tag="VIL39_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5482150.1"
                     /translation="MNLPSQTVARFKLGIGSLFLPLFGLAASTQQVADAAGDAALPRR
                     ELLKLNEGLHRENQQLRLQALQAQETLSENARLRQLVGWQRQAPWKLKLAKVVLREPS
                     NWWRTVQIDLGSRDGLRVNLPVRTMDGLVGRISSVSLTRSQVVLLGDPNCKVSARVEN
                     EARDTGIIGPSGPLDTEFVVLGYLSKNANLKPAQSVVTSGEGGIFPKGIPIGKIVDEQ
                     TVDYGLRTEARVRLAANLNALEEVWVMFP"
     gene            complement(2265..3290)
                     /locus_tag="VIL39_11770"
     CDS             complement(2265..3290)
                     /locus_tag="VIL39_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5482151.1"
                     /translation="MFAQFKSLFSNDIGIDLGTANSVVYVRDRGIVLREPSVVAIQAG
                     TTNVLAVGEEAKRMLGRTPGNIVAIRPMKDGVIADFEITEAMLRHFIQKVHHRKLIAP
                     RVVVAVPSGITEVEKRAVKDSATHAGAREVYLIEQPMASAIGVGLPVHEPAGNMIVDI
                     GGGTCEIAIISLAGIVFSRSLRVGGDEFDETIVAHIKRAYNLMIGERTAEEIKIRIGS
                     AFPLEQELTMEVKGRDLSAGLPKTLTIRSEEIREALQEPLSSILESVRITLERCPPEL
                     SADLVDRGIVMAGGGSLLRGIDRLVAEETGLPVHIADDPLSAVAEGTGRVLQELQFLK
                     RVSSNSK"
     gene            complement(3423..4667)
                     /locus_tag="VIL39_11775"
     CDS             complement(3423..4667)
                     /locus_tag="VIL39_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5482152.1"
                     /translation="MVDYDRTAVTYRYECWRALPSGVIEAAGTIFLLLIAVRGYQAGP
                     FAKAMVAGGGSIGLILAPLVVSRVEASGWPVAKAAGRLAALGALSFLVMALVPMLPVY
                     VIGCVVAMTTSSAAIPLLTQMYQENYPERERGRRFARTMMIRIATAAVFSDLAGRALS
                     QHLDQFRWLLLIFAGALAFASFCLERCPSQPLTASGGTHPFHALRYARDDRLFRQTLI
                     AWMFLGFALLMMAPLRVEYLANAKYGVRWHGQLLTAGTVALLTGVIPNIARLILNPVW
                     GWLFDHMNFFILRLTLNLGFALGILSFFVSGSVAGLVAGAVFFGISNAGADVAWGLWV
                     TKFAPPERVADYMSVHTFFTGVRGVVAPLVAFYLVSDLPLNVLGWISVGLIGVGSAFL
                     VPEIKFGKGARRGVALMEEISE"
     gene            complement(4894..5346)
                     /locus_tag="VIL39_11780"
     CDS             complement(4894..5346)
                     /locus_tag="VIL39_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482153.1"
                     /translation="MDDGTGNHVYRNTLTGGKGFSTIQFPTLGLALPANSFEISTVVH
                     GVSLTGAANYNYTAGIAFLSSSTQSSGPSGLHVADLNLSSVSSGANSGRMRLVEWIGT
                     TAVVRPSSTQTSQPQVANFSLSNSYLLDVKGLCLSHLRWRCWRWGRYF"
     gene            5518..>5753
                     /locus_tag="VIL39_11785"
     CDS             5518..>5753
                     /locus_tag="VIL39_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482154.1"
                     /translation="MNIATEPRTCPSCGAVIPAKAPQGICPKCLMAAALATDAGQTGG
                     KRPEPPSLERLAAAFPQLDVIEFIGQGGMGAVYKA"
BASE COUNT         1214 a         1837 c         1663 g         1039 t
ORIGIN      
        1 gcctcctcgc cttccttgga atcgtcgtcg cgtcgcagat gacccagaac gtgtgccgcc
       61 gtggttccgt aaggatacca gcgcgtcggt tggacctcca gatcaactcc catcgggctc
      121 gtggactgct cctcgaagcg ggcgatttgg gctggggtca catccgtcag caccgggcag
      181 ggcaaagcaa gacgcgtctg gtaatggcgt tcgaagttgg tcgcgttcag tgagagcggc
      241 agttgtagcc gctgactgat atgtgccacc acgttgctgg cgacggaata gcgggcttgg
      301 cgacccaaga catccatttg cgccttcttg agcctgacat attgcgtcgt gaccgtcggg
      361 gagccgagcc agcgcaaccc aaaggccccg gagttggtga tcgttcttac gggccgactt
      421 cgttcgtatt cctcccggaa ctggctccgc agttcttcga gatacagact cacgttgtaa
      481 gtgggctggt tctccgccag cgcaaggccg ttgcggtcca ggatcttccc gcgccccgcc
      541 ggaatgcgca ccgtccggaa cgactgtgtt tcgaggttgg cctggtaatc ccgggcgttt
      601 accacctgca cccaccacaa tccgcccagc aacacgccca aaccgcccaa gacagccatt
      661 gcgaccaacc gcagttggag atcgtccttt ttgagctggt cgaagatcaa cataaccagg
      721 aacaggctaa agacacggca aggccaggct tgcaccccgg cgcctcctgc cttcggactt
      781 tagaccttag actttggact ttagacttca cagccgcccc ctccggattt gccggtctgg
      841 ccggaagctg ctttcgacag cgcggtggtg gaccagcgtg cgctgcagcc agccgaacag
      901 agcaaaccaa atcggtgttg cgatggctcc gccgatgctg accacgacga tctgccacaa
      961 cgtgccccag ccgagcaacg ggttgcggcc catgctcagc aacagcagca gcgtcagtaa
     1021 cgacgttgcc atactgcccg ccagtccgag ggccaactgg gcgaaggtct ggtcacgcaa
     1081 gatcaactcg cgtttcaggt gaatcgccag cccgattgcg aacagcggga gcacggtcac
     1141 gcccagtgga ttggccgaaa gcgagtcgaa ccaaagcccc gcgcaaaccg cgagcagcgc
     1201 cacggtggta atccccgtgc ataggccggc ataaaccatg agcggcggca gcaaatcaat
     1261 ttgtgccccc accagatggc gcaggccgcc gaacgcggcc tccccgaata ccgccaggaa
     1321 ggctgcgcct agaacgaacg ctgtattcag ccagttcatg ggaacatcac ccacacctct
     1381 tccagcgcgt ttagattcgc cgccagcctc acgcgcgcct cggtgcgcaa gccgtagtcc
     1441 accgtttgct catccacgat cttgccaatt gggatgccct tcggaaaaat ccccccttcg
     1501 ccgctcgtca cgacgctttg cgcgggcttg aggttcgcgt tcttggaaag gtagcccagc
     1561 acgacaaatt cagtgtccaa aggtccggaa ggtccgataa tgccggtgtc gcgcgcctcg
     1621 ttctccaccc gcgcggagac cttgcagttg ggatctccca gcaacacgac ttgcgaacgg
     1681 gtgagactaa cggatgagat gcgcccaacc aggccatcca tggtccgaac cggcagattc
     1741 acccgcaggc cgtcccggct gccgagatca atttggacgg tgcgccacca gttggagggc
     1801 tcgcgcagga ccaccttcgc cagcttgagc ttccagggcg cctgacgttg ccagccgacg
     1861 agctgtcgca gccgggcatt ctcgctgagg gtttcctgcg cctgcaacgc ctgcaaccgc
     1921 agttgttggt tttcccggtg cagaccctcg ttgagcttga gcaattcccg ccgcggcagg
     1981 gcggcatcgc ctgcggcgtc ggccacctgc tgcgtggaag cagccaggcc gaacagcggc
     2041 agaaacagac tgccaatgcc cagcttgaaa cgggcaaccg tttggcttgg caggttcaaa
     2101 gtaagcagcg tcaacagcac aaccagaccc aaagcaatgt aatgcggccg tcttagcatc
     2161 taaataatcg gcatttagca gtcagtgact ggcaacaggc ggctggtcgc taatgaccaa
     2221 tcacccttta cggattgcca gctactacga atgacaggag ggaactactt ggaattggaa
     2281 gaaacgcgct tgagaaattg gagttcctgc aagactcggc ctgtgccctc ggcaacggcg
     2341 ctcagcgggt cgtcagcgat atgaaccggc aatccggtct cttcggccac caaccggtca
     2401 atgccgcgca acaacgatcc accgcccgcc atgactatgc cacggtccac caggtcggcg
     2461 gacagctcgg gcgggcagcg ttcgagggtg atgcgcacag attccaaaat gctcgacagc
     2521 ggctcttgca aggcctcgcg aatctcttct gagcgaatgg tcagcgtctt cggcaacccc
     2581 gcgctcaaat cgcgtccctt aacttccatg gttaattcct gctccagcgg aaaagcagag
     2641 ccaatacgta tcttgatctc ttccgccgtg cgttcgccta tcattaggtt ataggcccgt
     2701 ttaatgtgcg ccacgatcgt ctcgtcaaac tcgtccccgc ccacccgcag gctgcggctg
     2761 aagacgatgc cggccaggga aatgattgcg atttcgcaag ttcccccgcc aatatccacg
     2821 atcatgttgc ccgccggttc atgcaccggc aaaccgacac caatggccga agccatcggt
     2881 tgttcgatca aatacacttc gcgcgctccg gcatgagtag cagagtcttt aactgcccgc
     2941 ttttccactt ccgtgatgcc tgaaggaacc gcgaccacga cccgtggcgc aatcagcttc
     3001 cggtggtgga ccttttggat gaagtggcgc agcatcgctt cggtgatctc aaaatcggcg
     3061 atcacgccat ctttcatggg ccgaatggcc acgatgtttc caggggtgcg cccgagcatc
     3121 cgcttggctt cttcgcctac ggcgagcacg ttggtggtcc ccgcctggat tgcgacgacc
     3181 gagggctctc gcaagacaat cccccgatcc ctcacgtaca cgacagaatt tgccgtcccg
     3241 aggtctatcc caatgtcatt ggaaaacagg ctcttaaatt gcgcaaacat gaattttgcc
     3301 taataaaccg acaagttaat ggagacgctg cgctgcggtc aagaatatta aatgaccttt
     3361 ctcagggctg ccaggacgca ccttcccata aatctgcgct acgtccgcgc ccgcgatcag
     3421 agctattcgg agatttcctc catcagggcg acgccccggc gggctccttt gccaaacttg
     3481 atttccggca ccagaaacgc cgagccgacg ccaattagcc cgacgctgat ccaccccagc
     3541 acattgagcg gcaggtctga gaccagatag aatgcaacca gcggcgccac cacgccgcgc
     3601 acgcccgtaa aaaacgtgtg caccgacatg taatcggcca cccgctccgg cggcgcgaac
     3661 ttggtcaccc acaaccccca cgccacgtcg gccccggcgt tagaaatgcc gaaaaacacc
     3721 gctcccgcca ccaatccggc cacgctgccg ctgacaaaga acgataatat ccccagcgcg
     3781 aaccccaggt tcagcgtcag gcgcaggatg aagaagttca tgtggtcaaa gagccagccc
     3841 cagaccgggt tcaggatcag ccgcgcgata ttggggatca cacccgtcag cagggcaacc
     3901 gtgcccgccg tcagcaattg tccgtgccac cggacgccat acttggcatt ggccaggtac
     3961 tcgacgcgga gcggcgccat cataagcaac gcgaaaccca ggaacatcca cgcaatgagc
     4021 gtctgccgga acaggcgatc gtcccgcgcg taacgcaacg cgtgaaacgg gtgggtgccg
     4081 cccgacgcgg tcagcggctg ggagggacag cgctccaggc agaagctggc aaacgcaagg
     4141 gcgccggcaa agatcagcag cagccagcgg aattggtcga ggtgctggga cagagcccgc
     4201 ccagccaagt cactgaagac cgccgcggtg gcgatgcgga tcatcattgt gcgcgcaaag
     4261 cgccttccac gttcgcgctc cgggtagttc tcctggtaca tctgtgtcag caacggtatg
     4321 gcggcggaag aggtggtcat cgccaccaca caaccgataa cgtacactgg cagcatgggg
     4381 accagtgcca tcaccagaaa gctgagggca cccaacgcgg ccagccgccc cgccgccttg
     4441 gccaccggcc atccagaggc ttctacgcgc gataccacca acggtgccag aatcaggcct
     4501 atactgccgc cgccggcgac catcgccttg gcgaacggtc ccgcctggta gccgcgcacc
     4561 gcgatgagca gcaggaatat cgtgcctgcc gcctcaataa cgccggacgg aagtgcgcgc
     4621 cagcattcgt agcgataggt tacggcagtg cgatcgtagt caaccatcgc ccaggagatt
     4681 aacgattaag ggccgactcg aaagtcagaa gttccaggca ggaacccaaa gggcgcatca
     4741 gtcgcgggag gtgggcggcg gcaccaggcg gtgtggtgct tgtggagcca ctcgcggatg
     4801 gcgcggtcca tgccgatgtc gaagccggcg cgtcttcttg actccatggc cagacagggt
     4861 ggggctagcg tagcccgcgg ccgcgccatc ttgctaaaaa tagcgccccc atcgccaaca
     4921 gcgccagcga agatggctca ggcacagtcc cttgacgtcg agcaggtaac tattgctcaa
     4981 gctaaaattg gcgacctggg gctggctcgt ttgggtcgag gaaggtctga caaccgcagt
     5041 cgtgccgatc cactccacga gccgcatccg gccgctattt gccccgctcg acacgctgct
     5101 gagattcaaa tcggcaacgt ggaggccact cggaccgctt gactgggtgc tgctgctgag
     5161 gaacgcgatg cccgccgtat agttatagtt agctgcgcca gtaaggctaa ccccatgcac
     5221 cacggtcgag atttcaaagc tgtttgccgg gagggccaaa cccagcgtcg ggaactgtat
     5281 cgtgctgaat cccttgccac cagtcagcgt gttccggtac acgtggtttc ccgtgccgtc
     5341 gtccactacg gcccaagtgc ttgatgtcgc gccgatctcg cttggcgtaa aaccgggcac
     5401 gctgccgccg actggcaatg aacgggggtg tgacctgctc gaacgtgggt tgtttcttcg
     5461 acagggagcc aggaagtctg taatgtcggc ctgcggacgc cttatctata ggtaggtatg
     5521 aatattgcga cagagcctcg aacttgtccc agttgcggcg cggtcatccc ggcgaaggcg
     5581 ccacaaggca tttgccccaa atgcctcatg gcagcggcgc ttgccacgga tgccggccaa
     5641 accggtggca agcggccgga gccgccctcg ctcgaacggc tggcggcggc cttcccgcaa
     5701 ctggacgtaa tcgagtttat cggccagggc ggcatgggcg cggtctataa ggc
//
LOCUS       DATCGS010000574         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_394326, whole genome shotgun sequence.
ACCESSION   DATCGS010000574 DATCGS010000000
VERSION     DATCGS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394326"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..>2674)
                     /locus_tag="VIL39_11790"
     CDS             complement(312..>2674)
                     /locus_tag="VIL39_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY5482155.1"
                     /translation="AVVAAKPLGVTVDVVPMGVMRANDVSVQKLQLPPKLKEGQVFEA
                     KIFVQADRATPATVRLFRDDQFLGEQKVELSAGKNLFTFPQTLSKPNFYKYDVQVDAP
                     GDPLPQNNRATSFTTVRGEPRILVVSADPEQDKQLAAALKTPSLGVVLVGIKDFPSTL
                     AEMQSYDAIFISNLAAGDLGLDRQRLLESVVRDFGVGLVCVGGDQTYAAGGYRGTPLE
                     STLPVSMELDSKKVLPSGAVVLVMHGMEFANGNEVARMCAQGVLAALGPTDEMGVVLW
                     DGTERWVFDLQKVGDKKKLAAAIAGMNQGDLSSFQGVIQQAHQALQKSTANLKHIIVF
                     SDGDPGAPTAELMQAIVDDRITVSTVLISGHSPPDTMEWMAEQGKGRFYNVNSPDDLP
                     QIFIKETAVILKSAIYEDPFQPQLRSASELVRGIGASEYPKLLGYVATTPKPRAETPL
                     WTDKGDPLLAHWQYGLGRAVAFTSDAKARWARIWLNWDKYRQFWSQIGQWSMRRLENA
                     DFTTEVVTDKGDGLINVEALDEQGNFRNFLNLQAIVASPKGERQTVRLEQTGPGHYEA
                     RFPTKEVGAYLLNLMDVKDGKVRGSQVIGASVNYSPEFNTGEPNLNLLRRIAETGGGK
                     MLDPMVPTVNPFLHDRQKTFQPRDLWESLLKFAIILFLLDVGVRRIQIGRDEWLQGMR
                     VLRSWLFFWRAAPRPPEAEESLAALLSRREQVRSQQTGPAVEPRPELFRPEQPATLPL
                     PGEEGGATPLSQPAPAPTEPSKPPDESAPSTASRLLEAKRRAQKRK"
BASE COUNT          467 a          834 c          870 g          503 t
ORIGIN      
        1 agcaaactgc ggtcccacca gagcgaccaa cgccgccgtc cgggaatcca agggacggtc
       61 agaaaacgcc aacagcccag ccctgctcgt tccgtccggg aaaagcacgt cagaacgcag
      121 tcggtcctcg gagaaaccaa cgcgcaacaa gccctcacgt acggggcgaa gctctgtcgg
      181 cagtatgagc acggttcgtc ttgaaattac aattatccag gaccaaatgc caacacgaat
      241 aacttttgag gaatgccgag ctgccggcat tgatctgcgg ctccaactct gatattgaga
      301 ccgtttgccg cctacttgcg tttctgcgcg cgacgtttgg cttcgagcag gcgactggcg
      361 gtgctggggg cggactcgtc aggcggtttg gacggttcgg tcggcgcggg ggcgggttgc
      421 gacagtggag tagcgccgcc ttcttcgccg ggtaacggca gggtggcggg ctgttcgggg
      481 cggaacaact cgggcctggg ttcgacggct ggaccggtct gctgcgagcg gacctgctcg
      541 cgccgcgaca gcaacgctgc cagcgattcc tcggcctccg gcggacgcgg cgcggcgcgc
      601 cagaagaaaa gccagcttcg cagaactcgc atgccctgca gccactcatc gcgcccaatc
      661 tggatgcgcc gcacacccac atccaacagg aacagaatga tcgcaaactt gagcagcgac
      721 tcccagaggt cgcgcggctg aaaggtcttc tgccggtcat gcaggaaggg attcaccgtc
      781 ggcaccatgg ggtccagcat cttgccgccc ccggtttcag caatgcggcg caggaggttg
      841 agattcggct ccccggtgtt gaactccggc gagtaattga cgctcgcgcc gatgacctgc
      901 gagccgcgca ccttgccgtc tttgacatcc atcaggttca gcaggtaggc gccgacctct
      961 ttggtgggga accgcgcctc gtaatggccc ggcccggttt gttcgaggcg caccgtctgc
     1021 cgctcgcctt tggggctggc gacgatggct tgcaggttca ggaagttccg gaagttgccc
     1081 tgctcgtcca gggcctcgac gttgatcagc ccgtcgccct tgtcggtgac gacttcggtc
     1141 gtaaagtccg cattctccag gcgccgcatg ctccattgcc caatctggga ccagaactgg
     1201 cggtacttgt cccagttcaa ccagatgcgg gcccaccggg ctttggcgtc ggaggtgaaa
     1261 gccaccgcgc ggcccaggcc atattgccaa tgggccagca gcgggtcgcc tttatccgtc
     1321 cacaaagggg tctcggcgcg cggcttgggg gtcgtggcga catagccgag cagcttcgga
     1381 tactcgctgg cgccgatgcc gcgcaccagc tcgctcgccg accggagctg cggctggaag
     1441 gggtcttcgt agatcgcaga tttcaggatg acggcggtct ccttgatgaa gatctggggc
     1501 agatcgtcag gagagttgac gttgtagaat cggcctttgc cctgctcggc catccattcc
     1561 atggtgtccg gcggggaatg gcccgagatg agcacggtgc tgacggtgat ccgatcgtcg
     1621 acaatagcct gcatcaattc cgcggtcggc gcgccggggt cgccgtcgct gaagacgatg
     1681 atatgcttga ggttggcggt ggacttctgg agcgcctgat gggcctgctg tatcacgcct
     1741 tggaaactgc tgaggtcgcc ctggttcatg ccggcgattg ccgccgccag cttcttcttg
     1801 tcccccactt tctggaggtc aaacacccag cgttcggtgc catcccacag cacgacgccc
     1861 atttcatccg tggggccgag cgcagccagc acgccctggg cgcacatgcg ggcgacctcg
     1921 ttgccgttgg cgaactccat gccgtgcatc accagcacca ccgcgccgct gggcaacact
     1981 ttcttgctgt ccaattccat gctgaccggc aaggtcgatt ccagcggggt gccgcgatag
     2041 ccgccggccg cgtaggtctg gtcgccgccc acgcaaacca ggccgacacc gaaatcgcgc
     2101 accacgctct cgagcagcct ctggcgatca agccccagat cgccggcggc aaggttgctg
     2161 atgaagatgg cgtcgtaact ctgcatctcg gccagtgtgc tggggaagtc ctttatgccg
     2221 accagcacca ctccgaggct gggggttttc agggcggccg caagctgctt gtcctgctcc
     2281 ggatcagccg agacaaccag gatgcgcggt tcgccgcgaa cggtggtgaa gctggtggcg
     2341 cggttgttct gggggagcgg gtctccgggc gcatcaacct gcacgtcgta cttgtagaag
     2401 ttgggcttgg acagcgtctg ggggaaggtg aagaggttct tgcccgcgga cagctcgacc
     2461 ttctgctcgc ccaggaattg gtcatcgcgg aagagccgga cggtggcggg ggtggcccgg
     2521 tcagcctgaa cgaagatctt ggcctcgaag acctggcctt ccttgagctt gggcggcaac
     2581 tgcagtttct gcacggatac gtcgttggcg cgcatcacgc ccatgggcac gacgtccacg
     2641 gtcacgccga gaggcttggc ggccaccacg gcgc
//
LOCUS       DATCGS010000575         5990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_643133, whole genome shotgun sequence.
ACCESSION   DATCGS010000575 DATCGS010000000
VERSION     DATCGS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643133"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VIL39_11795"
     CDS             <1..1106
                     /locus_tag="VIL39_11795"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY5482156.1"
                     /translation="PFYGEPPDEHHPWAVHDGNRPPPQEVTPGTFSSETQPGQPPSDA
                     VILFNGTDLSNWESEEKGGPAKWLAKDGVMEVVPKTGQIRTKEQFGDCQLHIEWAEPK
                     DIQGSSQGRGNSGIFLMGICEIQVLDSYHNVTYADGHAASVYGVNPPMANALRPPGEF
                     QVYDIVFRRPVYKEGQLVDPGYVTIFVNGVLAQDHAALEGPTGHMKRSKRTPFPEKGP
                     LALQDHGNPVRFRNIWYRPLPPNAVEGGTDGWLTTEATLAKRKQIAADIRQDAEHLAD
                     PANPVPQLLRLLESLAYDKDDATVQKAEQMASAFVQKVKQLPADQIAAKKDGVKRVAG
                     ALDYLAKNKILPPTFGPKTEADSLIKEQRWDKK"
     gene            1432..2100
                     /locus_tag="VIL39_11800"
     CDS             1432..2100
                     /locus_tag="VIL39_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482157.1"
                     /translation="MSIKLDVPQSGKVGNTVNLRTRFGQIQRQYTIPKDPKSPAQMRI
                     RSNLGHIAPRWRELEQEQRDDWTLAGQDAETRHRLGQSAALKGFNLFLKLNCSRAALG
                     LSQFVVPPPLPQFGANPVGDLSATNDHGVITLKLYVPSAPAQYTVVCGAAPCSAGRSS
                     TQHFNILGFLPAPVEDMSDITDLYVAMYGRIPARSRIFIRTQQHIDGWDDLPKQTTAI
                     VPKG"
     gene            complement(2616..2924)
                     /locus_tag="VIL39_11805"
     CDS             complement(2616..2924)
                     /locus_tag="VIL39_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482158.1"
                     /translation="MEPEEFKADPQQLRAEAEKEPRRRGLEDYAETIQLLKEEKGFSF
                     REIAAWFQQRGVNIDHNAAWRAYSKTTPSDPGGSISEQNERYERKPAREGAMPWLGDS
                     "
     gene            complement(3170..4225)
                     /gene="hypE"
                     /locus_tag="VIL39_11810"
     CDS             complement(3170..4225)
                     /gene="hypE"
                     /locus_tag="VIL39_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEY5482159.1"
                     /translation="MSTNPNFTPSCPFPIQDYPQVLLAHGGGGKLMHQLIGKLFVPAF
                     NNPLLESQHDASVFDIGGQRVAFTTDSYVVKPLFFPGGDLGSLAVHGTVNDLAMAGAR
                     PLYLSAGYIIEEGLPMETLWKIVSSMQQAAQKSGVQIITGDTKVVDKGKGDGLFINTA
                     GIGILEHNQKIAPQSVQAGDAILVSGDVGRHGMAIMAVREGLAFESAIESDSAPVADV
                     VLQLLRAGVEIHCLRDLTRGGLTSVLNEISEAANLAIHVEEKLIPVREDVRAACEVLG
                     LDPFQVACEGRFAAFLPEREAGRALEIMRAHPSAPDACRIGQVSDQRAAKVLIKSTIG
                     PQRILDMSSGEQLPRIC"
     gene            complement(4414..5517)
                     /gene="hypD"
                     /locus_tag="VIL39_11815"
     CDS             complement(4414..5517)
                     /gene="hypD"
                     /locus_tag="VIL39_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEY5482160.1"
                     /translation="MKYLDEYRDADAAHKYARDIARITTRPWSLMEVCGGQTHAIVKF
                     GIDELLPKQITLIHGPGCPVCVTAVELIDKALEIAAWPEVIFCSFGDMLRVPGSKTDL
                     LSVKASGGDVRMIYSPLDAVKLAEQNPSKEIVFFGVGFETTAPTTAMAVYQAAQKGLK
                     NFSLLVSHVLVPPAIEAVLSSPDCRVQAFLAAGHVCAVMGYQEYIPIAAKYHVPIVVT
                     GFEPLDILQGVLMCIRQLESGRALVENQYSRAVRQEGNRPAQQLIREVFQVVPRKWRG
                     IGEIARSGLGLRDAYAAFDAERKFGVAGLCVEESSECLSGLVLQGKIKPHECPAFGTR
                     CTPEHPLGATMVSSEGACAAYYRYRREPTTSAA"
     gene            complement(5637..5858)
                     /locus_tag="VIL39_11820"
                     /pseudo
     CDS             complement(5637..5858)
                     /locus_tag="VIL39_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
BASE COUNT         1173 a         1851 c         1847 g         1119 t
ORIGIN      
        1 cgccgttcta tggcgagccg ccggacgagc accatccctg ggccgtgcac gacgggaacc
       61 gtcccccgcc ccaagaggtc acgccgggca cattcagttc cgagacgcag ccggggcagc
      121 cgccgtctga cgcggtgatt ctcttcaacg gcacggacct ttccaactgg gaatcggagg
      181 agaagggcgg cccggcgaag tggctggcca aagatggcgt catggaagtg gtcccgaaga
      241 ccggacagat tcgcaccaaa gagcaattcg gcgattgcca actgcacatc gaatgggcgg
      301 agccgaagga catccagggc agcagccagg gtcgcggcaa cagcggcatc ttcctcatgg
      361 gcatctgcga aatccaggtg ctggactcct atcacaacgt cacctacgcg gacggccatg
      421 ccgcctcggt ttatggcgtc aacccgccga tggccaacgc cctgcgcccg cctggcgagt
      481 tccaagtcta tgacatcgtc ttccggcgtc cggtttacaa agagggtcag cttgtggacc
      541 cgggctatgt cacgatcttc gtcaatggcg tcctggcgca ggaccacgcg gcgctggaag
      601 gcccaaccgg ccacatgaag cgctcgaagc ggacgccgtt cccggagaaa ggcccgctgg
      661 cgctgcaaga ccacggcaac ccggttcgtt tccgcaatat ctggtatcgc ccgctgccgc
      721 caaacgcagt cgagggcggc acggacggct ggctcaccac cgaggcgacc ctggccaagc
      781 gcaaacagat cgccgccgac attcgccagg atgccgagca cctggctgac ccagccaatc
      841 cggtccccca actgctgcgg ttgctcgaat cgctcgccta cgacaaggat gacgcgaccg
      901 tgcaaaaggc ggaacagatg gccagcgcgt tcgtgcagaa agtgaaacaa ctgcccgccg
      961 accagatcgc ggcgaagaag gacggggtga agcgggtggc cggggctttg gattacctcg
     1021 ccaagaacaa aatcctgccg ccgaccttcg ggccgaaaac cgaggccgac agcctgatta
     1081 aagagcagcg ctgggataag aagtgagttc cccgggcttc gatcgggaaa ttttatggtg
     1141 tgtaactgcc ggtggcagtc gccgctgatt ctcagtctcg aaagatgtcg ccgactgccg
     1201 ggcgtgttgc ccaagcgccc ttttcccgag agtatgttaa cctgcgggtt cgataatgga
     1261 cacagcatcg tgatcctgca ataagaaggt gtggaagtgt cggatcagtg ttgcgcctgc
     1321 aggtcctttc gtgcttaaat aacccatcgg gctggcgggg ttgcgatgag aatctccggc
     1381 gaccggcgcg cggaaggtga acggcgcaac ccgcccagcc tggcaataat tatgagcata
     1441 aaacttgacg ttccacagtc ggggaaggtc ggcaacacgg tcaatctgag gacccgtttc
     1501 ggccagatcc aaaggcaata caccattcca aaggacccca aaagcccggc ccaaatgcgc
     1561 attcgctcca atctcggcca catcgcccct cgctggcgcg aactcgaaca ggagcagcgc
     1621 gacgactgga cgctcgccgg ccaggacgct gaaacccggc atcggctggg tcagtctgct
     1681 gccctcaaag ggttcaacct ctttcttaag ctcaactgct cgcgtgccgc cctcggcttg
     1741 agccagttcg tcgtcccgcc gccgctgccg cagttcggcg ccaacccggt cggcgatctc
     1801 tcggcgacca acgatcacgg cgtgattacc ctcaagcttt acgtgcccag cgcgcccgcc
     1861 cagtacacgg tcgtgtgcgg cgccgccccg tgcagcgcgg gccgatcctc cacccaacat
     1921 ttcaacatcc tcggcttcct gcccgccccg gtcgaagaca tgagcgacat caccgatctc
     1981 tacgttgcga tgtatggccg gatcccggcc cgctcgcgca tcttcatccg cacgcagcag
     2041 catattgacg gttgggacga cctgcccaaa caaaccaccg ccatcgtccc caaagggtag
     2101 gccaggcgtc ccgtctggtc cttgtagctg actcacccgg gcggtgaagg cggcaagctg
     2161 tgtcaatcct tgcgcctttg ccccgcgaag cgtttggagt gcgtccagct tgctggcgct
     2221 atcgtagggc aggggcggtt cgaaagcggg agcaagctcc acgcactcca aacgcttcgc
     2281 gcagttcggt tgcgccagcg gccacgccat ctctttccca cggttgaact ggggtgctta
     2341 agttccaagc tcccacaggc aacccgtctc tgctccgccc atcaacctct acccgcccga
     2401 tccctatgac cgtctaggca catcccctgg agcatcccgc gctatgctat tatggtaata
     2461 tcagggtact atcaaggtac tgttaaggta ctattaaggt actctataca ggctaggcat
     2521 gagagagcgt gttgctccgg tgttgactct gtgtcgctct tggcaagcca cggggatacc
     2581 tcggttaatc cactaaccta ctgtgaatct tgtggttacg aatccccgag ccaaggcata
     2641 gcgccttcac gggctggctt ccgttcgtaa cgttcgtttt gttctgaaat gcttccaccc
     2701 gggtcactcg gggtggtctt tgagtaggcg cgccaggcgg cgttgtgatc aatgttcacg
     2761 cctcgctgct ggaaccaggc ggcaatctct cggaaggaga atcccttctc ttccttcagc
     2821 aactggatcg tttccgcgta gtcttccagg ccgcgacggc gtggttcctt ttcggcctcg
     2881 gcccgcaact gctgcgggtc ggctttgaac tcttccggtt ccatatgttg atatgctagt
     2941 gaatcgtagc gaacagtaaa gaacaaaagt gaacacaacg taatgagtcg gtgaaaacga
     3001 gagaatgcct tggcggagtg cgcccgtcct cgggcgcagc aatcaccgag catggtaatg
     3061 cgtccgatta tattacatct ccatcgcttt ccaaccttgc tgcgcccgag gacgggcgca
     3121 ctccgctaac ttcgtctcgg ccgacactga cccgctacaa taaaatgttt cagcagatac
     3181 gcgggagttg ctcgccgctg gacatgtcga ggatgcgctg ggggccgatg gtgcttttga
     3241 tcagcacctt ggcggcgcgc tggtcggata cctgcccaat cctgcacgcg tcgggggcgc
     3301 tggggtgggc gcgcatgatc tccaaggctc ggccagcctc ccgctcgggc aggaaggcgg
     3361 caaatctgcc ctcgcaggcg acctggaacg gatccaggcc gagcacttcg cacgcggcgc
     3421 ggacgtcctc gcgcaccggg atgagctttt cctcgacgtg gatggcaagg ttggcggctt
     3481 cggagatttc gttgagcacg ctggtcaacc cgccgcgggt gaggtcgcgc aagcagtgga
     3541 tttccacgcc tgcgcggaga agctgcagga ccacgtcggc taccggggcg gaatcgcttt
     3601 cgatggcgct ctcgaaggcc agcccttcgc gaacggccat gatcgccatg ccgtgccggc
     3661 caacatcgcc gctcaccagg atcgcgtcgc ccgcctgcac gctttggggc gcaatcttct
     3721 gattgtgctc aaggatgccg attccggcgg tgttgatgaa gaggccgtcg cctttgccct
     3781 tatccacgac cttggtgtcg ccggtgatga tttgcacgcc cgacttctgg gcggcctgct
     3841 gcatcgagga gacgatcttc cagagggttt ccatgggcag gccttcctca atgatatagc
     3901 cggcactcag gtagagcggg cgtgcgcccg ccatcgccag gtcgttcacc gtgccgtgca
     3961 ccgccaggga cccgaggtcg ccgccgggga agaagagcgg cttcacgacg taggaatcgg
     4021 tggtaaaggc aacgcgctgg ccgccgatgt cgaacacgct ggcatcgtgc tgcgattcaa
     4081 gcagcgggtt gttgaaggcg ggcacaaaca gcttgccgat gagctggtgc atcagcttgc
     4141 cgccgccgcc atgagcgagc agcacctggg ggtagtcctg gatggggaac ggacaggagg
     4201 gggtgaagtt ggggtttgtg gacatgaggg gagttaggaa atccgaaggc cgagggccga
     4261 aatccgaaag aaggccgaaa tccgagaccc gaaatccgaa aggggaggcg tgagatcgac
     4321 gcccgtccca ctgctagtgc taattgggac ctgacgcctg attactgttt tctgcatttt
     4381 cttctggggt tgtggttgtg gttgacggtg tagttacgct gctgaggtag tcggttctcg
     4441 acggtagcgg tagtaggcgg cacaggcgcc ttcgctggaa accatcgttg ctccgagtgg
     4501 atgttcgggg gtgcagcggg tgccgaaggc ggggcattcg tggggcttga tcttgccctg
     4561 caatacgagg ccgctgaggc attcggagga ttcctcgacg cacaggccgg ccacgccaaa
     4621 cttccgctcg gcatcgaagg ccgcgtaggc gtcgcgcaag ccaaggccgc ttcgggcaat
     4681 ctccccgatg ccgcgccact tgcgggggac gacctggaaa acctcgcgta tgagctgctg
     4741 tgccggccga ttgccctcct gccgcaccgc gcggctgtat tggttttcga ccaacgcccg
     4801 cccgctttcc aattgccgga tgcacatcag caccccttgc aggatgtcga ggggctcaaa
     4861 gcccgtcact acgatgggca catgatactt cgcggcaatg gggatgtatt cctggtaacc
     4921 catcaccgcg cagacgtgac cggcggccag gaaagcctgc acgcggcaat caggggaact
     4981 cagcacggcc tcgatggcgg gcggcaccag cacgtgcgag acgagcaggg agaagttctt
     5041 cagacccttt tgggcggctt gatagacggc catcgccgtg gtgggagcgg tagtttcaaa
     5101 gcccacgcca aagaagacga tctccttgga cgggttctgc tcggcgagct taactgcgtc
     5161 cagcggcgaa tagatcatgc gcacatctcc gccgctggcc ttgacggaga gcagatccgt
     5221 cttgctgccg ggcacgcgga gcatgtcgcc gaacgagcag aagatcactt cgggccaggc
     5281 ggcgatctcc agcgctttgt caatcagttc gaccgctgtg acgcagaccg ggcaaccggg
     5341 gccgtggatg agcgtgatct gcttcggcaa cagctcatca atgccgaact tgacgatggc
     5401 atgcgtctgg ccaccgcaga cttccatgag cgaccagggc cgcgtcgtga tgcgggcgat
     5461 gtcccgcgca tatttgtgcg cggcgtcagc atcgcggtat tcgtcgaggt atttcatcgc
     5521 gctggtaggc tggctgccga gggtgagagg gcggggatcg caggcgagac gcctgcgcca
     5581 ctacctgcgc tcgcaggcgg gacgcctgcg ccactatctt gcaggtcaca caactcctcc
     5641 atctcccgca gatactcgaa cacctggttg gcctccgcct catcaacgcg gctgagcgca
     5701 aagccgacgt gcacgatgac gtaatcgccg accttcacct cgggcacgta ggcgaggctg
     5761 acctccctaa ggacgccgcc gaaatcgact ttgccggttc gcatcagcgg gtcgtcgccg
     5821 ctgatgctgg ttacttttcc tgggatagcc aaacacattg cccccaagcc tacactcacg
     5881 gaggcatcgg ggcaatgatg gggcaggggg aaatccgaag gccgaaaccc gagacccgaa
     5941 agaaggccga aggccgaagc ccgaatatgc agccacccca gcagagtcgc
//
LOCUS       DATCGS010000576         4855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_296412, whole genome shotgun sequence.
ACCESSION   DATCGS010000576 DATCGS010000000
VERSION     DATCGS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296412"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..943)
                     /locus_tag="VIL39_11825"
     CDS             complement(158..943)
                     /locus_tag="VIL39_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482161.1"
                     /translation="MLEPELTELEFWLDDVIGGRPLTPTTVDLPTLRGFLEEVEILIK
                     GDVPGASLADSLVRIEPGSLKVVALVAHLLAADVRADLAKLDKTGDLDAIQPKRAQVI
                     ERWQLRARRSPSRVYAVGTGAQKSAIRVTNTSRFQHGGENAWVSVEKHLAGKVVDLGG
                     KQDPNVHLVLADSGESVRVGATEQQLGAEKENQLYKLVNLRVQGEQHLRTKAFRNLRL
                     IQFLPQATEVDEQALASLWEKGRKAWKDVKSDAGWVETLRGNQ"
     gene            complement(1010..1480)
                     /locus_tag="VIL39_11830"
     CDS             complement(1010..1480)
                     /locus_tag="VIL39_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482162.1"
                     /translation="MKLKPSEMEIHDAGHGLKYAVVANIGQEKYTPAENEADLAGKLV
                     AEVADTSADVNVLCRTYYLKREELGRLTGFSLRALAAWAAGELPSQPAKRRLHEVHRL
                     LDALAEIVKREHIPKWLHQRNAVFDDMTPLQVIEFGEIDRLWAMVYDLGSGQPS"
     gene            complement(1706..2308)
                     /locus_tag="VIL39_11835"
     CDS             complement(1706..2308)
                     /locus_tag="VIL39_11835"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY5482163.1"
                     /translation="MPLVTAHKAFPHFFNVLKNNPVLLTPRAEDVFRQGVPRWISRPY
                     RFTGTGSVVSGARFSVKGLMPRLYGSTELATLAAEAYYKSQRAGWTPAQFQPQLTIHM
                     RWELQAVVDLMQAATLNALGVTTNDIVTCDWEKEQTAWLEPVTQAIARAAFENLAEGL
                     VVPSARRTGGLNMVYYPSHRRDGTVIHTLDEAAIPFMHGL"
     gene            complement(2451..2996)
                     /locus_tag="VIL39_11840"
     CDS             complement(2451..2996)
                     /locus_tag="VIL39_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482164.1"
                     /translation="MRTAVPEKIRRIIADINTQGNVSLTRLTVLKKWFEGQGRLPAFG
                     LWIAQQAAGRKGKTKDEAGTLLDEARALLGSAATRESPYQQINRAAAKSLHDRARDLQ
                     NEFQNQQWGPVRIVHCWPLLLVEEGLALHLDRAVTPSDGYRLAADWAQNYDSRYGNGL
                     NGPSRAKLEQLVRFMFTVEAR"
     gene            complement(3004..3528)
                     /locus_tag="VIL39_11845"
     CDS             complement(3004..3528)
                     /locus_tag="VIL39_11845"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEY5482165.1"
                     /translation="MQAGEEFPGGVPYIRTGDIVDGEVVTAGLRHTDPEIAAKFRRSR
                     VEAGDIVMSIRATVGTTALVPAELDGANLTQGTGKISPGDSTERLYLLSFLRASGTQH
                     WISLQIKGATFREITLTRLRELPVAVPPIELQHEFARRVGAVEKLKTAQRASLAELNA
                     LFASLQHRAFRGEL"
     gene            complement(3556..4134)
                     /locus_tag="VIL39_11850"
     CDS             complement(3556..4134)
                     /locus_tag="VIL39_11850"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEY5482166.1"
                     /translation="MSNVVTSWPMVSLFDFCNPKQWPTIPQTAFVEQGYPVYGANGKI
                     GQYTEFNHEKPTVLITCRGATCGTINVCEAKSYVTGNAMALDDLDESRVSAEYLVYAL
                     RNGSLAQAISGTAQPQITRQSLTAISIPLPPLAEQRRIAEVLDRTEALRAKRRAALAQ
                     FNSLAQSLFLDLFGDPATNPKGWPVRQLQEIV"
     gene            complement(4131..4448)
                     /locus_tag="VIL39_11855"
     CDS             complement(4131..4448)
                     /locus_tag="VIL39_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482167.1"
                     /translation="MITITFNMLENANVPPAKAGTPCHLVSFAKLEEEVQQGMRFGVP
                     PSGSQAHANTLTAKTCDALEKANNQPAKAGTPNRLAPYGVPALAGVAEIQQGMKELEG
                     MLK"
     gene            complement(4492..>4855)
                     /locus_tag="VIL39_11860"
     CDS             complement(4492..>4855)
                     /locus_tag="VIL39_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482168.1"
                     /translation="VLPNHCHALVEAPNILKLLHELGRFHGRTSFAWNGEENTRGRQV
                     FHRATERFMRSERHFFATVNYVHNNPVHHGYVRLWTEWRWSSAADYLAQMGRAEAERV
                     WKAYPLRDYGKKWDDAGI"
BASE COUNT          902 a         1528 c         1349 g         1076 t
ORIGIN      
        1 cgaccgggag tgaatccttc ggtcagcagc aatccacatc ggaatttgct gcgccaggca
       61 atgacgaaaa aaatcaaccg ccgccgcatg atttggccgg tcagggtcgg caaacgaaat
      121 caggaagctg gtgtcgagcg agacctgaat catggcgtca ctggtttccc cttaacgttt
      181 ccacccatcc agcatcggac ttcacatcct tccaggcctt ccggcctttt tcccaaagcg
      241 acgccagtgc ttgctcatcc acttcagtgg cctgcggcag aaactgaatc aaccgaagat
      301 tgcggaatgc cttcgtccgc aaatgctgct caccttgcac ccggagattc accagcttgt
      361 aaagctggtt ttccttttcc gctcccaact gttgctcggt tgctcccacc cgcacgctct
      421 cgcccgagtc ggccagcacc agatgcacgt tcgggtcttg cttgcccccg aggtcaacca
      481 ctttcccggc gagatgcttc tcgacactga cccaggcatt ttcaccgccg tgctgaaacc
      541 ggctcgtatt cgtaacccgg atcgcgctct tctgcgctcc cgtgccgact gcatacacgc
      601 ggctcggaga acggcgtgcc cgtaactgcc atcgctcgat tacctgggcg cgcttcggtt
      661 ggatcgcgtc caggtcgccg gtcttgtcca gcttcgccag gtccgcgcgc acgtcggcgg
      721 ccagcaggtg cgccaccagc gcgaccactt tcaacgagcc gggttcgatg cgcacgagtg
      781 aatctgcaag gcttgcgcct ggcacatcgc ccttaatcag aatctccacc tcttcgagaa
      841 agccgcgcaa cgtcggcagg tccaccgtgg tcggggtcag tgggcggcca ccgatgacat
      901 catcgagcca gaactcgagc tctgtcagtt cgggctcaag caaagtatcg tccgtgcctt
      961 cttctgccac aagttgtttg tggcacagtt gttgtggtgc ggtggttctt caacttggct
     1021 ggcccgatcc aaggtcgtaa accatcgccc acagccggtc tatctcgcca aactcaatca
     1081 cctgtaaggg agtcatgtca tcaaataccg cgttgcgttg atgcagccac ttgggaatgt
     1141 gctcacgttt cacgatctcc gccagtgcat ccaacaaccg atgcacctcg tgcaagcgcc
     1201 gcttcgccgg ctggctcggc agttcacccg ccgcccaagc cgccaatgcc cgcagcgaaa
     1261 atccggtcaa gcgccccagc tcttcgcgtt tcagataata ggttctacac aatacgttca
     1321 cgtcggcgct tgtgtcagca acttcggcca ctaatttgcc agccaagtct gcctcgtttt
     1381 ccgccggggt gtatttctcc tgtccgatgt tcgcaaccac tgcgtatttc aacccatgac
     1441 ctgcgtcgtg gatttccatt tccgacggct tcagcttcac gccagccacc agcgcagcct
     1501 ggggctttca tccgggcaac ttcagcccct tgccgtattg atatgtctta ccaccgctca
     1561 gcttcctggt gagcgcgttc ggggtcgcat aggaaggctt tgccttcggt ttagccgctg
     1621 ctttgtggcc cataatcata gcaaacttac gccatcactc aatcgctgtc cagcagatac
     1681 gcatgcagat gtgcatcttt tgacctcaca acccgtgcat aaaagggatg gccgcttcgt
     1741 ccagagtgtg gatcaccgtc ccgtcacgcc gatggctcgg gtaatacacc atattaaggc
     1801 caccggttcg ccgggctgac gggacaacta aaccctcggc cagattctcg aatgccgccc
     1861 gcgcaatcgc ctgcgtcact ggctcaagcc acgcggtttg ctccttttcc cagtcgcagg
     1921 tcacgatgtc gttggttgtc acaccgagtg cattcagggt ggctgcctgc atgaggtcca
     1981 ccaccgcttg cagttcccag cgcatgtgga ttgtcaactg cggttggaat tgtgcgggcg
     2041 tccatccggc acgctggctc ttgtagtaag cctccgccgc cagggtcgcc agctcagtgc
     2101 tcccataaag cctcggcatc aaccctttca cgctaaaacg tgcgccgcta acaaccgaac
     2161 cggtgccggt gaaccgatac gggcgggaga tccagcgcgg cactccttga cgaaacacat
     2221 cttcagctcg cggagtgagc agcaccgggt tgttcttcag cacgttaaag aagtggggaa
     2281 aggctttgtg agcggtaacc aagggcatgg gttgaatggg ttaaacagtt tgctgcttca
     2341 taactccccg cggaaggcgc ggtgctggag ggtggcgaag agcgcgtcca gctccgccag
     2401 cgacgcgcgc tgcgccagct tcactcggag tccttgcctc atcgctcttc ctaccgggct
     2461 tccacggtga acatgaaccg cacgagttgc tcgagtttgg cccggctggg gccgttcaga
     2521 ccattgccat aacgggagtc gtagttctgc gcccagtcgg cggccagtct gtagccgtcg
     2581 ctcggcgtga ctgcccgatc cagatggaga gccaacccct cttccaccag cagcagcggc
     2641 caacaatgga cgatcctcac cggcccccac tgttggttct ggaactcgtt ctgaagatcc
     2701 cgagcccggt cgtgcaggct cttcgctgcc gcccggttga tctgctgata cgggctctcc
     2761 ctcgtggcag ccgaaccgag caaggcacgc gcctcgtcca gcagggtgcc ggcctcgtcc
     2821 ttcgtcttgc ccttgcggcc cgccgcctgc tgcgcaatcc atagaccgaa ggcaggcaac
     2881 cggccttgac cctcgaacca cttcttcaat accgtcaaac gcgtcagcga cacgtttccc
     2941 tgcgtgttga tgtcagcgat gatccttcgg attttctctg gaacggcggt gcgcatggct
     3001 ggctcaaagt tccccgcgga aggcgcggtg ctggagggag gcgaagagcg cgttcagttc
     3061 cgccagcgac gcgcgctgcg ccgtcttcag cttttccacc gctcctactc gacgggcgaa
     3121 ttcgtgctgc aattcgattg gaggaaccgc gacgggcaat tcacgcagtc tggtgagcgt
     3181 aatctcgcgg aatgtcgccc ccttaatttg aaggcttatc cagtgctgcg ttccagaggc
     3241 gcgcagaaag ctcaggagat acaaacgctc cgtgctgtcg cccggagaga tttttcccgt
     3301 tccttgcgtg aggttggcgc catcgagttc cgccggaaca agtgcggtag ttccaactgt
     3361 agcacggatg ctcatcacaa tgtcaccagc ctcgacgcgt gagcggcgaa atttggcggc
     3421 tatctccggg tctgtatgac ggagtccagc ggtaacgact tctccgtcca cgatatcgcc
     3481 cgtgcggatg tatggaactc cgccaggaaa ttcttctccc gcttgcacga tgccgtatgt
     3541 gacgattgtc tcttcctaca cgatttcttg aagctgtcga accggccagc ccttcgggtt
     3601 ggtggcgggg tcgccgaaaa ggtcgaggaa gagggattgg gcgagggagt tgaattgggc
     3661 gagggcggcg cggcgcttgg cccgcaacgc ctccgtccgg tccagcacct ccgcaatccg
     3721 ccgctgctcc gccagcggcg gcagggggat tgaaatggcg gtcaggcttt gccgcgtgat
     3781 ttgtggctgg gctgtccctg agattgcttg agcaagactc ccgttccgca gtgcatagac
     3841 caagtattcc gcactcactc tcgattcatc caagtcatca agggccatcg cgttgccggt
     3901 gacgtatgac ttggcctcac agacgttaat tgttccgcat gtcgcgccgc ggcaggtgat
     3961 caggactgtc ggcttctcat gattgaactc agtgtattgc ccaattttcc cgtttgctcc
     4021 atagactggg tatccttgtt ccacaaaagc ggtttgcgga atcgttggcc actgctttgg
     4081 attgcagaag tcgaacagtg acaccatggg ccatgaggta acgacgttgc tcacttcaac
     4141 atcccctcca gttccttcat gccctgctga atctccgcca caccggctaa agccgggact
     4201 ccatacgggg cgaggcggtt tggagtcccg gctttagccg gttggttgtt cgccttttcg
     4261 agcgcgtcac aagtcttcgc agtcagtgtg tttgcatgcg cctgactgcc tgaaggggga
     4321 actccgaacc tcatgccctg ctgaacctcc tcctccaatt tggcgaagct gacgaggtgg
     4381 catggagtcc cggctttagc cggcgggacg ttcgcgtttt ccagcatgtt gaatgttatc
     4441 gtaatcatcg tctttgcgga cgccccgccg cctgaaggcg ggactccgaa cctatatccc
     4501 ggcgtcatcc cacttcttgc cataatcgcg gaggggatat gccttccaca cgcgctcggc
     4561 ttccgcacga cccatctgtg cgaggtaatc cgccgcactg ctccagcgcc attctgtcca
     4621 cagccgcacg tagccatgat gcaccgggtt gttgtgaacg taattgacgg tggcaaagaa
     4681 gtgccgctcc gagcgcatga accgctcggt ggcccggtga aacacctggc gaccgcgcgt
     4741 gttttcctcg ccgttccagg caaaactggt gcgtccatga aatcgcccca gctcatggag
     4801 cagcttgagg atgttcggcg cttcgaccag cgcgtgacag tgattgggca atacg
//
LOCUS       DATCGS010000577         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_394743, whole genome shotgun sequence.
ACCESSION   DATCGS010000577 DATCGS010000000
VERSION     DATCGS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394743"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1732)
                     /locus_tag="VIL39_11865"
     CDS             complement(359..1732)
                     /locus_tag="VIL39_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5482169.1"
                     /translation="MPPETRKNGSKGPRILIVDDDPGQRSLLDSFLRSQGFDTVPVAS
                     GEAALEVLHDGEISMMISDVRMPGLSGLETLRRARQEHAVLPILLVTAYADIREAVGA
                     MRDGALNYLAKPIDLDELLTTVQQAIGITKLAPLKFNADRQLPGHVIAKSPLMQAVFH
                     EASLIAASESRILITGESGVGKEVLADVIHAWSARAAGPLVKVNCAAIPETLLESELF
                     GHEKGAFTGAVAQRIGRFETAKDGTIFLDEIADMSPPLQAKLLRVTQEGRFHRVGSST
                     ELRTNARILAATNRILEEEVKAGRFREDLFYRLNVVELNIPSLRERPEDILPLASAFI
                     AEFTHGKARFASSVADCLMRYPWPGNVRELRNAMERAALLSLGELILPEHLPAKVRAV
                     AESAPAVEPQDAERLGEIERLAIVQALRKHDFNRTETAKALGISRRALIYKLQRFREA
                     GYTVDPA"
     gene            complement(1931..3487)
                     /locus_tag="VIL39_11870"
     CDS             complement(1931..3487)
                     /locus_tag="VIL39_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5482170.1"
                     /translation="MNVFLVYVGDDDFSRVLPAALNRSKSDNGRVKVMAFPPLGIQTL
                     APVLRQRGHQVRMFDTCHPQMKAEHIARAAAQEHPDVIALSFLSTTTYPAVKRMARQL
                     KAAAPAIPIIVGGAFATFNADRILMDCADIDCVGRGEGEELLPDYLSHLDDLGSVAGL
                     VWRDAGKVIRNPPRPLLQDLDQFPYPDRTSLPIDYIESLPLDVPAVLSLDKFCTVQTS
                     RGCPYSCIYCDIPALGEGRWRSRSPEHVLGELQQLNDEGYRSLYLTDDHFLINRKRIG
                     AICQGIIERKLQFNWGCEGRADAVGIDQLPIMKEARCDFLAFGVEAGTQKILDRLKKR
                     QTLAQVERAVSEAKRQGIARVHGFFVVGSPDETEADIKESFRFAAHLELDTFGFNRLC
                     AYRGTPLWQEYVDRGIIDDARDWHKWFKCTDIDPTTLPSAVVNRLRAKGYGSLFARRI
                     LKRPIRTAKLLRAFGRHMKTTDIIKLLSSPFRRRTLTRKPELPARMLDLGLAEPVRQL
                     VSLAVPQPAG"
     gene            complement(3556..>4158)
                     /locus_tag="VIL39_11875"
     CDS             complement(3556..>4158)
                     /locus_tag="VIL39_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEY5482171.1"
                     /translation="HFPHTAEQPLRSEFARFPWRKNAAWLRAWQRGQTGYPFVDAGMR
                     ELWTTGWMHNRVRMVVASFLAKNLLLPWQEGARWFWETLVDADLASNTLGWQWTAGCG
                     ADAAPFFRIFNPVSQGAKFDPEGSYVRRWVPELARLPAAWIHRPSEAPPAVLAAAGVE
                     LGRTYPHRVVSPLISREVALEAYQRMRRTPERVESPITGR"
BASE COUNT          787 a         1261 c         1324 g          786 t
ORIGIN      
        1 gtcagccaca cacggtctga ctactgcgtt tttgcatcgt caacgcggac aacatagcag
       61 aagcgctatt ctttgcatgt caccagttgt ggggacaagg ccagtcgctg caaaaaggga
      121 agcaaatcgc agttccggcc cgtgcggcgt aacaggtctt accaacttac gagaacaatc
      181 ggtacgttcc cctctcccgg cctgcggcta ccctctccgt gaaccccggg aacatccaac
      241 atccaacatc gaacgcctaa catccattga ttgcccaaag cgcggccctt ggaggttcga
      301 tgttggatgt tggatgttgc ggggtttcga gggaagtgat ttggtttagc ggcgagagct
      361 aggcgggatc aacggtgtat cccgcttccc ggaagcgctg gagcttgtag atcagggcgc
      421 ggcggctgat gccgagggcc ttggccgttt ccgtgcggtt gaagtcgtgt ttgcgcaagg
      481 cctggacgat ggccaggcgt tcgatctccc ccaagcgctc ggcatcctga ggttccaccg
      541 ccggggctga ctcggccacg gcgcggacct tggcgggcag gtgttcgggc aggatgagtt
      601 cgccgaggga gagcagcgcc gcgcgttcca tggcgttgcg cagctcgcgc acgttgccgg
      661 gccagggata gcgcatcagg caatcggcaa ccgacgacgc aaaccgcgcc ttgccgtggg
      721 taaactcggc gatgaaggcg ctggccagcg gcaggatgtc ttcgggccgc tcgcgcaacg
      781 aggggatatt gagttcgacc acgttcagcc ggtagaacag gtcctcgcgg aagcggccgg
      841 ctttgacttc ctcctcgagg atgcggttgg tggcggccag gatgcgcgca ttggtgcgaa
      901 gttcggtgct ggagccgaca cggtggaaac ggccctcttg ggtcacgcgg agcagtttgg
      961 cctgcagcgg cggggacatg tccgcaattt catccaggaa gatggtgccg tccttcgccg
     1021 tctcgaagcg gccaatgcgc tgcgccaccg cgcccgtgaa ggcccctttc tcgtgcccga
     1081 agagttcgct ttcgagcagg gtttccggta tcgcagcgca gttgacctta accagcgggc
     1141 cggccgcgcg ggcgctccag gcgtggatga catcggcgag cacttccttg cccacgccgc
     1201 tttcgcccgt gatcagaatg cggctctccg aggcggcaat cagcgacgcc tcgtggaaga
     1261 cggcctgcat cagcgggctc ttggcgatta cgtgaccggg caactgccga tcggcgttga
     1321 acttcagggg agcgagttta gtgatgccga tggcctgctg gacggttgtc agcagctcat
     1381 ccaggtcaat cggcttggcc aggtaattga gcgcgccgtc gcgcatggcc cccaccgcct
     1441 cacggatgtc ggcgtaggcg gtcaccagca ggatcggcag caccgcatgc tcctggcgcg
     1501 cacgccgcag cgtttccagg cccgacaacc cgggcatgcg cacgtcggag atcatcatgc
     1561 tgatttcccc gtcatggagg acttccaagg ccgcttcacc cgacgcgacc ggcacggtat
     1621 cgaagccctg gctgcgcagg aacgaatcca gcagactgcg ctgacccgga tcgtcatcta
     1681 cgatcaggat gcgcggcccc ttcgaaccgt tcttgcgagt ttcgggtggc atgtgtgcgg
     1741 atctattact gttacacagt tacacagtta cacagttaca tggttacatg gttacatgag
     1801 ttacacgagt tacatgagtg gtccgcgtca ctggcatttg gcaccatgta acctatgtaa
     1861 cctatgtaac ctatgtaacc tatgtaacgc atgtaaccag tttaaccagg taacgcaccg
     1921 ctgttccggt ttagcccgcc ggctgtggaa ctgcaagcga aaccagttgc cggactggct
     1981 ctgcgagacc caaatccagc atccgggccg gcagctcggg tttgcgcgtt aacgtgcgcc
     2041 ggcggaaagg actgctcagc agtttgatga tgtcggttgt cttcatgtgg cggccaaagg
     2101 cacggagcag cttcgctgtt cggattggac gtttcagaat ccggcgggca aagagagagc
     2161 cataaccttt cgcccgcagg cgattaacca ccgcgctggg cagagtggtt gggtcaatgt
     2221 ccgtgcactt gaaccatttg tgccaatccc gcgcgtcgtc aatgattcca cggtccacat
     2281 actcctgcca gagcggggtt ccgcggtagg cgcataagcg attgaacccg aaggtgtcca
     2341 gttcgagatg cgcggcgaag cggaagcttt ccttgatatc cgcttcagtt tcgtcgggcg
     2401 agccgaccac gaagaacccg tgcacccggg cgatgccttg ccgcttggct tcgctgaccg
     2461 cgcgctcaac ctgcgcgagg gtctgccgct tcttcaaccg gtccaggatc ttttgcgtgc
     2521 cggcctcgac gccaaaggcc aggaaatcgc aacgggcctc cttcataatc gggagctgat
     2581 caatgcccac cgcatcggcc cggccttcgc acccccagtt gaactgcagt ttgcgttcaa
     2641 tgatgccctg gcaaatggcg ccgatccgct tgcggttgat gagaaagtga tcgtcggtga
     2701 ggtaaagaga gcgatagccc tcatcgttga gctgctgcag ttcgcccagg acatgctccg
     2761 gcgagcggct ccgccagcgg ccctctccca gcgccgggat atcgcagtaa atgcaggagt
     2821 aggggcagcc ccgggacgtc tgcacggtgc agaacttgtc cagcgaaagg accgcgggca
     2881 catccaacgg cagggattcg atgtagtcaa tcggcaggct ggtgcggtcg ggatacggga
     2941 actggtccag atcctgcagg agcggccgtg gcggattccg aatgactttc cccgcgtcac
     3001 gccagaccag ccccgccact gagccaaggt cgtcgaggtg gctcaaataa tccggcaaaa
     3061 gctcttcgcc ctccccacgg ccaacgcagt caatgtccgc gcagtccatc aggatacggt
     3121 ccgcattaaa agtggcaaag gcgccgccca cgatgatcgg gattgccggc gccgcagcct
     3181 tgagctgccg ggccatgcgc ttgaccgccg ggtaggtagt ggtcgagagg aaagagaggg
     3241 caataacgtc agggtgctcc tgtgcggcag cccgggcgat gtgctccgcc ttcatctgcg
     3301 ggtgacaggt gtcaaacata cgcacctggt gaccccgttg gcggagaacc ggagcgagcg
     3361 tttggattcc caggggaggg aacgccatca ctttaacccg tccgttgtct gacttcgacc
     3421 ggttcagtgc ggcaggcagt actcgggaaa agtcgtcgtc gcccacgtaa acgaggaata
     3481 cattcatttg cacaggactc ttggagaggt tgataccagc aatttaacag cgattcccaa
     3541 ccgctgcaaa tggttttatc tgccggtgat gggcgattcg acgcgctcgg gcgtccgtct
     3601 cattctttga tacgcctcca gtgccacctc gcgggagatg agcgggctga cgacgcgatg
     3661 gggataggtg cggcccagtt cgacccccgc agccgcgagc acggcgggag gcgcttccga
     3721 aggccggtgg atccatgccg cgggcagccg cgccagctcg ggcacccagc ggcggacgta
     3781 gctgccctcc gggtcgaact tcgcgccctg gctgaccggg ttgaagatgc ggaagaacgg
     3841 cgcggcgtcg gccccgcagc cggcagtcca ttgccagcca agagtgttgc tggccaggtc
     3901 ggcatcaacc agcgtctccc agaaccaccg ggcgccctcc tgccagggga gcaagaggtt
     3961 cttcgccagg aacgacgcca ccaccatccg cacacgattg tgcatccagc cggtggtcca
     4021 gagttcgcgc atgccggcgt ccacgaacgg atagccggtc tggccgcgct gccaggcgcg
     4081 gagccacgcg gcgttcttcc gccacgggaa ccgggcgaac tcggaccgca gtggctgctc
     4141 cgccgtgtgt gggaagtg
//
LOCUS       DATCGS010000578         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_395196, whole genome shotgun sequence.
ACCESSION   DATCGS010000578 DATCGS010000000
VERSION     DATCGS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395196"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..305
                     /locus_tag="VIL39_11880"
     CDS             21..305
                     /locus_tag="VIL39_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEY5482172.1"
                     /translation="MRASDYRQPLRAINFICNAPQAKTVSLVGDFNQWNPAAHPMKQM
                     PDRCWLLQVELKHGHHRYAFLVDGVLTLDPRAQGITRNDKGERVSLVPVS"
     gene            450..1757
                     /locus_tag="VIL39_11885"
     CDS             450..1757
                     /locus_tag="VIL39_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5482173.1"
                     /translation="MNTPSNNSLTSRREFLKASALVGSMLAAPAILPGNLFAKENTDT
                     LRVGLIGCGGRGSGAAGQALSADKNVVLTAMGDAFEDQLQNSLHSLQKAHPDKVKVTP
                     EKCFVGLDAYQKVIDSGVDVVLLATPPGFRPVHLKAAVDAGKHIFCEKPVATDAPGIR
                     SVLDSVKAAKEKSLSLVAGFCWRYEVARREFYRRVHDGAIGDIRAVYATYYTGPVKPM
                     PTADSRPASMGDLEWQMRNWYNFVWLSGDGYVEQAIHSVDKVAWAMKDQPPLKAVAVG
                     GRQTPNNQGDIFDHMFVVYEYPDDVRAFVGQRQIAHTYSENSDYLMGSTGFGRIPALY
                     LKGKENWRYKAKDQEGKTDMYQNEHDELFASIRSGKPINDGERMAHSTLLGIMGRMAA
                     YTGQEITWEQAMNSQEKLVPDQLDWKMKLDIAPMAMPGTTKLI"
     gene            1798..2217
                     /locus_tag="VIL39_11890"
     CDS             1798..2217
                     /locus_tag="VIL39_11890"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5482174.1"
                     /translation="MAVEINITYEGGLHCAAIHGPSKQTLTTDAPVDNGGKGAAFSPT
                     DLVATALGACMTTIMGLVAQRNNLNIDGLQVQVLKEMTADPVRRIGSLKTRLVFPRGK
                     LLSVADRAKFEAAAKACPVKQSLHPDLKTPMEFVYPD"
     gene            complement(2221..>2616)
                     /locus_tag="VIL39_11895"
     CDS             complement(2221..>2616)
                     /locus_tag="VIL39_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5482175.1"
                     /translation="GTNELDMQSASFVFEKDGCGFTLKDSQHEYPIACGMGRWQRGET
                     ALPGTPPRLVSGGAPRSGTKSRVATSGAWIDQNTFQMVLRYYETPHHDTVTCRFEEGK
                     VQISFMNSMAQMSPNPNDKRPVLQGRMVG"
BASE COUNT          539 a          789 c          758 g          530 t
ORIGIN      
        1 ataacttgct acgctgagct atgcgtgctt ccgattaccg ccagcccttg cgggctatca
       61 acttcatctg caacgctcca caagccaaga ccgtcagcct cgtcggcgat ttcaaccaat
      121 ggaacccggc cgctcatccg atgaaacaga tgccggaccg ttgctggctg cttcaggttg
      181 aattgaagca tggccaccat cgctatgcgt tcctggtgga cggagtcctg accctcgatc
      241 cccgcgccca gggcatcacc cgcaacgata aaggcgaacg cgtctccctc gtgccggtga
      301 gctgaacatt gcggcgttcg aggaacgcca ggatcagtcg taaagtcaat tggcccatag
      361 ccggtagcgt tgcatggagg cggcgcacgg cgatttaggg gtttacttgg cttgcatttt
      421 cggtctaaat ggccgtcaac atttgaatca tgaatacgcc atccaataat agtctcacct
      481 ctcgccgtga attcctcaaa gcttctgcct tggtgggcag catgctggcc gcacctgcaa
      541 ttttgcccgg gaacctcttc gccaaagaaa acaccgacac cctgcgcgtc ggcttgatcg
      601 gttgcggagg gcgcggctcg ggtgccgccg gccaggctct cagcgccgac aagaacgtcg
      661 ttctcaccgc aatgggcgat gcgttcgagg atcaattgca gaacagtctg cacagcctgc
      721 aaaaggcgca tccggacaag gtcaaagtca ctcccgagaa gtgcttcgtg gggctggacg
      781 cctaccagaa ggtcatcgac agcggcgtgg acgttgtgct gctggccacg ccgccgggtt
      841 ttcgccctgt ccatctcaag gcggcggttg acgcgggcaa acatatcttc tgcgagaagc
      901 cggtggctac cgatgccccg ggcatccgct cggtgctcga ttctgtcaaa gccgcgaagg
      961 agaagagcct gtcgctggtg gccggttttt gctggcgtta cgaggtggcg cggcgcgagt
     1021 tttaccggcg ggttcacgat ggtgcgattg gcgacattcg cgccgtctat gccacttact
     1081 ataccggtcc cgtcaaaccg atgccgaccg ccgactcgcg tcccgccagc atgggggatt
     1141 tggaatggca aatgcgcaac tggtacaatt tcgtctggct cagcggcgat ggttacgtcg
     1201 agcaggccat tcacagcgtg gacaaagtgg cttgggccat gaaggatcag ccgcccctga
     1261 aagccgtggc cgtcggcgga cgtcagacgc ccaacaacca gggcgacatc ttcgaccaca
     1321 tgttcgtcgt ttacgagtat cccgatgacg tgcgcgcctt cgtcgggcag cgccagattg
     1381 cccacacgta ttcggaaaac tcggattatc tgatgggcag cactggtttt ggccggattc
     1441 ccgctcttta cctgaagggc aaagagaatt ggcgatacaa ggccaaggac caggagggga
     1501 agaccgatat gtatcagaac gagcacgacg agctatttgc cagcatccgc agcggcaagc
     1561 cgatcaacga cggcgagcgg atggcgcaca gcactttgct cgggatcatg ggccgcatgg
     1621 ccgcttacac cggccaggaa atcacctggg agcaagccat gaactcgcaa gagaaactcg
     1681 tgcccgacca gctcgactgg aagatgaagc tggacatcgc cccgatggcc atgccgggca
     1741 cgaccaaact catatagagg tttatagtgc gccgcaaatc tgatcgttca tcttgttatg
     1801 gctgtagaaa tcaacatcac ttacgagggt ggacttcact gcgccgcaat ccatggaccg
     1861 tcgaagcaaa ccttaaccac cgacgccccg gtggataatg gcggcaaggg cgcggccttt
     1921 tcgcccacgg acctggtcgc gacggcgttg ggcgcctgca tgacaaccat catgggattg
     1981 gtggcacagc gcaacaacct caacattgat ggactccagg tccaggtgct caaggagatg
     2041 accgccgatc cggtccgccg catcggctca ctgaagacgc ggctggtttt ccctcgcggc
     2101 aaactgttgt ctgtcgccga ccgggccaag ttcgaagccg ccgccaaagc ctgcccggtg
     2161 aaacaaagcc tccatcccga cctcaagacc ccgatggagt ttgtctatcc ggattagccg
     2221 ttatcccacc atccgccctt gcagcacggg gcgcttgtcg ttggggttag gactcatctg
     2281 ggccatgctg ttcataaagc tgatctgcac tttgccctcc tcaaaacggc atgtcacggt
     2341 gtcgtgatgc ggggtctcgt agtagcgcaa aaccatctgg aaggtgttct ggtctatcca
     2401 cgcgccactg gtcgcgaccc ttgatttcgt gccgctcctg ggcgcgccgc ctgaaacgag
     2461 tctcggcgga gtgccgggta gcgcggtctc tccgcgctgc catcgcccca taccgcacgc
     2521 gatgggatat tcatgctggc tgtctttcaa cgtgaagccg cagccgtcct tctcgaacac
     2581 gaatgatgcg ctctgcatgt ccagttcatt ggtgcc
//
LOCUS       DATCGS010000579         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_246736, whole genome shotgun sequence.
ACCESSION   DATCGS010000579 DATCGS010000000
VERSION     DATCGS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246736"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..621
                     /locus_tag="VIL39_11900"
                     /pseudo
     CDS             298..621
                     /locus_tag="VIL39_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(689..2383)
                     /locus_tag="VIL39_11905"
     CDS             complement(689..2383)
                     /locus_tag="VIL39_11905"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEY5482176.1"
                     /translation="MYQADVEYLDRVGPDSFYGQLSQARSRLFRDDDFAALYCPDKGR
                     PAVPPSLLAVALLLQAHDKVSDAEAIARANYDVRWCVALGIEIAIEPFVKSTLQLFRS
                     QLLIHEQAGAIYQASLEEAKRHGFMRGKKRTIALDTTPIFGKGAVKDTYNLLATGLVM
                     LVRERAGAASELPADWAASHEFSLYFGSSIKGEAAINWDQSAERKALLASIVGDAQRL
                     LQLAREALVGLHPGDPAVEAMRKSAALLCQLLVQDIDGLDTEAPALKDGVSKERMPSA
                     TDPEMRHGRKSKRSRFDGHKAALAVDTETGLITEADVIAGNAPDNQNALELVTGTEEN
                     TGCEVDKTLGDGAYGDGATRQEFAEAQRELVARTPGRPEGQLSKDEFDIDLQRDCATC
                     PGGHTMPGSMSRSREGQRWRRLQFPAAVCAACPLRPQCVKGKGGRLVTIHPQEALLQR
                     ARAYEATDEFREDVIRRQVVEHRLARLTQLGIRKSRFFGRQKTKFQLLMASAVANLTL
                     VWGKPAAADESDAEPAPNVLCVARFWRHSALAWAIRRIMSRPAPRAGFANAAHAAA"
BASE COUNT          488 a          982 c          935 g          554 t
ORIGIN      
        1 ccgccgcatc gtcggtttga tcagtcccat gcgctccgcg gttgcccata caaaatgaag
       61 atagatctcg actctggatt gacgcatcat ttcccccgtt tgggccggtg gccgagcgtc
      121 cgttggacgc gaccgtccta cggacgggaa gggtattggt ggcccctgtc ccggacgcta
      181 agagggtgta tgaaaagtcg gttgggcacg gtataatgcc ctggtcaggt cgtcgtagcc
      241 gcgaaggttg ttttgaggtc acagtccaat acacagcaca caccggagcc aggactgatg
      301 ccgacgagcc cattgcctca gggcgccgcg cccagttatc ctagcgacat gaccgaggag
      361 gaatggcggg ttcttcaacc gctgattcca cccgccaagg ccggcggtcg cccccgcggc
      421 gtcgatatgc gcgccgtgat caatggcatc ttctatgtgc tgcgcagcgg tggcgcgtgg
      481 cggatgctgc cccacgactt cccgccctgg ccgacggtgt attactactt ttggcgctgg
      541 cgcggcgacg gcacctggga gcggctccaa gacagcttgc gtactcaggt tcgcgagcgg
      601 gcgggccggg aggccagccc tagaaacctg gccggaaacc cggttttccg gcggatggct
      661 ccgggttggt ggccgtgtga ggtggcggtc aggctgcggc gtgggccgcg ttcgcgaagc
      721 cggccctcgg tgcggggcgg ctcatgatac gcctgatggc ccatgcgagc gcgctgtgcc
      781 gccagaaccg ggcgacacag agcacgttgg gagccggttc ggcgtcgctc tcatctgccg
      841 cagcgggttt gccccacacc agcgtcagat tcgccaccgc cgaggccatc agcagctgga
      901 acttggtctt ctggcggccg aagaagcggc tcttgcggat gcccagttgc gtgagccggg
      961 cgaggcgatg ctccacgact tggcgcctga tgacgtcctc gcggaactcg tcggtggcct
     1021 cgtaggcgcg cgcccgttgc agcagcgctt cttgcgggtg gatcgtgacg agccggccgc
     1081 ccttgccctt gacgcattgc ggtcgcagcg gacacgcggc gcagactgcg gcgggaaatt
     1141 gcaaccggcg ccaccgctgg ccttcgcgcg aacgggacat cgagcccggc atcgtatggc
     1201 cgccggggca ggtggcgcag tcgcgctgca ggtcgatgtc gaactcgtcc tttgacagtt
     1261 ggccttcggg tcggcctggc gtgcgtgcca ccagttcgcg ctgtgcctcc gcgaactcct
     1321 gccgcgttgc gccgtcgccg taggcgccgt cgccgagggt cttgtccacc tcgcagccag
     1381 tgttctcttc ggtgccggtc acgagctcca gagcattctg attgtccggg gcgttgcccg
     1441 cgatcacgtc ggcctcggtg atgaggcccg tctcggtgtc caccgccagc gctgccttgt
     1501 gaccgtcgaa gcgactacgc ttgctcttgc gcccatgccg catctcgggg tcggtcgccg
     1561 acggcatgcg ctccttgctc acgccgtcct tcaacgccgg cgcctcagtg tcgaggccgt
     1621 caatgtcctg caccaagagc tggcacagga gcgcggcgga cttgcgcatt gcctcgactg
     1681 ccggatcacc cgggtggagg ccgaccagcg cctcgcgcgc cagttgcagc agtcgctggg
     1741 cgtcgccgac gatgcttgcc aggagcgcct tgcgctcggc tgactggtcc cagttgatcg
     1801 ccgcttcgcc cttgatgctg ctgccgaaat agagcgagaa ctcatgactg gcggcccagt
     1861 ctgcgggcaa ctcactggcc gcccccgccc gctcgcgcac cagcatcacg aggcccgtcg
     1921 ccagcagatt gtaggtatct ttcaccgcgc ccttgccgaa gatcggcgtc gtgtccaagg
     1981 cgatcgtgcg cttcttgccg cgcatgaaac cgtggcgctt ggcttcttca aggctcgcct
     2041 gatagatcgc accggcctgc tcatggatca gaagctgaga ccgaaacaac tgcagcgtgc
     2101 tcttgacgaa cggctcgatc gcaatctcaa tcccgagcgc cacacaccaa cgcacatcgt
     2161 agttcgcgcg cgcaatcgct tccgcatccg acaccttgtc atgggcctga agcagcaagg
     2221 ccaccgccaa caggctcggc ggcacagcag gccgaccctt gtccgggcag tacagcgcgg
     2281 caaaatcgtc atcccgaaac agccgcgacc gcgcctgcga caactggccg tagaagctgt
     2341 cgggacccac ccggtccagg tactccacat ccgcctggta catactcagc tgcgatgagc
     2401 gcggcgatag catcgcaaga accccttcat ctgcggatga agggaagttc tacagcccca
     2461 agatcatgcc tgcacagacc gggcttttcg gccaggtttc tagtgtcttg tccgttaaat
     2521 atataaacag ttaggaaagg atttgggctg gcttgtggaa taggcagatg atctcactaa
     2581 ggagtgactt atgtctggtc cacgagccgt accgatcctc ctctccgacg ccgaccgtca
     2641 gcaccttgag aagacaaccc gtcagttgac tgcgccccag cggatggttc tacgcagccg
     2701 catcgtgttg ctcgccgccg ggggccttgg caactcggcc atcgggcgac aacttgcctg
     2761 caatcgcgcc accgcaattc tgtggcggag acggttcgcc gagcacggct tagccgggct
     2821 gttggacgag ccgcgaccgg aacgcccccg gtctttttcc cccacagcag cgccatgagg
     2881 tcatcgctct ggcctgcagc tatccgccgg atcatggctt tgccatcacc cactggagcc
     2941 tgcgcacgct ggcccgcgc
//
LOCUS       DATCGS010000580         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_246795, whole genome shotgun sequence.
ACCESSION   DATCGS010000580 DATCGS010000000
VERSION     DATCGS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246795"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1571
                     /gene="gatA"
                     /locus_tag="VIL39_11910"
     CDS             99..1571
                     /gene="gatA"
                     /locus_tag="VIL39_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY5482177.1"
                     /translation="MLNQLTISALVAKLARREVSARAVTQACLDRIERVDPQIRAFIS
                     YDAADALAQADAADQALAQGQTHTQKPLLGVPVGIKDVIAVKGQPLNCGSKILGEFRS
                     PYDATVIEKLKTAGAVVFGRLNMDEFAMGSSTENSAFQITRNPWDTSRIPGGSSGGSA
                     AAVVADECVAALGSDTGGSIRQPAALCGCVGLKPSYGRVSRYGLVAYASSLDQIGCFA
                     KDVRDAATMMGVIGGHDPRDSSSVPQPVPSYPAALTGDIKGLKLGLPKEYMIGGLDPE
                     VKQAVEAAVQQLAKLGAEVVEVSLPHTDYAIATYYIIATAEASANLARFDGIRYGKRV
                     EGADPIALYGKTRGAGFGPEVKRRIILGTYVLSSGYYDAYYLRAQKVRTLIRNDFLKA
                     FEKVDAIVTPTSPTAAFKIGEKAEDPLQMYLMDIFTISCNLAGICGLAVPCGFTGPQP
                     RLPIGLQLLGKPFGEETILRIAHAYEQSTSWHKEKPPIKG"
     gene            complement(1641..>2072)
                     /locus_tag="VIL39_11915"
     CDS             complement(1641..>2072)
                     /locus_tag="VIL39_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5482178.1"
                     /translation="NVFACPNAPKWPAPAPGAWYNTDYGNNHNEANLPGASQQAWYQA
                     HPDFGFNESITMSSLRRPSDFIMLGDAGRSDGTASRGGMYPQPWAFDNSTQARMLGRH
                     HKGIANLTYSDGHAFGSITNKTWRTEQDNNWRRYQITTDIK"
BASE COUNT          376 a          661 c          638 g          397 t
ORIGIN      
        1 ctccatttcc tggcgcccca tttcccgctc gcttcttgcc gccaacttgg ccactttgca
       61 cgcagtgctg cggccggatt gtgggctgcg gctatgtcat gctgaaccaa cttacgattt
      121 ccgcgctcgt ggcgaagctg gccaggcgcg aggtgtccgc gcgggccgtc acgcaagctt
      181 gcctggaccg gattgagcgg gttgatcccc agatccgcgc ctttatcagt tacgacgccg
      241 cagacgcgct ggcccaggcc gacgccgccg accaggcgct cgcgcaaggc cagacccata
      301 cccaaaaacc gctgcttggc gtgcccgtag gcataaaaga tgtcatcgcc gtaaagggac
      361 aaccgcttaa ctgtggatct aagatcctcg gcgagtttcg ctcgccctac gacgcgaccg
      421 tgatcgagaa gctgaagacc gctggggcgg tggtgttcgg ccggctgaac atggacgagt
      481 tcgccatggg cagttcaacg gagaattcgg cgttccaaat cacccgcaat ccctgggaca
      541 catcccgcat tcccggcggg tcctcgggcg gttccgcggc ggcggtcgtg gccgatgaat
      601 gtgtcgccgc gctggggtcg gacacgggcg gctcgattcg ccagccggcc gctttgtgcg
      661 gctgcgtcgg cctcaaaccc tcctatggcc gcgtctcacg ttatgggctg gtggcctacg
      721 cgtcatccct cgaccagatc ggctgtttcg ccaaagacgt gcgcgacgcg gcgacgatga
      781 tgggcgtcat cggcggccac gatccccgtg actcctcgag cgtgccgcag cccgtcccga
      841 gctaccccgc cgccctgacg ggtgacatca aggggctgaa gctgggcctg ccgaaggagt
      901 atatgattgg cggcctggac cccgaggtga agcaggccgt cgaggccgcg gtgcaacagc
      961 tcgccaaact gggcgcggag gtggtcgaag tctccttgcc ccacaccgac tacgccatcg
     1021 cgacctacta catcatcgcc accgccgagg ccagcgccaa tctggcgcgc tttgatggca
     1081 tccgttacgg caaacgcgtc gagggagcgg acccaatcgc gctctatggc aagacccggg
     1141 gcgccgggtt cggccccgaa gtgaagcggc gcatcatcct gggcacctac gtcctgagca
     1201 gcggctatta cgacgcctat tacctccgcg cgcagaaggt gcggacattg atccgcaacg
     1261 acttcctgaa agcattcgag aaggtggatg ccatcgttac gccgacctcg cccaccgccg
     1321 ccttcaagat cggcgagaag gccgaggacc cgttgcagat gtacctgatg gatatcttca
     1381 ccatctcctg caacctggcg ggcatctgcg gcctggcggt gccctgcggc tttaccggcc
     1441 ctcaaccgcg cctccccatt gggctccagc ttttgggtaa accgttcggt gaggaaacga
     1501 ttttgcgcat tgctcacgcc tacgagcaga gcacgagctg gcacaaagaa aagccgccga
     1561 tcaaaggtta gctcgtgacg tgaagcgtct cgatcccaaa tcgcctgccg cctgccacct
     1621 cgccgcaggt cgctatatag tcacttaatg tcggtggtga tctggtaacg gcgccagttg
     1681 ttgtcttgct ccgtccgcca ggtcttgttc gtgatggaac cgaaggcatg gccatcggaa
     1741 taggtgaggt tggcgatccc cttgtgatga cgtcccagca ttctggcctg agtactgttg
     1801 tcaaaggccc acggctgcgg atacatcccg ccacgggagg cagtgccgtc ggagcgtccc
     1861 gcgtcgccca gcatgatgaa gtcagaggga cgcctcagag aactcatagt tatgctttca
     1921 ttgaagccaa aatcagggtg ggcttgatac caggcctgct gtgacgcccc gggcagattc
     1981 gcttcgttat ggttgttgcc gtaatccgtg ttgtaccacg cgccgggcgc cggagcgggc
     2041 catttgggcg cattcgggca ggcgaaaaca tt
//
LOCUS       DATCGS010000581         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U7813
            k141_246813, whole genome shotgun sequence.
ACCESSION   DATCGS010000581 DATCGS010000000
VERSION     DATCGS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962184
            Sequence Read Archive: SRR3989121
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 15:42:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,387
            CDSs (total)                      :: 2,372
            Genes (coding)                    :: 2,346
            CDSs (with protein)               :: 2,346
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 21 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            Pseudo Genes (multiple problems)  :: 3 of 26
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246813"
                     /isolate="SMAG_U7813"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.29055 N 156.788643 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VIL39_11920"
     CDS             <1..236
                     /locus_tag="VIL39_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5482179.1"
                     /translation="SRLWTVQTPQTFRVDIIRRALSAVREKGLLVTDDTAACELIGQP
                     VQLVLSTEPNPKVTRPEDLTVVEALLRSMAPRA"
     gene            534..1865
                     /locus_tag="VIL39_11925"
     CDS             534..1865
                     /locus_tag="VIL39_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5482180.1"
                     /translation="MKRRLLYGVVITVLSFNLLIGARVYLSSAQAAEKDSAYPSLELF
                     STVMEKVRKDYVEGQNLKYQDLVYAALKGMLNTLDPHSEFMEPEKYKELQSDTQGAFG
                     GLGIVISMKDNFITVISPMEDSPGFKAGITSGDRIIKIDGKSTEKMSTQDAVKILRGE
                     PGTDVKISILRPSSGQVKDYKLTRAVITVDMVKDINGKKEFPISDNKIGYIRLVQFGE
                     KTSADLETALKKLKDEGMQGLVLDLRGNPGGLLDQAVDVCEKFLPRGDLVVTTEGRNP
                     SQNSVRKARGRGDELHNMPMVVLVNLGSASASEIVAGCLQDTKRAIILGEKTFGKGSV
                     QSILPLGDGSALRLTTAKYYTPSHKVIHEEGITPDIVVPVTDEDERDILLRHTPGGLQ
                     SLDDKDRERVLKTRDPQLDRAMDLLKGITLFTQRAPSGDKILAKSGKMAAN"
     gene            1909..2934
                     /gene="tsaD"
                     /locus_tag="VIL39_11930"
     CDS             1909..2934
                     /gene="tsaD"
                     /locus_tag="VIL39_11930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5482181.1"
                     /translation="MPLLAIETSCDETSAAVVHQGRVLSNVVSSQITLHAEYGGVVPE
                     LAAREHLRNLIPVARAALREASVEAGQLDAVAATRGPGLPNALLVGFKAAQAAAFVLG
                     RPFLGIHHHQAHLYSAWLRGQPPVADFSKFQPNLSLIVSGGHTLLVHVRAELDHHVLG
                     STVDDAAGECFDKTGKMIGLPYPAGPEIDRLAETGNPKAYDFPRPMIDDPNNDFSFSG
                     LKTSVRYFLRDHPGLLDDAQRVRDLCASVQAAIVDVLVTKTIRAAKRLRVGCVTASGG
                     VTCNRALRQRLTAACERERLTLLLAERNFCTDNAAMIGILAERKLMLRSEPTTLDADI
                     LPNWALE"
     gene            complement(2959..>3670)
                     /locus_tag="VIL39_11935"
     CDS             complement(2959..>3670)
                     /locus_tag="VIL39_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5482182.1"
                     /translation="AVGVTELPVYADEHLRDAADMTTGANADGFHLRNVALGRDIQVA
                     HWADLRLVQAGEACHKCGKPLKVQRAIEVGHVFKLGTKYSEALKALFLDESGKQQPCI
                     MGCYGIGVTRTMQAVIEQSNDANGIIWPISVAPYTVCITPLGIAPESAVMQQAEKLYA
                     ELTAKGVDVILDDRNERPGVKFKDSELVGFPIRIGIGERSLAKGEMEVKPRAGALIPV
                     KAEEAVEKVLALIAAAAL"
BASE COUNT          722 a         1184 c         1104 g          660 t
ORIGIN      
        1 gttcccggct ctggacagtg caaacccctc agactttccg ggtggacatc atccggcggg
       61 cattatcggc cgtgagggaa aagggcctgc tcgttacgga cgacacggcg gcgtgcgaac
      121 tcatcggcca gccggtccaa ctggtgctca gcaccgaacc gaaccccaag gtcacccgcc
      181 cggaggatct aaccgtcgtg gaggcgctgc tccgaagcat ggcacctcgg gcttgacgca
      241 aaggccggcg gactcaagat ccgaaacccg aagaccgaag gtcgaaggcc gaaagaattc
      301 cgaaggccga aatccgagga tgcctgtccc cgatagactg ccactgtgct gggctggctg
      361 tggactcggg gttcgggttt cggaattcgg gtttccttcg gaattcgggt ttcgagcttc
      421 ggatttcggc gtcggatcga cttctagccc tgaggccggc cgcgcccgcg ggcacggcct
      481 tgcaaactca gccgctaacc actatggtct ggcagattga tttttagagc attatgaaac
      541 gacgtctgct ttacggggtg gtaatcaccg tactcagctt taacctgctg attggcgccc
      601 gcgtttacct gagttccgct caggcggcgg agaaggattc ggcctatccc agcctcgaac
      661 tcttttcgac cgtgatggag aaggtgcgca aggattacgt ggaaggccag aacctcaaat
      721 accaggacct ggtctatgcc gccctcaaag gcatgctcaa caccctggac ccgcacagcg
      781 agttcatgga gcctgagaag tacaaagagc tgcagagcga cacccagggc gcctttggcg
      841 gcctcggcat cgtgatctcc atgaaggaca acttcattac ggtcatctcc cccatggaag
      901 acagtcccgg cttcaaagcg ggcatcacgt ccggagaccg catcattaag attgacggca
      961 agagcaccga gaagatgagc acgcaggacg cggtcaagat cctgcgcggc gagccgggca
     1021 ccgacgtgaa gatcagcatc ctccgcccct cctcgggcca ggtcaaagat tacaagctca
     1081 cccgcgccgt catcaccgtt gacatggtga aagatatcaa cggcaagaag gaattcccca
     1141 tcagcgacaa taagatcggc tacatccggc tggtccagtt tggcgagaag accagcgccg
     1201 acctggagac cgcgctcaag aaattgaaag acgaaggcat gcaggggctg gtgctggacc
     1261 tgcgcgggaa tccgggtggc ctgctcgatc aggccgtgga tgtctgcgag aaattcctgc
     1321 cccgcggcga tttggtggtg acgaccgagg gccgcaatcc gagccagaac tccgtgcgca
     1381 aggcccgggg acgcggcgac gagctgcaca acatgccgat ggtcgtgctg gtcaacctgg
     1441 gcagcgccag cgcctcggag atcgtcgcgg gctgcttgca ggataccaag cgcgccatca
     1501 ttctcggcga aaagaccttt ggcaaaggct ccgtgcagag catcctgccg cttggtgacg
     1561 gctcggcctt gcgcctcacc acggccaagt actacacccc gagccataaa gtcattcacg
     1621 aggaaggcat caccccggac atcgtggtcc cggtgaccga cgaggatgag cgcgatattc
     1681 tgctcaggca caccccgggg ggccttcaaa gcctcgacga caaggaccgc gagcgagtgc
     1741 tgaagacccg cgacccgcaa ctcgatcgcg ccatggacct cctcaagggc atcacactct
     1801 tcacccagcg cgccccgtca ggagacaaga tactggccaa gagcggaaag atggccgcca
     1861 actagtccgc cccacgcatc acgcatcacg catcccgcat cacgctgaat gcctctgctt
     1921 gccatcgaaa cctcgtgtga cgagaccagt gcggcggtcg ttcaccaggg ccgcgtgctg
     1981 tcgaacgtcg tctcgtccca gatcaccctg catgcggaat acgggggcgt cgtgccggag
     2041 ctggcggcac gcgagcacct gcggaacctg ataccggttg cccgggcggc cttgcgcgaa
     2101 gcgtcagtcg aagccggcca actggacgcc gtggccgcca cccgcggtcc cggcctgccc
     2161 aacgcgctct tggtcggctt caaagcggcg caggcggcgg cttttgtctt ggggcgcccg
     2221 tttctaggca tccatcacca ccaggctcac ctttattcgg cctggctccg aggccagccg
     2281 ccggtcgccg atttctcaaa gttccagccg aacctttccc tcattgtcag cggcggccac
     2341 accctgctcg tccatgtccg agccgaactg gaccaccacg tgctcggctc gaccgttgat
     2401 gacgccgccg gggagtgctt tgacaagacc ggcaagatga tcggtttgcc ctatcccgca
     2461 gggccggaaa ttgaccggct ggcggagacc gggaacccca aggcgtatga tttcccccgg
     2521 cccatgattg atgaccctaa caacgacttc agcttcagcg ggctcaagac ctcagtgcgc
     2581 tatttcctgc gcgaccatcc cggcctgctg gatgacgcgc aacgggtgcg cgatttgtgc
     2641 gccagcgtgc aagcggccat tgtggacgtg ctcgtcacca agacgatccg cgcggccaag
     2701 cgtctccgtg tgggctgcgt cacagcctcc ggcggcgtca cctgcaatcg cgccctccgc
     2761 cagagactca ccgccgcctg cgaacgcgag cggctcaccc tgttgctggc cgagaggaac
     2821 ttctgcaccg acaacgcggc gatgattggc atcctggctg agcgcaagct tatgctccga
     2881 tcggagccaa ctactctcga cgcggatatc cttcccaact gggcattgga ataattggga
     2941 atagtcggca ggccccgctt atagtgcggc tgccgcgatc aacgccagaa ccttctccac
     3001 cgcctcttct gccttgaccg ggatgagcgc gccggcgcga ggtttgactt ccatttcacc
     3061 cttggccagc gaccgctcgc caatgccaat ccggatcggg aagccgacca actcggaatc
     3121 cttgaacttc acgccgggcc gctcgttgcg gtcgtcgaga atgacatcga cacccttcgc
     3181 ggtgagctcg gcgtatagct tctcggcctg ctgcataacc gcgctctcgg gcgcgatgcc
     3241 caggggcgta atacagactg tgtaaggcgc gacgctgatc ggccagatga tgccattggc
     3301 gtcgttgctc tgctcgatga ccgcctgcat ggtgcgggtg accccgatgc cgtaacagcc
     3361 catgatgcac ggctgctgct tgccggattc gtcgaggaaa agcgccttga gggcctcgct
     3421 gtacttggtg cccagcttga acacgtgccc gacctcgatg gcgcgttgga ccttgagcgg
     3481 tttgccgcac ttgtggcagg cctcgcctgc ttgcaccagg cgcaggtctg cccagtgcgc
     3541 gacctgaata tcgcggccaa gggcaacatt gcgcaagtga aagccatccg cgttggcgcc
     3601 ggtggtcatg tctgcggcgt cgcgcaggtg ttcgtcagca tagactggca gctccgtgac
     3661 gcccaccgcg
//
